LOCUS       DAZABD010000001        93313 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000001 DAZABD010000000
VERSION     DAZABD010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 93313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 93313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..93313
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.001"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(57..1508)
                     /gene="cls"
                     /locus_tag="ACHALG_000001"
     CDS             complement(57..1508)
                     /gene="cls"
                     /locus_tag="ACHALG_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFN2585450.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQKALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            1850..4516
                     /locus_tag="ACHALG_000002"
     CDS             1850..4516
                     /locus_tag="ACHALG_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFN2585451.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNE
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            4880..5632
                     /locus_tag="ACHALG_000003"
     CDS             4880..5632
                     /locus_tag="ACHALG_000003"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFN2585452.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(5654..6808)
                     /locus_tag="ACHALG_000004"
     CDS             complement(5654..6808)
                     /locus_tag="ACHALG_000004"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HFN2585453.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            6993..9434
                     /locus_tag="ACHALG_000005"
     CDS             6993..9434
                     /locus_tag="ACHALG_000005"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HFN2585454.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKPYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     KVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(9764..10468)
                     /locus_tag="ACHALG_000006"
     CDS             complement(9764..10468)
                     /locus_tag="ACHALG_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2585455.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(10655..10900)
                     /locus_tag="ACHALG_000007"
     CDS             complement(10655..10900)
                     /locus_tag="ACHALG_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2585456.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(10917..11768)
                     /locus_tag="ACHALG_000008"
     CDS             complement(10917..11768)
                     /locus_tag="ACHALG_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFN2585457.1"
                     /translation="MNSLTSTYRLLNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQPTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            11934..12938
                     /locus_tag="ACHALG_000009"
     CDS             11934..12938
                     /locus_tag="ACHALG_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFN2585458.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            complement(13179..13535)
                     /locus_tag="ACHALG_000010"
     CDS             complement(13179..13535)
                     /locus_tag="ACHALG_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFN2585459.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            13903..15090
                     /gene="tuf"
                     /locus_tag="ACHALG_000011"
     CDS             13903..15090
                     /gene="tuf"
                     /locus_tag="ACHALG_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFN2585460.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            complement(15278..17869)
                     /gene="mprF"
                     /locus_tag="ACHALG_000012"
     CDS             complement(15278..17869)
                     /gene="mprF"
                     /locus_tag="ACHALG_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFN2585461.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLVSVLPMIGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDP
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(17895..18656)
                     /locus_tag="ACHALG_000013"
     CDS             complement(17895..18656)
                     /locus_tag="ACHALG_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFN2585462.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            18823..19455
                     /locus_tag="ACHALG_000014"
     CDS             18823..19455
                     /locus_tag="ACHALG_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HFN2585463.1"
                     /translation="MKRKRLSIIRILFYMIILTVIGAVLFLGGRTYRSVKRVESYQIE
                     ITTAAKNYHMENYENLIKAIILTESKGKGTDLMRSSESLHGEAEIIDNPADSIDQGVK
                     YLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHGGENTVELAEAYSKDVLSPLLG
                     NEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKMNEKIIETYEKVFK"
     gene            19514..20374
                     /locus_tag="ACHALG_000015"
     CDS             19514..20374
                     /locus_tag="ACHALG_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HFN2585464.1"
                     /translation="MEKRQKMENQKVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            20559..22874
                     /locus_tag="ACHALG_000016"
     CDS             20559..22874
                     /locus_tag="ACHALG_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFN2585465.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            23056..24897
                     /gene="dnaG"
                     /locus_tag="ACHALG_000017"
     CDS             23056..24897
                     /gene="dnaG"
                     /locus_tag="ACHALG_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFN2585466.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            24921..26030
                     /gene="rpoD"
                     /locus_tag="ACHALG_000018"
     CDS             24921..26030
                     /gene="rpoD"
                     /locus_tag="ACHALG_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFN2585467.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            26247..28373
                     /locus_tag="ACHALG_000019"
     CDS             26247..28373
                     /locus_tag="ACHALG_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division site-positioning protein MapZ
                     family protein"
                     /protein_id="HFN2585468.1"
                     /translation="MMKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDA
                     AMQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIE
                     KNEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHRED
                     TKEEHAKELLKMISEKIAQENKVEPEHEAEKDTEKDISGVNAAGKESLKTNDLKTDIP
                     EKTETLSEKQSEDEKSNGLPEADKKEMLEKADKKATESDGINNEADAETQSASEDSTA
                     LEAKIEKEENQPELIEAETEPITIVPNTEKTPQESNPEKKDLDKTQKKKSKKVPYLAL
                     AAVLLLGGGGWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMI
                     HQDLSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDI
                     KLKADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATR
                     DQYKTAKEAVEKVKNTDLSAQLNEQLQQVDKMLTDKEKKEEEEKKKAEEAKKAEKAKK
                     AAEEQAKQAAQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADA
                     SNPAWTWAPGVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKG
                     IGESALPFYIVTINCKTGWFGGNGSN"
     gene            28569..29432
                     /locus_tag="ACHALG_000020"
     CDS             28569..29432
                     /locus_tag="ACHALG_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor B family protein"
                     /protein_id="HFN2585469.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            29537..30010
                     /locus_tag="ACHALG_000021"
     CDS             29537..30010
                     /locus_tag="ACHALG_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFN2585470.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            30106..30993
                     /gene="xerD"
                     /locus_tag="ACHALG_000022"
     CDS             30106..30993
                     /gene="xerD"
                     /locus_tag="ACHALG_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFN2585471.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            31012..31383
                     /locus_tag="ACHALG_000023"
     CDS             31012..31383
                     /locus_tag="ACHALG_000023"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN2585472.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGVYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            31380..32150
                     /locus_tag="ACHALG_000024"
     CDS             31380..32150
                     /locus_tag="ACHALG_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HFN2585473.1"
                     /translation="MNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDIPIATITEQY
                     MNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEEDPREELVTQ
                     LLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKKIDLIRAMQK
                     ILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESPSKSEVVTTF
                     MALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            32134..32703
                     /gene="scpB"
                     /locus_tag="ACHALG_000025"
     CDS             32134..32703
                     /gene="scpB"
                     /locus_tag="ACHALG_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFN2585474.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISFEEDVSPDLFFDGMNKGE"
     gene            32709..33425
                     /locus_tag="ACHALG_000026"
     CDS             32709..33425
                     /locus_tag="ACHALG_000026"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFN2585475.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     regulatory      33507..33631
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            33754..34365
                     /locus_tag="ACHALG_000027"
     CDS             33754..34365
                     /locus_tag="ACHALG_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFN2585476.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIP
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     gene            34743..36266
                     /locus_tag="ACHALG_000028"
     CDS             34743..36266
                     /locus_tag="ACHALG_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFN2585477.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            36503..37843
                     /locus_tag="ACHALG_000029"
     CDS             36503..37843
                     /locus_tag="ACHALG_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFN2585478.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            37997..38734
                     /locus_tag="ACHALG_000030"
     CDS             37997..38734
                     /locus_tag="ACHALG_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFN2585479.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGNSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(38899..39120)
                     /locus_tag="ACHALG_000031"
     CDS             complement(38899..39120)
                     /locus_tag="ACHALG_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HFN2585480.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            39151..40182
                     /locus_tag="ACHALG_000032"
     CDS             39151..40182
                     /locus_tag="ACHALG_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN2585481.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYERILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            40169..41572
                     /locus_tag="ACHALG_000033"
     CDS             40169..41572
                     /locus_tag="ACHALG_000033"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFN2585482.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGFLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVIHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            41638..42273
                     /locus_tag="ACHALG_000034"
     CDS             41638..42273
                     /locus_tag="ACHALG_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1386/EF1546 family surface-associated
                     protein"
                     /protein_id="HFN2585483.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            42322..43002
                     /gene="cmk"
                     /locus_tag="ACHALG_000035"
     CDS             42322..43002
                     /gene="cmk"
                     /locus_tag="ACHALG_000035"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFN2585484.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            43137..44366
                     /gene="rpsA"
                     /locus_tag="ACHALG_000036"
     CDS             43137..44366
                     /gene="rpsA"
                     /locus_tag="ACHALG_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFN2585485.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            44548..45858
                     /gene="der"
                     /locus_tag="ACHALG_000037"
     CDS             44548..45858
                     /gene="der"
                     /locus_tag="ACHALG_000037"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFN2585486.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            46101..46376
                     /locus_tag="ACHALG_000038"
     CDS             46101..46376
                     /locus_tag="ACHALG_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFN2585487.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            46611..47657
                     /locus_tag="ACHALG_000039"
     CDS             46611..47657
                     /locus_tag="ACHALG_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2585488.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            47659..48918
                     /locus_tag="ACHALG_000040"
     CDS             47659..48918
                     /locus_tag="ACHALG_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFN2585489.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            48924..49505
                     /locus_tag="ACHALG_000041"
     CDS             48924..49505
                     /locus_tag="ACHALG_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ReoY family proteolytic degradation factor"
                     /protein_id="HFN2585490.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(49587..50462)
                     /locus_tag="ACHALG_000042"
     CDS             complement(49587..50462)
                     /locus_tag="ACHALG_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFN2585491.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            50610..50942
                     /locus_tag="ACHALG_000043"
     CDS             50610..50942
                     /locus_tag="ACHALG_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFN2585492.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            51054..52262
                     /locus_tag="ACHALG_000044"
     CDS             51054..52262
                     /locus_tag="ACHALG_000044"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HFN2585493.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            52416..52871
                     /locus_tag="ACHALG_000045"
     CDS             52416..52871
                     /locus_tag="ACHALG_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2585494.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            53233..53508
                     /locus_tag="ACHALG_000046"
     CDS             53233..53508
                     /locus_tag="ACHALG_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2585495.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            53521..55599
                     /locus_tag="ACHALG_000047"
     CDS             53521..55599
                     /locus_tag="ACHALG_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFN2585496.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            55762..57645
                     /locus_tag="ACHALG_000048"
     CDS             55762..57645
                     /locus_tag="ACHALG_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFN2585497.1"
                     /translation="MKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKTS
                     LLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVRA
                     YEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIST
                     LSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDRY
                     FLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMRA
                     GVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRINDQ
                     TLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQE
                     NEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEKR
                     RLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVAD
                     KLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKEW
                     ETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSEW
                     VED"
     gene            57657..58604
                     /locus_tag="ACHALG_000049"
     CDS             57657..58604
                     /locus_tag="ACHALG_000049"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFN2585498.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            58624..59151
                     /locus_tag="ACHALG_000050"
     CDS             58624..59151
                     /locus_tag="ACHALG_000050"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFN2585499.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(59214..59867)
                     /locus_tag="ACHALG_000051"
     CDS             complement(59214..59867)
                     /locus_tag="ACHALG_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFN2585500.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            59999..60841
                     /locus_tag="ACHALG_000052"
     CDS             59999..60841
                     /locus_tag="ACHALG_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFN2585501.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            60999..61895
                     /locus_tag="ACHALG_000053"
     CDS             60999..61895
                     /locus_tag="ACHALG_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFN2585502.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            61898..62611
                     /locus_tag="ACHALG_000054"
     CDS             61898..62611
                     /locus_tag="ACHALG_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HFN2585503.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            62631..63149
                     /gene="msrA"
                     /locus_tag="ACHALG_000055"
     CDS             62631..63149
                     /gene="msrA"
                     /locus_tag="ACHALG_000055"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFN2585504.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            63146..63373
                     /locus_tag="ACHALG_000056"
     CDS             63146..63373
                     /locus_tag="ACHALG_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HFN2585505.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            63525..64076
                     /gene="lepB"
                     /locus_tag="ACHALG_000057"
     CDS             63525..64076
                     /gene="lepB"
                     /locus_tag="ACHALG_000057"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFN2585506.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(64159..64701)
                     /locus_tag="ACHALG_000058"
     CDS             complement(64159..64701)
                     /locus_tag="ACHALG_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFN2585507.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(64938..65336)
                     /locus_tag="ACHALG_000059"
     CDS             complement(64938..65336)
                     /locus_tag="ACHALG_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFN2585508.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            65608..66567
                     /locus_tag="ACHALG_000060"
     CDS             65608..66567
                     /locus_tag="ACHALG_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFN2585509.1"
                     /translation="MTYTHLTTDELVMIESYFKINQSVAKTAHFLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIR
                     CSARTIYRMFKKGLFNPSDLPMKGKRKPNGHQERRGKQAFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIENKLNHWFESVPKNLFK
                     SITFDCGKEFSNWKQISNANDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDESFIQSVASKRNNIPRKSLNYRTPLEVFLSYVSIDDLSNLI"
     gene            66661..67521
                     /gene="ylqF"
                     /locus_tag="ACHALG_000061"
     CDS             66661..67521
                     /gene="ylqF"
                     /locus_tag="ACHALG_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFN2585510.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            67514..68281
                     /locus_tag="ACHALG_000062"
     CDS             67514..68281
                     /locus_tag="ACHALG_000062"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFN2585511.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            68337..69194
                     /gene="dprA"
                     /locus_tag="ACHALG_000063"
     CDS             68337..69194
                     /gene="dprA"
                     /locus_tag="ACHALG_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFN2585512.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            69316..71394
                     /gene="topA"
                     /locus_tag="ACHALG_000064"
     CDS             69316..71394
                     /gene="topA"
                     /locus_tag="ACHALG_000064"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFN2585513.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHGHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            71363..72739
                     /gene="trmFO"
                     /locus_tag="ACHALG_000065"
     CDS             71363..72739
                     /gene="trmFO"
                     /locus_tag="ACHALG_000065"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047151 - tRNA
                     (uracil(54)-C5)-methyltransferase activity,
                     5,10-methylenetetrahydrofolate-dependent [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HFN2585514.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            72937..73842
                     /gene="xerC"
                     /locus_tag="ACHALG_000066"
     CDS             72937..73842
                     /gene="xerC"
                     /locus_tag="ACHALG_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFN2585515.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            73879..74427
                     /gene="hslV"
                     /locus_tag="ACHALG_000067"
     CDS             73879..74427
                     /gene="hslV"
                     /locus_tag="ACHALG_000067"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslVU peptidase proteolytic subunit"
                     /protein_id="HFN2585516.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            74441..75841
                     /gene="hslU"
                     /locus_tag="ACHALG_000068"
     CDS             74441..75841
                     /gene="hslU"
                     /locus_tag="ACHALG_000068"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HFN2585517.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            75859..76650
                     /gene="codY"
                     /locus_tag="ACHALG_000069"
     CDS             75859..76650
                     /gene="codY"
                     /locus_tag="ACHALG_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HFN2585518.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            76732..77607
                     /locus_tag="ACHALG_000070"
     CDS             76732..77607
                     /locus_tag="ACHALG_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFN2585519.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(77739..78377)
                     /gene="plsY"
                     /locus_tag="ACHALG_000071"
     CDS             complement(77739..78377)
                     /gene="plsY"
                     /locus_tag="ACHALG_000071"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFN2585520.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            78504..78938
                     /locus_tag="ACHALG_000072"
     CDS             78504..78938
                     /locus_tag="ACHALG_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFN2585521.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            79104..81149
                     /gene="parE"
                     /locus_tag="ACHALG_000073"
     CDS             79104..81149
                     /gene="parE"
                     /locus_tag="ACHALG_000073"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFN2585522.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            81161..83611
                     /gene="parC"
                     /locus_tag="ACHALG_000074"
     CDS             81161..83611
                     /gene="parC"
                     /locus_tag="ACHALG_000074"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFN2585523.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            83888..86119
                     /gene="pflB"
                     /locus_tag="ACHALG_000075"
     CDS             83888..86119
                     /gene="pflB"
                     /locus_tag="ACHALG_000075"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFN2585524.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            86363..87124
                     /gene="pflA"
                     /locus_tag="ACHALG_000076"
     CDS             86363..87124
                     /gene="pflA"
                     /locus_tag="ACHALG_000076"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HFN2585525.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            87209..88135
                     /locus_tag="ACHALG_000077"
     CDS             87209..88135
                     /locus_tag="ACHALG_000077"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HFN2585526.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            88317..89210
                     /locus_tag="ACHALG_000078"
     CDS             88317..89210
                     /locus_tag="ACHALG_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HFN2585527.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            89344..89469
                     /locus_tag="ACHALG_000079"
     CDS             89344..89469
                     /locus_tag="ACHALG_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFN2585528.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(89603..90235)
                     /locus_tag="ACHALG_000080"
     CDS             complement(89603..90235)
                     /locus_tag="ACHALG_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2585529.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(90383..90925)
                     /locus_tag="ACHALG_000081"
     CDS             complement(90383..90925)
                     /locus_tag="ACHALG_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HFN2585530.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            91060..91449
                     /locus_tag="ACHALG_000082"
     CDS             91060..91449
                     /locus_tag="ACHALG_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2585531.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            91453..92217
                     /locus_tag="ACHALG_000083"
     CDS             91453..92217
                     /locus_tag="ACHALG_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFN2585532.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            92346..93294
                     /locus_tag="ACHALG_000084"
                     /pseudo
     CDS             92346..93294
                     /locus_tag="ACHALG_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
BASE COUNT        31754 a        16090 c        19583 g        25886 t
ORIGIN      
        1 atttttgtca cagtcccttt aatcttagta tatttttctc tacttgaata aaaaaattat
       61 aggattggag aaaataatcg agaaatttgt tgcttgaata atagccagtt agacatttcc
      121 ttgactgttt cagttgtcat ggatgaacaa tgtgtctcat cttctttgaa ctgagtagca
      181 agttcttcta aaaactcagg atcgtaaatg acagcacttg tttcaaaatt caatcggtag
      241 ctacgaatat cttgatttgc tgaacctacc atgcagattt catcatccat tatcaatgtt
      301 tttgcatgga ggaagccgtt ctcatagaca aggatttgta cattttcctt gatcagctgc
      361 cgagcataat attgtgttgc tcgataaata aaaggatgat ccggtttgtt gggaatcatg
      421 attttcacat ctacaccaga ggcagttgcg atttttaaag cagcaatgac actttcatca
      481 ggaacaaggt aaggtgtctg tatccaaaca cgttttttag cagaagtaat caatttgata
      541 aatgaaagct tgatttgttc cctttcgtta ttcggtccgc ttgaaacaag ctggatggat
      601 gtatcagcaa gcttagattc atctcgatct gcttttttaa agaaataatt caaatgatag
      661 ccgacctttt tttcttcagg taccgagacg ttccagtctg ttaagaagcg aagctggagc
      721 aaagaagaag cggcaccgaa aatccgtata tgcgtatcgc gccaatagcc gaattttttt
      781 gttgtattta catattgatt ggcaatattg aaaccaccgg tataactaat ctttccatcg
      841 atcacaacga tttttcggtg atcatggtaa ttcaatcgga aacgaagtaa tgccttttgt
      901 gaggtaacaa atgtatggac atgtccacca tttttgatta gacgattgaa atcttttgct
      961 tttgtaccat gagagccaaa tgcatcatat aatattcgaa cttccacgcc ttctgcggct
     1021 ttttcttcta aaacatgtaa gacttgctgg ccgatattat ctgtcacaaa tgcgtaatat
     1081 tcgatatgga tcgaatgttt ggcttttttt agatcttgaa gcagtgaatc caatttctct
     1141 tgtccgtctg tgagaagagt gacagaattc attcttgtca gcggcatacg atttaatgaa
     1201 gagaagaagt cgacaaattg ctgtttgtct ttatcgccca tgtcaggatc gtaatgttca
     1261 atggtatctc ctctaaaaga ctgaaagttt tctaattctt tcaagtcact ttgtcggaga
     1321 tagaattttt tcttatccgt taatccacgt ccgaaaaata aatataatac aaagcccacc
     1381 ccaggaaggg caaacaatac taagagccat gcccaaatgg ctgctacatc tcggggtttg
     1441 atcaaaatag ccaccaaagc aataagtgca tttaatagat aaagggcggt tataatacta
     1501 gataccacaa aaatgttcct ccttacatac aataacatta gtatagcatg atagaagaaa
     1561 gaagcctacc aaggtgaata tacttttcac tcctttcatg aaaatgtagg taaatcgggc
     1621 taaagtagtt gctttttgta ggttaataag gtaggatatt agtacagttg aatatctcct
     1681 taggggagta accggcaacg tttgttgcga taatatcaac agtaagttaa ccaaatggtt
     1741 ttctggtatt gtatgaaatg gtgagaccta agacaaatgc gtatttgtct taggtctctt
     1801 tttttttcag acaggactag ggccaagatc aaaaaatggg ggatgaaatt tggaaaacaa
     1861 ggaacaagca caacaagcgg tctataaaga aacgatcgac tcgttgatgc agcaaacgca
     1921 gagctcaacc gatggattga gcaccacgca agcgaaggaa aaattagaaa gagatggctt
     1981 gaaccagcta aaagaaaagc cgaagaagcc aacttggaaa ttgttcttag aaactttcaa
     2041 agatgcaatg gttatcgtat tgctgattgt cgcactgatt cagatggtca tgggtgacta
     2101 catcgaattc ttagttattt ttgcagtatt gatgttgaat tctataatca gtgttgtcca
     2161 aacaaaaaaa gcagaaggtt ctttagaagc gttgaaaaac ctttcagcgc ccgatgctcg
     2221 agtgattcgt gatggtaagg aaaaaacgat tccggcaaac gaacttgtgg taggagatat
     2281 cgtgttgtta gacgcaggcg attatgtacc tgctgatgga cgtttactag atgcgggatc
     2341 attaaaagta gatgaaggta tgctgacagg agagtcgact tctgcagaaa aagaagtaac
     2401 ggatatcaca agtacggtac caatcggaga tcgtacaaat atggtattca gtggaacgat
     2461 cgtaacttac ggacgcggta aattcttagt gacttcaact gggaataata cggagatcgg
     2521 acaagttgct ggccttttgg aatcaacaac tgagcaagtc actccgttac aacgaggtct
     2581 ggataaattc agtaaaaaac taagtctggc tattttggca ttgtctttat tgattttagg
     2641 tatccaactg tttcgtattt atcttggaga aggtacagga gatatgactg cagatatcgt
     2701 cagcgcagtg atgtttgccg tagccgtagc cgtagctgct attccagaag cattacagtc
     2761 tatcgtaaca atcgtgttat ctttgggaac gaataaaatg gcaaaacgtc atgccattat
     2821 tcggaagctt ccagcagttg aaacacttgg ttcgacaagt atcatctgta cagataagac
     2881 tggtacattg acacaaaaca aaatgacagt tgttgactac tttttaggta atggcgatac
     2941 tggggatttc acaaatgacc cttcaaaatg gacagctgac gaacgtcgct tgatcgaaat
     3001 cagtgtttta gcgaatgatg catcgatcag tgaagacggt actaaattgg gcgaccctac
     3061 ggaagtagcg tttatcgact ttagtgaaaa attaaaccag ccttatcagg aaatccgaaa
     3121 caactaccca agacaagcag aacttccttt cgattctgat cgaaaattaa tgtctactgg
     3181 acacacgatc gataacgaaa attacttgtt tgtcaaaggc ggaccagata tcgtcttcca
     3241 acgcagtaaa aaagtattgc taaatggtga agtcgttccg atgacggatg aattgatgaa
     3301 aaaattccaa gagcaaaatg aagctttttc aacacgcgca ttacgtgtac tagcttttgc
     3361 ttaccgtcca cttgataatt ttgatttatc atttgaagat gaagacgatt tgatccttgt
     3421 tggtttgctt gctatgatcg atcctccacg tgctgaagtg actcaggcgg taacagaagc
     3481 aagaagtgca ggaatcaaaa caatcatgat tactggtgac cacaaaacaa cagctgttgc
     3541 tatcgcaaaa caaatcggga tttttgaaga aggaaacctg gctttgaccg ggacggaact
     3601 agatgaattg acagaagatc aactaaacga agttttagaa aaagtcactg tttatgcacg
     3661 tgtttcacct gagaataaaa ttcgtatcgt tcgtgcttgg caaaataaag gtcatatctc
     3721 agctatgaca ggtgatggtg taaatgacgc accagcattg aaacaagcag acatcggaat
     3781 tgctatggga acgggtacag atgtggcaaa agatgcagca gcgatggtat tgacggatga
     3841 taactttgct tctattgtca gcgcagtaga agtaggacgt aatgtgtttg ataatatcaa
     3901 gaaagctatt tcttatctat ttgcaggaaa tttaggtgcg atcattgcga tcatctttgc
     3961 cttagttgct gactggtcca atccatttac agcattacaa ctgttgttca tcaatttagt
     4021 caatgattca ctgccagcaa ttgcattagg tacagaaaaa gctgaacctg ctacgatgaa
     4081 ccgcccgcca agagatccaa attcaggaat ctttacggac aaaacattga tttctgtaac
     4141 gtatcgtggt gtgctgattg ggattgctgt tatctttgct caatggcttg gcatgcagca
     4201 ttcacctgaa ttcggtgtag ccatgtcatt ttcaacattg atttggtgtc ggacattgca
     4261 aacattccca gcacgttcaa acacacaaac agctatccaa gcagggttct tctctaacag
     4321 aagtgtctta ctagctgtag ttgtatgtag cgcgctttac tgcttaacaa tgatcccagg
     4381 actgaaagaa gtcttctcta tcccatcagc atttggattg acagaagtct ttacatgtat
     4441 gggaatcgca cttgtgacaa ttgttttgat ggaaatcact aaattgatca ttgtacgagt
     4501 acaaggaaat aaataaaacg aatcaacgag gttgtgagcc tgccgtttaa ttctgagtca
     4561 caaggtgcaa tttcaacaaa cagcttctca tgtttgttga atattgtgac ttgtgaccga
     4621 agagttggca ggagaacacc gtttaaggaa gaagttgtaa aatcagcatt ttgagctaat
     4681 actgagggtc atctgattgg acactgttta ttcggaaaga gggactttga cacgactttg
     4741 gtcacagtcc ctttttttat ttatgataga cggaaagcca atcattttat tattcatcaa
     4801 aagagataga atagataaaa gaggagaata atttattccg aaaagtgaaa acatataaca
     4861 aaaaattaga ggaaatcaaa tgaatgaaaa agaatgggat gaatttgctg aagaatatta
     4921 tgaaatccag caagaatcgc aagtaactat tgctgaagat gtaaaaaatt atctgaaaac
     4981 ggagaaagtc ctgcctgctg aaacagcagc agacgttgct ggaggatctg gcagatttct
     5041 ttccttagca aaagaaatta atcgctatga tttgatcgat ttttcttcag gaatgctaga
     5101 aaatgcaaaa aaagaagcag aaaaaaatga gttgccaaat gtttccttta tcaaacagtc
     5161 gttagaagaa tttatccaat caaatgagac atatgaaatg gtttttacag cagcaaatcc
     5221 agcattggta catcttattc aattagaaaa attgatgaaa aagtcaagca aatggtgcgt
     5281 gataatcagg gtagtggaat caatcgatga tgttttttat cctatcgaaa agcaactagg
     5341 aatgatagaa gaagatccta atacatcgcc agttgtaatg gatcaatttg aaacatactt
     5401 aattgatgaa ggattcaaag tgaagaaaaa agattttact tattttacac aggaatcaat
     5461 tactgaagaa ttgatgaagc tatactatga cgcgtacgca aaagatcctg ttgtgaccag
     5521 gtacattcgc aaactattca gtgaaaaacg agaaatcctt agtacgacta agctgaccta
     5581 tagactgctc ctttggtccg cagataaaaa aagaaccttt cacgactcgt gaagggttct
     5641 ttttatgatt ttattatatt ttgtagtaac gaatatcatt ttcgatcttt gtaatcgaaa
     5701 aaggtacatc tgaagtatat tctgcacatt ctccgtgttc aggaagcgtc tcactgaaaa
     5761 tcgtttcata ttcgtcaaca gtgatccgtg ttcgagaatc tagcatctct tgatgagatt
     5821 gtgcgaagag atattcttga tagttttctt ccagataacc tgtaaaaaat tcacttactg
     5881 caccagaacc gtagctgaat agaccgatgc gatctcctgg ctgtagtgat ttcgaatttt
     5941 ccagtagaga agttagcccc aggtataatg aaccagtgta aagattaccg attcgtcggc
     6001 tgtaacggat gctttcttca tagcgagcca taagacgttc ctgattttct tcgtctgttt
     6061 ggtctaatac gctttgcaat gccttttttc ccatcttagt atacggaata tggaaagcaa
     6121 tcgcctgata atcttcgagt cctcgacccg acaattcttt atgtcgattc caaacttttt
     6181 ggaatgattc gatatacgta gaattagata aaggaccatc aacaacagga aattcgctat
     6241 aatctggacg ccagaaatca tagatatctt ctgtcagaaa tacgctgtcg tcttcaatcg
     6301 ataaaatacg cgggttttga gtgatcatca tcgcaacagc accgacacct tgcgtcactt
     6361 caccaccgct tgccaagccg taacgagcaa tatcacttgc tatgactagt acttttcgtt
     6421 ctggatgatt tttgacatat tctttcgcca tgtgcaaagc tgcggtgcca ccgtagcaag
     6481 cctcttttac ttcaaaagaa cgagcaaaag attggatttt cagtaaatgg tggataatta
     6541 ccgcactcgc ttttgaatga tcaatcccgg attctgttcc gacgatgacc atgtcgatta
     6601 gctcacggtc tttttctgtt acgattttgc tggcagcatt tgctcctagt gtaatgatgt
     6661 cttcatttgc acgattgact gccatttggt cttgaccaat ccctatatgg tattttgcag
     6721 gatcatcccc acggctttct gccaactctg tcatatctaa atataaatta ggaataaaaa
     6781 aggaaagacg atcaatccct attttcattt caacacctct ttacttatga taaaaaactt
     6841 actttactaa ggtatcataa aaagattaaa agaaatataa aaaagagaat atttaaggat
     6901 tgttaaaatt taatcttaga aaaaaacaac tggaacaaga gctatttgtt tttacagcaa
     6961 agaatggtat atttttgtag gaggtggctt acttgaaaga agtcgttatg atagatgctg
     7021 caagaacgcc gataggaaag tatcgcggaa gtttaagtcc atttactgca gttgaattag
     7081 gcacacttgt gactaaagga ttattagata aaacaaaact gaaaaaagat aaaatcgatc
     7141 aagtaatatt cggaaatgtc ctgcaagctg gtaatggtca aaatgttgcc agacagattg
     7201 ctttgaacag cggtctccct gtagatgtcc cagcaatgac gatcaatgaa gtttgcggat
     7261 ctggtatgaa agcagtgatc ttagcacgcc agctgatcca attaggtgaa gcagaacttg
     7321 tgatagccgg cggaacagag agtatgtctc aagcacctat gctgaaaccg tatcagtcag
     7381 aaacaaatga atatggtgaa ccaatttcca gtatggtcaa cgacggattg actgacgcat
     7441 tttcaaatgc acatatggga ttaaccgcag agaaggttgc aacacaattt tctgtgagca
     7501 gagaagaaca ggatcgctat gccttgtcgt cccagttgaa agcagcacat gctgtcgaag
     7561 ccggtgtatt ttctgaggag atcatcccag tcaagatttc tgatgaagac gtgttatctg
     7621 aggatgaagc agttcgtgga aatagtacat tggaaaaact gggcacgtta cgtacagtat
     7681 tctcagaaga aggaactgta acagcaggaa atgcttcgcc attgaatgac ggtgcctctg
     7741 tggtgatcct tgcatccaaa gaatacgcag aaaataataa tctgccttat ttagcaacca
     7801 tcaaagaagt agcggaggtc ggtattgacc caagtatcat gggaatcgct ccaatcaaag
     7861 ccattcaaaa attgacagat agatcaggaa tgaatttatc aacaatcgat ctttttgaga
     7921 tcaatgaagc atttgcagct tcttctatcg tagttagcca agaactgcag ttagatgaag
     7981 aaaaggtcaa tatatatgga ggagcaatcg cattaggcca tccaattggt gcaagcggtg
     8041 cacgtatctt gactacatta gcttacggat tgcttcgtga acagaagaga tacggtattg
     8101 cttcattatg tattggtgga ggtttaggct tggcagtctt gttagaagcg aatatggagc
     8161 aaacacataa ggatgtgcaa aaaaaaaagt tttatcagct tacgccatct gaacggcgca
     8221 gtcaattaat agaaaaaaat gtattgacac aagaaactgc gttgattttt caggaacaaa
     8281 cactttctga agagctctcc gatcacatga tcgaaaacca agtgagtgaa gtggaaatcc
     8341 caatgggcat cgcgcagaat tttcaaatca atggtaagaa aaaatggatt ccgatggcaa
     8401 cagaggaacc atctgtcatt gcagctgcaa gtaatggtgc aaaaatctgt ggaaatatct
     8461 gcgcagaaac acctcagcgg ctgatgcgag ggcaaatcgt tctatcagga aaatcggagt
     8521 atcaagcagt gatcaatgca gttaatcatc gaaaagaaga gctgattctt tgtgcaaatg
     8581 aaagttatcc ttcaatcgtg aaacgcgggg gcggtgttca agatatctct actcgggaat
     8641 tcatggggag tttccatgca tacttatcta ttgatttttt agtggatgta aaagatgcaa
     8701 tgggcgctaa catgatcaac tcgattcttg agagtgtggc aaacaagtta cgggagtggt
     8761 tccctgaaga agagattcta ttcagtattt taagtaattt tgctacagaa tcattggctt
     8821 ctgcctgttg tgaaatccca ttcgaacgct tagggagaaa caaagaaatc ggtgaacaaa
     8881 ttgccaaaaa aatccaacag gcaggggaat atgcgaagct tgacccttac cgtgcagcaa
     8941 cgcacaacaa agggatcatg aatggtatag aagctgtagt ggcagctaca ggaaatgata
     9001 cacgtgcggt tagcgcttcg attcacgcct acgcggcaag aaatggatta taccaaggat
     9061 taacagactg gcagatcaaa ggggataaac tagttggaaa actaacggtg cctttggctg
     9121 ttgccacagt aggcggagct tccaatatcc tgccaaaagc aaaagcatct ttagctatgt
     9181 tagatattga ttcagccaag gaactagcac aagtaattgc tgcagtaggg ttagctcaaa
     9241 acctggctgc tttgcgtgca ttagttacag aaggaatcca aaaaggccac atgggacttc
     9301 aagctcgttc attagcgatc tcaattgggg ctattggtga ggaaatcgag caagttgcga
     9361 aaaaattacg tgaagcagaa aagatgaacc agcagacagc tatacaaata ttggaaaaaa
     9421 tccgggaaaa ataaaaaagc aatatcttta gaagaataaa gcaagattta cagttatttt
     9481 atttactgca aatcttgctt ttttctctat cagaaaatat gtttattatt agaatttttt
     9541 atagttaagg ttaggcatct taagaaatca ggcaactcca agatatcttc aggtagaaga
     9601 gaagtcgcct aacattgtag gatcataggt gatttgttgg acaggtcaag ctgatatttg
     9661 agcctgatgt tagaaggaat ttctttttac ttgctgtaga aattattata tgactaaaaa
     9721 ggctgggatt ttaaatccca gccttttatg acaaatagaa tttttatcga tcagtaatcg
     9781 ctgtcgctgc aaattgttcg atcaaaggaa tcaaagtcaa tttgactttg tatttccgtt
     9841 cgagtgcttt ttcgataacg aactcatttt cagtagtcat agaaatagtt gctaatttac
     9901 gaggttcatc agcatttttt tcattaataa ctacttcatg ttcattttgt tctgtgtaaa
     9961 atttaggata ctcagcaacg gcttttgaaa aatcagcaaa acttaaagcg gcattcgtat
    10021 cagttgagcg tgtattttca gttgtgtccg cttttggttt ttccgttttt tcttctggca
    10081 cactgactgt ttcttctgta actggtgtcg caacagcttg cgcagttggt tcatcgtttt
    10141 tcaatcgttg atcattccag taagacacga tttcaataag aagattgtca cggaatcgtt
    10201 cgaaaatccc gttttctcct gaacgaattc tctttaatac agtattgaag ttgtcatcga
    10261 ttggcacgtt gatccgtgta tcttcatttg ttttcccagc taatccgatc gttaagattt
    10321 gacggttttt gggatcaagc atgacttgta acggcaaacc atagcgtcgc aagctctctt
    10381 cttctagaga acggaataat tgtcctctta caccatccaa tgaaggacgg aattgattta
    10441 ataatttttg tttctgtttt ttcttcatat gttctctcct tcgctcatac cctttcgatt
    10501 ataagaaaaa aacagccaaa tgaaaaggag taatgcaaaa aaacttgcgt atacgcaaat
    10561 tacaacagaa aaatttgcgt attttcccgt tttttgaaag aaaaataaaa aatggaacgg
    10621 attatgtata tatacataat ccgttccatt ctttctattt tttcggtatc aaggcaaaaa
    10681 caggtagttc aggtgaataa atgacttcat gttcgtgcca gggtttgacc attagcgaat
    10741 gatcgaaccg ttttttcagt tcttcgatga tttgatcatg tgtccagttt cttgcaggaa
    10801 aatgttggat tttctctgca tcgatcaaaa agacaaaatc ggaatgaaaa gtctgggtaa
    10861 cttcttccga gagagaattc aagcgttcga ttggatccat aaaatcacct ttcgacttaa
    10921 aattgggtag tatctgggtc ttttgctttg ccgactacac catctaactg atcgatttgt
    10981 ttcatttctt cttctgttaa ttcaaaatca aataattcag tattttcttt gattctgctt
    11041 ggtgtcactg acttaggaag aggaaggaat tcgtgttgca gcgaccaacg caaagcaact
    11101 tgtgcaatcg tcttgtcatg tgcttcagcg atttgtttca tttcaggcac atcgaagatt
    11161 ttaccagtgc ctaatggact atatgcttcg aggatcatat catgtttttt tgcatactca
    11221 actacagttg gctgtaattc gcctggtgca aggaaaactt gattgaccat cggcataatt
    11281 tttgctgttt ttgctagtgt atctagatgg tggggtaaga aattgctcac accgattgct
    11341 ttgattttac ctgcttcata cagttcttca aatgctcgcc atgtatctgc atttgcttgt
    11401 tcccaattat cacgaaaagc aacagggttt ggccagtgga tcaaaaacag atctaaataa
    11461 tctgtttgga gcttcttcaa tgattcttca aaagaagaca tcacaagttc atagctgtgg
    11521 tttgcattcc ataattttgt tgtcaagaat atctcttctc taggaattcc actttcctta
    11581 atcgcttggc cgacactttc ttcattcttg tatccttggg cagtatcgat atggcgataa
    11641 cctgcagcca gcgcttcttt aacagaggaa acagcgacat ccccatcagg tgtttgccat
    11701 gtgccgaatc ctactaccgg gatttcgtaa ccatttaata gacgataagt gcttgttaat
    11761 gaattcatac tttcagctcc ttttttattc gttcaactat aagtgtagcg catttttcga
    11821 aaaaaagaat ccattttgtt tctactcttg aaatttttgt ttaaaagaaa atgattttga
    11881 ttctggtatt tttgattttt tttccgtaaa ctaagacaga agggaatgag aaaatggata
    11941 tctatttaaa aaaagcaatt ctgcatatta ttgacagaga aactggttct ccagtctttt
    12001 cacaaaaaga gctggatctg acaaaagaat atattcgtga ttttcttcaa aagaagatcc
    12061 aaaaaatctc ttctgctcaa acaaaaactg gacaactagc agaagaaagc atgatcgttg
    12121 aatcacttaa aatcgctcaa gaaaattttg tggaagcaag tgaagcaatc gttcagcggt
    12181 ggttcgatat ctaccaagaa agtgaagaag cacctagtgc agatgtattt gttgttttgt
    12241 atgaattaga tacagtgatg tacttatctt tattgaaagt caattatcgt gaggcttaca
    12301 ctcactttgt tgaagcagac gaagcaggga tcgacaaccg cttgattttg aatcgagcaa
    12361 ttctaggggc gaaaacacaa aaagcagatg aagcattcgc tgtgaatttg actgattgga
    12421 cctacgaatt aattgaaaaa aaatatgagt tttctggcaa aaaagagctt tatttttctg
    12481 gaagagtgat cgaaagtgtt cctgcacctt ctttagaaga aaatgtaaaa gtgatccaaa
    12541 aagtagccaa gcatcttgga aaaaaatttg agacagaaga atttgatatc atggctgatt
    12601 tgaaagaagc tgtctatgat acgatagaag aaaaaggtcg gttggatcat gagatgattg
    12661 cggaaaaagt cttcaaggaa aacatcacgg caaaaatggc ttttaaagaa gaagtacaag
    12721 agcaaggatt tgtcccagaa gcacctcctg taaaagaagt acaagaaatt tctgagaaaa
    12781 aatttggaaa acaaaaattc aagctatcaa acgggatcga actgatcgta ccagtagatg
    12841 tatatcgcaa cccagatttg attgaatttg tgaataatcc ggatggtacg atatccgtga
    12901 tgatcaaaaa tgtggatgaa gtattgaatc gtttataaaa atgactagaa cattaggtgt
    12961 tcaagtacca gtgatcagtt aaatgaattg tgtcaagaga aggatcggaa aagacgaata
    13021 taacaaaaaa agagcgtgag acaaaagtaa agaacacttt tgtctcacgc tctaaacacg
    13081 tataaacggc ggaagcagaa gcaactcctt cttatttctt ggagttaaac gcttctgtcc
    13141 cagcctcttt ttatctaata aaattttaag accatttact aattatcgat tttttctgcc
    13201 gcatttttag gcacttcaga agcctttatt tcttcccagc ttaatacacc tttacgagga
    13261 ttgactttga gtttaagata cgcattcatc tttaatggat gttctctttc ttcgcgaagc
    13321 gtcacctgtt ttttttcacc atctttatta taagccgctt gcttgtaggt ataagtcgtg
    13381 aatttctctc cgttatctgc ttgggtagct gacatttcgc cattcgtcgt gatttttgta
    13441 taataagctt cgccgccgta aaaataatga taagctgcaa aacttccgcc gagaaatatg
    13501 cctaaaacga caagtacgcc aattaatttt ttcatgtact tcacctcttt ccttattatt
    13561 aacgaaatag aagatttcgt ctattgaaag ttgttggcaa cagctgaatt ttaaactttt
    13621 cctcattttg ttcttattgc ttcaaaagaa agttaaggac tatcttgaag tttttctcaa
    13681 aaaacacatt tttttattta taaaaggtac ttttttcgcg tataagacaa gcgagtgctc
    13741 ttagttgact ttgaccaata ttgacgtata ataagcttag ttcgaatctt acaaaaagta
    13801 agtcggacgt attctttttg atttagtcaa aaaaagacta taataaaaag gtgagagctg
    13861 aaatttttca gtgatctata ttatttagga ggtttgtaaa ttatggcaaa agaacattat
    13921 gacagaagta aaccacacgt taacattggt acgattggac acgttgacca tgggaaaact
    13981 actttaactg ctgcgatcac tacagtatta ggcaaaaaag gcctagcaaa tcctcaagac
    14041 tatgctagta ttgatgcagc tccagaagaa cgtgaacgtg ggattacgat caataccgca
    14101 cacgtagaat atgaaacaga aaaacgccac tacgcgcata ttgatgcacc aggacacgcg
    14161 gactatgtga aaaacatgat tacaggggca gcacaaatgg atggtgcaat cttagttgtt
    14221 tctgcaactg acggtccgat gcctcaaaca cgtgaacaca ttttattgtc acgccaagtt
    14281 ggtgtaaaat acctgattgt tttcttgaac aaagttgatt tagtcgatga tgaagaattg
    14341 atcgatttgg tagaaatgga agttcgcgag ttattgagcg aatatggttt cccaggcgat
    14401 gacactcctg tgatcaaagg ttccgcatta aaagcattgc aaggcgatcc agatgctgaa
    14461 gctgctatta tggaattgat ggatacagta gatgaatata tcccaacacc agaacgtgat
    14521 acagataaac cattactatt gccagtggaa gacgtcttct caattacagg tcgaggaact
    14581 gttgcctcag gtcgtattga tcgtggtgct gttcgtgtcg gtgatgaggt agagatcgta
    14641 gggatcaaac ctgaaacaca aaaagcagtt gtaacaggtg tagaaatgtt ccgtaaaacg
    14701 ttagattacg gggaagctgg ggataacgta ggcgtgttgt tacgggggat ccaacgtgac
    14761 gatatcgaac gtggacaagt gcttgctaaa ccaggttcca ttactccaca tacaaaattc
    14821 aaagcagaag tgtacgtgtt gacaaaagaa gaaggtggac gtcatactcc attcttcaac
    14881 aactatcgtc cacaattcta cttccgcaca actgatgtta caggaacaat cacattgcca
    14941 gaagatacag aaatggtcat gcctggtgac aacgtgacga ttgatgttga cttgatccat
    15001 ccaatcgctg ttgaaaacgg aacgacattc tcaattcgtg aaggcggacg gacagtgggt
    15061 tcaggtatcg ttacagagat cgaagcataa gaaacgcgac tcgaatattt tggtaatgaa
    15121 aaatgtttaa aataaaagag cggtccaaac aggatttctg tttggaccgc tctttgttct
    15181 tcaattgtat aagaagagat tgtagaagca tgaactttcg ttttacttca gtctctttta
    15241 ttcatcatga gagtgttcat ctatcggtat gcagttttta atactttctt cgtatccatt
    15301 ttttcaaacc acttattcta ccttgtgtct gctcaactgg cgcattatca ataatcagca
    15361 aagcaatcat aacatatgca atccaactat ctctagaata tagagtataa cgtgagaccc
    15421 agtttgtagc gtacttgctt ttatattctc ttaatccatg gaaagaataa aaatgagaac
    15481 caaactcgta aaccaagtat gcaatccttt cctgtaaaaa actctttctg gaagttccga
    15541 catttgaaag aggggccatc cctaaattga accattggat atgttcttct ttcatatagg
    15601 caaacaaatg caagaacaag aaatccatac tgccagaagg tgctttttta ggatcatatc
    15661 gcatcagatc aatcgttcct actttattgt tcgtgtaagt aggcatgatc gttgcaaaag
    15721 cgacaatttc ttcttgggta tttttgacaa ctgcaatagg cgctctttgt agataatctt
    15781 cagagaaaaa gcctaaagaa aacccttttt cctttcgtga acccaaccaa ttatcagaga
    15841 tatttttgaa agtactcatt tgctcagtag aaaatggtgg ttgtaagaca tcaaatgtaa
    15901 atccttctcg ttcgatccga ttcaagacag cacgagtgcc cttcattttt ttccctgaag
    15961 ttgtaaaact attcaggtct acataagctt cttcccccat cttaatgaaa tcgtagccga
    16021 attcatgcaa aatcataaca atctcttcgc ttgtttcgta gaaaactggt aaatagcata
    16081 gtcgatctgt ttcttcgata aaagcttcga ttgcttctgg aaaatcttct ttcttaccag
    16141 aaggatcacc cattacaata catttattat tgaagcaagc gaattggaga agaacagttg
    16201 gttcaccgtc tttttcatag gcaaacatcc gcttatcttt taagaagatc aattgactat
    16261 cggagtttcc tccataagtt gtaaggatct tcagggcctt ttcttcatta aaggcttcgc
    16321 caatctgttt cttttctcct tgaaggaaat gaacaaatag aacgatcatg aaactaacag
    16381 cgatgatggc caacaatccc gaaaaccaaa tcttttcaga aggaaataag aaaaaggaaa
    16441 taaaatggtg acgccgatga gggaaatccg gcagattgta taccccaata acgatgtaaa
    16501 gcaaggacag tgcaccaata atgattccat cgatcgtacg ccactcccat gaatagatca
    16561 attgttcccg aaataattcg tttttagaag caagaatgat cagaagcaga atagacaaga
    16621 aaaccaccgg tgtgaaacta aaatcactta ataaaacata aagtaaagct agaacaatca
    16681 agaagattgt aggcaaatat gctcgtttca cacgtgctgc gatccctctg cccataatga
    16741 ttaaaagaaa tcccaatagt atactaggaa attggataat aaaatgaaat ttcaatgggt
    16801 tcaaactatg aagccagcga aactctgtaa aagcttgcgg gatagtggca gaaagtacta
    16861 gcatgattcc agaaaagtat aataagacaa ccacgatttt atgcgcaatt tctgttgcta
    16921 attgtttagg gataccagaa taacgctgat caaaagtgga accgatattt ttgaagaaaa
    16981 agacgattcc aatcaaaaac ggaatgatgt agtagaacag gcgatagagc aagatccaca
    17041 caacgactat ttctcttggc acaccaatag cggacaaccc gataatcatc attacatcga
    17101 aactcccaat ttctcctgga atcatagaca cgattcctat cactgaagca gcaatgaata
    17161 acggaattgt ttgccacaga tcaatccgga tttccattaa ataaccaaca gaaaggaaac
    17221 taaacaggac accggtccat tccaacaatg aaactagcaa tagctgtcct tgagttttaa
    17281 gtgaggtacc accaaggaat cctttctttt tgaaagtagt aaataaaaag acgacaggaa
    17341 aatatagacc gccgccgatc aaccatatcc aatattgctt caaaaaagga gcaactggcg
    17401 tgaagactac aagtaaaaaa gaaagcagac tgtaaataga aagtccagcc atcaaaaaca
    17461 agaaaacttt cgataatgct tggatcacgt ttctgctctc tttcttttgt ccatatagtt
    17521 cagagcgaag ccccacacta acaaacccgc cgaaaccggc aatattgttg attgtattga
    17581 tcagccagct tgtctcaaac aagtaacgag gattctgctt ttgttcaagc atccgattca
    17641 aaataatatc ataaccaatc ataggtaaca cagagacaag accaatcaac agcataagtc
    17701 cgcttttcca tacaggtata gtggcaaacg tttctcctaa ttgctggata gatagagttt
    17761 taccgatgga catcaattca ccgacaataa tgacaatgac agaaatcaaa aagattgttt
    17821 tgaacagtcc taggtggttt ttcagccatt gtatggtatt ttttaacaaa taaatcacct
    17881 tctattccta atagttatgt ttcagaaaga aattgaagaa cttctcgatt gaagcgttca
    17941 ggatcttttc tagctaattc atgtccttgt tctttcacaa gcacaaagga agcttttggt
    18001 atagttttag ctatatacaa agaatgtttt agcttgatta catctttttt tcccacgatg
    18061 atcaaagtgg gggagttgat tttttttaaa tcattttctg taagcccgat gtcatgcagc
    18121 aaaagtttga taactaaaag atttttgcga agacttgggc gaaataaaga gagtaaccat
    18181 acccatgcat aatgaatatt actgatgact ctggcagaaa aacgaacgcc tttaacaagc
    18241 gtgtttccag aatttaaaat caatcgatga acttttttag ggaagctgct ggcaaatacc
    18301 agtgctagat ttgctccatc actaaatcca agaaaatctg cctgatcgat tttttccagc
    18361 aacatgatcg tattcagatc ctcggccatt aactgaaaat tcaacataga tgcttcgttt
    18421 gtcgaacgac catgtcctcg gctatctact agatacacgg tatagtagcg ttcgagtaca
    18481 gggacctgtt ctgaaaaaaa gcggccgctg ccgtcattgc catgcagtaa aaagagaggg
    18541 aagccttgac cgctcttttc atagtaaatt tcgctcccat ctggcatttt tgcatatttc
    18601 ttttcttttt tcacataatc acctactgtg taaagtataa cgattatatc agtcattggg
    18661 caattgtttt tactgaaaaa caacgatcat tttcgtaaga tgacaaaaaa tacaggaaaa
    18721 ttcatttttt cttaaggcgt acacagaaat caaggaagaa atagccaaga agataaagaa
    18781 atagtaagat aaaaaaagat gtcaaaataa ctaggtgatg aaatgaagcg aaaacgatta
    18841 tctattatcc gtattctttt ttacatgatc attctgacgg ttattggtgc ggtgttgttt
    18901 ttaggaggaa gaacttatcg ttcggtaaaa cgagtggaaa gctatcaaat agaaataaca
    18961 acagctgcaa aaaattatca tatggaaaac tacgagaatt tgatcaaggc gataatattg
    19021 accgaatcaa aaggaaaagg gacagatctc atgcggagct cggaaagtct gcatggagaa
    19081 gctgagatca ttgataatcc agctgacagt attgatcaag gtgtcaaata tttggcggaa
    19141 atgatagtag aagcaaagaa acaaggatgc gatttatgga cagctgtcca agcatataat
    19201 tttgggaaag attatattgc ctatgtaaaa aatcatggcg gagaaaacac agtagaatta
    19261 gcagaagcgt attctaaaga tgtgctgagc ccacttttag gaaatgagga agggaaacaa
    19321 taccgatatt ggcggatcca gtccttgctt tacaatggcg gctatctcta tcataatgga
    19381 ggtaacatgt tttatcctga tattgtcaaa atgaatgaaa aaataataga gacctatgaa
    19441 aaagttttta agtaaaatag caggaaatgt tcaatcatga ataaaattaa atgaaagaag
    19501 gagtgcgcaa atagtggaaa aaaggcaaaa aatggaaaat caaaaagtgg ggtacacaac
    19561 caaagactgg atgttacggt ttgtgaaggg aatgtttatc ggttcaggat ttatcttgcc
    19621 aggagtcagc ggtggtgcat tagcagctgt ctttggcatg tatgagcggc tgatttcttt
    19681 tttagcccac cttacaaaaa atttcaaaga aaatgttttg ttttttctgc ctgttggtct
    19741 aggcggagtg acaggcatct ttctactttc ttttttagtc agttttttat taggtgcgta
    19801 tgaaacaacg atattatggt tttttgtcgg atgtatcgct ggaactgttc ctgcgttatg
    19861 gaaagaagca gggaaaaaag gaagagatac ttctgatgtg atcatcatgg ttgttacttt
    19921 tgttctggct tatttgtttt tattatacgg cgctcaatta tttgacgggc aagtcgatca
    19981 gaattttgga acatggctga ttgccggcgg attgattggt ttgggtatga tcgtcccagg
    20041 attgagccct tctaattttt tggtgtatat gggattgtat aaagcgatgg ctgacggatt
    20101 taaagaagta cgacttgaag tgattgtgcc gatcggaatt ggtgggattc tttgtgttct
    20161 tggattgtca aaaatcatgg attacatttt ctcaaaggca tatagcaagc tattccattt
    20221 cattttaggt gtagtttttg cttcaacgat tatgatcatt cctaccgatt acgcgggatt
    20281 gggagttgct ggggtactag cttgtcctgt tctttttata gccggtacag cactaggttg
    20341 gtggatgagc cgtttggagg aacaatataa gtaaagatag attgataata aaaacagcac
    20401 acgggtgatt caaacaaaaa ggttggcatc ttttttgtaa aatcattcgt gtgtttttct
    20461 ataaatttta taaaaacaag ttgtttttaa tcttggaaca cgctagactt aaacacgtga
    20521 ttatttttga aagttggcgg aaaatggaga aaaagaaaat gaatggactt acacagcaag
    20581 aggtagttca gaaaatggct tctggccagc aaaatgacta ccaggaagat gcatcaaaaa
    20641 gtacaaaaca aatattcagc gataatatat tgacgttatt caattttctg aactttggta
    20701 ttgggatttg tctgttattt gtaggtgcgt attccaacat ggcgtattta gcaattattg
    20761 tcgtcaatat cgttattgga atttatcaag aaatacatgc aagaaatctt gtacggaaat
    20821 tatcgattgt ttcccaatca gaagtactag tgatccggga cggtaaagaa caatcgattt
    20881 ctactaaaga gctggtatta ggagatttgg taaaaatcgg agcaggagaa cagataccag
    20941 ccgatttaca ggttatatct ggacgggcag aagcaaatga agctcttttg acaggcgaat
    21001 ctgatttgat tgtcaaagaa actggagctg aattgctttc tggaagcttt ttgacaagcg
    21061 gttctgtcat ggctgaagtt atccgagtag gtgcagataa ttatgctgtc cgcttgacta
    21121 gcgaggcaaa gactcacaaa gctattcgtt ctgaacttgt agattcgatt cgcaaagtat
    21181 ccaaattcac tagtttcgtg attattcctt tggggatcat actatttttg gaagcactgt
    21241 tcatccgcga tgcttctatc aaaatttctg ttattgcatc tgcagcagct ttgttaggca
    21301 tgcttccaaa gggtttggta ttgctaataa gtattgcatt aacgacaggg gtaaccaaac
    21361 tggcaaaaaa acgaatcctt gttcaagaca tgtattcaat tgaaacgctg gcccatgtcg
    21421 atacattatg cttggataaa acagggacca ttactgaagg aaaaatgagt atccagcggg
    21481 tagaagcact acatccgctg tacgagaaag ccatcccaga aattatgggg acgtatctgg
    21541 cagaaagcag tgataacaac atcactatgc gggctatgag agattatttt gagctttcta
    21601 atcggtatga tgtgacaaac aaagtcgcat tttcttctga tcgcaaatgg ggcgcgatgg
    21661 aatttccaga actgggagtc gtgtacgtag gagcgcctga acgtttggtg gaagaagatc
    21721 ggcttccgga cagtctaaaa acagcccaag cgaatggcta tcgcgtactt atgttaggga
    21781 tcgctccaaa tcagcaattg aacgaagaaa aaccagcatt tgttgagccg gttgctctgt
    21841 ttgaaatcga tgatccaatc cgaaaaaacg ctcaagaaac actagcttat cttcatgagg
    21901 aaggtgtgga tctgaaagtg atttctggag acaatccttt tactgtctcc aatatcgcta
    21961 gacgtgcagg attaccgaat tatgatgcgt atgtcgatct ttctcaaatt actgaagaaa
    22021 tggaagtgcg agaagctgct catcgctata cggtttttgg acgagtgagt cctcagcaaa
    22081 agaaattgct ggtaaatgaa ttgaaagaaa gcggcagaac ggttgcaatg acaggcgacg
    22141 gtgtgaatga tgttttggcg ttaagagaag cagattgtag cattgctatg gcagaaggtg
    22201 atggagcaac acgtcagatt gcgaatctgg tattgttgga ctctgatttt actacattgc
    22261 cagaagtcct gtttgaagga cgccgagtgg tcaacaatgt gacgaaagtc tcaggcatct
    22321 tttttatcaa aacgatttat tcatttattt tatcgattat atgtgccgtt acagcaattg
    22381 gatttccgtt tatccctatc caaatcacgc tgattgattt agcgattgaa gggtatcctt
    22441 ccttcttttt atcttttgaa caagataaaa gaaaaatcac ttaccgttat ttgcctacag
    22501 cacttattaa tgctttacca aatgccttgc ttgtagtagt gaatatcatt gcggtgtatc
    22561 tacttggaaa aacattcgca tggggaaatg ctgaaactac tactttgatg tattacttgc
    22621 tgattgggat cagttgcatg gcagttgtaa aagcttgtta tcccttcaac ccattgcgca
    22681 tcttcctagc agtaacgacg atcgtaggga tatatgtagc tgcaatgctg ttccatggaa
    22741 ttttggaagt aaacttaggg ttgggagaga gcgtaggata cttctttgta ttcatgctcg
    22801 taaatattgt tcttcgagta ttgtactgga aggctgatat tcaaaatcag ttactgaaaa
    22861 gagtagcaaa ttgatgctta atgagataga aatgtgttat acttgaaaaa gcaatgaaat
    22921 atgatataat caaacatgta taattgtgtc ctttaaaatc gcactgattt cagttagtgc
    22981 gattttttac cggttattct cctgaaatat cttctgttaa acccaaaaga tatcaataga
    23041 gcagggggtg gaaaaatggc tcaacggatt cctcaagagg ttatcgaaac agtacgcagt
    23101 caaacgaata tcgttgaggt gattggacaa tatgtccagt tgaaaaagtc tggtaaaaat
    23161 tacttggggc tgtgtccttt tcatgaagaa cgtacaccgt ctttttctgt tgcagaagac
    23221 aagcagattt ttcattgctt tggttgtggt aagggcggaa acgtgtttac ttttcttcaa
    23281 gaactagaag gtctaagttt tccagaagca gtggtaaaag tggcagactt cgaacagata
    23341 ccgttagaag atcaatggcg tcagcgtact atagctgtcc aaaatcctga atctgctacg
    23401 gggaaactga ttgctgctca tgaaaaagca gcggaaatct atcatcacat gctattgcat
    23461 acaaaagctg gacagccagc tttagaatat ttgcaaagtc gtgggttgtc tttagaatta
    23521 attgaagaat tcaacatagg ttttgcacct aatgagcgtt cttttcttca gcaagtattc
    23581 aagaatgaat caatttcaaa tgaattgtta gaacgctctg gattgtttgt cacgcatgat
    23641 gatggccgta tgtctgatcg tttttatcaa agagtgatgt ttccacttcg taattctcaa
    23701 ggccagacga tcggtttttc aggacgctgg cttgcacctg aaaaagggca ggaaaaagat
    23761 cagcccaaat acttgaatag tccagaaaca gaactgttca ataaaagaca agttttattt
    23821 aatcttgata aagcaagaag cgatatccgg aaaaacggag aaattttgtt gttcgaaggg
    23881 tttatggatg tgattgctgc atggcaggct ggtatcaaaa ctggattagc ttctatgggg
    23941 acaagtctaa cagctcaaca gatccaacaa atggaaaagc tgacgaaaga attggtcttt
    24001 tgctatgacg gagacaacgc aggttttgaa gcaaccaatc gagggattga attgcttcgg
    24061 caaaacagtc gcttagcact ttctgtcgta gtcgttccag aaaagcttga tcctgatgaa
    24121 tatctccgta agtatggaga agcatctttc caagaattag tggctcatgg gcgcgaaaca
    24181 gtctttactt ttaaatcacg ttatttaaaa caagggaaaa atttagataa tgaaaaagac
    24241 aagatcgaat acctcgaaga attactagtt gaacttagcc gagttgtttc accaatcgaa
    24301 caagatatgt atctatctca actgtcaaca gagtttcaga tcggcagaga gacgatacaa
    24361 aaacaactgc gcgatcttcg aaggacaaat cagcaaaatc gtcaagaacc tcaggaaagt
    24421 caacacactc aggttacgag acaagaaact gcacggaaaa agagaccttt gacgcaagta
    24481 gaaaaggcag agcagattct attatatcgt gcatttaaag aagtttctgt ccgtaatatt
    24541 ttaaaagaac gagaatttca gtttattcat gatgcatatg cagaagttta ttttttattc
    24601 gatgcatatg tctcacaaca ttatgaattc aaccttgcag agtttttaga ctttttgcaa
    24661 gatgaaaatt taaggcggtt ggtggtggaa atcgaatatt taccggtggc agaagaaagt
    24721 tcgccacgag aaatccaaga cttgctagat gtgatcagca aatctggctt agctgatgaa
    24781 ataacattaa aaaaacaaat gcagcaagaa gccagacgaa ctggaaacaa acagcttgag
    24841 cttgaattga cgattgaaat catcaattta acgaaacagt taaaacaagc caaatagcta
    24901 ctcaaggggg gccttctttt atggcaaatg gaacagataa aaaaacgtat gaagcagcag
    24961 tagcagcttt catcaaagaa aacaaaccca aacgacaagt tgtatatgat gatttatcca
    25021 atcagcttgc tacaccattc actttgaatg cagatgaaat ggacaaattg atccaaaaga
    25081 ttgaagacgc aggtatcagt gtggtggatg aaaatggtga tccatcaatt cacagcttga
    25141 agagtgctga gaaaaaagct gaacaagcaa aaacagaaga tctatctgcg ccaaccggtg
    25201 taaaaatcaa tgacccggtt cgtatgtatc tgaaagaaat tggccgtgtc tcccttttaa
    25261 ctgcggaaga agaagtagcg ttagcattga aaatcgaaga aggcgaccaa gaagcgaaac
    25321 agcgtctggc agaagcaaac cttcgtttag tagtaagtat cgctaaacgt tatgtaggac
    25381 gaggcatgca gttccttgac ttgatccaag aaggaaacat gggactgatg aaggcagttg
    25441 aaaaatttga ctaccgcaaa ggatttaagt tttcaaccta tgctacatgg tggattcgtc
    25501 aagccatcac tcgagcaatc gctgaccaag cacgtacgat ccgtattcca gttcatatgg
    25561 ttgaaacaat taataaattg atacgtatcc aacgtcaatt gctgcaagac cttggaagag
    25621 aacctacgcc agaagaaatc ggtgcggaaa tggatcttcc aacagaaaaa gtccgagaaa
    25681 tcttgaaaat cgcgcaagaa cctgtttcac ttgaaacgcc aatcggtgaa gaagatgact
    25741 ctcacttagg tgatttcata gaagatcagg aagcaacaag tccagcagaa cacgcagctt
    25801 atgagttact aaaagaacaa ttggaagatg tactagatac attgactgac cgggaggaaa
    25861 atgtccttcg cttacgtttt ggtttagatg atggccggac gagaacattg gaagaagtag
    25921 ggaaagtatt tggtgttact cgggaacgta ttcgtcagat cgaggctaaa gcattaagaa
    25981 aattgcgcca tccttctcgt tcaaaacaat taaaagattt cttagaataa gcatgaacta
    26041 ttgaccagaa gagaaatcag taaaactgtt tggtttattt taaaagaaaa aaagcttgag
    26101 actacgcagt ctcaagcttt tttttctata ggaaggaaat tcattcatta tttttaagta
    26161 aatcctaaaa ataagctatt ggcgtgacgt atagaatatg atagattaaa tggtagcgta
    26221 aacctagtaa aggagccgat cgctaagtga tgaaaaaatg tccaaattgc ggatcgaaaa
    26281 aaatggagca gaatactatc tgtccagact gcggtcatca aataactgca gaagatcagc
    26341 aaataaaaga atcaattgaa gaaacaaata cagatgcagc tatgcaggat aaatcaactg
    26401 aagatgcagt ctttcctgat acagaattaa acgatccaat cgaatggtcg gaattgaaag
    26461 atttacctct agaatctgta atggaattat tcgaaaaagc agaaccagaa gacgaagaac
    26521 cacctgctca cgatcaagta actaatatag agaaaaatga acagaagaaa gcaactgttc
    26581 atgagacttc ggaagaatca ggaaagaatc aagaagaaac gcttgaatca aaacaaaaaa
    26641 gaagagtagc tgaactgaaa gaaatcgtcg ataatgagga agaaaattcg atactatccg
    26701 cttatatcaa agcacatcgt gaagatacaa aagaagagca cgcgaaagag ctgctcaaaa
    26761 tgatcagtga aaaaattgca caggaaaaca aagtagagcc agaacatgaa gctgaaaaag
    26821 acactgaaaa agacattagt ggagtaaacg ctgcaggtaa agaatcatta aaaacgaatg
    26881 atctaaaaac ggatatcccg gaaaaaactg aaacattgtc tgaaaaacaa tcagaagacg
    26941 agaaatcgaa tgggttgccg gaagcagata aaaaagaaat gctcgaaaaa gcagacaaga
    27001 aggcaacaga gtcggatgga ataaataatg aagccgatgc tgaaactcaa tctgcaagtg
    27061 aagatagcac tgctcttgaa gctaaaatag aaaaagaaga gaaccagcct gagctgattg
    27121 aagcagaaac agaaccaata acaatcgtgc caaatactga aaaaacgcct caagaatcaa
    27181 acccagaaaa aaaagattta gataagacac aaaagaaaaa atcaaaaaaa gtgccgtatc
    27241 tcgctttagc agcagttctt ctactaggtg ggggaggctg ggcttattat gaccatcatc
    27301 aaaaagtaga agccgaagta gcagcagaag caaaaagaca gcaacaaatg gaagaacttc
    27361 atgagaaact tgctgccttt tatacagatg atcaccatca atttattcgt gcagatatga
    27421 ttcatcaaga tttatcaaaa ctaaaaaatg atttatccag tcttaagaat gaaaaaggat
    27481 attcagagtt agaaacaact taccaagata tccaatctaa gatccgagat atccaaaaag
    27541 tgaatgagtg gttcatagca cctgccatcg tcgatgatca tctagcagat gacatcaagt
    27601 tgaaagcaga taaagagatc caagcaatcg aaacggataa cacagatttt ggaaaattga
    27661 ttcaacaagc acaaacagaa gctaaaaatc aatatgctga attgcaaaca gctaaagaaa
    27721 aaacagctat agtctttaaa gacggaaaag tagtggattc agcaacacgc gaccaataca
    27781 agacggcaaa agaagcagta gaaaaagtga aaaatacaga tctatctgct cagttgaatg
    27841 aacaattgca acaagtagat aagatgctca ctgataaaga gaaaaaagaa gaagaggaaa
    27901 agaaaaaagc agaagaagcg aaaaaagcag aaaaagcgaa aaaagcagct gaagaacaag
    27961 caaaacaagc cgctcaagca gcagctgcag ctcaagccgc tcaacaagct gcggtcgcac
    28021 aagcagcaca gcaagaagcg gcatcctcga ccccttcctc ttcaaactca gcgaatcagc
    28081 caatcaaatc tgccagagca agtgatattg ccgatgcatc taatccagca tggacgtggg
    28141 caccaggtgt ccaaagcaaa gtgattgcta catgtatcca acgtggatat atcgttgaag
    28201 gaggatacag attagaaaaa gccagaatcg aaaatgggga aggctattac aatctatatg
    28261 ccacttcaac aaaatctgca ttgatgaaag gcattggaga aagtgctttg ccattttaca
    28321 tcgtaaccat caactgtaaa actggatggt tcggaggaaa tgggagcaat taattttagg
    28381 caaatgaaag aggctgtgag cctgccgttt aacaccgttt attcgaaaaa ggggagtatg
    28441 acacaactgt tgtcatactc cttttttcac ctttccaaac caaggctcag ctattcaagt
    28501 agataacatt atgttatgat agcaaagaaa tgtattaaga aaacctttca ctatagaggg
    28561 agaaaaaaat gaacgattta ttagcaacta tatttacagg tattattatt gatgaaaatg
    28621 aaaaaaacta ctttgtccaa aaaaacggac aaacctttgg cttaagcaaa tcagaaggtg
    28681 aacacaaaat cggtgaagca gtggaaggat tcggctacca aaatcaaaaa catgaaaatc
    28741 ggatcactac acaaattcct aaaagccgta tcggtcatta cgcttttgga atcgttactg
    28801 ctacacgaaa agatttaggt gcgtttgtcg atatcggtct tccagataaa gactttgtcg
    28861 tatctcttga tgaattgccg acaatgacag agctatggcc taaaaaaggg gatcgcttga
    28921 tgatttccct tagagttgat gcaaaagacc gtatttgggg cagtttagca gatgaaaaaa
    28981 tattcaaatc tttaagcaaa tatggaacag aagaaatgaa aaacaaagat gtgataggaa
    29041 cagcataccg cttgaaatta acaggtacgt acgttttaac ggaagatttc tatcttgggt
    29101 ttatccatcc ttctgaacga tataatgaac ctcgtctagg cgagcaaata aaaggtcgag
    29161 tgattggtgt ccgaccagac ggtgtactga atctttcatt gaagccaaga gcatatgaag
    29221 ctatatcaga tgatgcacaa atgattttaa ccattttaga acgttctgca gatcagcaaa
    29281 tcaactttac agataaatcg gatccagaag aaatcatgaa agcttttggt atcagcaaag
    29341 gagcatttaa gcgcgcatta ggtaatcttt tgaaacaagg attgattaca caagaaaacg
    29401 gtgtgacaaa actagtcaaa aaatctaatt gagaatccct tgcatgtttt tcttaactag
    29461 cttataataa ttttaattaa catagtgtta tatctttttt tatttgtaat tattataaat
    29521 tgttggggtt gagtaaatgg tcatgaatgc tatttcggcg atgaaaaaaa caaaacagca
    29581 gctacatgaa gcggggttta agctgacacc gcaaagagaa gctactctgc ttgtgctttt
    29641 ggaaaatgag aaagagcatt tatctgcaga agaaatcttc tttttagtga aaaaaaagag
    29701 tccggaaatt ggacttgcta ctgtttatcg aacccttgaa atcttgacag aattgaaaat
    29761 tgtcgataaa gtaagtttta atgatggatt agcccgttat gatttgagaa aagaaggcgc
    29821 caaacatttc catcatcatt tactatgctt gaactgcggc acgatcgagg aagtagaaga
    29881 ggatctttta cttgaagtgg agcaagtagt agaagaacgt tatcattttt tagtaaaaga
    29941 tcatcggcta acgttccatg gaatctgtca ggaatgctat gaaaaagaaa aaagaaacca
    30001 gtccgtctga gtgtctagac ggctttttct ttgctcagat tatggaaaaa gactatcaga
    30061 atgaagcggt atctgatatg atagagaggc atgaggggga ttattatgaa agagcaaatc
    30121 aacgattatc tccactactt gaatattgaa cggggattgt ccgccaatac acgacaaagc
    30181 tatgaacgtg atttggaaca gtacttgcat tttctacagg agcagaaaat tgcttcatgg
    30241 caggatgtcg atcgcttttt agtcatccgt tttctggaga agttgcatga agaaaaaaaa
    30301 gcgacagcaa ctgttactcg gatgatctca agtctcagaa gattccatca gtttttacgg
    30361 caagagcgta tcactgatca tgatccgatg caacatatcg atacgccgaa gaaagcacag
    30421 aaattaccaa gtacattgac tctgacagaa gtagaaaagc tgattgaagc acctgatacg
    30481 acaaaaacgt tagggataag agatcgtgca atcttggaag tcatgtatgc tacagggatg
    30541 cgcgtcagtg aattgattgg attgaaattg ggagatcttc atctttctct tggactggta
    30601 cagacagttg gaaaaggaga taaagagcgg atcattcctt taggtgatta tgcgatccaa
    30661 tggctggaac gctacttaga cgaagcacgt ccattacttg taaaggacgc atctgaaatg
    30721 catgtatttg tcaataatca tgggaaaggt ctttctcggc aaggaatctg gaaaaatctg
    30781 aagcagctag tacgagaggc tgggatttac aaagaagtaa caccacatac gctgcggcat
    30841 agctttgcca cacatctgct agaaaatgga gctgatttgc ggacagtaca ggaattgtta
    30901 ggtcacgcgg atatctctac gactcagatt tatacacata tcacgaagaa aagaatgacg
    30961 gatgtttata agcagcattt tccaagggct tagtcatagg aaggtgagaa gatgttaaca
    31021 cattaccgga aagaaaaccg taaaattgct atgggactat tgagtttcca taagaaactg
    31081 acaggtaaaa cttctttgtt gaaggagatt gatacgtacg aatctgaaga cgattatgaa
    31141 ctttttttct ttacgccaga aggttcaacc aacattcaag ggattattgg tgtttactgg
    31201 gcaacgaacg atcagctgat catccatgac atttctttga atccgtctta tcgcggtgag
    31261 aatatgggct ttcaaatgtt agatgaatta caggaaaagt acccggatgt ctccatccgt
    31321 gcaactgaaa tcacagagcc ttacattgga aaatggctag caaagaaaaa gaagaaagag
    31381 tgaatgagtt gacggagatc aatattaaat tagatgtatt cgaaggtcct ctcgatctgc
    31441 tgcttcatct gattcagcaa atggaaatcg atatatatga tataccgatc gctacgatca
    31501 cggagcaata tatgaattat atccatacaa tgaaaacact agagctagct gttgcaggag
    31561 aatacttagt gatggctgct acattgatgg caatcaaaag caagacattg cttcctgtcc
    31621 aagaattcgt cacagatgat gaagaattat gggaagaaga tcctcgagaa gaattggtca
    31681 ctcagctttt agaataccgt aaatataaat atgcagctga aagactgaca gaaaaagcag
    31741 aggaacgaag tctttattac acaaaagaac ctatgaacat cgatgggtta gcggatgctg
    31801 aaaagccgtt gaagcgagga caagtcaaaa aaatcgattt gatccgtgcc atgcagaaaa
    31861 tcttagaacg taagaaattt tctcaaaaag tggaaaaaac gatcacgccc gatgacacaa
    31921 cgatcgaaga acgaatggta tttatcgaag agcgtataaa aagaaatgcg catggctgta
    31981 ctttcgattc tttttttgag tcaccttcta aaagtgaagt tgtcactact tttatggctt
    32041 tgctagaatt gatgaaaaaa gggcagatca ttgcgcaaca aaaagaaaac tatgaatcaa
    32101 ttatgcttta tccagcagta ggagataccg aacatgacga aattaagtga acttgaagcc
    32161 cttcttttta ttgttggcga ggaagggatc agtaatgaag aattaagcgg actgttaaat
    32221 ctcagcgaag cagaagtcac aagtttagtg aaagaattaa aagaatctta cactcaaaac
    32281 tcgcactcag ctttgcatat ttttgaaacg gaaagccatt atgtattaac gactaaaaaa
    32341 gagctggctc ccttacttaa gcgatatgcg caagggacat caaacacatt atctcaagcc
    32401 gctgtcgaaa ccttagcaat catcgcttat aaacagccaa tcacaagagc tgaaatcgag
    32461 cagattcgag gagttcaggt ttcaggagcg gtacagcgtc tagctgccca taaactgatt
    32521 gaagaaaaag gtagagtaga agggccaggt cggccgatat tatatggaac aactggctac
    32581 ttcttagatt attttggttt gaaatcatta gaagaactgc ctgatacaca agaaatggaa
    32641 atttcatttg aagaagacgt gtcaccagat ctattctttg atggaatgaa taaaggagaa
    32701 taattgtaat ggaaagattg cagaaagtaa ttgcccacgc tggcatcact tctcgtcgaa
    32761 aagcagaaga attcatcctt gcaggacgtg taaaagtaaa cggacaagtt gtaagggaat
    32821 tagggataaa agttgggaaa catgacactg tggaagtgga tgaagtccca atttatcaag
    32881 aagaatacgg ttattattta ttgtacaaac ctaaaggagt tatttctgct gttgcagatg
    32941 acaaaggaag aaaagttgtt acggatcttt tgccgatggt aaaagaacgt atctatcctg
    33001 taggtcgact ggattacgat acttcgggga tcttgctact gacaaatgat ggagattttg
    33061 ctcagcgcct gacacatcca aaacatgaag tggataaagt ttacgttgca aaggtaaaag
    33121 ggatcgctac aaaatcgatg cttgcacctt tgacaaaagg aataaagatc gaaggacgaa
    33181 aaacatctcc agctaaattc caaattttat cagtggatcc taagacaaac cacagtatcg
    33241 tggaattgac gatccacgaa ggacgtaatc atcaagtgaa aaatatgctt caagcagttg
    33301 gtcttcctgt gcaaaaacta aaacgtgaaa aatacggaga tctgacttta caaggcctac
    33361 gtcctggaga ttaccgtgaa ctgaacaaaa aagaagtcag cagtttgctg aaccactcaa
    33421 aatgaacact tactttttat aagtgttaga tgttgcgttt tttgaataga tatgtataat
    33481 gaatatgtaa acaaaaatta tataaggttc gtcttcaggg gcagggtgaa attcccgacc
    33541 ggtggttata gtccacgacc tattttgcaa aaaatagctg aatcggtgaa attccgatac
    33601 cgacagtata gtctggataa agaagataga gcttatttga cgacgcttga tcaaataact
    33661 ctaactctat tttccctgga aaatagagtt ttttcatttg gagagaaacg tcaaaaagtt
    33721 cgctgaagag gagtcctaac aggaggattt caaatgaaga acacccgtgt acagaaaatg
    33781 gttgcagtag cgatgtttgc tgcgatcgcg ttagttttac agtattttgc ttttccgatc
    33841 atgccggctt ttagtttttt gaagatcgat tttagtgata tcccggtatt gatcagcatg
    33901 ttcttattcg gtccagcggc cggagttgca acagcgttct tgcgttcagt attacatctg
    33961 ctgactacag gtttttctcc ggataatttg gtaggagaca ctgctagttt cttatcaaca
    34021 acgatcttta cattcccgat ttattatttc tttagtgtaa agaaacagca taaaggaaga
    34081 aacaaatttt taggtgtcat tacaggaacc atcgctctaa ctatttttat gagtatcgca
    34141 aattatttcg ttattacgcc tctttactta atgtttttag gactgaacgc aacacagatg
    34201 ctaggcatgt ctttagcaaa ctatgtactg attgggatcg ttcctttcaa cttgatcaaa
    34261 ggattcatcg tgagcgctgt ctttttagtg ctgcatgcgc aacttcttcc atggttaagt
    34321 cgaaaacaac atcagttcga acaacgtcat acagcagtca aataattatc aaaaagatca
    34381 agaaatttct tggtcttttt ttttggaaaa aaatacaaga catgcaataa tgtaaacgtt
    34441 ttatttgttt gttttgagta tttttttgat ttatgcataa aaatcaatca attctttgca
    34501 aatgtgaaat tcttctcctt ttaaaagacg aaaatgtttg tttataaaaa aaaggaaacg
    34561 ctacaatgaa aaagaagaaa gctttaaaaa cctgaaaaaa gaggagggaa tagggaaaaa
    34621 cgaacttttt gaacaaaagt tttacaaaat gcgtgttatt atttttgttc tgatactctt
    34681 tttgataaaa taacattaga gccaaaattg ttgtctctaa cggaaaagaa aggaagaaga
    34741 agatggaaca aacaaaaaag aaaaaatttc aaatgccttc agcgtatacg gtcttattta
    34801 ttattattgc agtaatcgct gtacttactt ggtttatacc agctgggcac tatagtgtag
    34861 acgatgcagg caacatcgtc gctggatcat atgaacgagt acaacaaaat cctcaagggc
    34921 tgtgggatat tttcatggcg ccaatcaatg ggatgttagg tgtaccagcg ggggagctaa
    34981 ctaaagaaac acctgctgca atcccaatct ctttcttcat tttggttgtt ggtggatttt
    35041 taggtattat caacaaaaca ggggcactag atgccgggat tgcttcagtt gtgaagaaat
    35101 tcaaaggaaa agaaaaaatg cttattccag tattgatgct gttatttgca ttaggcggct
    35161 ctacttacgg aatggcagaa gaaacgattc cattctatgc tttactgatc ccagttatga
    35221 tggctgtagg ctttgataca gtagtagcag tagcaatcgt attagtaggg tctcaagtag
    35281 gctgtctagc ttcaacggta aatccttttg caacaggcgt agcttcacaa gctgttggga
    35341 tttcgatggg tgaaggtatt ggtcttcgat tcttgatgtt cgttattttg ttagctattt
    35401 ctattgtata tgtgtatcgt tatgcatcaa agatcgaaaa agatccaaca aaatcattag
    35461 tatacaacca acgtaaagaa gatatgaagc atttcgatat tgaagcaatc ggtcgtcaag
    35521 aagtaatcac gaaaaaacaa aaacatgtta atgtattgtt ctttattact tttggtatca
    35581 tgatcatcag tttgattcct tggacaacct tgaatgcgaa tttcacgatt tttgaatcat
    35641 tgacaaactg gttaacaaac cttccggtac taggtactgt tctaggtaaa agcatgcttc
    35701 cattaggtga ctggtacttc ccagaaatta ctatgttatt cttagtaatg tccatcgtag
    35761 tagcaatcac ttatggcatg aaagagtctg aatttgtctc tgaattttta gctggtgctt
    35821 ctgatttgat cggtgtagca atcgtagtag ctgttgctcg tggtatccaa gtggttatga
    35881 acaatggatt aatcacagac accatcttac attggggcga acaaggatta agttcattat
    35941 cttcaagtgt attcatcatt attacattta ttttctatat ccctatgtca ttcttgattc
    36001 cttcaacttc cggcttagct gctgcaacaa tgggaatcat gggacctatg ggtgaatttg
    36061 ccggcgtcgg taaggattta gtaatcactg cttatcaatc tgcatctggt ttagtaaact
    36121 taattacacc aacatctgct atcgtaatgg gtgctgtagc aattgctcgt ttcgatgttt
    36181 cagtgtggtg gaaatttacc ggtaaattaa ttgcgatatt atttgtagcc atctgtgtga
    36241 tcctaggtgt agcagcaatg ttctaaacct agacaaatac aggtacaagg tctatcataa
    36301 gaatgaagaa accgataaac ggctatcgat aagcacgtgg acaagtcaat agctccattc
    36361 agtgagtgtt ttttctttca tcatttgtta agaacattct gcttgctgat ggagcaggct
    36421 taaaacgtca ttatttgaga agaagaggtt gtgtcggagg aatttgacat gacccttttt
    36481 aacatattag gaggataata tcatgaaaaa atttgtgaca gaacaacatc atgaacaagc
    36541 attagaatca ttgagcgaat tgatccgaat tccttccgta ctagatgaag cagattcagg
    36601 acaaggacat cccttcggta aaaaagtgat tgaagctttg gataaagtat tggaaatcag
    36661 tgaaaagcta ggatttagaa catttaaaga tccagaaggc tattacggtt attcagagat
    36721 tggctctgga gatgaattat tcggtatctt gtgtcatatg gacgttgttc cagcagggga
    36781 cgaaaataat tgggaaacaa aaccattcga tccaactata aaagacggct ggttagttgg
    36841 tcgcggatcc caagatgaca aaggtccttc tattgcggca atgtatgccg tcaaagcttt
    36901 gattgatgct ggcgtggaat tcaagacacg tattcgtttt atctttggta cagatgaaga
    36961 aaatttatgg cgttgtttag aaaaatataa cgaaaaagaa gaaggaatta cgcaaggatt
    37021 tgcgccagat gctgaatttc ctttaattta tgcagaaaaa ggcttattgc aagcttactt
    37081 gacaggacca ggaacgaacg agttttctgt aaaagctgga ggagcgttga atgttgttcc
    37141 tgatgcagct ccttactctg gagaaaagct ttctgaagta aaagaagcgt tgaaaaaaca
    37201 cgaattcgat ttcgaagaac aaggtgaagg catcgtcgtt cttggtaaaa gtatccacgc
    37261 gaaagatgca gcacaaggag tgaatgctat ttctcgtctg gcaattgctc tttcagaagt
    37321 attcgatttt ggcccaatca atttcttagg aaaattagtt caagaaaatg caactggtga
    37381 agctgtcgtg ggcaaaacag aagatgaaca atccggcgag ctaacgatga actttgcaag
    37441 tttggaaatc acaccagaac aaacaaaaat cggtgtagat atgagaatcc ctgttacatt
    37501 caagaaagat gatttagtag ctaaattaac taaaacagca gaaaaatatg ggttgactta
    37561 tgaagaattt gacttccttg attcattata cgtgccacta gacagtgagt tagtgaaaaa
    37621 tctattgggg acatatcgcg atattactgg agacatgact gaaccgtttg tatcaggcgg
    37681 cgctactttt gctcgtacga tgaaccagtg tgtggcattc ggtgcgatgt tccctgatac
    37741 tcctgacttt atgcaccagg cgaatgaacg atgggaatta agtagtatgt ataaagcaat
    37801 ggaaatttat gcagaagctg tgtatcgttt atgtgctaaa taaaaataaa catacaaaaa
    37861 gggcggatat tcaaatggat attccgtctt tttttatttg atgctggaaa gtttgcattt
    37921 ctctcttatt ttttttaagg tataatcata cttgattaag aaaaaggggt cactcagata
    37981 gaaaaggtgg gagaaagtga aaatatttag acgaatacta attggcttag tcatcatttt
    38041 aggattactc ggtggagccg gttattacta tatacagcaa aatacgtacg aaccatcgtc
    38101 agaagcgcaa gtgttggtaa aagaaggaaa aaacaaagaa gattacttat tttttgaagg
    38161 agagaaaaag aatcatggaa attctccatt acttattttt tatcctggtg ctctcgtaga
    38221 gccagaaagt tatagtattt gggcgcaaga tttagcaaaa aacggctttt cagtcgctat
    38281 tgtcaaaatg ccacttgatc tagcagtgat gggcggaaac aaagcggata aggtcaaaga
    38341 agattttcca aattccgatt atgtgatcgg tggccattca ttaggtggcg taatggcaag
    38401 tcgttatgtc aataagaatc attcagacaa acatttaaaa ggggtattct tcatggcaag
    38461 ttatcccgac aaaaaaggaa cattgacaga atcagctgtt cctgttttat cgatcacggc
    38521 gacaaaagat aaagtattga atcaggaagc ttatcaagaa gctaagcagt atttaccgga
    38581 aagaacagag tatgtatcaa tcaatggcgg aaatcatgga ggttttggca gctatggtga
    38641 gcaaaaagga gatggagagg cagaaatttc taacgcagac cagcaaactg tgatcgtcaa
    38701 tacattggaa aactggctga aaaagttgaa ataatataaa tgggtcctat agggtccgtg
    38761 aaaaaactag tcaattaact atagatagca aatgaatctt tcaaaataag tcgggatttc
    38821 acggaacaag agaaacagat cccggcttat tttcaagttt agagaactta gtgtacgttt
    38881 ttaaagtttc gtttatattt atattttatt ttctaataaa atcgctcgaa ctggacattt
    38941 ttgatacgct ttttttacat atgtttcttc ttccggcatc acgaactgtt gatgagcctc
    39001 tggttcttgg gcaaataaaa cgattccttc ttcatcataa tcgaaaatat ctggcgcttc
    39061 gatttgacaa agaccgcatg caatacagcg ttcaggtaca agtttacata gtagtgacat
    39121 ttctaaccat cctaacttaa ggagaataat atgattgttt ttattttatc gctattttca
    39181 tcaggacaca agctgcggat tagttctctt taccaactcc ttgtgggaaa acgaacgact
    39241 tcagtactta tttatggatt tacccacgaa cttttgttta tccataacag ttttccagaa
    39301 ttgaagcagg ataagtttta tcaaattttg caaaaaattg cgcagcaagg ttggatcgaa
    39361 ataaatgaaa atgaagcaaa gctaacgcct gcaggggctg atatgttgtc cgaacatcaa
    39421 atagaacata ctggtttgcg ttttgaccgc tatggcagaa caggggaaac tagttggcgg
    39481 ctcattaaat ttgctgttca agtaatttct aatctggcaa cgggtattca agactatctt
    39541 ccagcagaga caagtccttt ttatactttc caactaaaaa aatggcttag tgaaagccaa
    39601 ttgccacgtg aaattttgat tgatactgcg tatgagagtt tggtgcagct gttttcggag
    39661 ataccagaag aagcagcaga ttttttagct aaccaattat ctggaaatga gcgaacaggg
    39721 ctgcttcctt atcagctggc aaaaaacaat gatgagtcag cggtttatct gcaacaaagc
    39781 agatgtatcc accttttgtt agcacaaatc gaaaaacggc cagattcgct ttggcacgct
    39841 ttgatcgatt cattattaca gcaaaatttc aatcaaagca tgatgatcac caagcagatg
    39901 tttatgaatg gtcagacaat tgaccagatt atggcaatcc gtcatttaaa aagaggaaca
    39961 gtaacagatc atcttattga gtgggccttg ttttttgatg attttccata cgagcggatt
    40021 ctctctgcag aaacaatcga acgtttggaa ccaaataagg attccgtgcg agagtggcgc
    40081 tttagcgaat ggaatgtaga tggacaatta gattatggag aatttcgttt gtatcaaatc
    40141 tatctattga gaaaggaagc gatacagaat gttaataaat gaattaaaaa aatattttgg
    40201 atttgattca tttcgtcccg gccaagaaga aatcattcag gcgttgcttg atggagaaga
    40261 tactctcgct attttgccta caggaacggg aaaaagcctt tgctatcaat tgacggggtt
    40321 tttaatggaa ggtctggtca tcatcgtgtc accgctgctt tctctaatgg aagatcaagt
    40381 gacgcaatta caaaaacgag gggagaaacg agtagctgct ttcaatagtt tactttcgca
    40441 atctgaaaga agatacgtcc tccgtcatct atcgcaatat aaatttttgt ttttgagtcc
    40501 cgaaatgttg actaatcctt ctcttttaga gcatctgaaa aaacaaaata ttgcgttata
    40561 tgtagtagat gaggctcatt gcgtgtctca atggggagtt gatttccgtc cagaatatca
    40621 acagttagga aaaatccgaa agcttttagg aaacccggtc acactggcac taacggcgac
    40681 agcaacagat ttagtagcaa gagatatacg tcatgtacta tttgatcgac caccaaaaga
    40741 gatcagacaa tcagtcaatc ggcaaaatat ctcgcttttt gttcggaaaa cgcagcaaaa
    40801 agaacaagaa cttgaacact tcatggaaag agcacaagga gcggcaatca tttattgtgc
    40861 aacaaaaaaa gaagtcgaac gattgtatca tttattcagg gaacgtttta cagttggata
    40921 ctatcacggt ggtcttgatg cagctcaaag aagacaactt caacagcagt ttgtaaaaaa
    40981 tcagctgcag tttctcattg ctacgaatgc atttggtatg gggatcgata aatctgatat
    41041 ccgttatgtg atccattatg atttgccaga cagtctagaa aactatgtac aagaaatcgg
    41101 tcgagcaggc agagatcagg aagaaagtgc agcaattctt ttttatcaaa gtggggatga
    41161 acgtatccat tatttcttta atcaactttc tagagaacag cgacaaagtt ttgagcttta
    41221 tttggaatat gccacagagc aagctccctt tgatgagctg caaaaaaaat ggatggagct
    41281 gatccagcag tctgaaaaac cagaaaattg ggtggaacgg ctcaaacgtc aggaaaaaga
    41341 aaaagaattc cgcctgcagc agatgcttcg ctatattaat gaagaaaact gccgcagaaa
    41401 attcatctta gcttattttg gcgaaaaact ctccgaaaag ccgcagaatt gttgtgatat
    41461 tgatggagca aaagcagaac ttctgccaaa gaggaagctt ttttctaaga ctgaacaact
    41521 tcattgggaa acgattttac taaatttatt taaaaaaaac atggaacatt gattttttag
    41581 gacaagacac tttttcctat ggtataatac acacgataga gtttaggagg taaaacagtg
    41641 agcagaaaag atagacatca atctacagaa aataacgaaa cacaagaacc atgggaacaa
    41701 ccaatctacg atacagatga tgaagtttca tcaagaagtt ctcagagaca acaaaagaaa
    41761 ggaaatacat tatttctttc gctatttttg attttactgg tcttatgtat cgctattcca
    41821 gcaggtgctt atttctggat cagaaatgga tcaagcaaca atgcaacagc agcaacttct
    41881 tctgaaactt cgacatcagt agtagaaagt tctacaagta caacaaaaga ggagtcaact
    41941 gagacttcaa cagtagaatc tagtacaatc gcggaagaat ctactgtgtc tcaagataca
    42001 ggtgtaacgt cagaaagcag tattgcagca gagacaacac cgacttcctc aagtgaggca
    42061 acttatcaag caggggattc aagcagcaat gcagcagaaa caacaccttc ttcatcagct
    42121 gcagccggtt caacaacgac tgttcaagca ggcgaaggtc caaaacaagt ggctgagcgt
    42181 gcagggatca ccactgatca attgtttgaa ttgaatggct tagatccaaa caactttatg
    42241 ctttaccctg gtcaagaatt aagaattaaa taataatagc cgcgctttgc ggcttttttt
    42301 ctgagaaaaa ggagttatat catgacacaa atcagtattg caatcgacgg gcctgcttca
    42361 tctgggaaaa gtaccgttgc caagatatta gcaaaagaat tgaattatat ttatacagat
    42421 accggtgcca tgtatcgcgc agtaacttat ttagctatta cgaacaatgt atctttttca
    42481 gatgaagaag ggctagtttc gctgatcaaa aagtatccta tctctttttc gcaacaagaa
    42541 gatggacagc atgtattcat agcatcaaaa gatgtgactt tagaaatccg tcaaccagat
    42601 gtcacaaaag ctgtttctga agtctctgca catggaaaag tcagagaaga attagttgct
    42661 gtccaacgaa aaatagggga acgcggtggc gttgtcatgg acggaagaga catcgggaca
    42721 gctgtattac cgaatgcaga agtgaaaatc tttttagtag caagcgtaag tgagcgtgca
    42781 gaaagacgtt tcaaagagaa ccaagaaaaa ggaattccga ctgatcttga aacactgacg
    42841 aaagagatac aggaaagaga ccattatgat tcaacaaggg acgtctctcc gctgaagcaa
    42901 gcagaggatg ctgttcgtat cgatacgact ggcaaatcga ttccagaagt cgttgcagca
    42961 ataaaagctg ttgttttgaa aaaaggttat cttctttttt aaattaagaa aaaaaatgaa
    43021 aaacaattgt tttttcaagg aaaacgaacg aaatcgcttt attttttttg aataatcaat
    43081 taaactaaaa acagtaccgt acttacggca gaatgttggt taggaggaca agtgttatga
    43141 cagaatttga tcaaaatcaa gagaacaacc agtcaatgga agacgcaatg aaaagcgtac
    43201 aagaagtaaa agttggcgat gttgttaagg gcgaagtttt agtcattgaa gacaaacaag
    43261 tgattgtcgg aatcgaaggg gctggagttg aaggagtcgt tccggcaaaa gaattatcaa
    43321 ctttgccggt agaagatatc agcgaagtag taaaagtcgg tgatgtatta gatttagtcg
    43381 ttatcagtac gatcggcaaa gataaagaaa acggcagcta tctactttca aaacgccgtt
    43441 tagatgctaa aaaagtttgg gaagatatcg aaagagattt ccaagagggc aaggtcatcg
    43501 aagcacctgt tacaaatgtt gtcaaaggcg gtttagtcgt tgacgtcggt gtacgtggtt
    43561 ttgtccctgc atcaatggtg gaagatcatt ttgttgcaga cttttcagac tataaaggca
    43621 aaacattgag ctttaaaatc atcgaaatcg aaccatcaga aaatcgtttg atcttatcgc
    43681 ataaagcggt agtggaaaac gaaagagaag aaaagaaaaa agaaattctt tcaactatcc
    43741 atgagggaga tgttctcagt ggacgtgttg cacgtcttac tgattttggc gcatttgtcg
    43801 acctaggcgg aattgacggt ctagtccatg tctcagagat ttcacatagt catgtagcaa
    43861 aaccaagtga tgtattggct gtcaatgatg atgtaaatgt caaagtgttg tctatcaatc
    43921 ctgaacaaga acgtgtttcc ttgtcaatca aagacacaca gcctggacct tggtcagaaa
    43981 tcgatacaaa agctccagtt ggcacagtat tagacggaac agttaaacgt ttgacaagct
    44041 tcggtgcctt tgttgaagtg ttccctggag tagaagggct agtccatatc tctcaaattt
    44101 cacataaaca tattgcgaca ccacatgaag tgcttcatga aggtgacgaa gtgaaagtga
    44161 aagtattaga gatccatcca gaagaacatc gtattgcatt gagtatcaaa gctttagaag
    44221 aaaaaccagc ttcagaagaa gaaccaaaaa atgttgagtc ttatgaatta ccagaagaaa
    44281 atactggatt tactatggga gacattttag gtgatgcgtt gaaagaagaa gtagaagaaa
    44341 aagcaaccga tgattctgaa aaataattga ttttgtatct gtgactgaga tgaaagcagc
    44401 tgcttttgtc tcagtttttc ttttgaaaag cgattagtat ttcaataagg aataaaaata
    44461 cttcgtttaa ttgacaatct atgcttgaaa gttggcaatg cattcgttaa actaagtgag
    44521 attggaaact tgtgaggagg aaataatatg gcaaatccaa caattgcaat cgttggccgc
    44581 ccgaacgtcg gtaagtctac gatttttaac cgcatcgctg gtgagcgtat ctcgattgtc
    44641 gaagatactc ctggagtaac tcgtgatcgt atttatgcaa aaggtgaatg gttaggtcga
    44701 gaattcagcg tgatcgacac aggcggaatt gatcttggcg atgaaccttt tatggatcag
    44761 atcaagcatc aagcagaaat cgcgattgaa gaagcagatg taatcatttg tgtagtcagc
    44821 ggacgcgaag gcgtgacaga cgcagatgag atggttgcga aaattttata cagaagtaat
    44881 aaaccagtga ttttagcagt caataaagta gataatccag aaatgagaaa cgatatctat
    44941 gaattttatt ccttagggct aggcgatcct tatcctgttt caggaagcca tggactaggg
    45001 attggtgata ttttagatga agcagtgaag tatttctctg aagagactga agaagaggac
    45061 gacgacacga ttaagtttag tttgattggt cgcccaaatg ttggtaaatc ctctttgatc
    45121 aatgcgattt taggagaaga acgtgtaatt gtttcggata ttgaaggaac gacccgagac
    45181 gcaatcgaca cccacttcgt ttctgaaagt ggtcagaaat tcttaatgat agatacagct
    45241 ggtatgcgta aacgcggaaa agtatacgaa aatactgaaa aatacagcgt gatgcgtgca
    45301 atgcgtgcca ttgaccgttc tgatatcgtt ttgatggtac tgaatgctga agaagggatt
    45361 cgggaacaag acaagcgagt agcgggttac gcccatgaag ctggtcgtgg aattattatc
    45421 gtcgtgaata aatgggatct tgtaaagaaa gaaaccaata caatgcgtga tttcgaacaa
    45481 gagattcgtg atgaattccg gtatcttgat tacgctccaa tcgtttttgt ttctgcagtg
    45541 actaaacaac ggttggaacg tctgcctgaa atgatcgaac aagtaagtat gaaccagaat
    45601 cttcgtatct cttcggctgt gttgaatgat atcattatgg atgctgtagc aatcaatcca
    45661 acaccaacag ataaaggaaa acgcctgaag attttttatg ctacacaagt cgctgtcaaa
    45721 ccacctacat ttgtcgtttt tgtcaatgaa gaggaattga tgcatttttc ttatgaacgt
    45781 ttcttagaaa atcaaatcag aaaagcattt acttttgaag ggacgtccat ccgaattatc
    45841 cctcgtagga gaaaataggc attttaccat atcgacgttt ttttttcaaa agaaaaagac
    45901 agtttcgttt gttacgtttc tatgactaaa aaaagcatgt gaaaattggt aaaaaccttg
    45961 ctattaagcg gttcatatga tatcgtgata acagaatatt gaagttaatc aatatttctt
    46021 tttaaacttc tggaccactc agaaagtttt aactaaatga tatttctatc atatctcttg
    46081 tggaggaggt gaatctatcc atggcaaaca aagcagaatt aatcgaaaaa gttgcttcag
    46141 ctactgattt aactaaaaaa gatgcaactg cagcagtaga tgctgtattt tcaactattc
    46201 aagatgcttt agctaacggc gaaaaagttc aattaatcgg ttttggtaac tttgaagttc
    46261 gcgaacgcgc agctcgcaaa ggtcgtaacc cacaaactgg tgaagaaatc gaaattccag
    46321 ctagcaaagt acctgcattc aaaccaggta aagcattaaa agacgctgtt aaataagaca
    46381 gttgaaaaag cgcttgtggc tctagggcta caagtgcttt tctttttatt tcctagcaga
    46441 aaaggaataa ataagagaca gagattccga atccgtatgt catacataga aaacacatgt
    46501 attttattta atttttattt atttgcaagt tatgaggtga gctttgctct tatcttttct
    46561 agaatttgct tactaatggt aaaataaaaa taagaagagg agggatgcgt atggaacttg
    46621 attatcaagt agaagatgcg ttgacgaaag tgttttacgg agaaacgcta gtcagtcagc
    46681 taagagaact ttccattgat cagccaatct tatttttaac gaatcagcgc tattatgatt
    46741 tgtttgcaga taagatcaac ccgttatttg caaatcaagc aaacaatgac tggtatattt
    46801 gtgccaacac tcaatgcaat catttaacag aattaaagaa cctgcttgat ttcgtcaaac
    46861 gctatcccga aaatcaatcc atgtttatca tcggatttgg aaatgaaggg atcatggatc
    46921 tggcaggttt ttttcaaaaa catacaaccc ttgattcatc actatggatg atccctgtat
    46981 cgatccgttc tctaagccga gcattaacac cgcagcggac gattttgcag ctaccaaatc
    47041 gtgtagtgct gcaaaccatc aatctgccag aacgcatcgt ttatgatcaa acaatcagcg
    47101 agcggcaggt ggacggaaag cagattgact ttttgacttt tatctgttgc ggaatgattt
    47161 gtgatcatcg ttttttgcaa aatctgtata aaaactatcc aaataaaaag caattaacga
    47221 ctcggccgtt tgccgggatg atcgaagaaa tgatctattt ttatcaatta gaggcagaaa
    47281 acctgtcaaa ttatggtaaa ctgtttgaac aagcttttta tctgaccgaa aatggccatc
    47341 tactttctgc tagtatgaaa aaatttttag gaatgatgtt tcatttcgtg tggaatctcc
    47401 aagcatatcc ttttgatttc caactaaaaa acttttttgt gtggctggac ttgcttggct
    47461 atccaatcca ctggctggaa cagatttcaa agatggagta tttggaaaaa gttctttttt
    47521 tggcagaaaa aagcaaaaaa atacttgttt tagaaaaaat aggtataata gacggatacc
    47581 aacatccaaa cgaaaaagaa ttattgagaa caatgacggt atttcaacaa atcgtcagtg
    47641 agataagagg gaattgaaat gaatacaaat agcgaaaaaa tgttgcaagc tttatcagat
    47701 gaagacttgt cacaagcaca aatcttttta gaaaaagcat taaaagaaga cccagcagat
    47761 atattagctg aactaggcga agaattgctg gctattggct ttttgatcga agcaaaacag
    47821 atttttgaac aactgatcaa ccaataccca gaaaatgacg gactgaacat cccgttagca
    47881 gaaattgcaa tcgaagataa tgagatcgac ttagcttttg aatacctgga aaaagtggat
    47941 aagcaaagtg attactatcc gcaaagctta ttggcgatag ctgatttgta ccaagttatc
    48001 ggaatccctg aagtgagtga agcaaaattg aaagaagcag ctgggttgct tcctgatgaa
    48061 ccattgatcc agtttgcgct agctgaattg tatttttcag ttgatcgttt caaagaggca
    48121 gcagtggttt atgaaacatt gttggcaagc gggataaatg aaatttcagg aatctcgatg
    48181 caggaacgtt taggacaatc cttaagtatg caaggagaat tcgaagcagc tatcgtacct
    48241 ttagaagaag cactgaaaga agaaaggaca gatgatcgac tgttccagct agcttttacg
    48301 aatcttcaat tgaaagaaaa tcagcaggca atcaactatc tggaagaact tcgagaagtt
    48361 aatccgcatt atcaatcgtt atatctatat ctaggacaag ctcttcaaga agaagaaatg
    48421 attgaagaag cacaaacagt gttagaagaa ggaatcaaag aaaatcctta ccaagtcgaa
    48481 ctttatcacc tggcctcgga aaatgctttc cgtctgcatg atagacagcg agcagaagaa
    48541 cttttactga aagctttaga agtaggagac aaacaagatg aaacattact tactttaagt
    48601 aatctttact tggatgatga aagatatgaa gatgtgatac aagtgatcaa tcagatggaa
    48661 gaaactgcta atccttatgc agaatggaac ctagcccatg cttataatga attagaagac
    48721 tttaccgttg cagctgtaca ctatgaacag gcttatcatg aactatcaca tgaaccagat
    48781 ttcttgaaag agtatgcctt tttcttaaga gaagaagggc agctgaaaag agcgcaagaa
    48841 ttattgactt attatttaac acttgaacca ggagatatgg aggcactgtc attattagat
    48901 gacctaacag agaggtgaga aaaatgctaa ttgatgtacg ggaaaagaag aattttttaa
    48961 cgtggatggt caatcatgta tcttttagcc gaagagaagt tttttggatc ttgaattatc
    49021 ttgccaatca tgaagcgatt ttaagcaatg tccactttgt tgaaggggcg aattttgcgg
    49081 atcgaggact gcagatcagt gatctatctg ttgaagggga acccatgaaa ttgtttttgc
    49141 agggaaaaga atttacggac acagaccaga tttttcatga aatccgtttg aattggaaaa
    49201 agcctttata tgtggaatgt atattcgaaa atgcttggca gacaagagaa tatctatcta
    49261 tcttagaaga caatccattt tcttcttggg attcttcagt cagtgaagaa gatactgaat
    49321 taattgaacg atattttcaa gaaaaagaag aagaggcaaa actaaaaatg ctttatgctc
    49381 aaatcgatca agcactagaa gatggagata gggatgcttt tttgaaatta tctgatgaag
    49441 ttaaccgaaa gatccttttg cagacacaaa agaaaagtaa ccgggtaagt gaacataaca
    49501 attagttaaa aatagtatag tactaaaaaa gacaaaggtc agctggatgt ggaatctttc
    49561 ctcatagcct atctttgtct tttttcttat agttttttca gtaatcttgt ctgtggcttc
    49621 aaataagtaa agccttgccc ttccacttca tgcacattga tgattgaaag gaaagctttt
    49681 tcgtcaatct cgtggacgat cctcttagct tcagttaatt cactagagct aacgaccata
    49741 tagatgattt tttttgaatt accagagaat gcgccttctc cagaaaggaa agtcacacct
    49801 ctttgcagac cggtcatcag tagtggtgca atctcttcgc ttttgttgga gacgacaagt
    49861 agaccttttg cagaatatcc gccatctaag atcgaatcaa tcacacgact gaaaacatac
    49921 gaaacaatca acgtatacat catacgtttc aagtcgatat aggttaatga agcaagtaag
    49981 acgatgatat caaatgccaa tagggaacgc cccatagtga tgccgtaatt ttgttctaaa
    50041 atccgagcga taatatcact gccgcctgtt gttccaccca tgcggtagac gatcccgctt
    50101 cctacacctg caactaaacc cgcaagtaga gaaacgatca gtagatcatg ctgtaaattg
    50161 atttctagtg gaaccctttg ccagatccat aaaaagaatg acaaagaaag tgtacctaga
    50221 acagtatagg ccaaggaacg tttaccgagg atctttcctc ctagtaaaat caatgggatg
    50281 ttgattaata aagtcgtata agctggatca ataccaaata aagctcttaa gatcaaggtg
    50341 atccctgaga ctcccccttc tgctaaatga ttagcgatgt tcaagtaaac aagaccaaat
    50401 gcataaatac ttgttcctat aatgataaaa agaacttctt ttactgtttt tcgtttgacc
    50461 atatgaattc cagctttcaa ttatgtattt tcacagaatt tccatttaga aattccttcc
    50521 taaatgtagc atggaaattc gattttctca attgctctta tctaggattt gcgttaaagt
    50581 tttggtaaga tagttttgga gggaaaaaga tggataaaaa gcgttcttta tacagtatgc
    50641 agcaggaagt ggatgaatat atccagcaat tcaaggtggg gtatttctct ccattagctc
    50701 agatggctcg attaactgaa gaagtaggtg aattggcaag agaagtcaac cacacttatg
    50761 gagaaaaaag caaaaaggct tctgaaccag ttaactcggt agcagaagaa cttggtgatg
    50821 ttttattcgt tacgatgatc atggctaatt cattgaatat cgacttaact gaagtttttg
    50881 aaaaaaacat ggaaaaattc aatcaaagag accataatcg ctttgagcgt aaggacgagt
    50941 aaacaagatg aagtggtcct ggccttgaag actctgaaac ggacttgtca aacagtcacc
    51001 atgcaactga gtctttatct caggacaaga aacacgcagg aaaggttgga aaaatgaaat
    51061 taaaacaatt acctctggaa taccaaaagg caattccagt tctgaaaaaa ttagagaatg
    51121 caggatatga agcttatttt gtaggtggaa gtgttcgtga tatcttgctg aaccagccaa
    51181 tccatgatgt ggatatcgca acaagcgcat ttccagaaga aatcaaacag ctttttccaa
    51241 aaacgataga tattgggatc gaacatggaa ctgtgttggt attagaaaat gatgaacaat
    51301 atgagatcac cacatttcgg acggaatcaa cttatcagga ctaccgtaga ccagatcatg
    51361 tcgaattcgt tcgttcgttg gcagaagatt tgaaaagaag agattttacg atcaatgcgt
    51421 ttgctttgaa agaagatgga gaaatcatcg atttgttcga tggtctgcaa gatctagaag
    51481 aacagatctt gcgagctgta ggcaatcctc atgagcggtt ccatgaagat gccttacgaa
    51541 tgatgcgcgg gttgcgtttt gtcagtcaac tagggttcaa attagaggaa gaaaccttct
    51601 acgccatcaa agaaaaccac gagctattag gtaaaatatc tgtagaacgt ataaatgtgg
    51661 aattcatcaa gatgctcttg ggaaatgaac gggccgcggg aatccatgca tttattgaaa
    51721 cagagtgcta catttgctgt cccggtttga aagaagaagg cgaagcactt ttacgttttg
    51781 ctgaattacc atccgtccca ttgaaagaag agagccaagc atgggcatta ttgatcagcc
    51841 aactggatct aaaagaaaat gagattcgtt cctttttgaa atcgtggaag tgttctaacc
    51901 aaatgattaa agacgttcaa gcacttgtcc aaggactacg acaacgagaa actggagctt
    51961 tggaaccatt cgaattgtac ttcttaggca aaaaacatgc actgcaagta gaagaattga
    52021 tgatctattt tgacgaaaca cctcagatgg atgccgtagg agaatcctat gatcgtttac
    52081 ctatcaagtc attaagcgaa ttagcagtag atggtaaaat cttgctagaa gaaactggaa
    52141 aaaaacctgg taagtgggtt agtgaagctt tgcaattagc tgaaaaagcc gtcgtagaac
    52201 aaagaataaa aaatgataaa aaacaggttt tggatttttt atcgaaagaa aaattgattt
    52261 aaaaaacact agactgtatc tgctcagctt tgttggctac ttaaagtggg cagatacgtc
    52321 attagaagaa aaaacataag atgtgaccac ttacataaat ttgtgctaaa ctgaggtagt
    52381 acaagaataa attacatcat aaaggagtct ttttaatgag taaagaaatg acagcgctaa
    52441 aattttattt tagaaatgga gaaacctgga cgatcaaccg ccgtcatatc ggcgatcttt
    52501 ggatcaaaca aatcacaaca agttttggac gaatcaatgg cagtgaattt gttgaaatcc
    52561 atccgtgtgc tggatttaaa atcgaaatct tccatgaagg ggacgcagtt gcgacacatg
    52621 atatcaacct aggcggactg gaaatgggca tgtttaatcg cgcattgaaa tacgaagata
    52681 tcgagcgaat ggaaatcctt taccgaaatg gcacacctga tcttgtgtac ttcccatatc
    52741 tagataaagg aacagaagga ttagacaatc aatatcaatc aacaaaaatc agcgaaaaaa
    52801 caggtaactt gtatatcgtg atcaatccag atcaacgtgt agaagatgta tacggggaat
    52861 ttttcgaata aatcgatttt tacgcaaaaa tgaggctgtg aaagagatgt gtcttattac
    52921 tactgatagg ctgtctcttc cacaccgttt attcagaata agggactgtg acaagatttt
    52981 tgtcgcagcc ccattttttt gttgtgataa aagaacaaga aaggcaggaa tgggaaagta
    53041 aacatccttt tgtcagaaac ttttatcttt cttaagtttt acttgatatc tgatactatt
    53101 tggaatagaa tgaggcgttg aaaaatttat ggaaatttct aactagcaat aaacgatgaa
    53161 acaatgaaat atttttcatc caaaaagaac tttgctataa tgatggaagt ttgtgtatag
    53221 gagatctgga aaatgagaga gagttttgaa caacaaaaaa aactgttgca tgatcgctat
    53281 ggcgcccttt cgatggatga ccgccgtcaa atactttgta agttgcgtaa aagaaatata
    53341 ttgatgtatc gtcagttgga acgtctgaaa catgatctgc ttcgtttgga gtcaaaacgt
    53401 gtacagtgtg aattagaggg gaaccaaaca caggttgaag tagtcgaaac caaaatttta
    53461 aagaaaaaag aacaattttt aaaaatgctt acacaaaata aaaaataaag gggatggacg
    53521 atggaatttg ctgagttagt tattgtattt gcagtaacga tcaccttatc gaacatagct
    53581 agccgcattt tgccaatgat tccagctcca ctgatccaaa ttttccttgg agtggtatta
    53641 ggtctgacag agtggggaca atcaatcgat tttgaacctg aattgttctt agttatgatt
    53701 attgcaccat tgttatttag agaaggagaa aaagcagata tttcttcgat tctcaaaaat
    53761 ttcgggacaa ttttgttctt ggcatttgga ggagtcattt tgacccttgt cggggtaggt
    53821 gcgacgttat cattcctgtt gccaagtgtc ccacttgctg cctgctttgc ttttggagca
    53881 gcgttaggtc cgacagatgc ggtagcagtc agttctcttt ctggacgagt gaatatccct
    53941 aaaaaggcga tgcatatttt ggaaggagaa ggtcttttga atgatgcatc tggtgttact
    54001 gccttccagt ttgctcttgg tgcgctgatc accggatctt tttcagcagt aaacgcgggg
    54061 atgagtctag tagtttctag tattggggga gctttgatcg gctttttgct tgtctggttc
    54121 aaacaaaaaa taattcattt gatcgaaaaa gcatcagcac aagatgtcac agggtatctg
    54181 ctgatcgaac ttttgcttcc ttttctagcc tatgtattgg cagaggcatt tgatgtatct
    54241 ggaatcattg ctgcagtggc agcaggtatt ctccaagctt ctggttttag gaaaatctct
    54301 gtgtttgatg cagaattgtc gagcttgtcc cacagtacat ggacaactat tgcttttaca
    54361 ctgaatgcat tagtatttat atttttagga atcgaattga cgcaagtgtt ctcgccagta
    54421 tggggagacg gtttatatcc aaacggtctt ttattagcga ttatcgtatt aatctctgtc
    54481 atgctttttg tgatcagatt catttctatc agtctttttt atgtattcaa agatggcagt
    54541 aaaaagttca aaaaacagtt gaacgagatc ttgatcttaa catttggcgg tgtaaaggga
    54601 acagtcagtt tggctacgat ttttattttg ccattttcaa tcaataatat gatgttttac
    54661 cagcggtctt tgcttttgtt tctgacggcg ggtgtcatct tagtgacttt ggtcataggg
    54721 atcattgtct tgcctatgct gacggaaaca gaggaagcga aaagcacgga tttgaatgct
    54781 ttgatgattt tagaagaggt cgttgaagtt ttgcgaaaag aaataaagga aattgatcgt
    54841 aatacaaaag aatttttagc cactgaagca gtgatagaaa attatcaaga acggatacgt
    54901 gatctgtact tggaagattt aaccgatgat gaaaagcaag aagtacaaga aatacaagca
    54961 ctgattttgt cgatcgaacg ggatggattg gatgaaagtt atcgttcggg caagctttct
    55021 tctaatggtt atcgttttta ctcccgtttt ctttctcgat tcgaacattc tatcacaagc
    55081 cagattttgt cttttatcgg cttttggttc attgttgtcc gtaggttaat gcgaatcgtt
    55141 ctccatccga aaatgttttg gcaaagaaga tatgctgatc gtcagacatt tattagtgat
    55201 gaagatatcc aagaaatcag agagacatat caaaaaaata ctcagttgat tattgaaagt
    55261 ttagataact tgactgatat ttatgatgaa acgatggttc aattttttat ccagcaacgc
    55321 aaacttgaag gaataaaaat gaactcgggg aatttgattt cttcgtggat gatccagcaa
    55381 gatagtctgt ttacgaaaaa aatgctaaga ggttattatc ttgaaagaaa aatcattgat
    55441 gaatacgaag ttgctgaaaa aatcacgacg ttttctgcga acagctatcg caggaatatc
    55501 aatttgttag aatcttatac gatgaacaaa ccatctgata acttttcttt ccgatttgcg
    55561 tttcgagcaa agtcaaaaaa aacgagtcca ataaaataaa aaggtaaaaa ttttcaagtt
    55621 ttataaaaaa caaaacatat cagaacagga tcgaatcggt cctgttcttt ttttgagaac
    55681 acaaaaagaa caaataccaa aaaaattttc ctgaagttca ctatttttat gatacaatat
    55741 ccaagataag gagggaacat gttgaaagaa ttaaaagtaa ctgacctcaa aaaaacttat
    55801 ggtgaaaaag atttgtttga ccagatttct tttttggtcc acgaaaaaga ccgtatcggg
    55861 ttgattggga cgaacgggac gggtaaaaca agtctcttaa atattattgc cggtatcgat
    55921 agcagcgacg gtgatcggca aacagtgttt tatccaactg attaccgtat tggttatctt
    55981 tctcaagcag ctgaattttc cgatgaatta actgttctcc aagcagtctt tcaaggaaac
    56041 agccccttga tccaaactgt tcgagcatat gaggaagcat tgatcgaact aggggaaaac
    56101 ggagaagacc ctgatgtgca aaaacgttat gctaaagcag aagaacaaat gaataaagaa
    56161 gatgcttgga ctacagatac caacgcaaag attattttgc aaaaattagg aatcagtgaa
    56221 ttagataaaa aaatcagtac tttatcaggt ggtcagaaaa aacgagttag tctggcgcaa
    56281 gtgttgattg atgaacccga tttactatta ctagacgaac caacaaacca tttagattat
    56341 gaagcgatcg agtggttgga aaactatttg aaacaatacc gtggcgcact attgatggtt
    56401 acccacgatc gttattttct agatagagta gcaaaccgaa tcttcgaact ttcatttgga
    56461 aaattatacg agtataaagg aaattatgaa gcttacgtct tagaaaaagc agaacgtgat
    56521 cgtgtagaag tagaacaaga agaaaagcgc aagcggcttt ataaacagga attagcttgg
    56581 atgagggctg gcgtacaggc acgtggcacc aagcagcaag cacggatcaa ccgtttcgaa
    56641 gatctaaagg agaacttgta ccaagtaaac caagaagatg atttagagct gaacctagcg
    56701 acacaacgtt tagggaaaaa agtactggaa atcaaagatg gcagttaccg tataaatgac
    56761 caaacactgc tagaacattt agatttattg attcaatctc gggaaagact aggtattact
    56821 ggtaaaaatg gtgcgggaaa atcgacattg cttaatattc tagctggcag aattcccctg
    56881 gatagcggta caatgtctat cggtgaaacg gttcatcttg cgtattatac acaagaaaat
    56941 gaagaaatgg caccagataa acggatgatt gcttacttac aagaagctgc tgaagaagca
    57001 aaaacagcag atggcagtca aatcggagta gcagaattac ttgaacgatt tttatttcca
    57061 agatttatgc atgggacact gataggcaaa ctttcaggcg gagaaaaacg ccggttattt
    57121 ttgttgaaac tgctgattca gcaacctaat gttttattat tggatgaacc gaccaatgat
    57181 ttggacatag caacattgac gattttggaa gattatttta gaagttttcc aggtgctgtg
    57241 atcactgttt cccatgaccg ttatttcttg gacaaggtag cagataagtt attagtattc
    57301 caaggaaacg gaaaacaaga actttattac ggaaacatgt ccagttattt actgaagcaa
    57361 aaagagaccc agcagcctgc tgaaaaagcg aaaccaaaaa tacagagcaa ggaacctgct
    57421 gaaaagaaga aattatctta catggaacaa aaagaatggg aaacgattga agatgagatt
    57481 gcagaacttg aagaaaaaat ctcattgctc caagaagaga tgaatcatca aggagataat
    57541 ttcactcgat tacaagaatt gcaaaacgac gtatctgaaa cagaagcaca acttgaagaa
    57601 aaaatggcac ggtgggaata cttgagtgaa tgggtagaag attaggagga aaaaggatgg
    57661 aacaagcgta tcttgactta ggaagaaaat tgttggaaga agggcatcgt aaagaagaca
    57721 gaacaggcac aggcacatta agcttattcg gataccaaat gcggtttgat ctgcaaaaag
    57781 ggtttccttt gttaacaacg aaacgagtac cttttggatt gatcaaaagc gaattgcttt
    57841 ggtttttaaa aggagatacg aatatccgct atctattaca aaataaaaac catatttggg
    57901 atgaatgggc atttgaacgc tacatcaaaa gtccagacta taatggacca gatatgactg
    57961 attttggcca tagatgtttg acagatcctg aattcaatga agaatataaa aagcagagca
    58021 agcttttttg cgagaagatc ctaaccgatg attcctttgc tgaaacatac ggcgatcttg
    58081 gacacatcta cggctatcaa tggcgtcact gggaaacaaa agacggtggc tttatcgatc
    58141 aaatcaaaca agtgatcgaa gcaatcaaaa agactcctga ttctagaaga ttgatcgttt
    58201 ctgcatggaa tccggaagat gttcctagca tggctttgcc tccttgtcat acgatgtttc
    58261 aattttatgt tcaagaggga cggctaagct gccaattgta tcaacgcagt ggggatgtgt
    58321 ttctaggcgt tccgttcaac attgcaagtt atgcgttgct gactcaccta attgcacatg
    58381 aaacaggttt agcggttggc gaatttgttc acacattagg agatgctcat ctttatatca
    58441 atcatatcga acaaatgaaa gaacaattga gccgagaaat acgcgcattt ccgactttag
    58501 ttttgaatca ggaaaagcag tcagtatttg attttgagat ggaagatatc caattagaag
    58561 gatatgaccc acatccggcg atcaaagctc ctatcgctgt ttagaatcag aaaggatgag
    58621 aaaatgttag ctgcaatttg ggcacaggat gaaaacggat tgattggaaa agaagaccgt
    58681 cttccttggc gtttgccaaa tgatctgaaa ttcttcaaac aaatgacaga agcaaataca
    58741 ctagtgatgg gacgaaaaac attcgaaggg atgggtagcc gtccgttgcc gaatcgtcag
    58801 acgattgttt tgacgagaga ttcttcctat aaagcagaag gcgtgcatgt catgcatagt
    58861 gtggaagacg tgcttgctct ggaaaaagaa acagacggta tctttttcat cgctggaggg
    58921 tcagctgttt atcaagaatt tctgccattt tgtacgatat tataccgtac agtcatccat
    58981 catgtattcg atggagatgc ttattttcca cctgttgact ggtctgattg gtcactgatc
    59041 aatactagtc aaggagaaat ggatgaaaaa aatcgttatc cgcatcaatt tgaaacgtat
    59101 cagcgtaatg agaatcctgt aaaaagcggg acaggcgaat tgacgatata aaaaagctat
    59161 ggatagacta gctgttattt agagctgtcc catccatagc tttatttttt ttattaagta
    59221 aagaataaaa cagagaagta cataaaacct gcacctaaca taacaaacaa atgccaaaca
    59281 acatgcatat agcggataga attcaagcta taaaagaatg cgcctatcgt gtaagaaagc
    59341 ccgcctgcta ccatcaaaat aacaccagtc attccaagag agtgataaag aggaacagca
    59401 gccacgacac agagccaccc cataaaaata tagatgatcg tagatacttt tgatactctc
    59461 tcccgtttat gcaacgtcaa cgctttatat ataatgccaa gaatcgcaaa caaccagatt
    59521 aatacaaata aagtccaacc tagccatcct cggatactca gcaaacaaaa aggagtataa
    59581 cttcctgcaa tcaaaagaaa aatcgaacta tgatcgaata cttggaacac cttttttgct
    59641 tttgtaaaaa tcaatgaatg aaacaaagta gacgttaaaa ataaaagaat catcatcgat
    59701 ccatagatag cataagagac aatatggagg gaggaatgca agtgggcacc ttttactaaa
    59761 agaaccacta gtcccgcgat actcaatcca gtcccaattc catgggtgac tgcattaaac
    59821 acttcattta atatttcata cgttcgatta aatttcgttt tttccatgaa aacggctcct
    59881 ttactaggtg taattaatga aaaatcatta tttgtctgtt atactaaaaa tagaaattgt
    59941 cttacatgta gtttaacatg aataaggcat cttgttaaag aaagagagag agaaaataat
    60001 gacaaatgta aaaatcgtaa cagattcttc atgtacgatg gaaaaaaatg tacgcgatgc
    60061 tttaggcatt cgtgtcatgc cgctatccat catgatcgac ggaacagtat atcccgatga
    60121 tgaccaacta gacggcgaaa gatttatgga aatgatggcg aaagcaaaaa atctgcccaa
    60181 aacaagccaa ccaccaattg gagaattcat ctcgatgtat gatgagctag gagaagacgg
    60241 cagtgaagtg atttcgatcc atatgacaaa aggcttgagt ggaacagtag aagcagcaag
    60301 acaagccagt aatctaacaa aaacgaaagt aacggttatc gacagtgata ccactgacca
    60361 aggattatct tttcaggtaa ttcgagcagc tgaattagca aaagaaggta aaacagtaga
    60421 gaaaatcctt gaagaagtca aaaaagtaca agacaataca aaattataca tcggaatatc
    60481 cacactagat aacttggtaa aaggtggaag aatcagccga acaactggtt tgctgtctag
    60541 tgttttgaac atacgcgtgg tgatgaattt cgcccattct gaattaatcc ctgtaactaa
    60601 ggggcgagga aagaaaacgt tcgataaatg gtttaataat ttgaaaaatg aactgaaaga
    60661 attgccaaat gtgcgacaaa tcggtatttc acatgcggat gcaaaagaat tggcgcaaga
    60721 attcaaagag ggtttgcaag aattattccc ggacatgcat atccctgtac tgcatacgaa
    60781 tccggttatc gctacgcata ctggtaaaga cgcgtttgca atcatgtatt atgtagatta
    60841 gtcgaaaaga ggataagccc tcagcctgaa aggcttgcag cttattctct ttttctttat
    60901 aaaattagga aaataattgt tttctttttt cataaagact acctttttga taaaattttt
    60961 gataacatta gaaccaatcg aatacaagag gtgaaaaaat gaaattaacg aaacgacatt
    61021 tgatcctcgt tttgttccca ttattaattg cgatcatcat ctatgcaggt ttagctgctt
    61081 ctataccgaa agctggccca ctaatgaaac cagtaactac tgtgagagaa gatccaaata
    61141 aaaaagaaaa acttcactac atagctatcg gagattcgct gactgaagga gtaggagatc
    61201 aaaccaagca aggaggattc gttccccttg tcgcaaatga tctaaaagac cgttataatt
    61261 tgaccgcggt tgaaacagaa aactatggtg taaatggtga acgaagtgac cagatcttaa
    61321 agcgagtaaa aacaaaagaa gaaattcgaa acaatatcaa aacagctgat tttatcacac
    61381 tgactgttgg cggtaatgat ttgatgaaag ttatccaaaa taatttattc gggttaacgg
    61441 tcaattcctt taaaaagcca ctgaaaaact atcaggaaaa tgtcacagaa ctattggaag
    61501 aatttcgatc cctcaataaa accgcaccta tttacatttt aggcatctac aatccatttt
    61561 atttgaactt tcctgagatc actgacatgc agacaattgt taacaattgg aacaaaggaa
    61621 cagaaacggt gacaaaaaaa atgccgaact gctactttat cccaatcaat gatttgcttt
    61681 atcaaggatt gaatcaggaa gtaggtgtca cagaagaagg aacagatcaa acaactagca
    61741 cgacagaaaa tctaaatggc atcaaaaata atgtgctcta tgaagaagat catttccatc
    61801 caaataattt aggttatcaa ttgatggcaa atgcagtgag agatcagctg atcgagacac
    61861 aaaaccaatg gttagagaag gaggatgatc aataaacatg aaaacaagat ctgacgtatc
    61921 ttcaaacaaa acaaagaaaa caaattactg gaaattcgcc ttccttatcc ttcttggggt
    61981 agtacttggt tcgaccgtat ttttaggcag ccgtattttt gctaatcggg aaccagaact
    62041 cccagaaata ccagctctta cggaaagaca aggagatcct gtcttaacga tcaattcaaa
    62101 taaagaaaaa gtcaatcaaa tcatcagctt tttccttagt gagtatcaaa aagatagtga
    62161 tattgaatat aaattttatt tggaaaatga agcactactc aatggtactt tcgaagtact
    62221 aggttttcct atcgatttct atttatattt tgatccgtat gtgatggaaa acggaaatgt
    62281 ccaactaaaa gctagaagtc tttctatcgg aacattgagt ttgccaatca aagatgtgat
    62341 gaatatgatc aagagaaact ataaattacc agattggatc gaagtcaata cagatgatct
    62401 gactatcatg cttaggttgg atcaattccg aatgcaaaac gggatgtata ttaaagcaga
    62461 taaaatcgac ttagtaaatg atgacattcg gtttagtcta tatttgccaa aagatacctc
    62521 tgatgcttca aacaactcaa ataccaacaa aactagcacc agcagtactg aaagcaagac
    62581 aaaagaaaat aaaaaatcat cggatgagta gtattagagg gggaatcata atggaacgag
    62641 caatatttgc aggaggctgc ttctggtgta tgatccagcc tttcgacacg cttcctggta
    62701 tccatacagt gatgtcagga tatactggag ggcatgtacc agatccgact tatgaacaag
    62761 taaaaagcca aacgactgga catacagaag cagtggagat tatctatgat ccaacattga
    62821 tttcatatga acaattagta gaactttact ggcagcaaac tgatccaact gacgctttcg
    62881 gacaattcga ggatcgcggt gacaattatc gcccggtgat cttttatttg acagaagaac
    62941 agcggatcat tgctgaaaaa agtaagaaac agttagctga aagtggacgt ttcgaacagc
    63001 caatcgttac aaaaatcgaa ccagcccaac cattttatct agcagaagaa gaccatcaag
    63061 agtattataa aaagcatcct gaaaatttta aaagaaacca tgcacgccgg gctgccttta
    63121 tcgaagaaaa ttggaggaac gaagggtgaa tcgaagcttt tttcattatt taatgacact
    63181 gcgaggcggt ggcacacatg atgctttatc tgttttcgct acagaagccg gtaaagacat
    63241 ccaatttcct aagcacagct ctagctacga agaaatttct aattatctgg aactgaatgt
    63301 cgactatttg ccgtccatgg atatctttga cgaagcatgg gaaaaatatt tagaaaataa
    63361 tcatgacata taagagatag ttttgtactt tatgatcaaa aaagtttttt gtccaatagc
    63421 ctaattgaaa gaattatttt tatttcagct atctagtcgc atttttttga tatttttctt
    63481 ataataaaga aagaatgaaa aaaagaggga tggattgata gagattgaca aaaaaacaac
    63541 gctatattaa tcgcttctgg cttgtattca aatacttact tgtttctgta ttccttgcgt
    63601 tcatgttgcg gggattttta ttcatcccgg taccagtgga aggcaattca atggaaaacg
    63661 tgttgaagca aggtgatatg gttgtgatgg aaaaattttc ggagatcaga cgatttgata
    63721 ttgttgtttt tcaattagca gatggcacga tttatatcaa acgagtaatc ggtcttccag
    63781 gagaaaatgt cagctaccaa aatgatcaat tgaagatcaa tgggaaagta gttaaggaac
    63841 cttatctaac taaaaacctg aaatcagatc atgcaaatgc atcctatacg acggacttta
    63901 cactacaaga gctaacggga caatccaagc ttccggaaga taattatttt gtacttggag
    63961 ataatcgaag agtttcaaaa gatagtcgtt cgtttggcac gatcaataaa actgatattc
    64021 taggtaaagc aagatttgtc tattatccac ttgatgaaat aaaatggatc cagtgagaaa
    64081 caataaagcg tgcagtctgt tgagactgca cgctttattg tttaatccaa aatcagttta
    64141 ctaccttagt acgtaaaatc aaacagctcg tttaaacact tttctagcct ctaaagtttt
    64201 gataaatgaa taaacaacca ttatttgaat agggatttgg atcatattcc tgatgaatct
    64261 acctagaaaa attactttga aatctgcacc aaacaaaaca gacatcagcc aagaattgat
    64321 aaaaaggctg actgggatca taatcaataa ttgggcagcg atgacggaag gcaggtttat
    64381 ctttttatga tataaaaata agccattaat aaatccacca aggaaagccg tgataagaaa
    64441 agcagcttga aacgttcctg aaccaaatat cgtgaaattt aaagtgttcg acaacatttc
    64501 aagcaaacca gcccagatcg gtccatatag ataacccata agcgccatac ttaaaaatcc
    64561 gaatgtgatt tgaaaataag caggaccaat cgctatcttg tataagatga catcaagggc
    64621 taataaaaga gcagaggtcg acaacaatct tgcattcatt tcgggcaatc taaaaacgtc
    64681 ttttgcaatc aaagtgttca aaatattctc tcctttgtca gttaataata aaaggatacc
    64741 ataaaatcac caatgtgtca tgacacattg gtgatttaca tacactggca tctaacaatt
    64801 aaaaaaaggg tgagaagact gcttttataa actgtaaaaa agagattgta cgaataaaaa
    64861 aaatagaggc ggagaatact tatcgtatca tccgcctttg tttttttgtt ataaaaaacg
    64921 tcagattagt tatctgctta catcttcatc catacgggtt ctaatgtatt tccgtctaga
    64981 tcttggactt ctaatgaaaa catttcttct tcaggcattc ccatatctac ttcgtggaaa
    65041 gtaccaccat tttcttttgc aatttgagca aattttttta cttcatccgc attttcaaca
    65101 ctaaacgaaa tcaaagcacc acttgttttt ttattatcag ctatttcttt atcttttata
    65161 aattttttat aaaaatcatg agagagaagc attatccaaa aagtgtcatc ccataccatt
    65221 gaaccagctt cttcagtaga aaattcttca ttttttttaa aacccagctt ttcataaaaa
    65281 gcaacggaac gattgacatc agcaacagga aaattaacaa aaaccatact attcatttgt
    65341 ttcgtctcct ttctgttttg ttcgtctatc atctcttatt taggaaacgc tgtcaaaaat
    65401 ttagattcat atatagaaac caaaataaaa atttgcatca tcaaaaaagt gaagatgtga
    65461 tagaatgagg actggttcac tttcacttaa ctaatagatg gaatttcttt ataacgccga
    65521 ttgtaaaatt aagctagaca actaaaaaag tcatttgtgc tacactcaaa atagttccgt
    65581 ccaaagaatt ataaggagtg agtacaaatg acctataccc atcttacaac ggatgaactt
    65641 gtaatgatag agtcttattt caaaataaat caatctgttg cgaaaactgc gcatttcttg
    65701 aatcgttcaa gacaaacgat ccataaagta tacctgttct tcaagcaagg aaaatcagcc
    65761 ttagagtact atcaacagta taagaaaaac aaatcaaact gtggtagacg cccgcttgtt
    65821 ttacctgagg aacaatcaga atatattcaa agaaaggttg ttcaaggatg gacacccgat
    65881 gtgattgttg gtcgtgcagc gtttcctatt cgttgttctg ctcgtaccat ttatcgtatg
    65941 ttcaaaaagg ggctatttaa tccttctgac ttaccgatga aaggcaagcg taaaccaaat
    66001 ggacatcaag aaagacgtgg aaaacaagct ttccgtcgct ctattcatga acgtgaaaaa
    66061 gattatagcc aattctcaaa tgagtttggt caccttgaag gtgacactat cgtaggtttg
    66121 aaacataaaa gtgctgtaat taccttagtt gagcgattat caaaagttat catcacattg
    66181 aaaccgtgtg gtagacaagc gattgatatt gaaaacaaat taaatcattg gtttgaatct
    66241 gtaccgaaaa atctattcaa atccatcact tttgattgtg gaaaggaatt ttcaaattgg
    66301 aaacagatca gtaatgccaa tgatattgcc atttatttcg ctgatccagg aacgccgtct
    66361 caaagaggcc taaacgagaa ttctaacgga ttgttacgta gagatggttt attgaaatct
    66421 atggatttca attcagtaga tgaatctttt attcaatctg tcgcatcgaa acgaaataat
    66481 attcctagaa aatcactgaa ttatcgaaca cctttggaag tatttttgag ttacgtaagt
    66541 attgatgatt tgtctaactt aatttgacaa ttaagataat aaaaaagtga atttatattg
    66601 aaaaagaaaa aaacaagtaa agccggggaa tattccggta tgaaaggaat ggagagaaca
    66661 atgaccatcc aatggtttcc gggacatatg gcaaaagccc gacgagaagt ttctgaaaaa
    66721 atgaaatatg tggacatcgt atttgaattg gtagacgcac gattaccttt atcttctcgt
    66781 aatccaatgc tagatcagat cattcagcaa aaacctcgcc ttgttttatt gaataaagcg
    66841 gatctggcag atccacaaca gacaaagctg tggcaagaat attttcgtaa acaaggccat
    66901 cttgcattag ctatcacggc acaagaaagt aaaggaatta aaggtctagt acctgcggct
    66961 aaagaagcat tgaaagaaaa acgggcaaga gatgctgctc gggggatcaa gcctcgcgct
    67021 attcgggcaa tggtcttagg tattccaaat gttgggaaat ctacgttgat gaatcgacta
    67081 gtaggcaaga aaattgcaca gacaggaaat aagcctggcg taacaaaagg tcagcagtgg
    67141 cttcgtttag gtaatgagtt ggaattgtta gacacaccag ggattttatg gcccaaattt
    67201 gaagatgaag aaatcggcaa aaaactagcg ctgacaggcg cgatcaaaga ccagcttgtc
    67261 catttagatg atttagcgat ttacggatta gacttttttg cccgatatta ttcaggaaga
    67321 atcagcgatc gttatggttt atcaatcgag caagaagaaa aattggcacc ggaattactg
    67381 atggatatta cagcaaaacg tgggtataaa gaagactatg atcgtggcag cgaattagtc
    67441 gtgcatgata ttcgtcaagg aaaacttgga cgatacacac tggatcgttg cgaagaaatg
    67501 gagcaaaaaa gtgatgactg aatccatcca aagtatcaaa gaacgattga aaagcataca
    67561 gagaatagat gatcctacac tagaatcatt aagaaatgat ccacgcaaag gcgtgcagca
    67621 agcactggct tcttttgaac gacgattgaa aaaggaagca ttaattaagg aaaaatatga
    67681 agaaatgttt tgctttgaaa agcaagggta cgcacaaggt caccaatgga ttgccggcat
    67741 tgatgaagta ggaagaggac cattagctgg acctgtcgtg gcagctgcag ttatcttgcc
    67801 gaaaaatgag atcatattag ggctgaatga ctccaaacaa ttatcagaga aaaagcgaga
    67861 acttctttat caacaagttc aagaaaaagc cctggcaatc gggatcggga tcgttgatca
    67921 gcagacaatc gatgatgtga atatttatga agcaagtaaa ttagcgatga tccaagcagt
    67981 agaaaaattg tccattgtac caagctattt gttgatcgat gcgatgacct tggatttgcc
    68041 tattcagcag gaaaaaataa tcaaagggga tgcacgatct gtgtcgattg ctgctgcaag
    68101 tatcattgct aaagtatatc gagaccaatt gatgaaagaa tatgacgtgc tttatcctgg
    68161 atatggtttc tcgcataatg caggttatgg aacaaaggaa cacttagaag gacttgaacg
    68221 acaaggaatt acgccaatcc atcgtaaaag ttttgcacct gtaaaaaaat atttacaata
    68281 aaaaacagcc gcttttacgt atcttattag aaagagagac gaaaggcggt tttatgatgt
    68341 atcaaataga agaaaattta ttgaaacttg cttatacaaa aggaatcagt agtcaaggaa
    68401 aatgggcact tgctaactgg atgatgcagt atccttataa agaaatcgga tttgaagaca
    68461 tcattaagat aggtaaaata accaaacacc gggagctgtt tcgcaaaagt tggcaggaaa
    68521 ttcacgctaa ctggggcaaa atccaatcga aacagtcgtt tatcacttgc tttgatccca
    68581 aatatccccc tcagctgctt cacttgacct atccgccaat tgtactgttt tataatggtg
    68641 atctatcttt actttcttgt aacatgttaa gcgttgttgg tggaagacac acttctgctt
    68701 tagccaaaaa aacagtttat tatttactaa aaccagtgat agaagctgga tatgtgatcg
    68761 tcagtggctg cgcgaaaggg atagatacat acggtcacca agcagcaatc tctcatggtg
    68821 gacggacaat cgcagtcatt ggaacaggga tagagcaagt ctatcctgct gaaaatcgtg
    68881 acttgcaaga ggtgattgca aatgatcatc ttcttttgag tgaatatcct cctcaatccg
    68941 gcccgaaaaa gcattgtttt ccaatgagaa atcggattat cgcagctttg tctaaaggaa
    69001 catgtgtctt tgaagccaag aaaaaaagcg gctctctgat aacagcacag caagcattgg
    69061 atcttggaag aacagttttt tctgttccag gaaatatttt aacagaacat tctaccggat
    69121 gtcatcattt gatccaagac ggtgcagttt gcacggtttc gggtgaagat atcttggcag
    69181 aatttaaaga ataacaccat acttttcatt tgcttttctt gacaaacgtt ttctgaatgg
    69241 atatgattag ctacgatttg tatgaatcct cttttaatag taggaacacg acaatggaaa
    69301 ggagtccatt cagtaatggc gtataaatat ttagtgattg tagaatcacc tgcaaaagcc
    69361 aagacaattg agaaatatct tggacgaaac tataaagtaa tggccagtgt tggccatata
    69421 agagatttac cgaaaagtaa aatgggtatc gattttgaaa acaattatga accccattat
    69481 atttctatac gcggaaaagg cgatgtcatc aaaagcctga aagccgcagc aaaaaaagct
    69541 caaaaagttt acttggcaag tgacccggat agagaaggag aagcgattgc ttggcattta
    69601 gcgtacctac ttgggttgga tctgaaagaa aaaaatcggg tggtcttcaa tgaaatcaca
    69661 aaagacgcag tcaaagcagc ttttaaggaa ccaagaacga tcgatgtaga tttagtagat
    69721 gcacagcaag ctcgtcgtac cttagacaga atcgttggtt attcgatcag tcctattctc
    69781 tggcgtaagg tcaagaaagg gttaagtgca ggacgtgtcc aatctgtcgc tttaaaaatt
    69841 attattgacc gtgaaaaaga gatccgagaa tttgttccag aagaatattg gagcatcgac
    69901 ggtaatttta aaaaagctcg caagaaattc aaagcaaatt tctggggaat cgacggtaag
    69961 aaaaagaaat taccagatgc acaaagtgta aaagaagtca ctgctagaat tgatggaaca
    70021 gacttcgaag ttaaaaaagt agaaaagaaa gagcgcaaaa gaaaccctgc gaatcctttc
    70081 actacaagta gcctgcaaca agaagctgcc cgtaagttaa actttcgaac aagaaaaacc
    70141 atgatggtag cacagcaatt atatgaagga atcactttgg gtaaacaagg aacagtcggc
    70201 ttgattacat acatgcgtac tgattctacc cggattgccg atacagcaaa agcagaggcc
    70261 gcagcattta ttgaagatac gtatggaaaa gaattttcta gacatggtca ccgaaagatc
    70321 aaaaatgctc aaggcgcaca agatgcccat gaagctgtgc gtccaacgag tgtgcttcgt
    70381 acaccagatg aactcaaaca atatttagat aaagatcaat tgaaacttta tacattgatc
    70441 tggtcacgtt ttgtggctag tcagatgaca ccagctattt tagatacaat gaaagtgaca
    70501 ttagaacaaa atggtgtcat cttcattgcg aacggttcaa aaatcaaatt caaaggattc
    70561 atgcaagtgt atgtggaagg tagagatgac ggcaaagaag agaaagaaaa tattcttcca
    70621 gaacttgaag aaggcgatgt agtccaatct gttgatattg aaccaaaaca acattttact
    70681 caaccaccag cacgttttag tgaggctacg ttgattcgtg cactagaaga aaacggggta
    70741 gggcgacctt ccacttatgc accaacactt gatacgatcc aaagacgcta ctatgtaaaa
    70801 ttgacacaaa aaagatttga gccgactgaa ctaggagaaa ttgtcaattc attaatctgt
    70861 gaattcttcc ctcaaatcgt cgatatccat ttcacagcag aaatggaagg agatttggat
    70921 aaaattgaag aaggcacgga agcgtgggtc aaagtggttg atcgtttcta taaaccgttt
    70981 gaaaaagaac tgacgaatgc ggaagaaaaa atcgaaaaaa ttcaaatcaa agatgaacca
    71041 gcaggattcg attgtgatgt ctgtggacat ccgatggtga taaaattagg gaaatatggg
    71101 aagttttatg cttgcagtaa ttttcctgat tgtcggaata cgaaaccaat cgtaaaagaa
    71161 atcggtgtga cttgcccagt atgtcatgaa ggacaagtca ttgaacgaaa atctaagaaa
    71221 aacagactat tctatggttg ttcccgtttc ccagaatgtg aattcacttc ttgggataaa
    71281 ccagtgggca gaaattgtcc aaaatgtgac ggatatctgg tcgagaagaa agtcaaaggc
    71341 ggtaaacaag tagtctgtgt aaatggagat tacgaagaag acatccaaaa ataatagaaa
    71401 agaggttctt tcattgaaaa tggtaaatgt tgttggagca gggcttgccg gaagtgaagc
    71461 tgcttggcag atcgcacaag caggagtccc tgtccgttta tatgaaatgc gtccagtcaa
    71521 atcgactgag gcgcatcaca caagtaattt tgctgaacta gtatgttcca attcattaag
    71581 aggaaacagt ttggctaatg cagtagggtt attgaaagaa gaaatgcgcc gattgaattc
    71641 ggtagtaatc acttcggcag atgaaacagc agtgccagct ggtggtgcct tagctgttga
    71701 ccgcgattct ttttctgaaa caattacccg aaaagtcaaa gagcatccat tagtaacagt
    71761 gataaacgaa gaaattactg aaataccgga aggaatcacc gtagtagcaa caggaccttt
    71821 gacttctcat ccattagcag aaagcatcaa agcattcaat ggttcagatg gtttttattt
    71881 ctatgatgca gctgcaccaa tcgtagacaa agctacgatc gatatggata aagtatattt
    71941 gaagtcccgc tatgacaaag gagaagcagc ttacttgaac tgtccgatga cagaagagga
    72001 attcaaagct ttccatgagg cactaatcaa tgccgaagtg gcagaactta aatcttttga
    72061 gaaagaaaaa tattttgaag gatgtatgcc aattgaagta atggcacagc gaggattcaa
    72121 aacgatgctt ttcggaccta tgaaaccagt tggattggaa gatcctaaaa ctggcaaacg
    72181 tccttatgct gttatccagc tgcgtcaaga caatgctgca gcttctttat acaacatcgt
    72241 gggctttcag actcacttga aatggggcga gcaaaaacgt gttttccgga tgattcctgg
    72301 gttagaaaat gctgaattcg ttcgctatgg tgtgatgcac cgaaacagct ttatgaattc
    72361 gccagaattg ctgaaaccga cttaccaatc aacgaaacgc gaagatctgt tctttgcagg
    72421 tcagatgact ggtgtagaag gctatgttga aagtgcagcg agcggactac ttgctggaat
    72481 caacgccgca cgcttagcaa aagaaatgga accagtcgag tttccacaag aaacagctat
    72541 tggaagtatg gcttactaca ttactcatgc ggaaggaaaa catttccaac cgatgaatgc
    72601 caactatggt ctgtttcctg aactgccaga gcgcattcga gacaaaaaag caagatatga
    72661 agcaattgca caacgagctt taaaagctaa tgaggcaata aaaacagaat tagaaaaaac
    72721 tgcaatccca aatgagtaag gaaactccat gaagagggtg agacggatag tctcgctctt
    72781 tttcttgcgt ttttcttaag aatttctcaa acaaaatgtg aggaattgcg ccaattgtga
    72841 acaaacttag aaatattggt taaattctga caatttattt gtctttttat aaacgctgtg
    72901 ctaaattatt ctctgggaca aaaggaaggt gagaaaatga cagaagccac gtgggaacaa
    72961 aaatttttgc gttatttgat tgtcgaaaga ggatattcag aaaaaacgaa agaagcatat
    73021 gaagaagaca ttctccattt caaacatttt ttggaggaat ctggagacgc tgatctgctg
    73081 aaggcggaat attttacggt tcgtgtctat ttgagtgcat tagctgatca tcgtcctagc
    73141 tatagcagaa attctatcag tcgcaagata gccagcctac gttcgtttta tcagtatctt
    73201 ctcaaagaag aagtgataaa agaaaatcca ttttcttacg ttcatctcaa aaagaaaaat
    73261 ttgagactgc cacgtttttt ttatgaaaat gaaatgcaag cactttttga cagcgtagca
    73321 ggagatagtc ctttggattt gaggaaccgc gctttactgg aagtccttta cgggtcaggc
    73381 atccgtttaa gtgagtgtag cagtttgtcg gtatcagaca ttgattttga ttcagaggtc
    73441 atgttgatcc atggtaaagg gaacaaagag agatatgctc ctctaggttc ttttgctcaa
    73501 gatgctctgc aagaatattt cagggaaggc cgccaggtat tgatgacaaa atatcacgaa
    73561 gagcatgata tcgtttttgt gaatcatcat ggaaaaccaa tcacgcctac tggaattgaa
    73621 tatgtcttga atcaaatgat caaaaaaagc agtttggata gtgatatcca tcctcacatg
    73681 ctgcgccata catttgctac tcatttatta aataatgggg cagatttgcg gacagtccaa
    73741 gaattgctag gacatgccaa tttatcgact acccagatct atgcccatgt aacaaaagaa
    73801 agtttacaaa aaaactatcg ttctttccat ccaagagcgt aaaaataagg aaaagaacaa
    73861 atttaggagg aaacaaacat ggtcgaatca ccatttcatt caaccactat ctgtgccgtc
    73921 gaaaaagacg ggaaatttgc tatggctgga gatggccaag taacaatggg tgaatctgtt
    73981 gtcatgaaag gcactgcaaa gaaagtacga cgtatctaca atggagaagt tgttgtaggt
    74041 ttcgccggaa gtgttgcaga tgcgtttact ttagaagaaa aatttgaagg caaactaaac
    74101 gaatataacg ggaatctaca acgcgcagct gtcgaattag ctcaagaatg gcggacacag
    74161 caatctatgc aaaaacttga agcgatgctg atcgtaatga atgataaaga aatgcttctt
    74221 gtttcaggta caggagaagt tatcacacca gatgatggta tcttagctat cggatcagga
    74281 gggaattttg cgttggctgc agcccgagca atgaaaaact atgctcaaaa cgacatgagt
    74341 gcgaaagaaa ttgcagaaaa tgcgttaaat attgccgcag atatctgtat ctttactaat
    74401 cataatatta tcgtagaaga actgtaagag gtgagtagat atgtatgaat taaaacaaac
    74461 accaagacaa atcgtaagtg aattagatga atatatcgta ggacagcacg cagcaaaaaa
    74521 atctgttgcc gttgctttgc gtaaccgcta tcgccgtttg cagttagatg aaaaaatgca
    74581 acaagatatt acaccaaaaa atatgctgat gatcggacct acaggggtcg gtaaaacaga
    74641 aatcgcacga cgtttggcaa aaatcgtcaa tgcaccattt gtcaaagtcg aagcaacaaa
    74701 atttactgaa gtcggttatg tcggaagaga tgtagaatca atggttcggg atttagtaga
    74761 aaatgcgatc caaatcgtag aaaaagagca gtacagccgt gtatacacaa gtgctaaaaa
    74821 aaatgcagaa aaacgtttgg taaaaatctt ggtacctggt atcaaaaaag aacagaaaca
    74881 atccactaat caatttgaac aaatgatgaa tctttttaat gctagtcagc aaaataccgc
    74941 tgaaccgcaa gaagaagtga cagaagatat tcgaatcaat cgccgtacag tcttggaaca
    75001 actacaaaag ggtttactgg atagtcgtga agtaaccatt gaagttgaag accaaaagaa
    75061 acctgcacca atgatgaaca atggattgga gcaaatgggg atcgacctaa atgaaactct
    75121 aggtgctttg acaccgaaga aaaaagtgga acggacggta acagtcaaag aagctttaga
    75181 gttattgatc aaggaagaat cagcaaaatt agtcaaggat gctgatatcc atagtgaggc
    75241 aatcaaatta gcggaagaaa gtggaattat ttttatcgat gaaatcgata agatcacttc
    75301 taaatcacag caatctggag aagtatccag agaaggcgtc caaagagaca tattgccaat
    75361 tgtcgaaggt tcacaagtaa acacaaaata cgggatcatc caaacagatc acgtcctatt
    75421 cattgcttca ggagctttcc atttgtctaa accaagtgat ctaattccag aacttcaagg
    75481 acgcttcccg atacgggtag aattagatga tttgacagct gaagattttg tgaagattct
    75541 gaccgaacca aataatgctt tagtaaaaca atacgtcgcg ttgatcggta cagaaaatgt
    75601 gtcagttacg tttacaaaag aagcaattga acggatcgct caaatcgctt tcgatgtcaa
    75661 ccgtgacaca gataatatcg gcgcacgcag actgcatacc attttggaaa aactactaga
    75721 agatctatta tttgaagcac cagatatgca aatgggagaa ataacgatca cagaagcata
    75781 tgtggatgac aaacttggct caattgcggc aaatgaagat ttaagtcgct atattttata
    75841 aaaaatcgga ggacgacaat gacagacttg ctaacgaaaa cccgcaagat aaacacactt
    75901 ttgcagcaaa agaatacttt tgacatgcaa gcagatttgc cttacgataa aatggctgtc
    75961 acactaggtg gtattcttga cagcaacacc tatatcatag gaagtgacgg agtattacta
    76021 ggttataatg aacgtcacga tgtgaacaat gaacgaatca aaacaatgtt tgttgagaaa
    76081 caatttcctg aatcttatac tgatacagtg gcacagttga tgaaaactga agctaatatc
    76141 ccaattacta gtgatattac tgcatttccg gtagagtcaa gagagaaata tccatttggt
    76201 ctgacgactg tcgttccgat ctttggagct ggcgaacgtt tgggtacgat tatcttatct
    76261 cgtatggaac aatcgtttgc agatgacgat ctagtcttag cagaatatgg agctacggtt
    76321 gtcggtatgc agatccttta tcagaagtct cgaagcatcg aagcagatgt acggagtgca
    76381 acggctgtcc aaatggcaat caacacatta tcttatagcg aattgaaggc tgtacaagct
    76441 atctttgaag cattggatgg agatgaaggt cgtctgactg cttctaatat tgccgatagt
    76501 atcggcatca ctcgttctgt gatcgtcaat gctttacgaa aattggaatc agctggaatc
    76561 attgaatctc gttcgttagg catgaaggga acttatttaa aagtattgaa ttcccgtttt
    76621 aaagacgagt tagcaaaaca cagttattag aagtaagggg actggggcag atagaaatgt
    76681 ttgttccagc cttcccattt taaaaagtaa gtcgaagttg gaggaatggc aatgactgtt
    76741 attattcaaa atgaccagct gatcgccgaa atcgctgaac acggtgcgga attgatcagt
    76801 ctgaaatcaa aagaacatgg tttagagtat atctggcaag gtgatccagc gtattggggc
    76861 agacatgcac cggttttatt cccttttgtt ggacgtttaa aagatgatca gtatgtttat
    76921 caagggaaaa catatcaaat ggggcaacac ggatttgctc gggatatgga ttttgaagtc
    76981 attgaacatg gacaagaaat ggctagtttc atcttaagga gtacgccgga gacattagaa
    77041 aagtatccat ttgattttga attagtgatc agctatgaat taggaggaga cggcatcacg
    77101 gttcattatc aagtggcaaa taccggtgaa aaagaaatgt tcttttctat cggtggacat
    77161 cctgctttca atgtacctct tgaagaagga ttgtcatttg aagatttcta tctggcattt
    77221 tctccattga aatcaagatt acgcctacca ttgaaaggcc cattcatcga tatggatcaa
    77281 aagacgttag gtcagacaaa tacgaatctg gctttgaatc gtgaactttt tgagcaagat
    77341 gccttggttt ttgaaacaag gggtctaaat gcgtacgcca ttcgaagtga aaaatcttcc
    77401 catagcgtaa ctttaagcta taaagatatg ccttatgtag ggatctggtc tccttatcca
    77461 aaagaagcgc ctttcgtttg tatcgaaccg tggtgcggaa tcgctgacac ggtgaacagt
    77521 agtggaaact tgatggaaaa agtagggatc aatcaactag gtgcccatga aatcttcaag
    77581 acaaaatatt ccatcacagt gaagtaagag gttgtgaccg agaagttagc agggaacacc
    77641 gtttaccaag gtgtgtaaaa aagctatcct gtaagtaata agggagtgtg acatcgattt
    77701 gtcacactcc cgttttatct ctttggaatg aaaaagtatc aggatttttt cttgctgcta
    77761 ttcaaaccaa acgggattcg actttcagta tggtttctga tacgcttgat attgtcttta
    77821 tgccgataaa agataaagga agaaactaaa aaggcaatga tcgtcagtaa ccaattgggc
    77881 ttagccaaaa tcacaggagc tacataagga aggacgatcg tagataaagt gatcaatact
    77941 gcactgatca tactgctcaa actcaccata cttgtacaaa ataaactaac gataaagatg
    78001 atacaagaat aaacaaaaaa tacaggatta aatcctaaaa gcattcctgc acttgtcgca
    78061 acagcttttc ctcctttgaa cccagcaaaa atcggaaatg tatggccaag aatggcgcat
    78121 accccaaacc aaagagggtt aatcccagaa aggtggaaga tcatcggcaa acaagtagcc
    78181 aatgtacctt ttaaaatatc cataagcagg acaacaatcc cggctgtttt gcctaataca
    78241 cgaaatgtat tggtcgttcc agtattaccg ctcccgtgtt gtcggatatc ttttttgaaa
    78301 aacaatttgc caatccagac accggaagga atagaaccta aaagatatgc taaaataagt
    78361 aataagatca atttcatttt ttaccctctt tcaagttgaa aactatcctt tattttagca
    78421 tgaataggta acaggaacaa ttgaaaaaat gaaagataca agtataatag atggaataaa
    78481 aagaaaagca aggggtggca gaaatgtctg taaaaaatcc aacacaagaa acgattttca
    78541 actatttgga agaagcaaaa aatattgccg tcgtaggctt gagcaataaa ccagaacgaa
    78601 caagttacat ggttgcacag gtattgcaag acaatggata taaaatcata ccagtaaatc
    78661 cattattggc tggtgaagaa attctaggcg agaaagtcta tgcgaatttg acagaaatcg
    78721 atgatccaat cgatatcgta gatgtattta gacggagtga atttttacct gaaatagcag
    78781 gtgatttttt gaaaactgat gctaaagtgt tctgggcaca attagggatt gaaaacaaag
    78841 aagcagctga gatgctaaga aaagcaggac gtacagatat cgtaatggat cgttgtatta
    78901 agattgaact tggtaagttg aaagaaaacc aaaaataaaa atgaccacaa agaagagatg
    78961 atcgatagag gggaatgtcg aaaaagcggt taggagataa ttaaaattga tttctcgtct
    79021 accctctttt ttttcacgac cttttctagt atgataatcg tggtgcggtt ttgccgcaaa
    79081 gtattattga aggagcgttt gccttggcaa aaaaagtaaa caacgaatac aatgactcgt
    79141 ccatccaagt attggaagga ttagaggcag ttagaaaaag accagggatg tacattggat
    79201 ccactgacag ccgaggcttg catcatttag tctatgaaat cgtcgataat gcagttgacg
    79261 aagcattatc aggttatggc agtgagattg atgtgaccat ccatgaagac aatagtataa
    79321 ctgttgcaga tagcggacgt gggatgcctg taggaatgca tgcttccgga ataccaacag
    79381 ttgaagtgat ttttactgtg ctgcatgctg gcggaaagtt tggtcaagga gggtacaaaa
    79441 cttctggtgg actgcacggt gtaggagcca gtgtggtaaa tgccttatcg aaatggctaa
    79501 ttgtaacgat cgttagagat ggcgtagagt atcagcagaa atttaaaaac ggcggtaaac
    79561 ctgacggtac tttgaaaaag ataggaaaaa caaaaaaagc aaacgggacg actgtccatt
    79621 ttttaccaga tgatacaata ttcagtacaa cgaaattttc atatgaaatt ttagccgaac
    79681 ggttaagaga atctgccttt cttctaaaag gcgttaaaat ctctctttct gatcttcgtg
    79741 gagaagaacc agtcaaggaa atttttcatt acgaagaagg aatcaaagaa tttgttgatt
    79801 atctgaatga agaaaaagat acattgacac cagttgttta tttttcagga gaaaaagaag
    79861 gtatcgaagt agaggtcgct tatcaataca atgacggtta ttcagaaaat gttttatctt
    79921 ttgtcaataa cgttcggaca aaagatgggg gaacccatga agctggtatg aaagcggcga
    79981 tgacgaaatc ttataatgaa tatgcgagaa aagttggttt gctgaaagaa cgggacaaga
    80041 atctggaagg tagtgatttc agagaagggt tagctgctgt actttctatc cgtgtacctg
    80101 aaaatcttct gcaatttgaa gggcaaacaa aagaaaaact tggtacgcca gttgctcgta
    80161 ccgtagtaga taacgtcatc agtgaacaaa tgggctttta tttgcaggaa aatagcgaaa
    80221 tgagccagat gttggtccgt aaagcaatca aagccagaga agcgagagaa gcagcacgta
    80281 aagccagaga agaaagccga aatgggaaga agcgtaaaaa aggcgaatct cttctttcag
    80341 gaaaattgac acccgcacaa tcccgaaacc ctaagaaaaa tgaattgtat ttagtcgaag
    80401 gggattctgc tggcggatct gctaaacaag gaagagacag aaagttccaa gcaattctac
    80461 ctttacgagg aaaagtcatc aataccgaaa aagctaaaat gcaagatatc ttgaaaaatg
    80521 aagaaatcaa tacgatgatc tatacgattg gtgcaggagt cggtcctgag ttttctatcg
    80581 aagactgtaa ttacgacaaa gtaatcatca tgaccgatgc ggatacagac ggcgcccata
    80641 ttcaagtatt actgcttact ttcttttatc gttatatgaa accgctgatt gaagctggga
    80701 aagtatatat cgcgttaccg cctttatata aagtatccaa aggacaagga aagaagcaag
    80761 tcattgaata tgcgtggaca gatgacgaat tggcagcaat gattaaaaaa gtcggcaaag
    80821 gatatatgct gcagcgatat aaaggacttg gagagatgaa tgcggaacaa ttgtgggaaa
    80881 caacgatgga tccgacttcc cgtacgttga tccgcgtccg tatcgatgat gctgcgcaag
    80941 ctgaacgtcg agtaaccaca ctgatgggtg acaaagtaga acctcgaaga aaatggatcg
    81001 aaaatcacgt gcaatttacg ctcgaagaag atggcagtat cttagataaa aaagaggata
    81061 ctgaaatctc tccttcagtt tctaatgatt tattagatga agaacgagca gataaaaatg
    81121 aaaacaatca gttatttgaa gtagaatagg aggtcacctg atggaaaatc gacaagaagt
    81181 acaagaatta actttagaag aagtgatggg tgaccgcttc ggacgttatt ccaaatatat
    81241 tatccaagaa cgtgcactgc ctgatatccg tgatggattg aagccagtgc aacgccggat
    81301 attatttgcc atgaacaaag atggcaatac gtatgacaaa ggattccgta aatcagcaaa
    81361 atctgtcgga aatatcatgg ggaattatca tcctcatggt gacatcagta tttatgaagc
    81421 gatggtccgc atgagccaag actggaagtt gcgggaagta ctgatcgaaa tgcacgggaa
    81481 taacgggagt atggacggtg atccacctgc tgcgatgcgt tatactgaag ccagattatc
    81541 caagttaagt ggagagatgc tagcagatat cgagaaagaa acagtcgatc tcgtgtggaa
    81601 ttttgatgat acagaaaaag aaccaacagt ccttccagct cgatacccaa accttctcgt
    81661 caatggatcg acaggtatat ctgctggata tgcaacagaa atcccgactc ataacttagc
    81721 tgaagtgatc gatggcacaa tctatatgat cgatcatcca caagcgagcc ttgaaaaatt
    81781 gatggaatat attcctggac ctgactttcc aacaggaggg atcttgcaag gaaaagatga
    81841 aatcaaaaaa gcttatgaaa caggacgagg aaaagttatt cttcgttcga aaacaaagat
    81901 agaggcaatg aagggtaata aacagcaaat cgtgatcaca gaaatcccat atgaagtaaa
    81961 taaagcgacc ttggtgaaaa aaatggatga gatccgtttg aataagaaaa tcgatggaat
    82021 agctgaagta cgagatgaaa gtgatagaac aggtctgcaa attgtcgtcg aattgaaaaa
    82081 agacgccaat gctcaaggaa ttttgaatta tttgttcaaa aacactgagc tacagatcaa
    82141 ttacaatttc aatatggtcg ctatcgacca tatgactccg catcaagttg gactgaaaga
    82201 cattttaaga agttatatcg agcatcgaaa gcaagtcata acaaaacgta gtcaattcga
    82261 tttagccaaa gctcaaaaaa gacagcatat cgtagaagga ttgatgaaag ctttatcgat
    82321 tctggatgaa gtaatcgcaa cgattcgtga aagtaaagac aaaaaagacg ccaaaaagaa
    82381 tttagtcgac gtctttcaat ttacagaaga acaagcagaa gcaattgtca cattgcaatt
    82441 ataccgtttg acgaacacgg atatcacaga gcttcaaaag gaatccgaaa gcttgattgc
    82501 gcaaatcaca gagttgaata aaatcctttc aaatgacaaa gagcttttct ctgttatgaa
    82561 aaaagaattg cgagaagtga aaaaaaatta ttcttccgct cgtctaacga caatcgaaga
    82621 cgaaatcgaa gaaatcaaaa ttgatacgca agtcttagtg gcacaagaag acgtaattgt
    82681 cagtgtgacg cgagaaggct atgtgaagcg aacaagcctt cgttcttata gtgcttccaa
    82741 accagaagaa atcgggatgc gagaaggcga ctacttgcta tactcaggtg aattgagtac
    82801 attggaccat gttcttctga tcacaaataa agcaaatgtg atctaccgcc ctgtccatga
    82861 actacccgat ttgaaatgga aagatgctgg tgagcacatt tctcaaacaa tctccaatct
    82921 gtctgtagac gaatcgattt tagctgtttt cccatatcaa aaaattcaag cagagaaaac
    82981 atttgtattc atcagcaaga atggattgat caagcaaaca cgaatgacag agttcgaacc
    83041 atggcgcacc tataagagcc gtccgctcag ctgtatgaag ctaaaggcgg aagaagatga
    83101 actgattgct gtgtatctga aagaagatca gccaggctta gatgttttct tagtaacgca
    83161 tcaaggaatg ggtttgcgct atcctttaac agatgttccc gttgttggag caaaggcagc
    83221 tggagtgaag tctatgaatt tgaaagatgg agataggatt atcaacgggc tgcttgtgtt
    83281 agcagaaggt gatacgccga tcgtaatcgt tactcagcga ggagctgtta aacgcatgct
    83341 tgctcaagaa atcagccaaa cttcacgtgc gaagcgggga gtgacggtca tgcgtgagct
    83401 gaaaaaaaat ccgcaccgta ttatctatat gtcggaagga agatcaaaag aaataacctt
    83461 gatgaatcaa aaagggcagc agttaacgat tgacccaact gatttcccta taggtgatcg
    83521 aacatcgaat ggatcatttg cgatggatga gaaaaaaggt ggagaagtca tcgaagttat
    83581 cgatgctcct gagattttca tcgagtcgta actgttgtag tattttaatg aagagacgcc
    83641 atctgtatta atacaggtgg cgtctctttt tcattcattt ttaataaatc cttatacgtt
    83701 tgcaaaaaaa gatgaaagtg ttcacaatgc ttaaatgttg gtatgtcaac gttgatttaa
    83761 atatatgtaa ataaaacata aaaattgtta aataaaaaac aagaaaaccc ttgcaaagat
    83821 agggtgctgt gttatactaa tcacgtaaaa actgttacat aaaacagaaa tttaggaggt
    83881 ccatacaatg gagcaatgga atggatttaa aggtaccaaa tggaaagaag gcgtagacac
    83941 tagagacttt attcaaaata actacactga atatcgcggt gatgacagct ttttggaagg
    84001 cccaacagaa gcaacagaaa aattatggga tgaattgcaa aagttaaatg atatccaatt
    84061 cgagcgtaac ggtgtctatg atatggacac agaaatcgta tcaactatta cttcacacga
    84121 tgcaggctat ttggataaag atttggaaaa aatcgtcggt ctacagacag acaaaccttt
    84181 gaaacaagca ttcatgccgt tcggcggaat caatatggct aacaatgcgc taaaaagcaa
    84241 tggctataca ccaaacgatg aattgacaca tattttcact gactggcgca aaacacataa
    84301 tcaaggtgta tttgacgctt atactgctga aatgcgtgct gctcgtaaaa acaaaatcat
    84361 tacagggctt ccagacgctt acggtcgcgg acgtatcatc ggtgactatc gtcgtgtggc
    84421 attgtatgga atcgactatt tgatggaaca aaagaaaaaa gatcatgata tgactggtta
    84481 tcatacaatg agtgaagaag tgattcgttt acgtgaagaa atcactgaac aatatcgtgc
    84541 attagcgcaa atgaaagaaa tggctgcgaa atatggctat gatatttcta aaccagctgc
    84601 aaatgcaaaa gaagctgtcc aatggctata cttcggatat ttagcagcaa tcaaatcaca
    84661 aaatggtgct gcgatgtcaa tcggacgtat ctcagcattc cttgatatct atatccaacg
    84721 tgatttggat aatggagtaa tcacagaaaa agaagcacaa gaattgatcg accacttagt
    84781 aatgaaatta cgtatggtca aattcgcgcg tacacctgaa tataaccaat tattctcagg
    84841 attccctatc tgggctactt tatccattgc aggtatggga ttagatggtc gttcattagt
    84901 tacaaagaat gatttccgtc tgcttcatac attaacaaat atgggacctt caccagaacc
    84961 aaacttgact gttttgtatt ctgaacacct tccagaagga ttcagaacat atgctagcag
    85021 aatcgctatc gaaagttctt cgattcaatt cgaaaatgat aatcagcttc gtgaacaatg
    85081 gggttctgat gattgtgcga ttgcttgctg tgtatcagga acagttgtcg gaaaagacat
    85141 gcaattcttt ggcgctcgtg ctaacttggc taaaacacta ttatatgcca tcaatggcgg
    85201 agtcgatgaa gtgactggcg cccaagttgg accagaatat cgtccaatca catcagaaat
    85261 actagacttt gaagaagtga aacataactt catgaatatg atggattggt tagcagaact
    85321 atatgtcaat acgttgaaca tcattcatta tatgcatgat aaatattctt atgaagcagc
    85381 tcagctggct ttgatggatt ctgacttgca acgcacattt gctacaggta ttgctgggat
    85441 ctctcatgcg gcagacagct tgtcagcgat caaatatgca aaagtaaaag caatccgtaa
    85501 tgaaaaagga atagcagttg attttgaagt ggatggcgac ttccctaaat acggaaatga
    85561 tgatgaccgt gcagatgaat tagtttcatg gatcttggaa tacttcatgg gacaaatcaa
    85621 acgtcataaa acttaccgca actcaaaacc aacaacttca ttattgacga ttacttcaaa
    85681 tgttgtttac ggtaaaaata caggaaatac tcctgatggc cgtgaagctg gaaaaccact
    85741 tgcgccagga gcaaatccaa gttacaatgc ggaacaaagt gggttattag ctagcctgaa
    85801 ctcaacagct aaaatgaatt acggctgtgc attggatgga atttcaaata cccaaacaat
    85861 caatccttca ggtcttggaa aagaacaaga tacaagaatc gaaaacttgc gtaatgtctt
    85921 agatggatac ttctcaaaag gagcttatca tttgaatgtc aatgtattca gtaatgattt
    85981 attgcgtgat gcaatggaaa atccaatgaa atatccgaac ttgacgatcc gggtatctgg
    86041 ttatgcagtt aaattccgtg acttgacaag agaacaacaa ttagatgtct tgatgagaac
    86101 ttcacacgac agaatgtaaa aagttcgtga aaaaagtttt tcgacttagc gtcgaaagga
    86161 cgttgaatag ccatcgcatc aaaatcgaat aatcagtatg cttatttgaa agaaacgatt
    86221 tacaggggca ggacttgtat gttccgtccc tgtaaatttt acacataacg aagcggcaat
    86281 tgcgaaaaat tttgttcaga aagcttcgct tcgacagcta acttgtactt aaaatgaaat
    86341 caaagaaaag atgtgaactt aaatggaaga aaaaacaatc ggctatgttc attctatcga
    86401 aactttcggt tcagtagacg gacctggact gcgctttgtc gtgtttatgc aaggatgccg
    86461 catgcgttgc caattttgtc acaatccaga tacttggaat attggcggag ggaaagaata
    86521 cacagcagat gaacttttag ataaagcaga acgcttcaga ccttactggg gagataaagg
    86581 tggtattact gttagtggtg gagaacctct attgcagatc gattttttga tcgaactatt
    86641 taaaaaagca aaagagcgga agatgcatac tacattggat acatgcggaa aaccttttac
    86701 gtatgaagaa cctttctttt cacgttttca agaattgatg aagtatacag atttactgtt
    86761 atttgacatc aaacacatcg ataatgaaga gcataaaaaa ctaacgcatt gggacaatga
    86821 aaatatttta gaaatggctc aatatttgtc aaaaatcaac aaaccggttt ggattcgcca
    86881 tgtactagtt cctgagcgaa gtgattatga tgaatatttg atccgactag acaactttat
    86941 tcaaaccttg tcaaatgtag acagagtaga gattcttcct tatcatacga tgggaaaata
    87001 caagtgggaa acacttggtt tgaaatatcc attagaaggg atcgaaccac ctacaaaaga
    87061 acgtgtcgaa aatgctaaac gattgcttca tacagaagag tatacaaaat atttaacgcg
    87121 ataggtcgca tcggaaagaa gataagtatt gcttctttcc gtttttttgc tattattaaa
    87181 taggagaaat cacaaaggag cgaatgacat gtcaaaaatt ttagtttttg gtcaccaaaa
    87241 tccagataca gatgcaatcg gggcagccat tgcgttttca cacttgcaaa atgaattagg
    87301 taaagaaaca gaagcagttg ctctaggtac acctaatgaa gaaacacaat acgcattaga
    87361 ccactttcag ctatctgcac cacgagtagt agagtcagct aaagcagaaa atgcagatgt
    87421 catgcttgtg gatcataatg aattccaaca aagtatttca gatatcagcg aagtaaatat
    87481 tttagctgta gtagaccacc atcgtattgc aaactttgaa acagctaatc cattgtacta
    87541 tcgtgcagaa ccagttggat gtacaagtac gattatttta aaactatata aagaaaacca
    87601 agtaacaatt cctaaagaaa tcgcaggaat catgctctca gctatcattt cagatacatt
    87661 gttgttcaaa tcaccaacat gtacgcctga agatgaagca gctgcaaaag aacttgcagc
    87721 aatcgctgaa gtcgatctaa acacttatgg acttgaaatg ttgaaagcag gaacaaatct
    87781 aagtgataaa tcagctgcgg ttcttcttga tttggatgca aaaagtttcc caatgggcag
    87841 ttccaacgtt cgtatcgctc aagtcaatac agtggacctt aatgaagtga tggatcgaca
    87901 agcagagcta gaagcagcaa tggaagatga aatgaaagca aatggctacg atctgttcgt
    87961 attagttgtc acaaacatct tagacagtga ttcagaatta cttgttgctg gtaaaggaaa
    88021 agaaaaagta gaagaagctt tccaaacaac tttgaccaac aaccgtgctt tgcttaaagg
    88081 tgtcgtttca cgtaaaaaac aagtcgtacc tcaattgacg gctgtattta gctaatcaaa
    88141 tggtagtatg aagcattcaa aatgaatgga taagcgattg atataaaaag aaacatagaa
    88201 catactaaag gccgaagaaa tggaggagaa taacagtcaa ttatgactct tgttcttcct
    88261 cctttttacg taaaagcctt ttaagtaaaa taaaacggat agaaaagaga acatatatga
    88321 actattattc aaaaaataag gaaaactttt atcaaaaact gaccgatgat ccgctttttt
    88381 cttcattgac cgattatcta tatgaacatc gagaacaaga aacgattcta agagaattga
    88441 aaaaagaatt ttcgcaaaat aaattttccc attttcttga tttattgatc gatgcaggat
    88501 taatcaagag ggaagaacga agatatcatt tgaactttcc aatatttgat cctaaagatt
    88561 acttgcaaca agccacttcg gctgctgaaa ccatagctga acaattgaag agactctctg
    88621 ttgatgaaca aaaattagcg atgggagaag ttatttgggc gtattgtttc gaagatgaac
    88681 ggaaagaagc gtatttttat ggagtaagaa acagccgaga gacagaattg ctgaggacaa
    88741 ctgcaggaaa tgagaagtac cgttttatca cactttcctc tatagaacac ttcccgctta
    88801 ctttggctaa ttactttttt gtacagaaga atcagcttcc tgtaacgaag gcattcaaag
    88861 aattagccga acttattgga gacgtaaatg aatcgtactt ttttgatcaa atagaagtta
    88921 tcgttgacag gatacgtaag aataaatata aaaatcgccg gcctagtatt ttccatcagt
    88981 ctctacttgt gacgaatact ataaaggaag aagaatcatt cacattagaa ttacctattg
    89041 tcgaaaaaaa caattttgag attgaattac caacattgga tccttcattg acaatggagg
    89101 aaatggcctt tcttaaacga caaatctttt ctgagctgag caagaaattt ataccgcatg
    89161 cattttctta tatcaaagaa tatggaacag ttttagtgag taaaacctaa gagagcagta
    89221 agcaaaaatt atactgactc ttgcaggagg agcctttctc ttcctttatg gttaaaagaa
    89281 ttatttgagt attttgccta tctaccacct attagattca ctacatggtc tggaaacaaa
    89341 ggaatgcaaa aaaatggtga gaaatgtgga atgacaaagg aagtagtgat aagaaaagtt
    89401 agatttttaa ataatcaata ttatgattca gtcaagtatg gcattttatg ggaagaacta
    89461 gctgattaga ttaggaagtc catttgataa tccagaatca ctaagaaacg aatggcgagt
    89521 cattaataat ttatccaatc ttgggttatt cttttagatt gaagtgttta aaactaaaaa
    89581 agcatttatg aagtcgtaat agtcaggctt caaaatgctt tttattttgt ttgttttttc
    89641 tttgccacat aaatacacct agactgacga tgaagagcat atccaacaga tttcctgcga
    89701 cttggatcgt cgaagggttg gtcagtggca ttaaagcaag gacaaaaaag gaaaacaagc
    89761 tgtaaataaa acaggtgaca ggaactaatc gaaagaagag cggacatttc aattggctaa
    89821 aaatgagtga aaaaataaaa taagtaaaaa tggataaaat cacgactaaa ccattcatga
    89881 taaagaagaa aagcgtattg tgattaaacg aataaaaaag aattgctaaa aaagaaaacg
    89941 ttttaaatgc aatcaatacg cttgaagctt cgtttaacgt accaggatgt cgatgaaaaa
    90001 aataggcacc gaaataaaca gaaaacagta agccgcaaag agaagacaaa atcaaaaaaa
    90061 acggattttc ttcggcttct gtgatatcag aatgatccaa aagcaaccag cctaatgcac
    90121 tagaaggcgc atgtgaggtc ttaaggtatg aataacatag aaaaaataag atcgtcaaag
    90181 ttgcaaaaaa agagcctaaa tgtaaaaaat tagtggttct acgttgaatt tccataatat
    90241 ctccaaatta aattgataag tatataaagt ataataacaa attgatgagt atatgtcgat
    90301 gttttttaat caaaataata aataaaaact gtctaacatt taaaatttaa tttagatatt
    90361 agacagtttt taagtttgtg agttattttc cttgtcgttt tctcactttt ttagtccaga
    90421 aaccacctga aatatatttt ggctcataag aaatgataaa agcacgctct tcgatttctt
    90481 tgatcaggcg atacagttca cgttcgcttt ttctaggaga aagtatttcg agaactagcc
    90541 gctcaccttc cagccccatt gcagaagtct gtgtcacacc ataaccgtgt tctctcagta
    90601 tacccggcaa acttttatct tctgcgctat ttgctggcaa aatcgttgtg accatgatgt
    90661 atcctaatgc caatttgtct tcaatcaaaa ttccgacact gatacctact gcgtaaccta
    90721 acgcatagac aaataaattc agtggattat ctaaacgatc taaaacaagg gataagccga
    90781 cgatataaat cgtgatctcg accatactga caagtggagc aatcagtcga tagcctttca
    90841 ttgtcaacat gaaacgtaac gtgttcagag tgatataagc aaaattaatg aggaagatca
    90901 tccccaacat ttttggatca atcatttgta aaactccttt catttatata acaacaatat
    90961 caaaaaaaat cgtaagtgaa gaaaaattga aactttatac atacaaaaaa atgataaaat
    91021 gattataata gaattataaa gataggttag gtgattaaaa tgaaaacggt caatcgtgtg
    91081 ttttgtgcca gccacgtgct aagtctcctt ctcagtatat acttaggtgt ctattcgatt
    91141 caacagccaa ttgaggtaga gaacattgtt tttcagatgt cgctgacaga tggtttaatt
    91201 ttaagcgttt ttttcttttt gatactgttt attggtaata ttttcggtag cgttgccagt
    91261 tttttgaatt tttctattta tccactcctt tcgctagcat taggagtggt gggactttgt
    91321 tctctgtgct tatttcccga gccattcagt cctgcattta tattatttgg tacactgaac
    91381 ctgttccaga caactgttgg agcatggttg ctatggcgat caggaaattt aatgaaaata
    91441 ggtgagtaac aattgaatcc aattatattg aaacgttctg tccatcatat tccagtgtta
    91501 gaagttgttc tgaaagaatt aagagaggaa cgtattcccg ttgtcatcta ttatcacgga
    91561 tggcagacaa gtaaggagtt agttttgacg caaggaagaa aaattgcgca gcagggcatt
    91621 cgtgtgatcc tgccagatgc gatgaatcat ggggagcgca aacagccggt ttcttctatt
    91681 ccatcctata cattttggga tagtatctac ggaaatctgt ttgaatttga cacacttatc
    91741 aagcatttag aaaaaagagg tcttctacat gatcagcttg cagttggcgg ggtttcgatg
    91801 gggggaatga caacatgcgc cttgttggct aaacatcctc atatagtagc gggcgtgtgt
    91861 ttgatgggtt cccctgcacc tttggaatat ggaaaagaaa tcgtaaggcg cgcatcggaa
    91921 tataattatg tcgtaccaaa agactatttt gatttgattt catgggttaa gagctatgat
    91981 ttgtcattgc agccggaaaa gttagctgga cgtcctctgt tcttttggca tggacaagct
    92041 gacgaaaaaa tcccttatat acaaacggaa cgatttgtag aaaaaaatcg gcataagtct
    92101 tacggaaaag aaattgtttt tcgttcctca aaaacacagc gacatatggt acaaatacca
    92161 ctaatggaag aagcttccac ttttttagca aaaaatttga taaaaaagta taaataattg
    92221 aaacggaaaa tcactcatga cgatagagga ttttccgttt tttattttgg gctataataa
    92281 actctgttag gaaaatttgg tataattgat agtaagaatg acaacatgtt agaaaggaag
    92341 agagcatgcc cactttagca gatgttgcaa aacgtgccaa tgtttcaaaa atgacggtct
    92401 ctcgagtcat caatcatccg gaacaggtga ctgacgaatt gaaagagtta gttttctcag
    92461 caatggctga actggattac cgtccaaata ttgctgcaaa agcacttgtc acgaatcggt
    92521 cgcagatcat caaattattt atactggaag aaatcgacac aactgagccc tattacatga
    92581 atttgctgat gggaatcgct aaacggatcg gtaaagcgca ttattctctc caattagtta
    92641 caaatgatgc cttcgacgtc ggttcttgtg atggatacat catcacaggt gtcagaaaaa
    92701 aaagacttcg attggatcga ccgtttggaa aaaccagttg tcttgtttgg ggaaaattct
    92761 gaaggatatg attatgtaga cagcgataat gaatatggta cagcaaaagc aacgcaatat
    92821 gccttagaaa gagattatca aacgattatt tatatcggta tcgaagtgga cgagcctttt
    92881 gaaatttcaa gggaaactgg ttatttatct gtgatgaagg aagttgatct tcctagtaaa
    92941 atcttgcggt ttccaaatca ttcgacagaa gtagaaagat atatgacaga aaactgggca
    93001 gattttacag ataaaacttg ttttgtatgt agttcagatc ggctagcact aggagcagta
    93061 agagggatta taaatgctgg aggacacttg cccgaagaat ttggtgtgat tggatttgat
    93121 ggggtatttt tagaccaaat cagttcacct aaattgacaa cgatcaagca agatattata
    93181 cgtatgggtg aagtatgtgg agaaatgttg ttaaaaaaaa tagaagaaaa aggggcgagt
    93241 caaggttatc gtcgtttttt tcctgaacta gttatcagag agacaacgaa ttaagtctta
    93301 ttactatata tat
//
LOCUS       DAZABD010000002        90289 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000002 DAZABD010000000
VERSION     DAZABD010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 90289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 90289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..90289
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.002"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(192..2126)
                     /locus_tag="ACHALG_000085"
     CDS             complement(192..2126)
                     /locus_tag="ACHALG_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFN2585533.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            2352..3008
                     /locus_tag="ACHALG_000086"
     CDS             2352..3008
                     /locus_tag="ACHALG_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFN2585534.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            complement(3082..3435)
                     /locus_tag="ACHALG_000087"
     CDS             complement(3082..3435)
                     /locus_tag="ACHALG_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFN2585535.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            3627..4556
                     /locus_tag="ACHALG_000088"
     CDS             3627..4556
                     /locus_tag="ACHALG_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFN2585536.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            4567..5403
                     /locus_tag="ACHALG_000089"
     CDS             4567..5403
                     /locus_tag="ACHALG_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HFN2585537.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            5651..6427
                     /locus_tag="ACHALG_000090"
     CDS             5651..6427
                     /locus_tag="ACHALG_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFN2585538.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            6603..7346
                     /locus_tag="ACHALG_000091"
     CDS             6603..7346
                     /locus_tag="ACHALG_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN2585539.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            7339..8919
                     /locus_tag="ACHALG_000092"
     CDS             7339..8919
                     /locus_tag="ACHALG_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFN2585540.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(9170..9649)
                     /locus_tag="ACHALG_000093"
     CDS             complement(9170..9649)
                     /locus_tag="ACHALG_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFN2585541.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(9665..10417)
                     /locus_tag="ACHALG_000094"
     CDS             complement(9665..10417)
                     /locus_tag="ACHALG_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFN2585542.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            10534..10604
                     /locus_tag="ACHALG_000095"
     tRNA            10534..10604
                     /locus_tag="ACHALG_000095"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10566..10568,aa:Gly,seq:ccc)
     gene            10965..11690
                     /locus_tag="ACHALG_000096"
     CDS             10965..11690
                     /locus_tag="ACHALG_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFN2585543.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            11945..12211
                     /locus_tag="ACHALG_000097"
     CDS             11945..12211
                     /locus_tag="ACHALG_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HFN2585544.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            12208..12639
                     /gene="ruvX"
                     /locus_tag="ACHALG_000098"
     CDS             12208..12639
                     /gene="ruvX"
                     /locus_tag="ACHALG_000098"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFN2585545.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            12664..12969
                     /locus_tag="ACHALG_000099"
     CDS             12664..12969
                     /locus_tag="ACHALG_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HFN2585546.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(13050..14195)
                     /locus_tag="ACHALG_000100"
     CDS             complement(13050..14195)
                     /locus_tag="ACHALG_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN2585547.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(14256..14903)
                     /locus_tag="ACHALG_000101"
     CDS             complement(14256..14903)
                     /locus_tag="ACHALG_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN2585548.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            15097..15390
                     /locus_tag="ACHALG_000102"
     CDS             15097..15390
                     /locus_tag="ACHALG_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2585549.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            15434..15745
                     /locus_tag="ACHALG_000103"
     CDS             15434..15745
                     /locus_tag="ACHALG_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2585550.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            15742..16366
                     /locus_tag="ACHALG_000104"
                     /pseudo
     CDS             15742..16366
                     /locus_tag="ACHALG_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            16507..16665
                     /locus_tag="ACHALG_000105"
     CDS             16507..16665
                     /locus_tag="ACHALG_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2585551.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            16666..17028
                     /locus_tag="ACHALG_000106"
     CDS             16666..17028
                     /locus_tag="ACHALG_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2585552.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            17065..17913
                     /locus_tag="ACHALG_000107"
     CDS             17065..17913
                     /locus_tag="ACHALG_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HFN2585553.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            17903..19378
                     /locus_tag="ACHALG_000108"
     CDS             17903..19378
                     /locus_tag="ACHALG_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapE domain-containing protein"
                     /protein_id="HFN2585554.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            19644..20051
                     /locus_tag="ACHALG_000109"
     CDS             19644..20051
                     /locus_tag="ACHALG_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HFN2585555.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            20054..20269
                     /locus_tag="ACHALG_000110"
     CDS             20054..20269
                     /locus_tag="ACHALG_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2585556.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            20273..20653
                     /locus_tag="ACHALG_000111"
     CDS             20273..20653
                     /locus_tag="ACHALG_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFN2585557.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            20787..20942
                     /locus_tag="ACHALG_000112"
     CDS             20787..20942
                     /locus_tag="ACHALG_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HFN2585558.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            21011..21484
                     /locus_tag="ACHALG_000113"
     CDS             21011..21484
                     /locus_tag="ACHALG_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFN2585559.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            21481..23175
                     /locus_tag="ACHALG_000114"
     CDS             21481..23175
                     /locus_tag="ACHALG_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFN2585560.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            23141..23326
                     /locus_tag="ACHALG_000115"
     CDS             23141..23326
                     /locus_tag="ACHALG_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2585561.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            23330..24505
                     /locus_tag="ACHALG_000116"
     CDS             23330..24505
                     /locus_tag="ACHALG_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFN2585562.1"
                     /translation="MAFFKERQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            24498..26021
                     /locus_tag="ACHALG_000117"
     CDS             24498..26021
                     /locus_tag="ACHALG_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFN2585563.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            26077..26361
                     /locus_tag="ACHALG_000118"
     CDS             26077..26361
                     /locus_tag="ACHALG_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFN2585564.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            26348..26683
                     /locus_tag="ACHALG_000119"
     CDS             26348..26683
                     /locus_tag="ACHALG_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFN2585565.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            26978..27604
                     /locus_tag="ACHALG_000120"
     CDS             26978..27604
                     /locus_tag="ACHALG_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2585566.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            28346..29182
                     /locus_tag="ACHALG_000121"
     CDS             28346..29182
                     /locus_tag="ACHALG_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFN2585567.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLVNCYNAFVILQTLGESRLTQVKITETYC
                     SK"
     gene            29247..29681
                     /locus_tag="ACHALG_000122"
     CDS             29247..29681
                     /locus_tag="ACHALG_000122"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN2585568.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(29749..31071)
                     /locus_tag="ACHALG_000123"
     CDS             complement(29749..31071)
                     /locus_tag="ACHALG_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HFN2585569.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(31225..32865)
                     /locus_tag="ACHALG_000124"
     CDS             complement(31225..32865)
                     /locus_tag="ACHALG_000124"
                     /EC_number="4.1.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043883 - malolactic enzyme activity
                     [Evidence IEA];
                     GO_process: GO:0043464 - malolactic fermentation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malolactic enzyme"
                     /protein_id="HFN2585570.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            33195..33665
                     /locus_tag="ACHALG_000125"
     CDS             33195..33665
                     /locus_tag="ACHALG_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HFN2585571.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            34048..35151
                     /locus_tag="ACHALG_000126"
     CDS             34048..35151
                     /locus_tag="ACHALG_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HFN2585572.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            35435..36115
                     /locus_tag="ACHALG_000127"
     CDS             35435..36115
                     /locus_tag="ACHALG_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFN2585573.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            36205..37239
                     /locus_tag="ACHALG_000128"
     CDS             36205..37239
                     /locus_tag="ACHALG_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFN2585574.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            37253..37588
                     /locus_tag="ACHALG_000129"
     CDS             37253..37588
                     /locus_tag="ACHALG_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2585575.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            37572..40052
                     /locus_tag="ACHALG_000130"
     CDS             37572..40052
                     /locus_tag="ACHALG_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2585576.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            40108..41667
                     /locus_tag="ACHALG_000131"
     CDS             40108..41667
                     /locus_tag="ACHALG_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN2585577.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            41664..43028
                     /locus_tag="ACHALG_000132"
     CDS             41664..43028
                     /locus_tag="ACHALG_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFN2585578.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            43199..44269
                     /locus_tag="ACHALG_000133"
     CDS             43199..44269
                     /locus_tag="ACHALG_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN2585579.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            44284..44952
                     /locus_tag="ACHALG_000134"
     CDS             44284..44952
                     /locus_tag="ACHALG_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN2585580.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            44972..45550
                     /locus_tag="ACHALG_000135"
     CDS             44972..45550
                     /locus_tag="ACHALG_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN2585581.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            45953..46864
                     /locus_tag="ACHALG_000136"
     CDS             45953..46864
                     /locus_tag="ACHALG_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFN2585582.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(47120..48037)
                     /gene="rnhC"
                     /locus_tag="ACHALG_000137"
     CDS             complement(47120..48037)
                     /gene="rnhC"
                     /locus_tag="ACHALG_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HFN2585583.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            48239..48676
                     /gene="zapA"
                     /locus_tag="ACHALG_000138"
     CDS             48239..48676
                     /gene="zapA"
                     /locus_tag="ACHALG_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFN2585584.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            48734..49282
                     /locus_tag="ACHALG_000139"
     CDS             48734..49282
                     /locus_tag="ACHALG_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HFN2585585.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            49397..51757
                     /locus_tag="ACHALG_000140"
     CDS             49397..51757
                     /locus_tag="ACHALG_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFN2585586.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            51844..52158
                     /gene="trxA"
                     /locus_tag="ACHALG_000141"
     CDS             51844..52158
                     /gene="trxA"
                     /locus_tag="ACHALG_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFN2585587.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            52340..54118
                     /gene="uvrC"
                     /locus_tag="ACHALG_000142"
     CDS             52340..54118
                     /gene="uvrC"
                     /locus_tag="ACHALG_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFN2585588.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            54263..55162
                     /locus_tag="ACHALG_000143"
     CDS             54263..55162
                     /locus_tag="ACHALG_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN2585589.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            55155..56417
                     /locus_tag="ACHALG_000144"
     CDS             55155..56417
                     /locus_tag="ACHALG_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN2585590.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            56580..58478
                     /locus_tag="ACHALG_000145"
     CDS             56580..58478
                     /locus_tag="ACHALG_000145"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HFN2585591.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(58777..59496)
                     /locus_tag="ACHALG_000146"
     CDS             complement(58777..59496)
                     /locus_tag="ACHALG_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFN2585592.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     misc_feature    59686..59733
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF03071"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="sodium ion sensor (DUF1646 type); this
                     cis-regulatory element may regulate processes involved in
                     with the transportation of sodium ions; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF03071"
     gene            59833..60165
                     /locus_tag="ACHALG_000147"
     CDS             59833..60165
                     /locus_tag="ACHALG_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2585593.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            60152..62155
                     /locus_tag="ACHALG_000148"
     CDS             60152..62155
                     /locus_tag="ACHALG_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HFN2585594.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            62174..62644
                     /locus_tag="ACHALG_000149"
     CDS             62174..62644
                     /locus_tag="ACHALG_000149"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HFN2585595.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            62665..63249
                     /locus_tag="ACHALG_000150"
     CDS             62665..63249
                     /locus_tag="ACHALG_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HFN2585596.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            63264..64250
                     /locus_tag="ACHALG_000151"
     CDS             63264..64250
                     /locus_tag="ACHALG_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HFN2585597.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            64240..64551
                     /locus_tag="ACHALG_000152"
     CDS             64240..64551
                     /locus_tag="ACHALG_000152"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HFN2585598.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            64573..66354
                     /locus_tag="ACHALG_000153"
     CDS             64573..66354
                     /locus_tag="ACHALG_000153"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HFN2585599.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            66347..67723
                     /locus_tag="ACHALG_000154"
     CDS             66347..67723
                     /locus_tag="ACHALG_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HFN2585600.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            67730..68365
                     /locus_tag="ACHALG_000155"
     CDS             67730..68365
                     /locus_tag="ACHALG_000155"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HFN2585601.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            68395..69750
                     /locus_tag="ACHALG_000156"
     CDS             68395..69750
                     /locus_tag="ACHALG_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFN2585602.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(69942..71336)
                     /locus_tag="ACHALG_000157"
     CDS             complement(69942..71336)
                     /locus_tag="ACHALG_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFN2585603.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            71428..72399
                     /locus_tag="ACHALG_000158"
     CDS             71428..72399
                     /locus_tag="ACHALG_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFN2585604.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            72396..73178
                     /locus_tag="ACHALG_000159"
     CDS             72396..73178
                     /locus_tag="ACHALG_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HFN2585605.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(73347..73718)
                     /locus_tag="ACHALG_000160"
     CDS             complement(73347..73718)
                     /locus_tag="ACHALG_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFN2585606.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            73852..74295
                     /locus_tag="ACHALG_000161"
     CDS             73852..74295
                     /locus_tag="ACHALG_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFN2585607.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(74338..75147)
                     /gene="yidA"
                     /locus_tag="ACHALG_000162"
     CDS             complement(74338..75147)
                     /gene="yidA"
                     /locus_tag="ACHALG_000162"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFN2585608.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(75166..76533)
                     /locus_tag="ACHALG_000163"
     CDS             complement(75166..76533)
                     /locus_tag="ACHALG_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFN2585609.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(76517..77347)
                     /locus_tag="ACHALG_000164"
     CDS             complement(76517..77347)
                     /locus_tag="ACHALG_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HFN2585610.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            77510..77947
                     /locus_tag="ACHALG_000165"
     CDS             77510..77947
                     /locus_tag="ACHALG_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HFN2585611.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            78023..78643
                     /gene="rpoE"
                     /locus_tag="ACHALG_000166"
     CDS             78023..78643
                     /gene="rpoE"
                     /locus_tag="ACHALG_000166"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HFN2585612.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            78832..79338
                     /locus_tag="ACHALG_000167"
     CDS             78832..79338
                     /locus_tag="ACHALG_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFN2585613.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            79355..80254
                     /locus_tag="ACHALG_000168"
     CDS             79355..80254
                     /locus_tag="ACHALG_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HFN2585614.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            80341..81225
                     /locus_tag="ACHALG_000169"
     CDS             80341..81225
                     /locus_tag="ACHALG_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFN2585615.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNYTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(81417..81488)
                     /locus_tag="ACHALG_000170"
     tRNA            complement(81417..81488)
                     /locus_tag="ACHALG_000170"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(81453..81455),aa:Arg,seq:ccg)
     gene            81548..82882
                     /gene="rpoN"
                     /locus_tag="ACHALG_000171"
     CDS             81548..82882
                     /gene="rpoN"
                     /locus_tag="ACHALG_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFN2585616.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            83031..84122
                     /locus_tag="ACHALG_000172"
     CDS             83031..84122
                     /locus_tag="ACHALG_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HFN2585617.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            84163..85164
                     /gene="gap"
                     /locus_tag="ACHALG_000173"
     CDS             84163..85164
                     /gene="gap"
                     /locus_tag="ACHALG_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFN2585618.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            85274..86464
                     /gene="pgk"
                     /locus_tag="ACHALG_000174"
     CDS             85274..86464
                     /gene="pgk"
                     /locus_tag="ACHALG_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFN2585619.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            86568..87323
                     /gene="tpiA"
                     /locus_tag="ACHALG_000175"
     CDS             86568..87323
                     /gene="tpiA"
                     /locus_tag="ACHALG_000175"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFN2585620.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            87439..88737
                     /gene="eno"
                     /locus_tag="ACHALG_000176"
     CDS             87439..88737
                     /gene="eno"
                     /locus_tag="ACHALG_000176"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFN2585621.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            89136..90275
                     /locus_tag="ACHALG_000177"
     CDS             89136..90275
                     /locus_tag="ACHALG_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HFN2585622.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT        29922 a        15554 c        18748 g        26065 t
ORIGIN      
        1 taataagccc aaaaataatg gggtaaatac accttgtatt tggggtgatt actattattt
       61 agaagcttta gtgagaatga ctagtaattg gtcttcttat tggtgaaaaa ggtaaatatg
      121 gaatttttgt ttgataaaat aaaaaaagaa aaaccaaaac agcatgaatg gtttctgttt
      181 tggttttttc gttaattttc atattcttct aattccaagc ttagttcttc ccattccgtc
      241 aattttgttt cttgatcttg tctagcagct tctaaatctt ggttcagctc attgagtttt
      301 acgtgatctt ccaggttttc tggcgcgacc atttcttgtt ccaactgatc gatccattca
      361 tcgatccgcg ccatttcttc ttcaacctga gtaatttttc gttgaaggct acggataagc
      421 ttttgctgtt ctttgctttg gtaataatca gatttgttcg tgggagcagg agcagattct
      481 ggctcatctt gtgccaacaa ttcggcgatt tcttcttctt cctgcttttt ctctaaataa
      541 taatcatagt ctcctagata gactttgctg ccattttcag agagttcgac gacttttgtc
      601 gcgatccgat tgatgaaata tcggtcatgg gaaacaaaaa gcagtgtgcc gtcataatcg
      661 atcaatgcat tttctaatac ttctttgttg tcaatatcta aatggtttgt tggttcatcc
      721 aaaatcaaaa aattttcttt gttcatcgct aacttggcta aagcaacccg tgctttttca
      781 ccgccactta gcagagggat tggtttttct acatcttcgc cagaaaataa gaaagcacct
      841 aaagcacttc ggatctcttt ttctggagtg gtaggatgct catcccacaa ttctgctagg
      901 atcgtcttgt ttgtatgcag atcagcttga ccttgatcgt aatagccgat ggtgacattt
      961 gtccctagcg tttcttttcc acggataaaa gggatctggt gaatgattga tttcagcaac
     1021 gtcgatttcc cgataccatt cggacctact agagcaatcg cttcttgacg acggatatct
     1081 agattgattg gttcagataa ggtgacatta tcatatccga tagcggcatc ttcgatttgc
     1141 aagactacat ttccagaagg cttttctata ctgaatagaa agtttgcttg tttttcatcc
     1201 ccttgaggac gatccagccg gtccattttt tctaatgtct ttctgcggct ttgcgctcgt
     1261 tttgtggtag aggcgcgaac aaggttccga gcaacgaagt cttctaattt atggatttct
     1321 gtttgctgct tttcataagc cttccattca cttgctaact gttctgcttt caaatctaga
     1381 tatttcgaat agttaccttt gtaatggcgc atttttcttc tgcttaattc atagacttca
     1441 ttgactactt tatctaaaaa ataacggtca tgggaaacaa tcaataatgc accagtatag
     1501 ctttgcagat aattctcaag ccaggaaagt gtctcgatat ctaaatggtt cgttggttca
     1561 tctagaatca aaatgtcagg gtgttgcaaa agcatgcgag ctaaagctaa acgtgtgcgc
     1621 tgtccgcctg ataaagtatc gattttctga tcataaaatg aagaatcgaa tttaaaccca
     1681 tgcaatacgg aacgaatttc attttcataa ccatagccgt ttcgatctga aaaatcgtgt
     1741 tgcagccggt cgtattcttt taaataagaa ctataagcag gtgtgtctgg cgtaccttcg
     1801 ctaatgaaaa tctctagctc gcgcatccgc tcttccattt gtctaacttg ttcaaaagct
     1861 ttcagcatct cgttccaaac tgtttcattg gaatcaagtc ctgtgtcctg agcgaggtat
     1921 ccgagagtcg ctgttttatt tttagcgatc tttccttcat caggtgcttc aattccagca
     1981 atgatcttta ataaagtcga ttttccagcg ccatttctgc caactaaagc gacacgagcg
     2041 cctgtactga tttctaaatg tatgttttca aataagactt ctgcaccaaa atggcgagca
     2101 atctgattgg cttgtaataa tatcatggaa tcctcttttc tactttgtga attctcctca
     2161 aataaaaaac tatttttcaa ttagtagtct accataaatt ccattatact gcgagatttg
     2221 cagaaatgat aaataaatga ataaagaata aaatttcaca atatctgagc aatggaattg
     2281 attttttcct ataaatactg ctatgatgga gaaatcaaag atgtaattag acaatacgtg
     2341 gaggtcattt cgtgaaagat catattattc caaaggcgac agctaaaaga ctgcctttgt
     2401 attatcgcta tttaaggatt ttaaataatg ctggaaagaa aaaagtgtct tcaactgaat
     2461 taagtgaagc cgttcaagta gatagtgcaa cgatccgtcg agatttttct tattttggcg
     2521 aattaggaaa acgtgggtat ggttatgatg tagaagatct tatgcacttt tttgcaaaga
     2581 cattaaacga cgatcaattg acaaatgttg cgctgatcgg tgtagggaac ttgggaagtg
     2641 ctttactaaa atacaaattc catcaaagta atagcatacg tatcagttgt gcttttgatg
     2701 tgaatgagga aatcgttggg cgcatcgtag acggtatccc cgtttatcca atggaagata
     2761 tgtcagaaca aatcaagatc caacaaatcg aagtagcaat tttaacgata cccgctgaaa
     2821 gagcacaaga agtagtcaat cagttaacgg aagcaggaat caaaggaatc ttgaacttta
     2881 ccgctgctcg attgaatgct ccgtcagatg tattgatcca aaatgttgat ttgacgaatg
     2941 aattgcaaac cttgatctat ttcttgcatt ctgacgcaaa tgcgcattcc gaaaatacag
     3001 cagaataaaa ataaaaagcg attgaacaaa tgaaggcaaa actttcactt gttcaatcgc
     3061 ttttttctgt ttggacaacg attattttgt tcggaagatt tcttcataag aaagggaatc
     3121 atcttctgct gtcagttcat ctagtagata acggattttt ttaaagtcga tgaaccgtgc
     3181 ttgccgaaag acttcttttc cttgatgaaa aatcaggaca actggcgcag tcagcacttc
     3241 aaatttacta gctacttctg gtgtttcaaa tgcatccaaa tgaagtgccg gaatatcata
     3301 atgtgtgaga agttcctcta gtcgtggacg aacagcgtga caaacactgc aattaggcat
     3361 cgaaagataa atgacggcaa cggatgactg ttgcagtatg tcttgaactt cagtcaagtt
     3421 agtggtttgt ttcattttta ttctcccttt tatgatagaa gtgatatgta tgagacctta
     3481 atttaatggg aataacagaa aaagtcaatt gaagtgcttt ggtaaaaaat aagagttgtg
     3541 taaaaattta cagagcaact tgtttttttt gaataaaagc gtatatttgc aaagcgaatg
     3601 aactagaaat aaaaaggagg aaactcatgt tttcttttat tccaaattcg gttttgcaga
     3661 tgagtacgat tttcttatcg atcgttattg aagcattacc tttcgtatta ttaggctgtc
     3721 ttatctcagg cgccttacaa gtatttttga ccccagaacg tgtgaagaga tggcttcctg
     3781 aaaatcgtat cttctctatt tttactggtt cgatcttagg atttttcttt ccttcatgtg
     3841 aatgcggcat cgtaccgatc gttcatcaat ttgttaaaaa aggcgtacca gttcacacag
     3901 cgtttgcttt tatgctgact gctccgatca tcaatccaat cgtgattttt tccactttta
     3961 tcgcatttgg caattcttgg cagatggcaa gctggcgggt gataggcagc ttttttgttg
     4021 cccttgtgat tggtgtctgg cttgcctatt ttcaaaaaga cgctgtattg aaagaaaaaa
     4081 tcacgtctaa agtagcagaa aatcaccatc atcatgtcca tgaagaagaa caaagtgaga
     4141 agaatgaacc aaaaaatcag ctgatccatc aaactgggca tgtgttgaca cattcaatcg
     4201 acgaattttt tgatacaggt cgttatttga tcattggcgg attaatcgca gctgcgatgc
     4261 agacttattt accaactaga attatgctaa cgctcggctc aacaaagatt ttagccatta
     4321 tcatcatgct gttattggcc tttacgatgt ctctttgctc agaagcagat gcgtttatag
     4381 gatcttcttt gttaagtctg ttcggcacag cacctgtagc tgcctttttg gtctttggcc
     4441 caatggtcga tatcaaaaat ctactgatga tgaaacgata ttttacagga cgctttattt
     4501 tatcgctgat cggactgatt gcccttagcg ttattggatt cacattattt atttaggagg
     4561 gaaaatatgc tgcggttttt gattttatct gggtatttcg aattgatgat gtacttgcaa
     4621 gtttctggca aactcgatca atatatcaat gtccattatc gctacttagc tattctttca
     4681 atgattttat ctgctctttt ggctttagtt cagctgtatt tgtgggtcaa aaacggaaaa
     4741 gcagaagaaa aggcacacga gcacgatcat gaccatgggt tgtcaaaacc ttatcaacgt
     4801 ggaatcgctt atgtactgct agctttgcca gtaatcgtgg ggacgttgtt tccaacagtt
     4861 agtctcgaca ctacgatcgt agaagccaaa ggatttaatt tcccagtaag taaagaatca
     4921 gtaggggatc cggagatgca gacacaatac ttaaagccgg ataccagtat ttatttcaac
     4981 aaatcagact acttagacca aatgaatgag ttgaaagaaa agtatgggga taagcaaact
     5041 atcaagatca cagatgaaaa ctatttagaa gttatggagc taatttataa ttaccctagc
     5101 gaattcattg gcaaaaaaat cagctatgaa ggatttgttt accaaacacc gaacgatagc
     5161 aatacagata tcttcttgtt ccgattcggc attatccatt gtgtggcaga ttcaggagtc
     5221 tttggtttgt tgacgcatat gccggaagga gtaacggtta aaaatgatga atggtataaa
     5281 atagaaggaa ccatccaatc agactattat caaccattca agcgtgaaat cccatctgta
     5341 gttgttacaa acgctgagaa agtctctgct ccgaaaaatc agtatgtgta tcgttctttt
     5401 taaaagaaca aaataaataa acatagctca gacagctgac acgcaaggac gtcggttgtc
     5461 tggctttttt tgcatgatgt attcatttgt ttgatattta ttttagaagc agcatcctga
     5521 aagtatttga tatcggtttc aaaggtgttg gaaaagattg tgaagagaat tacaatttat
     5581 tcctaacatg tatgttgaaa agttgtatga aatgattttg atagtaaaat atcgagtgga
     5641 ggtacttttt atggtaacgc gtatatttat tttggatgat agtttggttt ttgaaaaaat
     5701 gctagtagaa attttggaag aatccagaat tttattaagt ccttggaaat ttgagatcgt
     5761 tcagggattg aacgttatac aatttgttga attcattaaa aataatgaaa tctattcctc
     5821 agatattttt ttcctagaca tcgatttgaa cgcctattat agcggtatcg aactaggcga
     5881 aatgcttaga gaaaagaatg aaaagtgtaa aattgtttat ctaacgtctc atccgaataa
     5941 agcaataact gttattaatc gaaagattac gcctagtggt tatttgataa aagaattcac
     6001 ttatgaaaaa aataaaaaaa gcatccaaag ttacttattg aaagaaaaag aaaactatcc
     6061 tgttcaacat gctacgaaaa aaaggtggac aactgtccgt tatggtaata gcgatcacta
     6121 tatccattac gatgaaatcc tttatctaat gagcatgcca ggttataaaa ataaaatagg
     6181 cctagtattg aaagatgaag agattctcgt aaacggcaca attaaaaaat ataaaaaatc
     6241 actcgaatat gagtatttat gtaaagattt aaaagcttat atcattaata ctgaaaacat
     6301 tacatctatt agcagagtaa acgaaatagt aacatttgca aatggagaac aactagagat
     6361 gggaaagaga agtattgata aactaaagaa ttttatgcaa gaaaagatag aaaatgaatc
     6421 attttaaaga aaacaaagac tcatatctta cctgttagaa gagtgttttc ctgtagaaga
     6481 gaacaaccca agtagcagcc tagtcgtttc tatccttttt tgttatgcac tctttttgac
     6541 atttactcca ccaataacga agggtacaat aaaaagtaat aacaaacaaa aggagtacag
     6601 aaatgattga attcatcaat gtaagtaaaa gttacgggaa aaaacaagca ttaaaagact
     6661 tgtctctctc gataagacaa ggggaaattt ttggattttt aggacataat ggtgccggaa
     6721 aatcaactac gattaaaagt ttagttagta ttattgaacc atcatcggga acgattttag
     6781 tagatggaat gaaattgacg gaaaatcgat tgagtatcaa acaaaaaatc ggttatgtgc
     6841 ctgattcccc agatattttt ttacaattga cggctggaga atattgggac ttgatttcag
     6901 ctgcctatga attagacaca caaaaaaaag aaaagcgttt agcagaattg acagcgcttt
     6961 ttgatatgta tagtcatcaa aatgaaacaa tcgcaagttt ttcacatgga atgcgtcaaa
     7021 aaacgatttt gatcggcacg ttgctgccag atccagatat ttgggtattg gatgaacctt
     7081 tgcaaggact tgatcctcaa gcagcatttg atttgaaaga aatgatgaag gctcatgccg
     7141 caaaaggaaa aacagtcatt ttttctacac atgtattgga taccgcacaa cagttatgcg
     7201 ataaactggc tattttgaag aaaggtgaat tgatttatca gggggcagtt agtgaattat
     7261 tgaatggctc acctgatgaa acgctagaaa agatttattt gaaaatggct ggaaggcaag
     7321 cgacaggaga tggaccgtat gtataagcga caattattag aattaattcg agttaatctt
     7381 ttatatgtta atccacagct cacgaataaa gcaagagaga aaggaaaaaa gggaaagcga
     7441 ttaactatct atcttttgtc tcaaaatctt ttttcagggc tgattttttt actgatttac
     7501 ggatttgcta tgtttgctat gaactttgtt aaattgccag gtttctttac ttattacgtg
     7561 gcactttttg gtatgttagg tttaactcaa agtatatcag tcatttataa tatctttttt
     7621 gaaggaaatg acctgcaagt atttttgcct ctgccttttg gacaagaaca ggttttttca
     7681 gcaaagattt tggtagttgc tttgaccatc attccttttg tttttccgct tctagtcatg
     7741 tttattttaa ccggatggcg agctgatcta gggattttgc ttactatcat tatctcaatc
     7801 cttttgtttt ctctattttt gattattctt ttttgtctgg ctagtttgat tgtttttggt
     7861 ttggctcgaa cagcagtgtt taaaaaacat agaaaaacag tttctagtat cttgcttggc
     7921 agctcgatgc tgatcgcatt tgctggaatc atgtggatga atcatcaaac ggggactatt
     7981 gaaaatgagt taacagatcg gcatccgatt tcgatattat tgccattcta tgatgctgcc
     8041 agtcagccac tttctcaaca aggtttattg ggttggggaa tactagctat cacggctctg
     8101 ggatttttcc ttgctgtaaa acagctgata gtaccaaagc ttacagagca actgactgat
     8161 tcaaacagtg agttcaaaat aaaaagaacc cataaaaaga accaaaatgt gcatcagctt
     8221 ttatttagtt acaatgctca attagtaaaa gaacctaatt tgattatgca agttctttct
     8281 tcttccttgt taactccaat catttttatt cttgcttttg ccctaggagg cgaaataaaa
     8341 ttaactgatt tagataataa gttaatcggt gttgttttcc tggtagggat cgcattggct
     8401 ttcttaacag ttaatcagac ttcttttatc agcaatctta tttctttgga tcaagaaaat
     8461 tttctgttta tccgttcttt gccaatctct atgaaccaat atttaaaaga aaagttcaga
     8521 ttcggatgga tattgcagag ttttttaact ggtgggatcg cattattaag cggactgtct
     8581 tttcagctgc ctttgttttt tatccctagc ttgctatttg gttctttatt cggctgctac
     8641 ttattgtcac tacgttattt cgctagagat tatcggttgc tgctgctgaa ctggaccaat
     8701 attaaccaat tatttaacag aggttcagga agtttgggtt tagtcgcaac tatgatgggt
     8761 tcattgatca tcagtattat tcttatcgtc ttatacggaa tgttggcttt gttttatcca
     8821 ttttggttag tgaatattcc tgtgatcggt ttagttctaa tactttccgc tctatggatc
     8881 gtttattatc aacgaacgtt ttggaaaaaa tttgaataaa gaagaggggc cgtgacaaaa
     8941 atcatgtcgc agcccctttt ttacagatag agggtgttca aaagtcagtt tctcagtatt
     9001 aagtcaattt tatgtaaaaa tgtgagaagc tatttttaca aaactttttt taatactcat
     9061 aattacttct tattgctcct gcggttactt gtcgcaatgt ttgtgagata ttttgtcatc
     9121 gctcaaggct gaacgtctct ttcacaacct tttctttgtt acacttcgtt tatttaaaaa
     9181 gccgtttttt tcgtttcatc atgattgcgt cacgctttac tttgaaatta cggatgttat
     9241 ggttaaagac ttctgataat acaagaccta aagcgattgc tccagcaatc ataattgctt
     9301 ggaccgcata atgagccgct tctcgatagg attcagtaac gagatttcga accgcttgat
     9361 aagctaaacc accagggact agaggaacca tcccgggaat attgaaaatc gttacgggca
     9421 ttttcaaacg tttagagaaa agatcgctga tgaatgctac tcctaaagaa ccaagcaatg
     9481 aaccaaaagc agcagatccg cctatttgaa cacagatcca gtagatcatc cagccaaaag
     9541 caccagaaag accacaacaa atcaatgaac gcctaggtac atttgtaata acggcaaatg
     9601 ccgcagtcgc taaaaaacta aaagaaaact gaataaatat cgtccccata cttgtgttcc
     9661 tttcctaata aaaaagctgg aaaataaaag caatcgcaaa accgatcatc gtagcagtca
     9721 tcatcgcttc tgttccgcga gaaactcctg agacataatg tccagctagc aaatcacgga
     9781 tagcgttcgt aatttgaact ccaggaacta aaggcatcac acatccgata atgattagat
     9841 cctgattgct tcctaagcca aaacgtacgc tgtagatagc ggctatgcca atcaataaag
     9901 aagataaaaa ttccgataaa aatttgattc taaaaaattt caaactatat agataagctg
     9961 cgtatcccaa tcctccaatg actaaagtca caggtaaatc ggtcatcttt ccgccaaata
    10021 acaccataat tgttccactg atcactgccg cgctaacgat ttgcaaccag acaggaaaga
    10081 aagaacggtc tcggtcgatt gcttgcagtt cgcagtaaag ttcttctaat gttagctctg
    10141 aagcaacata tttacgtgaa agatcattta cttttactac tttttctaaa ttaatcgaac
    10201 ggttggtaat ttgttccatt cggatggtcg aggtacgatc aagaccaata aagagaccgg
    10261 tttgagtgac atagctgacc aaacggaaat ttccagaagc taaagcaatt cgactcatcg
    10321 tatcttctac ccgatacatc tccgcattgc tttccatcat gattttccca gcgagtaaac
    10381 aagtgtccag caacagatct aattcttttc ccgtcatagc atcttctcca ataattgatt
    10441 gattagtttt attttactac aaaacatacc agaagcgtaa agaataaaaa aacttgagga
    10501 aggaataaaa gaggcgtata ctgtttttgt attgcgggtg tagtttagtg gtaaaatccc
    10561 agcttcccaa gctgatgtcg cgggttcgat tcccgtcacc cgcttaaaat aggttgtgaa
    10621 aaagctgctc tgcaacaagt aataagaaca gccaagcaaa aacaacttct tatgttttta
    10681 acttggatgg acttattacc gcagatgcaa gcttttgcgc accgtttacc aagcggttgt
    10741 gaaatcagtg tattgtcttg agtatgaaga aaatttttga ataataataa ataccttaca
    10801 gatagagtgg tgactgatta gagacgagga atccgtctct tttttgtatt tttaagccat
    10861 cttaaaacag gttttatttt ttgctgtttc tatttctttc tagtatttat ttatagggga
    10921 acatatgtta agatttaggg agctgttagt aaaggagtaa agaaatgagc aaaaaaacaa
    10981 ccaaggcgat cgtggggatc gtcgctgctg tgattgcagg ggcattcggt gtttccacca
    11041 cacaaaaaga ttcagatatc tttcagcaga ttacaactat ttttgattcc acaccacaag
    11101 cttctcaagt agcggatgtg ccagaatatg atggaaaaca tcaagaaata gaaatcaatg
    11161 ggaatcagcc ggaatttaca aaagaggatc ttagcctttc caaaggaaca tgggaaagct
    11221 attcaaatat tgataaattg aacagagtag gacaagccaa tgctatgctt ggaaaagatt
    11281 tgttccctac tgaaaagaga gagcctttgt atattgaccc gacaggttgg aaacaaaaga
    11341 aattaagtga cgggcaatgg ttatataatc gcagccattt aattggtttc cagctgactg
    11401 gtcaaaataa caacattaaa aacttgatga cagggacacg ttcgttgaat gctccataca
    11461 tgcttgctca cgaaaatgat attgcggcat atatcaaaga aacaaatcat catgtacgct
    11521 accgtgtcac tcctcatttt gaggggaatg aactggtagc caggggagtg caattggaag
    11581 cagaaagcat tgaagacaaa aaaattgaat tcaatgtctt tatctacaat gttcaagatg
    11641 gctacacgat caattatgag acaggacaag caacaaaaac aaaaaattag tagcagctat
    11701 tcgaattttt tatgaatatt atgaaaaaat tgtgtaaaaa catacattca aagaaatata
    11761 gcgtatactt tatgaaaaga gtacttttga taaagctaca gaaaaaagga aaaaaacata
    11821 caaatagtgt ttcttaattt attcggaagt ttggaatatt tgtttgtagt tttccctttc
    11881 ttcgagtaaa ataaggttgt agaaggtaag ctggaattac cttgcgggaa agaggtgtag
    11941 aatcatgggt tttacagatg aaaccgtacg ttttggtttt gacgatagtc gcaaaaaaga
    12001 agtgagcgaa acattagcga ttgtttatcg ggctttggaa gaaaaaggct acaatccaat
    12061 caatcagatt gtcggctact tgctttctgg agacccggcc tacattcctc gttatcgtga
    12121 tgctcgaaat ttgattcgcc gtcacgaacg cgatgagatc atggaagtaa ttgtcaagga
    12181 ctacctctca aatcatggag tgaatttatg agagtaatgg gcttggatgt cggttctaag
    12241 acagtaggtg ttgctatcag tgaccccttt ggctggacag ctcaaggagt agagatcatt
    12301 cggatcaatg aagcagaaga agagtttggc tttgatcggt tgaaggaatt agtagcacaa
    12361 tatgaagtag accggtttgt agttggatta cctaaaaata tgaacaattc catcggacca
    12421 agggcagaag cttccatgtc atacggagaa aagatcaaag aactattctc cttgccagtt
    12481 gattatcaag atgaacgatt aaccactgtt caggcagaac gtatgctagt agagcaggca
    12541 aatacttcaa gagccaaacg aaaaaaagtc atcgataaat tagcagctgt gatgatctta
    12601 caaaattatt tagacggctc aggtaagcaa ttattttaat aataaaagaa aagaggtaat
    12661 cttatgacag aacataacca ttcccacgac catgaaggac acgaacacat cacattggtg
    12721 gatgaccaag gaaatgaaac attatatgaa atcctactaa cagttgacgg acaagaagaa
    12781 tttggacgta actatgttct tttatatcct gctggtgttt cagaagatga agatgtagaa
    12841 ttacaagctt atgcatacat cgaaaacgaa gatggaacag aaggcgagtt agaacaaatc
    12901 gaaacagacg cagaatggga catgattgaa gaagtattca atacattcat ggcggaagaa
    12961 gaagaataga ctataaagct aggagaaacc ttgatatgat agggtttctc ctttttttat
    13021 tttttactgt atgccatatt gtatgccatc tagtcaatat ttaagtgttg tactaatttt
    13081 tcaacagtct tttctttcgt ttcttttgcc aagtgaccgt aacaatcgat cgtctgacta
    13141 atttttgtat gccgtaatct agtttgaact tctaccatag tagctccagc gtccaagagc
    13201 attgaagcat gagtgtgtct tgctacatgc acaccgagaa atggaaccat agctttttca
    13261 caaatagtaa tcaatcttgc ataaatagcg tggtgtgtga ttagttcttt atagatacca
    13321 caaaaaatca tagtcggatc agttacacca agtgtcagca tatatttccg ctgattaatc
    13381 tgccattttt ttaatataga aattgttctt tcatctagtg gaagaacccc agcactacaa
    13441 tcagttttag gatcactgat tttatagcca tttttggatt ggcttagtgt tttattgatg
    13501 gtgaccgtat tttcttgaaa atcaatgtct gaccaattca atgcaagggc ttcactgatc
    13561 ctaaccccgc tataaaagag aaagcgtagt aacgttttat caaagtcgtt tcgataagtt
    13621 tcaggtttgc tgtttaggta atcaaacaag gtgatcagtt gttctttact gtaaactttg
    13681 accttttctt tatgcttagt ggtggttctt ttaggcatta atacgttgtc tagtggatta
    13741 gtctcaataa tcccaagatt gatagcgtag ctaaaaatac gcttagaagt tgaatgaagt
    13801 accttgtatt ggtcagtagt ttcagcccag ctatttacaa tttgttgggc tttttttacc
    13861 gtgacttcat tcacacgaag atttccatat tttggaagaa tccactttct aaacttgatt
    13921 tcagttttca tgaaggtggc ttctcggaca gtggttttat atgtttctag ccacaagtca
    13981 taaacttctt taaacgtttc gttgttcgct tttttaatac cgtttttatc gtagtccact
    14041 tgcaagcgtg acaaggcaag ctgggcttct ttttttgttt tgtaacctcg tctacgaacc
    14101 ttatcaggtt tgccagtcat tttgttcatt ccaagatagg cttcatagag ccaaaattta
    14161 ccttttttag tttcatattg cttgaatgtt gccataatag ttttccctcc atagtgtagc
    14221 aagccgtggg ggcggttgga ggaggcagtt tattattatt ttgaatagtg tttatttagc
    14281 tcagtaacaa gagctgtgaa ctcttttaga agatcatttt cataaaattc ttgatactct
    14341 tttttctcat ctgtattttt ttctgaatca ttaattaatg agtcatatcg taaaagcgta
    14401 tttagaagag ctgcgagata agcaattggg gtactcccgt caggaaattc aattcgagat
    14461 cctgttttaa gatattttat tgcgtttgtt aaataaatct tctgtgagac agtcaggtta
    14521 tcttcttcga tgttattgat agcattagaa agattagaat caattataga gttatttttt
    14581 tcaatagttt ttttcaattg cttttcaagc acatttcgct taatttcggg catcatatgc
    14641 atatctctta atgtataaga tccgtttaat agataggtag tggatacatc taataccttt
    14701 gaaagttgtt taagcctggt gttattggga agattatagc cattttccca attactaacg
    14761 gcacctttac tggccggtgg ggataattta cctccaagtt gttccattga gagtcccttt
    14821 tcaattctag ccagtctaat tctttgtcct gtctccactg cttcggggta tttttttcta
    14881 gccacatttt caccaccatt catatatttt ctctactata tcacgattta aatatgtgtg
    14941 aaacataaaa gttcaaaaaa gtatgttaaa attcttgcaa atgaaaaggt atgatggtat
    15001 attgagttta taagaggtat agaaatgtat tcaagagcct cttgcatttt tttaaaatct
    15061 ttgtatagaa atgtatgcta acgagggggg ataaatatgg aattaaaaat tgacaaactc
    15121 acgcttgatg atccagcttt taaaattatt gacgatcgag ttgttcaaca agtagagcaa
    15181 actcaaggaa cgttactttc attattagaa aaaaagtacc agtttccaaa atatatgaac
    15241 aaggcacaag ctgctaagta tatgaacgtt tcatacaata ctatgatgca gaaatatgtt
    15301 cctaacggtt taaagttgat tatagttgat ggagtcgtaa gaatttcaca agatgaatgt
    15361 gatcgcttta tggaagaata ccaaaaataa acgcaagccg tgggggcaaa gggattaata
    15421 ggaggaaata ataatggaat cgacacaaaa taaagcaatc gaaaaagtat tatcaacaat
    15481 tcttacagaa gacacagcgg cagaattagc aaacttggaa ggcaaagcgc tagaagaaac
    15541 gtttgaatgt ttgtacgaac aaatggacta tcaaaaattg ctaccacaag aaccaacggt
    15601 aagcggagta ttgcgtggct tgaacgattt agtacaagca gagtttaaag aacgactttc
    15661 tattgaagag tatcaagaaa ttttgtatca gcaagttgag ttgttagcga gcttgctagg
    15721 gcttgaactg gaggacgttg aatgaaaact gaaatttgga acggacacat tataagattt
    15781 gtcgatatta atgatgaatg gtgggcagtg gcgaaagatg ttgcggaagc gttaggtctt
    15841 aaacaggtta ctagagccat tcactctttg cctaaagatg gggttactac tagtaaggtc
    15901 atcgattcat tgggcagaac acaagatgta aacatcatca acgaaaaaaa atatttaccg
    15961 catggcattc aaaagccgta aaaaagaagc cgaggcattt caagactgga ttttcgacat
    16021 catcaaagaa ctacgccagt ccacaggact tgaaggcttt caagtattcc gcatgctaga
    16081 caaagaacac caaaaagaag caatgactaa attaagccac gctattactg aaccaaaacc
    16141 agtagactac attaaagcga atgtgatagc caataaagct gtatcaacta tttatggtca
    16201 ttccaaaatg gtaaaaaaga aagatatgac tcctgaaatg ttggttgatc gtgaacctat
    16261 tttagatgag acagtagaat taatgactgt taaagaaaaa tatgggttgc agttttcagt
    16321 gagtgagaaa atttataatc gttctgctga attgcagaca acttaggagc attaacatga
    16381 tcgagaacaa agaaaaagcc ttatccataa cagtcttggc ggacaggata aaggcaactt
    16441 aattgggata aaacccgata ttctttgttc cgattatacc atattaaaaa atcggaggga
    16501 accattatga aacgaaactt aattatagga agcattttaa tcggcggaat tttcggctta
    16561 ttaccattaa ttgcaaaaat agctatcttt gcaggattag cgttatatct cttcactgct
    16621 tatgacgagt acgactatca attaagaact ggagggcata agtagatgta caaaacaaaa
    16681 ctattaaacc aactcgatag ccttgaatta gaggaaatta atcaagggat cgctgaacta
    16741 gaaaataata tcgggaaaac ctactttgga aattcattca atgaaaaact aacagttttg
    16801 tatgtactca aaaaacatgc agaacacaaa ataatttgtc gagaaatcaa cgagctaaaa
    16861 aatcagattt taaccgcatg gttaaacatt actgatatgc aagaagcaag ggtgaaaaca
    16921 tttaatacat gggtaaagta tcagaatcaa ttaaaaggtg ctgaatttgt tcgggatggg
    16981 ttgaaatacg aacttgagca gttgaagctc atggaggtgt ctgaatgatt tataccagta
    17041 ttttgaaaaa ggaggagagg tttattggca aataattatc aagcagcaac ggaattacaa
    17101 aagtcgggat ggtcaattta tcccttatct cctggaacta aaattccaat aaaaggctct
    17161 agtggttgga atgatgcaac taacgattct aatcaaatta atgaatggtg gacggaagat
    17221 gcaactagaa atattgggtt aatgcttgaa gatcatcaaa tggtggtagt tgacctagat
    17281 caacattctg aaacacgaaa cggcgttgag aattataaaa aacttgcttc aatgtatgat
    17341 ccatttcctt ctacttatac ggaaatcaca ccaggtaaag gaatccattt cttctttaaa
    17401 attcctgatg gtgtaattat ccaacaagaa acaggtgcat ttgctgatat cttcgggcgt
    17461 gatgataaag gtaaaagttt aagcgggatt gatattatca ctaaaggagt tccaatagcg
    17521 ccaactatga ttatttcaag aagtgttggg agagcatatg aacctattga aaacgtatct
    17581 ctaaataata ttgcaccagc tcccgattgg ctagtgaacc tactgctaaa aaaaccgcaa
    17641 gaaataaaga ctcaataccg acccgtagca aaaacaggga cagcaaaaaa attagatatg
    17701 attgttcaag gcgctggcga aggtagtaga aacgatcatt taactaagtt atgcggttgg
    17761 ttactatggc acggtgtaga taatcaaact ttagcagaat tgatttatac agcaaatgcg
    17821 tacaatgaac caccgctaaa agataaagaa gtgaatcaaa ttattcgatc gatgattaaa
    17881 aaagatttga gggggaaagc tcatggctca taagcataaa ttagatagta ttttagactt
    17941 tcccgaagcc agcgagcgtg aagacaatat cattgaattg aaaacctgga tgtcacgatt
    18001 aagatgcaat aaagacgatc agattaaaag taacagtgtt gtgaatgcag aattgatttt
    18061 aaccaatgat agcaatttag ctggaacaat tgcttataac gaatttagcg gatacattca
    18121 cctattaaaa gattctcctt ggatcaatcg taccgctgga gaatgggaag acagttttga
    18181 ggatgcctta acggcatata ttgaagaaaa ctacaatgta gtttttgatg acaacaaaat
    18241 acacaaagca gtagtaaatg tggctagaaa gaatgttttc aacccagtaa aagaacgtat
    18301 cgaaaaagtt aaatgggatc aacagcctag actggaaaca ctgtttattg accttttagg
    18361 ggtagacgac aacctttaca cacgagaagt aacaaaacgt tggatagtcg gatcagtcgc
    18421 acgtatctat aaaccaggaa taaagtttga gatcgttcca gttttggatg gaccgcaagg
    18481 gattggtaag agtaccgttc cagcattact ttacacggat gattttttca ccgattcatt
    18541 agattcgcta ggtgaaaaga aagatgatta tatgcagctt caaggcaatg taattattga
    18601 acttggagaa ttatcttcaa tgaacaaaac taaaattgaa cagataaaaa acttcatcag
    18661 tgccaagatc gataaaatta gacctcctta tggtcggaat gtcatagctt gggcaaggca
    18721 atgtgtattt attggaacgt cgaatgatgg gcagtatttg aaagatgata caggaaaccg
    18781 ccgtttttat cctttgcctt gtaagaataa gccaaagatg aatccgttta aaacaaagga
    18841 tgaatatttt cttcaagtgt tagctgaagc aaaggtgctt tttgataaag gacaacgaat
    18901 ctattttagt cccgatgaag ataaagaagt cttagaaatt gcaaaagact atcaagagga
    18961 tgccaagatg gagaatccaa ttaaagaagc aatcactaaa tatttggaaa tggaaatccc
    19021 gtataaatgg gaagaagctc cagcgtgggc gagacgttct tattatcaga actatcctga
    19081 cagtaaatgt aatgagaaaa atttacagca tttctataat ggagctacta tgcaacaaga
    19141 gttttattta gttgatagcg tactcaccgc tgatattttg gaagctgttt ttgataaaca
    19201 agcaaaggat ttattgaatg gtcgatcaga tgcagaaact aaaaagattg ccttgattat
    19261 tagcagtatt cctggttggg aacggaagca actgccaaaa agaaataaac gacgaggatt
    19321 ttacaataag tcaaatgcct atgaaaataa aaatcgagct ggaaagtaca aaaagtaacg
    19381 ggtagtaacg ggtcagtaac acctagcccg ttactagttt acccttactg ccccaaggga
    19441 tacagcaatt tggtaacggg gtaacacctt atttccttaa aacttttgaa atagttagct
    19501 aatatgctgg ggaataatgt tactggattt tacccgttac ctgttaccaa aacgctagaa
    19561 cccttgtccc cgttgggatt gagtggtaac gggtacctgt tacgtaggtg ttaccacgtg
    19621 ttacgaatca aggaggaaag taaatgctca atattatttc aacaaacaaa gcaccgaact
    19681 ttcaatatac agatgaaatg gatcggtttc taatgaacac gctggcattt agtgtgggat
    19741 tggtaacaga agattattcc acatttgatc cagaagtatt aaagataatg gaagaagaac
    19801 ctgattggct gcaggagtca gtagcgtggt gtcaatcgct agtagtggga tcattggtag
    19861 atagtggcaa ctatgatgat acaggtgagc taatggatga atttaattgt ctgcttaacc
    19921 tatatgatcg agcgagacaa cgagagctta catcgaatga ggataatttg tttttaaaca
    19981 tccatgataa gttcttggct ttgctactaa cagatgatga attgataact aatttattgg
    20041 aggtagaata attatgcata tggaaagtat tagatacttc gatggtgaga aacacattga
    20101 gtattcaaac actcaagaag atgtagactt tattagtttt gaggaagata aaatcactaa
    20161 agttaatttt aaagacggaa cgtgcttgaa gatagtttcg ccttatattg aatacaaatc
    20221 aaaatgggaa caagatgatt ctgacgaacg agatcgcttt ggttggtaat ctatggcagt
    20281 taaaaaacaa tgtaatcatg ctggatgtaa aacattgatt gattataggc agaagtattg
    20341 tgggaagcat aaagcaaagc aaacggcaat taagcgtgaa gaaagaaagc aaagcgaagg
    20401 taaatatttt caattctatc aaagtagaac atggcgaaag gcttcatact tgtatcggtt
    20461 aaatcatcca gtatgtgagg attgcctaga agaaggcttg ataaggaaag ctgatgttgt
    20521 agatcacaaa attgaactaa aagatgattg gaatagacgg ttagatgaaa ctaattttcg
    20581 ttctttatgc catgcacacc ataattccaa gacagccaat gaaagacaaa gacgagaaaa
    20641 gagtaccctt tgagttaagg gggcatagtg tgaaacttac tgacaatcga tgcctactca
    20701 ttttggtaca aataaccgtt gtgaaaaggc ataagggtaa ttacaaataa cgattacact
    20761 tgtaatcaca agtgaaaaag tatataatga aagtaggaga tttattgaaa aatacaattg
    20821 ttattactga taaaacaggg aaacagcgca ccattgagtt acctcatttt ggtcaagtga
    20881 ccattcaaat gcagaacgga aaaatcattt atatagataa attagataaa gaaaaattct
    20941 gatcgaaaaa cgaaggaatg tgagcttaat tgcttacgtt cctttttctt ttgtctgaaa
    21001 ggaggacaac atgggacacc ctaaaatatt acacgataca aaaggcaata tatcaagtga
    21061 agaaaaagca atccgtgtgg acgctagaga agaattgttc aagcagcaac cattaataaa
    21121 tatcacgccc cctgactgga tggcagcgag cgctagaagt gaatggaatc gtatcgtacc
    21181 aacattaaaa aaagattatc cattgagtga agcagattat ggttcattgg tagcctattg
    21241 tttagccttt gctcgaatga aaacagccga agccgagata agaaaatcag gtacgtttat
    21301 cacatgcgaa aacggagtaa agaaagccaa tccagcggtt cgggttcaat cacaagctat
    21361 gagtgatttg aaaaaacaag ccacctcact aggtatgacc ttagaatcac gatcaaaact
    21421 agctttgaac aaggttaaaa atgatgaacc cgaagacccg ttcaaagagt tgatgaattc
    21481 atgaatgatt acattgagaa agtcctatca ggtaagctga ttgcacccaa gaaaattatc
    21541 caagcgtgtg agcgtcatat aagcgatttg gagcgttcta agtcagatag cttcccttat
    21601 gtgttcgatg aagaacaagc caccaaagcg attaaattca ttgagttgct accatctacg
    21661 gacggtaaag caatcaagat gttaggattt caaaaattca ttctaggcag tctttatggc
    21721 tggcgtacta aagaagggaa ttacaggcga ttcaatcgag cgtttaccag tatgagccgt
    21781 aagaatggga aaacgtatat cgcaagtggc atggctgcca atgcgttgat tatggaacaa
    21841 gaaccagcag aagcaaggca agtattgttt gtaagtaacg ctttgaaaca agctaaattg
    21901 ggctatgata tgctgtctaa ttcacttaga aacgtggtca agtctagtaa gtttttaaga
    21961 ccacaactga aaattatgaa ctctaagatt cagcacttgc cctctaattc gttcgctatg
    22021 gcactggcta gtgaaaccag cacgctagat gggtttgcac caacaaccgc aattcttgac
    22081 gagtggcacg aagcaaaaac tcgtaaaacg tacaacgtca ttaagtcagg aatgacccaa
    22141 caaaagaacg gcttattgtg tgttattagt accgctgggc ttgatttaaa cgttcctatg
    22201 tacgaggaat acttattgtt agatcgtgtg ctaaaaggcg aagaacaagc cgacaggtac
    22261 tttatagcga tatgggaatt ggacgatccc gaagaaattc acgatcaaga gaaatggatc
    22321 aaagccaatc cgatttttga aagtgaagaa atcaaaaaag taatgattcc aaccattcaa
    22381 gatgatgtga accttgcttt gaaacaaaat aaccttaatt ctgtattggt gaaaaacttc
    22441 aatttatgga gacaagcgag tgaggacagc tatatgattg ctgaagactg gcaggcaacc
    22501 gaagtagaac cacaagatat tacaggcaag cccgtttata tcggagtgga tttatctaaa
    22561 acagatgatt taactagcgt ttcatggatc gtaccgctag ataacggcga actttattgt
    22621 gattcacata gctttgtagc caccaaatat gggcttcaag acaaagaaaa gcgcgacggt
    22681 ttgccttatc gagaactaga aaaagctggt gagtgttcca ttactcaatt agaaagtgga
    22741 atcgtggact atgaccaagt atttcagttt attcaagatt tgattcaaga aaatgatttg
    22801 gaatgtatgg ggatttgtta tgacccgtat aacgctaatt cgcttatcag taaagctgaa
    22861 aaagccaact acccaatgtt agaagtaaga caaggaacga ttactctaaa cgttccgacc
    22921 cgaactttta gagaacaagt ttatgaaggc aacgttattc acaataaaaa tacgattctc
    22981 acccatgcag tgaacaacgc tattttaaaa acggataaca acggcattca gattaataaa
    23041 tcaaagaaca gtaacaaaat tgatccaata gctgcattaa tcaatgccta tgtgtttgca
    23101 atggattact tcaccacaac ggaaggagcg aaagcagaca atgaatttta tacaagtgaa
    23161 gaattttctt tctaattaca ttcataccgt tcttttactt cttggattgg tgtgtgtgtt
    23221 ggttgctatc accttactaa caaatgtcta ctatggcttg ttagcgctgg gcatagtgct
    23281 tattggggta gcggtcatgc taaatacaga acagaaggga ggttaaaaga tggcattttt
    23341 taaagagaga caaaatacaa cgggagatcc tttcctacaa catgtggtat caatccaatc
    23401 ggatgattac accaccagct ttacaagcgt tcgtgcatta agaaatagtg atgtgtttgc
    23461 agccgttcga attattgcta gtgatattgc ttcaagtccg attcaattgg tcaaaaacaa
    23521 tatgccacaa gctgataatg aactggtgaa gttactaaac gagaaaccta attcagaaat
    23581 ggatggttgg catttaaagt ttgctttggc ggtcaatatg ttgctaaacg gtaatagctt
    23641 tgcagagatt aaacgcaacg gtgaaaaggt agaagaaatt cacttattac ctaactcaag
    23701 tgtaacggtt actcaattag ataatggtac gttgtcttat cagattggtg ataaaaaaag
    23761 acgtgtgaag tctagcgata ttttgcactt caaatatttc acccaagatg gtttaacagg
    23821 attaccacca ctttatgcgt tacgtgatga actgaaaatt caacaagctg gcaaccgcac
    23881 attgcacaat ttctttactc gtggtgtcag tgggtcaggt attttgaagg ttcataagtc
    23941 agatttagac ggatcagcaa agaacgcaat tcgtgaaaag tttgaagaag ccaacggttc
    24001 aagtagtgga gataatgctc ttagaacgat cattcttgat gaaacaatgg actacaaaac
    24061 attagaaata aacaccgatg ttttgaagct tatcaattcc aatgattgga acacaaaaca
    24121 aattgccaag gcgtttggtg taccgattga gcgtttaggc gttgaaaatg aacattctag
    24181 caccgttcaa agtaatctcc agtatatcca aagtacgctg attcattact ttaatgtgtt
    24241 tgtgagtgaa ttagatacga aacttgaaac caatatccgc tttaattccg atcagttact
    24301 agagactgat cctgaaacaa aagtaaaaaa tgtattggat caggtcaaag ggtcacttct
    24361 cacgattaat gaggggcggt cgaaaatggg gctacccccc atggatggtg gcgatcgttt
    24421 actagcaagt ttgaacttta cgtatttaga tacgttggag aaatatcaat taaaagaaca
    24481 ggaaggagtt acaccagttg aataatgaag aagaaaagga aaaacggctg acagaagaag
    24541 ctgagctaac agccgattct cccaaagtag agaaagaaaa tgaaaaacaa ccaacagacg
    24601 gcaaaactat ttcaggctat gcgttgaaat tcgggcaacc gtcaaaagat ttaggcggct
    24661 ttgtagaagt gattacaccc gaagcattaa aagaggtgga tttatcaaat gtgttcttat
    24721 tatatggaca cgattattca aagccactag ctagtgtcaa agcaggtacg ttgaaattga
    24781 atgtcgatga tacaggatta cattttgaag cagagctaac cgacacgacc tattcaaatg
    24841 atgtgtatga gaatatctca aaaggggttg ttgattctat gtcctttggg ttcgtgcttg
    24901 gattagattc attcgacaaa aaagaagatg gcacaattga acgatcaata gataaaatca
    24961 aagcacttaa tgaaattagt gtcgtgaccg ttcccgctta tgattcatca aatgtccaag
    25021 taaataagcg ttcttacgaa tcgtttatga gtaacaacca agcaaaacaa acaaacaata
    25081 gcttagaatc cacttctaaa gcacaaaagg agagtaataa catggaaaaa actttaatcg
    25141 ataacgaaaa aacagaattg cgtgggtatg aagaatatat ccgttcacaa ggcgaagtgc
    25201 gtgatggagt cactactgta aatgcagcgg cagttgttcc cgaagaagta atcggtgaag
    25261 tctttgattt gaaacgttca aattataact tagctcaata tgcaacagta aaaacagtat
    25321 caaatggaca aggtaaatat ccagtagcaa ctaaccaaca agcagtgtta gcaacaaaag
    25381 ctgaacttgc tgaaattggt gatattgacg ctgaaatgtt tacttcagtt gattataaag
    25441 tagaaactcg tgctggtaag attgccttat caaatgaggt tgtggaagat tcagcagtga
    25501 atattgtaca agaggtcaaa gatcagttag cgaaattggt agaaaacact gacaataagc
    25561 atatcatgga tttattaaaa acattcacta agaaaacggc tgctacgttg gacgatttga
    25621 aacaactata caatgtggca ttagacccag cattaaataa aatggtaatt ctaaaccaaa
    25681 gcggatatta ccacctagat acattgaaag attcagatgg acgttacatt ttacaacccg
    25741 atgtgacagc acctagtggt aaatcattat tcggtatgcc agtagtattg attgcagata
    25801 cattgtttgc caatcctaaa gctggaacat tccctatgat tatgggggat attgcacaat
    25861 ctatctttgt tgctcgtaga aatcaagtaa cgactcaatg ggaaaaattc gattactact
    25921 cacaaggact tgcagtgatc gttcgcaacg attataagaa aattgatcaa aatgcttcag
    25981 tgtatattga gtttacgcca gttgtaacac caaaagtata gaaaaatatt gggcggcggt
    26041 ttatcccact gccttttttt cattaggaga gataaaatgg taattttgga tagcataaaa
    26101 aaaagtatgc ggatcgatca cactattgat gatgacttta ttcaacaatt gattgataca
    26161 gcaggcgaat atataaaaag tgctattgat agtagcgcaa cggataaaga tatggataat
    26221 tatcagcaat ttgatttggc ggtgtcatta cttactcaac attggtattt gaaccgtcaa
    26281 gaagccagca gcgaacggat accagtaacg gtacaagcat tagtacaaca aatgagaggt
    26341 gcttattatg ccaatcatta agaatgtaaa tgaattgact gagagagtca agtttaaaaa
    26401 gacaaaacgg gtaaaagatg aagatgggca gatggtggat agtgaagaaa ctgtatttga
    26461 atgttgggct aatgtgcgtt cacaaatgct aaaagacgtg cttgctagtg taggtaccat
    26521 tcttgaagga acgttgacgt ttattattcg atacgatcaa gattatgaac taacaaacga
    26581 tatgaaagta gcttggaaaa acaagagtta taaaattatt tcaatcaatg aaggaacggc
    26641 gtttaaagat tatacaacga tcatagctaa acagatttct tagaatgatt acacttgtaa
    26701 acgttgtggg tatttagtat aattaaggta gtaaatgaag ggttagctgc ctgaattttg
    26761 caagacttag ctagtcgaaa tctattgttg gactgaaaat tgtaggtgtg gttgcaaaca
    26821 tgctggacta agaaagatga tagatgatga acgttcaatt ttaatacagg catgctagga
    26881 ctgattggat ggttttggca agctgattgt gtagaaatca aagaacacgt ctgttgtgga
    26941 cgtgtttttt ttgttataat tcaaaagagg tgatttaatg gacaaagcag agtttacaaa
    27001 tttttattgg aagtattatc tacatttaga aaatgagttt attaatacca cagattttgt
    27061 gatgctcgat gagaataatt tcaaaacatt ctctattgaa tatcaaaaac tactactagc
    27121 gattggttcg gagtgtgaga ttattttcaa agaactttgc ggatttgata gcagtagcaa
    27181 taaaagcata actgatttta aagctgttat taaatctagc gaattaatgt gtttggataa
    27241 tggtgttagg gttatgaatt ctttaacatt gacttcacta aaaccttttg gagatgggtg
    27301 gcctgaagat acaccagatt ggtggaaaat atataacaga gtaaaacatg gtcgttcttc
    27361 aaattataag gaagcaaatt tggaaaatgt gttgtatgct ttagcaagtc tatatttatt
    27421 agaggaatat ttgcataaga aagtaatact agaaggtgaa gtagacttta taagtccaga
    27481 gtcaaatcta tttacgttat cttgggaaag aaaacattcg tcgatgcaaa aagtgacgtt
    27541 tgaaaaagtg gattcaaatt atgtagctcc actcattgat tttaaagaac aagaagaaat
    27601 ttaaaaaccc ccacggctta ttgaagtttt tgtatgccat attgtatgcc atttggttta
    27661 aatcgcttga aaaatagtga tagataactc taattcattt agtcataaac gctgttttga
    27721 taacctttat agttctttga tatgggtttc tcattcatgg cggaagaaga agaatagaac
    27781 aaaagaatga attaataaga tataaaaaaa gggattgtga caaacgtctt gtcacaatcc
    27841 ctttttccaa ataaacgatg ttcaatcagc tgcccctcgg cattcgctcg aaaaacggcg
    27901 aaatatgaag aactattttg ccatttgttt ctctaataat caggtcaaaa cgttgacttc
    27961 acagtctctt ccttaaacgg tgttctcctg ccaactcctc ggtcacaagt cacaatattc
    28021 aacaaacatg agaagctgtt ttttgaaatt gttccttgtg actcggagct gaacggcagg
    28081 ctcacgacct ctttcttaaa cggtgttcaa aagcttgcat ctgcggtaat aagttcaacc
    28141 aaacgaaaat tatgaggagc tatttttgtt tggctgttct tattacttga tgcaggacag
    28201 ctttttcaca acctcttttc gtttagcact aaatttcctt aaaaagtaga aaagctttcg
    28261 aagcctcgtt tgtttctttt actgtataat aaaagaaaag agtaacaatc gctaaaagat
    28321 ataaacaaga gaaaggaaat acaagatgat tggtctgatt tattttgtgg tgatcgttct
    28381 ggcgaacaca gttggcgcta tttccgggat gggtggaggc gtgttgatca aaccgattct
    28441 tgatcttatc ggcgcccatt ctgttgcagg gatttctttt tactcaacag ttgcggtatt
    28501 cacgatgtcc attgtttcga ctgtgcgaca agtatcaagc gggaaatcac tgaattggca
    28561 gattgtcggt tgggtatcta gtggagctgt tgttggagga gtagtcggca acattgtatt
    28621 cgaagttttt ttacagttat ttgaaaacga gaagcatgtg cagctgatcc aaattttttt
    28681 gacggtattg accttggttt ttgcgttctt ctacaccaag catcatcagc caaaatttca
    28741 tttgacgtca tggacttggt accttatatg tgggggggtt ctgggctttt tagctagttt
    28801 tttaggtatt ggtggtggac cagtcaatgt ttctttgttg atgctgatgt ttgcattgcc
    28861 aataaaagaa gcaacactat attctctatc tacgattttc ttttctcaac tggcaaaatt
    28921 ggtaacgatt gctttaacaa gtagctttat gcgctttgat ttatctatgt tgttttatgt
    28981 tatccccgct gctgttattg gaggtttgtg gggagcaaga tttagcaggg ttttgtctcc
    29041 aaaaaaagtg acctttattt ttcaagtaat tgtcctcgtg gttcttttgg tcaattgtta
    29101 caatgccttt gtcatcttac aaacattagg agaatctaga ctaacgcaag tcaaaatcac
    29161 tgaaacatat tgttctaaat gaaaaaaaga gtatactatg ataaaaaaat tcattttaaa
    29221 aaatcagaaa ttggctggtg atttggatgt tacgggaaat gcgtttagaa gatgcagaag
    29281 cactacgtga aatcaatgcg gatcaattag ggtatgatat accattaacc gttacggtga
    29341 gacaaatgaa gaaactgctg aaggagccag agaaaaattt tttcttggtt tatgaagatc
    29401 ctacttctaa aaaagcagct ggctatgtac aagcggagtc ctatgaatcg atttttagcg
    29461 atccaatgtt caatattatg gcacttgctg tgtctaaata tgctgaaaaa agaggcatcg
    29521 gaaaggcttt gatgactggc gtagaagaag aagcgaaaaa acgaggaata acgacaataa
    29581 ggcttaattc tgcagaatac agagtagaag cacaccaatt ttatgaacac atcggttatc
    29641 attccgataa aatgcaaaaa agatttttga aaatcctgta aatctttatc aaaaaaatac
    29701 ccgatcacca agccgatact tgatgatcag gtatttttgt ttttttgact aagaaagtaa
    29761 ttgcatcaag aaaccagaga tgatcaaaac gatggcacca ccaagtcgat tgcccatttg
    29821 agcaaaagca ataagttcca tccgattagc tgcagataag acggctacat ttcctgttcc
    29881 gcccatcgag ttgttgcata agccagcagt aatagttgat tcaattggat ataaaccaaa
    29941 caaacgaccg acaaatcctg aaacaactga aatcgcgata acacttgtca gacataagac
    30001 aacaaattgc catgtcaaag cagaagctaa tacattcaga ttcagcaacg caataccgat
    30061 accggcaaga acagctgctg tcaagttttt gacaatcaag ttattgaaca tgatagctgc
    30121 atcttcataa tactctggta ataggttggc gattttacag atgacaacga taataatcat
    30181 aaatgcgtaa gcatggactt taggaacaaa atagttacca atttgtccta agatgaagaa
    30241 agtcaatgat accatcaaac caacacctaa ctgtggaatg gaaggtttca cagttcgagg
    30301 ttttccatcg ccaagatcgc ctttttccat taatttacca ttgccgttga attttggcat
    30361 tgattgtcca gcacgagcaa ctaatgctgc accgatgata gctaagacat ttgtcattgc
    30421 tgtcgcagga atcaggcggg aaatgataga agaagcatcc gtacctaaaa catttgcata
    30481 aatcgttgat aaaggaacgg ctccggctcc aacaccacca gccatcgcag gtaatgagat
    30541 gtataaaacg gaatttgcaa aaccatttcc gataagcatg cccacgagtc ctacggcaaa
    30601 aaaggagacg accattgaaa taagtgcaac gggaataaaa cgaacagaag ctttcatcaa
    30661 tagattacgg ttcataccaa ggatgctacc agtaatcaag gcagcaatat aaaagtccca
    30721 aaagcccata ttgctgacaa agttttctgt ggatttcact acatattcag gtaagatccc
    30781 aaatgtagca atcgcagcag aagcgaagat cgcaaaaacg gaaccaccac ccaaataact
    30841 tttgacgata gggatttttg tcccgataaa atgaaataaa ttaccgaaaa taaccaaaac
    30901 tgcgattggt ccaatcatat tcgtcggtaa tttccctaaa gccattgcaa tgatcaggac
    30961 aaggaccatg atcaaataaa gtggcaaact tacgccggtg atttttgtgg accaaaaacc
    31021 ttcttttttc ttttcctcaa ctgcagttgc agttgttgtt ttttgtgcca tttttacaga
    31081 tcctttctta ttctgtatca tatgaaaaaa gccaaagaaa agcgacaaaa gagcaggagt
    31141 tggctggccg ctgttttgcc gcagtcgctt ttttcttttt agacgtcata caaagtttct
    31201 gtatgttctt ttactataac gatcttaatc ttctaaagat ttatattcag gaacccattt
    31261 agcagattca actgcttctt tgatatccgt gatttcttca cgattcaagt tttgtgcgac
    31321 aactgatttt gcgacagttt cagcaatcgt ttgagaaaat tcagtgattt ttgctactgg
    31381 aggcaagata gctgctcctg gttttgttac atcaacgatt ccgcctaatg ctcgactagc
    31441 ttgagaaatg atttcagcat ttactcgtgt agctgttgaa gcaatcaaac caagtcctaa
    31501 acctgggtac atcaaagcat tatttgcttg accgatttgg taagttacac cattatattc
    31561 aacatcagca gcagggatac ctgttccaac taatgctttt ccgtccgtcc attctattaa
    31621 atcttttgct tttgcttctg ccaattttgt tggattagac aaaggcatga tgatcggacg
    31681 tggtgtatga gcagccattt ctttcacgat ttcttcagtg aatgctccag gttgggtaga
    31741 agtaccaatc atgattgtcg gatggatcgc ttttacaact gcttctaagt ttgtcaattc
    31801 atcagcatta gtgaaatctg agcgtttgcg agcgaatggg atttgaccag gagtcaatcc
    31861 ttctgtatct tcaaacaata aaccttgttt atctacttga tagaaatgtt ggcgtgcttc
    31921 ttcttcaggt acaccttgac ggatcatctc atctaaaagg atgtttgcga tacccacacc
    31981 agcagtacca gcaccaaatg tcaaaattat ttgatctttc aattgttcac cggatatatt
    32041 caatccgcct aatacacctg ctaggacaac aattcctgta ccttggatat cgtcgttgaa
    32101 agtagtgatt tgatcttcgt atttttctaa gatcgctgca gcatttcctc gtccaaaatc
    32161 ttcccaatgc aataacaatt ctgggaataa ttcggtagct gattgaacga attgatcgat
    32221 gaattcatag taagtttcgc cttctacacg agcatgacga ttgcctaagt ataagggatt
    32281 attcaataat tcttcattat tagtaccagc gtcgatcgat acaggcaaaa cttgtgctgg
    32341 attaatacca gcagcagcag tatagaccat caatttgccg atagcgatgt caacaccgtt
    32401 cacaccccag tcgcccatac ctaagattcc ttctgcatca gtcacaacaa tcaaacgaat
    32461 gtctcgtcca tctgccgcgt tcttcaaact tgctttgatg ttttctggtt cgtcgattga
    32521 aagaaatgca gcatcttgcg gtttcaagaa aatttcgttg tattgttcga ttgaatccgc
    32581 aacgacagga tcataaacga caggcatgaa ttcaactaaa tgttcgccca tcaatttata
    32641 aaacaatgtc cgattcgtat tgaatagatt cattaagaag atacgttttt ccaaatctgt
    32701 ttgtttgctt aagtattgac cataagcttg aacagattgt tgttccagtg tttggacagt
    32761 acttggaagc ataccagtga ttccatattt tgcccgttct tcttttgtaa aagctgttcc
    32821 tttatttaga aaaggattat ttaataagtt taaccctgta agcatgaatg attcctccta
    32881 agttttaata caaacgaagt atagaagtca gatgatgata tagtcaaaaa gagcaggtat
    32941 gataaatttt atttatatgt aatatagcca aaagaaatca aaatagcagt atttgttctt
    33001 tttatatcga caatccttgc ttgaatacta tgcgtatctc ttatatgcta gagaaagaaa
    33061 agtagaagaa taagggatag atggaatgga taaataggat ttctgaggaa aaaagcacat
    33121 gatagcgttg ataggagtca cctatgtcag gtgataaaaa tacataagta gaaaattaag
    33181 aggaggaaga gaaaatgaaa gaagttcaat tgattagaaa aagcgaacta tcagaaggcg
    33241 gttgcaatgc atgtggcgta gtagaagcga caagctatac attaaaatta gattccaatc
    33301 aagcaataat ctctgaactg acagtaggcg gtcttgtgga cagtctggcg cttgctgaag
    33361 gttttactgg agaagatatt tatgagatgt tttcagaagt ccgtcagtta aaaaaaggag
    33421 aaaaatgcat agaggttcat catgagagcc cgaatgtgag gttcaaacgt ggtgataatg
    33481 agatgatctt caagaatcat gtgtctgatc acactgaact atatgaaatc gtgaatcaaa
    33541 tactgtctgg gatttttgaa ttaggacctt atgagttcaa ggaagaaaac ggtaatccaa
    33601 aattaaatga agaatggcaa gaaacgattg aaacacaaag aaacaatcca cacttatttc
    33661 aatagtcttc cacagtattt cgtaaaaatg ttgatgaaaa agaaacaccg tgaatcagag
    33721 gttgtgaaaa agtcgcttag tttcgagtag taagaaaaat tttgtgaaaa tggctttcca
    33781 catttttgca taaatttgac ttaataccga gaaaatggct tttgaacacc gtttattcaa
    33841 aaaaagggac tttgacacta cttttgtcaa agtcccatcg gttttttatc tatttttatt
    33901 acaatcattt cttccagagt agtgacatct ggaatggctg aaatgtaaac agattatttg
    33961 cctgtatcaa tgtcgaagag taatataaaa agtgtaaacg ctatcatatt gttcatcatt
    34021 caaattttat agagagaagg cattgagatg gaaaaaacat atcaagcagg tacaaatgaa
    34081 ggaatagtag attttatcaa tatggaagat ttagaaatcg cggcttcaca agtgattcca
    34141 gctggtggtt atggttacat cagcagtggt gctggcgact tatttactta tcaagaaaat
    34201 gaacgggcat tcaatcatcg tttgattatt cctcatgtat taagagatgt agaattaccc
    34261 gatacaacta cacattttga cgaagaaatg ctgactgcac caatcatcat ggcaccagtt
    34321 gctgctcatg gattagctca tgtgaaggcg gagaaagctt ctgcaaaagg tgtagcagat
    34381 tttgggacga tctatacagc aagttcttac gcttcttgta ctttagaaga gataagagaa
    34441 gcaggcgggg agaaagcacc gcaatggttt caattttata tgagcaaaga taatggaatc
    34501 aatttggata ttcttgaagt ggccaagcgt aatggagcaa aagcaatcgt attgacagcg
    34561 gatgcaacag ttggcggaaa ccgtgaaaca gaccgcagaa acgggtttac cttcccgctg
    34621 ccaatgccga tcgttcaggc ttatcaatca ggagtaggcc aaacgatgga tgctgtatat
    34681 aaatcttcca aacaaaaact tagtccaaaa gatgtagaat ttatcgctgc tcattctgat
    34741 ttgccagtct acgtgaaagg tgttcaatca gaagaagatg tctatcgatc attagaatct
    34801 ggtgctggtg gaatttgggt atctaaccat ggtggccgtc aattagatgg ggggcctgct
    34861 gcgtttgatt cgttgcaata tgtggcagaa gcagtagata aacgtgtacc aatcgtgttt
    34921 gacagcggtg tacgacgtgg acaacatgtt ttcaaagcaa tcgcatcagg tgccgatctt
    34981 gttgctattg gcagacccgt tatttatgga ttgtctttag gaggaagtac aggagtccat
    35041 caagtgtttg atttctttaa aacagaattg gaaatggtca tgcaattagc tggaacacaa
    35101 acagtagaag atatcaaaaa aataaaattg cgtgagaatc gttttattta agaaataaat
    35161 gacgaaaaaa ataggagtgc ggcagatgat gccgtactcc tatttttata gttcaaggat
    35221 gtcactaata gaataatttg aaacgaaaaa ataaaattga aatatttttt catacttaat
    35281 aaaaaaggaa gaatattcat gtaaacgatt ttattgtttt tttctctttt atacaaaaag
    35341 ggtcataatt tcaagtaagc ggatgaattc atgaggattt tttgcatact gtctctacta
    35401 tacttaggtc actaagaaaa aggagtgatt tattatgaaa aagaacatga ttgggacatt
    35461 attactttca acacttcttc taggcggctt ggcaacacca gcttttgctg agggtcaagc
    35521 gacttcaaaa ggagatatta cctttactga accaacaaat accgttgagc ctcttaaccc
    35581 tactgatcct tcaaaaccag tagagccagc agatccggaa aatccagcaa ctggccagac
    35641 aggttcttta acattggatg tagttcctga attgccattt ggtacacacg agattgaaag
    35701 cggaacaaaa acgtatcaag tagacgcttc aaaaaacgat acaccttatc tccaagtatc
    35761 agatcgtcga ggtgtaggtg cagatggcca agcacaaggg tggaacgtga cagtttcggt
    35821 ttcagatttt gtaaacggtt cacaagtttt gcaaggcgct gaattagatt ttggaacatc
    35881 cactgttaaa agtacgtcag ataacgaatc cactggacca actagtcaaa ctgtaactgg
    35941 gttgagtaaa gcaagtgcag cgacaccaat atttacagca gcaaaagatc aaggtctggg
    36001 aacatggctt tctgtttatg atccagcaaa tatcacactg aaagtgccga aagcagcagc
    36061 tggaacattc acggctgatt tgacttggaa cctcgtggct ggcccagtag catagagtat
    36121 gaaataaatt caacaaactg cggtgaaaat actataggtg aaggattttt taccgcagtt
    36181 gttgttgaaa ggatgtggtg gacaatgaat caaagtaaac gactgtttgt ttcgtttttt
    36241 tctattttaa gtctattttt catttttccg tcaatcagcc aagcaaagga ttcagcagga
    36301 gactttggaa tcaaaccggt atttccagag aaccaaatag ataaagcaat cggttatttt
    36361 gatttattag tggcaccgga acaaaatcag acactagaag taattatcag caattcttca
    36421 gatgaagaac gaacatttga ggtctctgtc aatccggcgg taacgagtga tggtggaacg
    36481 atcgattata gtcaaaaaaa cccaacgtta gatgagacat tgccgtttga tgtccgtgac
    36541 gttttgctca ttgcgaaaaa ggaaatcaat gtatcagcac atgcagaaac aactatacct
    36601 atagaagtga aaatcccagc aaaaagcttt aagggacgtg tattagcggg tattcatgtt
    36661 tcacctaaag aagaagctga aacagaaaat gctacagaag gagcacaaat caaaaatcgt
    36721 attgcttata atctcgcagt cgttttacaa gaaagtcaag agacgataga accggatctg
    36781 aaacttttat caggagattt ggacgaggtc aacgcaaagc caactgttca gcttcgtttc
    36841 caaaatcctc agcctaggat tatcagcaat ttgatattta cgagcaagat cttttatgaa
    36901 aatcagcttt atattgaaaa tacgtctaat gaatttttag ttgcaccaaa ttcgaatttt
    36961 cacttgaatc ttgatttagc tggtgataaa gcaaaagccg gcgattaccg cgcagaaatc
    37021 atagcaaaat caggcgacag taatgaatgg cgatttacac aaaactttac gatcaaaaaa
    37081 gaaaaagcac aaaaagtaaa tgaaaattcc gtttttgcag tacaagaaca atctttccca
    37141 tggctttatg caggattggt tgctgcagtt ctagtgttat ggttgatttt gctgtttttg
    37201 tgggtccaca agaaaaagaa aaaaggagaa acaacatgat gaggtgattt cgttgaaaac
    37261 atcgatctta ttgggaggga ttattgcaag tttgctgttc ttcagctggc aagagccagt
    37321 ccaagcagat accaaaagtt caggtacgat tacattttat gatctaagtc cagttccgca
    37381 tccaagtggt tcagtcaatg gcgcatcaaa acagcagaca cagctaccga tttataaaga
    37441 atctaatcaa catggcgtat tcccggagac gaatgaagaa aagatccgtt ttctttattt
    37501 gatcattggt atagaattat ggcttcttgc gtggttgttg ttatggagaa gaaaggaaaa
    37561 ggaggatgaa catgttgcta caaaataaat tttcattttt tcgctccttc ttttttactt
    37621 cgctttttgt tttaagtata tttatctttt caccttcggc agaagctgtt ggagcaacgc
    37681 ctcctgccat ttcttatgat ccatacacgt atgaaaatat cgtacctcca aatggtggac
    37741 gtagtgcagt caatggcggg aagcttgtcc aactaactgg ggctaaccaa ttgcctaaat
    37801 tgactgctac ttatttgaac gatggttcaa atgatccttg gaaaggtggc acggtccgtt
    37861 ttcattggga aagccatgtg aaactaacaa atgtacttga tttggtgaat atcactgcac
    37921 aaggatttgc atttgtggcg atcatcaatt tgccggaaca tgtatcaagc agtgatgtat
    37981 taaaagcaat tgactggaac aacgcctact tgtatattgc cggtgccccc atcaaggtta
    38041 ctagcggtag tttattatca gcaggaagcc ataccttgcg ttttgggatg gggtcgtggg
    38101 atacaaatac tctattggca gatattattg ctattattac gcgaggcgaa ttcaacgtgg
    38161 acgatatccc gcttcaattt gatgttacag ctaatgtcgc gaagatgaca gaaaacggag
    38221 ataaatatga tacgagtcca aacaaaattt tgaccaaggg taaattacaa ccttctatgt
    38281 ctaaaaaggc cgttttttct gttgattttt atgattcgaa taatattatc caagatcgta
    38341 tgttcaattc gattttagct ccagctgcag gtagatttta tcctaaatta actggcggaa
    38401 acttgaatcc aggagcatat gcattttctg caagtgcaga gataaatacg tggaatagtt
    38461 acatctcaac aagtgattca acaggaaaat ataatacatt tgctgatacc acagaatacg
    38521 tggatggaaa taaccagaac cttccaggct ctaccaatag tcggacattg acgatggggc
    38581 tgactgaagg acgttttgat cagttgccat ttagtcgctt tgatcgcgta gtcaactatt
    38641 ttacaggaaa aaacgttaca caaggagcaa tactgactca tactccagca agcacaagtg
    38701 gattgaatca gacagccaat atcgtgtaca gtgggaaaga tgcgcaaggc ggattgcttt
    38761 ctcctgttgc tttaaaggta ttggagcagt acaagctaga cggaacggtt ttaccaacaa
    38821 acttaacagc cgatagtcag tggggaaagc tcctttcttc tgcatcgtac caagtcacgg
    38881 ccaattggtc agcaactgaa ctaaaaaagg gttcgatcca ttacaggctt tattcgaaag
    38941 gaacccaaaa attagcgaca ggatggacag atcaactttt ccaaacgatc caatcaaata
    39001 acgggggtgt aaatacagct acttctacac tacctgcatt ggcttcaggg caatattatt
    39061 ttgattatcg cttggtggac gatgtactta ctaacatgta tccgtctttt gcgtacaaat
    39121 ggcagtctga acaagctgga atcaccactc ttcctcaaat aacagtggct aattttccat
    39181 ctgtgagtgc agatagtcag ctaaaaaacc ttagcagaca acctgagaca ggagatcccc
    39241 tgtctgctct atcaggagat acgattcaag aaaatgtgac attcacgtta acaaaatctg
    39301 gggatgcgat aaccgacaaa aaaatcacga tctcacttcc tgaaaatact acttatatca
    39361 atggttcctt aaaattaaat ggtacaacta tcgcagatac cggcattcaa caaggagttt
    39421 ctgtcccagc agatctattg agcaaaatag gagataccat tcatcttact tacaactatc
    39481 aattaaatac tgtagatact tctgtacaat ccgtcagcat actgacaaag gcagctgtac
    39541 tttccagcaa tattacatta gcagatggtg ctaagcttcc taacccagtt gtccaaacat
    39601 ctgctaaaac aattcttgtc cccaaacaag aattaacatt agttaatgta ccagatgatt
    39661 ttacatttgg taacgatctc cccaaaccgt tgaagacaag ttattatgaa gcaaaaggcg
    39721 atttttcttt tgatgtgcgg gatactcggc ttccaagcac gtctccttgg caactgacag
    39781 ggacattgac ttctttattt aagaatgatc aaggtcaaga attaagcgga acaaaattat
    39841 acttcaatca ttctggatca aaacaattga ttcagcaagg tcaaaacacg ttgatttatg
    39901 agagtgatgg aacagctaaa ggagaggttc tcgttgactt tcccgatacc gatggtttac
    39961 tcttagaggt aaatagcagt acgaatgctc aacctggcgc aacgtatcaa ggcatggtca
    40021 catgggaact gacagctggg ccaaccagct aagtgattag aatttgtatg gatctgtgat
    40081 aactgtcaca gatcctcttt ttcaaggatg agaaggaaac ttctgctgtt aaaatcagaa
    40141 atgcttggag gagtggactt ggaacttttc agtttgcttg aaaaaaatga acaacggcaa
    40201 atcaaacttt tccaactttt attagaggaa aaaggagaga tttccgttaa taacgtactt
    40261 acccgcttat cagttgatcg ggctaccttg aaagaagatc tgttttattt acaagctaac
    40321 ttggaaaata tcaaacaatt ggaaattgtt atccaagaag ggaatatttt attaatcaga
    40381 catggaaata tagcagcagt cgatgtttat tatcattgct acttgcgcta ttctacaaaa
    40441 tatcagatac ttctttatct gttggaaaat ggctcatttg atcggcagaa acttgtcatg
    40501 gaacttaata tcagttcggc tactttgtct cgcagagtga aggaattaaa tgattcttta
    40561 aatgagtttc atctgaaaat aaaaagcggg cggctgaccg gtccagaaag tcaaatttgt
    40621 tatttttatt tccaattatt ttggtttgga cgtccttatc ttgaaaacaa aaaagaatac
    40681 agtgatttat caggaaacac ttttttagat ctgctgcaaa aagagtttag ttttactatt
    40741 acagaagaag gacgattgaa atttgaaata tggctttcca ttatgaagaa gcgaatgcgc
    40801 tgtacgaaaa aatccaaacc agattatagc aatcttcctg ttggattgtt tcaaacgaca
    40861 caatttttat tggacctcca tacatttttg acccgttact tctttcaata tgcctttcct
    40921 tggaatcaaa atgaaacgtt ggttttttat cttttcatga tcagtaactt tacattagat
    40981 acacataact cgcatgtcca ggagtttttg ataagcgaaa aatacaaaga cgacgtagta
    41041 gctcaattga atcgaacgtt taaacaatcg atagaagaat atttctcttt ttatgttttt
    41101 ccagaagaga tttcgcaaaa gattgcgttg acagtgatgc aaaatcacgc tcattttgcc
    41161 tattttagag gatggatgaa tatttttggc caagaaggat taaaacagcg gttaactgaa
    41221 gtagaagata aacaattcct ggttttatgt gaaagattgg tcagacaaac atttgctatt
    41281 gttgatttta ctgaagtaga cgaggaaaat gcccgctttg aattatttgc tcgctatgca
    41341 tctattttgc ggacgctgat caagcagtta gaattacgtc tgcaaattgc ttatgatctt
    41401 ccttatgaaa ggctaatggc ggatatcgtg aaagaaatca tccaggaaca attgctgaat
    41461 acgatcaaat atgatttagt cgaatacgag aaagaaaagc agtatgatat cattttgact
    41521 agccaattga aagagtaccc gtcacagaag tccgctaaag tttttgtatt cactagcaac
    41581 gaatataatt atgatttccc ctatttgaac caatttttaa aagaatgtta tttagaaaaa
    41641 ttgaatttaa ggatgaataa aacatgaaaa tcgtgatcat tggtgcttcc catgcaggca
    41701 tcacagctgc cctaaatcta agaaaacttc aaccgaaagc agaagtttta ctgatcgatg
    41761 aagatcataa agatggacta ggctatgttt caaatggaat caacctttat ttaaaaggaa
    41821 agatacgtag tttagctgaa gttgcccata acatgcggac tcttcaagaa agtggggcaa
    41881 aactgatcac cgaatggaga gtaactgaac ttgatccaga caaacatcag ctcatcctcg
    41941 catccaaaga aggaaaaaac gagaccataa cctatgataa attaatcgta gcaacaggtt
    42001 cctctcctgt caccttgtat aaacaaattg aagctgaaaa tgtctacacc tataaaaatc
    42061 tagtgcaatc taagcaagta cttgccgctt taaaagaagc aaaagaagta gtgatcttcg
    42121 gtgctggtta tattggattg gaattagcag atgctttgag aaataaagga tacattattc
    42181 atctagttga ttatatgccg aatgttttat caagatattt cgataaggat atgatcaaca
    42241 gttttcaaaa tcagctgcaa actaagcaaa tcaattttta tccaaatgaa ttcttgatcg
    42301 attggaaaaa atcagaagag aaagtagtat ccgtacaact tctatctcaa gcaataaagg
    42361 cagatatggt cattttttct gcccaaactc gtcctaatac gacattgtta aaagaaaaag
    42421 tggctttgta tgaggatgag actgtgatag tcaatgaata tctgcagaca agtgatccgg
    42481 acatctatgc gattggagat atcgtcccag tcagttttga caaaaataaa cggcatctct
    42541 ttttaccttt agttactcga gctgtacata tggcaagagc agtagcgcta actttgagtg
    42601 gtcagccaac cgcttacgac ctcagacaaa agataacagc agcagtcatt atagactact
    42661 ttttaggaac tgtagggctg acagaagatg aagcgccatt cttggaacaa aataccggtt
    42721 cgtgttcagg agaatttgat ctatttccac aatacgatga agaaaacaaa acagtaaatg
    42781 caaagctgat ctaccatccg gatacattag aaataattgg cggacagctg ataagccaag
    42841 aattcttatt atcagatttg aacctgcttg cggatatcgt gaaacacaaa actacgattc
    42901 cacagctagc agttgaggat tttggatttc tgtctgaaca cacctctcgt tttcactacc
    42961 tcaatgagtt agcttttaaa gtactagcta agagagcaaa tagcaataga gtagataaaa
    43021 gactgtgaga aatatcacgg tcttttattg tgtggggata taataaatat taatcaatcg
    43081 agaataaaaa attcatataa tgaaatagcg gaagaatttt ttgagcataa tattcgccaa
    43141 aaatttatca agtgataaaa ttgttgtata aaatgattat ttcaggagga ggagttaaat
    43201 gtttttagca ttaaatgaaa ttatgcattc aaagttacgt tatgcgttag ttgcaggggt
    43261 gatgtttttg atcgcctacc tggtattttt tttgactggg ttagcttatg gcttagctca
    43321 agataatcga acagcagtcg acaagtggga ggcagattcg attgtgcttt cgaaagatgc
    43381 aaattcaaat ctagggatgt ccatgattac taagaagata gcagaagaag tcgaaggtgg
    43441 gaaagtggcg tatcttgccc agacacctgg agtcgtgact agtaaagaca gtacagaaga
    43501 aggaaaaatc aatgttagct tcttcggtat cgataaaaat caattcatca tgcccaatct
    43561 tgttgaagga aaagcttttg ataacgatga tgaggcagta ggcgatatta gtttgaaaga
    43621 agaatatggt ctagccgtag gagatacagt taagttatct ggtagtgaca agacgtttaa
    43681 actaactggc tttacggatc atgccaaatt caatgtctca ccagttctct atacaaccat
    43741 caatgcttac caagagatcc gcttcgaaaa agaagataca agtgaaaatg cacggattaa
    43801 cgcaatagtt gtcagaggta aaatcagtaa tttacctgaa gatttagaac agatcaaaat
    43861 ttctaaattc atcaatgaat tacctggata taatgcgcaa gtattgactt ttggatttat
    43921 gatcggattt ttgatcgtta ttgcggctat tgtgatcggg attttcattt atgtactgac
    43981 gatgcaaaag attaatatct ttggtgtaat gaaagcacaa gggatcacag gaggatttat
    44041 tgcgcgttct gtagtggcac aaacattcat tttatcattt gttggtattc ttcttggtct
    44101 attaggaaca gtgggaacat cccttgttct acctgatgcg gttccattcc aaagcaattg
    44161 gctgtttttt ggcgtcatta gcttgttgat gctggtagta gctgtgttag gcgcactatt
    44221 ttctgttcgg acaatcgtaa aaatcgatcc gttgaaggca attggataag aaggaggaag
    44281 agcatgaaag caatagaatt taaagaagta gaaaaaagtt tccaagatgg cgaccaagta
    44341 gttaaagcat tgaaagaaac aaccatgagt atcgataaag gggaatttgt tgcaatcatc
    44401 ggtccttctg gttcaggtaa aagtactttt ttgactttgg caggcggatt gcagacgcct
    44461 agcagtggct ctgtatttat caatgatcaa gaattcagta atcaaaaaga aagtgaacgc
    44521 gttaaactac gttttaaaga aattggcttt attttgcaag cttccaatct ggttcctttt
    44581 ctgacagtaa aaaagcagtt attattagta gataagatca aacatgaaca tcgtcaaaaa
    44641 gaagcaaaag aattatttga gcagcttggc gtggataagt tgatcaacaa gtatccagaa
    44701 gaactatctg gtggagaacg gcaaagggtc gcaatcgctc gtgcattgta caatgatccg
    44761 tccattattc tagcagatga accaactgct agtttggatt cagaaaaggc acgagaagta
    44821 gtcgatatct tggcaaaaga atcaaaagac aaaaataaag caacgatcat ggttactcac
    44881 gatacacggc tgattgacaa atgcgataga atatttatga tggaagatgg tgtattgaaa
    44941 gaaaaagaat aaccaaaaag caggtggaaa aatggtccat cgatacaaca aagatacaca
    45001 gcaaaaaatt ttgaaagaag cgactgactt gtttatgtcc aagggatatc tcggcacttc
    45061 aacaagagag atcgcacaaa aagttgggat cacccaaccc aatctctatc attatttcgg
    45121 tgataaagaa aaactatata cggcagtttt agaaaaccac ttaaaagatg ttggaaaagc
    45181 tttgagagaa atcgttcaaa caagcgaaac gggatttcaa gtaaccttga caaaaatggc
    45241 gcagtttttg attgagacac atctggtcga tttgtttctg atgcttcacg atcttgaaag
    45301 caatctttca aaggaaacaa gagatcatct tttctttcta tggaagaaaa attatcgtga
    45361 accttttgaa gaaatctttt cagcaaacca atctgtgcta agaaatggga tttcaaaaga
    45421 aatcgcagca aggcattttt tcttagtgtt agcaccttac atcactcggt cgaaaagacc
    45481 tgtggaagaa ccgctaaatg tggcgcaatt gatcgattta tatttgaatg gtgtattagg
    45541 agaagcgtaa agctgttata tagggagaaa aatagttagg tgaagaagca agatatgacg
    45601 aagatcgtta tatcttgttt tttttatata ggtagttttt cataaataca aatttgttat
    45661 tctctcatga ttatcagggt atatttttat ttattatgtc ggtgttccag gaagtgtttt
    45721 aatttaataa cgaagaaatt aattgtttgt agaatgaagg aaactattta ttgggtagat
    45781 gaatcaccac gaatagtcaa aaggagattt caggatatgc caaaaatgaa aaaaatcatt
    45841 ttagcagtaa tgttattttc tacggttata tcttttacag cccctttttt gcgtacttaa
    45901 gctgatttta agttttgacc cttagtctgg atctacgagg tgagagtcaa gaatgaaaaa
    45961 aataattgga gcttttgtat ttgcaatcat tatcattatt atctgttttc aaatagatgt
    46021 ttttggtcaa aaaaagatag ttggacaaac ggtcagcagt acgaacagta cggttacaaa
    46081 gacaaaggaa tcatttgatg atacagaaca agagacatta atcaaaatcg gaaaaaatga
    46141 ccgtacacta tatacagatg actcgctaac taccccactt gcaactataa acggcggaga
    46201 gctggcggag tttctttctg aaacaaaaaa tgcttatcag atcaagacaa atgatggcta
    46261 tactggatat ttagccctcg aagatggaac caaagtcaca aagaatatac aaacaaaacc
    46321 ggaagagcta tctgatgcag tgattgtatt agatcctggg catggcggaa atgatactgg
    46381 ggcattaagc aatgatgaac aaacagaaga aaaagatata acattaagta cagccataaa
    46441 agtaaaaaag gcgctagagg atgctggagc aacggtttat ttgacccata ctacagatga
    46501 attagtacag cttggagata tatgcgacta cagtgaagaa aaaaaggcag atgtctttat
    46561 cagtttacac gcagactcta cggaatatgc gaatgaagca actggaatca cgacatatta
    46621 ttactatgga caagaggaaa cattagcaca gacaattgct gactccttca ctgatctgcc
    46681 tctggattca agaggaatct ccactgggaa ctatcaagtt cttcgagaaa atcttcaacc
    46741 ttctatttta atcgagatgg gttatatgaa taatgatagt gatttaaagg aattagtaac
    46801 agacagttat cagcagcaga ttgctgcaag tttgacacaa gcattgacca cttattttca
    46861 gtaataaata agcccagcca aacaaaaata gctcctcata tttttcgttt ggccgggctt
    46921 attatcgcag atgcaagctt ttgaacaccg tttaaggaag aggttgtgag cctgccgttt
    46981 aactccgcgt cacaaggagc aatttcaaaa aacagcttct catgtttgat gactattgtg
    47041 acttgtgacc gaggagttgg catgagaaca ccgtttattc ggaacaaggg actgtgacat
    47101 gacttttgtc acagttccat tattttttta gcagcttcaa agctttttct gtattggcaa
    47161 aatgaagttt ggcatatttt tcaagcatag gcatgccgcc tttctccaat agttttgccg
    47221 ctacttggtc agatttcgtt ccagctcctg atggaagggt gattccagct tcagctgatg
    47281 ctttgtgtaa ctcttccaaa aagctggcac gactgatgat agaagcagca gcaacagcta
    47341 aatgatattg ttctccttta gtaacgaagt acagtttttc ttgtaattga tttttttcta
    47401 aacgcgcata ttttttaaaa tttgcttccg gcgtaaactg atcgatcaaa atcgcttctg
    47461 gtttttctgg ctcgattttt tttagcagca aatggatcgc ttgattgtgc aaagcgactt
    47521 tcatccgaac ggcattgtat tttggttgga tctcattgta tttttctgga gataagacaa
    47581 gcagtttata ggggatggtt ttttttaaga tgtcagcaag ctggatgatt tggctatctt
    47641 ttaactcttt tgaatctcta acaccaagtt tacgtaattc tgcaagctga tcacgtttca
    47701 cataagcagc acatactgtt agcggaccaa aatagctgcc atttccgacc tcatcactac
    47761 ctaaaacgga taattgactg atattttttg gcaaagaaga ggctgttttt gctggcttgg
    47821 cttgtttatc ctgtgcctcc ccccatttat cagcttctat tgctgcattc gtaccttgaa
    47881 acatcacttt tccggatgta taagcggtaa tggtcgtccc gtcttttttt gcagcaaagc
    47941 tagtataagg aatctgttta tttaacaaat aagagcgata gtatgctttc atttgactaa
    48001 gttggtcgtt tgttgctttg ataatgacgt tgctcataaa attctcctcg tttttataca
    48061 taaaactata ccgcaactca cgacttttcg ttttctaaga gattgcgaga catagcattt
    48121 ttttaaagtt tatcatataa tagttaagta tgtgaaaaat atttgtggag aacggtgaaa
    48181 gaaccaactt catggtacac taaataaaac tttcatcaaa taggaggaaa tgattcgtat
    48241 ggcgcacgaa aaaacacgct acaaagcagt gattgccaac caaacatata ctattatcgg
    48301 acgggaaacc aagcatcata tggatatcgt aacgaagctg atcaatgaac agttagcaga
    48361 attgaaacaa ttatcaccac aaatggataa tgaacaagca gcaatcttga tggctgtcaa
    48421 tgcgctttcg gatcaattga agaaacaaga aagaatcttg gagctggaag aagaaacagc
    48481 agaattaaag aaaaaaatga tcaaatttac agagcttgaa aatcgtgtga aacgcattga
    48541 agcaatcgag aacgaagctc gggaagtatt gaaagaaaat ggtcaagcag atcataccat
    48601 taaaaatcat gtggaagcac aacagatttt aaacgagaaa cgaaaaggac aaattaaaca
    48661 aaaatcttct aattaagatc cttttatcaa aataagaaaa atttaaaaaa gttagaagag
    48721 aaaggaacgt agcatgctga gtttgctgat tttattttta ttgctaattg cgtttttttc
    48781 tggagcacgt cgcgggtttg ctttgcaaat catattcgcg attggatatg tattgtcatt
    48841 tattgctgcc cagcattttt acaaaccatt agcaagtcat ttggaattat acattccata
    48901 cccagctgtc accccaacat caaagttagc attttttgat caggtatttg cttttcacct
    48961 agatgaagct ttttatgcag gaactgcttt tctgattatt ttgctaattg gctggctgct
    49021 tacccgattt gtgggggttt ttgtccatgg attgacgtat gtgccgatct tgagacaggt
    49081 agactggatt gcaggaggca tcctgtcact aatcatggct tacgttacta tatttttgat
    49141 tttgcagtta cttgcctttg ttccattaga tgtggtccaa aatcaattcg gcgagcatag
    49201 tttcgctcgt tttatcgttg agagaacgcc atttcttacc aacaagatgt atgagctttg
    49261 ggtaacgaat attttgaatt aaggaaaata gtgctggaaa atatttcgtt ttttgtacag
    49321 agtagtttat ttaagaaggc tgcgtagcgt atgtccggca cagctctttt ttcatagtga
    49381 aagataaggt gataaaatga ataaccgaat ccttgaaaca ttggaatttg aaaaagtcaa
    49441 acagatggtc agacaatttg tagtgactgc acaaggaaaa gaagaactgg cagaattggt
    49501 accagtgagt gagaaacaaa cgatcacaaa ctggcttcaa gaaactgagg atggattgaa
    49561 agtgcaacgt ctgcgaggcg ggatccctat acctaaactt gaaaatatcc gtccccatat
    49621 gaaacggatc gagatcggag cagacttgaa tggagtggag cttgcacaag tttccagagt
    49681 attgtctacg acaagcgaat tgaatcgttt cattgatgat ctttctgaca gtgaaatcga
    49741 atttgcacgt ttgtatatgt gggcagatca gttagtcacg atccctgttt tgagccgccg
    49801 tttaaaggaa gcaatcgatg aagacggacg cgtgactgat gacgcttctc ctgagttaaa
    49861 gagcataaga cagaacattc ggcgaagtga acaagctgtt cgtgaacagt tagatgggat
    49921 cgtccgtggg aaaaatgcaa agtatttaag tgatgcgatc atcaccatgc gaaatgaccg
    49981 ctacgttatc ccagtcaaac aagaataccg cggtgtattt ggcggtgtag tccacgatca
    50041 aagtgcttca ggacagactt tatttattga accaaaacaa gtggtggatt tgaataatcg
    50101 tttgcgtcaa taccaaatcg ctgaacgaaa tgagattcaa cgtattttaa gtgaactttc
    50161 tgctgaactt gttccccatc gtcaagaaat catccacaat gcttatgtga ttggtaaaat
    50221 ggatctgatg aatgcaaaag cacgatttgg taaagaagta aaagccatcg tgccgggaat
    50281 cagtgaagac aatcatgtcg tcttgaaaca agcaagacat ccactgatcg atcaggaaaa
    50341 agtagtttct aatgacatca caatcggaaa agactatcaa gccatcgtca ttacaggacc
    50401 taacacaggt gggaaaacga tcacgttgaa gactttaggt ctgttacaac tgatggggca
    50461 agcaggatta ccgattcctg caggtgaaga aagtcaaatc ggtatttttg aggaagtatt
    50521 tgcggatata ggagacgaac agtcgattga gcaaagtcta tcgacctttt cttctcatat
    50581 gacgaacact gtagatattt tatcaaaagt caatgaaaaa agtctggtat tatttgacga
    50641 gctcggtgca ggaacagatc cgcaagaagg tgctgctcta gcaatcgcaa tcttagatga
    50701 tcttggtaaa aagtctgctt atgtgatggc aacaacgcat tatcctgaat tgaaagttta
    50761 tggatacaat cgggcaaata cgattaatgc cagcatggag ttcgatgtgg atacactaag
    50821 tccaacgtat cgtttgctga ttggcgttcc tggacgaagc aatgcgtttg aaatctctag
    50881 ccgattaggt cttgatacag aagttattga tgaagcgaag cagttgatga atgatgagag
    50941 ccaagattta aacgaaatga tcacagattt agagaatcgc cggaaaatgg cagagaccga
    51001 atatctggaa atgcgtcatt ttgtttctga ggcacaagaa cttcacgatg atttgaaaga
    51061 agcttacagc tatttctttg aagaacgtga aaaagaaatg gaaaaagcaa agaaaaaagc
    51121 aaacgaagtc gtgtcagagg cggaagaaaa agcagaaaaa atcattgctg atattcgtaa
    51181 aatgcaacag cagattgggc aaggaaatgt gaaagagcat cagttgatcg atgcaaagac
    51241 ccaattagca aatctacatc aagaagaaac tttgaagaaa aataaagtat tgaagaaggc
    51301 gaaagaacaa aaaacgttga aaccgggaga cgaagtgttg gttacgactt acggccaaag
    51361 agggacgctt ttacgtaaaa acgggaatca gtggcaagta gaaatcggta tattaaaaat
    51421 gaatgtgtcc gaagacgagc tgacgcctgt tgctcctcaa aaagagccga cacaacgagt
    51481 tatccatgct gtacgatctg aatctagtag tcacgtgccg aatcaattgg accttcgagg
    51541 aaaacgatac gaagaagcgc taagtgaggt agatcaatat ttggattctg ccattctagc
    51601 tggttatcct caagtgacta ttgtccacgg aaaaggaaca ggcgcacttc gcaagggaat
    51661 tactgattat cttaaaaacc atcggagcgt caaaagcttt gagtttgcac cggcaaacca
    51721 aggcggaaat ggtgcgacaa ttgttaaatt taagtaaagc ttactaaagg taagcagatg
    51781 gttcgctatc caagtataga aatgctatac taatcataga ttttaaaaat ggaggtcttt
    51841 gatatgtcac aagtaattac agataagaca tttaatgaag aaacagataa aggacttgta
    51901 ttgattgatt tttgggcaac ttggtgtggt ccatgtcgta tgcaagcacc gattttggat
    51961 cagttggaac aagaatatga tgaagaagaa ttcagaattg ctaaaatgga tgtggatgaa
    52021 aatccagaaa ctcctcaaca attcggtatc atgagcattc caacattaat gttgaaaaaa
    52081 gatggtcaag tagtagaaaa agctgttggt gttcattcaa aagaacaact acgtcaaatg
    52141 atcgatcaat atttataaaa acttacagca aaaataagct gagggtggga ctgaatccag
    52201 ttccgtcctc ggtttttttg cggttgagtt aaggttattc aaattaatat tttttgagta
    52261 taattacgtt gtcaggctgg attgtttttt tgacagacat gctataataa ctttaattga
    52321 atatgaaggg gagtaccaga tgaacgaacg aatcaaaaac aaattagctt tacttcctga
    52381 tcagcccggc tgttacttga tgaaagataa aaatggaacg atcatttatg tcggcaaagc
    52441 caaaatatta aaaaatcgtg tccgctcata ttttactggc agccataata cgaaaacgga
    52501 acggcttgtt agtgaaatcg aagactttga atacatcgtt acagaatcga atatcgaagc
    52561 attgctctta gagatcaatc tgataaagaa aaatgatcct aaatacaaca ttatgttaaa
    52621 ggatgataaa acctatcctt tccttaagat aacgaatgaa aaatatcccc gtttggtgat
    52681 tactcgtaaa gtactaaaag acaaagcact gtattttgga ccatatcccg atgtcggtgc
    52741 ggcgaatgag acaaagaaga tcctcgatcg attatttcct ctgagaaaat gcaaacctag
    52801 tcagacgaag gagccttgtc tttactatca tttgggacaa tgtctgtgtc cttattattt
    52861 cgacgtagat ccgaaagtct atacgaaaat cgttgatgaa gtgaaaaagt ttttaaacgg
    52921 aggacacgaa aaaatcgagt cagagatcca tcagaaaatg gaaaaagctg ctgaaaacat
    52981 ggagtttgaa aaagctgcag aataccgaga ccagatccgt gccattgaga caatcatgac
    53041 tcgccaaaaa atgacaaata ctgatttact ggatcgagat gtatttggtt atgcgattga
    53101 caaaggatgg atgtgtgtcc aagtgttttt cgtaagacaa ggaaagctaa ttgaacgaga
    53161 tgtttcaatg tttcctttct ataacgaagc agaagaagat ttcttgactt acctcggtca
    53221 gttttatcag gaaaatgagc atttcattcc taaagaagtc ttgattcctg atacgatcga
    53281 caagcccagt gtagaagctt tgttagctac gaaagtgctg caaccaaaac gaggagaaaa
    53341 gaaaaaatta gttgaattgg cgaataagaa tgcctcagtt gctttgaatg aacggtttga
    53401 tctgattgcc agaaaacagg aacggacgat cggagcagtt gaacgtttag gaaacgcgat
    53461 gaatatcccg accccaatac gtatcgagtc ttttgataac tctaatatca tggggacgaa
    53521 tcctgtggct gcgatggtcg tattcattga tggaaaaccg gcaagaaaag aatatcggaa
    53581 atataaaata aaaacagtca acggaccgga tgattatgca tcaatgagag aagtcatcta
    53641 tcgcagatat tcacgagtga ttcgcgaagg gttgccttta cctgatttga tcttgatcga
    53701 tggaggaaaa ggacaagtag atgtggcaaa agatgtctta gaaaatcaat taggaatcga
    53761 tattcctatt gctggcatgg caaaaaacga taaacataaa acaaatgagt tgctattcgg
    53821 tatagatttg gctgtagtac cgcttgagcg aaattcacca gagtttttct tactccagcg
    53881 aatccaagat gaagtccatc gattcgcaat cactttccat cggcagctac gcagtaaaaa
    53941 cagttttgct tcccgattag atgaaattga agggttagga ccaaaacgaa aaaaaatgct
    54001 gcttaaagag ttcaaatcgt tgaaaaatat cacagcagcc acgacagaag aattacaaga
    54061 aatcggactg ccgaaaaatg tcgcacaaaa cgtatttgat aaattacatc aggaatgagg
    54121 aaagggcact ttacaaagaa agtaaagtgt cctatcattt tatgtacaac aaaaagagcg
    54181 tttacgtttt gttggtaaat aataaatcgc ttacgtctgg tgtctaagga tatatgtgat
    54241 ttcttgaaga aggagtgaag aaatgttaga agtcaaggat cttgttaaaa cttttgggaa
    54301 ctacacagct gtcgatcatg tctctttcca aatccccgat ggaaaaatca tggggctaat
    54361 tggccaaaac ggggctggga agacaacgac ttttcgtttg atcttggatt ttttgacaca
    54421 agaccaagga gaagtgttat ggaatggcca tcatcttggg gagaaagatt atgatgtcat
    54481 cggttatctt ccggaggaac ggggacttta tccgaaagta acgattcaag accaactgct
    54541 ttattttgca gaacttagag ggaaatcaag aaaagagatt gaaccaaaaa tcgatttttg
    54601 gatggaaaaa tttcaggtaa aagggaaaaa gacggataaa gtaaaatctt tgtctaaagg
    54661 aaatcagcaa aaagtccagc tgatcgccac gttaatccat gaacctaaat tgatcatttt
    54721 ggatgagcca ttcagcgggt tagatccggt aaatgctgaa ttgctaaaag atggaatatt
    54781 ggaattgaaa aaacatggtt catgtgtcat tttttctagc cacaatatgg ataatgtaga
    54841 aaaaatctgc gatcatttga tcatgttgcg aaacggaaaa atggttttga atggaaaagt
    54901 acatgagatt cgtgaatcat ttggccgtac gaagttattc ttggaatctg gtttaagcca
    54961 acaggaagta gaagaaatcg aaggcgtgaa aaaagtgatc cttcatgaag acaatacgtt
    55021 agagattacc ttagaagatc ctgaagctgg gaaagaaatc tttactcgtg cgactcagtt
    55081 cggctacatt cctatgttta accagcaacc gccaactttg gaagagattt tcaaattgaa
    55141 agcaggtgaa atcgatgcgt aagttttgga ttattaccaa agatgtctat ttaaaaaatg
    55201 ttaagtcgat ttctttcttg atcatgatat tagtcccttt tatcatgctg ggtatcattt
    55261 atgtagcagg aaactttgct caacaaaata gtgatgtcaa taaaatcggc gtattggctg
    55321 ataatgagca aatcagtcag caaatgagcc aagtcaaaac agatgatttt gagttcaaag
    55381 cagttgattc tgaagaaaaa gcacaaaaag agctttcgga agaaaaaatc gatgcgtatt
    55441 tagtagtctc aactgagaat gataagataa atgggatgct ttatagcgaa aattcattag
    55501 gacaaagtac acaactactg atacaacaac aactgactgg cttgcaatcg atgcttcgag
    55561 caagtcaatt aggcatttca ccagaagaag tagctagctt gagccaacaa gcaagtttga
    55621 ctcgacaaaa agtcaatttt gattcaaaag gaaaaatgac agttggagaa gataactcga
    55681 cagtccaata tgtcgtaagt tatgtagcaa cgattgtctt gttcattatt attttgacgt
    55741 atgcgcagat cattgcacaa gagatcgctt ctgaaaaagg cacgcggatc atggaagtga
    55801 tcctgtccag tacgactgca caaaagcatt tttacggaaa actaacaggt gttttacttg
    55861 ttgcattaac gcaaatggca ttatacggta ttatttttgg tataggatac agtcaattca
    55921 aagatatgga aatggtcaaa aatatattag acggtatctc attggataac ttgattggac
    55981 cattcttgtg gtattctctt ctattcatga tctttggcat tttgattttt tctgttttag
    56041 cagctttatg tggctctttg gtcaacaaag cagaagatac agcaaaagca attttgccag
    56101 tgacttatct atctctaggc ggatatatgc ttggattgat actaggagct tcagatccaa
    56161 acaatatcgt tattcggatc acttcgtata ttccattctt atcttcatat attatgccgg
    56221 ttcgcttagc aaatgagaca gtcggtatgg gtggagcatt gatttccttg ttggtacttg
    56281 tagccgtaac attcttattg atgatcttgt ctgcaaacat gtacaaatcg aatgtactcg
    56341 tgtatagcga aggcggatta tggagttcat tgaaacaatc gatttcaatt atgagaaatg
    56401 aacgaaaaaa agcataaaat atagaaaaat atagaaggaa gctggacatt tattgttccc
    56461 agcttctttt ttgtatagac ctatttctat ttttttatta tattggatat atatattcat
    56521 tgaaaataga gcataacaac tttataatgt tgagtatacc aatttttgga ggcgttttaa
    56581 tggaccgcta tttagattta ctttcagaga agttccctac aacggaagat gtcatcacgg
    56641 aaatcatcaa tctcgaagca attttgcagc ttccaaaagg aacagagtta tttttgagcg
    56701 atatacatgg cgaatttcct gcttttgatc atattctgcg tatcggttca ggaaatctta
    56761 aagaaaagat caaagacctt ttctctgaac aaatgacgga aacggagatc agccaattca
    56821 ctatttttac ggcttatcca gaatatgtgt tgacgactga ttggtacaaa aaacaagata
    56881 aatcccagct gatccatcgt ttgatcgatc ttttacggtt tacgacagtc aaatacactc
    56941 gatcaaaagt tcgaaaagga ttgccgaaag aatatagcta tatcattgag gaactcttgt
    57001 atatcgatga tcgtatcaac gggaaaaaag actatgtaaa aaaaatcatt gaacaacttg
    57061 tcttgatgaa ggaagaagaa aaatttttaa caaaacttgc gcagacgatc caaaaatctg
    57121 tcatcgatca tttgcatatc gtgggtgata tttttgatcg aggaactcaa gcagccaaag
    57181 taatggatcg aatcatggat ttttcctctg tagatatcca gtgggggaat catgatattt
    57241 tgtggattgg tgcgtattgc ggctctgagg catgtttatt gacgctttta cggattgccg
    57301 cacgctacaa ctatttattt gaattagaga aagaatatgg attaaattta cgtccattat
    57361 tttcttttgc ccatcaaacc tatcagaaga atccagtatt tacccctaaa aacaggaaag
    57421 atctatctga aagagagcaa gaagaattag aaaagatcca tcaagcactt tctatcctcc
    57481 aattcaaatc agaacatcag ttgttgaatc gacgacctga atttaaaatg gacgatcgta
    57541 aattgttgga aaaaatcaac tatgaggaat caacgatcgt tttagaaggt cagctctatg
    57601 agttgaaagg gacttgtttt caaacgatcc agccagatga tcctaaaaaa ttcttggaag
    57661 aggaacaaaa agtgatcgat agtttgatgt attcattcca gcactcgtta agaatgcaaa
    57721 aacacatgac gtttttgatc gaaaaagggg gaatgtatct cacgaacaat cagcatgtac
    57781 tgttccatgg ctgtatacct gtagatgaaa aggggcagct cttagcattt gaattagacc
    57841 aatcttatcg cggaaaagaa ctgctgggat tttttgaaaa acagataact gaaagcgcaa
    57901 aagatttgac tgcaaatgaa gatttggcga ccgatttgat ttggtatgcg tggagcgggc
    57961 cattttcgcc tttattcggc aagagtaaaa tggcaacttt tgagcgctat tttctcacag
    58021 agcctcatac gcacgtcgaa aaaagcaatc cctattatca cttgcgagat gaagagtggt
    58081 tcagtgaaaa aattttggca gaattcgatg caaaagaaga aggttcagca atcatcaatg
    58141 gacacacgcc agtaaaagta aaaaaaggag agtcaccgat caaagcaaac ggaaaagcat
    58201 ttgttattga cggaggtcta tccaaagctt atcaaaaaac aaccgggatt gctggctact
    58261 cattactatg caattcttac ggtttccaaa tcgtgacaca ttatccgttc ttgcctattg
    58321 aacaacaatt tgcaaaaaaa tctgatcaaa cgaatgtaaa aaaagtgatc gagcagcagc
    58381 tcccacgcaa acttaatcgg aatacgacaa aaggaatgga attacagcaa cagattactc
    58441 aactccaaag gctgttggat tatcgaaagg acgattgaga aaaatgctag ccaacattgc
    58501 tcaatagact aaaaagcgac tgcaacaaaa ggcgtgtcgc agtctccttc ggaattagct
    58561 caaaaaacgg taaaatatta gaagctattt ttctgtttat ttctctaata ctcaggtcag
    58621 ggcgctgatt tcacgacctc ttccttaaac ggtgttctcc tgccaactcc tcggtcacaa
    58681 gtcacaatat ccaacaaaca tgagaagctg ttttttgaaa ttgctccttg tgactcagag
    58741 ttgaacggca ggctcacgac ctaatcagaa aaacgattaa aattcattca caaagaaatc
    58801 gaataaacgc tgttcagatg aatctacacg atgagaaagt tccggatgcc attggacacc
    58861 aaggattttg tgcatattat ctttactttg gactgcttca atgagtccat cgttggaaaa
    58921 ggcgatggct tttaacaaag gagaaagatc tttgactgcc tgatgatgat aggaattcac
    58981 aaagtatgaa tcagggagaa ggtcacttaa taaactgtcc gaaacgattt tgacttcgtg
    59041 agttgcaaac tgcggggcag tgggctgttg ttcgtgtttt acggaccatt ttggatataa
    59101 tgaaagatct tgataaagtg ttccctccaa cacgacattc aatagttgaa gtcctcggca
    59161 gactgcaaaa atcggttttc tttgtttcaa tgcttccaag atcaatgcct gttcgaattg
    59221 gtctcgttga atatttgttt ccgtcagttt tggatgaggg tcttccatgt aaagttgcgg
    59281 actaacatcc tgaccgcctg caagaatgag tttgtcgatt ttctcaatta gttgaggcgc
    59341 tgaatctgga gacatgactg gcagaataag aggtaatcct tgagcatcta aaactgcatc
    59401 cacaaatccc tgcggtgtat agcttacttg atttccttgg aaagtatctg tcgctcgaat
    59461 cagttgattc ccggcaatgc cgatgattgg tttcattttt attcccccta aagtagacta
    59521 attgtaacaa ggatttaaaa aaatttaaac tatgtaagac ttgcccctat acttatctgt
    59581 tcttgctgtg ttactatctg taaagagaat tttcgtgtga aattcatgac gatagttgaa
    59641 aatattgctt aatttgttat aataaaaagt aagcagaaat aaaaaggttg ggcgtaagct
    59701 tcaaggatct agttcttcaa ggggtgagaa ggacatttta ttgaaggggc ctgcctatac
    59761 taagttatct ttagaggtac atactttcaa tgaaaaaaga aagcatgtac ctctttttta
    59821 aatcgaaagg aagtgaagca agtgttagaa gctttggaac aaatccgaaa agcagaagaa
    59881 gaaaatgaga agcgtgctcg aaccctacgg cagcagttga agacctatgc agaagaaaaa
    59941 gagcaagaat tggagataat ccaaacgaac agtcggcaac agctgaaaca gttggtagaa
    60001 gaaaaagaac aattcgaaaa gcaagaatta gaaaaagaaa aaatgaaatt gaaagcagaa
    60061 gcagacaagg tgcaagcaac tttgaaagac caatatcaaa agcatcagag tcaagcaatc
    60121 ggtgcaatca ttgaaagggt gaaagaaact tatggccgtc actaagatgg aaaaagtgac
    60181 cctcatttca gataaaagaa atcaggagag gattttacaa gcagtacaag gaatccaaaa
    60241 tgttgaaatc cgggatctgt atcaaaatag aacaaacaac cagtgggtag aaaaatattt
    60301 tccagatacc acgattattg ataaagaagc aaaattaagc gctttaggca atcggctgaa
    60361 agacatccaa gaagctatcc aattcataca acaccacggc gatagccgtc aaaagaaact
    60421 ccatttgaaa cgtcaagaac tttccttaca agacttggaa gcaaattatt ccgaagaaaa
    60481 tttcatgaat aaattagaag aagtccttga tttaaaagaa aagtgggaac agctaactga
    60541 aaaacgtgat cgtttagaag aagaggaaga atggcttctt cattggcaac agctggatat
    60601 tgctccaagt atgtatgcaa gtaagacgac catttttgag atggggactg tgagtgcagc
    60661 aaattttgaa agtttcaagg aagaactaag taagcttgat gacgtttata tggaagaaat
    60721 agattatacg ccaaaacaag tttatctttc gtatgttgct ttaaaaaaat cagttgctgc
    60781 tatagaagaa attgctggga aatacgggtt caataaagaa acgtatcctt atgaacaaaa
    60841 tcctaaagaa aaattgcaag aaaccaaaca gcaattatcc attgttcacg agcagcaaaa
    60901 aaagataacg acagcgttag gtcagtgcag cggctatatc caagaatttg aatgggtaga
    60961 agaagtcaca cttgccgttg cagaacggga aaaagtaaaa gaacggttta tccatgccac
    61021 ttatctgatc gttttgcaag gttgggtaga tgcagaagaa aaacagaatt tgatgcatgt
    61081 gttaaacgaa actgtatcag aagaagatat atacgttagt ttcgaaacac caaactcaga
    61141 agagattcag caggaagtcc ctactaaatt gaaaaaccat ccgcttatcg aaccttttga
    61201 attgctgact gaaatgtaca gtctgccaaa atatgaagaa gtagatccaa cgccttggat
    61261 gacaccattt tatcttgtct tcttcgggat gatggtcgca gatgttggtt acggattgct
    61321 gatgctcatt gggacaatac tagctcaaaa actatttgtt ttaccccgcg ggatgaaacg
    61381 tttcgtaaaa ttctttgaga ttctctcggt accatcgatc atctggggat tgatctacag
    61441 ttcatttttc ggacaggcat taccaaaaga actatttggt atccgcttgc ctttcccaat
    61501 cctatctacg acggatgacg tcaatacgat tttgatttta tccgtgattt tcggtctgat
    61561 acagatcttg gttggattat tcgtggcagc aaaggaaaat atcaggagga aagattactt
    61621 gagtgctgtc agtgatggtt ttgcctggca agggatcctg attgggatcg ttattgcatt
    61681 gattggtgtc atgttgataa aaaatacagc atttgtttat cttggcgcgg ggctatcgat
    61741 cattgctgct ttatgtatct taatcgtgcc gattatccaa tcgccgtcta aagtcaaggg
    61801 tgctgccaaa ggagcgtata atctttacgg attgactggt tatatcggtg atttagtcag
    61861 ttatacacgt ttgatggcac taggtatttc aggcggaagt attggcgcgg cattcaatat
    61921 gctagttgct ttcatgccgc cagccgctcg attcagtgta gggatccttt tgatcatcgc
    61981 tttacacgca ctgaatatgt ttttgacact gttaagtgcc tatgtccatg gtgcacgatt
    62041 gcagtatgtc gaattttttg gaaaatttta tacaggaggc ggacgtgcct ttgacccgct
    62101 gaaaacggcg gaaaaatacg tcaacatcaa tcataagaaa aaaaacaata aataattgga
    62161 ggatttttat acaatgatgg attacttaat tagtcaaaat ggtggaatgg tatttgcagt
    62221 attagcgatg gcaacagcga cgattttttc agggattggt tcagctaaag gtgttggaat
    62281 gacaggtgag gcagcagcag cgttaacaac aagtcaaccc gaaaaattcg gacaggcttt
    62341 gattctgcaa ttgcttccag gtacgcaagg gttgtatgga ttcgttatcg cctttttgat
    62401 ttttatcaac ttaggaaatg atatgtcagt tgtacaagga ttgaactttc taggcgcttc
    62461 tctgccgatt gcttttacag gtttgttttc aggtatcgcc caagggaaag ttgcagcagc
    62521 aggtatccaa attttagcga aaaaacctga acatgctaca aaaggaatca tttttgctgc
    62581 gatggttgaa acatatgcga ttttaggttt cgttatttcc ttcctattag tattgaatgc
    62641 ctaagcataa aggaggaaaa gcagatgaac gccattgaaa aaatcatctc tcagatgaat
    62701 gaagcagcag agcaagaaag agcggcgtta gagcaagaag agcgaatgaa gatcgatcaa
    62761 aactttgaac aaaaacgtac gcaaatcgaa acagaacatc aaaaacaaaa agaaaaacaa
    62821 atcgaattac ttgaaaaaaa ataccgtcag cttaggaatc gccaacaagt agaagtacga
    62881 caagaaaatt tgaatgcgaa gcaggaattt ttacgacgat tgtttgcaga tgcaatagct
    62941 gaaatggaga attgggatga atctgaacaa atccagttta tcaaaaattc attgtattca
    63001 ttacctttga caggcaaagt aacattcatt gctggagaaa aatcagcagc ttatctttct
    63061 cagacattgc tggatgaatg gaacaatgaa cttcccttta tgatggtttt aagtgatgaa
    63121 actgttgctg atcaagcggg ttttctgata aacgatcaag gagttcagta taattttctt
    63181 ttcagcagcc ttgtacaaga tatccaagga acgatgagtt ttgagattgc caatcaatta
    63241 ttcgaataag gagggatcag tgtatggaat accacgaact aaatcccttg attcgcggaa
    63301 gagagcttga acttttgtcc aatgagacat ttgaacggat gattcaaaca aattctgtcg
    63361 aagcacttgg tgaaatattg aaatccacga tttacagtcc atatatttat gacggatatg
    63421 aatatgactt cgaacagaat cttttaaagg agcagagccg tctatttcgc tggttaaagg
    63481 aatcagcacc ggagcctgaa atcgtttgga tctatacgat gcgctatact ttccataatc
    63541 tgaaggtttt gacaaaagcc gagatgacaa atcaaaatct ggaccatttg tatatcgacg
    63601 atggatttta ttcattagaa accttgaagg atgccattca tacgcaagtt tcaaatgaac
    63661 tccctgaaag cattatggac agtatacgag aagtacgtga ttattttgaa gagtctacca
    63721 ttcttcaggg aattgacgtg atttatgatc gacagttttt gacagagcag cgacggcttg
    63781 ccgataagct aggatatcaa gaattgctga cagaagtcat tgcttttatt gacttgacca
    63841 atatcacaac gatggctcga ggaatattgc agaaaagaac atatgggttc atgacgacgg
    63901 tactttccag ctcgggaagt atcgaaaaag aagtattttt gaactttgta gataaagata
    63961 tggaagatta cacgaatttt ctattagaga cagattatgc tgattttctg cgccctgtca
    64021 tccgtgaggg aaatcttgat ctcgtaaagc ttgaacagtt gaaagatgat tatctaagtt
    64081 cattgtatca agaagcacaa acccaggctt tcggaccact gccactacta gcctttttga
    64141 acgcaaaaga ggtagaaagc aaaaaccttc gtttactggt tatcggcaaa agaagccatt
    64201 tcgaaccaga aacaatcaga aagcgggtga gacaagtcta tgacgcataa gatcggtgta
    64261 gttggcgaca aagattctgt tttgccattc aaattgtttg gctttgatgt tcgttatggt
    64321 acaaccaagc aaaccgttcg tcaggcaatt gaagaaatgg caaagaatga gtatggcgtg
    64381 atttatgtca cagagcaatg tgcagcattg agcccagaaa cgatcgaacg ttacaaggaa
    64441 caaatgatac ctgctatcgt attgattccc aatcatcaag gatcattagg gatagggctg
    64501 gaagaaattc aaaaaaacgt agaaaaagca gtaggtcaaa atatcttata gtatgaaaag
    64561 gagatagaaa ttttgcaaat tggaaaaatc ataaaagtct ccggacctct tgtaatggct
    64621 gagaacatgt ctgatgccag tatccaagac atgtgtctag tgggagatct aggagttatc
    64681 ggagaaatta tcgagatgcg tggcgatgtg gcgtctattc aagtatatga agagacttct
    64741 ggaattggtc caggcgaacc ggttcgctct actggtgaag ctttatctgt tgaattagga
    64801 ccagggatca tttcacaaat gtttgacgga atccaacggc cattagatac ctttatggaa
    64861 atcacacaaa gtaatttctt agggcgcgga gttcaattgc ccgcattgga ccatgagaaa
    64921 aaatggtggt tcgaaccaac agtagaagcc ggagaaacgg tgagtgctgg agatgttatc
    64981 gggatcgtag aagaaacaaa agttataaaa cataaaatca tggtgccaaa tgggataaaa
    65041 ggaacgatca aaacaatcga aaaaggttca ttcactattg atgaaacagt atgtgtcatt
    65101 gaaaccgaaa atggtgacaa aaatttgacg atgcttcaaa aatggccagt aagacgtagt
    65161 cggccaatca aagaaaaatt aaacccggat gcacccatga tcactggaca gcgagtgatt
    65221 gatacattct tcccagtgac aaaaggagga gcagcagctg tacctggtcc ttttggtgca
    65281 gggaaaacag tcgttcagca tcaaattgca aaatgggccg atgtggacat ggttgtctat
    65341 gtgggatgtg gcgagcgtgg aaatgagatg acggatgttt taaatgaatt ccctgaattg
    65401 attgacccta atacaggaga atcgttgatg gaacgtacgg tattgattgc taatacatca
    65461 aacatgccag tagcagctcg agaagcttct atctatacgg gaatcacaat tgcggaatat
    65521 ttccgtgaca tgggttatga cgttgctatc atggcagact ccacttcacg atgggcagaa
    65581 gcacttcgtg agatgagcgg acgtttggaa gaaatgcctg gtgatgaagg ttatccagct
    65641 tatcttggtt cacggcttgc ggaatactat gaacgatctg gaagagtcat tgcattagga
    65701 tcagaaggtc gcgaaggaag tataaccgca atcagtgctg tatccccgtc tggaggggat
    65761 atttcggaac cagtcacaca aaatacattg cgggtcgtaa aagtattctg gggtcttgat
    65821 tccagtttag cccaaaaaag acattttcct tcgatcgatt ggatacaaag ctattctctt
    65881 tattcaacag aagtagggaa gtatatggat catatccttc aagctgactg ggcatccatg
    65941 gtcaccgagg gcatgcgtat tttgcaggaa gaacagcagc tgaatgaaat cgtgcgtttg
    66001 gtagggattg attccctttc agataatgac cgtctgacat tagaagtggc aaaatccatt
    66061 cgtgaagatt atttgcagca aaatgctttt gatgatgtcg atacattcac ttcaagagag
    66121 aaacaattca aaatgttgaa gatgatcttg acctttggtg aagaaggacg ccgtgcgttg
    66181 tcgttaggtg cttatttaag tgagatcatg gacggtaccg tatcgattcg tgaaaggatc
    66241 ggacgaagca aatatatccc ggaaacagaa ttagaaaaac tagatcaaat tattggtgaa
    66301 atcaagcaaa cgatgcaaac gatcgtttca gaaggaggca tgagcaatga ttaaagaata
    66361 tcgtacgatc ggtgaagtcg tcggtccatt gatggcggtt gaaaaagttg ctggtgtcaa
    66421 atatgaagaa ttaatcgaag tccgtatgca aaatggcgag attcgtcgtg gacaagtatt
    66481 ggaagtccaa gaagataaag caatggtcca aatattcgaa ggaacaagtg gtatcaattt
    66541 acgtgactct tctgtacgat ttttaggcca tcctttagaa ctaggtgtat cggaagacat
    66601 gatcggtcga gtattcgatg gtttgggacg accgaaagat aacggaccag agatcctgcc
    66661 tgaaaagcat ttggatatta acggagaagt aatcaatcca gtagctcggg attatccaga
    66721 tgagtttatc caaacaggta tctctgcaat cgatcatttg aatacattag ttcgtgggca
    66781 aaaattacca gtattttctg gctctggtct tcctcataaa gaattagctg ctcagatcgc
    66841 gcgacaagca acggtactcg attcatcaga tgatttcgcc gtagtctttg ctgctattgg
    66901 aatcactttt gaagaagctg aatttttcat ggaagatttt cgtcaaacgg gcgcaatcga
    66961 ccgttctgtc atgtttatga atttagcaaa cgaccctgca atcgaacgta tcgctactcc
    67021 tcgtatggct ttgactgctg cagaatatct tgcttatgaa aagggaatgc atgttttggt
    67081 tatcatgaca gacatgacca actatgcaga ggcattgcga gagatttctg cagcacggcg
    67141 agaagttcca ggaagaagag gatatcctgg gtatctttac acgaacttag ctactttatt
    67201 cgaacgagca ggaagaatcc gcggactaaa aggatcggtt actcaaattc cgattctaac
    67261 aatgccagaa gacgataaaa ctcatccaat ccctgacttg actggttata ttacagaagg
    67321 acagatcatc ttgactagag agctttacaa gagtgggatt cagccaccaa ttgatgtact
    67381 cccgtctctt tcgcggttaa aagataaggg aactggaccc ggaaagacaa gagaagacca
    67441 tgcagctacg atgaatcagc tttttgcagc ctatgcacaa ggaaaacagg caaaagaatt
    67501 agccgtagtt ttaggtgaat ctgctttatc tgatgtagac aagatctatg cgaagtttgc
    67561 tgagcggttt gaaaaagaat atgtcaatca aggattctac acgaaccgtt cgattactga
    67621 aacacttgat ctagggtggg aactgctatc gatgttgcca agaaccgaat taaaacgaat
    67681 caaagacgac atgctggaca aatatctgcc agaagggaag tgagttctga tggcacgatt
    67741 gaatgtgaat ccgacccgca tggaattgac tcgattgaaa aaacagttga caactgccac
    67801 aagagggcat aaattattaa aagataagca agatgaactg atgcgtcaat tcatcctgtt
    67861 gattcgaaag aacaaccagc tgcgtcaaga aatggaagag tctatgacag aagcaatgtc
    67921 tgattttgta ttggcaaatg cttcgatcac ggaacctttc atcgaagaat tatttgtttt
    67981 gccggcggaa aacgttgatt tgtctatcgt agacaaaaat atcatgagtg taaaagtacc
    68041 tgtgatgaat tttgaatatg atgaacagct gacagacaca cctttggaat acgggtattt
    68101 gaattccagt gccgaactgg atcggtcaat cgatcggttt actaccctgc ttccaaaact
    68161 tttggaactg acagaaatcg aaaaaacttg tcaactgatg gcgagtgaaa tcgaaaagac
    68221 acgtcggcga gtcaatgcat tagaatatat gacgatccct caattagaag aaacgatcta
    68281 ttatattcgt atgaagctgg aagaaaacga gcgcgcagaa gtaacaaggt tgatcaaagt
    68341 aaaaaatatg ggtaccactg actaagtggt atcttttttt aaaagaggaa aacgatgatt
    68401 aaacaaagca aacaaataaa gaaacgtttg ccggctgttc agcttctcgc attaggattt
    68461 tttctcctga ttttagttgg cggcggccta cttaccctac cattttttag taatagtggg
    68521 catgggacaa attttatcga tgcgcttttc actgcaactt ctgctgtctg tgtaacggga
    68581 ttgacaacat tgaatacagc agagcattgg aacagtgctg gtcaattttt gatcatgctt
    68641 ttgattgaga tcggcggtct tggttttatg atggttccga ttctatgctt tgcattgata
    68701 aagaagaaag tcagtctcag cacacgtatt atattaaaag aagcgctgaa tctagaagaa
    68761 gtatcaggtg tgatgaagct catgctttac atcctaaaat tggcctttgt tatccaattg
    68821 attggtacgg ttgctttatc ttttgtattt gtgcctgaat ttggctggtt gaaaggtatc
    68881 tggtacagtt tgttccatgc aatctctagt ttttgtaatg cagggtttga tttgttagga
    68941 gacagtttag ccaatcacca gcaaaatgtt tatttgatca tggtggtatc tgctttgatc
    69001 atttctggtg ggttaggctt catcgtatgg cgtgatttac tggaatatca tcagaaaaag
    69061 aagatgacga ttcattcaaa aatcgcatta accgttacag gtaccctttt attaggcgga
    69121 ttcgttgtgt tctttttcac agagcgcaat ggggcaacgc tagtggaagg gaatatgttt
    69181 caacgattgg caaacacctt ttttatgagt gtgacaccta gaacggctgg atattattca
    69241 gttgattacc tccatatgag ccatgcaggt ctgattttaa cgatgttttt gatgtacatc
    69301 ggcggaacat ctgggtcgac ggccggcgga ttgaaaacaa ctacgttagg tgtgttgctg
    69361 atccagatgc gttcgatgtt gaaaggacgt acgagagcgg aagcatttgg ccgtaccatc
    69421 cgacaaggcg cagtgttgcg ggcacttact ttattttttg ttacgttgac gttgtgtgta
    69481 gtcgccatca tgattttatc ggtgactgaa acgataccta aaacttctgg catcgaatat
    69541 attgcttttg aggtcttctc tgcgtttggt acagtaggtc ttacgatggg attgacgcca
    69601 gacttgactg caatagggaa attgatcatt atagcgctta tgtacattgg acgagttggt
    69661 gtattgactg tggtcttctc tctgatgaca aaagcacaaa gaacacaatc aaaatacaaa
    69721 tatccagaag aaagtgtcat gattggataa tttcatcttc tgacagaaac ctagtgattt
    69781 agtaaaataa gaaaataaaa taatttttct tgcttcttgg atactttaag tgcaaaatgt
    69841 tcgtttattt tttgaggcac tacccgttgt tgaacctcgg gtagtgcctc ttttttattg
    69901 aaaggaatca ataagatttg attcctttat ttatcaatag gttataattc atattttttt
    69961 attttttctt ttacgatact tagtccagaa gctaaaattt caaggttatt agaggatagg
    70021 gaaattcgaa cgaatgcatc tggttgcgca cgtgtagtaa agcgtctgga atgataaagt
    70081 cggataccgt tagcaagaaa gtcattctca attatcttag ggtcaatctc aggatcaact
    70141 ggaagaattc gatagtaagg gacacgattc gttggttttt ctactttgaa aagagaatca
    70201 aaaagctgat tggctctttt tgtaagactt aatttagttt ctaagatcct atttgcttca
    70261 tttgaatgga ggatttgagt gatgacttcc acatctaatc cagaagtttt cacatttata
    70321 ttaaaaattg cttgttctat cttgctttgg acatgtactg gaactacaag ataagcaact
    70381 cgcaatccag aacaaataaa ttttgtcatt ccagctaagt agattgtttg gttaggtaat
    70441 aaattttgga aaggaggaat gatcgtatgt tgatagtaag ttgttaaaaa tgaatggata
    70501 tcatcttcaa taacccatag accttctttt tcaatcactt cggctaattg ctctcgtctt
    70561 ttgtgagaga tttgaaaccc tataggatta ttacaagaag gcataagaaa gacaccatga
    70621 atcttctttt ttctacattc ctgaatcaat atatcaggtc gcattccttg ctcgtcaaaa
    70681 tcaattggaa cgatctctaa atgatataac tgagccaatt caatgaaatt agcgtatgta
    70741 tatcggtctg ttgctattct atcacctgga gagaaaagtg cagaaagtgt gatagccaat
    70801 ccattttgaa caccggaaac aatagataga gattctaagg acggaatgac tccttgagtt
    70861 tttaaccaat ttgctgcagt agtcaattga aaatttgtgc ctttgggatc ttggtaatta
    70921 agcaaaccaa caagatttgg gttattacta gttgattgaa taaacgggac aacgaattta
    70981 ttacattctt caaaagaact aaccagacca aaatcaatga tatgttcggt tacttcattg
    71041 gtagagatgg tgatggactc aaagacattg ggagaaataa atgtgccact tccaatattc
    71101 gtatacagca aacctttctc aattccatat ttataagcct tgccaacggt agtaaaattt
    71161 aaatctaaat aatcagctag ctctctttgg gagggtaact gagtgttttt ctgtaattta
    71221 ttctttaaaa tatcttgttc taattgttga atcaacgatc gataataagg acgcttcaat
    71281 ttttgtttgt ctggtcgcca agttaacggg taattgtcat aagaattaat aggcattttc
    71341 tccactcctt aaaattgtat accatacaat tttaccattg attgtattga ttttagaagt
    71401 taatcttttg tgaggaggga attgtctatg aaaattatta attttgagga agtagtagct
    71461 tcttttagtt ttgaagaagc gattcaagta atgaaaaaat gtttttttga ttatgaaagt
    71521 ggtaaaatca gccaaaatcc tcgattggtg gaaattttac ctgacggaaa aggcaaaaat
    71581 atgtttgcga tgatgcctgc ttatttgggg gagaacagat attttggcac aaaagtcatt
    71641 accgcttttc cggataatca tcagtatcat ttaccttctc atttaggaga gattttcttg
    71701 tttgatgctc aaaacggact acccaaagca ttgataaacg caaatgcagt cacctggatt
    71761 cgaacagctg ctgtatctgc gctggcaaca gattacttag caaatccgca tgcagaaaca
    71821 cttgctttaa ttggagctgg tcaacaagca ttgtcgcatt tgactgcgat ttctaccatt
    71881 cgtcccatca ctacagtgtt tgtttacgat attttagaag aaagacgaaa tacttttatc
    71941 gctaaagcta aaaaacagta tcctaaaata acgtttataa atgcaatatc agtagaagat
    72001 gcagtacgtc cagcggaaat catttgtacg ttgacctcta gtaaagaggc atttcttcaa
    72061 aaagagtggg taagggaaga cgcacatatc aatgctgtgg gcacatttac tcctgataca
    72121 agagagataa gtacatcgtt gatggaaact tctcaaatct atgtagatga ttactcagct
    72181 gccttaacag aaagcgggga cctgcttata gccatggcag aaggagattt agaggaatct
    72241 tgtattgttg gtacattagg cgaattagtt tctgagaaaa aaacatttta taaaggagaa
    72301 tcgaggccga cgattttcga cgcagttggt ctagctattg aagatctttg ttgtgcagaa
    72361 tatatttata acaaattaga gaaaggggct ttaaaatgaa cttagattat tacgtagttg
    72421 atgcatttgc agatgaggtg ttcaaaggaa atccagcagc ggtttatgtg ctagaagaat
    72481 ggctgccaga aggtacgatg caaaaaattg ccattgaaaa caatttatct gaaacagcat
    72541 ttacagtgaa gaaaaaccaa gaatttgagc ttagatggtt tacgccagat cgagaaatcg
    72601 atctttgtgg tcacgcgaca ttagcaacag catttgtttt atttaactat tacaaaatac
    72661 ctgatgaaac gattaaattt tctactcaaa gcggtaatct atttgtgaca agaattaaag
    72721 attattatta catggatttt cctagtatta tgccgaaaaa agtgccgata ttgtctgaat
    72781 atgaagaagc gattggggct aagattaaag aagcctactt agcacgtgat ttatttttcg
    72841 tattagaaga tgaaaaaacg gtggctaaat tgcagccaga ttttacagct atcaaaaatt
    72901 ttgaattagg aataggagtc atcgtgacag cagaaagtgt gcaaaaagac tttgtttcta
    72961 gaaccttctt tccaaaatta acaatcaatg aagatccagt atgtggatca gcacattcta
    73021 atcttattcc ttattgggca gaaagaatga ataaaacaaa attgtcagct tatcaaatgt
    73081 catcaagggg cggggatcta gaatgtgaat taaaagaaga tcgtgtgata ataggaggaa
    73141 aagcagtttt gttttctaaa ggtacctcgt ttatttgatt tctttcaaat gaattaaaaa
    73201 taaattggtg gagagtggca accttatagc tacaatggtt gttaaaaaaa gacgcaaact
    73261 ttttcaattt aatgaaaagt ttgcgtctca ttttaattat tggtgtttgt tttttattca
    73321 atcaagagta aaatgcccca tactaactat cctcggatga cttcgatttt ataaccatca
    73381 gggtcaatga caaagtagta agaaggcgca gtgccaggta atccttttaa gtcagtaatc
    73441 tcaagaccgg cagctttatg ttgctcgtgt aatttttcta gatcatcagc tgctatagcg
    73501 atatgaccat agccgtctcc tagatcatac gcaccatgat catagttgta agtcaactct
    73561 aattcatagt catccccagg aagtgtcaaa taaacaagtg taaatttgtt ttctggaaaa
    73621 tcacggcggc ggctttcttc aaaaccaaat gccttttgat aaaattcgat tgatgcttca
    73681 agatctttca ctcgaacaca tgtgtgagcc attttcatag attcatctct ccttttcctt
    73741 ttactcaatc ataacaaaaa gaagtaagga aatagttgat ttgtgcttgt tcatgagaaa
    73801 tttctcatgt aaaatggata gaaaataaaa ctgttaaggt ggaaactatt catggataaa
    73861 ccaatttttg gtgaaaaaaa gcaatttgct gattatcaaa cccgttatgc agcatatatt
    73921 gtcatagaaa gagatcagca gattgcagta atcgaggctc caaatggtgc tttttttctt
    73981 cctggcgggg aaattgaagg agaagagacg aaagaagaag cgattgtccg tgagctgata
    74041 gaagaaatgg gaattgtggc agaaatttct tcttatctgg gagaagctga tgagtatttt
    74101 tactcgaatt atcgtgcaac ttattattat aatccaggat atttctttgt tgctgattct
    74161 tggaagcgta taggagaacc aactgaaaag accaataaaa tttggtgggt gagccctcaa
    74221 gaagcgcttg tgaaattgaa acgtggtagc caccgatggg cagtcgagag atggatcgaa
    74281 aaaaataaaa actgaaagaa agcgagtaga tcagctttct ctcagttttt ttatttatta
    74341 cagtacatat tttttgattg cttcagctac accgtgctcg tcgtttgatg aagtgatgac
    74401 atttgctgct tctttgacaa gagaaacggc atttcccatt gctactccta gaccagcata
    74461 ttcaatcatg gataagtcgt tctcgttatc tccgatagcc atgatttcat cttgatcaat
    74521 accaaggtgc tgtgcgagat tcgcaactgc cgcaccttta cttgcttctt tatttagtac
    74581 ttcgaagtaa tagggcgcac ttttgaccgt tgtgtattta tcacggaaag tttgcggtaa
    74641 acgcgcaatt gctgcatcta aaatttctgg ttcgtcgatc atcatcattt tgacgatgtt
    74701 catgtctggt gtcatttctt caggtgttcg atattttagc ggcatcttga ctaaacttgc
    74761 ttcgtggatc gtataagcgc tgatatctcg attaggcgtg tagatcgctt tgtcatcgat
    74821 cgaatggaga tgactgccga cacggcgtgc catcacttcg atctctagga aatcttcatg
    74881 tgtcagtccg tgacgggcga tgattttacc tgatttcgtt tgttgtacga gtgccccgtt
    74941 ataagtaata acgtaatcat tatcttgaaa aaggttcaac tcgtttaact gttcctggac
    75001 acctggtaag ggacggccag tgcataaaac gatcgaaaca ccttttgcaa cagcagcggc
    75061 aatcgcttct tttacttctt ttgttacttc tcttttctca tttaataaag taccatcgat
    75121 gtcgatagcg actagtttga tagacataat ttcctccaat atattttaat ttatttcaat
    75181 caatgcgcca ttacggatat ggctggcaaa ttcttgataa gtttcgtcaa ataaatcata
    75241 atgatcacgc aaggacggat caaccatttc ttttggaaag aaaaatcgct catctccttg
    75301 ttcttgtccc gctaatgcag caacaagctg actaacctgt gataattcta tcaaagttcc
    75361 gtcatttcgc aaaatctcga tttgtgtgcg atgtctgcct tgttctgggc gataaaaatc
    75421 ataaggtaga tcgtagctag aattcacagc agtgtaataa acaggattat atccgacctt
    75481 ttctactaac aaagtgagtt cttggactaa ttttgaatcc cggttgccag aaaaagtagc
    75541 agatttcagt ggtttacgat tcaaaaaacg tttagctaaa tcatttaaaa taggatcagc
    75601 agattcagac cattgagtaa agtaagtgcc caagacccca tcatcaagtt tgaggtattc
    75661 ttctaaagta aagcttccgt caagaaatgg cagaagtaat tgagaatgga gttcaaaact
    75721 gctggcatct tcttgataca actcgtgtgc gcgatgaagc agatgctcca aaattacttc
    75781 cataccacgg gaaacaggat ggaagtacac ttgcacatac atttgatatc ggctaacgat
    75841 ataatcttcc actgcatgca tgccactcat ggaaaaagcg atcccgcctt cataaggacg
    75901 aatcacacgt aatattcggg taagatcaaa tgttccatat tctgttccag tgaaataagc
    75961 atcccgaagc aaatagtcca ttcgatctgc atcgatctga ctagaaatca tttggacaac
    76021 ttgtgggttg gggtaagttt tttggatcac actagctacc ttttcaggaa aaccttcttc
    76081 tactcggtta agaatctgat acacttctgt ttctggagaa gtgatgatct ctacagtgat
    76141 ggcttcatga ttcgtatgga agatatgttc aaatgtatga gagtaaggac catgaccgac
    76201 atcatgcaac agtgccgcac ataaagtcac cagccgttcc tgatcatccc agcctccgtt
    76261 gcctattttt tcttttgaaa aatttcgaga aaagatatcg cagattcgtc ttgaaatctc
    76321 ataaacgcct aatgaatgag taaaacggct atgttctgct ccgtgaaaag tgaaagaaga
    76381 agtgcctaat tgtttaatgc gtcgtagacg ttggacttct ttcgaattga tcaaatctaa
    76441 gattacttga tgctggacat ggatatagtt atggactgga tcacgaaaaa ctttttccat
    76501 aggcagcatt tgatatttat aaggggttgt cataatgtag ctcctttatg ttattacgtt
    76561 gttccattct ctggatttgg ttgttccatt gctccagctg gatttctttt tttagccagt
    76621 cggtagaatc ctgtaaatca cttgcagaat acagcttttc aaaagctttt gcaaatgctt
    76681 ctttgacttc ttttacaaaa aggggttggt caagcagttc ttcaagtgtt gccattgtag
    76741 caggatccac tgcaggatag ccatttgtac caaagtcttc ttttaaagca gattgataaa
    76801 atcgcttcat gacttgcccg cgtttttcct gattaccgtt gacacttaag tacatcataa
    76861 cagaaatccc atttttgatc cgacgctgag cgataccggc aaattttttg ccatggatgc
    76921 ttaaatcata agttccagga caataagaat gggtgatttc gaatgcttgt atcggtgcct
    76981 ttggaaaagc gtatttagtc aataggagca tttgctcgta agcgtcatct atggttattt
    77041 tttttgtgtc actttggggg aaaatcaaag aaatattcaa aattccttca tctgatacga
    77101 tccctaatcc acctgaattt ctgatcactg gagcgtaacc tgcatctttc agtgtttgga
    77161 tcccgtcact tagaaaagga gttcgaacat ccttcatacc taagatgact gtgcagtcat
    77221 attgccagaa atgtaaaaag atttgctggt gttctttaga atattccgtt aatatatctg
    77281 ttaatgcaaa aggggaaaag tcgttttttt gataaatgtg ctgatctagt cgaatcgcag
    77341 ggttcattca accggctcct ttgtataaat tcatattttc tacaatatta tactctaaaa
    77401 tcaaagagtt gttttggaaa aaaggttttc tgtaaaaatg gacaagttgg ttgacaaaac
    77461 aaaagaaact aggaacaatg tagctatgag aagtaaagga gaatgaataa tggaattatc
    77521 acaaggaaca ccgatttcaa tcaaattgcg tacaaaagta aaacagcaag gggaagtgca
    77581 agatttttat tttgatttga aagggcaaat ggtgacaatt ggcgatacgc tgtacatacg
    77641 ttataaagag attcaggagg agacgggcga tgagattcct gtaacgatca aattagttcc
    77701 agatggtcat gtacagctga tccgtgcagg agaaatgcgg atgcgtttga aatttggcta
    77761 taaagaacgg ttagaaacat cctatcgaac accatacggt atgatccaga ttgctacttt
    77821 tacgaaagaa ctgcatgtta gtttgaaaga ccgtcccaca ggagggaagg ttcgaatcga
    77881 ttatgatctg ttcatgggac cggaaaaaat cggcgaatat tatttgacct tggattttac
    77941 agcgtaaata taaaagaaaa gtttgatttt ttgtgtctga ttttgtatga taaggagtag
    78001 actgtgaaag gacgtgtcct gattggaaat tagtgtattt gaaggtgcaa acaaaagcga
    78061 attatcaatg atcgaagtag cacacgcgat cttagaacaa cgtggcgatg tcatggattt
    78121 ttctgattta gctaaccaga tccaaaacta tcttgggaaa tcagacagtg aaattcgtga
    78181 ttcactcgca caattttata ctgacttgaa tattgacggc agctttatct cattagggga
    78241 taatcgttgg ggcttgcgtt catggtatgc tatcgattcg atcgatgaag aagtaaacca
    78301 tggaatggac gaagaggatg aagatactcc acgtcgccgt aaacgcaaga aagtcaacgc
    78361 ctttatcaat gatgatgaag acgcgatcga ttacaatgac gatgacccag aagatacaga
    78421 tctgacagaa gaagatgacg atgatctgtt tgatgacgat gatgatgaag atgaagaaat
    78481 cgctgcttat aattctgact tgcaagaaat cggagcagat gacccgacag atgatgaaga
    78541 agacttgcca ggaaatatcg aagaagattt aagtatcata gacgaagacg atgatgatga
    78601 agattatgaa gaggaagaat tttctgactc tgaaaaagaa taaacattat tttcttacga
    78661 gaggttgtga cagagacgat cagcctatca gaataaggag gtgtgacacg atttttgtca
    78721 caccttcttt tttgtatata aaaatcccaa gtaaatacat aaaaaataaa aaaattgaaa
    78781 cttttagcgc accttgtacg tcatattaag tgaggcagaa aggagtggag aatgattttt
    78841 actggatata agaaaaaacg gcagatcgaa aaataccgtc acctgctgaa tggactcatc
    78901 gaaactgatt atcaaaaaat gttccgtatc acgatgaatt acgtacatga taaagaagag
    78961 gccttagatg tgatgcaaga tagttttcat aaagccctga actattttga aaaagaaaaa
    79021 gagatcgagc attttagtgc atggttttat cggatattga tccgcacggc attagatagt
    79081 tggcgcagac aaaaaaggac gcctgtggac ttatttgaaa ttcaagatat cccccaacag
    79141 ttacctttag aaacttccgt tgcagagctc catatgattt tagataaaat agacagtccg
    79201 gaaaaagaga ttctgatcct ctactttttt gaaggatttc gtttgaaaga aattgcgact
    79261 atcttggata tgaatgaaaa cacagttaaa acgaaaatgt accgctcatt gaatgcttta
    79321 cgtcaaatcc tagaatgaag gagagaagag tgccatgtat tcccaaaaag aaaatttaaa
    79381 aaaactaaag aaaacttata aagaacaagc aattcccaaa gggcttaaag aggagatccg
    79441 ccagcgtttt attatcgaag agcaatcatt tataaaagca aagcggagaa agagacgtct
    79501 ccaagggttc ggagtatctt tggctatcgt aatgattaca acaagtagcc ttctttttaa
    79561 tagtcaagtt cgaagctttg cagaagattt acccatctta ggatcagtca ttgaattgat
    79621 cttaggcgaa cgctttactg atcgatctga aaaaatcgat attcaggtac cgaagatcaa
    79681 tacacaaaat gagaaagaaa ataaaacaat tcatggatta aatcaaaaat actttcgcga
    79741 aggacaagca gattttgaaa aagctgaaaa agaatatgga aattttgaaa cggatcatta
    79801 ccaagttttg ggagattatc agaaaatcgt agacgataac cggtttctcg tgattgaaag
    79861 gcagatcacc caaacagcag cagatagcca tgttgaaaaa agatacgaca caatcgacaa
    79921 gaaaaacagt gtccagctct cacttccact tttattcaaa gatgacacct atctatctgt
    79981 tttgacaaaa gaagtcaaac ggcagatggc cgaacaagtc aaggaagatc catctaagta
    80041 ctattggact gagcaagata ttcaagaagg gacgatagaa aaaccaacac ttgttacgcc
    80101 tacacgaagt ttttatttga acaaagagca tcaattagtt ttgacttttt ctcaatacga
    80161 aattgctcct ggttatatgg gtacaccaga atttgtcatt cctaagtccg tgacaaaaac
    80221 gattttggca tcggaagatt atttggatca ttagacgaga agtagttgcg actagcagtt
    80281 tcaattttca agtaagtaca agtaaataat aagaagagaa aaagagaaag aagagatata
    80341 gtgaaaagaa attatttagt tatcagtatt attgctttat ccgtgctttt gtttttcgaa
    80401 attacttcgg tggcctcctt gatcaccgaa gtaaaaggac aaacacactc ttcggagcat
    80461 tcgtcacaat ctttcaaaca atcagaacaa tcaaccatta aaagccaatc aactgaaaca
    80521 gctaacgtca aagaagccat tcaagaatca gcggaaaagg tacaaagtca gtcatcacaa
    80581 tcaacacaag acagtacaaa aacgagccga gatccggctg tcaaaagcgt cgcaatcagc
    80641 tttgatgatg gaccaggagc gaccacaaca ccgcagcttc tgcgaatctt aaaagaaaaa
    80701 aacgtgcatg ccactttttt cgtcttagga gaaaatacgg cgcagcatcc tgaaatcgtg
    80761 aagcagacag ctgaagcagg gcacgagatt ggcaattaca catacgatca tcagaatctg
    80821 gccactcttt ctgcccagtc gatgacggag gaagtgacga aagcagacac tgagatcaaa
    80881 aaagtcactg gcaaaacccc cacttttgtt cgtccgccct atggttctgt cacgaatgta
    80941 ggagcaacga tcatccagcg accgatcatc gaatggtctg ttgattcaga agattggaag
    81001 acaagaaatc ctgatttgat tttgcagaag atccaagcaa cagtatacga tggggcaatt
    81061 attttattcc atgatatcta tcctgaaacg attcgtgccg ttcctcaagt gatcgattat
    81121 ctgaaagagc aagggtatcg catcacgaca gttggggatt tattagggca tccaacggct
    81181 gtcgagaact attatggcag aaacgatcat cggccagttc agtaagtgag acaagtatct
    81241 ttaataaata acacatttta ataagtagtt gaacacataa ctaccttact tgcaaaggct
    81301 taacaatatc tatacaggta caacaaaaaa ctgtccacca attttctttg gcggacagtt
    81361 ttcacttaaa aataaagaag caactattcg taaattatgc taaaatagca gtagaacgcg
    81421 tcctgaggga atcgaacccc cgtctcaaga accggaatct tacgtgatat ccactacact
    81481 aaggacgcaa gcactgggat aattgtaact aaacagataa aaaattacaa ggggattgga
    81541 caaaaaaatg aaatttgagc aaaatttttc acaaaaacag cagcagacac agaaactagc
    81601 catgacgcaa cagttgcaac agtccatcca agttttacaa tataacagtg aggagctgtt
    81661 ggcattcgtt gagaatcaag caatggaaaa tccattggtg gaagttgttg agccggaatg
    81721 gcagccagat tacataaaag cttcttcatc ttcttatgaa ggagaagaga caaattacct
    81781 aaatcagatt cctgatacaa aagggtcttt atttgattca ttgatcgaac aagtccattt
    81841 gaattaccga gatacttttt tgaggaaaat cgttctttat cttgtagaat acatcgattt
    81901 gaacggattt ttgacgatta gtttagaaga agccggaaaa gagattggag ctacgcctat
    81961 ccaaatgctc gatgcgttga ctctgattca gcaattagaa ccagctggag taggtgcacg
    82021 tagtttgcaa gaatgtctca tgcttcaaac agaacgagat gattatgcgc cggaattagc
    82081 ttatatcgtg ttggaagaat gttttgagga actggtggag cgaaaatgga aagaaatcgc
    82141 acaacgtttt tctgttgatc tacatgctgt tcagcagata ttcgactatt tacaaacgtt
    82201 gagtccaagc cctggccgga ttttcgaccg tagcagcgaa ttgtacatta ttcctgatgt
    82261 ccgggtatta gtggacgacg ataaaaatgt acaagttatc tcaaaccgaa aaaatcagcc
    82321 aaatatacgc tttcaagaga gttacttcaa acaaatgtca caacaagcag ataaagaaac
    82381 tgaaaattat ttaaaagaac ggaaacagga attcgaatgg ttgaagaaaa cgatcctaca
    82441 acgtggggat acaattttgc gggttgctca agtcattgtt tcccggcaaa aagctttctt
    82501 tatcgacaag gaacgtccaa tcaagcctct tacgttaaag gaagttgcaa ctgagataga
    82561 tgtgcatgaa tccacggtca gtcgtgcagt caacgggaaa tatttggaaa cagatttcgg
    82621 tgtatttgaa ttaaaaaaat ttttcactac acgaattcct acgaatagta cagaacagac
    82681 agaagacctt tcagcagata cagcgaagaa aaagctgcag gaattagtcg atcaagaaga
    82741 caaaaacaag ccgttgtcgg accagaaatt ggttgaattg ctgaaaaaag atgagatagc
    82801 gatctcaaga cgaacagtag caaaatatcg tgatttatta ggaatcccga gttcatcaaa
    82861 aagaaaacgt tatgataact aatcagtcaa aagactagaa aaagtatttc actttttcta
    82921 gtctttttag ttgaaaaacg agagcgaaac tgttagactt acagtcgtga ggttgagatt
    82981 tccctttatt gatgtgaaat tcgaatagtt aatttctttt tttatcgtat atgggtcgtt
    83041 ttaagtctac attggacgta aagtgtccaa ctaaggagat cgctatgctg gatgaaataa
    83101 aaatgattga atctgttgca ccagatatta tcgaagtgtt gcaagaacgc tataaaattt
    83161 tacgaaatat ctattggatg cagccgattg ggcgtcgaag tttatcggaa agtatgggga
    83221 tcacagaacg agtcttacga acagaaacag accttttgaa aaatctgaat ttgatcgaca
    83281 cctcaaaaag cggtatgacg ctgacaacta agggcgaaga agtttatcaa agccttgaaa
    83341 acttcatgga tcagctttta ggtacgcatc aaaccgaaca acagttggca gaatattttg
    83401 gtaaccaacg ctgtatcgtt gtttcaggaa acagcgaaga gcagaccaaa gtggctgatg
    83461 cattcggcga agcgctgagc gaagccttag accgactact tccagaaggc gagaatatca
    83521 tcgctgtgat gggaggaact actatggctg tggtagccga acaattgtct aatctggaaa
    83581 ataagaaacg gcataacctg tttgtccctg caagaggcgg gattggtgaa gcgataactg
    83641 tacaggcaaa ttctgtcagt gcaagaatgg ctgctaaagc aaacggtaac caccgcgcac
    83701 tttatgttcc ggaacaatta agtctggcaa cttacaattc tttactcaat gagccatcta
    83761 tccaagaagt cttaaacttg attagtgaag cgaattgtgt tattcatagt attgggcgtg
    83821 cattacacat ggcagcacgt cgtaagatga ctgaaaaaga attagtcatg ctgaaacaag
    83881 caaatgctgt tgcggaatct tttggctact ttttcaatga aaaaggtgaa gtcgtttata
    83941 aggtccctag aatcggcata gaattgcaag atttggaaaa agtcccgatc atcatggcaa
    84001 tcgcgggcgg aaaatcaaaa gctaaagcaa ttcgagctta tatgaaaaat gcaccaaaac
    84061 aaacgtggct catcactgat gaagctgctg caaatgagat tttaaaaggg gtaacccttt
    84121 aaaataaata tttttttgat tttcataagg aggaaatctt aaatgacagt taaagtaggt
    84181 attaatggtt ttggacgtat tggacgtcta gcattccgtc gtatccaaga tgtagatgga
    84241 atcgaagtag tagcaatcaa cgacttgaca gatgctaaaa tgttggcaca cttgttaaaa
    84301 tacgacacaa ctcaaggacg tttcaacgga acagttgaag ttcatgaagg ttcattcaac
    84361 gtaaacggaa aagaagttaa agtattagct aaccgcaacc cagaagaatt accatggggc
    84421 gaactaggcg tagatatcgt tctagaatgt actggtttct tcacttctaa atcagcagct
    84481 gaaaaacatt taacagctgg tgctaaacgc gttgttatct ctgctcctgg cggaaacgat
    84541 gttccaacaa tcgtttataa cacaaaccac gaaacattaa ctggtaaaga aacagttatc
    84601 tcaggtgctt catgtactac taactgttta gctcctatgg ctaaaacatt gaacgacaaa
    84661 tttggtgttg ttgaaggatt aatgacaact atccacgctt acacaggtga ccaaatgact
    84721 ctagacggac ctcatcctaa aggtgacttc cgccgtgcac gcgctgctgc tgcaaacatc
    84781 gttcctaact caacaggtgc tgctaaagct atcggtttag taattcccga attgaacggt
    84841 aaattagacg gagctgctca acgtgttcct gtaccaacag gttcattaac agaattagta
    84901 acagttcttg aaaaagaagt aactgttgac gaaatcaatg cagcaatgaa agaagcttca
    84961 aacgaatctt acggatacaa cacagacgaa atcgtttctt cagatatcgt tggtatgact
    85021 tacggttcat tatttgatgc tacacaaact aaagtaatga cagttggcga caaacaatta
    85081 gttaaaactg ttgcttggta cgacaacgaa atgtcataca ctgcacaatt agtacgtact
    85141 ttagaatact tcgctaactt ataagataga caaacgtaca agtgtaaatt atagacatgt
    85201 acatttaaaa gcggggaagc aatcgcgctt cttccgcttt ttttgataat aagtttattc
    85261 aggaggaaat aaaatggcta agaaaacagt gaaagatatc gatttaaaag acaaaaaagt
    85321 tcttgtccgt gttgacttca acgttccttt gaaagacggc gtgatcactg acgacactcg
    85381 tatcaaagca gcattgccaa caatcaacta cgttctagaa caaggcggaa aagcaatcct
    85441 tttctctcac cttggacgtg taaaaacaga agaagataaa gaaggaaaat ctttagctcc
    85501 agttgcaaaa cgtctaggcg aattattagg taaagaagta acattcgtac ctgaaactcg
    85561 cggagaacaa ttagaagaag caatccgcaa catgaatgac ggcgacgttg tcgtattcga
    85621 aaatacacga tttgaagata ttgacggtaa aaaagaaagc aaaaatgata cagaactagg
    85681 taaatactgg gcttcattag gcgatgtatt tgttaacgat gctttcggta cagctcaccg
    85741 tgcacatgct tctaacgtag gtatcgcttc aactggtatc ccaacagttg ctggattctt
    85801 gatggaaaaa gaaatcaagt tcatcggtga agcagtagaa gaaccaaaac gaccaatgat
    85861 tgcaatcctt ggtggcgcaa aagtttctga taaaatcggc gtaatcgaaa acttgattcc
    85921 taaagcagat aaaatcttga tcggtggcgg aatgacttac acattctacg aagcaaaagg
    85981 aatcaagatc ggtaactctc tagtagaagc tgataaagtg gaactagcaa aagaattaat
    86041 cgaaaaagca ggcgacaaac ttgtcttgcc aatcgacaat gtctgtgcac cagaattttc
    86101 aaatgatgtt gaaacacaag taatcgaagg agacattcct gatggtttga tggctttaga
    86161 catcggccca gcatcagtaa aattatttgc tgatacatta caaggtgcaa aaacagttgt
    86221 ttggaacgga cctatgggtg tatttgaaat gagtaacttt gctaaaggta caatcggtgt
    86281 ttgtgaagca atcgctaact tagaaggcgc aacaacaatc atcggtggcg gagactcagc
    86341 cgcagcagca gaacaattag gatttgctga caaattcaca cacatctcaa caggtggcgg
    86401 cgcaagcttg gaattgcttg aaggtaaaga attaccagga ctagcagcaa tcaacgataa
    86461 ataaacgacc aagtttgctc aattctgacg agtaatcgaa caaaaggtga agggagtttt
    86521 catgactccc tagccatttt tcaataaatt aaggagtgtg ttcctcaatg cgtaaaccta
    86581 ttatcgcagg taactggaaa atgaacaaaa ctgcatcaga agcaaaagaa tttgctgaag
    86641 cagtaaaaaa caaaatccct tcaaatgaca aagttgattc agtcatcgga tctcctgcat
    86701 tattcttaca agaattaatg acatcagctg aaggtacaga actaaaaatc tcagcacaaa
    86761 actgctactg ggaaaactca ggtgcgttca ctggtgaaac ttcaccagca gctcttgctg
    86821 atctaggtgt tcattatgtg atcatcggac attcagaacg tcgtgaatat ttccatgaaa
    86881 cagacgaaga catcaataaa aaagcgaaag ctatctttgc aaatggtatg acaccaatcc
    86941 tatgctgcgg cgaatcttta gaaacctatg aagcaggcaa aactgctgaa tggatcgaag
    87001 gacaaattac agctggttta gctgatcttt cagctgaaca ggtgtcaaac atggttattg
    87061 cttatgaacc aatctgggca atcggtactg gtaaatcagc tgacgcacaa atcgctgacg
    87121 aaatttgtgg agttgtacgt caaacagttg aaaaacttta tggaaaagaa gtatcagaag
    87181 cagttcgtat ccaatacggc ggttctgtaa aaccagaaaa tattgctgaa tacatggcaa
    87241 aagaaaatgt tgacggtgct ttagtcggtg gcgcaagctt gcaagcagat tcattcttgt
    87301 cattgttaga cgctgtaaaa taattgttga aaatttgcga aaaatgcaag aaatgtttgc
    87361 actaatgagc aaaattaact aaaatcgtat atgagggaaa tcccttagga aaaaacaaac
    87421 tcaaaggaga gacaaaacat gtcaattatt actgatgttt acgctcgcga gatcttagac
    87481 tcacgtggta acccaacaat cgaagtagaa gtatacacag aaagcggagc ttttggccgc
    87541 ggaatggttc cttcaggtgc ttcaactggt gaatacgaag cagttgaatt acgtgacgga
    87601 gacaaagctc gttatggcgg taaaggtgta actaaagctg tcgacaacgt aaacaacatc
    87661 attgctgaag caatcattgg ctacgatgtt cgtgaccaaa tggctatcga caaagctatg
    87721 atcgctttag acggaactcc taacaaaggt aaattaggtg cgaacgctat tttaggtgtt
    87781 tctatcgctg ttgcacgtgc agctgctgac tacctagaag tacctttata ccactaccta
    87841 ggcggattca acacaaaagt attgccaaca ccaatgatga acatcatcaa cggcggatca
    87901 catgctgata actcaatcga cttccaagaa ttcatgatca tgcctgttgg cgctccaaca
    87961 ttcaaagaag ctctacgtat gggtgctgaa gtattccacg cattagcttc aatcttgaaa
    88021 gctcgcggat tagcaacttc tgttggtgac gaaggtggat ttgctccaaa ccttggttca
    88081 aacgaagaag gttttgaagt aatcatcgaa gcaatcgaaa aagctggcta tgtacctggt
    88141 aaagacgttg ttcttgctat ggatgctgct tcttcagaat tctacgacaa agaaaaaggt
    88201 gtttacgtac tagctgactc aggcgaaggc gaaaaaacaa ctgacgaaat gatcaaattc
    88261 tacgaagaat tagtttctaa atatccaatc atctcaatcg aagatggatt agacgaaaac
    88321 gactgggacg gattcaagaa attaactgac gtattaggcg acaaagttca attagttggt
    88381 gacgacttgt tcgtaacaaa cactcaaaaa ttatctgaag gtatcgaaaa aggaattgct
    88441 aactcaatcc ttatcaaagt gaaccaaatc ggtacattaa cagaaacttt cgaagctatc
    88501 gaaatggcta aagaagctgg ctacacagca gttgtatctc accgttctgg tgaaacagaa
    88561 gattcaacaa tctctgatat cgctgttgca acaaacgctg gccaaatcaa aactggttct
    88621 ctatcacgta ctgaccgtat tgctaaatac aaccaattac tacgtatcga agaccaactt
    88681 ggtgaagttg cagaatacaa aggtttgaaa tctttctaca acttaaaaaa caaataattc
    88741 ttttctaatg aagatacatg atcgactatt gaaagatagt cgagtatgat cacttagtta
    88801 agtaatcgaa ctctctactt gctgaaatat cagcaggtag agagtttttt tgcaactagc
    88861 ttctgtatat gatgtttaca aagaatggta caagaaaaga tcatccttga aaaaggaaag
    88921 ctaacaataa ctagcatcat ataagagctt ttaaggatag aacagtagaa taggaatcta
    88981 tgggcaagtc ctctcgtata aataaaatag gagggaagta gcagcatatt tttcataaag
    89041 tggctctctt tcttcatttt tactttggtt gctcctattt gaaaattcga gcacactcaa
    89101 atacaataaa aagaaatggc aagggagagg attagatgaa aaaagaagaa aaaatcgcga
    89161 ttttacaaga gattatccga attaaatcag tgaatggaaa cgaaggtgaa gtagcagcct
    89221 atttgaataa gcttttggca aagcatgaca taacaggaga gattgtttct tacagagatg
    89281 gtagagataa tttgattgct cgttaccaaa agggccaatc aggaaaagtg ttaggcctat
    89341 ctggacatat ggatgtcgtg gcggctggtg atgaatcgtc ttggacctat gcaccctttg
    89401 cagcagaaat ccatgggaac cgtttgtacg gacgtggtgc tacagatatg aagagtggct
    89461 tggcagcaat ggtgattgcc atgatcgaac tgaaagagtc aggaaaacca tttaacggaa
    89521 cagtcaaatt attagccact gtcggagaag aagtgggtga actaggtggg gaacagctca
    89581 cgaaagcagg ctatgtagat gacttagatg ccttgatcat cggagagcca accaattaca
    89641 gtttgatgta tacgcacatg ggatcaatca actatacagt cacctcgcac ggaaaagaag
    89701 cgcatagctc tatgccagac caaggttata atgcaatcaa tcacctaaat gaattcatta
    89761 cgaaagcaaa tgctgaaatg aatcacctcg ctgaaacaat cgagaatcct gtcctaggaa
    89821 aaacaatcca taatgttaca ttgatcagtg gcgggaatca agtcaacagc atcccaagcc
    89881 atgcgcagct ccaagggaac attcgatcga ttccagaata cccaaatgat aaaatcattg
    89941 cattgctgca atcaatcgtc aatgaattga atcaagagac agactatcat ttggaattga
    90001 tgatcgatta caacaaaata cctgtcaaag ctgatccaga ttctcctttg attcattgca
    90061 ttcagcaaca attcagccag ccattacctt tagttggagc ggcagcaaca acggatgcag
    90121 ccgaatttac gaaagctaac cattccttcg actttgtcgt ctttgggcct ggtgtcgtta
    90181 ctttgcctca tcaagtagat gagtatgttg aaatcgataa ttatttggat atgatcgaaa
    90241 aatatcaagg aattatttta tcctacttag cgtaagattt ttttaataa
//
LOCUS       DAZABD010000003        89356 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000003 DAZABD010000000
VERSION     DAZABD010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 89356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 89356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..89356
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.003"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1074
                     /locus_tag="ACHALG_000178"
     CDS             <1..1074
                     /locus_tag="ACHALG_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2585623.1"
                     /translation="YVFEKINVKNLLLVCSIYCLIPLTVMGETGWRATTLNYQWPVAF
                     SLLTFYPFFQLLRGEEINRKIYWVSIPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGR
                     YNYKLSVFSIISLAELIFSLTTPGNALRAAHEINKWFPEYKNFNFLNKLDLGISSFGK
                     PFFLDMNILFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYFGNTMGQSFTYVN
                     GNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLIVGIYLSFDNKKT
                     SIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMAVKELMKSMSVQK
                     NEFMQFGLTVLGICTFIITVINR"
     gene            complement(1314..1397)
                     /locus_tag="ACHALG_000179"
     tRNA            complement(1314..1397)
                     /locus_tag="ACHALG_000179"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1361..1363),aa:Leu,seq:gag)
     gene            1470..1631
                     /locus_tag="ACHALG_000180"
     CDS             1470..1631
                     /locus_tag="ACHALG_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2585624.1"
                     /translation="MEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGIAMDWAA
                     NLWNDYHDN"
     gene            1780..2997
                     /locus_tag="ACHALG_000181"
     CDS             1780..2997
                     /locus_tag="ACHALG_000181"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFN2585625.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            3181..4017
                     /locus_tag="ACHALG_000182"
     CDS             3181..4017
                     /locus_tag="ACHALG_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN2585626.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            4093..5241
                     /gene="nagA"
                     /locus_tag="ACHALG_000183"
     CDS             4093..5241
                     /gene="nagA"
                     /locus_tag="ACHALG_000183"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFN2585627.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            5519..6520
                     /locus_tag="ACHALG_000184"
     CDS             5519..6520
                     /locus_tag="ACHALG_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFN2585628.1"
                     /translation="MTSTVFIILLGFVCRKKGIFSESVGKILSKVVLTVALPALAFRA
                     FMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYKGDKQTTLRVLTIFGSTTFF
                     GTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGLKMEAKNIKAMFVNPIIIAT
                     FAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPWLFQPMDYLAGLSSPLAWLA
                     IGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILAVLTATNILPVNFEALATIV
                     IMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVLTPVWIVVLQMIHGIGLFS"
     gene            6539..7558
                     /locus_tag="ACHALG_000185"
     CDS             6539..7558
                     /locus_tag="ACHALG_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFN2585629.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(7660..8376)
                     /locus_tag="ACHALG_000186"
     CDS             complement(7660..8376)
                     /locus_tag="ACHALG_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFN2585630.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(8345..9130)
                     /locus_tag="ACHALG_000187"
     CDS             complement(8345..9130)
                     /locus_tag="ACHALG_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFN2585631.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            complement(9121..9870)
                     /locus_tag="ACHALG_000188"
     CDS             complement(9121..9870)
                     /locus_tag="ACHALG_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFN2585632.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            10168..10692
                     /locus_tag="ACHALG_000189"
     CDS             10168..10692
                     /locus_tag="ACHALG_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFN2585633.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            10708..12207
                     /locus_tag="ACHALG_000190"
     CDS             10708..12207
                     /locus_tag="ACHALG_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFN2585634.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(12295..12897)
                     /locus_tag="ACHALG_000191"
     CDS             complement(12295..12897)
                     /locus_tag="ACHALG_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFN2585635.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            13001..13957
                     /locus_tag="ACHALG_000192"
     CDS             13001..13957
                     /locus_tag="ACHALG_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFN2585636.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            complement(14262..15218)
                     /locus_tag="ACHALG_000193"
     CDS             complement(14262..15218)
                     /locus_tag="ACHALG_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFN2585637.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            15392..15952
                     /locus_tag="ACHALG_000194"
     CDS             15392..15952
                     /locus_tag="ACHALG_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2585638.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            15945..16769
                     /locus_tag="ACHALG_000195"
     CDS             15945..16769
                     /locus_tag="ACHALG_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFN2585639.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            16779..17396
                     /locus_tag="ACHALG_000196"
     CDS             16779..17396
                     /locus_tag="ACHALG_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFN2585640.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            17414..18097
                     /locus_tag="ACHALG_000197"
     CDS             17414..18097
                     /locus_tag="ACHALG_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFN2585641.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            18780..20063
                     /locus_tag="ACHALG_000198"
     CDS             18780..20063
                     /locus_tag="ACHALG_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN2585642.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            20079..20966
                     /locus_tag="ACHALG_000199"
     CDS             20079..20966
                     /locus_tag="ACHALG_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN2585643.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            20983..21819
                     /locus_tag="ACHALG_000200"
     CDS             20983..21819
                     /locus_tag="ACHALG_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN2585644.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            21822..22445
                     /locus_tag="ACHALG_000201"
     CDS             21822..22445
                     /locus_tag="ACHALG_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2585645.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            22442..23734
                     /locus_tag="ACHALG_000202"
     CDS             22442..23734
                     /locus_tag="ACHALG_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFN2585646.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      24093..24190
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            24306..24887
                     /locus_tag="ACHALG_000203"
     CDS             24306..24887
                     /locus_tag="ACHALG_000203"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFN2585647.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            24890..26197
                     /locus_tag="ACHALG_000204"
     CDS             24890..26197
                     /locus_tag="ACHALG_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFN2585648.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMILISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            26613..27101
                     /gene="purE"
                     /locus_tag="ACHALG_000205"
     CDS             26613..27101
                     /gene="purE"
                     /locus_tag="ACHALG_000205"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFN2585649.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            27094..28218
                     /gene="purK"
                     /locus_tag="ACHALG_000206"
     CDS             27094..28218
                     /gene="purK"
                     /locus_tag="ACHALG_000206"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFN2585650.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            28231..29526
                     /gene="purB"
                     /locus_tag="ACHALG_000207"
     CDS             28231..29526
                     /gene="purB"
                     /locus_tag="ACHALG_000207"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFN2585651.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(29706..30083)
                     /locus_tag="ACHALG_000208"
     CDS             complement(29706..30083)
                     /locus_tag="ACHALG_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2585652.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            30339..31067
                     /gene="purC"
                     /locus_tag="ACHALG_000209"
     CDS             30339..31067
                     /gene="purC"
                     /locus_tag="ACHALG_000209"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFN2585653.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            31067..31321
                     /gene="purS"
                     /locus_tag="ACHALG_000210"
     CDS             31067..31321
                     /gene="purS"
                     /locus_tag="ACHALG_000210"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HFN2585654.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            31323..31994
                     /gene="purQ"
                     /locus_tag="ACHALG_000211"
     CDS             31323..31994
                     /gene="purQ"
                     /locus_tag="ACHALG_000211"
                     /EC_number="3.5.1.2"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HFN2585655.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            31995..34217
                     /gene="purL"
                     /locus_tag="ACHALG_000212"
     CDS             31995..34217
                     /gene="purL"
                     /locus_tag="ACHALG_000212"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HFN2585656.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            34202..35641
                     /gene="purF"
                     /locus_tag="ACHALG_000213"
     CDS             34202..35641
                     /gene="purF"
                     /locus_tag="ACHALG_000213"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFN2585657.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            35664..36716
                     /gene="purM"
                     /locus_tag="ACHALG_000214"
     CDS             35664..36716
                     /gene="purM"
                     /locus_tag="ACHALG_000214"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFN2585658.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            36713..37291
                     /gene="purN"
                     /locus_tag="ACHALG_000215"
     CDS             36713..37291
                     /gene="purN"
                     /locus_tag="ACHALG_000215"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFN2585659.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            37288..38823
                     /gene="purH"
                     /locus_tag="ACHALG_000216"
     CDS             37288..38823
                     /gene="purH"
                     /locus_tag="ACHALG_000216"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFN2585660.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            38839..40113
                     /gene="purD"
                     /locus_tag="ACHALG_000217"
     CDS             38839..40113
                     /gene="purD"
                     /locus_tag="ACHALG_000217"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFN2585661.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            40235..41149
                     /locus_tag="ACHALG_000218"
     CDS             40235..41149
                     /locus_tag="ACHALG_000218"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFN2585662.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(41282..42847)
                     /locus_tag="ACHALG_000219"
     CDS             complement(41282..42847)
                     /locus_tag="ACHALG_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFN2585663.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKILQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(42840..44291)
                     /locus_tag="ACHALG_000220"
     CDS             complement(42840..44291)
                     /locus_tag="ACHALG_000220"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFN2585664.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(44305..45321)
                     /locus_tag="ACHALG_000221"
     CDS             complement(44305..45321)
                     /locus_tag="ACHALG_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN2585665.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            45541..46629
                     /gene="chvE"
                     /locus_tag="ACHALG_000222"
     CDS             45541..46629
                     /gene="chvE"
                     /locus_tag="ACHALG_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     substrate-binding protein"
                     /protein_id="HFN2585666.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            46683..48209
                     /gene="mmsA"
                     /locus_tag="ACHALG_000223"
     CDS             46683..48209
                     /gene="mmsA"
                     /locus_tag="ACHALG_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFN2585667.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            48211..49416
                     /gene="mmsB"
                     /locus_tag="ACHALG_000224"
     CDS             48211..49416
                     /gene="mmsB"
                     /locus_tag="ACHALG_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     permease"
                     /protein_id="HFN2585668.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            49673..50650
                     /gene="guaC"
                     /locus_tag="ACHALG_000225"
     CDS             49673..50650
                     /gene="guaC"
                     /locus_tag="ACHALG_000225"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFN2585669.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            50914..52338
                     /locus_tag="ACHALG_000226"
     CDS             50914..52338
                     /locus_tag="ACHALG_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFN2585670.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            complement(52380..52637)
                     /locus_tag="ACHALG_000227"
     CDS             complement(52380..52637)
                     /locus_tag="ACHALG_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2585671.1"
                     /translation="MPIRILTALVNKITIRKAFYISSAFFLFSNQTYNHLFLFKNVGV
                     IQFCCKHLFYLHIVNGRTNNGFFLVSLYRILFPKKKKQLEI"
     gene            52799..53908
                     /gene="pdhA"
                     /locus_tag="ACHALG_000228"
     CDS             52799..53908
                     /gene="pdhA"
                     /locus_tag="ACHALG_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HFN2585672.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            53911..54888
                     /locus_tag="ACHALG_000229"
     CDS             53911..54888
                     /locus_tag="ACHALG_000229"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HFN2585673.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFILEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            54913..56556
                     /locus_tag="ACHALG_000230"
     CDS             54913..56556
                     /locus_tag="ACHALG_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFN2585674.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            56564..57970
                     /gene="lpdA"
                     /locus_tag="ACHALG_000231"
     CDS             56564..57970
                     /gene="lpdA"
                     /locus_tag="ACHALG_000231"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFN2585675.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            58132..58407
                     /locus_tag="ACHALG_000232"
     CDS             58132..58407
                     /locus_tag="ACHALG_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HFN2585676.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            58413..59180
                     /locus_tag="ACHALG_000233"
     CDS             58413..59180
                     /locus_tag="ACHALG_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFN2585677.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            59348..61177
                     /gene="typA"
                     /locus_tag="ACHALG_000234"
     CDS             59348..61177
                     /gene="typA"
                     /locus_tag="ACHALG_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFN2585678.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            61438..63210
                     /gene="recQ"
                     /locus_tag="ACHALG_000235"
     CDS             61438..63210
                     /gene="recQ"
                     /locus_tag="ACHALG_000235"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFN2585679.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            63324..63626
                     /locus_tag="ACHALG_000236"
     CDS             63324..63626
                     /locus_tag="ACHALG_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HFN2585680.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            63629..64792
                     /locus_tag="ACHALG_000237"
     CDS             63629..64792
                     /locus_tag="ACHALG_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFN2585681.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKNFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            64818..68246
                     /locus_tag="ACHALG_000238"
     CDS             64818..68246
                     /locus_tag="ACHALG_000238"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HFN2585682.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            68267..69391
                     /locus_tag="ACHALG_000239"
     CDS             68267..69391
                     /locus_tag="ACHALG_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP-associated domain-containing protein"
                     /protein_id="HFN2585683.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            69372..69818
                     /locus_tag="ACHALG_000240"
     CDS             69372..69818
                     /locus_tag="ACHALG_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFN2585684.1"
                     /translation="MIAVIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESA
                     KVCQLKEAFLSKKEAFENVAAYGEYAPDYREKKRVVRKAKRQLDMDEHVAAFRYSETQ
                     LQSLLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            69802..70140
                     /locus_tag="ACHALG_000241"
     CDS             69802..70140
                     /locus_tag="ACHALG_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HFN2585685.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            70259..70819
                     /gene="rsmD"
                     /locus_tag="ACHALG_000242"
     CDS             70259..70819
                     /gene="rsmD"
                     /locus_tag="ACHALG_000242"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFN2585686.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            70816..71307
                     /gene="coaD"
                     /locus_tag="ACHALG_000243"
     CDS             70816..71307
                     /gene="coaD"
                     /locus_tag="ACHALG_000243"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFN2585687.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            71297..72340
                     /locus_tag="ACHALG_000244"
     CDS             71297..72340
                     /locus_tag="ACHALG_000244"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepM family pheromone-processing serine
                     protease"
                     /protein_id="HFN2585688.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            72499..73227
                     /locus_tag="ACHALG_000245"
     CDS             72499..73227
                     /locus_tag="ACHALG_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFN2585689.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            73343..73588
                     /locus_tag="ACHALG_000246"
     CDS             73343..73588
                     /locus_tag="ACHALG_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFN2585690.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            73585..73803
                     /locus_tag="ACHALG_000247"
     CDS             73585..73803
                     /locus_tag="ACHALG_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFN2585691.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            73939..74478
                     /locus_tag="ACHALG_000248"
     CDS             73939..74478
                     /locus_tag="ACHALG_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HFN2585692.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            74471..76744
                     /locus_tag="ACHALG_000249"
     CDS             74471..76744
                     /locus_tag="ACHALG_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFN2585693.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            76782..77828
                     /gene="holA"
                     /locus_tag="ACHALG_000250"
     CDS             76782..77828
                     /gene="holA"
                     /locus_tag="ACHALG_000250"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFN2585694.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(77917..78171)
                     /gene="rpsT"
                     /locus_tag="ACHALG_000251"
     CDS             complement(77917..78171)
                     /gene="rpsT"
                     /locus_tag="ACHALG_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFN2585695.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(78571..78840)
                     /locus_tag="ACHALG_000252"
     CDS             complement(78571..78840)
                     /locus_tag="ACHALG_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2585696.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(79102..79230)
                     /locus_tag="ACHALG_000253"
     CDS             complement(79102..79230)
                     /locus_tag="ACHALG_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2585697.1"
                     /translation="MFEKLKYFIGTIGWFLLAVLLILGLAYLLFPDLIQNVFDTAF"
     gene            complement(79310..79765)
                     /locus_tag="ACHALG_000254"
     CDS             complement(79310..79765)
                     /locus_tag="ACHALG_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFN2585698.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(79903..81831)
                     /locus_tag="ACHALG_000255"
     CDS             complement(79903..81831)
                     /locus_tag="ACHALG_000255"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFN2585699.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QCKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(82028..82861)
                     /locus_tag="ACHALG_000256"
     CDS             complement(82028..82861)
                     /locus_tag="ACHALG_000256"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFN2585700.1"
                     /translation="MFFANIIKAVILGIIEGITEWVPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(83053..83757)
                     /locus_tag="ACHALG_000257"
     CDS             complement(83053..83757)
                     /locus_tag="ACHALG_000257"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFN2585701.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(83780..84406)
                     /locus_tag="ACHALG_000258"
     CDS             complement(83780..84406)
                     /locus_tag="ACHALG_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFN2585702.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            84732..87107
                     /locus_tag="ACHALG_000259"
     CDS             84732..87107
                     /locus_tag="ACHALG_000259"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFN2585703.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(87131..87622)
                     /locus_tag="ACHALG_000260"
     CDS             complement(87131..87622)
                     /locus_tag="ACHALG_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HFN2585704.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            87809..88729
                     /locus_tag="ACHALG_000261"
     CDS             87809..88729
                     /locus_tag="ACHALG_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFN2585705.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            88883..89227
                     /locus_tag="ACHALG_000262"
     CDS             88883..89227
                     /locus_tag="ACHALG_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HFN2585706.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
BASE COUNT        28992 a        15398 c        19155 g        25811 t
ORIGIN      
        1 tatgtttttg agaaaataaa tgttaaaaat ttattattgg tttgctctat atactgttta
       61 ataccattga cagtaatggg ggaaaccggt tggagagcaa ccaccttgaa ctatcagtgg
      121 ccagtagcat ttagtttact aactttttat ccgttttttc aactcttaag aggagaagaa
      181 ataaatagaa aaatatattg ggtaagtatt cctttattga tatttttaac taatcaagaa
      241 caagtcaacg cctgtttttt tgttctaaca agtatagtta gtttatattt gattgtcaat
      301 ggacgatata attataagtt atcagtattt tcaataataa gcttagcaga attaattttc
      361 tctttgacaa ctccagggaa tgcattacgt gctgcacatg aaattaataa atggtttcct
      421 gaatataaaa atttcaactt tcttaataag ttagatttag gtatttcatc atttggaaag
      481 ccgttttttt tagacatgaa tatcttattt ttattgctat ttttcttaat tttccttcta
      541 acatatagga aatgtcaaaa ctattatgtg cgtatattga ctgcattacc gtttttctta
      601 aacctgatta tttattttgg taatacaatg ggtcaaagct ttacttacgt caatggaaat
      661 aaaagggcaa tgatttggag tagtagtaac ctgaataatc tctttacaga gttaggaacg
      721 aaactgtcac ttttttatcc aggtacttgg atagctactt tagtagtctt ggcattatta
      781 ctatgtttaa ttgttggaat ttatttaagt tttgacaata agaaaacttc tattttttta
      841 gttattttga tgataatggg attttgttca aggttaatta tgggattttc tcccacagta
      901 tgggcttccg gaatgcggac gtactatata ctttatgttg ttatcgccat cttagtacta
      961 atggcagtta aagagctaat gaaaagcatg agtgtacaaa aaaatgaatt tatgcaattt
     1021 ggattaaccg tgcttggtat ttgtaccttt ataataacgg tgataaacag ataatataca
     1081 aaaaatccct cgctttagta ttaggtgagg gattttactc gtttttcgat taattgtttt
     1141 aacacttgca agtttctacg agtagcttgt ttccgacctt catatactca cctcaggctg
     1201 tttacatgat tgtacgttct ttttgtaaag gatacagtga tatgtatata atggcataaa
     1261 gaaacgttca aacaaaaaaa ctcgtgaagc aaaattaact tcacgagttg tagtgcagat
     1321 gatgggactc gaacccacac gagctaacgc tcacatgacc ctcaatcatg cttgtctgcc
     1381 aattccaaca catctgcgta ataataacac actaatgata gcgaggtttt acatagttgt
     1441 caagctcaaa aatgggagga cttattaaga tggagaaata tgacggagaa ttttcaggac
     1501 tgggaatgat cttaggtgta ttgattggac ttgcattcgg acgttttttg cttggtttta
     1561 tgctaggaat catttgtgga attgcaatgg attgggcagc gaatctatgg aatgactatc
     1621 atgacaatta agttctcaac gcttgtttgt gtttttttaa tacttacgtt tttcctaaat
     1681 aagtggcaaa attttctagg atggagtatg atattgaaat agtaaataac aaaaaatttg
     1741 gaaagttcta actttttgaa atgagggatt ggctaagtga tgagagaatt tgaaaaatcc
     1801 aacaaattag aaggtgtcag ttacgatgtt cgcggtcccg ttctagaaga agcggagcgg
     1861 atgcaagaag agggcattag tattttaaaa ttgaacacgg gaaatcccgc accttttgga
     1921 tttgaagcac caaacgaagt cattcgtgat ttgatcatga atgcccgtga ctcagaaggc
     1981 tactcagatt ccaaggggat cttttctgct agaaaagcaa ttgagcaata ttgccaactg
     2041 aaacattttc cgaatgtgac gatcaatgat atctacaccg gcaacggagt gagcgaattg
     2101 atcacgatgt gtatgcaggg gttgctggac aatggagatg aagtactggt tccaatgccg
     2161 gactatccgt tgtggacggc ttctattgca ttagctggag gcacacctgt tcattatatt
     2221 tgcgatgaag aggcagaatg gtatcctgat attgatgata tcaaatcaaa aataacgagt
     2281 cggacaaaag ctattgttat cattaatcct aataatccta ctggggcact ttatccaaaa
     2341 gaattattag aagaaatcgt tgaagtagct cgacaaaata acctgattat ttattccgac
     2401 gaaatctatg atcgcttagt gatggatggt ctagaacata ttccgattgc tacattagcg
     2461 cctgatttgt tcgttgttac gctgaacggt ctatccaaat ctcatcgtgt agcaggattt
     2521 cgagtaggct ggatggtcct aagtggagat aagtcacacg tcaaaggcta tatagaaggg
     2581 ctgaatatgt tgtcctccat gcgtttgtgt tccaatgtat tgtctcagca aattatccaa
     2641 accgcgctag gcggttatca aagtgtcgat gagctgctcc tgccaggcgg aaggatctat
     2701 gaacaaagag aatatatcta taatgcaatc aatgatattc ctggtctttc agccgtaaaa
     2761 ccaaaagctg ctttttatat attcccaaaa attgatacaa agcgattcaa tattttggat
     2821 gatgaaaaat tcgttttgga ctttttacat gaacatcata ttctacttgt acatggtggt
     2881 ggatttaatt ggaaccagcc agatcatttt cgaatcgttt atcttcctaa aatggatgat
     2941 ttgaaacaaa cagctaagaa aatgagagaa ttcctagcaa cctatcgcca aaaatgattt
     3001 taaaaaccaa atttttcagt aggagggcca cagacaaaca cactgaggct ctctttctag
     3061 tggagttttc ttaaataact aaagtatatt atttagttgc aagtcttttc ttgaagtttg
     3121 cgacttccta aagtaaaata cgaatagaat gcgaatacgc atattttggg gaggatactt
     3181 atggaaacgt taatcgagtc ctttggaacg atcattaaag agattcgaaa agaacaaaaa
     3241 atgacgcaaa aaatgctttc gcatgatatt tgttcacaaa gtgtattaag tcggatcgaa
     3301 aataatgagg aactgccgaa tgtattggtg atgatgcaga tttgtcaacg gctaggggta
     3361 acgattgatc atgtgatgaa tctctctcga aaaactactc ggacaaacga taaattattt
     3421 gaactgttgg atagttgttt ccaaaaacgt gattatcgga agttagaaca agtactaaaa
     3481 tcttctgata tttctgaaaa tctgtatcaa gcaactgact ttcaacgcta ttattattat
     3541 ctcggtgttt gcgaattcac gttgcgtcaa aatatctctc agtcccttta ttatttgaaa
     3601 gaagcattaa gctactctag ccaaaaagaa agaatccatg tttctgacac agaaatacaa
     3661 ctgattagtt gtatcggaaa aatatatggt gtggcaggaa aaacggctga agcacgctac
     3721 tatttgagtc gaagcatcca actgctgcat caatcagctg atgaacgatc aaaaagtgaa
     3781 ttttcgcaaa ttttttataa ttatggaaat ttcttattcc atcaaaatga aatcgacgaa
     3841 gcgttagaac aggtgaatca aggaatctat tgggctcaag agaagaatag ctactattac
     3901 ctcaatgatt tatttgtgct taaaagctta atatacaagc gtaaagaaga accagaaaga
     3961 gcagcattct atgaaggctt agcacaagct gtgaaacaaa tttcaaaaaa tttgtaaatg
     4021 gtgtagacca ttcttgactt cttctctaaa taagagtact ttagaggtag aaaaacaaag
     4081 ggagagaata gaatgagaac ttttatctat gctgacaaat tctttttagc gtcaggtgtc
     4141 aaacatgcag gctatcttga aattaccgat ggactttttg gtgaatacac caaagaacta
     4201 cctgagggag aagtcaacat cattgaccaa agcggcaaat ggattgctcc tggtttggta
     4261 gatacacata ttcacggcta tatgaatcat gatgtaatgg ataatgatgc agaaggcatc
     4321 aaagtgatgt ctgaaggtct gttatcttgt ggagtgactt catttttacc aacaacattg
     4381 acgtcaagca aagaacgttt aaaagatgta gctgagacaa tcggcaaaat gtatcaggaa
     4441 gcaccaggag ctaaaatcaa aggaatctat tttgaaggtc ctttcttcac agaagaacac
     4501 aaaggggcac aaaacccaag ttatttcggg gatcctgatt tggatacttt ccatgaatgg
     4561 caagaagctt caggtggttt aatcaaaaaa attgctttag caccggaacg tcaaggtgta
     4621 gaagagtttg tcaaacaagt gactgaagaa ggagtagttg tttctctagg acacagtaat
     4681 gctacactag aagaagcgca aaaagctgtt gaagcaggcg caagtgtatt tgtccatgct
     4741 ttcaatggta tgcgtggatt gaatcatcgc gaaccaggta tggtgggtgc gttattatct
     4801 ttagaacatg tcttttctga attgatttgt gatggccatc atgtacatcc aaatgctgcg
     4861 ggaatcctaa tggaaaaagc aggacatgat catgtcgcat taattacaga ctgtatgatg
     4921 gccggtggga tgcctgatgg aaattacaat ttaggcgaat ttccagtagt agtaaaagaa
     4981 ggaacagcac gtctagaatc tggcaactta gctggaagta tattgaaatt gaaagaagca
     5041 attaaaaatg ttgtagactg gggattagca acaccagaac aagcagtcat gatggctagt
     5101 ctgattccag caatcagctg taaaatcgat gatacatgtg gaaaaatcgc tcaaggacgc
     5161 gatgcagact ttatcgtatt gaaccctgat atgacattgg aagcaactta tctagatggc
     5221 gtggaacgtt tccacgcata atataattta agttaacctt aaaactaaac ttcttaagtt
     5281 tggttttaag gttttttttg tgttctagta aaaatcactt tacataagtt tcaaaagttt
     5341 ctttaatttc ctattgttca ataagtaaca atgtaaagat aaaattaaaa atgttaatac
     5401 gtattagcaa aataaaagct ttttttattg tgaataaatg aacataatta ggaggagaaa
     5461 gatgaactta tttgacattt ttcgatcgac tttgacagat atgtcgattg tcagtgcaat
     5521 cacttcaaca gtttttatta tattattagg gtttgtttgt agaaaaaagg ggattttttc
     5581 tgaatctgtt ggaaaaattc tatcgaaagt cgtcttaaca gtggctttgc cagcactggc
     5641 atttcgagct ttcatgcaag atatcaatac agaattactg atgcaaggaa tcaatgtctt
     5701 gatttgggga ttagttattt atattattct tattctagtt tcaaaacctt ttttcttaag
     5761 ctataaaggg gataagcaaa caacgttgcg tgtcctgacg atctttggtt ctacgacctt
     5821 tttcggaacg cctatcgtca gtgcgattta tggtcctgtc ggcgtgatgt attcctcaat
     5881 tttcaatatt ggctaccgaa tctttttgta ttcttatgga tacataaaaa tgagtggctt
     5941 gaagatggaa gctaaaaata tcaaagcgat gttcgtcaat ccaatcatta tcgctacttt
     6001 tgccggcctt ttcatttggc tgttccaagg gtatctgcca caagtgtcag ttacaggtgc
     6061 agaaggagag ataagccaag tagcgttttt gcgaattgac cagactgcac cgtggctatt
     6121 tcagccgatg gattatttag ctggtttaag ttcaccattg gcgtggctag caatcggcgc
     6181 aacattaggt gaagtaagct tcaaaaaagc tgcatctgaa aaaacttcat ggtattacag
     6241 tctagtcaaa gtggtatttg tacctgcatt gaatatcgtg attctagcag ttttgacggc
     6301 aacgaatatt ctaccagtca attttgaagc actggcaaca atcgttatca tgatggctac
     6361 accagctgca acagtggcag cagcctatgc tatcagtttt gatagggagg cacttttagc
     6421 ctccaatgca tccttactgt caactatttt tgctgtggta ctgacacctg tatggatcgt
     6481 tgtattacaa atgattcatg gcataggttt attttcataa gaaatggagg aagaaagtat
     6541 gagaatcgca tgttacggtg ttcgtccgaa cgagatagat ttttttgaaa aattgaatat
     6601 ttaccattat gatctcagtc tttatgaaga actgttgaca catgacaata tcgtgtcagc
     6661 aaaggaccat gatgctgtat tgttgagggg aaattgtata gcagatgaga ccaatcttgc
     6721 aaaaatgcag gaatatggga ttcgttatgt ttttacacgg actgtgggag tcaatcatat
     6781 cgatctacaa gcggcagctg attttggtat gacagtagcc cgtgtaccat cgtattcgcc
     6841 aaatgcgata gcagagttat ctttgacttt tgccatgatg cttttgagaa atactgcgta
     6901 tacgacgatc cgaacatctt tcaaagattt tcgagtggat gagcaaatgt ttagtcgtga
     6961 aatcagaaat tgcacagtag ggatcatagg aacaggcagg atagggttga cggaagctgt
     7021 tttgtttaag ggactaggag ccaaagtgat cggctacgat attcatcaga cagaagctgc
     7081 taaaaaagta ttgacgttcc actcgcttga agagttatta gccaaaagcg atattgtcag
     7141 tattcatgta cctcatatcc caggtgaaaa cgatcaaatg atcaatgaag gatttttagc
     7201 tcaaatgaaa aaaggtagca tcttgattaa tacagcccgt ggagagcttc aagataatga
     7261 agctatttta agagctttga aaagcaatca tttagctggt tttgcaaccg atgtttttgc
     7321 aaatgaaaca gaggtttttt tccgatcctt caagccatgg gagacgattc cagaccctgc
     7381 tatccagcaa ttagtagaaa tgtaccctag agttcttgta accccgcatg taggttcaaa
     7441 tacagatgag gcattatcaa atatgatttc gacaagtttc gagaactttc gtgaaataat
     7501 agaaactgga aaaacgaaaa atgaggtatc tcttcctaaa gcccgtcaat taagataaga
     7561 aaacatggat attcgtcaat aaaatgttta aaaatagtcc aagtaaacag ttgttacttc
     7621 tgtatacttg gactattttg tatgggataa ttcgatgact caacagtaat ctgtgagctt
     7681 tttccgccca ttctttgtca attcataaca ggttagcggc cggccgattt ccaaatattt
     7741 gattctttcg ttcaatatat cttgttctac taaaaaattc aaatatttct tgacggatat
     7801 acgtgacagt gagctttttt gagccagttc ttttgttgaa aaaatagtat cttcttttag
     7861 aataaatgca cttatttttt ttaaagtcag ttttgacaga cctttaggca aatcgatgaa
     7921 tgatggtatt tcgtgaatgg gagtggtgtg gttgaatact tgatcgatca atttctgatc
     7981 ggctttttca gtcgtttgga ttgtctgaga gaaagcaaga tacttttcga tcgccagctg
     8041 aaaacgttgc aaagtgaacg gtttgatcaa ataatctaag gcaccgtagc ggatcacatt
     8101 ctttatggtt tcgatatcat ctgcagcagt gatcaaaatc actgggatat catttccttg
     8161 ctgtctaagc caagataaaa aatggatacc actttcgcca ggcatgtata catcaagcag
     8221 cagcagatct gcgttttttt gtctgaaata tttttttcct tcttctacat ttctgaaact
     8281 ttcgattacg ttcatattag ggatgctttc cacaaaagta cgattcaatt ctgcaaccat
     8341 tggatcatct tcgattatca ggacatttgt catcattttg atcttccttt tttggtaatt
     8401 ctagatagac gattgtaccg ccatttgttt gtttggaaaa ttctaaaaat ccaccgtggc
     8461 gtttgacgat tgaatggatg agattcaatc caaacccacg attgattccc ttcgtcgaaa
     8521 aacctagcgt aaatattttt tctgagattt cttctgatat cccgacaccg tcatcgcaga
     8581 cggtcacgat gaatacttca gcttctgtgt catagtttaa atgaatccgg acctgtccgt
     8641 ttctttcatg tttttgtagc acggcttctt ttgcattatc aagtaagttc cctaacactt
     8701 gcaaaagtga atggatggag tcatccatct ccattttagg gataaaagaa tcgctttcta
     8761 acagcatttc aacagattgt tctttggctt cgtttatttt gcctaataaa aaaccggcaa
     8821 ttgcaggtac tttgatcaga cttgagactt ggccaatctc ttttttataa ttttgattta
     8881 gctgctggat aaaatgggtg acttcttcat acttcttcaa atcaatcaaa ccagaaatga
     8941 catgaagttt attcatgaat tcatgggttt gagctctcat ggagtttata tattgtttcg
     9001 tccccccaag ctcctccatt aatttcatta cttctgatcg atttcgtaaa gttgcgactg
     9061 ctcctaaaaa acggtcattg ctaaaaagag gtgtaacgct agaaatagtt tctatcccat
     9121 ttaaatacat ggattgagtc cgaacggtcg tttgatgctc aatcgtctct ttaaacaaaa
     9181 cgtcatatag attatcactg atttttttcc tacttcgaaa tccaatagct gctctgtttg
     9241 gtagaaaatg ttttttgcag gtgtattgat caagaggata gttttatcag aagagatggc
     9301 taagattcct tcatctactt cattttcgat aatcgttttt tcacttaact gttctgctat
     9361 ttcttgtggt tcaagtccaa gcagtacttt tttcattttt tgggcaagaa aaacggcgcc
     9421 tatgatgcct acgctaagtc caataatcaa tgaaaaaaca atcatttttt gtccatcttt
     9481 gacttgattg tcaatcgtgt tgtttgttaa gccaacacaa actgcaccaa taatctcttg
     9541 atcataataa atagggacga aatagcggaa gccgaaccct aacattcctt ctttttcaga
     9601 gaaaaattct tgtcctccaa tcactttttt cacatcttca tgacttgaga aaggaagacc
     9661 gatgtaactt gggtctgcgt gagaatatcg aatgccttca gggtccatga caacgataaa
     9721 atctaaattc gatgcaatca tgatttcatt tgttattcct tgaatcgcat gtgtcgggtc
     9781 accagtctgc agcttttttt ggatgagttt atcattggca acgattttag caatgctaga
     9841 gattttatct gacacactag atcggttcat cgaaagaata aaattcctga tcaatatggc
     9901 agaaacaatc aaagaaacga atgtggagaa acaaacgaca aggatgatcg tgttctggag
     9961 ctttaatggt tttttcttaa aaaaaggaaa cataggctac ctcgaaagaa tttattgtga
    10021 aaattttaac atgaaaacaa aaatttaaca aattgtcaac gataaacaca accttcctat
    10081 aaaaaacacc actgagtcgg taaaagaagc tatacagaat tttcaaacat tggtatgata
    10141 gacgtagaaa tggaaggagg cgtgtatatg atagggggaa tcgcacttga gacgatttat
    10201 tggtatacat tgctgatttg tgcaggattg gcagtcctat tgattatttt tggagatgta
    10261 ttcgattttg atggcccgct tgatccgatg cttattatcc cttggttaac tttcacaagc
    10321 ttatttggtt ttctaggtga gcgtttaaca gatcaatcat ctttattttt atttattgct
    10381 agtggaggca tatcgatagt gttggtattt ttgcttaatt tctatatatt gatgccgatg
    10441 aaacatgccg aatcaacgat gtcgtcttct gaaaaaactc tggaaggaca agtagctgtt
    10501 gtgatcacac cgatccctgt acgaggtatg ggagaaattc agttgaaaag tgttaccggt
    10561 tccattagtc gtccagcttg tttttacagc ccccaagaac agacagctga gcggggaacg
    10621 aaagtattga tcattgagat caaggaaaga atctgttatg tcatgcctta tgaaagcatg
    10681 ataaaagctt aaaggagcgt gtgttaaatg gaaataccag ttgtttttat cgcgatcgca
    10741 atcgcagctt tgattttact gatgttgctg atcgtctttg tagcgaagta ccagacagca
    10801 aaaccggatg aagcgttgat catcagtgga agttatttag gaagcaaaaa tgtgcacatt
    10861 gatgaaagca ataacaaaat caaaattgtg cgtggaggtg gcgcattcgt cttgccagtt
    10921 tttcaacgtt cgaatcggat cagtctatta tcgagtaaat tggacgtctc cacccctgaa
    10981 gtgtatacgg aacaaggcgt accggtgatg tgtgatggta cttcgatcat caaaatcggt
    11041 tcatctgtag aagagattgc tacagcagca gaacagtttt taggcaagac aagagaagaa
    11101 ctggaaaatg aagcaagaga agtattagaa ggacacttgc gctcgattct tggttctatg
    11161 actgtagaag aaatctatca aaatcgcgat aagttcagtc aaagcgtaca agaagtcgct
    11221 tctgtagacc ttgcaaaaat ggggttgatc atcgtttctt tcactatcaa agaggtcaga
    11281 gacaaaaacg gttatcttga ctctttaggg aaaccaagaa tcgcccaagt caaaagagat
    11341 gcagatatag cagaagcgga agctctaaaa gaaacgcgta tcaaaaaagc tcaagcagaa
    11401 aaagaatcac aagcagctga attacagcga caaacagaga tcgcagaatc attaaaagaa
    11461 aaagaactga aattagcaac ctacaaacaa gaacaagatg ttgctaaagc aaaagcagac
    11521 caagcctaca atttagaaag tgcccgagca caacaacaag taatcgaaca ggaaatgcag
    11581 attaaaatcg tagaacgtca aaaacaaatc gaactggaag aaaaagaaat cacacgtcgt
    11641 gaaaagcaat atgattcgga agtgaagaaa aaagcagatg ccgatcggta cgctaaagaa
    11701 caagaagcac aagctcaaaa agtcaaagaa gtgactgaag ccgaagcaga acgattccgt
    11761 gtggaagcac tggcagaagc ggaagccaat aagacacgtt tagctggaca agcagaagcc
    11821 gaagcagcac tagcaaaagg aaaagcagaa gccgaagcaa aacaaaagat cgccaatgcc
    11881 ttcaaggaat atggcgaagc agctgtcttg agtatggtta tcgatatgct accgcaattg
    11941 atgcgcgaag cagcacagcc gctaggaaat atcgaaaaaa tctctgttgt cgatacagga
    12001 agcagttcag gagaaaccag tggagcgaac cgagtgacaa attatgccac aaatttgctt
    12061 tcttctactc aagaaacact aaaagcgaca acaggacttg acttgaagga aatcgtagag
    12121 aatttgtcga cacgtgggac tactagccaa gtaaatatgt atgaagcgcc aaaagaggca
    12181 gaacaaaact cgatgaagga agaataaagt agtacacttc accgttcaat caaaaatgga
    12241 ggaagacttt ttaaagttct tctctccatt tttttatttt attgcaaaag aaaattagtc
    12301 ctcgctattt ttcttttgtt tttggatctt agcttctatc tcttgtaaat ttgttcttag
    12361 caatcgttct ttcttttgga gttcgaccaa atcttctgat agttcttcct tcttttctgt
    12421 ttcaggagga cgaagaaaga aattgatacc gattgcaatg aatgtcccta taaaagtatc
    12481 tagtacacgt tggatagcat acaaagcgga ttcgccttga gggatgctca acgcaatcag
    12541 caacaacgta gctattgcgg aaatgatacc ggaattattg ttaataccat ctgacacgac
    12601 gatcacaata ataactagaa taggaagcaa gatcaattct accaaaaaat cattgtgaaa
    12661 atagcttttg acaaaaaaat aaatgatagc taatgtacca cctaatgtat tgccgatgat
    12721 cctagacttt ccaaaagaaa cggttgtagt caagtcctga cgcaaagaaa aaacagcaga
    12781 taatgcagca atcatcggga ttcctcgttg agtcaaatga aaaaacaaga tacataaaaa
    12841 aacagctaag gcacttttga tcgtacgcat acctagtcga aaacggccaa attgcataga
    12901 gattctcctt tttggtcttt ttactatcat acctaaactt ttgtctgata gctagttttt
    12961 cattattcaa gtataattaa aataaaaaag ggggaagtaa atgcgtatag gaatgcgaac
    13021 agtcaaaaca gtgatcagtg tcttcttttc gatggtcaca gcgggtagct tatcgctgtt
    13081 gtattggcca gctgcaggga tcattgcatt attaagtgta ggaaatacca agcgctctac
    13141 attgcgtacg ggcatgaatc gaatcgcagc ctttatatta gctacggcaa cagcactgtt
    13201 cagttttttt gctgttgggt atactatttt tggcttcagt ctttttttat tcctgttcat
    13261 cccgcttgcc tctcgcttca aattaaccga agggatagta gtaaattcgg ttttggtcac
    13321 tcattattta gtggaggaaa acatgtcttg gcagctgatc ggcaatgaag cgggattaat
    13381 ggcgattgga ttagtatttg ctttgcttgc aaatgtttac atgccagatc gtacgaagca
    13441 gctgaaggaa aaccaaatac agatcgaaaa agaatttcga aatattttga gacaaatggc
    13501 agaatttctt ttatcagaaa ataaagaaga tgtgcagatc aaatgcgaac atttactgac
    13561 attcattcgc gaaagccaag aagatgccag agaacatcaa gaaaactact ggctcagaca
    13621 gccgctatac tacgaaactt acttctctat gagaagagca caggccaatg tgattaaaga
    13681 catgctggaa aatctagaga gaatccagca accagcttat tatggaaaac atatctatgg
    13741 tttattgatt tatactgcag aaacattttc agagtcaaat gatggcagac aaattttaac
    13801 acgtattgaa gaagtttatg tattgtatcg gcaaatgcct ctgccgacta gtcgccctga
    13861 attcgaggat cgagcagaat tgttccagtt tttgcagtct tttaaaagtt ttattgaaat
    13921 caaggcagaa ttctcccaac aaaaaatcca aaaatagagg ttgtgaattc agctaatatt
    13981 caggtcaaaa agctgatttc acaacctctt ctttaaacgg tgttctcctg tcaactcctc
    14041 ggtcacaagt cacaatattc aacaaacatg agaagctgtt ttttgaaatt gctccttgtg
    14101 actcggagct gaacggtagg ctcacaacct ctttcttaaa cggtgttcaa aagctttcct
    14161 ctgcggtaat aagtccagcc aaacgaaaaa taggaggagc tatttttgtt tggctgttct
    14221 tattacttga tgcagaacag ctttttcaca accttttaaa attacatatt tttccataga
    14281 acttgcattt gatccaagat ttcgatgaca tcttttgtca agaagagaga gcgattgggc
    14341 aagtcacctt caatcatgtg taccatattc tttatttcgt aattcattgc ttcagatgta
    14401 ttcccgcttt cgatgaattc ctttgtacca tctgtaaata agatctccgc acgatcagca
    14461 cgcgggtaat cagcaatcgt cacataaccg ttctcaaagg caacgatacc gacttttggc
    14521 atttttgcct gaaaagtcaa gctgacagca gcaagttcat tttctttgtt acgcaagata
    14581 gtgacggatt gttcgtccac gcctgtttca aaaggaacca ttgtagaggc aactacttca
    14641 ggttgagagc tcaaaaagaa acgagcgaaa gatacagcat acgtaccgat atccaacaga
    14701 gctcctccag ctaattctgg attgaagaac cggtttttag ggtctggttc tttgtaactg
    14761 ccgaaaggtg cttggatcat ttttaaagcc cccagttttc ctgtatccat tattgagcgt
    14821 aactgctgat aaagtggcat gttgaagatt gtcatcgctt ctgctaagat caaattcttt
    14881 tcttctgcta agcgcatagc gtcagccagt tcttttttat tcatcgtgat ggctttttca
    14941 catagaacgt gtttgcctgc ttccaatgct gccaagatat ggtcgatatg ctgtctattt
    15001 ggtacagcaa tgtacacgat gtcgatttca gcatcgtcta acatttcttg atagcttccg
    15061 taagcttttg gaatattgta acggtgagcg aagttttccg ctttttctga agttctggaa
    15121 gcaactgctg ttagttcgct cgtctcttga ttgaagcttt cggcaaactc atgtgcgatg
    15181 gtaccaagac caacgattcc ccattgatat tttttcatct gaaaacgctc cttcatagtt
    15241 ggattgagtt tcttgtctgt ctattattat aacaaaaatc acgaagaata aagaggagag
    15301 gacttgaaac attaaaaaaa atgaaagcag cgatttaaga ggtgcttttt gatcaaaact
    15361 taagtagaat agttaaaagg aggggataaa cgtgcatcaa gtatatattg atcttattat
    15421 gagtgtatta ttagaacagt ttgaaactga aaaaaatttt ttagatgaat acttgctgat
    15481 cagtccagaa caatggcaaa aatggaaaga tggcgagaaa aatctgccat ctgaagcgat
    15541 gcagaaagta aaaaatcttt tcactgatta tgaatggatg ctaacgcaaa agattttgcg
    15601 tcaaacgatt atttttcccg aaaaacgaaa cgttgccgtt gcagagtaca agcaaatcaa
    15661 aacacgcatc gcgcaaaaat ggcttagcag caaagcagca aaagtcgagc tgatcccttt
    15721 gaaaaataat cagcaggctg gtagatacct tgacttgaaa gtaagtattc aatatgatga
    15781 atggggctat gatgatattt tgaattttcg tctgccagca gatgttcaaa aacagatcga
    15841 aggagaaaaa atcgaacttc tcgattgggt gaatgaaaat ctggaagaaa cttacgttca
    15901 tgaggcgaat ggggaacgtg aagaacaacg aaaggatttt tcaagtgaat aaatttttag
    15961 gatttggagc agttgtgatt atgatcgcag caatgggcta tgttattttt agcgatgatg
    16021 attcgcatgt tcaagccggc aatcaagcag tcgaacgaac aagcaccaaa acaactgcta
    16081 gtaaagaaaa gaacagtcaa acaaccagca gcaaggtaaa ggacaaagaa acaaaagatc
    16141 gtacgtccaa acaattgcca gatgttaaaa cagacgattg gaagctagtt ttggttggtc
    16201 cagatcacaa attagaaaaa gaaattgatg agtctactca attggcatca ttaagtaacg
    16261 gatacttgat cgacaaacgc attgctccag aatatgaagc atttgccaag gctgctgaag
    16321 cagctggatt tccattagtg atggtctcag cttaccggtc tgtatcatcc caacaacaag
    16381 ttttcgaaca aaatgtccaa gatgtgatga gtcgtcaagg tgtatcagaa gaggaggcga
    16441 caaagattac taaagaaacg attacggagc caggatacag cgaacaccac acaggtctgg
    16501 cagtcgatgt cgtcgatcaa aactggtaca acagttatcc ttctactgta ttggatgcaa
    16561 gttacggaga tcagcctgga gctaaatgga tcgcagataa tgctgcgcag tatggattta
    16621 tcgttcgtta tccaaaagga cacgaagata taactaagat cacatatgaa ccttggcact
    16681 tacgttatgt cggtaaagaa aacgctgagt atatcaccaa acaccaattg acgctagaag
    16741 aatatctgaa tgaattaaat gaaaagtagg aattgatcat ggcgaaaaaa aagtggaaaa
    16801 agcaaaagcg caagaatatc cgttggccag cagtggttgc cggtgtagca gtgatactct
    16861 tagcctttgt tttttccttg aagaatctgt cttctccttt tactgataat cagcaagaaa
    16921 atgaagcgca atcccatcag caatttattg aacggcttgt tccgcatgcc caagaactac
    16981 aagatggtta tggagtgctt cctagtatca ttttaggaca agctatatta gaatcaaatt
    17041 ggggaaaaag taccttggct agcaaataca ataatctttt cgggatcaag gcttatgggg
    17101 atcagaagaa agtaagcctg gaaacaaagg aatttgttaa cgaagaatgg ataactatcc
    17161 aaggagactt caaagtgtat gattcatggg aacagtccat ggatgaccat acacaactgt
    17221 tcgtccaagg agtagattgg aatcctgcgc tgtatgaaaa agtgattacg gccaccaact
    17281 atcaggaagc cgcacaagca ttgcaagatg caggttatgc gacagacccc ggttatgcgc
    17341 aaaaaatcat ccaggtcatc gagacgtatc aattgaatca gtatgaccgg agatagcaaa
    17401 ggagaagtgg ctgatggcag aacaatttac gccagaaatc atgacggaat tgagaaaaga
    17461 tatcgtaaga gtaccggaaa tcattttaga agcaagcggg atcaagatat ttggaaaatt
    17521 gatccgttcg atcatcttta ctacggatat tgcgatcata cgtaatacaa atgcgaatgc
    17581 agtaattgct gtgtatcctt ttacacctca tccagcgatt actaaaagca ttattgaggc
    17641 agctgacatt ccagttttct ccggtgtagg tggtggtttg acacaaggtg ctcgttcttc
    17701 ctacatgagc atgttcgcag aagcgcaagg ttcgttaggg gtcgttttga acggtcctac
    17761 gcctttagag acagtaaaag aagtttgtcg agtagttgat attccagtta tcagtacggt
    17821 aacgtcgaaa tatacagaaa tagatgaaaa gttagcagct ggtatttcga ttatcaatat
    17881 cagtgcaggt aaagaaactg cagaagcagt acgccatttt cgtaaaaaat atcctgaact
    17941 tcctatcatt gcgacaggtg gtccaacgga agaaagtatt gctgagacca ttcaagcagg
    18001 agcaaatgcc atcacatata cgccaccaag tagcggagag ctttttagtc gtaaaatgga
    18061 aaaatatcgg gaaactgaga aaaagaatca tgaataacag gtgagaatca aaagacgagg
    18121 aagagcagat agtggctctt cctcgtcttt tgtactccag tactatttgt tcagaattca
    18181 aaaaaaagag aaattttttt gtttttttac ctgtaatgta cataagaaga gttaaaacct
    18241 tttgttatta ttagcattct aacaaaaaaa tgtcttgatt tattgaagcg ctttctatag
    18301 aatgaaaaac gaataaaaac taaaaaaggc acccgacaat ttagtagcag ctaaattgtc
    18361 ggggcttgaa aagtcagcgg gaactgacca atcaagttgc catagtcaat gcttgtgcat
    18421 cgacgtgtat attgtaccta atttcataca gaattactag ttatttttct gttttttagg
    18481 gaagagcgca cacgaagaag catgcttatt tagctatgtc aaggatattg ggcagttgtc
    18541 tgatatcctt ttttctttgc gtaattatcc tcccatcatt gaagcaagaa gtgtttttag
    18601 cgatgaaaga agaatcagca ctatctaatt tttcttgaaa gtattgagtt gttcatccta
    18661 ggcagaacga taaggcaagt cttggttttt attccccttg aaaaagatcg tttaataaat
    18721 atgttctgta taaaaataca gtatattgaa aaataacaat ggaagtagag gtgcagagaa
    18781 tgaagaaaag aatgagaatc atcttagctt taagtgttat gttattcttc ttgacggctt
    18841 gtgggcaaga agatgacaaa aaaatcacgc tgaacatggc ttggtggggt tcgcaagtcc
    18901 gacacgatgc aacagtaaaa gtaatcgagc tttatgaaga aaaaaatccc catgtgacga
    18961 tcgattatga attttatgat tatgaaggat attacacaaa attaggtaca ctagcagcat
    19021 cagatgatat ctgggacatt ttccaactga acaatcaatt tcctcaatac atcaatcaga
    19081 ttgaaccatt agatgaatat gtggagcaag ggataattga tatttctaat gcagatgaat
    19141 cctatttagc aacgacgact tacgacgggc aattagttgg actgtctaac ggggtgaatt
    19201 cgtttgcgat tgcttacaat aaaaatatat ttgaacaatt aaatatccct gaaccagctt
    19261 caaattggac atgggatgaa tttgaacaaa tcagctatca aatcactgaa gaattaggaa
    19321 tctatgctat ttcaagattc gaagatttta tagctggctg tattattcaa ataccacaag
    19381 ttgagaaagg attgaatttt tataatcggg aagatctatc taacagcttg ggatttgaaa
    19441 atccagacta tctaacagat tttttccagc tgcgtaaaaa tctggtagac aaaggggccc
    19501 atccagaacc aggtggtgga gctgcaagta cgacggatct gtcttttcag gcggttaggg
    19561 atagagaagc ggcaatggtg tttctatcaa gtaaccagtt gacagaaata ttgtcagggg
    19621 ctcctgaaga tatggagatt cagttgatta atccacctcg cagaaaagca gatggagaat
    19681 ctggtatccc tttacgttct tcgcaaatgc tgagtatggc aaaaagctca gcccataaag
    19741 aagaagctgc taagtttatt gatttctttc aaaacagtga agaagccaat gaaattttaa
    19801 aaggagaacg tggtgtacca atcatgtcga atattcgaga acagctcgct cgtgcagatg
    19861 atgcaacttt gcaagcgaca tttgattatt tagaaatgat ttccgatatg gacaacggcg
    19921 aagtgaatgt ctttgatagt gctttgaatc cggaaattca agaccaatac aatcttttag
    19981 ttgaaaaagt catttatgaa gaagtaacag cagaagaagc agcagagagt ctttatgagt
    20041 ttgcgtcaaa cattctagga tagcagcagg aggaaggcat gaaaaaagaa aaactaaaac
    20101 ggttcttgga tcaagacaat gtggtcggtt acgtatttgc ttctccattc atcctaggat
    20161 ttctattgat aacagtgata ccgatgatct tgtcacttta ttattcgttc accgattatc
    20221 gtttgggaaa tccagtctca tggatcggct tggataatta tataaagatg ctgagtgatg
    20281 cccgtttttt gaactccatc cgagtcactt ttctctatgt atttgcatct gtacctgtga
    20341 aactgatctt tggtctattg attgcttatc ttttaacgag gaatatcaaa ggaacgactt
    20401 tctttcgttc tttgtattat gtgcctagtt tgatcggtgg cagtatttct atcgctctag
    20461 tttggcggga gttgttttct aaaaacgggt tagtgaatat tttcctggaa cgattaggcg
    20521 cacctgcgct ttcctggttt agcgatcaaa aactggtcat gatccctctt gttttgatgt
    20581 ctgcttggca attcggttcg gcaatgatca tctttgctgc aggtatcaaa gagatcccag
    20641 gctcgtatta tgaagcggca gagatcgatg gagcgaacaa atggcagacg ttttacaaga
    20701 tcacgttacc tcttctttcg ccgatcattt tgtataactt ggttatgcag acaatcgcgg
    20761 cgtttatgac ttttacgcag gcatttatca ttacccaagg tggtccgaat gatgccacga
    20821 acttttatgc tctttatgtg tataaccaag ctttttcttt taattctatg gggtatgcta
    20881 gtgctatttc gtggatcatg ctgttgatta tgggagctgt cacctttctg attttcaaat
    20941 catcaaaaaa atgggtcgtt gtgtgaatga gaggaggaag aaatgaataa tacagtaacg
    21001 aaatggtttc aatacggttt tattttgcta ttaggtctag tcatgattta tccggtctta
    21061 tggatgatcg caggttcttt taaaacggat caagagatta tgagtggttc gctgaatttg
    21121 attccagaga cttttcaatg ggaaaattac gcaaaaggat gggcggggtt tgctggaatt
    21181 tcatttttta ctttctttag gaattcgttt gtcatctctg cagtttcgac gatagggaca
    21241 gttctgagct cgacttgtat tgcttacgca ttatcgagaa tcgatttcaa aggaaaaaga
    21301 ttctggttcg tcgtcatgtt agtaacgatg atgattcctg cacaagtgat tttgattccg
    21361 cagtttatta tttacaatcg gctgaatttg gttgggacgt atgtgccgct gattttacca
    21421 catttttttg gacaagcatt tttcatttac cagatcatgc aattcatggt cagcatcccc
    21481 aaagagttag atgaatcagc gattattgac ggatgcagca agtatagtgt ctttaccaaa
    21541 attatttttc ctttgctgaa accttcgatc atcacgacga tcatcatcca gttttattgg
    21601 aaatgggacg acttcatggg accattgatt tatttaaaca agccgagatc ttacacggta
    21661 tcgattgcca tcaaattatt tgcagacgct ggatctagta cgacttacgc gagtatgttt
    21721 gcgatgtcta cgttgtcgct gctaccggtt tttctgattt tcttattctt caataaatat
    21781 cttgtccagg ggatcagtac aagcggtctg aagggctgaa tatgaaaaag ccaatagaag
    21841 agatgtttct ttacaagctc aactactatg tctgtcggat ttttttaacc agtcttatca
    21901 gtaatgctct tttcgcttta gttctaggat gtttgttcac tttaggatta gtgctgtctg
    21961 ctcgtgttgt ctatctgatt tattttttga tgatgtatcc ttcttattat ctatctgtcc
    22021 attacttggc agaaaaaaag gtatgttgga ggaaaggaaa aatcaaacgg atgtgtttca
    22081 tcattacgtt gtttcagctg tttgtcatta tttttgcagt tgattggtat ttattagtgg
    22141 gaaagattgg agcttggtat ctgattcctt ttctattgtg tctgttgctt tttgtatccc
    22201 atagtttttt tagtacgcta attgatatct gcaaagaaga taaagcagat agtcttgttt
    22261 atttgatcaa aaggaatatc tggctgttgt caaaaaacgt caaggggttt ctgtggtatt
    22321 tatgcctttt gtatggactg ttctggtgta tacagcatga ttttttactc actgcattac
    22381 tgtatccagg gctgccattt gtcacagtat tttgcttaaa agaaaaggag aatgacttta
    22441 aatgaagaaa aaatatgtac aagtcggact tggtgggaga gcgcgttttt tttatgaggc
    22501 aattgcctct acgtatcatc aaacttgtga actagtcggt ttttgtgatg tatctcaaac
    22561 aaggatggat tatgcaaatc ggatacttgt gaacaagtat ggacagaaaa aagtaaagac
    22621 atgtatgcca tcacaatttg agcaactact ggatgacgta cggccggctg aagtcattat
    22681 tacgtcagta gatcgaacgc atcatgatta tatcattcgt gcaatgaaaa aaggatatga
    22741 tgttatttgc gaaaaacctc tgactatcga tgaggaaaaa gctcaagaaa taataacggt
    22801 ccaaaaacag acgggaagaa gcctgcgagt cacttttaat tatcgttatg caccgtatca
    22861 cacgaagata aaggaactga tagaaagtga tgtgattggt gatgtgtttt ctatccactt
    22921 cgaatggctg ttaaatacgc aacacggggc agattatttc agaagatggc atcgaaataa
    22981 gcacaatagc ggcgggcttc ttgttcataa atctacccat cattttgatc tagtgaattt
    23041 ttggttgaac agccgtcccc aaaaggtatt tgcattcggt ggattaaact tttatgggat
    23101 gcataatgcg gagaaacgcg gagtagaaat attttacagt cgatgctatg gaagcgaact
    23161 agctaaagct gatccatttg ctatagacct gtcagccaat gaggaattgc gagaaatgta
    23221 tttagaagca gaaaaagaca gtggatatat tcgtgaccgc agtgtatttt ccgatgatat
    23281 ctctattgaa gatacactag gcgtaacggt caaatatgaa actggtgcga ttttgacgta
    23341 ttcgctaaac gcgtatatgc cgtgggaagg attcaatgtt tctttcaatg gcagcaaagg
    23401 acggatcgaa atgaaagtga tcgaaaagtc ttacatcaat gcaggtggag ataaaaagga
    23461 tgaaggagca gccgttcttc agtccatcca tgtctatccg atgttcggtg agccatatga
    23521 ggtaatcgtc gaacaagaag cgggcggaca tggcggagga gatccgcgtt tattagatga
    23581 tctatttggt gaagaaaaag aagaagatcc gtggaatcgg gcagcaactc acgtagatgg
    23641 tattttgtcg attttgacag gaatcgcagc aaatcattct attgcttcag gaaaagcagt
    23701 agtgattgac gagcttgtca gcttaaaaga ataagaatag atttacggaa attacttccc
    23761 gtagtttata aaatcttgcc ttattgccga taggctgtct tttgaacact atttattcgg
    23821 aaaaaggggc tatgacatgc tttttgtcat gccttctttt tatgtgggct gcttttttag
    23881 ataggagaaa ataaaaataa ctatttaaac ccgaacaaaa aggaataatg cattaaaaat
    23941 cattagattt tacaaaaaaa taagtaaacc acgttgtcag tttcagaaag tagtgttaga
    24001 ctgttttcaa tatttgaaac aacatttttt attcaaatat ggcaaactgc cttcacgtgc
    24061 aaaacagttg caagaaaatg aatttacact attattagtt ttacatacct acttatatat
    24121 cgtcataata tggatgacag tttctagcca gtaccgtaaa tgctggacta taagtaaaag
    24181 attggctatt tcacccttct tttcttatga taaagtggag ggtgtttttc tttaacagaa
    24241 acatagtagg tttttgcaca aaagcggtta tttcggtatt aataaatgat aaaggagcaa
    24301 ataaagtgaa agaattagtt gaacgtattc aaaaagacgg acgtatatta ggcgagggcg
    24361 tattaaaagt cgacagcttc gtcactcacc aagtagaccc tgtattgatg gaacaaatgg
    24421 gaaaacggtt tgcagaagta tttaaagaac aaggaatcac gaaagttgtg accattgaag
    24481 cttctgggat cgcaccagct ttatttgctg caagagaatt agatgttcca atgatcttcg
    24541 ctagaaaagc aaaaagtttg acgatggatg aagaattatt gacagcttct gtttattctt
    24601 ttacgaaaca agtgacaagt acgatttcta tttctcgtaa atttttatct tcagaagaca
    24661 aagtattgat tatcgacgat ttcttggcaa acggacaagc ggcaaaagga ttgatcgaat
    24721 tatgccagca agctggagcg caagtagaag gaatcgggat cgtcatcgaa aagtctttcc
    24781 aagatggacg tcagttactt gaagaaatgg gattgagagt cgtttctctt gcgcgtattg
    24841 cttccttgac gaatggacaa gttgagtttc ttgaggagga cgcataatcg tggaaaaaga
    24901 aacacaaaat ggaaaagctg cggttttagg gctgcagcat ctgctagcga tgtatgcagg
    24961 agcagtagcg gtgcctcttt tgatcggtac aggactaggc tttaatcaag aacagatgac
    25021 ttatttgatt tcaattgata tctttatgtg tggattagcg acattattgc aattgacagt
    25081 taaccgattt ttcggaattg gcttgcctgt cgttttagga tgtgcgatcc aagcagtagc
    25141 tccactgatc ttgatcggaa ccaatgaagg agtaggagct atctacggat ctattatcgc
    25201 ttctgggatc tttgttgtgt tagtttctgg tgtcttttct aaaattaaaa aactattccc
    25261 tcctatagta acaggaacgg ttatcaccgt tattggtttg actttgattc ctgtagcaat
    25321 cgagaaaatg ggtggaggta atgcaacagc agctgatttt ggtgacaaaa caaatctgct
    25381 acttgcattt gtgacgattc tcctaattgt aggcgtgcaa atgctggcaa aagggttcat
    25441 tcgttcaatc gcggtattga ttggtttagt cggaggaagc atcttagctg ctttcatggg
    25501 aatgattgat ctgagcgcga ttggagatgc gccgttgttc catgttccac aaccctttta
    25561 ttttggtaaa ccaactttcg acgtatggtc gattttacta atgatcctca tttccatggt
    25621 cagcatggta gaatcaacag gtgtatattt tgcacttggt gacattactg ggaaaaatgt
    25681 tggagaagaa gaattgaaaa agggatatcg tgcagaagga cttgccgtta tcttaggcgg
    25741 tattttcaat acattccctt atactggctt ttctcaaaat gtcggattgg ttcaactttc
    25801 gggaatcaaa acaagaaaac ccatctattt ctctgcattc ttcttgattg ttttaggatt
    25861 attccctaaa atcggtgcgt tagcacaaat catccctgag ccggtacttg gtggaggaat
    25921 gttagtcatg ttcgggatgg tggcagtcca aggaatccgt atgctgttgg aagttgattt
    25981 cagcaatgat aaaaatctat tgattgttgc tgtttctgtt ggatttgggc tgggtttcaa
    26041 tgttacgccg acattattca gtcaaatgcc gcaaacattg caaatgttca ctggaaatgg
    26101 catcgtaatg agtagcatta cggctatcat attgaatttg atctttaatg gattgaaaaa
    26161 agatgaagaa atattggaag aagccttcaa gaagtaatag agcaaaagaa aggttgtgaa
    26221 atcagcgttt tgacctgatt gttagagaaa caaacggaaa aatagttcct cgtattttgc
    26281 cgtttgtttg agctaatacc gagggtcagc tgattgaaca ccgttgaagg aagaggtcgt
    26341 gagcctgccg ttcatctccg agtcacaagg aacaatttca aaaaaaagct tctcatgttt
    26401 gttgaatatt gttccttgtg accgaggagt tggcaggaga acaccgttta tccataaaaa
    26461 ggaatgtgac ctaacttttg tcacattcct tttttttatc tttaataaac acgaacaata
    26521 aaatgttttt ctttgttaac attcgaaatt tctttgacat caaaaaaagt gattgttaaa
    26581 atatgaatgt agaaaaactg ggaggaaaaa caatgacagc aaaaatctct gtgattatgg
    26641 ggagtacttc agattgggaa acaatgaaag aagcatgtca aatattggat gaattcggtg
    26701 tgtcttatga aaaaaaggtc gtttctgctc atcgaacccc agatttgatg tttgaatatg
    26761 cacaaaacgc tcgaaaaaaa ggaatcaaaa tcattattgc aggagcaggg ggagccgcgc
    26821 atctgccggg tatggtcgca tcaaaaacaa ctttgccggt tatcggtgta ccggtccaat
    26881 cacgaacatt aaatggtttg gattcgcttc tttcgattgt ccaaatgcct ggaggagtac
    26941 ctgtagctac tacagcaatc ggaaaagcgg gagcaattaa tgctggacta cttgcgatac
    27001 agatgctttc catgtatgat ttagaattgg aggctaaatt ggctgaaaga cgttcaatgt
    27061 tggctaaaac tgttatagaa agtagtgatc aacttggtta aacctttgtt gcctggttca
    27121 acgatcggga tcgtcggtgg cggtcaatta ggtcgtatga tggcgattag tgcaaaagaa
    27181 atgggcttta aagtaggtgt gctggatcct gtgagcgatt gtccagccgc acaagttgca
    27241 gattggcaca ttgaaagtgt ctatgatgac acctttgcgc tgaaagagct tgccagaaga
    27301 acagatgtca ttacctatga attcgaaaac gtcagtgtag aggcgttaaa tgcgatttta
    27361 cccatgtcat ttattcctca agggacagac ttgcttgcga ttacgcaaga ccgcctgtta
    27421 gaaaaatctt ttttggaaac gaataacatc gtgattgctc cttatgcaac gatcgtcagt
    27481 ccgacggata tccaagatgc gattgacggg atcggctatc cttgtgtttt gaaaaccact
    27541 cgaggcggct atgatggcaa ggggcagtat gttttgaaaa gtcgggcgga tctagctcct
    27601 gccatggacc ttttaagaga aggaacctgt gttttagaag catggatccc gtttgaaaaa
    27661 gagatttcga tcatggtggc aggaaatggt caaggagatt tcacgacttt tccggtcgtg
    27721 gaaaacatcc accacaacaa tattcttcat gaaacgattg ctccagccgc gattgatcag
    27781 gatgtgatcg aggaggctga gcgtattgcc cgtgtgattg cagaagcagt gagcctttca
    27841 ggtgttttag gggtagaaat gtttttaaca aaaacaggcg ggctatatgt gaatgaatta
    27901 gctcctcgtc ctcacaattc tggacattat tctattgaag cttgttctat gagtcagttc
    27961 gatgcccata ttcgaggtat ttgtggttgg ccattaggag aaaaagtacg tttgataagc
    28021 aatgcagtaa tggtcaatgt tttgggagaa caactgctgg aaagctatcg cttgattgag
    28081 aataagccag actggcaatt ccatttctat ggaaaaaaac aagcaaaaaa aggtcgcaag
    28141 atgggacata tcactatctt gacagaagac atccagcaaa cgctggaaga aatcaaacaa
    28201 acgaatattt gggattagag gagaaaaaac atgttagaac gttatacacg ccctgaaatg
    28261 gggaatatct ggacggaaga gaacaaatac cgtgcgtggc tcgaagtcga gattttagca
    28321 gatgaagctt gggcagaact tggtgaaatc ccgcaagaag atgtgaaaaa aattcgtgag
    28381 catgcatcat ttgatatcga tcggatcttg gaaatcgaaa aattaacacg ccatgatgtc
    28441 gttgcgttta cacgagcggt ttctgaaaca ttgggagaag aaagaaaatg ggtccattat
    28501 ggtctaacaa gtacggatgt cgtagatact gcttatggct atctcatcaa gcaagcaaat
    28561 gatattttac gggaagactt gaaacgtttt acggaaatca ttggcgaaaa agcaaaagaa
    28621 cacaaatata cagtgatgat gggtcggaca cacggtgtcc atgctgaacc aactactttt
    28681 ggattgaaac ttgccacatg gtattcagaa atgaaacgga acatcgaacg atttgaacat
    28741 gcggcaaaag gagtagaagc aggaaaaatc tcaggagcag tgggaacgtt cgcaaatatc
    28801 ccgccattta ttgaagagta tgtatgtaaa caattgggga tccgtccaca agaaatctct
    28861 actcaagttc tccctcggga tctacatgct gaatatttct cagcgatggc gcttattgct
    28921 acaagtattg aacgttttgc aacagagata cgcggattgc aaaaatccga aacacgtgaa
    28981 gtagaagaat tttttgcgaa aggtcaaaaa ggttcttctg ctatgccgca caaaaggaat
    29041 ccgatcggtt cagaaaatat ggctggtcta gccagagtgg tgcgcggaca catggtcact
    29101 gcctatgaaa atgtcacatt atggcatgaa agagatattt ctcattcttc agcagaacga
    29161 atcatcttgc ctgataccac gattttattg aattacatgt taaatcgttt tggcaatatc
    29221 gttaaaaatc taacggtctt tcctgaaaac atgaagcgga atatgtctgc aacatttggt
    29281 ttgatttata gccagcgcgt tatgctgaaa ttgatcgata agggaatgac aagagaagaa
    29341 gcttatgatc ttgttcagcc aaaaactgcg tacgcttggg acaaccaagt gcagttccgc
    29401 ccgctgttag aagaagatcc aaccatcaca tcggtattat ctaaagaaga aatagacgat
    29461 gcctttgatt atcatcatca tttgaaaaac gttgacgtga tattcaaacg agtaggtttg
    29521 gaataaagaa gagtgagttg caaaattcaa ttaaaataga atagcaaaaa taaccaaaaa
    29581 agaagcagat ccgttagcgg ttctgcttct tttagtttat tatattttct tatcaataat
    29641 aggaacctga ttttaaaagc ggctacctct ttaccgtgcc tctttacata caaaaaatag
    29701 aggaattaag cgtaaatggc ttggctatta taactaaaat gctggttatg gagatgttgc
    29761 agcaagtttt cgagaaaagc gtattccatc tcttgtccat tgaataagat ctggatacga
    29821 taatgatttt tgaaacgctt ctgttcatga atctgactga ttgaaagttc gcctttgtgt
    29881 tctgtctgat aaagaaaagt ccttttacgg cattcagcac agcgcttggt tgctcggcgg
    29941 atttttcgga atgtataaaa actagtatct ttattgcata aataaacgtc tttgatcgac
    30001 agcaaatcat tatatagaca ggaacgttct tcagctgtta attctgataa aaaaagctga
    30061 ccttgtttta ttgcatcttc catcggcaag tgcctcctcg aacccattat ccaaattttt
    30121 agttaattct aatataccat cattaagtat aaatgtaaac gcttgttgat ggtataagat
    30181 tttcgtttct gataaaacac gaacattaaa agaatctaat gccttaaatg tacatatttt
    30241 tgttgattta taaaaagatg attgctaaaa tttaaagtga aaaaacgaac aaaaaacgat
    30301 gtatttgctt ttaatgtaaa taaaaaggag agacgtacat ggagaagaaa gaacttttgt
    30361 atgaaggaaa agccaaaaaa atatatctta cttctgatga gaacttgatt tggatagaat
    30421 atttagatca agcaactgcg ttgaatggcg cgaggaaaga ccagataagt ggaaaaggag
    30481 aattgaataa tcaaatcaca agtgcaattt tccgttatct gactcaaaga ggaataaaaa
    30541 atcattttat tgaacaattg tctaaaaccg aacaattagc aaaaaaagta caaattatcc
    30601 ctctggaagt ggttatccga aatattgcag caggaagttt ctcaaaacgt ctaggtgttc
    30661 ccgaaggtaa agaattcatc aaaccaatca ttgaattcta ctataaggat gaccagttgg
    30721 atgatccatt tatcaatgaa gagcatatct atttcttaca acttgcaaag ccggaagaaa
    30781 tcagtgagct gaaagcccaa gcgttggcta ttaatgaaat tttgaccgaa ttatttgcta
    30841 aggtcggttt ggttttagta gatttcaagc tagaatttgg ccggtcgaat ggacaaattt
    30901 tgcttgcgga tgaagtaaca ccggatactt gccgattatg ggatgaacgt acgaatgaac
    30961 atatggacaa agacgtttac cgcagagatc taggtgattt gatcccagtc tatcaagaag
    31021 tatataaccg tctatcggaa ctaactgaag aaaaggagcg agtatgatgt atttcgtaaa
    31081 agtatatgta acctataaag attcagtgtt agatcctcaa ggggaggcag taaaaaaagc
    31141 agtccatcgt atgggatatg aaacaatcga agatatccga ataggaaaat actttgagat
    31201 acaacttgct gatgcagaag aaccaatcga tacagtgatt gaaacgattt gtgacaagct
    31261 gcttgcaaat gtaaatatgg aaacataccg atatgaaatc aaaaaaatgg aggaagtata
    31321 acatgaaatt tgcggtcatc gtttttccag gttcaaattg tgacgcagat atgttatggg
    31381 cagttcgcga gatcatgggc gctgatgccg aatttgtacg gcatgatgag tcctctttag
    31441 aaggatttga tggggtttta ctgccgggag gattctccta tggtgactat ttgagatgcg
    31501 gtgcgattgc tcggttttct acaatcatgg aagaagtgat tcgttttgct gaagaaggga
    31561 aaccagtatt cggtacatgc aatggattcc agatattgac agaagcagga cttttgccag
    31621 gtgtactgag acgaaatgag tctttgcatt ttatttgcaa aactgtacca ttgaaagtca
    31681 accccaagac ggcctttact tcagagtatg aagccgacga aataatctat cttccagttg
    31741 ctcatgggga aggaaattat ttctgtgacg aagaaacact tcaagaacta aaaaaaaata
    31801 atcagattgc ctttacttat aatgacaaca tcaacggcag cgtggaaaac attgcaggga
    31861 ttacgaacaa agcagggaat gtgttaggaa tgatgcctca tccagaacgt gcagtagaag
    31921 acttgctagg atcagaagat gggaaacggt tctttgcttc cattttaaaa aacttcggaa
    31981 aggtgaccgt atagatgaaa acattgaaag aaccgacagc tgaagagatc aagaaaaaaa
    32041 gaatctatgc cgaatgggga ttgactgatg aagaatatcg aatgatcgaa gaagatatct
    32101 taggaagaat gcctaattat acggaaactg gactgttttc tgttatgtgg agcgaacatt
    32161 gttcctataa aaattctaaa ccggttctac gcaaatttcc tactagtgga cctcatgtgt
    32221 tgcaaggccc tggagaaggc gcaggcatcg tcgacatcgg tgatggacta gcagttgtct
    32281 tcaaagcaga aagtcacaat catccttcag ccgtggagcc ttatgaagga gctgcgacag
    32341 gagttggcgg aatcatcaga gatattttca gcatgggggc tcgtccaatt gcgttgctag
    32401 attcattgag gttcggcgaa ttagataatc caagaacgaa atatttacta gaagaagtag
    32461 ttgcaggtat cggcggatac ggcaattgta tcggcatacc gactgtaggc ggcgaaattg
    32521 catttgatcc ttgttatgaa ggaaacccat tagtcaatgc aatgtgcgta ggtttgattg
    32581 aacacaaaca tatccagaaa gggcaagccg ctggcgtcgg taattcgatc atgtatgtag
    32641 gtgcaaaaac aggtcgcgac ggtatccacg gagcaacttt tgcctcagaa gaatttaatc
    32701 aagaagaaga acagcaacgt tctgctgtac aagtaggcga tccttttatg gaaaagctgc
    32761 tgcttgaagc gtgtttggaa ttaatcttgc atcattcgga ttctctaata ggtatacagg
    32821 atatgggagc agcaggactt gtttcatcta gtgcggaaat ggcttcaaaa gctggctcag
    32881 gacttatatt gacattagat gaagtcccac aaagagaaac tgggatgaca ccttatgaaa
    32941 tgatgttatc ggaatcacaa gaacggatgt tgatttgtgt taaatcagga gaggaagaaa
    33001 aaatccaaga actatttcag aagtatgatc tagatgcagt cactatcggt aaagtaacag
    33061 acgacgggca atatcggcta tatcatcaag gagaagaagt agcaaatctg ccagtcgatg
    33121 cattagctga agatgcacca gtttatcata aagaaatgaa agaaccagct cgaatcgtag
    33181 aatttcagca gttagctcct tatcagccag ttatcgaaga acctggacaa gtattgcttg
    33241 atctgctgca gcaaccgacg attgcttcaa aacgttcaat ctacgaaact tatgattcac
    33301 aagtacaaac caatacagtt gtccgtccag gaagcgatgc tgctgtgatg agggttagag
    33361 ggacgaataa agcgttagct atgaccacag actgcaacgc gcgctatctg tatttggatc
    33421 caaaaatcgg aggacaaata gcagtggcag aagctgctag aaatattatt gctagcggtg
    33481 gtaagccctt ggcgatcacc gactgcctga actatggttc tcctgataaa ccagaagttt
    33541 tttgggaatt atcgacttca gctgacggaa ttgctgctgc gtgtgaaacg ttaggaactc
    33601 ctgttatttc aggaaatgtc tctttatata atgaaacgga tggtcaagca atctatccaa
    33661 caccaatgat tggtatggtc ggattgattg aggatcataa acatatcact actcaggaat
    33721 tcaaaaaatc aggggatcta atctatatat taggtaagac gtttgcagat tttgatggca
    33781 gtgaattaca aaagatgcag cttggaagaa tcgaaggtgt gatccgaaac tttgatctat
    33841 ccattgaaaa gagaaaccaa gagttagtat tgacagccat ccaaaacgga ttgattgaaa
    33901 gtgctcatga ctgttcagaa ggtggcttgg caattgcact ggcagaatca gctttcaaac
    33961 atcagctagg gatatctgta cagtttgaat tgtcctctgc tcaattattt gcagaaactc
    34021 aatcaagatt tgtgttgaca gtcgcaccgg aaaataagac acgattcgaa gaaatgatgg
    34081 gagatgctgc tgttttagca ggtaaggtga cggatgaagc gataatcgaa atttcagcaa
    34141 ctgacggtca gatcaaaata gaaactgcag tagcaagaaa gtgctgggag gatgcaatcg
    34201 tatgtcttat gaagtgaaga gtttaaatga agaatgcggg atcttcggtg tttggggtca
    34261 tccagacgcc gctagtgtgg catacttcgg gctccatagt cttcagcatc gaggacaaga
    34321 aggtgcaggt atcgtgtcca atgatcgagg aaagcttaag gggcatcgag atttggggct
    34381 gctttctgag gtattcaaag atcaaagaaa attgcagcag ttaacaggag aagctgcaat
    34441 cggacatgta cgttatgcaa cagcaggcaa tggaagtgtg gacaatattc agccttttct
    34501 attcaaattt catgatcagt cgatcggctt agcgcataat ggtaacttga tcaatgctcg
    34561 ttcccttaga aaagagttag aggaaaatgg ggcgatcttc cattctaact ccgatacgga
    34621 gatattgatg catttgatcc gacgaagtga agaaacagat tttttaaata aattaaaaag
    34681 tgctttgaat gaagtcaaag gtggctttgc ctatttatta atgacagaaa cggcaatgat
    34741 tgccgctttg gacccaaatg gttttcgtcc attagcaatc ggacaaatga aaaacggagc
    34801 atacgtgatt gcctcggaaa cttgtgcatt agaagttatt ggggcacgtt ttataagaga
    34861 tgtccagcct ggtgagatag tgatcatcaa tgacgaagga atccagattg attgctttac
    34921 gaaggacacg cagctttcta tttgttcgat ggaatatatc tattttgcca gaccagattc
    34981 aaacatcgca gggatcaatg tgcatactgc acggaaaaat atgggacgga atttagcgaa
    35041 agaatcgcca gtaaaagcag atatggtcgt tggcgtacct aattcttctt tgtcagccgc
    35101 aagcggttat gcggaagcta gtggaatccc ttatgaaatc ggattagtaa aaaatcaata
    35161 cattgcccga acatttatcc agccaacgcc tgagctaaga gagcaaggtg ttcgaatgaa
    35221 attatctgct gtacgaggag tagtggaagg taaaaaggtg attatggtcg atgattcaat
    35281 tgttcggggt acgacaagca gacggatcgt ccatcttttg aaagaagcag gggcaaaaga
    35341 agtccatgtg aggattgctt ctcctccttt gaaatatcca tgtttttacg ggattgatat
    35401 tcaaacgcgt aaagaactca ttgcggcaaa tcacacgatt gatgaaatca aagaatgtat
    35461 cggtgcagat tctttaggtt ttttaagtga agagggtctg atcgaatcga tcggtctgga
    35521 aaaagatgct ccgtattcag gattgtgtat ggcttatttc aacggagatt atccgacgcc
    35581 gttatatgac tatgaagaaa aatatcagga atcactaaaa gaaaaagtat cgttctttta
    35641 aaagcagacc aagaagggag tatatgaatt caatggcaaa tgcatacgca aaagctggcg
    35701 tagatgtcga agctggatat gaagtagtag aacggatcaa gaagcatgtc aaaaaaacgg
    35761 aacgtttagg tatgatgggc gctataggag gattcggcgg ttgttttgat ttatcggtct
    35821 ttcaattaaa agagccagtc cttgtctcgg ggaccgatgg cgtggggacg aaattagcag
    35881 tggcaatcga agcagacaaa cacgatacaa tcgggatcga ctgcgtagcg atgtgtgtca
    35941 atgatatctt agcacaaggc gcagagccgt tatatttctt agactactta gcaacgggga
    36001 aaaatgatcc gttacgtttg gaaaatgtag taaaaggtgt agcagatgga tgtgttcaag
    36061 gaaatatggc gctgattggc ggagaaacag ctgaaatgcc tggtatgtac tcaggtaatg
    36121 attatgatct agccggattt gctgtaggga tagctgaaaa atctcaatta ttgacaggag
    36181 aaacgatcaa cccaggagat gtgttgcttg gaatagcatc tagcgggatt cattcaaatg
    36241 ggtactctct tgttcgcaaa attttctttg agcagcagca gttttcttta tcatcaaaac
    36301 tgccagaatt gaaaaaaaca cttggcgaag aattgcttga gccaacgaaa atctatgttt
    36361 cttccttgct tccgctgatc aaagaaaaga aaataaaagg agctgcacat atcactggtg
    36421 gagggtttgt agaaaatatc ccgcgaatgt taccagaatc tatggctgca caaatcaaac
    36481 ttggctcatg gccgattctt ccaatattcg gctgtttaca aaaatatggt gaaattcctg
    36541 agatggagat gtatgaaatc ttcaatatgg gcatcggtat ggtcctggca gtcgaagaat
    36601 cgaaagtaaa agaaatagta cacgaaattg agcaagctgg cgaaaaagtt tatgaaatcg
    36661 ggaaagtgat cgccaaagaa aaacatcgtg tggtgtttat aggagaagaa cgatgagaat
    36721 cgctgttttt gcttctggaa acggaagcaa ctttcaagcg ctagccgatt atctatcgaa
    36781 aaaaggattg gaatcttcga ttgactggtt gttttgtgat cagcctgaag catatgtttt
    36841 gaaacgtgca actgcgttga gtgtaccggc agattgtttt tcacctaaag aatttgattc
    36901 aaaaaaagag tatgaagaag caattttgca taaattgaaa gaaaaaaaga tcgatttgat
    36961 cgtattagct ggatatatgc gaatcattgg accggtattg ctgaaaaatt atgataaaag
    37021 aatcatcaat atccatccgt ctcttttacc tgccttccct ggtttgcatg gcatacgtga
    37081 tgcctttgaa gctggagtaa aagaaacggg tgtcacaatt cattatatcg accaaggtgt
    37141 agatactgga ccaatcatcc ggcaggaaaa agtgagaatt gaacaggagg acacgttcga
    37201 ttctttagaa gaaaaaatcc atagagtaga gcatcgaatc tatccagaag tgattagtga
    37261 aattattgaa aatggaggct atctgaaatg acaagagcat tgatcagtgt atcagataaa
    37321 actgggattg tagcattcgc ccaaggtttg attgaacaag gagtagaaat catttcaaca
    37381 ggaggaacaa agcatgcgtt ggataaagct gggatcaaaa cgctttcgat cgaagaagtg
    37441 acagggtttc ctgaaatgat ggatggccgt gtcaaaacac ttcatcctaa aatccatggt
    37501 ggtcttttag gaagacgcga tctccctagc catatggagg caatggaaac ggaaaatatc
    37561 caaccaatcg actttgtttg tgtcaattta tatcctttca aagaaacgat tatgaaacca
    37621 gatgtcgaaa tagcagaagc tattgaaaac attgatatcg gtggtcctag tatgctccgt
    37681 agtgcagcaa aaaaccatga atttgtcacc tctgttgttg atccatctga ctatgaagaa
    37741 gtactttcag aattacgtca gtatgggcat acttcacttt ctttcagaca aaaactagcg
    37801 gcaaaagtct ttcgtcatac tgcggcatat gatgcactga tcgctcaata cttaacggat
    37861 tgggtggaag aaaaagaacc ggaaaaattg acacttacgt atgaacgaaa acaaacgctt
    37921 cgttacggtg aaaatagcca tcaagccgct gccttttatc agtccgctct tctggaaaaa
    37981 tattcgatcg cagcagcaaa acaactccat gggaaagaac tttcttataa taatatccga
    38041 gatgcagatg ctgcgctacg gatcatgaag gaatatgacg agccaacggt agtcgccttg
    38101 aaacacatga atccttgtgg tattgggaca gcatctacga ttttagaagc gtgggaagca
    38161 gcgtatgaag cagatcctgt ttcaatcttt ggtgggatca tcgttttgaa tcgagaagta
    38221 gatctgccaa cagcagaaca gatgcataag ctgtttctag aaatcatcat tgccccttct
    38281 tatacacaag aagctttgga attactgcaa aaaaagaaaa acatccgatt actgacagta
    38341 gactttgcgc aggaagaaaa tcaaaatcga gaagaaacgg tctcagtctt aggtggactt
    38401 cttgtacaag atcaagatct ggcaatagaa aatgaagaag agtggaaagt cgtgacaaaa
    38461 cgtaagccga ctccagcaga agcaaaagca ttagcgtttg cttggagagc tgtcaaacat
    38521 gtgaaatcta atgcgatcgt actggcaaac gaacgtcaga cagtaggaat cggagccggg
    38581 cagatgaatc gagtcggttc agtaaaaatt gcgattgaac aagcgcaaga gaaaatggaa
    38641 ggtgcagttt tagctagcga tgcttatttt ccaatggatg attcggttga atatgcagcc
    38701 aaacatggta taaaagcaat cattcagcca ggaggaagca tcaaagacca agattctatt
    38761 gatatggcga ataaatacga tctagcaatg gtctttactg atgttcggca ttttagacat
    38821 tagagaggag tcggataaat gaacattttg gtgatcggtg caggtggtcg tgaacatgca
    38881 ataagtaaaa aacttttagc cagtccgaag gtggatacgg tgtattgtgc gccaggaaat
    38941 ccggggatga caaaagatgg tatcaattgt gtcgctatca gtgagcgaga tcattcagcg
    39001 ttaatccatt ttgttaagaa agaggctatt gattggagta tcgttggtcc tgaagttcct
    39061 ttgttaaatg ggatcgtcga tgattttatg gcggaaggat tgaaaatctt cggtccgaca
    39121 aaagcagcag ctctgatcga aggttcgaaa agttttgcta aacaattaat ggatacttat
    39181 caaatcccga cagctaaatc cagaagtttt gaatgttacg aaaaagcagc agcgtatatc
    39241 aaagaaaatg gtgcgccaat cgtagtgaaa gcggacggct tagcagctgg gaaaggtgta
    39301 gtcgtggcta ccacagtgaa ggaagcgctg gatgcggcag aacaaatgtt gaaccagcat
    39361 cgatttgggg atagtagcaa aaaaatcgtc gtagaagagt ttttggcagg agaagaattc
    39421 tcccttttgg cgtttgtccg taataccaag gtgtatccga tggtcatttc gcaagatcat
    39481 aagcgggcat ttgataacga taaagggcca aatacaggcg gaatgggtgc ttattcacca
    39541 gtaccgcaaa tttcaaaaaa gatgattgaa aaagctgtgg aagaagtttt aaaaccagcg
    39601 gctttgggaa tgaaagaact aggacgctct ttcacgggtg ttttatatgc gggtttgatt
    39661 gctacgaagg aaggaccaaa agtgatcgaa ttcaatgcgc gtttcggtga tcctgaaacg
    39721 caagtcgttc tttctcggtt gaagagtgac tttgcgcaag taatcgatga tttattagag
    39781 aatcgtacag tagagttaaa gtggcagcaa gaaggataca atgtaggcgt tgtggttgca
    39841 gctgccggtt accccgaaga atatgagaca ggaatggttc taccagattt tctggaagaa
    39901 gagctttctg tttactatgc tggagtttcc tcaaataacg gcaaactagt tggagcagga
    39961 ggacgtcttt atttagtaga agcatacggt gagacgatca aagaagcaca gaaaaaaatt
    40021 tacaccgcgt taatggataa aacgacagat ggaacatttt atcgtacaga tattggagaa
    40081 aaagctgcca atagcgaact gatcgtgaag taaatcagct gcttccattt ataaaaaata
    40141 caacaaacat aatcggaagc gccgatttta gtgaagcctt ttcataaaga gtgtggtaca
    40201 cttttgttgt gtatataaat aaggaggagt tcagatgaaa aaaattcaat taggtacttc
    40261 tgatcagaaa gcagctgcag tgatcttagg ctgtatgcgt atcaacaatg cggaagatcc
    40321 agtaaaagtg attgagacag cttatgaaaa cggcattgat ttttttgacc atgcagatat
    40381 ttatggaaac ggtgaatgtg aagaaatctt tgcagatgct ctagcaaaaa catcaatcaa
    40441 acgagaagat ctttttattc aaacaaaatg tggaatcgtt ccaggtgtga tgtttgattt
    40501 ttctaaaaaa catatcattg aatctgtaga aaaaagtttg acccgtctga aaatggatta
    40561 tgtggatgct ttacttcttc atcgtccaga tacattggta gaaccagagg aagtagctga
    40621 agcgtttgat gaattggaac ggtcaggaaa agttcgttat tttggcgtca gcaatcaaaa
    40681 accaatgcaa atcgaattgc tgaaaaaata tgttcgtcaa ccattagtag ctaatcagct
    40741 gcaatttggc ttgaaacata ctggaatgat cgatcaaggg attcatgtca atatgacaga
    40801 tgaagcaagt atcgatcatg acggtagtgt acttgattat tcccgattgc atgatatgac
    40861 gatccaagca tggtcaccat accagtatgg attttttgaa ggtgtattta tcgggaacga
    40921 aaaattccct gaattaaacc aaacattaga agaactagca gctaaatatc aaacgacacc
    40981 aactggcttg gcttctgctt ggattttacg ccatccagcc aatatgcaag tgatcgccgg
    41041 tacgatgaac gtagggcgaa tcaaggaaat cgctcaagct tcagaaatta atatgtctcg
    41101 ggaagattgg tacgaattgt accgtgctgc tggaaacatt ttaccttaaa gattgtcata
    41161 agcaggtcaa aagggctata aattaaaaag acagaataac ttgaaagctg ttttcagctt
    41221 cgagatattc tgtctttttt tattgcaaat ggaaagagta tcattgtacg gatttcgttt
    41281 attagttatt tttgttttta aggaaaagct gtgtccgata ttcttttgga gaaatccctt
    41341 gttttttctt aaataaataa ctgaaatagt tagggtcatt ataaccaatt tcaaaagcaa
    41401 tatctgaaat acgatagtct gattgctgca atattttttt tgctaattcg attctttgtt
    41461 ctgtcagata atcgatgaat gtttggttaa acgattgaga aaaaatcgta ctgaaatgag
    41521 agggacttaa tgcaacttgt tgagcgaccc aagttaagga catatctgga tcggtaaaat
    41581 tttctttgat aaaatccaat gcatggttca aaattggctg atatttgttc cttgaaggct
    41641 gtgagggcag agcagttaag aaagaaatga acatataaac tgtcgaacgg taaagcgagt
    41701 cgtcggcgct gattgctaag cgttgactgg tggatgtgca atgaaccatt tcttgcggga
    41761 ttttcttttc tttcaggcag acatgctgga gtagcttagt caagacaaat aaacgatata
    41821 atcgtgttcg agttggtgtg ccttgtggtt tcgtcaagtg ttcgatgtac ttagatactt
    41881 cgtttttcga taatgaagac aaatcaagtg agaggtcaaa gtaaaagaca gaatcaactg
    41941 gaagtttcgc ttcttgaatc aaatcctcat agctgataat ttgttcttta agggaactgg
    42001 cggtttcaag tatcatctgc gtgatctcaa aagatgcagg taattcgctg attctttctg
    42061 cgacaggacc aatggcgaca gaaaaattat ttttttgtcc cttttctaat tcatggacca
    42121 acgtttgggc tacctgataa ctggtctcga tcacattttc ttcaagagaa tcaactatta
    42181 aaaatttgat gaatcgagag gaaacagcag agaagatcac ttgttgattc tccctaaata
    42241 aaaacttcaa ataatcactg agatgagcgt aatcttcaaa gtctgtactg acttggtttg
    42301 ctgctaataa tacggtcatt tttttccctg ctaaagagcg attgaaagat tcagcaagtt
    42361 tcaatgtttc actgatcgta agtgagcctt tgaacaaacc gttcaagaaa tgattttttt
    42421 tcaattcgaa aacaagatca gaagaagtgt gcgtttcttt ttgctgcaaa tctaaagcag
    42481 ttactgcttt ttctattgct tgtttcaatt cgctactttt aatcggttta agcagataat
    42541 catctgcctg tacctggatt gctcctttca tataatcgaa atcgtcgaat ccactaataa
    42601 atatgagacg agtccaagga agtagtttcc ttgcttcttt tgaaaaagtc aacccgtcca
    42661 tgaaaggcat ttggatatca gtcagaatga tgtcgggctg taagtcgata attgaagaca
    42721 gtgccatctc accatctcca gcttcgccga cataagtgat tggataataa gaaccaagga
    42781 cagttatttg ttcccgcaag ttattgcgaa tgaggtattc gtcttcgatg ataaaaactt
    42841 tatacatgtt tgtttcctcc aattgttttg ggagatagag ggatcatcag tgtgacagtt
    42901 gttccttctc ggtatttgct gtcaatcgtg ataggttttt gatcatttcc gtaataaaga
    42961 aggagacgct tgtttacgtt gtaaagtccg tagccgatac tgaaatctga atcgatccct
    43021 ttggctaact ctttacgtat ttctgccagt ttttctgcag ggatgccaat cccgttgtct
    43081 ataactttga attggatcat ctcatcaatt ttgcatgtgg taatacgaat aaatcctttg
    43141 tgacggacgt acttcgttcc atggtataca gcattttcaa tcaatggctg caagatcatt
    43201 tttaaaatag aaacaggaaa aagtgtttct ggtatatcga tcttatagtc aagcatatgg
    43261 ccgtatctta tttttaagat gactagataa tcattgatat ggctaatttc tttttcaaga
    43321 ggaatccagt cttgtccttt gcttaaagac gtacgaaaaa agccggaaag tgcatgggtc
    43381 atagtcagag cttcttcaaa gcagtcttgt tcgatcaaag aaataatggc atccaaactg
    43441 ttataaataa aatggggagt aatctgagct tgcaatacac gaacttcact ttgtgctaaa
    43501 tggtattgtt tcaaagcgtt ctcccgtaaa gactggttta gatcttccgc cattttattc
    43561 atgctagcgg taagtgtttg taattccagt gtttccggca gggttaaacg aaagccaagg
    43621 ttccctgaag aaagttgttc tgacatctcg atcagctgat tgatcggttc ttggaccttt
    43681 ctctctaaaa agcgacgatt ctttttcgca tagaaaaaaa tggtactaag aactccaagt
    43741 tccactattg ttaatagaat gagcgagcgg acaagtgtcg tatttttttt gcttgctaag
    43801 tcgatctcca cacgaacaaa atcttgtaag atatctgaca gcagttgagt gacagaatct
    43861 acctgattga tgatctcttc attggtatcc ataaaatctt ttgccaagat attattgatc
    43921 agctcgtttt gataatcttc cagtgtatcg atcgtacgta gcgatacatc taacgtacta
    43981 atttctttaa gtgtatttgt attatttttg atctctcgaa tatcattcct aagttcttct
    44041 aggatatttt cctcattgaa ctcatctacg gttacttggc cagaaatcaa gtcccacata
    44101 ctttccaaaa ctttttgatc aaccttcaaa gcaattgcat ctgctttttg gatatttgcg
    44161 atcgtctgtt gataaatcaa aagctgttgc gaatataaca gtatgctcag tagcaaaggg
    44221 ataacagcaa atagaagcat ggctttgttt gcttgggcaa aaatctgttt tatgcttaaa
    44281 ttttttgtca tgatacttct ccagtcaata atttctggtt gggatgttag ctatattctg
    44341 ctgggaaaag acagtctcgg aaataaaaat atctttaggt aatgtttttc ctgtttgaat
    44401 atttccaaaa taacgagtaa cggtattggc tacataccag ccgacatcag gtttacactc
    44461 gactacacaa ttgacgattc ctgagcgtag cttctcgatc atatcttctt gtccatcaat
    44521 agttacaatg atcaaatctt ctccaggttt gattgatgtt tcttttatcg cagctaaagc
    44581 accgtgagtc atttcatcac tatggctgta taatacatcg atttcggata aatcatttgt
    44641 ttctaaatat cgtttcatct ggtcttttcc tttcatccga ataaaatcac cgacaattcg
    44701 gtgaacaata gtcatcggag gtttatggtc gatggttcca tcttttttta gggttggttt
    44761 acgattgatt gattccaaaa atccttcgga gcgtagcttg gtcggtgtgg agttctctga
    44821 accagcaagt tctaggattc tgatttcctg tttctttgaa tttgtgaagt gattgctgac
    44881 aaataaaccc gcccgatttc cttctgcttt gaaactgggg ccaatgtggg tgacgaaaag
    44941 ctcgggatca ttcgtttgaa tatgtctgtc gacaagtatg acagggatcc ctgctcgctt
    45001 tgcttctttt aatacagagt cccacccgga ttcttgcaaa ggagtaaaaa tgatcatatc
    45061 cactttatag gcaatgaacg tccgtatatc ttggatttgc cgttcctgat tcatgtagcc
    45121 atttcggtag agaacttggt aatcttcttt ttcaagcgca gtgataattg attcagtatg
    45181 ctttttccgc cagctactct ccgttcctga ttgcgaaaaa ccaacgacta gtgggtcagg
    45241 tgcttccttt gtattaggtg tacaccccat catttggtaa gcgattacaa caagggctaa
    45301 aaaaaataaa acacgtttca ctttaaatca ctccatctgg atttgttagt actatcttat
    45361 cagataaaag ggaaaatcaa agtcaaaagg aatcaatcga aaaaaatcca agaaacttaa
    45421 aaaaatccaa gacaaaaaaa gtacacctaa aaaatttaaa gtaaatccca taaaaaaatc
    45481 aatgttaacg ctttctttat tagctatact cggtgtatca attgaaatgg gggataaaaa
    45541 atgaaaaaca aatttagaaa gattgttggg actatcgcta ttttgaccgc aagcattcta
    45601 ttaagtgcgt gtggcaattc aggatctgca gatgatagta cggggtatgt aggaatcgct
    45661 atgccgacca aatcagcaga aagatggatc gctgatggga acaacatggt ctcggaatta
    45721 gaaaaactgg gttataaaac agatcttcaa tatggagaag ataaagtaga aaatcaagta
    45781 gcacaaatag aaaatatgat tacaaaaggg gtagatacac ttgtgattgc ttctatcgat
    45841 ggttcggctt taactgatgt gttagctaaa gcaaaagaag cagatatcaa ggtgatcgcc
    45901 tatgatcgtt tactgatgaa ttcagaaaat gtcgattatt acgcgacttt tgataatttt
    45961 ggagtaggtg tatcgcaagc agcttatatt gaagagcact taggattgaa agaaggaaaa
    46021 ggaccattca cgattgaatt atttggtggc tcgcctgacg acaacaacgc actaatcaat
    46081 tacaacggag tgatgtctgt tttacaacca tatatggaca acggtcagtt ggtcgtgcca
    46141 tctggacaga cgagtttcag tcaaatcgct acgcttcgct gggatggttc tacggcgcaa
    46201 gcaaggatgg ataatctgtt aagcgcaaat tatactgacc aaacattgga tgctgttttg
    46261 tcgccatatg atccaataag tttaggaatc atttcgtcat taaaaggcgt ggggtatggg
    46321 agtgaaagta aacctttacc tgtgattact ggacaagatg cgacagttgc tggcgttaaa
    46381 tcgatcattg ccggggaaca aacacaaacg atctttaaag acacacgtat cttagcaaaa
    46441 aatacgattg aaatgatcaa agcaatcagt gatggagaag aggttccggt caatgatact
    46501 gaaacttatg ataatggggt gaaaactgtc cctacgtatt tagcaaatac agtatctgtc
    46561 gataaagata attatcaagc agagctgatc gacaccgatt attataaaga atccgatctg
    46621 aaaaattaag aggaaccaaa caaggaacta aagagatcta agtcagagag aggataagaa
    46681 ttatggcgga ttgtatttta aaaatggcag atatcaccaa aaaattttct ggaatccgag
    46741 cgttagattg cgtgaatctg atgattgaac gtggggaaat ccatgcattg tgcggagaaa
    46801 acggagcggg aaaatcaaca ttgatgaatg tcttgagcgg cttgtatcca tatggctcct
    46861 atgacgggga aatcttttat gatggaaagc tgtgtcaatt taaagatttg aaagatagtg
    46921 aagaaaaagg catcgtcatc attcatcaag aattagcact aagtccttat ctttcaataa
    46981 aagagaatat ttttctcgga aatgaacaag cagaaagagg cgtgattgat tgggaactga
    47041 cagaaaagaa aacatatgaa ctgctgaata aagtcggttt gtctatcgac ccgaatcttc
    47101 tagtatccca gattggcgta gggcaacagc aactagttga aatagctaaa gcattatcaa
    47161 aatccgtccg tctgttgatt ttggatgagc ctaccgctgc attgaatgaa gaagaaagcg
    47221 cgaatttgct gagattgatc cgagagttga aagagcaagg tgtaacctcc atcattattt
    47281 cacataagct gaatgaaatc gtggatatcg cagatcgaat caccatttta cgggatggac
    47341 agacgattga gacactagaa aatgaaatga tcagcgaaga gcatattatt cgcggaatgg
    47401 tgggcagaga tttagttgat cgttatcctg aaagaaaacc ggatattgga gaagtttatt
    47461 ttaaagtaga agactggacg gtctatcacc ctcttgatca cgagcgaatc ataaacgacc
    47521 acatcaactt gcatttgaaa aaaggagaga ttgtaggaat agctggactg atgggagcgg
    47581 gcagaactga attcgctatg agtctatttg gacattctta tggaagcaag atatccggac
    47641 gtatattaaa ggaaggggca gagatatcag ttaagaccgt tccttcagcc attgcaaacg
    47701 gactcgctta tgtctcggag gaccgaaaat ctttaggact gaatctgctg atggatattc
    47761 gggaaaacgt gacgctgagc agtttggata aaataagtca aaaaggagta ttagataaag
    47821 aaaaagaagt aactgaggca gaaaatttcc ggaaaaagat gcgtataaaa acaaactcca
    47881 tccatcaaat tgtcagtagt cttagtggtg ggaatcaaca aaaagtcgtt ttatccaagt
    47941 ggctgatgac acaacccgat attctttttt tggatgagcc aacccgtggg atagatgttg
    48001 gggcaaaata tgagatctac acgattatcg aagaaatggc agcaaatggc aaagctgtct
    48061 gtcttatctc ttctgaactt cctgaaatat tagggatgtg tgatcgaatc tatacgatga
    48121 acgaaggacg tattacagga gaagtctcgc gagaagatgc agatcaggaa gtcttgatga
    48181 aattaatgac gaaagaagag gtgggttaag ttggaacagg caaaaaatct tacggataaa
    48241 aaagagaaaa aactaaattt gaaaagtaga gcagctgatc tttttggtcg ctacagcatg
    48301 gtgatcattt tagcgggact acttattgcg tttcaattga tgacggatgg tattttctgg
    48361 cgtccattga atataactaa ccttgttctg caaaatagcc atatattagt actagctgca
    48421 gggatgttat tagttgtgct cttaggtcat gtggatttgt cggtagggtc agtgatggcg
    48481 tttgttggtg caattgcggg catgatgatg gtcaatcgcc aaatgaaccc gtggatgaca
    48541 gttatcgcat gtcttgcagt aggcgggctg atcggcgcat ggcaaggttt ttgggtagct
    48601 tatgcaggga taccggcgtt tatcgtgact ttagcaggat tgctgatgtt tcgtggactg
    48661 acacaagttg ttctaggagg ccaatctcta gcaccttttc cagctgtttt ccaaaaaatg
    48721 tctacgggtt atttaccaga cttaagcgaa ggaagcagcc ttcacttctt gacattgatt
    48781 ttaggaatga tcatcacgat cgtgttagtt gttggtcaat ggcgaacaag aaatcgtcgg
    48841 aaaaatcatt tatttgaagt ggaatctcca acagctttct tggtaaaggc ggtatttaca
    48901 gcagtcgttg tactaggaat aacttatatc tttgcctctt atcaagggta tccgattatt
    48961 ttgatcattc ttggtttgat cgttgcagca tatgggtttt tgacaaataa aacagtagca
    49021 ggtcgacaga tttatgcgac gggcggaaat cgaaaagcgg ctgaactatc agggatcaaa
    49081 acaaagcgaa tcacgttctg ggtattcgtc aacatgggag tcatggcagc tttagctgga
    49141 ttagttttag ctgctcgatt gaatgcggca acacctcaag caggtacatc gatggaactg
    49201 gatgcaatgg cggcagtata ttttggtggt gcttcgactt ccggcggcat tggaacaatc
    49261 atgggtgcga ttgtcggtgg cttggtcatg ggtgtattga acaatggcat gtcgatttta
    49321 ggcgtcgggg tagactggca gcaggcaatc aaaggattga tcttgctttt agccgttgtg
    49381 ctagacttgt ataataagaa aaaacgaaca acgtaggaga acgccggtta ttcagaaaaa
    49441 agactgtgac aagacatttg tcacagtctt tttttcgcta aaagcgaaca ttcattgttt
    49501 cgtttgtata gactaaaaat aatatatgtt tttttaccat tcgtataaat tctctttttg
    49561 ctcttgattt ttttgcttaa ttgagttaaa ttacataggg cgctaaaaaa agccttgatt
    49621 aaattaataa tgttcgtgtt ttgctggatg tgaacatcag gaggataatg taatgaaagt
    49681 atttgattac gaagatattc aattaattcc taataaatgt atcgtaaata gccgttctga
    49741 atgtgataca acggtcacat taggtaaaca tacattcaaa atgccagtag tgcctgccaa
    49801 catgcagacg attattgatg aaacaatcgc agaattctta gcggaaaatg gttatttcta
    49861 tatcatgcat cggtttgatg aagcagctcg aattccgttt atcaaaaaaa tgaaaaagcg
    49921 tggattgatt acctcgatca gtgttggcgt caagaaagag gaatattcct ttatagagaa
    49981 gttggcagaa gaatcgctaa atcctgatta tatcactatc gacatcgcgc atggacatgc
    50041 taactcagtt attgatatga ttcagcatat taaaaaatat ttgcctgaaa cttttgtgat
    50101 tgccggtaat gttggtacac cggaagccgt ccgtgaattg gaaaatgcgg gcgcagatgc
    50161 aacaaaagtc gggattggtc caggaaaagt atgtattaca aaaatcaaga caggctttgg
    50221 gactggaggc tggcaactgg cagctcttag atggtgtgcg aaagcagctc gcaagccgat
    50281 catcgctgac ggtgggatca gaacacatgg cgatattgca aaatctgtcc gttttggtgc
    50341 gacaatggtc atgattggtt cattgtttgc cggtcacgag gaatctcctg gcgaaacaaa
    50401 agtcgaaaat ggaatcgtgt ataaagaata ctttggttct gcttcagaat tccaaaaagg
    50461 cgaaaaacga aatgttgaag gaaagaaaat ctggatccaa cacaaaggat ctttgaaaga
    50521 tacactagtg gaaatgcaac aagaccttca atcatctatt tcttatgctg gaggaagaga
    50581 tctggaagcc attcgaaaag ttgactatgt catcgtgaaa aattcgattt ttaatggaga
    50641 cacaatctaa aaaataaata aaatattagg atgttgtgaa aaagttgtcc tgcattaagt
    50701 aataagaaca gccaaacaaa aatagttctt tatatgtttt gtttggctgg aattattatc
    50761 gcagatacaa gcttttgaac atcattattc agaataaggg actgtgataa gatttttgtc
    50821 acagtccctt atgttttgca aaaaaagttt ttcttttttc tcgtaaattc ggtaaaatca
    50881 aaagaaaaaa tagacaatag aaaagagaag cttatgtggg aaaaagtaga gaaaaaacaa
    50941 ggaccgattt atcgtcaaat tatggaacag attatgagag aaatcgaaaa cggaaggatc
    51001 aatcctggaa gtcaacttga atcagaacga aaactagggc aactttttgg tgtgaatcgt
    51061 tccaccgttg tccatgcatt ggaagagctt agggagcttg gggtactaac gagcaaacgt
    51121 ggcagcggat ggtttgttaa tcagactgaa tgggggaaat tcgctgttcc aagaatcgat
    51181 tggcggcaaa tgatctctcc tcgttatgag cagacggact ggtatgagca aaaaataaga
    51241 gaagcaaagc agatccaaaa aacatcgttt cttgatttat acgctagcga gatgccagat
    51301 gagcttttgc cgaattttga gtttccttct tattctcttg aagaaatctt agcggaagaa
    51361 aaagaaatgg atcttttagg ctatactccg ttacgtaaca aaatcaaacg gtatttggaa
    51421 agtaaatttg aattggcatt ttcccccaac cagcttcttg tgactggtgg cggtcagcaa
    51481 gcaatctttt tgattttaca aacgattctt tcagctggag aggggatagc tgtagagtca
    51541 ccttcttttt tttaccgatt acctcttttc aaagcttcag gaacgcgttt atttgggata
    51601 ccgatggatg aagaagggat cgacttagaa aaactgaaag aatcgatcca taaaaataaa
    51661 ttgaaagcag tgcttatcaa cccgaatttt caaaacccta caggaaaagt gatgagtcaa
    51721 aaacgacgag aaaagctagt agaattgtgt cgacagtatc ggcttccaat tattgaagac
    51781 gatgtattta cggatctttc atttctaaac caagaagaag tccttcctat acgaacgatt
    51841 gatccggata atgtgttata cgtcggttcg ttatctaggg ttttaggaaa aacaacgaaa
    51901 atcggttggg tgataggacc tgagactttt atctcgcaac tagctcaagc gcaagaaatg
    51961 atggagtttt cgatgaatat ccttacacaa atcacagtgg ctaatgtatt taatgaagca
    52021 attgatttga aaatgcaaga tgtaagaaaa aagttgaaag aaaaaagccg actgataagc
    52081 gcttggggag attcacaatc gtttttcaag cttcattcaa ttttaggtgg ttactatgcc
    52141 tggattactt gggatgggaa aaaactagac cccgaactag cagaaaaagt agtagatatg
    52201 ggtcttggaa ttgcaccaag tctacttttt gggcaagata taaatggaat cagaatcaat
    52261 tacagtaggc tggatgaaaa ccaaataccg ttgttttatt caaagatgaa aattctagaa
    52321 aagtggttaa cagaataatt tcacaaagta aatagattaa agaatataac agcttggatt
    52381 taaatttcaa gctgtttctt ttttttcgga aaaagaatac gatataaaga aactaagaaa
    52441 aaaccattat ttgtacgtcc gttcacgatg tgtaaataaa ataaatgttt gcaacaaaac
    52501 tgtataacac caacgttttt aaatagaaac agatggttgt aagtttgatt agaaaataga
    52561 aaaaatgcag atgaaatata aaacgctttc cttattgtga ttttattcac taatgctgtt
    52621 aatatgcgga ttggcataat ttcctttcat cgtgaaaatt tgtgctttta gcttgtattt
    52681 ctcataaatc catgataatt tattaatgaa gacggttaaa tagtgataca gttttattca
    52741 ctgtttcaat cgcagaatga agagattttc ttatagatga gagaggaatg tgtgacaaat
    52801 ggcaaacaaa aaagcaccga tcgacttcca agcactgcta gatgaagtca atgcagattt
    52861 cccagtatat caagcacttg atcaagatgg aaacgtagtt aacaaagatt tagtacctga
    52921 tttatccgat gatgaactcg ttgaattaat gactcatatg gtatggtctc gtgtattaga
    52981 ccaacgttca acagcgctga accgccaagg acgcctaggc ttttttgcac caactgctgg
    53041 acaagaggca agtcagctgg ctagtgctta cgcatttgat aaagaagatg ttcttcttcc
    53101 aggttatcgg gatgtacctc aactagtaaa acatggttta cctttatctc aagctttttt
    53161 atggtctcga ggacatgctg caggtaattc ttatccggaa gaattgaaag ctctaccacc
    53221 acaaattatc atcggtgcac aatatgtaca agcagccggt gttgcattag gtttgaagaa
    53281 acgaaacaag aaaaatgtcg tcttcactta tactggtgat ggcggttcat cacaaggtga
    53341 tttttacgaa gcaatcaatt tcgctggcgc ttaccatgca aatgccgtat tttatatcca
    53401 aaacaacggg tatgcgatct ctacacctcg tgaaaaacaa actgcagcaa aaacacttgc
    53461 acaaaaagct gtcgcagctg ggattcctgg tattcaagtc gatggaatgg atccgcttgc
    53521 agtatatact gtatctaaaa tggcacggga atgggcgatc agtggaaatg gaccagtcct
    53581 aatcgaaaca ctgacttatc gttatggacc acatacacta tctggtgacg atccaacccg
    53641 ctaccgtaca aaagacacag atgacgattg gcagaaaaaa gatccattga tccgcttccg
    53701 taaatatttg acggaaaaag gactttggtc tgaagaaaaa gaagaacaag tcatcgaagc
    53761 aactaaagaa gaaatcaaag cagctattgc tgaagccgac aaagtaccaa aacaaaaggt
    53821 ttcagatttc ttgaaaaaca tgttcgaagt atctccacaa tcaatcaaag aacaaattgc
    53881 aatttatgaa gcgaaggagt cgaaataatc atggcacaaa aaacaatgat ccaagcaatc
    53941 acagatgcct tagcacttga actagaaaat gacgagaatg tcgttgtttt tggtgaagat
    54001 gttgggaaaa acggaggagt tttccgggca actgaaggat tgcaggaaaa gttcggagaa
    54061 gaccgagtat tcgatactcc tttagctgaa tctggaatcg caggattgag tttcggtctt
    54121 gcgttagaag gatttcgtcc agttcctgaa atccaattct ttggttttat tctcgaagca
    54181 atggacgaag tagtagctca aatggctcgt acaagatatc gaatgagcgg cactagaaat
    54241 ctacccatca caattcgttc gccatttggt ggcggtgttc atacaccaga attgcattcg
    54301 gataatttag aaggtttgat cgctcagtct cctggtattc gagtagtgat tccttccaac
    54361 ccttatgatg caaaaggact attgatttct tctattagaa gtaacgatcc ggttgttttc
    54421 ctagaacaca tgaaactata tcgctcattc cgagaagaag tgccggatga agcttacgaa
    54481 gttcctttgg ataaagcagc agttactcgt gagggaacag atgtatctat tatcacatac
    54541 ggagcaatgg tgcgtgaagc aatcaaagca gcagacaatc ttgcaaaaga aaatatatct
    54601 gttgaaatca ttgatcttcg tacagttgcg ccattagatg tagaaacgat cattcaatcc
    54661 gtggaaaaaa ctggtcgcgt agttgttgtc caagaagcac aacgacaagc gggcgttgcc
    54721 gcgcaagtag tttctgagat ctcagaacgc gctatccttt cattagaagc cccaatcgga
    54781 cgagtttctg cacctgatac agtcttccca tttggacaag cagaaaatgt ctggttgcca
    54841 aatgcaaaag atatcgaaga caaagtaaaa gaaatcgcgg aattttaaga aaagaaggga
    54901 cgaaacaaag tcatggctta tcaatttaaa ttgccggata tcggtgaagg aatcgcagaa
    54961 ggtgaaatcg tcaaatggtt cgtcaaacca ggagatacga tcaatgaaga tgatacatta
    55021 ctagaagttc aaaatgacaa atcagtagaa gaaatcccgt cacctgttac aggaacggtt
    55081 aaaaatgtga ttgttccaga aggtaccgta gcaaatgttg gggatgtatt agtagaaatc
    55141 gatgcaccag gacacgaaga caatgaaggg gacagtggtg tagccgcaga atcccaaact
    55201 ccagctaaac cggctgctga acccactgtt gatacagaat cagctggatc gtcaagcgaa
    55261 ggagtcttcc aatttaagtt accggatatc ggtgaaggaa tcgcagaagg tgaaatcgtc
    55321 aaatggttcg tcaaaccagg agatacgatc aatgaagatg atacattact agaagttcaa
    55381 aatgacaaat cagtagaaga aattccgtca cctgtcacag ggacagttaa aaacgtgatt
    55441 gttccagaag gcactgtagc aaatgttggg gatgtattag tagaaatcga tgctcctgga
    55501 cacaatagtg caccgtcaac tagtgcgcca tcagctgaag caccaaaaga aaaagtggaa
    55561 acatcaggtt cagcaagtgt cgttgaagct gccgatccaa ataaacgtgt attggctatg
    55621 ccatctgttc gtcaatttgc ccgtgaaaaa gatgtagata tctcacaagt aactgcaact
    55681 ggaaaaggtg gcagagtaac aaaagaggac atcgaaaact tcttggcagg tggtccaagc
    55741 agtgcaccag cgaaatcaga agcgccagaa gcggcagcac cgaaagaagc agctcctgca
    55801 gcagaaagca aaccagctgc accagcaaaa ccattcaagt ccaacttagg agacttggaa
    55861 gaacgtgtag caatgacgcc aacacgtaaa gctatcgcta aagcaatggt caacagtaag
    55921 catacagctc cacacgtgac attgcatgat gaagtagaag tgtctaaact atgggataat
    55981 cggaaacgat ttaaagaagt tgctgctgca aatggtacga aacttacttt cttgccatat
    56041 gtcgtgaaag ctttaacagc gactgttaag aaatacccag tattgaatgc atctatcgac
    56101 gatgcaaacc aagaaatcgt ttataaacat tactacaata tcggtattgc aactgatacg
    56161 gatcacggac tatatgtacc aaatgtgaaa gatgcagacc gcaaaggaat gtttgctatt
    56221 gcagacgaaa tcaatgagaa agcaaaatta gctcatgacg gcaaactatc tgcagaagat
    56281 atgcgcaatg gaacaattac gatcagcaat atcggatctg taggtggcgg ttggtttaca
    56341 ccagtcatca actatccaga agtagctatt ttaggtgtag gaacgattgc acaacaacca
    56401 atcgtaaatg ctgaaggcga aatcgttgtc ggtcgtgtga tgaaactgtc attaagtttc
    56461 gatcaccgta tcgtagatgg agcaactgcg caacaagcaa tgaataatat caaacgtctt
    56521 ttagctgatc cagagctatt aatgatggaa ggatgataaa caaatggtag ttggagattt
    56581 tgctgtagaa ttagatacag ttgtaattgg tgcgggacct ggtggctatg tagctgccat
    56641 ccgcgctgct gaaatgggac aaaaagtagc aatcattgaa agagagtata ttggcggtgt
    56701 ttgtttgaat gtcggctgta ttccttcaaa agcactgata gctgcaggac accattatca
    56761 agagtcacta gattctgaaa tgtttggagt aactagtgaa aacgtcaaac tagattttgc
    56821 aaaaacacaa gaatggaaag aaaacaaagt tgtccatacg ttgacttctg gtgttggttt
    56881 cctattaaaa aaacataagg ttgaaacaat cgaaggagaa gcgttcttcg tagatgatca
    56941 cacactacgc gtcattcatc cagattcagc tcaaacttat tcgttcaata acgcaatcat
    57001 cgcaacaggt tcccgtccaa tcgagattcc aggatttaaa ttcggaggac gtgtgttaga
    57061 ttctactggc ggattagcat tgaaagaagt acctaagaaa tttgtgatca tcggtggcgg
    57121 tgtgattggt gccgaattgg gcggagcgta cgcaaactta ggagcagaag taacgattct
    57181 tgaaggctca ccacaaattt taccaactta cgaaaaagat ctagtaaaat tagtagaaga
    57241 tgatttcaag aaaaaaggcg taacagtcgt aacgaatgca atggcgaaag aagcagtcga
    57301 taacggtgac agcgtgacag tgaaatatgc agtggacggg aaagaagaat ccgttactgc
    57361 tgattatgtg atggtcactg taggtcgtcg tccgaataca gatgatatgg gcttagaaca
    57421 agcgggtgtc gaagtaggag aacgaggatt gatcacggtg gataaacaag gacgtacgaa
    57481 cgtacctaac atctatgcca tcggtgatat cgttccaggg gcggcattag cacacaaagc
    57541 cagttatgaa gcaaaaatcg ctgctgaagc aatctctggc aaaaaagtag ctgtagatta
    57601 taaagcaatg ccggcagtag ccttcactga tcctgaattg gcttctgtag gtatgacgat
    57661 caaagaagca aaagatgctg gtatcgaagc aaaagcatat aaattcccat tttcagggaa
    57721 cggtcgggca ttatcgcttg gtaaaacaga aggattcatc cgtctagtta cgacgatcga
    57781 agataacgtg cttattggcg cgcaaatcgg tggagtcggc gcaagtgata tggtctctga
    57841 attagcttta gcaatcgaat caggaatgaa tgctgaagat atcgccttga cgattcatcc
    57901 tcatccatct cttggcgaaa tcgtcatgga tgcttcggaa ttagcattag gtttaccaat
    57961 ccatatttga tttgaataat acaggcgaaa cgcaactgaa aaatcagttg cgttttgttt
    58021 tttagaatta tcaataatat tattatgtaa acttaaaaat atgacaactt cttggtttat
    58081 taccacttcc tatgctacac tgaagaggaa tatgaaatag gagagttgaa gatgcaagaa
    58141 tatcagtatc cacttgattt agattggaca acagacgaaa tggtcattgt aatgaagatg
    58201 tgggaagatc tggaacgtgc gaatgaaaaa gggataaaca atgaagcttt tttgaagact
    58261 tatcagcagt ttaaaactgt tgtcaaatcc attggtgagg aaaaacgatt gggacgggaa
    58321 tttgaaaaag cttccggtta ttctttatat cgtacagtgc agcaagcaaa aaaaaatcca
    58381 aacaaaactt tgagaatgaa cgggtgagat aaatggaaca gatgattgat catatcaagt
    58441 cctggcttga agaagctggt gagaaaatca aatcttctca acaaacatta acaatctcgc
    58501 aaaaatcaaa tcgaacggat ctagtaacaa atgtcgatcg aaacatccag aattttctaa
    58561 tcgaaaaaat ccattccttt gatccagagg cgaaaatatt ggcagaagaa gaagggtgta
    58621 atcaattatc ggatctcgat ggaagagtct ttatcatcga tcctattgat ggcaccttga
    58681 attttgtgat tgaaggggaa aatttttgta ttatgctcgc tgtttatgaa tccggcgttg
    58741 gaaagcttgg tttcatttat gacgtgatga gaaatgaact tttttggggt ggaaaaggaa
    58801 taggcgtgta taaaaatgat gagccattgc ctagaccagc aaataaatcc ttatcggaag
    58861 gcttacttgg tgtgaatagt tatcttttcg gtcataataa atatggaatc cgagaaattg
    58921 cggaaagcag catggggaca cgtatgtacg gatgcgctgg tcttgaacta gtggcaatct
    58981 tgaaaggaaa ccacattgct tatatttcaa accttagccc ttgggattat gcagcgggaa
    59041 atgtcttatt ggaagagttt ggtatggtaa caagcggtct ttttggtact cctttgacat
    59101 ttgaaaaaag ggaatactat ttagcaggga cttcttgtgc atatgcagaa atcgcagacc
    59161 ttttgaaaaa agaaggctga aaaaaaggta gaaatcattc acaaaaatga taaaaacgag
    59221 caaaagatta attttctcga aaaaatgaaa acattttact atatttcatg aaaaaagtct
    59281 gctataatta gcagtcgagt aaaatttgcc ctgtcataaa gaaccaataa aaaaggagtt
    59341 agttacattg aattatagaa acgatatccg taacgtcgca attattgccc acgttgacca
    59401 cggaaaaaca acattagttg atgaactatt aaaacaatca gatacattag acgcacatac
    59461 gcaattgcaa gaacgtgcga tggactcaaa tgcgctagaa aaagaacgtg gtatcacgat
    59521 tttagccaaa aacacagctg tagactataa aggtattcgc gtaaacatca tggatactcc
    59581 aggacacgcg gacttcggtg gcgaagtaga acggatcatg aaaatggttg acggcgttgt
    59641 gttggtcgta gatgcgtatg aaggaacaat gcctcaaaca cgttttgtat tgaaaaaagc
    59701 actggaacaa catatcacac caatcgttgt cgtaaacaag attgacaaac cttctgctcg
    59761 tcctgagcat gtcgtagatg aagttcttga actattcatc gaattaggag cagatgatga
    59821 tcagttagac tttcctgtga tttatgcatc tgcactaaat ggtacgtcaa gcttgtcaga
    59881 tgatccagca gatcaagaac caaccatggc acctattttt gatacaatta ttgaaaaaat
    59941 ccctgctcct gtcgataaca gcgacgaacc attacaattc caagtttcat tacttgacta
    60001 caacgattat gtaggtcgta tcggaatcgg acgtgtattt agaggaacaa tcaaagtagg
    60061 agaccaagta gcattaatta aattagacgg aacagttaag aaattccgtg taacgaaatt
    60121 atttggtttc tttggactaa aacgtctaga gattcaagaa gcaaaagctg gagacttgat
    60181 tgctgtctca ggtatggaag atatctttgt cggtgaaaca gttactcctg tggatcatca
    60241 agatgcgtta cctatccttc acattgacga accaacattg caaatgactt tcttagttaa
    60301 taattcacca tttgccggtc gagaaggtaa attcgtaacc gctcgtaaga ttgaagaacg
    60361 tttgatggca gaacttcaaa cagatgtatc cttacgagtt gaaccaacaa actctccaga
    60421 tgcatggaca gtttctggtc gtggagaatt gcatttgtct atcttgattg aaaacatgcg
    60481 ccgtgaggga tacgaattgc aagtatctcg tccagaagtt attgaaaaag aaatcgacgg
    60541 tgtaaaatgc gaaccgtttg aacgcgtgca aatcgataca ccagaagaat atatgggtag
    60601 tgttatcgag tccttgagct tacgtaaagg tgaaatgcaa gatatggtcc atacaggaaa
    60661 cggacaaatc cgtttgacat tcttaacacc tgcccgcggt ttgattggtt attcaactga
    60721 attcctttca atgacacgtg gttacggaat catgaaccat acttttgacc aatatctgcc
    60781 aatgcttcct ggtcaaattg gcggacgtca ccaaggggcg cttgtttcga tcgatacagg
    60841 aaaagcaacg acttattcta tcatgagtat cgaagaacgc ggtacagtct tcgtagaacc
    60901 aggtacagaa gtatatgaag gaatgattat cggggaaaac agccgtgata atgatttgac
    60961 tgtcaacatc acgaaagcaa aacaaatgac aaacgtacgg tctgctacta aagaccaaac
    61021 atctgtcatc aaaaaaccaa aacaattgac gttggaagaa tctttggaat tcttgaatga
    61081 tgatgaatat tgcgaagtaa caccagaaag cattcgcttg agaaaacaaa ttttagagaa
    61141 aaatgctcgt gaaaaagcat ctaaaaagaa aaaataatct gtaaaaggat gaagagagaa
    61201 accagcttgt tataaaaaat agctggtttc tttttttgtc caactctttt ctgtggagaa
    61261 gtcatctttg tcaatggaca taaattcaga aaatacgccg aaagacgttt aaaagacttt
    61321 taaaaaactt atttctagag agtttttgtt tcataataag ataatgtttc acgaaaggag
    61381 atcaacttgt ccttcgaaga aaatcgaagt aaaatggcaa gagaaaggag cgggaagatg
    61441 aacgacttat taaaacaata ctttggctat gatgagttta gacctggtca aaaagaaatt
    61501 atccagaaag tcattgatca ggaaaatgta ttaggaatca tgcctaccgg aagtgggaaa
    61561 tcaatctgtt accagcttcc agctttacta ttagatgggc ttactgtggt tgtgtcccct
    61621 ttaatctctt tgatgaagga tcaggtggac gcggcgaatc aattaggtat acccgccact
    61681 tttatcaata gctcgttaga tggttacgaa acagctagac gcttccaaga aatcgataga
    61741 cagcagtacc gtttacttta tatcgctcct gaacgtttta tcatgccgga ttttatccaa
    61801 gcaatgaagc gatggaacgt ccgtatgatc gctattgacg aggcacattg tatctctcag
    61861 tgggggcatg atttccgccc aagttattta cagatggcga accagctaga tcaactgcca
    61921 aatcgtcctg taattgttgc tttgacagct acagcgactg tgcaagtagc tgctgacata
    61981 aaaagacttt taaaaattcc agaaaacaac catattcaga ctggttttga acgagaaaat
    62041 ctacgatttc aagtaattaa agatcaaaaa aaagaacaat atctaatcga atatttaaag
    62101 atcaacaaaa atcaatctgg tatcatttat gcagcaacga gaaaagaagt agaccgtctc
    62161 tatcatttgc ttaaaaaatt cgatttttcc gtgggtagat atcatggtgg tctaaatgag
    62221 aatgagcgaa cagaaatgca agaagccttt ttatacgatc gcttgcagct gattgtagcg
    62281 acaaatgcat ttggtatggg tataaataaa agcaatgtcc gttttgttat ccattatcaa
    62341 attcctggct ctctagaagc atactatcag gaagctggtc gggcgggaag agatggtctt
    62401 tccagtgaag ccatcctgct tttctctcct caagatatcc aagtccaaaa gttttttatc
    62461 cagcagtccc aaagagaaga agggcaaaag caaaaagaat atgaaaaatt aaaagcgatg
    62521 acagaatacg tgtatattga gtcctgtctt cagcaataca tcctaaacta ttttggcgag
    62581 acaagcagtc cgtgcaaccg ttgtggtaac tgtttagatg acagagaaat agtagaagta
    62641 acgacagaag cacagatggt tctttcttgc ttaaaacgta tgggtgagaa ctatggtaaa
    62701 caaatgctga tgaaagtctt agcaggatcg aaagaacaaa agctgcaagc tttaggtttt
    62761 ggacatttat ctacctatgg actgatgaaa aaccaatcgc aaaaagaaac gatgcagtta
    62821 attgaatatt tgatatcgaa tggctatctt ctgaccatca atggtgagta tcctgttttg
    62881 aaagtaacag aaagagggat acaagtattg aaaggacaag aatctgttta tcgaaaggaa
    62941 cccaagaaag tccagcagct atcggatgaa gaaaccgata cactatttga agtgctgcgg
    63001 gaactgcgaa cggatctagc atcagaagca ggcgttcccc cgtatgtcgt attttcagat
    63061 tccacattga aagaaatgag ccgaatacgt ccatcaagca gattggagat gcttcagata
    63121 aaaggagttg gccaaagcaa gcttgataaa tatggcgaag cctttttatc tcggataaaa
    63181 aatgcggact ctaacgttct ggataagtag aaagaacgaa tacgtaaaaa tgaataaaaa
    63241 caactggtta aatctgtttt ttttgctcct tatgaaaatc aaatatgtta taattaagga
    63301 attagaatgg gaggtaagca cagatggatt ctttgtcgaa aaaacttgcg atccaagtat
    63361 taaatgatga agcagaacgg atcaaacgat taatccgaaa tcaaaacaac agtctctgta
    63421 tctcacaatg caaagccttc gaagaagtgg tagatacaca gatgtacggc ttttcccggc
    63481 aagtcggttt tgctgtccgt ataggagtaa tcggaaagaa cgagggccag ctgatgttaa
    63541 gtgagctaga acgagaattg aatcagttat atagtgaggt ttaccaagaa atttatgata
    63601 aaaaagaagt tggcaaggag gaatagactt gcctaagaaa gtgaaaaaaa ggcatctatt
    63661 agactatagc atcttgattc cctatttaat tttatgtgta ttgggattga tcatggtata
    63721 tagttctacg tcgtatctat tattagaaaa cggacaaaat ccgtccgcat cagtcattaa
    63781 ccagagtatt ttttgggtgc ttagcttgat tgctatcgca ctactttata aaatgaaaac
    63841 agatgttttg aaaaaccagc gtttgatcat ggcagccatc gcagttttga cgattttgct
    63901 gctgattgtt gtgttttttg gtaaggagat caatggggcg aaaggttggc tgcaaattgc
    63961 aggattctcg atccaacctg ccgaatacct aaaaatcatt tctatctggt acctgtctct
    64021 gactttatct aaaagacaaa acagtgtaca aaaaaatttt cttgggactg tgaaacgtcc
    64081 acttgcaatg gttatcggac ttactgcatt agtcgctatt cttcccgatt ttggtaatgc
    64141 ggcagttatc tttttgatta ttttggttct gcttttagcc agcggagtca attatgtgta
    64201 tactttgatt gtaggagtag gaggcttttg tctaagtact tttacgatct ggctgatcaa
    64261 tatcacaaac ggaaagatct tacccggacg tttacagtat atctataatc gatttgctat
    64321 ttaccaaaat cctttctcag acgaattaaa taaagggcat caactagtca acggctacta
    64381 tgccatgttc aacggaggac tatttggcag gggattaggt aacagtatcc agaaaaaagg
    64441 atttttacaa gaagcacaaa ccgattttat ttatgcaatc gtagtggaag aactgggagt
    64501 gattatgggg attttgatat tagcgttgct attctttatg attgtgcgca ttgtccttgt
    64561 aggtatccgt tcaaaagatc cgttcaattc gcttttgtgc attgggatcg gcgcaatgtt
    64621 tttgatccaa gtatttgtca atcttggagg aattactggc gttattcctt tgacagggat
    64681 cacgttccca tttttaagtc aaggaggctc cagtttgctg atgctttcga tttgtgtcgg
    64741 ctttgtcctc aacatcagtg cggatgaaaa acgcaagagc ttaggtttat aataaaaaaa
    64801 gatgtaggag tggttcgatg aaaaaagttt tagttgctaa caggggtgaa atcgctgtac
    64861 gtgtatttcg tgcttgtaca gaattaggaa ttaaaactgt tggaatctat gcagaagaag
    64921 atgaatattc cgtacaccgt ttcaaagcgg atgaagcata tttagtagga caaggaaaaa
    64981 aaccgattga tgcgtatcta gatattgaag ggatcatttc tatcgcgaaa gaatgcggag
    65041 ctgacgctat tcatccgggt tacggtcttt tgtcggaaaa cttgaatttt gcgcaacggt
    65101 gcgaagaaga aggcatcatt tttgttggac ccaaactgca tcatttagat atttttggag
    65161 ataaaatcaa agcaaaagct gcagctatcg aagcggggat tgcttcgatt ccaggcacag
    65221 atggaccaat cgcttctata gacgatgcct tggagtttgc gaaacaatac ggttatccga
    65281 ttatgatcaa ggcagctcta ggcggcggcg gacgcggcat gcgtgtagct cacgatgaaa
    65341 agagtgcaag agagggctac gaacgtgcga aaagcgaagc aaaagcagcc tttggtagtg
    65401 atgaagtgta tgttgaaaaa tacatcgcta atcctaagca tatcgaagta cagattctcg
    65461 gggatacgca tggaaacgtc atccatcttt ttgaaagaga ttgttctgtt cagcgccgtc
    65521 accaaaaagt agtagaagta gcgccttgtg tctctatgaa tgaacagcaa cgtcaaaaaa
    65581 tctgccaggc agctgtccaa ttgatgaaat atgtcgggta tgtcaacgca ggtacagtag
    65641 aatttcttgt tgaaggagat gatttttact ttatcgaagt caaccctcgc gtccaagtgg
    65701 aacatacgat cacagaaatg attacagata ttgatatcgt aacaacgcaa ctgttgattg
    65761 ctcaaggttt ggatctgcat aaagaaatcg gattgcctca gcaagaagga attaaattaa
    65821 atggttcagc cattcaatgc cgaatcacta cagaagaccc gttgaataac tttttaccag
    65881 acacagggaa aatcgatacg tatcgttcac ctggtggttt tggcgtacgt ctggatgtcg
    65941 gaaatgctta cgcgggctat gtagtgacac cgtattttga ttcattgcta gtaaaagtat
    66001 gtacgcatgg tgctaccttt gaaacagcca ttcagaaaat ggaacgttgc ttgaaagaat
    66061 ttagaatccg tggagtgaaa acaaacattc catttatgct caatgtcatc acacatcctg
    66121 aattccaatc aggaaatgca aaaacaacct tcattgatag tacagcaaca ttatttgaat
    66181 ttcctcgact acgagaccgc ggaaacaaaa cgatgaaata tatcggtgaa attactgtga
    66241 atggttttcc aggtatcgaa agtggcgaaa aaccatttta tgaagaacca cgtatgccta
    66301 aagatctaat cacaagaact gattacgtaa cggctaagaa tgtattagat gcgaagggtg
    66361 ccgatgcttt ggtcgaatgg atcaaaggac aggaaaattt gttgttgacg gatacgacat
    66421 ttagagacgc ccatcaaagt ctgctagcaa ctcgtgttcg gacaaaggat ttcaagcaga
    66481 ttgcacgttt gactggagaa ggtcttcctg aactcttttc aagtgagatg tgggggggag
    66541 caacatttga tgtggcttac cgctttttga atgaagatcc ttggcaacgg ttgagaaaaa
    66601 ttcgttcttt aatgcctaat acgttgctgc aaatgctttt cagaggttca aatgccgttg
    66661 ggtattctaa ttatcctgac aatgtattgg tggaatttgt caaagaagca gcagcgcaag
    66721 gaatcgatgt atttcgaatc tttgatagtt tgaactggac tccacaaatg gaaaaaagta
    66781 tccaagctgt acgggatact ggtaaaatcg cagaggcagc tatttgctat actggcgata
    66841 tcaacgatcc aagtcgggca aaatacaatg ttcagtatta taaagacatg gcaaaggaac
    66901 tagaacaact gggtgcgcat atcattgcaa tcaaagatat ggctgggctg cttaaaccgc
    66961 aggcagctta tcgtttgatt agtgaattga aagaaacgac agatctgcca atccatctgc
    67021 atactcatga taccagcggg aatggaatca tcacttattc tgctgcttca aaagcaggag
    67081 tagacatcgt tgacgtggca atgagtgcaa tgagtgggaa tactagtcag cctagcatga
    67141 gcagcttgta ttatgcactg gtcaatggtc cacggctgcc agaaatcacg attgaaaatg
    67201 cacagaaatt aaaccattat tgggaagatg tgcgcatgta ttacaaaccg tttgaaaacg
    67261 gcttgaatgc tcctgaaaca gaagtatata tgcatgaaat gccaggagga cagtattcga
    67321 atctacagca acaggcaaaa gctgtcggat taggtcatcg ctgggatgaa atcaaacaaa
    67381 tgtatcatac tgtcaacttg atgtttggcg atatcgttaa agtaactcca tcatctaaag
    67441 tagttggaga tatggcacta tttatggtcc aaaatgatct tacagaagaa gatatttatg
    67501 aaaaaggcga aacactaagt ttccctgaat ctgttgtgac tttcttccaa ggagaactag
    67561 ggcaacccgt tggcggattt cctaaaaaat tgcaaaagat tattcttaaa ggcagacctg
    67621 cattgaatga acgtccaggt cttttggctg aatcagtcga cttcaacgaa gtaaaaaaag
    67681 aattggcaga aaaaattggc tatgaaccaa aacaagacga agtattaagc tatttgatgt
    67741 atccgcaagt ctttttggac tatcaaaaag catataatca atttggcgat gttacattac
    67801 tggacacacc aacatttttc caaggtatac gtttgggtga aacaatcaat gtacaaatcg
    67861 aacgcggaaa aacgcttatc attcgtttag atgaaatcgg agaacccgat atcgaaggga
    67921 atcgcgtact gttcttcaat ttgaatggac aaagacgtga aatcactatc aaagataatt
    67981 cgatcatcag tacggtccaa acccgattga aagctgaacc gacaaaccga gaacaaattg
    68041 gagcaacgat gtctggttct gttttggatg tattagtgaa aaaaggcgat aacgttaaaa
    68101 aaggtgatac gttgatgatt actgaagcga tgaaaatgga aacggccatc gaagcacgtt
    68161 ttgacggaga agtcgcacat gtatatgtgt cttcaggaga cactatttcg tctggtgacc
    68221 ttttgattga agtgactgag aaataataag cgaaaggtgg ggaaacatga aaagactggg
    68281 tttgttttgg ctgattctgc tattagttat tgtggcattc tacatggaac cagtcttgtt
    68341 tccgccaaaa cttgaagtga aggaaaactc tgatcaacaa atatcaaacc aaatgacaac
    68401 gtggaaatac caggagctta aagcaactgg tttttctact tatatcggaa aaccgatcga
    68461 tcagctggaa aaggattttg gccaagccta tgatcgatta actagcggat ttggttttga
    68521 aacaagatat tatgaaaata aagctagtca tctcacattt gaagcgaaca tccaagacag
    68581 cagagtcaca actgttaaag tactgcaaac agggcctgct gatatcgcgc cgttttattt
    68641 agggatgacg atgcaagatc ttacgaaaat cactactatc tatactaatt tcacttttga
    68701 gtaccatgat acagaagttg gaatcgaact aatggaagag gatatgaatt atcgtccgct
    68761 aattgctttt gataacgaca cttttgctat tttatttttt gatcaaacaa caaacggcct
    68821 attttcagtt gtgtatttag ataaagatag tcttttgaaa ctattgccat atcaagtgtt
    68881 tggtgaaggc cttccacatt atcaagcaga taaatcggct gattgggaat caatcaatcg
    68941 cgttaaagaa cgaaaaagta cgactttatt aaatatgctt agaagagaag acgaacttcc
    69001 catatacaat caaacacttg cctttcaaaa ccacagtcag ctgctactac atgatttttt
    69061 gacaaggcct gaggaaatat tgtcagagga acgtatggct gattggcaaa aatcgctagg
    69121 cagtgctcaa acgacagtga aattctcact gacgaatgat gaattggaga agttaatcag
    69181 taaacaaaaa ttagaaaata ctagtgggct atttattcat ccggcgattg acgctacttt
    69241 ttcgttttta tattggtaca gcgatcctta caaccatgac cgtttcatgg acccgggaac
    69301 ggattctgta ggcattgctt tttctaaaga aaatatgtta gtcttattgc aagaagaaaa
    69361 cggagaaagt agtgatagca gtgatcgtta acgaagagct gtttcaactg gaggatcaat
    69421 gcctccaagt cgctgaaatg ataaaaaaga gtaaaacgat ggcttcttat cttgaacata
    69481 aacagtcaat ggaagaatct gcaaaagttt gccagttgaa agaagcgttc ttgtctaaga
    69541 aagaagcatt tgaaaatgta gcggcatatg gtgagtatgc acctgattat cgtgaaaaaa
    69601 aacgagttgt tcgaaaagca aaaagacagc ttgatatgga tgagcatgtt gcagctttcc
    69661 gatatagtga aacacagctg caaagtctgt tggatcaaat tgtccaagta cttgccgaag
    69721 aagtttctaa tgaaatcaaa gtagatgcag gtagcccgtt ttttgaaacg aaaaaacact
    69781 ttggatgcgg aggtaattgt catgcaatta gacaatagtg aatttgaaat aacaaaacga
    69841 cgcgggctga tcgtatgggt ctacagctta aaacaattaa agaatctgaa aaagttcggc
    69901 ttgttgcatt atgtctcaag aagaatgaaa tatgtcgtgc tttacttaaa tgaagagaca
    69961 ttggaagaaa cagaagaacg aatccgtaaa ttacattttg ttcgaaacgt agaacgttct
    70021 taccgaccgg atatcgaaat gaattttgcc gaaaaaatag gaaacaaaac agtattaaaa
    70081 gacgaaggtt tcgaaattga agagctaaat acagaaatcc gattagctga acatctttaa
    70141 aaatctctta tgagcagttt ttaaataaaa aataacttgg ataacgatcg atttttgatc
    70201 attgtccaag tatttttttg gtatactagt accagtacca tttctaagga gtgagtagat
    70261 gcggataata tcaggaaatt atggcgggcg ccgtttaaaa agtttagcag gggcgaacac
    70321 cagaccaact acagataaag taaaagaatc catattcaat atgattggac catattttga
    70381 tggagagaca gtattggatc tgtttgcagg aagcggtggt ttagctatcg aagcagtatc
    70441 tagaggctgc agtcatgcgg tatgcgtaga caaaaattac caagcgctaa aaatcatcaa
    70501 agaaaacatc gaaattacga aagaaccaga gaaatttacc acaataaaac ttgatgcgga
    70561 caaagcaatc gaacggctag cagcggagaa acaacaattc gactacttgt ttcttgatcc
    70621 gccatatgca aaacaaaaaa taatcgatca aatccataaa atggaagaac tgggactttt
    70681 ttctaaaacc gcagttatcg tatgcgaaac ggataaaaca gtcgaattgc cagaaactgt
    70741 ggctggattc catcaaataa gaaaacagat ttatggcata acggccgtaa ctatctatag
    70801 aaaagaggga aatcaatgaa acgtgcatta ttcccaggaa gttttgatcc ttttacaaaa
    70861 ggtcacttag atacggtgga acgtgcggct aaattattcg atgaggtagt aattggtgtt
    70921 tttatcaata cttcaaagaa aagtttgttt cccccagaag aaagaatgac attgatcaca
    70981 aaagctgttt ctcatcttcc taatgtaaaa gtcatgcatc aagaaaatca attgacggta
    71041 gaaacagcaa aagagattgg tgcagatgcg ttgatcagag gcatacgaag tatcaaagac
    71101 tttgaatatg aacgagagat tgctcagatg aatcatcatc tatatgatga attggaaact
    71161 gtcttcttat tagcaaaacc agaatatagt cacgtgagtt ccagtatttt aaaagaagta
    71221 ctgcactttg gcggagatgt gagcagctac ttaccgccag tgatcaacga agctttagct
    71281 ggaaagcgtg aatcaaatga aagctaaaca atggttgaaa acagtactga ttatcatatt
    71341 tggaatgttg atcattggat ttgtagtagt tcccttgccg tattatgtag aatcaccggg
    71401 agccacgatc gatctgaaag aactaattac ggtcaatgat aaggaagata aaggttctgg
    71461 gtctttctct cttacctctg ttggtatcag gcaagctaca ggtttcttag ctttaaaagc
    71521 taaaatgacc ccttttgaag aattgatcag tgaagaagaa ttgactggtg gggcgacaaa
    71581 tgaagaatat aatcaaatgc aacagtatta tatggattct tcccaaaatg cagcaatcga
    71641 acaagcgtta aaacttgcag acaagcctta taaaatggaa ttcaaaggcg tatatgtcat
    71701 gagtgtagag aaaacatcga atttctttgg caagctttca gttggagata cagtaacaaa
    71761 agtagacggg aaaagctttc aaagtacaga agcatttatg gattatgtaa aaagccaaaa
    71821 agttggacaa acaatcgaaa tcagttattt gcataatggt gaagaaaaaa cagcttctgg
    71881 tgatctgatc gagttaccaa cagataaaaa ggcaggaata ggtatcacgc ttactgacca
    71941 taccgagatc gaatcagata cagatgttcg tattaattct ggctctatcg gtgggccatc
    72001 agcgggattg atgttcactt tagaaatcta tgaacaagtc actggtaaaa acttgcgaca
    72061 tggtaagcaa attgcaggaa ctggaacgat caacagtgaa ggagaagttg gccgaatcgg
    72121 aggaatcgat aaaaaagtgg caagtgcgga taaagctggc gtagagattt tctttgcacc
    72181 agatgatacg atttcagacg atgttaagaa agaatatcct gaaataaaaa ccaactacga
    72241 agaagcaaaa gctgccgcta aaaaattgaa ttcctcaatg aaaatcgtac cagtaaaaac
    72301 agtacaagaa gcgcttgact atctagagaa aatgaaatga aactaaataa ttaaagaggg
    72361 gttgtgacac gagttctgtc acaacccctc tttttcgtca aaaatcttca cgataaaaat
    72421 aaaaacaaaa gaaaaaattg acgtaattag atgttgaagg gacgattttg ccagagctca
    72481 ctgtttcttc aattttaaat gaaaggagta atgaatgatt tttactggtc attctttgaa
    72541 aaaagaacaa tgatagaaaa aatcagcaag aaaatactag cttcacccaa attgatcatt
    72601 tggatgtcta tgatagttct tatcttttgt atcgcaagtg gttttttatt atcaaatttg
    72661 ctattttata gagaaacaga agagactgct gcaaaagaaa tactttcttc gtttagttcc
    72721 agtggagaag atccatcagc tgaaggaaac gatgtagaag aggatgctcg tccaaaggtc
    72781 atgtacaccg atatcaaagg aagtgtgaag gagcctggta tctattcctt ttcttcagaa
    72841 gaacgggtct atgatgtact aaagcgtgca ggtggattat tagaagaagc ggatagcgat
    72901 cgaatcaact tttctgcgaa gatagaagat cagcaagtcc tatatatccc tgctgtaggt
    72961 gaagagctgc cagaacatct tgatcagttg gcatcctcgg aaggaaaaca gcctacagca
    73021 gattccgagc ctcccaaaat aaatatcaat acagccactc catcagaact tcagcagatt
    73081 cctgggatcg gatcagtcaa agcgcaagaa atcattcgat ttagagaaga aaacggatcc
    73141 ttccagaaag tggaagacct gcaggagatt tctggtatcg gtgaaaagac agttgaaaaa
    73201 ctaaaaaact tcgttacaat taaatagaat gattcaaaat cgactggcac gagcaataaa
    73261 tggacgctaa tggatactaa gaataatcca attctaagag cttggcagga cgaggaggta
    73321 gtatttttgg ataggaaata gcttgattgt tcgcaaagaa acattaaaaa aaccaatgct
    73381 gaatgtctat ctccaaaata aaatatctgg gattcatatt atgaatacgg ctgtcagcgg
    73441 caataacagt caagctctac gagaacgctt tgccaaagat gtacttagct atacagcaga
    73501 taaagtattt attctcattg ggacgaatga tctagcagag cataaacaac tttctaaaga
    73561 aacgtaccaa aaaatctgca gtggatgata gttcagctga aaaaagacta tagttctgat
    73621 catgctttct ttatcacgcc accagcagtc gatgagacaa aacaaaaata tcggaataac
    73681 aagttgatca aagaatatat ctctgttctc acagaagtag caaaacgcca tgattgtgaa
    73741 gtatcgattt ttatacggaa tttcttgaac aaatggattc gaagaatttt tataatgggc
    73801 taatggatga cggacttcat ttcggcagtg aagggtagga tcttttgtca gacaggatga
    73861 ttcgttatat ttaaagaata ttttgtttaa gcaatgagta ctttttaatc aacttgaaaa
    73921 aacggaggaa tataaaacat gttagaaaaa tcttcaaaag catgcgatac gaataaagaa
    73981 aaagttactg ataagtcaca taatgaacgc attccttggg accagtactt tatggctcag
    74041 gcagtactgt tatctttacg tagtacgtgt acacgtttag aagtaggagc gacactcgtt
    74101 cgagaaaaaa ggatcattgc tggcggctat aatggagcag tcagtggaga tgtccactgc
    74161 atagatgaag ggtgttacgt cgtggatgga cactgcttgc gaacgatcca tgcagagatg
    74221 aatgcactcc tacagtgtgc aaaacttggt atcccgacgg aaggttcaga aatctatgtc
    74281 acacattttc catgtttggc ctgtacaaag gctttgttgc aagctggtgt gaaagggatc
    74341 aattatctcc atgactatcg aaatgatcct tatgcccaag cgttgatcga acaattaggt
    74401 gtatctgtac accaagttgc tttagactca cagcattttt caaaacttca aaatgaatta
    74461 acaggaaaac atgtctagcg ttttagaaag gcatgttcga aatcggttga tttttccggc
    74521 aattacgtcg atgattatct gtttgttgta ttacactgac aattggggtt atcgtttgct
    74581 tcttgccgga atttttttat ggttttcgct aaaagaagga cgttttatcg cgtacttgtg
    74641 tattttgtgt agcataacgg tatccatttc ttataggcta caagagtatt catttttaga
    74701 ggaaaaaaca acatctgcgc aaattactgt atttacagat acgattcgtg caaatggtaa
    74761 ttttttgaca atggaaggaa ggttatcaga gaataaacaa aagctccagc tttcttatca
    74821 agtaaacaca gaagcagaat tgaaaacatg gcttaacttc cgaggaaagg gaatagaatt
    74881 gactgctgaa ggcgagttca tctctccagc aaaaaagcgg aatctttata attttgacca
    74941 agaagcttac ttcaggaacc atagaatttc tggacggttc caaattcaaa agattgaaca
    75001 gatcaccatg caaaatcact ggcgaaactg gcttcagaga aaaagaggat cttttatctc
    75061 ctttgtccaa tctgaatttc cgcaaaaaac aagtgtatat attaattctt tattatgggg
    75121 ctataaagat gcaggattca gagaaccaga agaaattttt agggagagtg gtttgctgca
    75181 tctgttcagt ctatcgggtc tacacatcca attttattta gggtggctgt attatttatt
    75241 tcgacgtatc ggatggccac tacacgtcag tattattccg ttgagtctac taactatctg
    75301 ttatgtttct attgcaggaa gttctatttc tgttttgcgt gctagtttgc tgtttttatt
    75361 gcgattgcta gtcaaattgc tgaataagca ttattccacc atggacttat ttgcagttgt
    75421 cttttggctt cttttactat ttgatcctcg atttcttttt catgcgggtg gacagcttag
    75481 ttttttcatg agttttttgt ttatcctgat ttcttttgat ggagattggt tgaaaaaaac
    75541 ggcctctcac gtcctgttta ctatgatcac gatgccgttg attgtatggc atttttatga
    75601 gtggcctatt ttaggtagtc tttttacatt actgattgca ccggtattca agtttctttt
    75661 tcttcctggt gtgttatttc tcacactctt caatcgctgg ctgccggaaa ctttactttt
    75721 gctgatagaa gagggattaa tcttatttga gcgaataatc gatttttctt cagggtcgtc
    75781 tctcattata ggacgtttgc cccttgtgct atctgggatg tatattttcg gattactgat
    75841 ttgtatggat aggttgaaag aacaacctgt gaaacacttg ttttatgttg gtcttttttc
    75901 ttttattttt ttgactttgc catttttacg cttttcttca acgatcgctt ttatcgatgt
    75961 cggtcaagga gacagtatct ttttgcaaag tccttttcga aaagaaacga ttttgatcga
    76021 tactggaggt cagttacact ttgaaagaga aaagtgggcg caaggtatga tacgtcctcc
    76081 tgctgaaaga aatatcgttc catatctaaa aggtcagggg ataagtgtaa tcgataaact
    76141 catcttgaca cacgacgata cggatcatgt gggagaatta cccacgttat ctcagcattt
    76201 tactatcaaa aaaatttata tcggttgggg cgctggacga aatgagccat tacaaaggca
    76261 tttatctgaa gctcaaaaaa atggtactga gatcattgaa atcaaacatg gggacaggat
    76321 cagcggttat tatgatcttt atgtattgac tccttttgaa aagggagagg gaagaaatga
    76381 agattcgatt ggtctatgga tggaatataa caataggcgc tttttatttt taggagattt
    76441 gaatcaagca atggaaaaac aattgctttt gatttatcct aatctgaaag ccgatgtagt
    76501 caaattaggt catcacggta gccgtacgtc aagtaacaca gattttcttt cccatattga
    76561 agcaaaacat gggattatat cctgcggcgt aaataatcgt tatggtcacc cgcacagcga
    76621 agtattggag acactagaag aaaatcagat aaaaacatgg cgaacggatc aacaaggaat
    76681 gatcacctat cgctggcatc cagtttttca tcctcatggg ctagtggaaa caatgataga
    76741 ctagtaattc tcatctgctc atggtaggat aatttttgaa agtggtgagt aatttgagta
    76801 tacaagcaga gttacaaaaa atacgtaaag aaaaacttgc atcttgttac ttgattttag
    76861 gaacagaaaa gtttctccaa gatcaagttc ggacagaaat tgtcaaaaaa atccaattag
    76921 agggcgaaga tgacttaaat tttttaagtt ttgacatgga aaatgcaagt ttagatgagg
    76981 tagtagctga agcagagacg ttgccgtttt tcggagatca acggttagta ttcgtagaga
    77041 atccttattt tttaacaggt gaaaaagtaa acaatggaat cgaacaaaat actgatttat
    77101 tgacggctta tttgaaagaa ccattagaat cgactgtatt agtattcttt gcgccatatg
    77161 aaaagttgga tgaacggaaa aaagtaacga aacaattgaa aaaaacagca attatcatca
    77221 atgtccaaca gttgaatgaa aaagaagtac gtcaatatct gatgaatacc ttagagaatt
    77281 cctcaatcaa aatggaacga agtgcaattg atttattctt gaggttgacc gacttagatt
    77341 tatccaaaat gatgagagag ttacagaaat tggtccttta tggacaaaat caacagcaaa
    77401 tcacggtaag agaagtagaa gaattagttc ctaaaacgtt agaacataat atttttgaca
    77461 tgacccaata tattttaaaa gggaagacgg aacaagcttt gcgtttatac gaagatttag
    77521 ttatgcaagg ggaagaaaca atcaaaatta atgcgatttt gctttctcaa ttacgtttat
    77581 ttttacagac aaaatttctt gtaaagattg gttatcagca ggcaaatatt gctgaaacat
    77641 tgaaaatcca tccatatcgt gtaaaacttg ccatgcagga agtaaaaaaa ttcgatgaac
    77701 atttgctggt acagttgttt gatcaattag ttgagatgga ttatcaaatc aaaacgggac
    77761 aaatcgaaaa agaattaagt tttcaactat tcgttttgcg cacagggcag ttgttaagaa
    77821 atcgataaga agaaagacga gggcaaagca tgcgggaagg tcgtttgttt ttgtcctttt
    77881 tttgacaaat aaaaaaatcg gccgaagccg attcttttat ttagcgattt ttttgcttaa
    77941 acgagatttg tcgcggttag ctttgttttt gtggattaga ccttttgatt cagccatatc
    78001 aatagcttta acagcttctt tatataacgc atctacgttg tcagcacctg aagctacagc
    78061 atcttcaaat tttttgattg cagtacgcat agcgcttgtt tgagatgaat ttttcacatt
    78121 agcgttttcg cttgtacgaa cacgtttgat tgcagattca atgtttggca ttagtttcac
    78181 ctccgataac ttaagttctt acgcgtgtta ccgcataatt caacattcat tattatacct
    78241 aaaggcagcg tgctttgcaa tatgaacatg acaagttttt ttccttaaca gtcttttatt
    78301 aggatttttt ttcagtaaaa acggatagta caagctgaaa aaaatcaaaa agacgctatt
    78361 gtgggaggca atagcgtcaa accaaactag atttttattt atctatttaa acaaagtttg
    78421 aatgggaatg tttggtacta agttattata taacaaagag aaccgtttgc aaagtttttt
    78481 ttgaaaaggg atacgattcc ctgttgttct ttgtttttct attaagaaac tgcagttatt
    78541 ttataaccag tatcctttga tcagtaaagg ctattctggt gtaccgatta ttttaatgta
    78601 ttcatcgata ttttttatca acattttttt tatgaccaac tggttccgac ggttccattt
    78661 gccacttact tccagcacat atttatttag gggcaaacgt aagatatatt ttgccaaata
    78721 gcgatcttta agcaggccat tgatcacacg gtcctcaagc tgcaagttga tcccagtcag
    78781 catcattggc tgttcttgta aaacgtacgt atcaattaaa agaccttcaa aagtttccac
    78841 aagcgtcttc ctttcttttc aaagtaacct gaaaacaggc actaattctt ccattttgat
    78901 attcgttgaa gtggatgcga tcattctttc ttttgtccac tgtaatctag tataacaaaa
    78961 aagagcagaa gagccaaaaa gaaaaaagag catcgaattc tataggaagt ctcattggca
    79021 cggcctaaat tattacaaaa cattgattaa aaaaagcccc tgcatcgcag gagcatcccc
    79081 tatatcgttt taataggtca atcaaaaagc agtatcaaac acattttgta tcaaatcagg
    79141 aaataataga taagctaagc ccagaatcaa aagtaccgct agaagaaacc agccgattgt
    79201 tcctatgaaa tactttaatt tttcgaacat tgtatcacct cgttgtcagt atatcacgga
    79261 tcagtaaatt gggggtgtta agaatctcct aaaatagaca aaatttgttc tattttccta
    79321 aagcaaatac ttgtaagata cagtaagtga taccagaagc aatgaccggt ccggcggcga
    79381 ttcctcttaa gaagacaaca cctaaaatcg tgccgaaaac aagtgcaaca gtgatttgtg
    79441 gatcagaagc aatcaatcct acacccttgg aagataagac agccactagc ccaccgcaaa
    79501 aaacagctac ccagccaata ggggatttaa aggcttctaa tagatcacgt agaccgattt
    79561 ctcctgttgc gatagggatt agaatagccg ctgtgatgat cgtcacccca atattgattc
    79621 ctttgccttg aagccaaggg aaaaaggaac gggaggctgg tatgagcttc ataatcaaaa
    79681 caaaagctgc cgctatcatc agactttgat ttttagctaa aattgctaca agtaaaataa
    79741 ttcctaaaaa aagccaactt tccatgaaat atattcctca ctttcttcga aacaatcata
    79801 acatgttttt agaaagttac aaaagtagga attcatcctt caagacctat tcataagctg
    79861 tttttttcat aaaataaaca aaatcataaa aaaacacttt gcttatttga cagaagacag
    79921 ccgcgctttg tatagtggac aagacttaca tttttttgaa gaatcagaaa gatcaggtaa
    79981 aggtcttagt aaacgcaagc cattcagaat aaccaatatt gtgcttcctt cgtgtcctac
    80041 aactccaaga ggtagattta aaaattgcag gaagttagaa gcaatcaaca agagaataac
    80101 aagaatcgaa aaaataatat tttgtttgat gatccgccgc atttttaaag atagccgatg
    80161 actgtagaca aatttattta agtcgctttt cattaagacg atgtctgcga tatccatagc
    80221 ggcatctgtc ccttgcccca tagcgacacc taaagaagcg gctgctaaag caggcgcatc
    80281 attgactcca tcaccaacca tcgcatttac ttggtaaagt tcattctctt ttttgactag
    80341 ttgtgttttt tcttccggag tacagcttgc atagtaatgt tcaatcccta cttgttcagc
    80401 aatggccttt gctgtgccag aatggtcgcc ggttatcata cttgtatgga tgttttggga
    80461 tttaaagtag gaaattactt gttgggtatt tgcttttgga atatctagca gacctaaaac
    80521 agcaattaac tgctgatctt tagaaagata aatcacggtt ttgccttgat tttccaaacg
    80581 ttcgatcatt tccgcaatct ctttgctgat tatcatcgtc tcaggatcat atgcgtgctt
    80641 gccaacttta cattgcgcac ctaaataggt agtttgtaat ccaaaaccag taatttcttc
    80701 tattggtaag tattggattt cttcaggtat tgtgcaatcg aaatattgca aaatcgcatg
    80761 agcaagtgga tgcgtcgttt tttgttccat agcaacaaga agttgttgtg ccaaatcctt
    80821 ttcatcaaag aaaaagctgt cagttacaac tggttttcct ttagtgagcg ttcctgtttt
    80881 atcaaaggca atcgctttta attcagaaag cttttctaaa tggataccgc ctttgattaa
    80941 aatcccattt tttgcagaat ttgaaagagc agctagagaa gctggtgtag cagatgcgac
    81001 aagcgcacaa ggagaagcca caactaataa taccattcct cgataaaagc tttcatccca
    81061 actccaacct aggaaaaagt gggggagaag gatcattaga ggaacagcaa gcaaaatcaa
    81121 ttttacataa ttattttcga ttcgttcgat aaatgaagct gtttgagttg gcgtattctg
    81181 cgcttcttct actaattgga taatcttagc aaagacagtt tcggaactcg tttttgttac
    81241 tcgaatcgtg atgggatttc cttgattcag cgttcctgca tacatctcat cgcctaaata
    81301 tttttctaca ggcacagatt caccgcttaa tgcagactca tcgatcgttg cctgtgtgct
    81361 ttctaaaaag ccatctatcg gaacagctgc tcctttagcg acaaatacca agttatccac
    81421 ctttagttga gaaacatcta cttcttgcat ttgtccattt tcttttaata attgggcttt
    81481 ttgtggttga agattcatca aagcagtaat ttctttttga cttttattag ttgtgtattc
    81541 ctctaatgca ccgcttaaac aaaagatgaa tgtcaacatt gctccttcaa agtaattacc
    81601 gatcaaacag gcaccaatag cagctaaagc catcaataaa tccacattca gtgtccgatc
    81661 attccacaat tctttcaagc cttccgatgt ttgtttatat ccaccgatga taatggcact
    81721 gatataaaaa agtggggtga tggtaagaga gaatcggtcg aagataaaac cgaagatcat
    81781 aaataaaagg cagccgattg ttgctgcaaa agcttttttc tgattagtca tcgttattag
    81841 acctccttgt caaaacaagg atagcataat aaaaatgtaa ttgataataa taacagctaa
    81901 atgagaacga taatggttat catttggtga ttaagcttga tgttgttttt attatttata
    81961 atgattatta ttaaagaaaa taaaaaacca gaacatttct caaataagag aagtgttctg
    82021 gttttttcta cattgcaata aaccaataac caatcaaaat gataccaagg ataatacgat
    82081 accagccaaa agcggtaaag tcgtttttct tcaaatagct tagtaagaat ttgatagcta
    82141 agacggaaac cacaaaagat acgaatgttc ctacaagaag aacaacagtt tcttccattt
    82201 tgaatgaatt cccttcagct aaatatttga cgatcttcaa gccgcttgcg ccaaacatga
    82261 caggaatccc taagaaaaat gaaaactctg tagcgacaaa acgcgaagca ccaatcaaga
    82321 tagatcctag gatcgttgcg ccagaacggg atgttccggg aacaagtgcc aagacttgga
    82381 ataacccaac aagaagagct gcttggtacg taaagtcatt cagagaagtc caacgaggtg
    82441 ttttgttttt gttgcgtttt tcgacaataa taaaggcaat accgtaaacg atcaaaacaa
    82501 tggcaactgg taagaacttg tggaaatttg cttctaacca atcatccaaa ggcaaaccaa
    82561 tgatgatggc tggtatgcaa gcaacgatca ctttaaacca aagtgtccac gtatcttttt
    82621 tttctaccgt tgatttactt ggagaaaatg ggtttaactt gtgaaaataa agaacgacga
    82681 cagccaagat cgcccctaat tggataacga cattgaacat cgacataaat tgtgaactgg
    82741 catccagttt gatgaattca tctgctagga tcaaatgacc agtactactg atgggcaccc
    82801 attccgttat tccttcaata ataccgagaa tcactgcttt gataatattt gcaaaaaaca
    82861 taaattcctt ccttcccaat taagatacat cttatagtat accgttattt tttgagaaac
    82921 ccaatctgtt ttttaatatt aagaaaaact tgagaaataa agcagataag tacacgaaga
    82981 gaaaaaagaa gacaaagccc tcgttcagac tttgcctttt atgattctct ggtaaaaatt
    83041 aaaatatccc cattatagaa acgagtcgcg actatgtgtt ttcccgttat cgatataaga
    83101 cgtatcattg atgccgtgga tagaaaatcg accgtttgca taagtgacct ttgttacgct
    83161 gccatttaaa agcccagcac gaacaggctg tttaggatcg atcagattaa gcaaaaaggc
    83221 gatcgtcaaa ccatgagata cgactaaaac atttccacca ccattttttt cgcagtgatg
    83281 ggcgatatct tcaaatcctg tccatacacg gtctcgcaat gtttcataag tttccgccca
    83341 cccagcggta tctaagttgt atacagcatt agcaatttgt tcaaaagaaa cttctgtggt
    83401 aaacatttca tcatatgttt taaaattcaa tacacgaggg atcacacccc acatttccat
    83461 gtcatatcca ccttcaagag acccgaaaca ccattctcta atcctttttt caataaaata
    83521 aggaatttct tttcccttag aatgttctcc tagaataata cgggcagttt cgattgcacg
    83581 accactgtca ctagaataag caaatgaaaa gtcgacattt tttaacccgc gacctaaata
    83641 atgaatgcct tcttgtcctt gctttgtgag gggtgtatca caccagcctt gtactcgttc
    83701 aattgtattg aacatcgttt ttccgtgtcg tacgatataa agtgtagttt ctgtcatttt
    83761 attctcctcc taagtcgttt taacgattga aaaaaggatt tgtccttttt tcctgttcaa
    83821 tcgtcgttgc atcgccatgt cctggataga ctgcaaattc atcaggtaat acaaacagct
    83881 gggtccgaat actatgaagt aattgttcta aattccctgt atacagatcg gttctaccga
    83941 tacttcctct aaacagggcg tcgccagaaa cgacaaaatc atcaaaaata aagctgacgc
    84001 ttcctataga atgccctggg gtagggacga cgcgaaaaga catgtttcct aaagtataag
    84061 ttttcatttc aaattcatat tctgcaggcg agacaataac atcagcaata tcatcatgtc
    84121 taccgagtcc agaaagattc aaaatcggat cacctagcca tttttgctct agtgggctga
    84181 cataaactgg gatttgataa tacgcgcgta aaggttccac tgcaccgata tgatcgtagt
    84241 gagtatgggt aagcaaaatc gctaccggct gttggtttgt ttttttgatt tcttgtttga
    84301 ttcgttccgc atcttctcca gggtcaatga tcaaaagtgc ttcttcgttg tagaccaaat
    84361 aacagttttc ttcaatcatt cctgttttta atctttcgat atgcatgtgc attcctcctt
    84421 gatagccaaa acttctttca tccattagta taacgcattc attgctagga aacaaaaaca
    84481 ggtacttatt tctctttact tatctgaatt atcagactat atgaaaagta tttggatatt
    84541 ctaaagagaa ggaaagtaag ttttcatagc attttaatat gtccttgtta cattagattc
    84601 atccaaaaat ccaaattcta tatcctatat ccctgcctaa tcagcagggt gtttttttat
    84661 gtttggaaag agagctcttt ttgctaaact aggttttttc aggtaatctt agagagaaag
    84721 aggtggtgga aatgatcaaa cagtcaaaaa tatctgtgcg gcacttagta gaatttattt
    84781 tgcgacgtgg aagtatcgac aatcgtaaaa aaagtaacca tactgcatta gaaggagcga
    84841 gaatccaccg caagctccaa aaagatgcgg gagaaaacta tgaaaaagaa gtctttttaa
    84901 aaacaacagt tcaattagat gaatattacc tgatcgttga aggccgtgca gacggtattt
    84961 tcaaaagaga cggtatttat tatatagatg aaatcaaaac ctccgaacca cgatttgaag
    85021 atttagaacc agaacaagtg gaacttttct ttcatcaagc aagggtgtat gcctatatct
    85081 atagtcaaga acatgattta caagaaataa atctgcaatt gacttacttc cagacggcag
    85141 aagaagtgat cacgagaaag gttgaacatc agacaagaga gcagttagaa acttttttta
    85201 aaaagctgac tgaagattat aaagaatggc ttgtttttca agaaaactgg cgcactgtac
    85261 gcaatgcctc tttgatggct ttgaaattcc cccatgatga gtataggaaa ggtcagcgtg
    85321 aactagcggt agctgcttat aaaacgatcc gtacgaaaca gaaattgttt gtagaagcac
    85381 ctaccggaac aggaaaaaca atctctacgt tattccccgc tttgaaagct gtaggggaag
    85441 aagagggaga aaagatcttt tatctaacgg ctaaaacaat aactagacaa gtagctgaag
    85501 atgctatgac agcattaaaa gatacaggag ctgaagtaaa aagtgtcact ttgacggcga
    85561 aagacaagat ctgcttttta gatgagacga cctgtaatcc agatcagtgc ccatatgcca
    85621 atggttatta caatcggatt aatgaaggtt tatgggatct tttgaatcat gagaatcaga
    85681 tcactcgtga agtcatcgaa acttatgcaa gaaagcatac actttgtcct ttcgaactat
    85741 cattagatgt cagtatctgg tgtgacgtaa tcattgggga ttacaattat ttatttgatc
    85801 caactgttta tttaagacgt ttttttgaag atgaaaaaaa cgaggactat ctgtttttga
    85861 ttgacgaagc acataatttg gtgaatcgtt cccgagagat gtattcggca gaactatctt
    85921 atgaaaaaac gaaacgaagt aaggaagcta taccaaagga attcaaaaaa cttcatcgcc
    85981 gtttcaacaa actattaaaa gaatttgatt cgatccgaga aatagcaaaa gaagatcatt
    86041 gggattatca tcatcaaaaa gcaccggctg aatctttagt aaaggcaggt tatcaattaa
    86101 gcgaaaaaat taaagaatgg ctggcagaat ttcctgagca cccatcacaa gaacaattgc
    86161 ttccttatta ttttgatttg cttcattttt tgaaggtcag tgaattttat gatgatcact
    86221 acgaaacaac agtcgaaaaa acgtatcgcg atctgatagt aaaagagttt tgtattgatc
    86281 caagcttgtt tcttgagcag tcgttggata aaggaaaaag cagtattctt ttttctgcta
    86341 gtttttcacc actctcttat tatcaggaaa cactgggggg acaaaaaagt ctggcttaca
    86401 aactgccgag tcctttccca gaaggaaacc agcaagtttt catcgcaaat tacctagaga
    86461 cgacttatcg aaaaagggaa cacagtctgg caaaattagt agagacgatt catgcttttg
    86521 ctgatggaaa ggtcgggaat tatttcgttt tctttccttc ttatcaatat ttagatcttg
    86581 cggtggaagc attcagacag caatatcccg acagcaatgt gctaatccaa gaaacggaca
    86641 tgaatgagga agaacgcgaa cgatttttaa caaaatttca aacaaatccc gctgaaacat
    86701 tgattggatt ttgcgtactt ggcgggattt tttcagaagg aatcgatttg agaggggacc
    86761 gattgatcgg cagtatgatc gtaggtgtag gtttgccgca gatgaatcat gagcaggaac
    86821 tgatcaaatc atattatgat gagaaggaac accttggatt tgcttacgct tatcagcttc
    86881 ctggaatgaa caaagtcttg caagctggtg gtcgagtgat tcgtggcatg gcggatcaag
    86941 gaatcgttgt attggcagat caacgatttt ctagtttttc ttatcgacgt ttattcccac
    87001 aacattggca aacagcaaga gaagtacgaa gtgttgggga attggaagaa ggaatacagc
    87061 gtttttggtt atcaaaagaa gcagtaaatc aaaaaaacga ggaatgacag acgtcagcct
    87121 cgttttttga ttaacgatag ttagtgaatt gcaactcgat tggtaagtca agtttacgta
    87181 ataaagcaat cacagcttgt agatcatcac ggttcttacc tgtcactcta atctgatctt
    87241 cttggatttg tgcttttact ttcagttttt catttttgat agctgtagtg attttcttcg
    87301 cgttttcacg atcgattccg ttgaccaaat cggcagtttg tcgagctttg ccacctagag
    87361 cgtgttctga ttcggaaaaa tggatatttt tgattggcac atttcttttg atcagcttac
    87421 caaataatac atctttgatc tgttcgattt tgaaatcatc atcgctcaat acaactagat
    87481 tgctgttctc taatttgatt tctactgtag aatccttgaa gtcaaagcga ttggacaatt
    87541 cttttgttgt ttgctgaatc gcatttttta cttcttctgt attgacttct gatacaatgt
    87601 cgaagctagc atcttttgcc ataggaaccc tcctgatata catataaata tttaccatac
    87661 atctatctta tcataggatt agggcagatt cttacctgtt tgctaaaatt cttctcattt
    87721 gagttcgaag tattttattt taatagataa actgttattc ttacatttgg aataccaaag
    87781 taggggagag gtgcttgtga gtaaaattat gactgcgtat cataaaaatg aaagattaaa
    87841 gaaaatagtg gttattttga taaccggatt gaccgctgcg gtcgctttaa attatttttt
    87901 gattccagcc aatgtctttt ctgcagggat gaatgggatc gctcaaatca tttcctcgct
    87961 tttatatgaa tggtttcata tcaagataga cacaggaaca tttatttttc ttttgaatat
    88021 ccctgtgttt gtattagggt ttctcaaagt aggaaaacga gcaacgatcc ttagttttgt
    88081 gaatgttgta tgtgtatcgg tgatgacgat gctgcttcca gtcggacaga tcactgataa
    88141 tattttgatg aatgccctag taggcggtgt tttattaggt cttggcgtag gattatcttt
    88201 aaaaatgggc tttacaactg gtgggatgga tatcatctct cttgtacttt cacaaacaac
    88261 tggaaaaaca gtcggaaact acatgctgat tctaaatggt atcattgtag tcatcgcagg
    88321 cttcttcttt aactgggaaa gtgccttgta tacgattatt tccatttatg caatgactca
    88381 agtaattgat gccatccaca cgagtcatca aaaagtaaca gcaatgatcg ttaccgtcaa
    88441 gccagaagag gtaacagaag aactagcaaa acggatggta cgtggaatga cattactgcc
    88501 gtctgtcggt ggctattcaa aaaaacaagg gaacatgatc atgatggtca tcacccgcta
    88561 cgaaatgtat gatcttgatc agatcgttca tgaaatcgac gacgatgcat ttatcaatat
    88621 tttaccaact cattcagtat ttggccggtt tgcaaatgaa aacgaacaaa aaatgtatcg
    88681 aacaactggc gtattgccag aaataaaaaa tcgttcacaa aaaaaatgag gaatcaggtt
    88741 tattttagaa taaatttaat ctattacatc taataagaac atttttataa atttaaacga
    88801 gaatcttact tctaaattct catttacaag cagctaaaaa aagagtatga taaataaaaa
    88861 tcgtgttaag gagtgtcgta cgatgagcga agcaaaccaa gaacggtctg gacaagaaat
    88921 agaactaatc aaagaacgta ttcttgctgc cttggaaatg gtgattgatc ctgaattagg
    88981 aatcgacatc gtcaatttag gcttgattta tgatatagaa ttcaatcctg aaaatggaga
    89041 aacagtcatt aagatgactc taacaactat gggctgtcca ttagcagata ttttaacaga
    89101 atctatccat gacgcattaa aagaagtgcc agaagtaacg aaagcagaag tcaaactagt
    89161 ctggtatcca gcatggacga cagataagat gagccgttat gcacggattg cgttggggat
    89221 acgatagaaa tcttggctga ctagctatga gaagcaaata ggaaaaacaa aaaagccttt
    89281 tatcctttct agaagaaaag agataaaagg tttctttgtt tataaaattt tgtctgctat
    89341 attataagtt aattaa
//
LOCUS       DAZABD010000004        80776 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000004 DAZABD010000000
VERSION     DAZABD010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 80776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 80776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..80776
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.004"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..229)
                     /locus_tag="ACHALG_000263"
     CDS             complement(<1..229)
                     /locus_tag="ACHALG_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFN2585707.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSS
                     SYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTI"
     gene            502..2973
                     /locus_tag="ACHALG_000264"
     CDS             502..2973
                     /locus_tag="ACHALG_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFN2585708.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(3076..4812)
                     /locus_tag="ACHALG_000265"
     CDS             complement(3076..4812)
                     /locus_tag="ACHALG_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFN2585709.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSVFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(4809..5513)
                     /locus_tag="ACHALG_000266"
     CDS             complement(4809..5513)
                     /locus_tag="ACHALG_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFN2585710.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            5644..6147
                     /locus_tag="ACHALG_000267"
     CDS             5644..6147
                     /locus_tag="ACHALG_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HFN2585711.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            6107..6445
                     /locus_tag="ACHALG_000268"
     CDS             6107..6445
                     /locus_tag="ACHALG_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HFN2585712.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6466..7884
                     /gene="ffh"
                     /locus_tag="ACHALG_000269"
     CDS             6466..7884
                     /gene="ffh"
                     /locus_tag="ACHALG_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFN2585713.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            7996..8574
                     /locus_tag="ACHALG_000270"
     CDS             7996..8574
                     /locus_tag="ACHALG_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFN2585714.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8578..9099
                     /locus_tag="ACHALG_000271"
     CDS             8578..9099
                     /locus_tag="ACHALG_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HFN2585715.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(9178..9732)
                     /locus_tag="ACHALG_000272"
     CDS             complement(9178..9732)
                     /locus_tag="ACHALG_000272"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFN2585716.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            9985..10260
                     /gene="rpsP"
                     /locus_tag="ACHALG_000273"
     CDS             9985..10260
                     /gene="rpsP"
                     /locus_tag="ACHALG_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFN2585717.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            10272..10523
                     /locus_tag="ACHALG_000274"
     CDS             10272..10523
                     /locus_tag="ACHALG_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HFN2585718.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            10648..11439
                     /locus_tag="ACHALG_000275"
     CDS             10648..11439
                     /locus_tag="ACHALG_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2585719.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            11609..12130
                     /gene="rimM"
                     /locus_tag="ACHALG_000276"
     CDS             11609..12130
                     /gene="rimM"
                     /locus_tag="ACHALG_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFN2585720.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            12120..12881
                     /gene="trmD"
                     /locus_tag="ACHALG_000277"
     CDS             12120..12881
                     /gene="trmD"
                     /locus_tag="ACHALG_000277"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFN2585721.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            13017..13364
                     /gene="rplS"
                     /locus_tag="ACHALG_000278"
     CDS             13017..13364
                     /gene="rplS"
                     /locus_tag="ACHALG_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFN2585722.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            13735..14397
                     /locus_tag="ACHALG_000279"
                     /pseudo
     CDS             13735..14397
                     /locus_tag="ACHALG_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312947.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            14586..14942
                     /locus_tag="ACHALG_000280"
     CDS             14586..14942
                     /locus_tag="ACHALG_000280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2585723.1"
                     /translation="MAESNVFVLYTLADLKTGTFVNYYKIFNEIERYYYLPIEAGILY
                     GLMVDRLMFNEQDERTLLDKEGNSYILMTVKEMQFYIKVRSEQTIRKIIEQMEKEELF
                     DSLNGTNTKVNFVNTW"
     gene            15180..15611
                     /gene="traE"
                     /locus_tag="ACHALG_000281"
     CDS             15180..15611
                     /gene="traE"
                     /locus_tag="ACHALG_000281"
                     /inference="COORDINATES: protein motif:HMM:NF041439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFN2585724.1"
                     /translation="MDRNQAFPERSGPQVKDQGIFRMTDIQRLGADCQCFPGFETVSL
                     SGGKTRFVRRLRTFRVEDRDVVEFEEYHEGKERKIYEKQTLPLKAIYLEIKRVTDVSG
                     KTIARRLKPEQTLRWLFSTGRQSKGQKLNTKTLDVLFFDVP"
     gene            16188..16421
                     /locus_tag="ACHALG_000282"
     CDS             16188..16421
                     /locus_tag="ACHALG_000282"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2585725.1"
                     /translation="MKQEKIQARRRNDEQKGISPKKQLLKEPKAMGALGQKKDQKIKV
                     AFLDGKVMVGTLVQLMKYELVLEVEGKERPIAV"
     gene            16470..16631
                     /locus_tag="ACHALG_000283"
     CDS             16470..16631
                     /locus_tag="ACHALG_000283"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2585726.1"
                     /translation="MARSERERTPKYCFTGETKSYQHRTLYRIQAFGTVKAGEISDSL
                     HKKHFQRKL"
     gene            complement(17237..18427)
                     /locus_tag="ACHALG_000284"
     CDS             complement(17237..18427)
                     /locus_tag="ACHALG_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004913986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFN2585727.1"
                     /translation="MTLFVGIDVTKNKHDFSVIDSFGTIHLNHKQVKNDREGFTELQI
                     TLESLRKSTNEASIQIALEDTGHYCFNLIRFLRSCGYPTFSYNPIIIREFAKVDSLRK
                     TKTDKSDALTIAKKLLNDLDPSIHEVDPFYQELKFLTRHRSRTTEKRAQLKTQFVRLL
                     DIMFPELAPIVGKANIHSQYIYNMFKAYPSPKKISRCRNKNLFKVLTNHRGRQLTSET
                     IDRIKEASKQTIGFSTTSLELELLQIIETIELYTTQIATIDTETDLLMAKINSPITSI
                     TGVKNRLGSVILSEIRNIHNFKSSGQLLAFACLEPSVFQSGQYNREGHMVKRGSTSLR
                     WALIQAAKLAARFSPTFKAYLDKKRAEGKHYSVAITHVAKKLVRVIFHLLKHQIFFEE
                     VKLN"
     gene            18696..19037
                     /locus_tag="ACHALG_000285"
     CDS             18696..19037
                     /locus_tag="ACHALG_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2585728.1"
                     /translation="MKFKNEQAEKEWQEFRNNNQDPYGSGLVRFAERWAELMEKELEA
                     GKPLIDIAGITENQADIEGITGFVYGVAVNVLSHAWKYGEDLRDWHNRKYSYDGSGVV
                     NPVIMSAGKKE"
     gene            19095..19481
                     /locus_tag="ACHALG_000286"
     CDS             19095..19481
                     /locus_tag="ACHALG_000286"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2585729.1"
                     /translation="MKKIISVVLVSMGLFVFTGCTTDADIASNNLSEDADNFKVLRKI
                     TFINTVTDEVLYTVEGNFSIAADTEDNQLEITAKTGEDQFQKYFLGLSPTTVYIVEQL
                     EWQETNQYQFKITIKPSAVLPDVDIR"
     gene            19928..20149
                     /locus_tag="ACHALG_000287"
     CDS             19928..20149
                     /locus_tag="ACHALG_000287"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2585730.1"
                     /translation="MNKEVNGCVVFEKQGNDFSVKAGNLSAADALTLTLFSIVSLAKA
                     SGISLECIHKTLDQIDQHLVLEECGQKNE"
     gene            20142..20495
                     /locus_tag="ACHALG_000288"
     CDS             20142..20495
                     /locus_tag="ACHALG_000288"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2585731.1"
                     /translation="MSEGNATVKKLARKELFELTDEERHPNWADDPQAIKRRDQLLVI
                     LGMPIDPVRKEGETKEAFQKRSNQYFFDLRPGLEEKVVSRLLAGEKMKRLSEEYQLPR
                     SKLTYLREKYHLLEK"
     gene            complement(20509..20940)
                     /locus_tag="ACHALG_000289"
     CDS             complement(20509..20940)
                     /locus_tag="ACHALG_000289"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2585732.1"
                     /translation="MTKLSKYLFGGLLILVGLLAFLAFDHLKEKPKETLTVTESTFST
                     DNEKKTELFGFTQLQEKEGDNLIDLGIWQIETIIFKGKTKAILTTANGKELSFPIEIT
                     NQDQNEITLPTVKYASKNLIIAQQIGLAQKDNHYFMYQVAQ"
     gene            complement(21525..21770)
                     /locus_tag="ACHALG_000290"
     CDS             complement(21525..21770)
                     /locus_tag="ACHALG_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016617603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2585733.1"
                     /translation="MDTESIKSFQIWAQENLVTRKGAAKITGQSYAGISQAINRKVIT
                     PFLEFDGDPATSLVRLYLKSDVEAYAKQLKAKKQKQR"
     gene            21919..23073
                     /locus_tag="ACHALG_000291"
     CDS             21919..23073
                     /locus_tag="ACHALG_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFN2585734.1"
                     /translation="MKLNVLRVLAVGIVVGGIALAMNTEKAEAAEWSPRSVEAIKADI
                     AKAKDNQYTIVWGDTLSGISQATNLTVQKLADLNKIANVDLIYAGNTLVFDGNVVTAK
                     NDKGETVAQSVVTPQDKNDANKPVGKQAPAKQEEKVLGTTASSQSQNKQGTPATSGKA
                     DTNKQGTSAAVGEYGQLEGFYSKEYVENGWKYVEAGPFKTGKDFLNWFDATYPGKGGP
                     GYFILSGEQDFTIGDNVNLVAKKAIEYVCDSGNTTTENAETPAQPSEPVTPSQPTDNQ
                     STQPSKPAEKPSTSTQPSTPTQPSTGGEQGGSTTPTTPDTTPINMGNSGKVFDSEEAA
                     SEYGYAEIANEKSPWYGYRFGASQLVNKDYELLGTWTVDFTENKPFDWNE"
     gene            23251..28185
                     /locus_tag="ACHALG_000292"
     CDS             23251..28185
                     /locus_tag="ACHALG_000292"
                     /inference="COORDINATES: protein motif:HMM:NF037291.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HFN2585735.1"
                     /translation="MRTRTKKKWLKAAALLAFSQFCFLTGTFQAQAEETTPSQPAVET
                     AVAAPVEAVEVQPVEEAVPAVETPAEPMPETDAPATNSKAALNQTIGEAQNPGDSPAV
                     TQPVEPEAPKTNSLPESSEESTSNTTSSSEPTESAEIPEALTPEGTPTPEIPVSPESS
                     VEKTTDTPATDIEKPAEQPVDTKDTEEKTEASEVVSDTKETTLSDKEVEEMFVVTQPR
                     AARIRRAAVSTRAANDNIVRENLVGQGYNWAVVDDPGRKGFTFNAIPMFTVNGQAAFC
                     IQPGVDFTANGQVHSTEALDTYLKDANKRSQISLISYFGYVNNPDKSKEQYFTTQLMI
                     GEILGRKVVLTNGLNYEARKAAIQKLVDQFHTRASFNNQTKTVKVGETIKVTDTTGFL
                     SRVKEVKAPKGITAKIQGNQLVITVTKDAPEKAQVRLAQIKLASAPMVYKKPGSQTIG
                     VLNPTEPGDSILNLNVLKQGNLEILKIDADTKEPLANAEIKVTIAGKDQTVKTNDKGI
                     ATIKNLTHGTKGTVVEIKAPSGYVLNKTSKDFTIEANKTVRVTLENKEQKGIARIFKE
                     DAETGNNAQGAATLEDAVYGLYQADGKKIKSVTLKNINGRVQAEIADLKLGDYYWVEE
                     QAPVGYNLNTNKIHFSLVYGDQNQETVTTEVIAKDQVIKGTIEGVKVGNKDFVDANDP
                     DNKPKLEGIKISLTSKTTGKVVKAVLTDKDGYFTFGKNAVVVDTYIVSETKGKEGYKL
                     FEPFEVTISEQGQTFRYVLEDQIIEQQLKIVKVDQETGKVIPLANTEFKIFDTLANEY
                     VIFSIPNDTEVTDIFRTNDKGYLVTNGTFTYGNNRYRIEEINAPRNYVLNKTPLVFSI
                     TDDTEPIKVINFANRQARKNVKLFKYEEWNKKKTPLAGVMFTLYNGEGKALGEYTTDT
                     NGELLVKGLVAGSYYFVEKAPLESFQPNTDKQAFTITFKQNAPDDGETLLVSVRNYLI
                     PPTLETQASNKEDGEKVVDPLETIVIKDSVSYTNLFVGKEYTISGVLMNKATNEPILQ
                     DGKEIRKTLTFIADKANGTKDLEFVVNASVLKGQTIVVFEKLFRDGIEVAAHEDITDE
                     NQSVRVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITEILPGKAYLVKGILMDKAT
                     GEPLLLYGNTVETSVGFIAKEENETVIVPFTFDLTDLNGREIVVFESLFRNDEELANH
                     KDINDKGQTVRITNPAIGTKATDKETGLQVIDPLAVITITDAVAYHDLIVGKTYTVNG
                     ILMDKATEQPVLVGDKEITSTLTFVAETTDGTVNLDFVLDARALRGKEVVVFEDLFRD
                     GVLLASHADITDEGQTVRVTNPGVRTTATHKTTGLKEVNPLTKVTITDAVKYHDLIIG
                     RKYTVNGILIDKSTKKPVLVDDKEVTSTLTFVAESTDGTVNLDFILDARALRGKEVVV
                     FENLFREGVLLASHADITDKDQTIKIVNPTITTKATNKNGGKEIFALPNQTVLEWVKV
                     NGLAIGQLYKLIVQGYLTPDGTPFEGTHAEKIFTADKETTEFLFEFLVDGRNLAGKGL
                     SFAEWLQAKTEDEKETFEEVARHNVDLTNKDQTVQIVEAPKPPQPEQPVKVASVVPTL
                     PKTGFQDNGVWVVIGLIFVLAAGLIGHDLYKVKD"
     gene            28392..28763
                     /locus_tag="ACHALG_000293"
     CDS             28392..28763
                     /locus_tag="ACHALG_000293"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2585736.1"
                     /translation="MFSCSDCYTAEELLEGIRMYQEEHEWAESLTLSWEYEPIDPSVI
                     QAIEEAGYFCEAHFSALHNISYYKCYFVPVPEKQRVCNQQRIKEQEEQRKKERRWDIL
                     ETSLTIFVLLFFIVMGYVLCV"
     gene            29001..29495
                     /locus_tag="ACHALG_000294"
     CDS             29001..29495
                     /locus_tag="ACHALG_000294"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2585737.1"
                     /translation="MSEMFQSDRILHLSLGLDVLLNYNKEGTKIKTVQLSKSRLGELI
                     RNQTVTRTSIMKNQKIGLIIGCNTNIVINVEDLIGFQDKTPFILNIDSDQTEQMYEGK
                     DIFQMSHFEFIVSNEQDLRPSQIKQLYYINRKFQDYLQEQEAAKKAKKRKAAIRRSVI
                     ANQK"
     gene            29514..31382
                     /locus_tag="ACHALG_000295"
     CDS             29514..31382
                     /locus_tag="ACHALG_000295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2585738.1"
                     /translation="MSEGKETESRITTNTKKKIAIAVALGVVLFSGGLGIHLLHSPEK
                     NESKEIEYTTISSEKSLENPSIRNADKATKRSKTKIVEKNQVDRKKESKSKENNSQST
                     LFDSIFSRIDESNEEPDQTKEALTQTQPLLTLPELKEIAKVTEEKEKQKELLKSKQKE
                     NAELVLVGKEGEETEKDEGVEPTPLSTPTPILDPSLEPEPTPILPVESKPKIVVSQSY
                     FELNEGDSFDISNYYSVFDSMDQSPSVWIENNTLSVGTNTMTIHAVNKFGYSDTETIT
                     VQVNGRPKIQAISSNVEVSIHETIDLATYFSATDPEDGNLQEKIEFLSTVNTGIEGEY
                     LVTATVKDSRGVEAEPTTIRFFVKNEAPVIQGESSYTVSIDESFSILNLLSVTDKEDD
                     RDGYPINLTEENILENDLDLTKEGIYTIKIGNVADRDGKKAEDKGITVNVVNEAPVVM
                     ASDQTLKIGDSFDPQVFLNSISASDKEDTKNQLTPTISVDEETLYSIRTDISGVYKIA
                     VTAIDSQGKATTVFATITVVEDDSATTESPETKENELDIPSVEAQSDQIAPEEPLTEK
                     KEHSPLLPTENHIDSVEEAPEESEETSLEEKQGQGDSAGGSELESGDQVNVQNPSV"
     gene            31478..32293
                     /locus_tag="ACHALG_000296"
     CDS             31478..32293
                     /locus_tag="ACHALG_000296"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2585739.1"
                     /translation="MNEKELAIKRLLISILEAIFEPSDVYQGFIVRVIPKELKREGQY
                     KHFERTIQLNNLTRSPLELCLTCLRCLAMHVDTVNSEFFYESEGMYPILQKLLEASLE
                     LGIFSIREIDELEQRGKIDDIVANCGNSAVWTLKYPLLPVAYDVYVSNGYAIKGELRR
                     WSYDYQKEGKVWHKSFSSQEEAIQEKERLWETAAEIKVEVTTKLERTFDFAYYVAVKP
                     ALELNDLFQEHGYAYEAYGVKKHYVKKVPTKKFFQEREFLMLYEVPFTIKLPK"
     gene            32309..32533
                     /locus_tag="ACHALG_000297"
     CDS             32309..32533
                     /locus_tag="ACHALG_000297"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2585740.1"
                     /translation="MATKKERSEIKKKTIKLMSLEGIDYEEWLHEQHVKFVDEQGEKI
                     LDRLMDEKLQQTKKSDVGQPTQIIHGGEEK"
     gene            32530..32859
                     /locus_tag="ACHALG_000298"
     CDS             32530..32859
                     /locus_tag="ACHALG_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008381241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2585741.1"
                     /translation="MTKKRTGYQWLGVAFVLWAMAFLFFTVDLTAFADVQTTLKDIET
                     GAGTQFKAIANIAIGIVVILFGGAQFLGAEIKSWAKKLLIGAIVGAIVIVNFDWIRDT
                     FWGWIGG"
     gene            32863..33405
                     /locus_tag="ACHALG_000299"
     CDS             32863..33405
                     /locus_tag="ACHALG_000299"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2585742.1"
                     /translation="MVEVEILNAQIKGFTLPLVPVEWEVFLEVTEAMNPSEMLSFQLL
                     EVGGASFYQGKFEKQSSLESFEADLKKKLKLLVKKKGITKEEAQQILELTGFIDKKPS
                     LSTKQKDEAAFAKISKSSRKGNISLNQRSKFLDKTFLTYILVGIALFFLIGAGVYFFK
                     DRLFQEKRGQHLPLLRVLKQ"
     gene            33468..33926
                     /locus_tag="ACHALG_000300"
     CDS             33468..33926
                     /locus_tag="ACHALG_000300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2585743.1"
                     /translation="MNFLVEQEAFDQLEAFQEAYPTEIGKFEVAFNQKDWQTVIEADI
                     STLNNARKMMMAIALIQLGQLDEARLISESLGDTRLFMQLAVEYLRQKNVEEAKKIQD
                     QLNDDQLAEMIDTTEIYLQMITHYAKEKDSKNQELWERKLEQIGKEYLPQ"
     gene            33955..34587
                     /locus_tag="ACHALG_000301"
     CDS             33955..34587
                     /locus_tag="ACHALG_000301"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2585744.1"
                     /translation="MLTKIRDYRAQRKVRKWNRSLAQENNTALAMGKGVRYHHKNLNR
                     IISGHAQHPKKKINGYDPTDEVLEHSLVLDSSRNGKFYQTDITQWGSVDPFYSKSSFL
                     LVDGICRYLEKQYDQVISEALLVQELIVLFKEKNQQNVNADVIWKKIIEASDYSSIVL
                     DVIKEPLLEEVFAFLNAQIVTQLAEGAIKKDETFSPRITVLFDSLSMKYH"
     gene            34751..35578
                     /locus_tag="ACHALG_000302"
     CDS             34751..35578
                     /locus_tag="ACHALG_000302"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2585745.1"
                     /translation="MVSILIKIYDSLKKSKVGIALLLILAILIATTVMVFGLSKIIYH
                     IADRPVLLLTEVIYLVYELLRAPYPLEYDPYISLIVSLFIMLIGYVSLVLMTEKKGEK
                     RYFLFKESHIYVNLSEMKQFYIFISLTIGLTYFSLYYYSFPLSFDSERIQIFLWLGIV
                     LGILWILLAVQQYIFIFEFLFCYNSLAAIRNKHFCNRLCNSLENDGEFKLSEAGVNTD
                     FRDCIEKNLIKIVNDDSLSNLKSNSKIYIHFIKPFDQICKDRENILSEGLFLDYYFE"
     gene            35692..38271
                     /locus_tag="ACHALG_000303"
     CDS             35692..38271
                     /locus_tag="ACHALG_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008381255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HFN2585746.1"
                     /translation="MTKKNKQKEISKDHKKNYEKEKKNSLRFLANRKFLTYFSVIAFL
                     VLTTLGNWLFHRCVAFFSQSLFEFTVERSHVSYGQTIFYYPILRYPFFYFLLFIGIVT
                     FIGYFDYKLYTSFRPLEDDLTQGTMHFTPEEDMEKEYEKVPVHIFAEDEDFYDGKPGY
                     PVGRKPQTPEEKEQGIFYYYVDTSDSNSATLAGTRQGKGIFFVDPFIDILTRARLIAN
                     RASFVMTATKGDEPRKWYKTLKRRGYNVRICNTVNQYFSDPIPTLAVFNNYYRHYKRL
                     KKKADKAKQEEDKASRMKYNVLAEQQLANAEKAINQASFLYFKEVNEGKDGGFWTKAC
                     RNLFISTGLALADQEFEQNEKMKVNPYTIYTVVNDMMSKHIKEGSHDFLNQFAKDKKD
                     LQRLLAEYEDKSYLDVFFSEMPRSHPAKRYFDAIKASAPAQVTLGNIVTHFDGDLQQF
                     LMSANAKMTAYDDGFDLESIGFDRERPTAVFVVLSDADSSNNAVGVNYIDQIYQVLLN
                     RCNLEDDSACYRAVHFIYEEGGNLGVAISDLARKWTSGASRKLFQHLVLQDTEQLTSL
                     YDEQTKEIILGNTANLVYIRTGSKTTNEYISGRLGTRSNYSKTRHKDPTSISSTETES
                     SERIDLLASFELERMRAGESVVLRINKHKDLNGNPIYQYPILNSQENDSNMIPFYEYR
                     KLDKVSWEEIPVNNQFVEMELEELDWSLMPQQEKATQPAVIQKEVPPIEMYKKPIPKK
                     EGTQLSLLSLEELIQLEKNSGKEERRKKEEKAKVLERLYSVEERKQSVQTYYSKEQVE
                     EIQKIMARFFVGQKEVLQELKQILENGQVQSLCTFLLSTSEERMINRYIQQFREWKEA
                     AYE"
     gene            38264..40393
                     /locus_tag="ACHALG_000304"
     CDS             38264..40393
                     /locus_tag="ACHALG_000304"
                     /inference="COORDINATES: protein motif:HMM:NF045890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
                     /protein_id="HFN2585747.1"
                     /translation="MSDKEILEKLLEFAPFLSIQSMIKDFGRSILYWITSVLMWIVEK
                     VGGVLTEMVSVLGFYNATDMTGKGGILDTFSAFQKFGIGITLLIIGAVLFLGKTEETR
                     AVPINALLMVVIVMLLPSIMSDGMKIVNSTVDAVSSEQAEFGFDSFKKNVTDVYVLAD
                     NGWTTTEPKNKNYLEDRDFFDINEKITDAEEIDNGEPLLYEVVNSKSGSGKELQKFDS
                     DGGGITDWLMKKLFVKGYYRWSVNWLPLIATLACLAFSLVISLVRCGRLGIELGFNYI
                     WANIVAYFSIRNIKFLKTAVLELFSGFVLILSIFGMYAVFIAYNSWLGIQQLSVLAQI
                     FGIIGGAWFIYDGPSIIQKTLGIDAGLSTAGSFIMGIGALKAVKKAGEMTGKTVNGAA
                     NTAGFVKGLTEGMPSKEEPKNDEKAGLNSEMAKNTNEDLPEENKNGSNNQQPDVQNQE
                     DSNENSEELDQLQGNEPSTREAETEQETKEDQLPQTPENQSVELQEEPEARIEENLEL
                     SENDANLEQTEPYANDYENNMEDDHNNNSVDYFEGESSGSPHVEEQIPLTDQPESNND
                     QSNLSTSDNEVPINESTATSLNSGDVESKKIGNEKDSAVKNNSMTKNIHGNHVENKKP
                     ENDKDEELPNPFVKKVNQMIHTPNYQKNTKNKVGNMVDGYNRNKEFGQEVNEWLNQRR
                     NKREEEHPESVAPYLERKRAKNKNKSG"
     gene            40405..40797
                     /locus_tag="ACHALG_000305"
     CDS             40405..40797
                     /locus_tag="ACHALG_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008381257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5592 family protein"
                     /protein_id="HFN2585748.1"
                     /translation="MHDIQNEIFTETKFWGAILLKDSVAGFLIFNLARNVQDATYGPL
                     QGVSFWFVILMGFFWLCPSVSNAKLRNYQRLFITLTNNRSTYHAIDRMDFEVQERIDM
                     VMGTVEEMEKEDEMLSLEELETERLDNV"
     gene            40877..41020
                     /locus_tag="ACHALG_000306"
     CDS             40877..41020
                     /locus_tag="ACHALG_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HFN2585749.1"
                     /translation="MEVSVHSLERRSLLSVAEALGLMISFGSLIATIIFGILTAVKND
                     KKK"
     gene            41358..42053
                     /locus_tag="ACHALG_000307"
     CDS             41358..42053
                     /locus_tag="ACHALG_000307"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2585750.1"
                     /translation="MSESIKSLVQSKGFWKIQLLNALWLGRYYFRVCCKVIASMILIN
                     FFFEGVALTSDQKHLFLELVFNLFFMMMGLMLILKSIEFITKRTTDIKSFIVVVLRFS
                     IFMMIFVQISGLEVKTYDSILQGFNQNNFDYLIDHYGRYICFACLYLVSYKFYYERLF
                     VSAIDVNSFKGNHLSWNASDWLVCFVSPNYIRLEKKGRIKVQFCLDYSWIRINHSHSL
                     ETIEQFVPEKMRR"
     gene            42130..42273
                     /locus_tag="ACHALG_000308"
     CDS             42130..42273
                     /locus_tag="ACHALG_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HFN2585751.1"
                     /translation="MEVSAHSLERRSLLSVAEALGLMISFGSLIATLIFGILTVAKND
                     KKK"
     gene            42489..45053
                     /locus_tag="ACHALG_000309"
     CDS             42489..45053
                     /locus_tag="ACHALG_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008381259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trse protein"
                     /protein_id="HFN2585752.1"
                     /translation="MIDSEALLKELDKQETIPKVVKDPLLEEKPKKVRRLKQEKTVLS
                     LIPYRRIISEDCFAMEKEVTNFYRIEDASARDGSEQQRQEALHDYLVFLRTVHKNLKL
                     IFTSFPVDMSENIDYARRRFKMRKGNKNVQAEQAITLSQLEYLDNNKPINTVYLQIFA
                     TDKDELKDVEREIMSVSTANFRPVKMSLTQKIKLLVRLYNPLSTIPIGIPYQDTDETG
                     RRPDVQKIVDKKGYDPLFIGQIQPLGGLRPYSPTELQIGNGFIRVLNLLVYRPKNNPN
                     FWGQHIFNMKNAVTTLDMRSIDVTNPLVEKNLNNSLEEYEDQVVTSKSRISKKKAKNE
                     YYKLDQTVEQILEGQEVLKEIHVRYILSAESIEKLNEKEKEVHRQLGKRQFQASCFLD
                     EQERQFKASFVSFAQGRKLIRRKGKEIKGSALAGSYPFHFTNHIDPDAPYSGYPLYGS
                     GIICLSPTHKDHQRKSYSMMIIGSQGFGKTTVSKKLAKQFVEYGNNHFGFYMSDETKR
                     LTEYLGGIHLDGAEARLNPCQIYASDVDAKTKKTKEQQSYLTNLTKMRLIFALNNEIK
                     SDDPVMVEAKKLFPKAYAKYMEDHNLDLSKITQYAPEQYPIYSDILDFAKKEQAREVE
                     EFRKKSLYQLVSQLESFIDNAGNIFNQRSEFDFSHRQLVTFDMQNLIKSDRATYNAQY
                     YNLYTAAFSEAVRVGQREKYLFDRKQKRVDELIYTNITSDEFHNPIRTENLTLLNELD
                     RYNREARKLLIGQTFILHDIADAFPDYTDGKMGEISKAVMNLFKLTTYRFLLQQDSSS
                     EELMKKVFGKQLSNYELAEIFRFEERDILLNIKGSTNIKFRYDLTEREKAIFDGGL"
     gene            45053..45247
                     /locus_tag="ACHALG_000310"
     CDS             45053..45247
                     /locus_tag="ACHALG_000310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2585753.1"
                     /translation="MKNKNEKKSLKKEKRARHWQKTKKLTRRIVIGKPGEKIQTTKTK
                     TLYLIVVIVFFLYLLLQLFV"
     gene            45268..46422
                     /locus_tag="ACHALG_000311"
     CDS             45268..46422
                     /locus_tag="ACHALG_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008381261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFN2585754.1"
                     /translation="MSQIKVFTEKQLNYARKTNMLSYMLERGEPFRQVSSKFVEHREH
                     DSLRANIKTGVLNWYSQNLTSWNNAIEFSMAFFNESFETTVEDLLQFQRTQRVMDRST
                     NRSVTNKNVLENKLEPFTLTHLSTMGNYDTNVLSQKGREYLRSRYLSDETIGYLEKLN
                     LVSTDNKNNLLFKHIDIGIGKTGNIVGCDIQGTYARSLDKRVSLNDKGKLKLDRKYFK
                     GMGENSMSKRGFLFGVNVAYSVPLTLFVTEGPIESASLMELQKESLPINTWFLSLSGL
                     KEDTFWEVKALLQELTKAPKVESILAVNNDEKGQEFVQNVHETYQDEKEYQFAHTLKL
                     LLPELENGDWNEQLELLKTGVLNSRSEKLKEKREAEKLVQSMQLSYMERV"
     gene            46425..47537
                     /locus_tag="ACHALG_000312"
     CDS             46425..47537
                     /locus_tag="ACHALG_000312"
                     /inference="COORDINATES: protein motif:HMM:NF025081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HFN2585755.1"
                     /translation="MRGLKIKLLVVVLLSVFTFSAVEMVIEETQNMLAGILGIEQTSQ
                     TVEISGNKALSAEVLALKSIVEKEAQAAEIPSEVPFLLAIMMVESGGRGSDPMQSSES
                     GGLSPNSISSVEWSIRQGVKHYKTTLELAKRYGLENDKKAIVQAYNFGSSYVSWLGNS
                     KKKHSVDEAETYSRTVVAPSQRTNGYPNAGQKYSYVNEVSKADGRTYLYRHSGNFFYA
                     ELVYQYIDNTESRKSSNGAYVLPVDNPVISSGFGWRSDPFGGGTAFHRGLDFANPFGS
                     NIKAIAAGKVITAGPHYSWGNHIVIQHENGQVSLYAHLNKILATVGQTVKQGQTIGQL
                     GSTGNSTGPHLHLEIAKSSDLSQANLIDPAVALGLK"
     gene            47557..48198
                     /locus_tag="ACHALG_000313"
     CDS             47557..48198
                     /locus_tag="ACHALG_000313"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2585756.1"
                     /translation="MERKKSVILVILVSVMSFIAGSLIALHYLQPVLKERENNTLLSE
                     QKANQETINSYEKENKLLREELDSQEVNLPKDTRSEVEFKNIEKVAKAFTDGFYTNNS
                     DNIQEKMDQIQEYLTPKAKGKIVPYWVESNDKLTVEQSKISEKYYVNLENVLGEATVT
                     GFLYVSTSYEGDKPFTVRYLIQMDLKKNENNVWQVNELRETVTTNDVPERFYD"
     gene            48214..49173
                     /locus_tag="ACHALG_000314"
     CDS             48214..49173
                     /locus_tag="ACHALG_000314"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2585757.1"
                     /translation="MKKMNELIERIETFREDFQKGHFQKSKVKQKRAKLSPIIYRGMF
                     LLTIVGTLFFFSSRLLFEDDRPIIDSGIGGKSSQTIGSDTVQIYQRTYNPKTNYMEVI
                     FLTSRNETSVNRAYQAFVGSVKEGNKGTALASKLEPLYDNYYLLTIPEVPQKWTTLLI
                     DFGYSEKEINVNPTYNQEGEMVFEDTEEVNQTVFYIDYRKVKNSSQLKKKKLNDYVIE
                     LTEKDIEKAIEERKNLQDSIQKIETEIEKTKSKILENETSMEYLTAGEKEEIENANKQ
                     LSSQIDSYSNAIEEAQKQIEVIEERRQKLIDRNQSAQVETENK"
     gene            49506..49775
                     /locus_tag="ACHALG_000315"
     CDS             49506..49775
                     /locus_tag="ACHALG_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008381265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2585758.1"
                     /translation="MSRTKPLQIRDVDEEIHQRLVKIAKKKGYKSRDDMLREVLTQLA
                     YDEFQLDSEIRYRQFIEKQKQFMEWLAITVVEKSYSEIEKEPFTE"
     gene            49794..50252
                     /locus_tag="ACHALG_000316"
     CDS             49794..50252
                     /locus_tag="ACHALG_000316"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2585759.1"
                     /translation="MEQENLEQENLTSDPSKDKLEEEKWRKKNYWVKESTIERIRYWN
                     EVRHDKNEGYTLEALLDFYERNHAQEQQALEEILTLQRQQMTIIREAEMNSKVNQQLL
                     NTMIWFHEIESFQPTDSVPLIEAKKYLQELQESQIKTAHHRKKYSQVGDE"
     gene            50252..52309
                     /gene="mobP2"
                     /locus_tag="ACHALG_000317"
     CDS             50252..52309
                     /gene="mobP2"
                     /locus_tag="ACHALG_000317"
                     /inference="COORDINATES: protein motif:HMM:NF037847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobP2 family relaxase"
                     /protein_id="HFN2585760.1"
                     /translation="MARQTAGIIYSAAFVISSAQAFKEFIDYMNRSAAVRNENFDKYN
                     AFSAQNQADSYNSIEGGNETMFSTYNDYMSNPEKTSSSFTRHYDQAPIEVMNHLKDYF
                     TQAQENQSPLWQMVFSFKNEWLVEHGLMNEKTNRVDTVSLIDAARKAVTDLEKKEGLE
                     GEWSGAIHYNTKHIHVHIGYVEKEPTREWGYYEHPTDPEKTGWQYMAKFKQSAIKSTR
                     RTFVNTLLKNESQLKELSQSIRQQVALAKENRSEFLAGRYEMLATDLLEALPDNKYFW
                     KYGFAQRHGFKEPLDRMVNLFLNTDGKETMEKVIRLLKPISDEYESAYGNPRNAPTYY
                     ENQLYGKNGLYAQIGNQILHEVRTFVKSQQMQKAVGNMTAEEFEALQKEQAVLPIDLG
                     SPTEQDFIVPEMEDVFHLLNEIPPPLDHEEVEQPVSHSILKTEGNSDSVERTKALSEA
                     FELMKQHFDKRDESLDQRHKGIQYKLNQENANREDTIERRAMYIHAVDSETDFVHYEP
                     TSIQKHKTLDEEFVSAKEQKSSESEEGNEQHNLDRFSKKNRTMILSQNPGATFVYGPQ
                     QWKLNGRKVRDDEINQPLVIIAPYKIEDTVVFEEICVYNISQTEAYKPTAAELEELLK
                     KETAKGKSMAPRSVSRFQDRRNEMEMRKMVQSLRRSCENSLQHYLNERAYRELQYESQ
                     SNI"
     gene            52374..52757
                     /locus_tag="ACHALG_000318"
     CDS             52374..52757
                     /locus_tag="ACHALG_000318"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2585761.1"
                     /translation="MVQTDTGDQGIDVRKEQQKIVAYFEKDPTVNLWVTPDENGQLYV
                     VCESEDDFLKTYLYEDVENFIDANPEKHGSSFQLFEWIDHSVSEFEASEDIDHGDEEM
                     IIEEKQMIDKSKKKRKEKAQGLEMT"
     gene            52851..53444
                     /locus_tag="ACHALG_000319"
     CDS             52851..53444
                     /locus_tag="ACHALG_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008381274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HFN2585762.1"
                     /translation="MKKVVYELLEQYNGTLAMKDAKKAGVSPVTIKRMVDRGELDREY
                     PGFFTLPGQFADELLIAQKKYERGIISHITALDLYDLTDMIPRKIDMTVPYGYHVSEK
                     GLTEFAVDLHFTKPEYYSVGRTKVRSRYGNTLVAYDVERTLCDIWNPWYKVTDEIRIK
                     AIKYYMNSKVRDLSKLRMYQRILPVDKEMRSYIMALH"
     gene            53537..54295
                     /locus_tag="ACHALG_000320"
     CDS             53537..54295
                     /locus_tag="ACHALG_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008381275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFN2585763.1"
                     /translation="MNYGFYDEYSIARNQFHNRKFPMEYLDDILVRLAYHSSAIEGNT
                     ISLPQTVSIILHNTVPERTSLRELYEIENHRGAFALMLEKVQDKQPLSISLVKDIHER
                     LMDKLIVDRGQFKSYENAILGADFDTASPQETPVLMQQLVDNQNYRLEIADTDVEKIE
                     AIMDTHIQFERIHPFSDGNGRTGRLVMNYSLLNEGFPPVIIQTEDKAEYLELLSEQNV
                     KGLTRYAMEQMVTEEKRQAKFIAKEKSQIQELDF"
     gene            54390..54560
                     /locus_tag="ACHALG_000321"
     CDS             54390..54560
                     /locus_tag="ACHALG_000321"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFN2585764.1"
                     /translation="MKAVKTRIIGNSLVISLPKELQIKENQYFYCHKKENGIIELVPK
                     IENPLKQISDEK"
     gene            54550..54870
                     /locus_tag="ACHALG_000322"
     CDS             54550..54870
                     /locus_tag="ACHALG_000322"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2585765.1"
                     /translation="MKSNWSQRNILSDTEGQMYLILSANVFYEMYERVIVCKVTDAAT
                     EAPLEVKISLEPSKHSMIVDCSEFFTINPSADLYTLYGTCDEGEFLLVAQTFLAIFDF
                     LFFD"
     gene            55523..55861
                     /locus_tag="ACHALG_000323"
     CDS             55523..55861
                     /locus_tag="ACHALG_000323"
                     /inference="COORDINATES: protein motif:HMM:NF025561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnpV protein"
                     /protein_id="HFN2585766.1"
                     /translation="MKLTYQENQNGQLIPQVEYPENQPLGRFGSIAVEKLKEEQPTEY
                     QMKLMQGELMTYGHTIDKKVWERVAVLAEQMEQQNPLTLEEQQNLETATKIRNNYREQ
                     AIELAMSELL"
     gene            58768..61410
                     /locus_tag="ACHALG_000324"
     CDS             58768..61410
                     /locus_tag="ACHALG_000324"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HFN2585767.1"
                     /translation="MNHSQVLEELQGIYEERFNRLVPRAYDGSYLEFEGLSEQIELRP
                     HQKNMVARIIQNGRGLMAHVVGAGKTLTMISAGMMMKTHGLIKKAMYVVPNHLTGDFG
                     TELLRFYPSKKVLVTTKKDFEKANRQQFVSRIAVGDYDAVIIGHSQFEKIALSPERQR
                     NMLKAEINQVSEAIAAYQMQNDVDSWSIKQMVSFEKRLNERLAKLNKQDKKDDLIYFE
                     DLGVDFLFVDEAHLYKNLYSYTKLSNVAGVNASNSLRSSDMEMKVKYMLEENQGRGVV
                     FATGTPISNSMSEMYTMQKYLQPDVLKDFGVYHFDAWASTFGEIVSSLEITPEGSGYQ
                     MKNRFSKFHNLPELMSMFNLIADIQTKDMLNLPVPAIKGEKAQIIVTEPITYQKDKMD
                     QLAMRAEKIRNREVSPEVDNMLKITNEAKLMALDPRLLDDYDESKYDRQELKQTKIAR
                     AAEKVFSIWLETKEQRSTQLIFSDSGTPKPGKFNVYDEIKRQLIEKNIPENEVAFIHD
                     AKTEAQRDALFEKVREGEVRILIGSTGKVGTGTNIQDKLIAAHHVDCPWRPSDIEQRD
                     GRIVRQGNENKEVQIYRYVAKGTFDSFLWQIQEQKLTYISQVMTGKAITRTVDEMSET
                     VLDASEIKAVATGNPLLADKMRLDNEITKLRMLQSSYLNERELLKRSIDQIYPSMIKS
                     GEQHMVTLQEDIQRYKDNDPDEFSIVIDGKEYTERSKAGEVFESATFLYMRSPDFVEI
                     GEYKGFSFSVRKSELSYGSLQVRLHGKGHYDLLVDPSTQIGSIRRIENKINQLPEMLQ
                     EAAEEVQETKEKMESAKEQIKQPFSHAQELQEKVLRQQEIQSEIESSLNKSDTQSIAD
                     EIEETEKSQVAYSKEDYLNEEREM"
     gene            complement(61498..61701)
                     /locus_tag="ACHALG_000325"
     CDS             complement(61498..61701)
                     /locus_tag="ACHALG_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004897187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2585768.1"
                     /translation="MDNSSHKTSEALRQAVKRYEQKNAKAKQYRNKKSATKSFILNLA
                     TEEDLQLVEEWLKERENQGNHES"
     gene            61838..62047
                     /locus_tag="ACHALG_000326"
     CDS             61838..62047
                     /locus_tag="ACHALG_000326"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2585769.1"
                     /translation="MNMMLKNKEYQVRFEEYADNNNVAIMVVKPKKGRMREEELVTTA
                     TVNTDTAIKPDFVAIKGWSENTGIE"
     gene            62254..62433
                     /locus_tag="ACHALG_000327"
     CDS             62254..62433
                     /locus_tag="ACHALG_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2585770.1"
                     /translation="MVIARGNKRLPVTLNVHRQKSLSQLKKKYSKSESKLLCIALDML
                     VEQEKAGFEIPKLRE"
     gene            62539..63405
                     /locus_tag="ACHALG_000328"
     CDS             62539..63405
                     /locus_tag="ACHALG_000328"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010013097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFN2585771.1"
                     /translation="MITAKSYFSGAGGMDLGMVEAGINILESFEIDKKAVATLKMNFD
                     HTITQCDTTQITVLDQQDADVYIATYPCTKYSNIADISGTRTGDDLFLHFFRHIALSK
                     PEAYVIENVPGMRKFQVVMECMTKLPDYYVRVECPVNANWWLPQERKRLIVIGTRKPF
                     NNLDYPLAIDQTPITVGDIMESDPEIHIPEYVYKRLNGGYRDKPIITPRNGKAPTCVA
                     HYAKDRSTRLVDDGKRIRPYSVLEYQRLQGFPDWFKFEGTDNDAYRQIGNAVPVDMGR
                     WIGQQLKKYFES"
     gene            63644..63835
                     /locus_tag="ACHALG_000329"
     CDS             63644..63835
                     /locus_tag="ACHALG_000329"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2585772.1"
                     /translation="METLKKALEKRKIECRVETPDTVYCGSPKDLLLAISENELQLVV
                     INKRLESLFGDKRWDIEVG"
     gene            63871..64167
                     /locus_tag="ACHALG_000330"
     CDS             63871..64167
                     /locus_tag="ACHALG_000330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2585773.1"
                     /translation="MSKYETKESKKLKKELREIDVKGVVTHIVFSLIVCMFLGSFYYF
                     FRWKIIGALLLFFTGYGILGIVLVAISLFVQKWGLKKIKEKEESDYFKNDDLDF"
     gene            64232..64489
                     /locus_tag="ACHALG_000331"
     CDS             64232..64489
                     /locus_tag="ACHALG_000331"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2585774.1"
                     /translation="MELISAEGKERIKSVYRDVCIAENAYRNDVGGHNGNFEQRKIAI
                     DDVFQSLGMIHEARYIEEEISNQTWTRSRHIDGRIKYGLVQ"
     gene            complement(65129..65524)
                     /locus_tag="ACHALG_000332"
     CDS             complement(65129..65524)
                     /locus_tag="ACHALG_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2585775.1"
                     /translation="MKNKGQIWYIGYIVSLFLIVIILVTDFPKMVDIGLAILFSTVFG
                     VSHVQILHDKMIKNDADYKINVMDERNIIIKEKAGNITNMFNTILLGVATVIFICLDY
                     IIPAIIVGSIIAIQPIILIIISSVLEKKM"
     gene            complement(65514..65726)
                     /locus_tag="ACHALG_000333"
     CDS             complement(65514..65726)
                     /locus_tag="ACHALG_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN2585776.1"
                     /translation="MKNRLEELRKQRGIKQEDLASALEVSRQTIGSLENGRYNPSIIL
                     AFKIARYFQMSIEEIFIYEEKSEDEK"
     gene            66053..66715
                     /locus_tag="ACHALG_000334"
                     /pseudo
     CDS             66053..66715
                     /locus_tag="ACHALG_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002393257.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
     gene            66741..66986
                     /locus_tag="ACHALG_000335"
     CDS             66741..66986
                     /locus_tag="ACHALG_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2585777.1"
                     /translation="MSQERKCYGGQWKVPITLYNKRLYWPPSWIECNCGELAKQVRER
                     KGDHLYANGRYLCNSCHREYEMVYGKNQFILVNENEA"
     gene            66976..67293
                     /locus_tag="ACHALG_000336"
     CDS             66976..67293
                     /locus_tag="ACHALG_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016620977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2585778.1"
                     /translation="MKPEGSLISFENKRKKQWTVIVLSRFSKELIGFLSVTAATENLC
                     FTQKKEELGLFTEAEANEILDSINALKDEEDVIYKTNEKTGTADWISGKYFDFKKAKV
                     QFE"
     gene            67332..67532
                     /locus_tag="ACHALG_000337"
     CDS             67332..67532
                     /locus_tag="ACHALG_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016620725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2585779.1"
                     /translation="MTDHEKIAKLLNKKFSAMDFTKIERLEDGIWHLVDKTEEMFVHF
                     EGNVAKIRSIVNSIEYEKSIEL"
     gene            67644..68003
                     /locus_tag="ACHALG_000338"
     CDS             67644..68003
                     /locus_tag="ACHALG_000338"
                     /inference="COORDINATES: protein motif:HMM:NF017470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFN2585780.1"
                     /translation="MSIFNYILSNITKWNGTTSRGKYWQIFFIVFIFGFILEVISRQM
                     TGISLIQQSFYFKYTSLLHFILLLLFTLLNLMLWVFQIRRLNDLDLTRWLVLVNLIPI
                     AGPTIMTLLMLKKGKTL"
     gene            complement(68172..68486)
                     /locus_tag="ACHALG_000339"
     CDS             complement(68172..68486)
                     /locus_tag="ACHALG_000339"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2585781.1"
                     /translation="MKKTINVALLCLLLSSVALSTISSTLPLGTTVAYAQDDGLGTWA
                     EFALNQIPNAKIRKEARSKYYSLQKSHNQCIAKAFLSGVAFKGLTIQAAIQGAYNSLS
                     CK"
     gene            68781..68945
                     /locus_tag="ACHALG_000340"
     CDS             68781..68945
                     /locus_tag="ACHALG_000340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2585782.1"
                     /translation="MNIDNEKQVFACSAAKKSNTLPTAPMNVFACSAAKKSNTLPTEP
                     LKSVSWGEKQ"
     gene            69097..70209
                     /locus_tag="ACHALG_000341"
     CDS             69097..70209
                     /locus_tag="ACHALG_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004047540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFN2585783.1"
                     /translation="MKNIIYIGMDVHKETFNLCALHGLTGEILGETKCASDVSLVMKF
                     VRKLKKRHGEDTAILAGYEAGCLGYSLYNQLKSQGVECDILAPTTMYSSIKNKMVNND
                     RSDAKMIASNLVNGTYKSVYVPQEDHVEVKEYIRMVNDFKTSLKKIKQQIKALVLRHG
                     YKYPGKSSWTLAYMKWLKSLDLKGGVKETLDEYLLQYDTLIDKIQRFSERLEELSHRD
                     SYEEPVAALRCLKGIDTTAAMMVHVEISDFTRFPTAKAFTAYLGLTPSEQSSGEKISR
                     STITKQGNSTVRSTLVECANALVKGTIGIKSKRVKARQKGQESQVIYYADQAVERLQR
                     KYHRMIYQGKPRNVAITAVARELACFIWGIETQKMM"
     gene            70742..71710
                     /locus_tag="ACHALG_000342"
     CDS             70742..71710
                     /locus_tag="ACHALG_000342"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFN2585784.1"
                     /translation="MTYLCNLYCPYCYAPRNTFFVSQENNVAFLKKLVELNAKNIVLI
                     GGEPMMHPKLDKIIDEIISYGFTSISIITNGTILKEKVLEKIVKYNIDIQISVDGYTE
                     ETNAPTRGKGSLNRVLKNIDILKQNNVNYSVMQVLTKETIKNSEEFSEFFSKKGIPHG
                     FFLVKETDDTSRPSLKSIYKLYDFLYSRYGDVNKVFDCVKSSDQMQLSSTGFPITHCG
                     AGITTLSIDPLGNVYPCVKLHNADFKITNLTRSTAVVDIKNNRNRVLENELVDSVDKC
                     KNCSIKYICGGGCRAEEYYVSKNKQSYSQCDLQKGNLTYFLENINE"
     gene            71703..72902
                     /locus_tag="ACHALG_000343"
     CDS             71703..72902
                     /locus_tag="ACHALG_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012898298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFN2585785.1"
                     /translation="MNKKIVSFLCLNVLVLFSTTLFQLNILWEIFSQQEELKNLVMII
                     GSSFILQDGMSLVVGIIVDSYSKKKVFFFSIFGFIITVAASYFINMDIQWAVFYLIFT
                     AVNTLFLRCLVSAAAEMLTTDEFMKYDGITGLFNQILIIISNVISGILIKFVSVEAIY
                     FTIIFLLILALFLIFFLPVKDIQGNESIKDNEEIKEKIEGSLLLFIKKNIFKDKKIVV
                     FICLLFLLNLDYGYIPNILPYYMFKYTSQTSPIFLSLLKSSVNLGEIVASFFVIHYRK
                     KVSKMTKIGLLGSAICFMLLPFSNQMILVNFFVLSFYGFFDTLTQPLFSYFVSSIEHK
                     NRGKILGIVDCVVYLAVPIGMSLGNFISQYGMLILSLSISIVFFCSYILLVKTKTYKS
                     IDLAGDL"
     gene            72902..73594
                     /locus_tag="ACHALG_000344"
     CDS             72902..73594
                     /locus_tag="ACHALG_000344"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN2585786.1"
                     /translation="MNLIQEKFLQNELVWLKSLSCEIHSTENYIIVKSDFSQSEDFNF
                     IFPRKVVQEKDLYMIMDQYKIKYLKIATPTLIDLKLTSAMTCLKTYDLNFNANASLSN
                     FRKLCSKKKGIRLSANIPWKYNDLPNRIFSSNVVPFTICFNQYEVGQFCLVILKSNIG
                     IYDLQIYKEFQNQGFGNEFLEELLLVKNELEIGNIFIQTWNQNIIAKQLYSSKGFNVI
                     GKYQYYTYTEEC"
     gene            73770..74369
                     /locus_tag="ACHALG_000345"
     CDS             73770..74369
                     /locus_tag="ACHALG_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016614778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFN2585787.1"
                     /translation="MIFLKIGYARVSTGLQNLDLQEDSLKKFGCEKIFTDHMSGAKNN
                     RPGLETAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNEWSVSFHSLQENITMDKS
                     SSTGQLMFHLFAAFAEFERNLILERSAAGREAARARGRYGGRPEKFTDQDLKLMKTLV
                     DSGTPIKTIAERWQVSRTTIYRYLEKMEKTESEEEEIIQ"
     gene            74391..74693
                     /locus_tag="ACHALG_000346"
     CDS             74391..74693
                     /locus_tag="ACHALG_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016609790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2585788.1"
                     /translation="MKEKEIHLAIVKLTEDPEWIHCAEIVAYVQELSKKFSTTRQSPI
                     NYNSINGIKVVYSNGSHRIYPSLKQCALEEKLYNGTIQRVIRMKSKTSDGRQFIVI"
     gene            74801..74995
                     /locus_tag="ACHALG_000347"
     CDS             74801..74995
                     /locus_tag="ACHALG_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2585789.1"
                     /translation="MEEKTIFEKRWQLASSDQRARFDKLLSSYPTIEWTYKEKKYLLW
                     LCQLDVDTFETIEIIFEKIK"
     gene            75035..75460
                     /locus_tag="ACHALG_000348"
                     /pseudo
     CDS             75035..75460
                     /locus_tag="ACHALG_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295591.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(75493..75948)
                     /locus_tag="ACHALG_000349"
     CDS             complement(75493..75948)
                     /locus_tag="ACHALG_000349"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2585790.1"
                     /translation="MYAIDTFYSKERQYLKAEQQKITLLYEQFEATYQRHPDYRYLSQ
                     CLTQNILKKEHLFASHEKNFMSSEHFANIYTDQLKQKHLCTFEQFGHDTFYILEREHY
                     RAITHYNEQNQTIEGYPELKIQNNCFLQQQLLKQLTNEFHEIYQNYQIN"
     gene            complement(76102..76392)
                     /locus_tag="ACHALG_000350"
     CDS             complement(76102..76392)
                     /locus_tag="ACHALG_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2585791.1"
                     /translation="MTNTNSIPIVDCYDRNTNDYLGSFEQTDENIINYIAGRSPFQSV
                     YLVEQRTDTLILTTIGNFLDQVPNQQWLQQILPLLIAKQTGERPIEPVPMLS"
     gene            complement(76445..76696)
                     /locus_tag="ACHALG_000351"
     CDS             complement(76445..76696)
                     /locus_tag="ACHALG_000351"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2585792.1"
                     /translation="MKLTEKNAWFRPVKKQTIDIHTSMSGTTAPIPNKFIDYPLARTK
                     DSIVRKRINLNHPNELYVNRSWDTFYRYDGQPVYESSYY"
     gene            77739..77927
                     /locus_tag="ACHALG_000352"
     CDS             77739..77927
                     /locus_tag="ACHALG_000352"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2585793.1"
                     /translation="MIDDVVRDWRTLDAYLEQTGYLCEYADGECWKYRLRTSNQIDFY
                     IRKIVVPADDKKTTTFSK"
     gene            78405..78866
                     /locus_tag="ACHALG_000353"
     CDS             78405..78866
                     /locus_tag="ACHALG_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2585794.1"
                     /translation="MKKVFIVLIAGLTIIGSGLFIGNLFFSKEEEKEPSTIATTEELK
                     PELFLDIEILESNQEESQQETAINELYRGELDFQHADPTKPYLITVQFIDKEKREFFG
                     PDKQPLRLEKEVTLGKEQGTVFLVVKGNKPMLAHIQKLKDAQVSASLKEKE"
     gene            78886..79200
                     /locus_tag="ACHALG_000354"
     CDS             78886..79200
                     /locus_tag="ACHALG_000354"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2585795.1"
                     /translation="MDDEMNRSVYSYKILISKEAVREKFDVYSLKNHIIFQLHGYTYN
                     SYDRINYTINLSLREMVVTMTKKDGSTFSANEWRFFDRILPEFFEKLEVAFIQEKEIM
                     EE"
     gene            79921..80109
                     /locus_tag="ACHALG_000355"
     CDS             79921..80109
                     /locus_tag="ACHALG_000355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2585796.1"
                     /translation="MNLRVSQCSKFLYLVIGLVVTVSILFGVSIKGHADEKTPDVENS
                     VYDMDENQILKIMNEYIY"
     gene            80093..80500
                     /locus_tag="ACHALG_000356"
     CDS             80093..80500
                     /locus_tag="ACHALG_000356"
                     /inference="COORDINATES: protein motif:HMM:NF012297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004623 - phospholipase A2 activity
                     [Evidence IEA];
                     GO_process: GO:0006644 - phospholipid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0050482 - arachidonic acid secretion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A2 family protein"
                     /protein_id="HFN2585797.1"
                     /translation="MNIYINENGIREFDLNRAIANNLPDEILEIGNTMNNIVLEVEKE
                     KKNDNLTVRIVKRATFLVYGNYCGYGNKGKGKMPIDDLDTACMYHDYCYVHGGNNTDC
                     NRKFADRLRAVIKSAKKFSYKYNVAVGALAIFG"
     gene            80509..80775
                     /locus_tag="ACHALG_000357"
     CDS             80509..80775
                     /locus_tag="ACHALG_000357"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2585798.1"
                     /translation="MKKYLISINIFLLLFSAALIICQLFFYQAKGIPPIMFTPVTGIL
                     GIIMALFLPTNNHLRKIFLIVNSSITLIVFIYFLAVAINPDFFV"
BASE COUNT        27957 a        13012 c        16924 g        22883 t
ORIGIN      
        1 aaatagttaa cttttctttg actttcaaca tctgagtgcg tgtcgtatag gaaccgttgc
       61 ggataatacg aaccgatcga cagtgttcac aataggaaga agagtcatcc aattttcctt
      121 taataaactg cgcttgaacg cctttatatt ctttttcaat cagccattct tcatcaaaaa
      181 ataaatgttt atctgttaat ccgagtaatt ttctagtatg attatccatt gagagactcc
      241 tttggtagga tgacgttttg gtcgactaaa ttttaccaaa agtgagtctc tttttgagtt
      301 gaaacgaaga aaaagtgtta gtagaaaatc gccttcgatt ttccaccaac acaaaatatt
      361 atagaaccct ttttttttgc tatatgacag gaatgaaagg tgtttgcaag cttaatgtga
      421 ggaacacttt ccatatctgt tctatactgg ataggagaag tttggaaata tccagaaaac
      481 gaaatcaatc gaggtagcca catgaagaat aaatggataa atcgaagtaa aaaaaagaaa
      541 gtaacacaaa gatcatcaga taaagtcatc cgtctttttc catcgtataa agaaggatta
      601 acggcagatc aagtcaagga acgaatagaa aaaggtgcag caaataattc agtcgatccc
      661 acattcaaaa caaatcagca aatcgtattg gaaaacatat ttacgtattt caatttgatc
      721 tttcttatct tagccatttt gctttgttta gtagaatcct ataagaactt gactttttta
      781 ccagttatca tagccaatac aggaatcgcc atttttcagg aaattcgatc caaaaagatc
      841 ttggacgaac taaatgtttt aaatacagca aaagtcaatg tcatacgaga cgggatagaa
      901 caaacaatag atatagaaga ccttgtaata gatgacattg ttattttaga gactggatat
      961 caaattccag cagatgcaga ggtcgtggaa gggaaactgc aagttaatga ggctttattg
     1021 acaggcgaag cagatgaaat aaccaaagaa ataggtgatc cattaatgtc cggcagtttc
     1081 attgtttccg gaaaagctta cgcccgatta gacaaagttg gagcagattc gtatatttcc
     1141 cagctgacag tcaaagccaa gacaatggga gaaggagaac agtcggagat gattgcttct
     1201 cttaatcaat tgatcaaatg gattgggatc atcattattc ctatcggtat cctgcttttt
     1261 tctcaaagtt acttttttaa tggaaacacg atcaaagaaa gtgtcgttgc aatggaagca
     1321 gcattgatcg gtatgatacc tgaaggactg tacttgctga cgacgattgc attagcatta
     1381 agtgcgacta aactagcaaa gcaaagagtt cttttacaca atatgaaaag cattgagaca
     1441 ttggcaaggg taaatgtcct ttgtgtagat aaaacaggaa cgatcacaga aaataaaatg
     1501 tctgttcaaa aaatgatcgt ctctaaaaaa caagaaataa tcagtcagtc agatcaattg
     1561 gaagcatgca ttgctgacta tgcaaaagcg atggcaggag acaatgcaac aatggaagct
     1621 gttaaagcat attttacaaa aaccactgat caatcctatc accatgttat tcctttttct
     1681 tctgtccaaa aattcagcag tgtagcgttg gcagacaagg tgtatgtttt aggagcgccc
     1741 gaaatggttt tgcgcgatca attgccggaa tatgcggaag agtttgtttc tttcgctgaa
     1801 aaaggctatc gcgtgttggt ctttggtgtg tacgatggga ttcttgaaga accagtattg
     1861 acagaagcag tcacaccatt aggctatatt ctgattgcta atcctatcag aaaagaagca
     1921 agagccacat ttgattactt caaaaaacag caagtagcca tcaaagtgat ttctggagat
     1981 aatccgctca ccgtatccaa tgtagctgtc caagcaggga tttcacatgc tgatcagtac
     2041 gttgatgcat ctaaattatc agaagaagaa tatgaagcag ctgtcgaaaa atatacagtt
     2101 tttggacgcg tcaagccaga acaaaagaaa atcttcgttc aattgataaa gcgtaacaat
     2161 acagtagcta tgacaggtga tggcgtgaat gatattctag ccatgaaaga agcagactgc
     2221 agtatagcga tggcttccgg aaatgaagcc gccatgcagg cctcacaagt ggtcttactt
     2281 gattctgact tttcacgcat gccagaagtt gttgcagaag gacggcgcgt tgtgaacaat
     2341 attgagcgat cagccagctt gtttttagtg aagaatattt tctccttttt gctctcactg
     2401 ttttcagtga tctttgctct gacctatcct ttagaaccat cacaaatcac attgattagt
     2461 ttgtttacta tcggacttcc atctttcttg cttgctctag aagaaaacaa aaagcgaatc
     2521 agagggaaat tcatcatgaa tgtgatggaa aaagctgtgc caggtggact aacagatatg
     2581 atcgtagtcg gtgcgttggt gatctgcggg gtgactttga atctaaataa aacagatgtt
     2641 tctacagcat ctaccatgct tttgattgca gtagggttcc tcgttctcta taaaatctgt
     2701 tcaccgctga ataaattcag aagtcagatc attcttttct gtgctagcgg taactttttt
     2761 tcggtcatct ttttgcataa gctgttttca atcaccggta tctcagctgt atcacttttg
     2821 ctgttgagca ttctattttt ctcggcagat tcgattttta ggcatttgac cacattggta
     2881 gaatatttgt ttgaacgtaa aaatgaaaaa gagcaaaagc cgtttagtcg cgtagctaaa
     2941 aaatttctat cgatatttaa acgagaaaat tgacgaaaag tatctcaaaa aagagtaaaa
     3001 aagaacaacc actcgtgtat ctttcgaagc aggatgagtc atccaacttt aaaacgcacg
     3061 agcagttgct cttttttaca ttttcggaat ggtaacggtg aaagtcgaac ctgttccaag
     3121 atgactgtct acagtaatcg taccattaag gagttgcacg tagtctttta caatcgctaa
     3181 ccctaatccg gtcccgcctg aatgtcggct tcgcgccttg tctacgcggt aaaaacgctc
     3241 aaagatccgt tcctgatcct caatgtcgat acctatacca aagtcttgga cagaaaacga
     3301 tagatcatct gttgcctgat agcggataat aatctcgctg tcgctttttg aatattggac
     3361 agcattttca atcagatttt tgataattgg ataaaggata tctgttttag tagtaaatgg
     3421 ttcgttcttt ccaatcaatc gaatcgtcaa ccgtttcttt tcgatgattg gctgataatt
     3481 ttgaacgatt tgctggaaat aaggttctgt ataaattgtc tggatctcgt aagaattctc
     3541 gccatctttt gacagctgga tgatttcccg aatcagttta tctaaccgga tcgcatcttt
     3601 ttgcatgatc tcaagaaaag aagtcagagt ttgcgggtca tcttttgcac catccagtaa
     3661 agtctcggta aaaccaatca gtgaagtcac aggagttttt aattcgtgag agacgttgcc
     3721 aacgaaatcc ttttgtagct tttctaaacg tcgcactttc gttaagtcat acgccacccc
     3781 tagaatctgt ccggtacctt ctgaattagt aaagtagcgc aaactcaagt ctaatgtctg
     3841 ccgaccttct gtaagggtga tctcctgatg gatcagcgga tgctcaggag tcacttgatg
     3901 aatcagctga atgagttttg gctcttgaat gacttctgtg taccgctgtt caggttgata
     3961 gcttaccacg tttaaatgta gctgcatctt tggattaagc agtagtaaac gagaatcagc
     4021 atcatcaatc aggaatacgc caatcatcag ttcgttcagt aacgtatata attgctcttc
     4081 tgtggtagta cacgcccgat acatcttact catttgttcg ctcaacgcat taatcgtctg
     4141 atacaattct tcccattgat cgggtgtttg catgattact tccgcctgat taggattttg
     4201 caccattttt tttagtacgg gcagcacggt tttcaatggc tggttttttt gatagatcag
     4261 ataatagatc attcctgcaa gaacaaggaa gaaagacaga aagaagaaaa agacccaacg
     4321 gcgaaaactc tcagttctcg gtaaaaaaac gcttgtcggt tctgcgatac gtaaaatggc
     4381 ttctaactgg ccactttttt tgactggtaa tgcgacatat aaaagatcct cattcaaagt
     4441 agtacttttg cgcaaagcgc tacctaggtt cccaccattc aatacggctt tgatttcggg
     4501 acgggagttt cgctgttcat tcaatgtttc attgtttgtg tcaaataaga tctttccagt
     4561 agcatccatt aaagtaatcc gttcgttcga ctgatggacg aattcttcca aagcagtttt
     4621 gttttgcgct gcttggaggt tttcgacatc taattgccgg atcagcagtt gtgctttttt
     4681 tgtcaggtag ctttcttgct gttccacgat ctgctgttga aaataatggg aaatcaattg
     4741 ccagccacca aaaaacaaac taactaaaag aataagaaag aagagacgtt cataaatttt
     4801 tttccatttc atcgtttggg ttcctgaaac ttatagccaa atcctctaat agtcaccaag
     4861 tactttgggt gttttggatc tcgctcgatt ttttctcgca aatgactaac gtgtacatct
     4921 acgattcggc tttgtcctgc gaagtcaaaa ttccaaatgc ggtctagcaa tgtgtctcgg
     4981 tcgatcacac ggtcttttcg tttcatgaaa taaacaagta attcaaattc tttcggtgtt
     5041 agttcaatca gatggtcgtc tacggtcact tgataattgg tcaaatcagc agtgatttgt
     5101 ccaatcgata gatattcagg ctcagcttca tcttttgatt gcttacgagg ttcaattcgt
     5161 ctgaatattg ctttcatacg ggctaatact tcgcgaggac taaatggttt ggtcaaatag
     5221 tcgtcagcac ctatttctag gccaatgatc cgatccactt ggtcatcttt agccgtcagc
     5281 atcaaaatcg gtgtttcatt tttttcttga cgcagccgct gagtgatctc catgccatcc
     5341 atcgttggaa gcatcacatc taaaatgatg aaatcaaatg attgttctaa tgctaattct
     5401 aatccttcgc ctccatctgt ggcactggtt accttgtagc cttctttttc caaattgaac
     5461 gtcaagagcg ttacaataga aggttcatca tcaacaacta gtattttttt cattcaaatc
     5521 tcctttaaat aggtttcatt ctgtttatat tttagcatag aatcagtaca aagggctatt
     5581 ttacaaaaga aataaatggt ataataggaa ggcactttgc tgaatagaaa ggaagagcag
     5641 tcaatgattg gaatcagtgc atgtttaggc ggtctggcct gtcgctatga tggacagaaa
     5701 aaagaagtac cagaattgat ggaactagtt gaaactggag aagctgtcat ggtctgtcca
     5761 gaagtgatcg gcggattacc aattcctaga gatcccgctg aaatcattgg tggaaatgga
     5821 ttcgatgtgt ggaaagatca agcaaaagta tggacgacaa gcggcgaaga tgtaacggaa
     5881 gcgtacaaaa atggcgcgat caaagcctat caaaaattgc aggaaaaaca cattgatcta
     5941 gtcatcatga aggaaaaaag cccttcatgc ggaacccatt cgatttatga cggaagtttt
     6001 tccggaataa aaaaaaatgg tgccggagtg gctactgctt attttattca gcaaaaaatg
     6061 actgtgctct ctgatgaaga atggctgaaa actagaggtg agcagaatgg aaatcgaaaa
     6121 gaccaatcga atgaacgcgc tctttgaatt ttattcgacc ttattgacag aaaaacaaat
     6181 gaactacatg gaattatatt atgcagatga tttttcatta ggtgagatcg cggaagaata
     6241 cgaagtaagc cgacaagcag tatatgacaa tatcaagcgg acaagcaaga tcttggagga
     6301 ttatgaaaaa aaactccatc ttttttctaa ttatattgtt cgagaacagg tattggaaaa
     6361 tatgactgca tatattacag ataagtaccc ggaagataaa aaactacttg aatatgtaca
     6421 gcaaattcaa gcaatcgaag aataaataaa aggaatgaag aaaatatggc atttgaaagc
     6481 ttaactgaac gtctccagca ggcgatggga aaaataagaa aaaaaggaaa agtatcagaa
     6541 gctgacgtaa aagaaatgat gcgggaaatc cgccttgcct tactagaagc cgatgttaac
     6601 ttgcaagtag tcaaagattt tactaagcgc gtacgcgaac gtgcggtagg ggcagaagtg
     6661 ctggaaagtt tatcacctgc acaacaaatc gtaaaaatcg ttgatgaaga attgacgatc
     6721 acacttggtt ctgaaacagc agagttaaat aaatcaccaa aaataccaac tgttatcatg
     6781 atggctggtc ttcaaggggc aggtaaaacg acctttactg gtaaattagc caattattta
     6841 aagaaaaatg aaaatgctcg tccgctccta atcgcagggg acgtatatcg accagctgcg
     6901 atcgaccaat tgaaagtatt aggtcaacag ctagacgttc ctgtatttga tatgggaact
     6961 gaagtcagcc cggtagagat cgtccgtcaa gggatggagt tagcaaaaga aaagaaaaac
     7021 gactatgtat tgatcgatac ggcaggacgt ctgcatatcg atgaaacatt gatggatgag
     7081 ttgaaacaaa tcaaagaatt gacgcaacca aatgagattt tgcttgttgt cgatgccatg
     7141 actggtcaag atgccgtcaa cgtagctgat agcttcaacc agcaattagg tattacaggg
     7201 gtcgtcatca ctaaacttga tggggatact cgaggcgggg cagcactatc gatccgctca
     7261 gtgacaggcg caccaatcaa attcatcggt tctggtgaaa aattgactga cttagaagta
     7321 ttccacccag atcgtatggc tagccgtatc cttggtatgg gggatatgct gacattgatt
     7381 gaaaaagcgc agcaagacta cgacgagaaa aaagcggaag aacttgcaca gaaaatgcgg
     7441 gaaaattcat tcgatttcaa tgactttatt gaacagcttg atcaagtgat gggtatgggc
     7501 ccactggaag atctaatcaa aatgatccca ggaatgaatc aagtccctgg aatcgaaaac
     7561 gtcaaggtag atccaaaaga tgttgaacgc aaaaaagcga tggtttattc tatgacgcca
     7621 gcagaaagag caaatcctga tctgttgaac ccaagccgtc gtcgccgaat tgctgccggt
     7681 tctggaaaca gtgttgttga agtgaatcga atgatcaaac aattcaagga atcaagaaaa
     7741 atgatgcagc aaatgtccaa aggggatatg aacatcccag gtatggacca aatgtttggt
     7801 tcaggcgtca aaggaaaact aggcaaaatg gcgatgaatc gaatgatcaa gaaaaataag
     7861 aagaaaaaga aaaaacggaa ataagatgaa aaaaacagct tcggtacaga ctgaaaatgt
     7921 ctactgccgg agcttttcta tcagcaattt ttattcttta ctaatacaat tactctaaac
     7981 gaagaggtgg cacagatgag atgtccgtgg agtgaacaaa cagaaagtat gcggcaatat
     8041 catgatactg tctggggtgt tcctgaatat gatgaccaaa agctgttccg aaaattgatg
     8101 cttgatatta atcaagccgg attgagctgg cagacgattt taaataaaag tgaagcattc
     8161 gatgaagcat atgatcgatt cgatatcaaa aaagtcgcaa attatggaga aacaaaaatc
     8221 agtgaactaa tggaaaacaa aggaattatt cgtaaccgaa gaaaaatcga agcagccgtc
     8281 caaaacgcca aagcagtctt gaaaattcaa gaagaaatcg gcagtttttc agagtatctt
     8341 tggtcgtttt ctaatggaaa aatcgttgat tcttcttatc attcacaaga agaagtccca
     8401 acaacaagtc ctctttcaga tgaattagca aaagatctaa agaaaagagg atttaaattt
     8461 atcggaagca caacaatcta tgctttttta gaagctgtcg ggatcatcaa tgatcacctt
     8521 gacagttgtt tccgcaaaga agaggtcgta cgtatcggca gaaagggcga ataaagcatg
     8581 cgaaaaatca tattttatgg tgctataagt ttagatggct atttggcaac gaaaacggat
     8641 gatcttcaat ggctttttga cactccaaca ggtaagcaaa ctacttatga agctttttat
     8701 gaaacaatcg atacgaccat catgggcaga aaaacttatc aggaagcaaa aaaatatttg
     8761 gagaccgaag caatttatcc agagaaaaag aatttcgttt tttccaccca cgcacaatta
     8821 caattgccag atgctgaggt cgtaaacgaa gatcctgttg cttttctaaa aaatctacaa
     8881 atgacagaag gaaaagatat ctgggttgtg ggtggcggtc gattgatcaa accgctattg
     8941 gaacatcaaa tgattgatga atggtatatc cagattaccc ctgtattatt aggggacggg
     9001 attcctttat tccaaaaagg aacatatgaa gcgcgttttg aaatgctaga cacgacacga
     9061 ttcggtgaat ttatcgaatt gcattatgta cgaaagtaga agtaaataaa ataaaaaagg
     9121 caactatatg cagaaatgtc ctgtacatag ttgccttttt tattaccgtt atgcttttta
     9181 ctctgttaat ttgttagaaa aatctagata cgcatctaaa atcgtttgga agaaacttaa
     9241 tgttccttca acgatattac cattttcatc aattagattc gcaacgtttc cgatatatgc
     9301 ttccggttgc tgcaaggttg gcacattcaa aaagacaagt gattggcgta gatggtggtt
     9361 tgcgccaaag ccgccgatcc cacctggtga tgcagtaacg actaaacctg gtttatgatc
     9421 ccaaacactg tgtccataag gtcttgagcc tacatctaaa gcatttttca atactgctgg
     9481 cacagaacgg ttgtattcag gagtaacgaa aacgacaccg tctagtcccc tcacttcctc
     9541 acgaaaacga gtccattcag caggagcctg ttctggagtt tcgagatcct cattgtaaaa
     9601 tggcagatca tcgattttta caaatacact ttcgtaccct tcaggaagca atttcgcaaa
     9661 agcttctgct acttttttgt tgtaagaatc ttttcttaaa ctaccaataa aaaatccaat
     9721 tttttttgtc atacgatttt tcctccttca aactatctat aataatctta caaaagataa
     9781 gtaactggag caaataaaaa cacttactga caaaagctac atagaagata gattacttat
     9841 ttttaacaaa aaagaagtct aaaatcttct attttctaaa aaaaatcgaa tttaatcaag
     9901 ctgtaaagaa aaaaagcttt acagggtaaa aaaaaactgt tacactacat cttgtgaata
     9961 aaacaatgga ggtggaaata aataatggca gttaaaatcc gtttaaaacg tatgggttct
    10021 aaaaagagtc cgttttaccg tatcgtcgta gctgattcac gttctcctcg tgatggacgt
    10081 ttcatcgaaa ctgtaggtac ttacaatcct ttgaaagacc ctgcagaagt agttttaaaa
    10141 gaagacttag ttcttgactg gttatcaaaa ggtgcacaac cttctgacac tgtacgtaac
    10201 atcctttcaa aagaaggcgt tatgcaaaaa caccacgaag ctaaattctc aaagaaataa
    10261 ggtgaccgga tatgaaagat ttaagcgact tagtcttaac aatcgttcgt ccgctagtca
    10321 cttatcctga tcaagtcaag ttagacgtcg tagagtcgaa agacttttac gagtacaatc
    10381 taacagtggc tcctgaagat attggccgta tcattggaaa acaagggcgt gttgctaaag
    10441 caatccgcac aatcgtttac ggtgttcgga tcaatgcacc gaaaaaagtg cgtttgaata
    10501 tcatcgatga taaggaaaag taaataaacg tgctgtactc gaattttcga gtgcagcttt
    10561 tttgtttttt ttattaattg aagaaatgat tatttggcaa aacaagcaag aaagcgtatc
    10621 cttataggag gagggagtga acagccaatg agaaagaaaa tgttatttat tccatttgct
    10681 ctattgttaa gcgcttgtag ttcggttact caagaatcag acaatacaca tctatcaagc
    10741 agtatatctg aaactgttac cagtccagaa aaaagtagta ccaaaacggc ttccaccact
    10801 gaacaaacaa tggctacctc aaataatgag aaaaagactg ctttagacca gttgaaagaa
    10861 caacagccaa atgttcccat gcctctagat gtaccagtct cttcaggcta tctcaatatc
    10921 gcagcgacac atacaaagca aggttattct attttatatt acagaacaga tcgtcccctc
    10981 gggttgaatg ctgacgaatt gaatcaagaa acgcctattg caacctatct atatcaatat
    11041 gggtttgctt ctagtcaaga aacaatccaa gttttgcagc catttgagat cgacacaaat
    11101 ggtcagcaag tggacttagg ttctcgaatc acagggtatc agcaaggtgc agcaggttca
    11161 agttttcttg aatggcaaga aggaaactgg cgtattcgaa tccgtgggaa taatatagaa
    11221 ggccaagatc ccttactatt agctaaagaa atcgtagctt atttggaaga aaacagtcta
    11281 ccagcacctg aacaatttgg taaaattact gttgatatgg gtgatacgac gaatcgagcc
    11341 gtagaagtaa gctggcaaga accgaaaaat gcctatacga tcactcatca agatcctatg
    11401 tctgcgttga agatggcagt ttctatgaag aggttgtgaa atcagcgcgc tgatctgagc
    11461 ccatcattta actccaagtc acaggaagta atcaaaaata gcacgccgca tttttgcata
    11521 attttggctt aataccttaa aagaaaacta gaattgcctc ttcacacatg ctaaaataaa
    11581 acagacaaaa aagaaatggg gatataagtt gactgaatac ttgaatgtag gaaaaatcgt
    11641 aaacacacaa ggtatcaaag gcgaagtccg tgttatctca acaacggatt tcccagaaga
    11701 acgttacaaa aaaggaacgg tgctaacact attccaagac ggaaaagcac ctgtggaatt
    11761 aacagtcaaa agtcatcgca aacataaaaa ttttgactta ttgagttttg aaggacatcc
    11821 ttctatcaat gatgtggaaa aatatcggga tggcatatta aaagtgtcaa aagacaactc
    11881 tgtagatcta gctgaaaatg aattctatta ccatgagatc atcggtctgc atgtattaga
    11941 agatggaact gagttaggta aagtaaaaga aatcttgtca ccaggtgcca atgatgtttg
    12001 ggtcgttcaa cgtccgaaaa aagctgatat attgcttcca tatatcgctt ctgtcgtcaa
    12061 agaagtcgat ttagaagctg gagtggtccg tgtagaaatt ccagaaggac tgattgacga
    12121 tgaagattga tgtattgact ttatttccaa gaatgtttga aggtcctatg ggggaatcca
    12181 tcatcggtaa ggctgtagat aaaggattat tagatatcaa tatctctaac ttccgcgatt
    12241 attcagataa caaacatcag acagtagatg attatccata cggcggtgga gcaggtatgc
    12301 tgctcaaagt ccagcccatc tatgataaca tccaagccat tgaaaaagct gcaccggatg
    12361 tcaaaaaaag agtaattctt cttgacccag ctggtaaacg attcgatcag aaaatggcag
    12421 aagaattttc acaagaagaa cacttagtat tcatttgtgg tcattatgag ggatatgatg
    12481 aacgtatccg cacattagtg acagatgaag tttccttagg agattatgtc cttactgggg
    12541 gagaattagg tgcgatggtc atgatcgatg caacagttcg gcttttgcca gatgtgttag
    12601 ggaatcagac ttcagctcaa acggattctt attcaaccgg tttattggaa catccccaat
    12661 atactcgccc ggcagaatat aaagggatga aagtaccaga agtattgaca aatggaaatc
    12721 ataaattgat tgaagaatgg caattaaaag aatcactaag aaggacctat caacgtcggc
    12781 ctgatctttt ggaaacactt gaatggacac ctcaaatgac gaaattttta gaagaaatca
    12841 agaaagaaga acaagaaaaa acagcagatg aagccaagtg actttttcta aaatcttctt
    12901 tacagtaggt tgataatatg ttagtatttt atggtgagtg caaaagcact taactattac
    12961 gatattccgc tgtgagaaaa gctcataaga atatttggaa taaggagaag aaaaccatga
    13021 atccattaat cgaagaatta acaaaagaac aattacgttc tgacatccca gcgttccgcc
    13081 ctggtgacac tgtgcgtgtt cacgcaaaag ttgttgaagg tactcgtgaa cgtatccagt
    13141 tatttgaagg tgttgttatc aaacgccgcg gtgctggaat cagcgaaact tatacagtac
    13201 gtaaagtttc taacggagtg ggcgttgaac gtacattccc attgcataca ccacgtgttg
    13261 caaaaatcga agtagttcgc tacggtaaag tacgtcgtgc aaaattgtac tacctacgtg
    13321 cattacacgg aaaagctgct cgtatcaaag aaatcagaag ataaacacgg gaaacttcct
    13381 tggaaatctg aaatctgtga aaagtccttg tatatcaagg gctttttctt ttttcactat
    13441 cacagtttta agcctcaatt ggctattttg gatcattttt cattcatatt ccattaaatt
    13501 gccctagatt gtccgatttt gactatcagt gtcgtcaaaa tagggcaaaa tttcgtagtc
    13561 aatttgacga caaatttaat acgaaaaata ccaaaacatt taccgaattt agcaattttt
    13621 gaacccgagt tgctaaaaag gttgaatgct ttcgaaaaaa gcaaaacata taccattgtg
    13681 ctaaaaagtt tatgtgatag gtttctattt aaataatttt aggagttttt tgtaatggga
    13741 aaagataagc gtattgtttg gaaggatcaa agcgatttaa aaattatatt aacaattagt
    13801 cagtttattg aaacttacga aataaaatct agtcgagaat atcagaaaca gttatcgaaa
    13861 aatccacatt cagctcccag catgtggttt attattaata aatatggctc gtggaataat
    13921 ttgctgaata gtattggaat tgataatagc gaatctaaaa aatgggctag aatgaagacc
    13981 gatgaattaa taaaagtggc acaaatattt attgattcag aaaaaataaa gtcacagcgc
    14041 gtttatgaaa aaaaatcttc tggtaaagat gttccgtgtt taagtacttt aaagaataga
    14101 ttgggcgata ttcgttttct ttttaaaaag aaagtgagta agagattaac taattttgag
    14161 atacttttag agcttaagaa tgaaataatt agactcaaca tggaagatga tttatctatg
    14221 acaagatttc aaaactattc aaaagcaaaa catcttccat ctgtgtatac aattatgaga
    14281 cggactaata agacatagga agaattaatg agggaaattg gatatgatta tagagaaatt
    14341 aaaatgaaaa aacagagaaa taatttgtgc tgtagatcaa aaagtaaatc cggctaagag
    14401 taagataatg taagttgtat agtaatacca actgaataat tgagaatttg gtgggatatg
    14461 tagcttcttt ttcaatagac acatgcaaaa aagaaattaa aataacaagt aaaaaacttc
    14521 tgacagaaca attcgttcac agaagttttt ttgttgtttg gtgaatgaca aaggaggaaa
    14581 tatagatggc agaatcaaat gtgtttgttc tatatacgtt agcggatcta aaaacaggga
    14641 catttgtgaa ttattacaag atattcaatg agatcgaacg ctattattat ttgcctattg
    14701 aagctgggat tttatatgga ttgatggtag atcgtctgat gttcaatgaa caggatgaac
    14761 gtaccttgtt agacaaagag ggaaattcct acattttgat gacggtcaaa gagatgcagt
    14821 tttatatcaa agtacgttcc gaacaaacca ttcgaaagat cattgaacaa atggagaaag
    14881 aagagttgtt cgattctttg aatggtacaa acaccaaggt aaatttcgtg aatacctggt
    14941 gatttctgaa tcacacactc gaaaaaagcg atacaagacg caccgatttt acttagacac
    15001 ctacgatttt gcggagattt atccacaact tgcgacgatc tatgatttga acgggctttt
    15061 gattgctcag cgtagacatt cataccagct tttttattgg gtatgtcgta ggacatttta
    15121 ccaaacagac acacaagaat atctgacaga gtatcaactg aggaggcgac gaaaaaggaa
    15181 tggacagaaa tcaagcattt cctgaaagat ctggcccaca ggtaaaggat caagggattt
    15241 ttcggatgac ggatatccaa cgtctcggtg cagactgtca gtgttttccg ggtttcgaaa
    15301 ccgtttctct ttcaggagga aagacccggt tcgttcggcg attacgaacg tttcgtgtcg
    15361 aagacagaga cgttgtggaa tttgaggaat accatgaagg aaaggagcgt aaaatctatg
    15421 aaaaacaaac cttgccctta aaagcgatct atctagagat caaacgcgta acggatgtgt
    15481 caggaaaaac gattgcacgc cgtttgaaac ctgaacagac gttacgttgg ctgttttcaa
    15541 caggacggca gagtaaaggc caaaaattga atacaaaaac acttgatgtt ctctttttcg
    15601 atgtacctta acatctgatc gaaagcaatt ggacgtttgg aaaatctatg aaggacttag
    15661 tgactatcgt agcacttagg gaatctgcgg ctaaagttag ggcatacgtg ctagaccttt
    15721 tttgatatca gcgttggttc tcttttacgc atctacaaca acgaattggt agcatgagtc
    15781 catgcggtag aaactgggaa tggaaatgaa cggaaacgat gaggattttc ggtaaaccat
    15841 agagggcaca agaaagcaga ttgtcggaac gatcagcgat agcgccttat ctgatcagaa
    15901 ccgctactaa ggggataagg ttacgtacat ttttgaaata gaaaatcaga tttccagtaa
    15961 tgtagagatc acttagaaac aagtttttga aaagactttt tctgagcgta tgcgggagta
    16021 catttaggca cttgccaaga gcgcaaattc attgtaggag catttaccgt tggctactag
    16081 ggtttctgga tccggacgct ataatccagt cgaatgcttg ttagggtaga tcgaagaaga
    16141 gaacagagaa aagcaaagaa ggaattaatt gaaaaggaga gagttttttg aagcaagaaa
    16201 aaatacaagc gagaagaaga aacgacgagc agaaggggat ttcaccaaag aaacaactct
    16261 taaaagagcc caaagcaatg ggggcgctcg gacaaaagaa agatcaaaaa atcaaagttg
    16321 cttttttaga cgggaaagtg atggtaggaa cattggttca actgatgaaa tatgaattgg
    16381 tgctggaagt agaaggaaaa gaacgcccaa tcgccgttta aacatgccgt gaagtatctt
    16441 tactagccaa aacaaggggg gagagaaaca tggcacggtc tgaaagagaa cgaacgccaa
    16501 aatattgttt cacaggcgaa accaaatcgt atcaacaccg gacactatat cggattcaag
    16561 cgtttggaac cgtgaaagct ggcgaaatta gtgatagcct gcataagaag cattttcaac
    16621 ggaagttgta gttttggaca gaaagagagc aattatttta ctgataaaca tgtcttcaga
    16681 ttatcttttt cagatctgtt gacccgtgcg agtgtcgggc tcctcccatc agtgtttttc
    16741 acattctttt ttcactgggt tatcggttta gacacctccc gacattcgta ggagtcagca
    16801 gattaaaaaa gatagttgaa gggatttaag agttccaaaa ataaatcaat aaaccattct
    16861 ttaaagtttt cccagaaaga ttcttttttc acagcaaagc gataaacact gatgaaaaat
    16921 gcaacaatca ggcaaagagt aacgtacaag aatcaatagg atttctttta ttttttccat
    16981 gacaaagact cctttatcta gtgatcaagt aaagtatatc agggaaaggg aatgatcgca
    17041 tgacattctt gtaagaagaa agattcgttg aataagtgtt gatcgagagg aaaaatatac
    17101 aacaagacaa actataacga atgtcaatgt taaataattg gatttaataa caatattttg
    17161 ggcaagccaa ataaacgtct tgtttttgat ttttttgaca aaacgttcaa aggatataga
    17221 gaggggggat ggagattcaa tttaatttta cttcttcaaa gaatatttga tgctttagaa
    17281 gatgaaaaat gactcggaca agtttttttg caacatgagt gatagccaca ctatagtgtt
    17341 ttccttcagc ccttttttta tccagatacg ctttgaatgt cggtgagaat cgagcagcta
    17401 atttggcagc ttgtattaaa gcccagcgca aagaagtaga gccacgtttc accatatggc
    17461 cttcacgatt atactgacct gactgaaata cagagggctc taaacaagcg aatgcaagga
    17521 gttgtccaga ggatttaaaa ttatgaatat ttcttatctc agataaaatg acactaccta
    17581 atcgattttt tacgcctgtt atggaagtaa ttggtgaatt tattttcgcc attaacagat
    17641 cggtttctgt gtcgatcgta gctatttggg tcgtatatag ctcaatggtt tcaattattt
    17701 gaagcaactc taattcgaga ctagtagtcg aaaagccaat tgtttgtttt gaagcttctt
    17761 ttatcctatc aatcgtttcg gaagtaagtt gtcgtccacg atgatttgtc aggactttaa
    17821 ataagttttt atttcgacat ctactaatct ttttaggtga aggataagct ttaaacatat
    17881 tgtaaatgta ttgactatga atatttgctt tacctacaat tggcgctagc tcaggaaaca
    17941 taatatctag caatcgaacg aattgtgttt tcagttgagc tcgtttctct gtagtgcgag
    18001 aacgatgtct agtcaaaaac tttagttctt gataaaaagg atcaacttca tgaatggatg
    18061 gatccaagtc attcagtaat tttttagcta ttgttagtgc gtcacttttg tcagtttttg
    18121 tttttctgag actatcaact ttcgcaaatt ccctaataat aattggatta tagctaaatg
    18181 ttggataacc acatgaccgc aagaaacgta tgagattgaa gcaatagtgt cctgtatctt
    18241 ctagagcaat ttggatagag gcttcgttgg tggattttcg tagggattca agagtaattt
    18301 gtaattcagt gaacccttca cgatcatttt ttacttgttt gtgattcaaa tggatagttc
    18361 cgaaggaatc aatcactgag aaatcgtgtt tatttttggt tacgtcaata ccaacaaaaa
    18421 gtgtcataaa taaatcagct cctaaatttt tattgtttat acactgctag tccacgtact
    18481 ctttgtatcg tatccttaca cgaaattaaa tgtcgatgca ttatcaaact aattaccgat
    18541 taaacaaaga gctgtggtta gatcctagtt ttactagtta ttccgaaaga aatactattg
    18601 gaggagatac taatccacag cgcatatatt gagtataaaa taacccaata aaaagcggga
    18661 gctacccgct attaataata taagggggag taaatttgaa atttaaaaat gaacaagcgg
    18721 aaaaagaatg gcaagaattt cggaacaata accaagatcc ttacggctca ggtttggtta
    18781 gatttgcaga acgctgggct gaattaatgg aaaaggaact tgaagcagga aaacccttga
    18841 tcgatattgc aggaataaca gaaaatcaag cagatataga aggaatcact ggctttgtgt
    18901 atggtgtggc agtcaatgtc ttatcgcatg cttggaagta tggagaggat ctaagagatt
    18961 ggcataatcg aaaatatagc tatgatggta gcggagttgt gaatccagta atcatgtctg
    19021 caggcaaaaa agaatagaag aaggaaattc gatggatttg aaaccattgg aaaaagagca
    19081 ggaggaaaaa atgaatgaag aaaattatat ctgtagtttt ggtatcaatg ggactcttcg
    19141 tatttactgg ttgtacaaca gatgcggata ttgcatctaa taatttgagt gaagatgccg
    19201 ataattttaa agtattacgg aaaattacat ttatcaacac cgtaacggat gaagtgctgt
    19261 ataccgttga aggaaacttt tctatcgcag cagatacaga ggataaccag ctggagatca
    19321 ctgcaaaaac tggcgaggat caatttcaaa agtatttttt aggcttatca ccaacgacgg
    19381 tttatatcgt ggaacaattg gaatggcaag aaacaaatca atatcagttc aaaatcacca
    19441 ttaagccaag tgcagtatta ccagatgttg atattcgtta ggaggaggaa aaatgacaga
    19501 agttgtgtat gtaatcatgc tattttgtag ctgtttgttt ttctggggat tgtttcattt
    19561 ttttgggtgt tgcatagatt ggttggttga tcagatttgt acgttgctag aaaaccaaca
    19621 gaaacgattg gattcgaata aaaaatgact gtcgtaaaca gataaccgac agtcattgag
    19681 ctagatcatt gaaattagtg ggtatgtgca aacatgatac tgaaaggaaa gagctagctc
    19741 atagttagta tataacagaa agttaagaga aaaaagagga aatgaatggt tggttgactg
    19801 gtgcgagtat ttgaaattcc ttgtatttag accattgaag ccgaaaaaaa ttccaacaaa
    19861 catgattttc aacatggaat actcgtactg gtcagctaat caaaaaagaa aggggcacga
    19921 aaagaagatg aataaagaag tgaatggttg tgtggtgttt gaaaaacaag ggaatgattt
    19981 ttcggtgaag gcaggaaatt taagtgcggc tgatgcctta acgctaacgc tgtttagtat
    20041 tgtttctctg gcaaaggctt ctgggatttc gctggaatgt attcacaaaa cattagatca
    20101 gattgatcag cacttagtgt tagaggagtg tgggcagaag aatgagtgag ggaaatgcga
    20161 cagttaaaaa gctggcgaga aaagaattat tcgaattaac agatgaagaa cgacacccga
    20221 attgggcgga tgatccgcaa gcaatcaaac gacgtgatca gttacttgtg attttaggca
    20281 tgcccatcga tcctgttcgt aaagaaggag aaacgaaaga agcgtttcaa aaacgatcca
    20341 atcagtattt ctttgattta cgtccaggat tagaagaaaa ggttgtttct cgattacttg
    20401 caggagaaaa aatgaaacgt ctatctgaag agtatcagct tcctcgatcg aaattgacgt
    20461 atctacgaga aaagtatcac ttgttggaaa agtagaatag attcattttt attgagctac
    20521 ttggtacatg aaatagtggt tgtctttttg agctaaccca atttgttggg caatgataag
    20581 attcttcgat gcatatttta ctgtaggaag tgtgatttca ttttggtctt gattggtaat
    20641 ctcgatagga aagcttaatt cttttccgtt ggctgtggtg agaatggctt tggttttccc
    20701 tttaaaaatg atagtttcaa tttgccaaat accgagatct atcaaattgt ccccttcttt
    20761 ttcttggagt tgggtaaagc caaaaagttc tgtttttttc tcattatcag ttgaaaacgt
    20821 tgactctgtc actgttaggg tttctttggg cttttctttt aagtgatcga aagctagaaa
    20881 agctaaaagg cctactagta tcaagaggcc accgaataag tatttggata gttttgtcac
    20941 gtcaattgat cctttctccg aagtatttgt gtagaagcta ttgataagca gattagagaa
    21001 gcgttgtatt gattaagcaa tttgaaaaac gaaccttcta cagagtgtga catctataat
    21061 ggatatggca atagaaggtt cgttaaaagg ctaaaagaga gagttcatga acttgacgta
    21121 ttccaacaac ttttaagtag taaaaatgtg tattttttag cctttcttat agaataataa
    21181 actatttcca gttgtcaata gtaggaatta ggagagtgtg gcagcaagga attagaaggg
    21241 agtgttttta tatataataa aaagaaatgt aaggataggt gataaatgga ttgaatctga
    21301 aaaacgtttt tcataaaata ggagtaataa agtaataaga ggaaatatca ttttctcatt
    21361 gtaaaaaaat cagctagaga aatagtgaag aatttctatt cctagtatta gaagtttttt
    21421 ctaaactatt agtttctgtt tttcaaaaca gagaaaaagc aatgatcggt ataggtatct
    21481 tagatataga aacattgttt tttcgataaa gaagcagaaa cgacttatcg ttgtttctgc
    21541 tttttcgctt tgagctgttt ggcgtaggct tcaacatcac tttttaaata caatcgtact
    21601 agtgaagttg caggatctcc gtcaaattca agaaaaggag tgataacttt tcggttaatt
    21661 gcttgagaaa ttccagcata actttgaccg gtaattttag cagcaccttt tctagtgaca
    21721 agattttctt gagcccagat ttgaaatgat ttgatagatt ctgtatccaa tatgttcacc
    21781 tcttttattt atgatcgtaa gcttattata ctagaaagta aaattttttc aaaaaactgt
    21841 ttacaaaaga ctataaattt aatgtcataa acttatagtc atagacaaac aataaaaatt
    21901 aggaaagaag gagatattat gaaactaaat gtattgagag tgttagcagt aggaatagta
    21961 gttgggggaa ttgctttggc aatgaataca gaaaaagcag aagcagctga atggtctcct
    22021 cgatctgtcg aggcaatcaa agcagacatt gcgaaagcta aagataatca atatacgatt
    22081 gtctgggggg atacattgag tggaatcagt caggcgacga atttgacggt acaaaaactc
    22141 gcagatctga ataagattgc gaatgtcgat ttgatctacg caggcaatac gctagttttt
    22201 gacgggaatg tcgtgacagc taagaacgat aagggagaaa cggttgcgca atcagtggta
    22261 accccacaag ataagaatga tgcgaataaa ccagtaggta aacaagcgcc tgcaaaacaa
    22321 gaagaaaaag tactaggaac aaccgcgtct tctcaatcac aaaacaagca aggaacacct
    22381 gcgacaagtg gaaaagctga tactaataaa caagggacaa gtgctgcagt aggagaatat
    22441 ggtcaacttg aaggatttta ctcaaaagaa tatgtagaaa atggttggaa atatgtagaa
    22501 gccggaccat ttaagacagg aaaagacttt ctaaattggt ttgacgcaac atatcctgga
    22561 aaaggcggcc cagggtactt tattttatcc ggtgaacaag atttcacaat tggagataat
    22621 gttaatttag ttgccaaaaa agcgattgaa tatgtatgtg attcaggtaa tacaacaact
    22681 gaaaatgcag aaacacctgc tcaaccaagt gagccagtta cgccaagtca accaacagat
    22741 aatcaatcaa cacaaccaag caaaccagcg gaaaaaccaa gcacatctac tcaaccttcg
    22801 acaccgacac aaccaagtac aggtggagaa caaggtggat cgacaacacc aaccacacca
    22861 gacactaccc ctattaatat gggaaatagc gggaaagtgt ttgattcaga agaagcggct
    22921 tctgaatatg gatatgcaga gatcgcaaat gaaaagagtc catggtacgg ctatcgattc
    22981 ggcgcaagtc aattagttaa taaagattat gaattattag gaacttggac agttgacttt
    23041 acggaaaata agccttttga ttggaatgaa tagtttaaaa agatatcagt aaatatgctg
    23101 atatcttttt tgtttggagg aaaacatgat gagaaaattt ccacgggcaa gatccccaga
    23161 tcaaccaaaa taaaaccaag catttacgtc gttatcgttt ttggtaacgg cgttttatta
    23221 tagaaaaatt taagaaagga agcgattctt gtgaggacaa gaacgaaaaa gaaatggtta
    23281 aaagcagctg cgcttctggc attttctcag ttctgctttt tgaccgggac gtttcaagca
    23341 caagcagaag aaacgacacc cagtcaacca gcggtagaaa cagctgtcgc agctcccgtt
    23401 gaagccgttg aagttcaacc ggtagaagaa gccgttccag cggtagaaac accagctgag
    23461 cctatgccag agaccgatgc accggcaacc aactcaaaag cagcccttaa tcaaacgatc
    23521 ggggaagcac aaaacccagg agatagtcca gcagtcacac aaccggtaga accagaagca
    23581 ccaaaaacaa acagtttacc ggaatcatca gaagaaagta cgtcgaatac aacatcttct
    23641 tctgaaccaa cagagtcggc agaaatacca gaggcactga caccagaagg taccccaaca
    23701 cctgagatac ctgtctcacc agaatcttct gtagaaaaaa caacggatac acccgctact
    23761 gatatagaaa aaccagcgga acagccagta gacactaagg acacggaaga aaagacagaa
    23821 gcatctgagg tagtttctga cacgaaagaa acgacgttgt cggataaaga agtagaagaa
    23881 atgttcgttg tcacgcaacc tcgggcagct cgtattcgtc gtgccgccgt ttctacacgt
    23941 gccgcgaatg ataatattgt tcgtgaaaat ttagtgggac aaggctacaa ctgggcagtt
    24001 gttgatgatc cagggcgtaa aggttttaca ttcaatgcca ttccgatgtt tacggtcaat
    24061 gggcaagccg cattttgtat tcaaccaggg gtagatttta cagccaatgg acaagttcat
    24121 tctactgaag cattggatac gtatttgaaa gatgccaata aacgaagtca gatttctctt
    24181 atttcttatt ttgggtatgt taataatccg gataaatcga aggaacaata ttttacgaca
    24241 caattaatga ttggagaaat attaggaaga aaagttgttc ttacgaatgg attgaactac
    24301 gaagcgcgta aagcggcgat tcagaaatta gttgatcaat ttcatactag agcttctttt
    24361 aacaatcaaa caaagacagt aaaagttggc gaaacgatca aagtgactga tacaactggt
    24421 tttttatctc gagtgaaaga ggttaaagcg cctaaaggga ttaccgcaaa gattcaaggc
    24481 aatcaattgg taatcactgt gacaaaagat gcgcctgaga aagcacaagt aagattagcg
    24541 caaattaaac ttgctagtgc tcctatggta tacaagaaac caggatcaca aacaattggg
    24601 gtactgaatc caacagaacc tggagattca attttaaacc taaatgtcct aaaacaaggc
    24661 aatcttgaaa tcttgaaaat tgatgcagat acaaaagaac ctttagccaa cgctgaaatc
    24721 aaagttacaa ttgctggcaa agatcaaacc gtgaaaacca atgataaagg gattgcgaca
    24781 atcaaaaatt tgacgcacgg cacaaaagga acagtcgttg agatcaaagc cccaagtggt
    24841 tatgttttaa acaaaacaag caaagatttt acgatcgaag cgaataaaac agtgagagtg
    24901 acccttgaaa acaaagaaca aaaagggatt gcgcgcattt tcaaagaaga tgccgaaacc
    24961 ggaaacaacg cacaaggtgc tgcgacctta gaagacgcag tctatggact ttatcaagcg
    25021 gatggtaaaa aaatcaaatc tgttacgttg aaaaatatca atggtcgtgt tcaagcggaa
    25081 attgcagatt tgaaattggg ggattattac tgggtcgaag aacaagcacc agttggttat
    25141 aatctcaaca cgaataagat ccatttttca ttagtttatg gtgatcaaaa tcaagagact
    25201 gtcacaacag aagtaatcgc aaaagatcaa gtgatcaaag gaacgattga aggggtcaaa
    25261 gtcggcaata aagattttgt ggacgcaaat gatcctgaca ataaaccgaa attagaaggt
    25321 attaaaattt ctttgacctc gaaaacaacg gggaaagtcg ttaaagcggt attgactgac
    25381 aaagatggtt actttacctt cggtaaaaat gcggttgttg ttgacaccta tattgtttct
    25441 gaaaccaaag gcaaggaagg ctacaaattg tttgaaccgt tcgaagtaac gatttctgaa
    25501 caaggtcaaa ccttccgtta tgtgttagaa gaccaaatca ttgaacaaca gttgaaaatt
    25561 gtcaaagtcg atcaagaaac aggcaaagtg attccacttg ctaatacaga gttcaagata
    25621 tttgacacct tagcgaatga gtatgttatt ttttcgattc caaatgatac cgaagtaacg
    25681 gatattttcc ggacaaatga caaaggatac cttgttacga acggtacgtt tacctatgga
    25741 aacaatcgct accgtatcga agaaatcaac gcaccgagaa attatgtgtt gaacaaaaca
    25801 ccattggtgt tctcgattac cgatgataca gaaccgatca aggtgatcaa ttttgcgaat
    25861 cgtcaagcac gaaaaaacgt gaaattgttc aagtatgaag aatggaacaa gaagaaaaca
    25921 ccgcttgccg gtgtgatgtt tacgctgtat aacggcgaag gaaaagcctt aggggaatac
    25981 accactgata ccaacggtga actcctagta aaaggcttag tcgctggatc ttactacttt
    26041 gtcgaaaaag caccattaga aagcttccaa ccaaatacag acaaacaagc attcactatc
    26101 acattcaaac aaaatgcgcc agatgatggc gaaaccttgt tggtgtctgt acgcaactat
    26161 ttgattccac caacacttga aacacaagcg tcgaataaag aagacggaga aaaagtggtg
    26221 gatccgttag aaacgatcgt cattaaagac agtgtttctt ataccaactt gtttgtcggt
    26281 aaagaataca ccatttcagg tgtcttgatg aacaaagcca caaatgaacc gatcttgcaa
    26341 gatggcaaag aaattagaaa aacgttgacc tttattgcag acaaagccaa tggcacaaaa
    26401 gacctggaat ttgtggtcaa tgccagcgtc ttgaaaggtc aaacgatcgt tgtctttgaa
    26461 aaacttttcc gtgatggtat tgaagtcgca gcacacgaag atattaccga tgaaaatcaa
    26521 agtgtccggg taaacaatcc aacggttcat accacagctg tctttgaaga cgggttgacg
    26581 gtcgcagacc caacggggct atttaccttg aatgacactg ttgaaattac cgagatcctt
    26641 ccaggaaaag cttacttggt caaaggaatc ttaatggaca aagcaaccgg tgaaccgttg
    26701 cttttgtatg gaaatacggt agaaactagt gttggcttta ttgccaaaga agaaaatgaa
    26761 acggtgatcg taccatttac ttttgattta acggacttaa atggtcgtga aatcgtcgta
    26821 tttgaaagtc ttttcagaaa tgacgaagag ctggcaaacc acaaagacat caatgataaa
    26881 ggacaaaccg tacgtatcac aaatccagca atcggcacca aagcaacaga caaagaaaca
    26941 ggattacaag tgattgatcc attggctgtt ataacgatta ccgatgcagt tgcctatcat
    27001 gatctgattg taggaaagac gtatacagtc aatggaatct tgatggataa agcaaccgaa
    27061 caaccagtct tagtgggcga caaagaaatc acaagtacct taacctttgt ggcagaaaca
    27121 acggacggta cggtgaatct agactttgtg ttggacgcac gtgcattacg tggaaaagaa
    27181 gtcgtggtct ttgaagatct cttcagagac ggtgtattat tagcctctca tgcggatatt
    27241 acggatgaag gacaaacggt acgggtaacg aatccaggtg ttcgcaccac cgctacacac
    27301 aaaacaactg gcttaaaaga agtgaatcca ttaacgaaag tcaccattac agacgcagtg
    27361 aaataccatg atttaatcat tggtcgcaag tatacagtaa acggtattct aattgataaa
    27421 tcaacgaaaa aaccagtgtt agtggacgac aaagaggtca caagtacgtt aacctttgtg
    27481 gcagaaagta ccgacggtac agtcaactta gactttatcc tagacgcacg tgcgttacgt
    27541 ggaaaagaag tggttgtctt tgaaaatctc ttcagagaag gcgtactatt agcctctcac
    27601 gcagatatta ctgataaaga tcaaaccatc aaaattgtca atccaaccat tacgactaag
    27661 gccaccaata aaaatggtgg aaaagagatc ttcgctttgc caaatcaaac cgtgttggaa
    27721 tgggtcaaag taaacggttt agcaatcggt cagttgtaca agttgatagt tcaaggctat
    27781 ttaacaccag acggcacgcc atttgaaggc acacatgccg aaaaaatctt tacggcagac
    27841 aaagaaacca cggaattctt gtttgaattc ttagttgacg gtcgcaatct tgctggaaaa
    27901 gggttgagtt ttgcggaatg gcttcaagcg aaaacagaag atgaaaaaga aacgtttgaa
    27961 gaagtcgcac gtcacaacgt ggatctaacg aataaagatc aaacggtaca aattgtggaa
    28021 gcaccaaaac caccacaacc agaacaacca gtgaaagtag cttctgtagt gcctacattg
    28081 ccaaaaacag gatttcaaga taatggtgta tgggtcgtga ttggattaat ctttgtcctt
    28141 gcggcaggat tgattggtca tgacctatac aaggtcaaag actaaaaagg atgaacaagc
    28201 ggttagacaa ctagccgctt gtttttagac aatttggaat gatcaagaaa gagtaagaga
    28261 gttggttatt agactactca caacattcta aaaaccggaa gggggaaagc tccttatcgc
    28321 ttactcttca gtaaaaggca gcacaactca tgggtgtact gccttttact tataagtgga
    28381 ggtgaattgg gatgttttct tgtagtgatt gttatacggc agaggaatta cttgagggga
    28441 ttcgaatgta tcaagaagaa catgagtggg cagagtcgct cacgttgtca tgggagtatg
    28501 aaccaattga tccttcagtg attcaagcca tcgaagaagc gggctatttt tgtgaagcac
    28561 attttagtgc acttcataac atttcttatt acaagtgtta ctttgttcct gtaccagaaa
    28621 aacagcgagt gtgtaaccaa caacgaatca aggaacaaga agaacagcgc aagaaggaac
    28681 gacgatggga tattctagaa acatctttga cgatctttgt ccttcttttt tttattgtca
    28741 tgggttatgt tttatgtgtt tagaacaacg agatgcgtgg aaatacgctg tattttgcga
    28801 tttgagacat tttagtcgat cgattaataa aaccagtcga aaatatttta aaaccctcgg
    28861 agaaagcgat tctcgaggct ttttttgatt ggaggaataa tatgagactg tatattgttc
    28921 aaatattcta taataatgtt ttataacttg tcgaagatca ttttaggatt tggatgaaat
    28981 taaaaaaagg aggaaattca gtgagtgaaa tgtttcaaag tgatcgtatt ttacatttgt
    29041 cacttggctt agatgttttg ctaaattata acaaagaagg aaccaaaatt aagacggttc
    29101 aattatcgaa aagtcgtctt ggcgagctga ttcgtaatca gactgtaact cgtaccagta
    29161 ttatgaaaaa tcaaaaaata ggactgatca tcggatgcaa tacgaatatt gtgattaatg
    29221 tggaagactt gattgggttt caagataaaa caccctttat tctcaatatt gatagtgatc
    29281 aaacagaaca aatgtatgaa ggaaaagata tttttcaaat gtcacacttt gagttcattg
    29341 tgtcaaatga acaagatctg cgaccttcac aaattaagca actttattat ataaacagaa
    29401 aatttcagga ctatcttcaa gaacaagagg ctgctaaaaa agcaaaaaaa cgaaaagcag
    29461 cgattcgaag aagtgtgatc gcaaatcaaa aataacatga ttgggaggat tatatgagtg
    29521 agggaaaaga gactgagtct cgcataacaa caaatacaaa gaagaaaata gccattgcag
    29581 ttgctttagg ggtcgtttta tttagcggtg gcttaggcat tcacctctta cattcgcctg
    29641 aaaagaacga gtctaaagaa atagaatata ctactataag ttcagaaaaa agtttagaaa
    29701 atccatcgat aaggaatgct gacaaggcta cgaagcgatc aaaaacaaaa atagttgaaa
    29761 aaaatcaagt agataggaaa aaagaaagta agagtaagga aaacaattcg caatcgacac
    29821 tgtttgactc gattttttca cgtatagatg aatcaaatga ggaacctgat caaacgaaag
    29881 aagcattgac acaaacacag ccattactta ctttacccga attaaaagaa atcgctaaag
    29941 taacagaaga aaaagagaaa caaaaagagt tactaaaaag caaacagaag gagaatgctg
    30001 aattggtttt ggtaggaaaa gagggggaag aaacagagaa agatgaggga gtagagccta
    30061 cacctctatc tacaccaaca cctattcttg atcctagtct ggaaccagag ccaacgccta
    30121 tcttgccagt tgaatcaaaa ccaaaaattg ttgtgagcca atcctatttc gaattgaacg
    30181 aaggtgattc gtttgatatc agcaactatt actctgtctt tgattctatg gatcaatcac
    30241 caagtgtgtg gattgagaat aacacgttaa gtgttggaac caacaccatg actattcatg
    30301 cggtaaataa atttggttat tcagatacag aaacaattac cgtacaagta aatggcagac
    30361 ctaaaattca agctatttct agcaatgtag aggtttcgat tcatgaaacc attgatctgg
    30421 cgacctattt ttcagccaca gatccagaag atgggaattt acaagagaag attgaattcc
    30481 tatcaaccgt caatactgga atcgaaggag aatacttggt aacggcaacg gtcaaagata
    30541 gtagaggagt cgaagcagaa cctacaacca ttcgattttt tgtcaaaaat gaagcgcctg
    30601 tcattcaagg tgagtcttcc tatacggtat caatcgacga atcattctca attctaaacc
    30661 tgttatctgt tacagataaa gaagatgaca gagatggtta tccgattaac ctgacagaag
    30721 aaaatatttt agagaacgat ttagatttaa cgaaagaagg tatttacacg atcaaaatcg
    30781 gaaatgtagc tgatcgtgat ggcaaaaagg cagaagataa ggggatcacg gtgaatgttg
    30841 ttaatgaggc ccctgtcgtt atggcaagtg accaaacgct taaaattggt gattcatttg
    30901 acccacaagt ctttttaaat agtatttcag cttctgataa ggaagacacg aaaaatcagt
    30961 tgacaccaac tatatcggtc gatgaggaaa ccttgtattc aattcgtaca gatatatcag
    31021 gtgtatataa aatcgcagta actgctatag acagtcaagg aaaagcaaca accgtttttg
    31081 cgactattac tgttgtagaa gatgattctg ccacaaccga atccccagaa accaaagaga
    31141 atgagctgga tatccctagt gtagaagctc aaagtgatca aatagctcct gaagaacccc
    31201 ttactgagaa aaaagagcat tctccgttat tgcctactga aaatcacata gattctgtgg
    31261 aagaagcgcc tgaagaatca gaggaaacgt cactagaaga aaagcaaggg caaggggata
    31321 gtgctggagg ttctgaactt gagagtggtg atcaagtaaa tgtacagaat ccaagcgtgt
    31381 gaatttctca tagataaatg gtgagaggat ttgatggcga gaaggttcgc tatcattctt
    31441 gtagttattc tctatcgatg ggaaaggaga gatagtcttg aatgaaaaag aacttgcgat
    31501 aaaaaggctg ctcatttcaa ttttagaagc aatttttgaa ccttcagatg tgtatcaagg
    31561 ctttattgtt agggtgattc caaaagagct aaaaagagaa ggacagtaca agcattttga
    31621 acgaacgatt caattgaata atctgacacg ctcaccgctt gaactttgct tgacttgttt
    31681 acggtgtctt gccatgcatg tcgatacagt gaatagcgaa ttcttttatg aatcagaagg
    31741 tatgtacccg attttacaga agctgctaga agcctcatta gaattgggga ttttttctat
    31801 tcgagaaatc gacgaactag aacaaagagg aaaaatcgat gatatagtag caaattgcgg
    31861 aaattctgca gtatggacat tgaagtatcc tctattgccg gttgcgtatg acgtctatgt
    31921 ctcaaatggc tatgccatta agggggagct tagaagatgg tcatatgatt accaaaagga
    31981 aggtaaagta tggcacaaat ctttttcaag ccaagaggaa gctatccagg aaaaggagcg
    32041 actttgggaa acagctgctg aaatcaaggt agaagtgaca acgaagttag agcgaacttt
    32101 tgattttgct tactatgtag ctgtaaaacc ggctctcgaa ttaaatgatc tttttcaaga
    32161 acatggctac gcgtatgagg catatggtgt aaagaaacac tatgtaaaaa aagtaccgac
    32221 aaagaaattc tttcaagaaa gagagttttt gatgctctat gaagtgccgt ttacgatcaa
    32281 attaccaaaa taaggaagga gaagtggcat ggctacgaaa aaagaacgat cggaaatcaa
    32341 gaaaaaaaca ataaaactca tgtctttaga aggcattgat tatgaggagt ggctgcatga
    32401 acaacatgta aagttcgtgg acgagcaagg agaaaaaatt cttgatcgat taatggacga
    32461 aaaactgcaa caaacgaaaa aaagtgacgt aggtcaacca acacaaatta tccatggagg
    32521 ggaagaaaaa tgacaaaaaa gagaacaggc tatcaatggt taggagtggc ttttgtcctt
    32581 tgggcaatgg cctttttatt ttttacagtt gatttgactg cttttgcgga tgttcaaacg
    32641 acactgaaag atattgaaac tggcgcaggt acacaattca aagcaatcgc aaatatcgca
    32701 atcgggatcg ttgtcattct ttttggtggg gctcaatttt taggagcaga aatcaagtca
    32761 tgggcgaaaa aattattgat tggtgcgatt gtaggtgcta tcgtaattgt caattttgac
    32821 tggatcagag acactttctg gggctggatt ggaggataac caatggtaga agtggagatt
    32881 cttaacgcac aaatcaaggg ctttacactt cctcttgttc ctgtagaatg ggaagtattt
    32941 ttggaggtaa cagaagcaat gaatccttca gaaatgcttt cttttcagct tctggaagtg
    33001 ggtggagcgt ctttttatca aggaaaattt gaaaaacagt caagccttga gtctttcgaa
    33061 gcggatctaa agaaaaaact aaaacttttg gtgaaaaaga agggaatcac aaaagaggaa
    33121 gcgcaacaga ttcttgaact gactggattt attgacaaga agccttcatt atccacaaag
    33181 caaaaggacg aagctgcttt cgctaaaatc tctaaatcaa gtagaaaagg gaatatttca
    33241 ttaaatcaac ggtcaaagtt tttggataag acgtttctaa cgtacatact tgttggaatt
    33301 gccctgttct ttttaatagg agctggtgtt tattttttta aagatcgact ttttcaggag
    33361 aagaggggtc aacatcttcc attgcttcga gtgttgaaac aatagaaatt accctagata
    33421 actatatgga aattgcagaa aacgattcgg ctagacaaga ggaagttgtg aattttttag
    33481 ttgaacaaga agcatttgac cagttggaag catttcaaga agcttatccg actgagattg
    33541 gtaagtttga agtagcattt aatcaaaaag attggcagac ggtgatagag gcggatattt
    33601 ctacactaaa caatgcgcgg aaaatgatga tggcaatcgc gttgattcaa ttaggtcaat
    33661 tggatgaggc aaggctaatt agtgaaagct tgggagatac acgtttgttt atgcagctag
    33721 ctgttgaata tttacggcag aaaaacgtgg aggaagcaaa gaagatccag gaccaattaa
    33781 acgatgatca gttggcagaa atgattgata caacggagat ctatcttcaa atgatcactc
    33841 actatgccaa agaaaaagac tcaaaaaacc aagaattgtg ggaaagaaaa ctcgagcaaa
    33901 ttggtaaaga gtacctcccc caataatatg aaaaaagatg gaggagaggt aaacatgtta
    33961 acaaagatcc gcgactatcg agcgcaaaga aaagtaagga aatggaatag atcgctagct
    34021 caagagaata atacagccct tgctatgggc aaaggagtcc gttaccacca taaaaatctt
    34081 aatcggatta tttcaggaca cgcacaacat cctaagaaga aaataaacgg gtatgatcca
    34141 actgacgagg tactagagca ctcccttgtt cttgactcaa gtagaaacgg taaattttat
    34201 caaacagata ttactcagtg gggatctgtg gatccatttt attcaaaatc ttcctttctt
    34261 ttggtggatg gaatctgccg ctatctagaa aaacaatatg accaagtgat ttcagaagcc
    34321 cttttggtgc aagagttgat cgtccttttt aaagaaaaaa atcaacaaaa cgtcaatgct
    34381 gatgtcattt ggaaaaaaat aatagaagcg tccgattata gctcaattgt tttagatgta
    34441 ataaaagaac cccttcttga agaagtgttt gctttcttaa atgctcaaat tgtcactcaa
    34501 ttggctgaag gggcaataaa aaaagacgag acattttccc caagaataac ggtattattt
    34561 gattctctaa gcatgaagta tcactgaagg ggatggggga ttctacggaa aatagtttgt
    34621 tttattgagc agacgtgaca tgccaatcaa ttagttggta aatccataaa aaaattcagg
    34681 acatattgat aaaaccctat gatagaattg aggtgattta attatattcc tattaggagg
    34741 atttttatta ttggtaagca tactcattaa aatatatgac tctctcaaga aaagtaaagt
    34801 aggaatagct ttactactta ttttagcgat tctcatagca acgactgtta tggtttttgg
    34861 tttgagtaaa ataatctatc atatcgctga tagaccagta ttattattga cagaagtaat
    34921 ctatcttgtt tatgaattgt tgagagcgcc ttatccactt gaatatgatc catatatttc
    34981 attaattgtt tctcttttta ttatgttgat agggtatgtg tctttagtcc taatgactga
    35041 aaaaaaagga gaaaaacgct atttcctttt taaagaaagt catatatatg ttaatttatc
    35101 agaaatgaaa cagttctata tttttatttc tttaacaata ggcttgacgt acttctcttt
    35161 gtactactat tcatttcctc tatcttttga ttcagagagg atacaaatct ttttatggtt
    35221 aggaatagtt ttgggaatat tatggatact attggctgtg caacaatata tatttatctt
    35281 tgaattttta ttttgttata acagcttagc tgctataaga aacaaacatt tttgtaatcg
    35341 actgtgtaac tcactagaga atgatggaga attcaaattg tctgaagcgg gtgtaaatac
    35401 tgattttaga gattgtattg agaaaaatct tataaaaata gtcaatgatg actcactatc
    35461 gaatctgaag tctaatagta aaatctatat tcattttata aaaccatttg atcaaatttg
    35521 taaagataga gaaaacatct tatctgaggg tctcttttta gattattact ttgagtaggg
    35581 atcagatagt aataagtttg tatcgaaaaa atttaatatt gatgatcaaa gacagatgaa
    35641 caaattcatt tgtctttttt gtttgaagaa tggatgagag aggaggtcaa tatgacaaag
    35701 aaaaataaac agaaagaaat aagcaaagat cacaaaaaga attacgaaaa agaaaagaag
    35761 aatagcctac gctttttagc caatcgtaaa tttcttacat atttttctgt gatcgctttt
    35821 ttagtgttaa cgacattagg aaattggctt tttcatcgat gtgtagcttt ttttagtcag
    35881 tcactttttg aatttacagt ggaacgatca catgtttcgt atggtcaaac gatattctat
    35941 tacccaatat tgcgctatcc attcttctat ttcttattat ttatagggat agttactttc
    36001 attggttact ttgattataa gctgtacaca tcttttcgac cattggaaga cgatttaaca
    36061 caaggaacca tgcattttac cccagaggaa gatatggaaa aagaatatga gaaagttcct
    36121 gttcatattt ttgctgaaga tgaagatttt tatgatggaa agccagggta tccggtaggt
    36181 cgtaaaccgc aaacacctga agagaaagaa caaggcatat tttactatta tgtagatacg
    36241 tccgattcga actcggcgac ccttgccggt acaaggcaag ggaaaggcat tttctttgtt
    36301 gatcctttca tagatatatt aactagagcc cgcttaattg ccaatcgggc ttcttttgtc
    36361 atgactgcga ccaaaggaga cgagccgaga aaatggtaca aaacactgaa acgacgagga
    36421 tacaacgttc gaatctgtaa tactgtaaat caatatttct cagacccaat cccaacttta
    36481 gccgtcttca ataattatta tcggcactac aaacgattga agaagaaggc agacaaagcg
    36541 aaacaagaag aggataaagc gagtcgcatg aagtacaacg tattggcaga acaacaatta
    36601 gccaatgcag aaaaagcaat caaccaagct tcattccttt atttcaaaga agtaaatgaa
    36661 ggaaaagacg gtggtttttg gacgaaagct tgtcgcaact tgtttatttc tactggtctt
    36721 gctttagcgg atcaagagtt tgaacaaaat gaaaaaatga aggtgaatcc atacaccatt
    36781 tatacggtag tcaatgacat gatgtctaaa catatcaaag aaggcagcca tgattttttg
    36841 aatcagttcg caaaggataa aaaagaccta cagcgcttgt tagctgaata tgaagataaa
    36901 tcatacttgg atgtcttttt tagcgaaatg ccaagaagtc accccgcaaa gcgctatttt
    36961 gatgcaatca aagcctctgc tccggcacaa gttactctag gaaatatcgt gacacatttt
    37021 gatggagatc tccaacaatt tctcatgagt gctaacgcaa aaatgactgc ttatgatgat
    37081 ggctttgatc tggaatcaat tggttttgat cgtgaacgtc cgactgcagt ctttgttgtt
    37141 ttatccgacg cagattcttc taataatgca gtaggggtca attatattga tcaaatttat
    37201 caagtgttac tcaatcgttg caacttagag gatgattcgg cttgttaccg agcagttcac
    37261 tttatttacg aagaaggcgg aaatctaggt gtggcaattt ctgatttagc tcgcaagtgg
    37321 acttcagggg cgagtcggaa attatttcag catttggtct tacaagatac agaacaatta
    37381 acgtcgttat acgatgagca gaccaaagaa atcattttag ggaatacagc gaatctcgtg
    37441 tatatccgga ctggttccaa gacaaccaat gaatatatct ctggacgttt aggaacccgc
    37501 tcaaattatt cgaaaacacg gcacaaagat ccaacatcga tcagctctac tgaaacagaa
    37561 agctcggaac gaatcgatct gttagctagt tttgaattgg agagaatgag agccggtgaa
    37621 tccgtcgttc ttcgaatcaa taagcataaa gatttaaacg gaaatccaat ctatcagtat
    37681 cccattctaa attctcagga aaatgattca aatatgattc ctttttatga ataccggaag
    37741 ttggataaag tctcttggga ggaaatacct gtaaataacc aatttgtaga aatggaacta
    37801 gaagagctgg attggtcact catgccacaa caggaaaaag caactcaacc agctgtgata
    37861 caaaaagagg tgccacctat tgaaatgtac aagaaaccta ttcctaaaaa ggaggggacg
    37921 caattgtctt tattgtcact ggaagaattg atccagttag aaaaaaacag tgggaaggaa
    37981 gaacggcgga aaaaagaaga aaaagcaaaa gtcttagaac ggctatattc agtcgaagaa
    38041 aggaagcaat cggttcaaac gtattactca aaggaacaag tggaagaaat acagaaaata
    38101 atggctcgct tttttgttgg ccaaaaagaa gtgttacaag aactgaaaca aatacttgaa
    38161 aatggccagg tacagtcttt atgtacgttc ttgttatcga ctagtgaaga acgcatgatc
    38221 aaccggtata tccaacaatt tcgagaatgg aaggaggcag cctatgagtg acaaagaaat
    38281 tttagaaaag ctgctcgaat ttgcgccatt cttgagtatt caaagtatga tcaaagattt
    38341 tggtcgttcg atactttatt ggatcactag tgtgttgatg tggatcgttg aaaaagtagg
    38401 tggggtactg acggaaatgg ttagtgtctt aggcttttac aacgctacgg atatgactgg
    38461 aaaaggggga atcctggata cttttagtgc gtttcaaaaa tttggaattg ggatcacact
    38521 tttaattatc ggtgctgtgc tgtttcttgg gaaaacagaa gaaacaagag cggttccgat
    38581 aaatgcttta ttgatggtag ttatcgttat gttacttcct tcaatcatgt cagatggaat
    38641 gaagatcgtc aattctactg tcgatgcagt tagcagcgaa caagccgagt tcggctttga
    38701 cagctttaaa aagaatgtga ctgatgttta tgttttagca gacaatggtt ggactacaac
    38761 ggaaccaaaa aataaaaatt acttagaaga ccgcgatttt tttgatatta atgagaaaat
    38821 caccgacgct gaagagattg ataatggcga accattattg tatgaagtgg taaatagcaa
    38881 atccggtagt gggaaagagc ttcaaaagtt tgattctgat ggaggaggaa tcactgattg
    38941 gcttatgaag aaattatttg tgaagggtta ctatcggtgg tctgtaaact ggttaccatt
    39001 gatcgccact ctggcatgtc tggcattttc tttagtcatt tctttggttc ggtgtggaag
    39061 gttaggaatt gaacttggct ttaattatat ttgggcgaat attgttgctt acttcagtat
    39121 tcgaaacatt aaattcctca aaactgctgt tttggagctt ttttcaggat ttgtcctgat
    39181 cctttctatt ttcggcatgt atgcagtgtt tattgcatat aattcctggc tagggataca
    39241 acaattatcc gtattagctc agatatttgg gattatcgga ggtgcatggt ttatctatga
    39301 tggtccttcg attatccaaa aaacacttgg tattgatgct ggactatcta cagctggaag
    39361 tttcattatg gggattggtg cattgaaagc cgtgaagaaa gcaggtgaaa tgactggtaa
    39421 aacagttaat ggtgctgcta atacagctgg ttttgtgaaa ggattgactg aaggaatgcc
    39481 ttcaaaagaa gaacctaaaa atgatgaaaa agctggttta aattcagaaa tggcgaagaa
    39541 taccaatgag gatcttccag aagaaaacaa gaatggcagt aacaaccaac aaccggatgt
    39601 gcaaaatcaa gaagattcga atgaaaattc cgaagaactc gatcaacttc aagggaatga
    39661 gccaagtaca agggaagcag aaacagagca agaaacaaaa gaagatcaat taccacaaac
    39721 gccggaaaat caatcagttg aacttcaaga agagccagaa gctcgtattg aagaaaatct
    39781 tgaactgtct gaaaatgatg cgaacttgga acaaactgag ccgtatgcga atgattacga
    39841 aaataatatg gaggatgatc acaataacaa tagtgtagat tactttgaag gggaatccag
    39901 cggttcacca catgttgaag aacaaatacc actaacagat caaccagaaa gcaacaatga
    39961 tcaatcaaat ctatcgacaa gtgacaatga agtgccaatt aatgagtcaa ctgcgaccag
    40021 tttaaattca ggtgatgttg aatctaaaaa gatagggaat gagaaggatt cagctgtgaa
    40081 aaataattct atgactaaga atatccacgg aaaccatgtg gaaaataaaa agccggaaaa
    40141 tgataaagat gaagaattac cgaatccatt tgttaaaaaa gtgaatcaaa tgatccatac
    40201 gccaaattac caaaagaata cgaaaaataa ggtcgggaac atggttgatg ggtacaatcg
    40261 caacaaagaa tttggccagg aagtcaatga atggttaaat caacgcagaa ataaacgaga
    40321 agaggaacat cctgaatcag ttgcccctta tctagaaaga aaaagagcta aaaataagaa
    40381 taaatcgggg taggtgaaaa cagtatgcac gatattcaaa acgagatatt tacggaaacg
    40441 aaattttggg gcgctatttt attaaaagat agcgtagcag gttttttaat ctttaatctt
    40501 gcgagaaatg tccaggatgc gacttatggc ccattacaag gcgtttcttt ttggtttgtc
    40561 attttgatgg gctttttttg gttatgtccg agtgtgagta atgcgaaact gaggaattac
    40621 caacgactat ttattacttt aacaaataat cgatcgacct accatgcgat cgatcgaatg
    40681 gattttgagg tacaagaacg aatcgacatg gttatgggaa ctgtagaaga aatggaaaaa
    40741 gaagacgaaa tgctttcttt agaagaactt gaaactgagc ggttggataa cgtgtaattt
    40801 agggaagtgt ttgtggtata ataacagaac agtaaagttt gtgacggtgg ctatcttttg
    40861 gaaggtggtg gtgcttatgg aagtaagtgt tcactcttta gaaaggagaa gccttttgtc
    40921 tgtagctgaa gcattaggat taatgattag tttcggttct ttaatcgcaa caatcatttt
    40981 cggtatccta actgcagtga aaaatgacaa gaaaaaataa ccgtctactt tagcgagtca
    41041 gcggttattt tttagatata actaatgtcg ccaccgtctt aagcggtgct gtaggggagt
    41101 tgttgtcgca actcctcttt gtatggttat cataccataa tctagctttt cattcaaatc
    41161 atggtgattt atagacgttt cattgactac ttgttgatga agcgtctttt tttacaaaat
    41221 agaggcacaa atgaaggaaa ggattcattc aaaaacgtta cgaaaggaat ttgatgaaca
    41281 tgaaacgacc gtcttatttt tgatttgtat cgaagaaaca taagcgatcg tacggataaa
    41341 tgaatggggt gtattaattg agtgaaagta taaaaagttt ggttcagtca aaaggtttct
    41401 ggaaaattca attgttaaat gctttatggt tgggaagata ttatttccgt gtttgttgta
    41461 aagtaatcgc tagtatgatt ttgattaatt ttttctttga gggggtggcg ttgacgtcgg
    41521 atcagaaaca tttgtttctt gaactagtat tcaatttgtt ttttatgatg atgggtctga
    41581 tgcttattct taaaagtatt gagtttatca caaaacgtac gactgacatt aaaagtttta
    41641 tagtagtggt actaagattt tctattttca tgatgatctt tgtacaaatc agtggactgg
    41701 aggttaaaac ctatgattct attttacaag gattcaatca aaataatttc gattacttga
    41761 tcgatcacta tggtcgatat atttgctttg cgtgtttgta tttagtttct tataagtttt
    41821 attatgaacg cctctttgtg agtgctatag atgtcaattc gtttaagggt aatcacttat
    41881 cctggaatgc tagtgactgg ctagtttgtt ttgtttctcc taactatata agattagaaa
    41941 aaaagggaag gataaaagta cagttttgtt tggattattc ttggattaga ataaatcact
    42001 ctcactcttt agaaactatt gaacagtttg taccagaaaa aatgagaaga tgaagactcc
    42061 gtttatggta taataacaga acagtaaagt ttaatgacgg tggctatctt ttggaaggtg
    42121 gtggtgctta tggaagtaag tgctcactct ttagaaagga gaagcctttt gtctgtagct
    42181 gaagcattag gattaatgat tagtttcggt tctttaatcg caacactcat ttttggtatc
    42241 ctaactgtag caaaaaatga caagaaaaaa taaccgtcag ctttagcagg tcagcggtta
    42301 tttttaactt aactatctgc caccgtctta agcggtgctg taggggagtt gttagcgcaa
    42361 ctcctcttta catagtcata gtaacacaat tgtccctatg attcaaatag ataagatagt
    42421 aggcgtttca ttgactgata gttgatggaa cgcctttttt gtaactaatg gaggtgctaa
    42481 ggaagaaaat gattgattca gaagcgttat taaaagaatt agacaaacaa gaaacgatcc
    42541 ctaaagtagt aaaagatccc ttacttgagg aaaaaccgaa gaaagtccgg cgattgaaac
    42601 aagaaaaaac tgttttatcc ctgattccct atcggagaat catatcagaa gattgttttg
    42661 cgatggaaaa agaagtcaca aatttttacc gaatcgaaga tgcttctgca cgtgatggaa
    42721 gtgaacaaca gcgtcaagag gcgttgcacg attacctggt attcttgcgt accgtccaca
    42781 agaatcttaa attgattttc accagctttc cggtcgatat gtcggaaaac attgactatg
    42841 cacgccggcg ctttaagatg cgaaaaggaa ataaaaatgt acaagcagaa caggcaatca
    42901 cccttagtca gttggagtat ttagataata ataagccaat caacacggtt tacttacaaa
    42961 tattcgccac agataaagac gaattaaaag atgttgaacg agaaatcatg agtgtttcta
    43021 cggcaaattt ccgacctgtc aaaatgtcat taactcaaaa aatcaagcta cttgtacgtt
    43081 tgtataatcc tttatcaacg attccaattg gcattcctta ccaggataca gatgaaacgg
    43141 gaagaagacc ggatgtccaa aaaatcgtgg ataaaaaagg gtacgatcca ctatttatcg
    43201 gtcaaattca accacttggg ggattaagac catactctcc aacggaatta caaatcggta
    43261 atgggttcat tcgtgtattg aatctactcg tttatcggcc gaaaaataat ccgaactttt
    43321 gggggcaaca catttttaat atgaaaaacg cagttacgac attagatatg cgatcgatcg
    43381 atgtgacgaa tcctttggta gagaaaaatc tgaataattc cttagaagaa tacgaagatc
    43441 aagtcgtcac gtcaaaatcc agaatatcga agaaaaaggc gaagaacgaa tactacaaat
    43501 tagatcaaac ggttgaacag attcttgaag gacaagaggt gctaaaagaa attcatgtcc
    43561 gatatatcct cagtgcagaa tcaatagaga aactgaatga aaaggaaaaa gaagtgcata
    43621 ggcaattagg gaaacgacaa tttcaggcgt cttgcttctt agacgaacaa gaacgccaat
    43681 ttaaagcttc ctttgtttca tttgcgcaag gtagaaaact cattcgaaga aaaggaaaag
    43741 agattaaagg gtctgcgctt gccggcagtt atccgttcca tttcacgaat catatcgatc
    43801 cagacgctcc ttattctggc tatccgcttt atggatccgg aattatttgt ctttcaccta
    43861 cccataagga tcatcagaga aaatcctata gcatgatgat cattgggagt caggggtttg
    43921 ggaaaacgac ggtttctaag aaactagcga aacaatttgt ggaatatggc aataatcatt
    43981 ttggttttta tatgtctgat gaaaccaagc gtttgacgga atacctgggg ggaatccatt
    44041 tagatggcgc agaagcgcgg ttaaatcctt gtcagattta cgcttcagat gtggacgcaa
    44101 aaacaaagaa aacgaaagaa caacagtcct atttaacaaa tctaacgaaa atgcgcttga
    44161 tttttgcctt gaacaacgaa ataaagagtg atgatccagt gatggtggag gcgaaaaaat
    44221 tatttcccaa agcctatgcc aagtatatgg aagatcacaa ccttgattta tcaaaaatta
    44281 ctcaatatgc ccccgaacag tatccaatct attcggatat tttagacttt gcaaagaaag
    44341 aacaagcaag agaagtggaa gaattccgaa aaaaaagctt gtatcaattg gtatcgcaac
    44401 tggaatcttt tattgataac gcaggaaata tctttaatca acgatcagaa tttgatttta
    44461 gtcaccggca gttagtgacg ttcgatatgc aaaacttgat caaatcggat cgagccacct
    44521 acaatgcgca gtattacaac ctatatactg ccgctttcag tgaagcggtg cgtgtaggac
    44581 agcgagaaaa gtatctcttt gaccggaaac aaaaacgagt agatgagttg atctacacga
    44641 atattacttc tgatgagttc cataatccaa ttcgaacgga aaatttgacg ctgttaaatg
    44701 aattagaccg ctataaccgt gaagcgcgta aattattgat tgggcaaaca tttattttgc
    44761 atgatattgc ggatgctttt ccggattata cggacggaaa gatgggggaa atctctaaag
    44821 cggtgatgaa tctgttcaaa ttgactactt atcggttttt attgcaacaa gatagttctt
    44881 cagaagaatt aatgaaaaaa gtctttggga agcaattaag caattatgaa ttagcagaga
    44941 tttttaggtt cgaagaacgg gatattctgt taaacattaa gggatctacg aatattaagt
    45001 ttcgctacga cctaaccgaa cgtgagaaag caatctttga tggaggtcta taatgaagaa
    45061 caaaaatgag aagaaatcgc tgaagaaaga gaagcgagct agacattggc agaagacgaa
    45121 aaagctgaca agacggatcg tcattggtaa accaggcgaa aagatccaaa caacgaagac
    45181 aaagaccctg tatctcattg tggtcattgt cttttttctt tatctgttat tgcagctgtt
    45241 cgtttaagta ggattggagt gatctaagtg agccaaataa aagtttttac ggaaaagcaa
    45301 ttgaattatg cacgtaagac caatatgctt tcttatatgc ttgaacgagg agaaccgttt
    45361 aggcaagtgt cctcaaaatt cgtcgaacac agggaacatg atagtttacg agccaatata
    45421 aagaccggtg tacttaactg gtattcacaa aatctaacgt cttggaataa tgccattgaa
    45481 ttctcaatgg ctttttttaa tgaatccttt gaaacaactg tagaagatct tttacagttt
    45541 caacggacac agcgtgtaat ggatcggtcg acaaatcgat cggtcaccaa taaaaacgtc
    45601 ttagaaaaca aactagagcc atttacgctt acccatttat caactatggg aaattatgat
    45661 acgaatgtgc ttagccaaaa aggccgtgag tatttacggt cccgttacct gtcggatgaa
    45721 acaatcggct atttagaaaa attaaatctt gtttctactg ataacaaaaa taacctatta
    45781 ttcaagcata ttgatatagg aatcggaaaa actggaaaca ttgtagggtg tgatatacaa
    45841 ggaacgtatg cacggtctct tgataaacga gtctcactca atgataaagg aaaattgaaa
    45901 ttagaccgta aatattttaa aggcatgggc gaaaactcca tgagcaagcg tggcttttta
    45961 tttggcgtga acgtagccta tagtgttccg ttgactttat ttgtgacaga aggccccatc
    46021 gaatctgctt cattgatgga attacaaaaa gaatctttgc ctatcaatac ttggttttta
    46081 tctctttctg gtttgaaaga agatacattt tgggaggtaa aagctctatt acaagagctg
    46141 actaaagcgc caaaagtaga aagtattctt gccgtaaata acgatgaaaa aggccaagaa
    46201 tttgttcaaa atgttcacga aacctatcaa gacgaaaaag agtaccagtt cgcccacaca
    46261 ctcaaattac ttcttcctga attggaaaat ggggattgga atgagcaatt agagctttta
    46321 aaaactggtg tcttaaatag tcgttctgaa aagttaaaag aaaaacgaga agcagaaaaa
    46381 ctggttcagt cgatgcagct atcgtacatg gaaagggttt aatcatgaga ggactaaaga
    46441 tcaaactcct agtggttgtg cttctttctg tgtttacttt ctcagcagta gagatggtta
    46501 tagaagaaac acaaaacatg ttagctggaa ttttaggtat cgaacagacg agtcagaccg
    46561 ttgaaatcag cggaaataag gcgctctcag cggaagtctt agcgttaaaa tcaatcgttg
    46621 aaaaagaagc gcaagcagcc gaaattccct cggaagtgcc ctttctactt gcgatcatga
    46681 tggtggaatc aggcggtagg gggagtgatc cgatgcaatc aagtgaatca ggaggactgt
    46741 cgccaaatag tatttcaagt gttgaatggt cgattcgtca aggagtaaag cactataaga
    46801 ccaccttgga attggcgaaa agatatggac tggaaaatga taagaaagca atcgttcaag
    46861 cgtataattt tggatctagt tacgtctctt ggttaggcaa tagtaagaaa aaacattctg
    46921 tggatgaagc ggaaacctat tcaaggacgg tggtcgcacc aagccaacgt accaatgggt
    46981 atcccaatgc cggtcaaaag tattcatacg tcaatgaagt atcaaaagca gatgggcgaa
    47041 cgtatctgta tcggcattca gggaacttct tttatgccga acttgtttac caatacattg
    47101 ataatacaga aagcagaaaa tcttcgaatg gtgcctatgt tttgccagtg gataatccag
    47161 tgatttcgag tggatttggt tggcgatcgg atccttttgg aggcggaaca gcctttcaca
    47221 gagggctgga ttttgcgaat ccttttggtt caaatatcaa agcgattgcg gctggaaaag
    47281 taatcacagc cggtccacat tatagttggg ggaaccatat agttatccaa catgagaatg
    47341 gtcaagtgag cctttatgcg catttaaaca aaattttggc aacagttgga cagactgtca
    47401 aacaagggca aacaatcgga cagcttggta gtacaggcaa ctcaactgga ccgcatttac
    47461 atttagaaat tgcaaaatca agtgatttat ctcaagcgaa tttgatcgat ccggccgtag
    47521 ctttgggatt gaaataacag gagaggaagt gtatgaatgg aacgaaaaaa gagtgttatt
    47581 ttggttattt tagtaagcgt aatgagtttt attgcaggta gcttgattgc attacattat
    47641 cttcaaccag ttctaaaaga aagggaaaat aatacgttgt tgtctgaaca aaaagcaaat
    47701 caggaaacga taaattctta tgaaaaagaa aataaattgt tgcgagaaga acttgattca
    47761 caggaagtga atcttccaaa agacacacga tcggaagtag aattcaagaa tattgagaaa
    47821 gtagctaagg cttttacaga tggtttctat acaaataatt cagataatat tcaagagaaa
    47881 atggatcaga ttcaagagta tcttacgcca aaagccaaag ggaaaatcgt tccctattgg
    47941 gtagaaagta acgacaaact gacggttgaa caaagcaaaa tcagtgaaaa atattacgtg
    48001 aatttagaaa atgtattagg cgaagcaact gtgacgggct tcttatatgt gtcgacaagt
    48061 tatgaaggag acaagccctt taccgttcgt tacctgatac aaatggatct gaaaaagaat
    48121 gaaaataatg tctggcaagt gaatgaactc cgagaaacag tcaccacgaa tgatgtgccg
    48181 gaacgattct atgactagag gaggatggca caaatgaaga aaatgaacga actgattgaa
    48241 cggattgaaa ccttcagaga ggacttccaa aaagggcact ttcagaagtc aaaagtgaaa
    48301 caaaaacgtg cgaaattgtc acccattatt tatcgaggaa tgtttctact tacgatcgta
    48361 ggaactcttt ttttcttcag ttcacgtctt ctttttgaag acgatcgtcc aattatagat
    48421 agtggtatcg gaggcaaaag cagccaaact atcggcagtg atacagttca aatctatcaa
    48481 cggacgtata atccgaaaac aaattacatg gaagtgattt tcttaacaag tcgaaatgag
    48541 acgagtgtga atcgagcgta tcaagcattt gttgggtctg tgaaagaagg caataaaggg
    48601 actgctttag cgtccaaatt agaacccctg tatgacaatt attatctttt aactattcca
    48661 gaagttcctc aaaagtggac aacacttttg atcgattttg gttattcaga aaaagagatc
    48721 aatgtgaatc ctacctacaa tcaagaagga gaaatggtgt ttgaagatac ggaagaagtg
    48781 aaccaaacag tcttttacat tgattatcgc aaagtgaaaa atagttccca acttaagaag
    48841 aaaaagttga atgattatgt gatcgagttg accgaaaaag acattgaaaa ggcaatcgaa
    48901 gagcggaaaa acttgcagga tagtattcaa aaaattgaga cagaaatcga gaaaacgaaa
    48961 tcaaaaatct tagaaaatga aacgtcaatg gagtatctta cggcagggga aaaagaagag
    49021 atagagaacg caaataagca gttaagtagt caaattgata gctattccaa cgcaattgaa
    49081 gaagcgcaaa aacaaattga ggtgattgaa gaacgtcgtc aaaaattgat cgatcgaaac
    49141 cagtctgcgc aagtggaaac cgaaaataaa taacaataaa aggaggggcg actcaggggc
    49201 gaataggggc gaaccaaggg tgcgaaatga aagaaattgg ggcgaacagg gggcgaattt
    49261 ctaaaaaaga cgggaaaaca ggggtgaacc aggggcgaaa acactgaaaa ttaagggtga
    49321 ctcaggggcg aaaaagtgca aaagtgcatt gcacagaaaa gccttgcact gcaaggcatt
    49381 acaccttgtg tgtaatccat tttcccttat gctcttacac tagacagagg cgaataagcc
    49441 aatgttaaga cctagagggg aaaatcgggc ctcagctggg tataaaaaaa caaggggaga
    49501 caatgatgag ccgaacaaaa ccattacaaa ttcgtgatgt agatgaagaa atccatcaaa
    49561 gattagttaa aatagcaaag aaaaaaggat acaaaagtcg tgatgatatg ttgcgagaag
    49621 tgttaacgca acttgcttat gatgagttcc agttggatag tgaaatccgg tatcgacaat
    49681 tcatagaaaa gcaaaaacaa ttcatggaat ggttagctat aactgtcgtt gaaaaatcct
    49741 attcggaaat agaaaaagag ccgttcacag aataaagata ggagagcaga ggcatggaac
    49801 aagaaaattt agaacaagaa aacctgacta gtgatccaag taaggataaa ttagaggaag
    49861 aaaaatggag aaagaaaaac tattgggtaa aagaatccac gatcgaacga attcgctatt
    49921 ggaatgaagt gagacatgat aagaatgaag gctatacatt agaggccctg ttggattttt
    49981 atgaaaggaa tcacgctcag gaacaacaag cgttggaaga aatattgact cttcaaaggc
    50041 aacaaatgac tatcatacgt gaagctgaaa tgaatagtaa agtaaatcaa caattactca
    50101 ataccatgat ctggtttcat gagattgaga gcttccagcc aactgattct gtgccactta
    50161 ttgaagcgaa aaagtatcta caagaattac aagaaagtca aattaaaaca gcgcaccacc
    50221 ggaaaaaata tagtcaggtt ggtgatgagt aatggctcgc caaacagctg gcatcattta
    50281 ttcagctgca ttcgttattt ccagcgcaca ggcattcaaa gaattcattg attatatgaa
    50341 tcgaagtgca gcagttcgta acgagaactt tgataaatac aacgcatttt cagcacagaa
    50401 tcaagcggat agttacaaca gtattgaagg tggaaatgaa acgatgttct ctacctataa
    50461 cgattatatg tcgaatcccg aaaagacaag ctcgtctttt actcgtcact atgatcaagc
    50521 accaatcgaa gtgatgaatc acctaaagga ttactttacg caagcgcagg aaaatcaatc
    50581 gccgctttgg cagatggttt ttagtttcaa aaatgagtgg ttggtagaac atggtttgat
    50641 gaacgaaaaa acgaatcgag tggatactgt ttcactgatc gacgcggcaa gaaaagctgt
    50701 cactgattta gaaaaaaaag aaggattaga aggagaatgg tcgggtgcca ttcactacaa
    50761 tacgaaacat atccatgtcc atattggtta tgtagaaaaa gaaccaacga gggagtgggg
    50821 atattatgaa cacccaactg atccagagaa aaccggttgg cagtatatgg ccaaattcaa
    50881 acagtccgcc ataaaaagta cacgtcgcac gtttgtaaat acgcttctca aaaatgaatc
    50941 gcaattgaaa gaattgagtc aatcaatacg gcaacaagtt gcgttggcta aagagaatcg
    51001 tagtgagttt ttggcaggtc gatacgaaat gttagcgacc gatttactgg aagcattgcc
    51061 tgataacaaa tacttttgga aatatggatt tgcccaaagg catggcttta aagaaccgct
    51121 cgatcggatg gtcaatttgt tcttaaacac tgacggaaaa gaaacgatgg aaaaagtgat
    51181 tcgcctttta aaaccaattt ctgacgaata cgaatcagcg tatggaaatc caagaaacgc
    51241 accgacgtat tacgagaatc agttgtatgg caaaaatggg ttatacgctc agattggaaa
    51301 ccaaattttg catgaggtaa gaacatttgt gaagagtcaa caaatgcaaa aagcagtggg
    51361 aaatatgact gcagaagaat ttgaagctct tcaaaaagaa caagcggttt tgccaattga
    51421 tttaggatct ccaacagaac aagatttcat tgttccggaa atggaagatg tatttcactt
    51481 gttaaatgag ataccgccac cacttgatca cgaggaagtt gaacagccag tcagccatag
    51541 tatattgaaa acggagggga attccgactc tgttgaacga acgaaagcac tctctgaagc
    51601 atttgaattg atgaaacagc actttgacaa gcgagatgaa tccttagacc aacgtcacaa
    51661 gggcatacag tacaagttaa accaagagaa cgccaatcgg gaggatacga tcgagcgaag
    51721 agcgatgtac attcatgcgg tagatagtga gacggatttt gttcactatg aaccaacaag
    51781 tattcaaaaa cataaaacgt tggatgaaga attcgtatca gcaaaagaac aaaaatcatc
    51841 tgagagcgag gaaggtaacg agcaacacaa tcttgatcga ttttcaaaaa agaaccgcac
    51901 gatgatcctg agtcaaaacc ctggggctac gtttgtttat gggccccaac aatggaaact
    51961 aaatggccgt aaggtgcgtg atgacgagat caatcaaccg ttggttatca ttgcgcccta
    52021 caaaatagaa gacacagtcg tctttgaaga aatttgtgtt tacaatatta gtcaaacaga
    52081 agcctataaa ccaacagctg cagaactcga agagctgtta aaaaaagaaa ccgcaaaggg
    52141 gaaaagcatg gctccaagaa gtgtttctcg attccaagat cgtcgcaacg aaatggaaat
    52201 gcgaaagatg gttcaatcat taaggagatc ttgcgaaaac agtctgcagc attatctaaa
    52261 tgaacgagca tatcgtgaac tgcagtatga gagtcaatca aatatttaac aaaacaataa
    52321 gaaagaatag acggtttggc ggaagtgcct gaccgttttt taggaggaaa attatggttc
    52381 agacagatac tggagatcaa ggaatagatg ttagaaaaga acaacaaaag attgtcgctt
    52441 attttgaaaa agatcccact gtaaatcttt gggtaactcc tgatgaaaat ggacaactgt
    52501 atgttgtgtg tgaatcggaa gatgattttt taaagactta cttgtatgaa gatgtcgaaa
    52561 attttattga tgcgaaccct gaaaaacatg gttcttcgtt tcagctattt gaatggattg
    52621 accattcagt ttctgaattt gaagcgtcgg aagatatcga tcatggagat gaggaaatga
    52681 ttatcgaaga aaaacaaatg attgataaaa gcaaaaagaa gcggaaagaa aaagcacaag
    52741 gattggaaat gacttaggaa aattttgatt caataataac atgttgtata cgattagact
    52801 tatgttataa tataaatata agtctaattg tatataagag ggcggttgaa ttgaaaaaag
    52861 ttgtttatga gctacttgag caatataatg ggacattggc tatgaaggat gcaaaaaaag
    52921 caggcgtttc accggttact ataaagcgaa tggttgatcg tggagagtta gacagagaat
    52981 atcctggttt ttttactttg cctggtcaat ttgcagacga actcctcatt gcacaaaaga
    53041 aatatgaacg tgggattatc tctcacatta ctgctttaga tctatatgat ttaactgata
    53101 tgatccctag aaagattgat atgactgttc catatggata tcatgtttcc gaaaaaggat
    53161 tgacggaatt tgcagttgat ttgcacttta cgaaacctga atattatagt gtcggaagaa
    53221 caaaagtcag aagtcgatat ggaaatacac tcgttgcata tgatgttgag cggacgctat
    53281 gtgatatttg gaatccttgg tataaagtca cagatgaaat taggatcaag gcgattaaat
    53341 attatatgaa ttctaaagta agagatctta gcaagctcag aatgtatcaa agaatcctac
    53401 cagtagacaa ggaaatgcgc tcatatatta tggcgttaca ctaaaagtcc aatatatgtc
    53461 tgtcagagtg acaatgaata gaactgacgt ataaatggtt ttatgcgaat tactaaaaat
    53521 ttaagaggtg aaaaaaatga actatggttt ctatgatgaa tattcaatag cacgaaatca
    53581 attccataat cgaaaatttc caatggaata tctagatgat attcttgttc gtcttgctta
    53641 tcattcaagc gcaattgaag gcaatacgat ttcattacca caaactgttt cgatcatact
    53701 tcataatact gttcctgaac gcacgtctct cagagaactt tatgaaattg aaaatcatag
    53761 aggagcgttt gctcttatgc tagaaaaagt gcaagacaaa caaccactct ctatatctct
    53821 agtaaaggat atacacgagc gattgatgga taaactaatc gtggatcgag gacaatttaa
    53881 atcctatgaa aatgcaattc taggggcgga ttttgatacg gcttctccac aagaaactcc
    53941 agtattgatg cagcaattag ttgataatca gaattatcgc ctagaaattg ctgataccga
    54001 tgtcgaaaaa attgaagcaa taatggacac tcacattcaa tttgaaagaa ttcacccgtt
    54061 tagcgatggt aacgggagga ctggtcgatt agttatgaac tattccttgt taaatgaagg
    54121 ctttccacct gtaattattc aaacagaaga caaagccgaa tatttggaat tgttgtcaga
    54181 acaaaacgta aaaggtctga ctcgatacgc aatggaacaa atggtaacag aagaaaaaag
    54241 acaagcaaag tttattgcaa aagaaaaaag tcaaattcaa gaattagatt tttaaaggat
    54301 cagcatagct ggttcttttt tttacaaata aaaactattg tatatctttg gatatacgag
    54361 tataatagcg catgaggtga acagcaatca tgaaagcagt taaaactaga atcattggaa
    54421 actcacttgt catttctttg ccaaaagaat tacaaataaa agaaaatcaa tacttttatt
    54481 gccacaaaaa agaaaatggc attattgagc ttgttccaaa gattgaaaat ccattaaagc
    54541 agataagcga tgaaaagtaa ctggagccaa cgcaacattc tatcagatac cgagggacag
    54601 atgtatttga ttctgagtgc aaatgtcttc tatgagatgt atgaacgagt gatcgtctgc
    54661 aaagtgacgg acgctgcgac tgaagcaccg ttagaggtca aaattagctt agaaccttca
    54721 aaacattcga tgatcgttga ttgttctgaa ttttttacga tcaatccttc tgcagatctg
    54781 tacacgttgt acggcacatg cgatgaaggg gagtttcttc tagttgcaca aacatttctg
    54841 gctatctttg attttctttt ttttgattga gttttaccta tgcgctttac taatttctag
    54901 gaggcgcaca tggacaagaa gaaacaaact actttacgaa tgcttttcaa acaaacttat
    54961 gccgaagctg tggaaacgcc acaatcgttt aagaactatt tagaccaagt aaagtttctt
    55021 cacaagtacg atgtaaacga ccatttattt attcatggtc agaatccaga agctcgctat
    55081 ttagcggatt ttggtacgtg gaaaagaatc ggtcgtcaag taaagcgtgg cagtaaagca
    55141 atcatgacac ttggtttttc aaataaccaa atcaaagcct cgtattactt tgatgtgaat
    55201 cagaccattg ggaaagtgct agattttcca gattacagtt tggaagaata cacgtggcca
    55261 gattttatca cacaagaaag acaggatatg atcgactctt ttacagcaga cttggaagat
    55321 cctgttcata gaagattggc tttagagata ggtttagcaa tggttcagag taaagccttg
    55381 attcagccaa ttcctgaatt tgaagaaatg ccacacttgc aaaattttcg gacgtttgtg
    55441 gaagtgatga ccaaagcaaa cgagatcaac cgatttttaa gtaaagatat tttgatttat
    55501 aaaactgaga ggagtaagga agatgaaatt gacgtatcaa gagaaccaga acggacagct
    55561 gatcccgcaa gtggagtatc cagaaaacca accgctcggt cgttttggct caattgccgt
    55621 ggaaaaactg aaggaagaac agccaacaga gtatcagatg aaactgatgc aaggggaact
    55681 aatgacttac ggccacacta tcgacaagaa ggtatgggag cgagtagcag tactagcgga
    55741 acagatggag caacagaatc cgttgacctt agaggagcag cagaatctgg agacagctac
    55801 caagattcgc aacaactatc gggaacaagc gatcgaacta gcgatgagcg aacttctgta
    55861 aaacaaaatg atctggaaac tggtttggaa gagacaacgt cttctgaacc tttttttgtg
    55921 tctgcagatg agcaaaaaga cagtagtgcc tctttagcag cagatctatt tcctgaatca
    55981 ttgattaccc gtcttttaaa ggacgggact ggaactgttg atggaaagtt ccgtgtttct
    56041 ctagcttttt ctcagacaaa agacatgata cagcgtgttc gttttttgaa aaaagaattt
    56101 ggctggtatg gcaaaacact ggatcttcct ggagcaactt tttcgtccat gaattcacgg
    56161 ccagaccgag gtattcaaat cactaagata gttgacggta aagaatatga gaaagactac
    56221 aaatggattg atattgcagg aagaattgat ttccttattg gtaggaatga ctatttgact
    56281 gcagaagaaa aagagcgagt aaatgaagta ttccaaaaaa cgaacgaact ttctcagccg
    56341 attgatgtaa gtgacccaaa agatgaagcc actgaatcat tggataaggt agaagaaatc
    56401 actctctttg atatggatga ttttgaagcc actgaagaac cgctccaaga cagtgggtac
    56461 ggctctgagg tcgcttttgt tccaatgcta aaagttgaga agccttctgt atccgtcgaa
    56521 aaacaagaaa gccacaacgt gataaccaaa ggcagcaatc acttttcgtt tccgttggaa
    56581 tcgctagatg ccttttatcc aaaagcaact aaagacaaga tacaggccaa cattgatgcg
    56641 attgttttag tcagaaagct cgaatctgaa aacagaatgg ctacggtaga agaacaaaag
    56701 gttctggcga agtacgttgg atggggcggt ttagcgagta tcttcgatac ccgtgatgat
    56761 cgattgaaga acgagcgtga acagctgaaa aaattgttga ctgaagagga atatcgccaa
    56821 gcacgtgaaa gtgtgttaac ggcttactac acagatccac ggctaataga agtgatctat
    56881 gagaagttac agactatggg tttccaggga ggaaatattt tagacccttc tatggggact
    56941 ggaaacttct tttctgctat gcctgaatca ttaagaactc agtccgatct atacggtgtt
    57001 gaattggatc cattgactgg agcgatcgca aaacacttac accaagatgc aaccattgag
    57061 gtgactggtt ttgagaatac ccctctaaat caaaatggct ttgatctcgt aatcagtaac
    57121 attccttttg atagtataca aatcgaagat cctaactacg accatagcta tgccattcac
    57181 gattacttta taaaaaaagc attggatagt gtaaaagaag gcggtattgt agctgttatc
    57241 acgtccactt caacattaga taagcagagt gaaaatgttc ggcgtgagta tgcaaaaaca
    57301 ggtgaattgc ttggtgccgt tcgtttacca agcaatgcgt ttaaaaaaat agcaggtacc
    57361 gatgttgtta ctgaccttat tttctttcaa aagaattcgg aaatcgagcg ctcatataca
    57421 cccagctggg tgtttgtcgg catgcataga gatgtaccag gaaaaccttt taacaactat
    57481 tttgtgaaaa atacggacat gattcttggc gaactttcac ttaagaattt tcgtgggaaa
    57541 accctcgatg tgaaagcaaa tcctgaagcg aatttgattg atgatctaag gactgcgatt
    57601 gaaaagatac caggaacgtt cgagcttacg ccttccgcta aagagatctc tatcgtagaa
    57661 caaagaacag acaatgaagt ttcagtttcc gagatcccga atttcaatta cggccaaagc
    57721 caagggaaaa tctacttcaa caacaatggc tatttagaag tatttgaagg gtcagaaaaa
    57781 gccgaaaaac aactttccca attgatcaaa atcaaacaag agctattaca agtgatttat
    57841 gttcaacgac aagaagatta tagtgtggaa gaatttcagc ttgcactggc aaaactgaat
    57901 caggcgtatg atacctttgt atctgatttt ggtccgattg cgcaaaactc gaaagtgttt
    57961 caacgtgacg aataccttcc attactaaaa tccattgaaa tagtccataa ggacggctct
    58021 gttgaaaaag gggatgtctt ttttaaggca actatccgga gaaaagaacc aattacaaca
    58081 gtcgattctg cgatcgaagc attacagctg tcattaggac ggcgtttaaa agtagattta
    58141 gattttatgg cggatgttta tggaaaagac aaaggaaccc tagtcaaaga actcgaaggt
    58201 caaatattca ttgatccatt gaagtataaa ggggatcaat ttgatgatgt ttgggaagta
    58261 agagaagaat atctaagcgg tgatgtcaaa caaaagctag tagaagctcg gtacttcaat
    58321 gaaacctatc caggactatt tgaaaaaaat gtgtcctctt tagaggacgt gcagcctgag
    58381 ccattgaaag ctggtgaaat cgattattcg gttggaagta cctggatacc taaagaaacg
    58441 tatgaacagt ttatgtttga aacgtttgaa acaccgccgt ggatggttcg ggaacattat
    58501 gtccaattgg attatgatcc aaccgcttct cgctacttta tttctggaaa aagtacacat
    58561 aaaggaccga tcgtcagtaa ccgctacggc acggcacgtg cgaatgcgta tctcattttt
    58621 gaaaatagtt taaatctcca aaaaatcgaa atccgtgatc gagtggaaga cggcgatgga
    58681 aaatcacact atgtattaaa tcctaaagaa acgcaatatg ctcgttcaaa acaagaagaa
    58741 ctgcaagaaa tcttcaagaa ttgggtcatg aatcattctc aagtccttga agaactgcaa
    58801 ggaatttacg aagagcgatt caatcgatta gtaccgagag cctatgatgg tagctaccta
    58861 gagtttgaag ggctgagtga acaaattgaa ttgcgaccgc accaaaagaa tatggttgcc
    58921 cgaattattc aaaacggacg tgggttgatg gctcatgtgg taggcgctgg caagacgtta
    58981 acgatgattt ctgcaggaat gatgatgaaa actcatggtt tgatcaagaa agcgatgtat
    59041 gttgtgccta accacctgac aggcgatttt ggtaccgaac ttttgcggtt ctatccgtcc
    59101 aaaaaagtgc tagttacaac caagaaagac tttgagaaag ccaatcggca acaattcgtt
    59161 tctcgaatcg ctgtcggcga ctatgatgcc gtgattattg gccattctca gtttgaaaaa
    59221 attgccttat ctccagaacg ccaacgaaat atgttaaaag cagaaatcaa tcaggtatct
    59281 gaagcaatcg ctgcttatca aatgcagaat gatgttgatt cctggtcgat caagcagatg
    59341 gttagttttg agaaaaggct caatgaacgg ttagccaaac tgaataaaca agacaagaaa
    59401 gatgatttga tttacttcga ggacttgggg gtcgattttc tgtttgtgga tgaagctcat
    59461 ttatataaaa acctctattc ctacaccaag ttatctaatg tagcaggggt caatgcgtct
    59521 aacagtctaa gatccagtga tatggaaatg aaagtgaagt atatgcttga agaaaatcag
    59581 gggcggggcg ttgtttttgc gacaggaacg ccaatctcta actcgatgtc ggaaatgtat
    59641 accatgcaaa aatacttaca accagatgta ttaaaagact ttggtgttta ccattttgac
    59701 gcttgggctt ccacgtttgg cgaaatcgtc tcttcactgg aaatcacgcc tgaaggcagt
    59761 ggctatcaga tgaagaatcg tttttctaaa ttccacaatc ttccggaact gatgagtatg
    59821 tttaacttaa ttgcggacat tcaaaccaaa gacatgctta atcttcctgt accagcaatc
    59881 aaaggagaga aagcacaaat cattgttact gagcctatta cctatcaaaa agataagatg
    59941 gatcagttgg ctatgcgtgc ggaaaaaatc aggaatcgtg aagtgtctcc ggaagtagac
    60001 aatatgctga aaattacgaa cgaagcgaag ttaatggcac tagatccacg cctgttagat
    60061 gactacgatg aatcaaaata cgatcgacaa gaattgaaac agaccaaaat tgcacgtgca
    60121 gctgaaaagg tattttccat ttggttagag acaaaagaac aacgatcgac acaacttatt
    60181 ttctctgatt ccggaacgcc aaaacctggg aaattcaatg tttacgatga aataaagcgt
    60241 cagctgattg aaaaaaatat acctgaaaac gaagtggcgt ttattcatga tgcaaaaacc
    60301 gaagcgcaac gtgatgcttt atttgagaaa gtccgagaag gtgaagtccg tattctgatt
    60361 ggaagtaccg gaaaagttgg gaccggtacg aatattcagg acaaattgat tgcagcacac
    60421 catgtagact gcccttggcg tccttcagat attgagcaaa gagatggccg gattgttcga
    60481 caagggaatg agaataaaga ggtccagatc tatcgttatg tggccaaagg aacatttgac
    60541 agtttcttat ggcagattca ggaacaaaag ctcacgtata tctctcaagt catgactggt
    60601 aaggcaatca ctcgaaccgt tgatgagatg tctgaaacag ttttagacgc ttctgagatc
    60661 aaagcagttg cgacaggtaa tccgctgcta gcagacaaaa tgcggttgga taatgagatc
    60721 acgaaattaa gaatgttaca atcttcgtat ttgaatgagc gagagctatt gaaacgttca
    60781 atcgatcaaa tctatccgtc aatgatcaaa agcggggagc agcatatggt cacgctacaa
    60841 gaagacattc aacgctataa agacaatgat cctgacgagt tttcgatcgt gatcgatggc
    60901 aaagaataca cagaaagatc aaaagcagga gaagtctttg aaagtgccac gttcctttat
    60961 atgcgctctc cagatttcgt ggaaatcggt gaatacaagg ggttttcgtt ttctgtgcga
    61021 aaaagcgaat taagctatgg aagtttgcaa gtacgtctac atggtaaagg gcattacgat
    61081 ttactcgtag atcctagcac ccaaattgga agcattcgcc ggatagagaa caaaatcaat
    61141 caactgcccg aaatgttaca agaagcagct gaagaagtcc aagaaacaaa agaaaagatg
    61201 gaaagcgcta aagaacaaat caaacaacct ttcagccacg ctcaagaatt acaagagaag
    61261 gtattgcgcc aacaagagat tcaatcagaa attgagtcct ctctaaataa gtcagacaca
    61321 caatcaatag ctgatgagat agaagaaacc gagaagtctc aagtagcgta ttccaaagaa
    61381 gattatttaa acgaagaaag ggagatgtag tttataaacg cacgctgaat ttaatcaata
    61441 gcaaactaaa aaagatcctc aatgaacaat ttgttttgtt atgaggatct tttcttttca
    61501 tgactcatga ttgccttgat tttctcgttc tttcaaccat tcttcaacta actgtaagtc
    61561 ttcttccgtg gccaagttta aaatgaaact tttggttgca gactttttat tcctatattg
    61621 cttcgctttg gcattttttt gttcatacct ttttacagcc tgtcttaatg cttcactggt
    61681 tttgtgcgat gaattatcca aatttatccc ccttttctat tttacactat atcaaaaaaa
    61741 tataagaata tccatataat gcttgcaaat ataagcatat ccttatacaa tagagttgta
    61801 aggaaataga ttttctaaat tagaggagga acccgttatg aacatgatgc taaaaaacaa
    61861 agaataccaa gtaagatttg aagaatatgc cgataacaac aatgtcgcaa tcatggtagt
    61921 aaaaccgaaa aaaggtagga tgcgtgaaga agaattagtc actactgcga ccgtaaacac
    61981 agatacagcg atcaaaccag attttgtagc aattaaaggt tggtctgaaa atacagggat
    62041 tgaataagct ttgattgatg cagatgtcat ttgcccagaa agacttggag cgatccgatg
    62101 tggtatggct gtcgcttatg tttatgaact aactcctaca gcaaaagaag ccaaaaagaa
    62161 tcaacagtag agcatggttt atggcacagg aaaacgattt atcctgtgcc atatctctac
    62221 attcactatg taagatcaat aaggagaatg catatggtaa tcgcaagagg gaacaaaaga
    62281 ctgccagtaa cacttaatgt acataggcaa aaaagtctat cacagctgaa aaagaagtat
    62341 agcaagagtg aatcaaaact tttgtgtatt gcgttagata tgttggttga gcaagaaaaa
    62401 gctggttttg aaataccgaa actgagagag tgataccact ttattcctta attcataata
    62461 gtgatggcac ttttttaaat aaaacagcag cttgattaaa tttaatcaac actacttaac
    62521 gcttaggagg gaaatcattt gatcacagca aaaagttatt tcagcggagc cggcggtatg
    62581 gatctcggaa tggtagaagc tggtattaat attttagagt cttttgaaat agataaaaaa
    62641 gcagtcgcaa cattaaaaat gaatttcgac catacaataa cgcaatgcga tactacccag
    62701 ataactgttt tagatcaaca ggacgctgat gtgtatattg ctacctatcc ttgtaccaag
    62761 tattcaaata tcgctgatat aagtggtaca cgtacaggtg atgatttgtt tcttcatttt
    62821 tttagacata tcgctttgtc aaagcccgaa gcttatgtga ttgaaaacgt accaggtatg
    62881 aggaaattcc aagttgtaat ggagtgtatg acaaagcttc cagattacta tgtaagggtc
    62941 gaatgtccag taaatgcgaa ctggtggcta cctcaagaga ggaaaagatt gattgtgatc
    63001 ggtactagaa aaccatttaa caacttggat tatccgcttg ctattgatca aacccctata
    63061 acagttgggg atattatgga aagtgatcca gaaattcata tacccgaata tgtgtacaaa
    63121 cgtcttaatg gtggctaccg agataaacca attattacac ctagaaacgg taaagcaccg
    63181 acatgtgttg cgcattatgc gaaagaccgt agcacacgtc tcgtagatga tggcaagcgt
    63241 ataagacctt acagcgtttt ggaatatcaa aggctccaag gttttcccga ctggttcaaa
    63301 tttgaaggta ctgataatga tgcatataga caaattggaa atgctgttcc agttgatatg
    63361 ggaagatgga ttgggcaaca gctgaaaaaa tactttgaat cttaagacaa gaaatgatag
    63421 aaaatcactt tagaaaggaa gatgaaaata tgggacatag tcagttagaa cataaaagaa
    63481 tatggcttga aaagttggaa aaagacctgt cagaactcaa atctttggga tatgaaaaaa
    63541 actccgaaac gtataaagag gctaaatggc aaactaataa agctcgaaaa aacctgaata
    63601 ggtgacatat gtggaaatag tcaaaatgag aaggtgcagg ggaatggaaa cattgaaaaa
    63661 agcactggaa aagagaaaaa ttgaatgtag ggttgaaact cccgacacgg tttattgcgg
    63721 atcaccgaag gatttgttac tggcaatatc ggaaaatgaa ttacagttgg tagtgattaa
    63781 taaacggctt gaatctttat ttggtgataa gcgttgggat attgaagtcg gttaatagaa
    63841 gaaattgcaa aatggaaagg aagataattt ttgagtaaat atgaaactaa agagtctaaa
    63901 aaactaaaga aagagttaag agagattgat gtaaaagggg ttgtaacgca tatagtattc
    63961 tcattaatag tttgtatgtt tcttggctca ttttattatt tttttagatg gaaaatcatt
    64021 ggtgcattac tattattctt tactgggtat ggaattttag gaatagtatt agttgctatt
    64081 tcgttatttg ttcagaaatg gggattaaag aaaataaaag aaaaagaaga aagtgactat
    64141 tttaaaaatg atgatttaga tttttgaaga agtcagctat tcactaggat agttgattag
    64201 tggaagaaat aacggaattg gagggttgga aatggagtta attagtgcag aaggtaaaga
    64261 acgaattaaa tcggtttaca gagacgtatg tatcgcagaa aatgcatata ggaatgatgt
    64321 tgggggtcac aatgggaatt ttgaacaacg taaaattgca attgatgacg tctttcaatc
    64381 tttaggcatg atacatgaag ctcgatatat agaggaagaa atctctaatc agacttggac
    64441 taggagtcgc catatcgatg ggcggattaa gtatgggtta gtccagtagt gacagagatt
    64501 aacaacttga ataacaattt tttgagaaaa caaaaaggca actcaagagt taccttttgt
    64561 aggtatattc tatttatttt ttcttcctat caatgccgta agtagcgaaa gcaggaataa
    64621 agaggaccgc taagaataca ccaacccatt taaatacttt aggaattttt ttcaaagaaa
    64681 ccgtctcctt tatagatatg attatagatt atagaggaga aaaatagtcc gatatgacaa
    64741 aaatgataga aaaatttatt aattgagcag tcagcaatcc accggattca aagaaaaact
    64801 gccctcttag tttccgctaa gagagcagca aattattatg tcttgtagag tatacatggt
    64861 tataagatct ttaacaataa gaaggtgtta tgacggtaag atttgtcgtt gacttttcgt
    64921 actatgagtt aatcataaaa cttaatacaa aaaatcagat tatccgcata aatacagtat
    64981 ttaaggacta ttcatcaatc aaaaaagcaa ttattttacc acgaatttac catgattgaa
    65041 tgagccttga tatgacagca aaagaacaag ggagattgca caaaagcgtg tacctccctt
    65101 ttcttattat ttgtatgaac ctttatttct acattttttt ctcaagcaca ctactgataa
    65161 tgattaaaat aatcggctgg attgcaatga tacttccgac aataatagct ggaatgatgt
    65221 agtctaaaca aataaatata actgttgcca cccctaacag tattgtgtta aacatatttg
    65281 ttatatttcc cgctttttct ttgattatga tattccgttc gtccataaca ttgattttgt
    65341 agtctgcatc attttttatc attttatcat gcaaaatttg aacatgagaa acgccaaaaa
    65401 cggtactgaa taaaatagca agtccaatat ctaccatttt aggaaaatct gtaaccaaaa
    65461 ttatcacaat caaaaacaac gaaacaatat aaccgatata ccaaatctga cccttatttt
    65521 tcatcttcac ttttctcctc atagataaaa atttcttcaa tgctcatttg aaagtacctt
    65581 gcaattttaa atgccaatat aatagaggga ttataacgtc cattttctaa agaacctatt
    65641 gtttgacgag atacttcaag agcagaagct aaatcttctt gtttaattcc tctttgtttt
    65701 cgtaattctt ccaaacgatt tttcacaaca tgctcctttc taaaaatgga aagttcactt
    65761 tcctttcatg cttttataat actcctgcga gcgcaaaatg tcaagtaagc tttccatata
    65821 attaatatat tcaatatcct gggtgaaagc caaaacgaag gaaaatgcaa tcgataagtt
    65881 aattagcaaa tatgactata ctcatattca gaatattaga gaattactta attaaacaag
    65941 atagcatata gagaaatatc ggaattcaaa aatggatttt tgtcacttta aaaaaattag
    66001 aaaacgctat cttattaatg gtaaactaaa ttaggaaaaa ggaattatcg tgatggatga
    66061 aaaagcaaaa caaaaaatag taaaattact tgctttagca aatgatgcta acgatgaaga
    66121 atcgatgtct gctttagcaa aagcccaaga attgatgctt cagtacaata ttgacgaatc
    66181 ggaagtattt agttacaaga aagagcaaaa agctaaatca gtgatcgatg tagttattta
    66241 tcaaggacgt ccgcaaaaat agttttaccg attggcgtca ataattgcta agaattttcg
    66301 ggttcgctac tattatgtat ccggcgcacc gattgaactc cattttgttg gaatcgaatc
    66361 agatgttcaa gttgcacaga tcacatttga atacgcccgt ggaagtgcca gcttctgtgc
    66421 aagacagttt ttgcaattac cgggaattcg tcgcaagtac aagcggaaat ggcagctaaa
    66481 gcaagattat cttgaagggt atttgcgggg attatcggag actttcagaa aacaggtgct
    66541 aacaaacggt tacgcacttg ttttacaagt gccagaagtg gtccaggctg cagtcaaaga
    66601 aattggactt gttcctagga aagacatgtc gcataacgtg aaagatcggg aagcatttta
    66661 ctctggatat gaagagggag tacgctttac tgataaaggt aaattaagtg tttaggatct
    66721 caatgaaagg agcggttgac ttgtcacaag aaagaaaatg ttacggtggt caatggaaag
    66781 taccgatcac actttacaac aaacgcttat attggccacc aagttggatt gaatgtaact
    66841 gtggtgaact cgcaaaacaa gtaagagaaa gaaagggaga tcatttgtat gcgaatgggc
    66901 gttacttatg taactcatgt caccgagaat atgagatggt ctatgggaaa aatcagttta
    66961 ttttggtgaa tgagaatgaa gcctgaaggg agtctcattt cttttgagaa taagcgaaag
    67021 aaacaatgga cagtcattgt cttgagtcgc ttttcaaaag agcttattgg atttttgagt
    67081 gtgactgctg ctactgaaaa tttatgtttt actcaaaaga aggaagagtt gggactattt
    67141 acagaagctg aagcaaatga aattcttgac tcaattaatg cgttgaaaga tgaagaagat
    67201 gtgatctaca aaacaaatga aaaaaccggc actgcagact ggatatccgg aaagtatttt
    67261 gattttaaga aagcaaaagt tcaatttgaa taaatcgagt ttaacaaaaa gaccaaggag
    67321 gtatagaaga attgactgat catgaaaaaa tagcaaagct tctgaataag aaatttagtg
    67381 caatggattt tacgaaaatt gaacgccttg aagatggtat ctggcatttg gtagataaaa
    67441 cggaagaaat gtttgttcat tttgaaggaa atgtagcaaa aattcgttcg atcgtaaact
    67501 cgattgaata tgaaaaatcg atagagttat aggataatga atttgtgata ggcttgtatt
    67561 taaagaattt ataggtagta gtagaataat atgtatatga tataataaat tatcgattaa
    67621 agttggtatg gagtgatgat acggtgtcta tttttaatta tatattatcg aatattacaa
    67681 aatggaacgg aacaacttct agagggaaat actggcaaat tttttttatt gtatttattt
    67741 ttggctttat tttagaagta atttcaagac agatgactgg tatatcgctg atccagcaaa
    67801 gtttttattt caaatataca agtttgttac actttatatt attgttattg tttactttgc
    67861 ttaatctaat gttgtgggta tttcaaatca gaagattaaa cgacttagat ttaactagat
    67921 ggctggtttt agtgaattta atacctatag ccggtcccac aataatgacc ttattaatgt
    67981 taaaaaaagg aaaaacactt taaatttata attgcttgaa tagtttgatt aaacgtgttc
    68041 tttttcttaa cttattgcta aaagtgatag tggacattct gagatcttct agttaatatt
    68101 ttcatgaaga aacaccataa ataacaaaaa ttgttattta tggtgttttt acatctgatt
    68161 tattatcagt attacttaca agacaaggaa ttgtaagctc cttgtatagc agcttggatt
    68221 gtaagccctt taaatgctac accacttaaa aatgctttag cgatacattg attatgtgat
    68281 ttttgtaaag agtaatactt tgagcgggct tcttttctaa tttttgcatt tggtatttgg
    68341 tttaaagcaa attctgccca tgttcctaat ccatcatctt gtgcataagc aacggtagta
    68401 cctaacggca aagttgaaga tattgtcgat aaagcaacac tagacaataa aagacacagc
    68461 aaagcaacgt ttatagtttt tttcaagtta aacgctcatt aaaaaatatt taattgctaa
    68521 attaattata aagtgtataa aaaataaatc aaataaagat tttatttttt gcaaaagaac
    68581 tttaaaaagg ggtcaaaaac taaaaaaaac ttaatttctt atatctaact atttttagga
    68641 tacttattat aattttgact atttggataa aaaaaacact tttaaaatga caaataaagc
    68701 gttatcattt aattagtttc atctattgtg ataaattgat caatgtttaa tacttaatca
    68761 ataaaaagga gagaaaatat atgaatatag ataatgaaaa acaagtattt gcttgctctg
    68821 ctgcaaaaaa atcgaataca ctacctactg ctcctatgaa tgtatttgct tgctctgccg
    68881 caaagaaatc caatacgtta cctactgaac cattaaagtc tgttagttgg ggtgaaaaac
    68941 aatagatgaa aatcgagtat taaactattg atcacaaaat gaggagataa tatgaagtga
    69001 ccccgctagg ttgtcaagtc agggatttaa acacataata attcatatga acaaaattat
    69061 taatgggtta ttaccctatg acaataggag gtcactatga aaaatataat atatatcggg
    69121 atggatgtcc ataaagaaac gtttaattta tgtgctttac acggcttaac aggtgagata
    69181 cttggagaaa caaaatgtgc ttccgatgtt tctttggtta tgaaatttgt aagaaagctg
    69241 aaaaaaagac acggagaaga cactgcgatt ctcgcaggct atgaagcagg ttgcttaggg
    69301 tactcattat acaatcagtt gaagagtcaa ggtgttgagt gtgatattct ggctccgacc
    69361 accatgtaca gttcgataaa aaataagatg gttaataatg atcgctctga tgccaaaatg
    69421 attgcttcaa acttggtgaa tggtacctac aaatccgtat acgtccctca agaagatcat
    69481 gtggaagtaa aagagtatat ccgaatggtc aatgatttta aaacatcttt gaaaaaaata
    69541 aagcaacaaa tcaaagcgtt ggttttaaga catggctaca aatatcctgg gaaatcaagt
    69601 tggactttag cgtatatgaa atggttgaaa tctttagact taaaaggggg ggtgaaagag
    69661 acgttggatg aatatctttt acagtacgac acattgatag ataagattca acgattttca
    69721 gaacgcttag aggaactatc ccatagggat agctacgaag aaccagtagc cgcattacgt
    69781 tgtttaaaag gcattgatac gacagcggca atgatggtgc atgtcgaaat ttcagatttc
    69841 actcgctttc caaccgcaaa agcatttaca gcttatttag gtttgacgcc aagtgaacaa
    69901 tccagtggtg aaaaaattag tagaagtacg atcacgaagc aaggaaattc gacggtaagg
    69961 tctactctag ttgaatgtgc caatgcatta gtgaaaggta ccatcgggat aaaatcaaaa
    70021 agagtcaaag caaggcaaaa aggacaggaa agtcaggtga tttattatgc ggatcaagcg
    70081 gttgaacgtc ttcagcgaaa atatcatcga atgatttacc aaggaaaacc tcggaatgta
    70141 gcaataactg cagtagcaag agaattagct tgtttcatct gggggataga aacacaaaaa
    70201 atgatgtaaa aaaaattaag aaaggtgtgg agtgacaaaa aaatagtagt tgacaaacaa
    70261 ttgatggcgt agaaaaactt cagagataga tgcaggtctg gctatctacg acctacctct
    70321 tctggcacag attcgttttg agggattagc tatccaaagg aaacggcata atctgtgatc
    70381 cacgcaggga gattatacga gcctaacgac ggaccattaa cctgaggtaa ccaatccacg
    70441 aataacagag tggttgactg tcgatagatt ttatttctgg agcttttgta cgtcatcaat
    70501 tcgttcattt ttgtgaacaa attgactatt gacaaaggtc acttcataac aggtagttta
    70561 tgattactta taacagatta gacaatcgaa aggttttgat ggatgacttt tctagagaat
    70621 atgtattgga ttcaaatatt tcagaattac gaacaaaaaa acaaatgcag tatttaaaaa
    70681 ctatgatgaa tataaagctt atacttgtca gatagattat ttggatactg tttactacaa
    70741 tgtgacttat ttatgtaatt tgtactgtcc gtattgttat gcgccgcgca atacattttt
    70801 tgtatcacag gaaaataatg tagctttctt aaaaaaatta gtagaattaa atgcaaaaaa
    70861 tatcgtttta attggtgggg aaccaatgat gcaccctaag ttagataaga taattgatga
    70921 gataatttct tatggattta catcaatatc tattataacc aatggtacta ttttaaaaga
    70981 aaaagtttta gaaaagattg tgaagtataa tatagatatt caaatatctg ttgatggtta
    71041 tactgaggaa acaaatgcac ctacacgtgg aaaagggagt ttaaataggg ttcttaaaaa
    71101 tatcgatata ttaaaacaga ataatgtaaa ttattctgta atgcaggttc ttactaaaga
    71161 aacaataaaa aattcagaag aattttctga atttttttcg aaaaagggta ttcctcatgg
    71221 attttttctg gtaaaagaaa ccgatgatac gagtagaccc tctttaaaaa gtatatataa
    71281 gttatatgat ttcttatatt ctcgttatgg agatgtgaac aaagtatttg actgtgttaa
    71341 atcatccgat caaatgcaat taagctctac tggatttcct attactcact gtggagctgg
    71401 aataactaca ttaagtattg atccattagg aaatgtatac ccttgtgtaa agctacataa
    71461 tgcagatttt aaaataacaa atttaactag atctactgca gttgttgaca taaaaaataa
    71521 tagaaatagg gttttagaaa atgaattagt agatagtgtt gataaatgca agaactgttc
    71581 tattaagtat atatgtggtg gaggttgtcg agcagaagaa tattatgtgt ctaagaacaa
    71641 acaaagttat tcgcaatgcg accttcaaaa agggaattta acttattttt tggagaatat
    71701 aaatgaataa aaaaattgta tcttttttat gtttaaatgt ccttgttcta ttttctacga
    71761 ccctgtttca attaaatata ctatgggaaa tttttagcca acaagaagaa ttaaaaaatt
    71821 tagtcatgat tattggttct tcatttattt tacaagatgg aatgtctctg gttgtgggaa
    71881 taattgtaga ttcttattcg aagaaaaaag tctttttttt cagtattttt ggatttataa
    71941 taactgttgc tgcaagctat tttattaata tggatattca atgggcagtg ttttacttaa
    72001 tttttactgc agtaaataca ttatttttaa gatgcttagt ttctgcagca gctgaaatgt
    72061 tgacaacaga tgaattcatg aaatacgacg gtattacagg attgtttaat caaatactta
    72121 tcattataag taatgtgatt tcaggtatat tgattaagtt tgtaagtgta gaggcaatct
    72181 attttactat tattttcttg ttaattttag ctttgtttct aatttttttc ttacccgtta
    72241 aagatataca aggaaatgag agtatcaagg acaatgagga aataaaagaa aagattgaag
    72301 gctccctgtt gttgtttatt aagaaaaata tttttaaaga taagaaaatt gtagtgttta
    72361 tttgtttgct tttcttatta aacttggatt atggatatat acctaatatc ttaccttatt
    72421 atatgtttaa gtatacgagt caaacttcac ctattttctt atcattgcta aaatcttctg
    72481 ttaatttagg agagatagta gcttcatttt ttgttatcca ttatagaaaa aaagtatcaa
    72541 aaatgacaaa aataggatta ctaggatcgg ctatctgttt tatgttactt cctttcagta
    72601 atcaaatgat tttagtgaat ttttttgttt tatcgttcta tggctttttt gatacactta
    72661 cacaaccatt gttcagttat tttgttagtt caattgagca taagaatcgt gggaaaattc
    72721 tgggtatcgt tgattgtgtt gtttatttgg ctgtcccaat tggaatgtca ttaggtaatt
    72781 ttattagtca atatggtatg ttgatactga gcctttcgat aagtatagta ttcttttgtt
    72841 cgtatatttt acttgtaaag acgaagacat ataaaagtat tgatttagca ggagatttgt
    72901 gatgaatctt attcaggaaa agtttttaca gaatgagtta gtttggttga aatctctttc
    72961 atgtgagatt cattcgactg aaaattatat cattgttaaa agtgattttt ctcaatctga
    73021 agatttcaat tttattttcc ctcgaaaggt agttcaagaa aaagacttat acatgataat
    73081 ggatcaatat aaaataaagt atctaaaaat agctacccct acattgattg atttaaaatt
    73141 aacttcagca atgacctgtt taaaaacgta tgatttgaat tttaatgcga atgcttcatt
    73201 atcaaatttt cgcaaacttt gtagtaagaa aaaaggaata aggctttcag caaatattcc
    73261 atggaaatac aatgatctac ctaatcgtat attttcatca aatgtagttc cattcaccat
    73321 ttgtttcaat caatatgaag ttggacaatt ttgtttagta attcttaaaa gcaatattgg
    73381 tatatatgat ttacagatat ataaagaatt tcaaaatcaa ggctttggaa atgaattttt
    73441 ggaggagcta cttcttgtta agaatgaatt agagattggg aatattttta ttcagacctg
    73501 gaatcagaat ataattgcaa aacaactata ttcttctaaa gggttcaatg taataggaaa
    73561 atatcaatat tatacttaca cagaagagtg ttaatactga ttgcattata ttgaaagagt
    73621 gttcagaaaa tgacccgttt ctgaacactc tctttttgtc ccaaaattat acagaaaatc
    73681 tcaaatttag tgttcagaaa cccgtaacaa aatctatgtt cagttattac ctctggatta
    73741 agttatggta caatggtttt aatcgaggag tgattttttt gaaaattgga tatgcacgag
    73801 tttcaactgg attgcaaaat ttggatttac aagaagatag tttaaaaaaa tttggctgtg
    73861 aaaaaatttt tactgatcat atgagtggag cgaaaaataa tcgaccagga ttagaaactg
    73921 cgattgattt tgtacgatct ggagatactc ttgttgtttg gcggttggat cgattgggac
    73981 gtaatatgga ggacctgatt tcaatcgtca atcgattgaa tgaatggagc gtgagtttcc
    74041 atagccttca ggaaaatatt acgatggata aatccagttc aactggccag ctgatgttcc
    74101 atttgtttgc ggcttttgca gaatttgagc gtaatctgat attagagcga tcggcagctg
    74161 gtagagaagc tgctcgagca agaggccgat atggtggtcg gccggaaaag tttacggatc
    74221 aggatctaaa gcttatgaag acattagttg atagtggaac tccaatcaaa acgattgccg
    74281 aacgttggca ggtctctcgg acgacaattt atcgctattt agaaaagatg gagaagacag
    74341 agtctgaaga ggaagaaatt atacaatagg aatactgcga ggggaaagtg atgaaagaaa
    74401 aggagattca tttagcgatt gtaaaattga ctgaggatcc agagtggata cattgtgccg
    74461 agattgtggc ttacgtgcaa gaattaagca aaaagttctc tactactcgt cagtcgccga
    74521 ttaattacaa ttctataaat gggatcaaag ttgtatactc aaatggttca cataggatct
    74581 atccttccct gaagcaatgt gctttagaag agaaactgta taatggcact attcaaagag
    74641 tcatacgaat gaaatcaaag acaagtgatg gccgacaatt tattgtgatt taactttttt
    74701 gtcttttatt ctttggtata attaatatag atctgcttaa catagacttg cgctgcccgt
    74761 tattgaagtt tcaggcagcg ctctttttag ggaattatat atggaagaaa aaacgatatt
    74821 tgagaagcgc tggcaattag catctagtga tcaacgtgca aggtttgaca aacttttatc
    74881 ctcctatcct acaattgaat ggacgtacaa ggagaaaaaa tatttgctat ggttatgtca
    74941 gttagatgtg gatacatttg aaactataga aatcattttt gaaaaaatca agtgaagcaa
    75001 caataaaatg caaatagata agtgacttga gagcaaatta aaagctttgt tagattatat
    75061 tcgagaaggc gatattgttg ttgtgacgga attagatcgt ttaggacgta ataacaacga
    75121 tcttacagcg attatgaatc atattcaaca aaaaggagcg actttagaag tgttgaattt
    75181 accttctatg acggggattg aagacgagaa tttaagaaga ttgataaata atctagtgct
    75241 tgaattgtat aagtatcaag tagaatctga aagaaaacgg atcaaagaac gccaggcaca
    75301 aggaatagcc attgcaaaac accagggaag gtttaagggg cgtaaaccca aatatacgtt
    75361 agaagatgaa tgcctgcaac atgcttttgc tctttatgat caaggattaa cagaagcagc
    75421 aattgcacga agaactggca ttaatcgaac aactttttga aggtaccgag aaaaacggga
    75481 ggaataaagc gattagttta tttggtaatt ttggtagatt tcgtgaaatt catttgttag
    75541 ctgtttaagc aattgttgtt gtaaaaagca attgttttga atttttagtt caggatagcc
    75601 ttcaatggtt tgattttgtt cgttgtaatg tgtgattgcg cgatagtgtt cccgttctag
    75661 aatataaaaa gtatcatgac cgaattgttc gaatgtacaa aggtgtttct gttttagttg
    75721 atcggtgtag atattcgcaa agtgttctga agacataaaa ttcttttcat gggaagcgaa
    75781 aagatgttct tttttgagga tattttgtgt caaacattga gacaagtaac gataatctgg
    75841 gtgtctttgg taagttgctt caaattgttc ataaagcaaa gtgatttttt gttgttcggc
    75901 ttttaggtat tggcgttctt tagaatagaa ggtatcaata gcatacattt ttttattgcg
    75961 ccttcgaata attccatgta gtgttgcaca aatttgcctt tttgaatggg ttccccaaac
    76021 ggggtaagat ttgtaaacat gaagtgttct cctttctctc aagggaagag gttttccctt
    76081 gagtatgtga tagtgaatga tttaagaaag catgggaact ggttcaattg gtctttctcc
    76141 tgtttgtttg gcaataagca acggtaagat ctgttgtagc cattgttgat tagggacttg
    76201 atctaaaaag ttcccgatgg tggttaaaat gagtgtatct gttcgctgtt ccactaaata
    76261 aacggattga aaaggacttc gtccagcaat ataattgatg atgttttcat ctgtttgttc
    76321 aaacgagcct aaatagtcat tggtattccg gtcgtaacaa tcaacaatag gaatggaatt
    76381 tgtgtttgtc attgcaatta ctcctttgct tttttatttt gaatcaagag ggaaaagatc
    76441 cctcttagta gtaactactt tcatatacag gttgtccgtc atatcggtaa aaggtatccc
    76501 aactacgatt gacatagagt tcatttggat gatttagatt gattcgtttt ctaacaatgc
    76561 tgtcttttgt tcttgcaagt ggataatcga taaatttatt tggtatgggc gcagtcgttc
    76621 cactcattga ggtatgaatg tctatcgttt gtttcttaac aggtctgaac caggcgtttt
    76681 tttccgttaa tttcacgatt tgccagaaag taacattggt ttgttcatag ccccaagagc
    76741 tataaaagat gtctccgact tggtacgggg gtttttgttt gtgtgatcat aggtgattcc
    76801 tcctaaaatt aaaaattttt tgttttcttt gagagttgcg cctccccctc ctaccttttc
    76861 ggtggagtaa gttggctggg gtgttagggg caaagcccct aaggtcttga tttgagtgac
    76921 ttccatagtt agctgtaaaa tcaagaaaaa aagattctgt tgtcaaagat caaaagtgaa
    76981 aattcagagt agattttctt ttcgtgcgtc ggtcgtttgg cataaccggt tcgaaaccat
    77041 gttctgactg ctgaaaaggg gcagcgaaaa aatgaaaaaa atcgtgggtt tctatttttt
    77101 acatttttgg ttcaaaattt tcttaaaatt ttgacgctga tacattttac ccttggaagc
    77161 aggcaggtta tggtttaggt tatgctagcc aaatgactga ggcatgaatt agaaaatctt
    77221 ctaaggtttc gcttctttga caactgaatc tcttgatccg attcatagct ggaaaagtca
    77281 ctcaaattca gacgcttagg cgtctgaaac acgagacaag caagttttac agaaggaaaa
    77341 gaggaagaag atcacttaag aggggagact agaagtagaa aaaagaagaa ggggtgaaaa
    77401 aagatgggaa taacaagtaa tcgccagtgc tacaacagct attacgtgcc gtttgaaaca
    77461 ctgtattatg caagctggcc gcaacctatg taagatgcga ttgtggggaa ttggcccaac
    77521 atatcgtaca ttttaagctg ctaccttgtg gagcgccaca ttttcaaaag acgtttattg
    77581 gaaaatgtga acattgtgag gcacggtatc gacaaataaa aggcacattt ggttttgagc
    77641 gaataaacga aggaaaggtg tatcccaatg agtaaagaag aacgaccggt agagtgttac
    77701 attctcggcc agcgtaacga gcacgcctaa ttgaatggtt gattgatgat gtagtacgag
    77761 actggcgtac gttggatgct tacttggaac aaaccggtta tctttgtgag tatgcagatg
    77821 gggagtgctg gaaatatcgg ttgagaacgt ctaatcaaat tgacttttat attcggaaaa
    77881 tcgtggttcc agcggacgac aaaaaaacga ctactttttc taagtagtcg ctacgttgac
    77941 aaaggtagat ttgtgagatt tgcataattt ggatttgtca acgtacaaaa agaatatcaa
    78001 aaaataaaat agaagaaaag cggaaatcca ttcgatttct gcttcttggc atttttgaga
    78061 ataatttggt gaaagaatct aagaaggtga aagaaatgcg gcggttagaa gatgaaaaaa
    78121 agaaggatcg ggaaaaattg agttgttttg ttatttgtgt cagaagcgcc ccaatctatt
    78181 tatttttgaa ttatctgaat taattgaggc cccaatcgag aaaacatttc tatggatcaa
    78241 ggggtatcat ttaccttatt attggaagta tcaacgttgg agggaatgaa atggatgtta
    78301 agagaagaaa aaatcaggtg tatatggatg ttttagaaaa gaaaaagggt agtcaaatag
    78361 atgaaaatat ggaaaaatag gaacaaaaaa gagaagggat acgtatgaaa aaagttttta
    78421 ttgttcttat tgccggattg acgatcatag ggagtggttt gtttatagga aatctctttt
    78481 tttcaaaaga ggaagagaag gaaccttcta ccatagctac tacagaagaa ctaaagcctg
    78541 aactgtttct agacattgaa attttagagt caaatcaaga agaaagccaa caagaaacag
    78601 ccatcaacga actctatcga ggggaactgg atttccaaca tgccgatcca acaaaaccgt
    78661 atctcattac tgtacagttt atagataaag aaaaacggga attttttgga ccagataaac
    78721 aacctttaag actggaaaaa gaagtcactt tagggaaaga acaaggaacc gtttttcttg
    78781 tagtgaaggg aaataaaccg atgttggcac atatccagaa attaaaagat gcacaagttt
    78841 ctgcatcatt aaaagaaaag gagtaggaga agaggagagc ttgtgatgga cgatgaaatg
    78901 aaccgaagtg tttattctta caaaattctg attagtaaag aggcagtaag agaaaagttt
    78961 gatgtgtatt cgttgaagaa tcatattatt tttcagttac atggctatac gtataactcg
    79021 tatgatcgga tcaactatac gatcaatctt tctctaagag aaatggtagt aacgatgact
    79081 aaaaaagacg gaagtacatt ttccgcaaat gaatggagat tttttgatcg aatccttccg
    79141 gaattttttg agaagttaga agtagcgttt atacaggaaa aagaaataat ggaagaatga
    79201 atagggatcg aagcagaagc ttcggtcttt tttattatta acattaaaaa tagagtatac
    79261 ttaaacgtta aaaaacagaa ccttgataaa tcaagcttct gtttgtaaat tataagtgtc
    79321 aattaattgg agtgaaaaat tatgcatatt ggatatattc gtgtattttc aaattttggc
    79381 tatggtagcg gaactcgaac gttaggaaat caagcaaaga caagcacaag gaatcgcatt
    79441 agcgtatgaa aaaggactgt ttagagggag aaagccggat tgttcactaa caagtagaaa
    79501 tcggcaaaaa caaattattt attatcaaat cgtcgaaatg ttaaaacaag gaatggggat
    79561 ttcggaaatt tctagaagag caggtgtctg tcgacaactg tttataggat caaagaaaat
    79621 ttagagagaa acgagatcca tgtggagtaa gcgccacctg gcttatttgt ttttatctat
    79681 aatttttaaa taacgaatat aattaaatag ctttaaaatg aaaataaatt aaatgttaca
    79741 atcaatttaa ttatacagta aaaaaataaa aataaaagac tgtttttaaa aggattttta
    79801 tggaatactt cagttgtaaa aactatcaca aggataggaa ggaggcaaat tagtgagaac
    79861 aattattctt gttgatagta aaagataaca aaaaataaaa tataggaagt gggcgatagt
    79921 atgaatttac gagtatcaca atgttcaaag tttctttatt tggtaattgg attagtagtg
    79981 acagtaagca tattattcgg agtttctata aaaggacatg ctgatgaaaa gacacctgat
    80041 gttgaaaatt cagtttatga tatggatgaa aatcaaattt taaaaattat gaatgaatat
    80101 atatattaat gagaatggta tacgagaatt cgatttaaat agagctattg ctaataattt
    80161 gcctgatgaa atattagaga ttggtaacac gatgaataat attgtcttag aagtagaaaa
    80221 agaaaagaaa aatgataact taactgtaag aatcgttaaa agagcaacat ttttagttta
    80281 tggtaattat tgtgggtatg gaaataaagg taaaggaaaa atgcctatcg atgatctaga
    80341 tactgcttgt atgtaccatg attattgtta tgttcatggt gggaataaca cagattgtaa
    80401 ccggaaattt gcagatagat tgcgtgcagt tataaaaagc gcaaaaaaat tctcatacaa
    80461 atataatgtt gcggtaggcg cacttgctat ttttgggtga ataatatgat gaaaaaatat
    80521 ttaatatcta taaatatttt tttgttgctt ttttctgccg ctctaataat ttgccagcta
    80581 tttttttacc aagctaaagg tattccaccg attatgttta ctcctgtaac tggtatttta
    80641 ggaataatca tggctttgtt tttacccact aataatcatc tgagaaagat atttctcatt
    80701 gttaatagta gtattactct tattgttttt atttattttt tagccgtggc tataaatcca
    80761 gatttctttg tatagg
//
LOCUS       DAZABD010000005        71410 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000005 DAZABD010000000
VERSION     DAZABD010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 71410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 71410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..71410
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.005"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..205
                     /locus_tag="ACHALG_000358"
     CDS             <1..205
                     /locus_tag="ACHALG_000358"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2585799.1"
                     /translation="PPDFLIAQVYLEVHLLVRYCSMGRLLNTEKMVFEHHLFKIKKDC
                     DTNFVTTFKCIPRAKKLSEEIQF"
     gene            complement(239..523)
                     /locus_tag="ACHALG_000359"
     CDS             complement(239..523)
                     /locus_tag="ACHALG_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2585800.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(541..1431)
                     /locus_tag="ACHALG_000360"
     CDS             complement(541..1431)
                     /locus_tag="ACHALG_000360"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFN2585801.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(1451..1669)
                     /locus_tag="ACHALG_000361"
     CDS             complement(1451..1669)
                     /locus_tag="ACHALG_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HFN2585802.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(1639..3339)
                     /locus_tag="ACHALG_000362"
     CDS             complement(1639..3339)
                     /locus_tag="ACHALG_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFN2585803.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            3606..4379
                     /locus_tag="ACHALG_000363"
     CDS             3606..4379
                     /locus_tag="ACHALG_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2585804.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(4460..5029)
                     /gene="thiT"
                     /locus_tag="ACHALG_000364"
     CDS             complement(4460..5029)
                     /gene="thiT"
                     /locus_tag="ACHALG_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HFN2585805.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(5095..5197)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(5203..6174)
                     /locus_tag="ACHALG_000365"
     CDS             complement(5203..6174)
                     /locus_tag="ACHALG_000365"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFN2585806.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(6268..7641)
                     /gene="glmU"
                     /locus_tag="ACHALG_000366"
     CDS             complement(6268..7641)
                     /gene="glmU"
                     /locus_tag="ACHALG_000366"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFN2585807.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(8144..8962)
                     /gene="purR"
                     /locus_tag="ACHALG_000367"
     CDS             complement(8144..8962)
                     /gene="purR"
                     /locus_tag="ACHALG_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HFN2585808.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(9129..9953)
                     /locus_tag="ACHALG_000368"
     CDS             complement(9129..9953)
                     /locus_tag="ACHALG_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFN2585809.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(9926..10606)
                     /locus_tag="ACHALG_000369"
     CDS             complement(9926..10606)
                     /locus_tag="ACHALG_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFN2585810.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(10629..11567)
                     /locus_tag="ACHALG_000370"
     CDS             complement(10629..11567)
                     /locus_tag="ACHALG_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFN2585811.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(11812..12663)
                     /gene="ispE"
                     /locus_tag="ACHALG_000371"
     CDS             complement(11812..12663)
                     /gene="ispE"
                     /locus_tag="ACHALG_000371"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFN2585812.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(12817..15390)
                     /gene="mprF"
                     /locus_tag="ACHALG_000372"
     CDS             complement(12817..15390)
                     /gene="mprF"
                     /locus_tag="ACHALG_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFN2585813.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLP
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            15531..16337
                     /gene="proC"
                     /locus_tag="ACHALG_000373"
     CDS             15531..16337
                     /gene="proC"
                     /locus_tag="ACHALG_000373"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFN2585814.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(16416..17972)
                     /gene="rny"
                     /locus_tag="ACHALG_000374"
     CDS             complement(16416..17972)
                     /gene="rny"
                     /locus_tag="ACHALG_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HFN2585815.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(18383..19432)
                     /gene="recA"
                     /locus_tag="ACHALG_000375"
     CDS             complement(18383..19432)
                     /gene="recA"
                     /locus_tag="ACHALG_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFN2585816.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(19541..20782)
                     /locus_tag="ACHALG_000376"
     CDS             complement(19541..20782)
                     /locus_tag="ACHALG_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFN2585817.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(21022..21600)
                     /gene="pgsA"
                     /locus_tag="ACHALG_000377"
     CDS             complement(21022..21600)
                     /gene="pgsA"
                     /locus_tag="ACHALG_000377"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFN2585818.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(21809..22732)
                     /locus_tag="ACHALG_000378"
     CDS             complement(21809..22732)
                     /locus_tag="ACHALG_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HFN2585819.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(23471..24757)
                     /locus_tag="ACHALG_000379"
     CDS             complement(23471..24757)
                     /locus_tag="ACHALG_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFN2585820.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(24750..26018)
                     /locus_tag="ACHALG_000380"
     CDS             complement(24750..26018)
                     /locus_tag="ACHALG_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFN2585821.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            26270..26731
                     /gene="mscL"
                     /locus_tag="ACHALG_000381"
     CDS             26270..26731
                     /gene="mscL"
                     /locus_tag="ACHALG_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFN2585822.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(26790..27236)
                     /locus_tag="ACHALG_000382"
     CDS             complement(26790..27236)
                     /locus_tag="ACHALG_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN2585823.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(27329..27574)
                     /locus_tag="ACHALG_000383"
     CDS             complement(27329..27574)
                     /locus_tag="ACHALG_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HFN2585824.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(27689..28267)
                     /locus_tag="ACHALG_000384"
     CDS             complement(27689..28267)
                     /locus_tag="ACHALG_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFN2585825.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(28344..28874)
                     /locus_tag="ACHALG_000385"
     CDS             complement(28344..28874)
                     /locus_tag="ACHALG_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFN2585826.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(28878..29738)
                     /gene="rlmB"
                     /locus_tag="ACHALG_000386"
     CDS             complement(28878..29738)
                     /gene="rlmB"
                     /locus_tag="ACHALG_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFN2585827.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(29731..30126)
                     /locus_tag="ACHALG_000387"
     CDS             complement(29731..30126)
                     /locus_tag="ACHALG_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HFN2585828.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(30123..31535)
                     /gene="cysS"
                     /locus_tag="ACHALG_000388"
     CDS             complement(30123..31535)
                     /gene="cysS"
                     /locus_tag="ACHALG_000388"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFN2585829.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(31532..32068)
                     /gene="epsC"
                     /locus_tag="ACHALG_000389"
     CDS             complement(31532..32068)
                     /gene="epsC"
                     /locus_tag="ACHALG_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HFN2585830.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(32154..32393)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(32390..33850)
                     /gene="gltX"
                     /locus_tag="ACHALG_000390"
     CDS             complement(32390..33850)
                     /gene="gltX"
                     /locus_tag="ACHALG_000390"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFN2585831.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(34020..35177)
                     /locus_tag="ACHALG_000391"
     CDS             complement(34020..35177)
                     /locus_tag="ACHALG_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HFN2585832.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(35211..36581)
                     /gene="radA"
                     /locus_tag="ACHALG_000392"
     CDS             complement(35211..36581)
                     /gene="radA"
                     /locus_tag="ACHALG_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFN2585833.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(36644..37171)
                     /locus_tag="ACHALG_000393"
     CDS             complement(36644..37171)
                     /locus_tag="ACHALG_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFN2585834.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            37401..38207
                     /gene="proB"
                     /locus_tag="ACHALG_000394"
     CDS             37401..38207
                     /gene="proB"
                     /locus_tag="ACHALG_000394"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFN2585835.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            38204..39451
                     /locus_tag="ACHALG_000395"
     CDS             38204..39451
                     /locus_tag="ACHALG_000395"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFN2585836.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            39527..39871
                     /locus_tag="ACHALG_000396"
     CDS             39527..39871
                     /locus_tag="ACHALG_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2585837.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(39988..40314)
                     /locus_tag="ACHALG_000397"
     CDS             complement(39988..40314)
                     /locus_tag="ACHALG_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2585838.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(40687..41888)
                     /locus_tag="ACHALG_000398"
                     /pseudo
     CDS             complement(40687..41888)
                     /locus_tag="ACHALG_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            42005..42520
                     /locus_tag="ACHALG_000399"
     CDS             42005..42520
                     /locus_tag="ACHALG_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFN2585839.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(42719..43165)
                     /locus_tag="ACHALG_000400"
     CDS             complement(42719..43165)
                     /locus_tag="ACHALG_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFN2585840.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(43212..44690)
                     /gene="msr(C)"
                     /locus_tag="ACHALG_000401"
     CDS             complement(43212..44690)
                     /gene="msr(C)"
                     /locus_tag="ACHALG_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFN2585841.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(45044..45673)
                     /locus_tag="ACHALG_000402"
     CDS             complement(45044..45673)
                     /locus_tag="ACHALG_000402"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFN2585842.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(45657..46121)
                     /locus_tag="ACHALG_000403"
     CDS             complement(45657..46121)
                     /locus_tag="ACHALG_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFN2585843.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVCVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(46608..47876)
                     /locus_tag="ACHALG_000404"
     CDS             complement(46608..47876)
                     /locus_tag="ACHALG_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFN2585844.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(48107..48844)
                     /locus_tag="ACHALG_000405"
     CDS             complement(48107..48844)
                     /locus_tag="ACHALG_000405"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFN2585845.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(48911..49480)
                     /locus_tag="ACHALG_000406"
     CDS             complement(48911..49480)
                     /locus_tag="ACHALG_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFN2585846.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            49608..50480
                     /locus_tag="ACHALG_000407"
     CDS             49608..50480
                     /locus_tag="ACHALG_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFN2585847.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            50822..51694
                     /locus_tag="ACHALG_000408"
     CDS             50822..51694
                     /locus_tag="ACHALG_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFN2585848.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            51691..53649
                     /gene="nagE"
                     /locus_tag="ACHALG_000409"
     CDS             51691..53649
                     /gene="nagE"
                     /locus_tag="ACHALG_000409"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFN2585849.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(53900..54751)
                     /locus_tag="ACHALG_000410"
     CDS             complement(53900..54751)
                     /locus_tag="ACHALG_000410"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFN2585850.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            54874..55341
                     /locus_tag="ACHALG_000411"
     CDS             54874..55341
                     /locus_tag="ACHALG_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFN2585851.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(55604..56086)
                     /locus_tag="ACHALG_000412"
     CDS             complement(55604..56086)
                     /locus_tag="ACHALG_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFN2585852.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(56186..57049)
                     /locus_tag="ACHALG_000413"
     CDS             complement(56186..57049)
                     /locus_tag="ACHALG_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFN2585853.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            57647..58495
                     /locus_tag="ACHALG_000414"
     CDS             57647..58495
                     /locus_tag="ACHALG_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFN2585854.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            complement(58666..59007)
                     /locus_tag="ACHALG_000415"
     CDS             complement(58666..59007)
                     /locus_tag="ACHALG_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HFN2585855.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SMEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAAEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            59093..59800
                     /locus_tag="ACHALG_000416"
     CDS             59093..59800
                     /locus_tag="ACHALG_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFN2585856.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            59787..60110
                     /locus_tag="ACHALG_000417"
     CDS             59787..60110
                     /locus_tag="ACHALG_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFN2585857.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(60199..61236)
                     /locus_tag="ACHALG_000418"
     CDS             complement(60199..61236)
                     /locus_tag="ACHALG_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFN2585858.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(61683..62666)
                     /locus_tag="ACHALG_000419"
     CDS             complement(61683..62666)
                     /locus_tag="ACHALG_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HFN2585859.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
     gene            complement(63082..63852)
                     /locus_tag="ACHALG_000420"
     CDS             complement(63082..63852)
                     /locus_tag="ACHALG_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN2585860.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(63845..64780)
                     /locus_tag="ACHALG_000421"
     CDS             complement(63845..64780)
                     /locus_tag="ACHALG_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN2585861.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(65374..67023)
                     /locus_tag="ACHALG_000422"
     CDS             complement(65374..67023)
                     /locus_tag="ACHALG_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFN2585862.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            complement(67218..68114)
                     /locus_tag="ACHALG_000423"
     CDS             complement(67218..68114)
                     /locus_tag="ACHALG_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFN2585863.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(68107..68787)
                     /locus_tag="ACHALG_000424"
     CDS             complement(68107..68787)
                     /locus_tag="ACHALG_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFN2585864.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(68788..69570)
                     /locus_tag="ACHALG_000425"
     CDS             complement(68788..69570)
                     /locus_tag="ACHALG_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFN2585865.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(69563..70480)
                     /locus_tag="ACHALG_000426"
     CDS             complement(69563..70480)
                     /locus_tag="ACHALG_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFN2585866.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(70483..70767)
                     /locus_tag="ACHALG_000427"
     CDS             complement(70483..70767)
                     /locus_tag="ACHALG_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2585867.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
BASE COUNT        20501 a        15261 c        12638 g        23010 t
ORIGIN      
        1 acctccagac ttcttgatag ctcaagtata tcttgaggtg catttgttag taaggtactg
       61 ttctatgggg cgcttactta ataccgagaa aatggttttt gaacatcact tattcaaaat
      121 aaaaaaagat tgtgacacga attttgtcac aacctttaaa tgtattccta gagcaaagaa
      181 gctaagcgaa gaaatccaat tctgattatt tattgtattt ctttgttcag catccttgct
      241 aaaagcccat attggacaaa gtattcacta cacccatgat ctcatctttt cgagaacgtg
      301 ctttttctgt cggatcttct acctcttctg tgtcaattcg aacaataaat ttccgattca
      361 tcgtcacgta agcaccgtcg ttgatcaaaa taaacagttc gttctcatta tagtctgcac
      421 aggcatcttt ccatacgtca aagccgtttt tttcagaagg gatatcccgt ttaaccacag
      481 tttttccact taattcgtgg aacgttacgt cataatattt cattatctct acctctttcg
      541 tcattcaata ttttgtactt gttctctgat tttttctaag atcgtcttag actggacgac
      601 ttgattttta atttcgattg catttgattt ggaaccgatc gtgttgattt cccgattcat
      661 ttcttggatc aggaaatcta attcccgacc tacaggacat tgtgcttcga taagttcatc
      721 tagtttttgt acatggatcg ccagacgatc cagttcctcg tgaatatctc cgcgttccaa
      781 caagatggct agctccgtca acaaccgttg ctcatcgact ttcgctccaa gaaattcctc
      841 cagctttttt tgatacctta tttgaaattc cttttcatag atttctacaa aactatcgag
      901 tttttttagt acttctttga attcttcagt attctttttc aacactaaag ctaaagcttg
      961 accttctttt tctcgactcg cctcaataga atcaagtgcg cttttcaatg tatctagagc
     1021 taactgtgcc aattcttcca tgctctcttc cggtttttct tctaccgtta cgaagttttc
     1081 gtccagcgcg aagcgttccg ccatccgact aattgacact tgattttgac taccgtatcg
     1141 ttgttgaatt ccgtctgtca attgagtgat aagtgaatca atcaattccc agttgatgac
     1201 gatctgtttt tcgtttttgc caatataagc tagattcaca aatacttcga ttcgaccgcg
     1261 gcttaactgc tgtttaataa gctgacgaaa atcgttttca acaaagttca ggattttagg
     1321 acttcgtatt tgaacatcta gaaaacgctg gttgacactt tttatttcaa tatctaactg
     1381 atacgctttt gtttcgcgga tggctttgcc aaaaccagtc atacttttca ttcgacaggg
     1441 tccttcctca ttacatattt tctattgctt tttgcagttg ttcaaactct tcgttcgtca
     1501 atgtcaggcc tttccccatt ttttcatggt ctggcgccca atctcgaaga tcgaattttg
     1561 gcgggcgacc attccagctg actaggttca gttctttacg ccaccccttg ttgttttctg
     1621 acaatatagc gatttcttct acgatttcat atgaaaattc ttgtgccatg gttgttcctc
     1681 cttgagataa tataaatata ggatcagtaa tgtgtcaact ggttgcttaa gcaattcatg
     1741 ctgcaaacga tccttgcgtt ttccttgtag cagataatca aaataaacag ttttcgtgcg
     1801 ctcattttgt attttttgct ggtattttcg ataccttcgc cattcttcag tacttaattg
     1861 accaatttgt gccaataaat catccggcat actttttata tcttgttggc agatcatttg
     1921 aatcatcatg tctacttgat agatttcatt gggcagtaaa aaatgttttt ttgctaaaag
     1981 ttctagcatc gccaatccgc atgtctctgg tacgaccctg ttcttatata aattacgcag
     2041 tttttgccaa aattttatgc ttttgaaaaa agataattct aactgaattt cttttgttaa
     2101 gtaagacaaa aatcctcgcc agtatgttct tgcaggttta acagaatgaa atgtatacca
     2161 gttcccatag taatagccta atcgtttttt catttccaga tagcctaatt gtaaatttaa
     2221 ttggttcctt tggctatcaa aaagcagaaa gctgcctagt gtccacaatg tttgacattt
     2281 ccaatggata aaaatgtctg gcatacgaat ttttttcgct gggcctggcc cttgcacatc
     2341 tacgacaaat gcctctgttg ctcctttgtt aatcgcaata tcaataggta ttttgttgcg
     2401 atacccaccg tctgcatatt tattttttcc gatctttcga taagccatag cgggataaaa
     2461 tgaagcagag gcgagtatcc aatctggtat ttcttctgtt tttgtctgtt ggatatcagt
     2521 cactacttcc gctagttctg gcagtttagt agacaccgta tataaacgta ttttgggatt
     2581 atgtttgagt aaggacaaat ccaaattgtc tttgaccatc ttttccaatg gagcagaaga
     2641 aaccccgcct tcaattagtg ctgttttcgt catttggcgt ctttctcgat agaagcgttc
     2701 acgaaggtct tcaactgcag ccatttctgg taacgcaagc acttgtcttg tcgacagctt
     2761 tttccataaa ttcacagctt ttttcatatc tccttgtaaa atcaatgcgc cgttcaacgc
     2821 acctaccgag gtacctgtga taatctcaaa gttgatatca tgttccttca gcgcctgcca
     2881 cacaccaatc tgataagcac cgtgcgctcc tcctccacct aaaacaagtg ctgtatgata
     2941 gaccagcttt ttatttaatt gtgtggttcc ttgatagccg tttttatgaa aaatacgcaa
     3001 ccattcttct gatagaggag attgaaataa aaaacaacag ttttctttaa taaaaaaacg
     3061 agcgcaagct tccatatacc gaaaaaattt tttccattca aaagtttcac cttcaatcaa
     3121 cagattatta aaaataacat ctcctctact tttagcaata gtaaaataga cggtatattt
     3181 tttcgatact gctttaaaag cacattgctt ttttgcagaa cgccaaaaag cgtctctact
     3241 ttcttgatga ctttgaaaaa atggaagggg ttgttttgct tgctggatag gcgaatacag
     3301 cgacattttc tgtgaaaagt aaactttttt tatctccatc gcgctctcct ttttcttcaa
     3361 gtataccagt acgtcataaa aaggtcatga gtctcgtatt cgactttgta gattccataa
     3421 gaaaacaatt cataaaacct cctttaaata aaaaaataca aaaatcacaa aaattaattg
     3481 aaaatgagag tgttttcaat gaaaacgtga ttttgtaatg gaattttaaa ataaaagaaa
     3541 caagcttttt taatgcaaat gttgtaaaat aaaagagatt aagaggaaat aagaggagga
     3601 acatgatgac tgagtcagtt tatgtacata ttgataccac aagtaatgct gttttaacaa
     3661 aagggctgac tgctactgac tttgcaaaca gtatcgtgca ttttcctcaa aacttgctct
     3721 tgcttgatcc atctgcttct gctggagaat atgaatccca tacagggctg aaagtaatcc
     3781 gtggtacaga aaatattcag cgtttctttt cctcttctcg taacaggcgt ggattcgatg
     3841 aattgaagtg gatcgacttt actgatttaa cgatgctgaa agaattgaca cctttagaaa
     3901 tttcagaatt attatatttt ggtcatatga aaacacatct tcattcgccg tttttttata
     3961 aactacagaa taattttgta tacttcgatc ttaatgatca gctgaatcgg atttattatc
     4021 gctatttaga tgaattttat aggatttttg ctagtaaatt aagttcgatc ttgtcagata
     4081 agatcaatgt aaaaaaatct tttttcaaaa aagaaacacc tgttgaaaaa atcagtgtgg
     4141 aattattgaa agaaatgcgt aatttgatgc aggaagggat cattttttct tttgatcaag
     4201 ttggtttgat cgatggtaaa tatcgtatcc caatctatgt gattgaagat tatatttgga
     4261 aattaaaagg cacccggtat cgagaagagg aaatcatcgc tactttaatc tactctgtca
     4321 ccgatcgaca ctggcaattg gtagaagatg ccgatctttc ttatctgcca ttgaagtaga
     4381 tctatttctt atcaaaaaat aaaaaggaaa caagaaacta ggacatgatt ctgtctcaag
     4441 tttcttgctt cttttttatt tacagctcct gtcgtttatc tggcacaaaa atcgaaggag
     4501 tcgtttgttg gatccctaat aaaacgatga tcgttacaat agctgtactc aacccgctgg
     4561 caccattcat tacgaaagaa aagagccaag gattcatccc ccatagtgca tacgctcccc
     4621 agaaaaccac tcctgcaata aaatgccaaa aatatctgga aagtgcgcca atcaatgcac
     4681 caaacacgat gctttttcta atttgtttcg tattatcgtc tacatagcct ttttggacat
     4741 ttgaagccat gagccctgct gcgcctgcaa aagtataggc aatcgggtac tcaatcaaca
     4801 cttgaacgac acttaaataa taaacttgtc ccaacggaaa atgtaggatg ccccaaatga
     4861 aacctgccag catccctgcc tttgtccctc ttcttaacgc aaaaagagtc aatggaatct
     4921 gccctaatga aatagaaaaa ctgctgccga tctggatcgg aatcaacgac aacaccatcg
     4981 ctaacgctgc aacaatcgtt ccttctaccc aaactctatt ttttcccata aaaaaacttc
     5041 ctcctttgaa taaaaaagtc acaaaggagg aagcagcttt cgaaaatagt caaacagctg
     5101 tttattcaca attcctacgc tcgcataacc gatacaggta cgaagggttt agaaatagat
     5161 tcaatctcag cccaattgga ctcccctttg tgagtttatt tattatttat tttttgtttc
     5221 aaacaatgga ctaactggtt tgttttcatg gatacgtttg attgcatctc ccattagtcc
     5281 gccaacactt acttcatcga ttttgtcgat tttacgatct tctggaagat agatcgagtc
     5341 tgtaacaact agacgctcga tagctgaatc ttctattctt tgtaaagcag ggccggataa
     5401 tactggatgt gtacatgaag catacacact agttgcacca gcttctttca aagcattggc
     5461 agccaatgtg atcgtacctg ctgtatcgat catatcatcg atcagtacac aagttttgcc
     5521 tttgacttgt ccaatgatat tcattacttc tgcaacattt gcttttggac ggcgtttgtc
     5581 tatgatcgca atcggagatt tcaaaaattc cgctaattta cgtgcccgtg tgacaccacc
     5641 atggtctggt gaaacaacga caacatcatc gcctttgatt cctttttcta agaaataatt
     5701 ggcaataagt ggcgcaccca tcaaatgatc gactggaata tcaaagaaac cttggatttg
     5761 aactgcatga aggtccaaag tcagcattct tgttgcacca gctttttgaa tcatgtttgc
     5821 tactaatttc gctgtgattg gttcccgtgc acgtgcttta cggtcttgac gcgcatagcc
     5881 ataataaggc atgactacgt tgattgtttt tgcacttgct cgtttcagtg cgtcaatcat
     5941 gatcagcaac tccatcaagt tatcattgac tgggctgctc gttgattgaa taacatagac
     6001 atgcgatcca cgaatacttt cttcaatgtt tacttgaatt tctccatcac taaattgagt
     6061 aacagatgat tttcctaact cgacgccgac tgctgctgca attttctctg ctaatgggcg
     6121 attggaattt aacgcaaaaa tttttaatct tgggtcaaag taatgttttg acatggggac
     6181 ctccactttc tttttcacaa ctctagaatt ctacctaata aatagtagtc atatttttta
     6241 aataaatcaa gtgattttgc aaaagtctta gtttagataa gggagttttt ttgcatatcc
     6301 ttctttattg acttgtcttg ctctcgcaat tgccagcgca tattctggaa tattgtctgt
     6361 aattgttgac ccggcagcaa tcgaactgtt tttagctacc tcgacaggtc cgatgatatt
     6421 cgtgctagaa ccaataaagc tgtggtcgcc gacagtcgta tgatgtttgt tctttccatc
     6481 atagttgacg aagacaacac cacatccgac attgatttct tctcccagcg tagcatcacc
     6541 aacataagtc aagtgaccga ctttagttcc tttgccgatt tgagcatttt tcacttccac
     6601 aaagtttccg atatggacgt tttcgccaat ttcagctttt ggtcgtaaat gtgcataagg
     6661 tcccacatct gcatgtttct tgacatggct gctttcaatg actgagtttt caatgacgac
     6721 gtgatcctca atcctactgt ctacgatttt tgaatgtgac ccgatgacac aatcagaacc
     6781 aatgactgta tttccttgga tttgtacacc tgcttcgatc aatgtgtctg gaccgatttc
     6841 tacgcctgca tcgatgtatg tcgttgctga atcgacaaag cttacgccat tgaccatatg
     6901 catctggtta attcgctttc tcatgatctc atttgcttta gctaacgcga tccgatcatt
     6961 gactcccatt gattcttcaa aatcatccgt ttgataagca gcaactgtat ggccttcttc
     7021 ttttaagatt tcgatgatat ctgttagata gtattcacct tgagcattgt ttgttccaac
     7081 tttgcttaac gcatcgaaca aagcttcgtt gtcaaaacag taagttcctg tattgatttc
     7141 ttgtacaaga gcttcttctg gtgttgcatc tttttgttcc acgatttttt ctacgatccc
     7201 aatatggtca cggatgattc gtccatatcc tgttgggttt tctgcttgtg ccgtcaagat
     7261 cgttgcactt gcattttttc cttggtgata ttcaaaaaga ttgttcagcg tttcagtagt
     7321 caataatggt gtatcgccac tgataactaa cgttgttcct ttttttcctt ccaagaaaga
     7381 agcagcttgc aaaacagcat gtcctgttcc caattgttct gcttgtaacg catatttact
     7441 gcgttctcct agttgtgctt taacttgttc tgctccgtgt ccaacgatcg taatcacttc
     7501 atctggtttc gtttcactga cccgattaat gatatgttct accataggtt taccagaaac
     7561 gggatgtaaa actttatata attttgactt catgcgggta cctttccctg ccgccaaaat
     7621 aattgcataa cgtgcgtcca actgcgtcac tcctaaatat agtttgctga tttcagttta
     7681 tcccagtata cccatttttt caacaaaaca agtgattttc ttttatccca gcgttttctg
     7741 gataaaaaaa tagatagacc ataacgggcc tatctatttg gtaaccggtg ctctcctgcc
     7801 aactcctcgg tcacaagtca cactactcat aaaacatgaa aagctgtttt ttgaaatcgt
     7861 ttcttgtgac ttagagttaa acggtaggct tacaacctct tcattaaacg tttttcaatc
     7921 agctgacctt cggtattagc tcaataaacg gcaaaatatg aggagcaatt tttccgttta
     7981 ttgagctaat actcaggaca ggacgctgat ttcacaacct ctagataaac ggtgttcaaa
     8041 agccagtttc tcggtattaa gccaaattta tgaaaaaatg tggaaagcca ttttcacaaa
     8101 atttttctta ctactcgaaa ctaagcagct ttttcacaac ctcttattct gaaaaataat
     8161 ttcctggcac aaccgtaata tttttcgttt gagtatctac atctttgaca taaagcagtg
     8221 acgtataatc atcgatggcg cgatgaccgt tgaattttga ttcggcaaat acagtgatgc
     8281 caacaagttc tgattcgaat tcttcaatca agctcttcat tccgttgact gttccgccgc
     8341 ctttcatgaa atcatccact acaaggacac gtgaaccacg tttcaaactt ctcttagaca
     8401 gttccatctt ttcaattctt tcagaagatc cagacacata gttgacactt actgttgatc
     8461 cttctgttat ttttgagtcc cggcgtacaa taacgaatgg cgcattcaga taataggaca
     8521 cagcttgggc aataggtacg ccttttgtgg ctacagtcat tacagcatcg attttttcac
     8581 caaggtattt agaagcaatg attcttccaa cttgacgcag cagttcaggt tctcctaaca
     8641 aatcagagag ataaacatat cctcctggaa gcagacggtc ttgttctgat agacgttctg
     8701 ctagagattc aatatatttt ttcgcctctt catatgaaat ttcagggatg aacaatacac
     8761 cacctgcagc acccgggatt gtttccaagg ttccataccc tcgttcttta aatgttttct
     8821 tgatgattcc caaatcttca ctgatagaag atttagcaga ttcataacgt tcggcaaagc
     8881 ttgttaagga aatcaattca tgcgggtgat tcaataaata ctgtgtcatg tcaactagac
     8941 gttcactgcg acgtactttc acctattcca actcctttta agaaatttta tcgttttaca
     9001 ttatataagt ttttcttcaa aaattcgagc attttatgaa aaaataagaa aatagttcgg
     9061 tatttattaa aataaaaaga agcctataaa aaatttttcg tttttataag gcttcttttc
     9121 tttataattt agttattttt tcttctgaag gcaaccaata ttcgtttgat gatatttaca
     9181 atcaggaaca aagcaataaa gactaatgtg attgttgctc ctggaggtgt atctagataa
     9241 aacgagctag ttagtcctga cagcatccct agaaatccta cacaaacact gatagcaatc
     9301 acgacattga agcttttgcc taatcgcata ccgattgctg cgggaagaac catgattgcg
     9361 gatatcaaaa gtgcaccggc aatcgggatc atcacagcaa tagccacacc agtaatcaca
     9421 ttgaacatca tcgacatcaa atgaacgggt agcccatcca cgtgggcggt atcttcatca
     9481 aaagtcagaa catacatcgg ccgtttgaat aaaaagaaca atataccgat ggcaacgaat
     9541 aagattccta aaaagatgac ttgctcccat gtaatggtca cgatcgaacc aaacagatag
     9601 gattggatac ttgttgctga attccctccg ctcaaattca tcaatactaa cgctagcgcc
     9661 aaaccaccag ccattaaaat ggcaatggag atttctgaat aggttcggta gatcgttcgc
     9721 agatactcca atatcacagc tgctaataca acgactagca tggtcgtaag tgtaggatta
     9781 aggttcaaaa agaatcctaa cgctacccct gctaaagaga catgagaaag cgtatcagcc
     9841 atcagcgatt gtcttctgat cactaaaaac acacctagca tcggggcgat tccggcaatg
     9901 aagatagcgg caagaaacgc tcttctcatg aattcatatg aaagcaacgc cattgagaat
     9961 cctcctttct caccagtcgg atctgtctgt ccgcatactg tttgatatct tcatgatcat
    10021 gggtgatcat tagaactgat ttcccatgga catgcgcact atgtcggaga agacgataaa
    10081 attcatttct ggattcttca tccatacctg ttgttggttc atcaagaaca aataagtcag
    10141 ggtcagtagc aaaaatacgc gctaaactga ttcgctgctt ttgtccgcca gacaattcac
    10201 ctattcgccg atttcgcata tcccacatcc ctacagcttc taaagcacgc tgtacatgtt
    10261 gatgatcttt ttcagagaga cgcttgaacc agcggcctct tggatatctt cctgaacgga
    10321 ctaattcgat caccgtacta ggaaaacctg cattgaaaga tgcaacttgt tgaggaatat
    10381 aaccgatact taattttttt ccttcttgat ttttctcagc aatctttatc attccttttg
    10441 aaggtttcag taatcctaac gatgctttca ccaaggttga cttggctgct ccattttctc
    10501 cagttaaaat gacaaattca cccgcatcta catgataaga gacatcttct agtacaggtt
    10561 catcatcata gtaaaaggtc aaatcagata tttcgatata acgcacctta caacctccat
    10621 tctaaaactt aattgatact ttcttttaaa gctttcagat ttttttccat gacagaaata
    10681 taattttcgc ctgctttgat ttgttcatta gtcaagcttt ctaaaggatt caacacttct
    10741 agcttcactc ctgtttcatt cgacaatgtc tcagctacct tcgaagaggc attttcttca
    10801 aaataaatca ctttgatgtt gtttttcttc acaaattctt ttagctcagc taagcgactt
    10861 ggtgtgggtt cctgatctgg tgaaacgccc gaaatcgcta cttgttttaa gtcgtattga
    10921 ttcgctaaat aagcaaaagc agcatgctga gtgacaaatg tgcggtttgt tgcatctttt
    10981 agctcatcgg agtatttttg atccagtgct tcaagctttt tgatataagc atcggcattc
    11041 ttcgtaaaag tttccttttt atctggatat ttttcgccta attcttcagc aatcgttctg
    11101 acttctttta tagctaatac cggatcgagc caaacatgtg gatcgtattc atgtgaatga
    11161 ccatgccctt cttctgattc ttcatgttcg tctgcttctt gcgattctaa acggtcgatt
    11221 cctttagctg cttcgatcat caaggtctgt ttgctatcca ctgaatcctt taacgaatcc
    11281 acaaaaaatt ccatgtctga actattatac acgaaaacat ctgcatcgga aatttctgct
    11341 ctttcttttg cacttggttc gaaatcatgc ggctccgttc cagcagggat caaaagtttc
    11401 acgtttcctt catcccctac gatctctttt gtaaaatcat acatcggata aaaagtcgta
    11461 acgatcgtca aatcctcttt tttgtctgct tccttgttcg tatttccaca tgctgcaaac
    11521 agtaaagcac ctatcattac cgtagctgtc aaaagatatt ttttcattta tatgctcctt
    11581 tcaatgaaca aatcggaatt ttacgattta ttaacgtgaa ctaatgtatc aaaataaaaa
    11641 aacacagtca atagaaatcc atcaatttct gatttaagca tgtatattct tgattaaatc
    11701 gtaaaatgga tgagttgctg ctcatatcta gccatttcac tacaataaaa aactgaagaa
    11761 taccatttta ggattcctca gtttttcatc acctatttta cttcttatat tttattgcag
    11821 ggttcgtact aaataaactt cctcacaaaa accttttaac ccattatata ctctctgcgc
    11881 tcttgaataa tgttgacaca aggcaaaaac ggttggtccg cttccactca tcaatgcagc
    11941 atctgctccg tacttgatca tacgctcttt gatttgatgg atgactggat gccgctttcc
    12001 cgttatatct tctaaactgt ttcccatttt tttgatcatc tgctgataat cttgctctcg
    12061 gatggcttca gcaagtccgt ggatgtcttg atgttggatg gcttgcgtat ctacttctct
    12121 aaacactgta cgtgtagaga cactcattct tggtttgacc aacacaaccc agcactgtgg
    12181 catagaaggt aatacttgga tttcttcccc tttgccaccg acaaatgccg tacttccgta
    12241 gacacagtaa ggaacatctg taccgacttc caaaccgatt tctgccagtt cttctaacga
    12301 acagcccaga ttccataatt ggttgatccc tcttagagca gctgcacagt ctgtacttcc
    12361 tccgcctaat ccagctgcta ctggaatatt cttcttaaca aagatcttga caccggtttt
    12421 gatattgtat ttatttttta gcaataaagc agcttgataa acattgttcc gttcatctac
    12481 cggtaaaaat gctttatttg tttctacaat gattttgtct tctgttattt cttcaaaaaa
    12541 tagatgatct gccaagtcta cgctcgacat caccatttcc aactcatgat acccatcttg
    12601 ccgtctatat aatacatcca gtcctaaatt tatcttggct ggcgcttttt caatcaattc
    12661 catcctctca cccattcgaa aagtctaagt cgcaagtatt ctatcatagg gaaaaataaa
    12721 agtatagctt atgtctgcct agtggttgat tttgtcatcg tttcaacagc tttcacagaa
    12781 ttttctcttc ataatccaaa caagttctag tgattcttat aattctgtga gcacttcttc
    12841 tgccaaactc ggaacatttc ctttttcttt tcctatcaga ctgagtagct gcatggtagc
    12901 aaatccgaaa ttacttcctt tcctataagc aaaataacgt ccttcccaat gatcgacata
    12961 ttttaatttc ttttcataag cagactggaa ggaatagaaa aaattaccat aattatatac
    13021 gatatgcatg actttttctt gaaagaaaga aaaatctgtt tctccaacat tagctaatgg
    13081 agctaagcct agattggctt ccgagtagcc cattttctcg tatacaataa acagattggc
    13141 taacagatag tcagctaatt cctcagattc tttaggtaag acccgcaaca aatcatatcc
    13201 cgcctgcttc ttcgtcatcg gctgagcggt gatgaagcca acgacttttt gatctttacg
    13261 gacaataccg acaccgctag ataatacata tgatttatca aatcgtccca cagagaagta
    13321 tttttctcgt gcaccttgaa gccatttttg tgaaacttca tttacagctt gatgtaattc
    13381 ttccggcagt tgatcgtacc aagtaaaatg gtatccttct ttcatcaaac gttgatattc
    13441 aagttgctga ctcagccgag acaagtcttc tgttgctaga tcaacaatcc ctgcttcacc
    13501 tactttcgtg aagtgaaaac ccaattcatg aagcatcact acgtagcttt ctgatatctt
    13561 ataaaacgca agctgatagc ctaaacaatc tgcttgtttc ataaactgat tcgttgctgc
    13621 ccgcatcatt ttaggattgc caactggatc tcctaaaaca aagcatttgt ttcctttgat
    13681 ctgataacca aaaacgactt gatcttggtc gtcttcttgg taataaaaat aactgtaacc
    13741 tgacaagcga agctgatggc ttgtagctgt gcctccgtat gtttccaata aacggtcaaa
    13801 acggtctttt tcgtactcac tgccaagctt cgtagtatct tcagctaaat attgataaag
    13861 cgccactaaa gctagcaatg agatgcccaa accaataagc cctgagaacc acacatcatc
    13921 tgaaggaaac aaaataaaat gatttcctgt tattcgaccg actaaaaagc gtccgctgtg
    13981 atagcctgca attgcgtaaa cgataaacaa aaagccgaac aagctagcat caaagattat
    14041 agctccccaa gaataaacga attttgttcg ataaaattct tttctggata accacacagc
    14101 taacagaatc aagacatata ccaatatcaa ctgccaagat aacgtacgtg tgattgtatt
    14161 taaaatacca aaagatagta aagcaatcgt aggcagaaat gcctttttga cttttactga
    14221 aagtgctcta gcaaggccga gtaataaaaa gccaaccaat agattcactg tctgatctaa
    14281 aaaattgaat gagaacggca ataatacttg aaatagccga ctgattgcag acagattagt
    14341 gattgttgat agaagtacca tcatgatccc cgcaaaatag acagcactta cgagcacgat
    14401 atgcgcaacc ttttggaaaa acagtcgcgg aatgttatct aaaaatcgat tgattttgac
    14461 tcctgtatgt gtgatgaaca atagaagtcc cgtcataaaa ggtagaacat aataaaacaa
    14521 ccgataatat aatagccaaa ctactgctac atcttgccga acaggcaatt gactcattcc
    14581 taagatcatc aaaacgtcaa aagtccccat tccacctggg accatcgtca gcattcctat
    14641 caatgtagca atcatgaaca taggatagat cgaaagcatc gaaatatcta ttctcatcaa
    14701 cttgccgatg atcaaaaata ccgcaagggc tcccgtccat tgtccaaagg atgccagtag
    14761 cagaccaagc aagccttttg gataaaactc gaagaacaat gtctttctct ttagataagc
    14821 aaaaaagaaa atgactggtg ctaaaaaact accgccgaaa agccagatcc aatagcttcg
    14881 aaaaatactt tctgactgaa taaaaaagac atcaataaat gtaagaaacg accatacaga
    14941 caaaccagtc agcataaata gtgcgacttt tgatacagtg gctaaaacca tttttctatt
    15001 tgtctgcttt ccataaaaac tagcccgcag acttgctcct accacaccac caaaaccggc
    15061 gagattgttg atcgtgtttg ttacccacgc agcaagaaga aactcttttc tttccatttt
    15121 tggttttcct tgtttttcta agactctgat cgtcacccaa tcatacaaca acatcggagt
    15181 gacacccagc agtccagcca gaatcatgag caggatcgtc atgcggcttt gttgtcccat
    15241 cctttgccaa atatcttgcc aactcatgcc attagcaata tttgcgactt gatttgccac
    15301 aaagactaac acggagccga aaaaaatcag ttttaaaatc agactatgtt cctttatcca
    15361 ttgcagcatc aagtatcgtt tatttttcac attttcccat tcctaacttg tatacttttg
    15421 tgtcataccc caatgttatc ttatttattt taatttttcc actttagacc tgttctttgc
    15481 ttgcgatgat agaaaaagaa agatatccta atgctaaagg agaggataac atgaaagtag
    15541 gatttatcgg cacagggaat atggcacaag ctattattga tggtttatta aaaaaaggaa
    15601 cagtctcgaa agaagaggtg ttcgtttcca gtggacattt tgaaaatgca caaaaatttg
    15661 ctgctgaaac tggcgtccat gcatgcaaaa gtaatcagga agttgcatca caatcagata
    15721 tcgttctttt agccgtgaaa ccaaaaatca ttgaaaaagt attggaagaa ctaagagaat
    15781 atagccagaa agtcttattc atttctattg cttcaggaaa atcattaaca gatttagaaa
    15841 cagcgcttga agatcctcag gcctccatcg tacgtgtgat gccgaatgtc aatgtcagcg
    15901 taggagcagg cgtttctgcg atttgcaaaa atgaagctgc cacggaggaa cagcttcagc
    15961 aagctacgat gatttttgaa gcaatcggca gcgtctatcc tttagcagaa tcagatttca
    16021 gtacattcat tggccttgct ggcagctcac ccgcattcgt ttacatgctg atcgatgcga
    16081 tgggacggac tggtgtactg aatggtttgt ccaaacagac agccactgag attgctgcaa
    16141 aagcaattat gggttcttgt caaaaattcc tttcaagtga taaaaatcca tgggaacttg
    16201 tagaccaagt ttcttctcct ggcggtacaa ctgttgcagg tgtagttgcc ttagaagaag
    16261 caggattcat cccagcaatc atcaaaggaa tcaatacgac gatcgagaaa gatcttgaga
    16321 tgttgaataa aaaataatga tagcaatgtt ttttcgtgcc ttacaaaaaa agaagagctg
    16381 tggcttttgc cgcggctctt cttttgtatt ttttattatt tagcataatc agttgcacga
    16441 gtttcccgaa taacgattac tttgatgtgt ccaggataat ccagttcttc ttcgattcgt
    16501 ttacgaatat cacgtactaa acgaacagcg tctaaatccg agatttcttc tggcttgacc
    16561 attacccgaa cttcgcgtcc agcttgaact gcgtaacttg attctacacc ggcgaagctg
    16621 ttcgaaatat tttccagact tcttaaacga cggatgtagt tttctaatga ttcacttcgc
    16681 gcaccaggtc ttgctgctga gagcgcatct gctgcagcta caaggacaga gataacagaa
    16741 gtagcttcca catctccatg atgagaagcg atcgcgttga ttactactgg gttttctttg
    16801 tacttggcag ctaattccgc accgatctca acgtgagatc cttcaatctc atggtcaagt
    16861 gctttcccaa tatcatggag caacccggca cgttttgcta attggatatc ttcgccaagc
    16921 tctgctgcta agataccagc taattttgca acctcgaccg aatgcttcaa gacgttttgt
    16981 ccataactag ttctgaatcg cagacgtccg atgatcttga ttagatctgg atgaagtgta
    17041 tgagcaccga cttcaaacgc tgcctcttcc ccgtattcgc gaatacgttc atccatttct
    17101 ttacgagatt tctcgaccat ttcttcgata cgcgctggat ggattcgtcc atcttgtatc
    17161 agtttttcca aggtcatccg tgcgatttct cttcggattg gatcgaatcc ggaaagaaca
    17221 actgcttccg gtgtatcatc gatgattaaa tcgataccag tcagcgtctc aagcgttcga
    17281 atattacgac cttctcgtcc aatgatacga cctttcattt catcattcgg aagggtaacg
    17341 actgaaacag tcgtttctga aacttgatct gctgcgcagc gttgaatcgc taatgataat
    17401 aagtttttcg ctttgcgatc tgcttcttct ttggcacgct gttcagattc ttttaccatt
    17461 agagttaact catgattcag ctcttcttca gtcgatttca tgatgacatc tcgtgcgtcc
    17521 tcttttgata aaccagcaat tttttctagt tctttttgtt gatcttcaat cattttttca
    17581 acgtcttttt ctcgctcttc aattaattgc tgttttgaac taagtgcttc ttctttactt
    17641 tcaagtgagt tctcgcgttt ttctaacgag tcatctttac gattcagcgt ttgttcgcgt
    17701 tgaatcaaac gattctcttg agattttaat tctaacttac tttctctcag ctcactttca
    17761 acttcagaac ggtatttctg attttcttcc ttagcttcta acaatgcttc tttttttaga
    17821 gtttctgctt ctttttttgc actatcgatg attccagcag ctgatgattg tgcaccagct
    17881 atttctttct cgtgacgtga ctttgcgacc ataaacccta aaccaagacc gacaattaaa
    17941 ccgatgatag cgaggagaat gttgaatacc ataaaatcca cctccatact atctttttca
    18001 tttttatcat ctatacagct tctatgataa attattctaa actcgtcaat atgcatcttc
    18061 gcatatatgt gtacaaaaaa catgcacaac tttattctaa tgtttgtacc ccgtagtgtc
    18121 aactttaaat aaacaaagaa acggctttct acttattcat taaacgatgg tttttaaaat
    18181 aaatgacact agtttcatat aggtaaacct ttaaattgag aaacttttct attaaattat
    18241 tctttgtaga tgaacagata tttttatttt atgtacccac tcgtataatt aaataactca
    18301 cgatttcttt attcaataaa aaaacggcag gtcaaggaga gtacctactc ttcagaccag
    18361 ccgtttttgt ttatcataat ttttattctt ctactaaagg caattcttct tgtccttttt
    18421 catcttctgg gacagcgact tcttcaccaa taccataagc tgctctaact agagcagaaa
    18481 cttctgccat catttcagga tgattagcca tatagatttt agcgttttcc cgtccttgac
    18541 caatccgatc ttctttataa gaataccaag caccgctttt atcaacaatg tctttttcta
    18601 ctgccatgtc tagaagttct ccttcttgtg agatccctaa accatacatg acatctactt
    18661 cagcgatttt gaaaggaggt gctactttgt tttttacgac tttgatcttt gtacggttac
    18721 caacaatatc tgtaccttgt ttcaattgtt ctgcacgacg tacttccaaa cggatcgttg
    18781 cgtagaattt caatgcgcgt cctccaggag tgatttctgg attcccaaac atcacgccta
    18841 ctttttcacg gatttggttg atgaaaatag cgattgtttt tgttttgttg atcgaaccag
    18901 agagtttacg caatgcttga gacatcaaac gtgcttgtaa cccgacatgt gagtcaccca
    18961 tttcgccgtc gatttctgct cgtggaacta aagcagcaac tgaatcgact actacgatat
    19021 ctacagcccc acttgatact aaagcatcag cgatctctag accttgttct cctgtgtcag
    19081 gctgtgaaag aagtagttca tcgatattca cacctaattt ttgtgcatat tgcggatcta
    19141 acgcatgctc agcatcaatg aaagcggccg ttccgccatt tttttgtact tctgcaatag
    19201 cgtgtagtgc aactgttgtt ttacctgaac tttcaggtcc atatacttcg atgatacgtc
    19261 cacgggggta accgccgaca cctaaagcga catctaacgc aagagagcca cttggaattg
    19321 tagagatttg ttggtcgatt ttttcgccca atttcataat agagccttta ccatagctct
    19381 tttcaatttt tttcagtgca gcatctaagg ctgctttacg atcatctgcc aatacgataa
    19441 tcctccttat atttacaaat cagagcaaca tttcattatc cttatcatat cttttttagt
    19501 gaaaaaaagc aagaaaaaac cgaacaaata ttcgcattta tcattttctt aaaagctgtt
    19561 ttctcaaaag atcaaatcct tgcataaccg cattatatct gatagcagca cgatctctgg
    19621 caaagtgata tttttccgaa accacgccgt ctttagatga aatagcgatc catactgttc
    19681 ctgcagattg accttctaac gtgtccggtc cagccactcc ggtgaaagct acactgtaat
    19741 ctgttcttgc tatctcacgt gcttgaatgg ccatctgctc cacacattct ttgctgactg
    19801 ttccatattc tgctaatagc tccggatcaa tctccagaaa accagctttt gtttggagcg
    19861 agtaggtaac gaatccgcct ggaaatactt cagatacacc agcaatgtcg cctaatgcgg
    19921 cttggaaggc gccggctgtc aaactttccg ctgctgttac agtctgcttg ttttctttca
    19981 gtagttccac tacgactttt gctagcgaat tatcatcgcc gtatccataa aaatattccc
    20041 ctactcgttc ttggattttt tcttctaatg ctaaaagagc ttgattccct gcctgaacat
    20101 catttgtttt cactgtcagt cgtaatgtca cttcatttgg tttggcatat ggtgcgatcg
    20161 ttggattgat ctgtgtctca atcagatctt ttagctcagt gaccagtcgc gattcaccaa
    20221 tcccataaaa gcgcaaaact ttagaaatta gtttttcttc agaaggaaaa cgttcctcta
    20281 gtaaaggacg tacttgttcc acaaacattg gtttcaactc attaggcgga ccgggaagaa
    20341 ggatatacgc atgtgtatct gtttgataaa aagtccctaa agcaagccct gtacgatttg
    20401 gtagaggaac cccgccttcg ataatctggg actgttgcag attgtttttg gtcatttttc
    20461 gatgggttgt ttcaaaatat gccaataatt ttttgtatcc ttccgtattt tgtatgagag
    20521 acttaccgag gtgagccgca gttgcttctt ttgtcaggtc atcttctgtc ggtcctaatc
    20581 cgccacataa gagtatgagt tcacttcttg tttcagctag agcaatcaac tcttctaaac
    20641 gctgctgatt atctcctaca actgtttgat aataaacttc gatacctaga tcagctagct
    20701 gttctgacaa gaaagtggca ttcgtattca cgacttgtcc tagtaaaagt tctgttccga
    20761 cagctattat ttctgctttc attacttccc gctcccttat atagtatacg ctgtattttt
    20821 atattttttt atttaaaaag agggcaagga gctgtgacaa aaatcttgtc acagctcctt
    20881 gttccaaata aacagtgttc tcctgccaaa acctcggtca taagtcacaa tatacataaa
    20941 acatgaaaag ctgttttttg aaattatccc ttgtgactca gagttagacg gcaggctcac
    21001 aacctctcca ctaaacgata cttacattga tcctttgaac acaccgctgt ttttgatgaa
    21061 gtaatccaca cctgaataga tagtaaagat cagacaagca taaagcatga tttgatctac
    21121 tggaataccg atcaggctga atgggatatt attgataaac agcaagataa tagcaaccat
    21181 ttgtgtagcc gtcttgactt ttcccggcca agctgcagcc atgacttctc catgttcgac
    21241 aagtagtaaa cgcaagcctg tgacggctaa ttcacgacag acaatgatcg ctactaccca
    21301 accaggggcc tttccttggt caaccaacat gataaaagca gtcataacta gcattttatc
    21361 tgccaacggg tccgcaaact tgccaaaatt agtgacaagg cctcgcgcac gggcaatttt
    21421 tccatctaac cagtctgtga tactagctaa agcaaaaata actgcaccta ctagctgtgt
    21481 gacagccagt gtggtgtctc ctacgttaaa ttgtccccaa tctaaaggta aacctacaac
    21541 caaaatgaaa atcgggatca tacaaattct aagtacagtt aatttatttg gtaaattcac
    21601 actatcgact cctttcttcc ctattctacg tgactatcag cgaagtttcc accgatatta
    21661 tttacttttc caaatgaatt tattcaaagg agtattttta ttgaaattga tctttttgaa
    21721 ttaacgaaga aggaccgcga cacagatttg tcacgatcct tcttttttat acctacaagt
    21781 aaacagatgc gtaaactaat ttatacgatc attctgcgta ttgaagagta aggttcacat
    21841 tttttggact ctgattgttt tcccctggat taactggtac ggattctcca ttcgctttga
    21901 tcgttgcatt gcttgctgca ccaatcgtga tggtcgcatt tgttgctgtt tccggtaaca
    21961 cggtactctg tgtttcattt gctgccagtg tcccttgata aacataggca ttgtctacta
    22021 gcacacctaa ccatacacga ttgacagcag taaattcaag ggttacaggc ttggtagcgt
    22081 ctgtgatatt tactgcgatg gcactgccag aatcttctcc tgtagctatt gccattttct
    22141 tttcttcact tgattttgtt gttgattcta ctgtagaaga ctctttggtc tgcgtgcttt
    22201 gctctgagga agaggactcc tcgactgatc cttctacttt aagcgaagaa gctgtgtctc
    22261 cgattattgg atcagcttga cgatcttgcc aagtcatata gcccacgaca acgacaatcg
    22321 ccaaagcaac caatcctaat aaaatgacag gaagacttgt ccaaaagcga cttggatgcg
    22381 attcttcctg atgcattgct tttctagaag tacttactgt ttcaggctgc ggacgttcag
    22441 ggaccgatcc tgtcaatttg cttttcccat caaaaacatc gactaaatgg tctccatctt
    22501 ctcctacagc ttcagcgtat tgccgaataa atgcccgcac gtaaaattta ccaggcaatt
    22561 ggtcaaaatc atcattgtcg attgcttcca aataacgttt ttgaattttt gtaatctgct
    22621 gtaattcatc taaagatatt tttttattca ggcgtgcttg ccgtaatttt tctccgatgg
    22681 tttcaaaagc cactcgttca tcttctccag tctgtttgat tctccgaagc atctgctgtt
    22741 gcaacgaaag acgttatgtt tgggtgctgt gacaaaagtc atgtcacagc tccttttttc
    22801 cgaataaaca ttgttctcct gccaactcct cggtcacaag tcacaatatc caataaatat
    22861 aagaagctgt tttttgaact tgttccttgt gactcggagt taaacggcag gctcacgacc
    22921 tctttcttaa acggtgttca atcagctgtt cctcggtatt agctcaaaaa ccggcaaaat
    22981 ataaggagat atttttccgt atgtttctct aatactcagg tcaaaacgct gatttcacaa
    23041 cctcttcctt aaacggtgtt caaaagcttg catctgcggt aataagtcca accaagttaa
    23101 aaacattaag aagctgtttt tacttggctg ttcttattac ttgatgcagg gcagcttttt
    23161 cacaacctct tggtacacgg tgttccagaa gtagcttctt cggcaataag ccgaaatatc
    23221 acaaaaattt gagaagcaat tttcgtaaat tccttcttat ttcttgaagc tgaccacttc
    23281 tgtcacaacc tctttgatta cttcgtttga ttttatttta atataagttg attaaaagag
    23341 agcatgattt gttttatttt ctcactaatt tttccaacac aactaagaat accataaaag
    23401 ctgattttgg aggaggttcc tactacaagt ctaaaacgaa atttaagact tttcctcata
    23461 aaatactggt ttattttggt ctcatgtaaa actcgctgaa ggcctcttca cttataaatg
    23521 cttccccagc tgccagtacg tctttcattt ggatcgaatc gatgatttca ggaagatcaa
    23581 ataaagttcg atcgccaaat aaactttgcg taaattgatt ggcaatatat tcgatcgaat
    23641 ttaatgattg gaaatattgt ccaagcattt tcttcttcag caaatcgaga tttgtttctg
    23701 acacttccgg atcatcagcg aatccaagaa tgatttcttt gactttttct gctgctttct
    23761 ctggttcgtc cgtatctccg ctgaagtccg caaagtgaaa ttcacgatcc aagctgaatt
    23821 caaaaccaaa gctgtcatcg ataattcctt gattgtacat cgctagatag tttcgagaag
    23881 tattgcctaa aatcatttgg aacaacagat tgattgctgt tttatagcgt aacagttctg
    23941 ttccttcttg cggcaatgtg tctaatcctt taataccaag tacaaattta tctcgtgtaa
    24001 tggctgcttc caatgcactt tgtttgatga tttcttttgt gttttcaggg aaataacgta
    24061 caatttcttg ctttggaggg aaattttttg cttcttggtt ttcccgaata agtttcatca
    24121 atttctcagg ctccatctta ccaactacaa ataaaaccat attgcttggc tgataaaacg
    24181 tccgatagca cgtatacaag tcttgagctg tgattttgtc gatactttca accgtgcccg
    24241 caatatcgat atgcaatggg tgtgtaggat acaagttatt tagtatgcca aaaaacatcc
    24301 gccagttagg gtcgtcttca tacatttgga tttcctgacc gatgattcct ttttccttgt
    24361 ttacagtttc ttctgtgaag taaggtgctt gtacaaaatc gatcagagtc gtcagattct
    24421 tttctacctg atccgtcgta gaaaacagat agctggtttt cgtaaaactt gtgaaagcat
    24481 tggcggaagc tccttgtttc ccaaataatt gaaagacatc accatcttct ttttcaaaca
    24541 acttatgttc taaaaaatgg gcaattccat ctggaacttt caccttttct ttttcaccat
    24601 agggaataaa ttcgttatcg atcgaaccat aattcgtact gaacaaacca tatgtcttgt
    24661 gatagtcatt tttaggcaac aaatacacgg tcaaaccgtt tggcagtact tcatgataaa
    24721 gtgtttcatt gatttgctca tattctgttt tattcatttg catttcctcc tgctaaaaag
    24781 aagattgcct gcaattcgat ttgctcagct acttgttgta tctctttgat cgttacgctc
    24841 tccatacgtt tcaaccattc ttcatcagat aattttgttt ccggcagcca gctgtccaaa
    24901 aaagaagctt cgattgccgc ttgcggagag tctagagaaa gcagatattg gtttctcagc
    24961 attgcttttg tctgagacag ttccaactct gttatttcac catttcgcaa actttctaat
    25021 tgctcgtgaa tcagatgaag tacttgattt cggttctttt catcgatacc ggtttggaca
    25081 ctcatgaatc ctcggaacgt atccacgcta ctagaggcat aataagctag actttctttc
    25141 tctctgacat tcatgaaaag tttagagtga ggaaaaccgc caaataaacc gttaaagacc
    25201 attagcgcaa aacgttctgg ttcatcgtaa tacacatttg tctgataagc taggttcagc
    25261 tttgcttgaa cgattggctc acgtacttgt cgttccttca cgatattcac ttgaggttgg
    25321 gtataaaaca gatctggata aactgtttcc cgcgactcaa atggtaattg cccaactgct
    25381 tcagctgctt tgtccggatc aacatcgccg acaacaaaaa tgtcgatttg atcatttgcc
    25441 atcatttctt gatagtagga cgctagcgaa ctagcagtga tcttgtccaa atcttcgact
    25501 acgcccaacc ctgggatctt ttgttccggg ctgttttcaa aataaagttc ttgcaaggca
    25561 taggaagcaa aagtttgttt atcttccttc aaactttcca aatatagacg cagattgttc
    25621 ttttccaagt caaacgtcaa ctgatcaaac tgatgatttt taatatttgg ataaaataaa
    25681 acctctttta aaaaatctac cgcttgagaa aaaaggtttg gatcattgac atattcacca
    25741 ttcacaaaac tgatccctgc gtttagccaa tgtaaatttc ctttttttct aactgataat
    25801 ccaaagcttg cgccataaag ctctgcaagt tgcgcactta actttgtctg atccggataa
    25861 ttcaaactat ttgtttccaa catgctgctg agaagtgaac gtttcgtgat gacttcttgt
    25921 tgcagacgcg cagtaaaacg gataaaaatc cggatcgttt tgtattgttt tgttggtaag
    25981 acatgaagat tcacgccttc tgccaattga tacaccatat ttatatgctc cttttcacag
    26041 ttgattgccc gcttactctt ttaatgggtc aacctgcctt ttttacttat tcctctgctt
    26101 gaaatttata agttcaggca attcgccgtt ttttctaaat gatgttacac tttatagtat
    26161 agcatagatg ttttttatca aaagaaacag acggctaaaa gcgtatattg tttttttatt
    26221 ttttattttt cgatatacta aagacaaata aaagaaggga gggtcattca tgatcaaaga
    26281 attcaaagag ttcatcatgc gtggagatgt cttggatctt gctgtcggcg ttgttattgg
    26341 tagtgcgttt acaagcatcg taaaccaagt ggtgcaaggc ttgatcacac cactaatagg
    26401 ttggtttgtc gcaatgatca caggtacttc tgatttagaa ggagcacttt caattcttga
    26461 ctggtcgccg acaaaaggcg tcacatttgc gtttggtaac gttattagtg ctatcattac
    26521 atttctgatc actggttttg tcttattctt agttgtcaaa gcggcaaacc gtgcgaagaa
    26581 tttacgctcc aaagaagagc aagtggaagt ggaagaaact cctactgcag aagattatct
    26641 tagtgacata cgcgatcttc ttgccaaaca agtggcagaa gacaatacac atccagtaga
    26701 gcaaacaaat ctttccgata cttctaaata ataaagaatc taaaaaaaag aaagccgatt
    26761 ttcagtgtcg gctttctttt tttagattct taacttgcta cttgttcctt tttcatgtcg
    26821 atatgtggaa tatcatcttc caaatacaca tctgagattg ccttgaatcc aaacgactca
    26881 taaaaagctt gtaggtattc ttgtgcgccg atcacgattg gtttttctgg aaaatttttc
    26941 tggatcatgt taatcgtttc ttgaacaatc tttcttccta aaccatcttt acggttgtcc
    27001 tgatgaacaa ggacacgtcc aaaatggata ctctccgctt cttcatatac tcgcgtgtag
    27061 gcaaggatct tcccatcagt atcttgaaaa aacgtatgcc aagcttgtcc atcaatgtca
    27121 tcaacttctt gataaggaca ctgttgttcc acaacaaaaa ctgcgattcg ttctttgacg
    27181 atcgcgtaaa actctgctgt tgacaattct tcaaatttct ttacctgata ttccactaag
    27241 caaacgcccc ctgctttttt attcttagca taccgattcg cacacaaaaa agcaaacgaa
    27301 ccttacgaat ctcaggctcg tttgcttttt aaactgcttc agttaaaaat tcgatctcaa
    27361 ctgtccgagt aagtacatct gaatagctgt aagatactcg ttcaaaagaa ttttcttcag
    27421 gatccaaatc aacaacgaat actgaaggat aggtttctgt taaaacgccg cggcgttctg
    27481 tttgacgctt tcttcctgtc tgagcaacca acatgattgg gctgccgata cgaccttcta
    27541 aatctttctt gattgaagct aaagttgttg gcatacagtt cacctctact atcaactata
    27601 ccacatattt ttaaaatttt caagtttacc ataaaaaaaa aagaaatgca ataaaaataa
    27661 ataagaagct cattaagatt tttctaaatt attcctcatt ataaaatttc aagtgttttt
    27721 ttattttgag ttttactctc ccgtaagcgc tcattacttg cttgtgatcc attgctaaac
    27781 attcagccat gtctacaaaa tcaacgcctt gcatataata atgaaaaatt ttagcttcta
    27841 tttttgaaaa ctggatttcg caattttcga ttgcttcttt taataaaaga tgctgaatcg
    27901 ctaaaggttc ttgcaatgtg atctctgcta aaaagtctgg tccttctgtc tctatttttt
    27961 ggtcgattga gacttcatct ttttgatcac gacgcttcag cgcgttctgc ttcctcaaca
    28021 gagaatagat ttgattgctt ataatgcttt tataataact tccaaagcta acacctttgc
    28081 ttttttcgta agttctcaat gcctgatata aagcgatcct tgcttcttgt tcccaatcct
    28141 cagaaacata atttcttaaa taataggtgt ttttcaaacg tagtaaaagt ggaaaatatt
    28201 tttgatacag ggcagcaaat gcttcttcgt caccattacg tgcagcgcgc aataactcct
    28261 cagacataaa ataattcctc cataaattcg tttatggagg aatcttatca tcaatgattc
    28321 atcgaactga ttagaacgat atgttattta ttttttctca ttaaatcgtc caatttttgg
    28381 gacaattttt ttaactgctc ttcattccat ggagaattac gtcggaaatc aataaaatgc
    28441 atatctttat tttcttgggc tattgttttt tccgtttttt ttatcgtctt atataattca
    28501 taagcagaag ttcgtaatgc tccttgggaa aagatcatcc actgttctgc taaatcgctt
    28561 gtcgccactg tcacttgtgt caaacggtca ttcagctgac cagcgattct ctcgatataa
    28621 ctgtcagccg tttcgtcttc tttcgtaaaa acaacatcca actggtattt cttataattt
    28681 tgttggatgc ccggaactag ttgggcatcg aagacaacga tgacttccat tccttcatat
    28741 ttcgcataat ttgataaacg atgtaaaaga gcttctcttg catccgccat tttgtcctgc
    28801 tttttaagct ggacaagttc cggccacgaa ccaatcatgt tgtagccgtc tacaatcaac
    28861 agctgttttt tcatggtcta ctccccctga cgttgggaaa aagcctgata cataagcaaa
    28921 ccagcagcaa cactggcatt taagctctgg acgtgcccgg tcatcgggat cgtcagcaat
    28981 tcatccattt cttttttcaa tcctggactc attcctttgc cttcattacc aatgactaaa
    29041 gcaattgatc ctttggcatt ccatttttgg aagtttgtgc cggtcatatc tgtgccaaat
    29101 acccaaaatc ctgtttcttt gagctgttgc acagcttgta ccaaattcgt cacgcgtgca
    29161 acagggacat gttcgactgc cccagtggaa gtctttgtta cgataggtgt gattccaact
    29221 gctcggtgtt ttggtatgat cacaccatcg acgccacttg cgtctgctgt ccgtaaaatc
    29281 gacccaaaat tgtgtggatc ttcgatgcta tccaaaatca aaaagaaggg gtcttctttt
    29341 ttctcttttg ttcgttcaat cagttggtca agggataaat attcataagg tgtaatcgca
    29401 agaacgactc cttgatgcac gccgtgatcg ctcaaagttt ctaatttttg tttaggaacc
    29461 catttgactg gcacagcttg ttcacgtgcg atttctttca gttcttctac tttttctcct
    29521 cgtgaatctt cttgaagaaa gagtttattc cctcgccctt gttgtagcgc ttctactgcg
    29581 gcatggtgcc caaagacgaa attatcttct aatgcttctt cattttgatc agaaactgac
    29641 tgatttcgtc cagtttgctt tttatttgaa tatctatttt gttttttgtc ttcaaatcca
    29701 cgtttcgatt gatgtttgtt tgttctttct ttacccattt ttttcaccaa ctttctgaat
    29761 acaccagcgg atcaattctt ccatccgctc ggtttgtttt gtcaaatgca gatagcccat
    29821 gagcgcttcg aaccctgttg cgacccgata cgtcgtaaca tcagcatttt ttgcgctcgt
    29881 atggctttta gcatttcttc ctctgcgata ataaagttct tcttcttctg tcaaaagatg
    29941 ttcttccagc atttgctgaa tcaaaaaggc ttgtgcattc gcagaaacat aattcgttgc
    30001 cgcacgatgc agcttattgg gttttgtttc tccttgatca atcaggtggt cacgaacata
    30061 gatttcgtaa atcgcatctc caacataagc caaggctaaa ccgttcaatt gtgtgtaatc
    30121 tctcatgctt ctcttctcca tcgggttcct tgcggtgtat cttctaataa gatccctttt
    30181 tctttcaaca aatcacgaat ttcgtcgctt cggacaaaat cacgattgct tcttgcttga
    30241 tttcgttctt cgatcaattg ttcaatctct tcatctaata actcttctgc tgtaaataaa
    30301 ataccgaaga tcgaaagcca ttgcgagaat aaagcttctc cttgtgccaa gacagcggca
    30361 gaaactttcg gctggtctag ataagtgttc agccacttag caagctcata gaccactgtg
    30421 atcccatttg ctgcattaaa atcatcgtcc atttcttcga tgaatcgctg ttctaaattt
    30481 tctaacaaag cgattttatc cgcatcatct gccaaattct ctgaggcagt ttcttggcgg
    30541 aatttcaaat tgtcaaacgt attcttcagt cgttgtaaat tggtggctgc ttctttcatc
    30601 gtcgtttcac tgtagcggat cggacgacgg tattgtgtgg tcgccatgaa gaaacgcaag
    30661 acttgtggat caacgttttt gatcatttca tgtacagtga tgaaattccc tagagattta
    30721 ctcatttttt catcgtcttc gccaatcgtt acatagccat tgtgcatcca gtaattagcg
    30781 aattttttcc ctgttttcgc ttcactttgt gcgatttcat tttcatgatg aggaaattct
    30841 aaatcttgcc cgccaccatg gatatcgatc gtttctccta aatgttttgt tgccataacg
    30901 gaacattcaa tatgccaacc gggacgtcct ttgccccatg gtgaatccca agaaatctcg
    30961 ccaggttttg cttgtttcca taaagcgaaa tctaatgggt cttctttttg ctgctgttct
    31021 tcccctgtac gctgacttgc tccgacttcc agttcatcga tggattggtc acttaatttc
    31081 ccataatttt caaatttacg ggtacggtaa tacacatcac ctgaagattc ataagcatag
    31141 cctttttcaa ccaacacttg aataaatgct aaaatatctg gcatatgatc catgactctt
    31201 ggatgcagtg tagcaggctc gacattcaac gctttcgtat cttcttcaaa tgctcggatg
    31261 aaacgatcag caacttctgg tgctgtaata cctaattcgt ttgctgtacg gatgatttta
    31321 tcgtctacat ccgtgaaatt cgatacgaaa tcgactttat agcctcgata ttcaaaatag
    31381 cgtcgaattg tatcgaaagc aatggtgcta cgagcatttc cgatatggat ataattatac
    31441 acagtcggtc cgcagacata cataccgaca tgtccctttt gtatagggat gaattcttct
    31501 ttttttctgg tcaatgtatt gtagattttt atcattctga tctcctcact ttttctcctt
    31561 tgattcgaac gacttttgct ggtactccta ccgctgtagc gtcgtctgga acatctttca
    31621 acacaacggc acctgcaccg attttggcgt tttttccgat cgttaccggt cctaatatct
    31681 gcgcattcgc agagagcatg gctccttgtt taacagttgg gtgtctttta cccgtttctt
    31741 ttcctgtgcc gcctaatgtt acgccatgga ataaaatcac atcgtcctcg atttctgcgg
    31801 tttcgccaat gacgattccc attccatggt cgatgaacac gccagttcca atcgttgctc
    31861 ctggatggat ctcaacacca gttaggaaac gccagaattg agcattgatc ttcgccaata
    31921 aaaagagccg gtgctgatat aaaaaatgag agaaacgatg ccaaaacaag gcatgaaaac
    31981 cagggtatgt caaaacgatc tcagctgtcg aacgtgcagc cggatcgttt ctctttgctg
    32041 catcaatcgc tcgggtaaac caactcatga ctttccactc ctttcgtaag aattttatgt
    32101 aggaaatcat cttcttataa agaaaagaag cgccttttaa ataagtttcc ttatcaaaag
    32161 acgctttccg cgtggttcca cttttcttca taatcaatga ttatctctga cagctaacgc
    32221 ctgttatacg ttcttggcta ctttgtttcc tataaaacag tttttcctgt tttctgtgtt
    32281 tcgccaagac cactcccaga ggcatttcgg caaccattca ttgatacctt gcaccacccg
    32341 gtatctctct tgtaatgatc agctgcttac ttttttctgt tcatcgtgtt tagttcagta
    32401 cttgatttaa atgtgctaat gatttttcac gaccaagcaa ttcgatcgtt tcaggcaatt
    32461 ctggaccatg catttgtcct gatacagcta cacggattgg catgaaaaga ttttttcctt
    32521 ttacgccagt ttctttttga acagctttga tggcagcttt gacagttgga gcatctacta
    32581 cttccatttg ttctagctgt gctttgaatg cgtggagaac agtaggaact gtttcaccag
    32641 caagtacttc ttttgctgct tcattcaata caggatgttc attgaaaaat agcgtagaca
    32701 gatcaacgat ttctgcagca tagctcattt gaggttgata caggctaacg atcttcttca
    32761 cccattcgat tgtcttttca tctgggtttt ctgctacacg tccatcagcg attagataag
    32821 gcagacacat gtcagtaaat gcatctagat ccattgcttt gatgtaatgg ttgtttaccc
    32881 attgcagttt tttcgcatcg aaagctgcgg gtgatttact taggcgtttt ggatcgaaca
    32941 ttttgatcaa ttcttggtgt tcgaagattt cgtcttcgcc cacaggtgac cagcccaata
    33001 atgcaataaa gttgaacatt gcatctggta gatagcctaa ttcgcgatat tgttcgataa
    33061 attgcaagat tgattcgtca cgtttgctta gttttttacc tgtttcactg ttgatgatca
    33121 atgtcatatg tccgaaggtt ggtggtgtcc agccaaatgc ttcatagatc atcagttgtt
    33181 ttggtgtatt ggcaatatgg tcatctccac gtaaaacgtg agtgattttc atcaaatgat
    33241 catcgaccgc aacagcaaaa ttataagttg gcataccatc gcgtttttgt atgacaaagt
    33301 cgccaccgat attatcggat tcgaacgaaa tagcgccttt caccatatct gtaaatgcgt
    33361 attctgtatt gcgaggcaca cggaaacgta caacagattc tagaccttgt gcttcttttt
    33421 ctgcttgttc ttcaggtgtc aagttcgcac atgttcctgc gtaatgaggc atttctcctc
    33481 ttgcgcgttg tgcttcccgt tctgcttcta attcttcttc tgtgcaatag catttgtatg
    33541 ctcgattgct agcaagcagc tgatcaatca gcggttgata gatttcttta cgttcagatt
    33601 gacggtaagg gccgtattct ccaggatttt caggagattc atcccaattc attcccaacc
    33661 aagcaaggtt ttccaactgg cttttttcgc catcttcgat attacgtttt tggtcagtat
    33721 cctcaatacg aataataaag tctccatcat tgtggcgggc aaacaaataa ttgaataatg
    33781 cagttcttgc atttccaata tgcaggtgcc cagttggact tggtgcatag cgtacgcgta
    33841 ctttcgtcat tttcttcctc ttctttcaaa ctagactttg actaacagca aaaattccgt
    33901 cagttacggc ttctcaaata tcatttattt aaagccactc agcacaaatt ttaccttttc
    33961 taaggtaaat agtaaagcca actggttgaa tttccttacc agttggctga atttcttttt
    34021 tattttgatt gtttagtctt tttctctgtc tcttttttat tgtcgattcc tcgtcctgca
    34081 tgtgcaggtt tagcaaagat catgcgtcca gcagctgttt gaagggcact tgttacaacg
    34141 acttcgatac gttcattcat gtaatgctgt ccatcttcta cgacaaccat cgtgccgtca
    34201 tcaaggtagg ctactccttg ttggcgttct gtcccggctt ttactactag aacattcatc
    34261 gtttcaccag ggatgacgac tggttttacg gcatttgcca acgcattgat attaagaaca
    34321 ggtacatttt ggaattcaga gactttattc aagttatagt cattggttac aatgacacca
    34381 tctaaaagct tagccaattt gatcaatttg ctgtctactt cactgatatc ttcaaagtct
    34441 ccatcgtaca tttcgacaga tataccctct tctttttgca aggcatttag gatatctaat
    34501 ccgcgacgac cacgcacacg tttcaaacta tcccctgagt ccgcgatgta ttgtagttcg
    34561 taaagaacaa aattagggat catcaaggtt ccttctaaga atccagtctt tgtaatatca
    34621 taaatccgac cgtcaatgat cacgcttgta tccaagattt tatatttatg gaaattttct
    34681 cctgattttc gttctaacag ttgactctca acttgactag ctgtttcttg ctcgattttt
    34741 ttgcttcgtg aaccaaaaac tttcttccat tcttcgattc gtgtcgttcc cattctcaat
    34801 cccagatagc ctaagatgat catcagtaaa atcggcaata cactattgac gaatggaatt
    34861 tccatattgt aaagaggggt cgagatcaca acacctaaag tcaatccgat gatcgcaccg
    34921 atcgcaccga agagaagata agtcaagctg acctcactta gtttttgttc tacttgtttg
    34981 ataatagctg agatatgttt ggccaacagc aaagataata aaaagaaaat aagtgcgcct
    35041 aacaggctgt tggtaaatgt attattcagc catccgtttg actgttgacc gattgcctgc
    35101 caagctgagg gtaaaaatga aatacccaag cttgcgccga caatgatcat cagcaaagta
    35161 atgacacgtt tttgcatcgt ttttcctccg tttcgacaat catttattag ttagcggaac
    35221 acacgcttca acgtttcagc caaagttgct actccaacta tctcgattcc ttttggcgct
    35281 tcccagttac ctaagttgtt tttaggtagg tatacttttg tgaagccgag tttttggact
    35341 tctttgactc gttgttcaat atgactgaca cgtctgattt ctccagtcag tccaatttca
    35401 ccgatgaaac attccgttgg ctgtgttcct ttttctttat agcttgaagc aatactgaca
    35461 gcgatagcta aatcaatcgc tggttcattt aattttaccc cacctgcagc ttttaagtaa
    35521 gcatcttgat tctgaagaag caacccagct cttttttcca gcactgccat gatcagagat
    35581 acccgattga aatcaagacc tgttgttgtt cgtttggcat ttccgaacat agtaggtgtg
    35641 actaatgctt ggatctctac caaaattgga cgtgtccctt ccattgccac aacgatagca
    35701 gagccagttg caccgtctag gcgttcttct aagaaaatct gtgaaggatt agcgacttct
    35761 tccaatccat gctcatgcat ttcaaaaatc ccaatctcat tcgttgagcc aaatcggttt
    35821 tttactgctc gcaaaatccg aaaactatga tgttgttcac cttcaaaata taacaccgta
    35881 tccaccatat gttctaacat tcttgggccg gcgatcgagc cttctttggt aacatgaccg
    35941 acgataaaaa tggctatccc atttgtttta gcgattttga gtagttctgc agtggtttct
    36001 cttacttggc tgacacttcc tgccacactt gtgatgtctg gctgcgtcat cgtttggatg
    36061 gaatcaatga taacataatc aggggagata tgttcgatgg cacgactgat ttcgttcata
    36121 tctgtttcag cgtatagata aaattctgtg ttgatacttc cgaggcgttc cgcgcgtaat
    36181 tttatctgct ctgcgctttc ttcgccggat acatataaga cttttccgcc tatggctgct
    36241 aattgctgag aaacttgtaa cagcaaagtg gatttcccga tacctggatc gccaccaatc
    36301 aagaccatag accctggcac cacacctccg cctaacacac ggttcagttc ttctaatttt
    36361 gttttgatcc gaggttcttt tttagggacg acgtctgcga tttttgttgg ttttgctttt
    36421 tctcctgtca aacttgtccg agttctgcgg tccgttgtat cttgttcgat ttcttcgacc
    36481 atcgtattcc attttccgca atttgggcag cgtcccaaga acttaggcga cacatacccg
    36541 caattttggc aaacaaattg tacttttgat ttttttgcca ttgtaccgcc tccttttatc
    36601 ctaatcatga gacattaaat caagcttaag tatctttaac gttttattta ctggaagaac
    36661 caaatccgcc ttgacgttga gcagtcgttt gatcttgatc tgcttttaaa aacggcaaaa
    36721 agatcccttg gccgatacgc tcgccttttt tgatcactac atccttgaaa ccaaagttgg
    36781 tgaattggaa catgatatgg ccttcatttg cctcattatt gtaataatca ctgtcaatca
    36841 cacctactcc gtttgtcaac actaaaaacc gcttcaacgg attgcttgaa cggttagcaa
    36901 gctgcaaaaa ttcatcttct cccatgtaag ctttgattcc agtaggcacg agggttggtt
    36961 tcaattgttt ttcattttct gatacatctt ttttcaatcc atctgcgact gtttccaacc
    37021 cctttttcca gcctgctttc cagatacttg gaatcacgac ctcttctgct gcttcaaaat
    37081 catagccagc agcatgatgt gtagcgcgct gcggtagatt gatattttga tctttatatt
    37141 tagaaataac ttcaaatcct ctttttctca ccggttttcc tccctaaata aactactcct
    37201 attctaacat atccgaggct gtaaaagact ttctttacaa attcttcaag aaattctgct
    37261 atcttaaagt aaaccattaa ttcgttcttt ttccaaacga ctgcaagttg tacaaaaaaa
    37321 ctatttttta catgacttta ctcgtcaaaa caagtagaat agaacgaaat taatgagtgg
    37381 atatttgatt gaggtgtcac atgcgtaaag aaattaaaaa tgcaaaacga attattgtca
    37441 aagtaggaac aagtacattg atttatccca atggaaatat caatcttgcg gcaatcgatc
    37501 agttagcttt tgtcctttct gatcttagaa atcggggaaa ggaagtgatc ttagtctcat
    37561 caggagctgt aggcgttggt atgcataaat tacatttgtc agaacgtccg actttgattc
    37621 ctgaacagca agctgcggcg gctgttggtc aagccgaact gatgaatatc tataatcaaa
    37681 gattcagtgt atataatcaa cagacggcac agatcctcgt gacaagagac gtggtagatt
    37741 atcctgaaag tcgtaaaaat gtgaggaata cattggaaca gcttttgatt atggacacga
    37801 tccctatcgt gaacgaaaac gatactgtag cagtggatga attagatcat ctgacaaaat
    37861 ttggcgataa tgatcagctg tcagccatca tggcaaaact tgtcgcggca gaccttttga
    37921 tcgtcctttc tgatatcgac gggttttatt cagataatcc gactacaaat cccgaggctc
    37981 agatgtatcg ctatatcgat aaaatcgatg aaaagattct ggcacaagca ggtggtgctg
    38041 gtagcacatt tggaacaggc ggtatgcaga gtaaattaaa agccgcttct aggatatttg
    38101 aagcaaacag cgcaatgatt ttagcaaatg gaaaaaatcc ggcaattatt tttgatattc
    38161 ttgcaggaaa agaagtaggt actttattta aggaggactt gaaatgactg acttgatgca
    38221 attaggacaa caggcaaaag atagcgcgca acaactagca cttatgccta caaaccgcaa
    38281 aaatgagcta cttgttcaaa tggcacaaac catcaaaaag aatcaacaag cgatcattga
    38341 agcaaatcat aaagacttag aaaaagcagt cgaaaacaat atcagtgaaa cgatgcaaga
    38401 ccgcttgcag ctgacacctg agcgaatcaa tgccatggct acagagatcg aaaaaattgc
    38461 ttcgttagac gatcctattg gaaaagtaga tgagatgtgg acgaacaccg acggattgag
    38521 aatcggcaaa aagcgcgtgc cactaggcgt gaccggcatc atttacgaat ctcgtccaaa
    38581 tgtcacgaca gatgcagcta gtctagcatt caaatcaggg aatgccgtta tccttcgcgg
    38641 tggcaaagaa gcattcttct cgaatcaatt attagtccaa ttattgcagc aagtactggt
    38701 cagtgctggc gaatctcctt atgccatcca gtttgtcgat gatacttctc atgaaactgc
    38761 aggacagttg atgcgattga cagaatactt agatgtgctg attcctcgag gcggagcaaa
    38821 attaatccaa cgagtcaaag aacaggcaac ggtacctatc attgaaacag gtacaggaaa
    38881 taatcacgta tatatcgata aagaagcaca gttatctatg gcagtgaaca tcatcgtcaa
    38941 tgcaaaagct tcccgtccat ctgtctgtaa cgcggcagaa acattgctga tccattctga
    39001 gatcgcacct tttttcttgc cagctattga aaaagaactg gtagaacacg gggtctcttt
    39061 acgtgcggat gctcgtgcgt tagaatatct agagacggct gctttagcag aagacacaga
    39121 ttgggatact gaatatctag attatatcct tgctgtcaaa gtggtcgatt ctttggatga
    39181 agcaattgca catatcaatc ggcataatac taaacattca gaaacgatcg tgacagatag
    39241 ttatgcggct agtcaacgtt tcttaaatga agtggacgcg gcggctgtct atgtcaatgc
    39301 ctccactcgt tttacagacg gatcggtatt tggttttggt gcagagattg gtatctctac
    39361 tcaaaagttg catgcccgtg gtccaatggg gttaaatgaa ttaacttcca caaaatatat
    39421 catttatggc gatggacaaa tccgcgaata atgtttcgtg tgaaacatta ttcccgcctt
    39481 ttgccttttt tctgtaaagg ttctatccaa ctagaaaggt gtcaatatgt ttgaaatttt
    39541 aatgattttt tttatttacc ttatttctct gaatatcgct gcttttctgg gagtcggtat
    39601 tttatccctt ttctttcaat tcaaaaaacg aagcataggg agtcaacggg aaaaatggtc
    39661 gcagtatttt gacaaaatcg ggcaaaaagg gctggttaca cgcttgcaca ttagttacat
    39721 ggttgcatta tgcttattag cgacaatcaa ttactattct tttttcgatc attcaatcgc
    39781 ttatacgatt acgctactga ttgctgggat ttttcactta tcttataaat atcagctgaa
    39841 taaaaaccac ttgaatcgga ctttccgcta aaaaatatcc gtacgataag tagagtgcct
    39901 tgaagatcgg caaaaaactg ccggactttt tagaggctgt gacaaagatt ttgtcacagc
    39961 ctctttttcc atataaacgg cgttcgccta ccagctgcct ggtaaatctg tttcctcttc
    40021 gttaagaagc gaaaatccgt caaatgtttc gccatcataa taacgaagca gctcatccat
    40081 ggacaagttc atcgtttttt ctttttcttc ttccgctgat tcagccagta gctgttcccg
    40141 atacacctga tacataccag ctattttttg ctgcatcgct tttctagttt caccttccgc
    40201 attgattatt ggcaattctt tgatatagcc acgataaatc aaccgatcat ctgaaaattc
    40261 aatgggtaat tcaacgagtg tcaacccgac ttcctgaaaa tattcttcca gcatctatta
    40321 tccccccatt ctttttctta aatgaatttt agcttaccta ctgaaaattt tccagtttca
    40381 ttattttgag aaaaaaaggg aatgtgacaa aaatcgtgtc acatcccctt tttccgaata
    40441 aatggtgttc ttctgccaac tcctcaatca taagtcacaa tattcaacaa acatgagaaa
    40501 ctgttttttg aaattgttcc ttgtgattca gagttaaacg acagtctcac aacctcttgg
    40561 taaacggcgt tcaaaagctt gcatctgcgg taataagccc aaccaaacga aaaatatgag
    40621 aagctatttt tgtttggctg ttcttatgac ttgatgcagg acagcttttt cacaacctct
    40681 gatttcttat tcttcttcat ttgtagcaaa ataattacgg tgataaatga tacaacatac
    40741 actgataaga acaatggcac ctgcaaaata aaacgcatag ctttcaccaa tatgttcacc
    40801 aatcacacct gcaataaatg gtgctaaagc tccaccgaaa aagcggacaa aactatagct
    40861 ggacgaagca acgtttcgtt gcagacctgg tatttccata gcaatcgtcg tcaacagtgt
    40921 attgataatt ccttggaaaa atcctgctaa cacaatgctg atcggtacaa ggactggcat
    40981 agaagttcca gctcctaata acagcaaaca aataatgaat aagacaaatg ctgctaaaat
    41041 tgtccgataa gtcgtaaact tccgctcaag agccggtgca acgaaaatcg aagcaatagc
    41101 cagaagcaca ccccagccaa aaaatacaaa accgacttct aattcagaat agccaatcaa
    41161 taaaaatggc gcatacgcaa gtaaagtaaa gaatccgaaa ttatataaca atgcgattag
    41221 tccaactgaa cgaagtttat gattgcttaa cgctttgaca ccttcccaaa ggcctacttt
    41281 tctctttggt ttctcagttt caggtaaaag taccgctagg gctataaaac cgcaaaacat
    41341 caaaaatgct accccaaaaa atggataacg ccatgaacca ctgcccaaga ccccaccgac
    41401 tagagggcca acagccattc cgcagccaag tgctgcttca tacatcatga ttgctttttc
    41461 tgtgttacca actaaaatac tgacgatcgt tgctaaagca gtagagacaa ataaagcatt
    41521 cccgacaccc cagccggcac gtaacccaat cagcattccg atattcccag ataatccgcc
    41581 aagaccagcg aatacaacga tgataaacaa tcctgtcatc aatgtttttt caaaccgatc
    41641 cgactagata agaaacctgt aaatagcatc accacaccag tgactaacat gtaactagta
    41701 aataacaggg tcgtttcagc tggcgtagcg tttaactgtg cagcaatcgt ttttaagatt
    41761 gggtctacta gacccactcc cataaaagcg atcatacagg tagctgctac aactagcgct
    41821 gaacttttcc cctttgtttc ttcatttgtt tgttcttgta tatctatttg attctccaat
    41881 atttccatcc ctttctctct attagcatca tacatgattt attttacatg taaatatgca
    41941 catgtaaaac gtggaaactt tactgtgata taataaagaa aataagacga gaggtaggat
    42001 ttttatgaat actttagcct atatattatt atgcgtgctt ttatccaaac cacgttctgg
    42061 gtatgaattg aagcagctga tcacaatttt ttgggaagcc catcacagtc agatctatac
    42121 aacgcttgcc aagttagacc agcagggtta tgtgaccgtt ttggacaaag acgagcactc
    42181 tcaaaaaaag atctaccact tgactgatga aggacgtgct cttgtagagg aatggatcaa
    42241 agaggaaact cccgctccta cgcaaaaaga tgaatttcta gcaaaaatct atgctttttc
    42301 gacgctcgat aaaacaacag ctactggact gctattcact agagaacaac agctgaacaa
    42361 aatcatgcaa aaaaatcaaa aaaaattgaa tgacctctcg ctagacaaga aacaagaatt
    42421 tggacgttat gttgtgatcc agcgcagaat tatgctttgt cagcaggaac tccaatggtg
    42481 tcagcaagta agagaacaaa tgcagcagtt ttttgagtaa gggataaaca gaacctttct
    42541 agaaatatct attgaagagc aaattatctt caataaaaga gcaatacagt tggaaaagaa
    42601 aaagcgatcc gaacgacagg ccaaaaccta tcgttcggat cactattttt ctagaactgc
    42661 attcaagaga ctatgacaaa aagcatgcca aggccttttt tcaaataaca gtgttccatc
    42721 attcattact ttgaattgct tttagcacac ttcttgaaag cgggccaacg atgatcaact
    42781 gcaacggtaa agcggcaatc acattcattt tccaagcatt catataagca gagaataatt
    42841 gatcaggtat tccttgttcc atgatcacac cgaataaaga catacatgtc accattccta
    42901 aaaccatcaa gcatgaaata gttaagatca tttggacctt gctttctgga tttactggta
    42961 gtttaaaggc aatttttttc gccagcacac cgatgatgaa gacatccaaa atgaaagcca
    43021 ccacgaatcc aggcactaga ccaatcaaca aacttgataa atgaagatct tgatgcagcc
    43081 ataaattata cgcgctcatc cccaagacca tcaaaaaaca catcattgtt gtaaaaatta
    43141 ttccttcttt tttatttgta ggcaatctgt accactcctt taaatttgtc acgaaaatca
    43201 tcgtagcaca tttaaaaatt tctcgtaagt acttttttta ttttctgccc ttttatttca
    43261 taaacttcat cagccgtacg ctcgacaaag taagaatcat gagaagtaaa caaaatcgtt
    43321 cccggatata tttgcattag cttctctaaa gcttcgatcg ttgccatatc aataaaatta
    43381 gtcggttcat ctaacagcaa cacattactt ggcttagtaa aaagcaaagc aatcgtcaga
    43441 cgggtcgctt ctcccccact taatgtacat aacggtttcg tcaaagtttc ctgagcaaac
    43501 cctaagtgat tcagcaaact acggacaagc gattctgaaa actctgtttg tctcatgaca
    43561 aaacgaatga tggattcttc agatgtcatt tgataaccct tttgctgata tacttgaaag
    43621 cttactttcg gagagagaag gattccttct ccttgtttgc ggatatgttc taataaggtt
    43681 gtcttacctg aaccattttc accgacaatc gcgatttttt tacctaaaga aaactgaaaa
    43741 tcaccattta ccagtaatgt tctcccactt ctttccaatt ggacattttg tcccatgatg
    43801 ggatagcggc tgtggagttc aagagatttt ggtactggaa aacggatttg tttcatctgc
    43861 tgcggcttgg ttgtttctcc tattttttgg agtcttctct ctaatgtttt cgcattcttt
    43921 tggatggctt tttgtacact gtctttttgt ttagaggaag acaaccgatc cggtctgatt
    43981 tgttgctttt tctttttact ggacactttt tccaactttt ccgcttgttt tttcttttct
    44041 tggatcgatt tttccaatcg ttttttctct ttcatgatcg actctgcttc tcgtagctga
    44101 gtctttttct ccaattcctt ttgagtgaga taggcactat aattccccga aaactctcgg
    44161 atttctccgt cttttacttc ccagattttc gatgctagct catctagaaa atagcgatca
    44221 tgactaacaa aaaccagcgt accataataa taacgaagct cttcaatcag tcgatcaata
    44281 ctttttctat ctagatgggt tgttggctcg tctagaagca tcccttgttc ataagaagaa
    44341 agaagacgta tcaactcgat ctttcgttct tcccctccgc tccatttccg ctctgaagtt
    44401 tcttccagtc ttaactggaa atgactggct aattccaaat ttaggtcctt tgcttcagcg
    44461 agataggtta attgaggcag gtagttgaag gtgatctctg tttggatttt cccatgatcg
    44521 ggaaaaagtt caccggctat tagtttgagc aaagtcgatt taccgacacc attttctcct
    44581 atgatcccta tacgatcttg ctgatagaca cgaagagaat caatagatag ttctagctgg
    44641 ttgccaaagc tgacttgtag atttgttata tttactgcta aattttccat atgttttaca
    44701 ctcctatttc atgtaaaaca acaattatcg atactaaaaa agagcagaaa aatctgtttg
    44761 caaaaaaact gcatcacatc ttttcctact ctgaacgaaa taactatgac taaatcacca
    44821 aatctgtcat agctttcacc tattctactg tcgttcgaaa aaatggacat actccatcca
    44881 tgaaattgtt gtttatacat gtcttttgaa tttatttagt tgttattcaa aagttcgaaa
    44941 cgtaatttca tcgatgcagt catttttccg acctcctttt ttattaggtt accacacacg
    45001 attcaaaaaa agcaatagct actgaatagt aactgaaaac cttttattcg aacgtagtga
    45061 tcttttctgg tgctaaacga acagactcgt aaccttcttc taccgctttt cctattgata
    45121 aaataataac tggaacataa cggtctttat ccaaaccaaa tgcctctgcc aactgatctg
    45181 cttcgaatcc accaattgga tttgtttcat agccatgatc acgcgcaact aacatgaatt
    45241 gcatagccgc taagctagaa tcgactttga ctacatcgtt catttcttct cttgaaaaac
    45301 tcttgtaata aggaatgatc gcagctaatt gttgatctct gacttctttt ggcatcttcc
    45361 ctgattcgta agcttggtta tagatttctt cgccatattc gtaacattgc atatcgccaa
    45421 aaatcatgac cattgcagaa gaagtatcat tttgtctcgt attgaaacgg atcaaaggac
    45481 gcaagatttc ttttgcttcc tcgctttcag cgaccacaaa gcgccatggt tgcatattga
    45541 cagaagaagg tgcagtcgtc gcttctttga tcatttccag catttcttcg tgtgggattt
    45601 tgtatgtttc atcatatacg cgaacggatc ttctaccagt cataattgtt gaaaaatcat
    45661 ttttttgttc cattattatt aacctctttc gtttctgttg ttatattgca aagttgggta
    45721 ttcaatttat ctaaaagtgt tagaaattgt ttcatttcta catcgctgaa agccggggat
    45781 agcacggaac ctgtatcttt atggtccatt tcacaatgct cgattttctt ctgggctttt
    45841 tctgttagcg ctacacacac ttcccggttg ttctgaggat ttctctgacg cgaaacatag
    45901 ccatttgctt ccaatacctt cagatgacga gtaactgctc cttgatcaat ctgcaacttt
    45961 tcttgcaaac tgctttgtag caatggattt tcttctttca gtctcatcat gatctcgtat
    46021 ctagtaagac ttataccgat attttcttca aagatctgga cgattttttg atcggctaat
    46081 ttgatttgat ataaaaggtg actaatctct tctaaagaca taatttcctc ctttctaata
    46141 attgactcgt caacgattga cttgtcaata atagaataaa aaaagaaaaa agtcaaagaa
    46201 tcagctgtgt aataaaaaaa acgctctcaa tagaaaagta tagagaaaag aaattttttg
    46261 tagaatgaac ggaaaaaatg ataaagaaag cacaacgcta aaaccaagat tattgattct
    46321 tatcaaacta tagaagatag ctcttctgca gtagaaaatt ttacaccgcc aaaaatttcc
    46381 gaaatcgcct tttgcaagaa tcccgtcgaa tattagaaat tggatcagta tgcttaaaca
    46441 gtaataaaaa agttatgaca agatgttgtc acaacctttt ttgagtaaac ggtgttcaaa
    46501 aacttgcatc tgcggtaata agttcaacca aacaaaaaac atgaaaatct gtttttgttt
    46561 ggctgttctt actacttgat gcagggcagc tttttcacta cctcttttta ataaccataa
    46621 aatcctattc cttcatatct ccagcccatc ggtgttagcc aatctctttc cttagcactc
    46681 aaggtatagt gatgggtacc agttttttca tttggattaa atagtcgata aattggcttg
    46741 gaaccaccag aataaaaact tagaccttca taacgccaac cgatttttgt taaagagttg
    46801 atctcagatt gtgccattgt gtaatgatgg tctccactgt aaggattgta taaacggtag
    46861 acaggttgtc cgctgtttgg tgcttgccaa gcaatccctt catattccca gcctacagtt
    46921 ggcagataat ttttttctcc ggcattcaat gtatataaat gttctcctgt atgacggtta
    46981 tacagacgat acatcggaga agttgaagtt ggtgctgtat catactgtgt cagattatag
    47041 gtttcgatta cattgttcaa tgtagaacca taattaggcg cagtggcata acgacctgtt
    47101 agccaagccg tagcatcttt gtaagagttc gtattgcttt tccatgcacc ggaatagtag
    47161 taaacgcctg gctgaaaaga agtcgtcttc aataccgtta cgttatcttg caatgattct
    47221 ttgtacgaag gatattttct aaaagcggca ttcaccgtaa tccattgccc attcacatat
    47281 tccgaagtag gcatcgtaac agattggcca ttatagcttc ctttaatccc aaatagattg
    47341 taattagggg cagaagccaa agtactgttc ccccagccac tttcaacgat ggcttgagca
    47401 atcatgactg atgcatataa gtcattggca cttgccaaac tttgtgcagt tgaggcaata
    47461 ctatcaatga acgcttgttg agaattagca gatgagcgag cataaaaagc atagccagta
    47521 tagttttcga attcagacaa atctgcttga tgggtatgtg tcaatgtttg cccatctgtc
    47581 aattggttcg tgatttcttc tgcattctct ggattgttct gagcttctgc tgatgtactg
    47641 tcttcggttt gtgtttgttc tgttgtttca gttgttggtt cttcggttga aattgtattt
    47701 tctgagacag tagctgactg caaagtactc tctgtatcgt tttcaattcc tgtttctgaa
    47761 gaactcgatg tctgagttgc gacagtttct gtcgattctt cagctagcac tgttgtgttg
    47821 gcaactgcta gatttcctaa aagaataacg ctaaataata aattctttgc tttcatcttt
    47881 tttctccttt gaattatgaa acttttccta aataacagtg taactaaaaa agtaaaagca
    47941 agtcaatgtc catatttaaa taacatttgt ttctttttga taaatttgct ttttctacat
    48001 aaaaagacgt aaatgaagaa tattcttctc cattcacgcc ttaaattgat ggtcgtttga
    48061 ataactattt aacaagtaat tctctttgga tattatccgt tatggattat acttcctgtg
    48121 tcgtcggacg gataatcatg ttactgacag acatccggtc tggcgtttct atcgcaaatg
    48181 ctacagcaga agctatatct tcggaggtca atccccattc tttttgcgct tcatgaagtt
    48241 caagagcagc ctttttgtcg ctgatggttt gatataattc agtttgaaca gctcctggag
    48301 agatgatcgt agatttgatg ttattttgcc gttgctcttg acgtaatcct tccatgattg
    48361 cacggacagc aaattttgtt ccgcaataaa cggcggaatc aggataaaca acatgtcccg
    48421 ccacagaatc agttgaaatg atatgcccag atttttgttt ttccatgatt ggaagtacgg
    48481 cggaaattcc atttaacaca cccatgatgt tgatatcaag catattttgc cattcatcac
    48541 ggtgaccttc aactaatgga gcggttggca tgattcctgc attattgtac aatacatcga
    48601 ttcgaccgaa cttttccatc gctaaatcga taactgcttg gacttgtgca aaatctctta
    48661 catctgctgt ttgaatatat agctcagctt ctggtaaaga ttcttttatt gcttttaggc
    48721 gatcttctcg gcgtgcagct ataactagtt tagccccttt ttctgctaat agcttcgttg
    48781 ttgcttcacc gattccgctt gaagcgccca tgatgacaat cactttttct gataaatcat
    48841 tcattctatt ctcctcctgt tttgaaaaat ctcttgctta tactcactat ctcttatttc
    48901 atcttactgt tcaaataatc cgttcgacag gtttgacgat tttggcaggg attcctgcaa
    48961 cgatggtatt gtctgggaca tctttcgtaa cagttgcatt cgctgcaaca atggcattct
    49021 cgccaatcgt tacgcctggc agaatagttg ctcctgctcc gatccaagca tttttcttga
    49081 tgtggattgg ctttactgac aagcctcttc gttcttttgg atcggttaga tggttcaccg
    49141 tgatgaggtt gacacgagga ccaatcaata ctgaatcttc taaacagatg ccacctaagt
    49201 caacgaacat cacttgacga ttgataaaga catttttccc tatactaata tgtttgccga
    49261 aatctgtata aaaaggcagc agaacagtca ccgtatcatc tactttgtca ctaatgatat
    49321 tttctagccg tttgcggata tctgctggtt catggtaccc agtattcagc gctgctaaag
    49381 gctcttcgtt ttcagcttgg atctgatgga tcacttcaaa gagcggatcc gttactcgaa
    49441 tctctttatt gacgatttgt ttttcgatga ggcttgtcat tttgctccct ccttttatcc
    49501 ttactttata acggacggct tatcatgtct aatacttata aaacatactt aactattcct
    49561 tttaactata cttagtggta tgctttttta aaaacaggag ggaaaaaatg gatattcgta
    49621 tactgacgta ttttttagca gtagctagag aaaaaaatat cagccgagca gcaaaatctc
    49681 tgcatatcac gcaacctact ctctcaaaac aactgaaaga tttagaagaa gaacttggtg
    49741 tgacactttt cactcggggc tcccgagaga tacagttgac ggattctggt cgatatcttt
    49801 attcaaaagg aaaagaaatc ttgtcgttgg tagaaaaaac ggcgaacaat ttaacaaatg
    49861 acgcaatcat ctctggagaa atctacattg gcggaggcga aactaaagca atctctttca
    49921 tttctgatag ccttcattcg ttgatcaagg cgcatcctga tatccagttt catttatata
    49981 gcgggaatgc agatgatatc atagacaaac tggatagcgg attacttgat tttggactcg
    50041 taatcgaacc gacaaacaaa caaaaatacc agtcatttcg actgccagcc caagatcgtt
    50101 ggggactttt gatacatcga aatcatcctt tagcaaagca aaccgtcatc catccgcagg
    50161 aactgaaaac tgttcctttg atcatttccc ggcaaacgtc agtggacagt caactagctg
    50221 aatggctagg tttcagcata gatcagttat cgatcgtcgg aacatataac ttattgtaca
    50281 atgcttcttt gatggtcgat aacgggactt ttggtgcatt agctctagat gggatcatca
    50341 atacttatgg gacggaaaaa atattcatcc cactcgatcc gccattacaa gcttcactga
    50401 atctcatttg gaaaaaagac cagcctcttt ctcctgctgc tcgtaaattt ttagatgtcc
    50461 taacggagaa atacagttaa gtagagtttt tttccttcgc tttcagcttt ttaacaacga
    50521 ataagataag caaaagatcc aacgattaaa taacataagt ttcttaattt atttcttcct
    50581 tttatttctg ataattccag taaattacta taattataaa aaaagaattg acaaaacata
    50641 actgtctata taaaattgat tacgttaaca ataattcgtt tcatgtgact agatcatact
    50701 aggcatgaaa aatctctgag gaacatggtt tccctaggag gtttttcgtg tctatttttg
    50761 tttggccgca atcaactggt ctagcaaacg tgaaaaccaa atcttcaaaa agggtgcgcg
    50821 catgaaaatc aaaaagatct ttaatcaaaa tgcagtattg gtagatgacg gtggacagga
    50881 aaaagtagct attggaaaag ggattggatt tgacagaaaa agaaatgatt tgatttttga
    50941 tcgtgatgtc gaacggatat ttgtcatgga accagaaggt caaatcaagt tgcaaaattt
    51001 acttaatcag attgatgaac gctttttatt tgcagcagaa acgatcattg atcatgcgga
    51061 aactgttttg atggaaaagt tgaatgaaca tgtattgatc gctttgaccg accacattgc
    51121 tttttctgcg gagaacatta ataacggtat cgtcatccgc aacaaacttt taagagaaat
    51181 cgaagtgctt tacagtgatg aattcagtat tgctcaatgg gcagtagagt atctgacaaa
    51241 agagcttggg attccatata cttatgacga agcaggatat atcgcaatcc atattcacag
    51301 cggacgaagc ggtcggaaca ataaccatcg cagtatccgt gaagtgacga ttgtatctga
    51361 catcatccat ttagtggaat cagaattgga aacagatatc cattcagaac cattttcact
    51421 gaattattca agactggtga atcatttacg actgcttcta caacgaaccc atgcgcaaca
    51481 atatgctgtt ttagacacgg aaatcgtaga aatggtcaaa cgaaaatacc cagaaagcta
    51541 taagatttct aaaaaaatcc gtgtgttgct gaccaaagaa taccagctcg cgatcacgaa
    51601 agaagaactg ggttacttgg ccatccatat cgagcgttta agaagcgcaa tcgtacaaac
    51661 aaatgttaac aatcatgagg aggaaaaaaa ttgaaagcat atctacaacg aatgggacgt
    51721 tcccttcaac ttccagttgc ggtcttacct gctgctgctt tactagtagg gatcggaaac
    51781 tggtgggcat cttatagtaa cgacattgtc gctcattttt tacaagctgg cggtaacgca
    51841 gttttaggtc aattaccttt gctgtttgcc gttgggttag cactagggat gtcagtggac
    51901 aaatcaggtg ctgctgcctt ggcaggtttg gtcgcatttg aagttccagt aaatgtttta
    51961 aaaactgatt cagtagctac tttattgaat atcaaaccag aatcagttga tccatctttt
    52021 gctcagatca gtaacgtatt tattggtatt attgccggat tgattgcggc agctctttat
    52081 aatcgtttcc acgaagcaaa attaccgatg gctttatctt tctttagcgg caagcgtcta
    52141 gtgccaatct tggctgcctt tgtcatgttg ctgatcagtg cagtgctact gctcgtatgg
    52201 cctccggttt actctgcttt agtttctttt gctaaattca ttttgagttt aggctggggt
    52261 ggtgcaggtc tttatggctt cttcaaccgt ttactgatcc ctacaggatt gcatcaagca
    52321 atgaactccg tgttctggtt tgacgtagca ggaatcaatg atattggtaa atttttggct
    52381 tctgaaggaa caaaaggcgt aacaggaatg tatcaagctg gatttttccc aatcatgatg
    52441 ttcgggcttc cagcaggagc atatgctatt tacagaaatg cacgtccaga aaacaaagca
    52501 aaaacagctt ccttgatgct agcagcagct tttgcttcat tctttactgg tgtgacagaa
    52561 ccacttgaat tttcatttat gttcgttgct tggccattat atgtcttgca tgcaattttc
    52621 actgggattt ctttagcagt gagtgcattc ttccattgga cagcaggatt tgcctttagt
    52681 gccggatttg tcgattactt cctatctttg aaaaatccga ttgcgaatca gccattgatg
    52741 ttgatcgtcc aaggattagt aacagcagtg atttactact ttggctttaa ctttgctatc
    52801 aaaaaattcc atctgatgac tcctggccgc gaggaagcag atctttcaga tgacgatact
    52861 gcaacgacta attcagacaa taaatatgcg gcacaagcaa gcaaaatcta cgctgcatta
    52921 ggcggagccg acaatgtaac ttcgatcgat aactgtacta cacgccttcg tttgcaagtc
    52981 aaagatacag gcctgatcga tcaaaataaa atcaaagcca ctggcgtacc tggtatgaag
    53041 atcatcgacg gaaaaaatgc gcaagtcatc gtaggtacag aagtacaatt cgttgcagat
    53101 gagatggcaa aactacatgg tggtgctgca gcacgacctg cacaaacaaa caccgtggtt
    53161 aaaacagaaa catttgccgg tgaaacgatc gaacaagata tttacgctat cgcaaatggg
    53221 aaactgattc caatcactga agtttcagat gatgtattcg cggaaaaaat gatgggtgac
    53281 ggctatgctg tattaccaga aaatggagaa atcttctctc caatcgctgg tacgatcaca
    53341 aacattttcc caacgaaaca tgctgtcgga atccaaacag atgctggtat cgaagtcttg
    53401 cttcatatgg gcatcaacac tgttgactta aaaggcgaac cattcacttt atatgtagaa
    53461 gaaggtcaaa aagtcgcaag aggtcaattg attgctttag ttgatcttgc cgctattcaa
    53521 tctgcaggca aaaacacgga tatggtcgtt gtattcacaa atggcgacaa agtccaaagc
    53581 ttagaaatcg aacccgcacg tgatgtaaaa gcaaatgata aaatcggctc tgtttccaac
    53641 aaagcctgag ccatcgtctt ccccagactt ttttatcgat aaacaaacag gagctgagac
    53701 aaaaatcttg tctcagctcc tttttccgaa taaaaacggc gttctcctgc caactccgcg
    53761 gtaataagtc cagctaaacg aaacatatga agagctattt tttatttgac tcttcttatt
    53821 acttgatgca gaacagcttt ttcacaaaat ttttcttcat actcaaaact aatcggcttt
    53881 ttcacaacct ctttatacat tattcctgat ccaatatttc ttttaatgct gcttcaagag
    53941 aagttaatgg ttttcccaat actttttcta aatcatcaga ggaaatagct aactgatcct
    54001 ttttaatatc atgttggatc gctaaaaaca tgtcagctgc ttcttgaggg aatccggctt
    54061 cttttagatt ttcgacaaac gctgcatcac ttgcttcaat aatttctaac tctttacctg
    54121 tcgcacgctc taatgccttg gctaattctt catagtcagt tagattgccg gataattcta
    54181 agatttctgg agagtcactt tctactagta cttttgctgc agcttctgca tattcacgtt
    54241 tcaatgccca acctgttttt ccatttcctg cagcataaac gaattgacca gtttttaaag
    54301 cctgcccaat gataggtaat tcattctcta cataccaatt attccgtaaa aatgtatggg
    54361 aaatacctga atccttaatc attttttcag tgaattggtg atctggcgct aatacactga
    54421 tcgagtgatc ggcatctgca aaacttgtat aagcgatata agatacccca gctgcttgtg
    54481 ctgccttcac tacattctta tgttcggctt gacgatttcc tggaacgcca gagacaaaaa
    54541 gcaatcgatc tattcctttc aacgcttctt tcattgagtc taaatcatca taatcaccaa
    54601 tacgtatatt aaatccagct tctttcagtg cttctccttt ttcttcacta cgtactagta
    54661 catagatttc attttgtgga accatctttt ttaagaattc cagcgcataa ccaccaaaac
    54721 ctccagttgc tcctgttact aaataatgca tatttgccac tccctattct ttttatttgt
    54781 taagatttac agctgtacat ttataaatta cacctttatc ataaagagtg tggtaagata
    54841 agtcaagcaa aaaaaatgaa aggatgacaa ctgatgaagt attctctgca atttagtgat
    54901 gcaatccatg tacttgccta tattgaaatg tttcaagata cccgatcact ttctagtgag
    54961 atgattgcca gtagtatcga aacaaatgca gcaaatgtac gtaaaatcat gagtcagctc
    55021 aaaaaatccg gtttgatctt tacaaaaacc ggaaaaccgc agccttccct tgccaaaagt
    55081 ccagatcaga tttctttact agatgtctat cgcagcatcg aaggtaatac acagcttatc
    55141 caagtcgatc caaaaacaaa tccaaattgc gtcgttggtg cgaatatcca agaagcttta
    55201 gaagaaagct acgctcggct gcagaaaaag gcagaagaag aaatggctaa gatcacactt
    55261 gatagtctga tacataaaat agccgttttg gaaaaagaaa aaagaccaga aaacacaggc
    55321 ctcgtagaga gatttttata attctctcta ctgtgcctgc atttttctga tcggaatcgt
    55381 ctacataaat tcttttattc tccgggggac aaaagccttg tcatgcccct ttttcctctc
    55441 taatgctcag gtcaaagcgc tgaagtcatg acgtcttgat aaacggtgtt caagtagctg
    55501 accctcggca ttagttctga aaaatgcaaa atatggaaag ctatttccca tttttcctct
    55561 ctaatgctca ggtcaaagcg ctactttcac gacctctata ttatcatggt ttattgacta
    55621 ccggtatcca gatttctccc atatatttcc cgttggtaac tcccatttcg actacagcat
    55681 ttggaccccc aacataggcg taatcttcta cttctcttaa aacatgccca aaggccgtat
    55741 tcgtcaaatc atttcttaat tcttcaggcg attctgctaa agcgcttaca acaagatacg
    55801 ttccttttgg gaatgggatg attcgcgtag tgtttggaac atcctgactc gtttgaacac
    55861 cgacatagta catcattttc ccgttatagg gttcgttgac tgtaaagaaa taatgatttt
    55921 cagcagcttg tttcaatgca tctattgttc cattaccgat aactttttgc tcaaatgctt
    55981 gtttctctgc agccaaaccg acaaaatccg tattgctact cttcaattcc acaccaatac
    56041 ccgtcaccgt aaaatctggt ttttcttcga tcctatactt tgccatttct cccacccttt
    56101 ctattatatt aagttcatct tacataaaat taaagggaaa tgacacgatt acgctctttt
    56161 ttctagggat atttttattt tttaactatt tttttgcttt cactgcaatc caaatctcac
    56221 tgtaaacatt actgaaatcc tctccccatt ttgtaaaaga gatctcggga gcttcgacta
    56281 acccataatc tgaagaagga agccattctg catagatttt cccccaagta tcttgtaaag
    56341 tttggggaaa tggcccttta ttaggaaaaa tggcccaagt gtgttcttct atagcaagca
    56401 tttctaaatc agcgtacgga ttttcttttg tcgtagcaaa gccaatcatg tgcatcaaac
    56461 tgcccttttc ttccaagcgg tctccatcaa atccataaga aacgttgatt acttgatttg
    56521 gatataaatc tcctaactta tgcatctctt ccctttgagt ctccgtgatc gtctgcgcta
    56581 acttttgtat ttcttgattg actccttcaa atttgacagg aacttttttc gccacacctg
    56641 ccacataaaa ctgttctttc ttttcgatac gaaattccat cgattttcct cctctgacat
    56701 caataaaaaa tgtcaagcgt ggaaaagatt tttgtatgcc gtatctactg atttctgaag
    56761 gcagatatcc tgaccaatca cgaaaagctc ttgaaaaacc ttcgactgtt tcatatccat
    56821 atctaaacgc cgtttctgtc actcttgatc catctagaag atcgcgatta gccaaagcca
    56881 atctacggtt tttgatatag tccattagcg acatccctgc aataaacgta aagatgcgtt
    56941 ttaattgata ttcagagatt cctgtatttt cggctattat tttataggaa agtccttctg
    57001 tcaaatgttc ttcgatgtaa tcgatcaatt ggttcagatc tttaagcata tttatcttcc
    57061 tccttgattg cctttttatc atattatcaa agaaagtcaa ttacttgatg aatcaggaac
    57121 caatttagtc agatgtagaa aatgttgtat ggatctaaag aaaattctca agcagcgttt
    57181 cgtaacggat aattggtatt atctgcctat ctatgtttta cctttagaaa aataaatttt
    57241 ggggaagaca gattctaagt aaacaaagcg gtaaaaatat cttttattcc cttagttaat
    57301 ccgcttacta cctactgtac aaagcaaatt gttgttcaaa ctgctgatca gaacgctgct
    57361 tggatttttc tttgcgatac atcatacctc ttttatcaat tggaacaatt atttatttct
    57421 aacaaaatca cttcattttt gtcagataaa taaaaaacaa aaaaagttcc atctttaaaa
    57481 cacgctattc ttttactttt tctcacattc tctaagaaaa gaaaaggaga atgaattttt
    57541 gacaattcga gcaaaagtga ttatcaaata tgtaaaatca gctacttttt tcttaaacgt
    57601 ttctttatac taaatataaa taagtgtttc atgaaaggag aactagatga ataattggct
    57661 tgagtatttt cctgaaaacg ttttggaacg cggttacagc tatcatctgc acggttttgt
    57721 ccgtcatttg aactatacaa gcaagtactt gtccgcaaca gtttctggca cggaagatta
    57781 taaggtagtg atcacttggg atgaaaagac caacatgact tgtgattgtc tatatgccat
    57841 agaaggaaaa aaatgcaaac acatggctgc tgtcttattc gcttacgaag aacgtccaat
    57901 aaaaaaatcc aactactcac tttccgaact ctcgtccctt gtttcctccg ctagttcctc
    57961 tcttgtacgg gagcttttaa cagagattct tatagaacat ccgcacttta tcgaacgctt
    58021 caaagttaag atgccatttc atgcaataaa ttattcagat aaattaacaa ctatcatcca
    58081 taaatacgat catattatca agaaaaacaa aaacaggaaa acagcgaagt tcatcatgga
    58141 gatgcgaaag ttcatccaag aagcagttga atcgcttatt cagcaaaatg cctatcttcc
    58201 cgcattcgaa ttgatcaatg aagtgattgc tactctcgaa acattctatt gggagcctga
    58261 ggatgaacga acccttcttc tgatagagga ctgttattat ttatggaaag aattattagc
    58321 agaagcacct catgcagaaa aaaggcaaat gttctcttgg ttcgtctgcc aagtcgatca
    58381 tactgacgcc tcttattcaa aacgctactc tataaaaatc ctgaaagaag actttcggga
    58441 aaaagaattt tctaaccaaa agaaaaaaat agataaaaaa ctaaagaaac ggtaaagaaa
    58501 gacgatttca atgaaaaata gcaagtaaac gataacaacc atcctaaatc cacaagaaat
    58561 acagcagacc ttgctttaaa aacacgaatc atgcaaagga tgaagtaaag aagacttaac
    58621 gcgtacccaa tattagtcat ttgactatta ggtacgcgtt ttgttttata agtcatgaca
    58681 aatttccttc gttgctggat agattttccg gcattgttga tagattttat gatacttttc
    58741 tacattttgt aagagaggtt ccgctgcatc tgtatattgg ataaaattct gacaacaact
    58801 ttcaggtgtg tcgaaccacc ctaaccctag tgcagcaatc attgttgcgc ctaatgcagg
    58861 tccttcctcc atacttaaac aatagaccgt tgcatcgaaa atatcagctt ggatttgtag
    58921 ccatgcaaga tttttcgcgc ctcctcccac cgaaacaata cgtgtaaatc tgcgacccgc
    58981 tagttcttta cgtttcaatc ccaccattga caaatgctca ttaaaaattt agaatattca
    59041 caagtttaac gacaattatg aaaatttatg tcgtgctaaa ggagttttgt tattggatga
    59101 aaagttagat ataaaaactg cgatcaaaga cacgttacct accgttttcg gttatatcgg
    59161 tattggactt gcatttggta tcgttgggaa agctgccgga tttcatccac tagtcgtcac
    59221 gttgatgtcc ctgctggtct atgctggttc tgcccaattt atcacagtca gcatgcttgc
    59281 tagtcacagc ccattgcttt ccatcgtttt ctcgaccttt ctagtcaatt cccgaatgat
    59341 tctgatgagt atgacgattg ctccttattt caagaaaaat cggctgcttc aaaatctgct
    59401 gattggcaca ttgctgacag atgaaagttt cgcactggga atgaataaat tgaactatac
    59461 tggacaaaag ttaaactttc gttggatgaa tacagctaat tggatttctt atttgacgtg
    59521 ggtgggttct tctcttgttg gcgcactttt agggaatttt attactgatc cgaaaaagtt
    59581 tggtttggat ttcgccatcg tcgctatgtt tatcgggtta ctttatcttc aagtcatttc
    59641 tgatagaaac acctctaaac gattacagtt gatcctgatt ggtctcactt tgattctggt
    59701 atatgtcgga ctgattttta tcccaagcaa cttagtgatt gtagttgtaa cactgattgg
    59761 gtgcggattg ggggtgtgga tcaaacatgc cttcttttga gtttacattg ctgactattt
    59821 taggttgtac tttagcaact tggctttcta gggtcctacc atttgtctta ctgaaaaaat
    59881 tcgatttacc gcaaccgttg cttgagtatt taagttttgt cccgatcgtc attatgtctg
    59941 ctttatggtt cagcagtcta tttacccaaa atatcggaca tctgccgcag atcaatctcg
    60001 aaaatgcttt ggcttcagtt cctacacttt tagctgctat tctttcaaaa agcttattgg
    60061 tgatcgtctt ggcaggaatt ctttcccttt cgttgattcg cttattataa ataaaaaaac
    60121 aagagcttat ggcaaattac tgtcatgcgc tcttgttttt atcaaagtca aataagaaaa
    60181 aaatcggcgt attcttgctt atccgttgaa cggaccacta acaacgactt ttcctaacat
    60241 atgtcctgtt tctacgtctt tggttgcttg tttcaagttc ttcgcattca tcccatctga
    60301 atagaccttg ctcaatgtac tgcggataat cccttcattt gctaaagcag caatgtgcgc
    60361 taaagcttct ccttgcgttt cgatttcata gttatagtct gttttagcga acatatactc
    60421 ccagtcgaag ctgatagatt ggtttttcca aacacctata tctaacggct ctttgatccc
    60481 tacgatcgtt cccacatgtc caaatggtcg gacaaggtgc ttgatttgat caaagtagcg
    60541 ggtaatatcg aataatacag cgacatactc tacttgatcg attcctaatt cacgaaaact
    60601 tttacttaag tcgtgatgat aatcgattgg atagtctaca cctgtttttt tcagccattc
    60661 aaaattcttc ggacttgcag tcgcgtagac ttctaggcca gcccaatgcg ctagttgatt
    60721 caaaatcgaa ccgacgcccc cggatccgtt gatcactaag atttttttcc cgcgattggc
    60781 attttcttct ggagttagac caaatttttc aaataacaat tcataagcgg tcaatgaggt
    60841 caacggcaag gctgcagctt cttcatctga aagatttttt ggtgcaagag cgacaatacg
    60901 ttcgtcaact agttggtatt cttgattgct tcctgctctt gttgttgtac ctgcgtaaaa
    60961 aacgcgatcg ccgacagaaa atttttgcac ttcgtcacct acagcaacaa ctttcccgac
    61021 tccatcaaaa cctaagatac gtaaagcatt tcttatcccg ttatcttggc gaagtttcgt
    61081 atcgacagga ttaacagata ctgcgttgac ttttaccaga agatcccgtg aactgggtac
    61141 aggtatctgt tctttttcat caataaagct ttttggatca tcgatcggca acccttcata
    61201 aaaaccgact accttcatta attccttttc tgacatacga attcctcctt agcttcatca
    61261 agttggagca tccatacagt gttcatttca atatatatag tatgctattt gttccacctt
    61321 ttttattcat cataattatt ctcttactta aagtaaacta tttgcacttt tttggttaaa
    61381 aaagaggttg cgacaagact tttgtcacaa cctcttataa acggtgttct cctaccaact
    61441 cttcggtcac aagtcaaaat attcatcaaa catgagaagc tgttttttga aattgctcct
    61501 tgtgactcag agttaaacgg caggctcaca acctctgata taaacggtgt tcaatcaact
    61561 gaccctcggt attagctcaa aaaacgacaa aatatgaaga gctatttttg tttggctgtt
    61621 cttattactt gatacaggac agctttttca cgacctctaa ttcacggtgt tcaagcaaga
    61681 aatcattgtt cactgaaaag cgttggatgt tctttttcaa aggtctctaa gatctctcgt
    61741 acttcatcag tagatttcgt attcatcaac tgaactcgca agtcatttgc tcctggaaat
    61801 cctttgatat agattttaaa gaaacgatgt agtcctgtga tggaacgcgg gatgatttct
    61861 gaataatgat cttgcagatc tagttgcaga cgaagtagtc cgatcagttc tttttctgta
    61921 tgttccctcg ggtctttttc aaatgcataa ggattcttga aaatccctcg gccaatcatg
    61981 atgccatcaa cgccatattt ctcggccaat tcttctcctg tctggcgatc ggggatatct
    62041 ccattgatcg tgattagtgt ttgtggagcg atttggtcac gtaaagcaat gattttagga
    62101 atgacttccc aatgtgcatc tactttactc atctctttac gcgtacgcaa atggacagaa
    62161 agattggcta tatcttgatg aagtaggtga ctgatccatt cttccatctc agacatttcc
    62221 gtatagccga tacgcgtctt gacgctgaca ggcaaaccgc cagctttagc tgcttcaatc
    62281 aattgtgccg ctacttctgg acgaaggatc aagccactgc cttttccacg ttctgctaca
    62341 ttagggactg ggcagcccat gttgatatcg attccttgga agcccatttc tgccatagca
    62401 atgctcattt ctctaaaaaa ttctggcttg tctccccaaa tatgggcaac catcggctgt
    62461 tcatcttctg taaatgttaa tcttcctcgt acactgtctt ttccatctgg atgacagaaa
    62521 ctgtctgagt ttgtaaactc agtaaagaag acatccggtg ctgctgcatg ctttacgaca
    62581 tggcgaaata caacatctgt tacatcttcc attggtgcta aaacaaaaaa aggctttggc
    62641 agctctttcc aaaaattagt agtcatttat ttgttactct ccattctatc aagcagtgaa
    62701 gtcgcaatct ttctcaaaaa cattgtcatt atactaggaa acaaaaaaag aagcaaagtg
    62761 aacagcttaa aagatgtttc tgattgagca aaaaaaagac tgtgacaaaa atcttatcac
    62821 agtccatttt ccggaataaa tggtgttcaa aagcttgcat ttgcggtaat aagtccaacc
    62881 aaacgaaaaa taggaggagc tatttttgtt tggctgttct tattacttga tgcagaacag
    62941 ctttttcaca acctctccac taaacggtgt tcaatcagct gaccctcggt attcgctcaa
    63001 aaaacaacaa aatatgagga actattttcc cgtttgtttc tctaatactc aggtcaaaac
    63061 gctgatttca caacctcttt tctaacttct caatcctctt cctttattga tcaaatacca
    63121 agtaacggcg tacaaaacaa cgatgaataa cacaagtacg atcatcgatg ttgctactgg
    63181 cacatcgatc gttccaagga atccgtaacg gaaaccggag atcatataaa cgatcggatt
    63241 gacttttgaa acagcttgcc aaaagggcgg taacatagag atggcataga aaacgccacc
    63301 tagataagtt aacggctgca agacaaatgt aggcacgatc gatacatcat cataagactg
    63361 agcaaaaatc ccgttgagca gtcctgctaa tgaaaacaag attgccgtca tcaataatgt
    63421 aatgatgacc atgaaccatg aatacacatg aagtggtaca aagaacaagg aaatgatcgt
    63481 tacgagagag ccgacgagaa tactgcgacc cacaccacca atcacaaagc cccaaataat
    63541 cacatgcgta ggaacaggtg ctaccaagat ttcttcgata tttttctgaa atttttgaga
    63601 gaagaaggac gaagaaacat tggcatacga actagtgata gcagacatca taatcaaacc
    63661 aggaacgatg aattccatat atgaaaaacc acccatgtca ccgatccgcc caccgatcat
    63721 ttttccgaaa ataacaaaat acaaggatgt cgtgatcact ggcggaacca gtgtttggac
    63781 ccatatacgc aaatagcggt tcgtttcctt gatagctaaa ctttttaatg cagtaaaata
    63841 aagattaaac atttttctcc tccgtatgat tattttcttc tgtgattttc aagaacagtt
    63901 cttctaaacg attcgactta ttcctcatag aaagaacttt gattccttgt cttgtaagct
    63961 gatcgaagat tccgttcacc ccttgatttc gttcaacttc gacagcgatt gtctgctcat
    64021 cttcaaatgt acaagcatat ccttcaatca caggtttggt atcatatggc gctaaatcaa
    64081 agataaatgt ttcaaattgc agttttgaca gaagctcttt catacttgta ttctcgatca
    64141 gttcacccga ttggatgatt ccgatgttgc gacaaagcat ttcagcttct tctagataat
    64201 gcgtcgtcaa aatgatcgtt gtaccatttt cgttcaattc tcgtagaaac tcccacatct
    64261 cgcgtctcaa ctcgatgtca actcctgctg ttggttcatc caagatcagc aaacgtggtt
    64321 catgcatcaa tgccctagca atcatcagtc ttcgcttcat tcccccggaa agcatacgtg
    64381 cacggacatt tctcttttcc caaagattgg actgcttcaa atatttttcg ctgcgtttca
    64441 tcgcttcttt gcgagagaca ccgtagtagc ctgcttgatt cacgacgatt tgctggaccg
    64501 tttcaaaagg gttaaaattg aactcttgtg gaaccaatcc aatctgttgc tttgctcgaa
    64561 ctagatcctt gtccagatca taaccgaaga cattcacttt tccagatgtt ttattgacta
    64621 aagaagtaat aatcccgatc gttgtagact tcccagctcc atttggccct aatagcgcat
    64681 agaaatctcc ttctttcacc gaaagatcga ttccccgcaa tgcttcgacc ccacttccat
    64741 agatcttttt caaattattt atttcaagcg catttgccat ttatttccta tctccttata
    64801 gacatgattc ttaattgtat catggaatac agcagatgaa aaaacaactt ggcttccaca
    64861 caaaaacaca cctgctaaca aagaggagat gtgttttcta gttcatgatc atcttcgata
    64921 gaataactat ctaaacaact ctggaacttt taaaatcata aaagtaccga cagcaactac
    64981 tacgatgaca tatagaagat ccttataact gaggttcttg tcttttttat tcatgttaag
    65041 ctttcctccg tccttttatc gaaagctttt ttcttttatt ttaaccgaaa taaataaaat
    65101 ggaaatagtt attttcaatt tctgttttcg ctgtttgttt tacggagaac tttgccatgg
    65161 aacgtgcgtc tcgtcgctga tgctaagttt tttcgataaa agctgctaga aggaagacag
    65221 cgtggtttat tttgtatcgt cttttgctaa atcttccaag atcatctgga cagcagactc
    65281 gttcccattg aattctgcca cttttttggc atcaatgtac ttcttttgat aatcgtcttt
    65341 ttggacccac gcatcatgct tcgtttgata aagttaatca aacggatacc actgacgctg
    65401 ttcatcgaac aacaaacttt ctttttgggc ctgttcaact atggatttag gataattcaa
    65461 aactttcagc aaggcaatcg catttcgggt ccttgaaggt ccttctttta atttgaaatc
    65521 aaaactgatt ccttttcctt ctgttacttg ttcttcaaag tggacattct cacaataatt
    65581 ctttaaaatc tctgtcaatt cgatatcatg tgtggcgaca aatgctaaag atggatactc
    65641 cgcaagccac cgaacgactg aagcggaaga agcaatccgt tcgatcgtat ttgttccttt
    65701 taaaatctca tctacgaagc agtagcagcg ttctttggaa gccacttggt ccagcaaacg
    65761 tttgatcgat ttgatttctg aaacaaaata actgtcgcct tcaaataaat catcttcgat
    65821 tgccatggaa gtcaacacat gtccaggttg cagggtaaac tgttctgcaa tcgctgtttg
    65881 gatcgtctgt gctagaatac agctgatggc tatacttttg acatacgtag attttcctga
    65941 tgcattggaa cccgtgacta atgtattctt ctgccagttg actggattaa ctaccgcttc
    66001 ttttaacaat ggatgataga tatctttcgc tgtcacaccg ccctcagtaa actctggtcg
    66061 gcaagtgatc ggcatatagg tgcgataatt caaaacggct gctgcaactt ccaattctcc
    66121 cagcaattcc cacagcttga tggcttcagt tgaatgcttt tctaatttag tgagaacgaa
    66181 atgataggag ataaatggca gcatgaacat gatgttgaca tattcaaaaa tcaattctgc
    66241 ttcactcccg ctcttcattc gaaatgaaaa acctaattta gggatgtctc ttaatggttt
    66301 agtttgctgt ttcagctctt tttgaagtgg tgtcgtcatt tttgctaact gattggcggc
    66361 tgaaatcgtt gaaaccaaat aactcatgct attcagctct gtttctagtg tttgtttctt
    66421 cactagataa tagaccgtat tgaatactaa gccaccgatt gctagataga ttcctagtac
    66481 ttgccctaaa agaagcaaac ctacacccac cagcggaagc aagccaagaa aaataaataa
    66541 acctacgcgt ccaatctttt tactttttcc atccgctaga tattgttttg caaaattatg
    66601 atcttttttt cctaaacgag cgaattgata ttggattctt tctctcagtt gaggattttc
    66661 ctgataaaaa gcaattagtt tttctaactg ataatcttct ccaaaatcaa agctacgtaa
    66721 acgctgatac aacgcttctg aacctacact agaataggta ctgttgatcc cttcaaatac
    66781 ttcgaacata tccaagtcat accaagtaag atcgtcgatt tcactatccc atgaacggaa
    66841 ttttttctcc gtgagccacg catccttcaa actttcttct ttatcaaatc tcggttgata
    66901 aggaattttt ccccaattac tacgaactgt ttcctttact ttcaaacgtg aaaaaatgtc
    66961 taaaacaact aataaaataa caactgcaat aataattaaa acgataaatt ctctttggcc
    67021 catatccctc tccgttcctt gatttgttag ttttagtata gcactgttgc gctatttgaa
    67081 aagggggtac aaaaataact gtcttcgtta cctaattcgt atcaatcaca ccaaaataaa
    67141 aacaccttcc aaaaaagaag atgtctttaa aacgagatac aattttttgt cattcacttc
    67201 aggtaaatta gagccaatta aaccctccgc aacttgacgg agatggagaa gatgcccccg
    67261 tctaatctag cggtcatctc tcctcccatt ccttccacca atttttttga caggtaaagc
    67321 ccgattccgc tagattccgt atttgtgcgg ctggtatttt cggtataaaa acgctggaag
    67381 actttttcaa cagaggtttg cgctcctggt ttgataggat tggccactgt taatcgaatg
    67441 aatgaatcct cttttgaaaa tgtcaccttt gcttgtccat ctgcataacg caataaattc
    67501 tgtatgatat tttgcagcac tcgcagcaac atgagcggat ctgctaatac agttgtattt
    67561 atcgagtgtt cagcgaacgc tacctcgata tttttctcag aaaattgttc ataaaaagag
    67621 aaaaatactt cttccacctc tttagaaaaa gaaagcgtat ggagatccat ttctttttgc
    67681 ttcgtctcta ttcgagcaag atcataaaac tgatccgtca tactgagtaa tcttgcaaca
    67741 gattgtttga tgatcgttaa atacttctcc cgtttctccg tatcctctac ctgttccagt
    67801 aattgtacat aaccgttaat agcagtcaaa ggagttctga gatcatgaga aaggccgacg
    67861 atcgatagtc tgatgttttc ttccatttct tgaaatgctt gtcctttttg ttcattttca
    67921 tccacccatg tattcagtgc atctactagg tccattagat tcttttctcg aaattcgact
    67981 gaaagacgac caccatattc ttgtcgttca gaaagagtgg cgattttctt agttaatctc
    68041 ttgatttccc tttgcaggaa gaaaataaaa cttcccataa agataacaac gagaagcaac
    68101 agccacttaa tcaaatctaa accccactcc ccacaccgta tgaatataca cagcatgtgc
    68161 ctttgctagt ttccctcgaa gattggaaat atgcatattc actgttttct cactatcaaa
    68221 atatggttct tgccaaactg tttcataaag atttgctcgc gaaaatactt ttttcggatg
    68281 ttctaaaaaa agcttcagca gatcgaattc tcttcctgtc aaatggacag ccacaccatt
    68341 cacatttaca tccctcgact ctttgtccag taagatttcc ttgtaataga tgcgttgcga
    68401 cagatctttt acttggacag aagtctgatg acgaagttgg atgccgattc gtaacaacag
    68461 ttcttcgata tcaaatggtt tcgtcacata gtcatccgct cctgctgcca acaaggaaac
    68521 tttatctctt tgatcgcttt ttgctgaaag aacgatgatc ggaacattcg atgtctgtcg
    68581 gatttctgct aacaatgctt cacctgaaag ccctggaagc atgagatcta gaaggatcaa
    68641 atcgacagga tatgtgctta acaataattt tccttccgta cctgaataag catcaagaag
    68701 tttgtatctc tgttcaagaa cctcctccag taaagaatga acagcttcat cgtcttcgat
    68761 aatcaaaata gtttccagca ggaacaccta cttaatgtca gatttttgga aaataaacat
    68821 tcctagagct gatgatacca cgattgttac tagactgacc agaatcattt tcggtgcttg
    68881 cgaataagtt gcttgttgcg caaagtccgt atacatgagc cacgtttccg taaaccactc
    68941 cgttagaaat tccaagcctt tgatttttgc gaaaacaccg ggaacgaagg ccgttcccat
    69001 gatataaaga aaagaaagaa gcaaactgac actggtactt ctagtgatga atgtcaaagc
    69061 cactggaaca gataatgtcg ctaatatcag cagaagcaat acaggaaata cttttagaac
    69121 ataatttcca tagtttttga ttccctcaac ttcaccaaat ccgaaagcaa gtgtgtaagc
    69181 aatcatggct agccccgtaa tcaaaaccac gccaagtgtt gtgatcaaaa gcgtaacgag
    69241 aagtttcgac acataataag tcattcgatt ccgtcctaaa gataacgtat tgcgaatcgt
    69301 accgttttga aattcttccc caatataaaa gcacgcaaat gctgcgaaag ccagaagaat
    69361 ccatgcagcc attttttcca ccattaaaat ccctgttgca ccgttgatga ctgtatccgt
    69421 tgcttggttt aattcattgc cgtattttgt atgcatatac cattcgttcc aagaataaaa
    69481 aagcggaagt acgaccatca gtcctagtga gacccacatt ttacggctct tccatacttt
    69541 tattttttca gcagttaata gattaaacat gtggattgcc tccagttaat tgtaagaaat
    69601 aatcttccag tttttgccca actaggtgga taccatccac aggaactttt gcctctacca
    69661 atgcttgatt gatggctgct acttggtcaa ttccttcaaa aagctggata acacggctag
    69721 attggacaaa ataatcccta taaccaagag agtcgagaac cgttactgct tgttccgtcg
    69781 ccgaagtgtc aatttttata aattgacgag attcttgtgc caattcttct ttcgataatt
    69841 ctttgatcaa gtgtccttca tgcaaaatac cgtagtgagt ggcgatctgt gacaattcat
    69901 ctagcaagtg gctagaaagc aagacggtga tgccccgttc ttttaccaaa cgttggatga
    69961 tttctcgcat ttctgccatc cctgctggat ccatcccatt cgtcggttca tccaaaatca
    70021 aaaattctgg attcgtgatc agtgcatttg caatagctaa acgctggcgc atacctaaag
    70081 agaaattctt tgctttcttt ttccctgtat tttccaatcg catcattttc agcaggtctt
    70141 cgatttctct atccgacaca ccgccgttcg ctgcttggat acgtaggtta tctcttgcag
    70201 ttaattctgg ataaagagca ggggtttcaa tcgattgacc catacgtcgt cttgctgcct
    70261 gcaaatccgt cgttccaaat aattcgatcg aaccttcatc aatcgagatc aaacccataa
    70321 tagttctcat aaaggtagac ttacccgctc cattctcccc aatcaaacca tagatcattc
    70381 cacgtttgat ttcgatcgat actttatcta atgctttggc agcaccgtat tttttggtta
    70441 taccagttgc tttcaagacc gtttcatttc caaaatccat acttatcctt ctttcttatt
    70501 gataagtgaa gtatagttga cgggtataaa gaaagcagcc agaaatgtat aaagaaagtg
    70561 taaattttcc aattgaattt caagaaaaat cagactgtct cgcagacgtg gttgagttgt
    70621 ctgccaatca aagagcgcac caaacagtgc taatccaagt ggactggcca ccatggtaat
    70681 cgttgttatc gttgacatca gacgtccttg tatatgcgtt ggaacttctc gctgaagcca
    70741 gaccatataa aaagcaaaag aaaacattaa tccttaaaaa cggacttatg aaaaataata
    70801 catgccgttt gggatttaca attagctacc aaataagagg ttatgacaaa agacttttca
    70861 caatcccttc ttccgaataa acggtgttct cctgccaact cctcggtcac aagtcacaat
    70921 atttatcaaa catgagaagc aatttttgta aattccttct tatttcttga agctgaccac
    70981 ttctgtcaca acctcagaag cggataaata atattgtttg atgatacttt atctttttcc
    71041 cgtttgttta tagatcgaat gatattatag accaagaatc ctacctatta acatagctaa
    71101 aaacacaaaa aaggcacttt ccaaattgtg gaaagtgcca gaaaagttag gattaaggta
    71161 actgcgctca gcattgcttc gcccagtacg attctcgtaa ctcgtgaact cgaacgataa
    71221 tcgcaattaa cgttttgaga actgtgaagc tttacgggct ttcttaagac ctggtttttt
    71281 ctttatatca ttgaatagcc ttgaaaaacc ttgatataaa aaggttttag taaaaatgat
    71341 tttatcaaat ttgaataact ttgaaatatt tgtgttctaa aatgtgttct aatttaaagg
    71401 tagtatattt
//
LOCUS       DAZABD010000006        69032 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000006 DAZABD010000000
VERSION     DAZABD010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 69032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 69032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69032
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.006"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(257..2443)
                     /locus_tag="ACHALG_000428"
     CDS             complement(257..2443)
                     /locus_tag="ACHALG_000428"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFN2585868.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            2689..3372
                     /locus_tag="ACHALG_000429"
     CDS             2689..3372
                     /locus_tag="ACHALG_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HFN2585869.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            complement(3453..4172)
                     /locus_tag="ACHALG_000430"
     CDS             complement(3453..4172)
                     /locus_tag="ACHALG_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFN2585870.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(4484..4690)
                     /locus_tag="ACHALG_000431"
     CDS             complement(4484..4690)
                     /locus_tag="ACHALG_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2585871.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            4935..5537
                     /locus_tag="ACHALG_000432"
     CDS             4935..5537
                     /locus_tag="ACHALG_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFN2585872.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFALEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            5678..6958
                     /locus_tag="ACHALG_000433"
     CDS             5678..6958
                     /locus_tag="ACHALG_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFN2585873.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTLLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIALSAFLSALLAFCFKKTLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLVVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            6971..7699
                     /locus_tag="ACHALG_000434"
     CDS             6971..7699
                     /locus_tag="ACHALG_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFN2585874.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(7768..9291)
                     /locus_tag="ACHALG_000435"
     CDS             complement(7768..9291)
                     /locus_tag="ACHALG_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFN2585875.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVASI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFITQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYIEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKDGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEALLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            9543..10724
                     /locus_tag="ACHALG_000436"
     CDS             9543..10724
                     /locus_tag="ACHALG_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFN2585876.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSVW
                     LVPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYQNSSFSEEDYF
                     K"
     gene            complement(10784..12412)
                     /gene="treC"
                     /locus_tag="ACHALG_000437"
     CDS             complement(10784..12412)
                     /gene="treC"
                     /locus_tag="ACHALG_000437"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFN2585877.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(12933..15644)
                     /locus_tag="ACHALG_000438"
     CDS             complement(12933..15644)
                     /locus_tag="ACHALG_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFN2585878.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNAKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKAAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(15654..16355)
                     /locus_tag="ACHALG_000439"
     CDS             complement(15654..16355)
                     /locus_tag="ACHALG_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN2585879.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            16540..17139
                     /locus_tag="ACHALG_000440"
     CDS             16540..17139
                     /locus_tag="ACHALG_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFN2585880.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKGIVNNGTLSPHTAQSILSNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTNN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHVVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            17220..18233
                     /locus_tag="ACHALG_000441"
     CDS             17220..18233
                     /locus_tag="ACHALG_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFN2585881.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQISHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEESGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            18342..19259
                     /gene="rbsK"
                     /locus_tag="ACHALG_000442"
     CDS             18342..19259
                     /gene="rbsK"
                     /locus_tag="ACHALG_000442"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFN2585882.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            19287..19682
                     /gene="rbsD"
                     /locus_tag="ACHALG_000443"
     CDS             19287..19682
                     /gene="rbsD"
                     /locus_tag="ACHALG_000443"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFN2585883.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            19682..21055
                     /locus_tag="ACHALG_000444"
     CDS             19682..21055
                     /locus_tag="ACHALG_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFN2585884.1"
                     /translation="METTMNPEQMVLYEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRDNMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            21412..23562
                     /locus_tag="ACHALG_000445"
     CDS             21412..23562
                     /locus_tag="ACHALG_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFN2585885.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQTADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFEPVPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNN
                     NNGSNNSNNNNNNSNNGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPN
                     TANQSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFEHLGKPYVWGAKGPN
                     TFDCSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVG
                     IYIGNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPATSGSLTGSSILDGLDGS
                     FHFSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLF
                     GIKGSYNGSSVTFNTHEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDF
                     YKPTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEA
                     NAEILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQ
                     IPETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(23614..24654)
                     /locus_tag="ACHALG_000446"
     CDS             complement(23614..24654)
                     /locus_tag="ACHALG_000446"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFN2585886.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSLDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(24930..26732)
                     /locus_tag="ACHALG_000447"
     CDS             complement(24930..26732)
                     /locus_tag="ACHALG_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN2585887.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAEKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQTTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(26746..27498)
                     /locus_tag="ACHALG_000448"
     CDS             complement(26746..27498)
                     /locus_tag="ACHALG_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN2585888.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(27507..27869)
                     /locus_tag="ACHALG_000449"
     CDS             complement(27507..27869)
                     /locus_tag="ACHALG_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFN2585889.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(28045..28917)
                     /locus_tag="ACHALG_000450"
     CDS             complement(28045..28917)
                     /locus_tag="ACHALG_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFN2585890.1"
                     /translation="MREKRAFYINGYAGIVGLIVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(28968..29456)
                     /locus_tag="ACHALG_000451"
     CDS             complement(28968..29456)
                     /locus_tag="ACHALG_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFN2585891.1"
                     /translation="MKAEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            complement(29594..30385)
                     /locus_tag="ACHALG_000452"
     CDS             complement(29594..30385)
                     /locus_tag="ACHALG_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HFN2585892.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADYELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            complement(30440..31048)
                     /locus_tag="ACHALG_000453"
     CDS             complement(30440..31048)
                     /locus_tag="ACHALG_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HFN2585893.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(31041..31358)
                     /locus_tag="ACHALG_000454"
     CDS             complement(31041..31358)
                     /locus_tag="ACHALG_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFN2585894.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(31515..32243)
                     /locus_tag="ACHALG_000455"
     CDS             complement(31515..32243)
                     /locus_tag="ACHALG_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFN2585895.1"
                     /translation="MPTGVLINVGSVLLGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            32403..33284
                     /locus_tag="ACHALG_000456"
     CDS             32403..33284
                     /locus_tag="ACHALG_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFN2585896.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITDFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(33524..34564)
                     /locus_tag="ACHALG_000457"
     CDS             complement(33524..34564)
                     /locus_tag="ACHALG_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFN2585897.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRVIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFLQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQVNGKIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAIIRLSK"
     gene            complement(34567..35235)
                     /locus_tag="ACHALG_000458"
     CDS             complement(34567..35235)
                     /locus_tag="ACHALG_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFN2585898.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     HIELIITDVMMPEMDGYDLVGSLRNARFDMPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSILTIGQTKLELDTYTVVQNQHSFILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            35377..37713
                     /locus_tag="ACHALG_000459"
     CDS             35377..37713
                     /locus_tag="ACHALG_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFN2585899.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTDHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSD
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(37828..38139)
                     /locus_tag="ACHALG_000460"
     CDS             complement(37828..38139)
                     /locus_tag="ACHALG_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2585900.1"
                     /translation="MKKMEESFPKAAKVENIANILKVTFENGEVKYVKSHWTEEITDD
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            38320..39795
                     /locus_tag="ACHALG_000461"
     CDS             38320..39795
                     /locus_tag="ACHALG_000461"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HFN2585901.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDVIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGMSAKTIFDGTVPFDMKEHFVRAKFQEVKDWEKYLK"
     gene            39842..40426
                     /locus_tag="ACHALG_000462"
     CDS             39842..40426
                     /locus_tag="ACHALG_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFN2585902.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLNEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFGIVNEHSKRWQGD"
     gene            40428..40847
                     /locus_tag="ACHALG_000463"
     CDS             40428..40847
                     /locus_tag="ACHALG_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HFN2585903.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGWFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(41128..41412)
                     /locus_tag="ACHALG_000464"
     CDS             complement(41128..41412)
                     /locus_tag="ACHALG_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HFN2585904.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(41764..42474)
                     /locus_tag="ACHALG_000465"
     CDS             complement(41764..42474)
                     /locus_tag="ACHALG_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HFN2585905.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(42471..43943)
                     /locus_tag="ACHALG_000466"
     CDS             complement(42471..43943)
                     /locus_tag="ACHALG_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFN2585906.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLTKAGDNA"
     gene            44081..44554
                     /locus_tag="ACHALG_000467"
     CDS             44081..44554
                     /locus_tag="ACHALG_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFN2585907.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(44707..45258)
                     /locus_tag="ACHALG_000468"
     CDS             complement(44707..45258)
                     /locus_tag="ACHALG_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFN2585908.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            45363..45893
                     /locus_tag="ACHALG_000469"
     CDS             45363..45893
                     /locus_tag="ACHALG_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HFN2585909.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(46379..46822)
                     /locus_tag="ACHALG_000470"
     CDS             complement(46379..46822)
                     /locus_tag="ACHALG_000470"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN2585910.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(46838..47998)
                     /locus_tag="ACHALG_000471"
     CDS             complement(46838..47998)
                     /locus_tag="ACHALG_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFN2585911.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTIAEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            48874..50574
                     /locus_tag="ACHALG_000472"
     CDS             48874..50574
                     /locus_tag="ACHALG_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2585912.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNIRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFNKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKHLRA
                     "
     gene            complement(50676..51509)
                     /locus_tag="ACHALG_000473"
     CDS             complement(50676..51509)
                     /locus_tag="ACHALG_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFN2585913.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLAKKTLESQVLTVSQSQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEEGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(52059..54224)
                     /locus_tag="ACHALG_000474"
     CDS             complement(52059..54224)
                     /locus_tag="ACHALG_000474"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIBC"
                     /protein_id="HFN2585914.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNRQADS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLEVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            54484..56778
                     /locus_tag="ACHALG_000475"
     CDS             54484..56778
                     /locus_tag="ACHALG_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HFN2585915.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFAGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEATITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            56771..57436
                     /gene="pgmB"
                     /locus_tag="ACHALG_000476"
     CDS             56771..57436
                     /gene="pgmB"
                     /locus_tag="ACHALG_000476"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFN2585916.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDRTFNEQLK
                     GVSREDSLSLLLAHGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            57429..58436
                     /locus_tag="ACHALG_000477"
     CDS             57429..58436
                     /locus_tag="ACHALG_000477"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFN2585917.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEELPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRKMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            58458..59477
                     /locus_tag="ACHALG_000478"
     CDS             58458..59477
                     /locus_tag="ACHALG_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN2585918.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDPVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(59758..61125)
                     /gene="nhaC"
                     /locus_tag="ACHALG_000479"
     CDS             complement(59758..61125)
                     /gene="nhaC"
                     /locus_tag="ACHALG_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFN2585919.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(61234..62655)
                     /gene="tyrP"
                     /locus_tag="ACHALG_000480"
     CDS             complement(61234..62655)
                     /gene="tyrP"
                     /locus_tag="ACHALG_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070908 - tyrosine:tyramine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-tyramine antiporter"
                     /protein_id="HFN2585920.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(62898..64775)
                     /gene="tdc"
                     /locus_tag="ACHALG_000481"
     CDS             complement(62898..64775)
                     /gene="tdc"
                     /locus_tag="ACHALG_000481"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFN2585921.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FTDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(65066..66322)
                     /gene="tyrS"
                     /locus_tag="ACHALG_000482"
     CDS             complement(65066..66322)
                     /gene="tyrS"
                     /locus_tag="ACHALG_000482"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFN2585922.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEVEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(66434..66639)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            66902..68203
                     /locus_tag="ACHALG_000483"
     CDS             66902..68203
                     /locus_tag="ACHALG_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFN2585923.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(68272..68871)
                     /locus_tag="ACHALG_000484"
     CDS             complement(68272..68871)
                     /locus_tag="ACHALG_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN2585924.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
BASE COUNT        20714 a        13940 c        12292 g        22086 t
ORIGIN      
        1 aataactgat tatttacgtt tgaataatga gaattattct atatattctc tattttgata
       61 atagtattct catttttaaa ataaataagg gaggtgtgac aaaattcttg tcacacctcc
      121 tttttccgaa taaactgtgt tccagaaata gcttcttcga aaataagctg aagtttcaca
      181 aaaatttgag aagcaatttt cgtacattcc ttcttctttc ttgaaactga ccacttctgt
      241 cacaccctct cttctatcac tttccttttt tcacacgact cccggtttct ttgataaatc
      301 tcgatacttt caattttctt gaatttttca tttgaggtac acatagatac agacgatttc
      361 ttgctctagt catcgcaaca taaaatagtc ttcgttcttc ttctatcgtc gtcactggtt
      421 ctgcttttat ttcattaaag tcgattactt tcttttctgt cgctccgtta taaggcaaac
      481 ttccttcgtt acaaccaatc aaaaagacat ttcggtaact aagaccttta cttttgtgaa
      541 tgctctggat acatagtgca tcaagttttc cttgtctgta agcaatcaag cgggttttta
      601 attctttagc tgtcgcttgg gtttcttgcg ataaggcttg gagtcttctg aaagtagcat
      661 gctgctttac ttcttctcgt atccattctt gatcactctg gtcgatttcc ttagctatac
      721 gtggcagatg aagatagctt tgaacaagcg aatagatttc ttgatgctgg tctgttttac
      781 gcaacgcttc ctcaaactct cggatttctg cagatttcga ttttaaattg gggcttatcg
      841 acaaaatata atctgcaacg gctatatttt gatatttagg agaaaagtat tgatcgcatt
      901 cacttttttt caggtagcag tctgccatca gctgccggag caattgctgg taggcctgat
      961 ttttcttttg cagattcagt gctgtttgcc aatgaacaat tttttgatag aaatgttgga
     1021 aagacttgat accaaagata ccttcatatg gactatctgc ttccagtaca aaaggaatat
     1081 tcgttttcag cagagattgg acaagctgct gcgtttgtga agctgatcgg tataatatga
     1141 ttgattcctt atatttccca tttttttgaa taaatgtctg aatttgtttg attagccact
     1201 gactttctgc tttagtatca gaaaacaaat aggcttccat aacacctgct tctttttgtg
     1261 ctggaatcat cgcctttggt atccgatcat ttttattata gctgatcaat ctatttgctc
     1321 cttggacaat agctgcccga gaacgataat tagttaataa gaaaagagtt ttggtaggat
     1381 attctttttc aaagttcttg atgtatgctg gctctgatcc tctgaatcca tagatactct
     1441 gatcatcatc tccaacaaca atcaacttga tcctgttttc ctttgtcatc cgtttcacga
     1501 tttcccattg aagtggatta gtatcttgaa attcatcaat gaatagataa tcatattttt
     1561 ggccgatata atgctggatc tcctcattct ccaaagcgct tttcagattt tggagtacat
     1621 cattgaactc tagcaattgc tggcttcttt tttgttcttg gagttccgtg agcaatccat
     1681 acagtcgttc tgatatcggc tgtcgtgcat tcttatcaat ggaatcatat ttttctttaa
     1741 ctaactgaac catttcttct actgaaaggt tcatattctg ccaatagtct aaccgagaag
     1801 caatcgtttt ttctccatat tgatcaaata tataaagatt atatttacgt atcagctgtt
     1861 gcaaaacagc attcactttt tgtgcagtat cctttgaaaa gtctccttgt attccttgca
     1921 ttgctacttc aggaaatttt cgacaaattt cattgtatat cctaaaaaag atactatgga
     1981 atgttccgat atggacagcg gtttttctct ggctgaaatt atctactcgt ttttttagtt
     2041 catttgcagc gttccttgta aaagtgatca ttagtatgcg ttcttcgtct acttgttttt
     2101 gcgtcatcag ataaacagct tttgcacaga ttgtttgcgt tttgccgctc ccagctcctg
     2161 ctatgattcg aagatgtttc ttcatatcat atgtgatagc tgctttttgt tcttgattga
     2221 agcgattata ggaacgtttc tccatcattt taaagaaaga gctgtttcct tcactttctt
     2281 ttacttgtac ttcttcctca ataagtccgg gtgcttcgcc ataagccgct tcataggtag
     2341 caatgatttc ttcttgtagc cgaaagactt gttgaagctt tttatacttg ttctcccagc
     2401 gtttcaattc tttttgtaat cggagaattt ctttttcatt caactgattg atttccttcc
     2461 catctttctt ctttgaattt gtttatttca agttggttat ttaaagttgg ttatttaaag
     2521 ttggttattt aaagttttgt tgtctatttt ttctttatac taacagacct ttctaaaaat
     2581 gaaaatggga agcgatatga ttttagtttt ctgaaaattt ttcctttttt tcaatcgttt
     2641 atgctaagat aaggaagaca aaaatattca taaaaggtga tgaaaaaaat gcagacttcc
     2701 tttcacgagg ggcgtacaat aggacttttg ctgacattta tcggaggagc catggacagt
     2761 tatacttata tccaatatgg tgcttttgcc tctgctcaaa ctggcaatat cattctatcg
     2821 atcatccaag catttgacgg agaatggcta agtgttggta aaaaagtatt gtctaccctt
     2881 ttctttttct tagggattct tctaacaaag tatctgattg attattttag aaaaaaagaa
     2941 aaacatttct ggcgcttgtt tgttctatat tatgaagcag tgattttttt ctttgttagt
     3001 ttgacacccg tccatgagca tcccgcatta gtaacgattt tgattgcttt tactgcatcg
     3061 atccagtggg tagcttttga taaaatcaac gggcgtgcct acactaattt atttacaact
     3121 ggaaatttaa aaggggtagc aacgaactta tatgattatt gtatttctaa agaaaaagca
     3181 gattttgatc gatttttcca ctatctaagt gtcgttcttg cctttatcgc aggagctatt
     3241 gtccttgttt tctgtcatca tctgtttggc gcccattcga ttttgatcgt ttccatttta
     3301 tttttgatca tggccctcac tcagacattt catttatggc aattttatca tacgaatcgg
     3361 ttcactattt gatctcaatg aatcaaaaaa actaagcaag atgctgctca cagagaaata
     3421 tctgcgagtg agcaatttgc ttagttttta tattatttag ttgtaaagat tccctttgct
     3481 ttcgggttat ttgtagtgta gcggatcgac tgggcatctg cctcttctgt cattttcttt
     3541 gcccgcatct tgatttcttg tacgatttgt tctccacatg ccggatcggc tacgaaatca
     3601 tatttgtccc catcgatcac tattttggga catatatcaa actgttcata ccactcttcg
     3661 tatcgtcgat tgagttcttt atagtaggaa tagagttcag gatcttgttc tagctgttca
     3721 tattcccggc ctcttttttt gattctcgct aacatagttt caaaggatac acgaatataa
     3781 accagtagat ctggtttctt ttgtggcgaa atttcttcta actccttcag catcgtttct
     3841 aaaagacctt catactgctt tacttctact tcagaaacac gccctaaatc tgcattcaaa
     3901 tgaaacagca tactatcttc gtagatggag cggtctagta cgttgtcatc atggctgaaa
     3961 gcatctttga ttgccaaaaa gcgtttgttc aaaaagaaaa tctgtaataa aaaagtatac
     4021 ttttctggat tagaataaaa tagcggcaat acttcattat cttccacgtt ctcataaaaa
     4081 ggttttgttt ctaaatcttg ggcaagcatt tcagtcagtg tgcttttccc tgcaccgatc
     4141 gttcctgcaa caatgatcac aattatcggc atccttcctg taataaaatc tgttcttcat
     4201 tttactactt catttataga aataaaagcc ttgactttat ctgctcctta aatgctactt
     4261 ctctaaaagt agccaaaagt aactcctatt catgaaaata aaggctgtga caaaagtctt
     4321 gtcgcagccc ttttttcaaa taaacagtat tctcctgcca acacctcggt cacaaggcaa
     4381 tattcataaa acatgaaaag ctgtttttta acattattcc ttgtaactca gagctaaacg
     4441 ggagactcac aacctcttta tgctctcaac attttatctg tttttattct tctattgtac
     4501 ttgatttgcg tacaactaac tgccagtgca gattatgagc ggtatcattg tcttctactg
     4561 cgcatttcat gacacgtatc atcacactat tttcatagat tttttctatt tttcctttta
     4621 ttggtgattc aaataaatca gatttaaatg catatgtttc gccgacttca aacaattgtt
     4681 cattttccat aagaatccct ccatcacctt ttataaggtc agtaattttt taacataaat
     4741 cactcgaaaa agcacaaact tgtcataaaa agtacttttc gtatcaaaac tgtcactttt
     4801 tttggtaagg ttcttggaaa acgaagattt ttttaggaaa tcatgacggt tttgatgtca
     4861 aaaccacttt ttatgaaaca aaagacgttt tttatttttt ttagcataaa atttgtggtg
     4921 gaggtacgta tgagatgaaa gataaagttg tgaacaaatt attagaagcc ttattcagcg
     4981 gagacaaatc ggaagaagat atggtgtatg tccaagtaaa atttgcctta gaagttttat
     5041 tgaataatct tggaaaacta gcggtcgttc tagtcttttc tctggtgaca ggctcatggg
     5101 cggaaacagg aatcaccttt ctttcttata tctgcatccg cagatatgct tatggattgc
     5161 attccgactc tgaattcgtt tgtctgctgt ggacactact ttatttatgg ggcgttccct
     5221 tggtgatgaa acatctgcaa ttgactatct ccttcccaat gatggtcttt ttgttaatca
     5281 gctgcttttt gttactgctt cgctatggat caagaggtac agcaatcaat cccattgaac
     5341 ccgagaaacg tcccccatta ttaaagaagg cgatcagcat gtttctaatt ttttctctca
     5401 ttactttatt tttctcagct tcttactttt ctacctattt gctacttggg attgttttag
     5461 aaatagctac gttgcttcct attacaaact acttgatgaa ttttggagga atctttcatg
     5521 aaaaaaacaa tcattaaaca attaactaac ttatctgact tttttgtatc tggctgctgc
     5581 tttggtatga gttctgaatt caaaatccct gaaaaattaa aaaaataata cgctaattgt
     5641 gctataattt ggtaaaaatc aatgggagct gtactttatg tttttatggt ccatagcgtt
     5701 gcaatatttg atttacacaa tttgtatctt attgattgac catcacatcc gatacaaata
     5761 tctgtttgcg ggagtcattc tgatttcttg tattttgatt ggatgggtct attcgatttt
     5821 agggattttt actgctctaa ttgctatagg aatgctgatg cttacttttt tcttcttttc
     5881 gacttcgcgt caacaattgg tttacgccct tccttttggg ttattgacgt tgcttttagg
     5941 agatcactta acaagcatcg gcgatttttt tgttttgaaa tcttcggtta ttgaaccagg
     6001 agattcattg cagtattttc acattgcctt atccgctttt ttatctgctt tattagcttt
     6061 ttgttttaaa aaaacgttag agacttggtt tcacactgtg gaccgccgta tgatcggtac
     6121 gatcgggaca ttgatattat ttacatacta tatcattatt ttttataccc gtttttctgg
     6181 tgaaacacct gatgtcttag ctttgaatac gagttttttc attctatatc tgtgtgttgg
     6241 cctgatcatc ttggtttatt attggaaaat ctctaataag aaattagctg atcagttatt
     6301 agagcaacaa gtcaggatgc aacaggatta tatcaaagat ctagaaaaaa actatcaaga
     6361 cttgagagaa ttcaaacatg attaccaaaa tctgcttttt tctcttcaca gctatatttc
     6421 agaaggcgac ttagaaggat tacggtctta ttatgatcaa acgatattac ctacacgcca
     6481 gatgatgaac ctttttccgg ccaatctaag tatgttggat aatatgaaag ttcctgagat
     6541 ccgtagttta ctgtcattaa aactcatgat ggcacaggaa aaagggttgg tcgtccagct
     6601 tcattttcct gagccaatcg aactggatac gaaacatacg gtaaaccttg tacgtatgct
     6661 tggtattatc ttggacaatg cgattgaagg agctagtgct gcaaagaaga aaaaaatcga
     6721 attgaccata ctcaagaaga atcattccgt aatgattcgt gtgatcaata caacaacaaa
     6781 tacattgccg ttaaaccagt tgaaacaaaa agggttctca acaaaaacaa atccaaaaaa
     6841 cgaaggcctt ggcttgttta tcttggatga attgacaaag accaatcctt atcttttcct
     6901 tgaaacatca attgaaaacc agcgtttttc tcaaacgatt tatatacaaa taaaataaag
     6961 gagtaatgat atgattccca tttatatttg tgaagacgat ccaaaacaat tagaagtttt
     7021 atcaacggtg atcaaaaata gaatcatgat tgaaaactta gacagttatg tccatatttc
     7081 tacctctgat ccggaagaac ttctcaatgc agccaagatc agaacttctt cttttggtat
     7141 ttacttttta gatatcgaac tagaagacag tgaaatggat ggtgtagcga tcggtaaatt
     7201 gatccgtgaa ttagatcctt taggaaaaat aatctatgtg acttcccata cggaaatgtc
     7261 gatgaaaatc ttaaaaagca acattgaacc gactgattat atcgtcaaag aggatcttta
     7321 tgatttaaaa gaaaatgtcg agcatatcct gagcaaaatt ttcgaagatt ttcaaatgtc
     7381 ccagatccaa aaagatatct tcaaaataga gtttaatgat gagatcaaat ttttaccgat
     7441 caaagacatc caatattttt ctacttcgct aggagcacca cataaactgg aagtccattt
     7501 gactcatgcc caattacaat tctatgagaa gatcaaagaa atcgagaaaa tgcatcggaa
     7561 ttttatccgt tgtcataaat cctatgttat caatacacaa aatgtgcggt cgattaataa
     7621 aaaaaccaga gaagtcacgt tggcaaacgg cgagaccatc ccagcctcta tccgtggatt
     7681 aaagaaattg atttcataaa aagaaccgaa tagtttggac cgactaaatg accagccgat
     7741 ccaaaccctt cggttctttt ttgcctatca gtgcaaaagc atttcctgtg caatttcacg
     7801 tccatccatt gtatctggat aaaatgttgg ccaattctcc atttctttca gcaatgcttc
     7861 ttgactttca tttcctgcat ataaatgaaa atgttcggag tcagccggtg caatcgtgtg
     7921 atcactgaat tgaatatatc taaaagcgcc actttcctta tcttctgctt cgaatagata
     7981 ccttacgcca cggtttcctg cctcgtaggt caagatttct ttccctacat aacgataatt
     8041 tgaacgtttc gttgttccat ccttaaacgt aaatgccatc tctttctcac tgatcttgat
     8101 tgaagcaata tctgtttgat agcccttcgt ataatattct ttatattctt gtgctgtctt
     8161 atcctgattc aatttttctt tatattcaaa aacaggatca aatgtcccat cttctatata
     8221 cgtatctact gtttgccaag ttcctgccca atctgaaaga gagcgatctt gtacatcctc
     8281 gtcttcgaaa taaccgttat agacgctctt ttcttttttg ggggtcaaag tatcttctaa
     8341 cgggttttct gtctctgtcg tttttttcaa cgcttcgagg tttgcttcca tgatagaaag
     8401 atagttttct ccattttcta tttgttcatt cgtcaatcct tccaaaggat tcaatacttc
     8461 taaagataca cctgcttcat ttgctaacgt tctggcaata ctatctttgg cgttttcttc
     8521 aaaataaata tagttgattc catgttcttt aacaaagtgt ttcagttcag cgattctcgc
     8581 cgcagacggt tcttcactag aagaaagacc agcaatcgat acttgattca atccatattc
     8641 taatgccaga tagttgaatg cagcatgttg cgtgataaaa gatctttgtt ttgcttgctt
     8701 aaaggtttct tgataaagct gatggagtgc ttgaagtttc tcggtatagg ctgctgcatt
     8761 ttttgtccat acttcctgtt tttctgggta agcttcaatc agctgatcac gtatgcttgc
     8821 tacctgcttg attgctaaac ttggtgctag ccatacatgt gggtctagct cgtgtgtatg
     8881 gccttcttcc ccgtgttcat ggtcatgatc atgtccttct tcacttcccg gaagtaaaag
     8941 cataccttct gtcgctttta tcgtatggac attgccttct tgtaatgttt tttggattgc
     9001 ggggacccat gtttccatgt tttcatcatt ataaacaaag atatctgtat cttggatctt
     9061 tgccatgtct ttagcagaag gttcataatc gtgggaatca gtccctgctg gaatcaataa
     9121 ctctacttct ccttcatccc cgacgattgc ttttgtaaaa tcatacatgg gataaaaggt
     9181 cgtcatcacg ctcaattttt ctttactgtc ctcggctttc ccacttgccc cgcaagcagt
     9241 taacatacct gctaaaacca gcatggccaa tatacctatc aatcgtttca tttatgacac
     9301 tcctcttctc atcttctttc atttgaatgt tccgattcgc cttttgtaaa ccgtaatagt
     9361 tacgttttag caacaggatt taatttatca tatggttaaa ataactgtca agcgttttct
     9421 tattttaatt gatgagaaag tttatgtgaa tgattgacga tatttttgca atcaacctct
     9481 atcattttcc ttttataccc gcttttgtct ataatgaagt tatagataaa ggaggaaaaa
     9541 gattgaaaaa agaaacagat acaacgaatc gtttgatccg ctttttagga ggaaaaacct
     9601 cttactatgt tttaggaata gttatcttac ttgcgctgat catttatttt ttccatcaga
     9661 tctcgtttat attcaagcct gtcggaatca ttatcgctac tatcttgccc ccattattgt
     9721 ttgcactgat cttgtattat cttttggaac cagtcatcaa acgtttgagc aagcaccttt
     9781 cgaggaactt gtcagtcatt atcgtttatg tgattatttt atttttattg gggcttggaa
     9841 gcgtatggct tgttcctttt cttgaagaac aggcaaagac attgatcagt agtttgccag
     9901 atttgtttca agattttcaa gagagtctcc gacaattttt ggagaaaaca ccttttgccg
     9961 attcatttaa tcaatttttc gcttctattg acgacgtgac gtctgatata gctggattta
    10021 ttggaaatta ttgggaaagt ggcgcacaac ggatcagcaa cattttttca actgttacag
    10081 ctatttttat tactttattt actggtccaa ttgtcgcttt tttccttttg agagatcctc
    10141 aaaaatttta tcagtcagtt ttagcgattg tcccaccagc atttcgtaca gattttaaaa
    10201 atttgacaaa gattgctaat caacaattgg gttcatttct aaaggggcaa atcattgctt
    10261 catttatcct tggagcagtc tactgggttt gtttcttact gatcggatta gaatttgcta
    10321 gtgttttagc tatttctgct gggttgttat gtatcattcc atatattggg ccgtttatcg
    10381 ccttctttcc aggtttgatc attgcattcc aagactctac ttttatggct gtcaaatttg
    10441 tcattgtctg gtttgccgta caacttctcc acggtgactt ggtgattcct cgagtaatgg
    10501 gtgatcgttt acagatccat ccgattacga tcttaatcgt tttacttgtc atgggtgatc
    10561 taatgggaat tgtgggcgtt atttttggta ttcctatcta tacgttagtc aaattactcg
    10621 ttacttttat tttccgtaaa ttcaaacaac gctacaataa attttatggc gataaaggag
    10681 agtatcaaaa ctcttcattt tctgaagaag attattttaa ataaaacaca aaacgtcgat
    10741 cttacgagaa tagctcgtag gaacgacgtt ttgtgttttt gttttaattt ttcagcaaag
    10801 caattgcctg gtatggtttt agctccagac tttgatgaag aacagtatcg gaataattat
    10861 cgataagaat attggcttct gtgaatcctt ctgggatggc gatccttgta tctttatcat
    10921 agaaatttgt taatacaagc agctgctgtc cttcaaattg gcgaagaaag ccataaactt
    10981 ccggatgatt tgcttcataa gcatgataat ctccttgtgc aacgattgga tatgtttttc
    11041 tcagccgaat caatttcttg taataggaca aaatagaatc ttcagaagat agttcttttt
    11101 ctacattaat ctcttctagc ttgcctccaa cttttaacca aggtttccct gtagtaaatc
    11161 ctgcttgttc tgtgtctgac cattgcatgg gtgttcgaga gttatcgcga gattttgctt
    11221 tgatccgacg aaacgcttct ttttctgtga atccttgttc aaggagtaac ttataagcat
    11281 tcaaactttc tacgtcaata tagtcggaaa tcgatgaata gtctgggtcg atcatgccga
    11341 tttcttctcc catataaata tagggcgtcc cacggttcaa atggatcatg gttgctagca
    11401 tctctgctcc ttgttttcga tactgatcga tagctacaaa tcgattgagc gcacgtggct
    11461 gatcatgatt attaaaaaat agagcattcc agccgttacc ctcactcatc ttttctcccc
    11521 atgtatgaaa tagtcgcttt aattctgcaa aatcaaatgg aggagtagac catttattcc
    11581 cattttcata atcgactttc aaatgatgga aattaaagac cattgataat tcttctctac
    11641 taggttctgt atacaagata caattttcga tcgtcgtaga gctcatttca ccgactgtga
    11701 tgatagtgga atccgtccca aacgttgctt ggttcaattc ttttagatac gtatgggtga
    11761 ttggtttgtc cgtatattca ggtttgccat cattttcttt attatttttc aattgttcgt
    11821 ctttaccgat aacattgata acatcgaaac ggaatccttt tacccctttt tccatccaaa
    11881 aacgaacaac tttgaatagt tcctctctta ctgcttggtt ccgccagttc aagtcagctt
    11941 gtgtccgatc atataagtgc aaataatatt tccccgtatc tccaaatggc gaccaagcac
    12001 ttccaccaaa ttttgatacc caatccgttg gctcttctct taaaatgaag aagtcctgat
    12061 aatgctggtc ccctgctagt gcttttttga accattcatg ttctgtcgaa acgtgattca
    12121 gcaccatatc gagcatgatt tctatccggt atttctttgc ttcggctatt agctgttcga
    12181 attcctccat cgtaccaaag ataggatcga tggctgtgta atcagagata tcataaccgt
    12241 tgtctttttg agggcttgga taaatagggt ttagccaaat catgtcgatg cctagatcat
    12301 tcagatacgg gagtttttct ttgattcctt ttaaatctcc aattccatca ccattactgt
    12361 ctttgtaaga tttgggatag atttggtaaa tgactttgtc tttaaatgtc atggatattt
    12421 acctccattc aactgattta ttacctacca aaacttatat atacaagtta tcatttatta
    12481 ttataactga accgaaagcg tttgtaaaat ttatctttat acattctaag aggtctgttg
    12541 cccaataaaa aacaagagat cagacatgta tttgtctatc ctcttgttta aaatagcaaa
    12601 atattcttgt ttcttctata aatggtgctc aaaagctttt atctacggca atcagtcttc
    12661 tcaaatgtaa aacatgagga gctaatttta tttgagtatt ctttttgctc gatgcaggac
    12721 agctttttca caacctctga ttcacggtgt tcaattagca gctgaccctc gttattagct
    12781 caaaacgctg atttcacaac ctcttcctta agcggtgttt aaaagcttgc atctgcggta
    12841 atacgcccaa tcaaacgaaa aatatgagaa gctatttttg tttggctgtt cttattactt
    12901 gatgcaggac agctttttca caacctctgt ttttattcat tcgatttcaa cgcatcaatc
    12961 atatcgatat gctttaattt gaaatgggta acgatcatca caataaccgt gaatacgata
    13021 gttaatcctg ctgataagac ataagcccac catgtgatca ctaatggaaa gatgacattc
    13081 tccatagacg cttgttgcaa gatgaagtca gtcaatatat atcctacacc aaatcctccg
    13141 atgattccta aaagagtaaa gatgatattt tctcgaacaa tgtacatcgt gacttcttta
    13201 tcaaaaaatc caaggacttt tattgtagat aattcgcgta cacgctcgga aatattgata
    13261 ttagttagat tatacaagac gataaaagcc agcagtcctg ataatacgac aaagattaag
    13321 acaaccgagt ctaagttcgc cattgattct tcttgcgttt cgatttgttt ggacatgaaa
    13381 gacgtatttg tcacagcgtc tgtggcaagc agtttctcgg aaagcatttc ttcttctttt
    13441 gttgtcatct tatctgtacg cagcaaaaat gcattatcca tcatcgattg atcagtaact
    13501 gaagcataat attcagggga catataaata aagctaccta gatagttttt cacgatttct
    13561 tttacttcga ctttccattc tttttgatct tcatcataaa gcgaaatatg atctcctgta
    13621 gataagtcaa atagttctgc tgctttttgc gtaatagcga ttcctgtttt aggcaaggag
    13681 cgtgggttct cttcaagatc agttaaatgg ataaaacgat ccaaatcagc tgttttactt
    13741 ggtacgatca ctgtcagact ttgcgaagca tgtccttttt ttctcaattc tacggtttga
    13801 gtatgaatag ctaggatctc atttactttt tcttgcttct gcagcatatc cgctgctttt
    13861 tgacgactat cttccgtgga tgaatccaat gtaacgattg cttgatagtc aatgatcggt
    13921 ccaaattgtt tggctgagac agaacttaga gaatctttca atcccacgcc tgcaaccatc
    13981 agcccggcac atccagcaat tccgataatc gccataagca ttcttgactt atatcggaag
    14041 agatttcggt aactgatttt ttgattaaag ctaaggcgtg accacagcgg agtgatgtat
    14101 tcaagaaaaa tgcgtttccc tggcttcggt gctttcggtt gaagcaatgc agcaggccgt
    14161 tctcttaaat ctttgaataa aacgatcatt gcagcgccaa atgcggcaat gaagaatgcg
    14221 gcagctgctt gtaaaatcgg cggccagtca taaaaaataa gcgcactccc aatatcatac
    14281 cgctcgttag acagctcaaa aatgatcctt ggcaaaagat tcgttcctaa tatcgttcct
    14341 aaaacaatgc ctatccctga ggctagactt gcatagattg catatttttt tgcgatttca
    14401 actttcgtat aacctaacgc ctttaatgtc ccgatttccc tgcggttttc ttctaccata
    14461 cgtgtcattg tcgtaaaggt gatcaatgca gcaataaaga aaaagaaaac aggaaaaaca
    14521 ttggcaatcg cagcgatacg atcagacaga tcaccaaatt cttgaaaacc gacattttct
    14581 gaacgttcat tgtataggta tgatggttta gttaaatttt caattttttc tgcatttcga
    14641 ttcaattctt cctctgcaga ggccagttca cgttctttct caactaattg cctttcttgt
    14701 tcagcaagtt gagcttcttg ttgagataat gtttgaacag cgattgttcg ttgcgcttca
    14761 ggaagctgct ggatagttgc tgactgttgc gctaactgtt ttttagcatc ttctaaccgt
    14821 gttcttgctt gcgttgtttg tgctttgcca tccgctattt tttgctgtgc cggttctaat
    14881 tcttcgttgg ccgatttttt cagctcttct aagcgttctt cgggtcgatc ttgtaaatac
    14941 ttttctgcct tttcttgatt ttgctccatt ttatctttgt aagctttgct gtacgtattc
    15001 aaattctttg cgtttgtaaa atcaagatat aagacagaat aaatatctgt tttaaaaacg
    15061 gattcaggta aatagacaaa ataatcgacg ctaccatttc cgacgttcgc gtatcctctc
    15121 tccaaattgt tgatgaacaa tggagaacgg acaaagcccg tgatggtata ctctttttct
    15181 ttcaattcat ctgaatcaat ctgataaata tcaccgattt tatacccttc atcttccgct
    15241 ttttcatcca atacaagctg gtttggtttt tccgggagtt ttccatcagt taattgtagc
    15301 ttgttctgct tgttattttt gttataagaa aatatttgaa tcacttcgtt tttgccagta
    15361 ttggaataat agatctgttt tcctgtttct acttttgcat ctgggatctt ttctgcttga
    15421 gcaatatctt tttgtgtcaa gccaccagta gaaacgattt gcaaatcaga aagtcctgct
    15481 gtaccaacat agtgatccat cgttttttgc atgacaggtc cagcagtttt gacacctaca
    15541 aataaaagtg ttcctagcat gacaataagg atgattgcaa tgaatcgtcc tttagattgc
    15601 cgaatttcgc gaaaacctgc tttaagatat gttcttgttt tcataaaatc cccctaccat
    15661 acaatctcat caatggatga aggatgatca tttaattcaa cagaccttac tttagcatca
    15721 ttaatgtgaa tgactctatc agcgatgggc gctaaagcag aattatgtgt aatgatcaaa
    15781 acggtattgc cgtgttgtct gctagcgttt tgcaataatt tcaatacttg cttgcccgtt
    15841 tcaaaatcca gcgcgcctgt cggttcatca cataaaagaa gtttgggatt tttagctagt
    15901 gcacgagcaa ttgaaaccct ctgctgttcg cctcctgata attgggacgg aaagttattc
    15961 aatcgatgag ctaggcccac ttgcctcaat acctctacag gatcaagcgc atcaggagaa
    16021 acttccgttg ccaattccac attttctttt gctgttagat taggaacgag attgtagaat
    16081 tggaaaacga accctacatc tgttcgtcgg taagcagtaa gctgtttatc tgaaaattgc
    16141 gcaatatctg tgtcatcaat aatgatctga ccttcatcag gtgtatccat cccacctaaa
    16201 atattaagaa tcgttgactt tcctgctcca cttggaccaa gtataatcac taattcgcct
    16261 ttttcaattg aaaaagaaat atcgtcattt gctgtaatgg tcgtttctcc cattttgtat
    16321 cgtttgtatt cattttttac ttcgacatac ttcactctga ttcctccatt ttcacgatat
    16381 ttctcgctat atctatgcat agaatgcgtt ttctcacact tatttatttt accactgaat
    16441 ttctctagac taaagaaagg aatcctttat ttagttgaca aataaacgat ttatgattta
    16501 cttacataaa aaacattgtt atttgaaaga aggaacatca tgaatgattt aaaggaaaat
    16561 atgatttttg atttatcaaa aactgctttt gtcgcgatcg acatgcaaaa agggatcgta
    16621 aataacggta cactctctcc acacactgca caatctattc tgtcaaacaa tgatttgtta
    16681 gtaagaacat tgaaacgtac cgctgccttg aaagttttag tgaacgtaga catctccact
    16741 ttcccttatt tgagtcaaca aactgatatg ggtggaatga ctgcttctgt acctccagaa
    16801 ttcactgact tgttgttgaa ggattccttg aaagatacaa acaatatgtt gactatcaca
    16861 aaatacaatc caagtgcctt ttttggtact tctcttgatc ttcaacttcg ccggcgtgga
    16921 atcgaaacta tcattttaag cggtgtagcg acaacaaacg gtgtatatgc tactgcatta
    16981 gatgccttcc aacatgggta tcatgtcgta cttgctgaag atgcttgcag tgatcgtgac
    17041 aaagaatccc atcaattgtt cataaaaaag atatttccga aaactgcacg agtacgctca
    17101 acgaaacaaa taatcgaagc tatccaacaa agcaaataag tactggcaca cgtgattttc
    17161 atttacttct ttttgatttc atgggaaaat aaccacagga tttagatagg agtatgaaca
    17221 tggtgaaaaa aaagatcaca atcaaagaag tcgctaaact ttccggcgtt tcaatcacaa
    17281 ctgtctcctt gatattaaat ggaaatacgg agaaattcag tccgaaaact gttcagaaag
    17341 ttttagctgc caaagaagaa ttaggctatc agcctgacta ttttgctaga caaatgatta
    17401 ccaaggaaac gaaaacaatt ggtgtactcg ttcctgatat cacgaatcca ttcttcagta
    17461 cactgatgcg aggaattgag gatatcttgt ataagcagaa tttcgtcacg atattatgta
    17521 atgctgattc tgatcatcaa aaagaaatcg aatatctagc cgagctcaca cgtcgaggtg
    17581 tggatggatt cattatcgcg acttctgctg tctctacaga tgcaatcaac gaaaatttga
    17641 aaaaacaagg ccggcccttc attgttttgg atcagaaaaa atctgaagga tttagtgatg
    17701 ccgtacgaac agacgatttt cgcggtggtt acctagcggg catgcacttg cttagtctag
    17761 gtcatcaaac gatcgccctt gtctatcctg aaaatccgcc ggaaaatgtc cacgctcgga
    17821 ttgaggggtt taaatctgct cttgatgttt accaaatctc tcacgaccaa ctgatcctct
    17881 tacctacaca gttctcaaaa caaggtggct accaaatcac agcagaactt cttgattctg
    17941 ctgcaactgg tgtgtttgct ttaaatgatg aattagcttt tggattatat cgcggattag
    18001 aggaatctgg aaaaagcatc cctgaagact acagcattat tggctatgat aatatagata
    18061 tgtgcgagta tatcaagccg aaactgacaa cgatcgcaca accgatcttc gaattgggac
    18121 aaacatctgc caaattattg ttagatcgga tccaattccc agaaaaagaa tgggaagaaa
    18181 aaagattacc tgtacgatta gaaaaaagat tttcaactgc acccttgaaa tagctttcaa
    18241 ggtgtgctat atttttatcg aactattaaa acgttttact aaaacgtttt aaaaaataat
    18301 ttttcccatc aaaataaatg agatagttgg aggtttgtaa aatgaacacg gttacagtaa
    18361 ttggaagtat taatatggac actacccttc gtttaaccaa catgcccaag ccaggcgaaa
    18421 cgatgcatgc gcatgagatt tttcacgctg gcggaggtaa aggcgccaac caagcagtgg
    18481 cagccaaacg ttcaggcgca cggacatctt ttattggagg cgttggggct gacagcgaag
    18541 gacaacagtt attagatcta ctgacaaaag aaaacatcga tacatctggg atagctgaaa
    18601 tccaaggcgc cacaaccgga caagcaatga ttatggtaga cgcagcagga gaaaatagta
    18661 tcttgatcca tgcaggggca aataatgctt tccatgaaca agaagtccta aaaaacaagc
    18721 aactgattac aaacagtgac tttattattg ctcagtttga aagtagctta gatgcgacga
    18781 tcctagcttt ttctattgca aaagacgcag gaaaaacaac gatcctgaat cctgcaccag
    18841 ctcgtgaaac gattcctact gaactacttg aaaagacaga tatcattata ccaaatgaaa
    18901 cagaaacaga aatcattaca ggaatcagag tgacagatca taacagtttg gtcgcagcag
    18961 ccgaaaaact ccatgaatta ggtattggaa cagtaatcat tacactaggc agtgccggcg
    19021 ctttctatca tacagaaaaa gaacacggta tcgtccctgc ttttaaagtg gatgccgttg
    19081 acacaacagc cgcaggtgat acatttattg gcgcattaag cagcacactt cagccagacc
    19141 ttagtaactt gaaagaagcg atcctttatg gaaatctagc ctcttctgtt gctgtgcagt
    19201 cttatggtgc gcaaccttct atcccttacc gcgaagcttt agaacaatca gcagtctaaa
    19261 attaacaatg agaggaagaa acaaacatga aaaaaggaaa agtaatcaat tcagatattt
    19321 cacgagtaat cgcacaaatg gggcactttg ataaattaag tatcggggat gcaggcatgc
    19381 ctgtcccctt tggtactgaa aaaatagatt tggctgtctc aaacggtctt ccaagctttt
    19441 tatctgtttt agaaaacgtt ttagaagaac ttgaggtaca atcgattgaa ttggcggaag
    19501 aaataaagac aatgaatccg gaaatattgg aaaaaatcaa attattgtta ccagatacac
    19561 cgattagctt tatcccacat gaagaaatga aaaaagagtt gaataactgt catgcattta
    19621 ttcgtacagg cgaaatgaca ccttacgcca atattatatt agtgagtgga gtggtcttct
    19681 aatggaaacg acaatgaatc ctgaacagat ggtactgtac gagcagattc atatgctgat
    19741 tgaacagttg ggaggaaaag acaatattga ttctgtcctt ccatgtgcga ctcggcttcg
    19801 tatcaaattg aaaaaacagc cagtagccaa tgcagaaaaa ttaaaacagc aaatcccttc
    19861 cattaagtat ttcattttac tcgatagata tgcagaaata atcatggatc agggattggc
    19921 tcctgcttac aaggaattga cgcgtcaaat cgatcattca gctccgaaag aaaagctaaa
    19981 tccgatcgaa aaactatttg gtttgatatc ggaaatcttt acgccgatca ttccgctgtt
    20041 tgccggttct ggtatattaa aaggtctggt tgttttagcg actacgattg gctggcttag
    20101 tgaaacaagc ggaacttatc atattttgtc tgctgcgtcg aatgccgtct tttattttct
    20161 gccaatcttt cttgcttttt ctgccgccaa aacgtttaaa gtgaatggat atattgcggc
    20221 tgtcattgct gcagcactga ttgaaccgaa tctgacctct ttactttcag acagtggcaa
    20281 agcagctact gattttctgg ggattcctgt agtactgatg cagtatacgt ctactgtcat
    20341 gccggcgatt ttaggtattt tcttttattc ttttgttgaa aaattcctca aaaaatatat
    20401 tcgcgataat atgcaattag tatttgtacc tttactttca ttagttatca cggttccttt
    20461 gacgttaatg gtattcgggc ctgcgggtgt ttaccttggc gaaggtttag ctgccgcaat
    20521 tacttggatg atggatatta acggaccgat cagcggcgca atcatcgctg gtggttggaa
    20581 tattttggta atatttggta tccaatgggc tgtaaaccct gtcatgatca gtaatatctc
    20641 tgcttatggg ttcgaccgaa tcgttccgtt gacaggagca gccaattttg gaatggctgg
    20701 tgcagcttta ggtgtatttc tacgaagtaa acgttcaaaa actcgttcga tctccggttc
    20761 tgcttttgct tcgattttac ttgcgggagt gaccgaacca actgtttacg gaattgcgat
    20821 cccattgaaa aaaccatttg ttgctgcttg cattggtgca gctgctggtg gagctgtaat
    20881 gggatttgcc caagtgaaag cgatcgcttt tgtatttggt agtctgacca cattgcctgc
    20941 attcatttcc ggtacttttt tctggtactt agcaggactg gctgttagtc tagtcgtagc
    21001 aatgattact actctcgtta gcgggtttga tgaagaccta atgtcttatg aatagttaat
    21061 gaaaaagact gaagtatgag atgtttatca catatttcag tctttttctt tttaggatca
    21121 ttatttttat aaatttttgt gatactttcc aaactttggt ataatttaca tatgcataac
    21181 catttttatc cccacttgtt tatccccaaa caaaggtggt ctatgcaata aaatgaacgt
    21241 caaagaaaaa cgaatgaatg ctcgttgctt atttacaaat tgttttattt agaatacaca
    21301 gacagattta tgaaaagaaa aagcttttcg acacgattat ctgttccaaa aaatgtctaa
    21361 tgaatgctga ataagacaga aaatcaatga aaggaaaagg actagtttat tatgaaaaat
    21421 aaaaaaacga tacgtttgct agctgctact tggctgattg ctccgtccgt tttgtcgacc
    21481 aataccgtat ttgccgtaga taatcaaaca gcagatcaaa cagtcacaac agatacgact
    21541 acatccagct cagctactag tgaaacaacg gattctttta cagaagaccc aactggaaca
    21601 actaactcaa caacaggatc gaccgagcca actgaaattg atccggctga tccatcagaa
    21661 aatgaaaagc cagccgagac atattccctt acaattgctc cttctttgat tggtaagatc
    21721 caactgactg cttcgacagc ggattcccct gaacagcaac ctttggatat tacggaaagt
    21781 ggaacggtag aaggtttatt agcggaaacg aaggtaactt acactattac gccagctgaa
    21841 ggtgaacaaa ttgcttcagt cgctgttaat ggtgccaaag aagtaaatta tgatgcaaag
    21901 acctttacag taggtacgaa caatttatct attactgcag aatttgaacc tgtaccggat
    21961 acttcaacag agaaaccaac tgatcctagt gaagaagtcc ctagtacacc agatccggaa
    22021 acgccaaaca aaccagatgt gggtaataac aacaataaca acaataataa tggttcaaat
    22081 aattcaaaca ataataacaa taattcgaac aacgggaatt cttcgaatca aacaaaccca
    22141 tctggatcga ataacaatgg aaatactcag agagaaccaa accatccaaa caatggttca
    22201 tcgaatggcg gaacgaacaa tgcttcatct cgtccaaata cagcaaatca aagcggtata
    22261 gagaatccat cttctgcttc ttctgatttt gttgtaaaat caccaattga tgctgtattg
    22321 cctacaaata caacgaatgt ccaacaagca atcgttcgag aagcattcga gcatcttgga
    22381 aaaccatatg tatggggagc caaaggtcca aatacttttg actgttctgg tttgacttat
    22441 tacgtatata tgaaagcaac cggtcattat atcggtggct ggacaggcga acagcaatat
    22501 gcaggtacgc agattccagt aagtcaggcc caacctggcg atctcgtctt ctggggacct
    22561 tcttccggtg ttactcacca tgtaggtatc tatattggga acggacaatt tatccatgcg
    22621 ccccaaccag gagataaagt tcgagtaact tctatttcag atttcacgcc tgattttgct
    22681 gtccgagtaa acttagcagg tttacctgct acttcaggct cgctaactgg ttcatctatt
    22741 ttagatggat tagatggctc gttccatttc agtcaaaacc aaacaactga tcagttcttg
    22801 aaaaagattg cagatgatgc acaggaaatc gggcaaaaag aaggaatcta cgcatcagtc
    22861 atgatggctc aagctatcct tgaaagtgga tctggaaaca gcttattatc aagcgaacca
    22921 aatcataacc tttttggtat caaaggatca tataatggaa gcagcgtgac cttcaataca
    22981 catgaacaag atagttccgg acaaagctac caaatccgcg cacaattccg taaatatcct
    23041 tcttacaagg aaagtttgga agactatgcc gacttgatca aaaatggact gactggcaat
    23101 cctgatttct acaaaccaac ttggaagtcg gaaacaaaag actacaaaga agcaaccaaa
    23161 tatttggaag gacgttatgc tacagatcgc caatattcac aaaaattgaa cgcgatcatc
    23221 gaagcgtacg acctgacaaa atatgatgaa ccgaaaaaag aagcagcaac aactgaagaa
    23281 gcaaacgccg aaatcctcaa tatgtctaag cgattcgatg tgccaatcaa atggacaaca
    23341 gatggcgtgc caatcacagg attagaattt tcaacgataa aacgcgtcaa caacgcttct
    23401 tccatcttcc gtgtcgcttc aatctgggat ttgtggaaca attttacggc aagacagata
    23461 ccagaaacaa ctactcgtac agttaaagct gcaactgttc ctgtactaac cttgttaagt
    23521 ttagacaaat cattctggct tgatctgcct ttaaagaaat agaataaatt aaaaagggga
    23581 tgtgacactg tcacactccc tttttaattt attctatttc tttatattta ttttgtattc
    23641 cgttacaaac tcgcgtactt gattaggttc cagagtgaca tctccaaaac tttcaagtcg
    23701 ctccgctcct ggcagttctt gagtctcaaa tgtgataccg ccatgattta ctaatttatt
    23761 cccgcgcatt tccggtccat cttctccaaa atttgctgta aaaatcacga ttcctgaagc
    23821 atctgttttt acttggatcg atactttttc atctaggctt gtcaattccg cagcatttag
    23881 taaaccagat tgttttttca acaaaaatgg atgatcgata ccgcccacta tcattgattg
    23941 gcgaaaatca ctggcaaaga cctcacctaa ttttttagga cgttggaagt caaatgctgt
    24001 gccacttact gaaactactt ctcctgtagg cgttgtatct ttattgagcg tagcaaataa
    24061 tcggctattc aaccaaagta tatgttcgtc gattgactgt gctggatcac cggttaaatt
    24121 gaaatagaca tgatttgtag gattgaatac tgttttgcgg tcactgatcc cttggaccgt
    24181 cactcgccat gtatcatcgt tcgtcagcgt ataccgtact tctacatcta aagttccagg
    24241 aaatccgttt tccatgtctg ggctagtctg atagaaaata accgatgctt cgttttcacc
    24301 attgatgact gtataatgcc actgcttggc ttcaaagcta ggtgcaccac catgtaaaca
    24361 atgaccagtg tccgggtctg tttttacttc gaacgtttca tcatctaatt tgaatgtgcc
    24421 attcgtgatc cgtccagcca tacgtccgat tgaagcaccg atataagggt ctttttctag
    24481 atattctttt gcttcatcaa aacctaagat caactctcgt ttttcattat cgattattgt
    24541 ttgaaaatga acaacccgtg ccccaagatc actgacagac aaagatgtcc cattttgatt
    24601 cgtgatagta atcaagtgat agttttcgcc aaaattttgt tcattgattt ccatgttcct
    24661 tgcaccctct tttctttcat ttgcactgat tccagtatac atagaaaccg ttttcttaac
    24721 agcttattcc gtgagtaacg agaaaaaatt tactatattc taataaaaga ccgcgaaaaa
    24781 aggttgtgac aaaagtattg tcacaacctc tttttccgaa caaacggtgt tcaaaagctt
    24841 gcatctgcgg taataagccc aatcaaacga aaaatatgag gagctatttt tgcttggctg
    24901 ttcttattac ttgatgcagg acagcttttt cacaatctct ttttcaatac gattccagta
    24961 tataaccatg tcgtcagtac atagtagatg aaatacaata cgaagaagat cgtaaacgga
    25021 acccaaatat ctttgtatgg attttgcatg aatagaccaa acatttgtaa tccgaataag
    25081 acatggacta cacctaagat tcctggtgct aagaataaca cgcctacttc tctacggatc
    25141 gattgtttaa gcaacccgcg tcgtgttcct atcttttcaa gcatttcata acgagcaatg
    25201 tcactcttgc ttcctgacaa gatcttgaac atcaaacagc tagcaagcat ggtcaagaaa
    25261 gctaaaccta ggaagaatcc cataaattca aaaccagaga atagaccatt atacacttca
    25321 tacatttcat atttctggtt gctgtactct actttttgca aagatggatt gttcatttta
    25381 ttttgagcaa caagtgtttc gattggtttc aggtttgctt ggaagtcttt tacctgaact
    25441 agctgtaaag tggtttgtgg caagttcagt tgatcaaatt ctgattgact gactaccttg
    25501 atttcttttt cttgctgttc cggcaattca tagcttcgaa gttcctcttg tacagtcagt
    25561 tcattcattt tttcagcagg aactttttct ttcgtgatct tcggcggtgt tccatcttca
    25621 tgttttatca tgatcaatgg ttgagccgca aattcacttg cattatagta aatcgttccc
    25681 gcatcttctt tttgagtata agaagcagaa agtgttggtg aaagttcttt gattttttgc
    25741 tgatcttctg cccgaggatt attcatcact agatcatatc cagaattgtt ttctgtcaag
    25801 tctgcaattt catttttaaa acctaatcct accgtcaaag cgcccagtgc caaggcaaac
    25861 agcatagcga ccattgagag catttgtgta tattcacgaa tacgaaagtt taactgtgac
    25921 aatgtgaaat tgttcaattt tttcaatgaa atctgttctg atttctttag taatgataaa
    25981 aagaaaatga tcacagaatg gaaaatgaaa taagttccta atacgatcgt aactaaggct
    26041 actcctatac caaagacttg ataggtcccc acgtgtttca tcatatagta accgcagcct
    26101 agaaaagcaa ttcccagtac tacttcaagg aaaaacaaga atttatgctg cttgattctt
    26161 gttggtgtct gatccgcacg aatcaatgct aaaatcggtt ttttgacaat cgaaaaagca
    26221 ttgacaacag cagctaataa aaacaaaaca gcaaaaaaca aaacagtgat caacaacccg
    26281 tgaatactaa aaggtgtaaa atgcgaaata ttgagatcta actgatccac taataatcgg
    26341 tggacgatac ttgtcaatcc tacaccgaca gctgaaccca ctaaagtagc tgcgatccct
    26401 actaagaatg tttcagaaaa aatcatctgt gcgatttttc tgccttttgc tcctagcatc
    26461 ataaacattg catagtcttt ttgccgcatc gtcatcaaaa aggaatttgc atataaaata
    26521 taaacgaatg tgatgatccc tagtaaaaca gaaccgaatc ggaaaatgat aactgtagcc
    26581 gcaacgatcg tattactttt caaaaaagct tcgttgcttg ccattgattc aaacatgtaa
    26641 aaaatagcag atgccataac taatcctgag aataacacca agtagtctct taaacggctc
    26701 ttgatcccag taagtgataa tttccaaagc atagtcaatc tccccctact gttccaagtt
    26761 tccaagaata gatagtattt ctcgatagaa ggaatcacgg ctttgatctc cacgtttcac
    26821 ttcttgatgg ataccgccat ccttgatgaa taaaatacgt tggcaataac ttgcagaaaa
    26881 cggatcatgc gtcacaagaa gaatcgatac atgatctttt gtattcaatt cgtccatcgt
    26941 atctaaaaga tcccgggcac ttttggaatc cagcgcacct gtaggctcat cccctaatag
    27001 aatcgttggt tgtgtgatca atgcacgtgc ggcggcgacc cgttgttttt gtccaccgga
    27061 aatctcagaa ggatagcttt ccaaaatatg agagatggat aaacgttctg caattttttg
    27121 gacacgttgc ttgatttctt ttggtctgac gccttgtaaa gaaagaggaa cagcgatatt
    27181 ttcttgtgct gtcaagcttt ctaataaatt aaagtcttgg aaaataaagc caatctcatt
    27241 cgcacgaaaa tctgctagtt gatttccttt taatgtggtc acatcttttt gattgattcg
    27301 gatatgccca tctgttggtt tatctaatgt ggataaaata ttcaaaagtg tcgatttacc
    27361 agaaccagaa gcgcccatga tcccaataaa ttcgcctttt tctacatcga agctgatgcc
    27421 gcttaatgct tgcgtctttt tctcattgtt ttttccgtat acttttgtta cattctgtac
    27481 ttcgactatt ttgttcattt ctttccctac cctttaatta gtttttccag tgctgttttc
    27541 ggcacgtctt ttttttcgac ttcttcatac gaagtgacac ctttattttt gttccaggtg
    27601 atttttaaat atgctccttt acgaagcggt cgagtcaatt gtcccttgaa gtccagttct
    27661 ttttccttac cctctttcgt atagccaggc aggatatatc tatagttgac tgtttcttgt
    27721 ccagagtctg attttgaaac tgtttttgta ccatctgtcg taatttgtac atagtattct
    27781 tctccaccca taactacgta atctgcgatt ttcacccctg caagtaccac aacgatgaaa
    27841 acaagtagac cgatcagaag ttttttcact ttttcccctt ctctcttact ttatatactg
    27901 attatagaaa aaagcgaaca attctgcctt cgaatcaact atcattcttc tttcaatttt
    27961 gtcattttct ttaaaacaac taaaaaaaga gttcaagaca ataatccgtc ctaaactcct
    28021 tcttcctatc ttacaaaaag tatgtcactt acggtctttc atgtcgcctg tattgataac
    28081 aggttgcgtt cctttgtctg taatgattga gacaagtaaa ttattgatca attggacttt
    28141 ccgatcatcg gtgaaattga tttcttgtcc atcttcgatt tgctctaagg ccatttgagt
    28201 gattgtgaca gctccttcaa caatggtttg tcgagcagac aaaatggctt ttgcttgttg
    28261 acgttggagc atggcactag cgatttctgt tgcataggct aaatggttca agcgtgtttc
    28321 gattacttcg acacctgcta ctgctaaacg ctcttgcagt tctttttgca actcgtcaga
    28381 cactgccgtc gtgtttcctc gaagcgtcaa atcattctca ttgaatgtat catacgggta
    28441 ttggctagcg atatgccgaa tggctgtctc actttggatt tctacaaaat cttgataata
    28501 cgctacatcg aataatgcct ttgctgtatc aacgacttta aagactacta ctgctgagat
    28561 ttcgatgggg tttccatcca aatcattgac ttttaaaaca gaactattga aattccgaac
    28621 tttcaacgaa acagtcattt tttgtgccaa aggtatcgta aggaaaaagc cattttctcg
    28681 aatcgtacct aaatattgtc caaaaaacaa aattactttt gcttgattag gactgactac
    28741 agttgccgaa cttaacaaca ataacgtgat caaccaaaga aaaatactta aaaaaagtgc
    28801 ccaaactttc gcttgccaca tgcctaaata gaagaaaaac aatccaacta aagctaatac
    28861 gatcaatccg acaatccctg cataaccatt gatataaaat gctctttttt ctctcatgct
    28921 acttcctgcg ctcctttatc cttcaataaa tatctaatgt tactttttta attttttttt
    28981 cgaatacgcg ccattacacc ttctagttct aactctttgt cttcagtgat gtaccaaccg
    29041 cttgcctgtg tccctccggc tgcctgcggt aaataatcca attttgttcg aacccagtgc
    29101 ttttcttctg taaaatagtc ttctaccaag acctctagat ccatccctgc tgaaagaggc
    29161 aaatcttccc gaacagattt aaagataaat cgttccttct tcccatcata cttcaatatt
    29221 cctgtatcga taatcggtaa agaataataa tccagctctt tcttcaccgt caataaatgt
    29281 ttgaaaactt catggtacac ttgctctaat tggcgacctt ctgaattgtt cgggtaatta
    29341 accatcactt tttctttgtc tggatcatag aaggtttggc ttagttgagt gatataagta
    29401 tgtaatcttt ccatttctgt ctttaacttt tctctttgac cttcttctgc cttcatatac
    29461 tttttcaccc tgttttcttt atagatattt tctttcttat tttactatag ttatgtgcgt
    29521 taagcgatga aaaacagcaa agattggaag gatctttgct gttttttctt aaaatatcta
    29581 gttgagtaca ggatcaatct gtttcattgt cctcttgatc cgtcgtaata ttgatcgatg
    29641 tgtctttagc agtgatcttt agctttttct gtccttgttc taacaaagta tttgttattg
    29701 gctggtagtc tttttcaata taagattcac cagacgtttg gatagtatag ccactgtttt
    29761 ccatctcatg caaagataaa ttaccaccgt ttaggatgac ttttccttct ttgctgatca
    29821 aactgtcttc tagatggaag ttgctgtttt cacctgtgac gatcaagtta tccacattgg
    29881 cctcatctag tcgaagtgag cctaattcat ctttcgcagt tattgttcct cctgtaaatt
    29941 cattgaacga tacccagcca tcatgtgtct tagtattcac ttttttgatt gatacttcgt
    30001 tgatatcaat tgcactatta ttcccttcga ttgtcaattc agataattca taatctgcag
    30061 ggacaacaat agtgatttgt gcacttgtgt cttctaaact aatgattgca cctgttgatt
    30121 ttttgactgc attcacagtc aacacgccat ttttctcctt tatttctggc ttttctgtct
    30181 tatcataagt cgtttttatc tcaaaatcca atccgcgtat gaccgaaact gtttgatttt
    30241 ctgcatggat gacgattttg tttgcatcct tgattttttt tgtctcagta accatatcga
    30301 tcaaccgagg accattatcc caagtcagac tcttttgagc gcctaatgta taagttgcac
    30361 ctgctaggag gccaccagta agcatcagac caataccggt aatcaaaaaa tgtttcaatc
    30421 tcaatttatg tgtcctcctt caaacgcccg ctgaatgcag atctcttttt gtaataaaac
    30481 gtcgtcccat ccatttaaca aagttcatca tgacataaaa aagccctttt gttcctttca
    30541 gcaacagtgg cgaaataaat actgcgcttc ctgtggcgaa cagaccgaca ccgataaaga
    30601 ataatccact cagaatcgat tgtccaaaca cacttattcc tgatatgatc atgaataggc
    30661 caacacctaa gatactgata cataaaacat agatactaaa aatgagcgct agtaaagcaa
    30721 ccaatagtcc cccaataacc aatataaacc ccagtgctag tggaatgagg acaggagaag
    30781 caaataaacc gataatgatt aaccaaatca ggcgaatttg tctttttgga tttttcacta
    30841 actcgttttc tgcttgttct tcttctgaaa agtaactgat ttttaatttt aaggcaaact
    30901 gctttggtgt accatattca tcaatgatct gatctaatgt ttttccagaa tctaaaaaat
    30961 attcctcata atactggatc aaatcaaaac gatcgtcttc aggaatttct tgaagatgat
    31021 ccacaacttc attcaaatat tcattcattt gtgctctcct ccaataaccg gtctaaagaa
    31081 tctcgaaaaa caatccattc atcaataatt tcttctaaat gacgacttcc cattggtgtc
    31141 aacgagtaat atcgacggtt tctgccttca aaaggttcat cataggtctc aagatatcct
    31201 tcttttttca atcttctcaa aaccggatat agtgtagatt cactgacaga gagactttct
    31261 tgtacgattt ttgttaaggc atacccatac atatcttcct tgcttaatgc tgctaacaca
    31321 gtaccatcaa gaagaacagt tggaatctgg atgtgcatta cgtcaccttc tattctcaag
    31381 tgtaatattt tatttcaatc tatattatac aacatataat atagtttcaa aatcagtttg
    31441 tcaaatacaa aaaaactgac taaaagattt gatccttttt agtcagcaaa aaaatggctt
    31501 ctctttgatt tttattataa cagtggcata atccaatttg tccacatcca gctcagtggc
    31561 atgataacga tcattgcagg tatcatgtca gctactggga acattttcac gttgatcatt
    31621 ctaaaaccgg tagcgaccat caagaatccg ccgcaagctt taaaatctaa gatcatgtct
    31681 ggtgtagtca atggataaat aaattttgcc aataagaaca aaatataaaa aataatgaat
    31741 tggggaattg ctatcacaga aacgacatac cctaaattac acgcaaaaat agctgctgtg
    31801 aaaaaatcaa ggacagattt agaaatcaaa atcgtactgt ctcctgtcat tccagaatct
    31861 aaacttccat agatccccgt accacttgca caaaatagaa caatgaccgt cactaatgta
    31921 ctgataaatt catcgtgggt cataccaagc ttttcactag ggaagatttt agaaataggt
    31981 acttgcatca gcgcagcccc cttatttatc ccattaccta aatgaacaat cagaccaatc
    32041 gctgttccta tcactaaagc aaaaataaca gccggcatat ttttcatcgg cgcaatggaa
    32101 tagattccca tccccatcga acacacacca aaaaccatcg tcagctgtgc tttaaaatgt
    32161 tcacttaatt tatttcctac taatccgcca attagtccgc caagcaaaac ggaacctaca
    32221 ttgatcaaaa ctccagttgg catcaaaaaa actcctctct ttttctacga agcgaaaaaa
    32281 atcagaaaaa tttctgtaat tattgctttc gctgcaagaa agattgtaac agacagagaa
    32341 gattacacca attcattttt tgtgttaaag tattctttat tggaataagt aggaggaata
    32401 tgatggatat ccataaattc gaagtttttc ttgatttagc cgaaacaatg aatttcacca
    32461 aaactgctga tcgacaattc accacccaag gaaatatttc taaacagatt ctttcgttag
    32521 aaacagaatt agacgtcaaa ttattcgaac gcgcccatag aaaaatcgaa ttgaccgaag
    32581 ctggaagctt attgctccca tatgtaaaaa atgtcgtaga acaataccat gctatgcaag
    32641 aagtgcttga aacctatacg aaagacaaca atctttcatt aaatatctta acgataccga
    32701 caatgatcaa ttataaaggt ttttccaaaa tcaccgactt tttgaaacgc catcctgaat
    32761 ttacagtcca gttgaaagaa gtggaaagtg tcgaattaac ttacaatgac gccaatgaac
    32821 atagtgacac gatccacttt gctagaagtt tccaggcccc gccgagttcg attgagtggt
    32881 tgcctactga aacagacgat tttgtagcag ttctacctaa aaatcatcct ttagctagta
    32941 aaaagaaatt ggatctaagc gaactgaaaa aggagaactt tttgctactt ggccccacta
    33001 cgaacttgta tcaacctgta ctagatctgt gtcgcgaagc tggatttgaa ccaaaaatct
    33061 cttataaagg agcaagaatc gacttgatca tcaatatgat tgcaaatgac atggggattg
    33121 ctattgttat ggaaaaaaca gtcaagaatc tattaaaaga aaatactgtg attctcccta
    33181 tttctccaac aacagagagt tatcttgcat ttactcgtaa agtcggtgag cattctttgg
    33241 cttcagatac attttgggag tatttaaatc aagaagaaaa ctaagcccta cattttctgt
    33301 ccatctatcc accccaaaaa agagagcgac gcatgacttt cgctctcttt cttacatcgt
    33361 gccattgatt caatgacaca tctaacgttt ctcgtatttc aatagaagat cagcgatttt
    33421 atgccggaag aacattaaga gcaattcgct aatcacaaaa aaacgtctaa aaatccctaa
    33481 aaaccttcac tagaattttt aggtattttg ttcttttttc ctattatttg cttaaacgta
    33541 ttattgctgt cgtccctact ccttcgatac tgctataatc gatacgtcca tcatgtagtt
    33601 ctagaatttt tttgaccagt gaaagcccta gtccattccc agaaatctga cggcttgtat
    33661 ctccttgata aaatttttca aacattcgat ctcgggtctc ttcattcatt cctacacctg
    33721 aatcggtcac ttccagtacg atctctgtgg ctgtctcaaa taatttgata tgaatctttc
    33781 cattgacttg attgtacttt atggcgttat ccatgatatt cagccacact tggtacaaaa
    33841 actcttcatt acctgtatag tttactgcag caagttcgat atccaattct agctgttctt
    33901 tttcccattt aggctgcaaa aaaacgatta cttctctgat ttgttcatct aatcgatact
    33961 ctttcttttc cagttggata cgctggtttt ccagtttgtt taattttaaa atgttctctg
    34021 ttaattgtga aagctgcgtg atactttcga tgacccgata taaaaatatt tggcgttctt
    34081 cgtcagaaag atttggggct tgtaacaatt gaacatatcc ttggattgtt gctagtggtg
    34141 ttttgaattc atgggataca ctggacacaa agtcattcct cagtgtctcg atactattga
    34201 gttcttggac cataacgttg aaatctttat acagttgctg tacttctgct actttctgct
    34261 gttcatccat tcgaatcgaa aaatctcccg ttgctaccag actcatattt gtccctagcg
    34321 cactgattgg atgcaggata cgccgcccaa caaaaatcga tattcctgta cccacgacca
    34381 tactgaaccc cgcaaagacg aataacgggt ataacgttgg ttttccagcc gattcagcag
    34441 gcgagataga atgtcgagac aataagaaaa taaaaccaag aaaacacagc agtgttacta
    34501 agatcgaaat aaatacgata ctagcaaaat agatccataa ttgtgaaaat agcttctttt
    34561 tcatctttaa ataaccgcct tatagcctag tccccgtact gtgatgattt gaaaatcttc
    34621 tgtcttttca aaacgggtcc ttaaacgttt gatatgtaca tcgatcgttc gttcttctgt
    34681 attcgtgtct aatccccaaa tatcgtccat caactgctgg cgagtgaaaa tcttgtttgg
    34741 ataagacagt aatttataca gaagcagaaa ttctttttga ggcagaataa aagaatgctg
    34801 attttgtacc actgtataag tatccagctc taattttgtt tgcccaatcg tcaagatact
    34861 gctgtaattg atttttgaac gtcgaagcaa tgcttccaca cgcaggatca tttcattgac
    34921 atcgatgggt tttgtcatat aatcatctgc acctaatttg aatccacgtt tcttgtcctc
    34981 gaaatcagcc tttgctgtaa tcatcagaac tggcatgtcg aatctggcgt tacgtaatga
    35041 tcctaccaag tcatagccat ccatttcagg catcatgaca tctgtaatga tcaattctat
    35101 atgtgtcgct tctagttttt tcagcgcatc ctctccattc agtgcggtga tgactgaaaa
    35161 gcccgctcgt tccaacacag aacggtaaag caactgtaat tgcggatcat cttctactac
    35221 taaaatacga ttcattgatc tccctctgtt ttttgaaaaa tttctagatt ttttcttgta
    35281 gttcatcttc agttaacatt tatttcgtat actacgatca ttgttctgat atacttatta
    35341 gtataccgaa tatttcaata cgggagaagg gtgattatgc ttcaattaaa agatataaaa
    35401 aaatattata aagtcggcga aaccaccacc aaagcattgg atggtgtgtc tgtcgctttc
    35461 agacaaaaag aattcgtagc gattttaggt cctagtggtt ctggtaaaac gactatgctg
    35521 aatgtgattg gtggactaga taattatgat tccggtgata tggtaatcaa cggaaagtct
    35581 actaaagact ttaaggacag tgactgggat gcctaccgaa acaactctat cggtttcatt
    35641 tttcaaagtt acaacttgat tggtcatcta ggcattattg aaaacgttga gcttgggatg
    35701 acattaagtg gtgtttctaa agacgaaaaa cgaaaacggg cagaagatgc attgcaccgg
    35761 gtcggcttga cagatcatat gcataaaaag ccaaatcagt tatctggtgg acaaatgcag
    35821 cgagtggcca tcgctcgagc actagccaat gacccagata tccttttgtg cgatgaacca
    35881 accggagcgt tagatacaga aacaagtatc cagatcatgg aattgatcca agagttatcg
    35941 aaagaaaaat tagtgatcat ggtcacacat aatcctgaac tggcccatca atacgcagat
    36001 cggatcattg aattttctga tggtaagatc ttaacagatt cccatcctca catcgaacgg
    36061 ccaaaagatg atcaatttaa cttacgcaga acaaagatga gtttttggac agcgttgaag
    36121 ctttccttca acaatatccg aacaaaaaaa ggacgtactt tcttgacctc ctttgcttcc
    36181 agtattggga tcatcggaat cgcgatcgtt ctgtcccttt cttctggatt ccaaaaacaa
    36241 atcgataaca cgcaagctga aacgatggcg aaatttccga ttactatttc taaggtaacg
    36301 actaaccaga ctcgtgacga tgccggattg ggtgcaagca aagcggatta tccagacagc
    36361 aaaacgatta ctgcaaaagt cagcgaagaa gataaagcac agcacacgaa taagattgat
    36421 caaacatacg tcgattacgt aacagacatc gatcctaact taagtaataa tatcggattt
    36481 acccgtacta caggaatcaa tcttttacgt gatgtaaatg gcaaagtaca gcctgtcagc
    36541 ttctcgaatc aaaatcctga tacggaatct ctttctttat ctagtacgat gtcagcaatg
    36601 acaggtgtgg gagtgtcaag tttccctaca cagcttgaca ctagcaaaga aaacttttta
    36661 aaagacaatt attctttact tgccggaagt tatccagcct cagcaacaga tgtcgtgctg
    36721 atcgtagacg gcaataataa tactaatatc aacgcattga agaatcttgg atttgatgtg
    36781 aaagaagacg aaaaacttga ttttgatgat atcgttggta caacattcaa attagtaaac
    36841 aacaatactt actatacaaa acttccaact gggaatttta tccctaatac agattatgat
    36901 gcgatgtatc agaatgcttc tgatgaattg aagatctcgg ggatcctacg cgtgaaatct
    36961 tcttctacaa tgaatctctt atctccagga atcgcttata gcgatcaatt aacgactcaa
    37021 atcgtcaacg aaaacaaaga atcagaaatc gtcaaagctc aaagagacag tgatgtaaat
    37081 gtcttaacta ctgaaaaagt tgacgaaaat gccaaacaaa cattgcttag ttatttaggt
    37141 ggagacagct tgccttcaag tatcatgatt tatccgaaca attttgaaga caaagaaaag
    37201 atcttggatt atctagatga ttacaataaa ggcaaatcag atgaagataa aatcatttat
    37261 actgatttag ctggaacgat gactgaactg actggcggat tgatggatgc tatcacttat
    37321 gtccttattg cttttgcagg gatctcttta gtaacaagta tgatcatgat cagtatcatt
    37381 acttatacat ctgtgattga acggacaaaa gaaatcggtg tactaaaagc tttaggtgca
    37441 cgtaaaaaag acatcactcg cgtatttgac gcagaaacgt gtattttagg tatttcttct
    37501 gggatattag gtgtgtttat tgcatggcta gctacattcc ctatcaatag catcttatac
    37561 aacatgacgg atctaaaaaa tgttgctcaa ctaaatcctg tccatgcgat tattctagtg
    37621 atcgtctcta ctatattgac gatgcttggc ggtcatcttc ctgcacgaat ggcagcgaaa
    37681 aaagacgccg ctatcgcttt gcgtgcagaa taaggttgtg acaaccacat ttagatttga
    37741 gtcataagag cgctgaaata gtagaaaaca gcaagaaacg aggaagtaat atgttccttg
    37801 tttcgcgctg ttttttttaa ttatttttta taattctggt atacttttct ttcctttgta
    37861 ccacaactct tctggcgtat acctgtcttt tccatttatg aatactgttc catctgcttc
    37921 aatcgttact tcagtcccaa tccacatatt acgagaaagt gcgagaagat tttttctttt
    37981 tcctcttcct ttttttccaa attgcaagtc atcggttatc tcttctgtcc aatgagattt
    38041 cacgtatttt acttctccgt tttcaaatgt aactttcaaa atattcgcta tattttccac
    38101 ttttgctgct ttaggaaatg actcctccat ttttttcact ttccttcctc caacactaag
    38161 tatgcgcttt cttttattga taagtatatc atagtttatt ctattatcaa aaaaacgcat
    38221 ccattccatt gtagaatact ttaccgaaca agttgaattg tttttctctt tttcaccttt
    38281 tactatagat aggtaaagac aactaaggag gaatctctca tgacagatca accgtatgat
    38341 ttaagaaaag tcatcgaaga actaaaaaca cttccaggtc aatatcacga aacagatatt
    38401 gaaatcgatc ctgaagcaga attatctggt gtttaccgtt atatcggtgc tggcggtaca
    38461 gtaaaacgtc cgactcaaga aggaccagct atgactttca acaatatcaa aggtttcccc
    38521 ggcacacgtg tgaacatcgg aacaatggct agtcgtaaac gtgtaggtga aattttgcac
    38581 catgattatc gaacacttgg tcgtttcttg aaagacgctg tagaaaatcc aatcaaacct
    38641 gtgaaagtag acaaagcaga cgctccagca caagaagtcg ttcatctagc taccgatgca
    38701 gacttcgata ttcgcaacat tttgcctgca cctacaaata ctcctgaaga tgctggtcca
    38761 tacatcacaa tgggtgtagt cttaggatca gatccagaaa aaacaatgag cgatgtaacg
    38821 atccatcgta tggttctaga agataaagat acgatcggta tgtacatcat gcctggcgga
    38881 cgccacattg gtcacttcca aaaacaattc gaagccttga ataaaccaat gcctatcact
    38941 atcaatattg gtttagatcc agcaattgcc attggcacga ctttcgaacc acctaccact
    39001 ccacttggct acaatgaact atggtgtgca ggagctttgc gtaacgaacc tattcaaatc
    39061 gttgatggta tagcagtcga tgaagtgggt attgctcgtg ctgaattcat cattgaagca
    39121 gaaatcatgc caaatgaaac aatgcaagaa gatatcaaca cgaactcagg aaaagcaatg
    39181 ccagaatttc ctggctatac tggggatgct aatccagctg tacaagtagt gaaagtcaaa
    39241 gcagttactc accgaaaaga caatccaatc atgcaaacaa cgattggtcc cagtgaagaa
    39301 cacgtatcta tggccggtat tcctacagaa gcaagtattc ttgccttggt cgataaagct
    39361 attccaggaa aagtagtaaa tgtctacaat cctcctgcag gtggtggtaa attgatgacc
    39421 atcatgcaaa tccgtaaaga aaacgaagcg gatgaaggta ttcagcgtca agcagctatt
    39481 cttgctttat cagccttcaa agaattgaag acagtcattt tagtcgatga agatgttgat
    39541 attttcgaca tgaacgatgt agtttggact ttcaacactc gtttccaagg agataaagac
    39601 gtcattgtga tcccaggaat gagaaaccat ccattggacc catcagaatt accagaatat
    39661 aatcctggct ctattcgttt ccgtgggatg agtgcaaaaa ctatctttga cggtacagtt
    39721 cctttcgata tgaaagaaca ttttgtacgt gcaaaattcc aagaagtaaa agattgggaa
    39781 aaatatttga aataatgaat gatctgcaac tgttttgaca tcataaaaag gagtgcggaa
    39841 aatggaacaa ttggcacaac ggaagaaaaa aatcgtgatt ggtgtaagtg gcgcttcagg
    39901 caccatttat gccatcaacc tagtgaagaa actaaaagaa tatccgatga tagaaacaca
    39961 cggaatcatc agtccatggg caaaacagaa cttaaagctg gaatccgact tatcactaaa
    40021 tgaattcgaa agcctgtttg attaccatta ttcaaacaaa gatttaggtg ctgctgtcgc
    40081 cagcggatca tttttgaccg atggaatgat catcgtacca gcaagtatga aaacagtcgc
    40141 aggtatctct gttggtttag gcgacaatct tattgcacgt gctgctgatg taacaatgaa
    40201 ggagcaacga aagttagtca ttgttcctcg tgaaacgcca ttaaatgcca ttcacttgga
    40261 aaacttgact aaattagcac gactaggtgt gcaaatcata ccaccaattc ctgcttttta
    40321 taatcagcct caaacgatcc aagatcttgt taatcaccat acaatgaaac ttttggatca
    40381 atttggaatt gtaaatgagc attcaaaacg ttggcagggt gattaatatg gaaaaagaaa
    40441 cgatagttga agtaaccaaa ctaggtttca cgatccaagc tgctgttaaa gtaatcggta
    40501 aagacttatt gatcacttta acaggcggag atacaccaca tatcggaaca gtcactacat
    40561 tttctcgtga tacagagatg caaactgtac gtttccctag ccacgatggt tggttccata
    40621 aagacgatgt cttagccgaa aaaatagctg tgatcatcaa aactgccgtt ccgggaagtt
    40681 gtacgattac ttccggtatc catgttgacc atatctccaa tgcacaaatc aacgcctctt
    40741 ttccgatgtc agaagagtta ggttacaaga ttttagattg gttggaaaaa atagatttcc
    40801 atatcaaaga accagtttat tataaagatg gtgaaaaacc attatgaaag aagaggttgt
    40861 gaaatcagcg ttatgacctg agtatgagag aaaaaaatgg aaaaatagct tctcatattt
    40921 tgccgttttt gagctaatac cgagggacag ctgattgaac gccgtttatc aagaggttgt
    40981 gataaagccg cttcgtttcg agtagtaaga aaaattttgt aaacatgggt ttccacattt
    41041 ttgcataaat ttgacttact accgagaaac tggcttttga acaccgttta tcaagaggtt
    41101 gtgacacgtc ttttgtcaca acctctttta tttttgcttc actgtaactg ctacaatatc
    41161 accgatttct ttaccgatgt ttttacgaat atcttttcgt agtccaagga tgtagcagac
    41221 tgaaccatct ggatttttca cacccatatt cacgatactg ccttcgtacg gcacattgtc
    41281 aaaagttgct tggactttaa ctcttccagc accgaattct tttcttatat catacggaaa
    41341 aataatatat gccccgccct tgccgacttc ggaagcatag atttttgatt caaaagaata
    41401 aatttccctc attttatttc tcctattttt tacatctagt atagcatagc aaaaaaaaga
    41461 aggaactgtg acaaaagtct tgtcgcagtt ccttttttcg aataaacggt gttctcctgc
    41521 caactcctcg gtcacaagtc acaatattca acaaacatga gaagctgttt tttgaaattg
    41581 ctccttgtga ctcagagtta aacggcaggc tcacaacctc ttccttaaac ggtgttcaaa
    41641 agcttgcatc tacggcacta accccaatca gatgaaaaat atggagtgct atttttatct
    41701 gattgttctt actgcttgat gcagagcagc tttttcacaa cctcctacac ggtgttcaaa
    41761 atattattcg ccttttgctt ctttcactaa ttttgcgaat gcttgttgtt ggaattcagc
    41821 agctaaggct ttcgtacgtt gttcttgctc tgctttgctc atttttggat tttcagtcat
    41881 tgcttttacc ataccgtctt gaacataagc ttttgcttca ctttccatcg tactttgctg
    41941 tttcttaact aattcagcta acttggctgc ttggtccgta cttaattcta cccatgtctc
    42001 tgggattttg aatgtattca ctcggctgtc atattcgtga ttgttatcag caatattgaa
    42061 ccagaattta tacatgtcgt ttttgtattc ccaataagta gttttcgtca ctttttcagc
    42121 atcgccgtct gtcttttcta ctttatttgt ttcgttatcc gtaccagttg cttttggttt
    42181 ttcttgtttt tcatttgttc ggtaaagata cactttttct tttccattcc ctaaagcttt
    42241 atacaaaagc atatctgctc cttgggaatc agcactggaa acaatttttt ctgtttttgt
    42301 ttccgttact ttttccattc caaagtgatt ggacaaatta gcgacgatca aacttagtga
    42361 agcgacgaag attagtccga aaatcaaaga taaaaaggtt tgccatgttt ttttagcgaa
    42421 cacgttcgtt aaagcaaaag caagcaagct gacaatcaaa actccaataa tcatgcatta
    42481 tcccccgctt tcgttagaac aggttctttt ttcttcaata aaaaggcaat aataaagcca
    42541 aggatcccaa agatgatcgc tacgataaac gcggcatggt aacctgacag tgtggcattg
    42601 attgcctgat ctttataagc taatggtgta ttatccaata cagatttgct tgggatattt
    42661 gcttttgtca cattcgtcaa cacactgatc aaaatagctg taccaatcga gcttgcgatt
    42721 tgacggaatg tattatttac cgctgtcccg tgactgatca agttatttgg caatgcattc
    42781 ataccagaag tcgttaccgg catcatgacc atggagatcc caaacatacg gatcgcataa
    42841 aggatcacga tatataaaac tggagtcgtt tttgtcaaga aagcaaatgg tgcagttgcg
    42901 gctgtcaaaa tgaacatccc tgtgatggct aaacgtttcg cgccatattt atcgaaaatc
    42961 gcacccgtaa caggcatcat agcccccatg atcaatgcac ccggcaacaa catcaagcca
    43021 gaatggaatg ctgattctcc acgaatattt tgaatataca atggtacgac catttctgcc
    43081 cctaccatcg ccatatttgt tacaccagct aaaacagcgg caatcgtgaa tgtagatgat
    43141 ttgaatacgc gaagttccaa gaatggatgt tccaaatgta attgacgcca tgaaaacaat
    43201 gcgatgaaaa tcacaccaac aactaagaaa ccaattactt tcaagcttcc ccagccgtca
    43261 tctcctacac tagaaaatcc gtaaagcaaa ctaccgaatc caatcgttga taaaatagcg
    43321 gataaaatat caatgctagg atttgacaat ggaataacgc tcttcattag gaagaatgct
    43381 aaaaccaaga caagaacaac gattggcagt accataccaa acaagtcacg ccaatggtag
    43441 ttatcaatga tataaccaga taatgtaggg cctaaagcag gagcaagtcc aataacgatc
    43501 cctgacatcc ccattgctga tcctcttttt tctggcggga aaatagatag catgatattt
    43561 tgcaataatg gcatcgatac tcccacaccg caagcttgga tcaagcgacc gattagtaat
    43621 gtactaaaat taggtgctgt aaagcaagta atcgttccga tcaaaaagat cgtcatggca
    43681 gaaagatata aatttcttga actgaattta ttgatcagcc atgctgttat tgggatcata
    43741 atcccgttga ctagcaagaa accagtcgtt aaccattgca catcagacgc tgaaatatcg
    43801 aaagctttca tcaatgtagg aaaagctgtc gtcaataagg tctgattcaa aacggtacaa
    43861 aatgtaccaa ttaataagac aacaactaaa agtgtccgat tatatggttt tccgtaaata
    43921 tctacgggtt ggttgttact catatttctc cttctttctg aattttttct gaaactacta
    43981 gaatacttta gtcctaaatt cgctatttgt caacctactt gacacataag ttaatacgga
    44041 tataattaag ggaagcaaga ggacaaaagg agtgacaaaa atggttgggg cttcccttaa
    44101 acgtttgttg gaaaatgatc aattggttgt tggcatcagt gaattaagtg atatcgtagg
    44161 tgtgtctcca cgtcaattaa ggtattggga acaaaaggga ttcattcgtt cgatcgaagc
    44221 tgatgcgaat tgtcctagaa aatatcgttt acctacagtc ataaaagtag aactgatcaa
    44281 aaaatattta gatgacggat ttactttgtc aaaagctgca gaaaaggcag aaatccgcca
    44341 aaagaaaatg caccatgtcc gtaaagtttt ttcaaaagca atcaaagata tcgaattgat
    44401 cgacgaacgc tatacgattt tacaaatcgg taacttcaaa gaagagaatg ctaagatgta
    44461 catcattcat gacaacgaga cagacgaatt aacttacaag ctccttcctg tcgatgaaaa
    44521 agctgactac gaacagctgt taaaagaact ctaaacgcca ctaatctcct aagtaccaag
    44581 ctttttaata agtagatgct acataaatac ttgaaaaaag gcctgtttga acataagaat
    44641 atccgaacgg tgatctgcgt ttctttcata tagaaaggaa ttctccatca ctgttcggat
    44701 atttttttat ttttttcggt tactttcttc taatacatct gttagccaat ttgcatattc
    44761 tgtttcccga ttgatcgcat gcgctaaaat caggtagtgt ccgtaatttt catgtttttc
    44821 tttttcagta ggaaagatcg ttttttttcg ctctaataga tgaagtcgtt tttcttcatg
    44881 aaggtcacgc tgctgctgaa taatttctac taatgccgga tcattcacgt ctttgacgaa
    44941 atacaatttc agaacaaatt cgtctcgatt aaccggtagt tcattggtgg gtgtatgaat
    45001 ccactctgcc aattgttcct tcccttcttc tgttaattca taaactttct tttccaattt
    45061 ttcacctgtg atctctactt gatgctgaat gattccttgc tcttccattt ttgcaagttc
    45121 aggataaatc tgactatgtt ttgcttgcca aaattctcct atttcatttt tgaacgattg
    45181 attcaattca tatcctgtca tcttcttctt acttaataac cctaacaaaa tatatcgaag
    45241 tgtatttttt cttgccatca gttttccctc cttgacaaat ctttaaatcc attctatcat
    45301 aaatgagtaa tcgaaaacat gtagtttatt acatgtttta atttataagg agatgcttaa
    45361 acatgaaaga atttaaagaa ttggaagatt ttattggaac tcactttatc tatacgtatg
    45421 ataatggttg ggaatatgaa tggtatgcca aaaatgacca tactgttgat taccgtatcc
    45481 atggcggaat ggtcaaaggc cgttgggtaa aagaccaaga agctgatatc gttaaattga
    45541 cggacggcgt attcaaaatc acttggacag agccaacagg gacagacgtg gcattggact
    45601 ttatgccaaa tgaaaagaaa cttcacggaa tgatcttttt ccctaaatgg gttgaagaac
    45661 atccagaaat cactgttact ttccaaaacg aacatatcgc agaaatggaa gcagctcgtg
    45721 aaaaatatga aacttatcca aaattacttg ttcctgaatt tgctactatc acttatatgg
    45781 gtgatgcagg tatagacaat gaagatgtta ttagcgaaga accatacgat ggaatgccag
    45841 atgatatccg tagcggaaaa tatttcgatg aaaattataa acgagtaaaa taaggttgtg
    45901 aaagagaggt tgtgaaatca gcgttctgac ctgagtatta gagaaagaaa cggaaaaata
    45961 gttcctcatg ttttgccgtt ttttgagcta ataccgaggg tcagctgatt gaacaccgtt
    46021 taagaaagag gttgtgacag aagtggtcag cttcaagaaa taagaaggaa tttacggaaa
    46081 ttgcttctca aatttttgta aaatttcggc ttatttccga agaagctact tttggaacac
    46141 cgtgaatcag aggttgtgaa aaagctgttc tgcatcaagt aataagaaca gccaaataaa
    46201 aatagctcct catatttttt gtttggttgg gcttattacc gtagatgcaa gcttttgaac
    46261 accgtttatc aaagattgta acaactgcat attcacacag ttaaattagt gacttcaaaa
    46321 tgaaataatg ctcccagcaa aaaattaggc tgggagcatt atttcgttat tctgttcatc
    46381 aaaaatcagc gttttgattc catttcatca caagttcctc ctcatttact gcttcatctt
    46441 tttgctgtgt ttgaattgta aaaccttgtg aaagatagaa atgaatagct gattgatttt
    46501 tttgatagac tgtcaaagtc agtgttttat tggtttcttt tatttttttc attagttgcg
    46561 taccgattct ttgttgccgg tattcccttt tcacaaagat tcctgcgata aactgatcaa
    46621 tcaccccagc aaaaccaata atttcttgat ctatttctgc taagtaaatc actgcatctg
    46681 gtaaagcttt cctaacagat tctacttgat tctcccaata agttgatgga atgaattcat
    46741 gagaatccaa gttagcatgt agccaaatcg atagaacaga ttccagttct ttggcagaca
    46801 attttgttgt tgtgcggatc attttttctc tccttactta ttcattgatt ttaaaataaa
    46861 aaccattttt ttgaagctct tttacaagtt ttcgcccctc aaaaaagtat atcttctctt
    46921 gttttacttt tctaatacca aaagaaacag caactttagg atcatcagct gaaacgattc
    46981 gataagtccc accaggcaat tgatccagct tgactttccc atgatggtcg ctttgtactg
    47041 aaaacggcac accatttcta gtaagtgttt ttttcttatg tttcaaaatg aatgaactac
    47101 catttaccgg tttccctgac gaatcactaa taataaatga aaaatcaagc acttttttac
    47161 ttaatctata ctgatagatt agtcgtccga taaacactaa cagtatgact acagctatta
    47221 tcgttcctgc aagaataaac cattgccgca tggttctttc ctgatttcct tttccttctt
    47281 ctttttttac cgattcgtta taaggaatcc gatgtcctgt cactagcaaa cgatgcgtgt
    47341 tgatcatata cggcgtacat gtaagcaagg taactaagtc ttctccttct gcaatcgtta
    47401 gaacagacgt gtcatctggc agtaccactt gtatattttt aacttgataa gccaatttct
    47461 ctcccaaaac agtcaaaata aacgtgtctc cgttttctaa acgatctaat tcggtaaata
    47521 accgtcgatt aggtaaaccg ctgtgagcag agatcactga atgcgtcgaa tttccaccaa
    47581 gcgggaaaga ggttccttgc aagacgcctg ctccattttc aaggatccta ccattgagcc
    47641 gatcataaag tgaaatattt aattggatct taggtatatt gatacttccg atcagccatt
    47701 cctctaattg gctggatgtt gtactttcat ttctgttctt ttcattgaaa ggatcagtac
    47761 caggattgaa tccttggtca gttaatcgtt ggtttttctc ctccattttc tttcgctgca
    47821 cttcaatttc agctgcggtc cgtttttgta cttgttcgat acgtttttga tcaataaaac
    47881 tatttaaaga atccacataa aatggatata aagcgatcag taaccctacg acgatacata
    47941 ttaataaaaa caaacgaaac aaaagtcgac gagactgttt ttgcttactc tgcttcacat
    48001 gtgtcctccc ttgcaagttt ttttgttctt tcttttatta tatgggttca ggaaaaaaat
    48061 agaaaatgaa agccgatgag attgtgaaaa agctgtcctg catcaagtaa taagtacagc
    48121 ctcaaaaaat agctactcat attttttgtt gggttgggct tattaccgca gatgcaagct
    48181 tttgaacacc gtttaaggaa gaggttgtga aaactgcgtt atgctttgag taataagagt
    48241 gctagtcaga aaatagcttc tcatattttc taggctagtg agcttattac cgagaagcag
    48301 ctgattgaac accatttaag gaagaggttg tgaaaactgt gttatgtttt gagtaataag
    48361 agcgctagtc agaaaatagc tttttatatt ttctaggcta gtgagcttat taccgagaag
    48421 cagctgattg aacaccgttt aaggaagagg ttgtgaaaac tgtgttatgt tttgagtaat
    48481 aagagcgcta gtcagaaaat agctttttat attttctagg ctagtgagct tattaccgag
    48541 aagcagctga ttgaacaccg tttaaggaag aggttgtgaa aactgcgtta tgttttgagt
    48601 aataagagcg ctagtcagaa aatagctttt catattttct aggctagtga gcttattacc
    48661 gagaagcagc tgattgaaca ccgtttaccg agaggtcatg aaaaagctac tttgtttcga
    48721 gtattaagaa aaattctgtt tcaaaacaag gagatgcaac aaaacttatg tcgcatcctc
    48781 tctccttaga actttcactt atgtttttct tgtaaaattc ataaatattt tcatgaattt
    48841 tctaatttct tgcctacctc tcgcatagga tatatgatga atttatctaa agtttgcaaa
    48901 ggagttcgac aattgaaaaa atatggccca tttttagcaa tttttttcat tgctttggca
    48961 tttctcatgc ctcatttatt cacacataaa ctaattttgg gtgctgatgc cccattccat
    49021 tacaatcggt tctatgatgc cgcgcaacaa attaaatatg gggatttcca ttattttcct
    49081 tccctttatg gattccagca aagtggcagg atcgtgaatg ccgtttacgg cccatttttc
    49141 gcttatttac aaggattgat cgtgttgctt gcaggtagct ggtttcatta tcagattctt
    49201 tcaaatttca ttctttatct tatttcagga ttttctatgc tgattttact gagatatgtc
    49261 aatattcgaa attgggtaag tgtaccagta gccgttttat tcatgagtac gtattctatt
    49321 caatactggt ctatcaacca aggattcact agctggggga cttgttttta tcctttatgt
    49381 atgatcccta ttgctgattt cgtccttcat aaaaagtttc ctgttatcaa agtcgctgtc
    49441 tcagtgggac ttatgacaca gatccattta ctgtcttcga tcatgttagc tttgatgtat
    49501 attccttttt atctttatta cttttttaac cagactgaaa aaggaaaagc actgcttcaa
    49561 ctgattaagt cggtcttgct ttattttgtt ctgacagcaa atatttgggc aggattagct
    49621 attgtttaca gtgggaataa gattcttcct ccctttgtta acagagaaat gagtcaaaag
    49681 gcgatagatc tggatggcag ttattggctc cattacccta aggcctttcc tatattgata
    49741 ttatcaggtt ttttgctttt tattttcttt caacaaaaga acaccttatt ccaaaaaatc
    49801 gtgttgtaca caagtcttgt tttctttttt ctttctacta gcttgattcc atggacgaca
    49861 ttgatcgaaa aaaatgtgcc ttttattagt ttgatccagt ttccatttcg atttttcgtg
    49921 ccatttacgc tattgcttct actttacctt gccctttcac tgaaccagtg gtctgaacaa
    49981 aagtggttcc gtcttttaag tatatttggc attcttattt tttcagcaca gacgatccag
    50041 aacttatacc agcatctgga aaagtgggaa aatgaaacat ttgtcagtcg acatacttat
    50101 ctattcgata cgccggaaga agcaaggaaa acattcttca acaaagatat gtctaccagc
    50161 ttgttcgttt tccaaaaaac cacacctgac tatctgccaa tctataagga gacaaaagac
    50221 aataaatacg accggtataa cgaagaaata ctagaaaacg aaacaacttt tataaaaaca
    50281 catccaggcg gtcttctgac aatcaagtgg aaaaacactg gggacaaaac tgttcatatc
    50341 cctgttatcg tctacgatcg gactgtgcta cagcaaaatg gaaaaacatt gactgattat
    50401 gaagtaacag atatcgggac cccaatcgtg aagcagcaaa aaggaatcaa cgagctcacg
    50461 cttcattatc aaacgccaat ctatttctat ttcattcttt ctcttacttt gattggctgg
    50521 tttaccttgc tatgtttatt tatttatcat agatacaagc atttacgtgc ttaggcaaaa
    50581 gggataaatg catccgatca aaaaactctc caaacggtat aatcgtttgg agagttttat
    50641 tttaatcagt ttttttatgt tatatgttat attttttatt cgttcacatc taattcgatt
    50701 tccaccccgt agtgatcact gatgattgga ctatcgatcc catcaaaaat ccgttgatat
    50761 tttttaacct gcattccttc agggacgaaa ataaaatcta tacgcaattt ttctgtattt
    50821 tgttcccaac catcgatttt cttttccaca gtagctgctt ttccttcttc attcgcgaca
    50881 acatacgaat cttgaagaag aaggcaactt tctcttacag tataataacc ttgagagtct
    50941 actggattat tgaaatcacc taggaaaaaa agtggtacgc gagtttccaa aaagtagttt
    51001 tccaagtttt tccactcaaa ataaaagcca ctttttttat ctgtccacca agaaaagtgg
    51061 cagctcgaga caaaaatgtc ctgatcatct atctttgttt tcccaatcaa gatcttgcgt
    51121 gtctgataat cttctttctt ttgagattgg gagacggtaa ggacctgact ttccaatgtt
    51181 tttttcgcaa gcagtgcatt tccttcttca tatttatcat aaccgatgtg gctcatttcc
    51241 caactccaaa aatactcttt tccgtgttcc tttaaaagct gaactaaacg gtaggcaaaa
    51301 ttatctttac ggataggtac ttgattcttt accggacaga acttcattag cttcgcaggt
    51361 tctacttctt cagattccag taattggttg atttcttgta aagcaatcac ttcataatct
    51421 tctaacagga tcttatctgc taattcttgc aattttttct ctggttctgg ctcaagccag
    51481 ctatgcgtgt tgagtgtcat gaatttcatt ggttttctcc ttgtcttcat ttcgaaacaa
    51541 aagggttgtg acaaaatttt tgtcacaacc tctccagcaa atagtgttct cctgctaact
    51601 cctcagtaat aagctcaatg gaccaggagc tattttttgt ttggctgttt ttatcacttg
    51661 gtgcagggca gctttttcac gacctctcca ctaaacagta ttcaaactgc tgcttctcgg
    51721 taataagctc actagcctag aaaatatgaa aagctatttt ctgactagcg ctcttattac
    51781 tcaatgcata acgcagtttt cacaacctct tccttaaacg gtgttcaaaa gcttgcatct
    51841 gcggtaataa gcccaaccaa acgaaaaata ggaggagcta tttttgtttg gctgttctta
    51901 ttacttgatg caggacagct ttttcacaac ctctcgataa atggtgttca atcagctgac
    51961 cttaggtatt agttcaaaaa acggcaaaat acgaggagct gttttttcgt tttgtttctc
    52021 taatactcag gtcaggacaa tgatttcaca acctctcctt acattttaat ggagccgatc
    52081 actttaccag ctgtcactgt acctaattgc ttgatcacta cttcttctac tttatcgttt
    52141 gtaaatacga tcatgatcgt tggatctttt tcttcatttt ggatcacctc aagatccatc
    52201 tcagctaatt tcgttcctgt tcctacttct tggccttctt tgacagcaat ctcaaaagct
    52261 ggctgattca tttggactgt atcgataccc atatggatca aaacttcgat accatctttc
    52321 gtctccagcc caattgcatg tttgcttggg aaaatacttg tcacttttcc agcgatcggc
    52381 gcaacaactt ctcttgatgt tggaataaca gcaaagccgt cacccatcat tttttgtgag
    52441 aataccggat cttttacttc agataaagga atcacttctc cagaagctgc ggaaactagt
    52501 tcttgttcta tacctaagta ttgcttacta tctgcttgtc tatttcctgt agaaaccatt
    52561 tctggctctg gaatctctgc tcctgattgc agcagatctt cgatatctga tttcaagaca
    52621 tctgcttttg gtccataaac tgcttggact ccattgtctt tgacgatcaa tcccatcgcc
    52681 cctgctcgtt tccaggcttc ttcagtccct actttttctc gatcatttac acttacgcgt
    52741 aaccgagtca tacaagcatc gacatctttg atattttctt ttcctcccag caaatgaatg
    52801 atctggacca cttgctgatc tgctgttcct gttccgctgt tagatacaac ggtttcttca
    52861 ctgtcattgt cgtaatttcc gttacgtccc ggtgtagcaa agttgaattt tttgatcaag
    52921 aaatttgcta agaaatacgt aacaacacca aaagcgatca cacacaacac gaagttgata
    52981 agatctccac ctaaacccgc tttgatagct aacggtgtcc gagtcagcaa ttcgatattc
    53041 ccaaatgaat ggacccgcaa tggcaagata tctgccatcg caaatgcagc cccttggatc
    53101 actgaataaa tgacatataa tggcacagca gcaaacataa acatgaactc caatggttct
    53161 gttacacctg ttagaaagac agccaacgct gctgaaaaat acattgattt atatcttgct
    53221 ttcttatcag gatcgacatt acgatacatc gcaaaagcca ttcccatcaa gatacctgaa
    53281 gaaccaatca tttgtcctac tttgaaacga gccggcgtcc agttatgcaa cacaaactca
    53341 tattgcgaca tatcccctgc acctttaaga ttcactaaat cagtggccca agccaaccat
    53401 aacggatctt gcccaaatac ttgtgtcccc gcctgtgcgc ctgataaaat ctcatacgat
    53461 ccgccaagct gtgtatagtt gataggaatc gtcagcatat gatgcaatcc aaatggcaat
    53521 agtaaacgtt ctaatgttcc aaataaaaac ggtgctagaa tcggtgcgct ttcttgtgat
    53581 tctgcgatcc ataaaccaaa attattgatt ccagcctgta cgtttggcca caccacagca
    53641 agaatcaatg ccacgattgt tgaccataag ataacaacaa acggaacaaa gcgctttcca
    53701 ttgaaaaatg acaaagcatc cggcaatttt ctataattat agtacttatt ataagccatt
    53761 gccccgacaa atccagcgat gatccctacg aatactccca tattcaaagc tggtgcttcc
    53821 aatacacttg taaaaaatcc tttaaccatg atcttcgtac cgaacagagt gtgggtaaat
    53881 gcatcttcat tcgcaagcat gtcacttgtg acaccaaaga tagccccagt gatccgattg
    53941 atcaaaataa atgagatccc agctgcaaat gctccgcctg ctctttcttt tgcccaactt
    54001 cctcctattg ctaaagcaaa taaaatatga agattaccaa taattgccca accaatattt
    54061 tcaattactc cgcctgttgt gacaagcggc gtccaatcag gattgatcaa gggaattgat
    54121 ttccctatac tgatcatcaa cccagctgcc ggcataactg ctaccacaac cattaaagcc
    54181 tttccaaatt tttgccaaaa ctcaaaacta aatatctttt tcattccgtt tagctccttt
    54241 acgcaatcgg tttcgttaaa cgtattatag cgtgcgtttt cttttttgta aatgctttat
    54301 tttttatttt tctgtctata ccattctaga aaacctataa tgcgtgtgtt cccttctata
    54361 attttctcct aaaaatttat ttttttatgc ttttttctat gaaatatttt cttttgtcta
    54421 ttgacttgtg caatcggttg cgtttattat gaacgtataa aacagctcat taggaggaga
    54481 aaaatgaaac agatcaaacg actatttgct attgatccat ggaaaatcag cacgacacaa
    54541 ttagataaaa aaaatcttcg actgcaagaa tctttaacaa gtattggaaa cggttatatg
    54601 gggatgcgcg ggaactttga agaaacttac tcaggggacc accatcaagg tacttatttg
    54661 gcgggtgtct ggtatcccga taagacacgt gtcggctggt ggaaaaatgg ttatccagaa
    54721 tactttggaa aagtgatcaa cgccttgaac tttatcgcga tagatatttc gatcaacgat
    54781 cagctgatcg acttggcttc tttggaacca gaagactttt actgggaatt agatatgaaa
    54841 aatggtgtat tatctcgtac attcacaatc actacttcta ctaataaagt acgcatttcc
    54901 ttcgaacgtt ttctgagcat tgtcaaaaaa gaatcagctt atatctattt aactatagaa
    54961 atgcttgaag gtagcggaac agtcaaactc gtatcaaaat tggatggaaa tgttcaaaac
    55021 gaagacagca actatgatga acatttttgg aatgagattg atcgtggaac tatagaagaa
    55081 atcggctatt tgacgactaa aactattcca aacaactttg ggattgaaca atttactgtt
    55141 actgcagcaa tgcgccattt cgttaatgga aaatcagtca tccctgacta tcacgaagat
    55201 actttagcat tacatggttg cttgtctgca gaattatctc aaggtgagac atggtcagtg
    55261 gaaaaagaaa ttatcgttct tactagtaga gatattccag aagcgcagca agcagaaaaa
    55321 gctgtctcag aattaacgaa ccagacaaac aattatatcc aagcaaaaga agagcaaaca
    55381 gcggcttggg cagaacgatg gaagcttgcc gatgtggtca tcgaaggaga cgaagaagca
    55441 caacaaggta ttcgattcaa tttgttccaa ctattctcta cttactacgg cgaagatgaa
    55501 cgattgaata tcggccccaa aggattcaca ggagaaaaat acggcggagc cacttactgg
    55561 gatacagaag cgtatgctgt ccctctttat cttgctttag ccaaacctga agtaactaag
    55621 aatctgctga aatatcggca taatcaactg ccgcaagcaa tccacaacgc tcagcaacaa
    55681 ggattaaaag gcgcgcttta tcctatggtc accttcgctg gtgtcgaatg ccataacgag
    55741 tgggaaatca cattcgagga gattcaccga aatggcgcga ttgcctatgc aatctacaat
    55801 tatacgaatt acaccggcga tacggaatat ttgaaaaatg aaggactgga agttttagca
    55861 gaaatagcac gcttctgggc agaccgtgtc catttttcaa aacgagcaaa taaatacatg
    55921 atccacggtg tgaccggccc aaacgaatat gagaacaata tcaataacaa ttggtataca
    55981 aatacgatcg cagcttgggt cttgcgttac acgcgagaaa attatttaac ttatcaaaaa
    56041 gaagctacta tcactatcag cgaagaagaa ctagccaaat ggaaagatat cgcagagaac
    56101 atgtacttcc cagttgacga agaattagga atctttgtcc agcatgacac attcttagat
    56161 aaagatctca tgcctgtatc cgatattccc acaagcgatc ttcctctaaa tcagcattgg
    56221 tcttgggaca aaatccttag atcttgcttc atcaagcagg cagacgtact acaggggatt
    56281 tatttcttca atgatacttt tacaaaagaa gaaaaacaaa aaaactttga tttctatgaa
    56341 ccaatgactg tccatgagtc ttccctttcg ccaagtatcc attcgatttt agcagcagaa
    56401 ttaggaatgg aaagcaaagc tgtagaaatg taccagcgaa cagctcgctt ggatttggat
    56461 aattacaata acgacacaga agacggcttg cacatcactt ccatgactgg tagctggttg
    56521 tcgattgtcc aaggatttgc tcaaatgaaa actgcccatg atcagttgag attcgcacca
    56581 tttctgccaa aaacttggaa agcttattct ttccatatca attatcgcgg acgtttattg
    56641 ttcatctcag tcacagatga acaagttact ttcgagttat tagaaggttc tcctctagaa
    56701 atttacgtct atgaacagtc ttattcttta gcggatcgtc tagaaataac gctaagaaag
    56761 gaggaagcaa atgtttaaag gggtattgtt tgatttagac ggtgtcatta ccgatactgc
    56821 tgaattccat tatcgtgctt ggcgaaagct tggtcaggaa atcggcatta cgatcgatcg
    56881 tacattcaac gaacagctaa aaggcgtcag ccgagaagac tcactctctt tgcttttagc
    56941 tcatggcgga aaagaacact ctttttcaaa ggaagaattt gcagaattag cgaaaagaaa
    57001 aaacgattat tatttagaga tgatccagac gatcgaacca aaagatgtct tccccggagt
    57061 cgtgccttta ctagattctt taaaagaaga aaaaatcaag attgctcttg cttctgcaag
    57121 taaaaacggg cctttcctac ttgaaaaaat gggtcttaca ccttactttg atgctattgc
    57181 cgacccggca gaagcaacaa acggaaaacc tgcgccagat atcttcctcc ttgcagcaaa
    57241 agctgtcgga ctaaaagcag aagaatgtat cgggatcgaa gacgcgcaag ccggtatcca
    57301 agccatcctc tcaagtggcg ctcaaccaat tggcgtggga cgtgctgaag accttggaga
    57361 ggagatcccg cttgtccctg atacgacttt tcttacgatc gattacttga aagaaaagtg
    57421 gcatgaccat ggataatcca gaaatctccg tcgtaccagt tccagagact gaaataaaga
    57481 aaatcacatt gaagaacgac tacttagaag tcgttcttct caattacggt gcccgcctgc
    57541 accaaatctt cgcaccagac aaagaaggaa aatcagaaaa tgttttattg tcttacgatc
    57601 gtttcgaaga tgttctaaca gacaaaagct tttttggagc aactgtcgga cctgtagccg
    57661 gaaggatccg ggatgcttct tggggtgagc atagattgga gaaaaactgc ggatcacacc
    57721 atatccacgg tggcacgaaa ggctggtctt tccaattttg ggaagtagaa gttttcaaaa
    57781 ctccatcatc aataggtgtg gtcttttact taaaagatga attttctacc tatccaggac
    57841 caatcactgc tacgatcact taccgtttaa caaaaaacga attagagatg acaacctctg
    57901 ctagtagcct tgttgaaacc atctgcaatc cgaccaacca tgcctacttc aacctttcag
    57961 gaaatggaac aagagacatc tatgaacatc agttaacggt ctttttagat ggtgtgcttg
    58021 aattagatca agaaaaactg cctactggaa attggacaaa aaaagaagaa ctgccaatcg
    58081 actttagaaa atccccaaca ctgcaagaga tccttgcatg ttatccagat ggactagatg
    58141 atgtatttct tttgcatcat cctaggcttt ccaaaacttc cttacaatta ttcgagaagc
    58201 attctggtcg caaaatgact atagcaactt ctaataaaag tatggtatta ttttctacga
    58261 ctggattcga agctgatttc tctgtcaatg gaaaacagat gcattcaaac tacggattag
    58321 caatcgaacc gcaagaaatc cctgatatcg ttcatttccc caaatttgga tcgatcaacc
    58381 tccatcctgg tcaagaacgg atcagtcaga cgatttatcg gttctctgca caataacaag
    58441 agaaaggtgt ttttatcatg gctattaccg taaaagatgt ggcgaaaaaa gccggtgttg
    58501 ccacctcgac cgtctctcgt gtcatcaacg atcatcctag tatttccgag acaacaaaga
    58561 agaaagtccg aaaaattatg gatgaactag gttatgttcc taacatgact gcccgcaatc
    58621 taggaaaacg catttccagt gccatcggcg taatcttacc ccctcttgat tcaaaagagc
    58681 ggatcggtaa cccattttat cttgagatca tggaagcaat caacgaagaa gcgcggaatt
    58741 atgatatgac tacagcaatc gctactgcta aaaattttga attgctgtta gagaacgtcc
    58801 agcgtatgca tttacaaaaa caagtagatg gttttattct agtgtattca gatagtcaag
    58861 atcctgtcat tgattattta taccaaaacc acatcccttt cactttgatc ggacagcctt
    58921 atcatcatga gaacgaaatc gtttatgtag ataatgataa ccagcttcta ggtaaacaag
    58981 caacagaatt tttgatccag aatggacata agaatatcct gtttgtgaca aacacgactc
    59041 atgaaagttt gtatttcgaa cgatatttcg gttatcaaaa agcaatgatg atggctggtc
    59101 ttcctgcgtt atccagtgta gtactagagc atccggaaga ttatctgtcc tttgagaaaa
    59161 cgttgaaaga aaacgaagcc acagcattgg tggtcattga cgatatcttc gcccttcgca
    59221 cgatgcagtt ggcgcaaatg tacggctatg tcattccaga gacactctcc gtcattagct
    59281 tcaacaactc gatcttctca acattaacac acccttacct tacaagtatc gatatcgata
    59341 tctccgaatt aggacgtgtc gctatgcaaa agctgcgcga attgatccat gaagaattga
    59401 gcagtggcgt acgtttggtc atccctcatc gcttgatcaa aagagagaca gtcgttcctt
    59461 tagaaaaccc atactaaata gctgactcac taaatacgct gaccatttat cctatggtca
    59521 gctttttatg taaaagaaga tactacttct aacacattgt ttatctgtaa aaaagagcgt
    59581 gggacaaaag taaagaacac ttttgtctca cgctctaaac acgtataaac ggcggaagca
    59641 gaagcaactc cttcggaaat aagctgaaat tcacaaaaat ttaaaaagca attttcttga
    59701 gtttcttctt atttcttgga gttaaacgct tctgtcccag cctctttttt ccttccttca
    59761 attttttaca cgtcgacgat tcaataaaaa accgcctaat attgtaaaga caggtgcaag
    59821 aatcgagaaa aaggcgaatg gcaagtaatg caacgcgcta actttcatcg ttcccataat
    59881 gaatgtgccg ctgactcccc acggtacgag agaattgacg gtcgcacctg aatccgctag
    59941 tgttcgtgtc aaataggttt ttgataagcc aacgtcatca aaagaacgct tgaatgtttc
    60001 tcctggaaga atgatggaca gatactgttc tccaacgatc agattgattc cgatacagct
    60061 caaggcagta aacccgacta aacgagaagg tcggtccatc ttttctttta attcttgaac
    60121 gatggtatca acgatcagat atttgatcag taacccacct aatgccaatg caagtaaaat
    60181 caaactagca gaacctaaca tactcatgat cccgcctcga gaaagcagtt catctagctt
    60241 tggatcaggt gtatttgcga cataaccatt catcaagatg tctgctgctt ttccgaccgt
    60301 caaacttttc tcatggataa aagaaagaac gagagcagct gtcgatcccg ctagtaatgt
    60361 agggatagcc ggaatctttt tgattgcccc cccaaataaa ataactagtg gaatcaatgt
    60421 ccaaggagag atccaaaagc tcgctttcat gttttggatc atttcattga tactagctag
    60481 atcggctgaa tgactgttat gtcctaatat cgtataaaaa atcaacgaaa ggacgaatgc
    60541 tgggaaatcc gtataacgca taccagcgat atgttcgaat agattaactc ctccaatacc
    60601 cgctgccaag ttggtcgtat ctgataaagg cgagcagtta ttccctaaaa aggcaccgga
    60661 aacgactgcg cctgtagtca ttgcatcatt gaatccaagt aaatgaccga tccccaaaaa
    60721 agcaattcca atcgtcgaaa tcgttgtaaa tgaactgccg acagtcactc cgacaattgc
    60781 acaaacaaga aatgctgtcg gtaaaaagaa acgcacagaa acaagtttaa atccataaac
    60841 cataatcgta gggatggttc cagataaaat ccatgaagca actaagacac caatcaaaag
    60901 aaaaatcagg attggaatga tccctggagt gattcctttg atgattccgt catggatctc
    60961 atcccatgtg aactttttaa atctgccata aaacaacaaa cacatgaaag ctgcaaggat
    61021 cgggatatgt ggcgagagct ggaatccgat gatcagtact cctaggagtg ccaacaacac
    61081 gatcaataca gcgatacttt cttttaaaga aatattttta ttcatcgtcg atctcctatt
    61141 tcaaatgaaa aggccctgcc atgaaaaaac tattcacttg gcaggacctc ctactaaatc
    61201 agcgacaaaa cgtcggctga aagacttatt tttttatttt tcttctaaac ttttttctac
    61261 ctcaatcccc caagaatctt tcttgaagtg attgatgatc aatggaataa cgaacatgac
    61321 cactgtgata gctgctacta ataatgcatc actcaatgtc gctgtaccta atgtagcaaa
    61381 tacgacaacg atgatagaga agatcagcat gcagctgact acccaagcta atccgtttcc
    61441 gtttttacca attcggtaag gacgttcgaa ttctggatgt ttcttacgca ttttaagcaa
    61501 tgaaacagca atcaaaatgt acacgataca ataaataaca gttgtagcat ttgtaagtgt
    61561 caagaataca gcacttacgt catccattac accataaagc aaagcaaata atgaaataac
    61621 gattgattgt gttagaacga catttctaga tacgccgtat ttgttttctt tatggaatcc
    61681 gaattttggt ggaaggaatc cttcacgagc tacttggatg attgtttttg atggtccagt
    61741 aacccaagct gataattgta aaagcacacc aataaatacc atgaagctga agatatttcc
    61801 gatcacttct ggtaagccta ggatttgaca gtcaatcaag ataggctgcg tgatatttgc
    61861 taattccatt tttccatctg gtacgacatt tgctacaagc atcgcattga tgacattcaa
    61921 taaaactaat ccgatcaaag cgataaatac gccttttgta taattttttg ttgcgtcttt
    61981 caaacgaggc atgtaaacag aactcatttc gatcccaaca aaaataaatg tgataccagc
    62041 taagtatttc aatgaatcaa tatgttccaa gtcagggaaa gcttttgacc atgaaaagtc
    62101 acctagatag ccgtttgaga ctagtccgac tttgaaggct gccaaaacac ctaaaacgaa
    62161 catgatgact actgggatat atacccctag ccatacaccg atgttcccgc cgacttttgc
    62221 catatcgaat ttcaagttca agatcgtgat gatccagtag ataactaaaa tacaacctaa
    62281 aacgaaccag tgattattgc caagttctac attaccaaat gtgtttccta acaatggtcc
    62341 taaagtagaa gcgaccatta ccatacctgg gaacatttgc acccataata gccatgcaac
    62401 gacaaatccc catttgctgc ccagtgctgt ttttacccat aactgaggac cgccttcttg
    62461 aggaagcatc gttgaaagct caccggaaac cattgatatc ggaccggcaa aaaacaaaac
    62521 tgcaaaaatt gaataaaaaa tcaatgtcca accgactgcg gctaacgtag gaatactccg
    62581 aaccgttgca cacagtgcca ttgtcagacc gataaaggtc ccaagaccca ttttacttac
    62641 ttcagattta ctcaccgttg acactctcct tgattttttg gataaaataa cgatgtcttt
    62701 gaagtagttt cagcaataaa cgaaacaatt cagcgaatat ttgaggggat tctttgagtc
    62761 tattgcttga agggtttctt caaagacaac ctcaaaacaa agggatcgaa gagcttaggt
    62821 ctccccttgc tcttcagatc caaacatgcg tcagaaacag ttagtattca agctgttttc
    62881 taacatgatt accttgctta ttttgcttcg cttgccaata attggtctgc gtagattttt
    62941 tccaattttt cttgaagagc tgctttgatt ttttcagcat attcttcaaa gtgttcttct
    63001 ttgttcatgt atggtgtcat tacgctggca cgcaatacag ttacttttcc ggcacggttc
    63061 cattcttcat ctgtgaatcc tagttggttc acaaattgta atgggctgtt gccatagtct
    63121 gggatagcaa agtcagtatg tgaagttaag aactcattac cgtaaatgct tcctttcacg
    63181 tatgaagaat aatcatatac gtcatggttc aatttattca ttgcaactaa gtcatcgttg
    63241 cctttttctt tgaatacgta gtctaccata ttgaaatctg gataagttaa tggatgaact
    63301 tcgatttctt tgtctcctac tttaaatgac aagtcattca agaagttgta gaaacgatgt
    63361 gcaccttcga ttgatgcacc cattaattta ccatatcctg tgacattcaa aggtaagaca
    63421 tggtgtgctg cccaaacgct ggctgctgtt gcccctgctt ttgaaccttc caagatataa
    63481 gccccaagta atgctgggat atctgcgcct ttttcaaata catatgtcgc aaagtaagag
    63541 ataacgtctc tcatgcggat atctttgatg acgataccac cggcagaata tggaacatag
    63601 cccattttat gtgggtcaat cgttacagat tctgcttctt cgattgcttt atatgcactg
    63661 tgtacttctt ctaagatata atctttgttt tctgtaaaga cattgtattt gtaatgaaca
    63721 tcttttaaat cttcaaatgg aatgaagttg ttgtcttcgt ctaagaaaat cgcacggccg
    63781 tatccgccat aagcagcatc tacgtgtaag tagaagtaaa taccgtcttt ttctaatacg
    63841 cgacgcaatg caacgatttt atcgataccg tcgattgctc cttcttctgt tgatcctaca
    63901 acaccgacta cacctaggat tggtgttttt tcagctgcta gaccacgaac gattttttca
    63961 agttcgttga tgtccatgcg atagttgtgg tcaactggca ctggaatcac ttggtctaaa
    64021 ccgataccga tgatgtcggc agcttttagc catgaatagt gtttagtttg tggaaccaac
    64081 cattttccta atttttctaa atgttttccg ctacgtgctg agtgagcttt gatttcgtcg
    64141 attttttctg ggacgctgtc taacagattc atgatttctt ctgttgacaa gttcattaat
    64201 tcccaatcgc tttttccagc aactagttca ggtgtcactt ctttcattgc aagcggcaat
    64261 gatttgatgt tgcgggcata ccaaagtcct tctaagtttg ctaaagaacc atcagcaacg
    64321 atgtggcccc agccatcttt atagctcatt aattttgcaa attccattcc tacttcttct
    64381 tccatttggc ttgttgcagg agaagattca taggcaacgt tattaccatt ccatagcata
    64441 gcaaaattgt acgctagtaa agatggcatc aatgtttcag agttcatatg accccagtaa
    64501 cgtccggcat tatgccaagg aacggaatgg gtacgcatcc gcgctgaaat ttctgataga
    64561 acatcctttg ttttattcac agtgtgttca aaactagcac tgcttttttc ttctggcgta
    64621 ataataggca tgtcttgagg catgtagttt tgacgccagc ctaaatgttc atccaccaat
    64681 tcattcaaca aagctttata gatttggccg ttctcagctt tgtccccaat gaatagggca
    64741 tttaggttta gatctttcga caatgattca ctcatagtga aaccctccta aaaaaatatt
    64801 taaattttgt cgagccctac attacacctg ttcttctatt ctgtaaacta agttttggcc
    64861 tgatttttaa tcatatttta atatgtatca attatccgaa ttgttcacac ttttgaaata
    64921 attaaaaaaa acgagggaat ttacaaatgt aaactccctc gcaaagattg tattaacgac
    64981 aaccagcgtt ctattttagg atgggtttca aaattggaat gttgtaatag tatttccctt
    65041 tttcaaggta aaaacttact aaattctaat caattacttt ggccaaaaag taatttttct
    65101 taccttttcg aataaccaca aacttaccat cgaattcatc ggatggattt atgacaaaat
    65161 ttaaatcggt aacgcggtca ccgttaatac ttatagctcc gtttgaaaca tcttcacgtg
    65221 cttgacgttt agatggttca atttttgtgg aaacaagcag ttccacgata ttttcaggtg
    65281 tgcttgaaat ttcaacgttc ggcattttac cgaatccttg cgcgatttct tccgcattta
    65341 gttctttaat atttccagag aacaatgctt gcgttatttt ttgcgcttct ttcaagtctt
    65401 cttcactatg cacaaatctt gtaacttctt ctgccaaacg gcgttgtgct tcacgtttct
    65461 caggttctgt ctctactttt ttcgctaagt cttcgatttc ttcttgagac aagaaagtaa
    65521 agaatttcaa gtatttcact acatcgcgat catcttgatt caaccagaat tggtagaact
    65581 caaatggtga agttttcttc ggatcaagcc aaacggcacc gcctgcagtt tttccgaatt
    65641 tcgttccgtc agccttcagc atcaatggga tcgttaaacc gaacgcttct acttctggtc
    65701 cttctaattt tcggattagg tctaatccag ctgtgatatt tccccattgg tctgctccac
    65761 cgatttgcaa ttgcacatcg tatgttttat gcagatgcaa aaagtcgata gattgcagaa
    65821 tttggtaagt aaactctgta aatgagattc ctacttccaa acggctggca acgatatctt
    65881 ttgctaacat cgtgttgatg ttgaagtttt taccgtagtc tctcaaaaat tcaagtaaag
    65941 agatttttga caaccagtcg tagttgttca caaatgtgat atttgcatct ttaccaaaca
    66001 attttctcat ttgattggaa agtgcgtcca cgttatgttg cacttgttcc atcgtttgca
    66061 acacacgttc tgttttgcgg ccgcttgggt cgccgatcgt tcctgttccg ccgccaatca
    66121 gaatatatgg atgatggcct gccaattgga atcttttcat catcataaat ggaatcaaat
    66181 gtccgatatg catcgaatca ccagttggat caacgccgca ataaagtgaa atgctctttt
    66241 cttcaacgag ctcgcgaagt ctttcttcgt ttgtttgctg attaatggcg tcgcgccaag
    66301 ttaattcatc aataatattc attttactga cctccgtgta gtttttttat gaaagcagga
    66361 acctgctagc atgtgtaatt aattatttct tcaggatagg ataggaaata aaaaaagtcc
    66421 cttggcatat aagccaaggg acgaaatctc gcggtaccac ccaatttgta tgtctagata
    66481 tccaacatac ctctcgaaca tttaacgctg tcgcgtggca atcactgcca aagactcctg
    66541 aaatgtaatt cgctttaaag catgtactgg tttgcagcga ccaccagctt tctgaaacat
    66601 tggctttaac aactacttcg tttcgatctt cgtcttgtta ttattaatat gtgtctatca
    66661 tagagcgcaa ctttttcttt gtcaacagaa aaaggatatt ttttttaaat tcctcatttt
    66721 ttcttttttg aatccgctct aattccgaac attttatttt caattattgt taaaagaaat
    66781 aactttttct gtattttttc tgcaatttat cgagaagtca ctttactttg cctcttattc
    66841 tttcttcgtt acaataagga atgggacaag taaaaaaatg aaaaagcaag ggacgaggta
    66901 tatggaaaaa aagcaaaaaa ttattttagg agccacttct gctttaacta tcgttatttt
    66961 aattctaagt ttgttttttg tcatccgatc atctacaaaa aaagaaccaa tcattcctga
    67021 aaaaaaaata acagaaaaag caaaagaacc gacagatgat aagcaacaac tagttactca
    67081 agcaaaaaat ctggctcaaa gctacgatta tgaccaagcg attgcgttat tggaaaaaga
    67141 tagtaagaac ggatctactg aaacacaaca gcttcttcaa acactaaaaa aggagaaaga
    67201 acaactgatc gactggtctg atcctactca gatctctcat gtctttttcc acagcttgat
    67261 cgtggacccg caaaaagcct tccactctca acaggcacaa ggatacaaag attatatggt
    67321 gactgttgaa gaattcaacc gttcgatcga acaactttat caaaatgatt ttgttttagt
    67381 taatttaaat gatttgatcc aaaaagacga acaaggaaat ctttccttta acgggcttgc
    67441 gctacctgaa ggaaaaaaac cattggttct ttcacaagat gatgtcagct attatgaata
    67501 tatggataat tccggttttc ctagccgatt ggtcgtatca aataacaatg aaatcaaaaa
    67561 tgtctataaa gagaaagata aagaagaaat cggtgacttt gacatggttc ctctgatcga
    67621 tgcctttgtc aagaaacatc ctgatttttc ttatcgaggc gcaaaaggta ccttggccct
    67681 gacaggttac aacggagttt taggttatcg aacatcaaaa tctcaatatg gagacaatga
    67741 aaaaacaaaa aaagagatta aagaagctaa aaaagtggcg gaacagttga agaaagacgg
    67801 ctggagcttt gcttcccata catggggtca cttgaatatg actcaagctt cactagaaga
    67861 tgtaaaaaaa gacaatgaat tgtggcagca agaagttgca cctatcctag gaaaaacaaa
    67921 tattctgatt tatccatttg gtgcagacat cagcgactgg cagccttatt cagcagaaaa
    67981 tcaaaaattt gcttacttga agagtcaagg tttcgatatt ttctgtaatg tcgatgcttc
    68041 tacaccagct tggggacaat taggtacgga tttctatcga aatgctcgga tcaatatcga
    68101 tggcattcgt tttcaatctg atttagacgg aaaaaatcct attcttgatc cattcatcaa
    68161 tgtccatgat gtctatgaca aagaagcacg cgaatctgct tgattttacg tcatttatga
    68221 accaaaaaag ccgaactgac attttatcgt cagtttcggc ttttttatgc tttatttctt
    68281 cttcggtgcg attcccttga tcaggacttc tgtccattct gctgtatatg gaagcagttt
    68341 tgcgtatatc tccggcttag aaattccttc taccattgct tgaaaataga ggatcaagaa
    68401 ctcatccgaa tatttcaaat ccacttttcc ttctttccgc cctcgatgga aaagatcaag
    68461 catgatactg aaactttctt gcgtatacgc atccatcgcg tcagaaaatt cttttttttc
    68521 tttttttgta tagtatgtca tcaaatcgat ataaaattgt ttgttcactc gattgagata
    68581 acggattttg ttttcactca tggcaatcag tgtttcttca aatggaagat tttccgcaat
    68641 caacgctttc gcttcttgcc ctaaatcatt cataaatgca aaaaatactt cataaatcag
    68701 cccttccttg ctgccaaaat atttaaaaat cgtcgcttta cttacctgag cgttctcagc
    68761 taccttatcc attgtcactc cagcggttcc tatgtctgtg ttcatcaagt caaaagctgc
    68821 ttgtaaaatc tctttctttt tctcttgtgc tctcttttca aaaccgttca tttgtagagc
    68881 ctcccgtaat tttaaacttt tataatatat atagttcaaa tttattctga ggtttattat
    68941 aactatctat tgagaaagta tcaagctagc aattccttgt ttatgaattc attgacagaa
    69001 atttataaaa agaatattat gtactaaaat aa
//
LOCUS       DAZABD010000007        68627 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000007 DAZABD010000000
VERSION     DAZABD010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 68627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68627
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.007"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(170..1900)
                     /gene="spxB"
                     /locus_tag="ACHALG_000485"
     CDS             complement(170..1900)
                     /gene="spxB"
                     /locus_tag="ACHALG_000485"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047112 - pyruvate oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HFN2585925.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            2065..2883
                     /locus_tag="ACHALG_000486"
     CDS             2065..2883
                     /locus_tag="ACHALG_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HFN2585926.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(2986..3183)
                     /locus_tag="ACHALG_000487"
     CDS             complement(2986..3183)
                     /locus_tag="ACHALG_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2585927.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            3352..3930
                     /locus_tag="ACHALG_000488"
     CDS             3352..3930
                     /locus_tag="ACHALG_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFN2585928.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            4025..4162
                     /locus_tag="ACHALG_000489"
     CDS             4025..4162
                     /locus_tag="ACHALG_000489"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2585929.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
     gene            complement(4975..5478)
                     /locus_tag="ACHALG_000490"
     CDS             complement(4975..5478)
                     /locus_tag="ACHALG_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFN2585930.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            5837..6862
                     /locus_tag="ACHALG_000491"
     CDS             5837..6862
                     /locus_tag="ACHALG_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFN2585931.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            7185..8063
                     /locus_tag="ACHALG_000492"
     CDS             7185..8063
                     /locus_tag="ACHALG_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HFN2585932.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            8539..8655
                     /locus_tag="ACHALG_000493"
     CDS             8539..8655
                     /locus_tag="ACHALG_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFN2585933.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            9583..9921
                     /locus_tag="ACHALG_000494"
     CDS             9583..9921
                     /locus_tag="ACHALG_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2585934.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            10015..10245
                     /locus_tag="ACHALG_000495"
     CDS             10015..10245
                     /locus_tag="ACHALG_000495"
                     /inference="COORDINATES: protein motif:HMM:NF042636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6568 family protein"
                     /protein_id="HFN2585935.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            10645..11016
                     /locus_tag="ACHALG_000496"
     CDS             10645..11016
                     /locus_tag="ACHALG_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HFN2585936.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(11290..11982)
                     /locus_tag="ACHALG_000497"
     CDS             complement(11290..11982)
                     /locus_tag="ACHALG_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFN2585937.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(11985..12770)
                     /locus_tag="ACHALG_000498"
     CDS             complement(11985..12770)
                     /locus_tag="ACHALG_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFN2585938.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            13007..14407
                     /gene="lacG"
                     /locus_tag="ACHALG_000499"
     CDS             13007..14407
                     /gene="lacG"
                     /locus_tag="ACHALG_000499"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFN2585939.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            14432..14749
                     /locus_tag="ACHALG_000500"
     CDS             14432..14749
                     /locus_tag="ACHALG_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFN2585940.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            14762..16435
                     /locus_tag="ACHALG_000501"
     CDS             14762..16435
                     /locus_tag="ACHALG_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HFN2585941.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            16449..18080
                     /locus_tag="ACHALG_000502"
     CDS             16449..18080
                     /locus_tag="ACHALG_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-beta-1,4-galactanase"
                     /protein_id="HFN2585942.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(18571..20136)
                     /gene="guaA"
                     /locus_tag="ACHALG_000503"
     CDS             complement(18571..20136)
                     /gene="guaA"
                     /locus_tag="ACHALG_000503"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFN2585943.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(20195..21118)
                     /gene="coaA"
                     /locus_tag="ACHALG_000504"
     CDS             complement(20195..21118)
                     /gene="coaA"
                     /locus_tag="ACHALG_000504"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFN2585944.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            21314..21919
                     /locus_tag="ACHALG_000505"
     CDS             21314..21919
                     /locus_tag="ACHALG_000505"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFN2585945.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            22227..23243
                     /locus_tag="ACHALG_000506"
     CDS             22227..23243
                     /locus_tag="ACHALG_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN2585946.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            23384..24685
                     /locus_tag="ACHALG_000507"
     CDS             23384..24685
                     /locus_tag="ACHALG_000507"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HFN2585947.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            24731..25393
                     /gene="deoC"
                     /locus_tag="ACHALG_000508"
     CDS             24731..25393
                     /gene="deoC"
                     /locus_tag="ACHALG_000508"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFN2585948.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            25414..25812
                     /locus_tag="ACHALG_000509"
     CDS             25414..25812
                     /locus_tag="ACHALG_000509"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFN2585949.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            25878..26960
                     /locus_tag="ACHALG_000510"
     CDS             25878..26960
                     /locus_tag="ACHALG_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFN2585950.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            27172..28734
                     /locus_tag="ACHALG_000511"
     CDS             27172..28734
                     /locus_tag="ACHALG_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN2585951.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            28727..29860
                     /locus_tag="ACHALG_000512"
     CDS             28727..29860
                     /locus_tag="ACHALG_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN2585952.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            29860..30816
                     /locus_tag="ACHALG_000513"
     CDS             29860..30816
                     /locus_tag="ACHALG_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN2585953.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            30915..32081
                     /gene="deoB"
                     /locus_tag="ACHALG_000514"
     CDS             30915..32081
                     /gene="deoB"
                     /locus_tag="ACHALG_000514"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFN2585954.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            32105..32923
                     /locus_tag="ACHALG_000515"
     CDS             32105..32923
                     /locus_tag="ACHALG_000515"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFN2585955.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            32940..33647
                     /gene="deoD"
                     /locus_tag="ACHALG_000516"
     CDS             32940..33647
                     /gene="deoD"
                     /locus_tag="ACHALG_000516"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFN2585956.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(33945..34631)
                     /gene="gpmA"
                     /locus_tag="ACHALG_000517"
     CDS             complement(33945..34631)
                     /gene="gpmA"
                     /locus_tag="ACHALG_000517"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFN2585957.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(34803..35384)
                     /locus_tag="ACHALG_000518"
     CDS             complement(34803..35384)
                     /locus_tag="ACHALG_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFN2585958.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(35517..36200)
                     /gene="rpiA"
                     /locus_tag="ACHALG_000519"
     CDS             complement(35517..36200)
                     /gene="rpiA"
                     /locus_tag="ACHALG_000519"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFN2585959.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            36519..36932
                     /gene="rpsL"
                     /locus_tag="ACHALG_000520"
     CDS             36519..36932
                     /gene="rpsL"
                     /locus_tag="ACHALG_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFN2585960.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            37016..37486
                     /gene="rpsG"
                     /locus_tag="ACHALG_000521"
     CDS             37016..37486
                     /gene="rpsG"
                     /locus_tag="ACHALG_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFN2585961.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            37580..39667
                     /gene="fusA"
                     /locus_tag="ACHALG_000522"
     CDS             37580..39667
                     /gene="fusA"
                     /locus_tag="ACHALG_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFN2585962.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            39839..41026
                     /gene="tuf"
                     /locus_tag="ACHALG_000523"
     CDS             39839..41026
                     /gene="tuf"
                     /locus_tag="ACHALG_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFN2585963.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            41432..41740
                     /gene="rpsJ"
                     /locus_tag="ACHALG_000524"
     CDS             41432..41740
                     /gene="rpsJ"
                     /locus_tag="ACHALG_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFN2585964.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            41771..42400
                     /gene="rplC"
                     /locus_tag="ACHALG_000525"
     CDS             41771..42400
                     /gene="rplC"
                     /locus_tag="ACHALG_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFN2585965.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            42428..43051
                     /gene="rplD"
                     /locus_tag="ACHALG_000526"
     CDS             42428..43051
                     /gene="rplD"
                     /locus_tag="ACHALG_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFN2585966.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            43051..43341
                     /gene="rplW"
                     /locus_tag="ACHALG_000527"
     CDS             43051..43341
                     /gene="rplW"
                     /locus_tag="ACHALG_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFN2585967.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            43380..44213
                     /gene="rplB"
                     /locus_tag="ACHALG_000528"
     CDS             43380..44213
                     /gene="rplB"
                     /locus_tag="ACHALG_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFN2585968.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTTTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            44256..44534
                     /gene="rpsS"
                     /locus_tag="ACHALG_000529"
     CDS             44256..44534
                     /gene="rpsS"
                     /locus_tag="ACHALG_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFN2585969.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            44557..44904
                     /gene="rplV"
                     /locus_tag="ACHALG_000530"
     CDS             44557..44904
                     /gene="rplV"
                     /locus_tag="ACHALG_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFN2585970.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            44918..45574
                     /gene="rpsC"
                     /locus_tag="ACHALG_000531"
     CDS             44918..45574
                     /gene="rpsC"
                     /locus_tag="ACHALG_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFN2585971.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            45577..46011
                     /gene="rplP"
                     /locus_tag="ACHALG_000532"
     CDS             45577..46011
                     /gene="rplP"
                     /locus_tag="ACHALG_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011375443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFN2585972.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            46001..46189
                     /gene="rpmC"
                     /locus_tag="ACHALG_000533"
     CDS             46001..46189
                     /gene="rpmC"
                     /locus_tag="ACHALG_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFN2585973.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            46214..46480
                     /gene="rpsQ"
                     /locus_tag="ACHALG_000534"
     CDS             46214..46480
                     /gene="rpsQ"
                     /locus_tag="ACHALG_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFN2585974.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            46538..46906
                     /gene="rplN"
                     /locus_tag="ACHALG_000535"
     CDS             46538..46906
                     /gene="rplN"
                     /locus_tag="ACHALG_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFN2585975.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            46943..47251
                     /gene="rplX"
                     /locus_tag="ACHALG_000536"
     CDS             46943..47251
                     /gene="rplX"
                     /locus_tag="ACHALG_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFN2585976.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            47279..47818
                     /gene="rplE"
                     /locus_tag="ACHALG_000537"
     CDS             47279..47818
                     /gene="rplE"
                     /locus_tag="ACHALG_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFN2585977.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            47837..48022
                     /locus_tag="ACHALG_000538"
     CDS             47837..48022
                     /locus_tag="ACHALG_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HFN2585978.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            48058..48456
                     /gene="rpsH"
                     /locus_tag="ACHALG_000539"
     CDS             48058..48456
                     /gene="rpsH"
                     /locus_tag="ACHALG_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFN2585979.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            48488..49024
                     /gene="rplF"
                     /locus_tag="ACHALG_000540"
     CDS             48488..49024
                     /gene="rplF"
                     /locus_tag="ACHALG_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFN2585980.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            49185..49541
                     /gene="rplR"
                     /locus_tag="ACHALG_000541"
     CDS             49185..49541
                     /gene="rplR"
                     /locus_tag="ACHALG_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFN2585981.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            49562..50062
                     /gene="rpsE"
                     /locus_tag="ACHALG_000542"
     CDS             49562..50062
                     /gene="rpsE"
                     /locus_tag="ACHALG_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFN2585982.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            50077..50256
                     /gene="rpmD"
                     /locus_tag="ACHALG_000543"
     CDS             50077..50256
                     /gene="rpmD"
                     /locus_tag="ACHALG_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFN2585983.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            50300..50740
                     /gene="rplO"
                     /locus_tag="ACHALG_000544"
     CDS             50300..50740
                     /gene="rplO"
                     /locus_tag="ACHALG_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFN2585984.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            50740..52035
                     /gene="secY"
                     /locus_tag="ACHALG_000545"
     CDS             50740..52035
                     /gene="secY"
                     /locus_tag="ACHALG_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFN2585985.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            52097..52744
                     /locus_tag="ACHALG_000546"
     CDS             52097..52744
                     /locus_tag="ACHALG_000546"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFN2585986.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            52935..53153
                     /gene="infA"
                     /locus_tag="ACHALG_000547"
     CDS             52935..53153
                     /gene="infA"
                     /locus_tag="ACHALG_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFN2585987.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            53186..53302
                     /gene="rpmJ"
                     /locus_tag="ACHALG_000548"
     CDS             53186..53302
                     /gene="rpmJ"
                     /locus_tag="ACHALG_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFN2585988.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            53320..53685
                     /gene="rpsM"
                     /locus_tag="ACHALG_000549"
     CDS             53320..53685
                     /gene="rpsM"
                     /locus_tag="ACHALG_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFN2585989.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            53713..54102
                     /gene="rpsK"
                     /locus_tag="ACHALG_000550"
     CDS             53713..54102
                     /gene="rpsK"
                     /locus_tag="ACHALG_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFN2585990.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            54182..55120
                     /locus_tag="ACHALG_000551"
     CDS             54182..55120
                     /locus_tag="ACHALG_000551"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFN2585991.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            55148..55528
                     /gene="rplQ"
                     /locus_tag="ACHALG_000552"
     CDS             55148..55528
                     /gene="rplQ"
                     /locus_tag="ACHALG_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFN2585992.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            55800..56639
                     /locus_tag="ACHALG_000553"
     CDS             55800..56639
                     /locus_tag="ACHALG_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFN2585993.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            56615..57484
                     /locus_tag="ACHALG_000554"
     CDS             56615..57484
                     /locus_tag="ACHALG_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFN2585994.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            57474..58271
                     /locus_tag="ACHALG_000555"
     CDS             57474..58271
                     /locus_tag="ACHALG_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFN2585995.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            58474..59220
                     /gene="truA"
                     /locus_tag="ACHALG_000556"
     CDS             58474..59220
                     /gene="truA"
                     /locus_tag="ACHALG_000556"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFN2585996.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(59553..62222)
                     /locus_tag="ACHALG_000557"
     CDS             complement(59553..62222)
                     /locus_tag="ACHALG_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HFN2585997.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(62460..62648)
                     /gene="rpmB"
                     /locus_tag="ACHALG_000558"
     CDS             complement(62460..62648)
                     /gene="rpmB"
                     /locus_tag="ACHALG_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFN2585998.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            62918..63280
                     /locus_tag="ACHALG_000559"
     CDS             62918..63280
                     /locus_tag="ACHALG_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFN2585999.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            63332..65014
                     /locus_tag="ACHALG_000560"
     CDS             63332..65014
                     /locus_tag="ACHALG_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HFN2586000.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            65131..67167
                     /gene="recG"
                     /locus_tag="ACHALG_000561"
     CDS             65131..67167
                     /gene="recG"
                     /locus_tag="ACHALG_000561"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFN2586001.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            67216..68217
                     /gene="plsX"
                     /locus_tag="ACHALG_000562"
     CDS             67216..68217
                     /gene="plsX"
                     /locus_tag="ACHALG_000562"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFN2586002.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            68339..68581
                     /gene="acpP"
                     /locus_tag="ACHALG_000563"
     CDS             68339..68581
                     /gene="acpP"
                     /locus_tag="ACHALG_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFN2586003.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
BASE COUNT        21929 a        12299 c        14709 g        19690 t
ORIGIN      
        1 aaaaaagccg gcttccgatt acagttttac tgttgtaatt ggaagccggc ttttttatca
       61 tggacaaata tgcacgaggt gatttttagg tgtcgagaca aatttatgca aaaatgtgag
      121 aagctatata cacaaatttt atcttaatac tcgaagcata gcgcagttgt cacaacctct
      181 tttcatataa ttcattcaat actggcaaat catggacttc atatttttct ttgaatgctt
      241 ggatttcttc ttcagaatat tttcttggat ctaaatgcag atcttctact ggcaatgggc
      301 gcttgttatt gatcttcaca tcgattacta caggacgatc tgattttgca gctgcatcaa
      361 aggcaaatcg aagttgcgag tattctgtga ctgtaaagct atttgccccg agcgcttctc
      421 caactttacc gaaatctgca tcttctaaaa atacaccaaa tttcttttgt tccgtatctt
      481 cttgttctgc ttcgatgaaa ccaaaagaat cattcgaaaa gacaacatta ataattggaa
      541 gctgatattt gacttgtgta atgatatctt gcatgaccat agcaaacccg ccatcaccac
      601 ttaaggtaaa tacttgtttt tctgggtagc ttagttgtgc ggcaattcct cctggcacac
      661 cattacccat agttgcgaac catccagatg tcgtatgttt ttgtttaccg ttcatattca
      721 ataaacgaat agaatggata gtcgtgttac cgacatctgt gacgaaaatc gcatcgtctt
      781 cagcaatacg attgatttct ttatagacag cctctggacg aatcggttct tcctctcgat
      841 cttcaaaaga tttcaaccaa tttacccatt tttctttatt ttcttgattt gctaggtacc
      901 agctatcagc agggcgagca tcccctaact cgacaagtct ttgcaaagct gtgtttgcat
      961 caccgaggat cgacaaagat gtttcatggc gtcgtccaaa ttttgacgca tcgatatcga
     1021 tttggataaa ttcagcagtt ggattaaaaa atgcacgtcc aaatgggaag tcgctaccga
     1081 caaataagat cagatcggct tctgccaatg cttcatttcc gggtttagta gcgacacgtc
     1141 cagcaaaacc aagaaagttt tcataatcat caggaataat tccttttgct agaacggaag
     1201 cgacaacagg aactgaaaaa tgttctgaga attctttgat ttgcggaaat ccgtttcgcg
     1261 ttccttgacc aacataaaga actggttttt ttgctttttc aagataaggc aaagcagcta
     1321 gcaaatcttt ttcttctggt aatatcacac ttgttttgtg agtgtgagcg gtggagaaag
     1381 gttgttcgtc aatttcttca aaaccaagat caacagggat agtcacgata gccacgcctt
     1441 tatgctcata ggcggcttta atggcttcat ctactacatg cggaagactt tgtggtgtca
     1501 tgactgtacg attgtatacg ctaacatctg cgaaaatagg attttcattt agttcctgga
     1561 aagagttata gttcatggat gtagaagcaa cttgtcctaa gaacgctact agaggcacat
     1621 ggtccatctg tgcatcatac aaaccattga tcaaatgtgt cgcaccaggt cctgcagagc
     1681 cgaagacagc gccgactttc cccgttaatt ttgcatctgc agcggctgcg agtgcgccga
     1741 cttcttcatg acgaacttga atgtagttga tttcggtttt ttcttgatat agcgcatcca
     1801 taattgaatt gaaagatcct ccgggaatgc cgtaaatatg gtcgataccc cagctttcaa
     1861 gaacttttac cattgcgatt cctgcattga tttttgacat ggtgtttcac tcctttagca
     1921 atgatgatac ataaattttt tgtatctaga gtatagaact ttttatcgaa aatcccaact
     1981 aattcgtctt ttcacaaaaa tgtagtcatg tttttgggaa tatgctagag tagtacatag
     2041 atagattcaa gagaggggaa tggcatggtt gaatggctat tgagacttga accttggcag
     2101 caagcacttg ctgggacagc ttttacttat tttatgacag ctcttggtgc cgggttggtt
     2161 tttttcttta aagaaatcaa aaaagaagtg ctaaacttga tgttaggatt tgcttctggt
     2221 gtcatgattg ctgcaagttt ttggtctttg ttagatccgg ctattacaaa agcagaagaa
     2281 aatggagata ttgcttggct agttgtaagt attgggtttg gtttaggcgg tgtgttttta
     2341 tatatggcag ataaaacact gccccatatg cattttggac cacaacatga agcagaagga
     2401 ttgcctaccc atttaaaacg gacgatcttg cttgtttttt ctattacatt gcataacatt
     2461 cctgaaggtt tagccgtggg ggtagcattc ggtgcagcag caactgcgga taatccgaca
     2521 gccgctatat tagccgctgt ttcggtggcg ctaggtattg gtatccaaaa cttcccagaa
     2581 ggagcagctg tatcaatccc gttgcgacaa gaaggtctga gtcgtaaaaa ggcctttgtt
     2641 tacggacaag cttctggaat cgttgaacca attgctggag tgattggagc gctactagta
     2701 acgaaagtgg agctgctgct tccttatgca cttgcttttg ccgcaggagc aatgatttat
     2761 gtcgtggtgg aagaattgat ccctgaagca cagcagacat tgtccagcaa acgtcatttt
     2821 gcagtttttg gcgtcatgag tggatttatc attatgatga ttttggatgt ggcattagga
     2881 taaataaaaa aaatatatat gatatatcaa atagggccgc gaacttatat cgcgacccta
     2941 tttttttagt aagttatatc ataatgaagt gaatggtatc atacattaat aatagttttt
     3001 tctatgtgta tttcctttga tccagcgaac aagccagatg ataagtgcga ttgggattag
     3061 aactttgacc gcaaaccata aaagcccgct taggacactg acaaaaagac tgatgacgaa
     3121 gctagctgca attagcacta aaagaaccat gaagatattt aagaacatat ttttttgacg
     3181 catacaaaac cctcctcttg ttcattcttt actatagaaa aagtataaca gggattgttt
     3241 aaaaacacct ccaactgaag actgattttt acttcatcta cttaaatgat caaacaattg
     3301 ctttttctct ggtttatcat ctaaaataga gcggaaaagg aggagaatat catgcctaaa
     3361 aatcgtcttg aagcatttac agatgctgta atcgcgatta tcatgactat tttagtttta
     3421 gaacttcatg aacctaacgg agacacgtgg gaggcgctcg ctgcattagg ttaccgattt
     3481 tttatctata ttgtcagttt tatcatttta gcgatttatt ggaacaatca tcatcattta
     3541 tttcaagttg tacataagat aaatggacgg gtcttatggg cgaacagttt ctttgttttt
     3601 gcgctttcgc tttttccgtt tgcaacttca tggatcagtg agtacatcaa tagcctggct
     3661 ccagaaatga cttatggttt tgttaccttg accgctaata ttgcgtatta tatcttggca
     3721 aaagaacttg tcaaagcaaa taaaggccac gagaaagcat tgaaggcgat gttgaataat
     3781 tacaagaaat cgtatctatc gattggtttg aatgtgttag gaattgtctt agggtattta
     3841 gtagcgccag tagccgtgct gatcgcaaac atcgtgattc tagctgcttg gatcatccca
     3901 agtcgtacca tcgaatcaca atatcggtaa aatgatccgt gtttaaaatt taagagaaaa
     3961 atatagaaaa aataaataaa cgatcaaata tccgagtgat actagtattc aaagagggat
     4021 gcagatggat caaaaaagaa ttcatgtagg actgattact agttttgtca cgatgcttat
     4081 ttatttggat caaggtcatt tgacttggac agtagttggt gtcgtaggat ttttgacatt
     4141 attcacagca ataaaaaaat aaacatcttt atgtaatctg atgttctatc tatttaagaa
     4201 aagcatgttt acagcagtat gattctgttg taaacgtgtt tttatttgtg gaaaatttaa
     4261 tttttagaaa agtgtatcga aaaatagata gttagttttt ccacagtgga taacttcgta
     4321 agagataaaa ataaaaaatt agtcaaagaa gggcgtatag atttcttttg ataggagata
     4381 tacaacagaa atgaataaat attaacgtgt tttcaaacag tataaaacgg tgtggttttt
     4441 ggaataaaaa aataacatat ctcattttac acttttgtta ttttatactt aaaaaataac
     4501 gacacagaaa ataccgtgtc tcagatttct ctactacttc taaaaactac tgtttttttc
     4561 ctttgaaatg ccttcaaaaa gtaggatcag gcagaatata ctggcagaat caaggagaag
     4621 ggagcctaaa taaggagaaa aaccatagaa aatataaagt aattacttat aaaacaagct
     4681 ttttatagaa aaataatata cctgagtagg gtataaatta agacggaatt cctttctaga
     4741 ttgagatatt gactaggaag aatgttaaga ttttaagcct ttaagctaaa aaaaaaccct
     4801 ttttgttaaa taaggggaat atagcctgtc agtttcaaac tgcaagtcgt cctgctgcaa
     4861 taatatagta gattagagat gaaaatagat ttttttaaac caatgactga ataaaaagac
     4921 cgtcatagat acacattgga atgtgtctat tacggtcttt ttttacgaac gattttattg
     4981 aagacggaca gttcgggtca aaaataaaaa agctacgatc agaaataaga ataaaatgta
     5041 tcccaaaata atggctagac ggtctaactt atttgtcgat agatgtttgt ttagaagttg
     5101 ataggcccat aaacctatca gtcctcctgt tgtattagtt atcaaatcag tgatgtccgc
     5161 tacgccaata ccgaatagga attgaatcaa ttctgccaag aagcttacaa ggaaaataat
     5221 ccctaatttt ttagaaaaag aaagtctctt gaaattgaca ttcaaaagca ccccaaaagg
     5281 aaaaaagaca atcaagttat aagtggtctc acgtaatacc gtcgtatttt caaaagaaaa
     5341 agtagaaaaa gggacgagat tgacattgct gtaagaataa gtgagaaatt gtggaagatt
     5401 aaatgatagc ttgaataaaa tcaaccataa tagaaagaat aaataaatcc ctaataaaaa
     5461 ttttgataca taccgcatag acatctcctc catttaaaaa gtataccatc gaaaaccgct
     5521 gaaaaatcat aaaaaagtat gaagtttaag gaccaacgta gttatcgtac gaaaattaga
     5581 agcttttttt tgttgggaat ccggtttctt taacaaagct aagatagcga gttgcttgga
     5641 aaagttgcta tcttttttct gtcaaaacga ctcggaatgt tcgttttttg ttctaatttc
     5701 tccaagttgt ttactttttg atactaagtt tatgaaaaat caagaaatag ttgttatact
     5761 aggtaatgct gacaaacggg attggcattt catcttggtt cagctttttt tccaattact
     5821 aggaaggagg gatagattgt tcaagactta ttttcgtttc aataaaatac aagataatcc
     5881 ttttcgtatc atgaatctga tgctacttat ctttattgta ttatggttag gttacataaa
     5941 tcatgatttt ttgatttcaa gtttttcatc attagggatc tttacttttt tctattatca
     6001 aaacataccg atgaaatatt tgctgaaacg tatgtttctg atcggttgcg gactgttggt
     6061 tgctttcgtt ttggggatct tatcgactta tgttttatgg cttgaaccat ttgccgtagc
     6121 tttggttgct ttttccagca gattcgtgtt gcgattattc catatttcta aaccaggtgg
     6181 cttgtttttt gcgatgcttt ctgccatggg aacgagcatg cagctgccaa tcgctcaatt
     6241 accgatcgtc tcactttatt tttttatggg tgtcgttttt gccctgattg cggcagtgat
     6301 tacaaaatta ctggattcac ggcctgagca gacaattgaa aaagcaactt taaaagaaag
     6361 atttcacgaa gagcctttag tgatcattga ttctgttttc tacagcgctg ccttatttct
     6421 ttccgtttat gtcagtcacg gcttgaatct gcataatcct tattggttga ccttgtcatg
     6481 tgcatcgatt ttgctagcag aaaacttgga tgcgatgaag catcgacaag tccaatatct
     6541 gattggatcg atgggtgggt tatgtgtgtc ggcattcttg tcatttgtgc cgtttacgca
     6601 gttgcagacc atttttctga tcacttttct atatggaatc gcacaatttc tagttgctag
     6661 aaattatgca gttgctaata tttttcttaa tccgatggcg ttgatgctat ctacacttat
     6721 tcgtggagca tacttgattt ctttgatcga atatcgtttt ttagggatcg tcataggaag
     6781 tttcatcggt ttaggtgttg cgtgggtaat gacggttggt ttgcaacatt atttaacggt
     6841 tgttcgtcaa aaattagaat agaaggacaa atatatgtgc caaaagaggt tatgacaaga
     6901 tttttgtcac aacctctaat tcaaggtgtt caaaagccag attctcagca ttaagtcaaa
     6961 ttcacacaaa aatgtggaaa ggaactttca aaaaaatttt atatttattc tatcaaaaaa
     7021 ttttgtttat gatggtaact gaataaactt tcctgagatg gtcaatgctt tccttgaaag
     7081 tcttacagat attctttctt catagaaagt ttaagtttta tagttagtgt acaataaaag
     7141 tatgtttata ccatatggtg tccggataat cagtcattta aacaatgagg ttcaaagata
     7201 tgaaaagggg aaatttgatg aagaaaataa gcaatgaaat ccaaattctg gcagaaactt
     7261 taactcttcc caaagtcgtt atagagcaaa tgattgtaca agcaaaaaac atccttgatg
     7321 aggtgtttga gcaatatagt catgaactaa tggatcctaa taaagcaaag gcgactgcaa
     7381 agaaaataga caaagtctat gaagaggaac cttttgcagc attagctatt tatctatatg
     7441 cagcttctca aagttggaaa aaaatttatc aaccattgaa gattccaaga actatctatt
     7501 tggcaacaat gaatgctttt actagattta ttcatgaaca tttccaagta acaggaagct
     7561 atcgctttga ccgtggtttt tggatttggc attacactag tggcttgatc tttcggattg
     7621 gtgaattgga gtacgaaatg acatattttc cgaaagggta cacggatcta agattagaag
     7681 gaaagcagtg cctttctctt catattcctt ccgatgcaga tttatctcag aataagataa
     7741 gagatagtta cgaaagtgcc attcgttttt ttgaacttta ttttcctgat taccattacc
     7801 aagtgatgta cacagatact tggttactat cacccaatct tactaaatgg ttgaaaaaag
     7861 agtcaaaaat ctgtcttttt gcagccgatt atcgattatt atcagttgat gaacaggacg
     7921 atagtggagt cccttggatc tttggacgag tagatgccca aatacatgat tatccagaaa
     7981 gtacgagttt acagcgacaa gcaaaagagc aattgcttgc aggcgagcac attggctctg
     8041 gcttaggtat tcttacttca taagtttagt acttcgaaat agtttaaaag agcgtgagac
     8101 aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg aagcagaagc
     8161 aactccttcg gaaataagct gaaattcaca aaaatttgaa aagcaatttt cgtgaatttc
     8221 ttcttatttc ttggagttaa acgcttctgt cccagcctct tttttagtgt tctatcgaac
     8281 acacagtacg ttcaataggt aaactagcta taacaataga tattaaagtt aacggacaag
     8341 agggcctttt ttaggtagct aaaacgtaat aatgaaaatt tatttccaga gataggagtc
     8401 aatcagctag tctgatcgac tgttttaaca agttgacttt cattttaact tttgatacca
     8461 tttaaaacac aaatcgtaat tgtttcgatt tgtgtttttt agcatgacta caatcaaaga
     8521 agaaagtggg tcgtccatat ggaaaaaaca gatatctcaa gtgcataccg ccgcttgaaa
     8581 agcccaaata tcaaaacacg aaaacgtgcg ctgaagatca tcaaagatgt gaaacggaat
     8641 agtgggaaaa ggtaagaaga aggaaactta attaaaaaaa tagccgaaaa acgtttttcg
     8701 gctattttca ataatcggtt tttgtgtaag gtttccgaaa gaactgctgt tgattttcca
     8761 tttttaacat atgataaatt ggtagaatct ttcttttcaa attgatacca ttccctaatt
     8821 ccagttatat agttaactac agaaccacaa agcacgtacg tttataaaag tattatctgt
     8881 agttattttt caattccctt tcaacctctt attttgacca gtagtattga tcactttacc
     8941 ttttaacacc tttgttcgtt tttgaaacat ttttagtcaa gaatggaaac gtaactacat
     9001 aatcaaaggt tttgcgaaat atttcatgaa atatttcatg tagaagcgag caatacggtt
     9061 gcaaataacc attatcgttt aataagcagt tacgaaagct cccaatatct ctaacgttca
     9121 gctcactcca tttcttttct tcaaaaaaat cagtgattcg tatctctttc tatttgtcga
     9181 aaaagttaac tttaaaaagt aacgccgaaa aaatatgcaa aaaaataaaa aagcagttac
     9241 tattgctaga ataacatcaa ggaataataa gttttggttt tcagataata gtcgataagt
     9301 tgtgtattgg aattttagat acagtaataa agtatttttc atgaaatttt gtgagaatca
     9361 ctattacaag tatctttaat aaaattaatg gttaaggagg aacttaaagt agaatgtaaa
     9421 tgaatagttg tagtcagtcg attgtgatac tagactcaaa gttgcaggaa taagtttttc
     9481 aatttgtgat tggaagataa aaaatgaaaa agaagggctt ttttttatac tttgttgatt
     9541 gtagtgttta ttttccctct gtttagccaa gtaacatttt ctatgactca accagtttca
     9601 agtgaggaac atgataacct ttttcgtgct acagaagagt ttaagaaata tcaggaaaat
     9661 attgttccgg agactccatt agctagtgag ccaattactg atattgatgg aaaccaaata
     9721 ggatggtata tacagtacga agttatggtg gtaaataatg actcaatagt aaaaaataca
     9781 gtaggcttag agctaacacc gattgaaaag gtgagttcat tgttaacgta tatttatact
     9841 gatgaaagtg acgagactga gatcatttta acggattact caaaaattgc tactgataaa
     9901 aagtatatat tgtggatgta gacactagtg aaatacttta tgatatagaa attgatgaaa
     9961 atttactaga tgtagcacaa gaaatcgatc atcaaaagca ggaagctatt cgatatgctg
    10021 gtagaaaagg gaaaagaaaa tatttattac gtgaatgtag caaaagttag agaagatgaa
    10081 aatgagtgga aagaattcaa gagtcgctac tcaattaact cgactccgac atttacagtt
    10141 tacagagaag gatcgataga aaaaactgtt ttttggacta aagaaagtgg aatgtcttta
    10201 gctgaagtag aagagttttt ggattatgtt agtatgcaac agtaagggag ttcttattat
    10261 gaaaaatttt gacacatatt atgttattgt tttttcgata tctttgctgt taatattact
    10321 aactttaatg cagtataaag agtatccaga gtattttatt ttaacttttg tatatttagt
    10381 aagtagctac acacttctat ataaattcag aagttcttct agagtttcta aagtttgttt
    10441 cttaattagc ttattgattc tataatattt ttgatttata taggaaatta aaaatattat
    10501 agaatcaaca ctcagagaac gggttggttt ttaatagatt taataagtac tgataatgaa
    10561 atttctcctg gttgggaaaa cgaagttggt taccctgtag aagaattttt tcaagattac
    10621 tttgcggagt atgccgatag gataatgaga gattacgtaa cagataaaac tcaagaagtc
    10681 atcaatcaat cccagtcatt aaatgaatta tctgataagg cgaaagaaga agcagttgcc
    10741 attatttctt cagggattag tgactatatt gatatgagtg gtgaaatcaa ttatggcgat
    10801 aaagacaaaa ttgtacgcat gataaaagct tttatcaaaa cttttgatgt gaaggcagat
    10861 cttaagttta atgatactta cctagtagga aaattaatta atatccttgg gacagagaaa
    10921 gatacggaaa cgtttatttc ctctttgtct atgttgttta cagataagca tttttcaact
    10981 cagcttggtg gagaggaact agtggaaatc ttttaaccat tacgcccagc attaataaaa
    11041 gtatatcaaa aaagtactcc tgatgatttt taagattggt ttgagaaata atgtgaaaac
    11101 actattttta atgttaatta tagatagaga tgaacgcgtc atatggttaa aagaatgaaa
    11161 aagtcaaaga tacactgcta tatttcacta aggatatagc agtgtatctt tcatgtcgtt
    11221 tcactggcag ttttttataa aaggaggtgt gcatttcagt tgcttttctt tggtagttag
    11281 ttgaatcgtt taagaattaa cggatgtggc agacaggtgc tctgtccaaa aaccagtaaa
    11341 atctttgatg gttccatctt tatttagctg tgcctgttca aatactgttt tgtttttacc
    11401 gaatggatca attgcaataa ttgttccgat ccccgcacgt ttggcagcaa gtaatcctga
    11461 gtaagcatct tcgataacaa gacaatcagc cggagctaag ctcagtttgt ctgccgcttt
    11521 aagaaaaata tccggttgcg gtttcccagg gaaagagccg tcgtcataga caatcttgtc
    11581 ccagtcaaac cagcgggcta aatcgaaaat atcaaaataa aaagcaacat tctctttgac
    11641 agtagccgtg gcaatcgtta atggaatctg ttggtcagtc agctggtcaa gagtcgcttc
    11701 aagccctttt gtcaactgaa gctcgttttc attttgcaag caaagcatac gataatagct
    11761 ttctttttct tctgacaata cggcgatctc agaggcggtc aaatgctctg aaatgaaatg
    11821 tttcaaaatc tccgtattcg tccgaccatg aatatttttt aaaatatcct cgtctgataa
    11881 accatctttt gtatgatcat gtatcatatg cagccaagca gcttcatgca gatgagagtc
    11941 caaaaacatt gttccgttga aatcaaatat aatccctttc atagttacgc tcctatagtg
    12001 gtattttcgg ttgaatcagc aatttgcaga tcgacatatt gtgcatagtg ctcttgaacc
    12061 tcccgcgaga tctggcgatc cgtaatgagc agatcgaaat catgcaaatt ataaaacaca
    12121 taaaaatcat ctcgattgaa ttttttatta tcaacgagta gatatttctt acgggaatta
    12181 tttagagcga tctgctgtgc ttctccttct tcggtactgt aagtagagat cgcttcgttg
    12241 tgaacgccat ttgaactgat aaatgctttt gtaaacttta gtcttcttaa attgtcgttg
    12301 gtgatcggtc cgacaaatgc gccggtattt ttccggaaat caccgccaat cagtagaatt
    12361 tctggtccat ctgcttgagc aagaatatca aaaactggat aattatttgt aatgatccga
    12421 attttcttgt tcagtaaatg cgtcgccaat agttcgattg ttgtacctgg tcctaagaaa
    12481 atcgtttctc cctcgtgaat caaggaggct gctgtggcag caatttcttt tttttcttga
    12541 atctgcagag tggctttttc agtgtgagaa agttcttgat ctaaagtgta ggaaagactt
    12601 tgagcccctc catggacacg cagcagcttg cccgattttt ctaattcatc taaatctctg
    12661 cgaacggtca tatcagaaac atctaattga ttgatgattt cattaacggt aaggattcct
    12721 tttttattga ccatttctac gatagtaagc aatctttctt ttttcaacat tgtcttcacc
    12781 tctaatttaa ggataatcta tcatataaac ggacaacagt caatctaatt cgaacaaaag
    12841 taaacattta gggtggtttt tatgtttgtt acaatagatt ttattgctgt tttctggttt
    12901 ttatgattat ttgtgtttgt ttttgttgac aaatgttttg gatgcgttta caatgcaatc
    12961 agttagaaag agcgaaaact gattatagat ttgaggagag atgtacatgc ttaaattacc
    13021 agaagatttt atttttggcg gagccacagc agcctatcag gttgaaggag ccactaagga
    13081 aggtggcaaa ggcgctgttg cttgggatga ctttttagag gaacaagggc gattcagtcc
    13141 cgatccggca agtgattttt atcatcaata tgcaaaggac attgaattgt gtgaacgttt
    13201 tggtgtaaat ggtctaagat tatccattgc ttggagccgc atttttcctg atggagctgg
    13261 gaaaccaaat ccagaaggaa tcgcttttta tcatcgtgtg ttcgaagaat gtaagaaaag
    13321 aaacgtaacg ccgtttgtga cgttgcatca ttttgatacc cctaaacgtt tgtttgacca
    13381 tggagatttt ttgaatcgag aaacaataga agcatttgtt tcctatgcca ttttttgttt
    13441 tcatgaattc aaagaagtca aggtgtggag tacctttaat gaaatctatc cagttgcaac
    13501 caatcaatat ttgttaggtg ttttcccacc aggaatcaaa tatgatttta caaagatcgt
    13561 cgcctgtctt cataatatga tggttgctca tgcccgtgta gtgaactatt tcaaagaaaa
    13621 cgaacttccg ggtgaaattg gtgtagttca ctcattagag acgaaatatg cggcaacaga
    13681 cgcgccggaa gataagcatg ctgccttttt agacgatgca ttgtcgatcc gtttcttatt
    13741 agatgctacc tatttaggct attattcaac tgaaaccttg actgctcttg atgagatatg
    13801 tgaagcaaat caggccagct atcattttcc tgaagaagat ttcgttgaat tgaaaaaagc
    13861 atccacgcgt aatgactact tagggatcaa tcactaccaa tgccattttg tcaaagctta
    13921 cgatggagaa aatgcaatcc atcataacgg tacgggtgaa aaagggacct ctgtttataa
    13981 agtgaaggga atcggagaac gtatttataa agaaggaatt cctcgaactg attgggactg
    14041 gctgatttat ccagaaggtc tatatgactt gttgctgcga attaaaagcg attacccaca
    14101 ttacaacaaa atctatatca ctgagaacgg gatggggtat aaagatcagt ttgaagatgg
    14161 cattattatg gatcagccga gaatcgacta tttgagagtc tatttggaat ccttaagcaa
    14221 ggcgataaca gccggggtaa atgtgaaggg gtattttttg tggtcgctta tggatctttt
    14281 ctcatggaca aatgggtata acaaaaggta tggcttgttt tatgttgatt ttgaaaccca
    14341 gaagcgttac ccaaaagaat cagcttactg gtacaagttg gtcagtgaaa ccaaaacgat
    14401 tatttaataa gtgataatgg aggaagaaaa gatgaataaa gaggaattac aaatgcttgg
    14461 ttttgagatc gttgcatatt caggcgacgc tcgcagtaca ttattgaaat tattgaaaga
    14521 agtaagacaa ggaaactttg aacatgttga ttcggcgttg aaagaagccg acgaaaattt
    14581 aacccttgca cataattcgc aaacaaaaat tttagcggaa gaagcatctg ggaaagagat
    14641 ggagatggga ttcattttta tccatgggca ggatcattta atgaccactt tattgctgag
    14701 agatctgatt caagacttta ttgtactgta cagacagaat caaggatagg agaagaacgc
    14761 tatggatgga atcattaaac aaatcgaaaa agggaaacca ttttttgaaa aaatctcacg
    14821 taatagttat ttaggagccg ttcgtgacgg atttttaaca gcgatgccag ctattttatt
    14881 ttcaagcatt ttcattttga ttgctgcggt tcctgagatt tttggttacc aatggcctga
    14941 atctgtctcc acatggctat ggaaagtcta taattattcg atgggtgttg tagggttact
    15001 cgtttcagca accactgctc gctgcttggc tgattcgatg aatcgtcgct taccgaaaaa
    15061 caaagtaatc aacaccacct ctgtgatgct ggcctctgtg gtaagtttcc tgttgttaag
    15121 cgtctctccg attgaaggtg gattttcaac ggaatatatg gggacaaagg gactactggc
    15181 ttcctttgtc gctgcatttt taacagttaa tttatataag ttttgtgtgc tgaaagatat
    15241 taccattaaa atgcctaaag aagttcctgg aacgatttcg caaaccttcc gagatatttt
    15301 tcctttctct ttttcagtat ttgcggccgt tttaattgat ttaggcattc gacaaatctt
    15361 ccaaatctca tttgcagaag caatcattac ggtgctgcag ccgctattta cagcagctga
    15421 cgggtactta ggaatcgcta ttatctgggg agcgatggcg ctgttttggt ttgtcggtgt
    15481 tcatggaccg tcgatcgtgg agccggcaat tgcggcaatt atctatgcca atgtggaaac
    15541 caatctgcaa ttgttcaatt ccggtgaaca agcttctaaa gtactgacgg tgggtttagg
    15601 gaattttgta ggaactatgg gaggaactgg ggcaacattg gttgttcctt ttatcttcct
    15661 actctttgct aagtctaagc agttgaaggc agttgggaaa gcttccttta ttccagtaag
    15721 tttcgctgtc aacgaaccat tgctatttgc ggcacccatg attttgaacc cttatttttt
    15781 tattccattt ttgttaacac ctgttgtaaa cgtttgcctg ttcaagtttt ttgtcgatgt
    15841 attgggcatg aacagcttta tgtacgtttt gccttgggca actccagcac cgatcggttt
    15901 ggttctagga acgggcatgg gtatcttagc ttttgttcta gcagcggttc taatcgtcgt
    15961 tgacttcctg atctatctac cattttgcaa agcatacgat gctgtcttag tagagaaaga
    16021 agcacaaaaa gcagaggtga tcgcaaccga ggaaggtgct tctgccgatg tacgtgagga
    16081 agtacctgtg gcagcgagtc aagttatcag cgatgcaaat aaagaaatca atgtgttggt
    16141 actttgtgct ggcgcaggga caagtgcgat gttagctaat gctttaaaaa aaggtgcatc
    16201 tgaatcgaaa gcaccgatca gtgcaatggc gggtgcttat ggttctcatt acgaaatgct
    16261 gcaggatttt gatatggtgg tcttagcacc gcaagtcaat tcttactatg atgatatcaa
    16321 agaagacacc gatcgcttag gaatcaagct ggttgcgaca aaaggagcag aatatatcgg
    16381 attaacgaga gatcctgaaa aagctgttca gtttattctg aaccaatttc aataaaggag
    16441 gagccataat gaaaatggga atttggttgc gtaaagctgt tttatttgca gctgtgttag
    16501 gggctttggg ggctgtttca aaaactgttg aagcatctgc cagcgaattt attcgcggag
    16561 cagatatttc gatcctgtca gatatggaaa aatctggtgc aaaatattat gaagacgata
    16621 tttcaaaaga tgccttagag attttaaaaa ataatggtgt caactatgtt cgactgcgtt
    16681 tgtggcagga tccttataat agtaatggag aaagttatgg agcagggaca aatgatctgg
    16741 aaacgacgat tgaccttgca aaacgagcaa aaaatctggg acttaaggtg ctgctggatt
    16801 ttcactacag tgatttttgg gttgatcctg ggaaacagaa tcttcctaaa gcatggcaag
    16861 gtctgacctt tgaagaaatg aatacggcat tgtacgacta tacaaagaac gttctgtcgg
    16921 aaatgaagca gctggacgtt tatcctgata tggttcagat cggcaatgaa ctgaatagcg
    16981 gaatgctctg gccttatggc aagtcttggg gagaaggcgg gggagaattt gatcgattgg
    17041 ctgctttttt gaagtctggt atccaagctg ttcgagacac tcaacctaaa acaactccag
    17101 taatgctcca cttagctgat ggtggcgata caggagcatt tacttggtgg tttgatgaaa
    17161 tcacgagtcg aggtgtgtca tttgatttaa ttggagtgtc gtattatccg tattggcacg
    17221 gttcaatgaa tgaacttcaa accaatatga atgcgatcag ccaacggtat ggtaaagaag
    17281 tgatcgtagt tgaaacagct tatgggcata caacagcgaa tgccgatact atgccaaatg
    17341 cttttggtga agccgaagca gctgcggggg gatatgaacc aacaccgaca ggacaagcag
    17401 agtatttgct ggatcttgcc gatcggattc aggcagtacc gaataatcgc ggggcgggct
    17461 tcttctattg ggaacctctt tggtacaacg gaaatgtgag ttgggcaaca caagcaggta
    17521 tgaattatct cagtgttcaa tccactatgg gaaacgaatg ggacaaccaa gcgatgtttg
    17581 attttgctgg aaacgccttg ccaagcttgc gtgcgtttaa acaagcaggg gcgcaaataa
    17641 atctagttaa aaatgcaagt ttcgaaagcg atggaccaac gaccagtcct tccaattggc
    17701 aaacctggtt tcaacaagga accgctaagg gtacgatcaa aacggaagcg ggcacttttt
    17761 ttggggacta caaactaact ttttggcagg atcaagcata cgaggcatca gcttatcaaa
    17821 atgttagtgg cataagcaaa ggaacttaca cattgagtgc ctatgtaatg tctgggggag
    17881 agtttgatac taatcagtta tatatcaaaa attatggtgg agcggaacta aatactgcgg
    17941 ttgttaaaac aagtgaatgg acaaaaatta caatcaaaga cattccaatt acaacaggtg
    18001 tttgtgaaat tggggtttat agcaaggctt caggaaatgc ttggacgaac gttgattttg
    18061 tttcattggt gaaacaatag actgcggatc gtttcgattc aagatcggag gttcccttca
    18121 gccacatcgt tagcgtagaa ggcaggaatc gaacctgcac ctcgcctttg ccaaaaataa
    18181 ccagaaacga tttccagtca tttttaccaa aacgagtaaa gcaccagact tcatctacac
    18241 tttgtattat gctaaagaga gttcaacgat ttcgtaagaa gcaatgaaaa aattgcaaaa
    18301 agttgtgcag ataacataaa gtttgtttaa gaaagggtaa ccttagaatt ccgtcgtgaa
    18361 tatgatttga gcacttagat ggatagaaaa aagttctcgg aaaacagttc gtttttcgag
    18421 aacttctttt tatgataaga atgaaacaaa ttaacttata ttatagtctc ttttggtagg
    18481 gaaccatctt atttttaatg aaaatcaagc attttagcta ttgaagaatg ttgatttata
    18541 gggattcccc ctccatatta tgttggctct ttattcccac tccactgttg ctggtggttt
    18601 agaagtgatg tcatacacga tacggttgac gtgggctact tcgtttacga tacgtacaga
    18661 gattttttgc aagacttccc atgggatacg ggcaaagtca gcggtcattc cgtcgattga
    18721 agttacagca cggataccga ctgtgtagtc gtaagtacgt ccgtcaccca taacaccgac
    18781 agagcggatc cctggaagaa cagtgaaata ttgccagata tcacggtcaa gtccagcagc
    18841 tgcgatttct tcacgcaaga tagcatcaga ttcgcgtacg atttctagct tgtcttcagt
    18901 gatttcgcca agaacacgaa taccaagacc aggaccaggg aatggctgac gccaaacgat
    18961 tgaatcaggc atgcctaact gagtccctaa ttcacggact tcgtctttga ataatgtgtt
    19021 taatggttcg atcaattcaa attgcatatc ttctggcagt ccacctacat tatggtgaga
    19081 tttgatcgtc tgtgctgttt ctgtaccaga ttcgataacg tcagtataca acgttccttg
    19141 tgctaggaaa gagacgcctt cttcaccagc aagttttgtt gcttcgtcat caaaaacata
    19201 tacgaattca ttcccaatga ttttacgttt ttgttctggg tcagatacac ctttcaactt
    19261 gtctaagaag cgatctcttg cattgacttt gatgatgttc aagccgaatt ttccgcctag
    19321 actttccatt acttgttccg cttcgccttt gcgtagcaga ccgtggtcaa caaagataga
    19381 tgtcaattgg tcaccgatcg ctttttgtaa aagaacccca acgacactag aatcaacgcc
    19441 acctgaaaga ccaagcaaga cttttttgtc accaacttgt tcacggattt tttctacttg
    19501 catatcgata aagttatcca ttgtccagtt accttcacag tgacaaacat caaaggcaaa
    19561 gtggcgcagc aattcgattc cgtattcgga atgacgtact tctgcatgga attggatacc
    19621 ataaaaatta cgtgttgtgt cttggataga agcaatcgga caatctttgc ttgttccgac
    19681 tgtttcaaag ccttcaggaa ctttagttac caagtcacca tggctcatcc atactgtttg
    19741 ttgttttggt gtttctttaa acaagacagc ttgatcgctt gttacttcta acaaggcttt
    19801 cccatattcg cggtttttag caggctctac ggaaccgcca agtttgtacg tgatcaattg
    19861 cataccatag caaatcccta atacaggaat acctaaattg aagatctcag gatcgatccc
    19921 aaatgcattc tcatcatata cactgtttgg tccgccagaa agaatgattc cctttggatt
    19981 catcgctttt acttcttctg cagtaatgcg gtggctcaat aattcggaaa agactccaag
    20041 atcacgaata cgtcttgtga tcaattggtt atactgacta ccgtagtcta atacgataat
    20101 cttttcaacg ttcgtcatat cggcaacgtt tgtcaaattt attaccccta tccctttaaa
    20161 ttttatctca aaatagtcaa actattttaa aatgctaata ttttcgcatg aaaatcgaat
    20221 cgatgatatg attttcagtt ttatggagaa ttatatcggc tcttcctcgt gtaggcaaga
    20281 tatattcctg taaatttttc aaattcacat ttttccatac atcttttgcc attttaaagg
    20341 cttcttcacg atctccgata gcaaaacgat aataataatt ctctggattt tggaaagccg
    20401 tatccagtaa cgcaccaaaa cgatccaaat accatttttc gatcagcacg ggatcggcat
    20461 cgacatagac agaaaaatca aaaaagtcgc tgacgtagat ttgctgattc gcaggtaatt
    20521 gaagcgtatt gatcccctca acgatcaaga tatcaggctg ctggatcaat tcgtacttgt
    20581 ctggaatgat atcatacaca tcatgtgagt aaacaggtac ttttatctct tcctgaccag
    20641 attttacacg gttcaagaaa tcgatcagca tctccatatc ataactttca ggaaatcctt
    20701 tacggtccat gattccacgc tcttttaata tacgatttgg ataaagaaat ccgtcagtcg
    20761 tgatcagctg aacgtttctt cgtttgaagg tacgagacaa aatcgtctgc agcagtctgg
    20821 ctgttgtact ttttccaacg gctacacttc cggcgatccc gataataaaa ggcggtactt
    20881 ctacgtattt atgaagaaat aaacctttac tcaagaacaa agattcaaat tctttcatat
    20941 aaagataaat cagatgagca agaggtacat agatatctcg aacatcctgc atggaaatgc
    21001 ggtcattcaa acttttgata ttatctagtt catcttgtgt caaaggtgct tgcccattac
    21061 gataaaattc tcgccattct tctcttgcca cacggtagta gttcattgga tcattcatat
    21121 tagcctcctg cgttctccca tcaatggaac tattttaaca caaaataaac gcaaacccca
    21181 ccacttaaga gagattgccg atctttttgt ataatcttta taatagaaag aaacggagtc
    21241 ttattcttga cttgcgcacg gatacataaa aggatagact gttgtcatga aagaaatgag
    21301 aaggatggaa gacatgacaa atcattacta ttctgaacgc ccagatacgg cgcacgattt
    21361 tgaacaatgg gcttttgagt taaaaggcag aacctttcaa tttgtaacag attctggtgt
    21421 attttcacgt gaaacagtgg attatggttc acgtgtcctg attgatgctt tcgattggga
    21481 agagttacct gatgaaggaa agatcttaga tgtgggatgc gggtatggac cgattgggtt
    21541 agctttggca tttgcgacac agcggtttgt tgaaatggtc gacattaatg aacgagcggt
    21601 aagtctagcg caaggaaacg caaagcgtaa ccaaatcgaa catgttgata tccatcaatc
    21661 gaatatctat gaagcagttc atgaagaaac ctatgcagct atcgtaagca atccgccgat
    21721 ccgtgctgga aaaaaagttg tacatgaaat tttgacaggc gcatatcctc ggttgaagaa
    21781 gggcggtaca ctgacaattg tgatccagaa gaaacaagga gcgccgagtg cgcaaaagaa
    21841 aatggaagaa acatttggta atgcagaaat cgtcacaaaa gataaaggat attatatcat
    21901 caaaagtgtg aaagaataac tagctgaaaa atagtggagc gacgaaagag tggaactggg
    21961 cgtgcatgta cgggtacgta tcagttcttt ttttgttgag aagtttttaa aaacaaagaa
    22021 ctattttttc ttcaaaaaga aatagtagga acagagtcgt tgttttttac gataaaataa
    22081 ataagataca gaacttgtat agaaggctgc caagtcggta gataaaggag gtttttatca
    22141 aaaaacatac tttccttttt tctctattct tttcttgggg gttttggtac actgataggg
    22201 aatttagata gaagggagcc aataggatga agctgaccat cagagatatc gcagaaatgg
    22261 caggcgtatc agtaacaact gtctcacaga ttttgaataa caaaggaagc agatttagtg
    22321 ataagacaag aaaaaaagtg ttgtcgatcg tgaatgaaca tcattataag cccgattatt
    22381 tcgcttcaaa tgtgatcaat cgacattcaa agaccatcgg aatgatcgtc cctgatgtca
    22441 ctgatttctt cttctccaaa ctgatcgaag gtgtagagag ctatttgaat cctttgggct
    22501 atgtgatcat gttatgcaat tctcgtcata gccaagaaaa tgagatcaag tatttacaag
    22561 agctttccca ccgctcagtg gacggaatct tgctggccac accaaatatt ttacctgaga
    22621 aatatgcatt agacagcggt ttttatcaaa aaatgccaat cattttaatc gatcgcggat
    22681 tgaatcgtcg ggataacgga cggcttatcg taaaggaata tgagggcgcc tatcaagctg
    22741 tttcgctgtt tattgaaaat ggccatacga agatcggcat gctgaaggaa accactggat
    22801 actatcaatt agaagaacgt ttcaacggat atcgtcatgc gctgaaagat catggcctgc
    22861 cttttaatgg gaaatttgtt gaacatggag agttgactgt ccaaggtgga tatactgcat
    22921 caaaaaaatt actcaaacat aaagatatca cggctatttt ttgcgggaat gatgccatgg
    22981 cgatcggatg ctatcaggcg atcgatgaat tgggcaaaaa aatcccagaa gatatctcag
    23041 tcataggatt cgatggatta aagctgtcag aatacatgat tccgcaattg acgacagtgc
    23101 agcagccaag ttttgatatt ggattttatg ctgcacgttt cctgatcgat acgatcgaat
    23161 ttcctcaaag aaaagttccg aacaaagtgt tcgaaacaaa attaattatt cgtgagagcg
    23221 taaaagtatt gacaaagggc taactcggcg gtatattgct tatgtaagca tttacaaagg
    23281 ctagttagtg ctttttttat ttttagtaaa ccagtttact aaagtggttt acctttccta
    23341 aaaataatgt ataataccga atgaagacaa ggaggatcag gcaatgcgaa tggtggatct
    23401 gattgaaaaa aagagggatg gaaacgagct gtcgaaagag gaaatcgagt acatcgtcac
    23461 gaactatacg aatggaaaga ttccagatta tcaagtcagt gcattattga tggcaatttt
    23521 ttatcaagac atgacaaatg aagaaatcac gaacttaaca ttggcgattg ctaattctgg
    23581 tgatgtcatc gatttgagtt ctcttgaagg aataaaggta gataagcatt cgacaggtgg
    23641 cgttggtgac acgaccacat tgattttagc ccccctagta gctagtgtcg gagtgacagt
    23701 ggctaaaatg tctggacgcg gtctaggtta cacaggtggt acactagaca agctagaagc
    23761 aattcccgga tttcagatcg agctttcgga tgaagaattt gtacgcattg ttaatgaaag
    23821 taaagtagcg gtaattggtc aatcaggaaa cttggcaccg gcggacaaaa aattgtatgc
    23881 cttgcgtgac gtcacagcga ccgttgattc tcttccgctg attgccagtt cgattatgag
    23941 taaaaaaatc gcggcaggag cagatgcaat cgttttggat gtgaccactg gagacggtgc
    24001 tttcatgaag aacatcgaag atgcaagacg tctggcaaag accatgacga gcattgggaa
    24061 actggctaac cgagaaacag tggcggttat ctcagatatg tcagaacctc ttggcgaagc
    24121 tatcggaaat agtctagaag ttgtcgaggc aatcgaaaca ttacaaggaa atggtccaga
    24181 agatctagta gaaatgtgct acgccttagg aagccaaatg gttgttcttg ctggcaaagc
    24241 taagacaacc gatgaagcaa gaacgttgct tcaagaagct ctggaatcag gtaaagcgtt
    24301 agcaaaattc aaagaaatga tccaaaatca agggggagac ccagcgatcg tggaacatcc
    24361 agaacgaatt ttgactgcac gctacacaat ggagcttcct gctaaacaaa gcggagttgt
    24421 gtcaaaaatc gtggcaaatg aattaggaat tgctgcaatg atgctaggtg ctgggcgtaa
    24481 aaccaaagaa gaagatattg atcacgctgt tggattgaaa ctgcataaaa aaattggtga
    24541 cactgtgaca aaaggtgagt cactattgac gatctatagt aacgacgaag aaatcacttc
    24601 tgtcattgaa ttattatata aaaatattga aattggtgaa tcggcaatga agccaacgtt
    24661 gattcatgac atcatcacag aataaaggaa aaaagcggac tttaccgttt taaagaaaag
    24721 gggaaaagaa atggaactaa accgaatgat cgaccatact attttgaaag cagacgcatc
    24781 taaagaagct gtgatgcaaa ttatcgaaga agcaaaaaaa taccattttt attctgtctg
    24841 catcaatcca gcatgggttg cgctagcgaa agaacaatta caaggtgagc cagtagccgt
    24901 atgtacagta atcgggtttc ctctaggagc aaacacaccc gagacaaaag catttgaaac
    24961 aacagatgcc atcaataacg gtgcagatga agtcgatatg gtcatcaata tcggtgcttt
    25021 gaaatcaaag gatgatcaac aagtccaaaa agatatcgaa gcagttgttg aagcagcaaa
    25081 agatcgtgcg cttgtcaaag ttattattga aacatcgtta ttagatagag aagaaatcat
    25141 ccgagcttgc gaaatagcaa aagctgcagg cgctgatttt gtcaaaacat caactggttt
    25201 ctcaacaggc ggagcaaagg tagaggatgt ccgtttgatg cgtgaaactg taggaccaga
    25261 aatgggtgtc aaagcttccg gcggtatcca taacgaagaa gaagcaatgg caatgatcga
    25321 agctggtgct acaagaatcg gaacaagcgc aggagtagca atcgtatctg gttcaactgg
    25381 tgaaggctat taattttttc aaaggagaag ctcatggcag aactcaaaca agaatggatc
    25441 aacacagcta ttgaagcgtt agataaagcc tatgttcctt attcccattt tccggtaggc
    25501 gcttgtctcg taacggaatc aggcaagatt tatcaaggca tcaacattga gaatgcatca
    25561 tttggattaa cgaattgtgc cgagcgcaca gcttttttca aagcagtatc tgaaggagaa
    25621 aaaacattta cgcatctagt cgtagcgggg cacacccccg atcctatctc tccttgtggt
    25681 gcatgtcgac aagtaatggc agaattctgt tcccctgata tgccggttac gctagtagga
    25741 gacaatggtg tgacgaaggc aacgaccgtt cgtgcactgc tgccttatgc atttactgaa
    25801 aaagatctat aactcatgtt cattcagtca gctgactgtt gaaaaataaa acaattattt
    25861 taggaggctt tacagaaatg aaaaaagcaa aattatttgg tttaggtgct gtcgcactag
    25921 cagcagggtt atttttaggt gcatgcggca acaacggctc tactgattca tccggtggca
    25981 aaagctcatc agacacaaca actgcagcat tgatcacaga cacaggtggt gtagacgacc
    26041 gttcattcaa ccagtcagct tgggaaggac tagaaaaatg ggggaaagat caaggacttt
    26101 cacgaggaaa tgatggcttc caatatttcc aatcttcaaa tgaatcagat tacatcccaa
    26161 atattgacca agcattgaat gctggattca aaacgatttt tggtattggt tataaactaa
    26221 aaccagcgat ccaagaacaa gcaacaaaca atacaggtac gaactttgtt attatcgatg
    26281 atgtcatcga tggattagat aacgttgtat ctgcaacttt caaagacaac gaagcttctt
    26341 atcttgcagg tgttgcagca gcttatacga ctgaaacaaa cgtagtcggc tttatcggcg
    26401 gtgtaaaagg tgaagtaatc gatcgttttg atgctggatt caaagccggt gttgatgcag
    26461 gtgcgaaaga attaggaaaa gaaatcaaag tattgaatca atatgctggt gacttctctg
    26521 ctccagataa aggacgttca attgctcaag gtatgtatgc acaaaacgca gatatcatct
    26581 tccatgcttc aggcggtaca ggtaacggtg tattccaaga agcaaaatca ttgaacgaat
    26641 caggcgacaa aaaagtttgg gttatcggtg ttgaccgcga ccaatcagac gaaggcgaat
    26701 acacattaaa cggcgaaaag aaaaacttca cattgacatc tacactgaaa gcagttggaa
    26761 cagtagtaga agatttggca caaaaatcag cggacggtaa attccctggc ggcgaacaca
    26821 ctgtttatgg attgaaagaa gacggtgttg gtctaacaga aggacaactt tctgacgaag
    26881 cgaaaaaagc agttgatgaa gcaaaagaaa aaatcatttc aggtgacgta aaagttccag
    26941 aaactcctga agaaaactaa aaccatttct atagccttgt tagacacatg tttgacaagg
    27001 ctatggtttg attagaacaa gaattgttca tttatccaag ctgccttgtg acagtttgga
    27061 taagggaaca actccctgaa gtaatgtttg tttcaaaaga agaacgctca ctaagcgtga
    27121 atcttagtca atcgttgccc tagatagaaa tcgttaggat gtgaaaaaaa ggtggttcaa
    27181 gaaaatattg tcatagaaat gcagaatatt accaaagaat ttggaacatt caaggcaaat
    27241 gacaacatca atttacaagt aaaggcagga gaaattcatg cgttgcttgg agaaaacggt
    27301 gctggcaaat ctacattgat gaacgtgctt tccggattat tagagccgac atcagggaaa
    27361 attttgatgc gtgggaaaga agtaaagatc acaagtccga ccaaagccaa ccaattaggg
    27421 attgggatgg tccatcagca ctttatgctt gttgatgcct ttactgtaac agaaaacatc
    27481 gtgttgggaa gcgagcctag tcgcgcaggg atgcttgacc ataaaaaagc acgaaaagag
    27541 atccaaaaag tttctgaaca gtatggattg tcagtcaacc cagatgctta cgttcgtgat
    27601 atttcagttg gaatggaaca acgggtagaa attttaaaaa cactttaccg aggagcagat
    27661 gtactgattt ttgatgagcc gacagctgta ttgacccctc aagaaattga tgaattaatc
    27721 gtgatcatga aggaattagt caaagaaggc aagtcgatca ttttgattac gcataagtta
    27781 gatgaaatca aagcagtagc tgaccgctgt acagttatcc gccgtggaaa aggaatcggt
    27841 acagtcaacg ttaaagacgt aacctcacaa caattagccg atatgatggt tggaagaacg
    27901 gtttcattca aaacgatgaa aaaagaagcg aagcctcaag aagtcgtttt gtctattgaa
    27961 aatctagtgg tgaaagaaaa tcgtggatta gaagccgtga aaaacttgaa cttagaggtt
    28021 cgtgctggcg aagtacttgg tatcgctgga atcgatggaa acgggcagtc ggagttgatc
    28081 caagctttga ctggtttgcg aaaggcagaa agcggacata tcaagctaaa aggggaagac
    28141 atcactaata aaaaaccccg aaaaatcact gaacatggtg taggacacgt gccagaagac
    28201 cgtcataaat acgggttagt cctagatatg acattatctg aaaatatcgc actacaaacc
    28261 tatcatcaaa aaccttacag taaaaacggc atgctgaatt attcagtaat gaatgaacat
    28321 gccagagaat tgatcgaaga atatgatgtt cgaacaacga atgaacttgt tcccgcaaaa
    28381 gctttatcag gcggaaatca gcaaaaagca attatcgctc gtgaagtcga ccgagatcct
    28441 gatctgttga ttgttgcaaa tccaactcgt ggactggatg taggtgcgat cgaatttatt
    28501 cataaacgtc tgatcgaaca aagggacaaa tacaaagcag tgttactgat cagtttcgaa
    28561 ttagaagaaa ttttaaatgt ttcggatcgt attgctgtta tccatgaagg agaaattgtc
    28621 ggtatcgttg atccgaaaga aacatctgaa aatgaattag gtctattgat ggcgggctat
    28681 accttagaag aagcacgaaa agagttagaa aagggggctg gcgcacatga atgatcgcag
    28741 cgaaaaattc cgtaatttat tagtgcctgt actttcagtt gttttaggat tgatcttagg
    28801 cgcgatattg atggctgctt ttggttttca gcccatccaa ggttattctt ctatgctaga
    28861 tgcagccctt ggttcgcaaa gagggatagg tgaaacgtta aggcaagcaa cgccgttgat
    28921 ttttacagca ttaggttttt ctgtagctaa ctcagctggg tttttcaata ttgggctttc
    28981 tggacaagca ctttgcgggt ggatcgtcag tgtctgggtg gcacttgcgt ttccagatct
    29041 gccaaaagtt gttttgcttc cgttatgcgt gatcttaggt gccttagctg gtgcgatggc
    29101 agcggctgtt ccaggtttac ttcgagcatt ttttggaaca agtgaagtga tcgtaacaat
    29161 catgatgaac tatatcttat tatacgtaag cacatttgct ttacatgaag tgatgcctga
    29221 aagctatcgc tcaagtttgg actcttccaa tatgatcagt caaaatgcct cactgcggtt
    29281 accatggtta acacaagctt ttggcggctc tcgcgtaaac tcaggattat ttttagcaat
    29341 catcgcgctt attttcgttt ggctgctgat gaagaaaacg actttaggat ttgaaatccg
    29401 ttctgttgga ttgaatcctt ttgcttctga gtatgcagga atgagtagta aacgtacgat
    29461 cgttttgtcg atggtcattt ctggcgcttt ggcagggctt ggcggtgtgg ttgaaggatt
    29521 aggaacgtat caaaacttct ttgttcaaac gacttcgcta tctattggat ttgacggaat
    29581 ggctgtttcc ttgcttggtt ctggatcagc aatcggtatt cttttatcag cgatcttgtt
    29641 cagtattttg aagatcggtg gacttggcat gcagacagga gcaggtgttc cgtttgagat
    29701 cgtcaatgta tcgattgctt tgattatctt ctttgtcggg atcaacttcc tgattcgttt
    29761 cgtcatggct aaagtcttcc aagggaaaaa acaagaagag atcgttgcga caatagaatc
    29821 gaaaccaacg aatcaaaacc atgaaggggg cgaactataa tgtcgactat cgaattaatt
    29881 gccttgatcc ttacttctac actggtttat tcaacaccgc taattctaac gtcactaggc
    29941 ggaacttttt ctgaacgaag cgggatcgta aacgtagggc tagagggaat catggtcatg
    30001 ggtgctttta gttccatcgt tttcaacttg acgatgagtc catcacttgg ttctatgaca
    30061 ccatggatcg ctattttagt gggtggcgtc gtaggtatcc tattttctct gattcacgca
    30121 gtagcaacag tcagtttgcg tgctgaccat attatcagcg ggacagtaat taatctgatg
    30181 gcacctgctt taggtgtttt cttgatcaaa gcatggtatg gtaaaggtca aactgataac
    30241 atcacgcaga atctggggta tttttctttc ccaggattag cagatattcc ggtcattgga
    30301 ccaattttct ttaaaaatac attcttacct gcttattgtg ctattttgat tgctgtccta
    30361 gcttgggtga tcatcttcaa gacgaaattc ggcttgcgcc tccgttcagt tggtgaaaat
    30421 cctcaagctg ccgacacatt aggtatcaat gtatatgcta tgcgttattc aggcgtactg
    30481 atttctggct tgttaggcgg tatgggcggt gctgtttttg ctcagtcgat ttccggtaac
    30541 ttttcagctg gtacgatcgt tggacaaggg ttcatctcca tggctgctat gatctttggt
    30601 aagtggaatc ctttaggggc aatgggtgcc tcattgttct ttggttttgc tcaaagcttg
    30661 agtatcattg gcgcacaatt accagttatt tcttcgattc cgccagtatt gctgcaaatt
    30721 gctccatatt tgctgacaat cattgtgctg gttgttttct tagggaaagc ttctggtcca
    30781 aaagcaaacg ggaagaacta tatcaaatca aaataatctt ttagcgaata aaaagggtgg
    30841 gttacgggaa gtgtttttcg tctcgccctt tttgttttaa aggcaataaa aataaaaaga
    30901 aagaaggaat aatcatgttt aaacgcgtac atctgatcgt catggattca gtaggaattg
    30961 gagaagcacc agatgccgat aagttcggtg atgttggttc tgatacatta ggacacattg
    31021 cgaaagaagc aggattgaac gttccacatt tagagcagct tggtttagga acgatccgtc
    31081 ctcttgaagg tgtggaatca gtgaaagatc atcaaggcta tgcgacgaaa ttagaagaag
    31141 tttctgtagg gaaagatacg atgacaggcc actgggaaat catgggattg aatatcaaaa
    31201 ctccatttcg tgtgtttcct aatggattcc ctgatgatct gatcaaacaa atcgaagaat
    31261 tttctggccg taaagtcgta tgtaacaaac cttatagcgg aacagctgtt attgacgatt
    31321 acggtgaaca ccaaatgaaa acaggcgatt taattgtata tacttctgcc gatcctgtcc
    31381 ttcagatcgc ggcacatgaa gaaatcattc ctttagaaga attatatcgg atttgccaat
    31441 tcgttcgtga tataacaaaa gatgatccgt atatgattgg ccgaatcatt gcacgtccgt
    31501 acgtgggtga accaggagac ttcacacgaa caagcaatcg ccatgattat gcgttgaatc
    31561 cattcggaca cacagtcctt gattctttga aagaagcagg aaaggacgtg attgctgttg
    31621 gaaagatcaa cgatattttc aacggacaag gaatcactga atctgtccgc acgaaaagca
    31681 atatggatgg cgttgaccag ctgctgaaag ttatgaaaaa cgatttttca ggaatcagct
    31741 ttaccaattt agtagatttt gatgcattat acggtcatcg tcgggatgta gtcggctatg
    31801 cgcatgcgat tgaagaattt gacttacgaa ttccagaatt gcttgaagcg atggaagaag
    31861 acgacttgct aatgattaca gccgatcacg gaaacgatcc gacgtttact ggtaccgatc
    31921 atacgcgaga atatgtaccg ttattggttt acagtaaaaa aatgaaagaa caaggggcat
    31981 tgcctcaagg atattattca gatatttcag caacgattgc tgagaacttt agtgtttctt
    32041 cgactgaaaa tggcgaaagc ttcttgaaat tattaaaata atctttttaa ataggagaaa
    32101 gcaaatgacc aagttaagcg agatgttaca agaaacgacc cagtttatcc ttgataaagg
    32161 agtaaaagaa attgattttg gtttgattct aggttcagga ttaggtgaat tagcagatga
    32221 aatcgaagat gctgtaatga tttcttatga tgagatcccg ttctttcctg tttcgacagt
    32281 agttgggcat gcaggaaagc tagtttatgg gtctctggca ggcaagaaag tgttagccat
    32341 gcaagggaga ttccactact atgaaggtca ttccatgcaa acagtgacct atccagtacg
    32401 tgtgatggcc ggactagggg cccattcagt cattgtgact aatgcatgtg gtggtgtcaa
    32461 cgaatcattt gtaccaggag atttgatgct gattaccgat catatcaatt ttactggtga
    32521 aaatcctttg atcggtccga atgaagatgc aatgggccct agatttccag atatgagtca
    32581 agcttatacg cctgactatc aagcaacagc aaaagcagca gcagaaaaac taggtttgga
    32641 tcttaaagaa ggcgtatata tgggctattc tggaccaact tatgaaacgc ctgcggagat
    32701 ccgaatgtct cggacaatag gagccgatgc agtagggatg tcgactgttc cagaagtcat
    32761 cgtagcagct cacagcggat taaaagtgtt aggaatctct tgtattacca acttagcagc
    32821 aggtatgcaa gcgaatctga gccatgaaga agttgtagaa acaactcaga gagtcaaaca
    32881 atcttttaaa gcattggtta aagaaacatt gacattattg taataggagg aacaataaaa
    32941 tgagtgtgca tattgaagcg aaagaaggcg agatcgccga taagatttta cttccgggag
    33001 atcctttgcg agctaaatat attgcggaga cttttttgga agatccagtt tgttacaatc
    33061 aggtacgcgg tatgttaggc tttaccggca aatataaagg agaacgtatt tctgttcaag
    33121 gaacaggaat gggaatgcca tcagccacca tctatgccca tgaattgatc cagtcgtatg
    33181 gtgtaaagaa attgattcga gtaggtacat gcggtgcatt gtcaaaagac gtccatgttc
    33241 gtgatttagt actagcacaa ggtgcagcga ccagttcttc gatgatcgaa aaaaatttcc
    33301 aagcctttca ttttcctcca atcagtgatt tcaatctatt actgaaagca tatgagatag
    33361 ctaaagaaaa gggctatacg gttcatgtag gaaatgttct ttcggaagat tctttctaca
    33421 aagatgattt gacagagacg ttccaattag ctgaacttgg cgttttagga gtggaaatgg
    33481 aagcagcggc tctttattac ttaggcgcga aataccatgt gcagacatta agtttgatga
    33541 ccgttagcga ccatttgatc accggtgaag aaacaacagc tgcagaacgt caatctacgt
    33601 tcaatgaaat gatcgaagta ggattggaaa cagcgatagc agaataagaa ggaaatgaaa
    33661 aagctgccct gcattaagta ataagaacat ccaagcaaaa atagctcctc atgtttttga
    33721 cttggctgga cttattattg caaatgtaag cttttgaaca ccgtttacca agaggttgtg
    33781 aatctgccgt ttaactctga gtcacaagga acaatttcaa aaaacagctt ttcatgtttg
    33841 atgaatattg tgtcttgtga ccgagaagtt ggcaggagaa aaccgtttat acgaaaaaag
    33901 ggactgtgac aaatcgtgtc acagtccctt ttttatagtg ttttttatag gtagtatttt
    33961 ttagcaactg ttaagtcatc atttaattca taaacaagtg gttgacctgt tggaatttca
    34021 agatccatga tgtcttcgtc agagatacct tcgatatgtt tagctagggc gcgtaatgag
    34081 ttaccatgtg cagctactaa aacagttttg ttgtctagta atgctggagc aatttcatct
    34141 tgccagaatg gcaaagcgcg ttctaatgtc actttcaagt tttctccacc aggaatatcg
    34201 cgtttgtcta acattgcgta acgacggtca tttgctgcag aaccttcgtc agtttcttcc
    34261 attaatggag gaagagtatc gtaagaacga cgccagatgt gcacttgttc gtcgccgtat
    34321 ttttcagctg tttctttttt gttcaaacct tgtaatttac catagtgacg ttcgtttaag
    34381 cgccatgatt tgatttgtgg tacccaaagt tgatctgaat attctaagat caagttacat
    34441 gttttgattg cacgagttaa aacagaagtg taagcaacat caaattcgat tccagcttct
    34501 ttgattttgc gtccgccttc ttttgcttct tcgattcctt ctggtgctag atttacatca
    34561 gcccaaccag taaattggtt caatgcattc cattcactaa gaccgtgacg agaaaaaact
    34621 aattttggca tgtttagctc tccttttaag ataggtttat atttctgaaa ttttcagttc
    34681 tgttttattg tacaattttt tggaagagat ttccatcgaa aaattagata agattctatg
    34741 agaaaacaag tagagaatat aaaaaaacga acagtgccca cgcaactgtt cgtttttagg
    34801 tattagatgc ataattgctc gatttctttt tcaggtaaat tgatatattt ataacatgtt
    34861 ccaactgaaa caccgcattt tgaagaaata aattggatcg tttctttttt ttcataatag
    34921 agacgtcgga tttttttgat cgtttttgca tcgatttttg gtcttccgcc gatttttcct
    34981 tttttacgag cttcatctaa tccaactaat gtacgttctt tgatcaatgc gcactccatg
    35041 tttgctaggt tttccattaa ttggaaatag atttttccca tttcgcgcgt atcgattcct
    35101 tcatcaagac taatcaagtg cagcccgcga tctttgaata attgcgtgag ttctgttaat
    35161 tgccgcgtag atttgcctaa tcgatgaagt tggcaaatga ctagtgcatc gccttgttgc
    35221 atgtcagcga tcactgactc caaatgaaca gcagtagaat cattttgttc cacttcatat
    35281 gtctcatgga aaatttcttg acaaccaaag tcagataatt gatcaacctg agttgttaaa
    35341 tcaacgtcat ttatggttgt gcgtgcataa ccaataattt tcatggtgat tcctttctag
    35401 cagtgatttt ctaactggat ataacagtaa aaatgtagca taaagtacgt caaaaagata
    35461 gttttttcgc aaattttccg ctgtcttttt ttattggatg atcaagcagt ttttctttat
    35521 ttacgacgga ttgcgtcaag aatctcaacg cctttttctg agccgacaac tactttagaa
    35581 accatgttta ggaaaagccc atgttctact accccaacaa gttcatctaa ataggtagct
    35641 aagctttctg gctgttcgat gacttctaga tgcagatcaa tgatgtaatg accaccatct
    35701 gtggttaaga cttctccgtt ttcgtctgta cgcaagacgg gttggaatct tttttcttca
    35761 aaaatatgaa gtagctgttg ggaaccataa gggatcactt caactgggag tgggaacttc
    35821 ccaagtttat cgactaattt agaagaatca acgatccaaa tcgtttcttt tgagtacgta
    35881 gcgactattt tctcgaaaag caaagcagcg ccgccgcctt tgatcccttg aaaatcagca
    35941 ctgatttcat cagcaccgtc tattgtcaaa tcgactacgg ggacttcatc aatactcttt
    36001 aacggtattc ctaatttttc cgcttgttct tttgtcgcat ttgaagtagt aactcctgtg
    36061 atacgtaaac cttcttcacg catacggcga ccaatctctt ctaccatata ataggcggtt
    36121 gaaccggtcc cgagacctac gatcatcccg tcctttacga atttcgcggc ttcaatgcca
    36181 gctaattgtt ttaaattcat ctttgcgtcc accttttttt agtttgagca tcgtgctttc
    36241 ctcactattt tagcgtgatt tacggtaaaa agataccatt cttttttatt ccaataaagc
    36301 tttttcaaaa aaaattgcat ctcttgttga catgtttttt cattcgtgat attctaacaa
    36361 aggtgcttta tctacaggtt tctgtgaaga aacgtgctga ctgtttatca aagcatattt
    36421 gataaggatg cagacattgc atttattttt tatcacaaaa aaagaaccac ctggatgtgt
    36481 ggaactagac gcaaatcgtg ggaaggagga aacaaggaat gcctacaatt aaccaattag
    36541 tacgtaaacc tcgtaaatca aaggtggaaa aatctaattc acctgctttg aacaaaggat
    36601 ataacagctt taagaaaact caaactaacg taaactcacc tcaaaaacgt ggggtagcaa
    36661 ctcgtgtggg tacaatgaca cctaaaaaac cgaactcagc tttacgtaaa tatgcacgtg
    36721 ttcgtttgtc aaacttaatt gaagttactg cttatatccc agggattggt cataacttac
    36781 aagaacacag tgttgtatta ttacgcggtg gtcgtgtaaa agacttacca ggggtacgtt
    36841 accatatcgt tcgcggtgct ttagatactg ctggtgttaa cgaccgtaaa caaagccgtt
    36901 ctaaatacgg aactaaaaaa cctaaagctt aattttaatt attaaacaaa tgaaacttgc
    36961 ttctactata tagaagtaat tactatgaac aatatttcgg aaggaggagt tacggatgcc
    37021 acgtaaaggt cctgttgcaa aacgtgatgt tttaccagat cctatttata actcaaaatt
    37081 agtaactcgc ttgatcaacc gtgtaatggt tgacggaaaa cgcgggattg cagctaacat
    37141 tatctataat gcctttgact tgatcaaaga atctacaggt aacgatccat tagaagtgtt
    37201 tgaacaagca atgaaaaacg ttcaacctgt tcttgaagtt aaagcacgtc gtgttggggg
    37261 ttcaaactac caagtaccag ttgaagttcg ccctgaacgc cgcgttactc taggtcttcg
    37321 ttgggtagta aactacgctc gcttgcgcgg tgaacacaca atggaagaac gcttagcaaa
    37381 agaaatcatg gatgctgcaa acaacacagg tgcttctgtt aaaaaacgtg aagacactca
    37441 taaaatggct gatgccaacc gtgcgtttgc tcactatcgc tggtaagatt ctctctgttt
    37501 ggtcggcttt tataagtcga cctcagatag tgtccttatt tttagcaact agatagtaaa
    37561 gagaggagaa acaaggagaa tgaccagaga attttcttta caaaacactc gtaatatcgg
    37621 tatcatggcc cacatcgatg ctggtaaaac gactgctact gaacgtatct tgtactatac
    37681 tggtaaaatc cataaaattg gtgaaactca tgaaggagct tcacaaatgg actggatgga
    37741 acaagaacaa gaacgtggta ttacgatcac ttctgctgct actacagcac aatggaaggg
    37801 tcaccgaatc aacatcatcg acacaccagg acacgtagac ttcacagttg aagttgaacg
    37861 ttcattgcgt gtacttgatg gtgctgtaac tgttcttgat gcacaatcag gtgttgaacc
    37921 tcaaactgaa acagtttggc gtcaagcaac aacttatggt gtacctcgta tcgtatttgc
    37981 aaacaaaatg gacaagatcg gtgctgactt cttatattca gtatcaactt tacatgaccg
    38041 tttgcaagca aacgctcacc caatccaatt accaattggt gcggaagacg acttcacagg
    38101 aattatcgac cttgtaacaa tgaaggctga aatgtataca aacgatcttg gaacagagat
    38161 cgaagaaaca gaaattcctg aagaataccg cgaattagct gaagaatggc gcgaaaaatt
    38221 ggttgaagct gtagctgaaa ctgatgaaga acttactttg aaatacttag aaggtgaaga
    38281 aatcactgaa gctgagttaa aagaaggtat ccgccgcgca acagttaacg tagaattcta
    38341 cccagtacta tgtggttcag catttaagaa caaaggggtt caattactat tggacgctgt
    38401 tcttgattac ttgccatcac cgttagatat cccagctatc aaagggattg accctaaaac
    38461 tgatgaagaa gtagaacgtc ctgcagacga cagcgctcct ttctcagctt tagcatttaa
    38521 agttatgact gacccattcg taggtcgttt aactttcttc cgtgtgtact ctggtgtttt
    38581 acaatcaggt tcatacgtac aaaacgcaac aaaaggcaaa cgtgaacgtg ttggtcgtat
    38641 cctacagatg catgccaact ctcgttcaga aatctctgaa gtttacgctg gagatatcgc
    38701 tgctgctgtt ggtttgaaag acacaactac tggggacaca ctatgtgatg aaaagagtct
    38761 agttattctt gaatccatgg aattcccaga accagttatc caagtcgcta ttgaacctaa
    38821 atcaaaagct gaccaagata aaatgggtgt ggctttacaa aaactatctg aagaagatcc
    38881 tactttccgt gctgaaacaa acgtggaaac aggcgaaaca atcatcgctg gtatgggtga
    38941 acttcactta gatatcatcg ttgaccgtat gcgtcgtgaa ttcaaagttg aagctaacgt
    39001 aggtgcgcct caagtatctt accgtgaaac tttccgtgct ggcactcaag ccgaaggtaa
    39061 gtttgtacgt cagtctggtg gtaaaggtca atacggtcac gtatggattg aatttacacc
    39121 aaatgaagaa ggtgctggtt tcgaatttga aaatgcgatc gtcggtgggg ttgttcctcg
    39181 tgaatacatt ccagctgttg aaactggttt gaaagatgct atggaaaatg gtgtcttagc
    39241 tggctatcca ttagtagata tcaaagctaa actttacgat ggttcatacc atgatgtcga
    39301 ttcaaatgaa actgccttcc gtgttgctgc ttctatggca ttacgtgcag cagctaagaa
    39361 agctaatcca gtaatccttg aaccaatcat ggctgtagaa gttgttatcc ctgaagatta
    39421 tttaggtgat gttatgggtc acgtaactgc tcgtcgtgga cgtgttgaag gtatggaagc
    39481 tcgcgctggc ggccaacaag ttgttcgtgc gatggttcca cttgctgaaa tgtttggtta
    39541 tgctacaact cttcgttctg ctactcaagg tcgtggtaca tttacaatga catttgacca
    39601 ctacgaagat gtaccaaaat cagtacaaga agaaatcatt aagaaaaacg gcggaaaagc
    39661 tgaataattt cttaaaaccg gatagtcctg caaagagatt atccaaagag actatatttt
    39721 taatatagtt tcgtgtaaaa taattatcga tgatatgggc atgtggtgta ccttgtgtac
    39781 atcatatacc tatcagacaa ataaaaaata tattgtattt ttaggaggac attttaaaat
    39841 ggcaaaagaa aaatttgacc gttctaaacc acacgttaac attggtacta tcggacacgt
    39901 tgaccatggt aaaactacat taacagctgc aatcacaact gtactatcta agaaaaacgg
    39961 cggccaagct atggcttacg atcaaatcga tggtgctcca gaagaacgcg aacgcggaat
    40021 cacaatctca acagctcacg ttgagtatga aacagataca cgccactacg ctcacgttga
    40081 ctgcccagga cacgcggatt acgttaaaaa catgatcact ggtgctgctc aaatggacgg
    40141 agctatcttg gtagtttctg ctgctgacgg cccaatgcct caaactcgtg aacacatcct
    40201 attgtctcgt caagttggtg ttccttacat cgttgtattc ttgaacaaag tagacatggt
    40261 tgatgacgaa gaattactag aattagttga aatggaagtt cgtgacctat taacagaata
    40321 cgaattccct ggtgacgatg ttcctgtagt tgctggatca gctttgaaag ctctagaagg
    40381 cgacgcttca tacgaagaaa aaattcttga attaatggct gcagttgacg aatacatccc
    40441 aactccagaa cgtgacaacg acaaaccatt catgatgcca gttgaagacg tgttctcaat
    40501 tactggacgt ggtactgttg ctacaggtcg tgttgaacgt ggacaagttc gcgttggtga
    40561 cgaagttgaa gttgttggta ttgctgaaga aacttcaaaa acaacagtta ctggtgttga
    40621 aatgttccgt aaattgttag actacgctga agctggagac aacattggtg ctttactacg
    40681 tggtgttgca cgtgaagaca tccaacgtgg acaagtttta gctaaaccag gtacaatcac
    40741 acctcataca aaattctctg cagaagtata cgtgttgaca aaagaagaag gtggacgtca
    40801 tactccattc ttcactaact accgtccaca attctacttc cgtacaactg acgtaacagg
    40861 tgttgttgaa ttaccagaag gaactgaaat ggttatgcct ggcgacaacg taacaatgga
    40921 agttgaatta atccacccaa tcgctatcga aaacggtact aaattctcaa tccgtgaagg
    40981 cggacgtaca gttggtgccg gtgtcgttac agaaatcaaa gcttaattca cttaagtttt
    41041 aaagtaaagc aacatttatt cggacgtaat cgctcggaac gaaggtctca ggatctttgt
    41101 tccgagcttt tttatttatt gcaggatcag gaaaaaagac aggagtatta taatagaagg
    41161 aacgcgaaat aaaagagtca gtattttcta aatgaaactt tatgaaaaca aaatgttttt
    41221 tacaagaaaa agccttgctt tgtgagttta tatatattat aatgacaaag gtgcgtttac
    41281 tttagtgaat tgcacagacg taatcagaat gatttcgtcg gctaagaaac gagaggttgc
    41341 gacacgcccg gacgctttgc cacgaacgag cgtgacggaa attttcgtgg agctatgtct
    41401 acttttcaaa ataggcgaag gagggaacaa gatggcaaaa caaaaaattc gtatccgttt
    41461 aaaagcgtat gaacaccgta ttttagatca atcagcggat aaaatcgtgg aaactgcaaa
    41521 aagaactgga gctagcgtgt ctggtccaat tccattacca actgaacgca gtctttacac
    41581 aatcatccgt gcgactcata aatacaaaga ttcacgcgaa caattcgaaa tgcgtacaca
    41641 caaacgtctt atcgacattg tgaacccaac accaaaaact gttgatgctt tgatgaagct
    41701 agacttacca tctggtgtaa acatcgaaat caaattataa aactaaaaca aaaataatgg
    41761 aggtgtactc atgaccaaag gaatcttagg gaaaaaagtg ggaatgacac aaatcttcac
    41821 tgaatctggt gaattaattc cagtaactgt tgtagaagct actccaaacg tagtactaca
    41881 agtgaaaaca atggaaacgg atggctacga agctattcaa gttggttacc aagatatgcg
    41941 tgaagtttta tcaaacaaac ctgcgaaagg tcatgttgca aaagcaaaca cggctcctaa
    42001 gcgcttcatt cgtgaattca aaaatgttga gctaggagaa tatgaagtag gaaaagaaat
    42061 tacagtagat gttttccaag caggagacat tattgatgta acaggtacta cgaaaggtaa
    42121 aggattccaa ggggttatca aacgccacgg acaaagccgc ggaccaatgg ctcacggttc
    42181 tcgttaccat cgtcgtcctg ggtcaatggg tccagttgca cctaaccgtg tatttaaaaa
    42241 caaacgtctt gctggccgta tgggtggcaa ccgcgtaact atccaaaact tggaagttgt
    42301 acgtgtcgat gcagaaagaa atgttatctt gatcaaaggt aatgttcctg gagctaaaaa
    42361 atctttaatc actatcaaat cagctgtgaa agctaaataa ttgtaggaag aaaggaggaa
    42421 ctaaggaatg ccgaatgtag cattattcaa acaagatgga agccaaaacg gtgaaatcac
    42481 acttaacgaa gaaatcttcg gaattgaacc taatgaaagt gttgtttacg atgcaatcgt
    42541 aatgcaacgt gcgtcattaa gacaaggaac acacgcggtt aaaaaccgta gcgctgttcg
    42601 tggcggtgga cgtaaaccat ggcgtcaaaa aggaacaggt cgtgcacgtc aaggatctat
    42661 ccgctcacca caatggcgtg gaggtggcgt agtctttgga ccaacaccac gttcttacag
    42721 ctacaaactt cctaaaaaag ttcgtcgttt agcaatgaaa tctgtattgt cagaaaaagt
    42781 tgctgaaaac aacttggtag caatcgaagg cttgaacttc gacgcaccaa aaacaaaaga
    42841 attcaaacaa gttcttgcta acttatctat cgactctaaa gtattggttg ttctagaaaa
    42901 aggtaacgac tttgctgctt tatcagcacg taacttacca aacgtttctg tagtaacttc
    42961 tgataatgtg agtgtgctag acgttgtatc aaatacaaaa gtgttagcaa cacaaactgc
    43021 tcttactcaa attgaggagg tgcttgcata atgaacttac tagacgtaat caaacgccca
    43081 gtgatcactg aaaaatcaat gcttgccatg gatgacaaga aatacacttt tgaagtggac
    43141 actcgcgcaa acaaaacttt agtaaaacaa gcggtagaag ctgcttttga cgttaaagtc
    43201 aaaaacgtga acatcgtgaa cgtacgtgcg aaattcaaac gtatgggcaa atatgcagga
    43261 tatacaaaaa aacgtcgcaa agcgatcgtt acattgactg aagattcaaa agaaatccaa
    43321 ttattcgaag ctgctgaata agcactaata gataactcat taaattagga gggaaaaacg
    43381 tggcgattaa aaagtacaaa cctacctcaa atggtcgtcg taacatgaca ggttctgatt
    43441 ttgctgaaat cacaacaaca acacctgaaa aatcattgtt acaaccattg aaaaaccatg
    43501 ctggacgtaa caacaacggc cgcatcactg tacgtcatca aggtggcgga cacaaacgcc
    43561 aatatcgcgt gatcgacttc aaacgtaaca aagataacgt tgttgcaact gttcaaacga
    43621 tcgaatatga tccaaaccgt tcagctaaca tcgcgttagt acactatgaa gatggagtga
    43681 aagcttacat cttagcacct aaaggactag aagtgggtat gaaacttgtt tcaggtccag
    43741 aagcagatat caaagtcgga aatgctcttc cattagaaaa cattccagtt ggtactgtta
    43801 tccacaacat tgaaatgaaa cctggtaaag gtggacaatt gatccgttct gctggtacaa
    43861 gtgctcaagt acttggtaaa gaaggcaaat acgtattgat ccgcttgaac tctggtgaag
    43921 ttcgtatgat cttagctgca tgccgtgcta caatcggttc tgtaggtaac gaacaacacg
    43981 aattaatcaa catcggtaaa gctggacgct ctcgttggat gcgtaaacgc ccaactgtac
    44041 gtggtagcgt aatgaaccct aatgatcacc cacacggtgg tggtgaaggt aaagctccta
    44101 tcggacgtaa agcaccagtt tctccttggg gtcaaccagc tcttggttac aaaactcgta
    44161 acaaaaaagc taaatcagac aaacttattg ttcgtcgtcg caaaaataaa taattacttt
    44221 gaccatgtgt cgcacatttt gagaggaggt tcaccatggg tcgtagttta aagaaaggac
    44281 cttttgtcga tgagcattta atgaaaaaag ttgaagctca agcaggggtt gaaaagaaaa
    44341 aagttatcaa aacttggtca cgccgttcaa cagttttccc aagttttgtt ggatatacca
    44401 tcgcagttta tgatggacgt aaacatgtac cagtttacat ccaagaagac atggtaggac
    44461 acaaattagg tgaatttgca cctacaagaa cttatcgtgg acatgctgct gacgataaga
    44521 aaacgaaacg ctaatttgag gggaggataa atccaaatgg cagaacaaat cacatcagct
    44581 aaagcaactg caaaaacagt tcgcacttca cctcgaaaag cccgtttagt aattgacctt
    44641 atcagaggta aaagtgtcgc agatgcaatt tcaatcttga aattcacacc aaacaaatct
    44701 gctggaatca ttgaaaaagt attgatgtca gcagttgcga atgcagaaaa taactttgac
    44761 ttagatgttg aaaacttggt agtaagcgaa gcttttgtta acgaaggacc tacaatgaaa
    44821 cgtttccgtc ctcgtgcaaa aggatcagct tctccaatca acaaacgtac aagtcatatc
    44881 acggtagtcg tatcagaaaa ataaggaggg ataatcagtg ggtcaaaaag tacatccaat
    44941 tggaatgcgt gtaggcatca tccgcgactg ggatgctaaa tggtatgctg aaaaagagta
    45001 tgctgagttc ttacacgaag atttaagaat ccgtaaattt atttcaacta aacttgctga
    45061 tgctgctgta tcaacagttg aaatcgaacg cgcaacaggt cgcgtaaaca tttcaattca
    45121 cacagctaaa ccaggtatgg ttattggtaa aggcggttct gaagtcgaaa gcctaagaaa
    45181 agaattaaac aaattaactg gtaaaagaat ccatatcaat atcgtggaaa tcaaaaaacc
    45241 agatctagat gcaaaattag taggtgaagg aatcgcacgt caattagaaa accgtgttgc
    45301 tttccgtcgc gctcaaaaac aagccatcca acgctcaatg cgtgctggcg ctcaagggat
    45361 caaaactcaa gtatctggtc gtctaaacgg agcagatatc gctcgttcag aaggatactc
    45421 agaaggaact gttcctcttc atactttacg tgcggacatt gattacgcat gggaagaagc
    45481 agatacaaca tacggaaaac taggagttaa agtgtggatc tatcgtggag aaattcttcc
    45541 aactaaaaaa aacactgaga aaggagggaa ataatcatgt tagtacctaa acgtgtaaaa
    45601 caccgtcgtg aattccgcgg aaaaatgcgc ggggaagcta aaggcggaaa agaagtagca
    45661 ttcggtgaat acggtttgca agctgttgat tcacattgga tcacaaaccg ccaaatcgaa
    45721 gctgctcgta tcgcaatgac tcgttacatg aaacgtggtg ggaaagtatg gattaaaatt
    45781 ttccctcaca aatcttatac tgccaaagca attggggtac gtatgggttc tggtaaaggg
    45841 gcacctgaag gatgggttgc accagtaaaa cgtggtaaaa tcatgtttga aatcgcaggc
    45901 gttcctgaag aagtagctcg tgaagcgtta cgtctagctt ctcacaaatt accaatgaaa
    45961 actaagatcg taaaacgtga ggaaatgggt ggtgaatcga atgaaggtta aagaaatcag
    46021 agaattaacc actgccgaaa tgcttgatca agaaaaacaa ttaaaagaag aattgtttaa
    46081 tcttagattc caattagcaa caggtcaatt agaaaacact gcacgtatta aagaagtacg
    46141 taaatcgatt gcacgcatca aaactgtgtt gcgtgaacaa gctaagtaat tatggaagga
    46201 ggccattaag cgtatgactg aaggaagaaa tcaacgcaaa gtttatcaag gtcgcgtggt
    46261 ttcagacaaa atggataaaa caattaccgt tgctgtcgaa acaaagaaaa accaccctat
    46321 ctatggcaaa cgcatgaact attctaagaa atacaaagtg catgatgaaa acaacacagc
    46381 aaaagttggc gacatcgtga gaattatgga aactcgtcca ttatcagcta caaaacgttt
    46441 ccgtttgttg gaagtagtcg aagaagcagt tattatctaa taacacgaga ttttcctata
    46501 tagattgaat gcaaaatctg aaaggaggat acacaacgtg atccaagcag aaagtcgttt
    46561 aaaagtcgct gataactcag gcgcacgtga aattttgacg atcaaagtcc ttggtggatc
    46621 tggtcgtaaa actgctaaca tcggtgatgt gatcgttgct acggttaaac aagcaacgcc
    46681 aggtggggtt gtcaaaaaag gtgacgtcgt taaagccgtt atcgttcgaa caaaatcagg
    46741 agctcgtcgt acagacggtt catatattaa atttgatgaa aatgctgcag tgattattcg
    46801 tgatgataaa agccctcgtg gaacacgtat ctttgggcca gttgcacgtg aactacgtga
    46861 aaacaacttc atgaagatcg tttcactagc accagaagtt ttataatctt gagacactta
    46921 tcaaaggagg tgcgaaacag ttatgtttgt taaaaaaggc gataaagtta aagttatcac
    46981 tggaaaagac aaaaacaaag aaggcgttgt actagcagcg ttcccgaaaa aagataaagt
    47041 tattgtcgaa ggtgtaaatg tcgttaaaaa acaccaaaaa ccttctcaag ctgctcctca
    47101 aggtggaatt gttgagatgg aagcgccaat tcatgtttct aacgtgatgg tggttgactc
    47161 tactggtgta gctggccgag ttggctacaa agaagtagac ggcaaaaaag tccgtgtttc
    47221 taaaaaaacc ggtgaagttt tagataaata aaagtttaag gaaggagggt cttactgaat
    47281 gaaccgccta aaagaaaaat atcttaaaga agtaactcca tcattgatgg aaaaatttaa
    47341 ctatagctct gttatgcaaa cacctaaagt tgaaaaaatc gtcatcaaca tgggtgttgg
    47401 tgatgcggta tcaaacgcta aaaacttaga taaagcagtt gaagaattaa ctttgatcac
    47461 tggtcaaaaa ccaatgatta caaaagctaa aaaatcaatc gctggattcc gtttacgtga
    47521 aggaatgcca attggtgcaa aagttacatt gcgcggagaa agaatgtacg aattcttaga
    47581 caaattggtt tctgtttcac taccacgtgt acgtgacttc catggtgtaa gtaaaaaagc
    47641 ctttgacggt cgtggaaact atactttagg tattaaagaa caattgatct tcccagaagt
    47701 agattatgat ttagtagata aagtacgtgg tatggacatc gttattgtaa caacagcaaa
    47761 ctctgatgaa gaatctcgtg agttgttgac acaattaggc atgccattcc aaaaataatt
    47821 aaaggaggcg aactacgtgg ctaaaaaatc aatgattgct aaaaacaaac gtcctgcaaa
    47881 attttcaaca caagcttata ctcgttgcga acgttgcgga cgtccacatt cagtttatcg
    47941 taagttccat ctttgccgta tttgcttccg cgaacttgcc tataaaggtc aaattcccgg
    48001 cgtgaagaaa gctagctggt aaaaagtaaa ttcgcataag aaggaggtaa aagatcaatg
    48061 gtcatgacag atccaattgc agattttcta actcgtatcc gtaacgcaaa catggttaaa
    48121 catgagtctt tagaagtacc tgcatcaaaa attaaacgtg acatcgctga aatcttgaaa
    48181 cgtgaaggtt tcgttcgtga tgttgaatac atcgaagatg acaaacaagg cgtgatccgt
    48241 gttttcctaa aatttggtaa aaacggcgaa cgtgttatca caaacttaaa acgtatctct
    48301 aaaccaggtt tgcgtgctta tgtcaaagct gacgaagtac caaaagtatt gaacggttta
    48361 ggtatcgcaa tcatttcgac ttctgaaggt gttgtcactg ataaagaagc tcgtgaaaaa
    48421 aatatcggcg gcgaagttat cgcttacgta tggtaatcaa gtagaatcta caaggaggtg
    48481 tctttaagtg agccgtatcg gaaataaagt cgttgttatc cctgaaggag taacagttac
    48541 tcaagacgga aacaacgtta cagttaaagg accaaaaggt gaattgactc gtactttttc
    48601 agcagatatc aaaatgaata tcgaaggaaa cgaagtaact ttcactcgtc caaacgacag
    48661 taaagaaatg aaaacaattc atggaacaac tcgtgcgaac ttcaacaaca tggttgtagg
    48721 tgtcagcgaa ggtttccaaa aagcgttaga actaatcggg gttgggtacc gtgcacaatt
    48781 acaaggatca aaacttgtat tgaacgttgg ttactcacat ccagtagaaa tcacaccacc
    48841 agctggagta actgttgaag taccagctaa cacacaagta attgttaaag gtgcgaacaa
    48901 agaagaagtt ggcgaattag ctgcaaacat tcgtggaact cgtcctccag aaccttataa
    48961 aggtaaaggt attcgttatg ttggcgaata tgtacgccgt aaagaaggta aaactggtaa
    49021 ataatcgttt agaaaaattg catagaagcc agcagcagga aaaaggcatt tggctgaagt
    49081 ttaccttcaa aaaaatacat ttttacttgt gccgcagctt ctatttttcg aaacgtattt
    49141 actgcttgaa atagtagtac aataaaaata aagaggtgac aattgtgatt acaaaaccag
    49201 ataagaacaa aacacgtcaa aagagacatc gtcgtgtacg taacaatatc tctggtactg
    49261 ctgagcgccc acgcttgaac gtttttcgtt ctaacaaaaa catctacgct caaattattg
    49321 atgacgtagc gggtgtaacg ctagcaagtg cctctacctt ggataaagaa atttcaggtg
    49381 gaacaaaaac agaagcagca caagctgtcg gtaaactagt tgctgaacgt gcagctgaaa
    49441 aaggcattaa agtagtagtc tttgaccgtg gtggatacct ttaccatggc cgtgtgcaag
    49501 ctttagctga agctgctcgc gaaaatggac tagaatttta gaagaaggag gaataccatt
    49561 catggtttat atcgatccaa aacatttgga attagaagac cgcgttgttg caattaaccg
    49621 tgtaacaaaa gttgttaaag gtggacgtcg tctacgtttt gctgctttag ttgttgtcgg
    49681 tgacaaaaac ggacacgtag ggtttggtac aggtaaagct caagaagttc ctgaagcaat
    49741 ccgtaaagca attgaagatg cgaagaaaaa cttagttgaa gtacctatgg ttggttcaac
    49801 aatcccacac gaagtaatcg gtgcttttag tggtggtaga atcctaatga aacctgctgt
    49861 agaaggttct ggggttgctg ctggtggacc agttcgtgcc gtattagaat tagctggggt
    49921 agcagatatc acttctaaat ctttaggctc aaatacacca atcaacgttg ttcgcgcaac
    49981 agttgaaggt ttgaaacaat tgaaacgtgc agaagaagtt gcagaacttc gcggcaaatc
    50041 agttgaagaa ttaatcggat aaggaggaca acaataatgg ctgaattaaa agttacttta
    50101 aaacgcagta ttatcggacg tcctcaaaac caacgcgata cagttaaagc gttaggtctt
    50161 acaaagatta acagttcagt ggttaagcct tctaatgaag caattaaagg catgatcaat
    50221 actgtttctc acctagtgga cgttgaagaa atttaattta gaaaaatcat aactagaaca
    50281 ttaaggaggt gccaaaccta tgaaacttca tgaattaaaa cctgctgaag gttcacgcca
    50341 agtacgtaac cgtgttggtc gtggtacttc atctggtaac ggaaaaactg ccggacgcgg
    50401 acaaaaagga caaaaagctc gttcaggcgg cggtgtgcgt ttaggatttg aagggggaca
    50461 aacaccattg ttccgtcgtt taccaaaacg aggattcaca aacgtgaacc gtaaagacta
    50521 tgcagtcatt aatcttgatg tattgaaccg ctttgaagac ggagctgaag taactcctgt
    50581 tgctttagtt gaagctggaa tcgtgaaaaa cgaaaaagct ggaatcaaag tattagctaa
    50641 tggggaacta aacaagaaat taactgtgaa agcagcaaaa ttctctaaag cagctcaaga
    50701 agcaattgaa gcggctggtg gatcaatcga ggtgatctaa tgtttagact attaaaagat
    50761 gcttttaaag taaaagacat tagatcgaaa atcctattta ctgtatttgt gttgtttgta
    50821 tttcgcctag gtgcacatat tactgtacca ggcgtaaatg ctgcttcatt gcaaaacctt
    50881 tcttctttgc catttttcaa catgttgaac ttggtaagtg gtagtgcgat gcaaaacttt
    50941 tccattttct caatgggggt ttcaccatac attactgctt ctatcgtcat tcagcttctt
    51001 caaatggaca tcgtgcctcg atttgttgaa tggtcgaaac aaggggaagt tggtcgtaaa
    51061 aaactgaatc aagcgactcg atatctgaca ttagtattgg catttatcca atctgtcggt
    51121 atcactgctg gattcaatca atacgctcaa ttaggtttcg tgaataatcc taacgtagca
    51181 acttacgtaa cgatcggttt gattctaaca gccggtacga tgtttgttac ttggctagga
    51241 gaacagatca cagaaaaagg tatcggaaat ggcgtatcaa tgatcatttt tgcaggtatc
    51301 atttctagat tgcctgaaag tatcagagaa atttatgaag attactttat aaatattgat
    51361 ccttcagaca tatggaaatc tgctattttt gtggccattt tactagtagc ggtcttgttg
    51421 atcgtgacat tcgtaacatt tttccaacaa gctgaacgca agatcccgat ccaatatacg
    51481 aaacgcgtag ctggcgcgcc tacaagcagc tatctgccgt tgaaagtaaa tgcagctgga
    51541 gttattccag ttatctttgc tagttcgttt attgctacgc caaatgctgt tttacaagca
    51601 ttgagcggta gattcggttc tgaaaactgg tacaaagtga tgatggagat ctttaactat
    51661 aatacagcac ctggtgctac gatttatacg attctgatcg ttgccttcac tttcttctac
    51721 gcctttgttc aagtaaaccc tgagaaatta gcggaaaact tgcaaaagca aggaagttat
    51781 ataccaagcg tacgccctgg taaggggact gaagagtatg tctcaagatt actgatgcgt
    51841 ttaagtacag ttggtgcagt cttccttggt ttagtggcat tgcttccaat cattgctcaa
    51901 atggtttggg atcttccaca atcaatcgga ttaggcggta caagcttact gatcgttatc
    51961 ggtgtagctt tagaaactgc gaaacagtta gaaggcttaa tgctgaaacg taaatatgtt
    52021 ggatttattg agtaagaaag cgtgttgagg tttcccctca acacacgttc tcacacattt
    52081 attaggaggg aaaacaatga acctcatttt aatggggctg ccaggcgcag gtaaaggaac
    52141 acaagctgaa aaaatcattg atgcttatgg tattccgcac atctcaacag gagatatgtt
    52201 ccgcgcagcg atgcaaaacg aaacagctct tggcctggaa gcaaagtctt atatggataa
    52261 aggtgcatta gttcctgatg aagtaacaaa tggaatcgtg aaagagcgac tagctgaacc
    52321 agatacagag aaaggcttct tattagatgg ctttccacgt acgctagatc aagcaaaagc
    52381 tctggacgca atgctaaaag atttgaataa aaaaattgat gctgtcatcg atatccatgt
    52441 tggtgaagaa atcttggtcg aacgtctagc cggccgattt atctgcagca attgtggagc
    52501 aacttaccat aaaattttca accctacaaa agttgaagac acatgtgatc gttgcggtgg
    52561 gcatgaattc tatcaaagag aagatgataa acctgagacg gttaaaaatc gtctggctat
    52621 caacatcaaa aatagtgaac caatcttggc ttattataaa gaacaaggtt tgctaaacac
    52681 tattgatggt gctcgtgaaa tcgatgctgt atttgcagat gtacaaaaaa tcattgaaaa
    52741 atagcacgat tgcccgtagc cgacttgcat tatgcagtta ctcttgtcaa gttcaataaa
    52801 ttatgataga atatgtcagt ccgacttcta gagaatcctc tagatgatcc attttgagaa
    52861 ttttgaggtg ggaactgcag tttccgccat tttatcgtgt gaaaatgcga aacaagtaca
    52921 aggaggtact gtgcgtggca aaagaagata tgattgaagt cgaaggtaca gtcgtcgaaa
    52981 ctttgccgaa tgcaatgttt aaagttgaac tagaaaacgg acaccaagtt cttgctactg
    53041 tttcaggtaa aattcgtatg cattacatcc gtatcttacc tggtgacaaa gtaacggttg
    53101 aattgtctcc atacgatctt aaccgtggcc gtattactta tcgctttaaa taattgtact
    53161 ccgtaaaact acaggaggta ttatcatgaa agtaagacca tcagtaaaac caatgtgcga
    53221 acattgtaaa gtaattcgtc gtaaaggacg cgttatggtg atttgcccag caaatccaaa
    53281 acataaacaa cgtcaaggat aattggaggt gctataaata tggctcgtat tgcaggagta
    53341 gatattcctc gtgataaacg tgtagtagtt tcacttactt atatctatgg tattggtaac
    53401 actactgcta aaaagatctt agctgatgct ggtgtatctg aagatgttcg cgttcgtgaa
    53461 ttaacgaatg aacaaacaga tgctatccgt gcagaagtag ataaattaaa agttgaaggg
    53521 gatcttcgtc gtgaagtgaa cttgaacatc aaacgtttga tggaaatcgg ttcttaccgt
    53581 ggaatccgtc atcgccgtgg attgcctgtt cgtggacaaa acacgaaaaa caatgcacgt
    53641 actcgtaaag gcccagctcg cgctatcgct ggcaagaaaa aataattctt aagtgaagga
    53701 ggttaaaact tcatggcagc aaaaaaagtg aatcgtaaac gccgtgtgaa gaagaatata
    53761 gaagcaggta ttgcacatat ccattctaca ttcaataaca ctatcatcat gatcactgat
    53821 actcacggaa atgcattagc gtggtcatca gctggttcat taggtttcaa aggaagcaaa
    53881 aaatcaactc cttttgccgc tcaaatggca gcagaaactg ctacaaaagc agcaatggaa
    53941 cacggattaa agactgttga agtaacagtt aaaggacctg gttctggacg tgaagcagca
    54001 attcgttcat tacaagctac aggtttagaa gtgactgcaa ttcgtgacgt gactccagtt
    54061 cctcataatg gtgcccgccc tccaaaacgc cgtcgtgttt aatgagtgcc gctcattttg
    54121 agttttatca gtaacgtcat tgaacaagac acttttcgtt ttgaaagggg taaagataag
    54181 aatgattgag ttcgaaaaac caagaatcgc aaaaattgat gaagaaaaag attatggcaa
    54241 gttcatcgtt gaacctcttg aaagagggta tgggactact ttagggaatt ccctacgtcg
    54301 tattttatta tcttctttgc caggtgctgc catcactaat attcaaatcg atggtgtact
    54361 gcatgaattc tctaccatca aaggtgttag agaagacgtc actcaaatca tcttgaacat
    54421 taaaggctta gcgctgaaaa tgtatggcca agaagaaaaa acccttgaaa tcgatattac
    54481 cggacctgct acagtaactg ctggcgatat tatcgttgat agtgatgtag aaattcttaa
    54541 caaagatatg tacatctgta gtgtctctga aggagctacg ttccatgctc gcttaacagt
    54601 gaaacctgga cgtggttatg ttcaagcaga tgaaaataaa aaagaagata tgccaattgg
    54661 cgttcttcca gtcgattcaa tctacacacc tgttcgtcgt gtgaactatc aagttgagaa
    54721 cacacgtgtt ggacaccgtg aagattttga taaattgacg atggagattt ggacagatgg
    54781 ttcaattgaa ccgttggaag caatgagttt gtctgcgaaa atcatgactg aacatttgga
    54841 tatttttgtg aacttaactg atgaagcaaa aaatgctgaa atcatggtcg aaaaagaaga
    54901 aacacaaaaa gaaaaaatgt tggaaatgac gatcgaagag ttagacttgt ctgttcgctc
    54961 ttacaattgt ttgaaacgtg caggaatcaa tactgtacaa gaacttacaa ataaatctga
    55021 gcctgaaatg atcaaagtcc gtaacttagg acgtaaatca ttagaagaag ttaaagcaaa
    55081 attgcacgac ttaggtttag gtttacgtaa agaagattaa aaccttttac gaaggaggga
    55141 aaccaacgtg agttatcgta aactaggacg cacatctagc caacgtaaag cgatgttgcg
    55201 tgatttaaca actgatttat taatcaacga acgtattgtg actactgaag ctcgtgcgaa
    55261 agaagttcgc tcaactgcag aaaaaatgat cactttaggt aaacgcggag atttgcatgc
    55321 acgtcgtcaa gctgcggctt tcgtacgtaa tgaagttgcc agcgtgcgtg aagaaaatga
    55381 ggacatcgtc attgaatcag ctttacaaaa actgttcaac gatattgcac ctcgttacgc
    55441 agaacgtcaa ggcggataca cacgcatttt gaagacagaa ccaagacgcg gtgatggtgc
    55501 accaatggtt gtcttagaac ttgtctaaaa tcacactaat aacatcactt tatagatgat
    55561 tcttttagag tgttatgatg ttggaagata tttttccgag tctagctcag cgctgctcct
    55621 caagcgaata tcgcttgggg agtaagcact catcataaag tgttttttta taccttcaat
    55681 tacctgtcat gacagatatg tttgaaggca taaaaaaaca ttttaagcga gaaataattg
    55741 aaaattttgt ctttttttga tagtgttagt aggaacttta ggatgaagaa ggagtaatca
    55801 tggagccaat tattgaatta gaaaagataa attataaata ccagccagat gatcttcgcc
    55861 ccgcattgaa agatgtctcc tttacaattg ataaaggtga gtggatcgct attattggac
    55921 acaatggttc aggaaaatcg acacttgcca aaacgatcaa tggattgctt ctgccagaat
    55981 cagggatcgt gaaagtggga aatcaaatat tggatgaaga aaatatttgg acgatccgac
    56041 agatggttgg aatggttttt caaaatccag acaatcagtt tgttggttcg acagtagaag
    56101 atgacgttgc ttttggtctt gaaaatcaag ggattcccag agaagaaatg ctagtgcgtg
    56161 tgaaagatgc acttgagaaa gttagaatgg ctgagtttgc ttcacgcgag cctgctcgtt
    56221 tatcaggagg gcaaaaacaa cgtgttgcca ttgccggcgt ggttgcctta cgacctgata
    56281 tcattatttt agatgaagca accagtatgc tagatccaga aggtcgtgaa gaagtgatct
    56341 ctacgatcaa gaagatcaaa gaagaaagtc aattgaccgt tatatctatc actcatgata
    56401 ttgatgaagc agctaatgcc aatcggatac tagtgatgag acaaggcgaa cttgtccgtg
    56461 aaggtacacc aaaagaaatt ttttctgctg gtcctgaact gatcgactta ggtctagatc
    56521 taccatttcc tgaaaaactg aagagcgcac tgaaagaacg tggggttgat gtgcccagtg
    56581 aatatatgac agaagaaagg atggtggatt ggttatggac atccgttttg aacaagtaga
    56641 cttcacttat cagccgaata caccatttga acaaagagcc ttatttgaca tcaatatgac
    56701 gatcaaagaa aacagttata ctgcattagt agggcatacc ggaagcggga aatcaacctt
    56761 acttcagcat ttgaatgcgc ttgttaagcc aacaagcggt acagtccata ttggagaacg
    56821 agatattcag ccggatacgg ataataagaa tttaaagccc atccgaaaaa aagttggcat
    56881 cgtctttcag tttccagaag cacagctatt cgaagaaacg gtagcaaaag acattgcttt
    56941 cggtcctaaa aactttggtg tcagcgaaga agaagcacta gtcctagcaa aagaaacatt
    57001 agaacaagtt gggctggatg aaagctattt ggaacgttcg ccatttgaac tttcaggagg
    57061 gcaaatgcgt agagtagcaa tcgctggtgt gcttgccatg aggccggaag tacttgtatt
    57121 ggatgaacca acggcaggac ttgatcccca gggcagaaaa gaaatgatgg agatgttttg
    57181 gcgtcttcat aaggaacagc agatcactat tgtattagtt acgcatttaa tggatgatgt
    57241 agccaatttt gcagattatg tctatgtgtt ggaaaaggga cgaatcgtta atagcgggga
    57301 gccgcaagag gtctttcaaa acattgaatg gctaaaagaa aaacaactag gcgtacctac
    57361 tgccaccgag tttgcagaag aattaatggc taaagggatg aactttgcta ccttgccttt
    57421 gacagctgaa gaattagcag acgctatcgt ccattatgct ggaggaaatg cccatgatga
    57481 ataaattgat atttggtcgt tatatgcctg gagattcctt gatccatcga ttggatcccc
    57541 gagctaaatt attagccagt ttttatttta tcggaatcat ttttttagca aataattggg
    57601 aaacgtacct tttgttggca tttttcacat tactttctgt cttcttgtca aaagtagatt
    57661 tgaaattttt tatccgaggc attcgcccat tgatctggct aatcctcttc actgtagctt
    57721 tgcaggtctt gtttacaagt ggcggagaaa cttattggag ttggggtgtt ttccatatca
    57781 ctgaatttgg tgtgatgaac ggcctcttta ttttctgtcg attcgtgttg atcattttta
    57841 tgtcgactct actcacgctt actacaccgc ctttggaatt atcggatgcg attgagtact
    57901 tgcttcgtcc attgaaagcc gtacggttcc cagtccacga aatttcattg atgctgtcga
    57961 ttgctcttcg ttttgtgcct acattgatgg acgaaacaga aaagatcatg aacgctcagc
    58021 gcgctcgcgg tgttgatttt ggtgaaggaa acctagttca gaaaatgaaa gcagtcattc
    58081 ctttgctgat tcctttattt gtcagcagct tcaaccgtgc ggaagatctt gcgactgcta
    58141 tggaagcaag aggctacaaa ggtggggaag gaagaacgaa atatcgtgta ttgcattggc
    58201 ataatagaga cacgatggtt atcattgctt ttgtattatt aactgtgcta ctgatctttt
    58261 taagaggata aacgttcagt gggccgctat ctattctttt cttgctgatc tgtttgcaag
    58321 aaaggatgca agcagtgtat gacaagaaat attagggtct gtggcagact aaggtcacag
    58381 gcctctttct tttatctgaa aagaaagaac gaaaaaattt tatagaaaaa tcattattcc
    58441 agataacctt ttaggaacaa aggagagaaa aacatggtac gttacaaagc aatcattgcg
    58501 tacgacggaa caaattttaa tggattccaa aaacagccaa atgggcgtac tgttcaagaa
    58561 gaagtagaaa aaacattaca aaaaatggca aatggaaaag aaatcacggt ctttggttcg
    58621 ggaaggacag atgccggagt acatgcgatc gggcaagtaa tccattttga ttatccagag
    58681 gagcgtccgt tagaacgaat gcgtttcgcg ctagataccc agtcaccaga ggatattgct
    58741 gtaagacaag tagaaattgt ttcagaagac ttccacgcaa gatatttggt caaagaaaag
    58801 acgtatcaat ttcgagtgga tatagggaaa ccacgcagtc cttttcgacg ccattatgca
    58861 agttattttc cttacccact tgatttggaa aaaattcagc gggcgcttcc tgatttatta
    58921 gggactcacg atttcacctc tttttgtgca tcaggaagct cgatagagga taaaatacgt
    58981 acgatctacg aggctaagat ggaggtaaat gaaacaaaag acgaattgtt gtttacattt
    59041 aggggaaatg gcttcttgta taagatgata cggatcttag taggaacgct tttgaaaatt
    59101 ggcaatggcc gattgcctga agatagtatc ccagatatta ttgcgaaaaa agatcgtaat
    59161 gcagcaggac ctacagcaca ccctgaagga ctttatttgt atgaagtgaa atatgaataa
    59221 gaggttatga tagaagtggt cagcttcaag aaataagaag gtatttacga aaattacttc
    59281 tcaaaatttt gcaaaatttc ggcttatttt cgaagaagct acttctggaa caccgtttaa
    59341 gaaagaggtc gtgagcctgt catttaactc tgagtcacaa agagcaattt caaaaaacag
    59401 cttctcatct ttgatgaata tcgtgacttg tggacgaggc gttgataagt gaatcatctt
    59461 tttgttacct attttttctg taaccaaaaa acgatggaac gttgttttga ttgcccttgg
    59521 tgcggacagt ctgaacaggt tccatcgttt ctttattttc tacgtcttct tctgtgtgtg
    59581 tttcgtggtt gttcttctat tgctggatca atcgtgccat aaaaggggcg attgcggtca
    59641 aggtaccaga cataaagaat aaacaatgca atacatagga caaaaaggat tgcacctatc
    59701 gtaaatcctt caggcaagta actgaaagtg acagtatgtt tacctgctgg aacttttatc
    59761 atcaagaatg cgtctttgaa attgctgacc ttgactggtt tcccatcaat tttagctttc
    59821 catcctttgt cataaggaat agttgtcacc aacatggaat cttcagcggt atttacagtc
    59881 ccagaagctt ttctgccagt ggcttccatt tcgacacctt tttctttgat ctgattcatt
    59941 gcggcttgat aggctacaac atctagacca acgactttag gctccatgaa acttatttct
    60001 ttcgtcccat aaaaacttgc agtaaatgtc actgttgtct cttgaggata atagccaaga
    60061 tcatagtatt gaccagaaat gttgatttgc gtttttcgcg atgtaccatt gacagtgatc
    60121 gtcgcactag aactttctaa ttgcgcaaaa ttggttggga acaaacttaa gtaagcttgt
    60181 gtatttgcag gcacatggac ttcccaagtg agctcttttg gctggttatc tgctgtttct
    60241 gagtaagtga cgccagtcgt tgtctttgtg atcgtcgtat tcaactgatt cttcaaagta
    60301 ggtgtataga atgtaaaata ctgttcattt agaccagaca atgtattgat caagtttgtc
    60361 tgactggtca aattatctgt cactggctgc tcaacatcat taacggacaa cggtgcagta
    60421 aatgctagtg gcaatgcatt gacattctga tacagtgtat agtcgccgct ttgaccagct
    60481 tggcggaagc catatttact gaacgtgctt tcgtttttag aaatgttata tttgattcct
    60541 gtaaacccat ccatcagcaa agtattattg ttataacgga tattcaaatt tgtccctctt
    60601 gaacggaaac ctaatttatc caggtaagag gaagaattac gatttcttat agaagaaaac
    60661 aaactgatac cactgtaacc atagttgatc gaatcgtttg cagatactgg atccagattt
    60721 tccaagcggt aaaacgagtc ggaatctttt tttgtttcat ttacaagagc tttcaagtca
    60781 ggataaggat cagtatataa actgcgggaa gcataattcc aatcatcaag aatgccatgg
    60841 atcatactgt tcgtattgac cagtgcttca gcagacatga tcaataataa caatatactc
    60901 agatgattga atgaaagttt tttatgctga taaaatccca cagcaaaagc ataaatccct
    60961 aagaaaacca ctgtcaatac aaaggaagtc atcgtcacat aggtgtagct tccttcaggt
    61021 ttgatgcccc atgccaccgc aaagagagca gctacgacca aaatgattcc taacaacagc
    61081 ccacgatcgt cttgttctat cttttcccat ccttttcctg caagcaggag cactaagaaa
    61141 gagaacagat aggcataacg gaataaaaac atattcggtg catgcatacc atgccagaac
    61201 aaattcaatg gaacaagata aaaacttgcg ataagtatgg cgaaaagact gccgtacaaa
    61261 aatttatttt tcaacggtac tttccgactg gcaaaataaa acagacagag gactaatggt
    61321 agcagacctg cgtaaacgaa cggaatcgat ccgtattttg tcgtgtcata aacaccaatc
    61381 atatttttca tgattagatc cagataagaa gtcgcttctg ttttcaacgt agtgatctga
    61441 ctcagtgcct cgccatttga ccgaagatcc agcactgctg gcaatacaat gatcattgaa
    61501 gcgccgcctg ccagtaaaga agtgatacca taaggaacaa tcgttttttt atattctgac
    61561 cagtttgtca atagacgggc gataaaatat aagaaagaaa atacgccaat cataaaaccc
    61621 atataaaaac tggataaaaa caagagtaaa taactgataa ataaaagttt cggccgttta
    61681 agatccataa cacgatgaat ccccaaaatc actaatggaa gataaacaaa tgcatccagc
    61741 cacatgatga tctccgaatg tgcagtgata aatgagatca atgcgtaact gacgctaagt
    61801 gcaacatgcc cccagcgagg aatcttgaac gtacgtgcaa gaaaccaaaa agccaatccg
    61861 gcacagccga ccttaagcaa agtcagaaca tacagtgcat ctggcatcat ctggtttgga
    61921 aaaaagaaca ccagtggcgt gaagatcccg cctagatagt aagaaattaa agataaataa
    61981 ttcaatccta gagaagcatt ccatgtataa aaaatacttt gctttccaag aagaacattt
    62041 cggaagctgg catgaaaatt agaaaactgt gaaaaggcat cacttgccaa aatacttctg
    62101 ctgcttccag gatagattcc gatacttaga taaacaagtg ctaaaatcaa aaacggaatc
    62161 aggaaactag ctgctgcgta gcgtccgttt tctctaaaga atgactttaa attttttttc
    62221 atatttattt tccaccaaat ttctatgaag ttgtattcag tttatcatat ccttgttttt
    62281 ctctgcttaa aaagagtcta gttttcacaa aaaaataaaa tttagaataa ataacttcat
    62341 aatttgttcg tataacaaaa ataaacgcaa aaaaagggta ggaatcaaaa gtcccaccct
    62401 taaaaatttt tattaaataa aaaaccgttc actatctgaa cggtccaagt atcattcaat
    62461 taaacgcgtt caacttttcc tgatttcaaa gcacgagttg acacccaaac ttttttaggt
    62521 tttccgtcga ccataacgcg aactttttgt aagttaggtt taacagtacg ttttgttgag
    62581 ttcatagcgt gtgaacggtt gtttccactt tttgtcttgc gacctgtaaa gtaacaaact
    62641 tttgccatgg taatttcctc ctttgccttg atttggagca atagaataat tcagctcata
    62701 ccagactaac atatcatata gaatgggaat ttgcaagtac attttcatag atacaattga
    62761 caattgagaa gaataattgt aaattaaaca aggttatacc tgaaattttg caaggttttt
    62821 gagtactttg ggtaactgcc ttttatttca agttgcgcta tgataaaata tgatagaaga
    62881 aaaatgggag ttttttaccc gttaaggagg ctcattcatg gctgtaaaaa ttcaaacgtc
    62941 agctggaacc attgaaatta ccaatgatgt aattgcaaca gttgttggtg gcgccgcgac
    63001 agatgtgttc ggtgttgtag gaatggccag caaaaatcaa attaaagata atattactga
    63061 gattttgcgt aaagaaaatt actcaagagg agtagttgtc cgccaagaag ataatggtat
    63121 tgctgtagat gtttatacga ttatcagcta tggtacgaaa atctctgaag tttcccgcaa
    63181 cgtccaagaa aaagtgaaat acaatctaga ggcattatta ggcgtaactg ccaattcagt
    63241 taatgtcttt gtacaaggtg ttcgtgtact gccagactag gcaggagact ggctggtaac
    63301 tcagcagtcg atctgaagga ggattatcta ggtgagtgta acagaaataa gtgcaagcca
    63361 gttccaggaa atggtccagg caggtgcgaa tcgtctgcaa gtaaatgcag aatacgtcaa
    63421 ttcattaaat gtattccctg tgccagatgg cgatacggga acaaatatga atctatctat
    63481 gactagtgga gctaaggcag tagtagattc aacctcagaa aaagtaggag aattagcagc
    63541 aatgctttca aaaggattgc tgatgggtgc gcgcggaaat tccggcgtta ttttgtctca
    63601 attgtttcgt ggattttcta agcaaattcc agatgttgtc tcattaaatg cttctgatct
    63661 tgctgcagcc ttcactcatg gagttgaaac tgcgtataaa gcagtaatga aaccagtaga
    63721 aggaacgatt ttgacagtag ctagagaagc agcgaaagct ggagaaaaga aagcaaaaag
    63781 tactgatgat gtcgttgaag taatgacagc tgttgtaaaa ggagcaaagc gtgctttagc
    63841 taaaactcca gacttgcttc cagtcctaaa agaagtcggt gtcgtagaca gtggagggca
    63901 aggactgctg tttgtttatg agggcttttt aagtgcattg aatggagaat tccaagcgga
    63961 tgaaacatat gaaccttctc cagcagaaat ggatgatatg gtcaatgcag aacatcatcg
    64021 cagtgtccaa ggtcaaatgg cgacagaaga tatccatttt ggttactgta cagaaatcat
    64081 ggtaaaaatc ggagaaggac ctacagtcga tagtacattc gattacgaag aattccgtaa
    64141 ctatttagat ggtataggag attctttact tgtggtcaat gatgacgaga tcatcaaagt
    64201 ccacgtgcat acagaaaacc caggagaagt cttgaattat ggacaaaaat tcggttcatt
    64261 agtcaaaatc aaagtcgaca acatgcgctt gcagcatgag acgattttgg aacacgatga
    64321 agaagtgaac gagtttgcga aaaaagaaat accacgtatc ccgtatggta tcgtagcgat
    64381 cgctgctggt aaaggcgtcc aagagctgtt tgaaagttta ggtgctaatt atgttatcag
    64441 tggtggacaa acaatgaatc caagtacgga agacattgtt aaagcgattc aagaagtaaa
    64501 cgcagataaa gtgattgtct tgccaaacaa taagaacatc ttcatggcag ctgatcaagc
    64561 agcagaagta gcagatatcc cagtagcagt tgttccttca agaacgattt cccaaggatt
    64621 gacagctatg ctttcattca atgatcaagc ttctttagaa gaaaacaaag aaacgatgac
    64681 agaaatgttg tctagcgttg tgagcggtca agtcactcac gcgatcagag atacctcaat
    64741 tgacggtgta gagatcaaag aaggagactt tttgggcatg atcgatggaa aaatcctgat
    64801 ctctaaccca gatatcacag caacagcgtt aagtacacta gaacaaatga tcaatgagga
    64861 cacagagatc gtaacgatct tggcaggtga tgaaggaaca gttgaacaag caaatgaatt
    64921 tgctgagaaa ctaacagaaa aacatcctga tttggaaatc gagatccacc aaggagatca
    64981 gcctgtctat ccttatctat tttctgctga ataatttctg tgtcagaata tctttctaac
    65041 acagtattca ttcataaaag gccttcggtt agtgatcagg tatgatcgct gaccaagtcc
    65101 tttttttgtt aaaggaggga ggaaaaattt atgcgttctt tagatgatcc aatcactatg
    65161 ctggcaggag tcggagaaaa aagagcagct agtcttgcca gcttgggcat ccaaacgatt
    65221 gaagacttac tgacttatta tccttttcgc tacgaagata ttcaggaaag aaatctaaac
    65281 gagatccagg atcaagaaaa ggttactttg aaaggaatcg tagtctcgcc tcctgtgatg
    65341 aatcgatttg gttataaaaa aagccgattg caatttcgta tgatgcaaga acatgatgtg
    65401 ttcaacgttt ctttttttaa tcaaccttat ctaaaagaaa aagtcatttt atcggaggaa
    65461 atcgccgtat acggtaaatg ggatgcaaag cgtaaagcct tgaacgggat gaagatttta
    65521 ggttctcaaa caatggatga cttttcgccg atatatcacg tcaacaaatc aattcgtcaa
    65581 acgacattag ttgatctaat caaaaaaggg tttcaagaat acggcacatt gattgaagaa
    65641 aatctgccaa acaaccttgt ggagaaatat cgactgcttg accgagcttc tgctgtaaga
    65701 agtatgcatt ttccaaaaga ccacgaagaa aaccatcaag ccaaacgacg tgtcgttttt
    65761 gaagagttct ttttatttca aatgcgtctt caaggattga aaaaagcaga aaaagcagaa
    65821 acgaatggtt tggagatcct ttatgatgtc caaaaattga aacaatttac tcaaaagcta
    65881 ccttttgaac tgacaaatgc acaaaaacgt gtaacgaatg aaatctgtcg ggatcttcgc
    65941 agtccgcagc atatgcagcg acttttgcag ggagatgtag gtagcggaaa aacagtggtt
    66001 gccgcgattg cgttatacgc taccgttaca gcaggtttcc aaggtgcgtt gatggtgcct
    66061 acagaaatct tggcacaaca acatatggaa agcttgcaac agctgtttga tcccaatgaa
    66121 gtaaccatcg cattgattac ggggtcaacg aaagcaaaag aacgacgtga gctccttgaa
    66181 aaactagagc aaggggaaat taacgtcgtg atcggcacac atgcattgat ccaagatgga
    66241 gtcgaatttc agaatttagg tctagttatt acggatgagc agcaccgttt tggcgtgaac
    66301 cagcggaaag tacttcgtga aaaaggctgg cgaccagatg tcttatttat gacagcgact
    66361 ccgattccta gaacattagc tattacagca tatggtgaga tggatgtatc tatcattgat
    66421 gaacttccag ctggacgtat cccgatcgaa acacgctggg taagaacacc tcaattggac
    66481 agtgtactgg attggacgaa aaaagaactg gcaagaggac atcaaatgta tgtcatctgt
    66541 ccgctgattg aagaatcaga agcattagat gtaaaaaatg cagtggaaat ctatgaaaaa
    66601 cttcgtgaac tatttgcacc aaaatatgaa gttggcttgt tacatggtaa aatgaagaat
    66661 caggaaaaag aagtaatcat ggaagccttc aaagacaacc agatgcagat attagtttcg
    66721 actactgtaa ttgaagtagg ggtaaacgtg ccgaacgcga ccgtcatgct gatcatggat
    66781 gcggatcgat ttggattggc acagctgcac caacttcgtg gacgtgttgg acgcggaagt
    66841 gatgcttcct actgtatctt agttgctaat cctaaaaatg aactaggtgt ggaacggatg
    66901 aaaatcatga cagagaccac caatggtttt gtactaagcg aaaaagatct ggaattacgt
    66961 ggtcctggtg aagtatttgg cttccgtcaa tctggtctgc ctcaatttgc gattgctgac
    67021 ttggtgacgg atggaaatgt tttggaggta gccagagaag aggctgcagc cctttggcaa
    67081 ataaaggatt ggcagatgct tcctgagtat cagcctttag cgcaaagcct ccaaatagaa
    67141 aatcaggata atcgttattt cgattagcag actgtgctat actttatact gatgacggaa
    67201 aaaggagtgg aaacaatgaa aattgccgta gatgcaatgg gcggagatca cgcacctcaa
    67261 gcaattgtag aaggcgtggc tttagcgcag caagattttc cagaagtaga attcttactt
    67321 tacggaaaag aagcagaaat caagaaatac ttaacaaatg aaaaaaacat cacgattatc
    67381 catacagacg aaaaaatcaa tagtgatgat gagccagtaa aagcaattcg acgaaaaaag
    67441 actgcatcaa tggtccttgc tgcacaagca gtgaaaaacg gtgaagcaga tgctgtattt
    67501 tcagcaggga atacaggcgc gctactagca gcagggttgt tcatcgtagg acgtatcaaa
    67561 aatatcgaac gccctggctt gatgtctacc ttgccagtag tagggaaaga aggtgccggt
    67621 ttcgatatgc tggatcttgg cgctaacgca gaaaacaaac cagaacattt gttgcaatac
    67681 ggtattttag gttcttttta tgcacgtaaa gtccgaggta tcggacgtcc tcgtgtcgca
    67741 ctcttaaaca acggaacaga agcaacaaaa ggtagtgaag tgacgaaaaa agcctatgaa
    67801 ctccttcaga atgaaacaag cttgaatttt attggaaacg tagaagctcg tgagctactc
    67861 aatggtgtgg ctgatgtagt ggtcacagat ggatttacag gcaacgctgt tttgaaatct
    67921 attgaaggaa cagcaatgaa tatgatgaat ctattaaaat ctgctattct aaatgaaggg
    67981 atcaaaggaa aaatgggcgc tatgctttta aaagacggct tacgttcttt gaaagcagaa
    68041 atggattatt ccaaacatgg cggtgccgtt ttgtttggct tgaaagcacc agtcattaaa
    68101 actcatggag caacaggccc cgatgctgtt cgctatacga tccgccaaat ccacacaatg
    68161 cttgagacag atgtagttgg acaattagtc gaacaatttg aaaaaagcga agaatagtat
    68221 ttggatatct gaagcgatcc gttccattga aaaaaaggtg gacattcctg aaaaatgcca
    68281 gtaaaattag ccatggtgta ggtaattgac aagtgtatga ttcttggagg tgaaaaagtt
    68341 gactcgcgaa gaagtattta acaaagtagc gaaaattatt tcaaatcatt ttgagataga
    68401 taccgataaa gtgacggatg aattgaacat caaagatgat ttaaaagctg attcaattag
    68461 tattatggaa ttcgttttag agcttgaaga tgaattcggt acggaaattt cagatgaaga
    68521 cgctgaacaa attgaaacag tcggtggcgc agtagattat atcagcagcc atcttaaata
    68581 atttcaaata aaagcaaaga aggataaaaa gctgcatgca gcttttt
//
LOCUS       DAZABD010000008        60168 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000008 DAZABD010000000
VERSION     DAZABD010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 60168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60168
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.008"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(2..1846)
                     /locus_tag="ACHALG_000564"
     CDS             complement(2..1846)
                     /locus_tag="ACHALG_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2586004.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
     gene            2058..2576
                     /locus_tag="ACHALG_000565"
     CDS             2058..2576
                     /locus_tag="ACHALG_000565"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFN2586005.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            2704..3387
                     /locus_tag="ACHALG_000566"
     CDS             2704..3387
                     /locus_tag="ACHALG_000566"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HFN2586006.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            3696..3769
                     /locus_tag="ACHALG_000567"
     tRNA            3696..3769
                     /locus_tag="ACHALG_000567"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3730..3732,aa:Arg,seq:tct)
     gene            3928..4287
                     /locus_tag="ACHALG_000568"
     CDS             3928..4287
                     /locus_tag="ACHALG_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFN2586007.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            4410..6041
                     /locus_tag="ACHALG_000569"
     CDS             4410..6041
                     /locus_tag="ACHALG_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HFN2586008.1"
                     /translation="MFKKYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(6103..6744)
                     /locus_tag="ACHALG_000570"
     CDS             complement(6103..6744)
                     /locus_tag="ACHALG_000570"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFN2586009.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    6930..7006
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            7026..7334
                     /gene="rplU"
                     /locus_tag="ACHALG_000571"
     CDS             7026..7334
                     /gene="rplU"
                     /locus_tag="ACHALG_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFN2586010.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            7361..7705
                     /locus_tag="ACHALG_000572"
     CDS             7361..7705
                     /locus_tag="ACHALG_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HFN2586011.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            7718..8011
                     /gene="rpmA"
                     /locus_tag="ACHALG_000573"
     CDS             7718..8011
                     /gene="rpmA"
                     /locus_tag="ACHALG_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFN2586012.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            8445..11252
                     /locus_tag="ACHALG_000574"
     CDS             8445..11252
                     /locus_tag="ACHALG_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFN2586013.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            11443..11934
                     /locus_tag="ACHALG_000575"
     CDS             11443..11934
                     /locus_tag="ACHALG_000575"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFN2586014.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            12037..13026
                     /locus_tag="ACHALG_000576"
     CDS             12037..13026
                     /locus_tag="ACHALG_000576"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFN2586015.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            13059..13862
                     /locus_tag="ACHALG_000577"
     CDS             13059..13862
                     /locus_tag="ACHALG_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFN2586016.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            13883..14797
                     /locus_tag="ACHALG_000578"
     CDS             13883..14797
                     /locus_tag="ACHALG_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFN2586017.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            14880..15254
                     /locus_tag="ACHALG_000579"
     CDS             14880..15254
                     /locus_tag="ACHALG_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HFN2586018.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            15393..15638
                     /locus_tag="ACHALG_000580"
     CDS             15393..15638
                     /locus_tag="ACHALG_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFN2586019.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            15810..16874
                     /locus_tag="ACHALG_000581"
     CDS             15810..16874
                     /locus_tag="ACHALG_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFN2586020.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            17120..17545
                     /locus_tag="ACHALG_000582"
     CDS             17120..17545
                     /locus_tag="ACHALG_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFN2586021.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            17538..17987
                     /gene="nusB"
                     /locus_tag="ACHALG_000583"
     CDS             17538..17987
                     /gene="nusB"
                     /locus_tag="ACHALG_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFN2586022.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            18084..18938
                     /locus_tag="ACHALG_000584"
     CDS             18084..18938
                     /locus_tag="ACHALG_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HFN2586023.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            18939..20276
                     /gene="xseA"
                     /locus_tag="ACHALG_000585"
     CDS             18939..20276
                     /gene="xseA"
                     /locus_tag="ACHALG_000585"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFN2586024.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            20281..20505
                     /locus_tag="ACHALG_000586"
     CDS             20281..20505
                     /locus_tag="ACHALG_000586"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFN2586025.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            20507..21397
                     /locus_tag="ACHALG_000587"
     CDS             20507..21397
                     /locus_tag="ACHALG_000587"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFN2586026.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            21406..22224
                     /locus_tag="ACHALG_000588"
     CDS             21406..22224
                     /locus_tag="ACHALG_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HFN2586027.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            22331..22780
                     /locus_tag="ACHALG_000589"
     CDS             22331..22780
                     /locus_tag="ACHALG_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFN2586028.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            22798..24480
                     /gene="recN"
                     /locus_tag="ACHALG_000590"
     CDS             22798..24480
                     /gene="recN"
                     /locus_tag="ACHALG_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFN2586029.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(24535..24651)
                     /locus_tag="ACHALG_000591"
     CDS             complement(24535..24651)
                     /locus_tag="ACHALG_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HFN2586030.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(24724..25680)
                     /locus_tag="ACHALG_000592"
     CDS             complement(24724..25680)
                     /locus_tag="ACHALG_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFN2586031.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            25851..26219
                     /locus_tag="ACHALG_000593"
     CDS             25851..26219
                     /locus_tag="ACHALG_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HFN2586032.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            26389..26820
                     /gene="mraZ"
                     /locus_tag="ACHALG_000594"
     CDS             26389..26820
                     /gene="mraZ"
                     /locus_tag="ACHALG_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFN2586033.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            26841..27800
                     /gene="rsmH"
                     /locus_tag="ACHALG_000595"
     CDS             26841..27800
                     /gene="rsmH"
                     /locus_tag="ACHALG_000595"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFN2586034.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            27805..28206
                     /gene="ftsL"
                     /locus_tag="ACHALG_000596"
     CDS             27805..28206
                     /gene="ftsL"
                     /locus_tag="ACHALG_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFN2586035.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRRVK"
     gene            28207..30399
                     /locus_tag="ACHALG_000597"
     CDS             28207..30399
                     /locus_tag="ACHALG_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFN2586036.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKTGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            30425..31387
                     /gene="mraY"
                     /locus_tag="ACHALG_000598"
     CDS             30425..31387
                     /gene="mraY"
                     /locus_tag="ACHALG_000598"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFN2586037.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            31401..32762
                     /gene="murD"
                     /locus_tag="ACHALG_000599"
     CDS             31401..32762
                     /gene="murD"
                     /locus_tag="ACHALG_000599"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFN2586038.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            32788..33876
                     /gene="murG"
                     /locus_tag="ACHALG_000600"
     CDS             32788..33876
                     /gene="murG"
                     /locus_tag="ACHALG_000600"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFN2586039.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            33891..35111
                     /locus_tag="ACHALG_000601"
     CDS             33891..35111
                     /locus_tag="ACHALG_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFN2586040.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            35267..36592
                     /gene="ftsA"
                     /locus_tag="ACHALG_000602"
     CDS             35267..36592
                     /gene="ftsA"
                     /locus_tag="ACHALG_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFN2586041.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            36615..37856
                     /gene="ftsZ"
                     /locus_tag="ACHALG_000603"
     CDS             36615..37856
                     /gene="ftsZ"
                     /locus_tag="ACHALG_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFN2586042.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            37873..38550
                     /locus_tag="ACHALG_000604"
     CDS             37873..38550
                     /locus_tag="ACHALG_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFN2586043.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            38564..39163
                     /locus_tag="ACHALG_000605"
     CDS             38564..39163
                     /locus_tag="ACHALG_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HFN2586044.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            39244..39465
                     /locus_tag="ACHALG_000606"
     CDS             39244..39465
                     /locus_tag="ACHALG_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HFN2586045.1"
                     /translation="MVYALLSWFPGGYQSGLGRLLAKICEPYISLFDRLPLRIGPLDF
                     TILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            39514..40296
                     /locus_tag="ACHALG_000607"
     CDS             39514..40296
                     /locus_tag="ACHALG_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFN2586046.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            40317..41024
                     /locus_tag="ACHALG_000608"
     CDS             40317..41024
                     /locus_tag="ACHALG_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HFN2586047.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            41286..44084
                     /gene="ileS"
                     /locus_tag="ACHALG_000609"
     CDS             41286..44084
                     /gene="ileS"
                     /locus_tag="ACHALG_000609"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFN2586048.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKSYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(44133..45659)
                     /gene="zwf"
                     /locus_tag="ACHALG_000610"
     CDS             complement(44133..45659)
                     /gene="zwf"
                     /locus_tag="ACHALG_000610"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFN2586049.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(45674..46321)
                     /locus_tag="ACHALG_000611"
     CDS             complement(45674..46321)
                     /locus_tag="ACHALG_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFN2586050.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(46505..46834)
                     /locus_tag="ACHALG_000612"
     CDS             complement(46505..46834)
                     /locus_tag="ACHALG_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HFN2586051.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            47011..47739
                     /gene="sfsA"
                     /locus_tag="ACHALG_000613"
     CDS             47011..47739
                     /gene="sfsA"
                     /locus_tag="ACHALG_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFN2586052.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            47755..48768
                     /locus_tag="ACHALG_000614"
     CDS             47755..48768
                     /locus_tag="ACHALG_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFN2586053.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            48768..50045
                     /locus_tag="ACHALG_000615"
     CDS             48768..50045
                     /locus_tag="ACHALG_000615"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFN2586054.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            50108..52810
                     /locus_tag="ACHALG_000616"
     CDS             50108..52810
                     /locus_tag="ACHALG_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFN2586055.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            52962..53279
                     /locus_tag="ACHALG_000617"
     CDS             52962..53279
                     /locus_tag="ACHALG_000617"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFN2586056.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            53309..53629
                     /locus_tag="ACHALG_000618"
     CDS             53309..53629
                     /locus_tag="ACHALG_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFN2586057.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            53737..55197
                     /locus_tag="ACHALG_000619"
     CDS             53737..55197
                     /locus_tag="ACHALG_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFN2586058.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            55265..55486
                     /locus_tag="ACHALG_000620"
     CDS             55265..55486
                     /locus_tag="ACHALG_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2586059.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            55517..55699
                     /locus_tag="ACHALG_000621"
     CDS             55517..55699
                     /locus_tag="ACHALG_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HFN2586060.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            55699..56112
                     /locus_tag="ACHALG_000622"
     CDS             55699..56112
                     /locus_tag="ACHALG_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HFN2586061.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            56235..57416
                     /locus_tag="ACHALG_000623"
     CDS             56235..57416
                     /locus_tag="ACHALG_000623"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN2586062.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(57487..57651)
                     /locus_tag="ACHALG_000624"
     CDS             complement(57487..57651)
                     /locus_tag="ACHALG_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2586063.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            57805..57878
                     /locus_tag="ACHALG_000625"
     tRNA            57805..57878
                     /locus_tag="ACHALG_000625"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:57839..57841,aa:Arg,seq:cct)
     gene            complement(57924..58037)
                     /locus_tag="ACHALG_000626"
                     /pseudo
     CDS             complement(57924..58037)
                     /locus_tag="ACHALG_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(58420..59653)
                     /locus_tag="ACHALG_000627"
                     /pseudo
     CDS             complement(58420..59653)
                     /locus_tag="ACHALG_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            59910..>60168
                     /locus_tag="ACHALG_000628"
     CDS             59910..>60168
                     /locus_tag="ACHALG_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN2586064.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFN"
BASE COUNT        19817 a        10202 c        12845 g        17304 t
ORIGIN      
        1 tttatacagg attcgggtaa tttcgagaga gttgaacagt gatccgatca atgaaagtat
       61 ctttttcctg cgttagtgtt cctgtgtaac gatccgctac gtctttcatc atcattaagt
      121 caaaacgttc ctgatcatct aattcttctg tgatgcctcg actaaatagg tttgtatcgt
      181 aatgtgcact atgttcgcaa caaatgacga aatgttcctc ttgttcgtgt atcgacaaat
      241 aaatttttcg atacagtgga ttttcgatgg catgagtttc tctaaaggaa tgctctaaag
      301 cgtggatcaa aagttgagcc agatcgtcat cgttgatagg aagttcttcg ggaagatctg
      361 cttggaaggt tatctgaata ttccgtttgg cagctaactt ctggtaacgc gctaagataa
      421 gatttgtcac ctggtggttg caaaaagaac acagcatttc gaaatttttt gcttcttcta
      481 atatcttttc cacatagttt ttagcttttg tttggtccgt atgcattaaa tcttgcagat
      541 tctccatctc ttggacaaat tccaggcgaa gtgtatttac ttcttggata tgctgtcgaa
      601 gctgctcata atgttgctcc aacaaatcat ttttgatttt taagaaatct acttgtctcc
      661 gatctttgtc ggtatttaag atgtgatcga ttagattgta gctgataata acgattagaa
      721 tgataatata taaaatcgta gaccaattga tcgttgtttg atagatacct aatgccgttt
      781 ggatgtaaat caagcaatgg ataatggcta ccaagacgat ccaaggtgta caagctacat
      841 aaaaacggtt attcttatat gcttcgccaa tgcatgcaag tgaagtgact aaagtgctta
      901 taacactagc aatattagca atcggcatta attcaggaag ttctgcagga gtaaaagttg
      961 cataaagcag aacgccgata aaaatcaaaa gttggaacgt acaaaaaatt ccgtaccatt
     1021 ttttatagta aaacattcgg ctccaatagt agcagatgat gaaagctgac gttaagataa
     1081 taaataaatg agataaaaaa gaatgaacga atggttcaaa aagaatacca cttaaaatat
     1141 cttctgatac agcttcaaat cctaatgcca aagcaaagca acttaagaga ataagtgagt
     1201 attcaagacg tttttgttta tacatcaagt agatcgtata gaaaaaggtc cctatagcga
     1261 taaaaacagc gattatcatc gtcaatactt gcggcactga ttgtgaataa ataaaactaa
     1321 tcaatggtcc agttgttcct agataaactt tcggtggcag tccagcatag ttctgataag
     1381 gagatgagac tgtaagggtc aatttttttc cgatataatt tgctggtaaa tctatcacag
     1441 ccaatgagag tccgggattg ctttcttcct gataatcttt ccgttggtat aaaatctttt
     1501 cgcctagata tacagtaatc cattgatggt ttcctttgat tagtaaattt gcttcgtcca
     1561 acggtttttc aagtgtcgtt tccataatga atgtttcgtc tttatccacg gtaggaatcg
     1621 tacgagtgta cttgctgtca aacttaaaat caggatcatc ttttgtatag aaaacccacg
     1681 aatcttttat ttctttcatt tgattgctgt ccgcaggaga aaaaataaaa taaaagaaat
     1741 aagcaattcc tgttaaaaat aacaatgata atataatgat tatgctccac tttatacgta
     1801 aataaaaaga ttctttttta ttatatatgt tattaaaaaa agacataaag gtattccttt
     1861 ctaaaacttg taatgttagg gtaaacgttt tttaaactaa cgtcaattat ttataataaa
     1921 aaatgagata aagatgagga aatagaatta gaaacatata attattaaag ggaaacattt
     1981 ttacagctat aatttgaaaa aaatttatcg ttattatatg attaagaagg attgaataaa
     2041 gaaagaagtt gaaaacgata cctgattaca aaaaaataac ttttataagt tctataacgt
     2101 tgattatatt tttaattctt attcttctcg tcgtcttggt tccccgaatt tttggtttga
     2161 ctccttatat tattgaggac aaagaaatga gtccatatta tgcacaagga aatctgatat
     2221 atgtacgacc gaaaaataca aatgagattt tagtcggaga tattattaca tattatgaaa
     2281 attcaggaaa aaaaatctcg acgcgtcgcg taatagccgt tgaaaacaac cacgaagatt
     2341 tttatacaaa agctgataca aagacttata ttgagcctgg agtagtaaaa aagagaaata
     2401 ttatcggtag tcccatcttt cagattccat atattggact agtcctttca aaaacggcat
     2461 ttgcatggat tcaggggtta ttctttacga ttgctttttg cctaacagga atcacagcat
     2521 ggaatacctt tgtagagttg aaaaagaagc ggagacgaac acagaaattt ttgtaagaat
     2581 gttaaaaaca taaatgtatt ccagagatcc aacgactctt ctgaacatga aacacgagaa
     2641 aacactcatg cagcttgtag atttagtcag ttgttcgtat aatgtgtgaa gaggaggagt
     2701 agtatgcgca atgaaatgat gcatcgtcct gtagtcaaag aagaactatt ggattttatg
     2761 cgtacccagc aaaaaaaatt accgggtgag ctaggcaaac tggaagaaga agctcatgta
     2821 gcagaagtac cagttattcc ccatgaaaca gttgtatttt tgaaattttt acttggacag
     2881 cttcagcctg aacgaatctt ggagatcgga gcagctatcg gtttttcatc aagtgtcatg
     2941 gccacgacaa tcggagaaaa cgctcatgtt acgacaattg atcgtttcga tgtcatgatc
     3001 gagaaagcta aaaaaaacta tgaacgtcta ggactgacag ataaagtgac tttgcttgaa
     3061 ggtcaagcag cagatatctt gccggagttg tctggtccgt atgactttat ttttatggat
     3121 agtgcaaaat caaaatatat tgaattttta cctgaatgct tacgtttgtt aagaaaaggt
     3181 ggcgttttga tggtagatga catcttccaa ggaggaacga tcttgctccc agatgaagag
     3241 attccccgtg gcaaacgagc tatccatcgt aaattaaatg aatttcttcg tgttgtgatg
     3301 gatcatcccg atttgacatc gacaatcttg ccattaggtg atggtgtgat cttaatgaca
     3361 aaggaatcag aaacaatcga gttgtgatac atgattatgg cagcctgtag ataatcgatt
     3421 gaatcgaggg ctcaattcga acttgatgta aaagtttcta tatttgtaag tcaattaaag
     3481 gatacaggaa atctaaataa acaggagtag catgaagtca atgtagctcc tgttttcaat
     3541 tgataacctt agaacagctg aaggagaatg tctcgtttta aaagattgat ttagtagttg
     3601 ggccaaaata attcattatt tgctaattaa ataactattt cgtattgaca gaagcaattt
     3661 aattatctat aattattgat gttccgctga ttcttgtcat agtagctcag ctggatagag
     3721 catccgcctt ctaagcggac ggtcgggggt tcgaatccct cctgtgacgc taaaaaaccg
     3781 ttagaaactg aggtttctaa cggtttttct ttgttttttg tgccaatatt gtttgttttc
     3841 aaaggcaaat aaaataaaaa aacatcctta tcgagtacaa ttataaggag caaaaaaata
     3901 aaactcatca gaaaagagga agaagatatg acgatactat ctagaatttt agtgactcta
     3961 gttgcaattg aattttttta tatcatgtat atcgaaacgg tgagaactga ttctgacacg
     4021 acaagtaggg tttttaaaat gagtaaagaa gagttaagca gaaaatctgt tcaaacattg
     4081 tttaagaacc aaggagtgta caatggacta ttaggagtgg gactacttta tggagcttat
     4141 ctttcttccg catctaaaga aatcacaagt atgctattaa tatctatttt ttttgttgca
     4201 ttatatggca gtttgactag cgatcgttgg attttactca aacaaggtgg gctagctctg
     4261 ttggcattgc tttctatttt cttataaata tagaaaaaat aagaatttat ctaacaggaa
     4321 actttgtgaa aaatattact ttatctcatg aaatatgata ttctaatgaa tggtatttta
     4381 gaaaatttca tgaaaaagga gttagatagg tgttcaaaaa atatgtttcc tcactaaaaa
     4441 aagaattttc aggttatgat ctttcaagat ttcagaaaga tcttttggct gggatcacag
     4501 tagctgctgt agcattgcct ttagccttgg cttttggggt atcaagcgga gctgatgcgg
     4561 ccgcaggttt gattacggct atcattgccg gtatcgttat tggcggttta tcaggcggtt
     4621 tttatcaaat atcaggtccg acaggtgcga tggcagctat cttgatgtct attgcggcaa
     4681 caaagggaat gcaaggtgta ttgcttgcta cttttttagc gggtctattt ttacttgcag
     4741 caggactttt ccgattagga acactgactt cttttatccc agcacccgta attacggggt
     4801 ttacttcagg tattgctatc attattgcat taggtcagat cgataattta tttggtgttc
     4861 actcggaagg tacaaacgtt attgaaaaac taagtagtta tcaaacgata ggatttccga
     4921 taaacacagc ctctttgatc atgggagggc tagtcatttt agggatgttt atctatccaa
     4981 aaaaatgggc gaaacgaatg cctagttcgc tgctggctat cattttggca acagcagtta
     5041 tgctattgtt ccatttacca gtagcaactg tgggtaagat tccacaaaca ttgatcagta
     5101 gtaatcgttt gaatatggga gattttagct tttctgcctt gcaacaagtg gcagttccag
     5161 ctattagcat tgctttatta ggaatgattg aaagcttgct atgtggtgct tcggctggcc
     5221 gaatggcaaa taagccctta gatagcaatc aagaattaat ggcgcaagga attggaaatc
     5281 tgttgcttcc tttctttggc gggattcctg ctactgcggc catcgcccgt acaagcgtgg
     5341 ccatcaaatc tggtgcacaa actagattag ctggtatgat acatgcagtt gtcttactgc
     5401 tctccatgct tgtttttgcg ccagtgatgt cacagattcc aatgccagca cttgcaggtg
     5461 ttttgattgt gacggcttgg cgtatgaatg aatgggaaac gatcaaggag ctatttacta
     5521 ataaatattg gtcagcagtt ctattgttta tccttacgat gatttgtaca gttatctttg
     5581 atttaagtat tgctatcgtg atcggggtga tcggcggctg tgtatttttt gttgtcaaaa
     5641 gcgctgctat cacgatttca ttagaaacga tcgattggca aagaatgggg ctgccagaaa
     5701 cggaaaagct gaataactgg acagtagtgt atattagtgg tccactgttt tttatgtctt
     5761 cagaaaaact caaaaataaa ttggaagaac taaatcatca cgatggtgtc attttctcca
     5821 tgcgaggcgt accaagtatt gatttgacgg cacgtgcagt ttttgatgaa tttcaagaaa
     5881 aagcagagca aagaggacaa acgattatct atacttcctt gcagccagaa gtagaaaaac
     5941 aactttctca cttgtgggaa catacattca cgcatcaaca tcaaacagtt gctcatgcgt
     6001 tgacagcttt acatgcgaaa tatactgcaa ttagccatta aaacaatgaa aaagaacaga
     6061 caagactgta aactgaagat tttatggtct tgtctgttct ttttaatatt gctgtaaaag
     6121 tgaagctagt acttgccagt cttgatggat aacaggtaca agggagcgat tgtagaaaat
     6181 agctgctgga tgaaaaagtg gaagaattcg atagtttttt tctgaccatt cgtaggcatc
     6241 cttttgttct gttaattgta agacaggccc tgtatagagt tgaccatgat ttttggtaac
     6301 cgtatattct ttgccaagta aacgttgaag accaatgttt ccaagtgtcg caattaattt
     6361 tggctggacc ttcatcagtt cgtaatccaa tattggtgca tgcgcaaata cttcggaacg
     6421 agtaggtgtc cgatttggat aaacagtttc tgactgttgt gtccgtttat tgatccgatg
     6481 agtcacgcga tacgggcggc tccttacagc actggtgata tacacctctt ctcgtgttaa
     6541 gccagttgaa gaaagtgcct gcatcaattc ttttccagct tgaccgctaa aaggaacatg
     6601 cgtatcaatc tctgttttcc ctggggcttc tcccacgagc atcagtttag gatgaagagg
     6661 tccttgccca gcgataaatc cttctacagg acggccgttc attcttaaag aagcttcttg
     6721 tatcagttct tttgggtatt ccatttatgc tcctcctttt tcttttatta taagaataaa
     6781 agaaacacaa tgcgactatt ttatttggta gaactgtgag gatagaaaag cagaagtatt
     6841 gcttgaattt ttgttttact ttttgatttt aaatttagtt gactaacggt tgctgttctg
     6901 gtaagctagt actgttgtaa ttgtggactc cacagctaca accgctcaga acaagtaata
     6961 agcacagcat atgctgtcac ttcggatggc gagtctaagt atatgagaag gaggtgcttt
     7021 atagcatgta cgcaattatt aaaactggtg gtaaacaagt aaaagttgaa gtgggacaag
     7081 caatctatgt tgaaaaactt gacgttgaag caggagaaaa agttgttttt gacgaagtta
     7141 tcttagtagg cggagaatct acaaaagtag gcgctccaac tgttgcaggt gcaactgttg
     7201 aaggtactgt tgaaaaacac ggcaaacaaa agaaagtcgt tactttcaaa tacaaaccta
     7261 aaaaacattc acaccgtaaa caaggtcatc gtcaaccata tacaaaagtg gttatcgacg
     7321 cgatcaacgc ttaatagcgt ttgagaaaga aggcctatat atgatcaaag gaacatttaa
     7381 gcgaaatgac gcaggtcaga tcgtttcatt cactttgacc gggcatgctg atgctggtcc
     7441 ttatggaagt gatatcgttt gtgcgggtgt ttctgcgtta gcgatcagca ctgtcaacgg
     7501 gattgcctca ttagccggtt ttgagccgat tgttgaaatg aatgaagaag aaggcggtta
     7561 tctttatacc gaagtcactt caggaatgac acaagaacaa aacaatattg ctgagattct
     7621 attagaaaat cttttattag gattacaatc gatagaagct gaaaactcag aatatatcca
     7681 aattaaaaca attaatgata aataggaggt gcagaccatg ttgttatcta tgaatctaca
     7741 attatttgcc cacaaaaaag gtggcggttc tacatctaac ggccgtgact cagaatcaaa
     7801 acgtctaggt gctaaacgtg ctgatggaca aacagttaca ggtggatcaa ttctttaccg
     7861 tcaacgcggt acaaaaatct atccaggtgt aaacgtgggt atcggtggag acgacacttt
     7921 atttgcaaaa gttgatggcg tagtacgttt cgaacgtaaa ggccgtaaca aaaaacaagt
     7981 gtctgtttat ccagtagctc aagaagctta attgaatctt atgattaaag aggttgtgac
     8041 aagacttttg tcacagcctt ttttgtttat aaaaataaaa gttgtagtgg aggctacgaa
     8101 gtttactttg tctttttaca cttaatattt ttttgccaat ttggaatcag gatgttttga
     8161 taactatcgt tttcaattga tggaagctat attgtttgct atcagtgaaa taattatatg
     8221 gtaaacagtg ttctttttct tgtttccatt ataaaaatcg tatcgggatt tttcgttctt
     8281 ttaaattgaa catttctatc tatccgcgtg ttatcaacaa aacaaaatca ggtaaatagt
     8341 tgacgaaaca gaaataaata catagtatta tgttttgaag cctaagtaca atagtctata
     8401 gaaaaggagc gataccattg gcaaaacgca ttgaccgaat ttatatgtat gtcaaagaaa
     8461 aaaccgctca tttaacaaag gcagaatatg atcaaggagt aacaacacag gaaattgctg
     8521 aagtgttagg gattcagcga acaaatagca gtaaagatct gaatcagcta gtgcgcgaag
     8581 gaaaactgat aaagacagac ggacgaccag tacgttacat atatcaaaca ctcgttacgc
     8641 acgtaaagcc agttacaaaa catgtagtca gttacaaaga aacgccggtc tctgaagaaa
     8701 aaaatacact ccaaatagat actaaggata tctttgcaaa aatcattgga gctaacggca
     8761 gcatgaaaaa ttcagtggaa caggcaaaag ctgcgatttt gtatccgcca agaggattaa
     8821 attgtttgat tacaggacct accggatcag gaaagactta ttttgcacac gcgatgtatc
     8881 attttgcaaa agcgcatcat gtaatagatg aggaaacgga actgattgtt ttcaattgtg
     8941 ctgattatgc caataaccct gagcttttga tgagtcattt atttgggtac gcaaaaggtg
     9001 cttttacagg cgcagatgaa gaaaaaatgg ggattatcga tcaagcggat ggcggtatgc
     9061 tgtttttgga tgagattcac cggttgccgc ctgaaggtca agaaatgatc ttttacttta
     9121 tggatcatgg aacttacagt cgtcttgggg aaacaacgaa atcccatgaa gcaaatgtac
     9181 ggatcgtagg tgcgaccaca gaagacccag gttcttctct attagaaact tttgttcggc
     9241 gtatcccaat caatatcaaa cttccggctt ttgaaaaacg tcctgcaaat gagaaagtcg
     9301 atttagtaaa aatcatgatt gcccacgaag caaaccgcac gcagcgaaag atttctttga
     9361 cagaagatgt agcaaaagca ttgatcggca gtgtgaccta tggaaatata ggacaattga
     9421 agtcaaatat ccagttagta tgtgcacgag gatttctaaa tcatatgcaa tcacccgaaa
     9481 tctcgatcac aatcgatgat ttgacagaag ggattcgtag cggattgatt caattagcaa
     9541 gcaaccgcag tgcaatggca gagctttcta aaatattgga gccaaagata actgtagcac
     9601 caaatgatac gatcatgaaa atccaatcgg actcctatga gttaccttat aatctatatg
     9661 atatcatcgg agataaagca gctttactga agtcggatgg tttggatcaa gaagcaatca
     9721 atcatttcat ttctacagat atcaacatcc atctgaagtc tttctataaa gaccatggtt
     9781 tttcttttaa tgcagacagc aaattagcag aatttgtaga tccgaaaatc atagaagtga
     9841 ccaatcagat ttatgtaatg gtcaaaagat ctttaccata tgagtttcag caaaacttta
     9901 tttatgctat gagcttgcat atcagttctt ttttgaaaaa aatcaactta ggagaagaac
     9961 ggcatacgaa tgataacatc cgtgagatgg cttttgattt tccagatgaa tatgctgttg
    10021 cgaaagaagt gcgtagttat atcgaaacgt attttcaagt ggagattcca gaaagtgaag
    10081 actattactt gaccgttcta ctcgtctcgt tacgggcaaa tcaagcaagc ggcagaatcg
    10141 gtgtagtcgt tgcagctcac ggaaacagca cagcaagcag tatggtccaa gttgtccaac
    10201 agttgttaga tgcagagaac gtacgagcag tagacatgcc tttggatatg gatccgaaaa
    10261 cagcattgga ccgaattgaa cgcaatgtac aagaagtaga tgaaggaagc ggggtcatac
    10321 tcttagtaga tatggggtcc cttgcgtcgt ttaattcgca aattcaaaga gacactggaa
    10381 ttccagtacg tactgttgat atggtaacaa cttctctagt tttggaaact gtacgaaagg
    10441 tctcagtatt agggacagat ctagatatgt tgcatgaatc attgaaaaat tttcgaggtt
    10501 acgcagaaat atcttctgta gaatctgttc aggaagcaaa acctattcat aagaaaaaag
    10561 ctgttttggc tatctgcgca tctggcgaag gtacagcaca aagaatcaaa gaattgatcg
    10621 aacgagcggt aaacaagcgt caagaaacag aattagaagt tctagctttg tcgattgtgg
    10681 aagtgaaaga agaactgcca gttatccaaa aagaatatca gatcatagct actactggga
    10741 ttactgattt gaatatccaa gccccattta ttcctttaga acgttttatt gatcaaaata
    10801 tcgaagtcat tttggatcag ttgctgatgg aatcagaatt ggaggaaact gaattcatct
    10861 ccctagatga agagtcagct aaaaatacgt gtgttgagtt tatctctgat aacttcatct
    10921 ttattaatgg tagtaagtta atcgatccaa tgtggcaatt ttctacacaa atctctcaga
    10981 cgacgggaat cggcgatgaa gaatatggat tcaaaatcaa cctagtgatg cacacagcag
    11041 gaatgatcga acgtattatt cggaatgaac cattgactgt ggaagaaaac gaattgacaa
    11101 atacaacgaa tgatcctctt tatagccaat tggcagcctc agttgttctg ttggaagatc
    11161 agataaaagt aaaagtgccg atagaagaaa tgtattatct gctacgtcta gtccataatc
    11221 agttagataa aaaagaatac actgtgccat gaatacacaa aaacgataca cttttatagt
    11281 gtatcgtttt tgtgtattgg ttaagaatcc tttaaattca attagtgtat cgtaaaaaag
    11341 ttggcacgct ttttgcttat agtagtgtgt gctaggaggt ggttgttcac acagaaatga
    11401 taggggtttc tgtgaagtag tgaggaagaa ggagttttgc agatggatat tcgattggca
    11461 cgtatagatg atcgtttaat ccacgggcaa gtcgcaacag tttggtcgaa gcaaacaggt
    11521 gttgaacgta tcattgtgat cagcgatgta gtcgctcaag ataaattgag aaagtttcta
    11581 ctaaaagaag ctgcaccgcc ggggatcaca gcaaatgtaa taaccgtgca gaaaatgatt
    11641 caaatctatc atagtggatt attatcacga gcaaaagtga tgcttttatt caccaatccc
    11701 caagatgttg aagcggttgt ccgctgcggt gtacagttga agtcgttgaa tatcggcggt
    11761 atgagtttta ctgaaggcaa atcaatgatt accaattttg tttcagtaaa tcaacaagat
    11821 attgattcct ttcgttactt gaacagtcaa gaagtggagt tagagattcg aaaagtacct
    11881 gctgacagga aaatcctttt gatggaacta ctagcgaaaa tcaaaaaggt gtagcaagaa
    11941 tggttgttgg aaaaggtcaa ataattctgt tgtaatggtt agcaattatt aatatttttg
    12001 aatttgttac aacaaaagta aataggaggt ataaatatgg tagggattat cctagcaagt
    12061 catggacaat ttgctgaagg aatcttacaa tccggttcaa tgatctttgg cgaacaagaa
    12121 aacgtcaaag ccgtgatttt gaaaccaagt gaaggtccag atgacttgag agccaaattg
    12181 gaagaagcgg ttgcttcttt tgataatcaa gacgaagtgt tattcttagt tgatttatgg
    12241 ggcggaacac cgttcaatca atcaaacaca ttattcgaag agcacaaaga taaatgggca
    12301 atcgtcagcg gtttgaattt accaatgttg attgaagcct acgcatcacg cttttcaatg
    12361 gaatctgctc atgaaattgc agcacacatc attgaaacag caaaagacgg tgtaaaagta
    12421 aaaccggaag aattagaacc ggcagaagca ccgaaagcag cagttgaaga cgcacaacct
    12481 aaaggagcac ttccagaagg aactgtggtc ggagacggaa aaatcaaata tgtattagca
    12541 cgtgttgact cacgtctgtt acatggacaa gttgcgacag cttggacaaa agcagtgcaa
    12601 ccaaaccgta ttatcgttgt ttcagatgca gtatcaaagg atgatctgcg taagagacta
    12661 atcgaacaag ctgctcctcc aggtgtaaaa gcaaatgtta ttcctattag caaaatgatc
    12721 gaagtagcaa aagaccctcg ttttggaaat accaaagcat tgcttttatt tgaaaatcct
    12781 gaagacgtat tgacagctgt tgaaggtggc gtagacatca aagaattaaa tgttgggtca
    12841 atggcgcatt cggtcggtaa agtcgttgta agtaaagtat tgtctatggg acaagaagac
    12901 gttgaagcgt ttgagaagct tgaacaaaaa ggtgtgaaat tcgatgtacg taaagtacct
    12961 aatgattcac gcgataacat ggatgatatt ttgaaaaaag caaaagctga attagcaaaa
    13021 gcgtaataaa taatattaaa atttatagga ggcttatcat gtctattatt tcaataattt
    13081 tagtcgtatt agttgccttt ctagctggta tggaaggtat tcttgatgaa tttcaatttc
    13141 atcaaccact agtggcatgt acattgatcg gtttagttac cggtaacctc gaagctggta
    13201 tcgtacttgg cggtacgctt cagatgatcg ctttaggttg ggcaaatatc ggtgccgctg
    13261 tagcaccaga tgctgcatta gcatcagtag catcagcaat tattttagtt ttaggtggac
    13321 aaggtgttaa aggtgttcct tctgcgatcg cgattgccgt tcctttggct gttgcagggt
    13381 tgttcttaac aatgatcgta cgtacaatcg ctgttccaat cgttcatttg atggatgcgg
    13441 ctgctgaaga aggaaacatc cgtaaggttg aaatgtggca catcattgct gtatgtttac
    13501 aaggggttcg tatcgcggtc ccagcagcag cgttgctatt tatcccagca caaactgtgc
    13561 aatcattcct tgaatcaatg ccagcatggt taacagatgg tatggctatc ggtgggggaa
    13621 tggtcgtagc tgtaggttac gctttggtaa tcaacatgat ggctacaaaa gaagtatggc
    13681 cattcttcgt tattggtttc gttgtagctg caatttctca attaacattg attgctctag
    13741 gtgctttagg tgttgcatta gctcttattt acttgaacct ttctaaaatg ggtggttcat
    13801 caaataatgg aggcggaagc aactctggtg accctttagg cgacattttg aatgactatt
    13861 aatcttgaag gaggaaaata aaatggcaga agaaaaaatc aaattatcaa aaaaagatcg
    13921 tttagctgtt gcgtggcgct ctaccttcat tcaaggttca tggaactacg aacgtatgca
    13981 aaacggtggc tgggcatttt ctatgattcc agcaatcaaa aaattataca aaacaaaaga
    14041 agaacgttct gcagcattaa aacgtcactt ggaattcttt aatacacacc catacattgc
    14101 ttctccaatt ttaggggtta cattggctct ggaagaagaa cgtgcgaatg gcgcgcctgt
    14161 tgatgatgta gcgattcaag gggttaaagt aggtatgatg ggtcctttag ctggtgttgg
    14221 tgacccagtg ttctggttta cagtacgtcc aatgctaggt gcactaggtg cttcattagc
    14281 tatgggagga aatatccttg gaccaatcat cttcttctta ggttggaaca ttattcgttg
    14341 ggcattcatg tggtatacac aagaatttgg ctacaaagct ggttctaaaa ttactgatga
    14401 cctttctggc ggattgttac aagacgtaac aaaaggtgct tcaattttag ggatgttcgt
    14461 attagctgcc cttgtacaaa gatgggtatc aatcaaattc ttaccagttg tatcaactgt
    14521 aaaattagat aaaggtgctt acatcgaatg ggataaattg ccttcaggtg gtgaaggaat
    14581 caagagtgca tttgaacaag ttaataatgg cttagccctt tctcctgaaa aggtaacaac
    14641 attacaagac aacttagacc aattgattcc tggtttagct gcattaggat taacattgtt
    14701 atgtatgtgg ttgcttaaga aaaaggtttc tccaatcgtt atcatccttg gtttattcgt
    14761 agtcggtgtt gttggacacg tcatcggact tttataatcc ataaaaatat agctcgggcg
    14821 ttgtcccgag ctttttttat atagtatact gttcataaac aaatatctgg agggagaaaa
    14881 tggttcaatc aatcaataca aaagtagact tggtaattga tgctacttcg tttacgggtc
    14941 tgacagatta cgggcaaatc atgataggtg ataatggttt tgagttttac catgcgcgag
    15001 atagacgcaa atatatccaa attccatggg aagaagtgga ttatgtacta gcatcagtca
    15061 tgctaaaggg aaaatggatt cctcgatatg ccattcaaac gaaaaaaaat ggtacataca
    15121 cattttcttc caaagaacct aaaaaagtgc ttcgtgcgat cagagaatat gtagatgaag
    15181 atagaatggt acaatcattg agtttctttg atgtagtgaa acgtggtatt cgtagtatgt
    15241 ttaaacgcaa gtaaaaaata aagaaaactt ctaagaggag gttttcttta tttttttact
    15301 ttgcagaaga aaatatgaaa tagcaaaacc aattatttgt cccctttaaa tcccatatgc
    15361 taaaattgat tagcaaagaa ggaggttata ttatgagttg gttatggaca ttgattgttg
    15421 gagccgtgat cggagcaatt gcaggtgcga tcaccagtaa aggaaattcg atgggctgtg
    15481 taacgaatat cattgctgga ctagtcggtt caagtattgg acaagcgatt ttcggcgaat
    15541 ggggaccaaa agctgcaggt atggcactta ttccatctat tttaggcgca gtgattttgg
    15601 tagcagttgt ctcatttttc tttggaaaac gatcataagt ttttacaaaa tagtaaacta
    15661 aaaggagttg cagcgagact gtgactcctt tttggcttta tttatttaaa ataaaaagaa
    15721 gttttccgga aatttttatt taactgtcgt gtacgaagaa aagtttgcta taatggaaaa
    15781 aagcgtgcaa agaaagaggg agtttcatta tgatgctacg tgtagaaaaa ttgcgcaaaa
    15841 aaatgcaaga agaaaatttg gattcatttt tggttaccag tccgtacaat cttcgttact
    15901 taacgaactt tacaggtacg acaggattag ctgttattac tttggaaaaa gcatttttta
    15961 ttactgattt tcgttatacc gagcaggcgg cagcgcaggc tcaagggttt gaaattatta
    16021 aaaatgtcgg acctattttt gatgaggtag caaacttagt acgcaaagaa gaactgcact
    16081 cattagcttt tgaggaaaca acagtctcat ttttagaata ttcggtttta gaagaaatca
    16141 tcgatgcgga attagtacct gtttcacaga tgatcgaaga actcagagaa gtgaaagatg
    16201 aagaagaaat cgctattatc gaaaaagcat gtcatattgc ggatatggca tatgatcaca
    16261 tcctgaaaat gatccaaccg ggcatgactg agattgaagt cgcaaatcaa ctagactttt
    16321 atatgcgttc tcttggcgct tcaggtgttt cttttgatac gatcgttgct agcggattgc
    16381 gttcggctat gccgcatggt gtagcaagca aaaaaatcat cgaacaaggt gacttgatca
    16441 cattggactt tggttgctat tacgaagggt acgtttccga tatgacacgt acattcgcta
    16501 ttggtgatcc tggggaaaaa ttaaaagaaa tttatcaaat agtcttagat gctcaattag
    16561 cagttattga tgctgcaaaa ccaggaatga ctggtgtcca attagatgca gttgctagag
    16621 attatatttc aaaacatggt tacggggaag catttggaca ttctacagga catgggatcg
    16681 gtttagagat ccacgaagga ccaaacgttt cttctcgtgc agaaaagcaa tttgtaccag
    16741 gaaatgtgat cactgatgaa ccagggatct acttaccagg aatcggtgga gtacgtattg
    16801 aggatgactt gttgatcaca gaagatggaa atcgtgtgtt gactcgttca ccaaaagaat
    16861 tgatcatctt atagtttcat caatagaaga agtaagcgta aaaatcagtg ttcacagact
    16921 ttgttataga aaatcactcg atttaatgat acactataga agaactttta agctgtaaga
    16981 gataataaag ttgggtgttt tctccaattc atagcagttt gcagctgatc ggcggcagtt
    17041 ttcttaccac gtgatcacta tgccgcatca tagagaattc ggatgtatct cgtgcttagg
    17101 caaagggagg aataaataaa tggcagatga aaaaaatttg attttaaacg caaaccaaga
    17161 tttaggagag atcgtgatcg ctcctgaagt catcgaagta attatcggga ttgcagcttc
    17221 taaagtagaa ggcgtatacg gaatgcgcgg aacttttgcc agcaatgtca ctgaattatt
    17281 aggacgtgca gctcacggaa aaggtgttta cttagcaaac gaggaagatg gcttgaaagt
    17341 agatctttac tgttatctag aatatggtgt ttcagttcct aaagtagcaa tggaaatgca
    17401 ggaacgggtc agccagcaag tccttttcat gacggatatc gatttatctg aagtcaatat
    17461 ccatgtagta gctgttgttc ctgaaaaatt agaacaacca acaatcgaag aactttttga
    17521 agacgaagag gaagagaatg aataaagaat tatcaagaca cgagattcgg gaaatggcat
    17581 tgcaggcttt atttccatta gacttcaatg cagacctaac aaaagaagat gcgattttca
    17641 acgcaatcga attagaccac cgcgacatga tcaacgaaga tgaatcagag tttgttcctg
    17701 tttatcttga tacgctagtt ggcggtgtct gtgcaaaaaa agatgaattg gataaagtga
    17761 ttgaaaaaca tttaaaaaac aattggcaca tcaatcggat ctcaaaaatg gatttggtga
    17821 tcttgcgtat cgcgattttt gaaatgactt atgtgacaga tgtaccagcg gctgttgctt
    17881 taaatgaagc aatcgagttg gcaaaaacat ttagtgatga ccgttcacgt aaattcgtca
    17941 acggtgtttt atccaatgta ttgaaagaat tagaagctgg ggcgtaagtc ctagcttttt
    18001 tctatgaatc atcataaacc tgttataaat cgggaaaatt ctatggtaga atgaaaacac
    18061 aaaaaatatc tgggagtggt tagatggcag aattgattaa cggaaaagaa ttagctgaaa
    18121 aaatgcaggc agagattgct gaaaaaatcc aaaaattgaa agataatggt atccatcccg
    18181 gattagtcgt tttattggta ggggaaaatc cagccagcca aatctatgta agaaataaag
    18241 aacgtgtagc aaaagaaatc ggaatccatt cattggtaga gcgttatcct gagacgattt
    18301 ctgaagacga actacttgca gaaattgaaa aatacaatca agatcctagt tttcatggta
    18361 tattggtcca attaccattg ccaaagcata tagatgaaga aaagatcctg ttagctatcc
    18421 gccctgaaaa agacgtagac ggttttcatc cgatgaattt aggcagactc cttgcgggta
    18481 aaccggacaa gattccttgt actccatacg gcatcatgaa aatgtttgaa gcttattcta
    18541 tcgatccggc gggtaaacga gcagtagtta tcggacgaag caatatcgtc ggtaaaccga
    18601 tggctcagct actcttgatg gcagatgcaa ctgttactgt tgctcattct aaaaccgaaa
    18661 atctggctga actggcacaa gaagcagata ttttagtcgt agcaatagga cgtggacatt
    18721 ttgtcacaaa agaattcgta aaaccaggtg ctgtagtgat agatgtaggg atgaatcgtg
    18781 atgctaacgg taagctgatc ggtgatgtga aatttgatga agtggagcct attgcaggct
    18841 ggatcactcc tgtgccaaag ggtgtagggc caatgactat cacgatgctg atgtatcaaa
    18901 ccgtaaaatc tgccgaagct tttggagcag gtgaatagat ggcacaagaa tatttaactg
    18961 tcactacatt aacgaagtat ttgaaaagaa aatttgatgc cgatccttat ttagaacgcg
    19021 tatatttaac aggcgaaata tcgaatttcc gtttacgtgc caatgcccat caatatttca
    19081 gcttaaaaga tgaccatgca aaaatatctg ctattatgtt caaaggagca ttccaaaaat
    19141 tacgattcca gccaaaagaa ggaatgaaag tccttgtcgt tgggcgtatt tcattatatg
    19201 aagcgggagg ttcttatcaa atctatgtag agcacatgga gcctgatgga ataggtgctt
    19261 tatatcaaga attggaagaa cgaaaagaaa agctgatgaa ggaaggcctt tttcaagggc
    19321 ctaaaaaaat gctgccacgt ttcccaaagc gaatcgcggt ccttaccagt ccaagcggag
    19381 cggttatccg cgatatcatc acgacggtga aaagaagata tccaattgtc caattggttt
    19441 tgttcccaac agttgtacaa ggaaaccaag cagcggacaa tgttgtcagg aatatccaga
    19501 gagtagaaga gatgggcaat ttcgatacaa tgatcatcgg tcgtgggggt ggttcgattg
    19561 aggatttatg gccgtttaat gaagagcggg ttgctcgggc gatttatcaa gctaatacgc
    19621 cggtgatttc atctgtagga catgaaacag ataccacgat cgctgattta gtagcagatg
    19681 ttcgggctgc cactccaaca gcagctgcag aattagctgt tcctgttttg tcagaagagt
    19741 tgatgcgcat tcaagaacgg caagctagat tgcagcaggc gtttgtacgc cagatccagc
    19801 gcaaacaaga acggttcgag cgtgctcgac gttcctatgt atttcggcag cctgaaagat
    19861 tgtatgaagg tcagaccatc aaattagacc agttgaaaca gcgattatat caagctacac
    19921 agcaaattta tcatgcgaaa gaaaaacaaa cgatcgcatt aagccaccaa ttggaacaag
    19981 tggctccgct ttatcgagtt aaagcagctc gccaagaaac tgcttatcta gaaaaacgct
    20041 tagaagagaa aatccaacaa tatatgaaac aacagcagca gcgttttgct caagctgttc
    20101 aatctcttga tttgttgagt ccattgaaaa tcatgggaag aggctacagc tatacgacgt
    20161 taaatgatca agtagtaaaa agtgtcaccg agattcatca aggggatttg ctgacgatcc
    20221 attatcaaga tggaatcgtt aaaggacaag tacaagagat agacaaagga gaataaaaat
    20281 atggaaaatc caacttttga agagtcactg caagaattag aaaaaattgt catgcagtta
    20341 gaacaaggag acgttccatt agaatcagct ctagattcgt ttaaacgagg aatggaatta
    20401 agcaaacatt gccaagatac attagctaaa gcagaagaga ccttgacgaa aatgatgaca
    20461 gaatcaaatg aagaagttga ttttgacgga aacgaggaaa gctgagatga gattaactga
    20521 cttttcagct attcatctac ctgctgtaga aaaagaaatc ttgtcttttt tagatgagca
    20581 tacaacggat agaggactct atgatgcgat gagttattca gtgaaagcag gcgggaaaag
    20641 aatacgtccc ttattacttt taacggccgt tgcttccttt gatgagccta ttgatgttcc
    20701 cgtctatcaa gtagcagctg ctttagaaat ggtgcatacg tattccttga tccacgatga
    20761 tttgcctgcg atggacaatg atgatcttcg tagaggaaaa cctaccaatc ataaagtatt
    20821 cggcgaagca ttagctatat tggccggaga tggtttgctt acaggagcat tccagctaat
    20881 cagtatggca cacttaggta attctcctaa gcttctgctg cttcagcaat tagctgtttg
    20941 tgcgggatca caaggtatgg ttgctgggca agctgcagat atcgagggcg aatcgaaaaa
    21001 gctttcttta gaagaattag catttattca tgaaagaaag acagggcatt tgattcgtta
    21061 tgccttgctg gctggtggca tattagcgaa gcaacctgaa gaaatcttat tgctacttca
    21121 acgattagcc gaacatttag gtttagcttt tcagataaga gatgatctac tcgatgtgat
    21181 tggtacaacg aaaacattag ggaaaacagc tggtaaagat gaacgtatgg aaaaaaatac
    21241 ttacccgcgt ttattaggcc tagaaaagac acgtgaggcg ttggagattg agctgttatc
    21301 cgccaacaag atcatagaca aattagaaga ggacgttttt tcttttgatg gcgggctgat
    21361 gcgccagatg atcaagcaat ttgatgtgga gaaatgaggg aaagaatgga aaaagaacgc
    21421 gtagatgtat tagcggtcaa acaaggatta tttgaaacaa gagaacaagc caaacgttca
    21481 gtgatggctg gtttgattta caacgaaaaa aacgagcggt ttgataaacc gggagaaaaa
    21541 attccagtga gtagtgaatt aagagtcaaa ggtaaaaaac ttccttatgt ttctcgaggg
    21601 ggattaaaat tagaaaaagc actcaaagca tttgatctat ctgttgaagg aaaaacattg
    21661 ttagatattg gtgcatccac tggcggattt actgatgcag ctttgcaaaa tggcgcaaaa
    21721 atgagttatg ctttagacgt tggctacaat cagctagctt ggaaaataag acaagatcct
    21781 agagtcgtcg taatggaaag agtgaatttt cgttatgcaa aacccgaaga ctttacctta
    21841 ggaattccag aagttgctgt tatcgatgtt tcttttattt cactaaaact gatgctgcct
    21901 ccattacatg ctattttaaa agaagaggga gaagtgattg ctttaatcaa accccaattc
    21961 gaagcaggca gagaagctgt agggaaaaac ggaattgttc gtgaccctca gacccatcaa
    22021 aaagttttag aagacatttt gtcatttgcg gcacaaggtg gatatgatgt gctggaatta
    22081 agttattctc cgatcacggg gggagaagga aatatcgagt tcttggctca cttgaggaaa
    22141 gtacccgaaa gcggaacaat caattcagct attaatatgg cagaagtggt ttcaaatgca
    22201 cacgaacagt ttgaccataa ataaattgta actgtgaaaa tcaagtgaga agtatgcgga
    22261 ttcttcttta ttataccaag gaaataagct atcattgatt aagaagaata tgctaaggag
    22321 cgaagacgta atgagaaaaa aagatcggca tcgtttgatt actcgattgt tgagtgagca
    22381 agatatccga aagcaggaag aatttgttga aatcctgaaa agcaaaggaa tatctgttac
    22441 tcaagctacg atttccagag atataaagga actgaaactg atcaaggtac cggcttcaga
    22501 tggcggctac cgttatagca taccagctga aacaaacgaa gatgtaaata ctaaattgga
    22561 aaaactatta aaagacgctt tcgtggcagt tgaccaaatg gaaaaatttg ttattttgaa
    22621 aacattgccg ggaaatgctt cggcagcagc aaatttgatt gataaacgtt acaagaaaga
    22681 attattttcg attatcaatg atgacgataa tgttttgatg attacacgaa ctgaagacca
    22741 ggcagtttta ttgaagaaag acttcctgca ttacctttaa gttgaggagc gaatgtgatg
    22801 ctacaagaaa tcagtattac taactttgcg attattccgg aattgcggct ttcttttcac
    22861 gaaggaatga ccgcgctgac cggagagact ggtgcgggta aatccattat tatcgatgct
    22921 ttaggtctgt tagcaggagg aagaggttca agcgattata tacgtcaagg agcagaaaaa
    22981 tgtgtgcttg aagggttgtt tgaactacca aaacaagaag gattctctga actgatggtt
    23041 gaattaggga tcgaaacaga tgaggacaat ctgattgttc gacgggatat gtccttaact
    23101 ggaaaaaatg tttgtcgagt gaatggtcat attatcacat tagctaatct gagaagaatc
    23161 ggcagttatt tagtagatat ccaaggacaa aacgaacatc aggaactgct ccagcctgaa
    23221 tcacatcttg ctttattgga tcgttttgga gatgtagctt tccaacagaa gaaaaagagt
    23281 tatcagcaag aatatatctc ttatcgtgag ctggaaaaaa gggtacgaaa agtgcagcaa
    23341 aatgaaaaat cctatgttca gagaatagat atgctccatt tccagcaaga agaaatagct
    23401 gctgctgatt tgcaagtcgg agaagaagag aaattaaaag aagaaagaga aaaacttagc
    23461 aattatcaaa aaattgctga tggattagct gcaggttacg gagcactgac gaataatgaa
    23521 caaaacagtg tagatggtgt tggcttggcc gtttcagaac tgcaagggat cgcccatcta
    23581 gatgtggagt atgaagcaat ttatgaaaat atccaaagtg cttactattt gctgcaagat
    23641 gcaatcggcg atatgagtcg tcagattgat ttattggagt tagatgagag tcgtttagaa
    23701 gaagttacgc aacgtttaga attaatccga cagttgaaac gtaaatacgg cgaatccatt
    23761 gagtcaatat tggcttacta tgacgaaatc accgaagagc tagcttcttc tgatttttca
    23821 gaaggtcaat tggacaaaat gaaagaggaa ctagagcaaa aagagcttct acttcagcaa
    23881 caagccgcag atcttcatga agcgcgtaaa aaaatcgcaa aagaattgga aaaatccatt
    23941 ctgcatgaat tgaaaagtct gtatatggaa aacactgagt ttgaggtacg tttcttaaaa
    24001 gaagaaaata gacaattgaa tcatgatgga tttgaccaaa tcgaatttta cattacgacc
    24061 aatccaggag agccactgaa gcctttggtg aaagtcgctt ctggtggcga actgtctcgg
    24121 atgttgcttg ctttgaaaac tattttttct tctgagcaag gtgtgacaag tattattttt
    24181 gatgaagtgg atacaggtgt gagcggaaga gttgcacagg ccatcgcaga taaaatttct
    24241 caaatatcga aatactctca agttttgtgc atcactcatc tgcctcaagt tgctgcagtc
    24301 gccgactatc aatattatat tgtgaaagca gtgattgatg gacgtacgca aacttctgtt
    24361 tcagagttga aaacaaaaga aagagaagaa gaaattgcac gtatgctggc tggaagtgag
    24421 attacaaaac tcacactcga acatgcaaaa gaactattga aactcgcaaa aaggacctga
    24481 gccacctcgc agtggttcag gtccttttca gcagctgata gctatatttt atttttacat
    24541 gacactcatt acagcagtca atacaacaga tccaatcgtt aaaatcagca ttgtccaaat
    24601 gacaaatttt gttactttag caaaagtact tgtttgtttt ttttcacgca ttgttttcaa
    24661 cctcgtcatt aattagttga ttcaagtgta caatcttttt tccatttcca caagagggct
    24721 tttttataaa aacttccctt tttttaaata ataggtagtt acgatacaaa taaataatgt
    24781 taatagaact agccatataa aggcatcatc cctttcagca ataggtaact taatgttcat
    24841 tccatagata ctgccgacga ttgtcggtat agttaaaata atcgttaaag aagttaaaat
    24901 tttcataata ttattcagat tattcgatac gattgctgaa aaagtctgat tcatttgttc
    24961 tactagtttt aattgaatgc gagaagtagt cattgcctgc tctgtttcca ccaatacatc
    25021 acgtaaatga tttttcaaag catgattttc ttggaaaagt ttattttgcg ctagttcttt
    25081 caatgcatct aaattagcag aagtcgcttc tctgaaataa acgaggcttt tttggatatc
    25141 catgatttga taaagttgtt tgttttcagt ggatacttga agtttggctt ccaaagtatc
    25201 gcattgagat tgaagcgtag ctaaataact gttgtaagag agtgtgacct gccaaatgaa
    25261 ttgaaagaag atattgagat taggcgaaat atcacttttt ggaaaggtct ggctaaagac
    25321 tttctttaaa aacagtggtt catgattcgt aattgtgata agcttctttt tgggcgtcaa
    25381 aataatagag agcgggtatg tatccacttg aaaataaccg ctaggacttg ttttagcaaa
    25441 aggatattgt aacaataata acgctggctt tgtcaacttt gattgattga attcttctgt
    25501 acgggaattt tctgcatcgt ccaatatgga agtgatgtaa tctgacggaa tttcaaattg
    25561 atgtgtcagc tgatgaatct cttcttgatt aggacgttct acacaatacc agatcgtttg
    25621 gtcagtttcc tttccggtat tacgcagttt ctcattatct aatgtaaaat atttgatcat
    25681 gattttcctc ctgttgcctg tttcttttat tataacggat agggaattgg aatacatgga
    25741 atgtgtttgt aagatataga atataactgc ataaatttaa ataaaatgaa acagttgtgt
    25801 aaagtatata cagtatactt gaagcaacga taaaaaagga gtttacgttc atgggcacat
    25861 tcacaccact tatcttgttt tggtatattt acccggtaat cgttttgttt ggttgccaat
    25921 ttcttgtaaa attattttcg ttaaatcgtc gttttaaaat aaaagcccct gatttggcga
    25981 ttcctttttt gtggggcggt atgcatgcat tgtctagaaa tacagggaca atttctatct
    26041 taccgttttt ctttatttct gttttgctaa tgggaatttg tattgctgtt ttccaagcat
    26101 attattatga agaaattatt tatccgcgtt atttcaagat gacttggcgt ttggtttttc
    26161 tgttgacaat cgtcttgtac gctgtcttga tcattgtgaa catactttct tacctatgaa
    26221 aaaaatagaa aagctgtaaa aagtctttta aaaggctttt tgcagcttga tttttaaaaa
    26281 aatgtttatg aataaaaaaa gttgttcccg gatttcccag aaaaggtggt agaaagtggg
    26341 ggattgtggt agactaattt tagttagtgg aggaatgggg ggctttcaat gttgatgggt
    26401 gaattccaac ataatattga tgcaaaagga cgtttaatcg tgccctcaaa acttcgtgaa
    26461 gaacttggcg aaaagtttgt tctgacacgc ggattggatg gctgtctttt cggctatccg
    26521 atgagcgaat gggaaaatct ggaagctaaa ctgaacgaaa tgccgttggc taaaaaagac
    26581 gcacgtactt ttgtgcgatt cttctattcg gctgccactg aatgcgaact tgataaacaa
    26641 ggacgaatca atatacccag cacgttaaga aattatgcag ctttgacaaa aggttgcgta
    26701 gtcatcggtg tatctaaccg aattgaaatt tgggatgaga cacgatggca agatttttct
    26761 gcagcagcag aagagaactt tgacgaaatt gctgaaagta tgattgattt cggactataa
    26821 gaataggaag aggctgacta atgactgaaa catttcaaca ctatacagtc atgttgaaag
    26881 aaacggtaga tggattgaac atcaaaccgg atggcgttta tgtagactgt acactcggcg
    26941 gtgccggaca tagtgaatat ttattaagcc aactaagttc taaagggcat ctgtatgcgt
    27001 ttgaccaaga tcaaaaagca atcgatcacg ccaagctgcg tttagcttct tatgtcgaac
    27061 aaggtatggt cacatttatc aaagcgaact tcagagaatt agaagaacgc ttgagtgagc
    27121 atgtagataa ggtagacggc attttgtatg atctaggggt ttcctctcct cagctagatg
    27181 aagcagaacg tggatttagt tatcatcaag atgcaccgtt ggatatgcgc atggatcagt
    27241 cagctccttt atccgcttat cacgtaatta atgaatattc ctaccatgaa ttagtaaaga
    27301 ttttcttccg ctatggcgag gagaaatttt ccaaacaaat tgctcgtgaa atcgaacgag
    27361 tacgaaaaac aaaaccaatc caaacaactg gtgagcttgt cgaaatcatt aaacaagtca
    27421 tcccagctcc cgcaagaaga aaaggcgggc atcctgcaaa aagaattttt caagctgtgc
    27481 ggattgcagt aaatgacgaa ctcggtgccg aagaggcctc tttagaacag gcaatccggt
    27541 tattgaaagt caatggaaga atcagcgtaa tcacattcca ttcattggaa gacagaattg
    27601 tcaaaagtat gtttaaagaa tatagtacgg tacaagatct accaccggga cttcctgtca
    27661 tacctgacga attccaacca gaactgaaag tcattactcg taaaccaatc cttccttcag
    27721 aggaagagct aacagaaaac aatcgatcac gcagtgcaaa gttacgtatt gcagaaaaaa
    27781 taagaatgaa agaggagtag aatgatggca gaattaaaga aaatgcaaga ttacgaatat
    27841 gatttacctg aattggtgga tcagccggaa caacaaatgc ctgattcttc ttcgaatcaa
    27901 gaaatcattc ttccacaatc accggcaaaa cgattaaagc agatttctgc tttggaaaaa
    27961 attgctgtgg caagcgtggc gattgctatt gttgctttat gtgttttaac agttatgctg
    28021 cgcacaaata tcagcggagc tgaaaaagaa atcacgtcaa tacaaacaga aatcaatgat
    28081 aaaaagcaag aaaaaacgag tttagaacaa gaaaaaaacg aactttctcg tactgaacgg
    28141 attaaacaaa tcgcagaaga aaaaggattg tctattaatg acgacaattt aaggagagtg
    28201 aagtaaatga gtcttaaaaa taaaattcgg cggtttatgg aaaagaaaaa tctaaatccg
    28261 atgaacaatc gcaaaaaagt aggtatcatt ttatttgcta cgagtattgg attgttcttt
    28321 ttatttgccg tccgtttctc gtatatcgtt attggaggtc atgttgctgg tacctcatta
    28381 gctgaaaaaa caaaacagtt gtaccaagga agtgaagtag taaaagcaaa aagaggaacg
    28441 atttatgatc gtaacggtgt ggcgctcgca gaagatgcct cctcttattc aattaaagcg
    28501 atactctcaa aaacatatac ctctggggat aaaaaactgt atgttgaaga aaagaatttt
    28561 gataaaatag cagaaatcct gcataagaat ctttctatag acaaaaaaga tgcactgaat
    28621 attttagagg acggcgcaaa aaaagagctg tatcaagttg aatttggtag ctatgggaaa
    28681 aatatctccc aagaaaccaa gcaaaacatc gaagcggaca tgaaaaaaga aggtgtcgct
    28741 ggtctttatt ttgtcgacca tcaggctcgg atgtatccaa atggcgtctt ttcttctcat
    28801 tttattggat acgcagttcc agacaaagat gagaatggct tagttggtaa actaggtttg
    28861 gagtctgctt acaatgatat cttgagcggt aaagatggta agattattta tcaaaaagat
    28921 aattttcaaa atcctctgcc aggtacagtg gcagaagaag aaaaagctgt cgacggacaa
    28981 gatatctaca cgactttaga cagcagacta caaagttatt tagaaacact gatggatcaa
    29041 gtaaatgaag aatatcagcc agaagaattg acagcagtcc tgatgaaagc aaaaaccgga
    29101 gaaatcttgg caatgggaca acgacctact ttcaatcctg aaacaatgga aggattgact
    29161 ggaaaagatg ctatctggcg taatttcttg gtacaagata gttatgaacc aggatcaacc
    29221 atgaaagttt tcactactgc agctgctatc gaagaaggag aattcaacga gaatgaaacc
    29281 ttccaatctg gaaaaatcca agtagaagat gctacgatca atgaccatga ctttggtgaa
    29341 aaaggtgtgc tgaccatgcg tcaagctctt tcatggtcaa gtaacgtcgg gatggtcata
    29401 ctggaacagc gattaggcgg acgatggtat aactatttac aaaaactggg atttggacaa
    29461 agtactcatt ctggattaga tgatgaagta aacggtgctt tacctacatt aaatattgtc
    29521 gaccgagcaa tgagtgctta tggacaagcg gtaggtgtga cgaatttcca aatgatgaaa
    29581 gctttcactt ctattgcgaa taacggtaca atgattcaac ctcgatatat cagcaaagtg
    29641 gtggatcccc aaactggaga agagcgaaca actcaaacag aagttctagg gcaacctttt
    29701 tcaaaagaaa caacagaaaa agtccgtgaa tatatgagag atgttgtcga aagcgaaaac
    29761 tacggtagtg cttatggtgt atatagtgta cctggataca atgtatcggc aaaaaccgga
    29821 acagcccaaa tcgcttctga tacaggggga tatcaaacag gagatacagc ctacctgtat
    29881 tcgattgtgg agatggttcc ttctgaagat ccagactatg tcttgtatct aacaatgaaa
    29941 caccctaaga catatgatcg aatggcattg gctaaaatcg caaatccatt gatgaaacgg
    30001 gcaatggatt ttaaagaaac tgaagaagat tcagatacag aaacaaaaac agaaaaagta
    30061 tccgtagccg attatcgtaa tcttgaagcg gacgttgcag cagctgatgc tcaaaaaagt
    30121 ggactgcaac ctgtagtgat cggtaatggt aaaaaagtac aaaaacaatc tactgccaac
    30181 ggtgatcagt tgatctctgg agaaaaactg atcctatata ctggtggtga taagctgatg
    30241 cctgacgtaa caggatggtc aaaagcagat atcatgaagc tggggaaaat cctcggtatt
    30301 gaagttagtt ttgatggaga tggttattgt gtcaaacaag aacttgctcc atatgaaaaa
    30361 atcactgaag acaaacttag ttttacttta gaagaataag aagagaaata ggagagaaaa
    30421 gagcatggaa tggacacaag cattgattcc cattgtcagc agttgtgcaa tgacaattgc
    30481 cgcaatgccg ctatttattg gttattttca aatgaaaaag caaggtcagg cgattcgtga
    30541 ggagggacca aagtggcaca atagcaaagc cggaactcct acaatgggcg gcttagtgtt
    30601 tctgatcggc tctattttga caggaatttg ggttggagct tggcaaaaac agctaactcc
    30661 tactcttttc atcttgcttt ttgtattagc gttatacgga gtaatcggct ttcttgatga
    30721 ttttataaaa atattcaaga aaagaaacat gggactgaac tcgaagcaga aattgattgg
    30781 acagattcta gggggaatca tcttttatct tgtttaccgt tctgaaggtt atccaggtgt
    30841 attgaatttc ttcggactag atctaccttt aggaatcatt tatggtattt tcgctctctt
    30901 ctggcttgtt ggattttcaa atgcagtaaa tctgacagat ggcatcgatg gattagttgc
    30961 tggcttagga agtatctctt ttgcagctta cggtctaatt gcttggcatc agcatcagta
    31021 tgatgtatta atcatttgct taagtgtttt aggaggttta ctgggtttct tcgtctacaa
    31081 cagaaaacct gccaagattt ttatgggaga tgtcggttca ttagcgttgg gcggactttt
    31141 agcggcaatt tctataatgc taaaccaaga atggacattg cttttgattg gtttgatcta
    31201 tgttatggaa acagcaagtg tgatgcttca agttacttcc tttaagctta caggaaaacg
    31261 aatttttaaa atgtctccaa ttcatcatca ctttgaaatg tgcggatggt cggagtggaa
    31321 gattgataca gttttttggt tgatcagcat tgtgacttcg ttgatcacat tatggatcgt
    31381 ttggtaggag atgacgggaa atgaaaaaaa tatcaacata tgaaaataag aaagtgcttg
    31441 tcttaggact cgcaaaaagc ggagtaagtg cggccaaatt attacatgaa ttgggcgcac
    31501 ttgtcaccgt taatgatgga aaaccttttg acgaaaatcc ggaagcacaa gagctgcttt
    31561 ctttagggat aaaagtaata acaggtagtc atccaatcga attgttggat gaagaatttt
    31621 cattgatggt caaaaatcca gggattccct acagtcatcc tttagtagca aaagcccaag
    31681 aaatgggtat tccggtaatt acggaagttg agctagctta cgaagtagcg gaatgcccaa
    31741 ttattgggat tacaggaacg aacggaaaaa caacgacaac aacgatgaca ggactactac
    31801 tgaatgcagg agcagatcaa ggaattgcac gtttagcggg taatattggg tatccagcaa
    31861 gtggtgtagc gcaagaagca aaatcagaag ataaaattgt gatggaattg tctagcttcc
    31921 aactcatggg aatcacagat ttccgccctc atatagcggt gattaccaat atctatgaag
    31981 cacatattga ttatcatggt acaagaaaag aatatgtcaa agctaaatgg aaccttcaaa
    32041 aaaacatgac agaaaaagat tatctgatcc ttaactggaa tcagtcagaa ctacaagaat
    32101 tagcgcaaag aacaaaagca cgcgtactgc ctttctcgac aaaagaagtg ttggaagacg
    32161 gtgtgtatgc tgacgattac tcaatatact ataaaaaaga aaaaataatg gaaatatctg
    32221 aacttggcgt accaggtaag cataatgtag aaaatgcact agctgctatt tctgtagcaa
    32281 aattgtatgg tatatcaaat gaagctatac gagaaacgtt gcattttttc catggcgtac
    32341 cacatcgtac gcaatatgta ggagaaatcc aaggcagaaa attttataat gattcaaaag
    32401 caaccaatat tttagcgact aaaatggctc taagtggatt tgaaacgtct aaggtcgtgt
    32461 tattggctgg cggattagac agaggaaata catttgatga attaattcct tcattaaaag
    32521 gaatcaaagc aatggtcgta tttggccaaa caaaagaaaa gttgatggat gctggaaaaa
    32581 aagcaggcat cgagacaata gtaacagccg attctgtaga acaagcagtt cctttagcat
    32641 tagaaaacag tacagatggt gatgtagttt tattatcacc agcgaatgcc agctgggatc
    32701 aatacccaaa ttttgaaact agaggaaaca gattcatgga agctgtgaac cgattaaaat
    32761 agaaaaagta gaagcgagcg ataaaaaatg aaaattttgg ttacaggcgg aggaacaggg
    32821 ggacatattt atccagctct tgcctttgtc aattatgtaa agactcaaga accgaatgct
    32881 gaattcatgt atgtgggagc aaaacgaggt ctagaaaaca aaatcgtccc tgatacagga
    32941 atgcctttcc ataccttgga gattcaagga tttaaacgaa aaatttccat gcataacatc
    33001 aagacggtcc gattgtttct taagagtatc cgtcaagcaa aaaaaatcct cagtgatttt
    33061 caaccagata tcgttatcgg tactggagga tatgtttccg gagcggtagt gtacgctgca
    33121 gccaaaatgg gtattccaac agttatccat gaacaaaaca gtgtaccagg tgttaccaat
    33181 aaatttttaa gccgatatgt ggatcggatc gctctttcat ttaaagatgc ggcttccttt
    33241 tttcctgaaa acaaagcagt tcttgtaggt aatcctcgag cacaagaagt agcagatact
    33301 aaaaaatcag atattttgaa aacatttgat ttggatccag aaaagaaaac agtcttgatt
    33361 tttggcggta gtcaaggagc acttaaaatc aatcaagcag tatcagtttt tttaagtgaa
    33421 tatattcctt cttcttacca agttttgtat gcttcaggag atagatatta tgaagagatc
    33481 ctaaataaaa taaatcctga aatacaaaat attagtgtcc agccttatat caaaaatatg
    33541 gcagaggtca tggcttccag tgatctgctg gtggggcgtg ctggtgcaac ttcgatcgca
    33601 gaattcacag ctctggggtt accggcagtg ttggttccca gcccatatgt tacgaatgat
    33661 catcagacta aaaatgcaat gagtcttgtt cacgcaggag cagcaaaaat gattgcagat
    33721 aatgaactaa caggtgaatc actaagtcag acagtcaacg aaatcatggg tgatgaagaa
    33781 ttgcaaaagc aaatgtgccg agcttctaaa gaacaaggaa tccctgatgc ttcaaaacgt
    33841 ctttatgact tagtaaaaca aataatcaaa aaataaaggc ggtggtctta gtgagtaaga
    33901 aaaaagaacc aaaaaaattg tctcatccgt cttctgatga aaacaatttg actccttggc
    33961 aaaaggctaa ccgaaaatat ctggcagaac aagagatgaa aaaccaagag ccaaaaaaag
    34021 aagacataac attagataca cccctttcag attctgaaga agaaaaaacg gttcaagagg
    34081 aacagcaaga agaaagccag tccgagaaaa aaatcgaatt ttttgaagag atcaagcaaa
    34141 agaaaaacgg tggaccatat aacggttctt ttttgaaccg gctgcccaat ttaaaaagtc
    34201 aacggcacaa agtactattt cgtcggttag ctttgatcat cactgtattg ggtataccgc
    34261 tgattttttt gatctattat gtttctcctt atagcaaatt acaagcagtt gcagtatcag
    34321 gcaataagac agtcaacagc caagaaatca tctctgatac aaaattgtca ttaggagaaa
    34381 atgtttgggg acagtatttt catcgatcta cttatatcga tcgccttaaa aaagcacagc
    34441 caagaatcga aacagcaaat atacatttca aagggatgaa tgaattcgat ttggatgtaa
    34501 cagaatataa agagattgct ttgatcgcaa aaaacaatca atactaccca gtgattgaaa
    34561 atggaacagt attagatgaa aaagtagcaa atcctacaaa aaatctccct atcttggaag
    34621 agttcaaaga ctctgcaaaa atcaaagaat taacaaagca atacaatcag ttatcatcag
    34681 aattacaaaa agcaatttct gaaatcaagt acacgccaag agcaagcaat aaaaatctga
    34741 tccaattgaa tatgaatgac ggcaatcaag ttattgtaaa tatcaataat cttgcgaacc
    34801 aaatgaaata ttattcgcaa gtagccaaag atatggatga aaaaggtgtg atcgacatgg
    34861 aagttggtat cttctcgcat ccttacggta gcactcaaga aacggaagaa tcgggccaat
    34921 cggaagaaac aactgaaagt gaagaaaatg cggatcaaac aaacgaatca agtgaagagg
    34981 cagaaacgca aaaagcacaa acacctggat cgtctagcac aaacggatca tctgattcaa
    35041 atgattcaag tgaaacaagt caaatagaag ataatccaag tacaactccg tcaagtttaa
    35101 atgaaaattg ataaaacaac tttaaaaagt aagaatgtac tttctaaaaa ccagtaattt
    35161 atggtagaat tggaagcagt gaatatatct attaataagt atattttaaa acggacgcta
    35221 gttgttcgct tgtatatgga atagtaagta ggagggaatc ccattcatgg caaaaacagg
    35281 aatgtatgta ggccttgata ttggaacgac atctgttaaa gttgtcgtgg ctgaatatat
    35341 cgacagccaa atgaatatta ttggagtagg aaacgcaaaa tcagaaggga ttaaccgagg
    35401 cattatcgtt gatatcgata aaacggtcca agcgatacaa cgagcagtac gacaagcaga
    35461 agaaaaagca ggtatccaga tcaaaggcgt aagtgtcggt ttgccagcaa acatgcttga
    35521 agtagaaaat tgccagggaa tgattgcagt taacggagat tcaaaggaaa tcaccgatga
    35581 agatgtcaga aatgtggctt cagctgccct tgttcgttca atacctcctg aacgtcaaat
    35641 cgtgtcaata ttgccacaag attttactgt cgatgggttc gagggaatca aagatcctcg
    35701 tggaatgatc ggcgtaagat tagaaatgta cggtctgttg tttacaggac ctaaaacgat
    35761 catccataac atacgtaaat gcgtggaaaa tgccggatta atcgtcaacg aaatggtgat
    35821 tactccttta gcgctgactg aatcaattct ttctgacgga gaaaaagatt ttggtacgat
    35881 cgtcattgat atgggtggcg gccaaacaac aacggctgtt atgcatgata agcaattgaa
    35941 gtttaccaat cttgaccaag aaggtggcga atttgtcact aaagatatct ctattgtatt
    36001 aaatacgtca tttaacaatg cagaagcgtt gaaaataaac tacggggatg catacccaga
    36061 acggacatca ccagatgaag aatttccagt agatgtgatt ggtcagtcag aaccagtgaa
    36121 agtcgatgaa cgctacttgt cagaaattat ttcagctcga atggaacaga ttttcaacaa
    36181 agcaaaagat gcattggacc aaatcgaagc attagagttg ccaggtggcg tcgtattgac
    36241 aggcggagct gctagtcttc ctggtgttgt ggacttggca caagaaatct tcggcgtaaa
    36301 tgtaaaattg tatgttccaa atcacatggg gctgagaaac ccggtattta caaatgtaat
    36361 aagtatcgtg gattattcag caaacttgag tgaagtgtat caattggcaa aaattgctgt
    36421 tacgggtgaa acctcagcag ctcgtcaaat ggtagttgaa caagaaacaa caaacacata
    36481 tgagagctat gaagcacctg aagaaacaat ctacgatgag ccggaaccaa aagagtccgg
    36541 agaaaatgta aaaaataaaa tcaagggttt ctttacaaat atttttgatt aattcatcct
    36601 gggaggaata agatatggaa ttctcaattg ataataatat caatgacggc gcagtaatca
    36661 aagtcatcgg agtcggtggt ggcggtggaa atgctgtaaa ccgaatgatt gaagaaaacg
    36721 ttaaaggtgt tgaattcatt acagccaaca cagacgtgca agcattaaaa aattcaaaag
    36781 ctgaaacagt tatccaatta ggccccaaat atactcgtgg tttaggtgct ggttctcagc
    36841 cagaagttgg acaaaaagcg gcagaagaaa gtgaacaatc cttacgcgaa gctttagacg
    36901 gtgcagatat gattttcatc actgcaggaa tgggtggagg aaccggtaca ggtgctgcac
    36961 caatcgttgc aggtatcgcg aaagaattgg gcgcattgac tgttggtgtc gtgacacgtc
    37021 cattcacttt tgaaggaccg aaacgtggcc gttttgctgc agaaggtatc gctcgtttga
    37081 aagaaaatgt ggatacatta ttaattatct caaataaccg tttattagaa gttgtagata
    37141 aaaagacacc gatgcttgaa gctttccgtg aagcagataa tgttttacga caaggtgtac
    37201 aaggtatctc tgatttgatc acagcgccag gttacgttaa ccttgacttt gctgacgtga
    37261 aaacagtgat ggaaaaccaa ggtacagcat tgatggggat cggtgtggct agtggagaag
    37321 atcgcgtagt agaagcaacg aaaaaagcaa tctcctctcc attgcttgaa acatctattg
    37381 atggagctga acaagtactt cttaacatca caggcggttt ggatatgaca ttgttcgaag
    37441 cacaagatgc ttcagatatc gtagcaaatg cagcaactgg cgatgtaaat atcattctag
    37501 gtacatctat caatgaagaa atgggcgatg aaatccgtgt gacggtcatc gcaacaggta
    37561 tcgatgaatc aaaaaaggaa cgcaagtcta gccgaccagc tagacaagca caaatgcaat
    37621 cacctgctca aaagactgta ttagatatgg atcaagctaa accaacttct tcagaagaag
    37681 aaaacagttt tggcgattgg gatatcagac gtgaacaaaa tgttcgtcct agagttgatg
    37741 attccaattt tgacaatatc gaaaaaaaag aatttgatac attcaaccgc gaagaaacaa
    37801 aatctcatgg cgatgatgaa ttaaatacgc caccgttttt ccgtcgtaaa aaataaagga
    37861 gaggaagagc acatgattgc tgacaacttg cagaaaatca agcaagaaat tcaggattcg
    37921 tgtgctcttg ttcatcgtga tccgaaagaa gttacattag tagctgtaac aaaatctgtt
    37981 gaatccgctc aagctgaaga attggttaga gctggcgtta tgaattgtgc agaaaaccga
    38041 gtagataaat tattagaaaa gaaacaagat ttatttgatt ttcctgaaat aaagtggcat
    38101 ttaattggta atttacagcg cagaaaggta aaattgattg taaatgaaat tgattacttt
    38161 catgcgttgg acagtttaag attagcagaa gaaatccaga agcggttaga gaaagaatta
    38221 tcctgtttta ttgaagtaaa tgtttctggt gaggaatcaa aacatggaat ttcaccagat
    38281 gagcttttac cgtttgtgga atctttagct gcctgcacaa aaatcaaggt tgttgggttg
    38341 atgactatgg cgcctaaaga agcttctgat gagcaaatcc ataaagtgtt tggtacgctg
    38401 aaagaattgc gtgatcttgt tcaagcaaaa ggttatgccc atgcaccatg tagtgaatta
    38461 agtatgggga tgagtcaaga ctttcaaatt gcgattgaag aaggtgcaac atttatccgt
    38521 gtaggaacag cgctattcaa agacgaataa aggaggaaca aaaatgccgc ttttaaacag
    38581 aggagcaatt gctaattttt tcggattagc tgatgaggaa gagtacgaat accaagatta
    38641 tcctgaaaca acaaaaaagg ctgtacagcc aactaaaaaa aatacaacac agaatcaatc
    38701 aactcattct gtatctgctg cgaatactca tgaaaaacaa aaaacggcga cttcacgtcc
    38761 tgtaacgcat gaaagtattc agccggctga aaagaaaaaa gtaattgaac tacatcaatc
    38821 acctacaaaa actgtagcga aagaaagaac agcttcaaga acctctacac aacctaaagc
    38881 aaatcttgct ggaaaaatca ctgtaatgga accaagagct tattctgaag ctatggcgat
    38941 tgctaaacga atcattgctg gagaagcaac cttagtgaat tttcatttag tagaagaaaa
    39001 acaagcgaga agaatcgttg attttctaac aggaacagtt tatgcactag atggagatat
    39061 caaacgtgtt ggagatgaga tttttctttg tacaccgact gggatggaga ttgacagttc
    39121 aaccgctcaa tcttttgcag atgcgaattt gtttgatctg taagaggagg aactacgatt
    39181 tatactattg cactattact tcatttactt caacgggcag cctatttcta tagcattatc
    39241 ttgatggtat atgcattgct ctcttggttt ccaggcgggt atcaatcagg gctaggacga
    39301 ttattagcta agatctgcga accgtatatc agtttgttcg atcgtctgcc tttgaggatt
    39361 ggacccttgg atttcacaat tttaggggca atcctgatct tgaacttagc cgtacaaggt
    39421 ttagcacaga tcgttatatg gataactgtc catttagttt attgattgaa gcaacaaaat
    39481 ctttattctc agaatagata ggaggggaaa attttggatg caaatgtata ccagcacttt
    39541 cgggcagatg aacgtacatt tatcgattcg gtaggcgatt ggattgaaca ggttcaagga
    39601 caatatgcac cctatctgac cgaatttctt gacccaagac aatcctatat tttagaaaca
    39661 ctgattcgac aaagcagtga tttgcgtttc atgttttatg gcggatatga acaggcagaa
    39721 agaaaaagat gtttgatttt ccccgattat tacgaacctg tagaggaaga ttttgaaaca
    39781 gcattgtatg aaatccatta tcctagcaaa tttgccagcc taagtcatgg gaaaatcctt
    39841 ggaactttag tgggaacggg gatcaaacgc tcttactttg gcgatatcat ctcggacggt
    39901 gatcgctggc aagtatttat tgcaaatgag gtggatcatt ttgttgctgc acaagtaaca
    39961 aagattggaa aggttgctgt acgcttggaa ccaatcaaat acacagaaat gattatgccg
    40021 aaagatagct ggacacaaga aagaggaaca gttagttcca tgcgactaga cagtgtgatc
    40081 tctgagattt ataatatttc cagacagcgg tccaagcagc tgatcgaagc tggtaaagta
    40141 aaagtgaact ggacagaaaa tacaagacct gattttgtac ttgaactatt agatattatt
    40201 tcagtcagag gttatggcag agtccagatc caagaacttg aggggaagac gaaaaaagaa
    40261 aagtacaggg tactgttcgg tgtattacgt aaataaaata aaaaggtggt ttactcatgg
    40321 ctttaactcc attagatatc caaaataaga cattcccgac aaaaatgcgg ggatataatc
    40381 aagacgaagt agatgatttc ttagatttag ttgttcgtga ctatgaggaa ttgactcaaa
    40441 gaaatcgaga attagaaaaa gcagtcaaac attctgaaga aaaacttgag tacttcaatg
    40501 aattaaaaga tgctttgaac caatcaatca tcgtagctca agatacggct gataaagtaa
    40561 aaacaagtgc aagcaaggaa tccgaagtga tcgttacttc tgcacaaaac aaagcagatg
    40621 agttagttgc gaacgctgaa aaacgtgcac atcaattaac aactgatgca gaagaaaaag
    40681 caagaaaaat cttgacagat gcaacagaaa aagcaagaca gcttgcgaca gaaactgaag
    40741 atcttaagaa aaaaacacga gtattccatc aacgtatcag cttgatgctt gagtctcaat
    40801 tagaacaagt gaaaagtccg gaatgggatg aaattttaca accattctcc agctacgtga
    40861 ctgacagcca tgaagtgatc aaagaagttt tagcaaaaca gcttgacaat gaaaatgata
    40921 ctgatgtaaa ctcagacaca ggagtcatag aagcacctat gacggcattt gatacacagg
    40981 ctattgatat ctcgatcgtg cctggtgaaa atgaaaacga gtagaacaga aaagcacttt
    41041 gttattctta aagcgagccg atgagagtgg aagttcggta agacactgca tggtgaaaga
    41101 tcctctactt gagacctgct tttccgaaat tagagtaaga agcagcggct gacctcgtta
    41161 ttggtcacag agagtttgca ctagatagat gcaactaaat ttgggtggta ccacggcatg
    41221 ttcgtccctt gcggctgaac atgctttttt gtgtccggaa aacaaataaa ggagtgtatg
    41281 gttgaatgaa aatgaaagaa accttgcaat taggaaaaac aaaattccct atgcgtggta
    41341 atttgccaaa ccgagaaggc gagtggcaaa aagaatggga agaaaatcga atctaccaaa
    41401 aaagacaaga gttgaacgaa ggaaaaccta gctttgttct tcatgatgga cctccatatg
    41461 ccaatggtaa tatccattta ggacattcat tgaataaaat cagtaaagac attatcattc
    41521 gttcaaaatc aatgtctggc tatcgtgcac catatgttcc aggatgggat acacatggat
    41581 tgccgattga acaagtattg gctaataaag gcgtgaaacg taaagaaatg tccatggcag
    41641 aatacttgga aaaatgtcgt gaatatgcac tttcccaagt agataagcaa cgagcagatt
    41701 tcaaacgcct aggagttgca ggagattggg aacatcctta tgttacatta gatccttctt
    41761 atgaagctgc acaagtacgc gtatttggta aaatggctga aaaaagctat atttacaaag
    41821 gattgaaacc aatttactgg tctccgtcaa gtgagtcttc tttagcggaa gctgaaattg
    41881 aatataaaga tgtaaaatct ccatctattt ttgtagcttt caatgtcaaa gacggcaaag
    41941 atattcttga tgaagataca gcttttgtca tttggacgac aacaccatgg acattgccag
    42001 caaaccaagg tattgctgta aatccaaaat ttacgtatgt attggtggaa gccgatggcc
    42061 gtaagtttgt cgtagcgaaa gatttgctag aaacagtcca atcagaaatc ggttgggaaa
    42121 atgtaacagt tctaaaagaa tttgctggac aagaaatgga atatatgact gcaagtcatc
    42181 cattctatga acgtgaatca cttgtgattt taggcgatca tgttacttta gacgctggta
    42241 ctggattagt ccatactgct ccaggacatg gggaagatga ctatatcgct ggtagcaaat
    42301 ataaactatc tgtagtgtca cctgtagact caaaaggatt gtttacagaa gaagcacctg
    42361 gatttgaagg tatcttttat gacaaagcaa atcctatgat cactgatcta ttaaaagaaa
    42421 aaggagcttt gctgaagcta gatttcttta ctcatagtta tcctcacgat tggcggacga
    42481 aaaagcctgt gatctatcgg gcaactccac aatggtttgc atctatcgat aaattccgtc
    42541 agaatatttt ggatgaagta gaaaaagtag agtgggttat cccatggggt aaaacacgtt
    42601 tgtataatat gatccgtgac cgcggcgatt gggtaatttc ccgtcagcgt gcatggggag
    42661 taccacttcc gattttctat gctgaaaatg gagaagcaat catcacacca gaaacaatcg
    42721 aacatgtggc aaaattattt gctgaacatg gatcaattat ctggtttgaa aaagaagcga
    42781 aagaacttct tcctgaagga tttacccacc caggttctcc taatggcgaa tttacaaaag
    42841 aaaaagatat catggatgtc tggtttgatt ctggttcttc tcatgaagct gttttacgtc
    42901 agcgaccaga attatcattc ccagcagata tgtatttaga aggatcagac caatatcgtg
    42961 gctggttcaa ctcaagtatc acgactagtg tggctatcaa tggtgtagca ccatacaaat
    43021 cagttatttc tcaaggattt gctttagatg gtgaaggccg taagatgagt aaatctttag
    43081 gaaacatcat tgcaccagaa aaagtgatca aacaatttgg tgcagatatc cttcgcttat
    43141 gggtatctag tgtagattat gaagcagatg ttcgtgtttc tatggatatc ttaagtcaag
    43201 tggcagaagt atatcgtaaa atcagaaata cgatgcgttt cttattagcg aatacagaag
    43261 atttcgatcc taaaaaagat actgtttcgt acaatgattt acgttctgta gacaaatata
    43321 tgaccgttcg attgaaccaa gtaatcaaag aaatccgaga agaagggtac gacaaatata
    43381 acttcatgca tatttaccgt acagttatga acttcttgac ggttgatcta tcgtcattct
    43441 acctggattt tgctaaagat gtagtttata ttgaagcaga agatgattat caacgtcgtt
    43501 gcatgcaaac tgttttctat caaacagctg tagctttgac aaaattattg actcctatca
    43561 ttccgcatac agcagaagaa atctggtcat tcctgaaaga agaagaggag tacgttcagt
    43621 taagcgagtt tcctggttat gaagagtttg caaaccaaga agaattgatg gatacctgga
    43681 ctgctttcat ggacttccgt gataatgtct taaaagcatt ggaagaagca agaaacagca
    43741 aattgattgg taaatctttg gaagcaaaac taactgttta tccaaaacaa caagttcgtg
    43801 aaatgttaaa agctttggat gcagatattg cacaattatt gatcgtttca ccagactact
    43861 ttgaagttat ggcagcagat gaacaagtgc cagataacgc tatggtattt gatgatgttg
    43921 ctattacagt ggaaaaagca gacggggaaa cttgcgatcg ctgtcgccaa gttcgtaaag
    43981 atgtaggagt agatgaaaaa cttcctcatc tatgcggtcg ttgtgcaaaa atcgttgaag
    44041 acttctatcc tgaagctgta gctgaaggat tcgaagaaaa gtaaaaggga aaagggggtg
    44101 tgacatgttc tttgtcacac ccccttttgt tatcaatcgt ttaattttcc acgttcacga
    44161 taccagttat ctggttgcca gatccattcg aatccgtctt tttcaagtag ttcgaaagct
    44221 gctttaggtc ccattgatcc agctttgtag ttgggaaaat ccggttgtgt ttggtcccaa
    44281 gcttttcgaa tgacatccac gatatgccaa gattgagcga cttcttccca atgagagaag
    44341 ttcgttccat caccattcaa cgcatctaat agtaattttt cgtaagcttc tgggctattt
    44401 tcaaccattt ctgcactatt tctaaattcc aatttgattg gttccgtttc aaatccttgc
    44461 cctacttctt tcccattcaa ggataaagaa aatccttcag ttggctgaat atagatcgtt
    44521 agaacattag gtggaagtgt cgtatcgtca catggttcat cgacagatgt tttgaataca
    44581 ttaacaggaa cttgtttgaa tacgatgttg atccgagtcc ctttttcagt caaacgtttg
    44641 cctgtacgta catagaaagg aacacctgac caacggaagt tatcgatagt gaatttgcct
    44701 gcgacgaatg tttcagtctc tgattgagca tccactttgt cttcttcgcg atagcccttg
    44761 aattgtacac catccaattc accttgagca tattgcccgc gaacgaagtt ttctaacact
    44821 tcttcttcgt tataaatgcg gatagctttc aatgctttga tcttttctgt acggatttct
    44881 ttttcggaaa atgctactgg cggttccatt gcaagtaatg ctacaacctg taaaatatga
    44941 ttttgcacca tgtctttaag tgcgccacta tgatcgtagt agcccccgcg atcttctaca
    45001 ccgaggtttt cgccaaaagt aatttggata ttgtcgatat agcgattgtt ccattgagcc
    45061 tcaaaaatat tattggcaaa acggatagct gagatgtttt ggatcatttc ttttcctaaa
    45121 taatgatcaa tcctgaaaat atcttgttct gggaatactt ctctgatttg ctggttcaat
    45181 tcaaaagcag actggtaatc agaaccgaac ggtttttcta tgatcaaacg attgaagcca
    45241 ttttttgtca taatattttg tgatttcaaa tgagaaacga tcgttccaaa aaattgaggt
    45301 gccatagcca aataatagac acggttaccg tttaattgat agtgtccatc taatcggtca
    45361 gacaattctt ttaatgtctg ataatgcgtt gaatcattta catcatggga ttgatagtaa
    45421 aaatggcttg caaaggtagt tgcttcttca tctgaaggat tcaatgcacg gattgtttct
    45481 ttgacaactt cacggtagtg ctcatcgctc cagggacggc gagcggttcc gattacagca
    45541 aaatgctcgc ctaaatttcc ttttctgtaa aggcggaaca aagacgggta caatttacgc
    45601 tgggctaggt cacctgtacc gccgaatatc gtaaacaata catttttttc attagacatg
    45661 tggatacact cctttatatt ttatcgacaa aaatcgtgct ggccgcctta aagctgacgg
    45721 ggattttctt tctatcattt tggatagtga tcggtccttc ataagctgcg atgtcaacga
    45781 tctcatattc ttcgttgata ttcaggtcaa tagtgactaa gtaatccaac aattcttttt
    45841 catcaagcac tcgtgcgata cgcacttttg taccgacagg ataggactcc aatgtttgtc
    45901 ggcgttcttc atggacaact tcctcttttt taggaatcga tcctccatga ggacaatgtt
    45961 caggatgatt caaatagttt tctaaatgat ctgcaagatg ttccgaagtc acatgttcca
    46021 atacttcagc atcatcatgg acttcattcc agctatagtc taaatgttca actaaaaaaa
    46081 cttcccataa gcgatgtttg cgaatcagac tgctagcttt ttgcagtccg cttgcagtca
    46141 attgtacacc ttgataaggg gaatgttcta caagctcttc tttcacaagt ttagaaatca
    46201 tttcactgac agatgcagct gagacatcta gactagatac gatttgttta ttgttgactt
    46261 tcgttgtatc tccacctagt tctaaaatga ttttcaaata atcttctcga tttggtgtca
    46321 tattcgatca tcctttcatg gtaaaaaacc atcattagta agtgtaacaa aaaaaacgtt
    46381 aattgactat ttttctaaat aaaaaacaaa ggatttttag acgtttttac aacaaaaaac
    46441 gaaagatgat agagaagact cattctttcg tttataaagg atatatatat acattgggag
    46501 ggatctattt ttcagataac aacttatatg aagtgtctgc agattcgtca taaagagttt
    46561 gttcgggata agaaaagata agataaaaac catactgatc acgtacgact aaacgatatt
    46621 catctatttc taccacagat gtgccaagag actgaccgtt gatcaaaaac gtgtaatcag
    46681 atgtaatctg taattgttgt ttttgctggc tatcactaaa gaaccatgta ccttgatact
    46741 ttgagagtcc ttctgacttt tccgcttgtt ttttcttttt caagtaaata aaacttgaga
    46801 gaacggtaat gagtgaacca agaaaataga tcattttttt tatcaccaaa tcactccttt
    46861 atgagaaaat aatcgcatag acatattctt tcttctttat ttcattgtac cgaataaaag
    46921 ctaaaatttc tctaagactt tcgactggtt tttttactgt ttttctacaa attatgatag
    46981 gatacatagt aggaagaaag gtgacataat ttgaagtatc agcaagtgga aatagctttt
    47041 tttattgacc gcccgaatcg gtttatagca cattgtatga aaaaagacgg agaaataatt
    47101 attgcgcatg tcaaaaatac aggtagagga aaagaggtcc tgataccagg cgctgaagtg
    47161 gctattgtat ttgctcctgg acctaaacgg aaaaccgcct atgatctgat tgctgtaaaa
    47221 aagaagggtg actggtttaa tatcgatagt caattgccta atcggctggc aattgatggt
    47281 attttagacg gcacgatcct tttaccagga ttaactggtc catttacggt atacaagcgt
    47341 gaagtaactt atcttcactc caaatttgat atttatttag agacggtggt cggacaaaaa
    47401 gcatttgttg aagtcaaagg tatgacactt gaaaataaag cgattggtgc ttttcctgat
    47461 gcgcccacac tcagaggatt gaaacatata ggagaattaa cgtatgccgc acaagatggt
    47521 tatgccgctt atgtcttgtt tattgctcag ttcgaacatc ttcatctggc aacgatccat
    47581 gaagaaatgc aaccagcact agctgatatg gttcgacatg cgcagcaaag cggagttcaa
    47641 atcctcgcct ataattgtca agtatcggca gatgaggtaa ctgtcaagca ggccattcca
    47701 tttgatgtag atatcccttt tgaagatcct atacaataga aagaaggtaa aataatgatc
    47761 aacttaggta ttatcggaac aaattggatt actcatcaat ttgtccaagc agctttaagc
    47821 acaaaaaaat atgaattgac agcggtatat tctagacatt tggaaaaagc acaggaattt
    47881 ggttctcatt atgaagggga tatcgaattc gcaacagatt tgaagacttt tttcaatatt
    47941 actcatatgg acaccattta tattgcttct ccgaatagtc ttcactttag tcaagctaag
    48001 caagcaattt tggcaggaaa aaatgtgata gttgaaaagc cagctttttc gacgccaaaa
    48061 gaaatggatg aaatcatcca tttggcaaat gaacacaatg tgtatttttt tgaagcagca
    48121 agaaatatcc atgaatcagg ttttaaaaag attgccgatt acttaccatt aaaagacgag
    48181 ataatcggtg caaatttcag ctatatgaaa tattcttcgc gctatgacca agtattagat
    48241 ggagaagaac cgaatatctt ctcgactcat ttctcaggtg gtgcattgat ggatttaggt
    48301 gtttaccttg tatatgcagc tgttgcttgg tttggaatgc caaaagaagt ccattatttc
    48361 ccaagaaaga tttctactgg tgtagatggt cttggatggg ggatcttacg ttatgagaat
    48421 tttgatgtat cgatccagcc aggtaaaatc ggtgattctt atttaccgtc agagatttat
    48481 tttgactcag gaacgctagt gatgaatgga gtcaatgcaa ttgaacaagc acaatttcat
    48541 gatcgcgcgc ataaagaaat cattgatttg gcggtaacag ctgcagaaaa tccaatggca
    48601 gatgaagcag aggattttgc caatgtcatg gctcatccga aagatccagc ttggggattg
    48661 ttgtatgaag agtggatcga gctagctaga aatgtgaacc aagtgattta tgatttaaga
    48721 aaaaatggcg gcatcaaatt cgatgcagat aatgaggaag atttttaatg aacgaatttg
    48781 tacaagtatt tcccgaaaat tggcagaaaa aatatgagga taaagggttt atctcacctt
    48841 ctcttattca acgagcagtt tttcagccac taagtaataa acaaagtata gtagcggttt
    48901 ctcctacagg ttcaggtaaa acactggctt atctttggcc tctactacta aacgtggaac
    48961 caggtgaagc aagtgcgttg gtcatttttg cttcttctca agaactagct attcaagtag
    49021 cagatgttgc acgtgaatgg ggaaaagaca aagagttgaa agtccaatct ttggtgggcg
    49081 gagccaacgt caaacgtcag attgagggtt tgaaggaaaa accagaaatc ttgattggta
    49141 caccaggaag aatattggaa ttgatgaaag caaaaaaaat aaaagcacat caagtgaaaa
    49201 caatggtatt tgatgaagca gaccagctat ttgacgctgg gaacagccaa atcattgatc
    49261 aaattcttca tcaagctcca acagagtatc agctagcttt tttttcagcg acagcagatc
    49321 gttcccttga aagcattgaa aaaatcactg ggaaaacgtt tgagacaatt gacgtgacag
    49381 cagaggacga ttctcgaaaa ggactgagac attacttttt acgtgtaccg atacgaaaga
    49441 aagatgacta cttgagacgt ctcacgcata tagaagattt tcatggtctg attttcttta
    49501 atcaactgac tgaattaggc gtaatggaag aaaaattgct gtatagaggt gtaccggtag
    49561 gaagtctggc ctctgatcag aacaagctac tccgaaaagc agcgctagaa caattcaaaa
    49621 agaaaatggt cagagcactt ttgaccacag atgtggcggc tagaggttta gatattacag
    49681 gtcttcctta tgtagtgaat gcagaagttc cgttaagtga agaagcttat ttgcatcgtt
    49741 cgggaagaac tggtcgaatg ggtaatgaag gaatagtaat tacactcgta caagataata
    49801 atctaagaga tttaaaacgg cttttacgtc catcaaatat aacacttgaa gagatttttt
    49861 tatacggagg taagctgcaa actgagcagc caaccaaaga agatgcagct cctgcagtaa
    49921 ataaaaagta tgcgaaaact ttttctggaa aggagcagaa cccagaatat aacaaagaaa
    49981 aaataagaaa aaaagttaaa aataaatcaa aaaaagagag aaataaagga gcaaggcgca
    50041 aataagcgcc tttttatgct atgattatga aaacgggaac aaaattggtt gccagaggag
    50101 gggttatgtg aaagaacgtc aagaagagat cttgcagcta ttgacagaaa accaacaagg
    50161 attgacagca agtgatgtag ctgagcgctt aactatcgat cgaagcaatg caagccgata
    50221 tttaagtgaa ctatacaagg cccatcatat tgtaaaaacc gctggccgac cagtcgttta
    50281 ctctttgcca acggaaaagt caaaatctga tgaagtgcat gtcgactctt ctacgcaagt
    50341 gacttttgaa acactcgttg gagaaaatga ttctttaaaa gtcagcatac agcaggcaaa
    50401 agctgcgatt ctttatccac cgcgggggtt gcatacgatt atttttggag aaactggtac
    50461 agggaagtcg atgtttgctg aatgtatgta tcattttgca atcgattcag agatgctttc
    50521 tgcagatgcg ccatttgttt ctttcaattg tgcggattat gctcaaaatc cgcaactgct
    50581 ctttggacac atctttggga tcaaaaaagg ggcttacaca ggagcagctc aagatagtcc
    50641 cggtctaata gccaaagcag acggcggtat attgttttta gatgagatcc accgtttacc
    50701 gccggaaggg caggaaatgc tgtttacttt cattgataaa ggtatttacc gtccgcttgg
    50761 agaaagtgca caagtacatg aagcatctgt tcagatcatt ggggcgacga ctgaatcttc
    50821 agaaagtttc ttatctacgt tcaatcgacg gattcctatg gcaatcacct tgccgaatct
    50881 tgcttcacgt tcactggatg agcgttatga aatcatctct ctttttatca aacaagaagc
    50941 gaatcgtttg aatcaacgta tcgatatcga aaaagaagcg attcttgcct tcatgcttta
    51001 tgatgcggaa ggaaatatcg gtcaagtgaa acgagacttg aaactagtct gtgcaaaatc
    51061 ttttttacat taccgtactc atcatgagaa aaagctaatc attcgaaaag aagaactttc
    51121 ccttcaagta caaaaaggat tactgaaaat caaggaaata ccggaacgtc tcgaccgatt
    51181 catggacagc aaaagtcagt atttgacatt tgaaccagga tttgctgatg ttgtctggtc
    51241 acaagatcca gaacgaaaca tgcaggtcta taacgatatt gaggaaaaag tcacttctct
    51301 ttccgaaacc ggtgtggaga acattgatct agagaccctc atttcaaaag atgtggatgc
    51361 ttatttccag acatatgtca aagaattgac taaaagttcg attcccaaag atcttttgcc
    51421 agatgaaatc tggcggttaa ccaatcaatt gtatgatgtg gctgaaaaac ggttagaccg
    51481 tatttataat gaaaaagctc gttttgcttt tgctttgcat ctgcaaagta cattggatcg
    51541 tgtgagagaa ggtcatatga tcgtccaccc agatttgaat aacgtacgga aaaattttaa
    51601 gagggaattc caggtagcaa tcgatctttc aacgatcatt gaagaagaag aacaagttga
    51661 gattcctttt gatgaaatcg gctttattag tatgtttctt tctatcaatc taggagaaag
    51721 tgagcctgtt aaagaaaaca aagtagatgt tctcatattg atgcacggca gacagactgc
    51781 ttcaagcatg ttggagatgg cacaagaact attaggaaca aaagtaggga aagcttttaa
    51841 tatgccactg gttatggaag ttcaaacaat gtatgaagaa gtgttacaat atgtcaaaaa
    51901 ctatcaagag catttgacga atggcctact cttgttaaca gatatgggat cattaaacac
    51961 cttcagtgat ttgatttatg aagagactgg cattcggacg aaagctatca cgatgacaag
    52021 taccatgatt gtgatagaag cgatacggat ggcaagtgtt ggaagaagct tagaagatat
    52081 ttatcaaaat atccagcttt cctttgaatc gatcgtacgt gatcagttca aagcggaaaa
    52141 gtctgaaact ttacagcgta aaaaagccgt aatcgtgaca tgctttactg gtgaaggagt
    52201 agctgccaaa ttatatcagc gtattatgcc agtgattaat caaaataagg tagaactgat
    52261 ccagatgcag tttttagaac gtgaatcatt caaaaaacac atcgatgcct tattggaaga
    52321 atatgagatc aaagcaattg cgggaacagt tgatgttgat tatcaaaata tcccattttt
    52381 ttcagcctat gacgtttttg atgatgaaaa actaaatatt ttaaagcgta tagctgatga
    52441 tgagatcccg acagctaaaa ttgtcgattc acttgctgga tcattaagcg atgtcttttc
    52501 tattgatgaa ctgatccgtt cattgcaaaa gacagttcag cagatccaga ccgatttgtc
    52561 gattatcgtg gaacctggag tagatactgg aatcgttatc catttagcct tcatgttaga
    52621 cggattgatc aaacaagaaa gccatcggca atttgagaaa ctgtctattt tctcaaaaat
    52681 ttatcgtttg gaaatggata tcgttcgggc aaacttgatg gcactagaac gaaaatatca
    52741 ggtaaaaatt ccagaagatg atatcgcatt tcttacacaa atgtttcttg aaaataaaat
    52801 aaaaaaatga acacactaat catactgttt acacactaaa catctgtgtg cgcactataa
    52861 aaaagagcta aaaagcatga aaacgctatt tcagctcttt tttttattgg catgcttctt
    52921 gcgttatata aaagtgtaaa aaaataaaag gagtgttaaa aatggcaaaa aaaacaatca
    52981 tgttagtttg ttcagcaggg atgagtacga gtttattggt cacaaaaatg caaaaagcag
    53041 cagaagaaaa agggatggaa gccgatattt ttgcggtctc tgcctcagat gcagataata
    53101 atttagaatc taagaatgtc gatgtattat tattaggtcc tcaagtacgt ttcatgaaag
    53161 ctcaatttga acaaaaactt gcgcctaaag gtattccatt agatgtcatc aatatgcaag
    53221 actatggcat gatgaacgga gaaaaagttc ttgcacaagc agaaaatcta atgaaataaa
    53281 tcaagtatgc agttgggggt ataacaacgt ggaagatcaa caaaacctag aagccatcat
    53341 ggggttaatc atgtttggcg gtaatgcaaa gagcgatgca atggaagcga ttgcggctgc
    53401 taaaatcggt gattttgagt tagcggatag gaaaatcgct gatgcagaag aatcacttat
    53461 acaagcgcat catgcacaaa cagacatgtt gacacaagaa gcacaaggaa atcatatgca
    53521 agtgacatta ttgactgttc acagtcaaga tcacttaatg acatctattg cgtttactga
    53581 tttagcaaaa gaaatcatcg aactttatcg tcgcgtatac agcgaataaa tacaacttct
    53641 tttttgaacg gaagaagaaa aatttttgaa cggatttttt attttgttat aaggagaact
    53701 ctctttatat aaaaaactta taggaggaag taaaaaatga acggactatc aagttggatg
    53761 gagaaataca tcctgccggt tgccgcaaaa atcggagcgc aaaaacattt agttgctttg
    53821 cgtgatgctt ttatcggcac actgcccgca acaatggcgg gatcagtcgc tgtaatgatc
    53881 aatgcaatta ttcgtgattt accgcaacag tttaacagta cctatgctga cagtttagca
    53941 gcagacaaag gaatctttgc cgtattgaat gtcatcatcg gaatcaatgg ttttgtctgg
    54001 aacggtacac tagcaatcgc cggattgatc tttgcctttt cttgggggta taatcttgcc
    54061 cgagcttatg gagtaaatga tctagctggg gggatcgttg gtttagctac cttgattcaa
    54121 ggtatttcct ttggtccagc attatcagca gcattagatg tgaatgttcc ttcaaatgtc
    54181 gcagatgcaa tcaatagttc tgagattggt gcaacgattg cagatggcac actgtcagtt
    54241 ggattatggg gttggctaaa actagatcat ttgaatggaa acgcttattt tacagttatg
    54301 atcatgggag ctatttcagt cattattttt tgtaaattga tgcttgccaa cattacgatc
    54361 aaaatgcctg attcagtacc accagcagta tctaaagcat ttgcagccat tttaccagcg
    54421 acgatcgctt tatacgtcat tgctattatc aattatatcg taggtcgtgt agctgatggg
    54481 caactgatca ttgatttggt gcaaaaatat attgcagaac cattcctagg attatctcaa
    54541 gggttgggag cagtattgat cgtcacagta tttgttcaag tattctggtt ctttggtatt
    54601 catggaccaa atgtattggc accagtgcta gagggaatct ggggacaagc acaactaatc
    54661 aatattgata ttttccaaaa aggttataac ggaaaaactg gtacaccagc cgtgttagat
    54721 gcaatcgaag atggaaaagc ttatatgtgg gtccgcggat cgttcgatgc ttttgcatgg
    54781 tttggcggat caggcggaac gatcgtctta ctgatagcta tcttgatttt ctctaaacgt
    54841 gcagattatc taacagtagc taaattatcc attgggcctg gtatcttcaa tatcaacgaa
    54901 ccagtgatgt ttggtctgcc aatcgtattg aatgcaatca tgtttatccc attcttgatt
    54961 gctccagtag tagcaacagc aattggatgg acagctactt atcttggcct agtcgctccg
    55021 gtatcgcagc aagtcacatg ggtagtaccg ccgttcttat tgtcattctt agcaacagga
    55081 gcggattggc gggcaccgat cgtaacatta gtatgtatgg tcgtgacttt cttgatttgg
    55141 gcaccatttg tcatctctgc aaacaaaatg gaccctgccg caggcgaaca acaataagat
    55201 gaacgtgagg agcgggacgc tttaagtttc gttcctcata atgaattttc gaggaggaac
    55261 tgaaatgatt acaattgcca gtgcaatgca acaagatgaa atcaaagaat tgttagaagg
    55321 atacaatgaa gaaggaatga cttttacatt taaagaaaaa caaggaatca agctgttctt
    55381 tgaaacaagt gcagaagatc cggaagttgc ggctaaaaaa gcaaaggaac taatcaaagc
    55441 acagccttgg ggaacagtcc tttattttca atcaacagct gtttagaaaa tggtttaaaa
    55501 agataggatg gttgagatga taaaaaacgg tttattttta tgtagtattg gttctttgat
    55561 cgtgttgtat agtttgaatc cacattcgca aaggtatgat ttgtatagta tggtcacagg
    55621 tctttttcta acaggattag gaatctattt cttcatgaag gggaaaaaaa gagaagaagc
    55681 aaaggagaaa aataactaat ggaaatcacg aaaaaattaa atattccagc atcgtttttt
    55741 tacgataaag ttatggactc tgtccttttc gatgtacgta aagcgaccgg caaatcagta
    55801 actagaaaac agctgaaaaa tttggagtac gtcaaacaat tctcgcaaaa taaccgggct
    55861 cgcataaaaa tcgaagaagt cattgaaaac cagtcttata agtttcgtac atcgacaacg
    55921 aagaatgaat tcgtcgttca ttatcaaatc aaaccattgg acgattcgac ctgtgaggtt
    55981 cattacacag aaaaaatgga gtcttatggc atgcttcaaa agttgaatga tatgctttta
    56041 ggaacaatct tgatgtattt taaacggaga cgtttcaaaa aaatgctaga tatgatcgaa
    56101 gcaacgtatt aaatcaatga aacatggtac tatttagtta ttgaaaaatg aagtaagcta
    56161 gaaggaattt ttcataggaa gatagatctt tcctgcgaat cctcactagc ttttttgtag
    56221 gaaggggcag caaaatgaca aacaaagtaa gagagctagg tgtatcttcg attgaggaaa
    56281 tgtttgattt agccacttat gcatttaatg gggcagatac acctgaaagg cgagaacgtt
    56341 ttcgtacact tgcggagaat tcttggaatt acggattttt tgatgaggag gatcggctaa
    56401 caagtcaagt catggcgacc ccttttccag tgaattttta cggacaagag tacctaatgg
    56461 ccggcattgg ctatgttgct tcttatccag aagcaagggg acaaggcggg atcaatcaga
    56521 tcatggaaaa gatccttgaa gattgccgtg atcgtaaagt aagcttatct tatctggcgc
    56581 cattctccta tcccttctat cggcgctatg gctatgaaca atcatttgat aaaatcagtt
    56641 atcaactaaa cagtcgtgat gtgccgttta tcaaaaaaaa atctggaaaa ataaagcgta
    56701 tggattttga agatgcaaaa gctgcaatca gaagaattta tgaacagatg ccggaaaacc
    56761 aacgagctgg tctgaagcgc gaagactggt ggtatacgta taaattcaag caaaaaggca
    56821 attaccagtt tgctttgcat tttgatgata atgaggaagc aacgggatat atcgtttatc
    56881 agcttgcaac gtcttccttt attattgcgg agtggggatt tttggatcat gatgcttttt
    56941 gcagccttgt tcgcttcgta agttctcaca atggtgcttt tgaaacattt gaatattcat
    57001 gcggctacgg cggtaatgat caaaattatc tattagaaaa tccatttgct tctgtaatga
    57061 tcacacctta tatgatggcg aggatcgtag atatttcttt gtttttggaa aattatccat
    57121 ttaaaaagag acaagctgca tttgccctac aaatagaaga agatcattat gcgaaatgga
    57181 atgaaggaat cgtggaagta acaatcgaaa atggaaaaaa tcatctctca aaagtagaac
    57241 aaacaaaact cccaatcgtt cgaggaagta tccaacatat cacgcagcta ttattagggt
    57301 atcgtaaagt ggaagagttg gtattccaag aaagaataca ggtgacaaaa gaatatgaag
    57361 aaacgttaag ctcaatcttg ccacagcaac agccaattct taatgactat ttttgataaa
    57421 aaacgggtga tgacaatatt tttgtcacca cccttttgtt tttcatttgg catttgaagg
    57481 aaaatgttaa agtaaatcct cttttttcgt aaatttgaat tttttgtttt cttttgtttt
    57541 agggttttct tttttgtggt cttggattgc ttctttcgta atcttttccc gatttttttt
    57601 agatgaatct aagcgttctg cgtatacttt ttcttttggc ttatcattca ttggaaagga
    57661 cctccctaca ttatttcatt ctacctctag tttatcatct gttaaggact agtggaaacg
    57721 ttttgcctaa cttataagct gattttttta gcagataagc ttgcaagtaa aaaacaaaca
    57781 agctacaatt gatttattga aattgttcct gtagctcagt aggatagagc aatcgcctcc
    57841 taagcgatag gtcgctggtt caattccagt caggaacgta caaataaccc ctgtatcctt
    57901 tgcggtacag ggggttattt tgtataagta gacattgtag tttctaaact gctatgtcct
    57961 aaacgtttcg atacgctaag tatatttact ccttgataaa gtaaaataga tgcatgcgta
    58021 tgtcttagtc cctcaataaa cagtcgaaga gaaaataggc aaaaattgat tactaaagaa
    58081 aaacttacta ggtcgacggt gtagttgatt ttcatacttc aacagattcg aattaaacga
    58141 tttcgattgg cttataccag tcctatccga cgaaagatat atcgttctta actctagcgg
    58201 tataacttga agctttacgt aaagccaatg ggaatgaact ggagatgact ttttattatt
    58261 tttagtaata gctgatgggt taatacttac aactttcctt ttcaaagtac catataaaaa
    58321 aacggtgggg ttgtttagcg aaataaagga tctaagccaa tagcttagat cctttattct
    58381 atgctttata ttattttttg catgtgtgcg aaagtatact caagcccaat cgccatttcg
    58441 gaaaatcggt actgtagagc catcttcacg gattccgtcg atatccatct tgtcagatcc
    58501 taccatgaaa tctacgtggg tttgacttct attcaatcca gcttctgcta attcttcatc
    58561 ggacatttct gtaccgcctt tcacactgaa ggcataagca gaacctagtg ctaagtgatt
    58621 cgatgcgttt tcatcaaata atgtattgaa aaagatgatg ccagattgag aaataggtga
    58681 agggtcaggt acaagtgcga cttctcctaa acgacgcgca ccttcatctg tatcaagcag
    58741 ttttgctaag acatcttctc cttgttcggc agaaaaatca atgacttttc catctttaaa
    58801 agtaaatttc atacccgaga tgatggtgcc agcataactc agtggctttg tacttgaaat
    58861 gtaaccatct actcgatgac tgtcaggtgc tgtgaacact tcttccgtcg gcatatttgc
    58921 catgaatttt tctccacgag cattatagct tccagcgcct tcccaaagat ggtttttagg
    58981 caatccgata atgatatctg taccgggagc agtatagtgc agggcagaaa attgttctcg
    59041 attcaattct tctgcttttt ttgctaattt ttcatcatgc ttcttccatg ctaagacagg
    59101 gtcttcttca tagacacgag tcgttttgaa aatctgatcc cataatgcct caacttgttt
    59161 ttcttctggc agttctggga agacttttgc tgcccattgt ttaccagccg cagccacgac
    59221 agtccaactg actttatttg cttgagttgc cttgcgtaaa ttcattagtg ctttgccagc
    59281 cgctgattga taagacggct actcgatggc tatctacacc agcaaatgca tcgggatcgg
    59341 cagaaacaac actgatcctg cttgctcctt tttcaagcca ttcatccgct tgatcgattt
    59401 tagattgtgg tacattctca atacgatccg ttgcagcatg taaaagaaat tctctttgga
    59461 tctggtcgtc agtccattga acgataacct cagcggctcc tagtttataa gcttcttgtg
    59521 tgatcaatcg agcaaggggt gcctgttcca cgctgatctg taaaacaact gtgtgtcctt
    59581 tttctgtggc tacgccagtt tctgcaatta agcgtgcata tttttttaga agttcattaa
    59641 aatcagataa cataataaaa attcctcctc accatttttt ctggtaacta ttagcatgat
    59701 accatttaat tatgattcaa acaatgaggc ctttaaataa aaaaaagagg ttattttttt
    59761 aaggttaagt ttcatgatat atgttactac ttgctatttc agcttgtgaa gaaaatcaaa
    59821 aactaatttt gcatatcaaa aaaaacatgt tacaatgaaa ttaatcaatg gtggaagcgt
    59881 tataaaaaaa ttaaggagat taaaaaaata tggcaagaaa aaagacgatt actagagaac
    59941 agattttggc agctgcttat gaagtagctg ccaccgatgg atttacacga tttactgcga
    60001 gaaacatcgc gaacaaaatg aattgttcca cgcaaccgat ttatcttgag tttaaaaata
    60061 tggatgactt gagacaagct ctttttgagc agatctatga tcatttacaa tatgaagtgt
    60121 tcactgttga gcatacaggg aatacgatta tcgatcttgc ttttaatt
//
LOCUS       DAZABD010000009        59668 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000009 DAZABD010000000
VERSION     DAZABD010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 59668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59668
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.009"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(451..1761)
                     /locus_tag="ACHALG_000629"
     CDS             complement(451..1761)
                     /locus_tag="ACHALG_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HFN2586065.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1773..3191)
                     /locus_tag="ACHALG_000630"
     CDS             complement(1773..3191)
                     /locus_tag="ACHALG_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFN2586066.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3431..4999)
                     /locus_tag="ACHALG_000631"
     CDS             complement(3431..4999)
                     /locus_tag="ACHALG_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HFN2586067.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(5003..5413)
                     /locus_tag="ACHALG_000632"
     CDS             complement(5003..5413)
                     /locus_tag="ACHALG_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFN2586068.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5410..6333)
                     /locus_tag="ACHALG_000633"
     CDS             complement(5410..6333)
                     /locus_tag="ACHALG_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HFN2586069.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6330..7208)
                     /locus_tag="ACHALG_000634"
     CDS             complement(6330..7208)
                     /locus_tag="ACHALG_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HFN2586070.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(7195..8532)
                     /locus_tag="ACHALG_000635"
     CDS             complement(7195..8532)
                     /locus_tag="ACHALG_000635"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HFN2586071.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8535..9638)
                     /locus_tag="ACHALG_000636"
     CDS             complement(8535..9638)
                     /locus_tag="ACHALG_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFN2586072.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9712..11220)
                     /locus_tag="ACHALG_000637"
     CDS             complement(9712..11220)
                     /locus_tag="ACHALG_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFN2586073.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(11186..11878)
                     /locus_tag="ACHALG_000638"
     CDS             complement(11186..11878)
                     /locus_tag="ACHALG_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFN2586074.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11900..12874)
                     /locus_tag="ACHALG_000639"
     CDS             complement(11900..12874)
                     /locus_tag="ACHALG_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN2586075.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            13174..14046
                     /locus_tag="ACHALG_000640"
     CDS             13174..14046
                     /locus_tag="ACHALG_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN2586076.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14504..16702)
                     /locus_tag="ACHALG_000641"
     CDS             complement(14504..16702)
                     /locus_tag="ACHALG_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN2586077.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16934..17926
                     /locus_tag="ACHALG_000642"
     CDS             16934..17926
                     /locus_tag="ACHALG_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN2586078.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17986..19407
                     /locus_tag="ACHALG_000643"
     CDS             17986..19407
                     /locus_tag="ACHALG_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFN2586079.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19400..20899
                     /locus_tag="ACHALG_000644"
     CDS             19400..20899
                     /locus_tag="ACHALG_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFN2586080.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20938..22332
                     /gene="uxaC"
                     /locus_tag="ACHALG_000645"
     CDS             20938..22332
                     /gene="uxaC"
                     /locus_tag="ACHALG_000645"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFN2586081.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22491..23174
                     /locus_tag="ACHALG_000646"
     CDS             22491..23174
                     /locus_tag="ACHALG_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HFN2586082.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            23176..24012
                     /locus_tag="ACHALG_000647"
     CDS             23176..24012
                     /locus_tag="ACHALG_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFN2586083.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            24023..25777
                     /locus_tag="ACHALG_000648"
     CDS             24023..25777
                     /locus_tag="ACHALG_000648"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN2586084.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(26053..26394)
                     /locus_tag="ACHALG_000649"
     CDS             complement(26053..26394)
                     /locus_tag="ACHALG_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFN2586085.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(26462..28633)
                     /locus_tag="ACHALG_000650"
     CDS             complement(26462..28633)
                     /locus_tag="ACHALG_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFN2586086.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            28844..29701
                     /locus_tag="ACHALG_000651"
     CDS             28844..29701
                     /locus_tag="ACHALG_000651"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFN2586087.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(29772..30629)
                     /locus_tag="ACHALG_000652"
     CDS             complement(29772..30629)
                     /locus_tag="ACHALG_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFN2586088.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(30614..33316)
                     /locus_tag="ACHALG_000653"
     CDS             complement(30614..33316)
                     /locus_tag="ACHALG_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFN2586089.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(33329..34618)
                     /locus_tag="ACHALG_000654"
     CDS             complement(33329..34618)
                     /locus_tag="ACHALG_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HFN2586090.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            34768..35814
                     /locus_tag="ACHALG_000655"
     CDS             34768..35814
                     /locus_tag="ACHALG_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFN2586091.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            35816..37969
                     /locus_tag="ACHALG_000656"
     CDS             35816..37969
                     /locus_tag="ACHALG_000656"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HFN2586092.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(38442..39893)
                     /locus_tag="ACHALG_000657"
     CDS             complement(38442..39893)
                     /locus_tag="ACHALG_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFN2586093.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(39890..41614)
                     /locus_tag="ACHALG_000658"
     CDS             complement(39890..41614)
                     /locus_tag="ACHALG_000658"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFN2586094.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            complement(41607..42221)
                     /locus_tag="ACHALG_000659"
     CDS             complement(41607..42221)
                     /locus_tag="ACHALG_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFN2586095.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            complement(42566..44023)
                     /locus_tag="ACHALG_000660"
     CDS             complement(42566..44023)
                     /locus_tag="ACHALG_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN2586096.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            complement(44064..44984)
                     /locus_tag="ACHALG_000661"
     CDS             complement(44064..44984)
                     /locus_tag="ACHALG_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN2586097.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            complement(44996..45943)
                     /locus_tag="ACHALG_000662"
     CDS             complement(44996..45943)
                     /locus_tag="ACHALG_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN2586098.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            complement(46422..47141)
                     /locus_tag="ACHALG_000663"
     CDS             complement(46422..47141)
                     /locus_tag="ACHALG_000663"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFN2586099.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(47190..48836)
                     /locus_tag="ACHALG_000664"
     CDS             complement(47190..48836)
                     /locus_tag="ACHALG_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HFN2586100.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            49015..49605
                     /locus_tag="ACHALG_000665"
     CDS             49015..49605
                     /locus_tag="ACHALG_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFN2586101.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            49697..51202
                     /locus_tag="ACHALG_000666"
     CDS             49697..51202
                     /locus_tag="ACHALG_000666"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HFN2586102.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(51286..52638)
                     /gene="celB"
                     /locus_tag="ACHALG_000667"
     CDS             complement(51286..52638)
                     /gene="celB"
                     /locus_tag="ACHALG_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFN2586103.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNSSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(52638..53156)
                     /locus_tag="ACHALG_000668"
     CDS             complement(52638..53156)
                     /locus_tag="ACHALG_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2586104.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(53172..53498)
                     /locus_tag="ACHALG_000669"
     CDS             complement(53172..53498)
                     /locus_tag="ACHALG_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HFN2586105.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(53511..55490)
                     /locus_tag="ACHALG_000670"
     CDS             complement(53511..55490)
                     /locus_tag="ACHALG_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFN2586106.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(55511..55825)
                     /locus_tag="ACHALG_000671"
     CDS             complement(55511..55825)
                     /locus_tag="ACHALG_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HFN2586107.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            55993..56286
                     /locus_tag="ACHALG_000672"
     CDS             55993..56286
                     /locus_tag="ACHALG_000672"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HFN2586108.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(56364..57476)
                     /locus_tag="ACHALG_000673"
     CDS             complement(56364..57476)
                     /locus_tag="ACHALG_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFN2586109.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            57629..58477
                     /locus_tag="ACHALG_000674"
                     /pseudo
     CDS             57629..58477
                     /locus_tag="ACHALG_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(58830..>59668)
                     /locus_tag="ACHALG_000675"
     CDS             complement(58830..>59668)
                     /locus_tag="ACHALG_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFN2586110.1"
                     /translation="YQLVETQAPTGYELDATPIEFEIERSQTAVVELTKANRLTPGGV
                     VLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGVYQLVETQAP
                     TGYELDATPIEFEIERSQTAVVELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDA
                     NGTVLQSGLTTGADGKLAIDGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIF
                     LTKENRKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKR
                     NI"
BASE COUNT        17829 a        12197 c        10436 g        19206 t
ORIGIN      
        1 ttgtcttgaa ttgaatcaag acgccctaca catttggttt gtcttacttt gttcagtttt
       61 caaaggtcta cttcatcgag agtgtttccc gattcatttt tggctgccgt agcaactttt
      121 atatcatatc agagagacaa acgattgtca actctttttt aaaagttttt ttcgatttcg
      181 tttttcgaaa tctttcagca actcatttat cataacttat cgtttgttat ttgtcaagaa
      241 cttttttgaa gtttttttcg atagaatcaa gcttcgcaag tggttctttt taacgactta
      301 gttatcttat cacgtctaaa aacatgtgtc aactcttttt tggaattttc ataaaatgtt
      361 tcaatgaagc gcgacaacgt tgtttaatat atcattaaaa aaaataaagg tcaagctaaa
      421 atattattta agcttgacct tattataatt tcaacgattg tcaaaagtat tgtttgcagc
      481 tgtgttgcca gtttctatag ataaatcaca gcttttgaaa cataagtttt gtactgcttc
      541 gattcgcccg aattcttttg cagttatttg gcaatgagaa aagcagacat cttctatagg
      601 cttttcagga aatgctacta aatcaaaagc tcgggaagga agtgagtaac ctggagaaag
      661 gcatgcctgt acgtttttga tgtgtatgtc tttgactttc gtcatttgct ttgattcagg
      721 tatttcttca gccactgctt gccaactttc tggcatttga gcaaggtcat tgctattttt
      781 tctattgtat tcattatgcc agtccattat ccaagaaaaa ggaaattgta catcaatcat
      841 attcagattt tctaccctga tattttcgat cactccccct ctttcttttg aagatttcat
      901 tcgaatacca cagtctgact gaaaaaaatc gatatcgtga atatatacgt cactaactcc
      961 agcactaact tcgctaccaa tagttacacc gtatcctgaa cggattttac atctagcaac
     1021 ctcgattctt gatgattttc gattgactct tgcaccattg ccatctctgc cagacttaat
     1081 agcaatacaa tcatcaccac aggccaaatc acattcatag acacgcacat ttgttgaaga
     1141 atcaatatca atcccatctg tacttggtcc atcattgttg cggatgatta ctttttctac
     1201 taatacatcg ttagaataag tgatttgcaa gttccaaaaa cctgatttct gaaaaatcaa
     1261 atcacgaaca acaacatgtt ctgcatgata cagtaaacaa gcgcgtggac gtttaatagc
     1321 ataatccgca atccaacgaa gtcccttttg atcgtattca gctcttgtcc cgcttttttg
     1381 atcttttccc caatacagct cccaccaatg gtctccttga ccgtcgatta ttccttttcc
     1441 ttctataaga atatctttcg ccgagagaac attcaaaatc gctgctggcc atttcatttc
     1501 tacgccggct actcgtgtat cgatttctgg aaatgcttcg atatctgttc tccccttgag
     1561 gactgcacct tcttcaaaga taagatgact cttatctttt aaaaaaaggg ctccgactaa
     1621 ataaatgcct tttggaataa atacaggtaa gtgttctttc gcaccttgat cgattgcttt
     1681 ttgcagtatt attgtatcat cttgttgagt gtcgtttgtt ttaaagttca aagcattgat
     1741 aatccctttt tttgtccgca atacgtttct ccctatcttt cattgatttt tttcaattct
     1801 tcaccaataa atacagtttt tgaacgattc agtgggtaac ctactagcaa aatgattgcg
     1861 gatacaaata agcagaatgc tggtaacaag tttgccatcg tataaatcct ttcttgtact
     1921 aaaactgatt ggatggctcc acctgttgta gacgattggt aaccaatgag tgccagcata
     1981 aatccgccta ccgctcctgc acatgcttgt cctacttttc tcgcaaaaga atttactcca
     2041 tatactgttc catcttctct taagcctgtc ttatattgat gataatcaat cacgtcagta
     2101 atgaatgccc agaccatcaa attgaataaa ccagatccta aagtagcgat aaataaaaga
     2161 atcagatata cccatgcatt tgttatatga aggaaataca tcagcaagta catacatgaa
     2221 gaagcaagta acgcaaatac gcttgcttcc tttttaccaa ataattttgt cagctttgta
     2281 gcaaatggtg ctaaaactac tactgttccg taagtaaata aaagagcaac agacattgct
     2341 tgtttattat ggaagtaatc attgaataaa taagtcatat tcgtcccagc taaaatctga
     2401 ttgatcacaa tgaaaatgtc aacaatgact aaaataatca gtgctcgatt agctaacaaa
     2461 ccttttacca actgagaagc agatactttt tcgtttttga caactcttac tctttcagta
     2521 gtcaatttac aagttaacat atatgcgaca aatgctattg cagcacatcc gattgaaatc
     2581 caaaagaaat gattccctga aacgacttgt tgtccactct tgttatgggt atacattaaa
     2641 atcggaatga agaatcctgt agttgatccg ccaatcgcag aaccaatgct tcggaacgtg
     2701 gacaacgagg tgcggtcatt aggatttgag ctaattgctg ctgccattgc tccatacggg
     2761 atattgattg ttgagagaaa aatcccataa acaatgtatg aaacaaaaat ataagcaatc
     2821 ttcaccgtca ttggaagatg ttggacgaat ggcaaaaaca aaatgacagt cactaagcaa
     2881 aatggatact tcatttttct gatccaagga ataaatcggc cagtttctgt taactctgag
     2941 acatcacata atcttccaac catcacatca gcaaacgcat ccaaaattct cgaaatcaag
     3001 aataacgtac ctacgattgc ccctgaaacg ccaagaacat tcgtataata aatcatcaag
     3061 aaactattga ctaaacctaa aataaaacag ttacctagat caccgaacat gtagcctatc
     3121 ttatcggaaa tcccaaattc ctttttttct ttttttattg tatttgctgt aacagcattt
     3181 tgtgaaagca tttgaattcc tcctacctct tctagtaaac gttttcaaaa ttataactct
     3241 ttttttcttg tttttatatt cacaatgttg gctttttatt gcattatctt gacagataaa
     3301 aaacaaaaaa taatctaaaa acattgatgc ataagattga taactttttt tacataacaa
     3361 tatatacaaa caaaaaaatt cactaactag tgaaaataag ttagtgaacc tcatttatta
     3421 aagaattaaa ttacagatga tcttttccat catacttgaa aaactcaaaa tcagcataac
     3481 ttcctctttt tttatttaga tcatgcactg ctatcccgat aaaatttcca gtaaaccctc
     3541 ctgagagtat acacatgttt ttttgcggac ctactttctt ccattcttca gttgatacgt
     3601 ctttcaaatg aaactgtgct gtctctttct ttacagtgac tttcaaaaaa gtcttctttt
     3661 ctgtgagtgg aatcgtccaa tcttctattt gtgctactcc gttttctgat tctatcatac
     3721 gtaggcattt tcccttgtcc tcattccaag taacataaca ataaatataa ttggattcat
     3781 ttaagtacag taccagtcca gccatctgat taaaattatt aggcatgtat tcaaatgctg
     3841 tctccgcatc aaaagtaaaa tccgtttgcc gaatagctaa caaatgatgt tcgaaggtag
     3901 attgaagaga ttcaccggaa atcagacgta aaaaacctgg tctacatttt gtatcacacc
     3961 aatcagcatt tggcaaaatg cgtctggcat tccagttctt ctttagctct ccttcaaatg
     4021 tatccataaa ctgatgatct ttttcttgtt gtacctcttc atttgtctca atcattattt
     4081 cttccatcgg agttgtctgg ctatctttaa gtcttaacca atcgtcggac gtccaataca
     4141 cttcttgaat agcggtttct cttcccaaaa tagccgcacc gtttaatggt ctggtacaaa
     4201 gatatgtcat gtaccatctt cctgacgggg tttgtactaa gctcccatgt cctgtacatt
     4261 gcaatgtaga agcaggtttg tcactagctg tcagcatagg aaaaccagga tcaagctcgt
     4321 aaggaccagt aatattcttt gctcggcaaa ctgtaactga atgtcctctt cctgtaccgc
     4381 cttctgccgt catcaaataa taatacccgt tacgaaaata caaatgtggt gcctccgttt
     4441 tcgctaattc tgtacctgca aatattttat agatgggacc atcaagcttc ttcttctctg
     4501 catcatattc ttgtaatacg ataccgctgg atttattcgg tgtgtccaga cgataatccc
     4561 atagttcatt gacaagccat ttttttcccg tttcaagatc atggaacaaa gaaggatcaa
     4621 agccgttact atttaaataa attggttcgg accaaggacc tgtgatctca gatgcagtga
     4681 tcaaataatt gtggacatct ttaaacggac ggcctgcact tttgacatct gtgtaaacaa
     4741 gataaaacaa tccatcagca taactgatct gtggcgccca tatacttcca tttgtaggat
     4801 ttccttgcaa agatacttga gtggtcaaaa tatccgttgt atgtgaccag ttgactaaat
     4861 ttttcgactc atatactcgg attcctggaa gccattcaaa agaggagaca ataatgtaat
     4921 accgctcttt cactcttaat atattaggat ctggattgaa tcctcttaaa ataggatttt
     4981 tcactttgat agtctgcatg attcaggctc cttttctcat tcttactcga tataaactga
     5041 atgccccagc tgcgaaataa aaaaatgcaa aaggaaacgc aataaactga gtgccaatca
     5101 acaatatgaa tcctgttata aaatacaaac cagtggcagt tgttaagtat tttcctttag
     5161 cagtcaaaaa caatgctact gctaaaaaag caagtgaaat aaacaacaca acggcaaaca
     5221 gaccagtcat gtcctgtaat aatccaaatg tattttctaa agacaaagtt gaatgatcta
     5281 cagcaagcga ctgataactt tttttaaact ctgacgcaga catattactt aaagtaactg
     5341 ctaacccgcc aaagaaaatc aaagatagac tgccaccgac cattcctagc aatagttccc
     5401 attttctttt catttctgaa ccccctgctt ttctaaatgt atccattcag gtcttttgat
     5461 cgatgtttca taattgttat tacgttcaga gaatttgaca gtctgttcat ttttcttgtc
     5521 atcccaatta tcccaaccag ttgggtgaac atgcccacct atcttacacg cttcaaataa
     5581 gacatttgca tacggacgcc acggtcttcc taaatagacg tttctagtat caggttctgc
     5641 agtcaaggta cagctttcaa aacgataccc tgttttttga tttttcggcg tagatgctgc
     5701 tgtgatgtat ccaggatttt tactggctaa gctatggata tgacactgat gaaaaacggc
     5761 atctgcccca ccaaatatga aatccaccgt tccttcaatc cagcaatatt cataggtctg
     5821 acgacaatac tcaggattgg cagaagccgg tgtacaaaaa ggagtaccat ctttttgcaa
     5881 atcaggcaac ggacccgtgc ataaggtatc ctgataacca gatagtcgac agttttttaa
     5941 agatacgcga tctcctgaat tgaataaagc aacagcctgt cctacctttt ctcctggacc
     6001 tgctgtattt tcgatgtgaa gattttctaa tacgacattc gatccttcta agaaaaaagt
     6061 agcacttcgg aaagtcccta actggctacc gtcaagatac tcctgttttg caaaacgatt
     6121 accggagata actactgttc ctgctcccac aagtttaaaa ttggataatc tgctttctat
     6181 atattcatca tatcttcctt cttttactat caagaccttt ggatggctat ccggcaggcc
     6241 tgccaaataa tcgattcctt cttggatagt ttcaaaatcc gtcggggtat ctatagcatt
     6301 ctttccaatt atcagtttgt ctttcatcct cataaatgct cccttatgaa ttgtttccaa
     6361 atatttctca tttcttgtgt ttccatatga ccgcctgtta aagaaaaaga agaaaagttt
     6421 tctggactac ccattgattg atactttttg tccagccctt ttctcagctt cagtacgcct
     6481 tcgtccaagc acatcgtatc gttttttcca actaagctaa gacgagggcg cggagcgatt
     6541 ttttcttgga ttgctaacgt tgtataagct gtcaatagat ttggcacata gtaataaaac
     6601 ccatgatgat ctaggcggcg atgtagcaaa agtatttcga tgtccacctg tgccgctaga
     6661 tcaatacaaa ctttgacacg agaatccaat gctgtcaccc accagcttaa taagcctccc
     6721 atagaaagac caatcgttgc gagccgttga gtatctgtgt cttcccttgt ctccaaataa
     6781 tcgatcaagc tgatcacatc ataaatacgc attccccata acgtttttcc tgttaataaa
     6841 aattgtttga acaactcgct ttcagaaata cctccgcgtt cttcaaatcc ccaagcatca
     6901 atcgaccaaa cagcatatcc cattcctgtc agtacttctg caaaagatgg agaagctaag
     6961 taaggagcac catggatcaa ttcacttttc ccgactgaaa aatctccgcc gtgagaatgc
     7021 aggtaaagga ccggcggaaa cggtccttct ttttctggat aagcatagag ggctggtact
     7081 gtttccaatt catttaagta caattgatag tattcaagca taaaaccgtt ttgtttttct
     7141 cggtataatc gttttccact aggggtatgt ctgctagatg gagcacctaa aaaatcattc
     7201 aactgcataa gcaaagtttt tacgaacaac cagttcatcc atattttctg attgatttga
     7261 ttggaatttc tcaatactga tttctttagt attttctaat agaaatgctg gtccctcgtg
     7321 attttcaatc gacatctggt taaaggaaac cttacccgca aagccaatat aaaatccttg
     7381 tctgctcatt tcctctattc ctgtcatcat agcaggaaca ccgggaactg catcttgggc
     7441 catcgagaca tcgatattgt caaatgtgat ttcttgtatg aattgttccg ctaatccata
     7501 aataaaacct gcagaagcat ggacattcct tgccgtgata ttcgaaaaat ggatccttct
     7561 gaatgctggt gtccgttcat ctatcggata agcctttttt tcccatacat acggttcttt
     7621 ccctcgcgga ccacagaaat aataaagatt cagtataaat ggacacatca cattatccat
     7681 gactatattg gatactcgaa tatcttcaac gatcccgcct cgacctcgtc tagatttcaa
     7741 tcgtattcct cgatcagttt cttggaagat acaatttgaa atggtaatat tacgaatact
     7801 accactcatt tcacttccta atacgacacc gccatgtcca tggaccatcg tgcaatttgt
     7861 gatcgtgata ttttcgcaag cgatcctttc gtacgtatct tcagtccctg ccttgattgc
     7921 aatacagtca tctcccacgt caatatggca attactaata cgcacatttt tgcatgattc
     7981 tggatcaatt ccgtctgtat ttggtgaatc cgctgggttc aagatcgtca agttatcaaa
     8041 cgttgcatta ctgcaaagaa tcgggttgat cgtccaactt ggtgactgga tcaatttaat
     8101 atctttcaca gtaattcgat gacagttatg gaaactcatc aatttaggac gaggataagc
     8161 gagattatct ggttcatttc taaaagtatg ccaccatttt tttccatttc catctaatgt
     8221 accgaaacca gtgacagaga tgttctctac attttgagca tagatacagg aagcataaac
     8281 ttttcgatgg actccttccc aacgtgaatg aacaacagga taatcttttg gatcgtcaga
     8341 gaatttcaga accgctcctg cgcttaaatg aagttcgaca ttgcttttta aaaataaagc
     8401 tcctgttaga aattcacctg ctggaacaac tacggtcccg cctccgtcac ttgctgcagc
     8461 atcaatggct tgttgtattg cttcagtatt caattcatca attgatgcgc caaatgtcaa
     8521 aatatcatac atatttataa tgcctccact tcaatcaatg cttgcaagaa tgcaccaacg
     8581 cctttttgat cattacaaat aattggttca ctgatgtagt aagcatatga accatctcgc
     8641 tgatctgctc cgcctaaccc tgctacttga cagtttttat tcaagttcag ccagccttct
     8701 tttgtttcca gaacaaattc atcaagtaat ccttgatgtg atttcttcac ttgtggaagc
     8761 cattcttctt tgttcagcag tcccaaacgt attcctttag ccattgcgta aacaaacata
     8821 ctactgccgg aagcttccag ataatttcct ttccgttttg tttctgttgt tacttgatac
     8881 catgtcccgc tgttttcttc ttgatatgga agtaatgcag ccagtgtctt ttctagaatt
     8941 tctttcagca aagaacaatc tgtttcttca tgaagtaatt cataggtatc tgctgcagcc
     9001 atcaaatacc agccaatcga tcggctccaa aaatgatggg atagacctgt ttttggatca
     9061 gcccatggtt ggaccttctt ttcatcccat gcatgaatca gcaaatttgt ttttggatcg
     9121 aatagatgtt tataactgat ttcaaactgt ctcaaaacat cttggtaatc tttttcttca
     9181 ctaaaattct ggataaattc agcataaaaa ggagaaccca tatataagcc atccaaccaa
     9241 atttgataag gataaatttc tttatgccaa aaagctcctt cggatgtgcg tggatggttt
     9301 gccaactgct gggaaaggag ttctgctgct tttttatatt tttcttcgcc tgtttctttg
     9361 tacaataaaa agaaaagctt tcctgtattt acatggtcga tattgtattc ttcaacgctg
     9421 tagccacgga tcgttccatc ttcttgcacg aaatagtcga tattgtcttt tatgaaatca
     9481 aaataaattt tttcttgggt tcgttcccaa agtaattgga accctttaag tacgacgcca
     9541 tagtcataag accattttcc tttataccac ttttcttcat ataagcgagg tgttctttcc
     9601 atgatacttt ttgctaattt ctttgaccag ttttccatta tttgctctcc tttaaaaatt
     9661 aaaataggat ttgggacaaa taccaagaag tatttgtccc aatcgtctat attactgtgc
     9721 cgctgctttg tatgcttcct cgtactcttg tgtgattcga tcgcctcctt gtgctttcca
     9781 gtcttctaca gcttttttga atccatcttc gtcaagctgt cccatgatgt attgcaccgt
     9841 agcatcaatg acgatttttt caatttctga tcctacttca ttgttcgtcg gtgaatccaa
     9901 tggcacagaa ggatccatta cagcaaattc ttcattttct ttgatcaatt gattagagaa
     9961 ttctttttca gggtctgtat ctttgaaaga gaacgtcact tcactaggac gagagcttga
    10021 taacggttgc acctcttgtt gccaaagatc catatcgata aattcgatag aaccatcatc
    10081 attcaattca taatgcgttc cttcgatacc gccagtcatc aatttataca catcttcttc
    10141 taaaagatca tcaacaaatt gaagtaattt cttcaattgt gcttcgtctt ttacttgaga
    10201 tttgggaaat gctagcaagc cgccaatacc atttccttct gaccaaacat gataaccgcc
    10261 gccttcacca tctgagatct tgtttacagg cactaattcc atatcatctt gaataccttg
    10321 acccatcgtc ttcagattct tgatatccac cattccagtg tagattccag ttcttccttg
    10381 tgcaaattgt tcttgctgat cagtcttttg ggtaactgca aagtcttgtg ccaaatagcc
    10441 attctcaaat agatctcttg aaaaattcaa agctttcata tactcttctg tatcaaattc
    10501 tggagtgaat ttgccattat cgtctacagc ccagccattt ggtgctccaa aataagaagt
    10561 gaaaagttta aagctggtat aacgaatatc actaggcgca cgatcgccaa aaccaactgt
    10621 gtcgtccaca ccatttccat caggatcgtt ttctgtaaat tgtttagcta cttcataaag
    10681 ctcatccatg gtagttggaa catctagtcc tagattatcc aaccagtctt ttcgaatgac
    10741 taagccagca cgagcgtagt ctttttgtat cggtacgccg tacaaggcac cgtcgatact
    10801 cgcagcttcg attctatctt cagaaatttg acttaaattt ttatagtctt ttaaatattc
    10861 tgatacatcc cagaatacgc ctgattttaa cgcattgcgg acagaagagt ttttcaacat
    10921 cgcaatttga agtgtaacga tatctcctag ttcatcagaa gccaatgcag ttgttaatct
    10981 ttcttctttt gaagcatcag gaatccagtt gaatgtcagt tccgtatcgg ttttttcttc
    11041 gatcaagttc acaatcgtgt ctgtaggcgg tgaagcatga tgcaaaatat ttaaccaagt
    11101 gattttagct tttccatctt ctgtctctga agaactgtta cttgaactgc tgccgcatgc
    11161 ggcaagtgct agaattcctg caaaactaag acctaatttt acgagcgttg cttttttcat
    11221 aaattttctc cttcactgtt ttaataatat ttttgttttc ttctgttttt tctgtatatg
    11281 agttcttcca ttccaaatgg caaaatatag aatttgctgt ccacgtgact gcggaaaaga
    11341 agagactgcc accgataaag gttaaaacac ctggcattgt ttggatgatc atcagataaa
    11401 tccctaccat acttaatatc aacactaatg agtaatgaag atgtgagaat ccgaataaaa
    11461 atgccatttt cattttgaaa aaatatcctt tccaatcata atgagccatg attgggaaaa
    11521 tatagagtgc tgagagcaca tatatgaacg cagcaatata caaagcgatc ctaagatacc
    11581 aattgtgtat gaaagaaaga tccacaagta aaacaatccc gatggcgcca tatatccagc
    11641 tcatcagcaa ggtttcacga tagttttctt taaaatatgt gaagtacgtt ttcgtcaaag
    11701 gaaacgtttc ttttgagcgc agccattttc taatcactgc aaccatcgca tacgatgcag
    11761 gaccgaaagt aaaaactccc aaacctgcga tgactaaacc gccccacaga aaattcaaat
    11821 aaaccaactg taaaatacga tttagccttc cgtttatttt atctaatgac tgcagcatat
    11881 ctttccctct ctttctatct tagtaaacgc cgtcttctcc tagctttttg gcaatcttat
    11941 tagctaaaat taccataaac aatcctacga cccctttaaa caatcccatt gctgtactga
    12001 aacttaactg tccattcttc aatccagctg tataaatata ggtgtcgaaa atttctgcaa
    12061 cgcttcgatt caatgaattc aataacaagt acatatgctc gaaccctaag tctaatgtct
    12121 gaccgatttt tagaatcagc aaggtaatga ttactggacg aattgccggc aatgtaacat
    12181 gccatgtttt gcgtaagcgt cctgctccat ccatttctgc agcttcatac aactgtgtat
    12241 cgactgcagt tatcgctgaa aggtagatga tcgtagacca gcccaactct ttccagatca
    12301 cttgtccgat atatactgtg cgtgtccatt caggggatgt tagaaaactg attttttcaa
    12361 atcccattgt tgcaaggaat tcattcaata cccctccgtc aacattcaaa aatacataag
    12421 taatcgaaac aatgattacc caagacatga agtgagggat gtaaatgatc gtttgaatcc
    12481 cgtttttcaa tcgtttgttc gttacttcat ttaatagtaa agacaaaatg attggcatcg
    12541 gaaaaaacac gaagatattt aatccgaaca agatcaacgt atttcttaat aatgtaaaga
    12601 atgtcggttc tgtaaacaaa cgaatgaaat gtttgaaacc gacccaagga cttcccatta
    12661 ttcctaaaaa tggttgataa tcttgaaaag caataacgag tccccccata ggaagatacc
    12721 taaaaatcaa gaaataaagg actcccggca gaatcatcac atataaaaat ttattcgttt
    12781 tgattcctgc aattattttt tttcgtcttc tgagcttttc ttcttttttc gatagcgtca
    12841 gttgaaatgg ttcttgtttc tttacacttt ccataagccc ctccttagtt ttgtgaatgt
    12901 ttgctgctta tgacttcatg atagataact atctgaatta cgtatataat catttttacc
    12961 gtaagtaaaa acgcttacat agcaatgatt tttgtgctat acaaaacgat aaagtgtatt
    13021 ttttcacaaa taaactacgc atagcaaacc ttctattttt ttctatctga cacttagcat
    13081 aggaaatgat tatatacgta atctttcacc gatgataaat tgaacatata gaaacagaaa
    13141 catttaattt attttgagga ggagcctatc tatatgaaaa agaaagtctc tataggagaa
    13201 agaagtttta cacttttcaa caatacgttt ctaattttgc tagctttgat ctgtatcgtt
    13261 ccttttttaa atatcatcgc tacatctttt gcttctacac aagaagtcgt cgcgaaaaag
    13321 tttatccttt ttcctactac cttttcttta gacgcgtatc gctacattct ttctacgccg
    13381 accattttta gagcacttgc tgtttcgatt ggtgttacag gtgtcggaac gatcgtcagt
    13441 atgtgtgcaa cttcactgat ggcctacggt ctttccagaa agtatttgtt tggtcgaggt
    13501 ttcgtcaact ttcttgttgt attttctatg ttgttcagcg gggggatgat tccaacgttc
    13561 ttagttgttc gttctctagg gctggtcaat tcttactggg ccatgatcct gcctgtggct
    13621 gttaatgcaa tgaatatgat cattatgaga aactttttcc aagccttgcc ggatagtttg
    13681 gaagaatcag caaaaatgga tggttgcaca gattttggtg tatttttcaa aattatgctg
    13741 ccattagcgt taccgtctat tgctactatc tcgctttttt atgctgttac ttactggaac
    13801 acttatatga ctgctatttt gtatatcaac gattcatcta aatggccgat acagattctt
    13861 ttacgccaaa tcgtcattgt atcaagtggg atgcaagcag aaagtagtgc cgttgatgtg
    13921 attccaccag cacaaacagt taaaatggct gttatcgtta ttgcaacagt gcctatgctg
    13981 attgtttatc cttttgttca gaaatatttt gtcaaaggag cactcgtagg ttctgtcaaa
    14041 gggtgattat gtactgaacg caaaatggta gttgtgtcag aagtggtcag cttcaagaaa
    14101 taagaaggca tttacgaaaa ttgcttctta aatttttgtg aaatttcggc ttatttttcg
    14161 aagaatctac tcctggaaca ccgtgtatca gaggttgtga aagagacgtt cagccttgag
    14221 cgatgataaa aactctcaca aacattgcga cgagtaaccc acaggagcaa taagaagcga
    14281 ttttgaaaaa tagcttccca tatttcacaa aattatgcct tattgtcgat agcctgtctc
    14341 ttgaacgcca tttattcgta aaaaagaact gtgacaagac ttttgtcaca gttctttttg
    14401 cgtgcttagc cttgaaattt aactatccat aaatacaaat actagggtta ttctaacttc
    14461 ttttattgct tgaatcagtc cacaagaaaa acagcctctt gattcacaaa tatttttttc
    14521 gatattctcc cggcgtaatc gattccttct ttttaaagaa acgaataaaa ttttgcggat
    14581 ttcgatattg caatctttct gcaatctctt ttacggagag gttagtctct ttcaaccatt
    14641 tttttgctac ctctaaacga tgattttgaa caaaatcacc aaatttttct cctgtttcct
    14701 ttttaaatac attacttaaa tagttagggt tataatgcaa cctatcagcg atcacatcta
    14761 aagacagatc acgatcatat tctgtgtaga cgatatgcat aatattctcc gacaaatttt
    14821 taaattcatg atcagtgatt gcttgagagc attctgtgat tggcagaact aattgttcta
    14881 aaacaagttt ttctagttct ttgggatgat aattgttcag tgcttttaca taaatttgtt
    14941 tcatgttatc aaagtttttt gaatctactc ccaataactg tcctaattga acgagttcat
    15001 tgatcaagcg gaccgaaact acttcccgac tcaaaggatt tttattttgt gcaaacaatg
    15061 catcaatcaa ttcatgtact aaattcttca cttcctgatt tccagaacga atggcttcaa
    15121 acaatcgatt ttgttcttct atcggatatc taataagtgt tttttcatga gcagcaggaa
    15181 cgatttcctg ataaaaaata attgaacttg gaccagtatt tacacgataa tacagggctt
    15241 cttttgcccg atcaaccgat tgcttgcttt cattcagcgt ttcgaaccgg ttactgatgc
    15301 ctatactgat agttaaattc aagtattctt ttacacgttt ttgaatgagt cggcaatact
    15361 ccatgacttt tttgcttgct tctgcatcat ctttatccat tcgataaata gtggcttgca
    15421 tttcttcatt caaaacgatc ggcagcattc gattttcctt tggcacaatt tccgtgatca
    15481 tgttgttgat cgctaaaagt agcaaatcac gctccccacc gtgactatct tctaacagat
    15541 cgatttgaac caaagctgta taatagcagt cattttgaga actataacca aattgctgca
    15601 accgctgatc tagttctcgc ctttctactc gatttctaaa taaactaagt acaaatagcg
    15661 tttccagctg tggcttttgg gtaactaagt gtgcacttaa cgcttctttt tctcctatga
    15721 tccgactgac tgattgagcg accaaatcta gttctttccc actgaatcct tttgatttaa
    15781 gattcagacg ttcctgtatt tgagaaatcg gacgggcaaa tctgtccgaa aataagtagg
    15841 aaagaagacc aatgagtcct accagtgtta aagacactgc aactagcccg attctcagat
    15901 tgcggatcat tgcgccaatt gcactagagt caacttctaa tctgtaaatc caattattat
    15961 aatctgattt cagtactata tacgttttat ctttttctct tatcactttc agctgattgt
    16021 aatctagatc cacgtccgtt aatacagttg gttgctcttt attttttact gtctgagtag
    16081 taaaaaggtt gccaacttcg ttttcaatag aaagtatgtc ttctccactt tcaccaacaa
    16141 cttgttctat tgtatgtttc tctatcgtag caattcccaa tgcatatttt tctttttcaa
    16201 acattggcaa agaaatcacc atttcgattc ctttgttttt cttttgccaa aataaattat
    16261 ttttgttatt tatgtacttc tgttgatact tttcaatttc ctcttcggtc agttgactca
    16321 acgatccatt gattactccc catttaccag ccaaactgac taaatcataa ttattatcat
    16381 ccataacaat cgtttctata aaatttagtt ctttcctgat ttcagaataa gtggagtaat
    16441 ctgtatatgt cagcggatgc ttgaatatgt ccgagaaatt tttggtcgaa ccatacgtag
    16501 aaaacgaata ttcaatcgtt ctaattttcc attcaacgtt agttttaact tgcctgagca
    16561 aattcttttt gtctgttaaa taactcgatt cgatcgattc ataggtattc tggtaaatga
    16621 ataaactaaa tcctatgaca agtacggctc ctaataaaaa catcggtaaa aatattttat
    16681 aaaatactgt acttttcttc attttcttcc tctcctcctt ttaaaataaa aggctttcat
    16741 ttttctgtaa aattgatact catctaatta aatgagcatt ccccgctttc aagaatagca
    16801 taattaaaaa taaaaatcca agtaattttt aacgtgtgca atcttttcat atcaaagatc
    16861 tattgactta tcgtagttat cgtaatatga tgaatgcgtt aacaaaaagt gaaaaagtaa
    16921 ggaaaatgat ggaatggtaa cgatcaaaga tgtcgctaaa gcagctggcg tttctcatac
    16981 gacagtttct cgtgctttga ataacagcag tttaataaaa aatgaaacaa aacaaaaaat
    17041 acgacaacta gctaaggaaa tgaattattc gccaaactat agtgcaaaag gtttagtgaa
    17101 tcaaaaaaaa tatctaatcg gactattttt ctcaagtttg cataaaggaa cttcatcaaa
    17161 tttcctagca gattccattg caggcatcca ttcagtatta gacacaaatt acagtttatc
    17221 tgttgaaagt atcgatgttg ttgatttaac agaaatcaac ctgcaacgct acgatggact
    17281 gatcgttatg agtcaaagtg atgatgatca gcctttcatc gactatctca aagaaaaaga
    17341 atttcctttc gttgttgtca atcgtcacat caatgatcca gcggttatca acgtcactgc
    17401 cgatgatgcg gccggtgtta tgcaggcagt agattacggt attggatgcg gacatacgaa
    17461 gatcgcttat attggcggaa agaaaaattt tcgttcgaca aaagaacggg aaaaaggggt
    17521 attgaaaagt ctaaaaaaag cgagcatccc tgtcaatcca gcctattttt taacaggaga
    17581 ttacagctta gaaagcggcc tccaaaacat ggaataccta ttatcacttt ctgacctgcc
    17641 tactctggtt ttttgcggca acgacgacat agcgattggt gcattgagag ctgtacattt
    17701 agctggcctc cgtgttcccc agaacatttc tctgatcggg ttcgatgatt ctccagtcgt
    17761 ctcctatctc aatccgccgc ttaccaccgt acgtaagcct ttgaaagaaa tgtgtcaaga
    17821 aggaacaaag cttttactag atttgatcga aggcaggacg atcgaacaat ctaaaattga
    17881 gctctccacc ttgttgatcc ttcgtgagtc gatcaattac ttgtaaacta caagtaattt
    17941 tatataaaaa tgttaacgtg tgcataacac agaaaggaag tttatatgaa aaaactgaca
    18001 aaagaattag tgaaaaaaaa cgaagcacct gtaaaagttc ttcagtttgg tgaaggcaat
    18061 tttatgcgtg gatttattga ttggcagatt cagcagctga ataaccaaca attgtttaaa
    18121 ggtaatgttg caatcattca acctttagaa caaggtcttg gaaatatgat gaaccagcaa
    18181 gataatttat atacggtcat tttacaagga ctcagcaatg gcgaaatcgt tgatacttcc
    18241 gagattatca ccgtagtaga acaaatgatc aatccttatg atgaatggga tgcttatcta
    18301 tctctagcag aaaatgacga cttggcctat atcgtctcca atacaacaga ggcaggaatc
    18361 caattcgtgg agactgataa tttaacagat actcccccac agagcctccc gggcaaactg
    18421 acagcctttt tataccgacg tttcaaatta aacaaagctg gctttaccat cattccttgc
    18481 gaactgattg acagaaacgg cgaacgtcta aaagaaatca ttttacgcta cgcagaaaaa
    18541 tgggatttag aacctgcttt tgttgagtgg ttagaaaaag aaaatgtttt ctgttgtagt
    18601 ttagttgatc gcatcgtccc aggttatcct cgcgaagatg caggtgtttt caatcaacgg
    18661 cataactatg aagatcaatt gatggtcaaa gcagaaccat tcatgctttg ggtgatcgaa
    18721 gggcctaaag aattggagaa tcaactacca ttcaaacagg ctggcttgaa tgtcatcatc
    18781 acagatgata tgactcctta tcgtgaacgt aaggtgcatt tgcttaatgg accgcataca
    18841 gcgatggtcc ctcttgcttt gttggcggaa ttggaaactg tagaagaagt aatgaaagat
    18901 cctttattct ccgcatatgt tgatcagtta ttcaatctag aattgattcc tatgctttct
    18961 ttacctaaag atgaattagc catttatgct gaccaaatca aagaacgttt cttgaatcct
    19021 tttgcccatc acaaattaga agcgatttca ttaaatagtg tttcaaagtt cagcacccgt
    19081 ctgcttccag tgttcaagaa gtatatcgaa gaacaaaatc aggtaccacc gttaatcact
    19141 gtttcactag ctgcgctact tcttatgtat cgaggcgatc aagtaaagcc acatgatgat
    19201 gaaaagacga taagcgaatt tactgatgct tggtctgata atggaacagc tattcctcga
    19261 ttattgcaaa acgctgcgct ttggggagaa gatttgtctc aaataccgaa tgtgacagat
    19321 acagttcaag aaatagctgc aaagatcgat caaaaaggtg tccggtcagt attacaagaa
    19381 atgaaaggaa cgatggtcca tgcttgaaac cctgcaacca acaatgaaat taaatcgacg
    19441 agacagtgtg gtcgtcgcat taacccctat tccaaaacaa acacaactga cgattgatca
    19501 gcaaacgatc accactcttg aagatatccc gcaaggtcat aagattgcat tgggtgattt
    19561 gaaagctgga gacaacgtga tcaaatatgg ctatccaatc ggtcatgtga cgacagacgt
    19621 aactgccggt caatggctgc acactcataa tgtaaaaacc aatctttctg gtgaattgga
    19681 ttatcgatat gaaaaagatg tccatccaag tcattacctt tttgagaacc gaacatttca
    19741 aggttacttg cgaaaaaatg gaaaagtagg gattcgtaat gatttgtttt tagtacctac
    19801 cgttgggtgt gtgaatggca ttgctgaact gatcgtcaaa cagttcaagg aaaaacatcc
    19861 agatctggga cagtttgacc atattacgat tttaaagcat ccttatggct gttcgcagct
    19921 tggaaaagat catcaaaata ctcgagaaat tttagcggac gcagtaaatc atcccaatgc
    19981 cggaggagtt ttagtatttg gattaggttg tgagaacaat acagttcctg aattcaaaaa
    20041 aattttaggt gaatatgatg aagaacgagt gaaatttcta gtggctcaag atgtttacga
    20101 tgagatcgaa caaggtgtgg ctctattaga agagctttta acagcggcgg aaaatgacca
    20161 gcgcatatca gtcccgctct ctaagttgaa tgtcggtttg aaatgcggcg gttctgatgg
    20221 tctatcggga ataactgcta atcctttact tggtgcattt tctgactact tgattgctca
    20281 aggaggcagc acgatcttaa cagaagtgcc agaaatgttt ggcgcagaac aagtattgat
    20341 ggcacgagcg gaaaatgaag aagtttttga ctcaatcgtt gacttgatca atgacttcaa
    20401 acagtatttc ttgtcttacg gggaaccagt ttacgaaaac ccatctcctg gtaataaagc
    20461 aggtgggatc actacattag aagataagtc tttaggctgc acgcaaaaat caggaagctc
    20521 tcctgtcgtt gatgtgctac aatatggcga aaaaatcaga aaaccagggc ttagtttatt
    20581 gcaagctccc gggaatgatc ttgtagctgc ctctgctctt gcttcatccg attgccagtt
    20641 ggtcttgttt accacaggaa gagggactcc gtttggctca tatgtcccaa cggtcaaggt
    20701 atctacgaat acaactcttt ttgaacgcaa aggtcattgg atggatttta atgcaggcgt
    20761 cttattagaa caaccaatgg aagtaatctt agaatcgttt gttcaaaaaa tcatcgctgt
    20821 agctagtgga gaagaaacga aaaatgaaca aaatgacgtt cgagaaattg ccatttttaa
    20881 aaacggagta acactttgat tttcataaaa cagtataaaa agaaagaagg aagatttatg
    20941 tttttatctg atgatttttt attaaaagac gattgggcga aaaagcttta ccattcctac
    21001 gcaaaaaaca tgcccattat tgattatcac tgtcacttag atccaaaaga aatttacgaa
    21061 aacaacaatt tcagcaatct gacagaagct tggttaggag gcgatcatta caaatggcgc
    21121 ttgatgcgag cttgcggcgt gcctgaagaa aaaatcacag gaaatgcttc tgattttgaa
    21181 aaatttttag cttggtgtca aaccgtacca aaaattatcg gaaatccgct ttacagttgg
    21241 tcacatcttg aattgaagcg tttcttcggt attgacttgt tgataaatga agaaaatgca
    21301 gagaaaattt ggacacaagc aaatgaaatt ttggcagctc ctgattttcg ccgtcgggaa
    21361 atcgcaaaaa aatcgaatgt agaagtgatt tgtacaacag atgatcccgc agacgacttg
    21421 cactatcatc agttgcttgc tcatgaagaa ccagatttga aggttctacc ttcattccgt
    21481 ccagataaag cactcaatat tgaaaaagaa ggatttaatg catgggtcac actactggaa
    21541 gaagcagctg accaaacgat caccacctat caagaactga tagaagtctt agggcagcgg
    21601 atagaatact tccatcaaca tggatgtcgt gtttccgacc acgctttaga catcttacgc
    21661 tataaagaag cggatgaaac agtattagaa gagatttttc aaaaagcacg agctaacaaa
    21721 ctattatctt cagatgaaat cgatgcttat cgtacagaaa cactgataca tttgacccac
    21781 ttctatcatt ctcataattg gacgatgcag ctgcacatcc atgcttatcg taactgtaat
    21841 acacaaatgt tggaaaaatt agggcccgat accggctatg atggaatgaa tgatctttct
    21901 ttgactattc ctctgcaaaa attattgaac cgtgcagaag aaacggatca gttgccaaag
    21961 acgatccttt attcattgaa tccgaatgac tatcctgcga ttttagcttt gatgggctgt
    22021 ttccaaaaag aacagaatgg aaaactgcag ttaggttctg gatggtggta caacgatact
    22081 cgagctggta tgcgcgatca attgacacaa tttgctgatg gtagtgcttt ggggaatttt
    22141 gtcggcatgc tgacagattc aagaagtttc ctgtcctaca ctagacatga atatttccgt
    22201 cgtgttcttt gtgaattttt aggcgaaatg gtcatgcgag gagaagcacc tgaagatgaa
    22261 caacttttag gaagtctagt tcaagcgatt agttatacaa atgctaaaaa ttactttggc
    22321 ttttttgcct aattgattaa tagtaacagt aacgaatcaa aaacaggata gtgaaataac
    22381 ggaatccgtt tatccacgtt ttagagaaga agacggacca aatttgctcc gtcttcttct
    22441 cttgctgttc tgcttcattg cctataaacc tttatgaaag aagagataac atgcctacta
    22501 tccatattgc cggtgattct actgcggcag aaaaagatga aaacaagcgt cccgagacag
    22561 gttggggaga aaaaatctct gcttattttt cacccgagat ccagatcaat aaccaagcaa
    22621 agaatggacg cagcaccaaa tcatttatcc aggaaggaag gttagcccgt ctagcttcac
    22681 agtttcagcc gaatgatttc ttatttgtac aatttggaca taacgaccag aaaatccaag
    22741 aagaaagagg tacgcttccc ttcggtagct atttaaaaaa tctgcagata ttttttgaat
    22801 gcgcaaaaaa agcgaacgtc caaatggtct tactgacatc tgtgacgcga cgagattact
    22861 tagaaaacgg aacattgaat ccagatatac ttggagacta tccaaaagcg atgcgtgcat
    22921 ttgctgagaa acaccatatt cctcttttag atgtgttctc aagatcacaa gaattgttcc
    22981 agaccttctc aaaagaagag acaaaaaaat tctacctgca tcttatacct gatacttgta
    23041 aaaattaccc agaaggacta aaagacaata cccactttag tccagaaggc gctgataaag
    23101 ttgcccgaat aattgttgaa ttgataaaag aatcccgtct gcctcttgct aattatttac
    23161 aaaaaggagt ttgagatgtt tccattaaat ttaggcatcc gtgcccacga tttagatgtt
    23221 cgaaatcgtg aagaaatcgt tcaaaaagta tcacagcttc acttgtccca tatccaattt
    23281 gcaccacaaa aagcatttcc tgaacaactt aaattaactg acatgacact tgggagtgct
    23341 gcctattttg gtgaatactt ccaaaaaaac aatatcgaac tttccatttt aggatgctat
    23401 atcaacctat ccagcaaaaa tcaagaagtt cgtgcacaag cattggcaac tttcaaacag
    23461 caaattgctc tgtgctctgc ctatcaagcc aagatgattg ctacggaaac cggtagtgtc
    23521 tctgtcggtt acacaaaaga aaattttact gaagaagctt atcagatcgc cagaaattct
    23581 atcattgaat tagtcgctca tgcagaaaat tttggcgtaa ctgttgctgt tgaagcaggg
    23641 atcaatcatc caatctataa ctatcagtta gctaaacgtt taatcgatga agtagcttca
    23701 ccaaatttaa aaatcatttt agattgtgcg aatttgatgc ataaagaaaa tcatgctgat
    23761 caggaaaaag tgatccgagg tgccttggac aatttacatg gttatattac agcgcttcat
    23821 ctcaaagact atatcatgga aaatggaaag atccgcatgg tacctgttgg caagggctgt
    23881 ttagatttcc gcccggtact gcattatatc aaatatgagc gtcctctaat gtacgccact
    23941 cttgaagcta ctcctgagat ttatgttcct gaagcaatag aacacttgca gtttatttac
    24001 caagattgtt aaggagaaga ttatggagga acgaatacag aaaagttatc aaaaatatca
    24061 tgcaaatgct tttcagacat tgctgcatct ttatcaggga aattattggc gttttgccat
    24121 gtcaggactc ttttttatta tcaaacatct tccatcttgg ttaatgccga tcgctattgc
    24181 caatgtaatc aatgcagctt catcgaaaga ttcttctcag ttgcaggcca tttatttcaa
    24241 cgcttttttg atggcttgtc tgattggtca aaatatattg acgaattatt ttcatgtaaa
    24301 ataccatagc acttcgatcc gtcaagtaga agctggacta agagcgaccc ttatcaagaa
    24361 aatccaagaa ctatctattc cttatcaaaa agaattgcaa tctggacgaa tccagtcaaa
    24421 gatcatccgt gatgtagaag ctatccaaac gttgtcctct caagtatttg tcagcggatt
    24481 aaatatcgct gtcaatttat tcgtagcttt aggtatcacg ctttataaaa gtcctgttgt
    24541 tttcatgttt ttcttattga ctgttcctct cgccgcattc gttactctct ttttccgtaa
    24601 aaggatcaga cagtccaact cagacttcag gcaagaaatg gaagagactt ctgcaaaagt
    24661 gatcgaagtg atcgaattag caccgattgc tcgagctcat gggttagaaa aacacgaaac
    24721 aactcgctta aataagcatt ttactcaaat ctatgaaaaa ggattccact tagatatcct
    24781 gcaatctgtt ttcggatcag tcagctgggc tagttttcag atatttcagc tgatttgttt
    24841 gattttcact gggatattag ctttacaagg gcgcatccaa cccggcgatg tcgtgatgta
    24901 tcagacgtat ttcacgacga tcgtcaattc cgtttctgga atgatcacgc tgattcctac
    24961 gatttcaaaa ggcatggaat ctatcacttc tattggcgaa ctgttgattg ctggcgatgt
    25021 agaaacttat cacggtaaga aaaaaatcca acatttggat ggaaattttc gttttgagga
    25081 tgtttctttt ctttatccgg actcactcaa accgattatc gatcattttt ctctagaggt
    25141 gaaagctgga gaaacaattg cttttgttgg gccatctggc tctggaaaat ctacactcct
    25201 taacttattg attggattta tccagccgac ttcaggaaaa atttttctag atgatcagcc
    25261 tttggatgaa ctggatatgc gaagtgtgcg aaattattta gccgttgttc ctcaaaccac
    25321 tttgttgttc tccgcttcga tcaaagagaa catcacttat ggcttgaaaa acgtatcaaa
    25381 agaacgttta gatgaagtca tcgaagctgc gcaactttct tcactgattg accagcttcc
    25441 tgatggtctg gataccttag taggcgaaca tggcaacaag ctgtctggtg gccaaaagca
    25501 acgtatctcc attgcacgag cgttgatacg tcagccaaaa atcattttgc tagatgaagc
    25561 aacctcagct ttagataacc agtcagagaa aaaaatccag caagccctga attacctgac
    25621 agaaacaccc actactttta ttgtggctca tcggctttca acgatcaaag aggcagataa
    25681 aatcgttgtg atcgatgaag gaaaaatcgt tgaaataggt acttacgaaa aactgttagc
    25741 tcaaaaaggc tatttctacc gcttgaaaaa tgaatgacaa aaaataggag ctatgacaag
    25801 acttttgtcg tagctcctat ttctgaataa acggtgttct cctgccaact cctcggtcac
    25861 aagtcacaat agtcatcaaa catgagaagc tgttttttga aattgttcct tgtaactcag
    25921 agttaaacgg taggctcacg acctcttggt aaatggtgtt caagagacag cctatcggca
    25981 ataagaagca attttgtcat cgctcaaggc cgaacgtctc tttcacaccc tctgttgttt
    26041 cataaaatag ttttattcgc tgtaaaggtc gcgaactggt gtcatgatga tacggttcaa
    26101 gtcattgatg attgtactga agccttgctc tttttgcatc aagtcattga ttacttcttc
    26161 tgattggatt ttcacagcca tttcttgtgc tttcattgca tcttcgtcgc tgaactcttg
    26221 tccactcatt tgtttttctt gtaattcgat ttgcatcgct tggaagtctt tgaacagttt
    26281 gtgtgcttct tcgtttgctt gtactttctc gtacgcttct ttcaacgctt tgaattctgg
    26341 taattcacgg atctcacgtt cgatttgatt cgcgctatcg tagatgtttg acatgttatg
    26401 ccctcttttc ttttagtagt tactctctat tgtaacatgg ggttattcgt tttcaaaggg
    26461 gctactctcc ttgatatttc cgccagaggt ctttagccgc ttcaccaatt ccctgaacgc
    26521 cttctttcac catatcgccg aagtttttca gcccttcttt tgcttgttca ccatattgat
    26581 tcaagttatc ttgccaattt ccagactctc cattagaatt tcctgtgttt ccaacttgat
    26641 ctgcagctac cacttgtcct cctgtggcat aggcatctgc tacactaaat cctgtttttg
    26701 aagtatacgg aaggatgcct tctgccgctg atttgaagac tttcccgaca acatttccac
    26761 ttgtcccttc caaatagtgc agcttgctgg attcttcgta acccatccat gtcgaaatca
    26821 ctacgtctgg cgtgtaaccg atcatccatt ggtcatttgc ttttgtggca tcgaaattag
    26881 tttctgtggt tcctgttttc cctgctatcg tataacctgc aggattcgca gcagccgctg
    26941 ttccatttga aaatgtacca agaagcatgc tggtcatttc atcggctgtt tcctttgata
    27001 tgacctgttt tggttttttg tttgtgttat cgacgatcac cgctccagtc gaatcaacga
    27061 ttttcgtgat aagatgcggc gtatacatct gaccatcatt ggcaaatgca ctgtaagcac
    27121 ccgccatgat caatggagat actccttttt ccagtccacc aagggctaag ccataatact
    27181 tatcagattt tgctaaaggc aggccgaatt cttctgcttt atcatatcct ttttgtaaac
    27241 cgatctcatg caaaagccat acagtaggca aattcaaact ttgtgcaaca gcttgataca
    27301 tcggtacttc tccttggtat gtcccgtcaa aattatgcgc atcgtaataa ctttgcggtt
    27361 cgtctttcaa gatactggaa ggcttgtatc cagcttccag tgctggtgta tatacgctta
    27421 atggtttgat cgttgatcct ggtgaacgac gcatctgtgt agcaaagctg aatcctctaa
    27481 agacatgatc tcctcttcgt cctactagtg cttgtacacc acctgtattc ggatctaaag
    27541 ctactgaagc agattggacc atggcaccat ctgaagcatt tgctgggaat aatgcactgt
    27601 tttgataagt agcttctaat tgcttttgat aatcctgatc taaagctgta tagattttat
    27661 agcctttatt catgacatct tcttcatcaa gaccatactc gttaactgct tcatcgatca
    27721 ctgcatcaaa ataataagga taacgataac cagaatctga attatcgtat gtgtcattaa
    27781 gatacgcgct gatatctgtc tggctttctg cttgttcttg ttcttttgtg atttttccgt
    27841 tttcttccat tacacttagt actgtgtttt tccgatcatt cgcatttttt gggtgatcga
    27901 tcggattata aataccagga cctttcagca ttcctgccaa agtagcagcc tcagacagcg
    27961 taacattcgt tgcatctact ccaaaatact ttctggaagc gtcttggaca ccccataccc
    28021 cgttgccaaa ataggcattg ttaagataca tggtcaagat ttcttctttg ctgtacttct
    28081 tttcgatctc gattgccaaa aataattcct tcgcttttcg atcaaacgtt tggtctaatg
    28141 tcagatacgc atttttggca agctgctgag tgatcgtact tcctccgccc cctgatgttc
    28201 cgccagacaa gaccattcgg actgctgcac gggcaatccc tttgatatca aacccgtgat
    28261 gctcgtaaaa attcctatct tctgtcgaga ttactgcatc ttggatatag ggagaaatcg
    28321 cgtttaattc tacatatgtt cctttttggg cgtacagtgt gccagcttct tcgtttttct
    28381 tatcataaat gactgtcgat tggcttaatc ctgattttaa cgtttcgaca tttgcttgtt
    28441 ttgctaaaat aaagaggtag atacttgtca ctaagacaca gatcaaacca atcagcaaaa
    28501 tcaatttgtt cacatgatat tttttccaga tgcgctttcg ccatcgatgg aattgtccga
    28561 gatatggtct gatccaacgc caaaaactga tcaaagcagc ttttatcttt tctaaaatgc
    28621 tttgaaaatc catttgcttc ttctcctgtc tatttgattc aatcgtctta ttttaccata
    28681 gtgaaagagt gttttcttcc tgcttaagtt gtagttcaat atgttttttt acgttagtgc
    28741 aagtagagta acataagaat gaacagaaaa atgtaagttt tttcttaaat atgttttaaa
    28801 aatggtacaa ttagtctgcg attgcactga aaggagtaca cttgtgaaaa tcacgatcac
    28861 tttacctatg aaccatcctg aaacaacaat tcgcgacttg cttgaaaagg aatggttagt
    28921 tcctagaaag gttcgacact ttttgcgtat acgtaaaaac gtttggatca atcaacaacc
    28981 tgctttgttc catcatgaag taaagggcaa tgaccagatc acattgttat tcgaagaaac
    29041 agattataat tatcaagaag ttcaattagg agatgcgaag cacatccgtg ttttatatga
    29101 agatgagcat ttgattatag tggataagcc tgcaggaatg aagactcatc caaatgaacc
    29161 gacagaaaac agtaccttac tcaatcattt agctgcttat ctcgacacta aacagcaacg
    29221 accttacgtt gtccaccgct tggacaaaga aacaagcggt gccgttttat ttgcaaaaaa
    29281 tccatttgtc ttgccgatac ttggaagaat gttggaacaa aaaatgatct atcggagata
    29341 tcaagccatc gtatggggag aaattgccaa agatatgacc atcaccaata aaattggacg
    29401 agatcgccat gatcggagaa aaagggtcgt agatgataaa aatggtcaga gcgccttgac
    29461 acatgtcact gtcaacaaag tactttctga acaaacacaa gtctattgtg ttttggaaac
    29521 cggacgtaca caccagatca gagttcattt agctcataag ggacatccga tcgttggaga
    29581 ccctctctac caaaatagaa cagctgaacg actgatgctc catgcattag aactgcacat
    29641 gatccatcca tttactcaag aaaaaatagt cggtaaagct ttacctggat tatgggaata
    29701 gaaataaaaa agaacagagt cttatcagca gataatgata agactctgtt ctttttgtgt
    29761 aatttgtctg cttacttttg atgttcgtgt tctgctaaca gcaaactacc gataatccca
    29821 ctttcattcg gcagtcccca acgaacgata tattcactta cttgaggtgt ttccatataa
    29881 ccacccatca attcaacaaa ttgctgacgg attttttgaa ggagatgatc ctgattcatt
    29941 accccaccgc ctaaaataat cttttcaggt gctaacgtca acgtatagtt gatcagtgct
    30001 tgagcaatat aataagcctg aatagcccaa acaggatgat cttgcggcag atcttgccct
    30061 ttgatccctg tgcgtgcctc aatagacggt cctgctgcta atccttctaa acaatcttta
    30121 tgataaggac aagtaccttc ataatggtca tctggatggc gtttcaccca aatatgtccc
    30181 atttcaggat gtgcgatccc tgaaaaaatt tcttcattga tcacgacacc tgcaccgatt
    30241 cctgttccca cggtaaggta aatacaacta ttttttcctt gtgctgctcc ttgcttcaat
    30301 tccccatatg cagcagcatt gacatccgtt gtccaagcag caggaatatt gtagcgttcc
    30361 ttgattgtgc caagaaaatc gaattgtgcc cacccttttt tgggagttgc caaaacgtat
    30421 ccgtaattat cttttgtttt gtcgatacca atcgggccga atgagccaat cccgattccc
    30481 ttcaattcat aacgatcaaa aaattcaaat acctgatcca atgtttcttc tggtgttgtg
    30541 gtagggatac ttaatttttc taaaatttct ccaccatctg aaaccgcaca cacaaatttt
    30601 gttccgcctg cttctattcc gccatacatt cgttttcctc ctcgaatccg attgtccgaa
    30661 tctcataagg ttggattatt gggacaaccg cttgcttcgt tggttcttca agcagattca
    30721 atatgacagt ttttccattg tctttagtta tttccaattt ttctaaatga ctagacatat
    30781 tgaaacctct tactactact ttatcactaa acttgctgcg tttcaaagct gtaattgcaa
    30841 acgtttcact ttttatcgtt aaatattgtt gttttgaaat caatgttcct tcatgatgtt
    30901 tgatttgttg agtggaaaaa ggaatctgtg ctgcgtatgc atgcaaataa gtagagaatt
    30961 tttcttctgg tccatgtagt tcaatgctgt aattgaaccg atgctcacct agacactgtg
    31021 cttctggtgt tggaaaatat ccccaatcgc ctagttctcc cacactgcga agtagcgtca
    31081 ctgcgatttg tccgtcttct gttacttcat attcattcaa accaaagttt ccgactgtta
    31141 caccgtactc ttcatcatga atattgacaa atgcttgctg atgttgcgga ttcgtggggt
    31201 tttcccaact tttagacaca tggtttgggc gcttcaccac ttcaaaaata ctatctgctt
    31261 cgtgatgttc cacatgtaac tttgtaggaa acaagacacg taagcgatga tctttcatct
    31321 gattgtcgag agtcgtttca aagtcgattt ttttactatc ttttctgatt gtcatgattg
    31381 tttttatttg aaggattcgt ttttctttgg atcgttctgc ttttcgataa cgaaattcca
    31441 caaccatctg ctgttctttt tctaaacgct cgtctgcaga aacagggatt tccatttcct
    31501 gtagaatact tatttttgct atttccggtg tatctactaa tacttttact tcagagtttt
    31561 ctttgttact ggataggatt ggctgatcac agaaaggttg tttaaagata tactcatttc
    31621 ctatatcgcc gatatcttca aaaatatgca gattattcaa ctttcgtttg tttttgcgat
    31681 cttccattgt aatggtacca tttttttcaa tcgtcaaatg tagagggccg ttttcgatga
    31741 ttcgtccact ctgatgaatc attgtttcct tttcttctgc tttcgcttcc ccttctacaa
    31801 gcgcgaatgt ttcccaagaa aatgctggca tgttttctaa tggtaaagtc actttgatca
    31861 ttcttgccat atatggcaca cggaaacggt ctttcggcaa atcataacca aattggacag
    31921 tttcttcaga taaaatagct ggtatagtgg ccccactttt tgtttctaca tgatacttac
    31981 gtttaggcaa attttctaac tcttgatata gttgttcagg tattccttca gcgaatcttt
    32041 ttctttctaa ttcgatggtt atttctgctt caccagtttt atccattcct gcagtattta
    32101 caatgacaaa tggaaagctg tcttttggga atccggtcgt atcgattgct gcggtcagct
    32161 gttctaagga atcttgtgca agatactttc caacttcatc tgctttttca aaacgaggta
    32221 tcatttcccg atggacagaa tcgactgaac aaccgcaaat actgtcgtga ggatgatttt
    32281 gcattaatgt cttccaagca taatctaatt gatcatgggg atagtttcca ctaacttcat
    32341 aagccatcgt cgcaagtggt tctgtaatat tttctagctg tcgctggacc ttggtattcc
    32401 attgttttaa gtaaacccga gcagatgctg tattggctaa agtataccag ccatccgttt
    32461 cttgacttgt caattctcct tctactgagc ctaagtcttc tggcacatct ttttgaactg
    32521 cttccagata gtcagtaaaa ttagaatgaa taaactcgta atctggaaac agttcatttg
    32581 cgagatggat tgcttttgat atatcttttt gtaccggttg atgatcaaca ccattcatca
    32641 tcaataagtg gttcgtagag gcatattgtt ctgcatccgc taatttttgc ttccaaaagg
    32701 caagagctgc ttctttttct actggaatct cattcccgtt gctataccaa ttggcaaata
    32761 gcagaccaaa gattgctgtt tgatcaggtc ctttccacca catctctgag tattgggaag
    32821 aatagttttc tgcttccaaa acttgattat caaagccaat tggttttaca ccacggccaa
    32881 acgctgctgc tgaaatacca gcttgtttca ttaattgagg cgtctgtccc atatttccaa
    32941 atgtatcggg aaaatagcct aacatgacag gggttcccca tttacggctt tcttccatgc
    33001 caatcagcat atttctcacg ttcgattccg agctgataag aaaatcatct tgcaagatat
    33061 aaaatggtcc aattctcaat tttccttcat tgattgcttg ttggattgcc tgtctttttt
    33121 ctggccttac ttgaagatag tcatctaaaa taatcgtttg cccatccaaa tgaaaactgt
    33181 tgaaggacgg atcaacttca aatagctcta gtagatcgtc tatcaattca atcagtcgca
    33241 tgtgatgctg ttcgtaagcc atgtaccatt cacgatccca gtggctatga gaaatgatat
    33301 atactttctt tttcatttac gatacctcct acttttctac tctaatatca aagtaatcca
    33361 tgactaattc gcaaaacatc atatttgccc aagaaaacca ttctctcgta taattttcag
    33421 gattgttcac atcgaatcct tcgtgcataa ggtgtgttcc tgcgtcagtt gctacaagta
    33481 aattcaatat ccgctctttt tcagatttgt ctgacgttgt cattccttcc atagctaatg
    33541 caatcggcca aacgtatttt tctggcgtat gggaggatcc aattcctttt gcgtactttc
    33601 cttcatagaa atatggattt tctttactca acaatgttct tcttgtttgc agataatctt
    33661 cctcttgttc agaacaatat cccaagtaag gtgcggcgat cagattgggc acattactgt
    33721 catccatgac tgtggcgttt cccattccat ctacttcata tgcataaatc gtttgccctt
    33781 tttgattaac cgttcgtccg tgattctgaa tcccctcttg gatctctttc ttcaatttct
    33841 gtgtttgctg tacaatttct ttatcatcga gtatcgtttg aaaaatttct tccacataac
    33901 ctaacacgac caccgcaaac atattggatg gtaccagata tccataacgg caagtatcat
    33961 cgctaggacg gaaaccagac catgtcattc ctgtaaaagc tgtttttgca cctcgcccgt
    34021 cattgatcag cgtatcttct tttcttgtcg tgtctcgtac aaatgtataa ggagagtgtt
    34081 catgatcttg ctctgttttg aacacgtcca agattttttt gataccttca ataaaagatt
    34141 gattgaattg atcagttttt cctgtatttt tgtaaagaag ataggccaac tgtattgggt
    34201 agcataaaga atcaatttca tattttcttt cccagatcca atcgttcatt tttgtatgat
    34261 ctgtttggtg gccagcaccg ttagcttctt cgttgaaggc attcgcatat ggatcgatat
    34321 tgatataaaa aaattgcttt ttgactaacc cgctgatcat gtcagctaat tcctcatctt
    34381 cttttgcaat cactaaataa ggacgaactt gtgcagtaga gtctcttaac cacatcgctg
    34441 gaatgtctcc cgtcaataaa aaggtggttc cgtcattttg ctttttgact gttgtcaaca
    34501 gagtattggc aaatgctgct gtaaaattct ttgcccagtc cgcatggata tccccacatt
    34561 tttgtgtgat atcttccata aatttctgca ccgattttgg tatatcttta taagccacta
    34621 ataaaacctt ctttctattt gatatatctt taaagattat acttcataca gccaattatg
    34681 tctacgcttt caaaaaatat ctttttattt actttttcat ccattatggt attatttgaa
    34741 tatgatatat cattaagagg tgtttatatg aatcaaccat tgtaccagac aatatttaat
    34801 gatttgcaaa cggctatttt aactggtgaa ttacctgtag acagccaact accaactgaa
    34861 aaagaacttt cacgtaccta taaagtaagc agaatcactt ccaaacgtgc attgactgaa
    34921 ttagagcaat taggacttat ttatcgcgtt cgcgggaaag gcagtttcgt taaatccgct
    34981 tctccggaac agattcttgc ttctcctaca aaaacacgac ggattctgtt tcttcttcca
    35041 tatgtcgctg atttgtctgt tggtgatttt agcaagggat tgaaccctgt gatgcaagat
    35101 aaacagattg atgtcatgat gacgacgctt gactttctac aaaataaaac agcaaaagag
    35161 ctaatcaatg aatttgacgg attgatttat tatgctcttg atacagaaca acatcttgac
    35221 ttactttttg agctttcatt gaaagaattc cctgtcgtca tattagataa aaaaatctat
    35281 gagttacctt ttcctaccgt tctttccgat aatttccaag gcggtgcgct tgctgcctct
    35341 tatttgatcg aacagggaca ttcaaaaatc gcttatttgt ttggtacgca gactcatcca
    35401 caatccgttc gacagcgtta ccttggttac cttgaagcat tggacaaagc tggtttgact
    35461 ttccacactt cactggatga caaaagcgcg acattagatg aattgctttc ttatatcact
    35521 aagtatgaag taactgcttt tgtttgtgaa aacgatgttg tcgctatcca gaccatgaat
    35581 ctcttgagac agcatggtta tcatatccct gaagattttt caattatcgg atttgatgat
    35641 atccaagcag cagctcttgt tgacccgcca ctgacaacca tcgcacaaga ttttgaacag
    35701 ttaggtaagc ttgcgggaga tgctttggtc acttggttag agacaaaaga tcgaccatcc
    35761 gatagtaaat ttccagtttc attcatcaaa agacaatcaa caaaggagaa ataaaatgaa
    35821 tatcggtcaa atagatacta gacatgcaac agcgaatcag ccaaatttct ctaatgggaa
    35881 ctgccagcct tatactggcg ttccttttgg gatgaattat tttgcaccgc aaactacgga
    35941 tcaaaacggc agctggtggt ttcatcctga tgaccgcgtt tttcaaggct accgtcttac
    36001 ccatcaacca agtccttgga tgggtgattt cagtcatatg ctgtttacgc cgatcaatgg
    36061 aaaactacag gagaacaccc ttttccatgc tcaaagttct taccgcccag aagaaagtat
    36121 attttgcccg actcatctgt cgatctgtca attacgttat ggcatctgtt ctacattgat
    36181 acctagcatg tatggcggaa tcttatctat tgattacagc aaaaatgaca gcggtttact
    36241 tttatccttt ccgggacgcc atcagctctt tgttatagat ccttacacag tggaaggaaa
    36301 aatcacacat tttgctggtt cagaagatcc tgatttttct ttttatttta tattgagatt
    36361 tgaatatcct ttaacaattg atgagttagc tgtttccaac aaaaatagcg actcgatccc
    36421 gttgtatttt gaacagacaa aaaaacaaac tatccgattt gggacatctt ttatcagctc
    36481 ggaacaagca gcttataacc tggaactaga acaaaaccaa agcaaagaga cttaccttgc
    36541 tcacagtcaa gaacaatggc agtcctattt tgatcgtata cagctcacac accatgatca
    36601 acaacaattg cggacgtttt atcataattt atatcgggtg tttttatttc cacaaacatt
    36661 ttatgaaatt gatcctagtg ggcaaaaaat ccactatgat acgactagtc gatctataaa
    36721 acctggtgtt ctctatacaa ataatgggtt ttgggatacg tataaaacgg tttatcctct
    36781 ttattctttg attgctgtag aaaaatacga agaaatgctg gaaggttttt tgaacagcta
    36841 tagagaaagt ggctatttgc caaaatggct gtctcctgat gaacgtggac ttatgcctgg
    36901 gacattgatc gatgcggtca ttgcagatgc tgctgtaaaa gatatccgac gagacttgat
    36961 gcctgaattt ctaacggcaa tggaaaaagg tgctacggtt caaagcaaac atccaaatta
    37021 tggacgccaa ggaacagaag attacttgac atatggctat gttcctaatc actaccacga
    37081 atcagtcaat cacacattag actatgctta tagcgatttt tgtattagcc aagttgccaa
    37141 ggtactagaa gaagaggaaa tgacgaagaa atatcagcag caatcattga actatcaaaa
    37201 tatctttgac tatgagtcag gatttatgcg ttcaaaagat aaagatggca agttcagaga
    37261 accattttca gctaatcgtt ggggacaaga ttatgcagaa ggaagtgctt ggcaaagcag
    37321 ctttgctgtt tatcacgatt tttcaggact gatcggaaaa tatggtggca gtgagagata
    37381 taaagaaaaa ctcattgagc tgtgcaatca gcccccaacg tttgacccag gaggatatgg
    37441 ttttgagatc cacgaaatga gtgagatggc cgcgatagaa tttggacaat tagctatttc
    37501 aaaccagcca agttttcact atccttactt gttcagttat ataggacaac ctgaaatggc
    37561 acagccactg cttaaacagt tgatgactca gaccttcgac gattctccaa tgggttaccc
    37621 tggagatgag gacaatggaa gtatggccgc ttggtatatc ttcaatagtc tgggatttta
    37681 tcctgtcacg cctggaaccg gtgaatatgt tatcggtatg ccattaattg acaaggctgt
    37741 tttgcttttg tcaaatggaa aggaattgac gattgaagca acgcctaatc ggccgcagca
    37801 acaatttatc cataaaatca aacggatgga caaagaatat acgaaattat attttaccca
    37861 tcaagatttg ctgaaaggcg gcacgatctc ttaccaatta gggatcatcc ctcaccctaa
    37921 gaaatatcaa gcaaaagatt tgcctttttc cttatcaaac tataattaac aagaggttgt
    37981 gaaaaagctg tcctgcatca agtaataaga acagccaagc aaaaatagat cctcatattt
    38041 ttcgtttggt tggtcttatt accgcagatg caagcttttg aacaccgttt attgagaggt
    38101 tgtgaaatca gcgttctgac ctgagtatta gagagacaaa tggcaaaata gttcctcata
    38161 ttttgccatt ttttgagcta ataccgaggg tcagctgatt gaacaccgtt tagtggagag
    38221 gtcgtgaaaa agctgtttag tttcgagtat taagaaaaat tttgtgaaaa tggctttcca
    38281 catttttgca taaatttggc ttaataccga gaaactggct tttgaactcc gtttatcgag
    38341 aggtcatgaa aaagctgcct tgcctcaagt aataagaaca gcccaaaatt agatttctag
    38401 acaatttttt ataagttatt tcattacatt ggctttttct tttatgattc tccactgttt
    38461 tctcggtact ctttagggga tctgccgacg attttcttga ataatttaga gaaatacgtg
    38521 gtgttgttgt agccaatctc acttgctatc tcattgatgt tttgattact gtgcaaaagc
    38581 agttgtttcg ctttttccat acgtaaatgg ttcaaataag ctgaaaaact ttttttcgtt
    38641 tcttttttga acagctgtcc tagatacatg acatttaaat gcaggttttc tgacacttct
    38701 ttgagtgtaa gtggttcttg ataatgctgg tgcaggatta cgattacttt ttccacattg
    38761 tcactgtact ttttctgtct ctgatgcttt ttgatgaatg acagaagtag ctgctgaagt
    38821 tcttgcaccg tttttgcctg atggattttt tcgattcctt gtaaatattc ctcatcttcc
    38881 agatcaatca attcccgttg aatctccata aacagtaaaa aactggaata tcgtatatct
    38941 tccggcatca tggctgctaa ctgaaactgt tcaaaaaaat cagtgatcat tttttgagcg
    39001 acttccaatt gtttattttg cagcacgcgt ctgaaacttg aaaaatcgat ggtttgatct
    39061 tttgtttctg cttgtactgc gtaaaaaaca tgctcgtctt tatctccata aaattgatgc
    39121 aactgtagat tgtctttcgc ttgttggaat gtttcaggga ttttgtccaa agaatctgtc
    39181 tcttccccga tactgatcag ccattcctga tcaggaaagt tttcttgaac aaaacaacag
    39241 aagccatcaa cttcatctgc ctccaacaac aacgtgatca atacacggtc tccgtaattt
    39301 cgttgatagt aaaagatttg ttgccgttct tgcaaccatc tattgataag agtatcatga
    39361 gttcttgcca gttgaatcaa tactatccta tgctgcttct cacttatttg tcctattatt
    39421 tcttcttcac ttatttcatc caattcttca ttcaaccatt gactgaataa cagttcttga
    39481 tagatttctt gttgattttt ttgttcattt tgctgatcta accgtgtttt gattttcttg
    39541 aggctttcaa ccaattcgaa cttattcacg ggtttcatca aataattcac ggcgcctaat
    39601 tgcatacctc ctttcaaata atcgaattct tggtatcctg ataaaatcat aaattcaaaa
    39661 ttatgacgtt ctttttgagc cgcttcaata aattccagtc catttatttc tggcattgtc
    39721 acatccgtta ctactaaatc aatctgtcgc tgttcaagta cagctagtcc ttctttggca
    39781 ttctctgctg ttgctactat ttgaaagcct aaactttgcc aatcaattat cttttttaac
    39841 ccattcaaaa tcatgtattc atcatctacc aataacactt gatacacgct catccctcct
    39901 tgaatgtaat agaaacggtc agtccacctg tctcattatt ctcaagcgac atataataac
    39961 tgctgccgaa gctggcacgt aaacgttcat tcacgttttg cagaccgata gaaccgtgaa
    40021 gttccacttt aggatgagac agtttttctt caatctgttt tagccgtttt tcagtcatcc
    40081 cttttccatt atctttgatg atgatcctta cccgtttgcc ttcctgcaag actttgacac
    40141 ttagcgcatt atcgaatcga gtaaaatcaa taccgtgttt aaaataattt tcaactaatg
    40201 gctggatcac aaatttaggt acttcgatct tttccaaatc aggatcgatc ataaaatgat
    40261 aggctactcg attaggataa cgcatttgat acaaatagac atatttttcg caaaagtcca
    40321 gttcttcttt taaagtgatc gttttctctt gattcgtatt gtttcgaagc aaagcggaaa
    40381 acgcatacac aacatctgct aattcttcac ttccttcgct gattgcatac attcggatat
    40441 attctaatgt attatataaa aagtgtggat tgatctgtgc ttgaagcgcc cgcatatgtg
    40501 cgtcttgctg tttgatttct aatttgtaga tgtcttctac aaactgattg atgctatcca
    40561 acatttcgtt gataccgatt gaaagttcct tcagttcata ttccgtgttt gttatgtcga
    40621 tccgcgtctc taaattccct gctgctgttt gatccatggc ctgcatgacg atagatactt
    40681 gctgtgaata tcgtttgaat gttttataaa gaaagataag cagtatattg tctaaaataa
    40741 tgataccaat gatcagcggg gcaagatttt ttcccgtttc gatttttaca ctttttttat
    40801 ctagtacagc taatatacga taatcatcag acagttcttg gtgttgaaca aaattgtttt
    40861 tttccagatc gtcaatcggc aattgtgaat ttttcttcag actctctgtg atgatcattt
    40921 cttgtctttc aaacgtttct ttagctattt tattatcatg gaatgtgtat agtcgatttg
    40981 tcgttccaga aatcatgtat aaagagagtc cattgaatga cgttagattt tctaaaatca
    41041 tatctagttc tttcttttga aaaccaataa aaatacttcc tgactcagct cctccttgtg
    41101 tgataggcaa cttgatataa aaggcgtcta gtaattttgg tgtccccttt tttatttccc
    41161 cacctttgtt ttctctagaa gattcataat actccggtag ttgattcaac acgatatata
    41221 tcgcacttag ttcttcataa tttgcgtaga gatctttgat ttgatttggg aaagaaacta
    41281 attcatcggt actgttttgc tgttcgtata catacatcag atactgatct atgggttttg
    41341 ttaagtaggt cgtcacattt tcgatctttt cggaattccc tacaagctgg tttttgatga
    41401 tcctactcaa ggttgtttta tcattcagca ttctgcttgt gctttgaacg gcatcacttg
    41461 tcatgttttc tgcctgtttt tgattttgtt cccaagtatg gatactaaaa ataacggtag
    41521 cagtcatcgt tacaccgatc atgataaccg cgtatttccg caacagacga tttagtaagg
    41581 ttccttttga aaatagaatc cgaaacttat tcataacttt ccaactgttt atctaattct
    41641 tcacgccaat gtgttgtcat cgtaccattc caaaccgtca aaagaccaat gacaccaaac
    41701 aggatcaatc ctttgaattg ccaagtcacg cctaaaatta taccgctacc aataattatt
    41761 tttagaaaag acgaaaagct ggaaaaaacc gaaataaaac ttagctttag cagattagga
    41821 acagagatcg tatattcact atctaaaatc atactgtatt gataagtagc aaataggcag
    41881 agcaaggcaa aaagtaaaat aaaatcaata gctaaaaaaa tgagcccttt gatttgaaca
    41941 gataaatata aattgtaact caataaagca gtaccagata aaaataaact aaatagcaga
    42001 ttgcctctta aaaaatttct tttatatata ttccagcctc tttttattgt gatttcttta
    42061 tactcgaatc cgtacaaata aaatagttca ttgactgttt tccatgccgc acctaatcca
    42121 aagatgatcc cgccggcaaa tgtatatatc cagaaataca aggttagttt tataatgacc
    42181 cagatccgga taaataactc ttccaaaacc ttccctaaca tatcttctcc tccaaacatt
    42241 aagtttatac tttctcatta tacaaaaaaa ggttgggcaa tcagcacctt gacctgatta
    42301 ttagagaaac aaacggaaaa atagttcctc atattttgtc gttttgtgag cgaataccga
    42361 gggttagctg attgaacacc gtttaccaag agtttgtgaa atagccgctt agtttcgagt
    42421 agtaagaaaa attttgtgaa tatggctttc cacattttta catatattta gcttaatacc
    42481 gagaaactgg cttttgaaca ccgtttattc ggaacaagag gttgtgacac gtcttttgtc
    42541 acaacccctt tgtctttatt cattattatt cttgtcggaa ctcatcatat tgtttttgca
    42601 tttctttcaa taccttatca tagccagcct tgtccaaagc gtctttcaat ttcggtaatg
    42661 tttcatctgg atcaacagtc cctgtattca atccatctaa atattgattc atgacattag
    42721 aaatattact taattctgtt ttgacactgt cagtgttgaa gctgaatcct aaaatcggtg
    42781 aagtttctgc atcagcaatc gattgatcac gtttggcaat catgtcatcc gtaatagact
    42841 cttgtgtata tagaatcttg ttgtttcctg tattccaagc agacatatgt gtatttggct
    42901 gataaccatc tagcaatttg attttatcat ctgaaccatc gactttttcc catgcttctc
    42961 cctcgatccc ccaaacaagc ccattcaata attctggatc actgttaagc agccctagaa
    43021 gttctactga tttttcttta ttttttgaag tatttgatac gacaaaattt gccatttgtg
    43081 cttgagctgt agattttagt ggaacagtaa ttgcttttga gacaagttct tgttttgctg
    43141 catttgtcaa aattgtatcg ccataatcat acggtccttg tgtttctcca cgcattagcc
    43201 aagtatttcc ttctagtgga taatccttat tgctcgttgc agcgtcactt ggaatgagtc
    43261 cttgtttgta ccagctatgc attgtacgta acagtccgat atacgtttca ttatcgtatt
    43321 ggttgatgat tttcgaatca tctccatcca gatcaatagc aaatggtaac gtattcccta
    43381 aaggataatc aaagttccct tggactttga agccttgtcc aatggcaaaa gctgccgtgt
    43441 ttggttcttt ttcatggaat tccttcaaga ctttttctgc atcagcgtaa gattggattc
    43501 catcaattga aagatcgtat ttatctagta aagctttgtt aaaagtaagt acttgttgag
    43561 caaagacatt tccattgact gggaaagcat ataattttcc attgaccaga tttcctttaa
    43621 tatatgcttc atctaaatca ttataagctt tttcagcgta cttaggcgct aattcagtca
    43681 aatcagcaaa ggctcctttt tgggcattgg aaacatagtt gttcgcaaag gcgatatcat
    43741 aattttcccc ggaagaaatg atgacgttca ttttcttttc gtaatcgccc cagccaatgt
    43801 aattgatatt gacttttaca ccgatctctt tttcgattcg tttgtttgct acttccatca
    43861 attgatcgaa attatctggt ttatctccga tttgatacat ctgcaaagtg tccgaattgc
    43921 tagcttctgc ctggtcttta gaaccagaac ctccgcatgc tgctaatgtt cccaacaaca
    43981 acgctgaaac acctgtaaag gctagtttct tccataactt catttttctt cctcctaatt
    44041 ttatgattta tatttttgtt tctttattct tttactccgc cgattgttag acctttgaca
    44101 aaatgcttct ggaaaaatgg atagctgatc gcaataggca acgtcgagat gactacgatc
    44161 gccattctgg cagattctcc tggaactgag gctaatccgc ctgtcaattg gcttcctgct
    44221 cctgaatttt gtgtcagata ttgaatatta ctttggattt tcatcaaaag atactgtagt
    44281 ggaactagct tatcatcttg gatgtatagt aaagcattga accagtcatt ccagtaaccc
    44341 aatgcggcaa ataaactgat tgtcgcaata cctggaacag ctaacggtaa aacgatctga
    44401 acaaatattc gcagttcgct agctccatcg atccgtgccg attcaataat cgaatcagag
    44461 accgaacgtt taaagaatgt gcgcatcaca ataatattga agggactgac agccattggc
    44521 aagatcaatg cccaaatcgt atcttttaac tgaagaagat ttgtcataac aagataattg
    44581 gccaccatcc ctggtgagaa caacatcgta atcaaacaaa aaacggtaaa aaaacgacgg
    44641 aatggaaagt ttgaccgtga aatcgcataa gcatataaag aagtagccgt gctattgatc
    44701 agtgtaccta aaacagtaac aagtacagtg acgaaaagcg cttggaaaat tttatcctgc
    44761 atctggtcga acaagtacgt ataaccaaat atactgaact tttctggcca aaaactgtac
    44821 ccgtgagtcg acaatatgga ctcatcggta aaagagatgg cgataacaaa tataaatggc
    44881 aaaatacagg aaatagcaaa gagtgcaatc attatattag agaaaaaatt agccgtctta
    44941 ttgaaagagc ggacttggac tctctccact ttcttttttt tcattttttt cctccttaga
    45001 ataatgcaga atcgctatca aaacgtcggg cgatcgtatt tgtgatcatc aacaagacaa
    45061 agccgacaac tgattggtaa agtcctgcag cagccgtcat accaaaatct cctgtggatg
    45121 tcaggccatt gtagatatac gtatccaaaa cttgagtcac atcatataat gcaccggaat
    45181 tcctaggaat gttataaaac aggccaaaat cagctcggaa aatgtttcct acggctaaaa
    45241 tcgttaaaat cgtcattagt ggaataagtt gtggtatcgt cacatgtttg atctgctgcc
    45301 atttgctagc tccatcaacc atagcagctt cataataagt cggatcgatt cccataacag
    45361 atgcaaagta aataatgctg ttataaccaa tccctttcca tgtaccaatg aacaccaaga
    45421 taaacggcca atatttcggt tcattatacc aattgacagc ttctcctcca tgtccagtga
    45481 tccactgatt gaatattcct ttgtcaggac ttaaaaaagc gtaaacaaag taactgataa
    45541 tgacccaaga tagaaaataa ggtagtaagg acatcgtttg ataaacttta acgagtcgtt
    45601 tatttctcaa ctcgctcata atgattgcaa atacgactga gatcagcaga ttcaaaagga
    45661 taaatgctac attatataaa attgtatttt tagtgatgag aaatgcttga tccgaagaaa
    45721 ataaaaattt gaaattttcc caacccaccc aaggactttc cctcaagcta gccaagaacc
    45781 catctgctga aatatgaaaa tttttgaatg ctacgacatt cgccattaca ggtatgtaaa
    45841 agaaaaaaat catccaaagc atacccggta cagccatcag gacaagtgct ttataacgcc
    45901 atagattcat aaaaaaatga ttgaacccct ttcgtttttt catctaaaaa cccctttcga
    45961 ttcattcgca acgtaattat agcgctttca ataatgagaa taaataaaca aagtataggg
    46021 aaaatgaaac aaagtatagg agaggttgtg aaatcagcgt tttggcctga gtattagaga
    46081 aaaaaacggg aaaatagttc ctcatatttt gccgtttttt gagctaatac cgagggtcag
    46141 ctgattgaac accgtttatc agaggttgtg aattcagcgt tttggcctga gtattaggga
    46201 gatcgtgtcc ttactatcta actctgagtc acaaggaaca atggggactg taacaaaagt
    46261 cttgtcacag tcccttattc cgaacaaacg gtgttccaga agtagcttct acggaaataa
    46321 gccgaaattt cacaaaaaag ccatccgaaa gtcagacgga caagtctgct tttcagatgg
    46381 cgctacaact actctgctta ttcttttagc tatccattca attatttttc tctcacttgt
    46441 tctagctctt ctttggtcat gggtcgatat tgccctttat tcaaatcttt aggcagccaa
    46501 aattttccca tacggattct ttttaaatat aagacttctt ttcctactgc tttgaccatt
    46561 cgcttaactt ggtgaaactt tccttcatgc aaaatcagac ggataattga agtttctgtc
    46621 tcttgatcta cttgatcaat ttggagtttt gccggcaatg tctgctcatc gccatcaata
    46681 atcagtcctt ctgcaaataa acggatatct tcttctgtca ttataccgct aacctcagct
    46741 agatattctt tctccacatg ttttttaggt gatagtaact gatgggcaaa caccccgtca
    46801 ttcgttaaaa tcaagaggcc ctctgtgtct ttatctaatc ggccgactgg aaaaagatct
    46861 tcccgataat cctggtcttc caataaatcg attacggttt gatcatgatt atcctgagta
    46921 gcagaaatga ctcctgcagg tttatgcaaa atgtagtaaa aatctttttg atacgtaatg
    46981 atttctccta aataagtaac cgcatcttcg aattcattta cttgcgcttt atcgcttttc
    47041 accacttgtc cattgacttc gatcgttcct tttttgatta atcctttgac ttctttgcgg
    47101 ctgccaagtc ccatatctgc taaaaactta tctaaacgca tgcgtttccc ccatttcaaa
    47161 agagcatcaa tctagctctt ctgattcact tattttattt ttaatttatg tcgtaaacgc
    47221 gctattcctg gtcctagtag tctttctgcc aagcggattt tcaaagctaa ataaccatac
    47281 actaaagccc ctatacctgc tactacaaga atcaacaata gagactggaa tttgctatct
    47341 tcgtttaaaa aagcaccaaa aatcatcttt gtgatccacg aagccaaaag cataatcaag
    47401 gtaagaataa agatcagcaa tgtcctgcgc cagacaaaac ttggtttaaa gcgggctact
    47461 tcatggattc tctttaagat caaggtacaa gacacagcaa atccgatcat tgtcgccaat
    47521 aaagggccgt atacttcaaa taaacgaatg gctgggtatt gaagaaggag tttcactaga
    47581 aacccaataa ccaaataatt aattgccgcc ttgttttcaa acattccttg cagcatgttg
    47641 gaaactagca tatatagtcc taggaagagt cctacaaaac ttgcttggat cagtacatta
    47701 cttcctagag aatccggtgt ataaaataat gtatttagcg gataggcaag taacataaca
    47761 ccaaatgtcg ccggaaacat aaaaaatgaa aacaactgta aatttccgct tgtcaatttt
    47821 gccagaccta ctcgatcttt caacgtaaca gcttctgtaa tcaacggtaa cccagtagac
    47881 gcgatcgatg ttgccaatgc aataactacc atcgtcaact tatctggatt ggcactaaaa
    47941 atcgaaaaaa gatccagtaa ctgtgcattg gaatactcgg tggatgcaga catgatcttc
    48001 ataaaagtga actgatcgac taatttgaag atcgttaccc ctgaacccac aataataaac
    48061 ggaatcgctt ctttaacggt atctaacaac aattccttcg ttgcaatatt tattttgttt
    48121 tcgctgtagt gaataaacgc cgatgtatac gcttgatgtt tcttcaaaaa atacaaaagg
    48181 acaagaatac ttgctagcat accgataaat gccgcgaaag ttgattgcgt gaccgccgtc
    48241 acatagtcac catccatgac tttcatgatg atgaatgtag ccaatagcat ataaaaaata
    48301 cgtgccacct gttctacaat ctgcgacagc gcataaggca tcatttcttg attgccttgc
    48361 aaataccctc gtatcacact catacaagga aaaaccaata cagcgatact caaagaacgc
    48421 atcgttggaa tcagttcttc tccgccacca gaagcatgag ctagccaagg agaggcgatg
    48481 tacatgaaca aagcaaacaa caggcctaac ccgcccatca tctgtaatgc acggataaat
    48541 aatcgtcgtg aagtgccata ttcattcaat gaattatagc gtgcagtttg ttttgcaatc
    48601 gcagcaggta ttcctgctgt agagatcagt agaaacaatg catatatatt gtatcccata
    48661 ttgaataatc cattggcagc cttagcattt tcgcccatcc aagcatacca gggaatgata
    48721 tagacagcgc ctagcaatct tgaaatgata ttactggcgg tcatccaagc agagcctcgt
    48781 gccatttttt cttgcggtgt taattgatct aatgctggtt ttctttgatt atccatcggt
    48841 cagattccaa ctttctacaa aacattacta tcattttaat tggtaaaaca gaaactggca
    48901 atcgatttta tataactttt ataagaacaa ttgttttttt gaattcggtt tcgttttttt
    48961 ggtaaagtaa agaaatatac agccttttat ggctaacatt tataggagat atcaatgaca
    49021 caagaaataa aaaaactacc cgttatattt ttaggtcttt tctttatcac agttggattg
    49081 aattggttct tgctccctca cgatatagct tccgccggag tgggcgccat cggacattta
    49141 gttgaaacgg ccttttctat ccaacgctca gttactgttt ggagcatcaa tcttatgatg
    49201 ctgcttctag ccgctgtcct attaggaaaa tcagtatttt ttaagactgt agcgggaagt
    49261 ctgctttttc ctgttgtttt agagattgtt ccaaaagtgg aaatgatctc ctcacatttc
    49321 ttttcactga tttcaggaag tcttttgttc agtcttgggg tttacacatt gtacacagtc
    49381 ggggcctcaa atggcggtat aacgataccg ccgattattt tccacaaatt ttttcggctt
    49441 ccaatgccgc aaggactttt acttaccaat agtcttatcg tctttttgaa ttattatgtt
    49501 tttggattgt tggagacact attcgttctc ctatctatta cagtcagttc attgtttatg
    49561 aaaatactca tccgcttgtc ccctgtgtca agaaaatttg aataaagctg gattttgtca
    49621 tagcggaaac aaacgatata ttaaaaagag tgtgctataa taaaccaaga aattgaataa
    49681 agcgagggat caatcaatga gcttctcttt agaagacatc cgtcatttac tattaaaaga
    49741 aaatctatta aaagaatttg tttcaacaag aggctggcat cttgatgtac cagaaaaagc
    49801 tgaatttctt cagttaagtt atgattcaag aaaagctgat gcttctactt tgtttttctg
    49861 taaaggaatg aatttcaaag aagaatattt ggattcagca atcgaacagg gtattcaata
    49921 ttatgtatca gaacaaccat acgaaaattc agcagtagga atcatcgtaa cggatatccg
    49981 taaagcaatg gcgcttcttg ctatggcttt ttacgactat ccgcaaaata aattgaaagt
    50041 gatcggattc actggaacaa aaggtaaaac aactgctgcg tactttacaa aagcaatctt
    50101 ggatcacaca acaaataaaa aaacagcatt gctttctact atgaatacaa cgttagatgg
    50161 caaaacatat ttcaaatccc atttgacaac gcctgagtca ttagatttat atcgtatgat
    50221 gtcagaagca gttgaaaatg gtatgactca tctagtgatg gaagtttctt cacaagctta
    50281 caaaacacaa cgcgtatacg gcttgacttt agatgtcgga atcttcttga atatctcgcc
    50341 tgaccatatc agtccgatcg aacatccgac atttgatgac tatttctatt gcaagcgaca
    50401 attgatcttg aattcaaaaa cagttgtttt aaatcatgaa agtgactatt ttgatttact
    50461 tagggaaaca gccgatctat tccagattcc aacgatcact tatggtcgtt cggaagaaag
    50521 caattatcaa gtgatccgtt cagataaagg tactcacggt tttactttat cttctcatga
    50581 agatcgctta gctatctgcg atactgctta tgatattttg ctggctggag gattcaacca
    50641 agaaaatgca gccagtgcga tcattgccgc agcactaaca ggtgcctcca gtcaagatgc
    50701 acaagacgga ttaaaagaag ctcgtgtacc aggccgaatg gatcaattga tccaatccaa
    50761 tggagcgcat gtctatgtgg actatgcaca taattatcta agcttgaaaa cattgctgaa
    50821 atttgccaaa aatgaacatc cagatggccg tgtaattgtt gtattaggca gcccaggaaa
    50881 caaagcaatc tcccgtcgac atgattttgg aaaagtgcta tcagaaacag ctgacatcgc
    50941 ctttttgacg gcagatgacc cagcatttga agatccgaaa aaaatcgcgg aagaaatcaa
    51001 tgaagcaatt actaatccag atttgatcgt tcattatgaa atggatcgtc ctgaagcgat
    51061 tcgacgcgct ttagcagaaa gcgctccgca agattcagtc gttattgcgg gaaaaggcgt
    51121 ggatccctat caaaaaatca atggtgtaga tgaaccttat gaaggggatt atgcaatcgt
    51181 gaagcggttg attgaagaat aatggtattt atatactagt ggtttcccat ttctaagaaa
    51241 aaaagggact gtgatttttt gtcacagttc cttttttctt agaaatcatt ttaacttttc
    51301 actagctaat tctgctgcct tttctctttc ttcatttaat aatttattat catagaattt
    51361 gataaatggg aaccagataa tgaatgcgat gattgcacaa atgactgcta acacagcagc
    51421 tttccaatca ccaccactac caacaaatgc tccgatcccg actggtgaag gccacggcat
    51481 ctgtgcaagc atcggtttga cgatttccag ccgaatggca aaataagcga gtgtagcaga
    51541 agccatcgga gctaagaaaa acggaaccgc taaataggga ttgtagacga ttggcatccc
    51601 gaaaatgatt ggttcattga tattgaataa tcctggtaca agtgaggctt ttcctaacac
    51661 tttcagctga tcagatttcg cgaggaatgc aataaagatg atcaatccta aagtcgcacc
    51721 tgatccacca acggttacat aagaactgtt gaattctcct gccaacggat aatttgcgcc
    51781 ttgtgcgtta gctgccatat tagctaatac gatcggggtc aagaaagaag tgatgatatt
    51841 cgctccatgg atccctacga tccacaatgc atggatcaag aaataaatca ccatgacacc
    51901 aagccatgta ttcgtcaact gcgtaacaaa cccaaatggg atagcaatca ttttgaagat
    51961 atccgttcct agtgcaacta ataatccatt gatgatcatc accacaaacg caatgacaaa
    52021 tgttgggatc agagcagtga aagaacgaga aactcctggc ggaacaacat ctggcatttt
    52081 tatgacccaa tttctcttga cacacataca atacaattcg accgcaataa tcgacataat
    52141 aatagctgta aagattcctg aagttcctaa acgttcgaca aaagagccca tcctaatacc
    52201 atttactaca gtttctgtct cattgataga agcaatcaag ctgattttac catcagtcat
    52261 gatcaattca gggatacaca taaagaatgc aaaaacagat agtaatgccc ccgtgagcgg
    52321 attaacattt aatttttgtt catctcgttc aattgacgtt aattcataag caaacacaat
    52381 ggcaaagtaa atagataaaa tccccatcgt cattgtattg acgatcatgt acaagtcact
    52441 aactttgaaa aaagttgcat taaaaaatcc ttctaaaaat gggaacggta atggcaaaat
    52501 atttaaaacc aaaaacatcg agcctacaat cgtgaaagga atagaagcca tccccgctgc
    52561 catgacagcc cgaacgattt taagttgcgc aattttcccc ataggtccca tcaggtattt
    52621 ctgcaaaaaa tcaaacatta gttattctcc tttcctaaat ataacgcttt ttcatattgt
    52681 ttaatacgga tatactgttg gtcttggttg agtgaaattt tctttaaacg atactgtata
    52741 aatgcactgg caaaaatacc gcttacaaca agaatgaaaa caaataactg tcctccattg
    52801 ttatttgcca taaaaggata caagaatgta aatcctgagg taaacaacac tggaatcact
    52861 aataaattaa agacactctg gaacaaaaaa taccactttg tccatggaac gttcgtttgg
    52921 tgttttcgat aaagctttac ttgttctcct actgcaggca aaatagctaa aatcagaatc
    52981 atcggtaaac tacttcctaa acttttagct gatagaagca gaatcatcca ttgcatgttc
    53041 atgaaaaaga aaaaggcagt caggtaacgt acgattagat aacgattgaa atacatgttt
    53101 ttcaaactaa gttgcttttt ttctaattcg attttccttt ttactgtatc ctgcacaatg
    53161 atccttcctt tctatttatt tttttgatac aaataattca tttcctgtgc aacttccaat
    53221 aatgtcatcg tcgtcatcag atgatcttgt gcatggacca tgataatttc catttctatt
    53281 tttgtcccgc tggcatattc tttcagaagt tgagtttgtg actgatgcgc atgaagaatc
    53341 tcttcatttg cttgtcgcaa cttctcttct acttcttcaa acatctcttt acgcatcgag
    53401 cgaaatgctt catgtatcag tgtccgtgca tttccgctat ggaaaatgat atcaaaagaa
    53461 gtcacttgta gtgtttcact gtttattttt tcagccatct gttttcctcc ttattcgatc
    53521 agtttgataa atttactgct gaaattctca aaaataggtt cagctaagat ttcctgctgt
    53581 acttttagct ggtcaactaa cctaacgatt gctttagtca cgaccgtaat tccttcattt
    53641 tcgacataag aaggcgaaac taaaaaaaca aaatgaattt cttgatattg atcccactgc
    53701 atcccagaag gaatcaatgc aacggctacc tttgtttcga ttccaactgg aacagcggga
    53761 tgtgggactg ccactgtatc actgaatatg atttgtccca tattttcacg ttgctggatc
    53821 tgctttcgca tttctcttat atatccttct tcttcattta tgctcagctt ttgcagcaaa
    53881 tcatgaagaa tttcttcctt tttcggttcg tacgtataag tcttgaaaaa tttttcagac
    53941 aactgttcgg tgtaaatctg ctttttttgc tgtagagaaa atgctggtat cggtctatct
    54001 gaggaatgat agggtataga ttctccaact acctttcgta ttttacgaac atcatcttca
    54061 ttcaaaaaaa tactgacatg taaaactgga attttgaaaa acattgtcga aagatcaata
    54121 gaagaaataa tcaggtcgat tccttttaaa gttgtttcat tgatctcata atagcctttt
    54181 acacttacca ctgtaatatt tttaccgaat tcactgacta cgcgattctt taatagttgg
    54241 gcactgccgt aacccgttgc acagataatc aatgcatgta gtttatgcgc agctctttct
    54301 ttttccaatg ccgccatgag atgcaaagcc aaataggccc attcatcttc attgatggta
    54361 tagtttttca aagccggcat gttgctgaaa tgccttttta ctgcgtcaaa gactttggga
    54421 ttttctttct ttatctcctc agtcaatggg ttctcaatgc gaatacctcg gctcaatcga
    54481 atcatcatcg gtgacaaatg atcgagtagt ccattgaaaa gctggagatc ctctactaat
    54541 gaaaaatgaa attcctgaga caatcttttc agaactttcg ctaattcctt tgatagttcc
    54601 tgatcatttt gattttcttt gtgattcgac ttagccatga gatgtaatgt caaataagca
    54661 atttcttctt cgggaaacga tacacctgcg attacctcaa tacgttgaac gatccgctgc
    54721 gccacttgat actctactcg cttagaaact tcttgttcga tgcctaattc cttgatttca
    54781 aacccttctc ggagccgttt gatccccaaa gccaagtgta aagtcagatt ctgtatgata
    54841 aaatccgaaa cttttaactt agcttctctt gtttcatcga gaatgataat gactagctct
    54901 tcaaaactga tgtctgggaa aaaacggctt tgccctaaat attctttcaa tgaatttcca
    54961 taatggtttc caaaaaaagt atccatgata aaatgccgtc gatttctttc ttccccttcg
    55021 atccatgtcc cttgaccatg cttagaaacg atttttagtg aatatggaat caatcgctcc
    55081 ttgatttctt gcatgtcctt tgtcaaactg gaacgactga tataaagctc ttctgccaaa
    55141 tcatccataa aaagaatggc atcttccaaa agcaattggt tcaatatata cttttgtcga
    55201 tcttcggact cataaaatcc ttgcggttct ctagcatatc caggtactat ttcttgccga
    55261 ctcaaaaaca agtcaaatgt cagtctttgt aaaatatgca gttggtagcc ataacctggt
    55321 ttagcaatga tcttacctcc gtttttttct actaactctt tgatccgctg caagtaatta
    55381 cggattgtcc gatcagataa taacaattca gaagctaaac gttgactggt gacatactcc
    55441 ttcttgtgat caatcaaata tctcaacagc aatttttcct tctgtttcat tatcatccac
    55501 tcctattcca tcatatgttc tcttcgatca cctctgctaa cttctggatg cctgtaggga
    55561 ttgggacata cgcttggaaa gggatactaa caacaggctt tccaacagat tctcctaatt
    55621 ttttgaattt atccaaaaac atcgtcgttt gcgggctgat cagaaataaa tcaaaatcac
    55681 tattcttaat catattatcg ccttctgtag ctgaaactgc atctacgagt acctcttctc
    55741 ttttttgctt aaagaaatcc gttgtctttg aggccatcat cgaggatgac attcctgccg
    55801 cacaaataat caatgctttt ttcataaatc aatcactcct tttattttta taaaccaagc
    55861 atatcttgta agcgttttct attcaaaata tatatttccg tagcattgcg gaaaaaatgt
    55921 gtatcatatc ttgaataata atttctggca ccgtgatata ctagtgatag aattttttca
    55981 ggaggggatt tagtgagtat cacagtgaat attttgtata caagtacaaa tggacaagcc
    56041 agaaaatttg cagaagaaat ggtcgaaaga aacattgtca aagcgattcg tgcagaagaa
    56101 ggaaacgaga aatatgacta ttactttcca ctagaagatc cagaatctct tttactgatc
    56161 gaccgttgga aagatgaaga agcaatcgaa aagcatcaca aatccgaaat gatggctcaa
    56221 ataaacgaac tccgtaaaaa gtataaattg aaaatgaaag tcgaacgttt cactgattta
    56281 ccgtaaaaag atacacatgg aatgatagca atttatcatc catgtgtatc ttcacttttt
    56341 ccttattttt tattcgatct catctaatga ttcaaaccgt gtttttctct tatttttcgg
    56401 ataagtattt cagtcacccc aatatacatg attcctaaac ataccccaag gatattctca
    56461 aaaatgcgga tcgttactgc tcctggaacc ccgaataaac tggcagtgat tgccaaagca
    56521 ccaaaagaat taaaaactgt tttatatcta taggtagaac aaacacctaa agctagacca
    56581 cctaatatac cgacccagtt aaaaggaata aattggctga taccaataaa taaaacacat
    56641 ccaatcaatg agccaatgat tcggtctact gctcgttctt tgaaaccaat cgacatcaat
    56701 ccatagctag atacaataga agaaaatgca aaccctgccc acatcaatcg ctgaaaaggg
    56761 acatactctc ctgcaacgat caaaagacta atgccaaatg catagatgag catccaaatt
    56821 ttttcctgcg aatacatgcc tttcaaaaaa atctctttaa acaagctttt gtctttgttc
    56881 ttttctcgat gttttctgta aaggagaact gaaaaccaac aaaaaaacaa aaataataag
    56941 ctacttgttt gtgtaaagaa attcttgttc aacgaatctt taggtaacga ataaacaata
    57001 aagagatatg aaaagccgta caaagaagca ttccccattt tgggatcact agcggttgcc
    57061 attaatatgc tgcttagtaa aataaaatga atcaacatgc ccataactga ccatttgatc
    57121 agctggacat acggtgctac taatagtgag aacatgacga ttgctaaacc tatgatcgaa
    57181 tgggaaatct tataaccaaa gtcaacaaaa cgaatactca tcaacataca gaataaaacc
    57241 acagcgtatg gtttatgctt cgattcaaat aatgttgtaa ttataatgat aaaacaaatg
    57301 gcaaacaata cgatgaataa tgatcgtaaa aacaatgaac aaatatacat gcacttttct
    57361 tttttagaag ctgcttctcg tattttttgt tttaaaataa acggatctag ttgtagagac
    57421 tgataaaaat ccacctgttg tcgtcctttc tttttacttt tattttttac agtcatctat
    57481 gacaggttgt aaatactatg gaacatcata tggcgttgtc attttcttgt caactatttt
    57541 tcctattcat gtttttataa aaaaacatat cgttttcttg tatgattaaa tcaccacaaa
    57601 ataaacaact gagagaacga tatgctttat gaatggttct atcaaaaaaa ttctgagatc
    57661 aatagacaaa aatttgatga ttacagaggt ctcttacgaa accttacaga agaaaaaaac
    57721 attgatcgtt gctgcagttc tcttccctgc tccatgtgtc tgtagaaatt gtggttctgc
    57781 tgtggtagat ggaaatagga aattaatggt ggtgaaaaac aagaagaagg aaaccattgt
    57841 ccactttgaa taatataatc atatgccaat ggttatacgc ctaaaaaagc agcgctatac
    57901 ctgtaaaaac tgtcgaagcc attggaacgc tcaaagttat tttatccgtc caaggcactc
    57961 gatttcaaat catgtcagac ataaaatcac ttctttactg actgaaaaag tgtcgttatt
    58021 ttttatttcg aaaagttgtt aggtatcttt gacaaccgtt attcgcacat tgaaagagtt
    58081 gagaagctat ttaccaaagt cttccaaaag aattctgtct tgagtgttga gggttgtgaa
    58141 tttcgttctc acgtttccgt tgaggataaa ctgagtttta tttccgcgga tggcgagaca
    58201 gaaaaattaa tcgagaaaat ttacctacac aaaaaattcc tcgattaaca aactatttcc
    58261 ttagctgcac taatcctaaa gaaatagaat tcttagttac agacatgaac gccacctact
    58321 ttcagctcac caaacatgtt ctaccaaatg cgaaattggt gattgatcga ttacttggtt
    58381 tttccacgcc tttcaaggag gcgtaacctt tattcacgag ttggttgaag ctttccgaga
    58441 caaaagaccc gacttgtttt tctccttatt gacaaagagc cattaaaact agagaaaaaa
    58501 ttacaactga tcgctgatta tttgtagaaa gaaaatcaaa gtattaccca ataaacttga
    58561 gtcattaggc aaaaaacact gcgaaactca agaatgattt tcgcagtgtt tttgtatatt
    58621 ttttattaac tagtattact acaaaggttt atttttcact tctcaacgca gcacaaaatt
    58681 gtctttactt atttactggt tgggttcaac cttctctact ccgatactaa tatttgtttg
    58741 tatacccctt ctaaaatcgg tagtttccat tcaattgatg ggtactagtt gattttaaaa
    58801 actatgctta ttcaattttt tattttagct catatatttc gtttctttct aaattttata
    58861 ctgatgaaaa caagtagcac acccaatatt gacaatataa actggctaat gatggtttct
    58921 ccagtttttg gaagatgctt atttccactg cttgttggat tgtttcctct tatatctgag
    58981 ctatcagcaa cttttctatt ctcttttgtt aaaaagatcg ccacttcttg ttctttttcg
    59041 atcttgaaag ttactggtgt cgcatccaat tcataccccg ttggcgcttg agtttctacc
    59101 agctgatatg ctcctggtgc taagccatct atcgccagtt ttccatctgc tcctgtggtt
    59161 agacctgact gcagaacggt tccattggca tcttgtaact caaaaacagc tccttgcagg
    59221 acttctccgc tttggtcatc gatctttgtc aacacgacac cgcctggtgt aagtcgattt
    59281 tctttcgtca attctaccac cgctgtctga cttctttcga tctcaaactc gattggtgtc
    59341 gcatccaatt cataccccgt tggcgcttgg gtttctacca gctgatacac tcctggtgct
    59401 aagccgtcaa tcgccagttt tccatctgct cctgtggtta gacctgactg cagaacggtt
    59461 ccattggcat cttgtaactc aaaaacagct ccttgcagga cttccccgct ttggtcatcg
    59521 atctttgtca acacgacacc gcctggtgta agtcggtttg ctttcgtcaa ttctaccacc
    59581 gctgtctgac ttctttcgat ctcaaactcg atcggtgtcg catctaattc ataccccgtt
    59641 ggcgcttgag tttctaccag ctgatatg
//
LOCUS       DAZABD010000010        53526 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000010 DAZABD010000000
VERSION     DAZABD010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 53526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.010"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(70..921)
                     /locus_tag="ACHALG_000676"
     CDS             complement(70..921)
                     /locus_tag="ACHALG_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFN2586111.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(934..1719)
                     /locus_tag="ACHALG_000677"
     CDS             complement(934..1719)
                     /locus_tag="ACHALG_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFN2586112.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            complement(2071..3012)
                     /gene="ribF"
                     /locus_tag="ACHALG_000678"
     CDS             complement(2071..3012)
                     /gene="ribF"
                     /locus_tag="ACHALG_000678"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HFN2586113.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            complement(3016..3939)
                     /gene="truB"
                     /locus_tag="ACHALG_000679"
     CDS             complement(3016..3939)
                     /gene="truB"
                     /locus_tag="ACHALG_000679"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFN2586114.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            complement(4307..6669)
                     /locus_tag="ACHALG_000680"
                     /pseudo
     CDS             complement(4307..6669)
                     /locus_tag="ACHALG_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
     gene            6834..7290
                     /locus_tag="ACHALG_000681"
                     /pseudo
     CDS             6834..7290
                     /locus_tag="ACHALG_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035543.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            complement(7447..7794)
                     /gene="rbfA"
                     /locus_tag="ACHALG_000682"
     CDS             complement(7447..7794)
                     /gene="rbfA"
                     /locus_tag="ACHALG_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFN2586115.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(7821..10127)
                     /gene="infB"
                     /locus_tag="ACHALG_000683"
     CDS             complement(7821..10127)
                     /gene="infB"
                     /locus_tag="ACHALG_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFN2586116.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNPNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            complement(10140..10451)
                     /locus_tag="ACHALG_000684"
     CDS             complement(10140..10451)
                     /locus_tag="ACHALG_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HFN2586117.1"
                     /translation="MNTVNRQEAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            complement(10448..10741)
                     /locus_tag="ACHALG_000685"
     CDS             complement(10448..10741)
                     /locus_tag="ACHALG_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HFN2586118.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            complement(10763..11938)
                     /gene="nusA"
                     /locus_tag="ACHALG_000686"
     CDS             complement(10763..11938)
                     /gene="nusA"
                     /locus_tag="ACHALG_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFN2586119.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            complement(11961..12434)
                     /gene="rimP"
                     /locus_tag="ACHALG_000687"
     CDS             complement(11961..12434)
                     /gene="rimP"
                     /locus_tag="ACHALG_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFN2586120.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            complement(12579..16937)
                     /locus_tag="ACHALG_000688"
     CDS             complement(12579..16937)
                     /locus_tag="ACHALG_000688"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFN2586121.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            complement(17149..18858)
                     /locus_tag="ACHALG_000689"
     CDS             complement(17149..18858)
                     /locus_tag="ACHALG_000689"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFN2586122.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELVNTLPILLN
                     QGE"
     gene            complement(18926..20194)
                     /gene="rseP"
                     /locus_tag="ACHALG_000690"
     CDS             complement(18926..20194)
                     /gene="rseP"
                     /locus_tag="ACHALG_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFN2586123.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAILLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPDKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            complement(20355..21155)
                     /locus_tag="ACHALG_000691"
     CDS             complement(20355..21155)
                     /locus_tag="ACHALG_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFN2586124.1"
                     /translation="MKQRVVTAAVALVLFIPIIWIGGIAVELTAAILAVIGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            complement(21152..21964)
                     /locus_tag="ACHALG_000692"
     CDS             complement(21152..21964)
                     /locus_tag="ACHALG_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFN2586125.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            complement(22159..22716)
                     /gene="frr"
                     /locus_tag="ACHALG_000693"
     CDS             complement(22159..22716)
                     /gene="frr"
                     /locus_tag="ACHALG_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFN2586126.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(22719..23441)
                     /gene="pyrH"
                     /locus_tag="ACHALG_000694"
     CDS             complement(22719..23441)
                     /gene="pyrH"
                     /locus_tag="ACHALG_000694"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFN2586127.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(23577..24458)
                     /gene="tsf"
                     /locus_tag="ACHALG_000695"
     CDS             complement(23577..24458)
                     /gene="tsf"
                     /locus_tag="ACHALG_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFN2586128.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(24557..25339)
                     /gene="rpsB"
                     /locus_tag="ACHALG_000696"
     CDS             complement(24557..25339)
                     /gene="rpsB"
                     /locus_tag="ACHALG_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFN2586129.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            25698..26177
                     /locus_tag="ACHALG_000697"
     CDS             25698..26177
                     /locus_tag="ACHALG_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFN2586130.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGYYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            26394..27485
                     /gene="phnW"
                     /locus_tag="ACHALG_000698"
     CDS             26394..27485
                     /gene="phnW"
                     /locus_tag="ACHALG_000698"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HFN2586131.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKELLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            27478..27606
                     /locus_tag="ACHALG_000699"
     CDS             27478..27606
                     /locus_tag="ACHALG_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HFN2586132.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            27610..28155
                     /locus_tag="ACHALG_000700"
     CDS             27610..28155
                     /locus_tag="ACHALG_000700"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HFN2586133.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(28609..30300)
                     /gene="argS"
                     /locus_tag="ACHALG_000701"
     CDS             complement(28609..30300)
                     /gene="argS"
                     /locus_tag="ACHALG_000701"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFN2586134.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(30720..31667)
                     /gene="arcC"
                     /locus_tag="ACHALG_000702"
     CDS             complement(30720..31667)
                     /gene="arcC"
                     /locus_tag="ACHALG_000702"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFN2586135.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(31782..32801)
                     /gene="argF"
                     /locus_tag="ACHALG_000703"
     CDS             complement(31782..32801)
                     /gene="argF"
                     /locus_tag="ACHALG_000703"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFN2586136.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(32892..34121)
                     /gene="arcA"
                     /locus_tag="ACHALG_000704"
     CDS             complement(32892..34121)
                     /gene="arcA"
                     /locus_tag="ACHALG_000704"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HFN2586137.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
     gene            34582..35283
                     /locus_tag="ACHALG_000705"
     CDS             34582..35283
                     /locus_tag="ACHALG_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFN2586138.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(35892..36908)
                     /gene="tsaD"
                     /locus_tag="ACHALG_000706"
     CDS             complement(35892..36908)
                     /gene="tsaD"
                     /locus_tag="ACHALG_000706"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFN2586139.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALAAKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(36905..37369)
                     /gene="rimI"
                     /locus_tag="ACHALG_000707"
     CDS             complement(36905..37369)
                     /gene="rimI"
                     /locus_tag="ACHALG_000707"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFN2586140.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(37376..37918)
                     /gene="rimI"
                     /locus_tag="ACHALG_000708"
     CDS             complement(37376..37918)
                     /gene="rimI"
                     /locus_tag="ACHALG_000708"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFN2586141.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(37902..38624)
                     /gene="tsaB"
                     /locus_tag="ACHALG_000709"
     CDS             complement(37902..38624)
                     /gene="tsaB"
                     /locus_tag="ACHALG_000709"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFN2586142.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(38815..39795)
                     /locus_tag="ACHALG_000710"
     CDS             complement(38815..39795)
                     /locus_tag="ACHALG_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN2586143.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(39819..41303)
                     /gene="galT"
                     /locus_tag="ACHALG_000711"
     CDS             complement(39819..41303)
                     /gene="galT"
                     /locus_tag="ACHALG_000711"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HFN2586144.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(41315..42304)
                     /gene="galE"
                     /locus_tag="ACHALG_000712"
     CDS             complement(41315..42304)
                     /gene="galE"
                     /locus_tag="ACHALG_000712"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFN2586145.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            42552..42719
                     /locus_tag="ACHALG_000713"
     CDS             42552..42719
                     /locus_tag="ACHALG_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HFN2586146.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(42781..44592)
                     /locus_tag="ACHALG_000714"
     CDS             complement(42781..44592)
                     /locus_tag="ACHALG_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HFN2586147.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(44589..44954)
                     /locus_tag="ACHALG_000715"
     CDS             complement(44589..44954)
                     /locus_tag="ACHALG_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFN2586148.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(45117..45512)
                     /locus_tag="ACHALG_000716"
     CDS             complement(45117..45512)
                     /locus_tag="ACHALG_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFN2586149.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(45530..46492)
                     /locus_tag="ACHALG_000717"
     CDS             complement(45530..46492)
                     /locus_tag="ACHALG_000717"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HFN2586150.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(46492..46704)
                     /locus_tag="ACHALG_000718"
     CDS             complement(46492..46704)
                     /locus_tag="ACHALG_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HFN2586151.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(46725..47423)
                     /locus_tag="ACHALG_000719"
     CDS             complement(46725..47423)
                     /locus_tag="ACHALG_000719"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFN2586152.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(47443..47985)
                     /locus_tag="ACHALG_000720"
     CDS             complement(47443..47985)
                     /locus_tag="ACHALG_000720"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFN2586153.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(48117..49121)
                     /locus_tag="ACHALG_000721"
     CDS             complement(48117..49121)
                     /locus_tag="ACHALG_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFN2586154.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(49118..50107)
                     /locus_tag="ACHALG_000722"
     CDS             complement(49118..50107)
                     /locus_tag="ACHALG_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFN2586155.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVEKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(50104..50910)
                     /locus_tag="ACHALG_000723"
     CDS             complement(50104..50910)
                     /locus_tag="ACHALG_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN2586156.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            51076..52032
                     /locus_tag="ACHALG_000724"
     CDS             51076..52032
                     /locus_tag="ACHALG_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN2586157.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(52109..52627)
                     /locus_tag="ACHALG_000725"
     CDS             complement(52109..52627)
                     /locus_tag="ACHALG_000725"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFN2586158.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(52715..52864)
                     /gene="rpmG"
                     /locus_tag="ACHALG_000726"
     CDS             complement(52715..52864)
                     /gene="rpmG"
                     /locus_tag="ACHALG_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFN2586159.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            53092..>53526
                     /locus_tag="ACHALG_000727"
     CDS             53092..>53526
                     /locus_tag="ACHALG_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFN2586160.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKE"
BASE COUNT        15014 a        11489 c         9376 g        17647 t
ORIGIN      
        1 tttataagta cttactgttt ggttcaaaca aatatttttt taataaatga aattttacca
       61 tctttatcct caaataaaga ccgtgaccat gcttcctact acgatcagca ataatccgat
      121 aatcgttaat tttttttctt ttggtgtctt attttcatgc aggatgtaca taccgcctaa
      181 tgtagagata actacgttta gctgtgtaat gatatatgca gtagcaaccc cattttcatt
      241 tgctgaaaaa atataagcca atgctgacac actaaagatg gctccaacga tcgttgcttt
      301 ccaactcttg tcttcagaaa atgcggttgg tttacggaaa atcacataaa tcactgcccc
      361 taaaaagaca ccgatcattt gcggaaagaa aatggaaagt ccgcttgcat caactagttt
      421 aggcaatgca ctgtataccc aataaccaat acttgttgat gctaatagac taattccttt
      481 tgtgataccg cctttttttc caggatcatc actgattgcc gttaaagcag aaccaaatac
      541 aagaagaatg attgctgctg ctccaatgat tttccccata cttccagacc attctccaaa
      601 agcgaagaca ccaatcaacg aggttccgac taactgtaat gcagtcgaaa ttggcatcgt
      661 tttagaaaca ccaatcaagt tgaaagatgt atactgtcct gtttgtccaa taacccaaaa
      721 tgcaccagaa agtaaactaa tcaaaaatgt agtcactgaa ataccagctg gagaagcgaa
      781 taattgaacg agtaagccga caagcaccgc tccaatacca gttcctaata tctcatttgt
      841 tggtctgcca ccaatctttt tcaaaatcag tggctggatc ccccagccaa ctgccgggat
      901 caatgcaatc aataaaccca tttttattca cccttatcct tttccacctt ggaaagaagg
      961 atataaagtc atcccaccat ctgcaaatag tgtaatacct gtaacataac tggattcgtc
     1021 tgaagccaac catgcagctg tcgccgctat ttcttttggt tcacctacgc gtcctagtgg
     1081 gatcatactt tccaagagtt ctttttgttc aggatcatca aattttgccg cattaatagg
     1141 tgtacggata gcaccagggc ctatactatt gattctgatt cccttcggtg catactccat
     1201 tgctaccgtt tcggtaaata attttacccc gccttttgaa gcagcgtaat gagcaaacgt
     1261 tggccatggg atctgttcat gaacagaaga catattgatg atatttcctt ttttattatt
     1321 ctctgtaaag taatttaaag ccgctttcgt tccgataaaa acgccagtca aattgacttt
     1381 taacactctt tcccaatctt caaggctaag ttcatgggtt ggatgctgat tttccatccc
     1441 agcattatta acccaaacgt ccagtgtccc aaaattttct aaagccgtat caagtaaacg
     1501 ctgtattcct tcttcagaac tgacatctgc ttgtacagag actgcttttc cgcctgcctc
     1561 ttcgattgct ttgactgctt tttctgctcc ttctttatcc gagttatagt tgacaactac
     1621 attcattttt tctttcccga atcgttgagc aatcgctgtt ccaatcccct ttgagccacc
     1681 agttactact gcgactttgt tttctaattc cttgtacata gtacccactc ctttttcata
     1741 cctaaacctg ttattacgga taacttttat ttccgcctta aagtgagtgt acaccaagat
     1801 atatagtcta aagcaaatga tacgctctag caatcgtttt catgaattgc tagtgtcctt
     1861 ttatttctat aaagaaagag gttgtgacag aagtggtcag cttcaagaaa tttcggctta
     1921 tttccgaaga agctacttct gaaaaaccat ttatttggaa aaaaaggctg tgacaagact
     1981 tttgtcacag cccctttcct gggcttattt atcacccatt ttattgtaat tgctagttat
     2041 acataaacaa gagattttat gcataatgtt ttattgctga gaaaaatatt cagctgttgc
     2101 ttgttcatcc tccttgagtt gttcaatcaa ttcctctaca ctagaaaatt tcacttgatc
     2161 tcttaaaaat tgattccagc gtaccgcgac atgttcacca tagatatctt gattaaagtc
     2221 caaaatatac aactctacag tcagctcccg tccttcaccg aatgtatcat tatgtccaat
     2281 tgaacccatg gctggatacc atgtttcccc aatttttatt tccgacacat aaacaccttc
     2341 tttaggcaaa tgaaccgtgc tctttacttt gacatttgct gtagggaatc ctagtagtct
     2401 gcctcgtgca tcgccatgga caactacccc ttctacctca tatgtgcaac ctaatagttt
     2461 tgctgcttct tttacttgtc cgctgtctag taatccacga atacgggtag agctgatttt
     2521 ttcaccttgt tcttcttctt tagaaactgt gacgattttg aatcttcctt tggcatattc
     2581 aggaaaatga gcgacatctg caatgtcttt tggtccatac gtataatcaa aaccggaaac
     2641 agcatatttt gcatgtaacc ctacgatgta ttgatcaaca aattcctgcg gtgctaaatg
     2701 agcaaaagaa gaagtaaaat caacttcata taaataatct atacccaaag cggccatttt
     2761 ttcttctttt tgcttaaggc tagtcaaata tttcacgctg ctaggatcga tctttttaaa
     2821 gacaatagat ggatgctgat tgaaagtcat caatgctaat ttcaatcctt ctttttgtgc
     2881 aatttccata cctgtcttta tgactttctg gtgtccgata tgcacgccgt cgaaaaatcc
     2941 cagcactaaa acgacatcgt cttctggaat ttgttctgat tggtaaggat gtctgatctt
     3001 gatgatattc attcgttatt cctcatttct caatacttta ctaggtttta atttgttttt
     3061 ttctttcggg tttggctgat aaatactgac aacttttcct ttataaaaca aagcaatttc
     3121 ttctgacggc atttctggga gcgcaaatac agtataatca agtctcatac cgttttttac
     3181 tttctgccat acttcatcag taatatctac tcgtttgaac cgttccactc ctttttctat
     3241 tggcagcaaa caatcatcga ttgtcccatt tttcacagct tgagctactt cttctaatgt
     3301 cagagcatcc ttctgggaca taccggcact tgctgttcgg gtcagatcag acatgtgcgc
     3361 agggtaacca agtttcttgc ctgtatctac agctaatgtg cggacatagg ttcctttccc
     3421 gcattctact tcgaagcgcc aagaaatggt tcctgtcact tcgtctcttg tcaattcact
     3481 tgtccggata aaacggtaaa tctgagcctt tctaacagga cgctccactg tttcattatt
     3541 gcgggcatat tcatataacc gcttaccgtt gacttttacc gctgagtaca ttggtggtat
     3601 ctgggtgatc tcaccaataa atgaagccat cacttcatct attttttttt cagataaaga
     3661 catatctact ggttttgttt ctacccgttc tccggatcga tcttccgtcg ttgtactgaa
     3721 acctagagtt atctcgccta catagatttt cccagaatct gtcaaatatt cgataacttt
     3781 tgtggctttt ccaatacaaa tgggcaacac accgtctacg tctgggtcaa gcgtaccccc
     3841 atgtccgatt tttttcgtat gtaagatttt tcttaatttg aatacgcaat catgacttgt
     3901 cattccgcgt tctttccata aaggtaataa cccgtccatg tcttttctcc atttcatttt
     3961 tgatttaact gaattattat aacacattgc atcaattact gacgaatttg acatcataat
     4021 gaacggtttt tatttttcgg caataaaaac gtaacaaaat tcatgctcac gtaaatagaa
     4081 atcatgttat gcttttttaa aggagggaat agaatggaag attttttctt tgcaccattt
     4141 gattggattt atgggtggct tacaggtaaa tagtttggtt ttcacttccc aaaaacgaaa
     4201 aaaaagactg gccattcttc cagtcttttt tccgtttttt ttatttattt tttcagtttc
     4261 ttaatcaatg taaaaaattc tattttgcgg gatcgatttt atcattttat tggtcaagat
     4321 ttttccattt ccaatccaat acttctggca gatcaatacc attttcacga atatagtttt
     4381 gatgttcttg taatttctga tccatttttt tcgcaaaatc ttctgcttga cttcctttga
     4441 tttttaaagc tgcgtctttt gccaaatgat aacgatcaag ttcatttact acacgcatgt
     4501 caaatggtgt cgtgatatct ccattttctt tgtagccatg aatcattaaa tgatggttat
     4561 gacgatcaaa gaaaatcgtg cggaccaatg tttcataacc gtggaaagca aataagatcg
     4621 gtttgtcttt tgtaaaatat tgatcaaatt cttcatcgct caacccacgc ggatcttgac
     4681 ttggatgacg taatttcaat aaatctacga cattgatgaa ccgaattttt agttttggga
     4741 attcttccag cagcaaagtt acagctgcta atgcttctaa gttagattcc gttcctgcag
     4801 cagcaatgac caattcaggt tcctcctctt ttgctgtcga agcccaatca atgattttca
     4861 agccttcatt cactaagacg gctgcttctt ctgctgagta aaattgagca cgcggatgtt
     4921 tggaagagat gatcaaattg attttttctg acgagcggaa tgctttatcc atgactgcga
     4981 gcaatgtatt tgcatccgca ggcagatatt cacggatgaa ctccgccttc ttttctgcca
     5041 aatgtgtcaa tattcctgga tcttggtggc tatatccatt gtgatcttgc tggaagactg
     5101 ttgagcttgc aatgagattt agcgaaggat aatcatttcg ccaagaaagg tcatgtgatt
     5161 tccgcatcca tttgaagtgc tgagtcaaca tagagtcaac cactcgcaag aatgattcgt
     5221 aacttgcaaa aaaccatgcc gtccagtcag aacgtaccct tctaagaacc cttcatcttg
     5281 gtgttccgat aattgactgt cgatgacgcg accagatggc gaaagccatt catcattttc
     5341 tggttcgatt ttttccatcc attggcgatt tgtcgttttg aaaacttgat tcaatcggtt
     5401 tgattttgtt tcatctggtc caaatatacg gaaattttct ggatttttct caatgatctc
     5461 gcgaacgaat ttaccaagtt cggtcatatc ttctgcttta acggagcctg gtttttcaaa
     5521 ttgcaaagta tattttttcc aatctggcat aataagcggt cttggatcga ttccaccatt
     5581 ggtgatagga ttttttgcca ttctttggtc accagtaggc gcgatttctc tgatttcttc
     5641 taaaatacgt ccttgtgcat caaataattt ttctggttca taggatttca gccaacggag
     5701 taaagcatct gcatgatcca tatgttcttg atctacagga atcggtactt ggtgtgctcg
     5761 gaaggttcct tcaattggtt ccccgtccca ttcttttgga ccggtccatc cttttggtgt
     5821 gcgtacgatc aatactggcc attttggcat cacgacatct gttgctgtct tcaggcgagc
     5881 ttctttttgg atggatttaa ttttctcgat tgcttgatcc attttttcag ccataacagg
     5941 atttaatttt tctgaatcat ttccttcaat aaagaaaggt tcccagccaa gtccgttaaa
     6001 gtaatttgct aattctccat ctgatttacg tgccaagatc gttgggttgg ctatttttgc
     6061 accatttaaa tgaaggatcg gtaaaacagc tccatctatc actggattga taaagacatt
     6121 tgagaaccag cttccagcta aaggaccggt ttccgcttca ccatcaccaa tcacgacagc
     6181 agagattact tcaggattgt ctaatacagc tccgactgca tgtgaaagag agtatccaag
     6241 ctctccgcct tcatggatag aacctggtgt ttctggtgct gcatgagaag caatgcctcc
     6301 aggaaatgaa aatcgtttga ataatttttg cattcctgtc tcatcttccg ttacttctgg
     6361 atagatctct gtgtaacttc catctaaata agcattcgat accatgactt gtccgccgtg
     6421 tcctggccct tcaatataaa acatgttcaa atcatattta ttgatgactc ggttcaaatg
     6481 gacataaata aaattttggc caggaatcgt tccccaatga ccgattggat gttttttgac
     6541 atcttcgggt ttcagtgtac gtctcagtaa ggggttatct ttaagataaa gttgcccaac
     6601 agaaagataa ttagcagcac gccaccaagc cgtcatttta tcaaagtatt ctttactaga
     6661 ataatccata cataacactc cttttcatta ttactcttat tctagcgcag atactcccaa
     6721 ttgaaaccta ataagcactt ctgttcattt gtttggattt tatatctagc caaatgaaca
     6781 ggtataaaaa accacgaaat ttttcctttc gctgacgttt tttacattga tccaaaaaac
     6841 ttgtcgttat cacaatggct gttccgcagt tcatttcgct cctcatcatg cataatttgc
     6901 tgaatgacgc tgggccagtt aacacattgc tggaaaactg gggctggatc gagcaattcc
     6961 tttcctgatt gatccgtttt atgtaaaagt ctctgttatc tttgtcaata tgtgaattgg
     7021 tatcccagct actatgctaa tttctacagg gattatccaa aatttggatc gtgaccaaat
     7081 cgaagcggcg taatcggaaa tatcaataac ttcaacgtga tttttctttt aactgtcaac
     7141 ggaccgacaa actcaaactt ttatggtgca ggttccactg atcttctagt tacttggcta
     7201 tacctgactg tcactaaaat agattacaat ctagcttccg ttatcggtat cttgatcttt
     7261 atctatatgc aaaaatttat atactactag aaaaaagaaa gtcgaatttt ttacttttct
     7321 ttttctcttt tttggttagc tgacacgccg caagactaaa gtcatggaac tcttaggtaa
     7381 tacgtcttaa tgaaaacaaa aaaatcccgc aacccctgat ttcacaggat ttgcgggagt
     7441 ttttgattag tctttgttca agtcgcgaag caattcatcg atatggttgc catagacaac
     7501 tgattcatca agttcaaaga acaattcagg tgtcttataa atacttagat tgtgccctaa
     7561 ctctcgacga atcaatcctg atgctttatt caatccatct tgtgctgttt tcttatctga
     7621 tgctaagtca gataaaatgc tgtaatagat cgttgcttgc tgtaagtccc ccgtaacttt
     7681 cacatctgtg atcgttacgt tctccacacg agggtcacgg acctttttgc gcaaaatatc
     7741 attgacttct tttaagattt cttgaccgac tcggcggtca cggtagttag ccataatgat
     7801 tggcctcctt ttatcagttt ttagtcattt ttgacttctt ccatgataaa tccttcaatt
     7861 acgtcatcta ctttgatgtc attgaatttt tcaatcattg caccacattc ataacccatt
     7921 ttgacttctt tcacgtcatc tttgaatcgt ttcagacttg caagttggcc ttcatagatt
     7981 acgattccgt cacggattac acggacaccg ctatctctac gaatatatcc ttctgttaca
     8041 taggcacctg caatcgtacc aactttagaa actttgaagg tttcacgaac gatcatttga
     8101 ccggtgattt tttcttcaaa ttcagggtct agcatccctt tcatcgcagt ttcgatttct
     8161 tcgatggctt tatagatgat acggtgaaga cgaatatcga cttcttcagt atcagcttgc
     8221 acttttgcct gcggtgtagg acgaacgttg aaaccgatga taatagcgtt gctggctgct
     8281 gctaaggtta catcactttc attgattgct ccgacagctg aatggacgat ttttacacgg
     8341 acaccttcca catcaatctt cttcaatgat gcagccagtg cttctgctga accttgtaca
     8401 tctgctttga tgattacgtt aacttctttc aattcgcctt ctttcaggct ttcaaataag
     8461 ttatccaaag tcacacggct tgtggcagca cggttttcta ataatgcacg tttcgcgcgt
     8521 tcttcacccg ctgcacgtgc tgtcttttcg tcttcgaaga ctacgaaacg gtcacctgct
     8581 tgaggtacat cattcaagcc agtgatttct actggcgttg caggtcctgc ttctttatcg
     8641 cgacggccaa gatcgtttgt cattacacgg acacgtccgt aagtattacc gacaacgatt
     8701 gggtcaccaa catgaagtgt tccttcttgg acaagtaagg ttgacacagg acctttacct
     8761 ttatcaagac gtgcttcgat cactgttccg attgcgcgtt gtgttggatc tgcttttaaa
     8821 tcttctactt ctgcgactaa caggatcatt tccagcaatt cttcgatgtt ttggccgaat
     8881 ttcgctgaga tctcaacaaa gatcgtttct ccaccccatg cttcaggaat taactcatac
     8941 tcacttaatt cttgcataac atgttgaggg tttgcacctg gtttatcaat tttatttaca
     9001 gcgacgatga ttggaacgcc tgcagctttg gcatggttga ttgcttcaac tgtttgtggc
     9061 atgacgccat catctgcagc aacaactaag atcgtgatat ctgtgatact tgctccacgt
     9121 gcacgcatac ttgtaaaggc cgcatgtcct ggtgtatcca agaaagtgat tggtttacca
     9181 tcaatatcga tctggtaggc accgatatgc tgtgtgattc cgccggcttc gccgcttgtt
     9241 acacgtgaat ggcgtaatgt atccaataaa gttgttttcc catggtctac gtgtcccata
     9301 atcgttacga ctggcggacg tgagactaat ttatctggat cgacttcttc tgcctcgaag
     9361 aatttatcga tatcagcaat gtcaacttgg actttttctt gtggttccat gccatagtcc
     9421 gtcgctaaca gttcaatcgt atctttatcc agtgcttggt tttgattgac catgacacca
     9481 agcataaata attttttaat gatttctgct ggctcacgat gaattttttt cgcaatatct
     9541 gctacgttca taccttcagt atactctaat acatctggca attcacggaa tttgcgtggt
     9601 ggtacagctg gtttagttga ttgttgctgt ttgccttttt tacctttttt attataacga
     9661 ttacggttat tattattaaa tttgccgcga ttgttattgt ttggtttgtt gttattgtta
     9721 gtacggtttt gattgccgcc ttggctcgtt ccttgacgat tttggctgct ggtatttccc
     9781 gtattatttg ggtttgttgt tggttttttt ccttgattca cttgaccgct cccttgaccg
     9841 cgatcttgat aactgcggtt atttttattt ttttgttgat tgttcgttgt ttctttttta
     9901 cgttgatcgt tcggctgttg attggctggt tttctgcttg cttgttgtgc agcttgttgt
     9961 ttcgtgcttg ggtttttgaa cgcttgttgc aatttcttct catcattgct agtaatcgca
    10021 cccatatggt ttttcacatc gatgccaagt tgttgtgctt tttccacgac gtctttacta
    10081 gattttttga gttcttttgc taattcatag attcttttgt tgcccatgca atcaccttcc
    10141 taaccttcaa ttagctcctt ggtcttcttc gcaaatccgg catccattat tccgaatacc
    10201 ttgcgaggtt ttccgatcat ctgcgtgatt tctgcttcag aaaatagctc aaagcaagga
    10261 acttcataat atgaactctt atcttttatc ttttttcttg tattctcgct tgcatcggtt
    10321 gccacaaaca ccaattttgc tttttgtttg cggatttcag caatggtcag ttcttcaccc
    10381 gtgattagtt ttcccgcgcg catcgctaga ccgatgagat tcatggcttc ttgccgatta
    10441 actgtattca ttttccaaat aactctttac gtgctttttg gtgagtgaca tagtcaagaa
    10501 gttcttgata aaactcatct gttaatgttg tttcgagtac tcgatcgaga atttttttat
    10561 cccaagcttg ttgtacttct tccggctcaa gtgagacata cgctcctcgt ccaggcattt
    10621 ttccagttgg atcgatcgat acttcccctt cttttgaacg ggtgattcgg accatctctt
    10681 ttttaggttt catttcacct gaaaccacgg atttgcgtaa aggaattttc ctttgtttca
    10741 tctctttgcc tccttactaa agttaagctt ctggtttttc ttcgaccgtt tcattgttga
    10801 atgcaattgt ttcatattca tcgtctgtta aatctgactg aacgattgct tcatcatgca
    10861 gttcttctgt ttctttttgt tgttcttgat tttcataaaa ttccgtcatg tcagattctg
    10921 atttgatatc gattttatgg ttagtcaatt tagcagctaa acgtgcgttt tgtccgcgtt
    10981 taccgattgc tagcgataat tggtagtctg gaacgacaac tgtgcaggcc tttggatttg
    11041 tctcatcgaa gatcacatcc atgacttgtg caggattcaa tgcatttgca atgaatacag
    11101 ctggatcttc attccattcg acgatatcca tgttttcgcc ttttaattca ttgacgattg
    11161 cttgaactcg ttgtcccttt ggcccgacac atgtaccgac tgcatcgata tttggatcat
    11221 ttgagcgaac tgccactttt gaacgatcgc cagcttcacg ggcgatgctg acaatttcaa
    11281 cgattccatc atagacttct ggtacttctt gctcaaataa gcggcgcaat aggtctggat
    11341 ggctgcggct aacaaatact tgcggacctt tagacgtatt ttctactcgg gaaacataaa
    11401 ctttgattcg atcatgtggt tgataaaatt catttggcat ttgatcttgt ttagaaagaa
    11461 ctgcctcaat tttaccaaga ttgacataaa tataacgttt atcctggcgt tcgacgattc
    11521 cttgcatgat gtctttttca taagcactga attcgttata aataatcgtt ctttctgctt
    11581 cacgtacacg ctgtaaaata acttgtttag ccgtttgcgc agcgatccgc ccaaaatctt
    11641 ttggtgttac ttcaaaacgg atcgtatcac cgatctcata tgctggattg attaaaagtg
    11701 catctttcaa tgagacttcc aactgtgaat ccatcacttc ttccgttact tcttttaccg
    11761 catagacatg gatctttcct tttttctgat caaattccac ttcgacgttt tgtgcttggc
    11821 catagtgacg tttgtatgca gaaaccaatg ctgcttctaa agcatcgatc acgatttctt
    11881 ttgagatccc tttttcagct tctaaagcgt ctaacgcatt taacatttct ttactcattt
    11941 tttctgattc ctctccgtga ttaaaattgg atagctaagc gagcttttgc aatatttttt
    12001 cggtcaaaag tcaattcttt ttctcgtgtt tttatgcgta ttttcaatgt aagctgttca
    12061 gcatcaaatg attgaagaaa tccttcatat tgtttttcgc catctactgg ttggtataag
    12121 gagacatgga cataatcacc aagtgccttt tcatagtcgc tttctttttt caatggccgc
    12181 tctgctcccg gggaggatac ctccaggaaa taggcttgag gaatcggatc tggatctgct
    12241 gcatcaagtt tttcactcag tttctcactg acaaaggcgc attcttcgat atcgataccg
    12301 ccttctttgt caataaatac tcgtaagaac cagctttttc cttcttttac aaattcgact
    12361 tcgacaagtt caaaattttg ttcatctaaa atcggtgtca ccatttctgt gactgtttca
    12421 acgacgctac tcaaaagttt cgcctcctta aaaaattaag tgcgtaattc cattaaaaag
    12481 agtgagcgaa aaatccgctc actcacagtt agtatcatta cctataagaa ctataccata
    12541 ctctgcttaa aattacaagc agacaggaaa acagatgctt atagcatatc gaataacgat
    12601 aattgatttt catctggtag atctttcaat acgccattgt cgttcatata ttcgatcaat
    12661 gtttttgaaa ctttaccgcg ggtggcaaga tcttctttgg ataaaaatgg accgtctttt
    12721 cttgcttcaa cgatttgttt tgccacgttg gttcccaaac ttgggacagc tctaaatggt
    12781 gcgatcaacg tatcaccttc aataacaaag ttaacggcat ccgatttata caaatcaatc
    12841 attccaaatt tgaatcctcg ttccagcatt tcattggcta actccagcac tgtcagttga
    12901 ttcttttctt ttacggaagc atctaatcct ttgtccgtga tttctttcat tgcttgtttc
    12961 acagcatctt ttcctttgca catggagact agatcaaagt cgtctgcacg tacagaaaag
    13021 tatgcacagt aatacaaaat agggaaataa accttgaaat aagcgacacg aagagccatc
    13081 aatacgtaag ctgcagcatg ggctttcgga aacatatatt tgatttttga acaagaatcg
    13141 atataccagt cgggcacatt attttctttc atcgccgata gataggtttc tcgcaattca
    13201 tcagggatct tgttccattg tcctttacga acagtttcca tgattttaaa tgccatcccg
    13261 ctatctaatc cagcatggat caaataaacc ataatatcat cacgacagcc gattacttcc
    13321 gccagtgtcg catctcctcg tcgaatcaat tcttccgcat ttcctaacca tacgtctgta
    13381 ccatgagata gtccggaaat ttgcagtagt tctgcaaaag ttgtaggatg cgtttcttct
    13441 agcatccctc gaacaaaacg agtgccgaat tctggaatac ctaatgtccc tgttttggaa
    13501 taaatttgtt cctgacttac tccaagtact tcaggaccgg caaatatccg catcacttcc
    13561 gggtcatccg tagggatcgt ttgtggatcg atccctgata aatcttgaag catccgaata
    13621 acagttgggt catcatgccc tagaatatct aatttcaata cattatcgtg aatcgagtga
    13681 aaatcaaaat gagtcgtttt ccattccgaa ttttgatcgt ctgctggata ttggatcggc
    13741 gtaaaatcat agacatccat ataatcggga ataacgatga tcccccctgg atgctggccg
    13801 gttgtccgtt tgactccagt agcgcctttt gctaatctgt cgacttctgc acttcgaaac
    13861 tgcagattat tgtccctttc gtatcctttt acatagccat atgctgtttt gtcggcgact
    13921 gtaccaatcg ttccggcacg gtaaacatag tcttcgccaa acaatacttt cgtatagtta
    13981 tgggcttctg cttgataatc acctgagaag ttcaaatcaa tatcgggcac tttgtctcca
    14041 tggaatccta aaaatgtctc aaatggaata tcatgaccgt ctttgtttaa gcgtgcacca
    14101 cattttggac attgtttttc tggcatgtca aatccagaac catacgtacc atcttcaaaa
    14161 aactcggaat attggcactc tggacaataa taatgagggg ctaacggatt aacttcagtg
    14221 attcctgtca tcgtcgcaac aaagctagag ccaacagagc cacgtgaacc tactagatag
    14281 ccatcttcat tacttttatg cactagtttt tgggcgatca gataaataac cgagaagcca
    14341 ttaccattaa tagaattcaa ctcttttttt aatcgttttt gaatgatctc aggtaacgga
    14401 tctccgtaca tttgttttgc ttttgtgtaa ctcaattctg atatttcatc ttctgaacct
    14461 ggaatcttcg gtgtataaag ttcatctttg actggaatca cttcttcgca gatatctgcg
    14521 attttattcg tattttctac tacgatctct ttcgctgttt cttcccctaa aaagtgaaat
    14581 gcagttagca tctcatcggt tgttcggaaa tggacatccg gcaaactatg acgattcaac
    14641 ggattcgctc cgcccataga attgatcaaa atttttcggt aaatggcatc ttcttcgttc
    14701 agataatgta cattacctgt tgcaacaacg attttatcca aactttttcc gatttctacc
    14761 aaattttgga taatttcttc caaatcatgt tcatttttta ccaattcttg ctcgatcaaa
    14821 ggcgcataga ccgcttttgg cataacttca atataatcgt agaattttgc tcggtttctt
    14881 gcctcttcta cccctttttg catcatggct tcgaatattt ctcccttatc acaagctgag
    14941 ccgatcaata gattttcacg catctttttc aattgggagc gtggtattcg tgggacgcgt
    15001 tcgaagtatt cgacattcga cattgaaatc agtttgaaca aatctttcag tcctgcctga
    15061 ttttttgcta aaatcgttac atggaaaggt cgagcccttt tgtaagaatc tccttctccg
    15121 atatgctcat ttagctgatc atgataaagc atgttatgat tgtccattgc ctctttcaca
    15181 aagatccatg ctaagtgacc agttgcttca gcatcataga ttgctcggtg atgctgttcc
    15241 aagctgacac caaatttttt agataacaca cctaatccga aacgtttgaa ttgaggatac
    15301 agatatctag ctaattccaa cgtatcaatg actggattgg ccgcttcagg aataccatac
    15361 cttgcgtagc tagtattcaa gaaccccata tcaaaagccg cgttgtgggc taccaaaatt
    15421 gtatcttttg aaaattcaag gaacatccgc aatacttctt cttccgactt tgatcctcga
    15481 accattccat ccgtgatacc agtcagatct accgttgttc gtgacaaagg atgtcctgga
    15541 tcgataaatt catcaaagct ttcgatcaca tttcctttat acattttgac tgcggctagc
    15601 tcgatgatcg tgtcatatac tgcagacaat cctgtcgttt ccacgtcaaa tacgacatat
    15661 gtggcttcac tcaatgcttc atgtgcatca ttataagcaa ttgggacacc atcatctacg
    15721 acattcgctt ccacgccgta taagattttc acacctgctt tttttccagc actatgtgct
    15781 tccggaaagg cttgcgcacc accgtggtct gtaatggcga tagctcggtg tccccatttc
    15841 cctgcttgtg ccacaagatc agagatgctg tttgttgcat ccatcgtact catattgcta
    15901 tgaacatgaa gctctactcg tttttcccct tcaggagcat aatctttgcg aggtgtatgt
    15961 ttgacttcta tgatatcttg cgcattcatg accaaatcac ggacaaacgt atcttcttga
    16021 atacttcctc gtacctttaa ccagctaccg acactgatcg catcaaatac ttgttcatct
    16081 ttttcgccgt tcgagaattt tttgacgata aatgatgaag tataatctgt gattttcaaa
    16141 gtcaagatct tgcgttttga acgtagttct ctgacttctt tgtcgaatac gtacccctca
    16201 agtgtgactc ttcgttcttc ttcaacgata ctgatcattg gtgtcgttgg ttcatcggct
    16261 ggaatatttc gtcctagctg gatgtggatg ggtccttcaa gagctggcga ttgctctttt
    16321 ctttcctttt tcttttgttc gtggacaact agactttcag ctgcttgctt cataaaggct
    16381 tctgcctgtt cttgtttgcg ttcttcaaaa agtttcaata ctcgttccgc ttgctgctca
    16441 tccacttctg gttcgatacg aaattttggg aatccgtaac tgacgaacaa ctcttctact
    16501 aatggcaagt attgttgctt caaataagaa atagctcctg tactgtcgat tggcaagatg
    16561 actttacgat cttttatcac aggtacctgc gttttcaata cttgctgtgc tagtggtgta
    16621 tcgcattgct gattttccaa agcttgggcc cagtaatctt gcaagagctg ttcatcgaat
    16681 gtctggtcat ctgctaagat attcagtttg acttgtgcaa tatctttaaa agcgatagtc
    16741 agatgctgag tcaatgaccg atatagcata ataggcaaaa ttttggaaaa atgcagcgta
    16801 aattcccata aacgcgattt acgatggacc actacactat ccatttcacc agcagtaagc
    16861 agtggatgat ttttttctgc ttcctctagt tggatttgtt ccaacagttt ttcaaataaa
    16921 tctcgttttt ctgacaaaga tatacgccct ttctgatgag aaacaatgca attgatcgca
    16981 tcttatcctc ttctttttca gtacctttta gtatactcgt tttttctgag ttttacatta
    17041 aaaaattgga actgcgattc aaatcgcagt tccaatttta aaagcgaatc atcagattgt
    17101 caaaaacagc tgattcagct atttttattt tctgtgtttt aacgcttatt attcgccttg
    17161 gttcaagagg attggtaaag tattcaccaa ttcttcttta cgtacttcaa ccatttcgcc
    17221 agttttcttg attttcactt ctacgattcc atccactgct tttttaccga cagtgattct
    17281 gattggacag ccaatcaaat cagaatccgc gaatttcact cctgcgcgtt catttctgtc
    17341 atcaaccagt acttcatagc cagcttttgt catcgcttct tctagttcat tcgtcaggtt
    17401 tgtttggtat tcgtctttta gattcatttg gacgatatgc aaatcaaatg gcgcaattcc
    17461 ttttggccag ttgattcctt gttcatctga atgttgttct acgattgctg ataataaacg
    17521 acttacaccg ataccataac agcccataat cacaaatttt tctcgtccat tttcgtctag
    17581 tacagttgct cccatagatt cactgtaacg agtgccgatt ttgaagatat gtccgatttc
    17641 gattcctcga gtaaaggcaa gaacaccatt gttgtctggt gaaggatctc cttcttgtac
    17701 aaaacgaaga tcttcataag aaagtacagt gaaatcgcgt tctggattca catttttata
    17761 gtggaatcct gtttcgttcg ctccagtaat ggcattcgct aaatcttgga cataacggtc
    17821 tgcatatact tttacgtcat ctgaaacacc gactggtccg acagaaccaa actcagcacc
    17881 tagatatttt tgtgcttctt cttctgttgc ttcttctaaa aagtcagcag ataaaaagtt
    17941 tttcagtttt acgtcattga cttcatggtc tccgcgtacc aaaacaagga caggttgttc
    18001 atccgcaatg aacaatacag acttgatgat tttttgaggt tcaacagaaa agaaatctgc
    18061 tacttcgtca attgatttta cgttaggtgt agctactttc tccaattcaa gctgtgtttc
    18121 atgcgatttt tttgatacat agtagcttgt agccatctcc aagtttgcag cataatcact
    18181 ttcagtcgaa taacagatcg tatcctcccc gatttctgag atcgccatga attcttttga
    18241 gtctttcccg cccattgcgc caccgtctcc gataatcgag cgaaattcta agccacaacg
    18301 cttgaatact tcggtgtagg ctttttcgta atctttatat gtctcgtcca aactttcttc
    18361 cgttgcatgg aaagaatacg catctttcat gataaactct cttccgcgaa gcaaaccgaa
    18421 gcgaggtcgt ttttcgtcac gatatttagg ttggatttga taaagattta aaggtaattt
    18481 cttatatgaa ttgatttcat tacgcatcaa atcagtaaaa gtttcttcgt gagttggtcc
    18541 aaggatcatt tttcgatcat tgcggtcttt gaaacgatac aagtttggtc catatgtttc
    18601 gtaacgcccg gattcttccc aaagttctgc tggcaagatg gcaggcatca gcatttctac
    18661 cgctccgatt ttttcaaatt cttcccgcat aatgcgtttc agtttttcca atacacgatt
    18721 tgctaatggc agatatgaat aaatacccgc tgatacttgg cggatatatc cagctcttag
    18781 caagatttga tggcttaata cttctgcatc atttggaact tcgcgtaatg ttggaatcaa
    18841 catttttgat tgtctcattc tatatactcc tttatttcct caaaatagtt atcgtttgtt
    18901 attataccct atttgtcaac tttttctaga aaaagaatcg ttgaatatcg ttccatgtaa
    18961 ctagcaccat caacaacatt aaaaatccaa agccagctaa tgtcaatatt ccttcttttt
    19021 cttgacttaa tggtttcccg cgaatccctt caaaaatatt caagactaat ttcccgccat
    19081 ctaaggcagg aatcggaagc agattgacga ttccgagatt cattgaaaga agcgccatca
    19141 aactaataac agttgttaca ccttgatttg ctgcttctga agacaattgg tacatcatga
    19201 caggtccgcc taatttatct aaactgaaac ctgtaaatag tgatcctaag gctttgaaaa
    19261 tttccaatga accgctgaaa gcttgacgcg tccctccaat gatcttatcc ataaaacctg
    19321 tattcattgg ggcttttata ccaagttggc caactttttc gccatttgac tctacagatt
    19381 tcggtgtcac gtcgacagat tgcacctgac cttctcgttc gatcttaaag tctaatgttt
    19441 tatccggatt tttcgtaatg actgttgtca gatcattcca tgaatggatt tcttttccgt
    19501 ccacactgac tacctcatca ttttctttta agcctgcttc tgccgcagct ccattaggca
    19561 tgatctctcc tacgcgatta gtgttcgtca cttgaacacc accttgcata aatgccagaa
    19621 tgatgaataa caggattgcc aaaataaagt tattcatggg tccagcaaag ttggtcagca
    19681 tacgctgcca caatttagcc gattggaact gtacgtcttt tggcgcaatt ctgatttctg
    19741 ttccgtcgtt ttcaataatc gtcgcatcgt gatcaacagg gtaggttact acttctgttt
    19801 cgtctccatt cacataacca gtgatagtca gctcatcttc taaatcataa cggctcaatt
    19861 ccatcgggat agcatttgtc aattggattt tcttacttaa atttatcttc tctacgatgc
    19921 cgtcagaatt tagtaaaaga gaaagaggca ttcctggtgc catttctgtt tcatcatccc
    19981 cattgcctgc cattcgaaca taaccgccaa taggtaatag gcgaagcgta tatgttgtcc
    20041 cgtcttttgc ttgatgccca taaattttcg gacccatacc gatagcaaat tcgcgaacga
    20101 ggattcctga tcgtttcgca aagaagaaat gaccaaactc atgaacaatc actaatatac
    20161 caaaaacgat gataaatgtc agaatcgttt tcatagcttt tttctccttt acttgttttt
    20221 tgtgtttttt ataaaacaac gataaaacac tataatttta ccatagtata ccaaagacac
    20281 caatagtatc aatataatga aaaataaaaa cctgcgtctt ttatgaaatt cacaaagaaa
    20341 cgcaggtata cttactaaat aatgccaaat aaatgcatga taggaaatac aaacagcaaa
    20401 ctgtcgaaac ggtctaagat tccaccatgg cctggtaaga tatttcccga gtctttcaca
    20461 tcataatgac gtttgattgc agattcgacc aaatcaccaa attgtccgac tgctgaaaaa
    20521 ataatcgtca tcagaagcat gacaaacata ccatgattaa atagttcttt tgctgggtat
    20581 agcaataaat aaagaaatgc gactacaaca gcactaccaa taccaccgag cgcaccttca
    20641 atcgttttgt tgggcgaaat atcaggactt aacttatgct gaccataacg tcgaccaacc
    20701 atatatgctc cgatatcagt tgcccaaaca ataaatagac caaatatcaa aacagccaag
    20761 ctttctgttc gtgcattgac aaaattctgg aaacctattc ccacatacaa gctgacaagc
    20821 acaggaaaac ctgcttcatc aatcgtatac gtatttttag aaataactga tgttcctaaa
    20881 agaatcataa ctataagata aaataacata aatgtactta cattctctgg aagaaagaaa
    20941 aaccatcttt cttttggcaa aacaagtaac actgcaccaa cggcggataa aactccttca
    21001 aaacttaaga gtgtcaatcc tttcatccga aacagctcgt aaactcctat tactgctaag
    21061 attgctgctg taagctcaac cgctatgcct ccgatccata taatcggaat aaacaagact
    21121 agcgccacgg ctgctgtgac cacacgttgt ttcatgactg atcccctttc tcatcttctg
    21181 ttttttttac accgccgaat cgacggtctc gattttgata agaggcgatt gcttcttcaa
    21241 gatgcgcacc atcaaaatca ggccaaagcg cttttgtaaa atacagctca ctatatgcga
    21301 tctgccataa cagaaaatta ctaattcgct cttctccact tgtacggatc atcagctcag
    21361 gatcttgtaa ctctttaggc aagaaacctg tcattaaatg cttagcaatc aattcatcat
    21421 cgatttcttc tgcgtgtatt tctccttttg ctacttcttc ggcaatttct tggacagcag
    21481 tcacgatctc tgctctgctt ccataattca aagcaaaatt caataccatt ccagtattgt
    21541 tttttgtttg ctcaattgca cgtcttacag catcttgcgt atgttctggc aatacgtttt
    21601 cataacccat gacatgaact ttgacatttt ctttaatcaa ttctggtacg aacgtatcaa
    21661 agaagtcgac aggtagttgc atgagaaaac ttacttcatc ttttggtcgc ttccagtttt
    21721 cagtcgaaaa ggcataaagc gtcaacactt tcactccaag ccttgatgct ttttttgtga
    21781 ctttctttac tgtttccatg ccttctttat ggccagcaac tcgtggcaaa cgacggtttt
    21841 gcgcccaacg accattgccg tccatgatga tggcaatgtg ctgaggaatt ggtccatcgg
    21901 tattaaattg aaattcgctt ttttcttgaa tatatttgtt tttttgcgga aaaaaacgta
    21961 acattctttt tcctcctata cttaaaagat tcccactatc ttaagtatta tagcaatatc
    22021 taactgtttt tcctatgaaa tcattgtttt tttatttaaa aactgaataa ttttttcttt
    22081 ttttaatttt taaataaaag aaatataaat aaaaacagag gaagcggaat gatccgcccc
    22141 tctgttttta tgattttatt aaacttccaa taattctttt tctttatcag cagcgatctt
    22201 atctacttct ttgatgctgt tatctgtcaa tgtttgtacg tctttttcta atccgcgcaa
    22261 atcatcttcg gtaatatcac catttttttg ttgtttttta tattcatcga tcgcatcacg
    22321 acggatgtta cgaatcgcga tttttgcatt ttctgcttct tttttaacat ctttagctaa
    22381 ttcttttctg cgttcttctg tcagttgcgg gataactaag cgaatgacat ttccatcatt
    22441 ggttggactg attccgatat cacttgccat aatagctttt tcaacatctt gaagaatgct
    22501 cttgtcaaaa ggtgtgatca ttaaaacacg tgcttctgga atttggattg atgccatttg
    22561 gttcaatggt gttggcgcac cgtagtagtt cacagtaata cggtccaaaa ggctagcgtt
    22621 cgcacggcct gcgcggattt gcccaagttc acgttgaaga ttttgtgctg cttttttcat
    22681 tttttctttt gcttctgtca taattgcatc tgccattttt atttccccct tacagttgtt
    22741 ccgatatttt cgcctaagat tgcacggcga atatttccgt gttcatttaa attgaagaca
    22801 agcagtggaa tatcattatc catgcttagt gaacttgcag ttgaatccat gacttgtaat
    22861 cctttggcaa taacttccag atgagtcagt tcttcaaatt ttactgcatt tgcatctact
    22921 ttcggatcag cagaataaac accgtctaca ttgtttttag ccatcaggat aacatctgcg
    22981 ccgatttctg ccgcgcgtaa tgctgctgtt gtgtctgtcg agaagtacgg gttgccggta
    23041 ccgcctgcaa agataacgat ccgtcctttt tctaaatgac gttctgcttt acggcgaata
    23101 tatggctctg cgatttgacg catttcaatc gaagtttgca cgcgtgtcgg tacacccacg
    23161 ttttccaacg tatcctgtaa agctaaggca ttcataactg tagctaacat acccatgtaa
    23221 tcagcttgag cacgttccat gcccatttgt gctccgattt gtccgcgcca gatatttccg
    23281 ccgcccacaa cgattgccat ttcaacgccc agctcgtgaa cttctttgat ttcttggatg
    23341 atttctttga ttactggtgg tttgattcca aatcctgcgt ccccagctaa agcttctcca
    23401 ctcaatttta agactacacg ctgatatttc ggtgtgacca tgttcattcc tccaatattt
    23461 tctattgcca ttttaccata cttagatgat ttcgacactc taagactggt ttttcttgtt
    23521 ttgcttaaaa aaagggagcg cacaaaccgt gcgttcccgt ctgttattca acagaattat
    23581 tttttcactt ggctcataac ttcttctaca aagttatctt cgcgtttttc gataccttcg
    23641 ccaacttcga aacgtacgaa tgttttaaca gttgcgcctt ttgatgcaac gtatttttca
    23701 acagtcatat caggatcttt aacgaatggt tggtccacca aagcgatttc tgctttgaat
    23761 ttcttcaagc gtccttctac cattttttca acgatattcg ctggtttgcc ttcgttcaat
    23821 gcttgttctg taagaacagt tttttcatgt tctaattcag cttctggaat ttgtgattcg
    23881 tttacataac gaggattgat agctgctacg tgcatagcta cgtctcttgc aactgtttcg
    23941 tcagttgttc catctagaac agtcaatacg gcaatacgtc cgcccatatg caagtatcca
    24001 ccaaatgcag cattgtcgtc tttttctaca acttcaaagc gacggaagct gattttttct
    24061 ccgataactt gagttgcttc gatcaagtca gattcaattg tacctttatc tgttttgatt
    24121 ttcattgcag cttccatgtc tgctggtttg ttttcagcaa caagttctgc aatttctttt
    24181 actaaatctt ggaacatttc gtttttagaa acaaagtctg tttctgagtt tacttcaacg
    24241 attgctgcag tgttaccttt cacagcaact gaagccaatc cttcagctgc aatgcggtca
    24301 tttttcttcg cagcctttgc cattcctttt tcacgtaaaa gatcaactgc tttttccatg
    24361 tcgccttcta cttcaactaa tgcttttttc gcatccatca ttcctacgcc agtcatttcg
    24421 cgtagttctt ttaccatttt agctgtaacg tctgccatgt ttaactcctc ctgtgtgttt
    24481 ttctttttaa aaaaagctgc ttcaaaggag agtgaggctt gtcgcccagc ctttgaaaca
    24541 gctccatcat tcaaaattat tctgctgaag aattgtcgcc ttcaacaaca tctacgattt
    24601 cttcgattga tgttgctgtg ttttcttctg tgaaatcttc ttcaactact tgatcttcac
    24661 cttggtttcc ttcgatgaaa gcatcagcca tttttgaagt aatcaattta acggcacgaa
    24721 ttgcatcgtc gtttgaaggg attactacgt cgatttcatc tggatcgcag tttgtatcaa
    24781 ccatagctac gattgggata tttaatttgt gtgcttcttg aacagcaata cgttctttac
    24841 gtgggtcaac gatgtacatt acatctggga ttcttggcat atcagcgata ccgcctaaga
    24901 atttttcaag acgttcacgt tctttgttca aaccagcaac ttctttttta ggtagaactt
    24961 caaaagttcc atcttcttcc attgcattga tttgtttcaa acggctgata cgtttttgga
    25021 tagtatccca gtttgtcaat gttccgccta accaacgatg gttaacgaag tattgtcctg
    25081 cacgagttgc ttcttctttg attgcttctt gtgcttgttt ttttgtacct acgaataatg
    25141 ctacgccgcc ttcttcagca acgctcttca tgtaatcata agctgcatct actaatttaa
    25201 ctgttttttg caagtcaatg atgtagattc cgtttctttc tgtgaagata tatttcttca
    25261 tttttggatt ccagcgacga gtttggtgac caaagtgtac gccggcttct agtaattgtt
    25321 tcatagaaat tactgccatg tttgtttcct ccgatttggt tttatttttc ctcttcttat
    25381 cttctacctt gccggcaaac tttttcaagc acctaccgac aatccaataa gaatgtggat
    25441 ttgctgatgc ataaagcacc ctcagctatt atacagacaa aagatccgta tcgcaagagc
    25501 aaatatactt ttctgctcaa cattcaatag tttacctgaa agataaaaag aattcaagaa
    25561 aaacagtaga tttctctttg tttctttatt atcctttttc cataaaataa ttttaggtat
    25621 aaaagacgaa tattattggg caatccttct tttttttgtt atgatacatg tgaaatcttt
    25681 cgtaaaggag aaagaaaatg cgcaaagctc aaagacattt gctgattaaa caagtcattg
    25741 aagaattccc gatccgtacg caagaagaac tattaaacaa acttgggtat tatggcgtta
    25801 ccgccacgca ggcaacgatt tctcgagata tccgtgatct aaaaatcgtg aaggcacctg
    25861 atgaaaatgg cgtttcccgc ttcgtattgt ttcgcgaaaa aggaactcct gagactagga
    25921 ctgaggaaga ggatcggctg gttcaaatga ttgaagacat tgtattgaaa gtcgaacgag
    25981 tccacttttt gacgatcgtc aataccttgc cagacaatgc tcatttattt gcatctgttt
    26041 tagatgagat caaacctccc tctattgtca gtacaattgc tggattcgat acgatcatgg
    26101 tcatctcgga aaatgaggag gacgcaaaac gagtagaaga atttcttcac catccaactg
    26161 aagtatcact tttttaatca gagcacgtat tcctaatatg gaatgcgtgt ttttttattt
    26221 tcaatatttt ccttattgaa taaaccttta cactctttac aaaaatacat cattttttat
    26281 tcaaattttt caacaccttg attgaatcac aataatcatc atttatattg gatgttaaca
    26341 agagcagctt cttgctcttt gtgtatttca aaaaagaaat gaaaggaaat catatgtata
    26401 aattattaac ttctggacca ttgacgactt ctgaaacagt gaaaaatgct atgctgatcg
    26461 atcattgtac ttgggatcaa gaatacaaag aaattacaca atggataaga aaagaattat
    26521 tggatcttgc tcaagtatcg gaaggaatat acacaacggt cttaatgcaa ggtagcagct
    26581 ccttcggggt agaatcggta ttaagttctg tgatcaggcc agaagatact gtactgattt
    26641 gttcaaacgg cgcatataga caacgaatga tcaacatgtg cgaatgtctg aaactgaact
    26701 ataccgttta tgaggtatcg gaacatcaaa tcctccaagc tcatgtgatt acacagctga
    26761 ttgaagagaa tccgtctatc tccgcactag caatggtcca tagcgagact acttccggta
    26821 ttttgaataa tttggaagag attgctgctg tcacgcaaga aagagatctt ttgtttatag
    26881 tggacgcgat gtcaagcttt ggtgggatac cgatttgtgt agccgaacta gggattgatt
    26941 ttttgatttc cagctcaaac aaatgtattc aaggtgttcc aggcttttct tttgtacttg
    27001 ctaataaagc ggaactggaa aaacgaaaaa atgttagccg aagtttgtca cttgatctat
    27061 atgaccagta ccgcgtgatg gaaaacgaga atggcaaatg gcgtttcact tcccctaccc
    27121 atacggtttt ggcttttcaa caagcgctga aggaactaga ggcagaagga ggaatcgaaa
    27181 ctcgatattc acgttatctc gccaataatc atgtgctacg acaaaaaatg acagagatgg
    27241 ggttccagcc tttcattcaa gaatatcagg ggccattcat cacctctttt ctttacccta
    27301 aggactggcg atttgatttt aatcattttt atcagtattt aaagcaaaat ggatttgtga
    27361 tctatcccgg gaaaatcagc gatcatgaat gttttcgtat cggaaatatt ggcgagatct
    27421 atccggaaga tattttggaa ctgacagcaa ttatcaaaga ctataaggag aacgttaatg
    27481 attaaaactg taatttttga ttgggcagga acaactgtgg actttggctg tatggctcct
    27541 gtccacgctt ttagaaatgc ttttctagaa aaaggaatcc aactgactga caaagaaatc
    27601 cgctaaccga tgggaaaatt aaaatgggat catatccaac agcttctttc cctcccttct
    27661 gtcaaaaaac aatggatagc tatttatgga caactacctc aggaaaaaga ggtggaggaa
    27721 ttgtatcaac aatttgaaca ttatctgttt aaagatttgg tcaagcattc taccttgaaa
    27781 cccgacacat tggaaacgat cgaacggttg agaaacgctg ggatcaatat tggttccacg
    27841 aatggattta atgctcctat gatgacaatc gtagcagaaa ccgcaaaaaa agaaaggtat
    27901 tcaccagact tcatggcaac ttttgaagac gtcgaagggt atggtcgacc gtttccttat
    27961 atgatccata aaaacatgca gtacttcaaa aacaagtcgg ttgatgaagt aatcaaagtc
    28021 ggagatacgg tctcagatat ccaggaaggg aaaaatgccg gagtgttgac agtaggtgta
    28081 attgaaggta gttcgctaat ggggatttct tatgatgagt accaaaaatt agagatgggg
    28141 aaaaagatcg tttaaaacag tctgttgctc ttagattcca acaagccggc gctgatattt
    28201 gtgtaaacaa tttaaaagag ttaacagaat acattttgta cgctgacatg aatccagaat
    28261 gacttttata caaaataaga ggttgtgaaa aagctgtcct gtatcaagta ataaaaacag
    28321 ccaagcaaaa atagctcctc atatttttcg tttggttagg cttattaccg cagatgcaag
    28381 cttttgaaca ccgtttattc gaaaaaaaaa agggagctgt gacacgactt ttgtcacagt
    28441 ccccttattt gcaaacaaac agtgtttaag agacaacctc tccactaaac ggtgttcatt
    28501 cagctgactc tcggtatttg ctcaaaaaac gataaaattt gaaaagctat tttctctttt
    28561 gtttctctaa tactcggatc agaacgctga tttcacattc tctttttctt acatcttatc
    28621 tggtgcatga agtcctagca aacgaaggtc ttctttcaat aatacagtca ctgcataaac
    28681 tagagctaag cgtgcttctt tttgttcatc atcagctaaa atcttcgtat gagcatagta
    28741 tttgttgaat gcttgtgcta atttgattgc atgtttcgca atcactgacg gttcgtattt
    28801 ttccccagca gacaagaccg tttcagggta tttttgaact aatttaacca cttcccagct
    28861 ggtatcgtca ttcaatgcat agtctgctgt ttctgaaggg acaaatcctg ctttttccaa
    28921 caggctgact gcacgagcat gtgtatattg cacataaggt ccagtttctc cttcaaatcg
    28981 aacaacttct tccaggttaa agtcaaatgt attcagtcga tcgtttttca aatcgtggaa
    29041 aatgactgcc ccaaccccaa cttgtttagc tacagcatct ttgttttcca aatcagggtt
    29101 tttctcgctg atttgttctt tagcagattg gattgcttca tttaatactt cttctaataa
    29161 gacgatcttt cctttacgag ttgataattt tttaccacct tgcgtgatca aaccaaatgg
    29221 aatatggtgc atgtcgtcag accagtcaaa gcccatttcc ttcaagacag ctttcaactg
    29281 tttaaaatga tagctttgtt cattccctac tacatataga gattgtttga agtcgtaagt
    29341 acgtttacgg tacaatgcag cagctaagtc acgtgtaatg tatagtgtcg caccgtctga
    29401 cttcttaatc aacgctggat tcaggtcata agcagatagg tcaacgatct ctgcaccttt
    29461 atcctcgttc agcaaatgtt tttcttctaa aagtttgacg atttcatcca tcttgtcatt
    29521 gtagaaagct tcaccgttca acgaatcgaa acgtacttcc agcagatcat agattttatt
    29581 gaattctttc atcgattcat cacggaacca ttgccataat tgaatagcct ctttatcgcc
    29641 ttcttccaga cgtttgaacc aagcacgagc ttcttcattt aattctggtt ctgtttcagc
    29701 tacttcatgg aattggacgt ataaacgaag caattcattg attggttctg ctttaacagc
    29761 ttcttctgta ccccattttt tgtaagcgac gatcagtttt ccaaactgtg ttccccagtc
    29821 acctaaatgg ttgatgcgga ttggctgata accgattttt tccataatga aaccgatcga
    29881 attaccgata actgtcgaac gcaaatgtcc cattgagatt ggtttggcaa tattaggaga
    29941 agacatgtcg attggtactg ttccttgatt cccgatgttg ctatccccat aatgttcttt
    30001 ttctttcact actgtttgca aaactttttt gctgataagt tctttattca tgaaaaagtt
    30061 caagtaaggt ccgacaactt cgattttttc aaagttggca gaatcgattt tttctgctaa
    30121 atctgcagcg atttgctgtg gcgcttttcg gtaaactttt gctaatgaaa aggctggaaa
    30181 tgcaacatct ccgtgttcag ctgattttgg attttctagt aattgctcga cttgttccag
    30241 tgttaaatca tctttgacag cgtcatagac agcttttgcg acaagatctt tattattcat
    30301 ctttattcct cctaaagtgt tcccttgatt gttaaacaaa aaaaatctct agcgacggca
    30361 tacttagtat gccatcacta gagacgagat ttcccgcggt accactccaa ttgtcctaaa
    30421 aaaggaccag ctccatctga tatcggtaga cgaccgtctg tttaaaacag aattctccaa
    30481 gtgcgcttcg ttgtatcttc ttcattcggc ttccaccact tccgaattcg ctagccaaga
    30541 atgatacaag tactctcttg ttcaacaaat cttagtctta ttaaactttc ttgaattata
    30601 tcagaaaaaa aaacgaaagg ctagtcgttt cataaaaaaa caatccagat ggcctaaagg
    30661 agaagaaata ccacctggat agctataaat catttcgtaa atacgaaaga cttaacaaat
    30721 taatcagcta cgataatcgt tccttcttca gaagctaaaa gattctcgat attttctaaa
    30781 gaagtgatga ttgccttagc gtttggtttt gcttcaacaa atgcaatagc tgcttctact
    30841 ttcggaagca tgcttcctgg agcgaattgc ttttcatcaa tgtattgttt catttctgaa
    30901 acagtaacag tttctaattt cttttgatct ggtttttggt aattgacata cacattgtct
    30961 actcctgtca atacgatcag caaatccgca tcgatgattt cggctaattt ttctgaagca
    31021 aaatctttat cgattacggc ttctcgtcct tctaatccag tagctgtttc tacaacagga
    31081 atcccaccgc caccaacaga gactgtgata actccttgat cgaccagcgt ttcgatgaca
    31141 cgtgcttctt taatcgagat gggtttaggg gaagcgacta cttttctcca tccacgtcca
    31201 gcatcttctt tgaatgtcac agtggagtca gcattcattt gttctttggc ttcttcttcc
    31261 gtgtagaatg gaccgactgg tttacttgga tttttgaatg aaggatcatt ttcatccaca
    31321 ataacttgag tgactaaaga aaccacatct ttatcgatcc ctttttcctt cagtacttcg
    31381 cccatggcgt tttgtaacca gtaaccgata gatccttctg tcattgcaac acatgtatct
    31441 aaaggcattg ccggtgtttt ctcagaatca gcagcttgct gctgaatcaa taggttaccg
    31501 acttgcggac catttccgtg ggagatgatc aattcatctc cttgttcgat aaatttcact
    31561 aaatactttg ctgtttccat taacgcttct tgttgggctt ttgcacttgc atccgtagac
    31621 aagatggcat tgccccctaa agcgacaact acttttcgtt tggccatttc attcgtcctt
    31681 tcttcagttc taccgaattt tctagataac aaaacgagcc agcgttttgt gattttccct
    31741 caatgctgac tcgttttttc gtgagatgaa tgtggttatg cttaaacgcg agggataaac
    31801 aagttaccta atgttgctgc catgatcgct ttgattgagt gcatacgatt ttctgcttgg
    31861 tcaaattgac gagcatattt gctgcggaag acttcatctg ttacttccat ttcagtgatg
    31921 ccaaagcgtt ctttcatttg ttcaccgtaa acagtgtttg tatcatggaa agctggcaag
    31981 cagtgcaaga agatcaaacg atctgtgttg tgtgtttttt ctaccatttc catattgatt
    32041 tggtaaggtt tcaacaattt gatacgttct tcgaatttat cttcttcacc cattgatacc
    32101 catacatcag aataaaggac gtctgctccg tctacacctt tatcaacatc gtcggtaatc
    32161 atcaattgtg caccggattt ttcagcaaag ccttctgcca ttttgacgat ttcttcttca
    32221 ggttgtaatt cttttggtgc aacgatacgc atattcgctc ctaaaattgc acctgtgact
    32281 aataaagagt tagccatatt gtttcgacca tctccgcagt aagcaacagt gataccttct
    32341 actgttccaa agttttcttg gatagttaaa aagtcagcga tcatttgtgt tggatgccat
    32401 tcatcagtca agccattcca aactggaaca ccagagaatt ctgctagttc ctctaccatt
    32461 ttttgactga atccacggaa ttcgattccg tcaaacatac gtcctagtac tttagctgta
    32521 tcttctgtgg attctttttt acctaattgg atatcatttg cacctagata ttcaggatgt
    32581 gcacctagat cgatcgctgc agtagtaaat gcagaacgag tacgtgtaga tgttttttca
    32641 aataaaagcg caatattttt tccttctaga tagtgatgtg ggataccacg ttttttcaag
    32701 tctttcaagt gttctgaaaa atcgatcaaa tattgtaatt cttcttttgt aaagtctttt
    32761 tctgctaata agcttcttcc ttggaatact gattctttca tattaaacat tctcctttag
    32821 attgttttta tttaagatta aagaggaaat cccgacggca gctgacagta agactgccgt
    32881 caggaaacaa attacttcaa gtcttcacgg acaagtggtt ggctcatgca gcgtggacca
    32941 ccacgacctc gtgacaattc acttgaattg atttctaaca ctttgatgcc atagctacga
    33001 agcaattcgt tcgatacata atttcggtta taagtgacta caacacctgg agcgattgcc
    33061 aatgtgtttg atccatcatt ccattgttct cgaggtgcaa cgatctcatc tccattacca
    33121 gttgggatca gtacaagatc atctaatcca agagcagctt tcatcgtttc gtgtaagtta
    33181 tctgaatgag taatcttgat atcatcacca tctggttcga ttgtaaagac atccatctta
    33241 ccgtctttac tttgaattgc tggatggata gtgaatttat cgtgatctac cattgtaaat
    33301 acggtatcca aatgcatcat tgcacggttg ttcggaattt tgattgccaa cactttttca
    33361 aatcctgaat tttttgcgaa taggtggcgt gctaattttt ctaaagcttt cgcattagtt
    33421 cgttgagaaa tcccaacagc tactacttta tcgcttaaga tcaattcgtc tccgccttcg
    33481 atatgatctg gattttcacg atccaaccat acttcgattc ctttatttgc aaatcttgga
    33541 tgatgtttca aaatatattc tgtaaacatc gattcacgac gacgtgcttc aaaagtcatc
    33601 ttattgactg tcatcccatt tcccattgat gcggaagggt cgcgtgtaaa gtacaagttt
    33661 ggcattgggt ccatgtaaaa tggatagtca tcgtctgctg acagatcata tagactgcta
    33721 gaacgtactt cgatatcttt tgtacgaacc ccagccatga ttttatctac catttcttgc
    33781 gtttccattg atagaaggaa ctcatgaaga ccctctctta cagcattcga tgcaatatga
    33841 gattcatcca acattttatt caagaattgt tctttgacat tgcctgcgtc aatcgcttct
    33901 gccgctaatt tttccaagta aagagtctcg acgccttcat tttgcaatgt cttcgcaaag
    33961 ttgtcatgtt cttcttgagc aatcggaaga tatggaatat catcgaacaa cagacgatcc
    34021 ataatatctg gtgtcaaatt ttccacttct tgtccaggcc gtttcagtaa aactgtcttt
    34081 aatttcccta tttcagagaa aacgtgaata ggtttatcca tgttgtgttg cctccttctt
    34141 tttgattaca tgtataggat atcctccttt tgtaatcact ttcataattt tgtctcattc
    34201 aaaacgagac attcctcaca ttaatattcg ataattaaat aattaaacgt ttttctattt
    34261 ttaagcgtaa tttattcatt ttttattctg tatacacttt caaaaaaata tttattcatt
    34321 gaaaaaattt ttttgaataa attaaaaaaa taaattatat tatcgttgtt agcgtttact
    34381 atatgtatta ttgttgtttt ttatacacaa atcattcgct tcaaaacaaa ttattcaata
    34441 ccattgtata ttttttacat aataacaaat tatatttgta aagtattaaa acttgtttct
    34501 aatgttggta tgttaaaact ttacaaattc tttaaaaaaa gtgacaataa atatagtgaa
    34561 caataaggag aggacttttt aatgcgtgtt aataaaatcg attttcactt ttcaaaactc
    34621 agatatcaac ctgattttga acatttctcg gatgaagagt tccaacagat caaggaacat
    34681 actgttcttc gttcttataa aaaaggacag atccttttcg acgatggtga tcccagacaa
    34741 cggttgtata tcttgaccaa aggactcgtt cgtttagaac gttatgatga aagtgcaact
    34801 tattactact atgactatgt gaatacaaat aacctttttc ctatgtcagg aatgtttgga
    34861 gaagagacct acacttatac agctcaagcg atgactgata ttgaaacatt ctaccttcct
    34921 gtttcctact atgaaaaatt agcacgaaaa aatcccgaac agttgatcta tatgatacaa
    34981 aaaatttcta aagtagttga aatacatgaa ttgcgtatcc aaatcggatt gacctctagc
    35041 gcctttgacc gggtaaaaca aaatttattc attttgaaag aagaattggg cgtaccaaca
    35101 aaagatggtc atatcaccat tccttatccg atcaccttaa aagaattagc agtgaatagc
    35161 ggcacgacac gtgaaactgc tggacaagta atcaaacaac tacgtgattc cgggctgtta
    35221 gattatgaca aaaagcgttt tactttctgt aagacagcta ctactccagc tatgagagaa
    35281 taacgcgaac aaaaataagc agtgaccctg ataaatatca gggtcactgc ttattttcct
    35341 ccctaaaact tctgaaagat ccggcagttt tagatcactg gacaagactc aaagattttc
    35401 tatgtgtgtt tcagttatgt aatattcact gtttgcaaaa aatactgtta gattaactag
    35461 atgaagcgtc ttgtcctcat ccgatctaga ttattcgata ttcctcgaca ataaacgatt
    35521 actcagtgag ggcaatcaga ggaacggata atgataaata agctgtaaaa aatagcaaaa
    35581 taacaataat gattaaaagt atgtaaaaaa tagggctttg acaaaaacgt gtcacagccc
    35641 tatttcgatt aaacggtgtg tacctgtcaa ctcttcggtc acaagtcata atgttcataa
    35701 acatgaggag ctgttttttg aaattgttcc ttgtgaatca gagttaaacg acaggcttac
    35761 gaactcttga ttaaacggcg ttcaaaagct tgcatacgcg gtaataagaa cagccaggtc
    35821 aaaaacatga gaagccgttt ttcctgggct gttcttatta cttgatgcag ggcagctttt
    35881 tcacaacctc tttattcaga taaaaccaag cttggatcag cattcaactg atagctagca
    35941 aaatttcttt tttgcatttc cacatgtgct gctgctccaa tcattgcagc attatctcca
    36001 caaagtcgaa gagggggaat cactagttct acctcaggga gtttagctgc caaagcatcc
    36061 tgcaatcctt cacgcaaccc ttgattggcc gctacacctc cagctataat cagttgcttg
    36121 actggataat tttgacaagc tcgaagtgtt ttagtaatca atacatcgat cacacttgct
    36181 tgaaagctag ctgctaaatc atttttgtct agatcttctc cacgctgttg tgcattatgc
    36241 accaaattga taaatgcact ttttaatccg ctaaaactga aatcataatt atcttcatgg
    36301 atcatcgctc gcggaaaatg gtaattatct tttccctgat gagctagctg atcgatttct
    36361 tttccgcttg ggtaagaaag acctaatact cttccaactt tgtcataagc ttctcctgct
    36421 gcgtcatctc ttgtctcccc aatgatttca tatgatccat cttcttgcat atagactaat
    36481 tctgtatgtc ctccgctaac aagaagtgcc atcaatggaa actggaatgg tttaaccaga
    36541 cgggctgcat agatatgccc tgccatatgg tttacaggaa taagcggcaa ttggtgtgcc
    36601 caagcaaaag ccttagctgc agaaatacca atcagcaacg agccgacaag tcctggtcca
    36661 taagtgacag cgactgccga taaatcttct gccgacacac cagcttctac cagtgcatct
    36721 tccaagcata aggtgatttg ttctacgtga tggcggctag ctacttcagg tactacgcca
    36781 ccaaaacgtt tatgactgtt tatttgagaa gcaacgatgt tagataagat ctctgttcca
    36841 tttctaacga ccgctacact cgtttcatca caacttgatt caatcgccag aatcaattct
    36901 tcattcattt catcgtctcc gtttttaact ttctgctcat aatgatcgca tcttccgtcg
    36961 gattgcgata atatgatttc cgtttaccta atatacgaaa cttttctgat tcataaagtt
    37021 ttcttgctgc ttcattagat agacgtactt ctaaaaaaac atgcagcgaa tcttttattt
    37081 gttccgtaat cagtaattgt aacaaatgcc gtgcatgccc ttttccttgt tctgaaactg
    37141 caacagcaat atttgtgatc tcgacttcat ctattacttt cgagtatcca ataaagccac
    37201 agatctggtt atcttcagct aaaagcagat aatcagagtg aggctgttgg atgtctgcca
    37261 aaaactgcat tttcgtccaa ggtgaaccat gtggataagc attttcgcta atcatccata
    37321 cgcattctgc caagtacgct tcatcaaaat ctttttttct gcaaatcaac taatctcact
    37381 cattttctaa aagttttatc atatccaacg catcttcttt tgtttcagtg taatatcttt
    37441 ttttgatcgt tgttgcttga aaccccaatt tccgataaag tctttgtgca tcttgattac
    37501 tcatacgcac ttctaatgac atcgtctcac agcgattcat tatagcaaag ttctcgattt
    37561 cgtcaataag cagcgaaccg attcgtttac gctgatatgc cggtcgaacg gcaatattcg
    37621 tgatatggca atctaaaccg aacgctcttg aaccgataaa accaatcatt cttccattat
    37681 caaagatacc tagatataga tgaggaagcg gtgacttcaa ttctgatata aaggcacttt
    37741 ttgtccatgg caattcgcca ttatagactt ctcgttcgac atccagcagc tctttgatat
    37801 cttctgtagt caattctctt agtacgtaac gacgatcgat ttgttcgact gttttttctg
    37861 tataaatcat tttatcttga aaaaagcttc tggcatacgc tctaaatttt ttcaacataa
    37921 ctttcgtctc caggtgtgtg cgtctccaac catttttctt ctgcttctac tcgttttaaa
    37981 tagcgaggaa gaaaagcttg tttctctgca actggttcaa ctaaagcacc taattgagcc
    38041 acagttacac cacttggaat atcccatgct ggaactgtat taatcatcgc actaggcaat
    38101 atttccttta tttcagagaa gaatttgtga caatcactcc cgacgaaata aatagcttca
    38161 ttcagctttt gtagttgtgt gaacaaatca gaagcagaaa tatgtacatc ttcaagtaca
    38221 gtttctaatc tattttcttt ccagcggtat gccccagcgt atacattttt tcttcgagca
    38281 tcaaacatgg gcacaataat attatccaca ccgatgcaat tagccgcgat ggttttcaga
    38341 ctagatactc ctactagctc tttatctaat gtataagcaa tcgtttttgc tgtcgttaca
    38401 cctatacgaa gccctgtata agaaccggga ccatcagaaa ccgcgattcg gtcaatgtct
    38461 tttggactta tttgaagatc tgcaaacaat tggtcgattg caggcatcag tgttacactg
    38521 tggttgcgtt tgatatttgt ttgtatttgt ccaagaattt gttcgtcttc taccacaccg
    38581 atcgccaaag tttggtttgc agtatctatt cctaatgtaa tcatatattt gatcctttct
    38641 gctatgacat tctcgccttc cattgtagca tactttttta ttctgaggat gaaaatacca
    38701 aaggcaaaac tagtacaata atacatgatt atctgctggc gggcaaattt tgccccttcc
    38761 tgaaacacaa aaaagctgcg gtatcactcc attttcccac agcttttcca tcttttattt
    38821 cgttgattct cgaataatca actctgtttc cacaataatc tttcttgaaa ccggtgcttt
    38881 ggaattactt aattgaagaa tcgtctccac tgctagctct cccatccatt cggtataaac
    38941 ttttacagta gaaagcgagg gcgttacata tttcgctaca ctgacatcat tgaagcccat
    39001 cacagaaata tcttctggta ccactaaacc tttttcttga atcgctttca gtgcgccaat
    39061 cgctaatgca tcattagctg caaaaagcgc atcaggaaca tgcggattcg tttttaaata
    39121 ttgcttcact gcttgatatc ctgactcgac agaaaaatct gcttcgataa ttggaccttc
    39181 gtcgtagcct ttttctgcca aaacttgttg aaatgccata attctgggat cttttagccg
    39241 ctgatggttt ttcttcgtat attctgttcc agcaagcata gcgatatgtt gcgctttatg
    39301 ggagacaaag tgttcaatga ctttttgaac acttgactta aagtcgacaa ctagagagtc
    39361 gattccttga gaagtgccgt cagaatctac caaaagcaag gcattttggg atcctttcat
    39421 ttctaataac tgagcttcgt cgaattttcc tagtgcgatc gttccatctg ccggaagatc
    39481 actaatggaa tcccacgttt ctttcaataa agtaacgttt gcttcttctg cctttttttc
    39541 gattcctagg cgaatagata aataataaag atcttcaagc tcttccgtat cgtcgtacca
    39601 ctggatcagg cgaagcgttt gcttagtatt tgctgcattt ttacgatgtt tcgtataatt
    39661 taagcgttct gctgcttcaa aaattttttg ctttgttgct aagccaacgg aaagatcagg
    39721 atcatagttg agaactcttg aaacagtagc aggagaaact cctgccaact gcgcaatgtc
    39781 tttaattgtt gccatttgtc tctcctcctt ttttacgttt ataaaatcgc agtaaatcgt
    39841 tcaaacgctg cctgtccttt ttcatctcgc ttgaatactc cagcgtcttc cagtacacgt
    39901 gcaaaaacct gacctacaga tttttgtacg acatcggtga ccgtatcttt cgtcaaatca
    39961 ggatgttctt tttttaattg ttctgcccac ggacgatgat attcagccat ctggttttct
    40021 tctcctaata agaaatgctc tacttctact agttctggtt tcaaacgagg tggtaaaaca
    40081 gcaagcccca ttacttcgat caacccaatg ttctcctttt tgatatgttg gacatcttta
    40141 tgtggatgaa aaatcccatc aggatgttct tctgaaacat tgttgtctcg aaggacaagg
    40201 tccaactcaa agtaattgtc ccttcttcgt gcaattggcg taatcgtatg gtgtggtgta
    40261 ccatctgctg aaaaagctct gatagatact gtttcatccg aatatgcttt ccatttttcc
    40321 aatactgagt tagctgcttc aataagcaag tttttctctg ttgattgcaa acggatcaca
    40381 gacatcggcc atttcacgac tcctgcgatg acttgcggat aatcttctag tttgataagt
    40441 ttttctatcg gtgcttttgc cataggaaat tcatgacgtc ctgcctgata atgatcatga
    40501 gaaagaatcg atcctccaac aattggtaag tcagcattcg aaccaacaaa gtaatgaggc
    40561 aagacttcag taatccgaag cagtctagca aatgtttcct ttgtgatttt catcgggcgg
    40621 tgttgttctg ataaaatgat cgaatgttca ttgtaataag cataagggga atattggaat
    40681 ccccagcttt caccgtctag attcattcga ataatccggt gattggtgcg ggctggatag
    40741 ttcaagcgac ctctatatcc ttcgttttcc atgcaaagca tgcaggtagg atagtctact
    40801 tttaccgcct cgcgttctgc tgcgatttgt tttggatctt tttctggctt agataaattg
    40861 attgtgattt ccagttcacc atactccgac tctgccggaa atttgatgtt ttttgcgatg
    40921 gctcttgttt tgatgtagtc atttttctta cttaaattaa agaaatactc agttgcttcc
    40981 tctgggcttt tcgaatagtg ctgtgcaaag aatgcattga cgactgaagg tggcggtgtg
    41041 agaaaatcca ttaattgcgc ttcaaaaatc tcttgttctg ctggcaattc tgaaagaaca
    41101 ccattttctc ttgccttagc gacaagctga tccaataagt ctacagattc ttccttaact
    41161 ggtcttactt caatgctttc tagagcatct tctcctacca tccctaaaag acgattctgt
    41221 aaatataagc ggtccatttc catccatccg ccagcttcga tcgcaagtgt ggcaaaatca
    41281 caaatcgttt gactgattga catatctcat tcccctattc ttcataacca tttggatggc
    41341 tgacatgcca atcccatgct gttttgataa tcgctttgat atctgtgtat tcaggtttcc
    41401 agcccagaat ctctctcgct ttttcacttg aagcgactaa tcggctagga tctccagctc
    41461 ttcttggtgc tatttctgct ggaatttctt ttcctgtcac ttctctagct gcttctagca
    41521 tttccttgac tgagtatcct ttgctgctgc ctaaattgaa gaagttgctt tcatttcctt
    41581 ctttcaagta ttccaaagct aaaatatgtg cagcaatcaa atcttctact tgaacataat
    41641 ctctgataca tgttccatct ggtgtatcat aatcgtctcc ataaacagcc aaggcttttc
    41701 tttgaccaag agctacttgt aaaatgattg ggacaagatg cgtttctggt gtatgatctt
    41761 ctccgataga tgcatctgct tttgctccgg ctacattgaa gtagcgcaaa gcgacatagc
    41821 gcatgccata tgcttggtca caccacttca tcattttttc catcatcaat ttactttcac
    41881 catacggatt tttaggatta gctggtgtat cttctgtgat tggagactct ttcggctctc
    41941 cataagttgc tgcagtagaa gagaatacga ttttattcac gttgaactcg tgcatgactt
    42001 caagtaagat ttgcataccg tatacgttgt tgttgaaata catcaatggt ttttcgacag
    42061 attctcctac taaagagctt gcagcaaaat ggattactcc ttcaatcggt tccttttcaa
    42121 atacggaacg tagaaattct ttatcccgga tgtctccttc ataaaaatga gcatctggat
    42181 gtactgcttg tttgtgacct gtgagcaaat tatctaccac agctacttga tacccctttt
    42241 gaactagctg gtctacagcg tgcgaaccga tataaccggc tccgcctagt actaaaattg
    42301 tcataaaaaa cactcctttg attagtaaaa ttgtgtttgt tgattctata taatttaaaa
    42361 aacgaagttt acttttatgg tgaaaacaaa caaagtaaaa tttcgtaaat tcatatggta
    42421 aatttataat aaaacattta gtaaacattt tcaagggttt tttgcaaagg tttacaaaca
    42481 aacgtaaaac tagccttttt ttctcttttg ttccataata gatataacaa ataataaatg
    42541 aggtggattc aatgaaaaaa tttgtatctg gaatattagt aggaagtctg gcaacagccg
    42601 ctgccgttgc tggtttggtt gcttctgtga aaaaaacagt gatcgatcca attgacgaaa
    42661 aagaagcaat gatcgaagaa aaccgtaaaa aagcaatgcg taaacgcgtt tctcgttaat
    42721 aattatttaa aaatacgcat gaagactttc taaaaaacag ataagtcatc atgcgtattt
    42781 ttatttgtca gtcaatataa gttgtgctat tttatctgcc tgtctcagat tccatggatc
    42841 tgtataaggt ctagctactg ctgcagttag ccaatccaca ccttcaacag gtattccata
    42901 gcaataaaag tcagactgga tttcccctga agcatcaatc acgcgattcg tacctcgctc
    42961 aacatctata gcttctgaat gataacttcc tgtattatct gaaaatgaaa aagggcgaac
    43021 aagtttgttt tccttcaaat tttttatgac aggattactt gaacgagaaa agcttgtttg
    43081 tggcaatcga gcttcaatca aatagcgact gctgatttct ttatcttgta gagaaataac
    43141 atagcggttg tgcttttgct gaatatcaaa tggcggttcg atcaaatgaa aaatacccgc
    43201 atctatcagt gctgctaatt ctttcgtccg aatgattggc ggaccgatcg ttaaaaagct
    43261 atttagatga gtaaaccatc cccatagtat ttctttgtat tccttagcag aaaaaacgtg
    43321 ccattcaata gcctgccgga ttggatcacg ccagtctttt aatgcatcaa atgttgaagc
    43381 aaatgctcct gtgcaattgc ctttttccgc ttcttttatc tgcccttcaa taaatattcg
    43441 gctagcttct gaaaagggct cattctgata ggcaagagga gtttctaaat acgaccaatc
    43501 ccatctataa gggattagtg ctggatgctt tccgagacac tcctctgaag agagggagag
    43561 aaaatcatgc tcaaaatttt tctttggaat cggcaagtga tattcttcga tgattttctt
    43621 gtaataaaac aactcaacgt cttttttcag atagtcaaaa aatgtttcgc cagtcaaaaa
    43681 tcccgactga tgccattgct ccaggttttc ttttgttagt aaccgtggcc atgccattgc
    43741 tccgccttgc ttttgatttc ttcctcgagg gtaataaggg agcccccttc ttgatccact
    43801 gtatacaatc ggttccttgc ctgatggatg atagaccagt tttccctttt cgttctcgaa
    43861 gcgccctcct cttgatactg taaacaatcc gacataatca aaaaaactta agcctagacc
    43921 tcttaaaaag acattttctt taggcggtat cttcgaaaga tctacatctg caggattaga
    43981 aggaagctga tacagtaaaa catttttctt tgcgtagtct acccacactc tttcttcttc
    44041 tgtctcatca ttttcccagt gaccgcttgc taaaacaagc tgatcgatag aatagctttg
    44101 ctgatctgtc tgtaaaagat aaccatcttt tcttttgctt acttttttta ctaaagaata
    44161 aacaaactga atagaagctg gaaattcttc ttgcaacttt gagaaaaacc agcgttgata
    44221 gagtccgtat aaacatcgag tagattgctg atctttttcc agacgattga tttcttctat
    44281 aattagttgc ttatttttgg ttaccgtctg ttcgatataa tctgtagcag gtcctttact
    44341 ccattgaaac aagttgggtc cctgtgttac caatcctcca ctggacaatg tttcatctgt
    44401 aaaaagcgtc acttgttgag aaacagaatt cattaataac tccggtggct gattcaaccg
    44461 ccaaactcga cctccaggac cattgggatc gaaaagaaaa agttcaagag aatgtctgtc
    44521 ctgattatta tggagaatcc tttctgctac acttaatcct ctaggtccgg caccaatgat
    44581 ccctattttc atttgcatgc ctccatttcg ataaattttt tcaataaaac agcatggttc
    44641 tgatgttcat tttttgcacc ataaacaaaa gtaatgactg gatgttcttt cataagttga
    44701 ctgagtttaa aaatagcttg cttcttttct tcttcgaaaa tcaattcttg ctggtatttc
    44761 tttgcaaatg aagcaaatcg ttctggtaca tggtcaaaag ttttgcgtgc ttcattacta
    44821 ggtgcaattt ctttcaacca cagatctaac tgcgcttttt cttttgaaat tcctcgtggc
    44881 cagaatctat ccacgagtat tctatagcca tcctcttttt cagcatcaag gtaaacacgt
    44941 tttaatttga tcatagttgt ttccttcttt ctaccttttc gcttagctta tatatgtaga
    45001 taatagcaca aaataaaaaa aatgaacggc acaactatcc attgcgccgt tcatgtgatc
    45061 ttggtcaaaa tctaatgatt gattcggttt ttaaagctgt catacaatca tctgttttat
    45121 acttttttac ttttgatttt tccagaccat ccgtcatatc cacccttaag taaataaatc
    45181 tcttgataac ctgctttacg taatttattt gctgtacgga tgctcaaact tttcttttga
    45241 tcgtaaatat agacaggctg gtcttttcga atagatccta atgaatcttt caaaacagtg
    45301 taagggatgt ttcttgcacc taaaatatgc ccagcatcaa acacatcttt ttcgcgaaca
    45361 tcgattactt gtgcttttct cattccttct cgaaattctt cttctgtcaa agtcgttgct
    45421 gaacgtttgg ccattactcg tagatagact tcccaaagga tgattgcaag cacaatcaat
    45481 agcaaaatga tattaattac ccataaaacc atgtaatgcc tccgtttttt taagcaaatt
    45541 tcaaggctaa tgaggcagca ccaatgacac cagcttcatt accaagctct gctaatttga
    45601 tttttgttga ttctcttact tgcgggaaag taaattcttt aaaatatttc tctacacggc
    45661 tacgtaaaaa ttctcctgct gccgatactc ctccacctaa cacgatactt gacggattta
    45721 atgtattccc caagtttccg caagctaaac ctaagtagaa gcatacgcga tcaacaacca
    45781 tcaatgcaaa cggatcgtct tcttgagcca tttcaaagat atctttactt gttacttctt
    45841 ctccgttgtc tagcattgct ttcagttttg aatcccctgc gtattcttct gccaagaaac
    45901 gacctaggcg taccacacct gtagcgctag atacagtttc taaacatccg cgtttaccgc
    45961 aagtacattc gaacccttca ggatccacag tgatatgtcc gatttctcct ccagctccgc
    46021 tcacgccgtg aatcaaattt ccgccagcaa cgatacctcc gccaacacct gttcctagag
    46081 taacgaatac aacgtctgga tcgttttctc cagcaccttt ccatcgttca cctaaagcag
    46141 caacattcgc gtcattatcg atagcgaatt tgattcctgt tcctgcctcg atcagttttt
    46201 tcacaaattg aacttctgtc cagtttaagt tatacgcacc aataaccgtt ccagcttcgc
    46261 tgtccacgct tcctggggta cccatgccga ttccgataaa gtcttcggct ttcatattat
    46321 ataaattcaa acgatgattg attgattcaa tgatttcagg aacgatatgc ttaccatcat
    46381 ccaaaatatt ggtatcgatg ctccattttt gctgaatctc tccttctggt gttaaaattg
    46441 caaattttgc tgttgttcca cctaaatcaa tcccaataat cttcttgtcc attacaaatt
    46501 tcctttcttt cggctttctt caattcggtg ctctcttttt aatatgcgcc acgctgtcat
    46561 gtatgtatca tgatcaatca aacggctatc atacagatta cgcaattcga tagtcattaa
    46621 ttcaatgtca tagatgcgtg cacccacata gataaagatg ccaaattttt tgaacagctg
    46681 ctgtacatca tataagctct ccatctttaa gcacttcctt catattatac aagtaaacca
    46741 tattttttta aacccagtaa aatacatatt acaaaaagaa atacaaaaac tagtgtagaa
    46801 agaatccttt ggtgtatcga atagcttgtc tgttgtttcg gtagacccaa aacattccct
    46861 aacactaatc cgccaattag cccaccgaca tgtccccaaa catctacaga ggtatccaga
    46921 agaccaaaaa caaatgtcaa cacaataaat aaaagaaaat gtctgactag ctgctggatc
    46981 gctgggttat gtttaaaatg aaaccctaag ataaacaacg caccgaacaa accgaatatc
    47041 gaagtacttg caccaccaga cagaacattc gcttcgttaa aagcaaagct tgctgtattt
    47101 cccattatcc cgctgaacat ataaatcaag aaaaaacgcc aatggccata gattgcctcg
    47161 atttgctgcc ccataaaata cagtatcagt gagttcatta cgaaatgcat cagtccaaaa
    47221 tggatgaaga ttggtgtcac taaacgccac cattcattga agtgaacaag ataaggacca
    47281 tacattccgg cataataagg gatctcaagt cgcggaaata attccatcat gaggaagacc
    47341 gctgtctgta tccctaaaaa agcataggtc cataacggtc gtcttaacca tgcacgcaat
    47401 tttatttgct gttgataatt catgaaaacc tcccatttca ttttagtcaa taaacaattg
    47461 ttggatcttc tgatcaaagc tctccggttg ccattgttca ttcaattgtt cacgaaaaac
    47521 aaggctacat gtttttttat gaaaattatt taaataccgg tcgtaataac caccgccaaa
    47581 accgattcga taaccggctc tattgtagat aatacctggt acaatgagga gatcgatcat
    47641 ctccggcaaa tagagcttct ctgattgtgg ctcaagaaca ccaaaactcg aacgtacaag
    47701 aacggactgt tcatccattt cataaaacgc catctgacgt ttcggcaaag ttttaggtac
    47761 aactattttc ttttgttctt tttctgctcg ttggataacc ggctgtgtat ccagttcaaa
    47821 ttcattcgat aaagtcgtcc ctatcacttt tgctgacttc cagtactcgc tggcgaatag
    47881 ctgtttatag attcgctgtt ctttttcctt tttcttgcca cttttggcca gatttttcag
    47941 cttttcgatt cctttccggc gtaatcttac tttcaattgg tccaaaaact cacccttcct
    48001 tctctcataa aatggtatag gaaaaaaaga aaaatagcaa tcgaactttc aaataaatgc
    48061 ataaatagaa taaaaagaag acctagaaat gattggcctt ccttttttaa tgaatttcaa
    48121 ctgttcagcg cgtgtttttg tctgataaaa agtgcataca gaaaatatgg cgcaccaaaa
    48181 agggcaatca aaattcctgt aggtacttcc ccacttggta gaatcactct agcaaccata
    48241 tctgcactaa gcagaaaaat ggaaccgaac aaaccgctca gaacaaaggt ccagttattt
    48301 tctttacgta cgactagctt cgcagcatga ggagctagta gtcctacaaa agataaacta
    48361 cctgtcaccg ctacactacc gcttgccaaa gcaacagcta acaacaaaag gattttttga
    48421 cttttagcca cgttaaggcc taatccttgc gcgcgttctt gaccaaatga tagcaataca
    48481 atcctctgtg cataaagtcc tcctagcgga attaagataa ccgcccatgg aagcaatgcc
    48541 caaacataag gccagctcgc accccagatt gatcccgcta accatgcatt cacaaatcca
    48601 taattttcct tagataattg gatcgtgcat agcaatgtta atgcagaaag gccggcatta
    48661 acggcaatac cggacaataa gattcgattc atacccaaaa agccatttcg atcataggcc
    48721 gcaagataga cacagcaagc agctaaaaca ccacctagaa aggctacaac tagcaataga
    48781 aacggcagcg aatagtgcga aaagaaacct aaataaagca tcactaaaaa tcctgctccg
    48841 gcattaatgc ccaatatact agaatctgcc aattcatttc ttgttactcc ttgcaagata
    48901 taaccagaaa cgccaagaca agctcctgct aataaactga tcagtaatcg aggaactcga
    48961 aattctctaa caatcagcgc caaagaagat gattctttcc ctatgaacgt ggcaaaaagc
    49021 tgatccaggt aaataaaagg cgtaccaatc attaaactac ctatagacag gatgacaagt
    49081 acaaaaagca agaaatagaa cgtccaatat atttttttca ccgttgttcc ctccttactt
    49141 gtatcagtaa aaacggaacg ccaatcaatg caatgactaa tccaaaaggt gtttcaaaag
    49201 ggggattgat catcctcgct gtaaaatccg ccaacataac caatccaccg ccaactaaaa
    49261 aagagacagg tagaacataa cgatagtccg ttccaaccat tccgcgaaca actggcggaa
    49321 ccaacagccc aataaatgaa acaggtccga ctaacgaaac ggcaatggct gctaacactg
    49381 caactagaca gaaaacaatc aaccgcgttc gtttaggatg acttccaaga gctaatgcat
    49441 gtgtttcact caaatgaatc aaagatattg cttttcctaa tgaaaaagaa accaaaacag
    49501 caagtatata taaaggaaac aatactgcta attgtttcca tgtgatattg gcagctccgc
    49561 ctacaaacca aaaagcaaga tcctgctgca gattaaagaa ttgggtaagt gcttggctga
    49621 tagcagtaaa aaaagaacta atagctacgc cagctaaaat cagctgtaaa ggattcatgc
    49681 ccattttcga aaactgcaaa aaaccaaata cgagcaaagt tacagcaaaa gaacctgcca
    49741 atgacattag aaacacagaa aaatcagaag ctttaggcca aaaagcaaaa ctaagggcca
    49801 ttgccaacga agcacccgcg ttgattccta gtaagcctga atctgctaaa ggatttcttg
    49861 tcactccttg catcaatgca ccgctcaatg caaaacaact ccctacaaga aatgctcccg
    49921 ccacacgagg caaacgaata tttcgtatga tttggtgact ttgttttgat ctatcaaaat
    49981 caaacaacgc ctgccaaaaa tctgcttttt ccacagtcgc tgcgccattt gaaagagcaa
    50041 caaaaaaaat aatcaaaaga acaaaacaga ccaaaagcag gagtctattt ttcttcatag
    50101 tcctcataat tgatcctcct gctcttttgg caacaaatca taggttagaa aaactggttt
    50161 atttgttctt ggaagcgtga caatcgttgc atcaatctca aatatttttt tcatacactc
    50221 ttttgtgatc acttcttcag gtgtaccttc tgtgatcaac ttaccgtttt tcattgctaa
    50281 taaataatct gaaaaacgtg aagcatgatt aatgtcatga atcgacatca agatcgttgt
    50341 ttgtttcatt tgattgattt cagtcaataa ctgtaggatc tctaattggt gtgccggatc
    50401 taaaaatgtt gtcggttcat ccaagatcaa gatatctgtt tcctgagcaa gcgccatagc
    50461 gatccagaca cgctgggttt gtccaccaga cagcgttgcg atatcccgca tacgaaatcc
    50521 agctagacct gtagcatcca acgcccactc aattgcttca tgatcttgcg tgtccatgcc
    50581 actgaacagt ttttgataag ggtatcgtcc gtaagatact acttcttcta ctgtcatacc
    50641 tgtagggtgt tcactagttt gtgctaataa tgcaattttt tttgcaagtt cttttgaact
    50701 gtatgtagag atatcagagt tattgacaat gatctttcct gatttcacag gtagtatacg
    50761 ggtaaaactt ttcaataaag tagattttcc acttccattt ggcccaatca aactagtgat
    50821 tttcccttca ggaatcgtaa cagacatgtt ttcaatgatt tttttgtttt cgtagccggc
    50881 tgcaatgtca gctgctctta atctcgtcat ttcacccatc ctttcgctga aaatcattct
    50941 caattatgac gctgtttcaa catttcgtca agcgctactt tcatatctat agccaaatga
    51001 agcggttgac agcgttcgaa atatcaggaa taataaccaa tgagaaagat tctcaattat
    51061 gttaggagga aagaaatgaa attaaaaaca aaagtattca cattatctag catattgctg
    51121 ctggctggag tactgagcgc ttgctccccc tcttcagata aagaaacagc tacaactgcc
    51181 aattcgacga cttcatccaa tagtactcat acagtcacag atacacttgg acataaagta
    51241 gagattccaa acgcgcccaa acggatcatc ggcagttact tagaagacta tctgattgca
    51301 ctaggggaaa aacctatcgc ccagtggact gttggcagtg gttccattca agattatctg
    51361 caagatgatt taaaggatgt cccaacgatt tcttatgacc ttccttatga gaaagtgtta
    51421 agctttgagc ctgatttgct gttgatttct tcttccgcta ctgtcgaagg cgggaaatac
    51481 gaacaatata gcaaaatcgc gccaacttat gttgtaaaaa acggaacaga tgtcacatgg
    51541 gaagaacaac taaaagatgt cggcaaagct ttggcaaaag aaaaagaggc agaaaaaatc
    51601 atttccgatt atcaaaaatc catcaagaaa actcgagaag aactagcaga taaaatcgag
    51661 aacaagacag ctgcagtgtt gtgggtgacc aacaactcag cctttatggt cagcaaaaat
    51721 cggtcaagtg gaagaatcgt atacgacgat ctggaatttg gtgttcctga tctagtggaa
    51781 gaggtctcca aaaaagccac ttctgactgg tctgctgttt cctctgaaaa gctagctgaa
    51841 ttagatgctg actacatcat ccttgtgaac agtgacaaag atgctgccat gttcaatgaa
    51901 ccaagctgga agaatctaaa agcagtgaca gacaatcatg tactgcaatt cggacctgaa
    51961 tctagttggc tatataacgg acctattgcc agtcaggaaa tggtcaatga tatcaaagaa
    52021 aaactaaatt gattttaact aaaaaaagca tcttcacgat tagtcgtgag atgctttttt
    52081 tcttattaac aaactagacg catagatttt attcttcttt tgaagcaacc ctatttttta
    52141 ctctttccca tttttgaaaa cgatgggcat atagattttc ttgatccgtt atcccctcat
    52201 actcctctat aagggcgaag tctgaaaaat caatattccc aatatacgta tcgccttcaa
    52261 acgtgctatc gatcaatgtt cgccagatcc gaccagtgta aggaagaagt tcttgaaaaa
    52321 tccgactacc acccgaaaca tagatatctt ctgagatcgt ttgactatac gacaaaagtt
    52381 catcgatcga atgcattact tccgcatttt ctttcgtgcg aaaatttggt tgcgttgtca
    52441 acacgatgat atggcgataa ggaagtgaaa gatcccccat tccttcatag gtctttcttc
    52501 ccatcactaa aattcgatcc attgtatgtt cgcgaaagaa acgcatatca tttggcaatc
    52561 gccatggtaa caaaccattt ttgccgatca gaccattttt atcttgtgcc cacatggaaa
    52621 taaacatatt atccgctcct tatgatttta gatgttttga gataaaaaaa gtctcaattc
    52681 tttgaccagc aaggaattga gaactttaaa aacattattt tgtttcacgg tgcaaagtaa
    52741 ctttgcgttc gcgtgggcaa tatttttgtt tttccaaacg atcaggattg ttacgtttgt
    52801 ttttgcttgt tagatagtta cgttctttac aagaagtaca ctctaaagtg atgtttacgc
    52861 gcatgttttt ccctcccatt tttacaagaa tcctctaaga tgactctcag acttgattat
    52921 cttaccatga ttttttctag attgctagtg tttttcacga ttttgttaag taaaactact
    52981 tgacgataaa tacaacggtt ttacttctca aacaaaatct ttttcttttt gtataaatac
    53041 ccttacactt gtttatatag attatgtttt ttaataaagg gggatactaa tatgccatgg
    53101 aacatgaagg actttcctgc atcaatgaaa aacttagata aattgacaag aaaaaaagca
    53161 atcgacatcg caaatgcatt attagatgaa gggtaccctg ataaccgtgc gatcccaatt
    53221 gccattgatc aagcaaaaaa atggtatgaa gatgctagtg aatcagaaaa acgaactttc
    53281 gaaaaagaaa aaaatccaag caagacggat gaacacgata cgaatcctcg tgctggaaaa
    53341 ctgcttgatt cagatgtgat cgtagaatat gaggaagaac aatggatcgt gaaatctaaa
    53401 ggggcaaaaa aggcaagcaa ccattttgat acaaaaaaag aagcaatcga aaaaggaaag
    53461 caagtagcac aaaacaaaga atcaactttg ttcatttata aaaaagatgg gacaaaagaa
    53521 aaagaa
//
LOCUS       DAZABD010000011        52448 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000011 DAZABD010000000
VERSION     DAZABD010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 52448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52448
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.011"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..793
                     /locus_tag="ACHALG_000728"
                     /pseudo
     CDS             1..793
                     /locus_tag="ACHALG_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
     gene            933..1859
                     /gene="cysK"
                     /locus_tag="ACHALG_000729"
     CDS             933..1859
                     /gene="cysK"
                     /locus_tag="ACHALG_000729"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFN2586161.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2095..2541
                     /locus_tag="ACHALG_000730"
     CDS             2095..2541
                     /locus_tag="ACHALG_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFN2586162.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2813..4165
                     /locus_tag="ACHALG_000731"
     CDS             2813..4165
                     /locus_tag="ACHALG_000731"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HFN2586163.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSDPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQEALGEIETAQ
                     "
     gene            4336..4623
                     /locus_tag="ACHALG_000732"
     CDS             4336..4623
                     /locus_tag="ACHALG_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFN2586164.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            4849..5292
                     /locus_tag="ACHALG_000733"
     CDS             4849..5292
                     /locus_tag="ACHALG_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFN2586165.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(5298..6482)
                     /locus_tag="ACHALG_000734"
     CDS             complement(5298..6482)
                     /locus_tag="ACHALG_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HFN2586166.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            6750..7448
                     /locus_tag="ACHALG_000735"
     CDS             6750..7448
                     /locus_tag="ACHALG_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFN2586167.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            7455..8132
                     /gene="nth"
                     /locus_tag="ACHALG_000736"
     CDS             7455..8132
                     /gene="nth"
                     /locus_tag="ACHALG_000736"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFN2586168.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(8258..8791)
                     /locus_tag="ACHALG_000737"
     CDS             complement(8258..8791)
                     /locus_tag="ACHALG_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2586169.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(9088..11469)
                     /locus_tag="ACHALG_000738"
     CDS             complement(9088..11469)
                     /locus_tag="ACHALG_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFN2586170.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(11459..12106)
                     /gene="recU"
                     /locus_tag="ACHALG_000739"
     CDS             complement(11459..12106)
                     /gene="recU"
                     /locus_tag="ACHALG_000739"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HFN2586171.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            12170..12709
                     /locus_tag="ACHALG_000740"
     CDS             12170..12709
                     /locus_tag="ACHALG_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HFN2586172.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            12799..13218
                     /gene="gpsB"
                     /locus_tag="ACHALG_000741"
     CDS             12799..13218
                     /gene="gpsB"
                     /locus_tag="ACHALG_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HFN2586173.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            13240..13607
                     /gene="rnpB"
                     /locus_tag="ACHALG_000742"
     ncRNA           13240..13607
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHALG_000742"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            13816..14982
                     /locus_tag="ACHALG_000743"
     CDS             13816..14982
                     /locus_tag="ACHALG_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFN2586174.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            15028..16524
                     /locus_tag="ACHALG_000744"
     CDS             15028..16524
                     /locus_tag="ACHALG_000744"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HFN2586175.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     LLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            16605..17288
                     /locus_tag="ACHALG_000745"
     CDS             16605..17288
                     /locus_tag="ACHALG_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFN2586176.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            17933..19123
                     /locus_tag="ACHALG_000746"
     CDS             17933..19123
                     /locus_tag="ACHALG_000746"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFN2586177.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            19225..20178
                     /locus_tag="ACHALG_000747"
     CDS             19225..20178
                     /locus_tag="ACHALG_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFN2586178.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            20247..21596
                     /locus_tag="ACHALG_000748"
     CDS             20247..21596
                     /locus_tag="ACHALG_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFN2586179.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(21779..22102)
                     /locus_tag="ACHALG_000749"
     CDS             complement(21779..22102)
                     /locus_tag="ACHALG_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2586180.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            22231..23394
                     /locus_tag="ACHALG_000750"
     CDS             22231..23394
                     /locus_tag="ACHALG_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFN2586181.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            23464..24813
                     /locus_tag="ACHALG_000751"
     CDS             23464..24813
                     /locus_tag="ACHALG_000751"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFN2586182.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMVFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(24858..25289)
                     /locus_tag="ACHALG_000752"
     CDS             complement(24858..25289)
                     /locus_tag="ACHALG_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN2586183.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            25677..28319
                     /gene="alaS"
                     /locus_tag="ACHALG_000753"
     CDS             25677..28319
                     /gene="alaS"
                     /locus_tag="ACHALG_000753"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFN2586184.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            28519..29115
                     /locus_tag="ACHALG_000754"
     CDS             28519..29115
                     /locus_tag="ACHALG_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFN2586185.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            29237..30385
                     /locus_tag="ACHALG_000755"
     CDS             29237..30385
                     /locus_tag="ACHALG_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFN2586186.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(30423..30836)
                     /locus_tag="ACHALG_000756"
     CDS             complement(30423..30836)
                     /locus_tag="ACHALG_000756"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HFN2586187.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            30915..31346
                     /locus_tag="ACHALG_000757"
     CDS             30915..31346
                     /locus_tag="ACHALG_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HFN2586188.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(31445..31678)
                     /locus_tag="ACHALG_000758"
     CDS             complement(31445..31678)
                     /locus_tag="ACHALG_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFN2586189.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            31861..33531
                     /locus_tag="ACHALG_000759"
     CDS             31861..33531
                     /locus_tag="ACHALG_000759"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFN2586190.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            33678..34064
                     /locus_tag="ACHALG_000760"
     CDS             33678..34064
                     /locus_tag="ACHALG_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2586191.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            34051..34749
                     /locus_tag="ACHALG_000761"
     CDS             34051..34749
                     /locus_tag="ACHALG_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFN2586192.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            34754..35218
                     /gene="lspA"
                     /locus_tag="ACHALG_000762"
     CDS             34754..35218
                     /gene="lspA"
                     /locus_tag="ACHALG_000762"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFN2586193.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            35215..36126
                     /locus_tag="ACHALG_000763"
     CDS             35215..36126
                     /locus_tag="ACHALG_000763"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFN2586194.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            36361..36900
                     /gene="pyrR"
                     /locus_tag="ACHALG_000764"
     CDS             36361..36900
                     /gene="pyrR"
                     /locus_tag="ACHALG_000764"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HFN2586195.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            36914..38197
                     /locus_tag="ACHALG_000765"
     CDS             36914..38197
                     /locus_tag="ACHALG_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFN2586196.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            38210..39136
                     /locus_tag="ACHALG_000766"
     CDS             38210..39136
                     /locus_tag="ACHALG_000766"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HFN2586197.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            39155..40435
                     /locus_tag="ACHALG_000767"
     CDS             39155..40435
                     /locus_tag="ACHALG_000767"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFN2586198.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            40440..41516
                     /locus_tag="ACHALG_000768"
     CDS             40440..41516
                     /locus_tag="ACHALG_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HFN2586199.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            41521..44706
                     /gene="carB"
                     /locus_tag="ACHALG_000769"
     CDS             41521..44706
                     /gene="carB"
                     /locus_tag="ACHALG_000769"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFN2586200.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            44760..45536
                     /locus_tag="ACHALG_000770"
     CDS             44760..45536
                     /locus_tag="ACHALG_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HFN2586201.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            45533..46465
                     /locus_tag="ACHALG_000771"
     CDS             45533..46465
                     /locus_tag="ACHALG_000771"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HFN2586202.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            46462..47178
                     /gene="pyrF"
                     /locus_tag="ACHALG_000772"
     CDS             46462..47178
                     /gene="pyrF"
                     /locus_tag="ACHALG_000772"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFN2586203.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            47185..47814
                     /gene="pyrE"
                     /locus_tag="ACHALG_000773"
     CDS             47185..47814
                     /gene="pyrE"
                     /locus_tag="ACHALG_000773"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFN2586204.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            47802..48116
                     /locus_tag="ACHALG_000774"
     CDS             47802..48116
                     /locus_tag="ACHALG_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2586205.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            48154..48795
                     /locus_tag="ACHALG_000775"
     CDS             48154..48795
                     /locus_tag="ACHALG_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbR-like 5'-deoxynucleotidase"
                     /protein_id="HFN2586206.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            48838..49530
                     /locus_tag="ACHALG_000776"
     CDS             48838..49530
                     /locus_tag="ACHALG_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HFN2586207.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            49527..50429
                     /locus_tag="ACHALG_000777"
     CDS             49527..50429
                     /locus_tag="ACHALG_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFN2586208.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(50587..52293)
                     /gene="efbA"
                     /locus_tag="ACHALG_000778"
     CDS             complement(50587..52293)
                     /gene="efbA"
                     /locus_tag="ACHALG_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HFN2586209.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
BASE COUNT        17146 a         9140 c        11267 g        14895 t
ORIGIN      
        1 gaacgagtct tttagttggg ccgatgatgg ttacagcggt accgcaacat gttcatgcag
       61 atgaacaagg atctcaaaca gttgctgatc ctcaagcgtt tatcgatcaa atcggctggt
      121 ctgcgtcaga agttgctgca agtaatgact tgtatgcttc agtcatgatc gctcaagcgt
      181 tgttggaaag tgcatatggc acttccggat tagctagtgc gccaaattat aatctattcg
      241 gtgtgaaagg aagctataac ggacaggtcg tttatatggc aaccaaagaa tatgtaacgg
      301 acaatgggta acgatgaatg aaccattccg aaaatattct tcttattggg aatccttcca
      361 agaccatgcg aatgttcttc gttcaaccag tttttccacg ggcgattatc attatgctgg
      421 cgtgtggaaa agcaatacaa caagttttta tgatgcaaca gcgtacttga caggtcgtta
      481 tgctaccgat ccaaattatg gggccaaatt gaactcattg attaatactt ataatttaac
      541 ccgttttgat acgccatcaa caggttcggc taccacaagt acaacaacaa cgactactac
      601 gacgacttca acgtcatcat cttcaacaac agatgtaaat acaaataatg gtcagacata
      661 tacagtcgtg tcaggtgata cgctatggga tattgcacaa aaatttggca tgtctgttga
      721 tgatctaatg gcgaaaaatg gcatgtctat gtccagttat tctttattag ttggtcaaga
      781 actaaacgtt tgatttagat aaaaaggcaa agaaactacc atatatggta tatctttgtc
      841 ttttttttgc tagtcaaaga cgaatcactc gccataaatg aacagattgt attatgataa
      901 gtaagaagag caaagacaag gaggaattga agatgacagc tatctataac tcagtcaccg
      961 aattgattgg caaaacgcca atcgtcaaat taaataaaat cgtaccagaa gattcagcag
     1021 atgtttttgt aaagcttgag ttttttaatc caggaggaag tgtaaaagat cgtattgctt
     1081 taagtatgat agaaaaagca gaacacgatg gtctactgaa accaggtgat actatcattg
     1141 aacctacttc tggaaataca ggaatcggat tgtcgatggt aggagttgca aaaggatata
     1201 aagtaatcat cgtgatgcct gagacgatga gtatcgaaag acgtttattg atgaaagggt
     1261 acggcgcaga gttgattttg actcctggtg cggatggtat ttctggatca atcagagaag
     1321 cagaacgttt ggcaaaagaa aacggctact ttttaccatt acaatttgaa aatgaagcaa
     1381 atcctcttgt gcatgagaag acgactggtc ctgagataca tcaagcattt ggagtaaatg
     1441 gcttggacgc gtttgttgca ggtatcggta caggaggaac gatcactggc gctggccgag
     1501 aattaaaacg tgtgtatccg aaaatcgaat taatcggggt agaaccagca gaatctgcta
     1561 ttttagaagg gaaagaagcg ggacctcaca aaatccaagg aatcggtaca ggttttgttc
     1621 ctaaaacact ggatacttct gtgtatgata aagtcctttc tattagtgga gacgaagcga
     1681 tggaaacagc tagagaagtt gggcgaaaag aagggatttt agtaggcatc tcatctggcg
     1741 ctgcaattgc tgccgcgcta aaagtcgcaa aagaattagg aaaaggcaag aaggtattag
     1801 ctgtggttcc cgacaatggt gaacgttatt tatctacagc cctttaccaa gagatataag
     1861 gcacttggtt tttctccaga taggtttatg taaaaaactt ttaaagatga aaagaacaca
     1921 caaaaattta tgatgagaaa aattatttga atagagacgt aaaatagtga acgcgactgt
     1981 tttacgtctt ttttcattaa aatacaataa aattgaattt tcttttcatg ttaagtggat
     2041 tttaaaatgt aaaaatagta tgataagacg agaataacaa cgtggggtgt ttacatggaa
     2101 aataagttag tcgaagaggt tatcgaaaag ttaaaaaaag agaacattcg aatcacacct
     2161 caacggtacg cggttttaga atatttgatt gaacatcatt cccatccaac ggctgatgag
     2221 atttataaag cacttgaaca tcggtttcca aatatgagtg ttgcgactgt ttacaataac
     2281 cttcgcttat ttacttcgat tggatttgtt caagaaatga agtacggcga tgcttctagc
     2341 cgatttgatt tcagttcaaa gcgtcattat catgtaattt gtgaaaactg cggtaagatt
     2401 gttgattttc attacccagg gcttgaagat atagaaatgg ctgcgagcag actgactgat
     2461 tttgagatta atgaacatcg gttggaattg tatggactct gtccggattg taaagagtca
     2521 gcaaaaaagg cgcaaatgta aaaacagttt ctataagtgt tatacctaaa aaaagctagc
     2581 tgtgtaaaaa aacgctagct tttttatttt tttgtctttt attcacaatc ttagatatca
     2641 aagtgaatat tatcacaata agaatcttgt agatattggg ttcttatgct tgaaaaggaa
     2701 tgaaactaca aacaaacctt tgtgaattca caagcattaa actgttgtat ccgttttatt
     2761 tctgttatag tagttttgtc gaaggacaaa atgttaatcg gagggaccat aaatgaaagt
     2821 agtagtaatt ggctgtactc atgcaggtac tgcagctgta aaaagtattt taaaaaatca
     2881 tccagaagca gaagtgacag tatatgaaag aaatgacaat atttctttct tatcttgcgg
     2941 tatcgcactt tatgtcggcg gagtagtaaa ggacccagca ggcttgttct actcaaatcc
     3001 tgaagaatta tcttcattag gagcaacagt taaaatggaa cacgatgtaa cagacgtcga
     3061 tacagaaaat aaaacagtaa cggcaaaaga tctgaaaaca ggagaaacag aaacggtttc
     3121 ttatgataaa ttagtgatga caactggttc atggcctatt atcccaccga ttagtggaat
     3181 cgatgcgaaa aatgtactcc tatgcaaaaa ctacaatcaa gcaaatgaaa ttattgcaca
     3241 agcgaaagaa gcaaaacgtg tagtaattgt tggtggtgga tatatcggta ttgaattagt
     3301 agaagctttt gtcgaatctg gaaaacaagt gacattgatt gatggattag atcgcatctt
     3361 gaataaatat ctagataaac cattcacaga tatcttagaa aaagaattga cagaccgcgg
     3421 tgttactctt gcgctaggcg aaaatgttac tgaatttatc acagatgaag aaggaaaagt
     3481 gaaacaagta gctacaccaa cagatacatt tgatacagat atggtcattt tatgtgtggg
     3541 cttccgtcca aatacaaaac tgatcgaagg aaaagtagat acactgccaa acggggcaat
     3601 caaagtgaat gaatacatgc agacaagtga tccagacatt tttgcagctg gagactctgc
     3661 agtagtcaac tacaatccaa gtggcacgca aaactatatt cctttggcaa caaatgccgt
     3721 tcgtcaaggt ttacttgtgg gaaataactt gacagatcat aaaatggcgt atcgtggtac
     3781 tcaaggaact tcaggacttt acctatttgg ctggacgatc ggttcgacag gtgtgaccaa
     3841 agaaagcgca actttgaacg atctagaagt ccaagcgact gtatttgaag ataattatcg
     3901 tccagagttc atgccaacta ctgaaaaagt aatgatggag ctagtctatg aaaaaggaac
     3961 gaatcgtatc gtaggtgctc agttcatgtc caaatacgac attacacaat cagcaaatac
     4021 catgtcttta gctgttcaaa ataaaatgac tgtagaagat ctcgcacttt cagatttctt
     4081 cttccaacca cattttgacc gcccatggaa ttacttgaac ctacttgcac aagaagcgct
     4141 aggcgaaatc gaaactgcac aataaagaaa aagggttgtg acacaatttt gtcacgaccc
     4201 tctgtctttt tttgtgaaaa gtacaattag ttgtctttct tcgaattttt tgtcacaata
     4261 agcagcaaag ataaagctgt tacataaaag aacaagcagc tttatttgat tttgaaaaga
     4321 aaggtgagaa agaagatgaa taaagaatat tttgtccaac aagatgcatt tttagaagat
     4381 tataaaagtg cttcgaagaa attaacgatc aatattgatg agttggcaga tcaagtactc
     4441 tctcagttga atgaaacgca agaggaatat tacacgatgc cagcaaatga cgccaaagat
     4501 aatgtttctc atcgttttcc tttcagtaga cgcatctact cagatgaaaa tgacggcaca
     4561 ttgaatacag agtacacata tgaaggtcag ccatatgtgc tagaaactga tgatgatctt
     4621 taaagacagt ttttgaaaag gccgctcatc cttaagtgat gacaaaaaat catcacaatt
     4681 accgatgact gtctctttac tatcttttaa tcagaaaaag gggttatgac aagctttgtc
     4741 aacccctttt tttcttggtt tcagattggg ttttcctaca aaatattttt atctttgttg
     4801 aataggaaac aaaaaaacaa tataatgaaa gaaaaagaag gtgtagaaat ggataaaaga
     4861 caactgctaa tagaagaata tctggattta gctaatgaga ttattcatcg taataaacca
     4921 gaaatggaaa aagaatttgc aggatttact ctaaatgaac tagaagtgat tgaacatatc
     4981 gggaaaatcc ctgatcctaa cgtgacaaaa ttagcgaatg ctagttacat gactagagga
     5041 gccatcagta agctaacaaa aaaactgata gctagaggga tcataaacac ttatcaaagc
     5101 gaagagaata aaaaagaaat ctattttcaa ttgacgccaa tcggacaaaa aataaggaat
     5161 agacacgagc aactccatca aaaatgggta cagcgagatg ctactgtatt tgaaaatatg
     5221 tcaaaagaag aatttgacac agtatttcgt tttatcggtc gatacagaga atttatacga
     5281 agtagcaaat gaagctacta ttttttattg tagtgataat aggcaaaaat cataagcaaa
     5341 aatccgaata ccgtcggaag aaatggtgct gcagcataaa gctgtcctgc gattagcggg
     5401 ccgatgacgc gcgatagaga ttgtatactt tgggtaccgc ccattacaag accttgctgg
     5461 ttatctgaga cagaattgga tagttggcca ttgaatacag gagtaaaaat agaatcaccg
     5521 aaaccataac agataatccc aaataaaaac aaaatcggca gtagaagaat tccagataat
     5581 gtgatgaata gataggcgag gatttcactg aacatcccta ttcttgtgat ctgctgttct
     5641 gaaaattttt tgagcaatcg aggcataatg aacaattgcg agacagtatc caagattcca
     5701 atcaaagaga agcttaaacc aaccaaccca gccttccact gaaaggcgtc aatcgaaaat
     5761 tgagaaaaga tagattgaag agagccagct gccaaccaaa cagcaaaacc tgctgtcaat
     5821 aaaggaataa cggatgaaat cttgaataat tgttgtaatt ggtgaaaagg tcttacatgg
     5881 gagaaactta aatctactga acgttttttt attggaaggc tttctagcat aaatgtgtag
     5941 ccataaacgg catttaagaa agtaaagagt gctccaataa atataggaac agaattcccc
     6001 aactcagcta atagaccccc tataatagga cccagcgttg ttcctatacc gactaaagca
     6061 cccatccatc caaaaacttt ggttctttca ttaggttccg tgatgtcggc aaagtaagca
     6121 tataaggtgg agatttctcc tgccgtcaat ccttcaatga tacgaccaag gaacaacatc
     6181 catatcgaat ttcctaaacc aaaaagcaaa tatccgatac ttgatcctaa taaactgata
     6241 ataaggatcg gatgacgccc aaaatgatca cttaagctac cgagaatggg ggcagataag
     6301 aaggcagcta gagcataaac agacatgagc aaagtgatat agaaagcttg ctgatgtatg
     6361 gagtgagaat aaggcagcgt taaaaacgga agtacaggac tgacgatagt aaatcctaat
     6421 ccagtgaata agacagagac gtatccaaaa actttagaat gtttatttgt gaaactgtac
     6481 atgtaaaaac tcctttatgc aataatgtta acttggaaac aaaaatagtg tatagtaatt
     6541 ttgtttcctt gtcaacattc taaggaacag cgaaatctac ttaatttatt tattccgaaa
     6601 agtgaagata cgcatcaaaa acatgaacaa acaaaaatca agtagacgac attgtaaatc
     6661 tgaccgatca gtgaagtaaa tagaaaagaa acctgttctt gcttcccatg ttttttttac
     6721 gtatactgtg attgagcgga ggagaaatca tgttgaattt agaaaattac ttaagcgctg
     6781 gacaaacagt catatctaat cttttactta aaaattatca taggatcggt ctacaatctg
     6841 aagaattttt atttgttcta cagttgcata tggcacagct tgaaggagat actttccctg
     6901 atttgcagat gattgctatg gatatgggaa tcaaacaaga tcagattttt caaatactgg
     6961 atcgtctcgt aacaaatgga tttatcaaga tagaaacgac gattgtcaac ggtaaaaaag
     7021 cagatcgata taatctttac cccatctatg atctattagg tgagtattta aaaacacaag
     7081 aaaaaaaata cgaagaaaaa acacaagaaa aagaaatcca gtcagtgtat caattatttg
     7141 aacaagaatt tggacgcccg ctttcttcga tcgagttcca gcggatcgga caatggctag
     7201 aagaagacca ttatcagccg gaaattttga agctagcact gcgggaagcc gtattaaatc
     7261 aggcttatag ttttaattac gttgaccgca ttttgctatc gtgggaaaga aaaaatctac
     7321 gtacaaaaca acaagtggaa gaagaccaaa aacgaagaaa acagcagctt cttcaaagag
     7381 aagtagaaca gtcagctcaa caagaagaat tgccgaaagt ctcactgaaa aattggttgg
     7441 aggagtagcg gaatatgtta agcaaacaaa aaacaatgga agctctagaa acgatgtatg
     7501 gaatgtttcc cgaagcacat ggagaactca agcacaataa tccatttgag ttattgattg
     7561 cagtgatctt aagtgcccag gcgacagatg tatcagtgaa taaagcaacg ccagatttgt
     7621 ttgcctcttt tccgacaccc gatgcgttag cagaagcttc aatcgatgaa atcattctta
     7681 aaataaaaac gattggtttg tatcgaaata aagcaaaaaa catcaaagcg tgtgcacagc
     7741 agctgattga acgtttcgat gggcaagtgc caacgtcaag agaagaattg atgtctcttc
     7801 ctggtgtagg cagaaagaca gcaaatgtcg tattggggga tgcattcggg ataccagcaa
     7861 tcgcagtaga tacccacgtg gaacgtgtct caaaacgcct gaggatctgc aaactagatg
     7921 ctacggtcat ggaagtagaa gaaacactga tgcggaaagt accacaagaa ttatgggtaa
     7981 agacacatca tacactgatt ttttttggta gatatcattg cacggcaaga aatcccaagt
     8041 gcgaggtatg tcctttgcta tcaatatgtc aagatgggaa aaatcggatg cgtctaaaag
     8101 aaaaggcgct aaaaaagaaa ccacgcctgt agacgtggtt tctttaattg caaataaatg
     8161 tatgaattat cttgttatta tggagaaaag ctgtttcgtt tatctttttt gcatgaaaaa
     8221 gtaaatgtac atactcagta gctaggatgg taacttatta caatttattg cgataaaaat
     8281 aaaagttgtt atttttattg ggaaaatacc cgatgtagaa aataatgcct tccaatatac
     8341 ctataaaata agtccagtat tccaaaggat tatataaata agtgacgtat ctatctaaag
     8401 gtgtaataaa aatgataagt gataataaaa aaataccatt tataactaaa cataaactaa
     8461 tggatttggg cggtttcagg ctcatcagaa aaatatagat tctggtgaac aacaaccata
     8521 aaaaagtgag aatcattgca tgagagcttc tttccataaa ttttccttta agagtagtgc
     8581 ttaaaagtac agaaaaaata ataaatgcaa ggataggagc aattaaagta aatttattta
     8641 ataataaaca aaatttttca gcttttttat tcttggaata acgaggtatt gttttcgata
     8701 aaaaaagatg gtgcagacaa attatgataa ttaagcatac tatcagataa ctcaaaatga
     8761 agccttcttt caaaaaatta ttcaaaacca tatattgtat tttttatttg ataataacaa
     8821 tgggattatt aagaagtggc actaaataaa agactagtgt gtatttagcc gatttaatta
     8881 taaacaagta aatggataaa gtatatgtaa tgataaaaga aaaatggatt ttctaatttt
     8941 taagctaaat gttagatcac acatgtatat gctggatagt cttataaaaa atcatgtaat
     9001 acagcttggt acatgatttt tatttgtagc tgttttttac atagacacaa aaaagaaacc
     9061 acgcttgtag acgtggtttc ttttttacta tctgtttcca gaagctgagg tgctgctgga
     9121 tggtgtatgg tcatttgcag catccgctcc gctagttgct ggctcactgg aagaagcagg
     9181 aggagcagat gaagacggtg tcgagctttc ttcggatgaa gaagaaggaa ccgttgttga
     9241 agacgctggc tcgcttgttt ctgctgtcgt gctttctttt gaggactcag cagttgattc
     9301 gctttgacta gtagagctag agctttgagt cgttgtctct gttgttgagc ttccaggagt
     9361 ctggacatag ctggaagatg gaatagttgt tgatggtgta attgcattcg atctagctgt
     9421 atattgatct ttgtagtaca gttcaccgcc cacacgaatt aatccgcttg gcatctccca
     9481 gtctgtattt gtcacgttag ctgaaacata ctgcataagc tcgcgataaa catcagaggc
     9541 tacgtgtgac gattctgaag tgacaggtgt cattttttta ttgtaacctg tccacacgga
     9601 aatcgaataa tttggcgtat agcccgcaaa taggatatct ggatacaccc cagaagaaat
     9661 accaagtttt gcatactcgt catctgtgta attggatgtt ccggtcttac cggcttgata
     9721 taacccagcg atttgcgcat tcgtaccagt tccttcagtg atcgtatctt ttaaaatgtc
     9781 tgtgatcata taagctgttt ctggagacat ggctgttttt ccatctggct cataagtttc
     9841 ttctgttccg tcttggaaga cgattttatt aacatattga ggtttgtaat aagtccctcc
     9901 attggcgaaa gccgcatagg ctgctgccat ttttaaggaa gaagctccgt attttgtccc
     9961 atcttgttct tccgtattac tagagatcgc attcgattga tggatcgtac tgtattcgat
    10021 cccaagattt tttaagaaag aagccacttt atctgaaccg acttcattga ataatttgac
    10081 tgctggaaca ttacgggata agtaaagtgc ttggcgtaaa gtaatggtgc ccatatattg
    10141 attatcccag tttcctacag gagtcgaagt cccttcatag ttatacggtg cgtcagtaat
    10201 agtttttcca gtagaatatt tcaaatattc aaaagctggt ccgtaatctg tgacaggttt
    10261 catcgtagaa ccaaaatcac gggaagtatt cactgctaag ttgttgccta aagttacgtc
    10321 ttctgcgatg tgacgtccgc caatctgggc ttttactttc ccggtattgg tgtcgataag
    10381 ggtggaggca acttgcatct cgtcgtctgg gtaagagaca tactgatccg tgttgacgat
    10441 atcatatagt ttcttttggg cgtccagatc caaattcgta taaatctcta aaccgtctgt
    10501 gtagacattc ttatctgttt tttcttgtac ttcattgatg acttctttga cataattatc
    10561 gacgatctta gtattgtcat cggattgagt tagttcttgc agaccatccg ttaccggtac
    10621 attaacagct tgatcatatt ctgtttgaga gattttttcg ttttgaagca tcgtatacaa
    10681 aacagtatct cgtcttttct tcgcctgatc tggataaaca taaggatcat aggcggaagg
    10741 ggcttgaggc atcccagcaa gcaatgctgt ttgcggtaaa gaaagttctg ataatttctt
    10801 accaaagtac atctcagaag ctgtctccat cccgtacaag ccgtttgaca tatacacttt
    10861 atttacgtag taggtcaaga tctcttgttt cgattttttt tgttccaatc ttacagccat
    10921 ccacgcttct tgggccttgc gtttcagagt ctgatcctcg gcactggtgg agaagaagga
    10981 aagtttgatc aattgctgtg tcaaagtact tccgccttgc agtccgccag aggtaaagtt
    11041 agagagggca gaaccgatga tccgaatagg gtcaaccccg atgtgtttat aaaaacggcg
    11101 atcttctacc gacacgatcg catcttctaa tgttttcggt aattcattcg cagaaatttt
    11161 ttcacgcttt tctgcgccta aatcttcgaa tagttcgcca tcttgtgtat atagtttgga
    11221 agatacggta gcgtctaatt ttttatccgt caattcgggc gcatcttttg cgtaatacca
    11281 gaaaagtcct acacctgcta aaaagaggat acagaaaaat gagagtattc ctaaaaggat
    11341 cttgataaat aatccttttt tatgtgttcc agaggacttt ccagaacctt tgccagaatt
    11401 cttcttcaca ctctttttag gagtaggctg tttctgtcgt ctagagcttc tagtttgttc
    11461 atttgccatt cttgctttct ccttcaatag ataatgttga taaatagaag tcaaccgcgt
    11521 ccaaataagg aatccgcgga gaaataccag ttttgatctc tattcctagt tcttcgatca
    11581 aagataaagg tagtgatttg cgtccttttt ccttttgggc gtcccagtaa gcaatcaaat
    11641 gaacactatt caaataaaaa cagcgcttca aagacgaaaa ccataacaag acaaagcaga
    11701 ccccattttg ttcgaggcat gctttcatat gttcgatttg atgggcatgg aagtttttaa
    11761 agggaaaaga tgttttgttc ttggtttctt ttgcttcaaa gtccaagtaa aagcctcgat
    11821 agacgccatt atagtctgta gttgaggctt gggtaaagta ggcttccttg atcacagcag
    11881 cacttctctt aggatagtct actttcacga tttggatagg ggtaggcttt ttatggataa
    11941 ccgccaaccc gcgggaaaga taatatttat tactttcatt gattgcttct tcaaagcgca
    12001 tcccccgatt gccgaattca gtgatctgat ttcttttttc ttgaattttt tcgacgggag
    12061 aggcattctt ataataaggt tggccatttg gatagcgcaa taccatcttt aatcacactc
    12121 ctaggccaca ttatagcaaa aaagcggata gaaagaaaag gaatctgaga tggataagtc
    12181 aaaagtaatc tatgtgtctg ggtatcgaag tttcgaactc ggtgttttta aagaaaatga
    12241 tcctaaaatc caagttgtta aaaatgtttt aaaaaaagaa ctccatcagt tagcagaaga
    12301 agggctggaa tgggtacttg tcagtgggaa tttgggcgtc gaactatggg ctgtgcaaat
    12361 agtagcggaa ttgaaagaag aatatccgga actatctctt ggtattttgt atcctttttc
    12421 tgattttggt aataactgga atgaagggaa ccaagaaaaa aaacaattag cagaacaatt
    12481 agcagattat gtcgaagctg ttagccatca accctaccaa tcacctagtc agctaaagaa
    12541 tcacactcgt tttatacttg agcatacagt gggctgtctg ctgatctatg atgaagaatt
    12601 ccctggaaaa acgaagtatt tttttgaaga tgcacagaat taccaagcag atcatcccta
    12661 cgaaattcgg ctgatcagta tggacgatct tcaaaatttt agtgaataat cgatattgtg
    12721 tttgatttat caactttttt cctatacaat gaaaaaggta gaaatgttat tacgtaaatg
    12781 aaaactagag gtgtaaatat ggcgaatttg gtttatagtc ctaaagacat tttacaacaa
    12841 gaattcaaaa caaaaatgcg cggctatgat cccgttgaag ttgatgaatt cttagacaat
    12901 atcatcaaag attatgaaac ttacagcaaa gaactccttg ctttacaaga agaaaacgat
    12961 cgattgagtg caaaagtagc tcaattatct aaaactcaag gagcagctca aacacgcgtg
    13021 caacaaacag aggcaccaaa gagcgcagca gtgaccaatt ttgatatctt aaaacgcttg
    13081 tcaaatcttg aacgtgaagt atttggtaaa aagcttgatc aacaagcaag tgcagtcaaa
    13141 ccagcacaac ctaatccaaa taactacaca aatgcagaca caagcttaga tgataatgaa
    13201 aaaacacgtc aattttaatt tttgattaat gaatttgcgg ttttcgggta atcgcggtct
    13261 gacttgatca ggctgaggaa agtccatgct cgcacaggct gtgatgcctg tagtgttcgt
    13321 gcttagcgaa accataagct aaggtactct ttggagtaac ggcaggaaaa caagctaagg
    13381 cttcggctat gcttaagtat ccttgaaagt gccacagtga cgaagcggct ggggaaaccc
    13441 atccggtgga acgcggtaaa cccctcgagc gagcaaccca aacaatggta ggggcgcttt
    13501 ttgtctggaa gaagaacagg acaaaaaggc agtataactg cagatagatg gttaccgctt
    13561 tgttttttct ccctggaaaa gcaaagtaca gaacatggct tatagaaaac cgcaaacatc
    13621 agggaattag gtcgtgacaa aagtaatgcg ttataagaaa caagaaaaac atttgaaagc
    13681 tatttttcaa tacttcagat gaatcgttct tattacaaaa caaacttttg tatcaccggt
    13741 tattcggaat ttgagtaaaa gtgtggggta ggactgatgt cccacctcct ttttttataa
    13801 ataaggagat tgtgtatgga aaaaaacagg aagttcaacc tcgtcgcaac tgcggcaagc
    13861 gggctagaag cactggttgg taaagaatta agagatcttg gaatcgaatg ccaagttgag
    13921 aacggacgtg cacgttttga aggaacaatg gaaacgatcg ctacagctaa tttatggtta
    13981 cgtacagcag atcgagtaaa aatcgtggtt ggggaattcg atgcgtatac gtttgatgaa
    14041 ttgtttgaaa acgtaaaagc attgccttgg gaagattttc ttccactaga tgcagctttt
    14101 cctgtagctg gaaaatcgat caagtcgaaa ctatatagca cacctgattg ccaagcgatc
    14161 acaaaaaaag caatcgttga acgtctccgt acctattatc accgtccagc ttctgtacca
    14221 ctgacggaaa ctggcgcaca ttttcagttg gaagtagctt tgctgaaaga ccatgtgatg
    14281 gtaactctgg atacgacagg accaagttta ttcaaacgtg gttatcgctt agaaaaaggt
    14341 ggagcaccat tgaaagaaaa catggcagca gcacttatta tgttgacaaa ttggcgaaaa
    14401 gaccgtccat tttatgatcc tgtatgtggt tcaggtacgc tttgtattga agcagcctta
    14461 ataggccata atatcgcacc aggattcaat cgtgagtttg cttgcgaatc ttgggattgg
    14521 ttcagtcaag aaatcatgga aaatgttcga tcagcagccg aagaaaaagc agattatgat
    14581 attgaattag atattactgg ttcagatatc aatggacgga tgattgaaat tgccaaagcc
    14641 aatgcagaag aaatcggatt aggctcttcg attaccttta agcaacaagc agtcaaagac
    14701 ttcaaaacgg ataaagaata cggggtcatc gtggctaatc cgccttacgg agaacgtcta
    14761 ggagaagaag aaactgttcg gaaattatat aaagaaatgg gcgatgtatt ccgtccatta
    14821 aaaacatgga gtaaatatat cttgactagc gacttagcgt ttgaggaatt ttacggacaa
    14881 aaagcaacca aaaaaagaaa attgtacaat ggtgcattgc gtacggatct attccagtat
    14941 tggggaactc gtccgcctag aaaaaaagag aaagacgaat aatcttacta attgattcat
    15001 tcaattggat gtttggagga atagggaatg aaagaaaaag agtttttaca agaagtaaaa
    15061 gaaatcaatc ttctacaaca agctttagga atcctcgatt gggatacaca aacaggtatg
    15121 cctgaaaaag ctagtgcata ccgaagtgaa gtagacagct atctttacag catttatttt
    15181 tcaaaaaaaa tcggtccaaa aatcaaagaa gccattcgct atttttctga acatccggaa
    15241 gaattgtcgg aagttggaac tgccgttttt gccaaagtaa aagaagagta tgatttggaa
    15301 catcgcgtac cggaagatct tatgagaaaa tatacagctg ccgtttcttc agcacacgga
    15361 caatggcaaa aagctagaga aacacaacaa ttttctgatt ttcaacacgc attgaatcaa
    15421 aatattgaat taacaaaaca gttgatacct tactggagaa aagatgaaaa gacgaattat
    15481 gatgttttgc tgaatcaata cgaacccggg atgactgtcg aaatccttga taaggtattt
    15541 gatcagctta aagaaggaat tctttcgatc cgccgtacgc tccaagaaaa agggactgaa
    15601 cctgaaacag actttttaaa tcggagaatg acgaaaaaac aacagaagag atttgtagtg
    15661 aaagtaatcg aacaacttgg ttatgatttt agtcgtggtc gtttagatga cactgtccac
    15721 ccatttatga ctggaattaa tccgaatgat gcacgaatca cgacacgatg gaacgaaaca
    15781 gatttcaaaa tggctgtttt tggagtgatc cacgaggctg gtcatggtat gtatgaacaa
    15841 gacatcgatg ataaatatgc gtatacaccg gtttatgaag gtacatcaat gggtatccat
    15901 gaatcacaat cgttgtttaa cgagatcatt attggaagta gtcgcagctt ctggaaaaag
    15961 caatatcctt tctttcaaga atgtgcagaa ggaacattcg atgatattcc ttttgacaaa
    16021 ttttatcgtt tattgaacca tacaaaatca agtttggttc gtatcgaggc agacagtttg
    16081 acttaccctc tccatatcat cattcgttat gaaatcgaaa aaatgatttt caatgaaggg
    16141 gtagatgttg ctgaactacc tgaaatatgg aatcaaaaat atgaagagta tctgggaata
    16201 cgaccaacaa atgatgtgga aggcatccta caagacgttc attggtcagg tgcaagcttt
    16261 ggttattttc cctcttatgc attagggtac atgtatgcgg cacaattact tcattctttg
    16321 cagcaagaaa tagatgtgga aaatgttcta gcgtcagacg attattctcc aatcaaaaac
    16381 tggatgtcag agcatatcca tcaatatggt gcatctagaa aaccaaatca attaatcatg
    16441 gatgcaacac atgaaccgct taatccgcaa tacctgatcg actatatgaa agacctgtat
    16501 tttaatgtat atcaggtaaa ataaaataca atttacaaag atagctgaaa ttcatctaat
    16561 ctatagatgt tttcagctat ttacttaaga aagaagtgga acgaatggat aaattacaag
    16621 tgatcgctga ttatagtatg gaccaattag cgcatgatca aacaggacat gggagcgatc
    16681 ataccaaaag agttgtcaag ttagctgaaa aaattttgga gacggaaccg caggcagacc
    16741 gctttgtgac gcttgcagca gcttacctac atgatacgat tgatgataaa gtcgttaaaa
    16801 atgagaacga ggcaaagcaa cagcttcgtg tattccttcg ctctttgcca atagtagagg
    16861 aacagatctc gatgattttt tccattatcg aaaatatgtc cttttctaaa aatctctctg
    16921 aagcggccga actatctctg gaaggtaaga tcgttcaaga cgctgatcgt atcgaagcat
    16981 taggagcaat cggcattttg cgtactgctt atttcggcgg tggacatggg cacccgattt
    17041 ttgatcctga actgtatccg caaacattca aggacaaaga aaattaccga aaaggcacga
    17101 cagtgatcaa tcatttttat gaaaaactgt tcttgctgtc tgataaaatg aatactgatt
    17161 acggatatga agaagccaaa cgaagagaaa ccttcatgcg tgaattctta gaagaatttt
    17221 ttctggaatg gtctattgac gatgaaaatt ttactccaga caattggatc aaaaataaca
    17281 aaaaatgatg gaggttgtga cagaagtggt cagctccaag atatttcgga tcattttccg
    17341 taaaagttac ttctggaaca ctgtttacca agaggtcgtg agcctgccgt tcagctccga
    17401 gtcacaagga acaatttcaa aaaatagctt ctcgtgtttg atgaatattg taacttgtga
    17461 ttcggaattg gcaggagaac accgtttatt cggaagaata ggactgtgac aagacttttg
    17521 tcacagtcct tttttcgaat tcaacaagta aaacttttaa gaaatattca gagagtaaaa
    17581 ttaaaaattg ctgataaacg aagatggaaa ttttatgtat ttttattggt ctatgcaata
    17641 aataaattaa atattcagaa tacaattgat tttaacgaaa gggcttctat caatggttgt
    17701 gttgtattta tctgcccagg tagagtaaaa tagatcatta tctaaaaggt aattgttcat
    17761 actatataag tcatttgaaa gtaaaaagat actgtaaata aaaaatgtgg ttaaataatt
    17821 ctcataatca gtgtaagcgt tatcaaaaat atagcatact tgctttgtag atgaatgaca
    17881 cgatatatgt ttggtatgtt ctgcaagaca gatgaatatg gaggtggaga caatgtcttc
    17941 caaagaacta acaatgtatt tagagaaaga attggatcaa ctgaaagaaa aggggttata
    18001 caatacgatc gacatactag aaagtgaaaa tggtgcttgt atcatcgtag acggaaaaaa
    18061 gatgatcaat ctagcatcca acaattatct tggatttgcc aatcgcgaag aattgaaaaa
    18121 agcatgtatt gaagcaaccg aaacttatgg tgtaggagca ggagcagtcc gaacgatcaa
    18181 tggttcgctg aaaatccatc agcaattaga agaaaaaatc gcagaattca aagggacaga
    18241 agcagcaatc gcctttcaat ctggattcaa ctgcaatatg ggcgctatct ctgcagtaat
    18301 gacaaaagaa gatgctatct tatcggatga attaaaccat gcatctatta ttgacggttg
    18361 ccgcttatcc ggagcaaaaa tcatccgaat taaacatcaa gatatgaaag accttgagaa
    18421 aaaagcaaag gaagcgatag aaagtaagaa atataaaaaa atcatgtata tcacagatgg
    18481 tgttttctcc atggatggag acattgcacg attgccggaa attattccga ttgttgaaaa
    18541 atatggactg atcacctacg tcgatgatgc gcacggctca ggcgtaactg gaaaaggtgc
    18601 aggaaccgtc aagcattttg gattaagcga caaaatcgat atgcaaatgg gcacgttatc
    18661 gaaagctata ggtgttgttg ggggttatgt agctggatcg aaaacactaa tcgactggtt
    18721 gaaagctaga agtcggccat tcctattttc tacttcgcta actccaggtg cagctgcagc
    18781 tgcattggct tcgattactt tgatgcaaga acatccggaa cttgtcgaaa aagtttggga
    18841 aaatgctaat tattttaaag aagaattgaa aaaagtaggc tataacatcg gtatgtctga
    18901 aacacctatt actccagtca ttttgggaga cgaaaaagtg actcagactt tctctaagaa
    18961 gctgatcgaa catgggattt atgctaaacc aatcgtttat ccaactgtgc ctcttgggac
    19021 aggcaggatc agaaatatgc cgactgcgga acatacgagg gaaatgcttg atgaagcagt
    19081 agcagtttat caaaaaatag gaaaagaaat ggaaattatt taactaaatt tagttttttc
    19141 gtctatttaa aattagagaa catgttcacg catatattgt taatcatctg aagatagtag
    19201 taaaaaagat cagggaggat catcatgaaa aaagtattgg tgacaggatg tttaggacaa
    19261 attggttcag agttgatcac acggctacgt caagaactag gcagtgaaaa tgtcattgcg
    19321 acagatatac gcatgcctga agtcattgag aaaaacgata tctttgagat cctagatgta
    19381 atggattacg aaaaaatgcg cagcatagca gaacaatatc aagtggacac attgattcat
    19441 ttggctgctc ttttgtcagc tgttgcagaa gcaaaaccac aattagcctg gaatttgaac
    19501 gtgactgggc tggtcaatgc attggaagtc gctagagaat tccagctgaa attttttacg
    19561 ccaagttcaa tcggcgcgtt tggtccaaat actccaaaag atcatacccc acaagatact
    19621 cttcagcgtc ctacaaccat gtatggggta acgaaggtag ctggagaact actatgtgat
    19681 tattattatg tgaaatatgg cgtagatact cgtggtgtcc gtttccctgg attgatttct
    19741 tatgcaacct tgccaggagg cggtacgaca gactacgcag tcgatattta ttattcagca
    19801 atcaaagaag gaaagtacac tagtttcatt gatcaaggta catcaatgga tatgatgtat
    19861 atgccagatg cgatcgatgc cattataaaa ctcatgaatg cagatcctga tcgattggtt
    19921 catcgaaatg ccttcaatat atcggcgatg tcttttgacc cggaagagat caaagcatct
    19981 attcaaaaaa tcataccaga ttttgaaatg gattatgatg tggatcctgt acgtcagtca
    20041 atagcagaat cttggccaaa ttcactagat gtttcttgtg cgcaagagga atggggcttt
    20101 tcgccgaaat acgacttaga tgtgatgaca aaggatatgt tggaaaaact gaaagaaaaa
    20161 ttggtagaga cggtataaaa caagaaccac atccaaaaag ttttttatac ttttgggatt
    20221 ggctatggta tgaaagggga caagggatgg aagaatatca agccacgcca gtaaaagaag
    20281 taaaaataga aaatgaatta aaacgtacga tgggattttt tactgcttta tcgactgtta
    20341 tgggcacagt gatcggtgca ggggtatttt ttaaagccgc aagcgtagca gaagttacag
    20401 gatccgccag tttgcatatg ttttcttggt ttctcggtgg tatgatttct gtctgtgcag
    20461 gactaacagg agctgaactg gcagctgcta ttccagaaac aggagggatg attaaatata
    20521 tcgaacggat ttacggaaat acagcagctt ttttattggg ctgggcgcaa gtcgtgattt
    20581 attttccagc aaatgtagca gccttgtcga tcattttcgg tacacagttc gtcaatctat
    20641 ttggtctttc ccagtcaatg attgttcccg tagctgttac agctgctgta tcgatcctgt
    20701 tgatcaattt tttaggttct aaagctggag gggcttttca gtcgattacg cttgtctgca
    20761 agcttattcc tttgtttgtg atcgtcattt tcggtttgtt ccgacaagaa ggagtagact
    20821 tccaactgtt tccgatccaa gcaggagaaa atctttcatt tttctcagcc ttgggagcgg
    20881 gcttgctagc aacgatgttc gcatacgacg gttggataca cgtaggaaat atttcgggag
    20941 aattgaaaaa gccggcgaaa gatttgccaa aagcaatttc tttaggaatc atcggaatca
    21001 tgatcgttta tttattagta aatgcggtgt tcttaagaac cgcatctatc gacggagtcg
    21061 caggaaacag caatgcggca agtgacgttg caaaaatgat ttttggcggc ttcggtggaa
    21121 gactggtcac agttggtatc ttgatttctg tttatggaac catcaatggc tataccctta
    21181 ctggaatgcg tctcccttat gttatggcaa aagaaaacaa cctgcctttt agcaagctct
    21241 ttgctaaact tcatgataaa actaaagttc ctgtagccgc aggtatctta gagctagtca
    21301 tcgctattgg catgatgatg atcggtggtt ttgatacact aacggatatg ctgatctttg
    21361 tcatttggat tttttatacg atggtctttg ttggtgtcat tcttcttcgt aaaaaagaac
    21421 ctgatttgtt tcgaccatac aaagttccaa tgtatccgtt tattccacta gtagcaatca
    21481 ttggagggac ttttatcgta tcaagcacgc ttatcactca gaccttttta gcttcgatgg
    21541 ggatagtatt gacacttgct ggtatcccga tttatctcta tttaaaacgt agatagctaa
    21601 aacattcgaa ttttattaga actgcacttc tctgccaaac aaaatatttt atttaaatga
    21661 ggagtacgtg gaagaaatgt caaaagtact cgttaatttt gatcacgtaa atgtcgtttt
    21721 gtctacgatg aaatagcaaa gtaaaagaca gaaatcgggc tcaaccgatt tctgtctttt
    21781 actttgattt gcgtaactga actattcggc gtacgccgcg ataaatattt aataaaacca
    21841 agcaaccaga aatgaaaatg ggaagccaaa tcagatagac agggtagcca agcactttag
    21901 acaaagcatg tggagtcatt ttatccattt caagaagtgc ttgctgccaa gtctgaagat
    21961 tgaagactag catgaaaccc gaaataatca ggagaataaa tcctaaataa tgaattgtag
    22021 ttctttttct tacataagcg atcgtagcat aaaggaacag taaaaaaata ggtacgccaa
    22081 aaacaaagta catattcagc atctcgacac cccctttgtg tttatcttac tttactttga
    22141 aagaaatgag aaataatagc ctcgttactt gcttgaaaca gcaaaggact gctaagataa
    22201 gagagataca aagaatcaag gaggagtcat atggcaagtt atatcgaacc gattcgtttt
    22261 gcgctgattg tttttccatt tttagcctta gcgatctcat ctgttttttt tatttatgaa
    22321 tatcgaaagt atggaacatt tctagtaaca agggcagtta ttttgtattc tttcgttttt
    22381 tatttattgt gtgcgtattt tttggtgatc ttaccacttc cggcaaagga gacggttgcc
    22441 caaatgacag ggccaaagat ggagctgcat ttatttgctt catggcaaca tttcagagac
    22501 aacacggtat tggtcttgtc tgaacctagt acctacctgc cggcaatgaa gcagagtgta
    22561 tttttggaac cagcttttaa tgttttatta ctcataccat ttggcattta tttaagatat
    22621 tatttccggc tttcttttat gaaaacgatg attgctagtt tctgcctttc tctatttttt
    22681 gaattgacgc agctaagtgg actttatttt atctacccaa gaccataccg cttatttgat
    22741 gtgaatgatt tagtgacaaa tacattgggt ggaatcattg gttttgtcgt tacgccgtta
    22801 tttacattta tgcttcctac aagaagacga atggatgaac tttcttatga aaaaggaaag
    22861 actgtctcat tgactcgacg cttggtcgct tatttgatcg actggacatt tttatctctt
    22921 attaatttgg tagcggcgat tttctttaaa attgcgttcg attcaggggc agttacttct
    22981 catatctggt ggcttttatt agaagtaggg gtatacttta tgattgtacc gtttataacc
    23041 aatggatgga cacttggcaa aaaaattgtg cgtatccgtt tggtggaaga agaccgcgaa
    23101 cgaatttctt taaaagcatt gatgatccgc tacgggtatc tgtactttct tttttatggt
    23161 ttaagctggt tggggcagtt gcttcagcaa atcatgaatt cgacaacgaa tacggtgttg
    23221 cagatgagtg cagtattcat ttctctcggt gtcttgttac tacaaggtat ttttatttta
    23281 aatatcttgc tatcattttt gagaaaaaaa cggattctgt tttatgaaaa agctagtcac
    23341 acatatactg ttagcacgat tggccaaaca aaggatgatg attctcgaaa ttagctgagc
    23401 aaaaacgctt tgtctgtgtt ataatgccaa agtcgtgata atttaaagag aagaggtgca
    23461 cgaatgaata attttgatat attccgcttc aaaccgttta tcaatgaagc gttaaccgca
    23521 aagggcttca aacagcccac agaggtccag gaacgtttga ttcctttaat tgcaaaagga
    23581 aaaaatatca ttgggcaatc tcaaactggt acaggaaaaa cacatacgtt tttgttgcct
    23641 ttgtttgaaa aaatcaatgc agaaaaagag gaagtacaag ttgttattac agctccaagt
    23701 agggaactag ctgcacagat ctatgaagca gctacacaaa tcgctaaatt ttctacaccg
    23761 gagatccgtg tctcgaattt tgtcggcggt acagacaaac agcgtcaatt aaatcgtctg
    23821 catcaccaac agccacaggt tgtagtaggc acgcctggta gaatattaga catgatgaat
    23881 gaacaagcgc tgaagattca caccgcattt gcatttgttg tcgatgaagc ggatatgaca
    23941 ttggatatgg tttttttaaa tgaagtagac caaattgcaa gtcgattacc agaaaagctg
    24001 cagtttttag ttttttctgc cactattcca gaaaaacttc gtccattctt acgaaaatac
    24061 atggaaaatc caatcattga agaaatcaaa ccaaaagcag ttatttctga agacattgaa
    24121 aattggctga tttcaacaaa aggtaaagat gagaatcaaa tgatttatca actgctgaca
    24181 attggtcatc cttatttagc aatcgtattt gccaatacaa aacagcatgt ggatgaaatt
    24241 gccgattact tgaaaaacca aggcttgaaa gtagcaaaga ttcatggaga catcacccca
    24301 agagagagaa aacgagtgat gcgacaagta cagaatctgg agtatcaata tgttgtagcg
    24361 actgatttag ctgctagagg gatcgatatc gaaggtgtct ctcatgttat taatgcagaa
    24421 attccagaag atctagactt ctttatccat cgtgtcggac gtactggacg taatggctta
    24481 aaaggaacgg ccattacgtt atattcgcca aatgatgagc aagccattga tgcgatagaa
    24541 aaattgggcg tctcatttga accaaaagaa atcaaaaacg gggaaatcgt caaaacatac
    24601 gatcgtaata gacgtgccaa acgagaaaaa tcaaaagatg tattagatcc cacgctgatt
    24661 ggtcttgtga agaagaaaaa gaaaaagatc aagcctggtt ataagaaaaa aatcgattgg
    24721 gcaattgcag aaaaaaataa aaaagaacgc aaaatcgaac gcagacagca aagccgtact
    24781 gctcgtaaaa ataagaaaaa ttcacataaa taaaaaacaa gaggttgtga caaaaggcgt
    24841 gtcacaatct cttgttttta agctagactt tttttcattc cgatacaaag tacttcatct
    24901 tcaataaaag gttctgtgca agtatgatac ccttgttttt cataaaaatc tacagccgtc
    24961 atttcagcta ctacataact agtctgacaa ccttctgaaa ccgcccgttt ttcagcagct
    25021 tcaagcaact gaccgcctaa cccttttcct cggtattctt ttgctacgca aaatcgttcc
    25081 ggcttgaagc aagtaggtgt tttcttttca taacgcaaag tagctactgg tcttttttga
    25141 tcaagaaata gataataggt ttgttgcggg atatcttttc cgtcgaattc tagttctgca
    25201 ggaatccctt gttctaatac aaaaacttga tatctcaaaa agcagctagc tgcgcgtatc
    25261 cattcatccg ctccaactat ttttttcatc atattcctcc ttgtcatgaa tagcagagat
    25321 gtattgattc ctttattctt atcgtaccaa aaatcgtgga gtatgaccaa aaggaaatga
    25381 aaataaaaat tgagatattg acagtgatca atagaatgcg tatactaatt aagtacgaaa
    25441 aggatatgat ttgtttttta agactcacag aaaattcttt cttggctgaa aaaagaatag
    25501 tcactaatca aattgctccc tttttctatt ttggtagaaa taccgacgca gctcagcgtt
    25561 atcgagttta agaggtaatg attacacatc attgcaatca aggtggtacc gcgagttttc
    25621 gtccttggct gtgatggtgt ttttttgtat ttaatttata gaaagcaggt aatgacatga
    25681 aacaattaag tagtgcacaa gttcgacaaa tgtttcttga tttcttccaa tctaaaggac
    25741 atacgattga acctagtgcg tctttgatcc ccgtgaacga tccaacctta ttgtggatca
    25801 actctggtgt tgcaacattg aaaaaatatt ttgatggttc agttgtgcca gaaaatccaa
    25861 gaatcacaaa tgcccaaaaa tctattcgaa caaatgatat cgaaaatgtc ggtaaaacag
    25921 cacggcacca tacgatgttt gagatgctgg gtaatttttc gattggtgac tatttcaaaa
    25981 atgaagccat ccactgggca tgggaatttt tgactgatcc aaaatggatg gcattcgatc
    26041 cagaaaaact ttatgtaaca gtttatccaa aagatacaga agcaaaacgc atctggcatg
    26101 aagaagttgg cctgccacta gatcacatta ttgatgtaga agacaacttc tgggatatcg
    26161 gtgcaggccc atgtggtccg gatacggaaa ttttctatga ccgtggagaa gcttataacg
    26221 atttagcaga agacgatcct gaaaactatc caggaggaga aaatgaacgt tatttagaaa
    26281 tctggaactt agtattttca gaattcaacc ataagcctga taacacattt gaaccacttc
    26341 ctcataaaaa cattgatacg gggatgggat tggaacgcat ggtctcaatc gttcaagatg
    26401 cgtcaacgaa ctttgagaca gacttgttta tgcctattat ccacgccgta gaaaagctaa
    26461 gcaaacaagt aaaatatggt gaaaacccag taacagatat ttcttacaaa gtaatcgcag
    26521 atcatattcg cgccctttca tttgctattg gcgacggtgc attgccatcg aatgaaggga
    26581 gagggtatgt cttacgccgt ttgcttcgtc gtgctgtcat gcatgggaaa aaattaggta
    26641 ttgaacaagc attcttatat cgtttagtcc ctgttgttgg ggaaatcatg gtaagttatt
    26701 atcctgaagt acttcaacaa aaagaattta ttgaaaaagt tatccgtaca gaagaagaac
    26761 gtttcaatga aacaatcaat gaaggtttgg aaatcttaaa tcaagtgatc caagaagtaa
    26821 aaaacgaaaa cgggacgact ctagcaggaa aagacatctt caaactatac gacacatatg
    26881 gcttcccggt agaattaacg gaagaggtgg cagaagatga aggactgaaa gttgatcatg
    26941 ctggttttga aaaagaaatg gatgcacaac gcgaacgtgc tcgctctgcc cgcagtactg
    27001 aaaaatcaat gggggtacaa tctgctttat tgacagatat caaagtagaa agtaaattca
    27061 ttggatacac agaacaagaa tcagatagtc aattattggt catcgttaaa ggtgagtctc
    27121 ttttgaatga agtcgcagaa ggtgaagctc aaatgatctt tgatcaaaca cctttctatg
    27181 cagaaatggg tgggcaagta gcggatcaag gatggatcat gaatgaagta ggcgaaatcg
    27241 tagcagttgt tacagatgtt caaaaagcgc ctaatggtca atttatgcac accgtagaag
    27301 taaaagcacc aatgagtgaa ggacaaacct atcatctatt gatcgatcaa tcgatgcgca
    27361 atcggatcat caaaaaccat acagcaaccc atctactgca caaggcattg aaagaagtac
    27421 ttggcgagca tgcaaatcaa gctggttctt tagtcgctcc tggacattta cgattcgact
    27481 ttacccactt tggtcaaatc acttcagatg aattagctga aatggaaaga atcgtcaacg
    27541 aaaaaatctg ggaagctatc ccagttgaaa caattgaaac agatatcgat acagccaaaa
    27601 acatgggtgc aatggctttg tttggtgaaa aatacggaca tgacgttcga gtagtaaata
    27661 ttggcggctg gtcaatcgaa ctgtgcggag gcacacatgt agcaaataca gaaaacatcg
    27721 gaatcttcaa aatcgtttct gaatcaggaa ttggtgcagg cgtgcgacga atcgaggctg
    27781 taacaagtaa agaagcgtac gaactacttg aagaagaaga aaaacaacta aaacaagtag
    27841 caacaattgt taaatcacct caattgaaag aagttgtttc aaaagcggaa caattgcaac
    27901 agcaagtaag agagctacaa aaggaaaatg aacaattagc tagcaagtta gcgaatcaac
    27961 aagctggtga tgtattcaaa gatccagaaa cgatcaatgg aaaaacaatc attgcagcac
    28021 aagtaaaagt gaaagatatg aatcagctgc gtcaattagc tgaccaatgg aaacaaaaaa
    28081 atatttcaga tattcttgtt ttagcaaacg aacaagaagg aaaagtcagt ctgctagcgg
    28141 caatgacgaa agaatcaaat gaagctggac taaaagctgg cgacttgatt aaagcaatcg
    28201 caccgaaagt aggcggtggc ggtggtggac gtccagacat ggcacaagct ggcgggaaaa
    28261 atcctgctgg tattccagat gcacttaaag ctgcgaaaga atgggttgcg caacaatagg
    28321 gcgtttacgt ttgacagaaa aacaaaagtt caaacattga acgaaacatg attatggggg
    28381 cgaagacaaa caagtcttgc cctcatactt ttttcaattg gatggcgcat ctaatcaaat
    28441 ggtcggattc ggatggtctt tagttgacga aagtgacaga aatttttacg attagtataa
    28501 ataaaaaggg gtgaaaaaat gaatagtaga aataagacct ttcgactagt cttacgtgcg
    28561 atcttgttag caatcatcat cgtacaagcg atggttcctt ggctggggtt tattccatta
    28621 ggattcatta gtttaacaat tatccatatc acagtaatta ttgctgcagt agtattaggc
    28681 ccaaaagatg gcatggtgat tggcttgttt tggggaatcg ctacaattgt tcgtgcctat
    28741 gccatgccaa caacgccatt tgatacatta gtgttcacga atccgattat ctcggtagtt
    28801 cccagagtac ttgttggctt agtagcaggc ttggttttcc actggattta tcaaaaaaat
    28861 aaatcaataa cgatcagctc agcctttgca ggggtactgg gttcattggt caatacagtc
    28921 ttagtgttag gattcatggg attattttat acaggtgcaa cggcagatgc ctatggtgta
    28981 gactctgctc ttttatttaa aaccttagca ggggttgcag caatcaatgg gataccagaa
    29041 gcaataggtg cgggaatcat aacaccatta ctagcaaaag cgttatttgc agcaacaccg
    29101 ttgaaaccag aataatgacc atcttttaat agtaatagga cgcacgatag atagaaaaag
    29161 gaagagagga gaggaaaaca atcgtcctct ccatcttttt acctgagcaa caagcaaact
    29221 agaaggggta aaaaacttgg ctaagaaaca agaagaaaaa aagaaacaaa taccgcaaca
    29281 gcggacaaaa aaaggagtac tagcgggtat cttaggattg gttacgaatc tgatcttgtt
    29341 tgtctctaag ctaatgatcg ggcttttttc aggaagtgtc tccattatgg ctgatgcagt
    29401 gaacagtcta tcagatactg cttcatccat attgactttg atcggattta aaattgctgc
    29461 aaagccagca gatcaggaac atccttatgg acacgaacgc ttcgaatata tcagtgggtt
    29521 atttgtttcg atcatcatta cttatgtagg atttcaattt ttagagtctt ctgctgaaaa
    29581 aatcatccat ccagaaaatg tctctttaac acccatcgtc ttttttgtcc taatcatttc
    29641 catctttttg aaatttgcac aaggcaaaat gtatcaggtt attgcgcaat ccattgattc
    29701 tgaaacattg agagctacag caacagatag ttataatgat gtctttacta cactggctgt
    29761 attgctttca gcagcaatcg aatgggtaac tggatggcgt attgatggat atataggttt
    29821 tgctttagca ttatatatcc tttatagtgg attgacgatg cttcgaaatt ttgtatacga
    29881 actattagga agtcgcccaa cgccagcaga gattgaagaa atggaagagc atttaaatgc
    29941 ttgcgatgat atacttggct atcatgattt attggtgcat aactatggac ccaataaacg
    30001 ctttgcatca gtccacatcg aagtagatga atcaatgaat ttgaatgtgg ctcataagat
    30061 tattgatctg atagaaaaag actttaaaaa gtctttggat gtggaacttg tctgtcactt
    30121 ggatccaatg cctgttgata acgaaaaata ctataatatc ttgcggcagt taaagcaaat
    30181 tcttcttgaa acaggtacag ggcttaatat tcatgatttt agagtaattc atcaaggaaa
    30241 aacgctgcaa tttgatgtag tggtccctga gaaattccag tttgttgata cagaactaga
    30301 aagaatctta cgtagtcaaa tagaagaaaa aatcggagat taccaattgg aaattacgtt
    30361 tgatcacaat tatcttttat attaaatgag aaactataaa aagactcctt aaagttttta
    30421 atttaaagag tctttttttc ttttaattgt ttatggagag cctgacgtgc aagattatct
    30481 gctcctttat ttttgttttc gggcaaccat tgcaggatca ataaagaaaa ttcggataga
    30541 agttcttgga ttctttgaag gtagggaagg aagagctcgt tcttcgttgc gtttttgtcg
    30601 accgtagaaa ctagtgtttt gctgtctgaa aaacagaaaa tcgtttcatt ctgtaagtcg
    30661 tttttcttta gaaattctaa agtttttaaa aagacttcga actctgcttg atggtttgtc
    30721 cctatagaaa gcgggataga tagctgttct tgtttacctt gatagacaat caagattcca
    30781 cctccactag gaccagggtt gccttttgta gaggcatcaa tgtatacttt taacataaaa
    30841 agagattctc ctttttgttc aaactaatgt tatggtaaga ttatatcatt actgaatcgg
    30901 tgagaggtta gagaatgaaa aaaaatattc gatggcagcc tgaactggca catacgatca
    30961 tctattggtc attgaccttg atcattctgt tttttagtct gattctttct ttagaaaaca
    31021 caggtcctta ttggaaaagc aattttgttt taggtatttt tcttgttttt ctatttctag
    31081 gtttgcggcg ttcttttatt ttaaaatcaa actctgtgat tatacgttat gctgcttttt
    31141 ggaaagaccg agaaatatct gtcggacaga ttcgatcttt gacgccaact aatactgggg
    31201 tgcaaatgat gttgcaagat gagaaagccc catctgtttt catgatgcga aaaaaagatc
    31261 aagtagtcct ttttgatcat tttgaaaaaa acggacccaa atcagaatca attccttatc
    31321 cttatacgaa aaaaagaaaa agctaatttt aaaaatcaga acctaaaaaa acgaattttt
    31381 acaggaaaag aaaagagtga gacgtacggt tcatcccgtt tagcgtctca ctctatttgt
    31441 ttatctattc atgcatacta gtatcggtag tctgaacgtt gcctttcttg acatgggcag
    31501 cttgtgttcc tcgattccct tcgatgatct caaattcgac cacttgattt tcttccaaag
    31561 ttttgaatcc ctcttcttca atcgcagtaa aatgtacaaa aatttcttct gtgtcatcat
    31621 aactgataaa gccgtatcct tttttgttgt caaaccattt tacaattcct gtggtcaaag
    31681 cactcacgac ctttctaaag tctgatcata gccttcatta taaagagtat ttataagtac
    31741 gtcaaataaa ttaaaaacct gtaattttat gttttttttc tgaataaaaa aaggtataat
    31801 tggcatatct taacaaagaa gatattttgt tgaataaagt tcgtgaaaag gggtaatcaa
    31861 atgaagaatg atctgcaaat ttcacaagaa acagagctag aaccgattgt tgaagtggct
    31921 agtaaattag atcttaagga agacgatttg gaactgtatg ggaaatataa ggcgaaaatc
    31981 aatttttctg gaatcaatcg atcaaaggat aacgctgagg ggcatttgat cttagttact
    32041 tccatcaatc caacaccagc tggcgaagga aagtcaacga tcactgtagg gttaggagac
    32101 gcgctcaata aaatcaataa aaaatctgtt attgcattgc gagaaccctc acttggtcct
    32161 gtgatgggaa tcaaaggtgg tgctgcaggc ggaggatatg cacaggtatt gcctatggaa
    32221 gatatcaact tgcattttac tggtgacatg catgctatca caacagctaa taatgcatta
    32281 tctgctcttt tagataatca tattcatcaa ggaaatgaat tgaacatcga tgcccgccga
    32341 gtgatttgga aacgggtagt cgatctcaat gatcgtgaac tgcgtaaagt aattgttggc
    32401 ttgggtgggc caatccaagg tgttccaaga gaagatgggt ttgatatcac agttgccagc
    32461 gaaatcatgg ctatcttgtg tcttgcggca gaccttgaag atttaaaggc tcgtcttgca
    32521 aggatcgttg tcggctatac atttgaccgt cagcctgtga ctgctggaga cttgaaagca
    32581 gaaggcgctc tggcattact gctgaaagat gcaatcaagc cgaatcttgt acaaacaatt
    32641 tacggaacac cagcttttgt ccacggggga ccgtttgcca atatcgctca tggctgcaat
    32701 agtattttag ctaccaagac agcattgaga ttagcagatt acgttgtcac agaggcagga
    32761 tttggtgcag atcttggcgg agagaaattt ttagatatca aagtacctaa cttgaaaaaa
    32821 gcgccggatg ctgtggtgat tgtcgccact gtccgtgcat tgaaaatgca tggcggaatg
    32881 aaaaaagatg aacttaaaaa tgagaatctt gatgctttga aagtaggttt tgcaaattta
    32941 aagcgccata tacgtaatat ggaacaatat cagctacctg tgatcgtcgc aatcaatgaa
    33001 ttcgtaacag atacagacaa tgaactcaca ctgttggagc acttgtgtga aaatcagggg
    33061 attcttgcca agagagcgag tgtatgggca gatggagcag aaggcggcgt tgatttagca
    33121 aaagctgtag tacgattgat tgatagaaaa gaagctaatt acaagccatt ataccgccta
    33181 gaagaaacga tacaagaaaa gacagaaaca atcgtcaaga aaatttacgg aggaaatggt
    33241 gttgttttca gcaaaaaagc gcaaaaacaa atagaagagt ttacgaagaa tggatgggac
    33301 aaactgccga tttgtatggc aaaaacacag tattcatttt ctgatgatcc ttcactgcta
    33361 ggtgcaccgg aagatttcac catcacgatc cgcgaaatca tcccaaaatt aggggcagga
    33421 tttttagtag ccttgactgg agatgttatg acgatgcctg gtttgcctaa aaaacctgct
    33481 gcattgaata tggacgtgac aaatgacgga gaagtgttgg gattgtttta agcttgatta
    33541 taatctacca tcgaaggagc tgtattttga ctacagcttc tttttttaga cgatagggaa
    33601 ttaattttgt ttacactagg atcattcttt ttctttattg tattttgagc tatactaaag
    33661 cataatagga gggtatgatg aaaacgacat ttataagaaa aaccggtttt tacggtatgg
    33721 gtagctcgat tcagctaaga atcaacaatg aaaaacgcag tgttctcagt catgaacaaa
    33781 agataacgct tgatctggaa gtgcccttta cgatgcaggt caccttttat tggctaaaaa
    33841 gccccgttta tacagtgaaa gaagcaaagc ctgcttatgt gatcacaatg aattacttga
    33901 ttcttcaaat ctatccgttt ttatttctag caactgggtt gggggccgtt ttcctacaaa
    33961 atttattttt cagttttttt gcagttattg tgttgattgc gttctttttc tatataaaaa
    34021 accgggcata tttgatcaag gaggaaaatt atggcaaact ctgatctttt tttaatcagt
    34081 ttcaatcgaa tcgaaaaatg gatgagagac gagatgggaa atgcacgaaa tatgggattt
    34141 accgaacttg tcagacgcct agctcaaaga aaacaattaa tgatcagaaa atacgaagat
    34201 gatcttttac agcttgctca gttaaggaat gcgatcgtcc atgatcggat cgcagtcgat
    34261 tttgtcattg cagagccaaa tgattggaca gtcaagcgaa tccagctgat cgaacaagaa
    34321 ctgattcagc ctgaaaccgt gctgcctcga tttgcaaaac gtgtaacagg ttttgaacaa
    34381 gatcttccgt tacttgaact gttaaagata gtagcagaaa aaaggtattc acaatttccg
    34441 ttatacaata aaggaaaatt tgtagcattg attactttac gtaatatcgg gttttggcta
    34501 gcaaaagaaa gccaaaaagg tccggttgat ttaacaaata aacgagcgtt ggatttgatc
    34561 atccaaaacg gaaagtacac aaattatcat tttgttccag ctaaaacaca tatttacgag
    34621 gttgaagcga tgtttcgtga gcaagggact ttggaagccg tacttatcac taaagatggg
    34681 aatcccgatg gtaatctgct cgggatcgta cgacctcgtg atatttataa acaaatagaa
    34741 aaggattgat tttttgttaa ttatttattg gatattcagc gctttgctag taggagtgga
    34801 tcagttggtc aaatggtgga tcactgataa tctagcgctt ggagagacga aaaatcttat
    34861 accaggtatt ttgtcactga atcatattcg aaacactgga gccgcatgga gtatgctaga
    34921 agggaagatg tggttcttta ctatagtgac actcattgca gtcgttgtga ttctcacatt
    34981 gatgattaaa aatcgtgaaa aaggtaatcg ctggttcatg attggattga gtttgattct
    35041 agctggtgcg attggcaatt ttatcgaccg tattcgtctt ggctatgtag tagatatgtt
    35101 ccagacagat tttatgagct ttccgatttt taacgtagca gatgtgacac ttgtatgtgg
    35161 agtgatttgt atgttaattt atattatttt agatgaaaag gatcaaagaa aaaaatgaca
    35221 gaacttaaaa cgacgattca ccatgaatct ggtaggatcg ataaagtatt gactaattta
    35281 ttcaccgatt acagtcgttc gcaaattcag ctgtggctga aaaatggagc ggtttctgtc
    35341 aatggacagc cagtcaaagc aaattacaaa gtaaaacaag gagacgagat tactgtcttg
    35401 atccccgaac cagaaacatt agatgtggtc ccagaaaata tcccgttgac gatcgtttac
    35461 gaagatgaag atgtagcggt gataaacaaa cctcaaggaa tggtcgttca tccttcagca
    35521 ggtcatacgc atggcacaat ggtcaatgcg ctgatgtacc atatcaagga tctttctacg
    35581 atcaacggcg ttattcgtcc aggtattgtt caccgaatcg acaaagatac ttccggactg
    35641 ctaatggttg ccaaaaatga ccaggcacac gaatcattag ctcagcaatt aaaagataag
    35701 acctctttgc gcaaatatgt tgctttagtc catggagaga ttcctcacga aaaaggaacc
    35761 atcaatgcgc caatcggccg ttcgaaggaa aaccggaaaa tgcaggcgat aagagaagat
    35821 gggaagtcgg ctgtcacaca ttttactgta ttggaacgtt tcgaaggatt tactctggtt
    35881 gaattaaaac tagaaaccgg aagaacacac cagatccgtg tgcatatgaa gtatatcggt
    35941 tatccgttag ctggagatcc agtatacgga ccgaagaaaa ctttgaaagg caatggtcag
    36001 tttctccatg cgcaaatgtt aggttttacg cacccgacta caaacgaaaa gatgatcttt
    36061 gaagcacctc ttccagaagt ttttgaaaaa actttagaaa aattaagaaa aaacattgca
    36121 ttttaattta gataacggta tagtaattaa caacgaaagc ataaaactta atagaattcc
    36181 tttaagttta gtcccgtgag gctaagaagg gtgggagcaa ggatacgtat ggtgcgccat
    36241 aagtagattg ctcattgaca tagtatagct atcagtgtat cagtatacgc tcagtctaaa
    36301 cattcctcct gtcctcttag ggcaggagtt tttttatgca aaaaagtagg gggaatgaaa
    36361 atgcaagcaa aagaagtagt agatcaggta accatgaagc gagcattgac tcgaattact
    36421 tatgagatta tcgagagaaa ccatagtatc caagatatcg tattggtagg tatcaaaaca
    36481 cgaggaatct acattgcatc aagaattgcc gaacgcctga agcagctaga agatatcgat
    36541 attcctgtag gagaactgga tatcacttta taccgagatg acaaaaaaga aaatccagaa
    36601 gaaccggaac ttcattcttc tgacatccca gtttccttag aaggaaaaga agttattttg
    36661 attgatgatg ttttgtatac aggacgaacg attcgagcag caatggacgc agtaatggat
    36721 tttggcagac caagaaaaat ttcattagca gtcctggtcg atcgtgggca tcgagaattg
    36781 ccaatccgtg cagattatgt tgggaagaac attccaacat ctcgggcgga agagatttta
    36841 gtagagatgc aagagcttga tggacaagat cgcatcatga ttttgaaaga ggaagattag
    36901 aggagagaga aacatgtcgg agaaaaaagg atttcataat gatgacgtgg tgttagatat
    36961 ccacgatcgc ccaaccccag gacattgggt aggattaagt ttacaacact tgttcacgat
    37021 gttcggagca accgtgttag tccccatctt agtaggtatc gatccaggta ttgcgctagt
    37081 cagttcagga ctaggtacat tggtgtactt atttactact aaaggaaaga tacctgcgta
    37141 cttaggaagc agcttcgctt tcattgcagc gatgcagctt ttgatgaaaa cagatggtta
    37201 tccagcgatt gcacaaggag cagtcacaac tggattagtc tatttgattg ttgcgctgat
    37261 catcaaaaag atcggttcag catggctgga taaaatcttg cctccaatcg tcgtcggtcc
    37321 agtcatcatg gttatcgggt tgggacttgc ggcaaatgca gcaacaaatg ctatgtataa
    37381 tcagggcacg tatgatttca agtatattgc tgtagcgctg atcacattag gattaacaat
    37441 tttcttcaac atgtggctaa gaggttttct cggattgatt ccgattttac taggtattat
    37501 tagcggatac atcgtagcct tgctgtttgg aatcgtggat actaagccaa tcgtagacgc
    37561 tgcctggttt gctgttccta actttgagat tccctttgta caatatgaac caaagctata
    37621 tcttggagca atcacaacaa tggcacccat cgcttttgtg acgatgacag agcatatcgg
    37681 ccatcttatg gtattgaata aactaactaa acgtaatttc tttgaagaac cgggtcttca
    37741 taagacgttg acaggtgatg gattagcaca aatcgtggca gcctttgtcg gaggtcctcc
    37801 tgtaacaagt tacggtgaaa acattggtgt tctggctatc acacgtgtcc atagcgtgtt
    37861 tgtgatcggt ggagctgcag tatttgcagt cattttagga ttcgtaggaa aattaagcgc
    37921 tttgatttta agtatccctg gaccagtcat ctcaggaatc agttttatct tatttggagt
    37981 aattgccgca agtggaatga agattttaat cgagaacaaa atcgattttg ataaaaagaa
    38041 aaatctgttg attgcttcag ttatcttagt agtaggaatt ggcggactcg tctttgaagt
    38101 aggcacattt acattgtcgg caatggcatt agcaactgta cttggcatca ttttaaatct
    38161 tatcttacca gaaacttcac gaagcgaaga aaactaagga ggaaacatta tgatcatcaa
    38221 atcagaacgt atcagcttaa agcatttatt gaccgttgag gcacttactg atcaagaagt
    38281 tatgggcttg atccgcagag ggcaggaatt caagcaaggt gctaattgga ctccgcaaaa
    38341 gcgccagtac tttgctacaa acctcttttt cgaaaatagt actcgcacac ataaaagctt
    38401 cgatgtagcg gagaaaaaac tagggctaga agtgattgaa tttgaagcaa gcacaagctc
    38461 tgtccaaaaa ggggaaacac tatacgatac agtactgacg atgtcagcat taggcgtaga
    38521 cgtagcggtt atccgtcatg gtgatgagaa ctattatgac gaactgatac aaagtaaaac
    38581 aatccagtgt tcgattatca atggaggaga cggaagcgga cagcatccaa cccaatgctt
    38641 gcttgatttg atgaccattt atgaagaatt tggtcatttt gaaggtctga aagttgccat
    38701 cgtaggtgat atcactcact ctcgcgtagc taaatccaat atgcagatgt taaaacgtct
    38761 aggcgcacaa gtattcttct caggtcctag agaatggtac gacgatgaat tcgaagtgta
    38821 cggacattat atgccattgg atgaactact agatcaagtt gatgtcatga tgatgcttcg
    38881 ggtgcaacac gaacgccatg atggaaaaga aagtttctca aaagaaggct accatcagga
    38941 acatgggtta acagttgagc gtgcgaagaa gttgcaaaaa catgcaatca ttatgcaccc
    39001 agcaccagtt aaccgtgatg tagaattagc tgattcatta gtggaaggat tacagtcgcg
    39061 gatcgttcag caaatgagca atggagtatt cgtcagaatg gcaatcttag aagcagtgct
    39121 aaatggaaaa gcgtaattat aggaggatga aaaaatgaaa acactcatta aaaacggtca
    39181 gatcaataca cgaaaaaata tgacgacacc agcagaaatt tggatagaag atgggcgaat
    39241 caaagcaatc ggtacaggat tctcagaagc cgaatttgat gaagtgtttg atgcaaaggg
    39301 acaattgatc acacctggtt tagtggacgt ccatgttcat ttgagagagc caggatttac
    39361 atacaaggag acgatcgaag ctgggactcg ctcagctgct cgtggcggtt tcactactgt
    39421 atgcgcgatg ccgaatttaa atcctgtacc tgatacggca gaaaagctga gacaagtata
    39481 tgacatcatt agaaaagatg cagttgtgaa ggtattgcag tatgcgccaa tcacagaaaa
    39541 tctgcgaagt gagaaattag ttgatcaaga agcactgatt gaagaaggtg cttttgcttt
    39601 cacaaatgac ggtgtaggtg tccaaacagc tgggacgatg tatctggcta tgaaagaagc
    39661 tgcaaaaaac aataaagccc tagtagccca tacagaagat gaatctttat tatttggcgg
    39721 cgttatgcac gctggcaaga aggcagaaga acttggtctt cccgggatct taagcgtaac
    39781 cgagtcttct caaattgcta gagatctgtt gctagctgaa gcaactggtg ttcattacca
    39841 tgtttgccat gtttctacta aagaaagtgt ccgtgtaatc agagacgcaa aaaaagcagg
    39901 aatccacgta acagcagaag tttcacccca tcatcttatt ttgatcgacg aagatattcc
    39961 cgaagatttc ggcttttgga aaatgaaccc gccgctaaga ggacgggagg acagagaagc
    40021 tttgatcgaa ggcttgcttg acggtacgat cgattgcatc gctacagacc atgcccccca
    40081 tggattagaa gaaaaaagcc aaagtttcat gaaatcacca tttggtatcg taggaagcga
    40141 aacagcgttt caactgatct atactcattt tgtagaaact ggtcgattca cgttggaaca
    40201 agtgatcaat tggttggcag ttaaaccggc tgagattttt ggtttgaatg caggaacgct
    40261 aacggttggt gcccccgctg atgtagcagt ttttgatatt acacaaactt gcacgatcga
    40321 taaagaagac tttttatcaa aaggggagaa tacacctttt atcggctgga aagtaaaagg
    40381 cgaaacacaa atgacttttg tcaacggaaa acttgtgtgg cagaagggag aataaaagta
    40441 tggaacgttt gcttatatta gaagatggaa cagttttcga aggaaaggct tttggtgcag
    40501 atgctagtgt cttcggagaa atcgttttta caacaagtat gactggttat caagaaacta
    40561 tcactgatca aagttttaac ggtcaaatca ttacatttac ctatcctatg gtgggtaatt
    40621 atggggtaaa tagagatgat tatgaatcga tttctccaac atgcaaagga gtagtagtaa
    40681 aagaacatgc acgtgtagct tcaaactggc gaaaccaaat gacgctagat tcatttttga
    40741 aacaaaaagg aattcctgga atatcaggta tcgatacgcg cgcccttact cgcaaaatcc
    40801 gtgaagtcgg aacgatgaaa gctagtatag ttgatgctgg agattccttc gaacatgcat
    40861 ttgatcagct gaaagcaacc gttctagcaa ccaatcaagt gcagcaagtc tcaactaaca
    40921 aaccttatcc aagtccagga acaggtcgga aagtcattgt cattgacttc ggcttgaaac
    40981 atagtatttt acgagaattg agtaaaagaa actgtaacct cacagtactt ccctataaca
    41041 cagatgccga gacgatttta gcgctatcac cagacggagt catgctgacg aatggacctg
    41101 gtgatccaaa agatgtgcca gaagcaatcg aaatgatcaa aaaaatccaa ggaaaagtac
    41161 cgatttttgg tatttgtctc ggtcaccagt tatttgcttt agccaacggt gcagatacct
    41221 ataaaatgaa attcggacat cgaggattaa atcacccggt acgagaagta gcgaccggaa
    41281 gaattgattt tacttctcaa aatcatggct atgcagtcga tgaagccacg attgatccag
    41341 aaaaactcat cgtaactcat gtagaagtaa atgacggaac agtagaagga gtcaaacaca
    41401 gagattatcc cgctttcagt gtgcagtttc atccagatgc tgcccctggt ccgcatgatg
    41461 cactacattt atttgatgaa tttatggaaa tgatggacgc aggaaaggag cagtaaacag
    41521 atgccgaaac gtacagatat caagaaaatt atggtgattg gatctggtcc gatcatcatt
    41581 ggtcaagccg cagaatttga ctacgcagga acacaagcct gccttgcctt aaaagaagaa
    41641 ggatatgaag tggttttagt aaattctaat cctgcgacga tcatgacgga taaagaagtg
    41701 gcagatcaag tatatatcga accgatcaca cttgaatttg tatcaaggat tcttcgaaaa
    41761 gagcgtccag atgctctcct tccaacttta ggcggacaga caggcctcaa tatggcaatg
    41821 gagctggcag catcaggtat tttagaagaa ttgaacatag aacttctagg aacaaagctt
    41881 tctgcgatcg atcaagcaga agatcgtgat ctattcaaac agttgatgaa agaattgaat
    41941 cagccaatcc ccgagtctga aatcgtcaat acagtagaag aagctgtaga ttttgcaaat
    42001 aaaatcggct atcctgtcat cgttcgtcca gcctttactt taggcggaac tggtggaggt
    42061 atgtgtgaaa atgaagaaga attacgtatc gtatcagaaa atggattaag tctttccccc
    42121 gctacccaat gtttgattga acgaagtatt gcaggtttta aagaaattga atatgaagtg
    42181 atgagagatt cggctgataa tgcaatcgtt gtctgcaata tggaaaattt tgacccggtg
    42241 ggtatccata caggtgattc tatcgtattt gcccctagtc aaacattgtc tgaccatgaa
    42301 tatcaaatgc ttcgtgatgc ttctctttcg attattcgag cacttaagat cgagggaggc
    42361 tgtaatgtcc aactcgctct tgatccacat agttttaatt attatgtgat tgaagtcaat
    42421 ccgcgtgttt ctcgttcatc tgcgttagca agtaaggcga ctggctatcc gatcgctaag
    42481 ctagctgcta aaatagcgct tggattaaca ttagacgaaa tgaaaaatcc tgttacagga
    42541 acgacttttg cagaatttga acctgcatta gattatgttg tagctaaaat tcctcgctgg
    42601 ccgtttgata agtttgaaaa cggagaacgg cttttaggca cgcagatgaa agcaactggt
    42661 gaagtgatgg ctatcggtcg gaatatcgaa gagtctttat tgaaagctgt gcgttcttta
    42721 gaaatcggta cacaccatat cgaacttcct gagttggcag acatcacgga cgatgaccta
    42781 atccaaaaag tggttcgagc acaagatgat cgcctgtttt atgtagctga agctattaga
    42841 agagggtaca ccatagaaga attagcggat ttgacaaaaa tcgatttatt ctttttagat
    42901 aaactgttgc atatcgtaga aatcgaacag cagttgaaac agcagccgtt ggatgacaag
    42961 atattgaaaa cagcgaaaca aaacggtttt acagacaaaa aagtggcgga gctgtgggaa
    43021 ataaacgaag aagccgttcg ccagatgaga aaagaaaatg atttgattcc tgtatataag
    43081 atggttgaca cttgtgcagc ggaattcgaa tcaaaaacac cttattttta tagttcctat
    43141 gaatatgaaa atgaaagttc acgatcagaa aagccctcaa ttctagtact gggatctggt
    43201 cctatccgta tcggacaagg ggttgaattt gactatgcga ctgtgcattc tgtcaaagca
    43261 atccagcagg caggatacga agcaatcatc atgaacagca atcctgaaac agtttcgact
    43321 gacttttcta tttcggataa attatatttt gagccattga ccttcgaaga tgtgatgaat
    43381 gtgatcgagc tagaacagcc gataggagta atcgttcaat ttggtggtca gacagcaatc
    43441 aacttagccg aacctttgac tgctgctggt gtaaaaatct tagggacaac gatcgaagac
    43501 ttggatcgtg cggaaaatcg tgatttattc gagcaagcgc tgaaagagtt ggatattcct
    43561 caacctttag gggatactgc tacaagtaaa gaagaagccg tggttatcgc ttcgagaatt
    43621 ggttatccag ttcttgttcg tccaagctat gtattaggcg gacgtgcgat ggaaatcgtt
    43681 gaaaaccagc gagatttaga agattacatg gaacatgctg tgaaagcatc tccagaacat
    43741 ccagtactag tggaccgtta cttgcttggt ggtgaatgcg aagtggatgc gatttgtgac
    43801 ggtgaaacag tactcatccc agggatcatg gagcatattg agcgtgctgg tgtccattca
    43861 ggggattcaa tggctgtgta tccacctcaa acattatctg aatcgatcaa gaaaacgatc
    43921 gaagattaca cgatccgctt agcattaggt ttgaattgtg tcgggatgat gaatatccag
    43981 tttgtcatcc atgaaaatga ggtttatgtg atcgaagtca atcctcgagc aagtcggact
    44041 gttccattct taagtaaggt gacaggaatt ccaatggcac aaattgcgac gaaagctatt
    44101 ttgggagaga aactaaagac acttggttat gaaaacggac tttatccaga aacaaaagaa
    44161 gttcatgtta aagcacctgt cttttctttt acgaaattgc agaaagttga tacttactta
    44221 gggcctgaaa tgaaatcgac tggagaagta atgggatctg acagacaatt agacaaagct
    44281 ttgtacaagg cattcgaagc ttctggatta cacttgcctg aatatggagc cgtattattt
    44341 acgattgcgg acgaaacaaa agaagaagca ctagaattag ctaaacgttt tacagaaatt
    44401 ggctatagtc tgataggaac gaaacataca gctgcttatt tcgaaaaaaa tggtctggtc
    44461 gttgaaacag tagcgaaaat ttctgaggaa gcagcagaaa aaaatgtagt cgacttaata
    44521 agagatggta aaacacaagt ggttgtcaat acgattgata aagatcgggg aaatgcttct
    44581 aaagacggtt tcatcatccg aagagaagca gttgaacatg gtattccatt gtttacttct
    44641 ttagacacag cagatgcgat cgttcgagtg atggaatccc gtgctttttc aactaaccct
    44701 atttaacggc gagcacatgt taagacaaaa agacgaaaaa ccgttgcagg aggggacaga
    44761 tgaaacaaga aatgatggaa atcgttcagc aaaaacagct ggcaccaaag atttttgaaa
    44821 tgacattgaa aggtgatcta gtaaaagaaa tgcagcaacc aggacagttc ttgcatattc
    44881 tcgtgccgcg tgcagacttg ctattacgcc gaccgattag cttgaatcat atcgaccacg
    44941 aaacaaatac ttgccgtatc atttatcggg tggaaggtga ggggactaga gtcttctcta
    45001 ctctttctgc cggagaccat ttagatgtca tgggtccttt aggcaatggt tttgatttgt
    45061 ccaatcttca tacaggagac gaagtattta taatcggtgg aggaatcggt gtccctcctc
    45121 tatatgagtt atcaaaacag ttagtcgaaa aaggaattaa gccaacgcat tttttaggtt
    45181 ttgccacaca tgaagtcgtt tattatgaaa aagaatttaa tcgtctagga gaaacaagaa
    45241 tcgctactga cgatggaaca tacggtatgc acggaaatgt cggtaattta ttgttaggaa
    45301 cgaagacgga acctacagct gtttttgcat gtggttcaaa cgggttattg aagacagtgg
    45361 aacagctgtt ttcttcgcat caaaatgtcc agttatcatt agaatcccgc atggcatgtg
    45421 ggataggggc ttgctatgct tgcgtttgtc atacgccgga agatgaaaca ggaacaaaaa
    45481 gtgtgaaagt ttgtgacgaa ggaccgattt ttaaaatagg agaggtaatt atatgaatga
    45541 gaatccttta gcggtcaaac ttcctggcct agaattaaaa aatcctatca tgccggcaag
    45601 cggctgtttc ggttttggca aagaatacgg aaaatactat gatttgaatc agcttggatc
    45661 gatcatggtc aaggcaacga ctccaaatgc ccgctttggc aatcccacgc caagagtggc
    45721 ggaaacacca tctggcatgt tgaatgcaat tggtttgcaa aatcctggtc tagacgttgt
    45781 tatgcataaa cttcttccag aattagagga gtacaatgag ttacccatca tcgcaaatgt
    45841 tgctggtgct tgtgaagaag actatgtaga agtttgtagc aaaattggcg aagcaccaaa
    45901 tgtgaaagca atcgagctga acatttcttg cccaaatgtc aaacatggcg gaattgcttt
    45961 tggaacagat tctgatgttg cttttcaatt aacacaagca gtaaagaaag tttcttctgt
    46021 tcctgtctat gtcaaattgt ccccaaatgt aacagacatc gttccaatcg ctcaggcaat
    46081 cgaagctgga ggggcagatg ggtttacgat gatcaatact cttcttggta tgcggattga
    46141 ccttaagaca agaaaaccga ttttagccaa tcaaacaggt ggactctccg gaccagcaat
    46201 caagcctgtc gctattcgtt tgattcacca agtagcaagt atatccagcc ttccaatcat
    46261 tgggatgggc ggcgtgcaga cagtggatga tgttcttgag atgtttatgg ctggagcaag
    46321 tgctgtagct atagggaccg cgaatttcac cgatccgtat atctgtccta aactaattaa
    46381 agagctccct gtgcgaatga gcgaactagg catcgaatcg ctggaaagat taatcaaaga
    46441 agtgagagag gaacgtgaac gatgaatcaa ctagaccaac gaccaattat tgcattagat
    46501 ttttcaacta ggcaagaggt agaagacttt cttcgatttt tcccgaaaga agaaaaattg
    46561 tttgtgaaaa tcggaatgga attgttctac caagaaggac ctgaaatcgt acgttatcta
    46621 aaagaggctg gtcataatgt atttttagat ctgaaacttc acgatattcc gaatacggtc
    46681 gaaaaagcaa tgcggggatt ggcaaagctt ggtgttgatc tcacctgtgt tcatgccgct
    46741 ggcggtattc gtatgatgga ggcggcaatg cgaggattag aagaggggac tccagaaggt
    46801 ggaaaacgtc cgctacttct agctatcact cagcttactt ctacaagtga agaagaaatg
    46861 catgccgatc aactaatcga agtttcttta gaaaaaagtg tgatccatta tgctagctgt
    46921 gccaaaaaag caggattaga tggtgtggtt tcttctgctt gggaagtaga agcgatcaaa
    46981 gaaatggcag gagacgaatt tgtttgctta actccgggta taagaccaga aggaacagtc
    47041 gcaggagatc agacaagagt agtcacacct tctcaggcaa aaaagatcgg ctcaactttt
    47101 atcgttatag gtcgaccaat cactcagtca actaatcctt ataaagcata tcaaacgatt
    47161 caaacagaat ggagctaacc taaaatgaat gttgaacaaa gtatcgcaaa agacttatta
    47221 gaaattgaag cagtattttt aaatccaagt aatcctttta catgggcaag tggaatcaaa
    47281 agcccaattt actgtgataa ccgaatcacg atgagctatc caaaagtccg aaaagagatc
    47341 gcaaaaggat tagcaagcaa aatcaaagaa gcttaccctg aagtacaagt gattgctgga
    47401 actgcaacag caggaatccc tcatgccgca tgggtagcgg aaatactgga tttgccaatg
    47461 gtctatattc gaagtaaggc gaaagatcat gggaaaggta accaaatcga aggacgtata
    47521 tttgaaggtc agaagatggt tgtgatcgag gatttgattt ctacaggcgg aagtgtcttg
    47581 gaagcagctg aagcagcaaa aagagaaggt gcggatatcc ttggtgttgc ggctatcttt
    47641 acgtatgagt tgccaaaagg aaaagcaaat ttcgaaaaag ccgaaatccc tcttatgacg
    47701 ttgaccaatt attctgtatt gattgaagca gctttagaag atcgttatat cgatgagcaa
    47761 gaattaactc tgctaaaaga atggaaaaaa gatcctgaaa attggcagat ttaagcgcgc
    47821 ttgatgaaga tagagaaatc cgatttgtgt ctccagaatc atcaattgga atagcaaaaa
    47881 tccaaaaact gattgcgaga agcatctttt catcaggtat gaaaagaaac ttctatcgtg
    47941 atcagttttt gattttttta aaattcattt ttctaagact ggttagcaga aagaagagaa
    48001 taatctcttc tcttttttct attatatata gattttctct attttttggt aagggagctg
    48061 tttcaaaaat ggtaaagagc ggaaatgttg cgaatatcag ttatttaatg atatgatatt
    48121 cggaaaaaga aatctcaaag ggaggtaact tttatggggt tgaatgaatt tattttagga
    48181 ttgaacaatt tagagacgat cacaagggca cctggatttt ttaaatatac agagcataca
    48241 gttgcagcac attcgtatcg tgtagcatct attgcgcaag ttctaggaga cattgaagaa
    48301 gcgaatggac aaccgatcga ttggaaactg ctctatgaaa aatcattgaa tcatgactac
    48361 acagagcgtt ttattggtga tatcaaaacg ccggtgaaat acgcaagcaa agagttaaga
    48421 gggatgctgc aaacagttga agaaaagatg acggacgaat tcatcaacca agagattcca
    48481 gctgagttcc aagaagttta tcgccgtcgg ttgttcgaag gaaaagatga aaccgtagaa
    48541 ggagaactat tagccatttc agataaggtt gaccttcttt atgaatcttt tgatgaaatc
    48601 atcaaaaata acccggaaaa agtttataag gagatgttca ttgaggcagt gactacgatt
    48661 aaaagctttg aacatcggcc ttctgttcag tatttcttta aagagatatt tccggaacta
    48721 ttgaaccagg aattttatgg aaaagaggcg tttttagaaa ccgtctcaac gattgtaaag
    48781 aaagaagaaa aataaaaatg atatgagatg cagggggaag tatagttgaa aaagaatatg
    48841 gatgtcgaat ggggctattc tggagacaaa ggtccggaaa attgggcgag tttatgtgat
    48901 tggtttgcga gaggtgcgga atttccattg cagtcgccta ttcatttgac caagggggaa
    48961 gaaaccaaaa tagaaggaga cacgctttcc tttcattatc aaaagcaacg gtttactgac
    49021 aaggaattca aaaacacgat tcatttagta ccttttgacc aactcagtta tgtagagttc
    49081 aagaaagaac gctattattt gacagatatt catttccact tgccttctga acatatcatc
    49141 aacggaaaac aggaagatat tgaatttcat tttgttcata tgaacagcaa aaaagagaat
    49201 ctagttgtgg gtgtcatgta ccagctaatg gtacaggaag gttggctgta taatcaagaa
    49261 aatggggaaa gttggaactt agaaaaacat gagcattggg taaatccgga tgtgttcttt
    49321 ccggagcaaa aaagccattt ccattatatt ggctcattga caactccgcc taccagcggg
    49381 ccaatccaat ggtttgtaat ggatcatgta ggaaaactga accaagagtt tcttctcccc
    49441 ttcgatggtc agtatgctcg accgaacaac cgaccaatcc agcccttgaa tggaagagaa
    49501 atttattatt tcgaggagtt tgagcaatga atattcagca gatgaaatac gtggtcgctg
    49561 tagcaaacaa tggtagtttc agagaagcgg caaagaaaat gtttgtctcc cagccaagtc
    49621 tttcagctgg tatcaaagaa ttagaaaccg agttagggat cacgctgttt actcgaacga
    49681 atcgaggggc atatttaaca gaagaagggc aagacttctt aaatcgtgcc gagaaaatat
    49741 tggtccagct tgatacatta gaaactcact atctaacgag tgaaaagaga gacaattttt
    49801 cgatcgctgc ccagcattat gatttcttag gtcctttgac cgcacagcta ctcgaggaat
    49861 tcaaagacga gatcaaacaa tttcggatca tggagacgac aacagcgaga gtgatcgaag
    49921 aagtcaaaga gcagcacagt gagatcggca ttatttatat gaatgagcat aatcaagctg
    49981 gtatcagaag atatttacaa caaggagagc taagtggccg cagcattgga aaattcaaaa
    50041 cgcatatttt tttaaggcaa ggacatccat tagctgaaca cgcagagatc accaaagaag
    50101 aattgttatc ttatcctcaa gttcgtttta cacaggatgg aaataatttc ccgtattttt
    50161 atgaagacct tatcgagatc cctgatcaag aaacagtgat ttatacgagt gatcgaggta
    50221 ctttgatgaa tatcgttctt gaaactgatg cttacgcttc aggatctggt attgtgattg
    50281 gagggattcg cgagcacttg cggttaatcc ctttggcaga aggcgaacta aatgaatttt
    50341 atatcattta ttcggcgaaa cgtccgctca gtgaaatcgc tcaaagattt atcaaagagc
    50401 ttactcggtt actggaccag cagagctaag aaaagcagat gagaacgctg tttattgaac
    50461 accgttgatc aaaaaagagg ggttgtgaca gaattttgtc acaactcctc tttttgtagc
    50521 aaacaattta taactaattg ctgttgtggg aaagtacgca aatatgattc tagaaacact
    50581 cattgattac ctgttttgtt tcatagctgt aattttttct tcttctggtg tcacgataat
    50641 cgtcttttgg ttttcataaa taacataacc aggcttggcg ccatttggtt ttcggatatg
    50701 ctttacctgt acaagatcaa ccggcacttg ggcagagtat cggtattttg aaaaataggc
    50761 agctaactcg gcagcttctg taattgtttc atctgagggc ttgtcacttt taatgatgac
    50821 gtgcgaaccg ggtatatttt ttgcatgaag ccaatagtcg gtttttttag ctgtcttcat
    50881 tgtcagctgg tcattttgaa gattgttttt accaacaaga atgagcgttc cgtcagaaga
    50941 aaagtattga tcaggctgag atggtttctt acgtttttgt ttttttaagt tcttcttttt
    51001 caggtatcct tctgaagtca attcttcttt aattgcttcg atatccattg ggccggcaat
    51061 ttccagttga gataagacag attcaagata ttggatttca tctttggctt cttggatttg
    51121 ttcgccaatc agcttaacag cgttcttcag cttttggtat cgatggaaat atttctgtgc
    51181 attttgattt ggtgtcaatg ccggatctag tgctattttg attggccgat cttcttcata
    51241 ataattaggt aaaacaactt cttttgcgcc gcgtggtact tgtgtcagaa aagtagtaag
    51301 tagttcgcca tttctacggt aattctcagc gttttcggat tctttgagtg tttgttcacg
    51361 ttttttcaat ttatttttat ttcgttttag ttcattttcg atttttcgaa tcagttctcc
    51421 gccttgttgt tttgcccgat ctttctctgc tttttcgtga taatagctat ctaataattg
    51481 actgagtgtt ggataagctg ttacagcaga tgcctgttcg gacaatacct gataagcaat
    51541 cggtgtgaaa aaatctttat tttccgtttc gtaaaaagta ggaataggct gatggctgat
    51601 ggctgagaaa aattcgtccc atacaaccat cttttcatta ggacgttcca gtaatctttt
    51661 ggtcagctct tgagcgatat cgaaaccgat tccctgaaac tgacgttgga ttgcttttgg
    51721 atctagttca gtttgggaaa gccgctgaaa gattttttct ttctcggagc taaatgggtt
    51781 caagacctct tgttttggcg gagcaacata ttctacacca ggaagcaaag aacgataagt
    51841 gttttgagag cttccaatgt gtttgatcgc atccagtatt tttccagttt ctcgattaac
    51901 taagacgatc gtactatgac gtcccattaa ttcaacgatc aatatgatat tttgtagatc
    51961 acccaactcg tttcgtttag caaagtggaa atggatcact cgatcatttt cgatttgttc
    52021 gatgtcttct aaaatagcgc catccaaata ttttcgaagc atcatcacga agttaggcgg
    52081 attatcagga ttctgataat cgatttgggt aatctgtaca cgagcataac ttgggtgcgc
    52141 agataataat aatcgatggt ttttgccgcg tgaacgaatc accagcacga tttcattttc
    52201 ataaggctga tggattttcg agatccggcc agatagtaac gtttcacgca attcgttgac
    52261 catcgcgtgg gtaaatacac catcaaagga catggttcat tcctcatttc ttttcataag
    52321 tatattcatt ataacgaaaa aaagaagaaa aaccaatttg gccgaatagt aaagcaaaag
    52381 tctagaaaaa tcttggacta attacataaa aaacagtgtt tctatcgttg aaatccaaat
    52441 ttttaggt
//
LOCUS       DAZABD010000012        50091 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000012 DAZABD010000000
VERSION     DAZABD010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50091
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.012"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..554)
                     /locus_tag="ACHALG_000779"
     CDS             complement(<1..554)
                     /locus_tag="ACHALG_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFN2586210.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVFLLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIE"
     gene            complement(708..1238)
                     /locus_tag="ACHALG_000780"
     CDS             complement(708..1238)
                     /locus_tag="ACHALG_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFN2586211.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(1337..1687)
                     /locus_tag="ACHALG_000781"
     CDS             complement(1337..1687)
                     /locus_tag="ACHALG_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HFN2586212.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(1844..3373)
                     /gene="ahpF"
                     /locus_tag="ACHALG_000782"
     CDS             complement(1844..3373)
                     /gene="ahpF"
                     /locus_tag="ACHALG_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFN2586213.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(3383..3946)
                     /gene="ahpC"
                     /locus_tag="ACHALG_000783"
     CDS             complement(3383..3946)
                     /gene="ahpC"
                     /locus_tag="ACHALG_000783"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFN2586214.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(4090..4752)
                     /locus_tag="ACHALG_000784"
     CDS             complement(4090..4752)
                     /locus_tag="ACHALG_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFN2586215.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            4936..6582
                     /locus_tag="ACHALG_000785"
     CDS             4936..6582
                     /locus_tag="ACHALG_000785"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFN2586216.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            6573..7655
                     /gene="uxuA"
                     /locus_tag="ACHALG_000786"
     CDS             6573..7655
                     /gene="uxuA"
                     /locus_tag="ACHALG_000786"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFN2586217.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(7763..9316)
                     /locus_tag="ACHALG_000787"
                     /pseudo
     CDS             complement(7763..9316)
                     /locus_tag="ACHALG_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            9464..10605
                     /locus_tag="ACHALG_000788"
                     /pseudo
     CDS             9464..10605
                     /locus_tag="ACHALG_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(10731..11810)
                     /locus_tag="ACHALG_000789"
     CDS             complement(10731..11810)
                     /locus_tag="ACHALG_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFN2586218.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(11913..12353)
                     /locus_tag="ACHALG_000790"
     CDS             complement(11913..12353)
                     /locus_tag="ACHALG_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFN2586219.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(12410..12820)
                     /locus_tag="ACHALG_000791"
     CDS             complement(12410..12820)
                     /locus_tag="ACHALG_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFN2586220.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(13000..13434)
                     /locus_tag="ACHALG_000792"
     CDS             complement(13000..13434)
                     /locus_tag="ACHALG_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFN2586221.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(13534..14382)
                     /locus_tag="ACHALG_000793"
     CDS             complement(13534..14382)
                     /locus_tag="ACHALG_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFN2586222.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            14771..16537
                     /locus_tag="ACHALG_000794"
     CDS             14771..16537
                     /locus_tag="ACHALG_000794"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFN2586223.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            16639..17898
                     /locus_tag="ACHALG_000795"
     CDS             16639..17898
                     /locus_tag="ACHALG_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFN2586224.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            18045..19346
                     /locus_tag="ACHALG_000796"
     CDS             18045..19346
                     /locus_tag="ACHALG_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN2586225.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            19349..20200
                     /locus_tag="ACHALG_000797"
     CDS             19349..20200
                     /locus_tag="ACHALG_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFN2586226.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            20213..21028
                     /locus_tag="ACHALG_000798"
     CDS             20213..21028
                     /locus_tag="ACHALG_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HFN2586227.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            21179..22345
                     /locus_tag="ACHALG_000799"
     CDS             21179..22345
                     /locus_tag="ACHALG_000799"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFN2586228.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            22575..23222
                     /locus_tag="ACHALG_000800"
     CDS             22575..23222
                     /locus_tag="ACHALG_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFN2586229.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            23306..24250
                     /locus_tag="ACHALG_000801"
     CDS             23306..24250
                     /locus_tag="ACHALG_000801"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HFN2586230.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            24385..25836
                     /locus_tag="ACHALG_000802"
     CDS             24385..25836
                     /locus_tag="ACHALG_000802"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HFN2586231.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            25857..26693
                     /locus_tag="ACHALG_000803"
     CDS             25857..26693
                     /locus_tag="ACHALG_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFN2586232.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            26693..27352
                     /locus_tag="ACHALG_000804"
     CDS             26693..27352
                     /locus_tag="ACHALG_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFN2586233.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            27478..28167
                     /locus_tag="ACHALG_000805"
     CDS             27478..28167
                     /locus_tag="ACHALG_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2586234.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            28173..29129
                     /locus_tag="ACHALG_000806"
     CDS             28173..29129
                     /locus_tag="ACHALG_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HFN2586235.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHRIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            29173..29685
                     /locus_tag="ACHALG_000807"
     CDS             29173..29685
                     /locus_tag="ACHALG_000807"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN2586236.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(29941..30489)
                     /locus_tag="ACHALG_000808"
     CDS             complement(29941..30489)
                     /locus_tag="ACHALG_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFN2586237.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(30578..31759)
                     /locus_tag="ACHALG_000809"
     CDS             complement(30578..31759)
                     /locus_tag="ACHALG_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HFN2586238.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(31995..33503)
                     /locus_tag="ACHALG_000810"
     CDS             complement(31995..33503)
                     /locus_tag="ACHALG_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HFN2586239.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            33662..33853
                     /locus_tag="ACHALG_000811"
     CDS             33662..33853
                     /locus_tag="ACHALG_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2586240.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            34056..34973
                     /locus_tag="ACHALG_000812"
     CDS             34056..34973
                     /locus_tag="ACHALG_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFN2586241.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            34966..36039
                     /locus_tag="ACHALG_000813"
     CDS             34966..36039
                     /locus_tag="ACHALG_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2586242.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            36187..36705
                     /locus_tag="ACHALG_000814"
     CDS             36187..36705
                     /locus_tag="ACHALG_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2586243.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            36686..37078
                     /locus_tag="ACHALG_000815"
     CDS             36686..37078
                     /locus_tag="ACHALG_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HFN2586244.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            37154..38089
                     /locus_tag="ACHALG_000816"
     CDS             37154..38089
                     /locus_tag="ACHALG_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFN2586245.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            38222..39001
                     /locus_tag="ACHALG_000817"
     CDS             38222..39001
                     /locus_tag="ACHALG_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFN2586246.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            38994..39311
                     /locus_tag="ACHALG_000818"
     CDS             38994..39311
                     /locus_tag="ACHALG_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFN2586247.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            39555..41165
                     /locus_tag="ACHALG_000819"
     CDS             39555..41165
                     /locus_tag="ACHALG_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFN2586248.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            41322..42170
                     /locus_tag="ACHALG_000820"
     CDS             41322..42170
                     /locus_tag="ACHALG_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFN2586249.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            42401..42880
                     /locus_tag="ACHALG_000821"
     CDS             42401..42880
                     /locus_tag="ACHALG_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="HFN2586250.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            42913..45141
                     /locus_tag="ACHALG_000822"
     CDS             42913..45141
                     /locus_tag="ACHALG_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFN2586251.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            45270..46913
                     /locus_tag="ACHALG_000823"
     CDS             45270..46913
                     /locus_tag="ACHALG_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFN2586252.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(46986..47531)
                     /locus_tag="ACHALG_000824"
     CDS             complement(46986..47531)
                     /locus_tag="ACHALG_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HFN2586253.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(47554..48039)
                     /locus_tag="ACHALG_000825"
     CDS             complement(47554..48039)
                     /locus_tag="ACHALG_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFN2586254.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(48029..48841)
                     /locus_tag="ACHALG_000826"
     CDS             complement(48029..48841)
                     /locus_tag="ACHALG_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HFN2586255.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(48980..>50091)
                     /locus_tag="ACHALG_000827"
     CDS             complement(48980..>50091)
                     /locus_tag="ACHALG_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFN2586256.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT        16562 a         9115 c         9638 g        14776 t
ORIGIN      
        1 tgttcaatca aaacaagcgg taaagaagca ttgctagaag cttcccccgt tagaaaagat
       61 aggctgaaac ttagaactaa aacgactaaa atgtcatcta ccacagaagc acccaaaatt
      121 gttgatcctt cttttgtatt cactacgttt agttctttta atacttctac tgaaatactg
      181 acagaagttg ccgcaagaat aatcccaaag aaaatcgctt catttcccga aacttggaat
      241 gctttgcctg tcaaccagcc aaaaacaacc gggaagatga tcccaagcaa agcaacgaac
      301 ataccaggtc ggaaatattt tttcaaaaga gataaatcac tttccagacc agctaaaaac
      361 attaataaga acacaccaat ctccgaaaag tcatggacta gaatatcagg tttgacccaa
      421 ttcaatccag cttggcctag caaaacacca acaagtaatt gtccaatcac tgctgggata
      481 cctaatctcc gcgagatatg tgagccaatc gttgttgaaa tcaatatcag gcataaaata
      541 ccaataaatt ccattctcat cattcctttg tataaaatat agcccgaact tagcataaca
      601 tacattattg aaaaaatagg aaaatatact cacaaaaaaa ccaacaatcc aaaagatttt
      661 tttggattgt tggtttttat acggtctatt ttaataacta aaaatgatta acgtatagtc
      721 atttttctta ttctgatgaa attgataatc tcctggatgc ccatgaaaat caaaataaag
      781 ccgaatattt ggaatagaag catcaaactt ttgaatggat tgaacaataa gacgaatccg
      841 ccaatcaaca acagaatact actataaaca ctccaacttt tagcttttgt attcatgctg
      901 aagaacaact ggaacaagct gttgatgata acagctaatc caagaagaat cggcaggata
      961 gaaacgattg caggtgcaaa agcatagacg atcagagcta agatcaggaa aaaaagacca
     1021 ctgaacagac cgatactcca agaacctgtt ttccttttaa aacggtaagc ctcaaaaaga
     1081 ttcaacaacc caagcaaaac aagataggcg gtgatcagat aaccgataaa atgaaaaaca
     1141 gcagaagggt ttagcacaat cgcaatccca gaaataatat aaacagcagc tcttaataag
     1201 gcgtattttt gaatattttg tttaatcgtc tcaaacatat aagaaaacct cctttgactt
     1261 attttaacgt gaactagtca ttttaccaaa ttaaatgtta caaaatgagc gaaggagaag
     1321 aatatctctg gaaaaatcaa cttctgagga tcttttccat ttttttgcct tttgctagct
     1381 catcgatcat tttatctaaa taacgaattt ctttcatcaa gttatcctcg atgtcttcta
     1441 ctcgtacacc acaaacaaca cctgtaatca aggaacgtga aggattgggg acaggggctt
     1501 ccgcaaaaaa ttgttcgaaa gtcacttgtt tttgtagttg atattctagt tctgtttggg
     1561 aataacccgt cagccaacaa atgatttcat ctacttcctc ttttatacga tcttttctct
     1621 ccgcttttgc tacatacata ggataaacgc ttgcaaaact tgtcgtaaaa atacgatttt
     1681 gcgccataat cagtcacctt tctttttttc aaattattta ctacttatag tatataagaa
     1741 aaagagaaga aaaagcgatt attcaaaaaa acaaaaagga ttgtgacaaa attcttgtca
     1801 ccatcctttt ttccgctctc tgacttttta caatcttcat agctcaagtg cgcatcagat
     1861 aatcgaatgc tcctaaagct gcagtagctc ctgaacccat tgaaacaatg atttgtttgt
     1921 atggactatc tgtacaatca ccagctgcga aaacgccggg gatattcgtt gcgccatttt
     1981 tctcggtcac tatttcattt cttggattca tttcgatcgt tccatctagc caatccgtat
     2041 tagggagtaa gccgactaga atgaatactc ctgaaacagc tagtgtatgc atctccatcg
     2101 tacggcgatc gatataggag agagattcta cctgattcgt accgctgatt tctttcgtcg
     2161 ctgcatgttt gataacagtg acattcgcaa gagagactag tttatcctgt aagatttgat
     2221 ctgcttttaa ttctggtaag aattctagaa catatacatg ttttgctaac ccagccaaat
     2281 cgattgctgc ttcaacacca gaattaccac caccgataac agcaatctct tttcctttaa
     2341 ataaagggcc gtcacagtgt gggcaataag caattccttt atttttaaac tctttttcgc
     2401 ctggtacatt gatagaacgc cagtgcgcac ctgtcgcgat gatggcggtt tttgctttaa
     2461 gtgatatacc gttagctaag ccaatattaa taaaatgttg ctctttattt aaggaaacgg
     2521 cctgttgatt catcataatg tcgatagggt aactacgcac atgactttct acttgctgca
     2581 tcaattttgg accttctgtg taagaggtac caatcatatt ttcaatccct agtgtttcaa
     2641 cgacttgtcc gccgaaagaa tcagcgacaa gtcctgtacg gattccttta cgtgctgcat
     2701 agatagctgc actactagct gctggtccac cgccaatcac taatacatca taaggttctt
     2761 tttcaaacaa ctcttcttca atcaaagggc cagtgatttt ttcaacaatt tgttcgatcg
     2821 tcatgcgtcc gctactaaat tcttctccat ttaaaaacac agttggcact gccatgattt
     2881 ttttatcatc gatttctttt tggaacatcc caccttcaat catcgtatga gtaatatttg
     2941 gatttaatac agctaatata ttcaatgcct gtacaacgtc ggggcaatta tgacaagtca
     3001 agctaacata actgtcaaaa tgcaatgttt gtgttatttt tttgatttta agactcaaag
     3061 attcactgat tttcggtgcc cgtccactaa tttgtaacat agccagtaaa aaagaggaaa
     3121 attcatgacc gagggggata ccagcaaaaa cgattccact ttctttgcca actggattga
     3181 tagaaaaact aggtgtgcgc atcgaatctc ttttttccca agagatttta ggagacattt
     3241 ctgctatttc cttcgtaaat tcagctagtt tttgactatt ttcactatta tccaaactaa
     3301 cagaaaaaac aataggagat tcaatcagtg ctaaatattg ttcgagattt tgtttagttt
     3361 ctttgtctaa catatccacc acctaaatct ttccaacaag atcaaaactt ggtttcaatg
     3421 tttcgccact ttctttccat tttgccggac aaacttcacc tgggtgggta cgtacatatt
     3481 gagcagcgcg aatcttatca atcagtgcac tagcgtcacg accgattcca tctgcattga
     3541 tttccatagc ttgaacgatt ccgtctggat caataataaa tgttcctcgc tgagctaatc
     3601 cttgctcttc atccaaaaca tcaaaaatac gagaaatctg atgagacgga tcaccgatca
     3661 tcacgtattc gattttaccg atagcatctg atgtatcatg ccatgctttg tgagtaaagt
     3721 gggtatcggt agagcaagaa aagacttcaa cacctaaaga tttcaatgta ctgtattgtt
     3781 cttgtaagtc ttctaattca gtaggacaaa caaaagtaaa atcagctgga tagaagcaaa
     3841 gaatactcca gttccccata agatcttttt cactgacttc taaaaattct ccttgatgat
     3901 aagcatgtgc attaaattct tcgatttttt tcccaatcaa tgacatagta aaacctccaa
     3961 taataattat ttatttataa ttattgtaat ttagttggag ttgcaaagtc aatggatatt
     4021 ttgttattta ataaagatta ctcaaaaacg aaggttaatt ataaaaatag aagaggtcgc
     4081 aagaaaatgc tactgctgga aatactcggg ataacgttga gtaacagttt cctgttcttt
     4141 cagcatcatg tgtaagtgca atcccgtttg aaatcgagct tcttctggtt ttttatcaac
     4201 taaagccttg agtaaatcat aatgttgttc catgatcgtt tcccatggaa gcccctgaat
     4261 ggtcactcgt aaccagcgga aacgttctaa atgggtatta tggtcatcta accagttcca
     4321 aacttctttt cttcctgcaa tttcgaaaca agcttcatga aataaattat ctgcacggaa
     4381 aaaagcgcgt tcgtcattta ttttgactgc tttttcttgt tcttctaaaa tcgtttctag
     4441 cactattcgt gtttgtgtgt ccaatttgga acaacattca atcatgattt gctcttccaa
     4501 ctgttctcgg acaaagcgag cattttctgc agaaacaaga ttaattttgg aaacatacgt
     4561 accactttga ggtatcgtat acacgagttc ttgctgacgt agttgaatca atgcttcgcg
     4621 aatgggagtc cggccgatag caagatgttc ctccagtgcc ttttcagata ttttccttcc
     4681 aggttctaag tcagcataga taatttgttt acgaatcgct tgataagctt gtgattgcaa
     4741 gctgcttttc atctttattc ctccaaaatc atttctatga acgtagtata acagaagagg
     4801 tctgaaaaac aatctggtat gtcacattaa ctagtaaatt tgtataaata gtaaaatttt
     4861 ggttgacata gcgattttta ttgcataaaa ttcacttgaa tacaaaaact aatatgttag
     4921 tttaggaggg gaaaaatgat tgatttaaac aactggagga actttatccc tgaattatct
     4981 caaaaagaag tagaaatacc tcattttgac cgtgcatcat taagagaaaa tggacaaaaa
     5041 aatcctgtct ggatccattt cggtggcgga aatctttatc gaggatttca tgcagaaatc
     5101 gctcaacgtt tagcggatct aggcgaattg tccactggtg tcattgtttg tgaaacattc
     5161 gatgaagaaa taattgaaag ggcttaccaa gattttgatg atttgattct tgaagtggtc
     5221 atgccaagca gtggagagtt cgtcaaacgt atattagctg cgacagctga cagtctgtat
     5281 tgtcatccag attgttctga acactttgat aagatgattc gacactttga aaatcctagt
     5341 ttacaattag ttacttttgc tattactgaa aaaggttatc gattgaaaga tacaaataat
     5401 aaatatatag gaccgattgc agtcgatata aaaaatggtc caaagaagcc atcacataca
     5461 atgagtattc ttgctgtgct actttataaa cgttttttat ctggagcttg gccgattgca
     5521 cttgtctcta cagataattt ttcacaaaac ggtagtcaat tacgtacttc tttgctaacg
     5581 atcgcaaaag aatggcaaaa ggaaggttat gtacaaaata catttatcca atacttatcg
     5641 aatgaagaaa ttgttgcgtt tccatggacg atgattgatc gtatcacccc gaatccttcc
     5701 ttgtcagtta gcaaaatgtt ggcaaaagag ggcttttcaa atatgtcgat tttccaaacc
     5761 gcaaaaggaa ataatattgc tccatttgtc aatacagagg acatccatta tttaatcatg
     5821 gaagatcatt ttcccaatgg acgtccgaaa ttcgaaaaag ctggggtatt cttgactgat
     5881 cgtgcaacgg tagataaagc agatacgatg aaggtgacga cttgccttaa tcctttgcat
     5941 acgtctttag ctattatggg ttgtttactg gggtatagga gcatagctga ggagatgaaa
     6001 gatccagatt tatcgctgct agtttctgaa atcgggtaca atgaaggcct cccagttgtg
     6061 gagtctccag gtatcattga tccaaagatc tttctggaag aagtaattac tgtgcgcttt
     6121 tctaatccaa atattcctaa tatgcctcag cgaatcgcca cggatacctc tcaaaaggtg
     6181 gcgattcgtt atggtgagac gattaaaaaa tatatggaaa tagaagttaa agacccgcag
     6241 acacttgtat tcataccatt gactctcgct gcttggcttc gataccttat tggcattgat
     6301 gatcaaggaa aaccattcat cccgagtcca gacccactat tgacagacct tcagcataag
     6361 ctttccgata ttcaactggg tacgcaagat acatcacata ttcatgagaa aatggctcct
     6421 attttgtcaa ataaagaaat ttttggtgta gatctttacc aagcagagct tggcagaaaa
     6481 atagaagaaa tgttcatcaa gatggtagca ggaccaaatg ccgtacgaaa aacattacag
     6541 gattacttac aagaacatgg aggaaattta tcatggaaat gacttttaga tggtacggat
     6601 cacaggaaga aaagattacc ttagatgaaa tcagacagat tcctgcggtc aaagggattg
     6661 taggcacatt gatggatata ccagtaggag aggtatggcc tagggagagg atcaaagaac
     6721 taaaagaaga aattgagtct cgtgggttaa cactaaaagt aattgaaagc gtcaatgtgc
     6781 atgatgccat taaaattgga ttaccttccc gtgagcagta tatcgaaaac tataagcaga
     6841 caatccgaaa cttggcagaa tatggaatag aagtcatctg ctataatttt atgcctgtat
     6901 ttgactgggt gaaatctgac cttgactacc gcttagaaga cggctcatcc acgttagctt
     6961 ttatatctga tgatattcca gatgatccaa gagaaatcgt cgcgcgcatc gaacattcca
     7021 gcaatgaatt tggattgccg ggatgggaac ctgagcgctt agctcctatc aaatccttgc
     7081 ttgaagccta tgcatcagta gacgaagaaa aacttcgaga aaattttgcc tattttctcc
     7141 aatcaatcat cccaacatgt gaagaggtag gggtcaaaat ggctgtacat ccagatgatc
     7201 cgccatactc gatttttgga ttagctcgaa tcgtaaagaa tcgagaagat ttagattggt
     7261 tgtgtcgcat agtagacagc ccttataacg gaatcactct ttgtacaggg agtattgcag
     7321 aagatccaga aaatgatgta tatgggattt tagcagaatt ttgccaaaga gatcgaatcc
     7381 catttgcaca tgttcgaaat attaaatttt tagctaatcg agatttctat gaggcaccac
     7441 ataaatccga atacggttca ttagacatgt accgcatact aaaaacaatg tatgacaacg
     7501 gatttaacgg atacatccgt cctgatcatg gaagaatgat ctggaatgag acaggaagac
     7561 caggatacgg cttatatgat cgagcattag gtgctgttta tctaaatgga ttatgggaag
     7621 caatcgaaaa aggaaacaaa agtaaagata attagtgaaa aaaactgtaa ttttattatg
     7681 taaataagaa ggatgttcaa attcttgata cataaatccg gaggttgtta ataagcattc
     7741 ggatttatgt atcttatttg gtctaggaag taacttgatt ttcttttttc gattgatcca
     7801 tgagtgcttc ttcttttaat tcaaagatat attctgagat cctcataatc acttttttcc
     7861 agtcttctgg tgtgatgatt ccttttaaaa tatagatttt tttgatatga gaaggcaatt
     7921 gtttcaaagg gcagtcgctg ataacgatgt tgcagttctc cataaaatca ttttgtattt
     7981 tgatcgattc atttgaaaaa atttgttcta tcttaacgat cagatcttta ctgatataaa
     8041 aatcacgcgt atagttcaca ccaatcacaa ttcgcggctg ttttttgtag cgatcataca
     8101 aatgtactag gtcttcaacc atccattgca cattttcctt ttctaaaata actggtaaca
     8161 agtgttgatt ttgttctctg aaatccatat aaaattgttc aatggcggtt tggacttctt
     8221 tcaatacgct atgttcttga taaagcatga aagtaggaag gggcgtattt ggttgattga
     8281 agttaagaaa tccattataa agtttatttc gtgcgaatgc caatgagtaa agaatctttt
     8341 ctttttcagg tataaacaaa tcaaatacag tacaaaggga ctccaccatt tctcttgaat
     8401 aaagaacatg aggaacgttt gcttttttta aaagtttgta ttgctccaag cttgtttctt
     8461 ccgattcgat tttcgtgata gaaagtcgag ctaaaatatt cactaagatg cgttgttcgc
     8521 gacaaattgg cagattaggt cgcaaaatat ctacagagag atcaataaaa aagtcaatat
     8581 attcatactc tgtgaattct ttttgtacgt ctttcacaga agttgcagga aaattctctt
     8641 tacatatttt taatagaaaa tataatttat ctatcatacc gctatttagt cggtcaagta
     8701 cttctttttt aaaaaaagta ttcatcataa aagccactac ttgcggatct tcatcataaa
     8761 attctgtagc agtacttgaa aagatgttcc aataaatatc taatgctgca tattgaattt
     8821 gtacctcttt tccagaaaaa gactttctgc cattattaga aaaggagata gacaaaccgt
     8881 actatgctaa aaatgcatta atctttttca ttttcgtata caaataagag gcactatagt
     8941 ttgtttgctg actgaaactg ataatcgaat ttgtctgaca gctgaacaaa gaagtcaaga
     9001 tctgataact gactgattcg ttgcagtatc ttccaaatag acgataatag cagtaagccg
     9061 gatcaattcc ttcaaaattc aactttaaaa aaggtttttg cttatcaata tgcagttctg
     9121 ttagattgag acgctcaaag tcttgttcta attctttgat gtatagtaaa aaatttgatt
     9181 cagacatatg gaaatgatct gctaaatatt gtgtagtaat aaagaaacga tgttcagata
     9241 ggaaaagaaa aatatctaat tttgtattgc tttttttctt caagataaac gttttaatcg
     9301 cttcttgagt ttgcatgaaa gaggctcctt tttacttaaa ttgttaaaac ttgaaaatat
     9361 tactgttttt ttcataaatt tagacaaaaa aacagtaaac atataggtag aatctctact
     9421 atcttatttc ttgcatacaa aaaaatcaag gagtgagaga gttatgagat attcaaccat
     9481 cttaatatca acatttgcag ccttaagctt atcgcttact ataaatcaga cagtccacac
     9541 agaggagaca gaaacagcca aatggacaga aaccaagtgg gccaacgtca atcgaattac
     9601 ctttcaggat gaattttcat ccttcaattc aggagtcgca aaattaagaa aaggacaaac
     9661 agcgaccatc aaagcaaaaa ttgactattc gggagactta gcacctatac tacaatcagc
     9721 tgttacattt gagaatgttg atcccacttt gtcgattggc tatgacgaga acactttgac
     9781 tatccaacac aagcaagcga tatttacctt caccgttaca ttaaatcagg acttgttaaa
     9841 accagcgctt tttactgtca aggtatcaga tggactaccg aacgatcatc atttaacgcc
     9901 gtatagtcag cggcagacaa ttgagcaaga ttcagccatc gactcaggag gagatcttgt
     9961 tgaagaacca acagataaac cagaaaatga aaataagcca gaagtaccgc ctacagaaaa
    10021 tccagacgga gaacagaaac cggaaataga acctggagaa gaacccgata cagaaactca
    10081 accagagcca gacaataaat caaaacctga aataacccct ggagaaaaac cagatgtaac
    10141 tccagaacca gatacggatt ctggaaacca aacagtaccg gaaacaaatc cagacacaga
    10201 taatgaaact gaaaatccag aaaaaccaga ggtagatcca gaagaaaaac cagatgtgac
    10261 tccagaacca gatacggacg caagagacca aggaatacca gaaaaaataa acaaaaaaac
    10321 aattcaagag gatggaaaaa aagagagtaa aaaatcaaat ctagcaattc taaaaataaa
    10381 tgaagagcag ctgaataaaa aatcacgaat atttgatagc gcccaatcag ctgaaacctt
    10441 aaaaagtagc aaggatacaa cctttgctgt cctgaaacga aaaataaaca gctaccaaaa
    10501 tccggagagt cacaaaacaa agttatactg tggagcggaa tcattctatt aagtatagca
    10561 acaatgcttt ctgcaaaaag atttaaacaa aacagatctc tctaacattt gaataatgtc
    10621 tgtgaacgca tttttcaaat aaaaagaaat atccagaaaa tgaccccaaa ataccagatt
    10681 gaaatctgat atttggggtc gttcatactt tatctttctt catatttatt ttaagggata
    10741 tctgtttctt ttggaagatg gttcgggatc tttttcactt tcaatacatc taaaaagaag
    10801 gtaaagatcg gaatgccgac aatcaagccc caaacgccaa agaaccgttc gctaacaagt
    10861 aagaccacaa atgtataaaa aatcggcagt tctgttttgc tggacatcaa cttcggattc
    10921 agcacataag actcaattag atggacaatc aaaatcgtaa tcaggatata aacaacatct
    10981 cggatgcctc cttgggaata agctatgaaa gatagaggaa tacaagaaat gataacacct
    11041 gctactggga tcagacttaa aatgaaaatc atgatcgcta aactcagcag ctgatgaaag
    11101 ccaaatacag ccaaggcgat tgtagtgagc aacgtattta aaagagcgac aacaaattga
    11161 gcttctaata ccaaaccaaa tgtattgaca aacttatcgg caaaatagta aatatcttga
    11221 aagaaccaag aaaattcact ttttaaaaac aatcttgaga atacaattgt ttttttcttt
    11281 tcaatcataa aaaagaaact taagatgaat gacatagcaa acgccactgc catttttcct
    11341 atatcctgta aatagccaag cgcaatcgaa gcgccatttt gaagctgttc aaaaaaatcg
    11401 tttcgctcaa tataagtatg gatatattgt aaaacaggat ctgcatcttt tggctgatgc
    11461 tcgtaaaaat taataacgga ctggatcatg tcaaatgttt gatgaataag catcgggata
    11521 tactttgtga atgccagata gataaggtaa acgatcagtg tataaatcat gatagctaaa
    11581 acacctgtag ggattcggac aaatcgctga atgatttgaa caagatgaat catcaaataa
    11641 gtaaagataa atgtgagcaa aatggttgta atcatacttc taactaagaa aagaacaaaa
    11701 acgacaaata ataaaacagt gagacggcgt attttttcat tcgccaaaaa acgctgataa
    11761 acggtcaaat cgacaacctc caaataacaa aaaataaaca cgttattcat tatatgatgg
    11821 aatatgaaaa taagaaagag caaaaagaaa aaccgctaaa tgacgttatc ttatgagttg
    11881 gttttcctca taaaatcgtt catctagcag tttcagccct ttttctggtc ttgatgaaaa
    11941 ttatctagca tttttcgggt cagatgttgc agtgtatcca attcttcctg ttcaagtgga
    12001 agataagact tgatcttttg tggaatacct tctgcttgtt tttgcaacgc agcaccttga
    12061 gcagtcagcg taacatttac gattctttca tcacccgttt cacgctgacg attaatataa
    12121 tttttttctg ctaacttttt tagtaaggga gtcagcgtac cagaatccag acacaaatca
    12181 tttcctagct ctttcactga agtctttttc ttttcccata aaactaacat cacaagatac
    12241 tgggtatagg taagattcag cggcttgagg aggggagtgt agtgtctaat gatttcttta
    12301 gaggcggcat ataaaggaaa acatacttga ttaactaaat atagatcttc catttttcca
    12361 cctccgctgt tttactatag tgtatcatag cggatttaca caaaaaagtc tattcactaa
    12421 ctgctttcag agtaacttct atatttccag ctgtagcttt tgaatacgga cacactttat
    12481 gcgctgcctc taataattct tgcgtttctt ccagagaaag accttcgata gatccttcaa
    12541 tctctacacc taagacaaag ctcgtttcac tttctgaata aagggaaact tgtgcactga
    12601 cagtagaagc cccagagata tttttttgct gtttgatcaa ttctaatgca ctgttaaaac
    12661 atgcactgaa accagctgca aacaattgtt cagggttggt tgcattttcg acgcgtttac
    12721 caggagctgc aatattcaat tcgaatgtac gatcgggatt atgcacttct ccagagcgtc
    12781 cgcctgtgtt gatcattttc gtagaataca tttttttcat aaagttacca cctttaatta
    12841 tattttatac aattgaattt tacacaatat aattgtggaa agcaaagaaa tggattggca
    12901 taagataaat aagagaaagc acatgaaaca gacgcgcttt ctcgaatagc aaacatatac
    12961 aaaaaatatc agagacaaaa aataatctca tcataaacat tacatatatt tctcaaaaag
    13021 ttgttttctt tcttcaaaaa ttacttcacg atggacctga tgaagcatcc ataaataacc
    13081 aaaaggatct ctgaacattg agttagtcac accaaagtcc atcatttctg tcatttcttg
    13141 aatctctaca cagccatttc gtaatgcttt attatgtgtg tcctcgatat cagagacgat
    13201 gatattgaac cagatcgtat ttggatgttc agcagtagga gcagtcaaac caagagacgt
    13261 gttttcatcc agcatatgga atctcgtacc atacaaggta aaaatgactt cattattgcc
    13321 tctagggtaa tctgttgcct caatcagttg tatttcatca aagattgttt gatacagttc
    13381 tagtgctttc aaactatcat ttaatacaaa atcgatttcc gttcctttat gcataaagat
    13441 ctcctctcct tttcagtcga acagttacta gattctttta ttataccagc taaagaaaac
    13501 gcattcaaaa aaatagcgga tttacttacc aactcattcg attggatgat aagtaaatcc
    13561 gcgtcccatt acttctgcta cgctgctgac aatgacaaag gcacttggat cgatttcttc
    13621 tatcaatggt ttcattcggg aaaattcatg atcagtcaca acgatcaata aaacctcttt
    13681 tgactttcga ttatacccgc cttttgcctc tagcagggta aggccacgtc caatctcagc
    13741 acttaagcga aggcggatgt cttccaaata atgttcactc atgaccataa cagatttctt
    13801 tcgattgata cctgtttcga tataagtcat gacaattgaa gtgattgcaa tagataaaat
    13861 agcataaaga aactcttcta ttccaaaaat aaacagatta agagaaacaa cgaatgcgtc
    13921 tgtaaaaaat aagccaatcg aagtattcaa gtggaaatat tttttaaaaa tgaggggagg
    13981 gatcgtcgta ccgccgctag aagagttgtt tttatataaa atcgcaactc ctaaagcaaa
    14041 aatcgcactt ccaaaaatga cagacaacaa ccgatctgaa gtaatcattg tttctggaac
    14101 tattgcaatg attaaaggaa aaaccaagct tccgaaaagt actttaaaaa agggtttttt
    14161 acctaaaaat agaagagcta gtaccaacat aacgatattc aacactaaaa taactatagc
    14221 tcggtcaaag cctaaagcag cttccagtaa tattcctaga ccgctgacac ctccagcagc
    14281 aatatgatga ggccctaaaa acatattgat agcgactcct aatatgaaga gggcaaaaat
    14341 aatcttaatg tatgtccata taaattgtct cgtttctttc atttattcac ttccttaatt
    14401 cattaataat actcttatct tattataact tacttttcac cttaagttaa ctttaattaa
    14461 taaaaaaatt aggctagtct aaattattat gtttcattct tctagaatat acttctacgc
    14521 caaaaactgt taaaaaactt ttactttaga aaattggaga aattgatcgc ttaagataaa
    14581 ttcaggacac taaatagtac aggaagtaga ctaaaacttt taaaagtttt aagtctatct
    14641 atttggcaag tttaaaaaat aaaaattcgg tatcaaaaaa ctgttaccga taacattttt
    14701 gaaagcgttg tcttttgctt ctctatcact tatagtgatg acgtcatatt aaaacacaag
    14761 gaggaaatca atggatacag ctgcaattta tcatcgaccg gaaagtgaat atgcatttct
    14821 atatacgaat acaagatttc gtattcggct tcgtactagg aaaaatgata tcaaaaaagt
    14881 atatgtgcta tgtggagatc catataccat aacaactgaa aaatggtatc aaaaacaacg
    14941 accaatgaaa aaatgtctaa gtaccaatgt tcatgattat tgggaaattg aagtgacgag
    15001 cgaaacaagt cgcttacagt atgcgttcca tgttgttggg gaagataaca cggactgttt
    15061 ttacggtgat caaggaattt ttccttatcg agaaaatgtg attactgaac caaattttta
    15121 ttttcgaata ccttattttc atcaaatcga tcgattcact attccagaat gggtaaaaga
    15181 aacagtatgg tatcaaattt ttcccgaaag atttgcaaac ggggacaaag caaatgaccc
    15241 cgaacacact ttaccttggg gaagtaaaga tccagaccga gaagactttt tcgggggaga
    15301 tttgcaaggt gtgatggatc atttggatta cttgacggac cttggcatca atggcatcta
    15361 tttttgtccc atatttaaag ccacatccaa tcataaatac gatacgattg attattatga
    15421 ggtggatccc gcttttggcg ataaaaaact tttaaaaaac ttgattgatg aagcacataa
    15481 aagagggata cgtatcatgt tcgatgctgt attcaatcat atgggtgtcc attcaccaca
    15541 gtggcaagac gttctcaaaa acggagaaaa atcgatctat aaagactggt tccacatccg
    15601 ttttttccca gtagattctt atcagatgac tgacttacca gaaactgctg aaaatatccc
    15661 atatgatact tttgctttta ctccattcat gccgaaacta aatacagcaa atccagaagt
    15721 gcaaaaatat ttgctggata tcgcaactta ttggataaaa gaattcgata ttgatggatg
    15781 gcgtcttgat gttgctaatg aagtcgatca tcatttttgg aaaaaattca gagaagcagt
    15841 tacagaaatc aaaccagaca tctatatcct tggagagatt tggcattcct ctcaagcttg
    15901 gctgcaaggg gatgaattcc atgcagtgat gaattatgca tttacagatt cgattaaaga
    15961 ttattttgca aaaaagaaaa tcacagcttc acaaatggtc agtggaatga atcatcagca
    16021 aatgctttac agagatcagg tgaacgaagg aacattcaac ttattagatt ctcatgatac
    16081 tgccaggatc ttgacccttt gccaaggtaa caaagaattg atgaagtctg tgatggcctt
    16141 tatgtttctt caaaaagggt ctccttgtat ctattacggg acagaaatag ggatgactgg
    16201 tgaagatgac ccggattgcc gcaaatgtat gatatgggag aaggaggaac aagatctaga
    16261 gctattcggt tttataaaag aacttgtttc tctaaggaaa cagttgtcta agattatttc
    16321 tgaaggttct actcagtggc taatagtaaa tgatcgtgaa gataagctct atttcacacg
    16381 gaaactagag ggacagatca tctatgttta ctttaatcag tcaaaagaac cttgggtagt
    16441 agaacaggaa aatgaggtga ttcttagtca aaattgtcag ttgttagaag acgggaaagc
    16501 agagatacga caatacggat ttttgatcat gtgttgaaaa ctgttaccgg taacaacaag
    16561 aaagcgtttg caaatgaaga tttgtgccca ttataataag ggtgtaacag cacgttgaat
    16621 acaaaggagg aaatgaagat gaaaaaaaga agtgtgaaac agttgggttt agggttaatc
    16681 tctttaggaa tcttaggcgg attagcggct tgtggcaatg gtaactcttc agcagaaggg
    16741 gaagccgatg aaggaaaaac gttaacagtt tccgttgatt ctggatataa agattatata
    16801 aacgaaataa aaaacacttt cgaaaaagaa aacgatgtga aaatcaagct agtagaaaag
    16861 gacatgttcg atcagttgga atcattagcg ttagatgggc ctgctggaaa gggacctgac
    16921 gttatgatgg cagcatatga ccggatcggt gcgttaggac agcaaggaca tctggcggaa
    16981 gtaaaactgg gaaatgaagc agcttacgat gaaacagaca aagcccaagt gacttatgat
    17041 ggaaaaatct acggagaacc tgcagtaatc gagacccttg ttttgtacta taacaaagat
    17101 ttagtcgata cagcaccatc aacgtttaaa gatttggaga atctatccaa agacagtcgt
    17161 tttgcttttg agtcagaagc aggaaaaaat acaggatttt tagctaaatg gactgacttt
    17221 tattattcct atggtttaat tgcaggttac ggtggctatg tatttggtga tgatggcaca
    17281 aatccttcag atatcggttt gaataatgca ggtgctgtag aaggaatctc ttatgcaaca
    17341 gattggttca aaaatgtttg gccaaaggga atgcaagaca taaaaagtgc aggagacttc
    17401 gccagccagc aattcatgtc caataaaact gcagcaatca ttgacggtcc atggcaagcg
    17461 caaacataca aagaaaacaa tatcaattat ggtgtagcca aaattcctac tctaaataat
    17521 ggacagcctt atcaaccgtt tggcggagga aaaggatggg tagtcagcaa ttatgctaaa
    17581 aacaaagacc tctctcaaaa atggcttgat tatgtaacga atcaggaaaa tcaagaaaaa
    17641 ttctttgaaa tggtaaatga aatccctgca aaccaacaag ctagggaaac agcaaaggct
    17701 aaaaacgatg aattaacaac agctgtgatt gaacaatatg aaacagctca agcgatgcca
    17761 aacatcccag aaatgggcga agtttggaca ggtgcagaaa acttgatgtt cgatgcagca
    17821 tcaggttcaa aaacaccaaa agaatccgca gatgaagcag taaaaacaat cagtgaagcc
    17881 atagaacaaa aatactgatt aaaatgataa ccgtccattt ttataaaaaa tggaacaggt
    17941 gcttcagata aaagtggcaa gagcgagatg gaaaattatc ttgctcttgt tgccattatc
    18001 ttaaaagcag tttgcggttt agcaagttta gagggggcat tcagatgcag caagataaaa
    18061 caagcaatat caacgaaaaa aaaatcacgc tttgttcact tattccaggg atggggcagt
    18121 ttgtaaatgg ccaagctttt aaaggtatcg tttttttcgc catattcgtc ctattcattg
    18181 gtcagatgct tctgggcgga tttcatgcat tggtaggatt ggttactttg ggaagtatac
    18241 caatggaaga tcattcatta tttattttga ttgaaggcac tttgcaattc atcattacaa
    18301 tcattttctt gattttttac ggtttgaatc ttttagacgc ttatcgagta gcgaagatgc
    18361 gtcaagaggg caaaaaaatc aatcggacga tgaaggaagt catacataat ataggtgatg
    18421 aaggttttcc ttatctattg acacttcccg cttatcttct catgattttt acgatcattt
    18481 tcccagtcct agttacattg ttcactgcat ttactaatta tgacttcaat catattccac
    18541 cagccagttt aattgattgg gtaggggcta aaaacttttt tagtatcttc tttttaagct
    18601 catatcgtaa tacttttttt gcagtctttt cgtggaccgt gatttggact ttatgtgcaa
    18661 caactttgca aatcaccctt ggtattctca cagcggttgt tgctcaccag aaatttatta
    18721 aattcaaacg tattttcggt atcattttct tattaccatg ggcggtccca gcttttatta
    18781 ctatcatgag tttttcgaat atattcaacg atagcgtagg tgccatcaat actcaagtga
    18841 ttcctttcat aaaccagctt ccttttgtag atatcagtgc aatctcttgg aaaacggacc
    18901 cgaactggac gaaagtagca attatcttag tgcagggctg gttaggattt ccctatatct
    18961 atgtgatggt tacaggaatc ttacaatcga tttcagaaga cttatatgaa gcagcaaaaa
    19021 tcgacggagc aaatgcatgg cagcgctttt caaacatcac gcttcctgct gtcttcctag
    19081 tggcagcgcc aacatttatt acacagtata cggggaattt caataatttc tccatgatct
    19141 atctgtttaa caatggcggt cccggaagcg taggcggagg agctggttct actgatattc
    19201 ttatttcctg gatctataaa cttacgactg gaacttctcc gcagtattct atggcttcag
    19261 caatcacgtt gattatctcc gtcatcgtta tctgtgtgtc attgctggta tttaaaaaga
    19321 caaatgcgtt caatatgggg gattaacaat gaaaaaattt cattcgagtg ctcgattcca
    19381 aaaacgactt tctcactttt ttacttactt ttttctttta atactgtcag tcattatcat
    19441 ctatccgctt ttgatcacag caagtactgc cttcaagtca ggaaatattg cagcttttag
    19501 tttagatctt tcaagtgact ggactttagg aaactttact cggctattca gtgaaacact
    19561 ttacgggact tggtataaaa atacattgat catttctgtc ctaacaatga tcgtacaagt
    19621 gactgtagta actttggcgg gctatactta tagtcgctat cgttttatcg gccgcaaatc
    19681 aagtttgatg ttctttttag tgattcagat ggtaccgact atggcagcat tgactgcctt
    19741 ttatgtcatg gctttcttat tagaagcgct cgataagtac tggttcttga caatgatcta
    19801 tataggcggt ggaattccaa tgaatacttg gctgatgaaa ggatactttg acactgtacc
    19861 aatagattta gatgaatctg ctaaacttga tggggcaggg catttcagga tttttttcca
    19921 gattgtatta ccgcttgtca aaccgatgct cgccgttcaa gcattatggg cttttatggg
    19981 accttttggg gatttcatgt tagctaagtt cctgcttcga acacctgaaa atcagactgt
    20041 tgctgtcggc ttacaaacat ttatttctga tgtgacaaac caaagagtct ctctttttgc
    20101 tgctggggca attttgattg cactaccgat ttgtctatta ttcttctttt tacaaaagaa
    20161 ctttgttacc ggcctgacat ctggcggaac aaaaggatag gagcagagta tcatgcaaac
    20221 atttccgtta aactattttt ccgcattacg tacgccaact caagtgtttg cgggacgcaa
    20281 actattatca tggcctaaat ttttcttgat atttgtattt ttagtttcct tgatggtcat
    20341 gcctgtgact cttttttatg ccaatcagat ccaagcgatt ccattagaac aatttctatc
    20401 cgttcattcg ctgatcgatg aacaaggaac ccaaaaattt tctgaacttg aattatctga
    20461 aactgggtta caagcatcgc aacaaacgat agctgtgacc cctgaaattt tggtaggtgt
    20521 gtctttatct gagaaacaac aatccgatca tggaactttt attgattttg agaaggaaca
    20581 atgggtgatc caacaaaagg ataaatctgg tatacgccgc tatacgatga attatagccc
    20641 atctttccaa ccagattctg tacgtacgcc cgaagatttc caacgatttt tagaaagaga
    20701 attttatgcc agtaatcgtc caacaatcat tttatcttat agtttaagtc ttggtcttgt
    20761 cctatttgtc atgacgagtc tcattttatt cggggcttcc tttttcttgt ggatgacaag
    20821 aaaaagccaa ctttcatcca ttcatacatt caaagagagt gcaaatttga tgctgaatgt
    20881 tatgggaata ggttccatgg cagcagcagt tgtagggttt atccactttg actttatctt
    20941 gatgctaagt gtccaaacaa cgataactgt cttgctgctt ttatgggttt ttgtcaaaac
    21001 aagattcaaa gataagcgtg tcgaatgaca agtttaagcg atttatgcga caatgaatgt
    21061 gaaaaaaatg aaaaataaag tagtttttga aaaatttctg aaaaaattaa acttcttagt
    21121 tgattctttt cttttttagt attaacgtaa tagggtagtt tttttaagga ggaaaatcat
    21181 gttagagaaa actttataca atcaattatt ttcacgttct ttctcgctcc ctgtcgaagt
    21241 aaattattgg gacggtacaa cgaaacaata cggagatacg gacactcctc cccaaatcaa
    21301 aatcaatatc catgaagaaa tcccaatgaa agagattaca agtaacgcat ctttggcact
    21361 aggagaagcc tatatggatc atcgaatcga gatcgaggga agtatccaag cattgatcaa
    21421 cgatgcttat cagcaagcaa acagcttttt acgaggaaaa gattatctaa aatggctgcc
    21481 aaagaaagaa aaacatacga aacaaaaaaa caaagaagat atccatgcgc attatgactt
    21541 aggtaacgac ttttataaac tttggctaga tccaactttg acttattctt gtgcttattt
    21601 caatacacct gaagatacgt tggaacaagc acaaatcaat aaagtccatc atatcttaga
    21661 caaactcttt atcaaagaag gcgaaacgtt gcttgatatc ggttgtggct ggggaacatt
    21721 gatgttcact gctaccaaag agtataatgt caaggcaact gggatcacac taagtgaaga
    21781 acaatacgat ttgattacca aaagaataaa agaagaacat ttagaagata aatgccgtgt
    21841 attattaatg gattaccgcg aactaaaagg cgaaacgttt gatcatatca caagtgtagg
    21901 tatgttcgaa catgtaggtt ctgaaaatct tgaaggttat ttcaaagtgg taaaagattt
    21961 attgaaacca aaaggaacag ctttgatcca tggaatcagc cgacaacaag gcggtgcgac
    22021 aaatgcttgg atcaataaat atattttccc tggcggttat gtccctggtg ttactgaatt
    22081 agttgataat attacaaaaa acgatttgca actgattgat ttggaaagct tacgtcgtga
    22141 ctaccaattg acattagaac attggacaaa gaattttcat caagtccaag atcaagtcac
    22201 gaaagaaatg ggtgattcct tctatcgaat gtgggatcta tatttacaag cttgtgcagc
    22261 ttcatttgaa tcaagtaata tcgatgtgat tcaatatttg cttgttcaac ctggaaacaa
    22321 tgacatccca atgcatcgtg cataattgag gcaaaagtac tgtttaaaga ctatttaaaa
    22381 agaagctaag ttttatgatg ctccagaagt gaactttttt gtctaacttc taggattgtc
    22441 tctaaagctt agtttctttt tctttatatt ttcataataa aagtttggtt ctttaatttt
    22501 taaacatgac agaaaatttt ttcttcgtta tacttgtaat agaatactat tttataacaa
    22561 aggggatcga atcaatgaaa aaaactgtta tttatactag tttatttgca gctttgtttt
    22621 tagctgcttg tcaatcaaat accaatcaag gcacatctgg gaatacgtct tccagtagcg
    22681 aacagcgctc ttcacaggta tcggactcct caacaaaagc aagcacatct tcgtctagcc
    22741 agacaaaaag ttctagtgaa gcaagtactc agtcagatat caaagtgtct gcagctcgtg
    22801 cgatcgaatt gttccaagat aaatacccag atactgcaat tactagtttg gaattagata
    22861 gtgattgggg atcttacttc tacaagatag aaggtgtcga tgatcaaaaa gaatacgaag
    22921 caagaattga tgctcaaaat gaaaaaatgg agacagagaa tccagaaact ctagatcgag
    22981 atgaacaaaa tggtcaaaag aaaaaagaag atggtctgga tgttgataaa ttgatcacga
    23041 ttgaggaagc agcaaaaatc gccgtcgaca aagtaggaag cggtacggca acagattggg
    23101 atttggataa agatttaggt actacttatt gggaagtcaa agtaaaagac ggcaatcaaa
    23161 gtacatcagt caagatcaat agccaaacag gcgaagtact gtcaactgaa caagatgatt
    23221 aagaatttct tcggaaaaca agagttgatt tttcttttag tcatgataca ctagacaaaa
    23281 taacttgttt gaaggagcga tttacttgag tttagaaacg acctttgcca atcatatctt
    23341 tgccaatcct ttgatgaatg catctggtgt ccactgcatg actacccagg aattagatga
    23401 actggctcat tcagaagcag gagcatttat cactaagagc tgtacgatca atgaacgaaa
    23461 aggaaatccc gaaccacgct attttgatgt tcctttaggg agtatcaatt ctatgggact
    23521 tccaaactta ggtttttctt attatctaga gtatgctctt gcgtatgaaa aagtgcagga
    23581 aaaccaaaat caaccgttat ttttttccat tgccgggatg agtgtccagg aaaacttgga
    23641 aatgcttgag aaaatagaaa aaagtgggtt taatgggatc actgaattaa atttatcttg
    23701 tcccaatgtg cctggaaaac cacaactagc ttacgatttt gaggccactt atgaaacact
    23761 gaaagaagta ttcagcatct tttctaagcc gctaggaatc aaattgcctc cttattttga
    23821 ttttgctcat tttgatcaga tggctgatat cttgaatcag tttccattga cctatgtcaa
    23881 tgctatcaac agtgtgggca atggtttgta tatcgataca gaacaagaag cagtagtcat
    23941 caaaccaaaa gaagggttcg gcggcattgg cggagagtat atcaagccga ctgctttggc
    24001 gaatgttcgc gctttttata cacgtttgaa accagaaatc caaatcattg gtacaggtgg
    24061 gattcggaca ggacaagatg catttgaaca tctgctatgt ggcgcaagca tgttacagat
    24121 tggtaccgag cttcataaag aagggccgga aattttttct agaatcataa aagaattaac
    24181 gcagattatg tcagaaaaag gatatacgtc aattgacgaa tttaaaggca aattacgcac
    24241 catttcatag caaagagacg aaggtcatga ttgatcttcg tcttttttta taaaaaacac
    24301 tcgtctgttc attcctgtgc aagcgtactc attgtatgtt agacgaactt gtgggaaaat
    24361 aacagaggca aaaaggagga taatatgaat gatgcaacag aattagcaaa agagcttaaa
    24421 gaaaaagaat ttagttttca agaactagtt acggatatac agaaaaaaat aaagaagcaa
    24481 aatcatgaat tgaatgcgtt tgtcgcgctt gaaccattcg agagtaattc ccacgagcta
    24541 ttgattcccc agtcagaaaa agggccattg gctggtatcc catttccttt aaaaatgctc
    24601 ggtcaagaaa aaaagggatg gctagctact tcaggatctc gtttatttga aacacaccga
    24661 gcttctcata catctaatta tgtgcggcaa gcagaagcga taggtctagt tccttttgga
    24721 caaaccaatg cgccagagtt tggtttcaaa aatattacag acccagtcat ctatggccca
    24781 gcaagaaacc cttggaacct tgaatgtaca ccgggcggat caagtggagg agctgctgct
    24841 gcagtttcat caggaatcgt tcctttagct ggtgctagtg atggcggtgg ttctattaga
    24901 atacccgctt cgttttgcgg attgattggg ctgaaacctt cgcgagggac gatgccagtt
    24961 ggtccatatg cttggagagg atggcaagga gctgctattg atttcggctt gacagtttct
    25021 atgagagaca cggaggcttt attttatggg atgagaagta tccattccgg tgcgccatat
    25081 caagtatcgc cagttgagtg gcaaacacat ccaagaaaaa aacggttgaa gatagctttt
    25141 tgtgttgatt caccgatcca ctcacagatt agtgaagagg cagtacttgc tgtaaaaaaa
    25201 gctgtagatt tccttggaaa agaagggcat gatttgacag agattcctta cccactagat
    25261 ggtcgtaaac tgatcacgtc ttattatcag atgaatggcg cagaaacagc agcaatgatc
    25321 cgctcgattg aaacaggcat ccagcgagct gtcagaaaag aagaacttga acccatttct
    25381 tggactttgt tccaatatgg tcagaagctc tctgccgcta cttacatcga ttctctgcat
    25441 gtatgggatg aagcagcaat tgtcatggaa aacctacatc aagaatacga tctattcctt
    25501 tcaccaacga ctgcatttac cgcccctaaa atcagcgaag atttgcaaag tgatgcgata
    25561 agagcacaga tggaacacat cagtgattgt tctgaagtag aaggtttaga cgttgtttct
    25621 tccatgtttg aggagagctt gaagatcaca ccttatacac agttggctaa tctgactgga
    25681 caaccagcaa tcagcttgcc tactcatttg acaaaagaaa acatgcctct aggaatccaa
    25741 tttatggctg ccagaggaag agaagatttg ctatttcaaa tcggctatct atttgaagca
    25801 aatcatcagt tccatttacc aaatccatgg aaatagcaga aacaaggaga agaagaatga
    25861 agaaaaaaag attgtttgct ctaataacta gtgccttttt tattttaagc gcttgcggta
    25921 cagctgccgc taatcaggaa gataacagct ggagtaatgt agaaaaaaag aaagaattag
    25981 tagtagcgac ttctggcaca ctgtttcccg catcttatta taatgatcaa aatgaattag
    26041 ttggttatga tgtagatttg gcaaaagagg tggctaaacg gttaaatgtc aaaatctcct
    26101 ttaaagaata caacgtggat ggacaaatca cttctgtttc aaaaagagaa tcagattttg
    26161 ctgcgaacga ttttggtatc acaaaagaaa gagaaaaaaa attctccctt tcagagactt
    26221 tcaaatattc atttgacagt atgatcgtac gaaaaaaaga tgattcagga atccactcac
    26281 ttgaagatct taaagggaaa aaagcagctg gcgaacccaa tactagctat atgcgtttag
    26341 cggaaaaaca cggagctaaa ttagtcacat atgacaatgc cacgaatgat cagtatttaa
    26401 gcgatgtagc aaatggtcga acagatgtta ttctaaatga ttattatctg cagaaaatgg
    26461 ccgtatctgc tttaccagat atccctgtaa aaatactgga gaacgtttat ttcaatgaaa
    26521 acgaaagtgg ttttttattg aaagaaggaa atgatgctct gaaaaagaaa atcgatggcg
    26581 tattaaaaga gatgaaagaa gatgggacga caaagaaaat atctgaaaaa tattttcaaa
    26641 ctgatgtttc agtcaaaccg aaagaaacga tggctgagtc ggtgagtgag tgatgtactt
    26701 tcctctaatt gatttttccc tcatgtggac ttcacttcct ttcgtactgc aaggcttggt
    26761 atacacatta gggattggct tcgtgtcgtt tcttttagga aatctggtag ggttgctttt
    26821 gacagtttta ggtcttttgg attggctgcc tctgaatgta tttattcgtt tttatttatc
    26881 gttctttaga ggcattcccg cactagtcct gctgtttctg ttatattttg ggttacctta
    26941 tcaattaagt gccttaacgg cttcagtcat ctgctttacg attaccagca gtgcatttat
    27001 tggtgaaatc tatcgaggat cactagcagg cgtttcttca ggtcagtggg acgctggttt
    27061 agcttctggt ttttctttct ttggggtatt gaaatatatc gttttgccgc aagcttttcg
    27121 aatctcagta ccggcactca gtaacgtagc tatggatcta gtgaaaggta cttcgttagc
    27181 agcaacaatc acagtgccag aaatatttca aaaagcaaag atcataggcg gtcggacatt
    27241 cgattatttt tctctctata ttttagttgc actgatttat tgggtcattt gtatcgggat
    27301 cgaaaaagtg caacagtatt tagaaaaacg ttctatgcat cagtcaatct gaaaaataga
    27361 aatggtgcta gcgtggctgg caccatttct attttttgtt tcttgtatat tctgcctagt
    27421 attttaaaac catttcatgt aaaatgtaaa tcgaaaatat gttcggaggt agaaaacatg
    27481 aagaccccgc taaccatcga aatagaatcc tcgttatacc aatattgcat cgagcaaggc
    27541 ggcttggttg tagaagaagt cacgatgcct gatgaccaag gaattgtaga tacgcttagc
    27601 tgcttgacaa aatttgatgg aagccgagaa tggcgatgct atgagctgaa agtgacaaaa
    27661 gcagattttc gctctacagc caaactgtca tttgttggac attataatta ttttgtttta
    27721 ccaagtaaac tatatgaaga agtaaaaact gaaatcccta caggaatagg tgttttagtg
    27781 tatcgtccgt acacggttga agaggagatg cctgcctctg gtacatttct gattgcgaaa
    27841 aaagcagcaa agaaagagct tgctgtaccg gaagaagctt tgacaaaccg cttcatggct
    27901 tctttattca gggaagtaag aaaagcaaag caaatggagc gaggcgtaaa atattttcca
    27961 acagatcaat tgtacaaaga attgaaaaga cgttcagaaa atcaagatcc attttctaac
    28021 gaacgatatt atgaacggtt tttagaggaa atagctagtc aggcaatcaa tgagttgcaa
    28081 gaagaagtta tcgcgcttca gcatgattat gaatatttga ggcaacaaca gcagatccgt
    28141 cggttgccta cagagccatt ggagtgaaaa atatgtacta tccttattta agaggaaaac
    28201 aatttgacct attagcatta aaagaagcac tgtcgcgagg attgctctcc aataaaatcc
    28261 aaccagtgat cgaaccagta cgtgattccg ctacgcttaa aaatgtgatc gaattatttc
    28321 aaaaaaagca ccatcgaatc gctgtgatcc aaaatcccca agtaggacaa ttcaaattat
    28381 tcgatcagca tgtgcacagt tggacagtca aagaaaatag ctctgtcgtc cctgcacaga
    28441 tcttaacgcc agaaaatctc agtgaagtac tggattcacc tccagcgttt cttgttttcg
    28501 atggacagca ctacccaaaa gatacagagg tgtggaaaca gttggcagga gttgattcga
    28561 aatttttgat accagatact agtcgctttc gcatctggct gcccgaaaac aaaattgtga
    28621 tccgtgactc ttttcaaacg agaaagcatg ttgaaagtta tgcagataaa acagatgact
    28681 tttttagtga tgactatctt tttttccacg cagacggata tattggattt tctgatttca
    28741 cgatcgaagg tagccgttat ttcgataaag ggttccctag ccgagcgctt gctatccatc
    28801 taacgtatat tgatgcttac ggcaatatac gcgtgaaaca ttttgtctct gacagtaacg
    28861 actctgctaa agaccaagca ttgaagtttc ttgaagcagg agataaaatg aaaaaatgga
    28921 tcatgcgtca ccatcatcaa ctcttgatta cttcaggaat gattgaatta ttggctcttt
    28981 accagcaaca aaaattccct ggactgggag tgttgaaaaa gtggtcactt atgcatcatt
    29041 tggaacttat tagtcacctg ctagatcatc caaaagattg gcttaaatat tattcaaaag
    29101 ttcaggaact atatgaagtg atccaatgat tagataatgt aaatgaaaaa ggaatagaaa
    29161 agaggaaact ggatgtatat tcggcaaaca aggaaagaag aagtagaaga agtggtagaa
    29221 atcattaatg ccgcagcgga tttcattgct tcgcaaggct ctcctcaatg gcaaaacggg
    29281 cagaaaccaa caaaagaaac gattttgaac gatattgaaa gaaaagaaag ctatgtatta
    29341 atagtggaag atcaaattgc tggaacagct gcattagtct caagcgtgga tcccgtttac
    29401 acaaaaatag atggtagttg gaaagaatca actggtcctt atttatcgat tcatcgtgtc
    29461 gctttagcag aaagtactcg aggaaaaaaa ctagcaaaac cgttcttaga acatttgttg
    29521 acgattgcag ccagcaaaaa tatccaagat atccgtatcg atacttatcc agctaatgaa
    29581 ccgatggaaa agaccatcta ttctgctgga ttcacttatc aagggatgat tgaatttcct
    29641 tttgaacacg gcgaaagaaa agcatatcaa ataatcatca attgactata aaatcatctg
    29701 agcaaagata aagacattaa cactactgaa aaaaacaatc atatgatagc caagaggttg
    29761 taacagaagt ggccagcttc aaaaaacaag aaagaatttc cgaaaatgct tctcaaattt
    29821 ttgtggaatt ttagcgtatt ttacgaaata gctgctttct gaaactccct tcatcgagag
    29881 gtcgtgacat agcttttgtc acgacctctc ggcttatcct acgagtatta ttcgatacat
    29941 ttatatcaat aatccttttt ttattttatc tgcagcttct tggaacgctc catcgtttga
    30001 cacgactaaa agtgagggga tagctttcaa acgtttcaac tgtgctaaat cttctacata
    30061 aaccgccagt ctggctgaaa tctgttcaga tgaatctcca cgttttttca ggcgcgattg
    30121 caatgtttct ttagagatcg taataaagat aggacggact accgtcccga aatactcata
    30181 taaatgccaa aaaccatttg ctgtcaaaac ggcgtaacaa tttcctactg tcagtttctt
    30241 tttaatttct gctttactgg aaccatattt atgttgagca tattggtccc actctgcaaa
    30301 ctcatttccc ttgatcatct gattgaatgt attgtcagaa acaaaataat aatcctcttg
    30361 atctttttcc ccaggtcttg gtgatcgatt cgtgtaagta atgatctttt gttccggctg
    30421 aaaaatagct tctgcgagtg aagtttttcc agaaccagaa ggaccgataa atacatagca
    30481 ataattcatt gtcatttctc ctttgatgat tgaacaagtg aaaaagcttt ggaaaatatt
    30541 tccaaagctt ttaaaagtaa ctttgctttt ttgtatatta taccagcaag ccagtcacaa
    30601 tagcacttaa aaagctgact aaagtcgctg ttagcaaaat tttcaatccg tgttttgcga
    30661 caagatttcc tttttcttca tttaatccct tcatcgcacc agaaatgatc ccgatagaag
    30721 agaagttggc aaatgaaacg aggaagacag atataattgc tgttgtacgc tcagtaaaat
    30781 ggaaattacc atttgctaat tctgtcatag caacaaattc atttgtaacc agtttagtcg
    30841 ccatgatact accggcatcc actgcttctt tccatgggat tccgctgatg aaggcaagag
    30901 gggcaaagat gtaacctagg atctcttgga aagtgatgcc gaatatcccg tggaagatag
    30961 cattgatcat ggcaatcaaa gcaacaaaac cgataagcat agcagcaacc gtgatcgcca
    31021 catggaaacc atcaagaatg tattctccca acatttggaa aaatgtctgt tctttgtttt
    31081 cttcaatgat cagctcatct tctttttctt ctaatacata gggatttaca attgaagcca
    31141 gaatataacc gccaaataag ttcaaaacaa gggcagtgat cacatattta gaatcgatca
    31201 gtgccatata agatccgacg atcgacatag aaactgtaga catagccgat acactcatgg
    31261 tgaacaaacg tttctcagat aggaagggta attccttttt tatggaaata aaaacttccg
    31321 attgtcctaa aatagcagaa gctactccat tataagattc caatttgccc atcccgttga
    31381 ttttacttaa tcctagacca acagctttca taaataatgg aagaatcttg atatagcgta
    31441 gaataccgat aatagcagaa ataaatacaa ttggcatcaa tacggaaaga aagaaaggtg
    31501 cacttccttt atttgctaca ccggcaaaaa caaagtcaac accttgccca gcaaaagcaa
    31561 gaagatgatc aaacattttt gccaatacag aaacaaccgc tgtaccaaaa gttgtacgta
    31621 ataatacgaa accaagaaca agctgtaaaa cgaacatcac aattagtggt ttcaatttga
    31681 ttgctttttt gttactgctg atcaaaaaag caagccctaa tgtgacaaat aaaccaataa
    31741 tacctaataa ataactcaaa taatcactcc tattccaaat tccgttttat tttacctgtt
    31801 ttgcaaataa aatactaatc tttagtgaaa ttttcattac ttttttagag tgaaacgcat
    31861 tcattttcca ttagaaaata aagcgaacaa aataaaaggt ttaaaaagtg taacgtctta
    31921 gtaaagtcta taaaaaaacg aaactatcga ttaagctaat agtttcgttt tcaacattta
    31981 taagtaaact ttttctattg gtaatataga ttttcggaag ggtagacagg tgcgcaggta
    32041 atatcgattc ctagtttttt caatgtttgt tcatcatttt tattgaccat cacagtagag
    32101 tgggcttgtg ctccctcaag ttcagctagt ttatcataag caacttgagc gattggattc
    32161 gttactgcac tgattgataa agcgatcaag acttcattga tgcttagtgc atccaaagag
    32221 gaatgaagtg tctttgattt caatccttgt attgtttcaa gaactaatgg tgacaataaa
    32281 ggaatgtcat ctgcgatatg ggctaaatgt tttactgcat ttaagataac tgccgctgac
    32341 gcatccatca atgaacttgt acgcccggta atgatgactt catctggtaa ttcgattgca
    32401 atcacagcag aaggatcttt tgtaggactt tgcgcttgaa tcttatctgc atagtcatgg
    32461 gcagggacta ccacgtttcg gtcttcagga cgtaaaccga cttcttccat aatcagtttg
    32521 attcgattaa ctgcttcttc gtcgatcaat ccttttttga agtcgcattc tgtttggaaa
    32581 taacgacgga tgatttcttg tttagaggct tcttggacaa cggtatcatc cacgatccca
    32641 aatcctacac gattcacacc catgtcagta ggagattgat aaacagattc tttaccagta
    32701 attttttcaa taatacgttt gattacagga aatgtctcaa catcccgatt atagttcaca
    32761 gctactttat tgtaggcatc aaagtggaag gaatcgatca tattgacatc ttttagatcg
    32821 actgtggctg cttcataagc aatgttcagc ggatgtttca acggaacatt ccatactggg
    32881 aaagtttcga acttggagta accagctgca tgtccttttt ggctttcatg atacagttgg
    32941 ttcaaggaag tagcgagctt tccacttcct gggccaggag ctgtcactac aacgattggt
    33001 ttagttgttt caatatactc attttttcca aaaccatctt ctgagacgat tttttctacg
    33061 ttgactggat agtcttcgat ttctgagtgt ttatagacct tgatgtttcg tctttctagt
    33121 ttattgataa agactttcgt ggcaggctgt ccactatagc gagtgataac gacactgttg
    33181 attaataagc cataaccacg taattcatcg attaaccgca agatatccat atcataagta
    33241 atcccataat caccacggat cttattacgt tcaatatctc cagcatagac gcaaatcagt
    33301 atttcagctt gatctttcaa tttttgcagc aatttgattt tcgcatcttc ttcaaatcct
    33361 ggaagtacac gtttcgcatg cttgtcgtca actagcttcc caccaaattc taaatataat
    33421 ttgtcatagt ggttcactcg ttccaaaata tacgctgact gctcttcaat atatttttct
    33481 gaatcaaaac caattttttt cactaggttc cctccttaag tactaaagca ttttttcatt
    33541 tttttgagga ttcgtcaact ggaagagcga aattcctcaa aaaaacaaag aaaatggttt
    33601 aaaccaactt gtctagatag ttattcattg gtatgatagt ttttgataag gaaggtgcta
    33661 catgaaaaaa agacagaaaa agaaaaacgc atataaacac tatatccgtt cgatttttac
    33721 cggttatgaa agaatgcttg aagatcccga attggaacaa ttgacgttta cttatctaaa
    33781 cgaagaaacg cagctaactc gtgatgagca tcagcgcatt catttcacaa caagagatct
    33841 gcctagtaaa taaaaaataa gataaaggtc atcggattcg attctgtcct atcagatgga
    33901 aagactagtt gatcgaaaaa aaggctgtac caaattttgg tgcagcctgt tttatggagc
    33961 attgcccgct cttttttata acaatttttt acagaaaatg gtactattga agtattaata
    34021 agtgaaaaac agatgagtaa gaggaggcaa gtagtatgaa agagccagat acaaatgcaa
    34081 attatgagcc agacgaacta gaggagacga atgcggatca ggatacagga caagaatcgt
    34141 cttcttttta cgatatcaaa gaaaaagcaa gcatgaatct ttcccaatta ttagaagact
    34201 tacaggcttt tgaaaaagct gtccgcgaag aagatatccc tgagatctac cgtttataca
    34261 atggccgatt gaatgaagaa ttaaaaagaa gctcaaatga aaatcacgag atcgatcaat
    34321 tgctgatgcg taagatccat gatcgttttt tacaggaatt tccttttatg gaacaagtag
    34381 aaaacatctc gccaacgatg agctattaca aaatcggcac ttattaccat gatcggccca
    34441 cggtcggtat tgatgcaagt ctgccagaaa tttttgtttt gcctaagatc gatgaagagt
    34501 gggagaaata ttcccaaccg gagtcaacgg actatgacaa aaaaatcaat gagctagacg
    34561 caaaggtgat cactgctcag acagaaattg aacgtttaga tgaacaaata aaagaaatca
    34621 atcgacaaat gactgatttg aacgacaata aaggattctt gaatcgtaaa aaaatcgagg
    34681 aagaaattca agaattagag aagaagaagc aagtactatc taatgaaaaa ttaggctggc
    34741 ttccttatat tgagacgcct gaaacaatcc agcagcaaaa agaagcattg aagcaagaag
    34801 caagagcaga ccagcttcgt gcagccatcg tagaaaaaga acaaaggcaa atcagacgct
    34861 atttcggtag caaagaggga ttcggtcaag caatccatga attcttgatg aattatcttg
    34921 gcaatgagaa tcctaataat caatctaatg aaggaggttc cgagtatgaa tgaacacgat
    34981 gaagcagaat ggacaaaaaa tgaagaacaa acagaagatc atgaagaaac gaacacttca
    35041 ttggaagaac aaacaagtaa tcctctggat gacacagaag aaatccctat gctagaggaa
    35101 acaaaaatac acgaaaaaga aaaagtaact ccgacgaata cgaatactgc agaaaacacc
    35161 catgcttacg aaataactga aaatcccggg tatttagaag gaaaagagca agatcatcaa
    35221 gatatcgtca gtttgattga aacagcggaa gcagaattag cggatttacg tatgagaaaa
    35281 gatttgatcg aatcagattt tccacaatta ttagaatcat atgatcgttt tatattgaat
    35341 gaagaaccaa tctccacgat cggaaagaca agcctgatcg aatacttcac gtatgaatta
    35401 ctgaaaccgt tgaatgatat tggtctggaa cggtcaaatt cagcttatga cacatggaaa
    35461 acaagacatt tttcgatcaa ttatacgttg aatggacaaa acgaattagt cttttccttt
    35521 gtattaccga ccatcgatca acgataccaa gtggaatcga tgagtctcct aaacgtatca
    35581 ccagagacaa tggaaatcaa tgtccaagat gaccgggtgc tttctttgat ccgacactgg
    35641 tcagtggatc gagtattctc tgcagggcag atcacgatat tcaatcacaa attgaatcaa
    35701 ttattagccc atgcaagaac acttggtttt accgttaatc agacattact agacaatacg
    35761 aagccacttc gtttgaatct tcagagcgaa tttgaattga cgaatgaagt attggatgat
    35821 atttttatcg tggcaatgaa aaatccatcc tacgatatgg aaaagatcaa cgaaactact
    35881 tatcaagtac gattagataa aggacagtct ttaacgataa caacaaacga aaaagatcaa
    35941 acagtcttgt cgatctcatc tggagactac ccacgatctg tcattgattt ctttatcaat
    36001 tacgaattct tggtcccatt aatggtgaga aagtcctgat tggataagtt ggattaccag
    36061 atgtcattca gataattctg ttccaaagga cttgcggcag ttatccagaa taattgtcac
    36121 aagtcttttc tgtatgtact aaatggacgt gaataacttg ctgatgaagg agggatttca
    36181 ttaggaatgt ccacaaataa aatagcacag cttagcttat tgtctgctgc ttgtgtagca
    36241 ggtaggatcg cttttcaatt tattccgaat ttccagccaa tgactgctat ttttttattt
    36301 atagtacttt acttaagctt aaaagatgcg ttaattgtca tgagtctttc tattgcgatc
    36361 agcagttttt attttggtgt tggaccatgg gtcatcggcc aatggatcag ctatactgtt
    36421 gttctgagtt tgttttcaat cgtctgcttg agaaaaacag tgcaaaggaa tctatggtta
    36481 aagggagtct gtttttttct ggcgggcatg gtttacggaa taggtatgac cgtttttgat
    36541 acgctgttat atcgtttgcc tcaaccttgg atctattacc ttcaaggggt gtcgtttgat
    36601 ttgatgcaca gtataggcaa tgttgtgttc tttcttgttt ttctgccagt cgtcaaacgt
    36661 ttttacaatc attcaggagg aaaaaatgaa aaaaataatt tatagtttgc ttgcaatgag
    36721 tgtcagtata ctcattttga caagttgcag tacaacctat acacaatcgg cttccaacca
    36781 aaaggaggaa gtaaaaacat cgcaaaaagc aacaatcacg ttacaagaaa atggtcaaaa
    36841 cttttcggaa aaagaagtgt tcttcaaaaa aggcgatgat ttactgagta ttttgaaacg
    36901 aaattttgaa gtcaaagaag ataacggctt catcacttcg cttgaagggc actcacagga
    36961 tgagaaaaat caactctatt ggatgtttac tgtggacggg aaaagtgcta ctactggtgc
    37021 caaagagatc aaattgaatg atggacaatc ggtcattttc aatttgtcta aactataagt
    37081 ttcataaaaa actgttaaag ggcgaatatt gccataattc agtgagttca gttgatgaaa
    37141 gaaggaaaat aacatggaaa attttaaaaa aagcggaatg ttttccgtaa ttgctgctct
    37201 aggggctaat attctagttg ctatcagcaa atttgtcggc tatgcaatca gtggcagtgc
    37261 cgccatgcta aacgaaagca tccacagtat cgttgattgc agcaatcaga ttttgctgtt
    37321 attcggtgat aaacaagcca ataaaggaca gagcgagttg catcaatttg gtgaaggacg
    37381 agcaaaatac tttttcagta caatcgtcgc aatgatgctt ttcttcggcg gtggggcact
    37441 tggggtgatg gaagcttttg aaaagttgac gcatccatcc catgaagtgg gtaatccgat
    37501 cattgttata gcaatactgt tatttggttt ggtaatcgaa ggaagttcgt tacgcgtggc
    37561 aatgaaagaa atcaaagaat tgaatactga aaaactttcc acgatgcgct ttttgcgcga
    37621 aagtcgacac agtgaaatac tgattatttt tacggaagac ttgtgtgctg taatcggttt
    37681 ggtcttggct ttagcaggaa caatattgac ttatgcaacc gggattgctg catttgatgc
    37741 gatcagcgga ttgctgatcg gtttcttgct gatgggagcc gccattttcc tagcaaaaga
    37801 attttatagt ctgatcatcg gtgaaagcgt gacagcaagc gatttatcaa aaatcaagtt
    37861 agcttttgaa cgtcctgaga tcagtcggct gatcgatata aaaacagttc atttaagtcc
    37921 aacggagatt ttagttgcag caaaagcaga tatcgttggt aacaaagaga atgaagctta
    37981 ctcggtcatc aatgatattg aaaaaaatat gcgtcagtct cttcctgaca agaagatgta
    38041 tatatacatt gaaacagata aatatgaccc aaattattct cgtaaataaa acgtttattc
    38101 atgaattgaa gcaaaaatga gaaaaatata atcttgtttt taaataaaac ggctacaatt
    38161 tagcgaattt atgttacaat gagtccgctg tttatttgtt gtaattaaag gagatatttt
    38221 aatgaacaaa aagaaaagtg cgccatggct tcaagctttg catgcagtga tgccactttg
    38281 tataagctat ataccagtag gattagcttg cggagtattg ttgcaaaaag ttggatttga
    38341 tcctctctta tcaggactgt tatcgattct ggtcttttcc ggtggcgcgc agtttttagt
    38401 tgcatctatg cttacgacgc aagcatcatt tgcaacgaca ttattgatgg ttttcttttt
    38461 agaattacgc tatacattat taggagcaag tcttgccggt tttatgaaaa aagaaaagcg
    38521 gacgtttttg gctgtctttt ctcaatcact gaatgatgaa aactatgcgg tgaactactt
    38581 aaagttttca actgaccctt cctggaataa aagaaaggct ttatatgtca attggttctc
    38641 aatgactgca tgggccggta gcaatatgat cggtaatttt ttcggctcgg tgattcgagt
    38701 agatgctgac cttgtccatt ttgctttgac ggccatgttc attttcatgt ttatcatgca
    38761 gatgaaaagc gcactactga tctttacagg tttattctca ggcgtattag gggtcgtatt
    38821 catggtatta ttccaaaata ctttcggatt gattgcagca acagtcattg cttcatttgc
    38881 cggatttatg ctggagaagg cattcaaaaa agaaaaagct aagaaactaa gagaccgcgg
    38941 taaagatgtc aatgagccgc tcttcaactt cccggaacag gaggccaatc accatgacta
    39001 aactatattt gctactttta gttgcttgtc tgttcatcgt ggcatatatc ccgcgcttgt
    39061 tcccgatgct ttattttact catcgaaaag tgccatcctg gttcagtgac tggatgaaat
    39121 atgtaccggt ggcattattt gcggcgttag cttttaaaga tgttttcatt actcatgaac
    39181 atctggatat tgcttggaat atcaaaatag ctgcaatgat tctagtagca ggcgtggctt
    39241 acaaaacacg ttcgatggcg ttgtcagttt tgacaggact tgcgtctgtc tttttgctgt
    39301 ctatgcttta aactgtttta ttttattaaa aacagagatt gcgacataag ttgttcaaac
    39361 tgatctatcc gaactgcatt tgcaacagtc aattctggat gcaagtttat tcgtagggat
    39421 aaaaaagatt agtgttggct tatgatgcaa gctctttttt attttaggaa ttagagaggg
    39481 atttgtcctt ctatgatttt ttgctataat agggaagatg tttattttat gaaagaatag
    39541 ttgagggaat ttaaatgaat aaaaaattga tgcaaggaac attttggcta acctttgcta
    39601 atcttctatg taaagtttta ggggtagtct atttgattcc ctggctaagt atgatgggga
    39661 acaatcaaga tggtatgctt gctacgacat tgtataatgt tggctatctt ccttatggat
    39721 tgttcctgat gcttggtaca gtcggatttc caaatgcgat tgcaaaaaaa gtagctgtag
    39781 ccacaaaaga aggagataac gctgcttgtc ggctgatttt tagaagtacg atcaatatca
    39841 tgtttgtcat cggtatcgcc tctgctgcac tgatgtattt atttgctcct ttactagccg
    39901 gaatcagtcc gattgctaac gttaataatg ggatactagc tatcagaagt ttatgcccat
    39961 ctttgattgc tatcccaatt cttagtgcaa tgagaggata tttccaagga aaaaatgatc
    40021 tgcggcctta tggaacttct ttgattatcg aacaggctgt gcgagtgatc gttatcttgg
    40081 ctggtactta ttacttgcgc gtattgactg acggaaccat attagaagct gtattgatca
    40141 gtacagtcgc ttctttcttc ggtgggttag ctgcgattat ccatatgtat atcgtcggac
    40201 gcagaaaaga cttcttcgaa cttaaagatt ttttgatttc cagccgttat ttcgaaagag
    40261 aaaaccgatc tgcctctgtc tctatcattc gggaaacact accgtttatt tttgtcggct
    40321 cagtcattac tatcgttcaa atgatcgacc aagtaacgat gaaaccactg cttcactttt
    40381 tccgtccaga gatcgcagat cagcaactgg aattcttatt cagccgtgct tctgtcaatc
    40441 caaataaatt gacattgatc ctgatctcca tggtaggaac agtagctata agcagcttgc
    40501 caatattaag cacgatgaag aaaaaagatc ggatacaaat cgaaaaaaca gtaggagata
    40561 gtttttcgat tgcgttattg attcttcttc cttcattgac tggaatgtct ttattagctg
    40621 gaccactata cacattgttt tttggctatg atcctgaaag tgtcggatat ttccaaatgg
    40681 ctttgctagc ttctttgttt ttctctttat tcacgatcct atctacgatg cttcaatctt
    40741 tgaatcacca tcgttttgcg atccgattaa ctgtagaagc aattatcctg aaagtcatct
    40801 tccaagtaat cggattagga ttagttggcg gatacgggat gagcttatca aatggcttag
    40861 cattcggtat tgtctttgtt cgcggctatc actttatgtg caaagaatac cgaatagcac
    40921 ctttagcaaa gatcagcaat tttttcttaa aaactttccg ctccaccctg attatgcttg
    40981 cagtatgttt tgccgttttc tttttgctga atcagcgatt gtcaatggaa tcaaaaagct
    41041 atgcaatgat ttattgtgtg gctgtaggaa gtattggcgg cttagtattt ttattttctc
    41101 aattcggaaa aaatgggttt cgaatgttga aaaatgttaa acatcatagc agaagcaaag
    41161 aataggttaa ctaatcgttt tttgctgtca cttgaaaaac ggcaactcgt atgattacgc
    41221 ttgtcgtttt tctattttcc aattttagtt tcatatctaa tcttacttat gatatagtta
    41281 taaggtctgg aaaaattctt tgaaacgagg aaattaaaca catgaaactt gcagtagtta
    41341 cagatagttc ggcatatctg ccggaacgta tcaagaacca tcccgactta ttcgttattc
    41401 caatcccttt gattatggat gggaaaattt ataacgaagg aatcgatatc gaagcggatg
    41461 aatattatgg acttttaaaa aacagtaaag attttcctac tacttcacaa cctgtcgtag
    41521 gggaagtttt gggtttatat gaggaattga aagagaaagg gtatgacacg attttaagta
    41581 tccatctttc ttcagggatt tctggctttg tcaacacatt atttgcgatg aaagatgaca
    41641 tcagcggagt aacggtcatt ccttatgact ctaagatcac tagtatgccg atgggacaca
    41701 tggtagaagc tgccttagac ttaaatgatg aaggtcagtc ccttgatgaa atacttgcac
    41761 atatcgatcg aatccgagat aatacctacg cttatctgat tgtagatgat ttgaacaatt
    41821 tagtacgagg aggacgtttg acaaatggtg cagcgctgat tggcagctta ttaaaaatca
    41881 aaccgatcct gacattttct gaagggaaaa ttgttttatt cgaaaaaatc cgttcaagta
    41941 aaaaagcatt tgctcgagct gaagaaatta ttggtagaag aaataacgaa atacaaccgc
    42001 cagtcaaact ttacgtgata catgcaaata atttagaagt agcgattgcg gagaaagaga
    42061 aattgcagaa aaaatatcca aatgcagaaa tcgaaattgg tcatttcgga ccagtcattg
    42121 gtacacattt aggtgaaaaa gccatcggca ttgccatttc agcgcaataa ttccttttat
    42181 tcaaaaaaca taccaaaaat attccaacat catcttggac tttctacatg ttactaatga
    42241 gcgtgtaaaa gaacgagatg gtgtttttat ttctatcaat gaagctcttt ttctttgcaa
    42301 tcctcacttc aatcctctat aatgtcgcat atagtttagt aaatgagtaa actactaatt
    42361 tacttattta ctaaaaataa aaggatgagg agttaaaata atggaaatac caacaaattt
    42421 aaaacataaa ccagtaatcg ttgtagaaga ctatgataaa gttgatggaa gaaatgcatt
    42481 aaatacggat gcaaaagggc tttcactggg ccttgctcag tggaatgatc gtggaaaagt
    42541 cgatatctca ggcaaggtat ggcgacatac tggagaaaaa tggtcacgac agtcggaaga
    42601 attaccaatc acacgtattt tagatttagc gatcttagct gcacaaggaa gtttatactt
    42661 ccaagatgct tatcgttatg aaaaatttta tgatccagaa aatccagtcg tagacatcat
    42721 tggattacaa gggaaccgaa tgacggtaga agtagatacc aaaaatccaa agatcgatga
    42781 agacatcatt ttgtattacg atacactaca aaaagacgga gaactgattg gggaacgatt
    42841 ccgtatttta aaacgtcttt tagatgagct aggatattaa tagacagaga tgatcaaaag
    42901 gggaagagaa gcatggagaa tttctcacta acagaaaacc gaaccttttc acggctagaa
    42961 aagcaaatga tctggaagaa accgaaaagc catcggacaa gtgcagaaga atggcggatc
    43021 gcctccgaaa tcaaagggaa ttggcaagat cctgaaatga aaatcagtaa cgtacttttg
    43081 gaaggagatg ccgggtcggg gaagacacaa ttagccaaag cactatctgc tgatctgcaa
    43141 cttccctata caaaagtgac ctgttttgca gatatggaca aatcagatgt tttcggagcc
    43201 ttattacctg tagtagattc taataaggaa caagaccaag aattactaga agctatttac
    43261 caaacagata cacttgaagc tgtactgaca ctgatccaga accattacgg cattgatcct
    43321 ttttctgcta aagaaaaatt agcagagcta gttcaaagga tcgaagctga ttatccagat
    43381 gcaatccatt acagctatta cccttctgaa atcgttcgag cgattgaaaa aggatattta
    43441 ttagagattc aagagccgac tgtcatccga gatgcctctg ttcttgtggc acttaactca
    43501 gcgctcgaac caaatgggat gttgaattta ccgacaggaa tcattcgtcg ccatccagat
    43561 tgcgtcattg tgatcactac aaatcgaaat taccaaggaa atcgcccatt aaatgaatct
    43621 ttaagagatc ggatgcaaca tgcggaaaaa atggatcttc ctgccttaga agtcatggca
    43681 gaacgtgcga tggcgaaaac tatgatccaa aaacaagaac ttctcttaaa gatggcagag
    43741 attatccgtt tgttggatga aactgcaaaa gctaacgcca tcaaaggtgt agcaggaatg
    43801 cgttcttatt tttattgggc aaatacctat aagcaaggac aagatttact ccagtcgatt
    43861 tatccaaaag ttttgtataa attatccact gattcagatg aattggcaat tcttgaacaa
    43921 gctttgtctg acagtggatt gctactggaa cttcaggaca tattgagaaa agaaaaatgg
    43981 ggtcatcctc aatctgagca tacaaaagga agaaccatta gcgccgaaga agcaaatgaa
    44041 cgaaacatca ctgaaaatgc tgaagaaatt ctgcgcttag atgaaaagaa atcgttgtct
    44101 gctgaagaac caaatgaggg agaagagaaa caagaagaaa caatagaaaa aaacgagatt
    44161 cctcctcaaa tgcaaaaaga acgcagtagc gatatcgaag gaaaaaatca aaaagaacaa
    44221 caaacaggag atgattctca agaaatgtct gcttctgaca tggaagcaca tgataaggaa
    44281 atcaagaagc aactgaataa agaagcgcga caaatcatga aggaaacgat tcatgaaaaa
    44341 gaaggtctca tcgttcaccg accagctttc caaggaaaaa cgccggaagc acgtgagcta
    44401 cacatgcaag taatgccaat agtagaatca cttagccggc aaattttaga gttactggat
    44461 aatgaacaaa cagagacgta ccaaaaagga aaatacgaag gtcagcggtt caatgcaagc
    44521 aaagtagcat atggtgattt gagaaatttt gacaaaaaga acccgccaca tgagcaacct
    44581 tctttagcgg tagctgtacg gatcgatgaa tctgggtcaa tgatacggga tgatcggatc
    44641 gaagcggcaa aaaaagcagc gattgccatt gccgaatttt ctaagaaagt cgatatccca
    44701 ttgttgattt acggagatac agcagaccgt tcgtcaagag aaaagacttc tttgttttct
    44761 tataaagaat ttgaagacgg atttcaatgg ttaaatgaaa aattagtcac tatgaaacca
    44821 aggcaaaaca atcgtgatgg tgctgcactt cgtttagcag cagaaaaatt aaacagacaa
    44881 tccgcaacaa ccaaactgtt gcttaatatt agtgacggtc agccaaaagc gttgccggat
    44941 tatacaggca aaaaagcaaa agaagatatc cagtctgttt tgaaagaata tgaacgtcaa
    45001 ggaatcctat ttgtctccgc agctattggc caagacaaag aagaaatcaa gtcgatctac
    45061 ggtgaaagtc gttttgtaga tattactgac ttgaatgaat ttcccaagca aatgattcaa
    45121 ctgattgccc gctatctatg ataaaataaa gctcaacgaa cgcacttagc agattcgttg
    45181 agcttttcgt ttctagttac cggtaacaag gatacttcta aaatagaatc ggttataatc
    45241 aagagggacc gttaaaggag tgaaacgata tgatacaaga acaaaaatgg tggaaagaag
    45301 cagtaggcta tcaaatctat cctgctagtt tcaaagatag caatggcgat ggtcaaggag
    45361 acatcaatgg gatccgtgaa aaacttaatt atttgaaaga tcttggtatc ggctttatct
    45421 ggatcaatcc gatctatcaa tctccatttg tcgataatgg ttatgacgta agtgactatc
    45481 aagatataga agagagattc ggaacgatgg aagattttga tttattgcta aaagaagcac
    45541 atgaattagg gatcaaagtg attatggatc ttgtcatcaa ccatagttcg gatcagcatc
    45601 aatggtttga agaatcgaaa aaatctaaag aaagcccgta tcgcgattat tacatttggg
    45661 tcgacggggt agatggcaaa gaaccgaata attggacatc gattttcggc ggttctgctt
    45721 gggagtattc tcatgaaact ggacagtatt atctgcatgt ttttgcaaaa gaacaaccag
    45781 acttaaattg ggaaagcgag aaattgaaag aagaactatt caatatgatc cgatggtggt
    45841 tggataaagg gatagatgga tttcgtttag atgcgatttc tcatgtaaaa aaagatgaat
    45901 attcagtaaa agccacagag aaccctttct caccctttca aaatgtctca ggaatcgaag
    45961 aacatttgac cgatttgaaa catgtttttg aagagtacga tatcatgact gtcggagaag
    46021 caagtggtgt cactgcagaa gaaggaccac aatgggtagg gaaagacggt tatttcgata
    46081 tgatattcga atttgatcat atccatatct ggcagcaaga aaaagaaggg caattagatg
    46141 tccttaaatt gaaacttgca ttaagcgctt ggcaaacttc attggatggt atcggatgga
    46201 acgctttata tatggaaaac catgatgttc ctcgtgccgt ttcagtgttt ggagatactc
    46261 gtcccgattt ttgggctatg tctgcaaaag caatcgctat gatgtatttc tttttgcaag
    46321 gtacgccatt tatttatcaa ggacaggaaa tcggaatgac gaatatgccc tttgaatcga
    46381 tcgatcaagt tgatgcagta gattcgaaaa gactttataa aaggctgctt gcggaaggta
    46441 agacaagaga agaagcattg gatatcattc gtgaaacaac gagagacaac agcaggacac
    46501 ccatgcaatg gacttcggaa caatatgctg gtttttctac acatgaacct tggctgatca
    46561 ctaatccaaa taccaaaacg atcaatgtag aacaacaaga atatgaacca gagtcggtcc
    46621 tgcaattcta taagaacatg atccgcatta gacaaaccaa taaaggtctg atatacggtt
    46681 cttataaaga atatcttcat gaacatccac aactttatgt ctatgaaaga tatttagaag
    46741 atgaacaata tcttattatg gtaaacttaa cagaatcact tgctgactat gaacttccaa
    46801 aagaagcaga tcagtcctgg acattactct tgtcaaattc ctctagcgga gaatttgaag
    46861 ctaaaggcat actagcacct tatgaagctc ggttatataa aacaaacaag taaaacttcc
    46921 tcgttaaagt tcattagttg gactttgcga ggaagttttt cagattgctc tgtcaattac
    46981 tcatttcaat aattcgctct aggaccaccg atatatttag gacgagagcg aagggctgtc
    47041 acgtgagctt gtccaagggt tttcaaacga ggtctgacag gtacttggac atgtttgata
    47101 tgcataccga ttgatgtgtc accgatatcg ataccggctt gtccaactac gtgttccact
    47161 tcgacaggat cattgaactg gtcaaaagca gcaacagaac aagcacctcc agcatgtagt
    47221 gcaggaacaa cagagacaat ttcgaaattt tttgcttctg ccactgttcg ttctactact
    47281 aatgctcggt tcaaatgttc acaaccttgg acagctagcg caatttcttt aggatccaac
    47341 aactctttta acgtacggat gatccattga ccaacttccg cactggaatt ttttccaata
    47401 tgtcctccaa cgacttcgct tgtgctgcat cctaaaacaa aaagatcacc ttttttcaag
    47461 tgtgcctcag ccaatatgtc attaactatt tcagtcaatt ggtcttttaa ttctttttct
    47521 gacaacttca ttttctaaaa ccttcttttt caattaatat tgcttccgtg cttgaatacg
    47581 ttgaagactt gcagttatag gaaaggcaag tcccattcca atcagatttt ggaagatgtt
    47641 tcctggtata gaagccactc cagccggcca atcatataaa atccaaccgg cgataccata
    47701 gccgatgatc ataactaagc ttcctaataa aagccctaat ccccacatac gagtagaaga
    47761 tttgctgaaa gaaccagcaa catatccttg taacccgtgg atcactaatg aaaaaactgc
    47821 ccactgcgga tatcctgaaa tcaaatcgat caatagacca ctggaagcac caacaactaa
    47881 tccacccgtt ggaccataaa gaaaagcagc ggtatagatc ccggcctcgc ataatgtcac
    47941 aaatccattc gtagccggta cagggatcaa aattgttaat gaaagggcaa ctgtcaacgc
    48001 aatcagcata gcatagatcg ttaattgctt agttttcatt tctcattcct cctttttgaa
    48061 taccgccaga ccagacactt ccggtatgat cgttcatcag cacgccatta gaaatacaat
    48121 gggcaacaaa ttttttactt tctgctactg ctttggtaaa atctgcccct aacacaagat
    48181 tcgctgttat tgcagaagaa aaggcacaac cggccccgtt tactgttgta gtgtcgattt
    48241 ttttatgaga aaatacctca taatgatcaa ttgaatagag aagatccaca gcttcatctc
    48301 cagccatcct tgtgccgcct ttgatcacaa tgttttttgt tcctgaagca gtgagttctt
    48361 tagcgagatg gatcatttct gttaacgaag agggaagagc ttgaccagaa agtaaagcag
    48421 cttctgccaa attaggcgta atcatcgtaa caagtggaaa aagttcttcg atgagctgcg
    48481 aaatatacaa ttgattgatc acagcttcag tttctttgaa tgccaaaaca gggtctaaaa
    48541 tgattgggat attttgtttt gtttttatga agtctcgaac aaccatgatt gcttcacttg
    48601 aattcaataa cccaattttg ataccatcta atgaagtcat tttatcaatc gaaactaact
    48661 gtcgtttaag caattccaaa gatatagatt ccagcataaa tcgctcatta atatctagtg
    48721 cccctataga agtcaaggcg cttagtccga atagaccaaa attctcaaaa gtttttaaat
    48781 ctgtttggat tcctccacca gcaaatggat cagaaccgcc aattgtcaag attcttttca
    48841 taacattttc ctccatgaat aagagtataa cgaaattttc aaaaaagaaa aacagccaat
    48901 tggcatattt ttatctcatc caatttaaaa aaacaaaaag aaaagaaaga ccaaaatgga
    48961 tctttctttc cttaaatagt taggactttt gtgattttaa ttctttctcg atttgaagcc
    49021 gttgcacttt cattgtagct gttcgcggga ttgcatcaaa atcacggatg atagggacgt
    49081 tcaagcgagg aagttcatgg acttgctccc accatcgatt ccaatccatt tctttatcag
    49141 gagctaaagc aataatcggt tgtggagaat gatttttatc ccttacaatc actacttctg
    49201 ccaaaaaatc taacgaatct aataaaaagt cttcaatagc taatgtacta ttgatcgttt
    49261 cgatcaaatc cacttgccga tctttcaaaa ataggtggcc atgcttatcc ataaaaccgt
    49321 aatctccgct atcccaccaa tcgccataaa cattttcctg gaaccgtgca tcttccttgt
    49381 aataagtcaa agcccgtcct ttagacagta attgtatatg accatctgtg ttttcaggta
    49441 ggacattccc aactgaatct gtaatccgag cgcttgtcat gtcttccagt ccaacgccca
    49501 tatctcttgc atcacttgtt ttcaaactct ctagtgtatg agccttaaga atcattggtc
    49561 cgcattcact ttggccatat acttgaagaa ataccgcatc ctgtgccatc gacgttcgca
    49621 aaaatgtctt cattgtctgg ttgtttatag catcaaaagt ggagtgatag tatcggatac
    49681 ccgaaaaagc ttctggatgc tccttagcag taaaacgcca ctgaacaaag ttattcggat
    49741 gggtctctag tgcaattggc cggtgagctt ccaacatatg aacaactttt gaactttgtg
    49801 cgtttgctaa aggcatcata ggaaatccca tggacatcaa agaagagata ccaatattga
    49861 atctggaatg cactggagag atgtgaaaag cgattaattt cttttccgaa atttttgtga
    49921 aaactgtttt ttgccatttc gtccgccatc ccatagaatg agcagaatga caaataagtt
    49981 tagggatacc agttgtacca gaagtatggg tcatataaga aatttcattt ttgtctaaaa
    50041 atgattgaga aacagacaaa gcaggttgtt ccattaaata aagaatagga a
//
LOCUS       DAZABD010000013        49314 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000013 DAZABD010000000
VERSION     DAZABD010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49314
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.013"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            349..828
                     /locus_tag="ACHALG_000828"
                     /pseudo
     CDS             349..828
                     /locus_tag="ACHALG_000828"
                     /inference="COORDINATES: protein motif:HMM:NF016901.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            862..1818
                     /locus_tag="ACHALG_000829"
     CDS             862..1818
                     /locus_tag="ACHALG_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2586257.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
     gene            complement(1911..2645)
                     /locus_tag="ACHALG_000830"
     CDS             complement(1911..2645)
                     /locus_tag="ACHALG_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFN2586258.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            2971..3381
                     /locus_tag="ACHALG_000831"
     CDS             2971..3381
                     /locus_tag="ACHALG_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFN2586259.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAGRVSVNEMEENQID"
     gene            3374..4075
                     /locus_tag="ACHALG_000832"
     CDS             3374..4075
                     /locus_tag="ACHALG_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFN2586260.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            4075..5688
                     /locus_tag="ACHALG_000833"
     CDS             4075..5688
                     /locus_tag="ACHALG_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN2586261.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKKTIKTITKKEKKDDI"
     gene            5678..5899
                     /locus_tag="ACHALG_000834"
     CDS             5678..5899
                     /locus_tag="ACHALG_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HFN2586262.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            5896..6657
                     /locus_tag="ACHALG_000835"
     CDS             5896..6657
                     /locus_tag="ACHALG_000835"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HFN2586263.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEELSLRFPETFAYALGDVRQMKELQQAVSKCQDRFGEVTILLNTSGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRRKSGTIINMASIAGLVAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            7038..7547
                     /locus_tag="ACHALG_000836"
     CDS             7038..7547
                     /locus_tag="ACHALG_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFN2586264.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANMASPLGLIDVVVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(7617..8156)
                     /locus_tag="ACHALG_000837"
     CDS             complement(7617..8156)
                     /locus_tag="ACHALG_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HFN2586265.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVAVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(8296..8907)
                     /gene="rpsD"
                     /locus_tag="ACHALG_000838"
     CDS             complement(8296..8907)
                     /gene="rpsD"
                     /locus_tag="ACHALG_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFN2586266.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            9169..10020
                     /locus_tag="ACHALG_000839"
     CDS             9169..10020
                     /locus_tag="ACHALG_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFN2586267.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPAATILVEGMFANIVVNTAVLISMRMKEDAGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLAYFASQGQTDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            10048..10683
                     /locus_tag="ACHALG_000840"
     CDS             10048..10683
                     /locus_tag="ACHALG_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HFN2586268.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVYYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            10703..11476
                     /locus_tag="ACHALG_000841"
     CDS             10703..11476
                     /locus_tag="ACHALG_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFN2586269.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEKQEVYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            11884..12681
                     /locus_tag="ACHALG_000842"
     CDS             11884..12681
                     /locus_tag="ACHALG_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HFN2586270.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            12659..13072
                     /locus_tag="ACHALG_000843"
     CDS             12659..13072
                     /locus_tag="ACHALG_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFN2586271.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEQEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            13056..15701
                     /gene="mutS"
                     /locus_tag="ACHALG_000844"
     CDS             13056..15701
                     /gene="mutS"
                     /locus_tag="ACHALG_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFN2586272.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            15719..17827
                     /gene="mutL"
                     /locus_tag="ACHALG_000845"
     CDS             15719..17827
                     /gene="mutL"
                     /locus_tag="ACHALG_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFN2586273.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEEPKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     LQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            17849..18409
                     /locus_tag="ACHALG_000846"
     CDS             17849..18409
                     /locus_tag="ACHALG_000846"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFN2586274.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(18494..20482)
                     /gene="treP"
                     /locus_tag="ACHALG_000847"
     CDS             complement(18494..20482)
                     /gene="treP"
                     /locus_tag="ACHALG_000847"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HFN2586275.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            20705..21418
                     /gene="treR"
                     /locus_tag="ACHALG_000848"
     CDS             20705..21418
                     /gene="treR"
                     /locus_tag="ACHALG_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HFN2586276.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            21495..21941
                     /gene="msrB"
                     /locus_tag="ACHALG_000849"
     CDS             21495..21941
                     /gene="msrB"
                     /locus_tag="ACHALG_000849"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFN2586277.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            22111..22713
                     /gene="ruvA"
                     /locus_tag="ACHALG_000850"
     CDS             22111..22713
                     /gene="ruvA"
                     /locus_tag="ACHALG_000850"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFN2586278.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            22726..23727
                     /gene="ruvB"
                     /locus_tag="ACHALG_000851"
     CDS             22726..23727
                     /gene="ruvB"
                     /locus_tag="ACHALG_000851"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFN2586279.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            23756..24334
                     /locus_tag="ACHALG_000852"
     CDS             23756..24334
                     /locus_tag="ACHALG_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN2586280.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            24386..24868
                     /locus_tag="ACHALG_000853"
     CDS             24386..24868
                     /locus_tag="ACHALG_000853"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFN2586281.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            24985..25359
                     /locus_tag="ACHALG_000854"
     CDS             24985..25359
                     /locus_tag="ACHALG_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2586282.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            complement(26159..27511)
                     /gene="brnQ"
                     /locus_tag="ACHALG_000855"
     CDS             complement(26159..27511)
                     /gene="brnQ"
                     /locus_tag="ACHALG_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFN2586283.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
     gene            27658..28248
                     /locus_tag="ACHALG_000856"
     CDS             27658..28248
                     /locus_tag="ACHALG_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFN2586284.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            complement(28376..29725)
                     /locus_tag="ACHALG_000857"
     CDS             complement(28376..29725)
                     /locus_tag="ACHALG_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFN2586285.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(29893..30633)
                     /locus_tag="ACHALG_000858"
     CDS             complement(29893..30633)
                     /locus_tag="ACHALG_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFN2586286.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            complement(30646..32238)
                     /locus_tag="ACHALG_000859"
     CDS             complement(30646..32238)
                     /locus_tag="ACHALG_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFN2586287.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     regulatory      complement(32376..32550)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            complement(32806..33102)
                     /locus_tag="ACHALG_000860"
     CDS             complement(32806..33102)
                     /locus_tag="ACHALG_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2586288.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            33278..34003
                     /locus_tag="ACHALG_000861"
     CDS             33278..34003
                     /locus_tag="ACHALG_000861"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HFN2586289.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            33996..34838
                     /locus_tag="ACHALG_000862"
     CDS             33996..34838
                     /locus_tag="ACHALG_000862"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFN2586290.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            34841..35362
                     /locus_tag="ACHALG_000863"
     CDS             34841..35362
                     /locus_tag="ACHALG_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HFN2586291.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(35615..36178)
                     /gene="def"
                     /locus_tag="ACHALG_000864"
     CDS             complement(35615..36178)
                     /gene="def"
                     /locus_tag="ACHALG_000864"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFN2586292.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            36429..36698
                     /gene="rpsO"
                     /locus_tag="ACHALG_000865"
     CDS             36429..36698
                     /gene="rpsO"
                     /locus_tag="ACHALG_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFN2586293.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            36886..39012
                     /gene="pnp"
                     /locus_tag="ACHALG_000866"
     CDS             36886..39012
                     /gene="pnp"
                     /locus_tag="ACHALG_000866"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFN2586294.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            39522..39788
                     /locus_tag="ACHALG_000867"
     CDS             39522..39788
                     /locus_tag="ACHALG_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFN2586295.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            39932..41923
                     /locus_tag="ACHALG_000868"
     CDS             39932..41923
                     /locus_tag="ACHALG_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HFN2586296.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            42153..43454
                     /locus_tag="ACHALG_000869"
     CDS             42153..43454
                     /locus_tag="ACHALG_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFN2586297.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKIE"
     gene            complement(43584..44954)
                     /locus_tag="ACHALG_000870"
     CDS             complement(43584..44954)
                     /locus_tag="ACHALG_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFN2586298.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(45136..45459)
                     /locus_tag="ACHALG_000871"
     CDS             complement(45136..45459)
                     /locus_tag="ACHALG_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFN2586299.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            complement(45784..46389)
                     /locus_tag="ACHALG_000872"
     CDS             complement(45784..46389)
                     /locus_tag="ACHALG_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFN2586300.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEESTGEGGEPEVIGSTEE"
     gene            46719..47435
                     /locus_tag="ACHALG_000873"
     CDS             46719..47435
                     /locus_tag="ACHALG_000873"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HFN2586301.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            47448..48143
                     /locus_tag="ACHALG_000874"
     CDS             47448..48143
                     /locus_tag="ACHALG_000874"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HFN2586302.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            48227..48856
                     /gene="udk"
                     /locus_tag="ACHALG_000875"
     CDS             48227..48856
                     /gene="udk"
                     /locus_tag="ACHALG_000875"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFN2586303.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
BASE COUNT        16065 a         9045 c        10199 g        14005 t
ORIGIN      
        1 cttttgtttg ttttgagaaa aaaagaagag aaagaaaagt aaggttaaga taacaaatag
       61 taaattttta ttattgtagg atggttgggc tctttttata ataatataaa atataccatt
      121 ttattaataa aaatgcatac tagtaaatga tataattctt ataacaggtt tgatcgcgag
      181 aaaggccaaa taaaacgctc tccaaaatgt taaaaaaaag acaggacgat gtatattata
      241 caaacaagaa tcgctgacaa cagtgactcg gatccaggaa atcagtttta tccccagcta
      301 gttggtgaat gcatggaaag taatatacgt agaaagagct gatgactaat gaattatgag
      361 ttttttttaa gcaaagaaga tcacagaagt tattcattac ttaaatattt agaagcaagc
      421 tcaaatttat cagagtcaat atcgaatgtt caagaagagt tgtcgctatc gacatttctc
      481 ttgaaaaaaa cgatcgataa gctgaaacat gatttacaaa agttcgatct agagaaaaat
      541 ttgcgagtaa ccgtcagtga attggatata tgtctggaaa ttaatggaaa atgttctagc
      601 aaagctctgt tatctcgtta tataacggat tctttatcta tgaaaatggt attggctttc
      661 tttcaggaaa aatatatctc agtagaaaat tttgctaaac agcatcacgt gagttattct
      721 gtggcttata aagtattaca aagactaaaa agaaatctta aaaaatatca aatttttttt
      781 gaaaagagaa agttgacagg gaatcctaaa aacattcggc tttttttata taatcttttt
      841 aactcaacag agataagttt tgtggaattg tatacgctac ctataattat aaaaacagaa
      901 aaaatgttaa aagatttaaa acagtattat cctctgtctg cttatgaaaa aaagaaatta
      961 ttccactttt tagcaatcaa cctgttggct gaacaagctg attctttaga tattgctagc
     1021 aatcagtcgc tatttttttc ggatatagga ttttaccact taaaaggagt attaggagaa
     1081 aaatccgtta tttttatcaa taagacactc tcttggcttt atcttcatga taaattggat
     1141 caaaaatact tgagaaaaaa tgaagatgaa acaatcgaat ggttgaatga acgattcatt
     1201 gaaagttttg aaaatcagtt taatctgttg cgagaagaga cgagccaatt gatccgaaga
     1261 gagttaacca aaatacattt taaacttttg cattacacta ttaataaagc tgatgaattt
     1321 ccacttgatg tttctttttt tagagaaaac tatccagcat tttattgcta tttgatagag
     1381 tatataaatg atctaacgtc aaaacgcaaa gagttgcttc aagataaatt ttttcttttt
     1441 ttcagttatt tattattatt gatcaatcac atacccattc aactgatttc tgaaccagta
     1501 aagatcatca tcgatttttc ttatggtgct gcctataatc aatttatcaa gaaaaattta
     1561 agcttgtaca ttaatctgaa cacggaagtg atagaggcag gaaagcctga gttcccggat
     1621 gttataatca cgaataggaa taatctatat gaagaaagtg catctcaagt agtggtttgg
     1681 ctcgatccgc caagagcagt agactggggg aatcttacaa agattttgtt gactatccaa
     1741 gaagagaaat acaaagacag caaaattgac aagcaaatag acgattttcc tgaagaaatc
     1801 atcgacagga tcgaatagtg aacttttttt agaaagtccg actgcttttt ctaaaaataa
     1861 aaaaacttgg tacgcaatcg ctgtaccaag tttttttatt tagcttttga ttaagccata
     1921 aataataatt tatataagaa tactgctacg acaccagcac agataggtgc tacaactggt
     1981 acccatgcat atccccattg agaagaacct ttatgtttca atggaagaag tgcgtgtaca
     2041 agacgtggag caaagtcgcg ggctgggttt agtccaggac ctgtaggacc accaagtgaa
     2101 gcaactaatg tccatacaag gaaaccgagt gctaagtgag ctgtaccaag attatcttgg
     2161 aagaatggtg cttttgtaat acctagggca ccaaagaata aaacgaatga accaaagaat
     2221 tcattgacga aaccattgaa tttagaacca acgctgttga ttgttgagaa tgtacctaac
     2281 acatggttca cgtcttctgt ttggtcgtag taaggtttgt aacaagctac tacgatcaat
     2341 tgtccaatcg ctgcaccaat caattgtgca atgatataag gaacaacttc agcccaaggg
     2401 aatagaccat tagatgctaa agcaattgta aatgctgggt tgatgtggtt accgctgatt
     2461 ccaccaaaaa tcattgctgg catcataacc ccgaatccat acccgacagc aatcagcatc
     2521 cagtcactgc catatccttt tgtacctttt aaatctacgt ttgctacagc accgttacct
     2581 agaacgatta gtaatgctgt accaatcatt tccgccgcaa gacgggtcat tagtgagtaa
     2641 tccataaata cctccaaaag ttataaaaat gcaaaaaaaa agaaacatca acacgaaaag
     2701 catcggtgca gtgtttcgtg tcggaaggat ttctgcagtc ttaacaaatg tgaataaaaa
     2761 aatgaattcc attacttata agaaaccaga ccaacagtaa aactccaact ctttttaaaa
     2821 gttctccaca aatcaccaag cttaactata aatataccaa aaaaaaaacg ttttttttct
     2881 caaaaattgt atcttagaaa cagcatgttt tgaaactttc agaaaaggtt tgttagtaaa
     2941 atgaaagcta tacgtaagga gtgttgtttg atgaaatttt ttcaagagtt tttcattatt
     3001 attttctttt ctgtattagg agaaggcata cggatttttg ctcatttacc tattcctgga
     3061 agtatcttag gtattctttt tttattttta gcatttgaaa tgcatcttgt tgatcctgaa
     3121 aaaattggag caactggtga ttttttattg aataatctag ctattttatt tgttcctgct
     3181 ggtgtaggtt tattggaata ttttgatgat attgcttcga tatggcctgt tttactggga
     3241 gcagtcgtcg tgtgttccat cgtcacgatg gttgcagcgg gaaaaacggc agaaggtgta
     3301 gaggctttgc tgggctatgt ccgtaagaaa aaagcaggaa gggtatctgt aaacgaaatg
     3361 gaggaaaatc aaattgactg agttttttgt gaatccacta tttggcttaa gtttgacttt
     3421 aggagtgtat gtattatctg ataaattttt gcggaaatat aaactgcctg ttttgaatcc
     3481 tctcgtatta tcgattgttg ttattatctt atttttgcat ttttctggga tcagctataa
     3541 agactataat atcgggggca attttttgac tatgctgatt actcctgcga cagtggcact
     3601 tgccatccct ctttataaaa acttccgttt actgaaagag aatttttatc ctgtaatggc
     3661 tgcgatttta gcaggaatag tcgcaaattg cttggttagt gtagggttcg gttacttctt
     3721 ctctcttcat aaagagatgg tgatctcatt actacctaaa tctgtaacga cggctatttc
     3781 tgtcgattta tctcatacga tgggcggggt gaatgccgtg acacttgcta ttgttgtctc
     3841 tacaggtatt tttggttcgc tgatcgccac tcatattttc cgcctattca agatcgaaag
     3901 tccagttgct cgcggtgtgg cgttaggaag tacttctcat gctatcggta cagcaaaagc
     3961 aatcgagatt ggcgaaatag aaggaatcat ttccggactt gcgatatgcg taaatgggat
     4021 attgaccgtt ttgttgctgc cgttattctt tcagcctttt gctggcttgt tttgatgcga
     4081 aaaccactat tttatgctac aataaccaaa acgttcgagc ataaaaaagg agggaaagac
     4141 gtgtatttgt cttccgagta tcttaaacaa tttgataaag atctatatta taagattctc
     4201 ctcctagaaa gttttgagga tcaagcttgg cataccgctg cacaattagc gcaagttgtt
     4261 cagctggatg cccgcagcgt gtcgaaatac ctgaacgagc tgtctaaaaa ctatcagcag
     4321 tttagcggta agacacaccc tttgtttaca aaaaatcacc gttctggtta taacttttat
     4381 gatacgctcg attcaattga acatgagcgt tttttgatct atctcgtgca aagtacgctg
     4441 aaattccagt tgctgcacga catttttttt gaagaattcc atacaatgta ccaatttgcg
     4501 caaaagcatt acattagtga atctactgct catcgaaaaa tcaatgaatg gaaacagcaa
     4561 ctgcaaactt acggaattcg gctgcaacga ggtacatata tcgctcaagg cgaagaagaa
     4621 atcattcgac tttacttgca tatgacattc tggcagttgt ttcgagggaa aatctggcca
     4681 tttgaaacga tttctcaaat ggacgtaaag aatatggcag aacatatcat ggcttttttt
     4741 aatgttcgtc tgaacgagat caaaaaaaga cgattggaat acatgctagg tgcctttttt
     4801 ttaagaaaat cacagaagca ttatgttgta ttgaatgaga aaaaaagacg gctgatttct
     4861 gacaatctgt tgttccagcg attttgccaa gtcatggagc cggtatttcc taattatttt
     4921 caagtagaag acgagttagg cgcattattt cttgtactga tgactcgtga agaatattat
     4981 agtgatccta agatacggaa gaaaattttt gattttcatc aacaagcaaa aacaccgccc
     5041 tttacagccc tttcagaagc aaaagccgct ctttctcttt atcaggaaga gcaggggctg
     5101 ccagcagaaa atctaacctt cgaggcagaa aactatttat tcagcagtca ttttttcgcc
     5161 tatctttttc ctaatgcgaa agaaacgatt gatggcaata gtagtgattt tattaatcat
     5221 ttagttattg aaaacaagga attgaaacaa tggctcgtac atttttttga aagtagacac
     5281 aaacatccca atcatctggc atttaaaaat catgcgtttt tgatgggtcg gtatctgact
     5341 gtgtttaaaa cattaggagc ctttactcca cagcttccaa agatcacgat ccttttaatg
     5401 acagatttcc cattatttga agagcaactg ttagaggagg gattacggac atttttccgt
     5461 aatgagtatc agctgatttt tctgccaaca gattatcggg ggagagaagt tgatcttctg
     5521 atttcgacaa gtaaagtcca ccgcaaacca tgggcggatc ttgactattt tattgtgacc
     5581 gaagaactaa aattaattga ttacatccaa ctatctcaaa aatttgaaat gattcaaaaa
     5641 aaaactatta aaacaataac aaagaaggag aaaaaagatg acatttgaag aaattttacc
     5701 agaattgaaa aaaggtgcaa agattattcg taaaggatgg agtggatttg aactttatgt
     5761 tgttctcgta tccgatgatg aatttgatgg ctgtccagtg acaccttact tattgatcaa
     5821 aacaagtgac gaaggttttt ccagcttcgc tcctacagtt tgtgatattt tagcagatga
     5881 ttgggcgatc gtagaatgaa aaaccagtat ccagatctag ctaaacaggt tgtatttatc
     5941 acaggagctg cttctggaat tggtgctgcg caagcccgag cgtttcttga acaagatgct
     6001 tttgtatttg gattagatca aaagttcggt aaaatggaag aactctcttt gcgatttcct
     6061 gaaacttttg cctacgcact aggtgatgtt cgacaaatga aagaactcca acaagcggta
     6121 tcgaaatgcc aggatcgttt tggtgaagtt acgattttac tgaatacatc ggggatattg
     6181 gatgcctaca aaccattgat ggaaactgac gaatcgctgt gggatcttat ttatgagacg
     6241 aatgtgaaaa gcatgtatca gctgacaaaa atcatccttc ctgatatgat ccgccgaaaa
     6301 agcggaacaa tcatcaacat ggcttcgatt gcgggtttgg ttgctggcgg aggtgggatc
     6361 gcttatacaa gtgcgaaaca tgcaatcgtc ggatttacca aacaactcgc tctggattac
     6421 gcaggacagg gtatccgagt aaaaggaatt gctccaggag ccatccaaac accaatgaat
     6481 gcggctgatt ttgaagggaa aggtgagatg gctgagtggg tcgcaaatga aacaccagtc
     6541 aagcgatggg cacagccgga agaagtagct gagctgacgt tatttcttgc ctctcctcaa
     6601 gcaagctaca tacaaggaac gatcatccca atcgatggcg gatggttgtt aaaataacct
     6661 tgtgtttttt tcaagcttct gtatgataaa actaaagaga gaaacaacgg ttgatgataa
     6721 aagaaagtcg atgactgttc ccgacattcg atgccgaaca gccagttcaa aaaattcttt
     6781 ttaataatcg ttaatattcg aaactaagcg gcttttcacg accttttgat ataaacggtg
     6841 ttcaaaagct tgcaactgcg gtaacaagtc cagccaaatg aaaaatataa ggagctattt
     6901 ttatttgact attcttatta cttgttgcag ggcagctttt tcactacctt ctgcggcact
     6961 tcagttggaa ttttcttccc atcctttggt cgcagtcatt accaagataa aattatcttg
     7021 caaaggagaa atagaacatg gaaaaaaata atacccgttc agccaaacgc tggacaacga
     7081 cagatgtgac tcggatggcg ctagtcactg gtttatatgt ggcagtgacg ctggttttgt
     7141 ctgtctttag ctttggagtg attcagattc gtctctcgga gatgttcaac tatctgtcct
     7201 tgtataacaa aagatacatc gtcgcggtaa cagcgggagt agctttggct aatatggctt
     7261 ctcctcttgg actgatagat gttgtagtag gaagcgtcag cacgctgctt gtattgctta
     7321 tcaactataa aatcactagt cgcatcaaaa acatgaaaat caaaatggct gtgacagcat
     7381 gcgtatttgc tttttccatg tttacagtag ctggtcagct aacgatttta tatcaactac
     7441 cttttttctt gaattggtgg gtgattgctt taggagaatt ggcatcaatg attgtcggtg
     7501 ggataattat ttattggatc ggtaaaaaag tggatttgtc aaaataaaga tctgtttaat
     7561 ttaaaatatg gcagcgtgga agagactttg agtcttcttc catgctgctt ttttgattaa
     7621 tttgtatgaa gaaatcgatt tagccgtttt ttcatcaaga taccgatata agctgctcca
     7681 gttgccttga ttgcttccgg cagtaaaaac ggcagtagtc cactggcaaa tgcagttgta
     7741 aaagtaagat caccacttat tttcagccag atcgttccgc agatcaatgc agcaagagag
     7801 cccattagat tggcaaagat tgcatataag taatgagcat ccgttctttc gatcatccaa
     7861 ccagttatcc acgtttgtaa gagaaatcca atcaaatatc cgccagttgg cccaaataaa
     7921 acagctatac ctccagtcat ccctgaaaat acaggaagac caatcaagcc aagtaaaaga
     7981 taaactgcaa cagatatcaa tcctagtttt cttccgagaa aagttgctgt taatccaata
     8041 gcaaatgttt gccccgtcaa aggaaccaag ccaaaaggaa tggtcatctg agaaaggaca
     8101 gaaataatcg cggcaaattc cgcagcaagt attaattctt tcaatgttcg gttcataatg
     8161 ccctcttttc gttaacttaa tatttttatt tggttaacga aattgtaaaa caaagtgatt
     8221 actttgtaaa tagaaaatat aaaaaaagac cagctatcca atagatagcg gtctttttca
     8281 agaaagcttt tgaaattata agctttggtt gtagtattca acgacaagag cttcgtcgat
     8341 ttctggatat agttcgtcac gttctggtaa acgagtcaat gaaccttcca atttttcagc
     8401 gtcaaagctt acgaatgctg ggcgtccaac agttgcttct acagcttctt ttactgttac
     8461 gatgttttgt gatttttcac gaactgagat cacttgaccc acttctacgt ggtatgaagg
     8521 gatatcaacg cgtttgccat caactaaaat gtgaccatgg ttaactaatt gacgtgcttg
     8581 acgacgagtc gttgcaagtc ctaaacggta aacaacattg tctaaacgtt gttctagtaa
     8641 gatcatgaag ttaacaccat gtttaccttc tttgattttg ctagctttta cgaacaagtt
     8701 acggaattga cgttcgttca ttccgtacat gtgacgaagt ttttgttttt cagttaattg
     8761 catgccgtat tctgattgtt tgccacggct gttaggtcca tgttgacctg gtgcgtaagg
     8821 gcgacgtgct aattcttttc ctgtgcctga caatgagatg cctaagcgac gagaaatttt
     8881 ccatgatggt cctgtataac gagacattaa aatttcctcc aatgaatata agtttttgga
     8941 gtaaaataat ccgttgaaaa attcataatc atatagtttg ttcttcaatc ttcacctttg
     9001 cagccgcggt tacacaatcg aacccgagac ggggcaacaa actagggatg agttattatt
     9061 tttctgctgc attattttac acaaactaca gtatatgata gatcatccac gaatgtcaag
     9121 ataattagta cataaaaacg ctttacaaac ctaggctgtc tttataaaat gagagggtat
     9181 gtgcaaatgg aaaaaagcga aggaagttta aggagaaaat caatgaatcc ggtatcacca
     9241 ttatttgaga aaatcgatgc ttcaattgaa aagaaggcga atctttttga aaacagcttt
     9301 tcacgttacg ctgttcgagc ggttctcgcc tgtttatttt tgactttagg aacggctgtg
     9361 gcatttgcaa tcgcgatgaa gggggaagat atcgcgcatg gtctaggaaa gattttatac
     9421 gcatttatgt tcagctgggg tctggtcatg attctataca tgaacgctga attaggaaca
     9481 tcgaatatgc tttatatgac cgtgggtgtt tttcggcaga gacttgcttt aagtaaggca
     9541 gccaagattc tatttgcctg tatttttttc aatatgattg gcggtatatt atgtggattt
     9601 cttatttcta tgactacgcc atttcaagat ttaacgttgg acaactttat gttagaatca
     9661 atagcagtaa aactagcaaa acctgcagca acgattttgg tggaagggat gtttgcgaat
     9721 atcgttgtca ataccgctgt actgatcagc atgcggatga aagaggatgc cgggaaagtg
     9781 atcacagtcg tattcatcat tttcattttt gcttttttag gatatgaaca cgtgatagcg
     9841 aattttcctg cctttacgct tgcttacttt gcttctcaag gacagacgga tggaatgaca
     9901 gtcagcaatg tgttacataa tttgtttttt gctttggcag gaaattatat tggtggcggt
     9961 ttagtaatgg gactgggata tgcttggctg aatcagtcaa aatcatccta tgtggactaa
    10021 aaagtcgttc attaaagggg aagtgccatg gaagttttag tttttccaaa aaatgaggaa
    10081 aaagcgttac aaatggaaaa gtacatgaaa aatctttttt cctttgctgg agtacctaag
    10141 ccggaacgcg ctcttttaca acaaccgtta ctggatgaga gcaaaaattt atctatttct
    10201 gaagttttag cttttgttta ttattattat gaacaaatcg aaagagagta tcagtatgta
    10261 gcgatcgatt tagtccagaa aaacatcaaa cggcttacat ttgaagactt agtcgtattg
    10321 ttgccactag ttcagaaaaa agaatggtgg gacagcatcg actcttggag aaaagtatac
    10381 agcacatggt gtaaagccta cccagagcag cgtgaagaag tctttcaatt gtttgaaaaa
    10441 aaggaagatt tttggctacg tcgaatagca atcacgttgc aattaggtta taaacagcaa
    10501 acagacacag gactattgat ccgagcaata gacgaagatc gtcaaacaaa ggaattcttt
    10561 atccaaaaag caattggttg ggcattacgc gattacagta agaccgatgc cgattgggta
    10621 aaaagacagc tgacaacaga tttgagtaag ttagctgttc gagaggcaag taagtattta
    10681 taagaaagcg agacgacaag agatgagacg caaattattt gcttttgata ttgatggaac
    10741 gttgttagat tcaaacaaac aagctttaga tagcacgaga gaagcattgg aaaaattacg
    10801 caaagccggt catctggtca cgattgctac agggcggagc cgatttcatg cgcaagagat
    10861 cattcgcgat cttgatttta cgaactacat tttatgtaat ggagcggccg gttttttaga
    10921 ccatgaacaa gtatataaga atttactaga tcgcgaccag ttggatgcgt tcgttcaaga
    10981 agcgcatcat gagcagatcg atacagcttt tgtcagctta gacaatatca agcgttcttc
    11041 ttccaatgat gtaacggtga tggaagaagc gatgcattct ttcggtgctg tcttgcctga
    11101 attagatcag ttttttgcag agaaacaaga agtataccaa gcgttagcat ttttcgatca
    11161 aacatatgat gggaagttct cttcatatga taaaattcga tttgtccgct ggcatgagaa
    11221 cagtgtagat gtcgtaccta aaaatggttc taaagcagcg acaattctgc acttagcaga
    11281 acaagtaggc atccctcatg aagacatcat cagctttggc gatggacaaa acgaccgtga
    11341 aatgttgcgc atgtcaggaa ttgggatagc tatggggaat gctgtgccag aagtacaagc
    11401 agaagctaag atggtcactg atacaaacga tcaggatgga atttggaagg cattgaaaga
    11461 gttaagcgtg atttaaaaga ggttgtgaaa aagctgttct gcatcaagta ataagcccag
    11521 ccaaacaaaa atagctcctc ctatttttcg tttttctggg cttattaccg cagatgcaag
    11581 cttttgaaca ccgtttaagg aagaggtcgt gacagaagtg gtcagtttca agaaataaga
    11641 agggatttac gaaaattgct tctcaaattt ttgtaaaatt acggcttatt tctgaagaag
    11701 tagatgaaac aacgtttatt cgggaaatgg gactgtgaca agatgtttgt catagtccca
    11761 tttttttatt ttatttttca aaactaagaa ataccaggaa agagttcctt ttgtttgacc
    11821 ttttctttct tattgaaaac aactataata tagagtgatg ttaaaagaga agaggaattt
    11881 agtgtgcggg tattgtttat tggtgatgta gtaggttcaa tgggacgaga gatgattact
    11941 gaatatcttc ctagattgaa gaagaagtac cgtccgcaag taacgattgt aaatggtgag
    12001 aatgccgctt ctggacgtgg aatcacagaa aaaatctata aaaaattttt gcaagacggc
    12061 gtagatgtcg tgacgatggg gaatcatacg tgggacaacc gcggaatctt tgagtttgta
    12121 aatgaagcta aaaagatggt tcgtccggca aattttccgg aagggacgcc tggacaaggg
    12181 atggtctttg tcaaagtgaa ccaagtcgaa ctggcagtga tcaacatgca ggctcgttct
    12241 tttatggtgg acttggatga tccttttcgt aaaatgaatg agctggtaga agaagccaga
    12301 aaacgtacgc cgatcatttt tgtagatttt catggagaaa caacaagtga aaaacaagca
    12361 atgggctggt ttttagatgg gaaagtttca gcagtggtcg gtacacatac acacgttcag
    12421 acaaatgatg cgcgaatttt gccaagagga acagcgtatc tgacagacgt tgggatgaca
    12481 ggtccctatg atggtatttt gggtatgcgc agagaacctg taatcgaaaa gtttctgacc
    12541 gcattaccaa aacgttttga agttgtggaa caaggccgat cgattttaag cggctgtatc
    12601 ttggaattgg atgatacgac aggtcatgca aaagaaatcc agctgattca gatcaacgat
    12661 gatcgaccgt tcatggagta gaaagagagg cagttttttg cagcaggagc cggaaattca
    12721 aaaagaagta aggaaactga caaaactttt aagagagaat gaaacgatca ttcgctacaa
    12781 agaacttgaa gaaaagatcc agcaaaacca gtatttagct gagttgagag aaaaaatcaa
    12841 acaagcgcaa aaagatgctg ttcattttgc ccactatgac aaaccagcag cagaacaaga
    12901 agcaatcaaa caagcggatc aattcatgca agaatttgat cagcatccgc ttgttgtagc
    12961 ttatagaaaa cagctgttgg aagcagatga tctgcttcat catttaacaa ctatgataca
    13021 agaagaaatc aatggacaaa tagaggagga aaaacatgcc tcaaaaaact aaaaatactc
    13081 ctatgatgga acaatatcta agcatcaagg cccaatacca agatgctttt ttgttctatc
    13141 gtttaggaga cttttatgaa ctattttacg aagatgcgat caaagcgtca cagcttttgg
    13201 aattaacgtt gacgagcaga aatcgaaatg cagaagaacc tattcctatg tgcggtgtac
    13261 ctcaccatgc agcacaagga tatatcgatg ccttgattga aaaaggctat aaagtagcga
    13321 tttgcgagca ggtagaagat ccaaagacga ctaaagggat ggtcaagcgc gaagtgatcc
    13381 agctagtcac tcctggtaca gtcatggaca gcaaaggatt gtcggcaaaa gacaacaatt
    13441 ttttaacagc tgtagttagt caaggaaatg aatttggctt tgcttatgcc gatttaagta
    13501 cgggagaatt gaagacggct cggttgcatg atgaagaagc tgttttaaat gaagcctctg
    13561 cattgcagac aaaagaactc gtattaggca gtgagatcac tgataccctt cgtgaacagc
    13621 taagcgggcg tttagggcta gtcttttctc aacaaaatga gatggaagaa aacgcggaat
    13681 ttagcttttt gacaagcgaa ctagtcgatc cgctggaaaa agaagcaacc ggcaaactat
    13741 tgacttattt atcggtgaca caaaagcgga gtttagctca tatccaaaaa gcagtagaat
    13801 accaaccaga tcattttttg aaaatggatt attattcaaa attcaaccta gaattaagcc
    13861 aatctattcg gactgggaaa aagcacggca ctcttctatg gttgttagat gaaacaaaaa
    13921 cagcaatggg tggccgttta ctaaaacaat ggctagaccg tccgcttatc caggcaaaac
    13981 aaattaaagc aagacaagaa atggtcgctt ctttgctaga cagctatttt gaacgtatcg
    14041 atttgcagtc agcattaacg aaagtctatg atctggaacg cctagcaggt cgagtagctt
    14101 tcggtaatgt caatgggcga gacttgatcc agctgaaaac ttctttagaa caagtgccaa
    14161 tgatcagagg gttgattgaa ggaatcgatc aaggtcaatg gcaaagtctg ctttcagaaa
    14221 tgcagccgat ggaccatctc gtccaattga tcgaccaagc tatccaagat gatccgccgt
    14281 tacaaataac ggaaggaaac atcatcaaag atggattcaa tgagcagctc gatatgtacc
    14341 gttctgctat gagaaacgga aagaaatggt tggcagaact tgaagcaaaa gagcgtcaag
    14401 aaacaggaat caaaaactta aaaatcggat ttaaccgcgt tttcggttat tatatagaaa
    14461 tcacaaaagc aaatcttgga aatgcagaac tggagaagta tgaacgaaaa caaactttag
    14521 caaatgcaga gcgtttcatc acaccagaac taaaagaatt ggagacacaa atcctcgaag
    14581 cagaagaaaa atctgttgat ttagaatatc agttgttttt agctgtgaga gaagaagtca
    14641 aaaaggctat ccagccgcta caagtacttg caaaagcaat cagtgcagta gatgtgctac
    14701 aaagttttgc aacgatcagc gaacgctatc agtatgttcg gccagaactt gtcagtgaca
    14761 agcatcagct tctgatcaaa gacggacgac atcctgtagt agaaaaagta ttaggacatc
    14821 aggaatatat ccctaacagc gtagaaatgg cagaagacga aatgatcttg ctgattacag
    14881 gaccgaatat gtctggtaaa agtacgtata tgcgacagtt ggcattaact gttctgatgg
    14941 cgcaaatggg ctgtttcgtt ccggcaaaac aagcagtttt acctattttt gatcggattt
    15001 ttacccgaat tggtgcaagt gacgacttaa ttgctggaca aagtactttt atggtagaga
    15061 tgatggaagc caatcaagcc ctgcgttatg ctacgccaaa cagtttgatt ttgtttgacg
    15121 aactagggcg aggaacagca acctatgacg gaatggcgtt ggcccaagca atcatcgaat
    15181 atatccatcg tcatgtacaa gcaaaaacac tcttttctac tcattatcat gaactgacgg
    15241 tacttgagaa agaacttccg cagcttaaaa atgtccatgt tggagccgtt gaaaaagacg
    15301 gagaagttgt cttcttgcat aaaatgatgg atggacctgc ggataaaagc tacggcatac
    15361 atgtggcaaa gatcgcagga ttgccttcca atctactaga acgagcagca gatatcctgc
    15421 atacgctaga aaatggcgat aacggacacc atactgttcc agctgaagta gaagagaaaa
    15481 ctaaaacagc caaacaggag caaataaaac cacctgcaga aaaagtaaat caagaccata
    15541 tacaagaccg tgtaaaagaa gaaaaccaac agctttcttt attcagtgaa ttatccgaaa
    15601 atgaaacaga agtcattgaa tcactgaagc aaataaattt actagaaatg acgccaatgg
    15661 atgcactaaa cacattatat gaattacaaa aacaactgta ggaagaaggc ggatatcgat
    15721 ggccaaaatc caagaattat ctgaacgttt agctaatcag atcgctgcgg gagaagtagt
    15781 ggagcgacca gcttccgttg tgaaagaatt agtggaaaat gcaattgacg ccggcagtac
    15841 tcagattgat atcttagttg aagaagcggg actgcggacg atccaagtga ttgataacgg
    15901 agaaggcatt ttggcagacg acgtagagaa tgccttcaag cggcatgcga caagtaagat
    15961 ccataatcgc gacgatcttt ttcgcatacg aacgctgggc tttcgagggg aagctttgcc
    16021 aagtattgcc tctgtttctg aaatgactct ggaaacggcg aatcaggaag aaaaacaagg
    16081 cacctatctg tcgctgaaag gcggagaagt tattgagcat cgaccagcac cactgcgtca
    16141 aggaacgaag attaccgtca gcaatctgtt tttcaatacg ccagcccgct tgaaatacgt
    16201 gaaaacactg cacacagaat tagcgaacat cggggacatc gtcaatcgac tggcattgag
    16261 tcatccatcg attgcttttc gtttagtaca tgatggaaac aaaatgctgg caactgctgg
    16321 aaatggcgat ctgaaacaaa caattgcggg aatctacgga ttatctaccg caaaaaaaat
    16381 gctgaaaatc gaaggaaaag atctagattt tgagttattt ggctatgttt cattgcctga
    16441 ggtgactcgt gccagtagga attacttatc gacgatcatc aatggacgtt ttatcaagaa
    16501 cttttccttg aataaagcaa tcgttgcagg ttatgggtca aaattaatgg tcggtcgttt
    16561 ccctattgct gtattggaaa tcaaaatgga cccgctttta gtagatgtaa atgttcatcc
    16621 gacgaagcag gaagtccggc tgtcgaagga aaaagaacta acagagctaa ttagtaacgc
    16681 cattcaaaaa gcattgtctc aagaaaatct gattccaagt gcagcggata atctacggtt
    16741 taagaaaaaa gtacctgatc agcctaaaac agaacaaatc gaaatcgatt tgtcctacgc
    16801 ttttgaagaa gaaaaaccta aaaagccaag tagtttgaat tttgatcgag agacaggtac
    16861 ctttttcgta gaagaaccaa agaacacatt tgttgacaga attgttgata agtctgtgga
    16921 aaatgttgaa aactattctg gtttttctga agaaaacacc acttatccag aaaaaacagc
    16981 gaaaacggtg gataagtttg gtgaaaatgt tgataacttt gtagaaaaaa cagtggaaaa
    17041 atctgaaaag aaagaatttc ccattgtgga taacatggag aaaacaaatg aagaacaagc
    17101 agataaatcc gtagaagagg atgaaaagat ttacgaggaa gaattgacac tccatcctga
    17161 atttgatttc actcaaggaa acgacaaaac attagaacaa gccatcaaga aattagaaac
    17221 ggaaaaacca gtacaacgtt ttcttcagtt ggaatatttt ggacagatgc acggaacgta
    17281 tctttttgcc caaagtagcg atgggttgta catcattgat cagcatgctg cccaagagcg
    17341 cattaagtat gaatatttcc gggaaaaaat tggggaagta tctgatgatc tgcaagaatt
    17401 gcttgttcca tttgtgttag actatccaaa cagtgatgct ttgaaattaa aagagcaaaa
    17461 acacttgcta gaagatgcag ggatttattt agaagaattt ggtcaaaaca gctttattgt
    17521 acgggcccat cctacttggt atcccaatgg agaagaagaa tcgattattc gggagatgat
    17581 cgatatgctg ctgacaactg gaagtgtgag tgtaaaaaaa ttccgtgaag caacagcaat
    17641 catgatgagt tgcaaacgat cgattaaagc caaccattat ttgaatgaag cacaagcacg
    17701 tgtgctgctg aaggatctgg cacgatgcga aaatcctttc aattgtcccc atggccgtcc
    17761 agtattgatt cactttacca attcggatat ggaaaggatg ttcaaacgaa tccaagaccc
    17821 gcattaagtc aaagagaaga gggaaaacat gaagattgta ttagcatccc aatcaccaag
    17881 aagaaaagag ttattggctc atatcgtttc agaattcgag attgaacctg ctgatatcga
    17941 tgaaacgcca tttccagacg aacagcccgt cgattatgtt cgtagaatgg ctgaagaaaa
    18001 agcacgaact gtctgggaac aagatgaaaa agaagaccat ttagtgattg ccagtgacac
    18061 gactgttgtt ctcaatcaag agatcatggg taaaccagaa gatctggctg aggctgaatt
    18121 catgctgaaa aaactaagcg gggaaacaca tactgtatat acagctgttg ttttaaaaac
    18181 aaaggaacga gaagaacgga tactagcaga agcacatgtc actttttatc ctttgacaga
    18241 cgaagagatc aaacgctact tggagacggg ggattatgcg gataaggcag gggcatacgg
    18301 catccaaaat gctgcgagtg tattcgtcaa agaaatttct ggagattatt acagtatcgt
    18361 aggatttccg attggtgctg tcaatcaggc actgaaaaca ttcatttaaa caaaaaagcg
    18421 gactatctga ttttcgattg aaaatccatg atagcccgtt ttttttattg aaaagattac
    18481 ttatctactc tgactaagat tttacttcag ctttaccgac tgcttcgccc gggcgaacaa
    18541 gtcctgattg gtcaagagaa agtcctgcaa ctttttcatt attggtcaaa gcaacaataa
    18601 tgtcagtttt cttgccagct tgttctattt ctggcaaatc catttcagca atcagggtct
    18661 ctggtgtgac aaggtatcct tctttcacga atacattgaa aggaacaccc tttagttcaa
    18721 cagtatctaa tcccatgtga acaagtactt ccagcccctc attagaaaga ataccaatgg
    18781 catgttttgt ttcaaagaca ctcgtcactt taccgtttac tggtgcatag actttgccgt
    18841 ttgacggttc gacagcataa ccgtcaccca tcattttttg tgagaaaact gggtcattta
    18901 cttcagttat ttccttgatg gtccctgcag cgactgcgaa taatgtttct tttgttgcaa
    18961 ctgccgtacc agttgctgaa acaggttcga aagactgtgg cgttgctgtt gcgccatttg
    19021 ctgtttgaag ttgtacatca gcagcatttt caggaacagc aggatcaatc ttattcaaaa
    19081 ttccctgacg tctaaagatg atcgtcaagc caaatggaat gatgacagca atgatcattg
    19141 cgatgatgaa aggtacccat gtttctgctt ggatagctag tattcctggc aaaccgccta
    19201 ctccgatcgc gttagctcga acacccatca agttagcaaa catgccagct aatccagagc
    19261 cgaccattgc agcgacgaaa ggatagacgt atttcaaatt gattccgaac atagcaggtt
    19321 cagttacgcc taagtaacaa gaaatcattg ctggaatcga gatttgttct tcttttttat
    19381 tcccacgatg taagaagatg atggctaaga ctgcagagcc ttgggcgata tttgataaag
    19441 caatcatcgg ccataaattc gttgatccga aatctgcaat cagctgcata tcgatggcat
    19501 tggtcatatg gtggagacca gtaatcacta aaggagcata caggaatccg aatacggcac
    19561 taaacaacca gctgatagaa gacgtcaatc cagtatttac gatatttgag atccagcttc
    19621 cgactgtcca gccgatagga cctaaaataa cgtgtgcagc taaaacagtt ggtaacaatg
    19681 aaaacaatgg aacaaaaatc atcgagatcg actgagggat atatttgcgg aagaagattt
    19741 ctagatacgc taacataaac cctgccagca tggctggaat aacttgcgct tgataaccaa
    19801 tcatctggac ttgagcaaaa ccaaaatccc aaaatggaat atctgctgcg gctgtggaag
    19861 ctacactata agcatttaat aattgcggag aaactaacgt aattcctaaa acgattccta
    19921 atatttgtgt agtacccatc ttttttgcaa tactccaagt gatacctact ggaagaaaat
    19981 ggaaaattgc ttcacccggt aaccacaaaa aggcattgac accattccaa aacttcgagt
    20041 gttcaacgat tgttccacca agactttcaa actggatacc ttcaagtaca tttctgaatc
    20101 caaggatcaa tccaccgaca atgattgcag gaatcagtgg tgtaaagatt tctgctaaaa
    20161 cggcaatagc acgttgaacg ggatgaaggt tttgttttgc agctgctttt ccttgttctt
    20221 tggaaacccc ttcaactcca gatactgcaa cgaagtcgtt ataaaaagta gaaacatcgt
    20281 ttcctatgat gacttggaac tgtcctgcat tcgtgaacat cccttttacg ctaggtatat
    20341 cttcgatcgc tttttcgtcc gcttttcctg gatcattcag gacaaaacgc atacgagtcg
    20401 cacaatgact aactgcagca atattttctt ttccgccgat atcatgtaac agcttctcgg
    20461 catctgcttg atattttgcc atatcaataa acccctttct cttaacttgt ctatgcaagt
    20521 tgtttcttca tttactatac tttttttcgg agagaattgc aagcgtttta ttaaaaagta
    20581 cgagagaaaa tgatatcgtt ttaaaaagct taaaataagc ttttcttaaa aataggaaca
    20641 ttgaaataca aaaagagctg tagtatagtg aagaaatgat ggaagaacga gtgagggaaa
    20701 aaacatgaat aagttcaatg aaattttttt agatttggaa cagaaaattt tagcgaaaga
    20761 atatcctccg cattcgcttt tacccagtga gaaccaattg atcaaaattt atggtgtttc
    20821 acgagaaaca attagaaaag cgctgaatct tttgactacg gaaggttata ttcaaaaaaa
    20881 acaaggaaaa ggttcgatcg tactggatcg gaatcgcttc gactttccta tttctggatt
    20941 gactagctat aaagagctac aggagaccca acgtattcca agtgaaacga tcatccatac
    21001 gttagaagaa acagaagtga caaaagcatt gaacgagatc actggctggg agatcggtgc
    21061 gcctgtttgg catttgatac gcgaaagaaa aatcaatggc gaagtagtta tattagacac
    21121 cgattatcta ttaaaggaaa tcgttcctat tttaactcca gcacaagcac aaggatcgat
    21181 ctatgaatat tttgaaaatg agctttcttt gacgattgct tacgcgcaaa aggagatcac
    21241 agtagaagaa gtaacggaaa tcatgaaaac agcgatggat cttcacgatg acacttatgc
    21301 agtagtagtc agaagcctcg ttcatctgga agatacccgc tgtttcgagt attctgagtc
    21361 ggttcaccga ttagataaat tccgtttcgt tgagtttgcc cgacgccgaa aagtgtaaat
    21421 agttcaaaag aaagcaaatt gttggcatct gtccactttg gcgctatgat aggatcacaa
    21481 agggggcatt tttaatggat aaaccaataa gaccagaaaa agaagaactg aaagaaaagt
    21541 tatcacccat ggcttatgca gtcacacaag aaaatgcaac agaacgacca tttagcggga
    21601 aatatgatga tttttacgaa aaggggatct atgtagatat cgtcagcggt gaaccgttat
    21661 tcagctctgc agaaaaatat gatgcaggtt gtggctggcc atcattcagc aaaccaataa
    21721 cgaaaagaca agtgcgcgaa aaagccgatt tttctcatgg catgcatcga atagaagttc
    21781 gaagcaaaga agcagattct catctcggac atgtgtttac cgatggacca gtagatcaag
    21841 gtggtctacg ttactgcatc aattctgcct cactgaaatt cattccatat gaaaagatgg
    21901 atgaattagg ttatggggaa ttcaaatcac ttgtagaata atggaaatac acaaagagcg
    21961 gtacaatacg gggatttact cgatcgtccg ctcttttttt attcaagcga ttgctatttg
    22021 aaagaaacgc tccccagact ccttttttca caaggtgaaa tgtgctacaa taaagggaac
    22081 gtatgtgtgc aaaagaaaga ggactagaat atgtatgaat atctgacagg tgtggttacc
    22141 tttatcaacc cctattacat agtagtagaa accaatggta tcggctacca gatcgcacta
    22201 ggaaatcctt accgctacag tagcaaacta aatcaaaaaa taaaaattta tgtccatcag
    22261 gtaatacgtg aagatgcgca tcttctgtat ggatttgatt cgctagaaga aaagcaactg
    22321 tttttgcgat tagtaagtgt ttcaggaatc ggtccgaaaa gtgcccttgc aatcatggca
    22381 agcgaagacc actccggttt gattcaagca gtagaatcca gtgatgtcac ttatttgacg
    22441 aaatttccag gagtagggaa aaagacagca caacaaatga tcttggatct aaaaggaaaa
    22501 ttcggtgagc tgagtatcga tacacctttt agtctatttg atgaagcaaa cacaaaagac
    22561 gctacagctc tatctgaagc aatggaagct ctctcggctc ttggttacag tgatcgagaa
    22621 atcaaacgtg tagaaaaaca attaaaagaa gtagataatc agacaacgga tgagtatcta
    22681 cgccaagcac tcaagctaat gatgaaaaaa taaggaggag accaaatgac agatgaatac
    22741 ttgttatcac cagaatctgg cgaaaatgaa ctgagtttgg aaaaatctct tcgaccacag
    22801 ctgctttctc aatatatcgg acagagaaaa gtcaaacaag aacttcagat ttacatcgaa
    22861 gctgccagaa atcgggaaga acctttagat catgttctgt tgtacgggcc tcctggatta
    22921 ggaaaaacga cgatggccat ggtcattgct aatgagatga ccgtaaatat tcgtacgacg
    22981 agtggccctg ctatcgaacg tccgggtgac ttagtagcaa tcttgaatga actggaagca
    23041 ggggatgtcc tgtttatcga tgagatccat cgattaccta gagtggtaga agagatgctt
    23101 tattcagcta tggaagattt ctatgtagat atcatggtag gacaagggcc aaccgcacat
    23161 ccggttcatt tccctcttcc tccctttact ttgattggcg cgacgacacg ggcaggtatg
    23221 ctttctgcac ctttacgaga tcgatttggc atcatttctc atatggaata ttatgacgaa
    23281 caggatctgc aggagatcgt tttacgttct tcggatattt ttcaaacaga gatcattgaa
    23341 gaaggggcat tggaaattgc tcgccgatca cgaggaacgc ctagaatcgc taatcgcttg
    23401 ctcaaacggg ttcgtgattt tgcgcaagtc caaggagacg ggaaaatcga tcgtcctata
    23461 gcggatcagg cacttacttt gctacaggtc gatcaggctg gattggatta tgttgaccag
    23521 aaattgttgc gaacaatgat cgagttatat ggcggcggac cagtagggtt aagtacgctt
    23581 tcagtaaaca taggagaaga gcgtgagacg gtagaagata tgtatgaacc gtatctgatc
    23641 caaaaaggat ttttaaagcg tacacctaga gggcgaattg ctactgctta tgcctatgaa
    23701 cattttggtt acgactatca tatatgattt ttgcttttag attggaggaa aatgaatgag
    23761 tgatacaaca cgtacgaaac aattgatcaa aaacgtattg attgatttgt gccaaacgca
    23821 atcttttcga aaaatcagcg tccaaaaaat cgcaaatgaa gcaaaaatca atcgacaaac
    23881 attttattat cattttacag ataaatacga tttgcttcgt tgggtctatt gtgaggatgc
    23941 attgcattat ttagatccac aatgtctttc acttgataat tgggaagagc aagcattgaa
    24001 aatgctgaaa gcaattgctg agaagaaaga attttatgca aatactgtgc tttctgaccc
    24061 agaaatttta caaaaagagt tttctcaact gatccaaaaa tcatttatca agatttttga
    24121 acaaatggat gaagaaagac aactttctgc aagagacaaa ctattttatg ctcgtttttt
    24181 ttcttacggt tgcagtggtg tattagtcaa ttggatcacg aatgaatatg aagaaacacc
    24241 gatggaaatc gctatgcagc ttttccgttt agccaaggat acggaattat attcttatcg
    24301 tctttatgca aaagaagagt tggaagaaca gtaatagaaa aattttttgt tggaaaagca
    24361 atgatcgcta aaaggagatg gggaaatgac aaaaatttta tttgtttgtt tgggaaatat
    24421 ttgccgttcg ccgatggcag aaggcttgct gagaaaacga atagcattag aaggaaggga
    24481 agcagaattt tttgtggatt ctgctgctac aagcacgtat gaagttggaa agacaccgca
    24541 ccctggaacg aaaaaaatat taaaccaaga aaaagtagat atgacgaata tggttgctcg
    24601 gcaaattaca ccccacgatt ttgaaacttt tgattggatc attggcatgg atcaagaaaa
    24661 tgtagaagaa ctgaaacgaa gagcaccacg ctcagcgcag gggaaaatcc atttgttttt
    24721 aagtagcatt cctggaaaag aaaaagaaaa tgttcccgac ccatactata ccaataactt
    24781 tgatgagaca tatcacttga tcacagaagg cctgaatcat tggtggaata tttggattga
    24841 tgaaaataaa ataacaaaca atttgtaaag ttaacgcttt ctttttaagg cgatgttaga
    24901 gcatttcctt gtttttttgt aaaatgttag gtaaaataaa attaattaag taacactttc
    24961 ttagctaaag gaggagtgca ttcgatgaac gaagagacag tatcgccaat gaagaagctt
    25021 acgaagttag ttactcatac ttcgtatcaa ctttttgatt cttttgtaaa tgacactgaa
    25081 gaacatgcta agaatgtacg attgcaagga aagtttattc ttgagctgca aaaagctgct
    25141 actgaacata gtctagttat cttacaagta agagcaaatc gtttgagtag caagtatgaa
    25201 acgatctcag gctgggtggc gacaaaaacg gtcgaaaaag accgagtgat gatccgccta
    25261 caaaacgaag agcagcagat ccggatggtt cctgtagaac aaattgaaaa aataacaatc
    25321 ctttcaccag caggggaaca ggaacggtta tcaaaataat ttataccaga tagaaaagca
    25381 cgtatgagct tgtaccatca atcattacta atcaaaaata accgaatgct ttaagctgat
    25441 atcagtaaaa cggaacgttt taggatgagt atcatagcat gacagcattc ggttgattag
    25501 ttgaatcgat tggatggtac aagttttttt gataaaaaaa ggtcctgcta gccggggagc
    25561 tagcaggata aggagttaaa aatgaaaaag tgttgttagg gttgtttgtt ggtatgctta
    25621 tataatagtc attagttgtg aatattttgt gacgaaagtt ttcgaaagtt gttaagtttt
    25681 tttgcgaaaa ctatttttat atcatatata tgtctaaaat ctatattata aaagaaatgt
    25741 tgtttgatca gcttctcttc ggtattagtt caaaacaaaa cggcaaagta tatggagcta
    25801 ctttgccgtt ttgtttctct aatactcagg tcagtttata gacgctcaat aaacggtgtt
    25861 caaaagccag tttctcggta ttaagtcaaa tttatgcaaa aatgtgagaa gccattttca
    25921 caaaatattt cttaatactt gaaactaagc ggctttttca cgacctctca ataaacggtg
    25981 ttcaaaagtc agtttctcgg tattaagtca aatttatgca aaaatgtgag aagccatttt
    26041 caaaaaatat ttcttaatac tcgaaactga gcggcttttt cacgacctct caataaacag
    26101 tgttcaagag acagcctatc ggcaataagg ctgaacgtct ctttcacaac ctcttttatc
    26161 aatctgctac ttcttctttc ttagcgaaac tccaaatcag tccgataaca aaaccgataa
    26221 ctgctggtac gatccacccc atccccaatt tgaaaaacgg caagtagctt tccgcaacat
    26281 ataaaagatt ttgaacaata ggtgtttgtt tgatagtttc tgggcaagcg ttcaacccat
    26341 caaaaatcga agcaatcaat gtaaaataag tagtcattcg ataaacagcc gtacgctgct
    26401 taaacaaagg accaactaac actagtaaaa tcagtgtcat tgctaatgga tagatgaaca
    26461 tcaaaacagg caacgaaaat tgaatgatat tcgtcaagcc tacgtttgcc gctaaacaag
    26521 gaaggatact agcaagtgca acaaagaatg cgtaatttct ttgcggaaac aaatctgtga
    26581 atgtttctgc aaaagctgta atcaaaccaa taccggtctt caagcaagct aatataacga
    26641 taaatgctaa taagatactg ccataagtac ctaaataatg atcagcaatc tgagccagcg
    26701 tgatcccgcc attttcactt agagcaaagc tacctaagct catagcacct aaataagcga
    26761 gcaaagtata aatgattccc atcaatataa tgctgatcgc accagatttg atcgtgtctt
    26821 tagcgatttc agcaggtttt gttacaccca tgttttggat tgttgtgaca atgatgatcc
    26881 caaatgctaa agcagcaagc gcatctaatg tgttatatcc ttgagtaaac cctgtgaaaa
    26941 aagcatgctc ttgataattt ggctgaatag gcgcttgatt tacagaacct agtggattca
    27001 aaaaagcaag gagaagcaaa gcacctaata aaaccaaaaa tgcaggattc aggaactttc
    27061 caacataatc taatatcttt gtcggttttc ttgaaaacca ccaagccagc acaaaaaata
    27121 caatactgaa aattgctaga tatagcgtct gtctgctagg atcgataaat ggtgctaaac
    27181 caatctcgaa agatgtggtt gcaaggcgag gtaaagcaaa aaacggtccg ataactaagt
    27241 aaagcaagat ggtaaaaacc atcgcatatg tttgatttat ccgttttgct aattcatata
    27301 cacctgagct tttcgataca ccaatggcaa tcacccctaa gaagggaaga ccgataccgg
    27361 taaccaagaa acctagattc gcccaaaagg tagcagcgcc tgcttcttga cccaaatgaa
    27421 cgggaaagat aagatttcca gcaccaaaaa atagaccgaa aagcatggag cctataaaaa
    27481 gatagtcttt aaatgataat ttttttgtca tacataactc ctttacggtt tttttaaaag
    27541 taacaaaatt tcgaaaaata tgcaacaatt ttcagaaaat tccctatata aaacgatggt
    27601 tatttaataa cgtaagtgat aaaataaaag cgctgaatca tctgaggagg aatcatcgtg
    27661 acaaagtcga gattagaagc atttactgat ggagtggtag ccattgtttt gagagttctt
    27721 gtattagaga tcaagatccc taatacatct agctttcatt ctttatgggt catccgaaat
    27781 acattattag cttataccat cagctttata tttgttgcgg tgatctgggt gaaccatcat
    27841 cgtatatttc agatggcaga acgaattaat taccgagtgg tctggtcgaa tatctttttg
    27901 ctcttttggc tgactttatg tccttctgtt actagttggg tcgggcgtaa ccctgaagct
    27961 ttttggcctg agatggctta tgtcttagta tacacgatgt ggagtttttc ttttggtatc
    28021 ttgatgaggc aaatcatgaa agcaaataaa ccagattccc atgtcgtgag agttttgtca
    28081 aaggaccgtc gaagtcttat ctccatgatg attaacttat gcttgattgc cggtgtcttt
    28141 tttgtaccgt tgatcgggtt attcggacgt ttttttgtat ctggtatttg gattttttcc
    28201 tataaaaaag cagatgccta ttatcagcgg ttgtttccgg ggaaatagaa aagaggctgg
    28261 gacagaagct tttatctcca agaaataagc cgaaatttca caaaaattta agaagcaatt
    28321 ttcgtaaatt ccttcttatt tctcgaagct gaccacttct gtcacaacta ctttcttact
    28381 tcttacgttc atatttcata tgatagatta gtaatagaag caagaaccac acaggtgtta
    28441 gtaataaagc gattctcgta tcttttccta ggaacaagac gacacacaca aaggccatgt
    28501 aaagaaatac cagataatta ctaaatgggt acaacggcat tttaaatgaa tgagcttttt
    28561 tgccgaggat acttttacga tatttcatgt gcgtgtagac aagcatactc cagataaaca
    28621 agaaacaagt agtggcaata ctgcttacta gtgtgaatac ttcactaggc atcacgtaat
    28681 tcaagataac agaaatgaaa atgacgatag tagaaaatgt caaagcattc cctggcacat
    28741 gatgtgttgt taactttttg aattttttag gggcactgcc ttcttgcgct aaagaacgaa
    28801 gcatacgtcc tgtactatat attgcactgt tgcaggcaga agctgctgca cttaatacga
    28861 ctaaattgat caaggaagca gcaatcgtga tcccaatttc cgaaaatact tctacaaatg
    28921 gactgttatc tggattcaag ctgttccatg gatagattgc cataatgaca aaaagagatc
    28981 ctaagtaaaa taagatgatc ctgataggaa tattattgat agcttccggc aacacttttt
    29041 caggattttc tgtttctcca gcgactaatc caactaattc tatcccagca aaagcaaagg
    29101 ttaccatttg gaaagctaag ataaatcctt ttgcaccagt tggaaagaat ccgccatgag
    29161 accatagatt cgtgatactg gctgtgccaa tatctgtcgt atagtgggtg aggatcatat
    29221 aagctcctac gaggataaag gcaatgatcg ctacaacttt gatcaatgca aaccaaaact
    29281 ctaactcacc aaacaatgag acagccacca tattcaagca aagaagaaga cctaatgcaa
    29341 tcagttcagg aagccactga ggaacaccag gcgcccagta gcgtacatac attccgatgg
    29401 ctgttaaatc ggccattgca atcgataccc agcagaacca atacgtccag ccagtgatga
    29461 aagccgcttt tttgccaaga tattctgcaa caaaatccag aaaagaatgg ttattggtgt
    29521 ttgataacag aagttcgcct aacgcacgca taatgaaaaa acagatgcat ccagtaataa
    29581 gataggcgag caatatcgaa gggccagcta gctggataga ctttccggaa cctaaaaaca
    29641 accccgttcc aatcgctccg ccaatagaaa tcagttggac atgtctattt ttcagtccac
    29701 gctgcagttc ttgctcttca ttcattttta aatcatccta actaaaaaag tttaattact
    29761 tccatattaa gagagttttc tgaaaaacac aactaagatt ccaaaatttt cagaaattta
    29821 ccttttatga gtaattcata aaaaagaact gcggtaaaca ccgcagctct ttgtcccaat
    29881 cagctattga tcctaagcat ttaaaatacg tgaaaggaat tcttttgtac gttcttcttt
    29941 cggatggaca aagatatctt ctggtgtacc ttcttctgca atcacacctt tatccataaa
    30001 aatgacacgg tcagatacat cttttgcaaa ttccatctca tgcgtcacga taagcattgt
    30061 caagccagta tgtgctaagt ctttgatcgt attaagcact tcgccaacca tttcagggtc
    30121 taacgccgaa gtaggttcgt cgaatagcaa tacatctgga ttcattgata atgcccgagc
    30181 aattgcgaca cgctgtttct gtccaccaga taattgtgct ggtttggctt cgatataacg
    30241 atccatacca actttttcca gattttcgat tgctactttt ttggcagttt ctttatctcg
    30301 tttcaggaca gttgtttgac cagacataca gttttccaag acattcatat tgttgaacag
    30361 gttgaaggat tggaaaacca tacctagatt cgttcgatat ttaggtaaat tatatttagg
    30421 agctaataca ttttctccat tataaatgat ctcgccgcct gttggttttt ccagtaaatt
    30481 gatacagcgc aagaaagtag atttaccaga accggaagaa ccgatgatcg tcacaacttc
    30541 acctttattg actgttacgc tgatatcttt caatacttca ttttcaccga agctttttct
    30601 tagatgtttg atttcaataa ttttttcatt catgattctt cctccttatt cctgcaaatt
    30661 tgaattgtcg atttcttcaa gcttggtata agctgaagga ccatcgattt tcttttctac
    30721 cgcccgcagg attctggtga tcgtaaaggt catgactaag tagatgatcc cgacgatcgt
    30781 aaatgtttgg aagaattgga aattcgcacc ggaagcagag tttccttgga agaacaaatc
    30841 agctacgccg attacactta aaactgctgt atctttgata ttgataacaa actcatttcc
    30901 tgttgctggc aagatattcc gtaaaacttg tggaataaca acttttctca tcgtttggcc
    30961 atgggtcata ccaatggctt gcgcagcttc gaactgccct tcatctactg caaaaatccc
    31021 cccacgaacg atctctgtca tgtaagctcc agtattgaga gagacaataa acaaagcggc
    31081 aatcgttcgg ttcaatgaaa tgccaaaagc gagtgccaga ccgtagaaga tgaccattgc
    31141 ttgtaccatc atcggtgttc cgcggaatac ttcgacatag atagaaagaa gtgcatttcc
    31201 gattttttgg aagaagcgag tcagtgcatt tcctgattca ggtaccgaac ggaagacacc
    31261 aatcagtaat ccaagtgtag taccgacaac tgtcccgatc aatgcgatga acaaggtaag
    31321 tccagcacct cgtaagaaca tattgccgta ttgtttccaa atcgtagtga aatcagaaat
    31381 gatgccaggt ttttcttcac tatcagttgc agcaggctga tctttgactg cttggtccat
    31441 aattttggtt cgttcatcgt tagagatgcc agaaagaatc tggtttactt tttggatatc
    31501 cgggtcgcct ttacgcatac caacagaaat ctgaacatct tccgggtttg tctggaagcc
    31561 atctgcaccg ctgaattcaa gcatttccaa gtctttgttc acactagttg ccgttacacc
    31621 ttccggacgt tcgctgacat aaccatcgat cattccagaa gctaaagctg ttctcattgc
    31681 tgagaaatta tccattgctt gttgtttctg cacatctggt atctgatcga tcacagtata
    31741 gtggaatgta tttaactgag cagtgatctt tgctccagaa agatctgcta aactttttgc
    31801 ttttgcatat ttgctatctt tttgaactac tactactaat tgtgattcgt aataaggatc
    31861 ggtaaaatca atttcttttc gtcgttcagc agtagggctc attccggcga tgatcgcgtc
    31921 gatcttgcct gattgaaggg caggagccag accatcccat ttcgtttgga cgatgactaa
    31981 ttttttccct aaaccatctg ctactttttt agcgatttga acatcataac cgccggcgta
    32041 actattttgg ccttgaattg gcaaggcatt attctcatct gtttgttgag accagttgaa
    32101 gggtgcatac ccagcctcca tacctacgcg aaactcatcg ctcggtgttt tttcatctgc
    32161 atttgccaga gatcccacac tgaatagtga gaagacaaaa gcaaatagta cggcaactga
    32221 aaccatattt tttttcattt ttttctccct ttctgattat atgatacacg agcggtctag
    32281 atgaaatgcg gtaaagcttt atctgaaaaa ataaaaagcg accgatttat agatataaaa
    32341 aggccgctaa aattccataa ggtgataaac ctctcacaaa acatagcgca acttagcatc
    32401 actgctaaga cagtccgcaa gctcttaact tgcgtcccaa catacactcc aagcaaaaaa
    32461 gtgcacattt cggcgatcct ccctattcct gcttctccat gtttgcttca ctccacaggg
    32521 ttaatgattc tcgcgacctc tacctcattg attgaggcat ctcgtattca gttatttata
    32581 caaaaaaagt ttacgatact cgagaggctt tgtcaatcta taactgctgg tttttgaaaa
    32641 aatactgata aatatagaaa tctttacttt taccgaaaaa acaatcaaaa tgtgaatcat
    32701 ctatccattg caaacgatag tataattata cacaaaaatg tatgtaatac aataaaaaaa
    32761 gaactagaac agaaaatttc ctgtgtccag ttccatctcg atcatttaag ctgccgcttt
    32821 ctttggctca cgtcctagga tcgcttgcaa aagaacaacg acgccataaa tggctaatat
    32881 tagataaatc aaacttgagt attgggtgat tccccagccc catgttcgat atacggctaa
    32941 tgtaatggct aaaacaattg cggcaatcac gttgaataaa gcaaaagccc aaagatttcc
    33001 attttctttg cgtacaagat aaaaaacaga gaaataaacg cttaacgcta accaggcgat
    33061 acaagccaga ataacgcccc aataaataaa gaatggtaac atatcgattc acctcacttt
    33121 tatacagcag tattgtaaca cgttttcatt cttttgtcag tgaacattgt gataaattgc
    33181 tcaaatgccc ataacacttt tttattccca tttttctgac ttttcaacaa taaatttgtt
    33241 cgagtatggg ctataatgga aagtgaggtg acaaaacatg aaacaattaa cagtagacga
    33301 agcagcaaaa gagatattag cttatcagca aattacattt ttgaccggtg caggggtatc
    33361 cacaccttct ggagtgccag actatcgatc tttgtcagga atctaccacg ggatggagca
    33421 gccggaatac ttgctgagtc atcaagcaat ggtgaatgaa ccggataaat tctatcaatt
    33481 tgtcaaaaag atttatcatc ctgaagcaaa acccaatatc attcatttgg aaatagcaca
    33541 tctagcgaaa gaaaaaaatg tttgggttgt ttcccaaaac atcgacggtt tgcacgcaaa
    33601 ggctaaaagc ccgcaactcg tcaattttca tgggagtctg tatcaatgtc attgtcgaaa
    33661 atgtcaacaa acggtagact ggaaagaata tcttcacagc gacaaacatc aagcatgcgg
    33721 aggacaaata cgcccagaca tcgtgttata tggtgaaggg tttcaagatg gagtcatgga
    33781 gcaggcggct tatgccgtca gccaagcaga gctgatcgtc attgttggaa cgagttttca
    33841 agttcatccg ttttgtgatc tggtacagtt tcgccagccg tcagccaaaa tcttagtaat
    33901 caatcaaacg cctgtttatt tagcggagcc ttatacgttt attcaagaag acggagcggt
    33961 tgttttcaaa aaaatacaag aattaggagc agattatgac tgaattcaaa accgaaaaag
    34021 aaaaaatgat tgcaggcgag ctttattttg ccaatgatcc tgagctggtt gccgaccgta
    34081 tgtttgcaag aagtcaaagc caaatcatca accaagcaga atcagcggaa ctgcgatccc
    34141 aattgttgaa agagactttt ggccgtacag ggaaaaaaat ctatatggag ccagttatca
    34201 attttgatta cggatataat atatttgttg gggagaattt ttatgcaaat ttcaattgta
    34261 catttttaga tgtcagcacg attgaaatcg gtgataactg catgttcgct ccgaatgtcc
    34321 agctttacac ggcgacgcat ccactacatc cagtgaaacg taacagcggt ctggagtacg
    34381 caaaacccat taaaatcggt aataatgttt ggttaggcgg aggagttatc gtcacgccgg
    34441 gtgtcactct aggggacaac gtagtagtcg gcgcaggttc ggttgtgacc aaatcttttc
    34501 ccgacaatgt agtgattgca ggaaatcctg ctcgtatcat caagacagta gaggaagaac
    34561 taacagaaga gtctcttgaa acattacgtc ataagatcga tatgatcgat cgagagttgg
    34621 ttgctttatt agaaaaaaga atggatacgg taactaaaat aggacaagtg aaaaaagaag
    34681 aaaaccaagc agtttatgat gcaaaaagag agcagcaagt attggataaa gttgttagtc
    34741 tgctgaaaaa taagggatat aaggaaacaa taacagacac gtatgttgat ttgatgaaac
    34801 actcgcgcga ataccaaaac aaaatgaagg aggaataagt gtgatcgaag gagagacacg
    34861 gcgaaaacaa atcatgcaaa ccttggcaaa tacagaaaaa ccagtgagtg ctagcaagtt
    34921 tgcgaaaact tttggagtca gccgtcagat cattgtaggc gatattgctt tactacgagc
    34981 tgcaggtaaa agtatcgtgg caacagcaag aggttatatc ctcgaaacag aagaaaccaa
    35041 tactgggcac atcagtaaaa tcgctgtcca gcatggaaaa gatcaaacag aagaagaact
    35101 gagactaatc gttgaaaatg gtggtgaaat cattgatgta attgtggaac atccccttta
    35161 cggtgagtta gcaggaactt tgcacatcaa aacgccacaa gatatcgatt cctttatgag
    35221 acgttatcaa aaaagtaatg ctactttgtt atccgaatta acaggcggca tccatctgca
    35281 tacgatccgc tacccagaaa agcatatgct gaaaaacatc aaacaaaaac tagctgaagc
    35341 aggaatcctc tacgaaggat aacatcgtag ctgctacttt ttatcctaga aactattcta
    35401 tcatctctga gataaacacc catatggaag aaataagaaa aaataagaaa agatgcgtat
    35461 ccaatacaca gaattttttg gaattgcgat ttctagatgt ctatctaaat ttcgattagt
    35521 ggagaaacat aaaaaaggga agtgcagctt ttgagcactc ccctttttta taaagctttc
    35581 ttgtagcata gtatcgaagg aacctgccaa ttctctactc gatcaccaat acgccttctt
    35641 ttagtgcaaa tggattttga tcgttgatgt ggtcatagaa catcacaccg ttgatatggt
    35701 cgatttcatg ttgaacgacg atcgattcat aattcttcaa acgaatcttg tgtttttcac
    35761 cattcatatc ataataagac acagtgattt tggcatggcg aacaacatat cctggtactt
    35821 ctcgatcaac agataaacag ccttcacctt cccctaaaca agcatcttga acagaatggc
    35881 tcaaaatttt aggattatac ataacagtac ttagagaagg tccatctgcc tctgggtcag
    35941 gacttggtac gtgaacagca atgattcttt tagaaatatc taattgagga gcagctaagc
    36001 caacaccgcc tcgtaagtgt agttcttcag ctttgattgg atcttggctg ttttttaaga
    36061 actcaagcat ctcttttcct aaagagatat cttcttcgct taatggaaga gaaacttctt
    36121 ttgcaacttc gcgtaaggtc ggatttcctt cacgaatgat atcatccatt gtaatcatgg
    36181 aatatgtacc cctttcttaa aagactgtgt ttcaagtcta ttttcgcatt atagtttacc
    36241 acaaaaatgg aaaagtagta gtaagattgt ttgaaaacta ccttttctta atgattaact
    36301 acttgatttt caacccggtt tcaggtaaag tatagctgtg tgctctaggc acatgacctg
    36361 ccacttggtt aagcgaatca ccaacgtttt actcagtcgc aggggaataa tcgaaagagg
    36421 tgaaagaaat ggcaatttca aaagaacgca agaatgaaat catcaacgaa tacgctcgcc
    36481 atgaagggga tactggttct ccagaagtac aaatcgctgt attaactgaa gaaatcaacc
    36541 acttgaatga acatgcacgc gtgcacaaaa aagaccatca ttcataccgc ggcctaatga
    36601 aaaaagttgg tcaccgccgt aacttgttag cttacttacg taaaactgac gttcaacgtt
    36661 accgcgaatt gatcaaacgt ttaggcttgc gtcgttaatt ttagtcaaaa agagtgggat
    36721 tcacatgaat ctcacttttt ttactcgtat atcaattaag taaaattccg tgatcaagct
    36781 agatggactc ctactaacca aatgaaagct gctcaaaaga cggctttcgg cagttttcat
    36841 ttgtttagta gcgtcttgct ttcacgagaa ggaaaaggag aagttatgtc agaaaaacaa
    36901 gtattcaaaa cgacatgggg cggccgtccc ttacaaatcg aagtcggtca gctagcaaaa
    36961 caagcaaacg gagccgtttt agtccgttat ggagatacag tagtcttaag tgcagctgtg
    37021 gcctctaaag aagcaaaaga tacagacttt ttcccattaa cgatcaatta tgaagaaaaa
    37081 atgtatgcag cagggaaaat ccctggaggt ttcatcaaac gagaaggccg tccaagtacg
    37141 gaagcaacat tgactgcccg tttgatcgac cgtccaattc gcccgatgtt tgcagaaggc
    37201 ttccgtaatg aagtccaagt aacgaacatc gtgatgagtg tagaaacaga ctgctcgcca
    37261 gctatggcag caatgctagg ttcttcttta gcactatcaa tctcagatat cccatttgac
    37321 ggcccaatcg caggcgtaga agttggtcgt gtaaatggcg agtacgtctt gaatccaaca
    37381 gtagaacaag cagaacaaac agatatcgaa ttgactgttg ccggaacgaa acaagccatc
    37441 aatatggttg aaagcggcgc gaaagaagtt tcagaagaag acatgcttgg tgcattgctg
    37501 tttggttttg acgctatcaa agagttagtt gctttccaag aagaaatcgt tcaagcagta
    37561 gggaaagaaa aaatggaagt gactttactg caagttgatg aagtattgaa aaaagaaatc
    37621 tttgatgctt cttacgctac aatgaaagca gcagtaatga ctgaagaaaa acttgctcgc
    37681 gaagataata tcgagcaggt gaagatcgat atccgcgaag cttatgctga gaaatttgca
    37741 ggtcatgaag atgaagatca cttgttgaaa gaagttaaac aaatcacaga agatcttgaa
    37801 aaagatgtcg tacgtgaatt gatcaccatc gataagatcc gtcctgacgg gcgtaaattg
    37861 gatgaaatcc gtccattgtc atcagaggtc agcttattgc cacgtgtaca tggatcaggt
    37921 ttgttcacac gaggacaaac acaagcttta tcagcttgta cactcgcacc tctaggagag
    37981 caccaaatca ttgatggttt aggggtggaa gtcagcaaac gattcatcca ccactacaat
    38041 ttccctcaat tttctgttgg ttctacagga cgtgcaggct ctccagggcg tcgggaaatc
    38101 ggacatggcg cattaggtga acgtgcgtta gcacaagtaa tcccttcaga agaagaattt
    38161 ccttatacga tccgtttagt agcagaagta ttggaatcaa atggttcttc ttcccaagca
    38221 agtatctgtg ctggcacatt agcattgatg gatgcaggtg taccaatcaa agcgccagtc
    38281 gccggtattg cgatgggact tgtttctgat ggtgaaaact atacgatcct aactgatatc
    38341 caagggctgg aagatcatct tggcgatatg gactttaaag ttgccggtac aaaagatggt
    38401 atcactgctt tacagatgga tatcaagatt caaggaatca cagaacaaat cctaactgaa
    38461 gcattaactc aagcaaaaca agcacgtatg gaaatcttag aagaattgac ttcaacgatt
    38521 gctgctccac gtgaagaatt aagccaatat gcaccaaaaa tcgaaatgat ccagatcgaa
    38581 cctgctaaaa tcaaagatgt tatcggtaaa ggcggcgata cgatcaacgg aatcatcgat
    38641 gaaactggtg tgaaaatcga tatcgatcaa gatggaaaag taagtattgc ttcagctgat
    38701 gcagaaatga tcaaaaaagc tatcaagatc atcgaagatt tgaccaaaga agtcaaagtt
    38761 ggtgaagttt atctagggaa agttgtccgt atcgaaaaat tcggtgcatt cgtcaacttg
    38821 atcaaaggaa aagatggact tgtccatatt tcccaattag caaacgatcg tgtcaataaa
    38881 gtcgaagatg ttgttaaact aggtgatgag atccttgtga aagtaactga aatcgataaa
    38941 caaggacgcg taaatctgtc aagaaaagca atgttgaacg aagatggttc aacgaaaaat
    39001 gaagaaaaat aatttaattc ataggaataa tgtacttgtg atcgtgaagg tgacaggggg
    39061 acaataatta ttcgaaaaaa ggggactgtg acaaaagccg tgtcacagtc cttttttttc
    39121 gaataaacgg tgttcaaaag cttgcatctg cggtaataag tccagccaag ttaaaaacat
    39181 taggagctgt ttttacttgg ctgttcttat tacttgatgc agaacagctt ttttacaacc
    39241 tctccaataa acgatgttca atcagctgtc cctcgatatt agctcaaaaa acggcaaaat
    39301 atgaggaact atttttccgt ttgtttctct aatactcagg tcaaaccgct gatttcacaa
    39361 cctctgattc acggtgttcc agaagcagct tctacggaaa taagctgaac acttttgtta
    39421 caacttcttt ttttctggag tttcttggcg catatttttg gagtccttct gaataaagcg
    39481 taaaataaaa gtgagaaata tggataaagg agggaaagga gatgctgaat tttttatggt
    39541 cgttatttat cggcggtata ttaggtttga tagctggcgc aattttagga aaggaagtcc
    39601 cgggaggcgt atctggaaat atcattatcg gttttttagg tagttggctc ggcgaatttt
    39661 tactaggacc actgggaccg gaactcggcg gtttttattt gataccagca ttattgggtg
    39721 caattttttg ccttgctctt tattcatttg tggcaagacg tatcagagag cgtccaaaga
    39781 aagaataatt ttattcaaat aagttgacac ttcagtgtgt tacttggata attagaagca
    39841 atttaggaaa gcgggtgtca tggataagaa tatcatctat cattcaagct tacctaaagg
    39901 atatccatat aacaagaaat gagggaaaaa cgtggcaaca acgcacaata ccaaaaagat
    39961 atcaatggct ggcctgcttg tagcgatggg agtcgtctac ggagacatcg ggacaagtcc
    40021 gctttacgtt atgaaatcga ttgttgaaga taatggcgga ttgtcaggaa tcaatcctga
    40081 ttttgttatc ggatcagtct cattgatttt ttggacattg actctactga caacaatcaa
    40141 atatgtcgtg attgcattga atgcagataa ccatggtgaa ggtggaattt tctcactcta
    40201 cacacttgtc cgaaaaggtg ggaaatattt gatcattcct gcaatgatcg gaggagctgc
    40261 cttactagca gatggggtat tgacccctgc tgtgacggta acgactgcga ttgaaggctt
    40321 gcgaggaatt ccgcagtttt ttgaccggtt cggtaataac cagaacgtga tcgtcattat
    40381 cactttagtt attatttttg ttttattcat ggtccaacgc tttggtacag atattgtcgg
    40441 aaaagcattc ggaccaatca tgttcgcttg gtttactttt ttaggcgtaa tgggattaat
    40501 gaacttcagt caagactgga cagttatccg tgcactgaat ccttattatg ctatccagct
    40561 gctttttagt gcagataaca aattaggctt gttcattctt ggaaatattt tcctggctac
    40621 gacaggtgca gaggcgcttt attccgatct tggacatgtc ggcaaacgaa atattcgtct
    40681 gagctggcct tacgtcaaaa tttgtttggt tatcaattat tttgggcagg ctgcgtggtt
    40741 gttgaatgtt tatcaaaacc ctgaagcaca gcaaatcaag aacctgaatc cattcttcca
    40801 aatgatgcct caaagttgga tcgtgatcgg cgttgctttt gcaacagtcg ctgcagtcat
    40861 tgcttcgcaa gcattgatta caggttcgtt tacgctagtt tcagaagcaa tcaagttgaa
    40921 actgctgcct cggttaaaga tcatgtatcc tgggaacagt atcgggcaaa tgtatatccc
    40981 agcagtcaat ctgattttat ggatcgcctg ttctctagtg gttgtcacat tccgaacgtc
    41041 tcatcatatg gaagctgctt acggtctatc gatcactgtg acgatgttga tgacaacagc
    41101 actgctttac ttttatctta tccaaaatgg gcattctcgt tggcttgcat atttagttac
    41161 ctttttcttc ggtgcgatag aagtcgtttt ctttatctca agtgttgtca aattcttccg
    41221 aggcggtttt gtagccgttc tgattggttt gatcatctta gtcgtgatgt ttatttggga
    41281 acaagggaac attattcgcg aatcgtcaac cgaggatgtg tctttgagag attatatccc
    41341 gcagcttaga gaattacgtg atgatacttc tttaccaatc tatcagacaa atgtggtctt
    41401 cttggttcct gatatggctg aagataaagt tggccgacag ttcatctatt cgattttaga
    41461 taaacggcct aaaagagcac gagtctattg gtttgttcat gtggaagtga cggatgagcc
    41521 atatacgaaa gaatatcaag tagatatgat ggacactgat tttattgtcc aagtgaatct
    41581 gttcttaggt ttcagggtcc aacaggaaat caacgtatat gttcgccaag tcatccatga
    41641 tttgatgaaa caaggccgtt tgccaaaaca gccacaaaga tactctctga caccagggcg
    41701 agaagttggc gatttccaat ttgtcctgat tgatgaagta gtttccaatg tgacaactct
    41761 aggaaaatgg caacgtcaaa tcatgcaggc aaaactggca atcaaaaaaa tcgctacctc
    41821 gccagaaaca tggtttgggc tagagtacag cgaagtaaaa catgaaactg ttccgcttat
    41881 cattggaaat actcggaaaa catggttgaa agagcggttg tgaaagtagc gctttgacct
    41941 gaactaatgt cgaatgtcag ctacttgaac accgtttatc agaggtcatg aaaaagctgt
    42001 ttagcaacaa gtaacgcaat gattttgaag tgataaggag atgtggctga aaaatgccgc
    42061 atctcctttt ttattcctaa atatgaatac gcattatttt caatcttttt gtagaaaaag
    42121 ggtacaatag aaaaagagaa aggcgggaga atatggttat caatttcatt attttatttt
    42181 tgatgattgc aattacttca ttttttgtag caagtgaatt tgctttagtg aaaatcagac
    42241 gatcaagatt ggaacaacta gaaaaggaaa atgtaaagaa cgccaagcta gctcttcatg
    42301 ttacacacca tttagataac tatttatcag ctagtcaatt aggaattacg ctaaccggtt
    42361 tgatcatcgg gtgggtcggt gaaggatctg tcgcggcttt actagaacca gtgatcggta
    42421 aactaccttt tagttcggct attagcagta caatctctgt ggcgctggga tttattttag
    42481 ttacgtatat cgatgtggtt attggcgaac tgcttcctaa gagctatagt atcgtcaata
    42541 cggagaaggt cgtcttgttc gtagttaaac cacttcatta tttttataaa gtaatgtttc
    42601 cttttatttg ggtattgaat cattcagctg cagggctagg gaagctatta ggtgtgcgtc
    42661 tcgtatctga aggagaagag acattgtctc aagaagagct gacattagtc gcattgaatt
    42721 cttatgaaaa aggagagttg accaaagaag aatatcatta tttggaaaac gtgtttgaat
    42781 ttgatgatac cttggcaaaa gatatacaag tagaccgtac gtccatgcaa gtattcgaag
    42841 cttcagaaac cgtcaaagaa gcgataaaag cttcgattga acaaggacat actcgctatc
    42901 cggttatctt agaatcaaaa gacaatgttt taggttatgt caccttgcca gatttgatca
    42961 agcaatctta taaagatgac cagctgacag tagaacagtt gattgaagag ccgatcgtaa
    43021 caactgaaac gatccctata aaaaaattat tgacgatcat gcgaaaaaag ggaaagcata
    43081 ttgctatctt aaaagatgaa tacggaggga caagcggctt agtgacgatt gaagatattt
    43141 tagaagaaat cgtcggggaa atccgagatg agacagattt ggacgaagca ttgatagcgg
    43201 aacagtcaga tggctcttat atcatctccg gcaaactaac attagatgat ttccagcgtt
    43261 attttcatgt agagattcca gagtttgaag aaacgaattt tactacactt gcgggctttg
    43321 cttctagccg atataaagaa ataaaagcag gaacaatcat tgagattgca tcattccgtt
    43381 tcacggtatt agaataccag catgcacata tcgattattt caaagtagag tccacggaaa
    43441 gaaaaataga ataaaagaaa aaaggctgtg acattttttg tcacagcctt tttgttatct
    43501 aaaaaacctg ttttcggtaa acgaagaaga tattttcaat aaaagttttc atttttagcc
    43561 gcaacgattt cttttaatca aggctatctt gcttctgcat gttctaatgt gaaatgttcc
    43621 tcttcaccca tgtgtttcaa ttgtttcatc gaacgcatat aagagtaaag caacacggca
    43681 acagaaccga tccaagctaa aggagaagct gctgctgctc ctgggtatcc ccacattcct
    43741 gtaagaacga tagctgcaaa agaacgcata agcaattcca tcataccggc gatcgtaggg
    43801 atcttgcttt gtccaagccc ttgaagtgtg tagcgtaaaa tgaacaggac tgctaagatc
    43861 cagtaaagag acccattgat attgaagtag gtttgtgcta attcaaatac acgtgtttct
    43921 gatgggctaa caaacaattg aacaaagaaa tgaccgaaga aaatgacgat agcacctgct
    43981 aagagactaa atccgacact catcagtaaa gtttgacgta ccccttgcaa gattcggcca
    44041 tattctttcg caccatagtt ttgtgcagtg aaagttgcca ttgcaatacc aaatgacatc
    44101 atcggctgat ttgcaaattg gtcgatacga ccagcagcgg cttgagcagc tacgacatct
    44161 gtccctagac tatttagtgc tgcttgtaac acgatagcac caacagcgat gatcgaagat
    44221 tgaaacgcca ttggcaaagc agcatttagg tgtacagcaa actcttcttt ttcaaatttg
    44281 aaatcttttt tgcgtaactg taacaaaggc atttttttct tgatatacac tagacacagt
    44341 acgctggaag aaacttgtgc gattactgta gcaatagctg caccttccac acccatgcca
    44401 aaatagataa taaagatcaa atcaagtacg acgttgatga tgaccgcaat gatcagaaag
    44461 aatagtggtg tgcggctgtc accaagtgcg cgaatgacat tagaaagcaa attgaaactg
    44521 actgatgcaa agatccctaa taaaataatt gaaataaatg tttgtgcttg atcaagtatt
    44581 tcaggaggag tttgcatcaa ttgaagcatc ggacgaataa acaggagact taggacagtt
    44641 aaaatcacag tgacgacaag actaatgaca acactagcag caaaactttt cttcagaccc
    44701 cgataatctt ttgcaccgta acgttgtgcg gtaataatgg ctaaaccagc tgttaaacct
    44761 tgggcaaagc caatgatcaa aaatgtcagg cttccagttg caccaaccgc tgccagcgca
    44821 tttttaccta gcgtctgacc cacgatgatc atatcgatca tattgtagaa ctgctgaaaa
    44881 acatttccga tcagtaaagg aacagtgaac atcaaaatca gtttagcggg gcttcctttt
    44941 gttaaatcgc gcatctttta aactccttat atctttaaaa tttagttagt agtatatagc
    45001 gtttatcctg agaataccaa ttagaaatca atgataattt atgaaactcc ataaaaagaa
    45061 gccaagaatt gagtcaaaga atcaacaact tggcctggtc tgtaaaataa gggacatcca
    45121 cgtttatcta tcaccttaat attgtctaat ctcacttggt aaaaggatcg tttctctcgt
    45181 accgtcatca taggcgtata cttgagcagg aaattggttc gaataggcaa acggcaaatc
    45241 aatatccatt tccacattgc ttcctgcttg agaaaaatgc atcgtcactt ttccacgatt
    45301 ttcgatcaaa tcaaaggtta atatattttc taatggaatg actcccttaa gatctaggtc
    45361 aataataaac catatactgt caatcaattc atctggcaaa cttgatacga caccaactga
    45421 agcgtaacgg ttgcggctgc tatcaaaact ttcaaacatc tgtttaacct ccttcgaatc
    45481 aacgaatgtt ttatttttat aattaatcct aggataaact agaaatactt acttcaacga
    45541 gggttcattc taataaagaa tcaggaaaaa tgcaacaata attgaatact ttttttcaaa
    45601 gtataaattt ttataaaggt acgataaaag tatgcgagga aaagcttggt aaattaggat
    45661 ttttcgtttg taaagccatt tacaaaagtg gaagccccat atgaaaataa acgattagaa
    45721 ataaaaaagg cgtgttaaaa gctgctgacc cgccgcttct aacaccacct gattgtttca
    45781 gtattattct tctgttgaac cgatcacttc tggttcgcca ccttcacctg tgctttcttc
    45841 ttcactttga gcgactacag cagcgatttg ctcttcactg tcggtcacga atttgtaatc
    45901 gtcgtttttt ggaagatcgg ctactgtaat agaatcacct aattccaagt ttgtcacgtc
    45961 aacttcaatt cgttcaggca atttgtctgg tgtagcagat acgactactg tatacaagtt
    46021 ttgagctaac actccaccag atttagcacc cactgattcg ccaacaagaa cgacttctgt
    46081 ttcgacttct gtttcttccg acatgtttac agatagaaat tctgcatgtt cgaaatgatt
    46141 ggtaaaagtg ttcgtttgga tcttgtgaac caatgtattg actttttgtc catcgatgtc
    46201 catcgtgatg actgtgttcg ctccatgttc acgcaacaat ttgcttagtg tcaaaccatc
    46261 gaatgagata ggggtacttt ctactttata cccataaaca acagctggaa ctttcccttc
    46321 gtgacgaagt ttgtttctta atgaacgggg acgtacttct cttttactta cttctaatga
    46381 tactgacata accaaaattc ctccttaaaa tgtgactgtc tgctttggta acggacagat
    46441 tttatttaag tgaattctaa aggctaatct cgtgcacatc atcgaaggca gggcgcttaa
    46501 aaaagcgcaa aaggagagat gcgcgcatct ccccttagaa agtcaggatt caacttttct
    46561 ccaccaggtc attacgctgt gtatgagaaa tccaattcac tttactattt aaccgtaacg
    46621 tgaactgtta attaagtcaa ggaataacta tgatgatgta tttgcaagca agaatattca
    46681 aggtataatt aattagtagt aataagaaag gaaacagcat gcgtttagat aaattaatag
    46741 aagaaaaatt agaaacgaca cgcaaggaaa tgaagcggct gtttgctttg aagcaagtga
    46801 tgatagatgg cacagtcgaa tttcgacaaa atcgaaatgt agatagttta ctgcatcaaa
    46861 tcgaagtagc aggtgaacac ttagaaacaa atgagtccta ttatcttctc aataaacctg
    46921 aaaaagctgt tacagctgta tcagatccag aaaagacgac agtaatcgat ctgattgcaa
    46981 gtgccgatcg tttccaacca ttatatccag tgggaagact agatagagat acaacaggac
    47041 tattgctgat cacttcgaat ggacaattgg gatatgaaat gctacttcca gaaaaaaagg
    47101 tttcaaagac ttatcaagta gttgtgaacg aaagggtgac accggcagac gtaacagcat
    47161 ttgaaaatgg aatcgtattc gctgatgggt atcaatgtaa gccggctgat ttaaaaatac
    47221 tcaaagcaga caagcaccaa agtacggttt tattgacgat ccaagaagga aaattccatc
    47281 aagtcaaaaa gatgttctta gcccaaggaa agaaagtgac cgcattgaaa cggctaagca
    47341 tgggaccatt ggaattagat gaacagctag gagtaggcga gtatcgatcg ttgacattag
    47401 cagagatgaa aaagttaagt atttatttta aataggaggt caaaaagatg aaatataaaa
    47461 ctttattgtt cgatgtagat gatacattgt tagattttca attaactgaa gccaatgctt
    47521 taagtgcttt gtttgcagaa caaggaatca cattgacacc agaaatcgaa accagctata
    47581 aggctttgaa tcatcattta tggcaggagt ttgaaaaagg cagactatca cgagaagaaa
    47641 taataggcag tcgttttggg ttacttttcc agcaatttgg gaaaacagta gatagtgatc
    47701 aaatggaaaa acgctaccgc cactacttga accaaggtca tgatcttgtt ccaggaagtc
    47761 tagaaatcct gaaaaaggtc catccgcatg ctgatcttta tattgtgacg aacggtgtgt
    47821 cgcacacgca acatcggcga ttaacagatg caggaatgct tgattacttc aaggatatct
    47881 ttgtctcaga agctgtcgga gcgcaaaagc cgatgaaaga attttttgat catgtctttt
    47941 cccacattcc taattttgat aaaaagcaaa ctgtgatcat tggtgattct ttgacttctg
    48001 atatcaaagg aggaaatatg gcagggatcg atacgatttg gttcaataga aataaattac
    48061 cagaaatcga agaaatccag cctacttatc gaatcgattc attagaggaa ctttattcat
    48121 tattggaaat tgaaaaggat taattttcag aaaaatatag cattttcgtg ttttttcgct
    48181 ataatagatt agaatgaaaa aacggatacg aaagaggttt tagtcaatga caaaaagcaa
    48241 gccaattatc attggtgtga cgggtggatc cggaagcgga aagacaagtg tcagccgtgc
    48301 gattttcaac aacttcccgg accattccat catgatgctt gaacaagatt cttattacaa
    48361 ggatcagagc catttgagtt ttgaagaacg attgaacaca aattacgatc acccatttgc
    48421 ctttgataat gatctgctga ttcagcatgt aggcgactta ctgaactata aagccattga
    48481 aaaacccgtg tatgactatg tagcgcatac aagaagtcag gcaacgatca ttcaggaacc
    48541 aaaagaagtg atcattctag aaggaatcct gattttagaa gacgaacgtc tgcgtgactt
    48601 gatggatatc aaagtatacg tagatacaga tgatgacatt cgtatcattc gtcgtatcaa
    48661 acgggatatg gaagaacggg gacgcacatt ggactccgtg atcgaacaat atttgacagt
    48721 tgttaaaccg atgtatcatc aatttatcga accaacgaag cgctatgcgg atatcattgt
    48781 tccagaaggc ggagaaaacc acgttgctat tgatttgatt acaacaaaag tagctagttt
    48841 cttaaaccat aaatagtgcg cagcgaaaca gttgaacgga ctgcgaccgt ttgaaaagca
    48901 aggattctgc tttttgaaca gtcaccagcc aactgttttt ttatttataa caagaggttg
    48961 tgaaaacagc atcttgacct aagtattaaa aagaagaaac ggaaaatagc tgtttatatt
    49021 ttaccgtttt ttgagctaat accgaaggtc agctgattga gcaccgtgaa gtagaggttg
    49081 taaaaaagct gtctttgcat caagcgataa gagcatccaa ataaaacagc tcctcacgtt
    49141 tttcgttggg ttgggcttat tatcgtagat gcaagctttt gaacaccgtt taacgagagg
    49201 ttgtaacaga agtggtcaga tgcaataaat aagaagaaac ttacgaaaat tatttctcga
    49261 atttgtgtga gatttcgact tatttttttg aagaagctct tttgtcgcac cccc
//
LOCUS       DAZABD010000014        48116 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000014 DAZABD010000000
VERSION     DAZABD010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 48116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48116
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.014"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(38..1189)
                     /locus_tag="ACHALG_000876"
     CDS             complement(38..1189)
                     /locus_tag="ACHALG_000876"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFN2586304.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(1202..1639)
                     /locus_tag="ACHALG_000877"
     CDS             complement(1202..1639)
                     /locus_tag="ACHALG_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFN2586305.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            complement(1654..3420)
                     /locus_tag="ACHALG_000878"
     CDS             complement(1654..3420)
                     /locus_tag="ACHALG_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol-specific transporter subunit IIBC"
                     /protein_id="HFN2586306.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            3684..4361
                     /locus_tag="ACHALG_000879"
     CDS             3684..4361
                     /locus_tag="ACHALG_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFN2586307.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            4364..5491
                     /locus_tag="ACHALG_000880"
     CDS             4364..5491
                     /locus_tag="ACHALG_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN2586308.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(5691..6131)
                     /locus_tag="ACHALG_000881"
     CDS             complement(5691..6131)
                     /locus_tag="ACHALG_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFN2586309.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(6142..8175)
                     /locus_tag="ACHALG_000882"
     CDS             complement(6142..8175)
                     /locus_tag="ACHALG_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HFN2586310.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(8177..9880)
                     /locus_tag="ACHALG_000883"
     CDS             complement(8177..9880)
                     /locus_tag="ACHALG_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFN2586311.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(9974..10924)
                     /gene="manA"
                     /locus_tag="ACHALG_000884"
     CDS             complement(9974..10924)
                     /gene="manA"
                     /locus_tag="ACHALG_000884"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HFN2586312.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            complement(10963..11988)
                     /locus_tag="ACHALG_000885"
     CDS             complement(10963..11988)
                     /locus_tag="ACHALG_000885"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFN2586313.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            complement(12197..13066)
                     /locus_tag="ACHALG_000886"
     CDS             complement(12197..13066)
                     /locus_tag="ACHALG_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFN2586314.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(13770..15662)
                     /locus_tag="ACHALG_000887"
     CDS             complement(13770..15662)
                     /locus_tag="ACHALG_000887"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN2586315.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(15659..17386)
                     /locus_tag="ACHALG_000888"
     CDS             complement(15659..17386)
                     /locus_tag="ACHALG_000888"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN2586316.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(17402..18052)
                     /locus_tag="ACHALG_000889"
     CDS             complement(17402..18052)
                     /locus_tag="ACHALG_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HFN2586317.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            18256..18552
                     /locus_tag="ACHALG_000890"
     CDS             18256..18552
                     /locus_tag="ACHALG_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFN2586318.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            18744..19364
                     /locus_tag="ACHALG_000891"
     CDS             18744..19364
                     /locus_tag="ACHALG_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFN2586319.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(19456..20622)
                     /locus_tag="ACHALG_000892"
     CDS             complement(19456..20622)
                     /locus_tag="ACHALG_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFN2586320.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(20634..20987)
                     /locus_tag="ACHALG_000893"
     CDS             complement(20634..20987)
                     /locus_tag="ACHALG_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFN2586321.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            21115..22137
                     /locus_tag="ACHALG_000894"
     CDS             21115..22137
                     /locus_tag="ACHALG_000894"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HFN2586322.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            22156..22317
                     /locus_tag="ACHALG_000895"
     CDS             22156..22317
                     /locus_tag="ACHALG_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2586323.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(22576..23010)
                     /locus_tag="ACHALG_000896"
     CDS             complement(22576..23010)
                     /locus_tag="ACHALG_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2586324.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(23120..24979)
                     /locus_tag="ACHALG_000897"
     CDS             complement(23120..24979)
                     /locus_tag="ACHALG_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFN2586325.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            24980..25120
                     /locus_tag="ACHALG_000898"
     CDS             24980..25120
                     /locus_tag="ACHALG_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HFN2586326.1"
                     /translation="MLAPSFLSIKECCFSYFSCTKFESKERKEELMKSKYSLSLWPFW
                     FL"
     gene            complement(25097..26256)
                     /locus_tag="ACHALG_000899"
                     /pseudo
     CDS             complement(25097..26256)
                     /locus_tag="ACHALG_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
     gene            complement(26563..27987)
                     /gene="araA"
                     /locus_tag="ACHALG_000900"
     CDS             complement(26563..27987)
                     /gene="araA"
                     /locus_tag="ACHALG_000900"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFN2586327.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(28003..28701)
                     /locus_tag="ACHALG_000901"
     CDS             complement(28003..28701)
                     /locus_tag="ACHALG_000901"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFN2586328.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(28658..30319)
                     /locus_tag="ACHALG_000902"
     CDS             complement(28658..30319)
                     /locus_tag="ACHALG_000902"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFN2586329.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            30537..31937
                     /locus_tag="ACHALG_000903"
     CDS             30537..31937
                     /locus_tag="ACHALG_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HFN2586330.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(32189..33268)
                     /locus_tag="ACHALG_000904"
     CDS             complement(32189..33268)
                     /locus_tag="ACHALG_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFN2586331.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(33354..34313)
                     /locus_tag="ACHALG_000905"
     CDS             complement(33354..34313)
                     /locus_tag="ACHALG_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFN2586332.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(34325..35836)
                     /locus_tag="ACHALG_000906"
     CDS             complement(34325..35836)
                     /locus_tag="ACHALG_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HFN2586333.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(35858..36421)
                     /locus_tag="ACHALG_000907"
     CDS             complement(35858..36421)
                     /locus_tag="ACHALG_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFN2586334.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(36427..37290)
                     /locus_tag="ACHALG_000908"
     CDS             complement(36427..37290)
                     /locus_tag="ACHALG_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN2586335.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(37304..38200)
                     /locus_tag="ACHALG_000909"
     CDS             complement(37304..38200)
                     /locus_tag="ACHALG_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN2586336.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(38375..39664)
                     /locus_tag="ACHALG_000910"
     CDS             complement(38375..39664)
                     /locus_tag="ACHALG_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN2586337.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(39636..41153)
                     /locus_tag="ACHALG_000911"
     CDS             complement(39636..41153)
                     /locus_tag="ACHALG_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HFN2586338.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            41351..42241
                     /locus_tag="ACHALG_000912"
     CDS             41351..42241
                     /locus_tag="ACHALG_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFN2586339.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(42314..43138)
                     /gene="nadE"
                     /locus_tag="ACHALG_000913"
     CDS             complement(42314..43138)
                     /gene="nadE"
                     /locus_tag="ACHALG_000913"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFN2586340.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(43150..44622)
                     /locus_tag="ACHALG_000914"
     CDS             complement(43150..44622)
                     /locus_tag="ACHALG_000914"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFN2586341.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(45258..45620)
                     /locus_tag="ACHALG_000915"
     CDS             complement(45258..45620)
                     /locus_tag="ACHALG_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFN2586342.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            45843..46595
                     /locus_tag="ACHALG_000916"
     CDS             45843..46595
                     /locus_tag="ACHALG_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFN2586343.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(46735..47658)
                     /locus_tag="ACHALG_000917"
     CDS             complement(46735..47658)
                     /locus_tag="ACHALG_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN2586344.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(47673..47945)
                     /locus_tag="ACHALG_000918"
     CDS             complement(47673..47945)
                     /locus_tag="ACHALG_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2586345.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        14008 a         9982 c         8326 g        15800 t
ORIGIN      
        1 aaaaatttcg tcacattccc ctttttcgta tcctatttta agcgacttgc ttggcataac
       61 ggttcacgtt ttgcttgata ttcccgattg ttttcggatc attgattcct gtgacttctt
      121 gaatcaattg ttccaagttt tcatggatct tcatttcatg caattgacgg ctttgttcat
      181 cttcaggatc atagtaatta aaaataatcc ccattgttgc cgtcaaatgt gggcaagtca
      241 agttatgttc ttgtagttct ctcactggac gtgtaaatct ttcttgataa cctaacttac
      301 gtaatggtgt ccgagcgaca cgtgtgatcg catcagagat atttttgttt tggaagcgat
      361 gaatgatttt ttcaatatac gcatgatgct gctctgcatc aaacccccat ttggcaatca
      421 gcaaacttcc tgtttcctgc aaaactgatt ttaattgtgc cacaactaac gcatcttgca
      481 ttgcttcatc aatggtctgg taacctaaca aggcacctgt ataagcgaca gtcgcatgac
      541 cagtattgac actaaatagt ttgcgttcga tatatggttc tagatcatct acatataaaa
      601 ccccttttaa ttggattttt ttattttttc tttggctatc gtcaatgacc cattctttga
      661 aaggttccac ttggacgaat aaaggatctt catggtgttg taatgggacg attcgatcga
      721 ctgctgcatt tggaaagccg atatatttgt cagtaaatga tgtttccgga agatatttat
      781 acacttcttt ttctagaaat gctgaaccgc caatcatatt ttcacaggca atgatatcta
      841 aagatgttgt gcttccttcc ttttctcttt cttggatacc ttttgcaatc aactcagcaa
      901 taaaaggtaa gatattcggg ccaatggcgg tagtaactaa atctgcttca gcgatttctt
      961 ggattacttt ttctggattt gtttgattgt tgattccttt gacatttttt acagtgattt
     1021 tttcttttga ttcatctgct aattcgattt cgtattcttt tctttcattt aaagcatcaa
     1081 tgatcgtgtg attcacatct acgaaagtaa tctcaaatcc attagctgcc aaaacctctc
     1141 caataaatcc tcgaccgata tttcctgcac caaaatgtac tgctctcatt tttctctctc
     1201 cttaagcaat tgctagattt tccgttactt cttctttgct gagcgcgtct gctagctgga
     1261 caacattgtc catatcactg caataaagtg cgatttgctg tacaagttgc aaatgctcat
     1321 ctccgactcc tgcaataccg aacaaaacag tcgcaatctt ttcttcttct tcggtaccaa
     1381 aatctacacc atctggtact tgaacgacac agatgccgga ttttttcaca tatttttttg
     1441 cttcatctgt tccatgagga atcgcgataa aattccccat ataaacagat aacattttgt
     1501 ctctatctac catggcatcg atatattcct catcaacgca ccctgcttct acaagcatct
     1561 gaccgcaata gcggatcgct tcttctttgg ttttaaaaga tcgattgagc tcgatcatgt
     1621 cgaatggcaa actttgcatg ctgttttcct cccttaagct gtctgtttca aatattgtgc
     1681 aatcgattcg tatttttgcg gattgaccat gctgttgact gataaatgaa tggattgagg
     1741 tgcagccatc tgtacttttt ccgctaaact ttctttggct acgatcagtg aatcaggttc
     1801 atcttttaaa tcttggatat tgacttttct taacgggata gttaaatggt tcttgtctaa
     1861 tatatctttg agcacggtca tccccattgt tgctgatccg gaacgatcat cgtggacaag
     1921 tagaatctct ttgatttgtt tgatgtttgc tgaaagaaac tcttctgctg gctcattctc
     1981 aatttcagaa actgatgttt tctgcttgct gcttaattct tgaatgattt catcatatcg
     2041 aggtgaattc aaaaagtttt ctactgagac aaaacgcgcg ctcggtgctt tttgttttgc
     2101 tctttcttgt agttcgacct gagtaacgat caatgtattc gagtcatctg tcagttggct
     2161 gattgcttga ttggtcaccg tttgaggcaa gtctgcttcc tgtacttttt ttctcaagat
     2221 cgaagcgccc atcgcacttg atcccattcc tgcatcacaa gcaaaaatga ttttttgtac
     2281 gccagagaat gtagctcctt gaggaatacc ttgggcacct ttggaagctt gcttagctgc
     2341 ctttgtctct tgaacagatt tttcgaagtc ttcatttgta tctttcgcat ccgctttcaa
     2401 aattactgca gaaataccaa acgaaacagc tgcggcaaca atcacgccca aaataacagg
     2461 aaggtaagaa ttgaacggtg tcatcgctag aatcgcaatg atcgaccctg gagaagctgg
     2521 cgctcttagt ccagcatcca tcaattggaa aatgaaactt cctgacattc ctccgcctat
     2581 aactgataag aataataacg gtttcatcat aacgtaagga aaatagattt catggatccc
     2641 gcctaaaaag tgaatgatca tggctcctgg tgctgaagat tttgctgctc ctttgccaaa
     2701 caaagtaaaa gcaagtaata cgccaagtcc tggaccagga tttgcttcaa gcaagaatag
     2761 aatagatttt ccagcctctg ctacttgttc agttcccaat ggtgtcaaaa tcccatgatt
     2821 gattgcatta ttcaaaaata agatttttgc tggttcaata aaaatacttg tcaatggaat
     2881 caaatgggca gataaaatga tctctacacc ttttgccatt gcttgagtca atgcatcgac
     2941 aactggacca atcgcagaaa aacctaataa cgctaatcca aaaccgataa gacctgctga
     3001 aaaattattg actagcattt caaaaccgga tttgatccgt ttttggaata gctgatcaaa
     3061 tttcttgatg gcatatccac caagaggtcc catgatcatc gcgcctagca tcattggaat
     3121 atctgttccg acaatcacac ccatcgttgc aattgcccca acaactgatc cgcggtcacc
     3181 agcaattact tttccgccag tataaccgat caataaagga atcagataag ttagcattgg
     3241 gcttaccatg gtagcgaaag cctcatttgg taaataacca tctgggataa atagagccgt
     3301 taatacaccc caggcaatca gtgctccaat atttggcatg accatgcttg ataaagtact
     3361 gcctaatttt tgtaccctcg cttttagcga aatgttgttt ttgggaatcg tccgttccat
     3421 tttgtctctc cctctctcct tttgatactc taattgtatc cgcttactaa aaagagaaat
     3481 agtcgtactt tggcacgaaa ctttgtgcca aaggtttgag tatgtcagca aaataaaaag
     3541 atttcggtat cagaaaactt caattttaaa taaaactgtt attttaattt ttttataata
     3601 tgcaaacaat aacttttgtt agcatttaat ttagatgttt tccattctat tatgttatta
     3661 aattttatat tggaggccta tctatgacat tactgatcaa aaacatgaac atgaaacgtg
     3721 gtaaaaaacg tgtgattcga caattgaatc ttttagtaga atcaggagaa gtagctgctt
     3781 tgattgcccc gaacggattt ggcaaaacaa ctttgctgaa tggcattgcc caactgctgc
     3841 caacaactta tgatcaattg tcccttcaca acatctctcc tactgcttat acagattatc
     3901 gccggtcttt cttttttcta gaatcaacga atcagttatc cgactatcta tcggctgttg
     3961 attatttgac tttgatcaaa tactattggc agagcaaagt agaatgccaa actgttttcc
     4021 aacgattgaa gatgactgag taccttactt tgccgattgg aaaaatgtct ctaggaatga
     4081 aacaacatgt cttgcttgct gcttacttga tcagtgattc atctgtcatg ttattcgatg
     4141 aaccgttgaa cggattagac ccaggaagta ttgatatctt gaatagcctc atcagacaat
     4201 ggcagaaaga ggggaagacg attcttcttt cttcccacgc tatcgcgaat atccaatcga
     4261 tttgttctaa agcatgtttt ctaaaaaatg gagaaatcgt cacagaatcc tcaaatatgg
     4321 aagagatttt agctatttat cgtaaattat atgaggtgta actatgtctt tacttacatt
     4381 tgaacgcaat ctcttactaa aaaacaaagt aaacttttta tttcctctat tgttggtcgt
     4441 gctctttgca ttcccacttt tttttgatca taaacttgct tatacagaat tcgatgaact
     4501 gaatcataac tatgaagaga tgcaacgttt gattgaaacg ttgaaagaag atgagaatga
     4561 gaaagaattt gtggaaagtt tagagaaaag caacaaactg atcgaagcaa tcttacatgc
     4621 aaaaaacaca gggaatgtcc agcaaatggt cgaagcaact tatcattatg aaaaagatat
     4681 actggatcga ctgatttctg gtcaaagaca aggaattcca atcattgaac aacaaaaacg
     4741 tgtcgagttg ctccgttata tgaaagaaca tcagatccaa agatattcta tttttgactt
     4801 gccagcgcat ctctctttag caaattacta cgaaaatatt ttttcaggaa tgatttctag
     4861 ttttttgatt ttatgtataa ccgccttgtt tctttcttcc atcatctctt atgaaaaaag
     4921 aaagcaggta atctctctag tcaatctatt accggatagt atggtaaaga aacatagcat
     4981 ccgttttacc atttattatg gtgcagctat gctaagtctc gtgatgccat ttttgatcgt
     5041 ctctatacta gtaataatca aaaatggttt gggagacttc cgttatcctg taggaacgat
     5101 cattggtcag gagatacgaa tcctgccgat gtatgagtat cttttccaga gctttctgtt
     5161 cctgttgttg tgggtgctct ttttaagtac aatcagtttt cttctatctg ctttatttga
     5221 acattctcta gtcaatcttt taggtacact tctttgtctt ttcttagcag agtatcgctt
     5281 gttttcttcg attggatgga tagaatcaat cagtcattac ttgccaactt cttatgttga
     5341 ttttcaaaat gtgatcatag gaggagatat tttctcgcca ctagcttcag aacaggttac
     5401 tttcatgaat ggtatcttga cacttggtat atggagtatc gttttattat tcataggtat
     5461 gggcactatc tatataaaaa agagctatta aaacacaaaa ataatagggg cggtgacaaa
     5521 aaccttgtca ccgtccccta tttcaaataa acggttttct agaataaata gttttcaaaa
     5581 gcttgcatcc gcagtaataa gaacctccca acaaaaatag ctcctcttat ttttatcttg
     5641 gttgaagtta ttacttgatg caaggcagct ttttttactg tgaacattgg ttatatatgt
     5701 ccatgttcaa aaatcatttg gacttcttca atggtttccg catcactgag cgccattact
     5761 tggtcgatgt ctgaacagaa aaaggctaaa tcttggagca ccgctaactg atgttctttt
     5821 tttaaagcca ctacaaataa gagtgtcgcg atttttggtt cctcttttgt tccaaagtcc
     5881 acaccatcag gcacttggac taaatagatt ccttcttcta ctacttcttc atctttgtca
     5941 gtcccatgtg gcaaggctac gaaattcccg atatagacac tcgctttttc ttgtctttcg
     6001 atcattgatt ggatatatgc cggtttgatt cccccttgtt gggtcatcca ctggccgatc
     6061 tcggtcaacg cttctttcca attttgatag gattgatcga gcaatatttt ttctgctggt
     6121 agctgtatca tcttttcacc ttcatttctt tatttcttca atcaggattt tcgctaacaa
     6181 ctgataaacg atttctttat ttcctgagcg gaagatctcg gtgttcaagt cattcatgat
     6241 gattgctcca ctgatctttc ctaaaagaat cccgtctttt tcttggattg gcgaaggcgc
     6301 cagcataact aacatacggg acaaatcgac tggctgtcta tccatccctt caatggtcaa
     6361 cggatgtttc aaattgaaaa tacaaaagat cggtttagaa atcaatgagc tggatgtatg
     6421 gaataacccc atatttgtat gaggaatacc gataggtgct tgttcatatc gcttaagcag
     6481 ccggcgatga accacctctt catccgttac tagtccttca ggaaaataag gaaataagct
     6541 gcgaatcgtt tcggaaatcg tctcaggatt atccagttct tggataaaaa aacgaccgag
     6601 taattgattg atttgatcca tgaaatgcat tgtttcctca taagtatctt ctatcatcca
     6661 ctcgttattg ctgttatacg taattttttg cctttttttc ttctcaatat gcgaaaaagc
     6721 ttcttttagc tgcttgactt catcttctag cagtagcggg gagactaaaa tatattttcc
     6781 accgtatcct ggtaaaatag atgtagaaat aacaacatca tattcttctt gtaaatcgat
     6841 tttttttaaa tcagcaatcc gatagaaatg aatttgttgg atgaagggaa aatacctttt
     6901 caatcgtagt tccaacatgc tagtagaagc taatccactt ggcgaaaatc ctgcaatact
     6961 gatattgctt tctttcggac ttcgttccaa tgaattcgca aaatgaagga ccatataagc
     7021 gatctcttct tcggataatt ttttttcttg aaaaatccgc tctactgctt tacggattgc
     7081 attcgcaact tcctcgtatt gaaccatgat tttttctaaa atcggattgg tcagtgtctc
     7141 ttcttgtaaa acggctcttg aaaacacacc agataaatga gtcaacagca ttttgtacaa
     7201 agtttcatct tcataaaaat ggacattcgt ctcttcactt acttcttgaa tcagttgttt
     7261 tactaaataa gcaagctgtg tatcgaacgt ctcttcaaag aaattctgat caaatgagtt
     7321 cgtgaagtca tttaataatc ctgcaaaaaa gacgatctca tttactggat aaagttgttt
     7381 tgtctcagaa gcgatccgtg aaaacaatcg tttagctgtc tgtagtaatt ctttgtttgc
     7441 cagactatta tatgtctcat ttgtcagtaa atgatttttc gtcacacgct ccatcgtcaa
     7501 tgtcagcatc aaaaccaaat gttcaagctt tctatcactt agattggtct ttttttggat
     7561 caattcttcc ccgattactt tgcgtgcaag agatagatgt tgcggatcaa cgaatcgaaa
     7621 aaaataattc agtgatgcat cgaaagaaat actatgaaaa agttcatatt cattgatttc
     7681 taattctaat acattcgctg tcagaagccg gcggtatttt tccggtcctt cgatctcgta
     7741 tccctggttc cgaatgattt ccaaaggcag ttggcgaata ctttcttcca actgtttgat
     7801 atctgcataa aaagtggtat tactgatgcg atatgattcc atgaaatgag caagactcaa
     7861 cggctctgtc gtcagtaaca actcggctaa aatcgcgtgt tgtcgtttag cagtggataa
     7921 gtcattactt ttcccatctg tattttcttt taattgctct aatacttctt ctgatgcctc
     7981 aatgacaaat gagcctctag ctgctttttt caaagaagca tttaccgatt tcaatgagac
     8041 ttcaagatta tctaattcgc ggtaaatagt ccgtttactg accttcaata aattcatcat
     8101 tttttggata gatacaggtg acgggctact gagaaattct gctaataatt gtttttctct
     8161 gggcgataaa tacatcttat atttctccct tcacgagttt ttcgtattca ttcgtagcta
     8221 cccaatgttc aactagaact agccgaggga tcttttcttc tagctgttca gttacagcta
     8281 gttctctttg tgcgatataa atcgtttctt cttggatttt gattttttct attgattctt
     8341 ttgagataac aaactgtttt ttcgcttttt ttgctaattg acttaaaatc gtcatgccca
     8401 tagtttgtga gccgcgtctg ttgtttttat acaaaaatac aatattagca ggcgacgttt
     8461 tttgttctgt gatatctgct atctcttttt tttcttcccg ctttggaaat tggatcgctt
     8521 cttttattcg tgacagcaag atatctgttt ccaaaaagtg atccagtaca tagatttgac
     8581 tttgaggtac tttttctctt gcaagacccg ctagttcatg ttgcacgata accagctgat
     8641 ttacttggtt attcaattca tagatagagc tgtatccgac agaaagatcg attttttctg
     8701 ctgctaattt ctttttcagc agggcggctc ccatagcact tgatcccata cctgagtcgc
     8761 aagcaaaaat gacggaatga atgatagatg gttccaagat ttctcccctt tctttgaccg
     8821 ttttgttctc tttttcttta tttggtaatt tcaatagtat cgctgcacaa atgaaggtca
     8881 caaatgtact gattgctatc cctatactta ccccagctag ttgtggataa ggcgcattgg
     8941 caaaaatcac gagtagtgac ccaggcgata caggtgcagc taatcccgta tctaaccatt
     9001 gaaatatcaa cgtgccgctt gccccaccag ccattacagc taaaaataga tacggtcgca
     9061 gcaagacaaa tggaaaatag atttcgtgaa tcccgccaat cgcatggatc atcaatgctc
     9121 cactcgcatt tactttttcc tgtttttctg caaaacaaag gatagaaagt aagatcccta
     9181 aaccaggtcc aggatttgcc tccatcaaaa ataaggaaga gtacccgact gtttgtgaga
     9241 attcgattcc taaaggtgtc aatacaccat gattgattgc attattgaaa aatagtactt
     9301 ttagaggttc taagatcaga ctgacaagtg gtaacagatt cattgtgact agccaagaca
     9361 caacggaggc gagcaacaag tgaacactat ttaaaactgg ttcaataaaa acgattccaa
     9421 ttatacaaat aacgctacca gtcagtccta ctagtaggtt gctgacaagc atttcatatc
     9481 cagccttgaa tttcacatgg aatctagaaa caaattgttc atatacgatg ccggcaagcg
     9541 gcccgataat catcgctcct acgatttgtg gagcattgga acgagcgatc aaccctaaag
     9601 aagcaattgc accggcgact gccccttttt gtccccctac cgatttcccg cctgaataac
     9661 tgatcaagat gggtaaaagc agctggatca tccaatggca tacctcttct aatgtggtgt
     9721 gcaaatcgcc agacacacct tttgttgcta atgacaagat tccccaagcg ataaacagac
     9781 ttagatttgg catgatcatt tcatttaagt gatgtccagc ttttctcagt tgtttcatag
     9841 acacgcgact gctgttgttt gctttccatt tgtattccat ctttttcacc agacttctat
     9901 ttattgatgg acggatttta tgtagaaaaa gcagcaaacg aaaaaccgtc tgctgctttc
     9961 agctatctgg gaactatttg cctggttcag acgcaatgat tgctagttca ccttggaccg
    10021 cccactcaga aacgccattt ggaataatac agcttgttcc tttttctaat ggatatgttt
    10081 ttccgtcaac gacaaggtct cctgctcctt cgattaccgt catcaatgta taaggggctt
    10141 gttttttgaa agatgtgatt cctttaatat cccactcata tacattgaaa aactcagttt
    10201 ctaaataagt gacgatcgat gaattccctt ttcggacttc tttgatttgt aattctggtg
    10261 ttttcgcagg gacagttgtt acatcgatcg attgttggat atgcagctct cttgtttgtc
    10321 cttgcgcatc ttttctgtcg tagtcataca cgcggtaagt cgtgtcagaa ctttgctgtg
    10381 tttctaaaat catgattcct ttaccaatag catggatcgt tccgcttggt acataaaaga
    10441 aatcaccttt tttaactggt actttcttca aaaggtcatc ccagcgccca tctttgatca
    10501 tctcagctag ttcttctctt gtctttgcat gatgaccata gatgatttct gcaccaggtt
    10561 cagcatcgat gatataccag cattcagttt ttcctaattc accttcatgt ttcatcccat
    10621 aggcatcatc tggatgtact tgtacagata gatcatcttc tgcgtctaag atcttgatca
    10681 ataatggaaa cactggttct gaaggatggc caaacaattc cttgtgttct gaccatagtt
    10741 catctaatct tttccctttg aattcgccat tttctactac actgacacca tgaggatgcg
    10801 cactgatcgc ccaatcttct ccgattttat cgttagggat atcaaaacca aagactgaac
    10861 gtaaacgact tcctccccaa attttttctt gaaaaactgg ttttaaaaac ataggttcct
    10921 gcataattca aactcctctc atttttcata gctattatag cattatttcg gtaataccgc
    10981 tttctctttc aaaaagtttt caacaatttc atctcgacat atgagatact ccgcacgatg
    11041 atcgacagga tgtacgcgat ttgacaaaaa tatcaacgct tcttgtgttt ttatatcaat
    11101 caagaaaaac gtacctgtat aaccagtatg aaaaagaatc gcatgaccgt cttctacatc
    11161 cttttttaag tcccatccta aagaacgata gccatttttt ttcggtgttt gatcttgcag
    11221 taaagaatca atcgtctctt cttctaaaaa acgaccatta cggatttttc ccttgttcaa
    11281 atacatttgg acaaaagtaa tgctgtcttt caaatcaata aagagacccg cgttacctgc
    11341 atgttcagcc aaaactcttg ctttcggatc atgagtttgt ccacgcaaaa cctcacccga
    11401 aggcagttgt tctgtgggaa cgattttttt ggaaggaaaa ggaagaaata agctgttcat
    11461 cattcctaat ggttccaata cttgttcttg aaagatttcc actgcagttt cttggtaaat
    11521 ttcctctagc atcaacccaa gaagaatcgt accggcatct gtatatttta cttcttttcc
    11581 tagttgctca cctggttcta gcttcaaata agcggcgatc aattcttcct gagatagctt
    11641 atctctgttt ggtatccatg ttttgatatc agaagtatgt gtcagtaaat gacgcaaagt
    11701 gattgtctga ttttcaaatt caggaagata tttatgaagc ggttgatcga gttctatctt
    11761 tccatcttct aatagacgta aaactaatga agtggttgca acgactttcg tcagtgaggc
    11821 tgcatcaaac aacagatctt ctgtcatcag ctgcttttca ggaacgactt gggcaaagcc
    11881 ggctgtaaat gtttctactt gattttcttt tatgaaggcg actgctacac ctggataaaa
    11941 tccttcttcc atctttttca aaataagctg tttcgtgtgt ggatacatct ttttctcctg
    12001 cttttctatc agattcttac agtccaagct taaagcttaa tctatgagga atcaaccttt
    12061 tttacacaaa aaaactgcgc gaaaaaccgc gcagtttccc ccggtatact attttctctt
    12121 tctatctcta gaataagcga tagtatgacc gttcataaaa tgacgagaca tgcgacaatc
    12181 gttaagaaag aaaaagttaa gcgtcgcatc gaatgtcagt tttattcttt tcttttggct
    12241 cactgacgaa ccaccattcg attcctatag catcatagat tgtgagtact gtgtgctttt
    12301 tgtctaagaa gaaatcaact gatagttgct gcaagtgagc aatcagtcct tccatgcttt
    12361 cagtatcgat catgaatttc agaaaatcaa gtccaatcac ttcatctgta ggcaaatcaa
    12421 ttgcgcctcc gctagcttct gtcaaaccaa catggaaatc tccttcattc aatacatgga
    12481 tgccctcttc ttcattatgt accttcaaac caagtacatg attaagaaat tggcgttctt
    12541 ttgttaagtt agtaacattt aaatggattt tttcaaaata agaacctgtt gatagtttac
    12601 tgaatttttc ttcagatttt cttaataatg cttcttgatc cattgtttct ggaatttctc
    12661 tttggtcgtt cttattctct tcatggtaaa tttccaattc attctcttct gggtctacga
    12721 gcaaaatccc catacgtcca tgatcttcta aagcttcttt gattggataa ttcattttac
    12781 ggatacgaca aaggatatct gccatttctt ctgtgctagg gataatcaat gaaaaacggt
    12841 tcagcttttt gatcaccccc atgtgatcat tcgctctagg actttcttcc agcagcaaca
    12901 tttcacttcc agcttcttct gttcctaaaa tggctaattc gttttcttct cttttcaaat
    12961 caaagccaac aacgttcttg taaaaatcaa tcattttatc tcggtctttc acccgaatgg
    13021 ctacactttt taaataggtg gcagccccca attgaaaatc tttcatattt cttgctcccc
    13081 tctacgtcaa gctttatttt aacgatggat tcttcttctt tcaagcaata actttgattt
    13141 tgttaaaaaa aatgtgagaa aattcgcatt ttgcttgttt tttgtaaatt aatgagtaaa
    13201 aactcttata aaaagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
    13261 acaaaaatag ctcctcctat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt
    13321 tgaacaccgt ttaaggaaga ggttgtgaaa aagtcgctta gtttcgagta gtaagaaaaa
    13381 ttttgtgaaa atggctttct acatttttgc ataaatttgg cttaataccg agaaactggc
    13441 ttttgaacac cgtttaccaa gaggttgtga gcctgccgtt taactccgag tcacaaggag
    13501 caatttcaaa aaacagcttc tcaggtttgt tgactattgt gacttgtgac cgaggagttg
    13561 gcaggagaac accgtttaag gaagaggttg taaaatcagc ggtttgacct gagtattaga
    13621 gatacaaaac ggaaaaatag cttctcatat tttgccgttt tttgagctaa taccgaagga
    13681 cagctgattg aacaccgttt attcggaaaa agggactgtg acacgacttt tgtcacagcc
    13741 cctaaaaaaa ataataaatt tgtactattc tatgaagctt cttcttcaga aaactgactg
    13801 ttataaagat ctgcatagaa tccgtctttt tccatcaatg tatcgtggtt tcctgtttct
    13861 acgatcgaac catgattcat cacaataatg ttgtctgcat cgcgtattgt agacaagcgg
    13921 tgagcaacaa cgaaacttgt ccggttttcc aataggcggt tcatcgcttt ttgaatcagt
    13981 atttctgttc gtgtatctac actagatgtt gcttcatcaa ggatcaggac atctggattt
    14041 gctaagaatg cccgcgcaat tgtgattagc tgacgctgcc cttgcgaaat attgcttgca
    14101 tcctcgttta atacagtgtt atatccttct ggcaatttac gaacaaaatc gtctacgtga
    14161 gcagctttag cagcctcaat gatttgttcg tctgttgcat cgtcgttccc atatttgata
    14221 ttgtcataga tacttcctgt aaacagccaa gtatcttgca gtaccattga aaactgtgcg
    14281 cgcaattcat cacgtgaaag atcacgggta tctacaccat cgtaacgaat actacctcca
    14341 gaaacatcgt agaaccgttc aagaaggttg atcagtgtcg tcttccctgc accagtcgga
    14401 cctacaatag cgaccatctc accaggtttg acatccaagt taaaatcagt cattagcaag
    14461 ttatctggtg tgtagccgaa ttgaacattt tcaaaacgaa ctttgtatgg tgtatctttt
    14521 tcttctggaa ggccagattt ttcattggac atctcttctt catccaacac ttcaaatacc
    14581 cgttcggctg aagcaactgt tgattggata gtgttcatca aacttgcaag ttgagtgatt
    14641 ggttgagaga actgtgttgt atattgcaag aacgcttgaa catcccctaa tgtcatgttc
    14701 ccatttgcaa cttttactcc gcctagtaca gcgacgaaca catagcctag atttttaacg
    14761 aaattcatca acggcatgat gattgccgaa ataaattgag ctttccaacc agcggcatac
    14821 agattctgat tttctttttc aaatacttcc tggtcttttt cagtatgatt gaatgtttta
    14881 acaatggtat gaccaccata tgtttcttct acttggttat tcaataaacc taaacttttt
    14941 tgctgatctg cgaaaaacac ttgtgatttc ggcgctacga tcatcacgat gatcaagctt
    15001 aaagggatca tcaataacgc gactaacgtc aattgccagc tgattgaaag catcatcacg
    15061 atgaccccaa tcaatgtgac tgcactagtt acaaattgag tcaaattttg ctgcagcgtg
    15121 cttgcgatgt tatccatatc attgattgct cgagacatga tatccccatt tgtatgtgta
    15181 tcataatagg aaatcggtac acgattcatt ttggcttcca aatcatgacg taattcataa
    15241 actgttcgtt gggaaacacg tgtcataata aattgctgta aaaagttgaa tacagcagaa
    15301 atcaaataca tcacgattac tgtcgcaatg atctgcgcga ttttatcaaa gtctatcgga
    15361 aattttgtta cttgttggcc ttgttgcatc tgttgagcgc cagccattac acctttgaat
    15421 atttctgtgg ttgctttccc caagaccttt ggtgtttgga tttggaagat cacggctgct
    15481 attgctagta caaatacagc gatgatcgca accatacgtt tagacatata gccaaacaat
    15541 cggcgtaccg ttccccagaa atttttaggc ttttcacctt ttacaccgag atttctgcca
    15601 ggtccaccat gtcctggacc gcctgctggt ccatgagagg gacgtgtgtt tgttttactc
    15661 atgctaagtc ctcctctctt agttgtgaat gaacgatttc ttgataagtt tcgtttgttg
    15721 ctaatagttc ttcatgtgtt cctttgccaa ccagttttcc ttcatctagc acaaggatca
    15781 tatctgcatc cattactgta cttactcgtt gagcaactaa aacggtgata ctttctttca
    15841 tattcgtttt caatgcttgg cgaagattgg catccgtttt gaaatccaac gctgagaagg
    15901 aatcatcaaa cacatagata tctgctgttt tgactaatgc ccgtgcaatt gccagacgct
    15961 gtctttgtcc accagagaag tttcctcctc cttgttccac atggctatct aagccatctt
    16021 ccatctctga aacaaaatct ttagcttgtg cgatttctaa tgctttccaa atcatttcat
    16081 ctgttgcatc cggcttacca tactgcatat tgtctcggat cgttccagag aacagaactg
    16141 ctttttgagg aacaaagccc attaattctc gcaaattata ttgagtcatg tcactaatgt
    16201 ccgttccatt tatttggatc gaaccagatt cgatgtcata taatcgaggg atcaaattga
    16261 ctaaggtcgt tttacctgaa ccagtccccc ctatgattgc gacgatctcg cccgacttgg
    16321 cttggaaatc aatatcttcc aatgcaagtt tttctgctcc atgatatcga taattgacat
    16381 gattgaaggc caatgttgcg ttttttccag caaaagaaac tgttttagga ttttcagggt
    16441 catggatacc gattttttca tccaacactt cattaatacg gtcagctgaa gcttgtgcac
    16501 gaggcaccat gataaaaata gcggaaagca tcataaagct gatcaagatc tgcatcgcat
    16561 aagtcataaa tgcaatcaga ttaccgactt ccaaagtcat atctgctatg tagtgtccac
    16621 caaaccaagt gattgctacg tttgtaccgc tcatgatcaa tgtcatcatt ggcatcatca
    16681 atgcgacgat cgtattaact ttgattgccg tatttgtata atctttattc gctaaatcaa
    16741 aacgattttc ttcaaagcgt gtcttatcga atgctcgaat cactctgacc cctgtcaatc
    16801 cttctcgaaa taccaggttt aaacggtcag ttttcttttg catacttttg aataatggaa
    16861 cagcaaagta catgattccg ccgatcaaaa taatcattac tgggatgaca actaagaaga
    16921 tttttgtcaa ctgatgatct ttttgataag caagtatgct cgcgccaatc aacgtgattg
    16981 gtgcattgat catcatacgt aaaaacattt gtgtaaccat ttggatttga gtcacatcat
    17041 tggtcgttct tgtgatcaaa gaggccgttc catatttatc aaacgcatct tttgtcagac
    17101 tctctgattt tttatagaca tccgtacgca attgcttacc cagtttctgt gattcacgag
    17161 tagcaaaaaa tgtatttgcg atagcagcta agatactgat caaagaaaag ccgatcatca
    17221 caaaacctgt atgccaaata taatcgacat ctccttgcgc taccccttta tcaataatat
    17281 tggaggtaag tgtcggcaaa tataaatctg ccagtacttg gatcaccatg aaaactgcag
    17341 ctgcaattgc tgaagtcaag gagatccgtt ttacgatttt tagcatctag ttcccttctt
    17401 tctatttata tactccgttt ttcagccaag aaagtttcaa attgaattca ctgattgctt
    17461 gctctactga gtaatcttca gatgttgaaa ataagcgata cgaatgcgca attgttgtaa
    17521 atacagtatt catcactaat tggatcagta attccaattc atgatcattt tttactttca
    17581 gcttcgtccg atcgatcaat tcaagcaact cttttgcatc ttgtttgtgt tctttatgtg
    17641 gatcgtgagg cgttttttgg cgactcgtat gaggagcgac ttttcttgag gcacggtaat
    17701 ccatgttcat aaacaagtta cgataaaaag aagcattctc gccttccaaa atctcataaa
    17761 tcattttaga aaaataggat tcgaatcctt tgaatagatc accatcggat tctttcatta
    17821 gttgtaacat atctttagag ctgtcacgtc tcattgtctc aaagtaataa aaataaagat
    17881 cctctttatc ttcaaaatac tgatagaaac tgcctctagg gatatccgct aatttgacga
    17941 tattagcaat cgaagcttct tttaagggta ctctagaaaa ctcgattctc gcagcctcga
    18001 ttagtctttt ttgtttttct ttaggcaaat ggaaaaatgt ctgtgtcggc atatggattc
    18061 tcctttcttt caagatgaca cattgtcata tgacgctatg tcattataac acctcctacc
    18121 tgttttgcaa gacatttgca tagttttttt cattatcatc cgatacatta aaaaagtgat
    18181 aagaggaatc cttccatttg aattaatgag agttgcccat taaaatagcc acatactaaa
    18241 caaaggatga gataaatggc ccaatcaatt agtgtccaag acttcaataa attgcctctc
    18301 gatgctgaca gcatcgtttt agatacaaga aataccttag attacacaaa aaaccactta
    18361 agccactcgc tttctatccc cgctgctatc ttgccaagac aattaaaaaa tctagatcca
    18421 aagaaacttt attatattct aagctataca ggcaggcgat cagaagcatt agccgatcaa
    18481 cttactgcta atggttttcg tgctgtttat atcattggtg ggatggaagc tcttcagtcg
    18541 agtgttgctt gaatattgct tgttagattc aaaagaggct gtgacaaaaa atcgtgtcac
    18601 agcctctttt ataccctgtc tttttttagt tatttattct tcaaccttat caatatgact
    18661 tgatagcctt ttctttttcg agtaaactga ttttgttagt catagttata acaatcatag
    18721 caataaaagg agaaggtaag catatggcat ctgttttagt agtcaaaggt catcccctaa
    18781 ctgcagaaga atcgcgcaca gttaaggcat tgacgtcttt tttaacaagt tataaagaaa
    18841 atcatccaga tgatgaagtg actgttttgg aactttatcg cgatgatatt ccggaaatcg
    18901 atgaagaatt attatcaggt tgggaagcat tacgtgctgg tgcagaattc acttccttat
    18961 cagaaagtca acaacaaaaa attgctcgtt tcaatgaatt gactgatcag ttccttgctg
    19021 ctgataaaat cgttatcgca aacgccttat ggaatctaaa tatcccaaca aaattaaaag
    19081 cttggttcga taccgtcaat gtagctggaa aaacgttccg ctatacagaa aacgggccag
    19141 aaggattagt gacaggtaaa aaagcactgc acatccaatc taatggcggt gtatacaacg
    19201 gacaagactt tgcctctcaa tatgtaaaag gtattttaaa ctttgtaggc atcgatcaag
    19261 tagatcagct attcatcgaa ggaatcgatt atgaccctga ccgtgcagat gagttgatgc
    19321 aaaatgcatt agataaagct gctgctttag gtaaatcatt ttaaaaattg agataaatgc
    19381 atacaaaaga gacagaggaa aaaatcttca aagcagcatt tgtgccttgg atattttccc
    19441 atctgtctct ttttgttatt ctttctgctt tttcaacgga ttttcttcat aaagagaaga
    19501 gattgttgtg taccattcaa aaacatgagt aatcactact ttagctgctg cataaacagg
    19561 aatccccaag atcacaccga tgattccgaa caatttccca gaagtcagca gaactaataa
    19621 aatcgttact ggatggattg ctaactgatt ccctagtact aatggagaga tcaagcgtcc
    19681 ttctattgtc tgctctactg caaaaacgat cagtactttg atgatcatga atggacctgc
    19741 tacaatggct aaaaagatag ccggaatcat cgctaaaaat gatcctaagt aagggatcag
    19801 gtttaaaaaa cccgcaataa tccccaacgt gacagcatac tctaaaccaa tgaccgcaaa
    19861 accaatcata aacatgattg ccacagcaaa agccacagtt agttgtcctc ttatataaga
    19921 agaaacttga ctattcattt cactcagcac attcaacgtc ggtttttgca tgcgtgtcgg
    19981 taaaaatttg acaaaataag gtgctaattg tttaccgtct ttcaatagat aaaataagat
    20041 aaacggcatc gtcagcagag ccactaaaat agtcgctacc gcgccgacaa aacttccaag
    20101 actctgaact gtactcttag atatatcttg gatcatatcg cctgccgaat tcatgaactt
    20161 atcacctgct gtttccaact gttcgcgaaa ttggttgaac agcgggtccc gcaaaatctc
    20221 ggttaattta ttatcaatcg tatctacata ttgagggaag ttactgatga agctcactgt
    20281 ttgttcctga atcttaggga tgatcactac gctcccccat gcgattagtg caacaaccaa
    20341 aacaaacagc ccaatgatac tgtaaagcct tggtactttt cgcttttcaa agtaatcaac
    20401 taccggattc atcaagtaat acaagattcc tgctaaaatg ataggcagac ctacgattgc
    20461 taaaaattgc cagattggtg caaacaaata tgaaactttt gtaaataaaa agacgatcag
    20521 caatattaac aatacaacaa gtaaggctgt gactacttga ttatttaaaa accagcgcca
    20581 aaaccacgat accggtctgt tcttcttatt ttttccttcc ataattcctc ctgttagtta
    20641 taaaaaattt ggcttcccaa accaatagca ggcagtctat gcggatgaag atagattcca
    20701 tttgcaattt gtccttcttc tggacaagca tcaaaaatca atgatacgtg agctactgtc
    20761 tttaaattcc cattggccat cggaccgaca taatcgatcg tatatacctg atcatcaaaa
    20821 gcaattgttc cgcctgcttt caaagaaaaa ggttcgtctt ttgatacttc ttgaatcacc
    20881 gaatattttc gtaaggcagc tgttgctgtt ttatcgaaca aaatcagcat gggttcttcc
    20941 ttgtttattg cttctgtccc aatttctgta atctttgcct gaatcattgc actctctcct
    21001 ttaattgctt tcaaaaacag tataacacat tctcattctt tagccgtgtg gaataattat
    21061 gtgatagaat aaaaacgaaa actatttcat tacaattagg aggaatcagc tcatatgatc
    21121 gaaaagataa ttttaggaac ctatacgcgt cgcgaaagta aaggaatcta tacgatcgac
    21181 cttgatacag aaaaagaaga attagccaac ctttcattag ctgttgaaga aaatagccct
    21241 acctatgtag caaaaagtaa agctggcaat ctttacacag ttactagtgt ggatggatta
    21301 ggtggtgccg gtgcgtacga taaggatttt cattttttga atgcggtaac agagtctggc
    21361 gctcctttgt gttatgtagc ggttgatgaa gcacgtcaat tactatacgg ggcgaactat
    21421 cataaaggtg aagtaaatgt ctaccatata ttaggagatg gcgaactgaa agcagcagat
    21481 tcaatattcc atcaagaagc gacaggtcct cataaaaacc aagatcatgc gcatgtccac
    21541 tatacggatc tgactcctga ccaacgatta gtcgtatgtg atcttggtac agatcgtgtg
    21601 tatacttatg atgtgtccga aaacggcaaa ttgaatcttg taactacctt cactgcagag
    21661 ccaggaacag gccctcgcca tcttgtcttt catccaaatg gacaattcgc ttatttattt
    21721 ggtgaattag acagcacagt ccgcgtgctt tcttataata aagaaacagg tgcttttgaa
    21781 gaattacaga aaatatcgac cttgccagaa gaattcgacg gtgaaaatgg aggggcagct
    21841 atccgcattt caaatgatgg caagttcctt tatgcttcta accgaggaca taactcgatt
    21901 gctgtattcg cagtttttga agatggttcc cacattcaaa acaagcaaat catctcaaca
    21961 gaaggggatt ttccccgcga tttcgcacta gatccaagca ataattttgt cgtgtgtgcc
    22021 aatcagaaca ccgataatct gacattatat cgccgtcatc ctgaaacagg attactggaa
    22081 ttactgaaaa aagatgtcta tgtaccagaa tgtgtctgtg tttattttga tcgttagtaa
    22141 gaaaaggaga atgaaatgaa tcagcaagac gtattagaca gactacggga agaactaaat
    22201 attcctttct ttgatgggaa aatcgaagat aaagagtata gcgaagagga atatcagaaa
    22261 ctcaaagccg atttacagaa ctactttgaa caatatgtac agaatgtaga aaactaatca
    22321 gctttccata ctatctgaaa agaacatcag gacaagactt ttccgtctat cctgatgttc
    22381 ttttctacaa ataaaaaacc gccgagatgg ttagactcga cggaaaagga gtatttactc
    22441 gattttgagt aagtacaagg ttgttgttgg ttataggtat gtcattatca taaaggattt
    22501 tgatcactct atcataaagg taatattaca tttatattag ataactctag tttttattgt
    22561 tatcaaagat ttcttttatt ttttcaacaa caaattagtc attttttgga tggcagctgt
    22621 caattggtct gtcgcttggt cttgttttgc ttcagatgta ttgaatagat cttcatctac
    22681 ccctgattca agatcgatac tttggatcat ttcttcacag cctgctacat atgaaagata
    22741 agctttttca aaatttttat gaatgcccat tgcttttgct ggtggacgca aagtactgac
    22801 tttcataagc attgcccgat atttttttgt tccttcttca aataacagga tgatctcttc
    22861 aagagtcgat ttgtccagtt cacttgtttt tccttcatca attgcgtttc tgatcacttc
    22921 atatttcgga tgcattcctt ctccgattgc ttctgtttcc ttgacgattt gatcgatgga
    22981 ctgcacataa tagccaatat tcggtaacat gttcgttttc ctactttctc cattgttgtt
    23041 attatttatt ctacttgttt ttatcctcaa atgacaagta cttgaaaaaa tttaatggtt
    23101 tctatacagc tcgccattct tattcgtgct caaagaaacc atctgctaaa ctatattgtt
    23161 tccctgcgac aatgtcatat tggatcaatt gatattcgtc gtaaatctct tgttgcgagc
    23221 gattgaattg cgcttcttta taccactgtc cgttttgata gtacagttct cgttcaaacg
    23281 ccggaagttc ctgttctaat gataaaagca gctgataaaa gccatttgtt gtctgattcg
    23341 tctgttccat tagattcgct gcaaaataaa acggactggt gattgcatcg tgagtcgttt
    23401 gcttttctaa ttctccttta ctgtcgaaca taagaaattg tgtttcatgc aatgtttgtt
    23461 tgtcattttt tgcttggatc gtatctgaat agatccctgg aagatgatcc ccccaaaaaa
    23521 caactaaagt ccttctagaa agattcttca attcttccgt gaactgcttc agtgccgtgc
    23581 tgctatagga aatatcttgc agataattct ctaagtcaag tgtcccactt ccatctgaag
    23641 tttttgcagg atagtctaga ttatcatact ttccatcgta gggcatatgt gtctgcatcg
    23701 tgactaaatg aatgaactga ggtgtttttt cttccttgag aagtgtcatt acttctttgt
    23761 aagctgactc atcagaaata tatggattat tctctatcgt atccgtatag gtcatcgtac
    23821 gctcgctgat gaactggtca aatcctaatg tctgataaac atcctctctt ttatacatcg
    23881 aagtattgta tggatggatc gctgtcgtct ggtatgattg tgcgtttaac gctgaaacga
    23941 tagaaggaag ctgatccatt tttggtacaa gcatcgtgta aggggtggtg agttgcgcat
    24001 taaataacgc catagaaaaa ccagtcaatg cttcaaattc gatatttgct gttccaccgc
    24061 catagttttg agaaagcata ttgccactat atgtttgatc agctacttca taataatctg
    24121 ccaatggctc accagatact tctacgccgt ttaatcgact aggatctgag aaactttcac
    24181 tcatcacgaa cacgatgttc ggcgactctt cctctacagt tttatttttt tcatcggcta
    24241 gcttctggta tttttcagtg atttctttga tcttttcttt cgaatatcct tcaggttcat
    24301 ccattggttc taccttcaga ttgtacaaaa acccaccgat aaaaccagtg ttatagtagt
    24361 tcattttctg gctgtaaggt atccatagtg ccgttttatt atatgctttt cgcaacaaat
    24421 tatcgggatt gttgaaatta ctgatatacc ctagcagtcc aattgccaca agcagtgtcg
    24481 ttaatcggat caattggaat cgccttcctt ttttcaggct tcgaaacatg taccagcaaa
    24541 acaatcctat cacggttcct gcggcagcta agatcactgt aaacagcata gtgccggtca
    24601 tatctttcag caatccgatt tcagtgatca tcttcaaatc gtctggataa atcggttctt
    24661 gccgataact cattttcata taatttgcaa aacctaatac accgatggct acgacatacg
    24721 taagggaacc taaagggaca gatcctgcta gtgcaatcag aaaaataaga ataatcagca
    24781 agacgagaca ggctaagaag aatttttccg tatgccaaga aaaagcaaat ttcaacgcaa
    24841 ggtctacgga tagatcattt tgacaccact gcaaaaataa attactaaaa acaacaagaa
    24901 aaaagatact gatgattgct gctgttcctt ttaaaatctt tttctgtttg gtcgaaagtc
    24961 tgcttatata tcttttcaaa tgcttgctcc ttcatttcta tcaataaaag aatgttgctt
    25021 ctcttatttt agttgtacga aattcgaaag taaagaacgg aaagaagaat taatgaaatc
    25081 caaatattct ctttctttat ggcctttctg gtttctttaa taagcggacc tttcgtactc
    25141 cagaaatttt ttctagctgt tgtttcactt gattgagctg ttcaaaatca gtactgtcta
    25201 tgtcaatcaa tatgtagaca tgctcattct tatggttacg cgatatatct gcgatcgcta
    25261 gctcgttctc attcagcaga gaaaatatct ctgctaatat attggttttc ttttgataaa
    25321 atatagtgaa tcgaaacggc gcttggaaaa acattcgtgc ggatggataa ttgaccgctt
    25381 ctctaaccgt accgaacaaa agataatctt ttaaggcttc aactgccaaa cgtccgctgt
    25441 ctgccaaagc ttcctgagtc gatcccctag atgaggcaac ataagtatac ggtcattttc
    25501 taagaactct tcttctggaa aatcagtcaa gtatttagcc agatgttgtt tttccaatgc
    25561 ttgcataaca gcatatttat caacaatttc acttcttcct acattgataa gtacagcatc
    25621 ttttttcatt ttttctatat tcgcctgatc gatcaatcct tttgtatctt ctgtcaacgg
    25681 cagcatgact acgatgtaat ccgaggtaga aagaactgtt tctaaatctg cttgccgaac
    25741 ataatccaac tgggcatcgc ggatagaata acccaagaca tccatcccta agctatagca
    25801 acttagcgct acttttgtgc ctatcgctcc aagaccaaga agaccgatgg ttttgccttg
    25861 caattctcgt cccacataag cacttctttt gttttctgcc tgttctaaaa tattaggacc
    25921 tgttagtgtt tgtaccatac gacttgcttc gattatcgga cgggaagata agagcaaaca
    25981 gcacaaaaca agctctttta ctgcattagc atttacacct ggtgtattca tgacgatcgt
    26041 cccgttttca gaagcttttt caacgttgat cgtgttcacc ccaacaccag ctcttgaaat
    26101 tgctagcaaa tctggtgtaa gccattcatc tttcactttc gtactacgaa taactattcc
    26161 ttcggctggc aaatcatcta ttattttgaa atattcccct tgtaactttt ctatctcttg
    26221 ttctggaata gcatcaatca aatgaacgtg atacataaaa ccccctcctt actaacaagt
    26281 atctcgaaat ttctgcaatt agacaactgt ttaccatttg acttcctaga agcaaaaaga
    26341 gcagttagag cagagtaggc tgagtgataa aaataaggct atgacaagtt actgccataa
    26401 gccttatttt cgagagcaaa atattgaggg tgagatatct tgataaatgg tgttccgtta
    26461 gctgacattg ataataagac taaatttcaa aaaagttgaa aagcaaattt cgtcaatctc
    26521 ttcttatttc ttaaaactaa gtggcttttt cacaacctct ttttatctaa taaccactgt
    26581 ttccaactca actaattttg cccacgtttc aatttgatct gttgttagat ttagcgagac
    26641 aacggtatga tgaccgccac ctgcctgaat ccatgaatgg acaccttcat ggaaattcgg
    26701 tttagttttc cataacacac gtgcaacagg taaattaggt gcttcagtcg ttggttcaaa
    26761 ggcttctact tcattgatca gcagtttata atgcgtaccg aaatctgcca tcgatacgac
    26821 taccccttct cctgctttcc catcaaatac aagacgcgcc ggatcttctc tatctcccat
    26881 tgataaagga gaaacaacga ttcttggttt cgtcgcagca agcgtcggat ctacttccat
    26941 catatgggat tccaagattg cttcttggcc actagctagc tcgtaagtat aatcttccat
    27001 gaatcctgta tctttccctc gagccatgat tttcagcaac cgatcaatcg ctgcagtttt
    27061 ccagtcgcct tccccagcaa aaccgtatcc ttctgccatc aaacgttgta cagctagtcc
    27121 aggtagttgt ttcattccat acaggtcttc aaaattagtc gtaaaagcgt tatagcctcc
    27181 ttcttctaag aaacggcgaa taccgatttc ctgctgagct tgtactttta catgttcctc
    27241 ccatgtcttt tcttcataat caccatagtc gaaggtatac aattccttgt actcttcaaa
    27301 taattgatcg acttcttcgt ctgatacgcg atcgattact tgtacaagat ctccgatacc
    27361 aaaataatca acagtccaac caaattgaat ctgtgcttcc actttgtctc cttctgtgac
    27421 acccacgttt ctcatgttgt caccaaaacg agccactttt attccaaaac tttcattata
    27481 ggctactgca acatccatcc aatcagcaat ctctttttgg atctcaggtc gtttccagta
    27541 gccaacgacg attttatttt gtttatttaa tcgagcattg atgaacccat attcgcggtc
    27601 accatgagcg gattgattta aattcatgaa atccatatcg atcgttttcc aaggaatgct
    27661 ttcgttatat tgcgtagcta aatgaagcaa tggtttttgt agaagctttg tcccgcggat
    27721 ccacattttt gctggtgaga aagtatgcat ccaagtgatc acacctgcta cttcttctcg
    27781 ataattgacc tctttcatga tttttgtgat cgtatcagca gtaactgcta attcttgaag
    27841 gcggattgga taaggcaatt ggccattttc attcagttca tcgaccattt cttgtgcatg
    27901 tttctttact tctcgcagtg cttcttcccc atataaatgc tgtgatccta caacaaacca
    27961 aaattctttt tctccaatat tcaacataat tagccactcc ttttatttgt tttgtccgta
    28021 atacgcattt tttccgtgtt ttcgtaaata atgtttatct aaaatccgtt gaggcagctc
    28081 ttctgaaaat gaattgagct gtctggtaaa taaattcatc ttgcatactt catctagtac
    28141 gactgcgttc attaccgcac tttgcgcatc tttcccccaa gtaaatggac cgtgtccgtg
    28201 taaaagtacc cctggaattg ctaagggatc aattttgcgt tctttgaatg tttctatgat
    28261 aacttttcct gtttcatatt cgtacccgct gtcgatttcc tgttgtgtca aaaatcgagc
    28321 acaaggtacc gatccataaa aagtatctgc atgcgtcgtc cccatcgcag gaacatccaa
    28381 tcctgcttgt gcccagatcg tcgcccatgt cgagtgagta tggacgattc ctccgatttg
    28441 aggaaatgaa cgatataaaa cagcatgagt aggcgtatcc gaagagggat tgagtttacc
    28501 ttcaatgata tgattgtcca agtccacaac gaccatgtcg tctgctgtca tctcttcata
    28561 actgactccg ctaggtttga taacgaaata tcgtgtttca ggatcaaaag cgctcacatt
    28621 tccccatgta tatttgacga gtccgtgttt aggcaagtca agatttgctt ggtatacttc
    28681 ttctttcagt tgttccagca ttttctatca ctcctttttc ttgcaattga tgaatcgctg
    28741 ttttttcaat gtcgatcccc tcacggtaac gtctcataaa tatctgatag cctgctattt
    28801 cttctgctga aggtgtgatg gtgattcctt ggtcttgtcc aaacacgtct tctgccaaga
    28861 agttttctaa agacttccca tgtcgatcca tcatataggc tgctaataac gcgattcccc
    28921 acgctccacc ttctccggct gtgtccatca ctgtaactgg tgcctccata gcagatgcta
    28981 gaatccgctg tccaacttct ggtgttttaa aaatcccccc atgccccact aaacgatcaa
    29041 tttggacatt ctcagatttc aatatatcca ttccgattct cattgcacca aaagcactgg
    29101 ctaaatggat ccgcatgaaa ttcgctaaat caaaacggct acctggtgta cgtacaaata
    29161 atggccttcc ttcatttact cctgtaatgt tttcaccgga aaaataacca taagaaagta
    29221 atcccccgca atccgtatcg ccttctagcg ctttgttgaa taataccgtg aataatttat
    29281 ctctgcttat ctccataccc aaagaattgg taaattcttc aaaaatactg acccaagcat
    29341 tgatatccga agagcagtta ttcgtatgaa ccatgcctac tagttctcct gaaggtgtag
    29401 tgaccatgtc gatttccgga tggacatttt ctagttcttt ttctaatacg atcatagcga
    29461 aggcggaagt accagccgaa atgttccctg tccctttggc tacactgttc gtagcgacca
    29521 taccggttcc tgcatcccct tctggcggac aaaccgggat tcctggtctc aaatttcccg
    29581 tagggtcgat cagataagcc cctgattcgc ttaacttgcc agcttcttct cctgctacta
    29641 gaactttagg caaaatgtga tccaagtcaa tcgctacgtg cgctttttcg attaactgac
    29701 caaaaatatt tttcatttct tgatggtagg ttttcgttga aggatcgata ggaaacatgc
    29761 ctgatgcatc tcctacgcct agtactttct caccagtaag ctgccaatgg acataacctg
    29821 caagcgttgt caaaaaatta atttctttca catgttcttc tttattcaaa atagattgat
    29881 acaaatgagc gatactccat ctctgcggaa tattgtattg aaatttttcc gttaatattc
    29941 gttctgcttc ttcagtgatt gtatttcgcc aagtacgaaa tggtactagt aactgttcct
    30001 tgctatcaaa tgctagataa ccgtgcatca ttgcactgaa cccaatcgac ccgattgtcg
    30061 taagattctc gccatagatt tcgaatactt ctgctgccat ttttcggtaa ctgacgcgca
    30121 gtcctttcca aatatcttct aatgaatagg tccagatacc attttctagt ttgttttccc
    30181 actcataact accagcggca ataggagtaa aagatgaatc aatcaataca gctttgatcc
    30241 tagttgatcc aaactcaatc cctaaagatg tacgaccatc tatgacatct aacatacttt
    30301 gcgcatttac ttccttcata atgatttaac tccctccttc tcgtcaaacc tgatcaaacc
    30361 gtctttttag tagcgcttac aatgattatt gtatactttt gtacgtacat gtcaagcagt
    30421 tatttacaag aatacaataa aaaacacttg ttttgtacgt acataagtta taaaatgaaa
    30481 ttgcttacaa aatctgatca accgccttct cgaaattcta gaaggagaga atacttatga
    30541 aaaaagaaaa aaaagtttcc tcatcgttta tttatttttt tggcgcattt ggcggcattc
    30601 tatttggata tgatattggt gttatgaccg gcgccttacc ttttttacag catgattggg
    30661 gtttggcagg taaagcttct ttgataggat ggatcacgtc ttcggtcatg ttaggtgcga
    30721 tccttggcgg ctctttatct ggtcttcttt ctgataagct tggacgacga aaaatgattc
    30781 ttctttctgc acttattttt atggctggtt ctgtcctttc tgcatctgcc cctcacaatg
    30841 gttcttattt tctgattgca gcaaggattc ttctaggttt agctgttggc gctgcatctg
    30901 ccctagttcc tgcttatatg tctgaaatgg ctcctgctag actaagggga agattgtcag
    30961 ggatcaatca agtgatgatt gcttctggca tgctcctttc ctatgttgca gattatttgc
    31021 taaaagggtt gcctgaaacg atggcttggc gcgtgatgtt agggctcgct gctgtccccg
    31081 cgctgatttt atttttcggt gttttagctc taccagagtc accgcgtttt cttatgcaat
    31141 ctggccgctt ggaagaggca aaaagagtat tgaactatat tcggacacca aaagaagctg
    31201 aacaggaatt cgaacaaatc cagctgaatg tcaaacaaga aaagacaacc ggaacttctt
    31261 ggcatacttt gtttttagaa aaatatcgtt ccttagtcat tgccggtatt ggtgtagcag
    31321 tattccagca atttcaagga gctaatgcta ttttttacta tattccgctg atcgtagaaa
    31381 aagcgactgg gcatgcagcg agcgatgcat tgatgtggcc gatcatccaa ggaatcattt
    31441 tagttgctgg ttctttgctc tttttggtta ttgcagacaa gttcaatcgg cgaactttat
    31501 tgaaaatagg cggtagcgtg atgggacttt ctttcatcct accagctgtt ttaggaactg
    31561 tactggatgc tcatacaaat tcgttgttga ttctcttgtt tttatgtatt tatgtcgcat
    31621 tttattcttg cacatgggct ccactgacat gggtaatcgt aggcgaaata ttcccactag
    31681 ctgtccgcgg gcgagcatct ggcctagcct cttcttttaa ttggattggt tctttcctag
    31741 ttggcttgct ttttcctgtg atgactgctt ctatgtctca agaaatcgtt ttcggcattt
    31801 ttgggatcat ctgcttttta ggggttttat ttattcaaga gatcgtacca gaaactcgtg
    31861 ggaaatcttt agaggaaatc gaacaaagtg catctaaaaa aacttatcct aaacgtattt
    31921 ctatccatca ttcttaaaga ggttgtgaca gaagtggtca gcttcaagaa ataagaagga
    31981 accgcttagt ttccagtcac aaggaacaat ttcaaaaaac agcttctcat gtttgatgaa
    32041 tattgtgact tgtgaccagt tggtaggaga acaccgttta gtggagaggt ggtgaaagaa
    32101 acgttcagcc ttgagcgatg acaaaagctc tcacaaacat tgcgacgagt aaccgtagga
    32161 gcaataagag gcggtaaaaa cccgtctctt atttttcgtt ttctttcact gtttcccgtt
    32221 caactagtac aggttcatag agaatatttg gctgtgcctg tcctttttca atcgttgtga
    32281 cgatccaccg tgcggcatct tgtcccaact tttcttttgg atgcgtcaat gttgtcaatt
    32341 ttttattccc taaatggctt aaagaagaat cgtctgttcc tacgatcgat aggtcatctg
    32401 gaatatgata gccattttct gtcaacttat ctagcattaa actagctatt tgatcgttat
    32461 agcaaatgat gccatctaaa gcttgttctt ctttcaaccg ttgtactgtt ttttcagcga
    32521 ttgtttctcg tgtttccgtg gtgaaagtaa tgatatcttc cgtcaaaaaa cgcacattat
    32581 actgttcaca cgcgctgata aaacctttca tcctgtattt tccttgaaga tcgtcgattt
    32641 ttgtgatgaa caacagacgc tgatgtcctt tttttatcaa atattctgct gccataaaac
    32701 cgactttcac atcatctaaa caaatataag aggcatttaa ctcttcataa gcagcattga
    32761 tcatcaccat cggaatgcct tgttctctca aagcaacata aagtgctaag tttgggttat
    32821 actgattact tttcgtaggc tcaacgatca aaccatctac cccataagca atcattttct
    32881 tcaaacactc tttctccaat tcgtgatcgt tattcgtact ggctaaaagc aatgaatacc
    32941 cagattctcg taattcattt tcgattcctc ggataatcga cggaaaaata taatcagaga
    33001 gatatgtggt gatgacaccg atcgtttttg ttctgttcaa tggtgttttt tctttcttat
    33061 agctgtcatc tacatatgtg cccgaccctt tttctttacg cagatagccc tcatttacca
    33121 acacagcgat tgcttgccga atcgtatgtc tgctgacgtc atatatttct tgcagcttga
    33181 attctgaggg aatggttgta tttacttcgt attcctcacg aaggatcttt tgttttatct
    33241 cttctgcaat atcttgatat tttgccattt gccttgcccc tttttatttg tacgtacata
    33301 tatcataacg agtctgcaag gtttattcaa gcaaaaatca aggtttcttg tgctcataag
    33361 gcatacggaa caggttcacc aaattctgga aatccttgct cattccacgt aaagcattgc
    33421 gcgttcgcgt gccgattagg attgtctaac ggatcccctt tttggttctt ctctggtcga
    33481 gcatgataga tcaaaatatc aaagcacccg tcttctgaaa ccgtaaacga gttgtgacca
    33541 ggaccaaatt gcttattttt ttcagaagaa acgaatactg gctccgataa tttatgccat
    33601 gattctttta ataaaagatt cttgtgctca tcagcccaca gcaggcccat ggcatagttt
    33661 tcatctgttg cactaccaga atacgtaatg aatattttcc cattgcgagt aaggactgct
    33721 ggaccttcat ttactgaaaa accccgtttt tcccattcat actcaggtat agaaaggagg
    33781 atctgcttcc cctttaacgt ccaaggatta tccatttcgg atagataaag attagaattc
    33841 ccagtaattt caggatcttg ttgggcccac acataatata gctttccttg agaagagaaa
    33901 gttgttccgt ctaaactgaa agtttccctt ttcgttttta tttgtccctt ttctatccaa
    33961 tctgtattca ttggatctat atgctgattt tcaagtacga acatgcggtg atgatgagat
    34021 tcatcacgta tagttttatg attgcttgca gcaaagtaaa tataccattt tccattgata
    34081 taatggactt caggtgccca gatcaattca ctcatcggtc cttcttcatg agcctgccat
    34141 acaatcaggc ttgcagcatg ttctaattca ttcagtgact ttgcccttct taattcaatc
    34201 acttggtagc ctggaaccga tcctgtaaaa taataataac catctgtatg tttatatacc
    34261 caaggatctg cccgttgccg aacgatagga ttattataaa gtgtcttcat cataacaaat
    34321 ttcctcacat tttctttatt ctaattacat tccaagagta tttttctatc cctacattta
    34381 attcgtcatg tttttctatt gaaaaatagt ttatttcagg ttttattttt tctggttctt
    34441 cacgtgtatt tttgtccaac agatcgctac tggttaatgt gtagtgctct ccttgatcta
    34501 gcaaattgaa atcatgttca atcgttatct tttgttgttc atttgtgcga ttgatcaaga
    34561 aaatagtcag ctgattgccg ttatctacga tcacactgtc cacataagga acatttttta
    34621 tcttttcaca atcataaaca gggcaaatga tttgttgttt caatacttga cctttagcat
    34681 atttcgataa gtgttgcatc acataataag ttggattcct ccatgcttgt cctttagcat
    34741 ctgtcagtat taaaggtatt gtgtttacta acaatgattg acaactaatc tttacgcgat
    34801 ctgcgtgtcg taaaatagcc agccctaatg aaccaaataa taatgtatct tccatagtaa
    34861 agtaacacca atcgatggga ctaccgactt caaaatccct atgtcttttt tccggttggg
    34921 catgtacatt ccattcatca aacgctagat acattgtttt ctcacttctc aaatatcctt
    34981 ttacataatc acaagtagca acgatttctt caatttgacg atcgaatcga taagcttttg
    35041 caaggtatgt cgctgtatca tcacgctcat ttttaggagg tttagtcaag tcttcttcat
    35101 catacttatc aatatagtta tgcaaagcta aataatctac attctcataa gcttcttcta
    35161 atgcgatacg atcccattca gggtacgatg gtaaacgtgg cgtagagctt ccaactaaga
    35221 ttgtttcaat tgaatcatcc actaacttca tgacttttga ggcttcattt gctaacctac
    35281 catattcata agcactctta gcccccactt gccatggacc gtctagctca ttccctaaac
    35341 accataattt aacgccaaaa ggcttttttt gcccattttt tattcttaaa tcactccaga
    35401 aggttccttt tggaaaatta caatattcta ctaaattttt tgcttcatca attccttttg
    35461 ttcctaaatt caccgtgaat atcggtttcg cttgtacatg actgatccat ttaaaaaatt
    35521 cgtggagtcc aaaagaattt ggttctatac ctcgccatgc taaatcaatt ttttttggtc
    35581 gttgttcttt cctacctaca gtatccaccc aatcataacc agatgtaaag tttcctccag
    35641 gatagcgaat gacacctaat tctaattcct ttacataaga gaggacatct tgtctaaacc
    35701 cgtcaccatc agctgtttca tggcccggtt cataaatccc tgtatagacg acacttccca
    35761 tatgctcaac aaatgagcca aacagccgag aactgatctc tcctaccgta aaatcctctc
    35821 tgatttttac tttcacttct tcgtccaaca gctttcccta cctttcattt cctatagtca
    35881 agataataga tacaagtttc catacgccac cagcaaagat aaaaataaaa ggagggaaaa
    35941 gaaatatgag cagtacaaat cctaataaca atacgcaaca aagaatattt ctcaaaaaat
    36001 caaaaaatac tttccatgca ctttgttcaa aaatttgtaa aaatgtgata tctttgtttt
    36061 cagaaatcac atatatccag gataaaagaa acatggaata aataagtaaa actacatatc
    36121 cactaattga aagtacgatt gtctgttctt ctaataattg attaacaaga aaataaataa
    36181 tgatcgttaa cagactaagg aacaagttct taccaaaatg ttgctttatt ccttgaaaga
    36241 aaaaataaat gatatttcct gatccctcac ctttccattg cgccattaca taaaaaacag
    36301 cacatgtagc aggcacgata gtaataacag gcaaacacac gattacccag agaataccta
    36361 ataagatgta atcagaaacc acaatcaact gtccataaag tttagtctca actattttca
    36421 tcttcctcac tctttcattc cagacatagc tgttccttga acaaaatatt tctgtgcaaa
    36481 aatataaacc actagtaatg gcagcattga aataaccgtt ccagccatta caggtccata
    36541 aaaagaaatg ttctgccctg taaataaagc taaccctgct ggtaaagttc tcatttttga
    36601 actacttgtc aatatcaatg gataaagcaa gtcattccaa ctattcatca gcgtgaaaat
    36661 ccctaatgat aaaagagtag gtttgctcaa tggcaacatg attttcaaaa aaatattaaa
    36721 ctcacttaat ccatcaatgc gtgctgcctc ttccaaagaa tctggtaaag taataaaaaa
    36781 ggaacgcatc atgaagatac caaatgcagt tgtcatacga ggaatgatca aacctgcata
    36841 agtatcaagc atgccaagca aattgacttc aataaataga ggaatcatga agacttggaa
    36901 tgggatcatc attgtcagca aaacgaaata gaacaaaact gattttcctt tgaaacgcat
    36961 acgagcaaat gcataccccg caagagaatc aaagaataca gaagtgatca ctgtccctcc
    37021 agcaaaaatc actgtatttt tgatgtaatc taataaaggg atcgttgtcc aaaccttaat
    37081 atagttatct agggtatagc tatctgcaaa tattctaggc ggaaatgaga taatgtcttt
    37141 ttcgtgttta aaagaagaaa taatcagcca caaaaaagga aacgcaataa ccaacaatat
    37201 aactagcata ctaatcgttc gaatgatttt tttaccacta actgcttttc gactattcac
    37261 agtaactgta atcgagcttt ctggtgccat acaggtttcc ctcctatttc atttgttttt
    37321 ctttccgtga catgtacaca ttagtggaga tagctatagt tgcaacaatg ataaacaaaa
    37381 ccgaagacag tgctgatgca tagcccaatt cataaggtgc tgtaaatcct cgactgtaaa
    37441 tatattgaac agctgtttct gtcttaaatt gtgggccacc agcggttgca acatatactt
    37501 ggtcaaacac ttgcatcgaa ccaatcagat tcgttaaaat acaaaaacct aaggagggaa
    37561 cgatctgagg tagcgtaatg ttaaaaaact gctgtcgatt tgttgcacca tccatttgag
    37621 cagattcata caaagaggga gagattccct gaatagcagt caagagaatc gtcatagtaa
    37681 caccaaaatt cttccaaaca gtcatgatgg ctaccgtcgg catagcaagg gttggatcgc
    37741 gaaaaaattg agggatcggc aagccgactt gtgaaaacaa gtaaggaatc gcccctacat
    37801 tcggatcaag caacatagaa aaaataatcg caattgacgt caatgaacaa ataactggaa
    37861 tgtaaaatgt cgaacgacaa aaacgattga atctggtatt tttagataat gctactgctg
    37921 ctaataaacc aacgataatt tgtgttggcg tttcgagtac ggtgaaatat aaagaatgaa
    37981 tcaacgaatt aactgctcgt tcatcactaa atatacgtgt gaaattttca aaacttacaa
    38041 aatttcttga tgtaaagaaa atattcaaat ctgtaaaact aatagcaaat gttcctaaaa
    38101 gcggtatgac agtaaataat aataaaacta aaaaagatgg agttaaaaac aaatacgcag
    38161 cacgatcagg acgattccaa tatttggtga atccgttcat atagtccacc tcatctttca
    38221 ttttgataaa atatacaatc ttggtttaaa cgaatctgaa taagatatcc gaacaatcgc
    38281 tgtgattttt gcaataacac ctatctgcaa atactaggtc gatcattcag atatctgtat
    38341 cttgccaaca gtctatcctg tataaagttc tgtactattt ttgcagtaat tgattgatgg
    38401 catcatctgc atcgttcatg agtgtctcag gatcttctcc tgctaataat ttttcaatca
    38461 gtggattgat gatatcgtta ttgatcgcag agatttgggt aaaacctggt aaatatggct
    38521 ctgcatagtc catttgtttc gataattcag atacaatagg atcatttttt acttcttcat
    38581 cttttgctac tgaatccaag taggcaggaa aaccattttc gatactccat tttttaccaa
    38641 tttcagttgt attccaatat ttcaaaaact catagacagc ttctttttta ttattatctg
    38701 tacttgccgg aatggcatat cctacgccat ctaaaatagc tgtttttttg tctgaatcac
    38761 tcccttgtgg taaggtagtc acattataat caatctcgtt tgctttcaat ccgttattta
    38821 accatggccc attgatttca atggctagtt gaccagcatt cattaaattg tccatttcgg
    38881 cgccagaaat cgattctgga ccagtcttgt ctacatggat cattttttgg atcgctttta
    38941 acgttttaag gttctcagga gacgcaaatt cagacttagt ataatcatcg ttcactaatt
    39001 taccgccatt tgctaacatc cagttatata acggtgcagt tccatcctta ttgaagacaa
    39061 aacctgatac atttttacta ctatcagtta attttggtgc catttctgtc aattcttccc
    39121 aagtagtagg tggttgttca gtatctaatc cagcttgttt aaacaaatca ttattccaat
    39181 acatatacat gccttgtacc tgcatgggga caaaatactg tttaccttct acttcaccta
    39241 attcaatagc cgtttcttta aaatcagatt tatctactcc atcatattcc cagaaatcgt
    39301 ctaatggttg taccgctccg ttttctacat actgtgcaaa atctggataa tttaatgcaa
    39361 taaagtcagg tgctttttta gaagaaatcg ctgtaggaag tttttcattc aaattggccc
    39421 aagtcatgat atccatttca attttgatgt ttttatcatt tgttttatta aattcattaa
    39481 cgatttcttg caatatttca ccatcagatg cagtaaaccc attccaaaaa gtcaaagtca
    39541 cttctttatc agaatcagat tctgctgaac ttccgccacc gcaagctcct aaaatacctg
    39601 ttgcagctac caacaaagag ccaattaacc attttttacc tttcattttt tgttcctcct
    39661 tcatctgata aacgacatct gaccacattc caagatagcg gtttcaataa acaattgatt
    39721 ttcccatctt cagcttttac atttgttgaa tgacttggtt ttacttgttc acttttagga
    39781 agattagttg cttttgtatc aaatccactc atttcagaaa aatcaatcac tccctctaat
    39841 gataattcag acaagtgaac agttagattc atttcttctt ctccacggtt aactgcaaat
    39901 atgacaagtt ctttgtcatt tattacggct atactttcta aatacggcac ttcttcaaaa
    39961 tcttccgtcg catagtttgc tgaacgaatt tgtgggctta acacctctcc ttgtccatac
    40021 acagcagctt gcataaatgg ataatagatc gtctgtctcc aagcttctcc gtttgtttct
    40081 gtcctgattg gtgcaataac atttaccaat tgagcaagac aagcaatctt tactctatct
    40141 gcatgtttca ataaagtgat taatagacag cctaatagta atgcatcttc aaaattatag
    40201 ttatcttcta gtaaaggagg agcaatctgc caaggcgctg cttgctcatc ttgttccgct
    40261 gaatgatacc aaatattcca ttcatcaaag gacaggttga tctgcttctt actatgtttc
    40321 ttcgccttga ctgtatcgca catcgctgtc acttcagaaa tgaaccgatc catgtctaag
    40381 gaacgtgcaa gataatgaga aagatcattt ttttggttgc cataatattg atgcagagac
    40441 aaataatcta tttgatcgta cgcttgatca agaactgtta actcccaatc accaaatgta
    40501 ggcatttgcc gattcgaact tccgcataaa accacctcaa ttgaatcatc tacgagtttc
    40561 atcgccttag ccgtttcctc tgctaaccgt ccatattctt ccgctgtttt atgtccaatc
    40621 tgccaaggac cgtccatttc attccctaaa caccatgtct taattccaaa agggctctct
    40681 tgaccattcg aacgccttaa atcactccaa taagttcctt taggaaaatt acaatattcg
    40741 actaaattac gagctgcatc tactccacga gtacccagat tgacagccat attgatttcg
    40801 gcccccacat gctgacacca gttataaaat tcatgaatcc caacttcatt cgtttcaatt
    40861 gtccgccaag ctaaatccaa ccttctaggt ctatttgcta tgggcccaat actgtcttcc
    40921 caacgaaatc ctgacacaaa atttccacca ggatatctaa tcatgggaat gttcaattct
    40981 tttaccaagt gaatgacgtc ttggcgaaat ccacttttat tagctgatgg atgatctggc
    41041 tgataaatcc cttcatatac tgctcgtccc atatgttcaa taaatgaact gtacaaccgc
    41101 ggatcaactt tgcctacacg cttgacacta ctaatcaaca ttgtggcttc cataactgac
    41161 ctcctttaat ttatacatga ataatacacc tcgttgaaag cgttgtcaat aatataaatg
    41221 ttaaacttac ttatttttta tcgattttaa ttaaccaaaa tattaataat gattaaaaca
    41281 aataatttaa tataactttt attaacaaat atgttaatat ataagaaaaa ccttttaaga
    41341 ggtgatatat atgcaactac aaataaacga agaatcctta ccggtatatg aagcattagc
    41401 tagtaaaaca aggatcaaga tcatccagct actttccaaa aaaaagatga atgtcaaaga
    41461 tttggcaaaa gaacttggag tgagcagtgc tatcacgacg atgcacgtga aaaagctaga
    41521 ggaagcaaac atcatcaaaa cagaaaaagt aggacaacaa aaaatttcga gccttcgtgt
    41581 cgataaaatc gatatcagtt ttccagaaaa aatattcaat gcttttgata cgaaagaaac
    41641 atcgatacct attggtcact atacaaatta tgcaattgaa ccaacatgcg gtttagctac
    41701 gatccatgat tttatcggga aagtggatga accacgctat tttatggatc ctcgaaggat
    41761 ggatgcccgc attctctggt ttaccagcgg atttgtcgag tatcaggcac ctaatttttt
    41821 aaatacagag gatacattgg agatgttgga agtttctgtg gaaatttctt ctgaatttcc
    41881 tttttcaaat gacaattggc cttcagatat cactttcagc ctaaatggtg tggagctagg
    41941 aacatggacg agccctggag attttgctga tatccgcggt aaatatacgc ctgattggta
    42001 tccagataat cttaatcaat acggcttgct gaagacgatc cgaattacca aacacttgac
    42061 gaatatgaat ggagaaccct tgtcaaacat cacgatcaac gacatcccta aagagcaaga
    42121 cacttggcat ttgcgaatcg aagtgaaaga cgatgccaaa catgtggggg gctgtacttt
    42181 atttggtaaa ggattcggta attacgatca ggatatcaaa ttaaaagttt actacagctg
    42241 atatttttct tttctatttt ataaaacaaa aacagccaat ccctatgaag ggacggctgt
    42301 ttttgcttta ttgttatttc cagaaatcat ccaaaacgga aattggcaaa tgtcgtttat
    42361 gttgtgtctt gttccaccat gcttcgattt tttcttgtgc ttgagcggga acttcttttc
    42421 cttctagata atcatcaatt tcattatatg tgacacctag tgcgacttca tctgcaatca
    42481 ttggtttccc atcttcaaga tctgcagtcg gaatttttgt gtataatttt tccggtgcat
    42541 tcaatgcttg aagcaattgt ttcccttgtc gtttgtccaa acggaataat ggcaaaatgt
    42601 cggctccacc atcaccaaat ttcgtgaaga atccagtaat gttttctgca gcatgatctg
    42661 tccctaatac ggctcctgct ctttctccag ctacggcata ttgcgtaatc atacgttgac
    42721 gtgctttgat atttcctttg ttaaaatcgc tgattcccac acctgcttcg cttaatactt
    42781 gtacttgtgc atcaacagct ggtttgatat ttactcgcag actaacatcg ggctggataa
    42841 agtcaagagc agcttttgca tcttcttcat ctgcttgttc accataagga agacgaacag
    42901 cgataaattg atagttttca tcttttgttt cttcacgcat ttcttccatc gttagttgcg
    42961 ccaaacggcc tgctaaagta gaatcttggc cgccgctgat acctagtaca aaagttttaa
    43021 gaaacggata ttttttcaaa tatgctttca taaagtcaat acttttacgt atttcttctt
    43081 ttggatcaat tgttggtttg acacctaatt cattgataat ttctgtctgc aggctcattt
    43141 attctcctcc tatgcttctg tatgaagttc tgctacgctt tttttcattc gttccatgat
    43201 agccatttta tggttccagc attcagtaga cagatcgact gggtattttt gcggattcaa
    43261 gtctcgttta tactcttccc atagtgaatc tagattttct tttgtatatt ctttgatttc
    43321 gtttagtgca ggtaattcgt aaactctttt tccttcgata aagatatctt gtaaaactgg
    43381 gcgagctgta aagtctcgaa cagttttatt gataaacgta tgcactggat ggaacatgaa
    43441 gatagcttct tcttctcgag ggtcttcatc ccataatgtc acgtagtctc cttcagattt
    43501 cccatcaaaa tttcgagtga tgcgccacac ttgttttttc cctggagtag ttactttttc
    43561 tgcattgctt gataacttga tcgtatccat catttttcct tcatcatctt cgatcgatac
    43621 tagcttgaat actgcaccaa gggctggctg atcataggct gtgatcaatt ttgtcccaac
    43681 accccataca tcaattttag ctttttgcat ctttaaattc aaaatcgttg cttcatccaa
    43741 gtcattcgaa gcatagattt ttgcttccgt aaatccggct gcatctaatt gttcccgaac
    43801 acgcttagat atataggcca tatctccact gtcgatgcgt acgccttgga agttgatttt
    43861 gtcacctaat tctttcgcca cgcgaatcgc acttggcaca cctgatttca acgtatcata
    43921 agtgtcgact aagaacacgc agtctttatg ggttttagcg taggccataa atgcttcata
    43981 atcatttcca taagattgga ctaacgaatg agcatgggtc ccgctagctg gtatcccaaa
    44041 tatcttccca gcacgtacat tgcttgttgc atctgctccg ccgatatatg ccgctctagt
    44101 tccccagact gctgcatcca gttcttgtgc ccgtctagtt ccaaattcca atagtggatc
    44161 ttctccgatg acagatttga tacgagctgc ttttgtcgcg atcaacgttt ggaaattgac
    44221 catattcaac agtgctgttt ctactaattg gcagtgcgct aaaggccctt ccacttggat
    44281 taatggttca tttgcaaaaa ctagttctcc ttcacgagca gaacgaactg ttgctttaaa
    44341 ttcaaaattt tgtaaatact ccaaaaaacc ttcaggatag acttctaatc cacgcaagta
    44401 agcaatatcg gaatcattga acgtcagatt ctctaaataa ttgactaacc gttccaatcc
    44461 tgcaaaaacc gcgtatccgt ggttaaaagg catttctcga aagtaacatt caaaaacagc
    44521 atgtaaatca gctcgaccta attcccagta tgtctgcatc atattgattt gatatagatc
    44581 cgtatgcagc gtcaaactgt cgtctgggta agtattattc atcttgtatt actcctcttg
    44641 gcaagttttc atttcctacc cattataaca aaaaaattaa taggaaaaga aatcttccat
    44701 tttttctgaa aaagcacatg ttttacggat atttcttgct aaaaatgaac tccatctgct
    44761 aaaactatcc tttcttattg gtatttaacc taaacgagaa agaatactta ttttattcaa
    44821 tgtatccgag acacgaagaa attagcgttt tctatttttc ttacttgata aaataagtag
    44881 attttcctac tacaatcgta gccgttaaga taccgatgat tgaacaaata taaccaaatg
    44941 tctctacaac ataatcaatg gcaaaagttt ttgaagcaat aatacgtctt tccttttttg
    45001 cttcttatcc ccaaagaaac ggatataact tattatgtat tgaatttttt tggaaaggcg
    45061 aattatgaca agatatacct tgcgttgaaa aaactcgcgg aaaaaatatc acacgttttt
    45121 aaaaagatac aaagtaagaa agcagctgtc aatagaatgg aaacaaaaat ggcgagacat
    45181 caatcagtct cgccttgttt tttattgtat acattagata tggagcaggt taccataaag
    45241 atgattatta gtcgttatta aatagaatcc ttcgtatttt tttgttctat cactacaata
    45301 tctttatttg gacgtttgaa ttgtattccg gcacgttcta gctcctgttt cactcctaca
    45361 tggactttga catcttctcg tgtcattttt aaaacgtgat gaatcgcata gtcgatttct
    45421 tgccttgtga tttttttctt cgtattgatc atcacgactt gctttttatc atatgtgtct
    45481 accaatagca gttgtgttcg atctaacgaa aataatttgt aataattaat agccgtattc
    45541 ccaaaaaaga attccactac tttaggataa aatgtcttaa tatttaaatt actattcaca
    45601 ggtgtttcga ttagcttcat ctcattcctc ttttctatat agtcgattta gatccaatct
    45661 taagttatgt taccatcttt cctagcatta aggaatcggt tacaattgaa cctttaaaga
    45721 caaaaggatt taccttttat cacttaatag tataacataa tttcgtgaga aaaaaataca
    45781 attttttctt gaccatctcg ttacgtaaag gattatgctt acatttgtca ggaggaaaaa
    45841 taatggagta cacaataaag aaaatgtcag agatatcagg agtcagtccg agaaccttgc
    45901 gcttttatga tgagattgga ctgctaaaac cagctaggat caattcttca ggttatcgga
    45961 tttatggcaa aaaagaagtc gatcgtctgc aacacatctt attttatcga acaatggcat
    46021 tcaagttgga tgatatccaa gaggtcttag acaatccatc ttttgaccac cagaaagctt
    46081 taatcaagca tcagcaaatg ttgctggaaa aacgggcaca aatcgatacc ctcttgacaa
    46141 ctgtccaaca gacacttgat atgtacgaag gaggaagaaa aatgagtgac aaagaaaaat
    46201 ttgaagggtt taaacaacaa aagttgaaag aaaatgaaga atcctatgga caagagattc
    46261 gacaaaaata cggaaaaaaa aaggttacag catccaatga aaaatggatg aatatggata
    46321 aagaaacgta tgaagaaatg caaactgttg aaaagcagat gttatttgat ctcactcttt
    46381 acttaaaaga accagaagat gaaagcttag cagaccgtat cttccaagct cataagaagt
    46441 ggttctctta cagctggcct gaatatcagc caaaagcgca taaaggacta ggaatgatgt
    46501 acgaagccga tgaacggttc actgcatatt atgacgagcg aagtggtcat ggtgcagcaa
    46561 aagctttaaa tgaaattatc caaaaaaagg cgtaatcttt tacatgatcc aacaaaaaag
    46621 tacacgtaaa atgacctaat aacaaaacag cagacaccca agttagattt ttatgctaac
    46681 ttttggtgtc tgctccattc tttcacaagt tttttcatcc tactttaaat atattcatcg
    46741 ttgaataaac gtataaggta ttttcacttt atcctttttt tctgtaagaa acaattgaaa
    46801 tgccatttct ccgcaagctt ccagatggtg gtcgatggtt gggaaaccaa gaagctcgct
    46861 gatcagcaaa ttatcgcgtc caatcaccac tggtgtctta tcttgcagat agctttgcaa
    46921 gatgcctgct gcaaccatat ccgaatttgt aaagacacca tcaactccaa gctctttaaa
    46981 aaagaagcct gcttgttttc ctttctctgc atctatacaa tcccaaaaaa caagtgattc
    47041 atcaaaagat ggaaaaagtt gatgacatag ctgcaaagtg atttttgagt tataacttag
    47101 cctgccactt cgccccaacg tgatgcccaa ttgctttata ccttgctgct tcaaataagt
    47161 aagaccttct gtaattgatt gttctcgatc aataaagaca cagcttgcct gttttccctt
    47221 gatttcttca caaaaaacta gaggtccata tttttgatat gccaataaat catctagtgt
    47281 attggcacga gacgtaatga tcaatccatc gaaagcttta gctgcaaatt cttcaagata
    47341 ccttcgctct ttttctttat gatagcccgt cggtaaaagt gtcactttgt aatcataatc
    47401 aaacgctgct tcaatgatcc cactgatcaa ctgatcaaaa tacggatgat tggtataagg
    47461 cagaatcact ccaatgcttt tcgtttttcc ataacttaac tgtctggcat ttgcattagg
    47521 gatataatcg acttctttca tgaccgcaag tatttctttc cttttttcct catctacata
    47581 tggatgtcca ttgattacac gtgaaatagt agaaactgaa taacctgtga gctttgcgat
    47641 atctcgaatg tttgccactg ctgcttcgcc acctatacaa aaagattttc ttgtaaatag
    47701 tctaccacag ctggaccgca ttcaaaaaac tgatacattt ttccttcatg cgaaaaactg
    47761 atccgctgcc cgccccaata tatatctttt gtcaaatttt cgatttcatt cattggtatt
    47821 tcttttacta tattcctatt caacccttga aaactgtaca acgttaatac ggatcccttg
    47881 aactcgacaa acgcataaaa ataattgttc gttgtgccat ctgttatctc tacataagta
    47941 ttcattgcaa gccctccttt tgatactttg agtatagtgt atgaaacgcg tctcatagca
    48001 aggagtttgt tttgccaaag aaaagtttta ttcctctttc attattacca aagtctctta
    48061 ttttaaattg aatacattat aatgatactg taaatatatc tttattatta gtgaat
//
LOCUS       DAZABD010000015        47731 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000015 DAZABD010000000
VERSION     DAZABD010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.015"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            52..2217
                     /gene="acm"
                     /locus_tag="ACHALG_000919"
     CDS             52..2217
                     /gene="acm"
                     /locus_tag="ACHALG_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HFN2586346.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(2489..2974)
                     /locus_tag="ACHALG_000920"
     CDS             complement(2489..2974)
                     /locus_tag="ACHALG_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HFN2586347.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    3064..3409
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3454..4095
                     /locus_tag="ACHALG_000921"
     CDS             3454..4095
                     /locus_tag="ACHALG_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFN2586348.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            4114..4749
                     /locus_tag="ACHALG_000922"
     CDS             4114..4749
                     /locus_tag="ACHALG_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFN2586349.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            4767..5582
                     /locus_tag="ACHALG_000923"
     CDS             4767..5582
                     /locus_tag="ACHALG_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFN2586350.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            5901..7337
                     /locus_tag="ACHALG_000924"
     CDS             5901..7337
                     /locus_tag="ACHALG_000924"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFN2586351.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            7526..9973
                     /locus_tag="ACHALG_000925"
     CDS             7526..9973
                     /locus_tag="ACHALG_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFN2586352.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            10203..11270
                     /gene="rlmN"
                     /locus_tag="ACHALG_000926"
     CDS             10203..11270
                     /gene="rlmN"
                     /locus_tag="ACHALG_000926"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFN2586353.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            11260..11466
                     /locus_tag="ACHALG_000927"
     CDS             11260..11466
                     /locus_tag="ACHALG_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2586354.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(11463..12566)
                     /locus_tag="ACHALG_000928"
     CDS             complement(11463..12566)
                     /locus_tag="ACHALG_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFN2586355.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            13119..13667
                     /gene="aac(6')"
                     /locus_tag="ACHALG_000929"
     CDS             13119..13667
                     /gene="aac(6')"
                     /locus_tag="ACHALG_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFN2586356.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            complement(13915..14034)
                     /locus_tag="ACHALG_000930"
     CDS             complement(13915..14034)
                     /locus_tag="ACHALG_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFN2586357.1"
                     /translation="MVFQKPLNNFRYIVYYCHTKIKKNKQKETVTKVLSQSLI"
     gene            14213..15874
                     /locus_tag="ACHALG_000931"
     CDS             14213..15874
                     /locus_tag="ACHALG_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFN2586358.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(15944..16291)
                     /locus_tag="ACHALG_000932"
     CDS             complement(15944..16291)
                     /locus_tag="ACHALG_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HFN2586359.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            16375..17316
                     /gene="opp3b"
                     /locus_tag="ACHALG_000933"
     CDS             16375..17316
                     /gene="opp3b"
                     /locus_tag="ACHALG_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFN2586360.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            17317..18360
                     /locus_tag="ACHALG_000934"
     CDS             17317..18360
                     /locus_tag="ACHALG_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN2586361.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            18366..19436
                     /locus_tag="ACHALG_000935"
     CDS             18366..19436
                     /locus_tag="ACHALG_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN2586362.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            19426..20379
                     /locus_tag="ACHALG_000936"
     CDS             19426..20379
                     /locus_tag="ACHALG_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN2586363.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            20391..21401
                     /locus_tag="ACHALG_000937"
     CDS             20391..21401
                     /locus_tag="ACHALG_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFN2586364.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(21686..22507)
                     /locus_tag="ACHALG_000938"
     CDS             complement(21686..22507)
                     /locus_tag="ACHALG_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFN2586365.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(22604..22882)
                     /locus_tag="ACHALG_000939"
     CDS             complement(22604..22882)
                     /locus_tag="ACHALG_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2586366.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            23150..25159
                     /gene="metG"
                     /locus_tag="ACHALG_000940"
     CDS             23150..25159
                     /gene="metG"
                     /locus_tag="ACHALG_000940"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFN2586367.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(25638..26225)
                     /locus_tag="ACHALG_000941"
     CDS             complement(25638..26225)
                     /locus_tag="ACHALG_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2586368.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            26400..27722
                     /locus_tag="ACHALG_000942"
     CDS             26400..27722
                     /locus_tag="ACHALG_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFN2586369.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            27877..28653
                     /locus_tag="ACHALG_000943"
     CDS             27877..28653
                     /locus_tag="ACHALG_000943"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFN2586370.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            28646..29215
                     /gene="rnmV"
                     /locus_tag="ACHALG_000944"
     CDS             28646..29215
                     /gene="rnmV"
                     /locus_tag="ACHALG_000944"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HFN2586371.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            29208..30092
                     /gene="rsmA"
                     /locus_tag="ACHALG_000945"
     CDS             29208..30092
                     /gene="rsmA"
                     /locus_tag="ACHALG_000945"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFN2586372.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            30543..31172
                     /locus_tag="ACHALG_000946"
     CDS             30543..31172
                     /locus_tag="ACHALG_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2586373.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(31296..32405)
                     /locus_tag="ACHALG_000947"
     CDS             complement(31296..32405)
                     /locus_tag="ACHALG_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN2586374.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            32614..33036
                     /gene="mgsA"
                     /locus_tag="ACHALG_000948"
     CDS             32614..33036
                     /gene="mgsA"
                     /locus_tag="ACHALG_000948"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFN2586375.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     regulatory      33223..33321
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            33359..33895
                     /locus_tag="ACHALG_000949"
     CDS             33359..33895
                     /locus_tag="ACHALG_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFN2586376.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(33935..35698)
                     /locus_tag="ACHALG_000950"
     CDS             complement(33935..35698)
                     /locus_tag="ACHALG_000950"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN2586377.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(35701..37416)
                     /locus_tag="ACHALG_000951"
     CDS             complement(35701..37416)
                     /locus_tag="ACHALG_000951"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN2586378.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            complement(38195..38419)
                     /locus_tag="ACHALG_000952"
     CDS             complement(38195..38419)
                     /locus_tag="ACHALG_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2586379.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            38610..41387
                     /locus_tag="ACHALG_000953"
     CDS             38610..41387
                     /locus_tag="ACHALG_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFN2586380.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            41468..41887
                     /locus_tag="ACHALG_000954"
     CDS             41468..41887
                     /locus_tag="ACHALG_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFN2586381.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            41899..42369
                     /locus_tag="ACHALG_000955"
     CDS             41899..42369
                     /locus_tag="ACHALG_000955"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFN2586382.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            42386..43165
                     /locus_tag="ACHALG_000956"
     CDS             42386..43165
                     /locus_tag="ACHALG_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFN2586383.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            43152..43973
                     /locus_tag="ACHALG_000957"
     CDS             43152..43973
                     /locus_tag="ACHALG_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFN2586384.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            43990..45039
                     /locus_tag="ACHALG_000958"
     CDS             43990..45039
                     /locus_tag="ACHALG_000958"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFN2586385.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            45052..46056
                     /locus_tag="ACHALG_000959"
     CDS             45052..46056
                     /locus_tag="ACHALG_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFN2586386.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            46689..47708
                     /locus_tag="ACHALG_000960"
     CDS             46689..47708
                     /locus_tag="ACHALG_000960"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFN2586387.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT        15801 a         8305 c         9854 g        13771 t
ORIGIN      
        1 gagatgatat agtaggtttt atttttagtt aaaatagggg agatagaaaa aatgaaaaag
       61 tgtagaaata ttattttatc aatgttattt attattacta atataacatc actgattcct
      121 gttcatgtct atgcggatgc aggcagagat atcagcagta atgtcacttc gttgaccgtg
      181 gacccaacga atattacaga tggaggaaac ataaaagtaa aattctcgtt tgacgaaaaa
      241 aaacaaaata tccaaccagg tgattatctt tggatcaatt ggccaagtga aggaaatatt
      301 cgcggtgaag gattccaaaa agaaatccca ttaatgattg aaaataaaaa tgttggaact
      361 ttaacggtca gaaaagattc tgcgcaggtt gtgtttaatg aaaatattaa aaacctagac
      421 tcagtagaag gttgggggca atttgagata caggccagaa acgtaaccga taccggtgaa
      481 gaaaatacgg gaccattcac cgtgacgagc ggtgataaaa cagctactgt aaatgtgaca
      541 aagccagctt ctggttcttc ttctagcgtt ttttattata aaacaggaga catgctacct
      601 gaagatacta agcacattcg atggtttttg aatattaaca ataacggcac ttatgttgaa
      661 caaccagtta aaatcagtga tgaaatccaa agtggacaaa gattagatcc aagtacgttt
      721 gaaattaatc aaatacactt aggtgaacag aaggtttata gaggagaaga gggaatccaa
      781 caatttttac aagatttccc cagcgctaca tttaattttt cagtaactga taattacatt
      841 gaaataacta taccaaaaaa ctttgtcaat cttaggaaaa taatggtgag ttataagacg
      901 atcatagaaa acccagaaca aataaatttt gagaatcatt cggaagcttg gtttaaagaa
      961 tttaataaac ctgctgttga tggagaaagt ttcaatcata cagttaaaaa tatttcagct
     1021 tctggtggag tcaacggtac agttagaggc gaattgaaga ttttcaaata tatcaatgac
     1081 acagagattg gtatcccaaa tgtgactttc gaattaagac gagcagatga acagcctatt
     1141 caaggacagt ctagtatcct gttgacttcg aatgaacagg gagaagctag tatcaaaggc
     1201 ctgcaagtag gagactatgt tgtaaaagaa aaagaagcac ccaattggat tgattttgat
     1261 ccactaagtt caaatgaact caaattctcc ataaacgaaa atgatacaga gggagtcagt
     1321 ttacctattt ataacaagaa aaaggttacg aacatcacag caactaaaat ttggaacggt
     1381 ggaaccactc caagaccgag tatttatttt aaattattca gagcaagtac aaacaattgg
     1441 gaaccagttc ctgatgcaga aacaaaacga ttagacaatg gaataacttc agtgacttgg
     1501 gaagatatcc aacagtatga tgatagtgga aacgaatata cgtttaaagt gcaagaagta
     1561 gatcaaaacg gaaatgatta tgtcccttca ggttatcaaa aaattgaaaa cgggctagtc
     1621 gttacaaatg agaataaaga agtcatttca gtctcaggac aaaaaacatg ggaagacaat
     1681 gagaaccaag acggcaaacg accgacgacg atcacagtaa atctattagc cgacggtcaa
     1741 ccgatccaac acaaagaagt aagtgaaaaa gatgattggc gctatcgttt cacaaatctg
     1801 ccaaaataca gagacgggca agaaatcatc tatactgtaa cggaagacaa cgtgcccgaa
     1861 tacagtacga cgattgacgg gtataacatc aaaaacagtt atacaccgga aaaaacaaat
     1921 atatctatac caggtagcca tataacaccg tggaaaaatt ttccagagaa gacagagaga
     1981 gagaaaatta gaaatctatt ctctcaaaaa ttgcaaatat ttgctcatac aggagaatcc
     2041 agcgaacaaa gaagcaatta tattagtaag agagctgtac ctaaacagat aacaaataaa
     2101 tcaaaaagca ttcttcctaa aacaagtagt aaaaaatcta gttttgctgt aatcattggt
     2161 ttattgatag taactatctg tgctgggata ttcttacaaa aatataaaaa agtttagtta
     2221 gaatataata ttttttgtta aatatgactg aaatcaaaag aataaaattc cgatttataa
     2281 tcgaaaaaag aagcattttt atatatgctt cttttttcgt agaatcaata aatatggatt
     2341 tggctaacta tggaagaata ctcgtattag ttttcagcgt tcatactagt tgttacattg
     2401 tttgattggc caaaagaatt tctgatatat tttgtgctaa ataaagaaaa agacgcttga
     2461 attgggaaat aagaaaacta ctctatcatt acaaattttc ataaactggc agatgttcta
     2521 agtaaacaag tatcttttca aagtcggaaa aatccataat caacgtagtg gtattgatat
     2581 ttggatgaaa gcctacggta tcttgacgat caatagcagc atcgatcacg acttggattt
     2641 ttttctcttt gtcgtgcatc aatgctaatg gagtcactgt acctgctgga acgcctagga
     2701 ggtcaggaag ctgttcttca gaaacaaaag ataagcgctt ttcttttaat tgttcagcta
     2761 gttgttttaa atcggcgtgc ttttcatcag gcaagatgac aagatagatt tgttttcctt
     2821 ttcttgattt caataataga ttttttactt gcggaccagg tagggaaaaa gggacatttt
     2881 tgactgacgt aatcgcagga tgatcgactt tttgaaaaga gatacccagt ttttttagta
     2941 gttcataagc ttcttgttcg gttgcacgac tcatcgcgca tcaccctcct ttttttatta
     3001 gtgtatcata aaaaagagtt gacaaatgaa aaataatcat gtaaattaca cgtaaagtta
     3061 aaacaaatga catcgaaaag aagagtagcc gttaggtgtt tttagagagt ctctggttgg
     3121 tggaaagagg cagcagttaa ccgtgaaaca catcttggag tcgattttct gaagcgtata
     3181 gtaggaaaat ccggggagcc cgttacagct gcggtcgttg ctagatgacc agataaggct
     3241 ggcttttccg tgaggagtcg gtaaaaaaag gtggaaccac gtgattaacg tcctttgatc
     3301 ttcggatcaa aggacttttt gttttcatct gaacggcaac gactgtgtga ggttttttct
     3361 gcaaggaaaa aataaacaga ggtggaacca cgtataatac gtcctcttgg catttgccaa
     3421 gaggattttt ttattttaaa ggaggaaaag aagatggagt atttgattga agttatgccg
     3481 caattattag taggagcact cacaacattg aaattatttt tatttacctt gattggctca
     3541 ttaccattag ggattgtttt agcattagga ttgtctttgc attttaagcc gctacgctac
     3601 ttgcttcaaa catatgtatg ggttatgcgg ggaacaccgc tgttgttgca actgattttt
     3661 attttttatg ggttgccgac gattgggatc gtttttgatc gtttcgaagc agcactcatt
     3721 gcctttatct taaactatgc agcttatttt gcagagatct ttcgtggagg gatacaatcg
     3781 attcctcaag gtcaatatga agcagcgcaa gttttgcaat taagtccctt tcaaacggtg
     3841 acgaaaatcg tcatcccaca agtgacaaag attgttttac cttctgtagg gaatgaagtt
     3901 atcaacttgg tcaaagattc ttccttgatc tatatattag gtttaggtga cgtaatgcgt
     3961 gctgggaaaa tcgcgatgga acgcgacgcg actttactgc ctttgctagg cgtggcaatc
     4021 atctatctta cattgactgg tattctaaca atcgtaatga aacaagttga aggaaaatta
     4081 aattattacc gctaactgat aggaggaaga aatatgattg aattgaagaa agtgaacaaa
     4141 gcatttggtg aaaaaaaaat aatcaaagat ttgaatctaa tcattccaga tgggaaaaca
     4201 ttagctatcg taggtccttc cggtggcggt aaaaccacgc ttttacgtat cttggctggc
     4261 ttagaaagtt ctgacagcgg agaattttat ttagacggtg taccgttcaa cccattcgag
     4321 cttaaaatga aagaacaagt tgtaggagtc gtattccaag acttccagct atttccacat
     4381 ctgaatattt ttgaaaacat taccttagca ccaaaaatgg tattgaaaca gacaaaagaa
     4441 gaatacacca aaaaagcaga agaattagcg gcttcattag ggattagtga tttattgaat
     4501 atctatcctt atcagctttc tggcggacaa aaacagcggg tagcgattgc cagagcattg
     4561 gctatgaatc cacgggtatt ggcttatgat gaaccaacaa gtgctttaga tcctgaatta
     4621 cgccagcaag tcgagcgttt gatcctatca ttgaaagaag atggggtaac ccaaattatt
     4681 gtcacccatg atttagtatt tgcagaaaat gtcggtgatc aattactaaa agtttcccca
     4741 attcattaag aaaggagaag atagagatga aaaaaaccag ttttctattc gctttgatcg
     4801 caggtcttct tctttttgca ggctgttcga acaaaaaaac ttcagcagat aattgggcga
     4861 ctatcaacga agagaaaaaa atcgttatag gtttagacga tacatttgtt cctatgggat
     4921 ttagagataa agacgggaag ctcacaggat ttgatattga tttagccaaa gcagtctttg
     4981 aacaatatgg gattacggta gactttcagc ccattgactg gtcaatgaag gaatttgaac
     5041 tgaacaatgg aacgatcgat ttgatatgga acggctattc caaaacgccg gcaagagaaa
     5101 agaaagtgca atttacaaaa ccttatatgg aaaacgatca agtgctgatc acacctaaag
     5161 aatcaggaat cacaaacttt ggacaaatga ataataagag attaggtgcg caaaatgggt
     5221 catctggtta tgatgtattt acgaaacaac cagaagtatt aaaagagata gtcaaaaacc
     5281 aagatgctgt tttgtatgat agttttaatg aagcattgat cgatctgaaa agcagacggg
     5341 tcgatggtct gctgatggat aaagtatacg cagactatta tttgaaacaa agaaacgaac
     5401 tagaagactt caacataaca aaaggttctt atgagagtga ggattttgca gttggtgcac
     5461 gcaagtcaga tagtacatta gtgaaaaaaa tcaatgaagc cctagatcaa ctagagaaaa
     5521 caggtgaatt ccaaaaaata tccgataaat ggtttggcga agatgttacg ccgagaaatt
     5581 aacaaatagg ggactgtgac aaaagacgtg tcacagtccc tttttccgaa taaacagtgt
     5641 tctcctgcca actccttgtg actcggagct gaatggcagg ctcacaacct cttggtaaac
     5701 ggtgttcaaa agccagtttt tcggtattaa gccaaattta tgcaaaaatg tagaaagcca
     5761 ttttcacaaa atttttctta ctactcgaaa ctaagcgact ttttcacaac ctcctttaca
     5821 tatttttatt ttgttgtgga aggggtttcg tgttattcta aaagaggaac gattgaaggt
     5881 tataggaaag gaagagatga atggaacaca aagaacttac ctcatttcca aaagattttc
     5941 tgtggggctc tgcttctgca gcgtatcaag tagaaggagc ttggcaagaa gatgggaaag
     6001 gagaatcagt ctgggatcga tttgtacgga tacctggaaa gacatttaaa ggaacgaacg
     6061 gtgatttggc agtagaccac tatcatcgtt ataaagaaga tattgcttta atgaaagaac
     6121 aaggtctgaa agcttaccgc ttctctgtag cttggacacg gatttttcct aatggacgag
     6181 gagaagtcaa tcaagctggg ctcgcttttt atgagagatt gatcgacgaa ttgattgaaa
     6241 acgatattga acctatgttg actttatatc attgggatct tccacaagca ttgcaagatg
     6301 aatataatgg ttgggaatca cgtcagatca ttgaagattt cactcattat gcagaaacat
     6361 tatttgaggc attccgcggg aaagtccatt actggattag tttgaatgaa caaaatatct
     6421 tcacttcatt aggttatttg ttagcagcac atccgccagg tgtgacagac ccgaaacgaa
     6481 tgtatgaagt taaccatatt gctaatttag caaatgcatc tgtcatcaat aaatttcacg
     6541 agttagaaat gcctggaaaa atcggaccaa gctttgcgta tacgccaaat tatccaattg
     6601 acagtaatcc tgaaaatgtt ctggctgctg aaaatgcaga agacctgatg gccaattatt
     6661 ggatggatgt ctatatgtgg ggaaaatatc cgattgctgc catgaggttc ttagaagaaa
     6721 aaggatgggc accaacgatt gaagctggcg atgcagaatt gctagaatct gctaaaccag
     6781 attttcttgg gatcaattac tatcaaacgg cgacgaatgc attcaatcca ttaaacggag
     6841 ttggtgctgg aaaaatgaat acgacaggaa aaaaaggttc atcagaagaa actggagttc
     6901 ctggaatgta taaaaaagtt gagaatccat ttgtcgaacg aacaaattgg gactgggaga
     6961 ttgacccaga aggattaagg atcggactgc gtcgtatcac aagccgttac cgcatgccgg
     7021 tactgattac ggaaaatggc ttaggagagt atgacaagtt gacagaagat aaacaaatcc
     7081 atgacgacta tcgaatcaat tacttacaaa gccacgtcaa agccatcaaa gaagcaattt
     7141 cggatggagc agaagtccta ggctattgca cctggtctta tacagatttg cttagctggc
     7201 tgaacggtta tcaaaagcga tatggatttg tatatgttga tcaagacgaa acacaagaag
     7261 gatctttgga acgatataag aaagacagtt attactggta ccaaaaactg attgaagaaa
     7321 atggagagaa cctttagttc acatacttaa aacaagaagg tacggctgga tatggccgtg
     7381 ccttttcttg ataaaatcat ttagaaatta agaaaaactg taaaataagc tgaaaaatga
     7441 aagaaagcgt cagttctttc aaacaaaact ttagttccct tacttttttt tgtataatga
     7501 aacttgatga aatgagggat aataaatggc acaaaagaaa cggcctacta aaaaaaggaa
     7561 aaagacgaaa aaaacgaaaa agcaaaaaca gcagcaggaa caaatgctga cgattggtgt
     7621 tgggtttgcc ttgattttat ttgctgtctt cggcttttta aaattaggtt ttttaggaat
     7681 attgattgca aacggctttc ggatcattgc aggaaatact taccaaatcc tctgtctttt
     7741 attagccgta ttaggatttt ggattgtcat taagaataca gagttttcaa ttggtaaaaa
     7801 ccgacgctgg tttggcggca tactctttta tttcggtatc ttattattgt tacatgccca
     7861 tttatttggc aaactccata ctggggaacc aaatatcatg gggacgacat gggatttgtt
     7921 agccagtgat ataaaacaaa gtcaagtaga caataacgtt ggtggtggga tgattggagc
     7981 aatcctttat cattttactt acttcttgat tgcacagcct ggaagttatt tagcagcagt
     8041 gctattactg gcaggaggag cattcctgtt cagtaatctg gaaggctatc agctgttaaa
     8101 tggtatccaa tcaattggag aacgagtaca ggaattattg gaaggtgatc ctgccaagca
     8161 agctcgaaag caagcggcaa aggaagagag aatgaaacag cgcgcagaag cgaaagaagc
     8221 aaggcgctta gctgcaaaag aagctgcaga aaaagaagct gcagaatatg agaagaaaaa
     8281 agcggtgagc cagcaaaggg aaaatactgc tgatgaatgg caggaaccag aaaatacaga
     8341 gccggagcag ctaagttttg ttccaatcga cagttttcaa gaaaatatcc atcctgcgaa
     8401 tctagagaaa ccagtaccag atacaccaaa acagaccaat acagcagaag gtttttcaga
     8461 tgagttgcca gaggatgacg gtacctcatt agaatttgag attgaagcag aacaggaaaa
     8521 tcaagactat gaattaccga cagttgattt gcttgattct ataccaactg tggatcaaag
     8581 tgatgagtat aaaaaaattg aaaaaaatat cggcgtatta gaacaaactt tccaaagttt
     8641 cggtgtcgat gccaaggtcg tcaaagccag cttaggacca tccgtcacaa aattcgaagt
     8701 ccagccggct gtaggggtaa aagtaagtaa aattgtgaac ttaacagatg atatcgcgct
     8761 tgctttagcg gcgaaagatg tccggatgga agcaccgatt ccaggtaaat cactgattgg
     8821 tattgaagta ccaaatggaa agatcagtat ggtttccttt agagaaatca tcgaagcaca
     8881 accaaaccac ccagataaat tactagaagt acctttagga cgtgatgttt ctggacgggt
     8941 acaaacggct gatctttcga agatgcctca cttactcgtg gctggttcga ctggtagtgg
     9001 gaagtcagta gcaattaatg ggattatcac aagtatatta atgcgtgcta agccccatga
     9061 agtaaaactg atgatgatcg atccgaaaat ggttgaattg aatatgtaca acggcatacc
     9121 tcatctgttg acgccggtcg tgacgaaccc tagaaaagca gcgcaagcct tacaaaaagt
     9181 agtacaagaa atggaagaac gatatgaaaa attcgcggca accggagtaa gaaatatttc
     9241 tggttacaat gaatttgttc aacagaaaaa cttggaaaac ggaacgaaac atccaacctt
     9301 accattcatc gtagtgatcg tagatgaatt agctgatttg atgatggtag ccagcaatga
     9361 agttgaagat gcgattattc gtttagcgca aatggctcgg gcagcgggga ttcatatgat
     9421 cctagctact caacgtccaa gtgtggatgt tatcacagga atcatcaaag caaatgtacc
     9481 ttccagaatg gcatttgcgg tatcaagcgg tacagattct cggacgatca ttgattctaa
     9541 tggggcagaa aagctcttag ggcgaggaga tatgctattc ttgccaatgg gtgagaataa
     9601 accgatccgt gtgcaaggag cattcatttc tgaccacgaa gtagaacggg tcgttcaatt
     9661 tgttacagat cagcaagaag ctcattatga agagaaaatg atgccgacag atgaagttga
     9721 aacgacaggt gctccagaac agccacaaga tgagctgttt gaagaagcaa aagcacttgt
     9781 cgttgaaatg caaacggcaa gtatttccct tctccaaaga agatttcgga tcggttacaa
     9841 ccgagcagca cgtttagtag atgaactgga agctcatgga gttgttggcc cgtcagaagg
     9901 aagcaagcct cggaaagtat tcattgagca ggaagaaaca tcagaaacgt tgccggacga
     9961 taatgacatg taaaacttgt gtgatgaagg gttgaaaaac gaataaccaa taaaaagaca
    10021 aggcatgatg ataccaaaag aaaaaatggt ttttttccat cttttctaat acggtatgat
    10081 gtcttgtctt tttagttttt gtgagattaa agttctattc gatttatttg tgaagtattg
    10141 cattaaggaa atgaatcatg tatagtatgt agcgaaactt tgagaaaaag agggaaaata
    10201 ctttggaaaa acagtccatt tatggcttga cgaatgaaga attaatcaat tggtttatag
    10261 aaaacggcga gaaaaaattc cgagctgccc aagtttggga atggttgtat caaaaacgtg
    10321 taagtaattt cacagaaatg acgaatctat cgaaacaatt gatcgaaaaa ttatcggagc
    10381 attttatcat caatcctcta aaacagatgg tcgtccaaga agcttcggat ggtacagtaa
    10441 aatatttgtt tgaactccct gataaaaaca tgattgagac tgtactgatg agacaagaat
    10501 atggcttgtc agtttgtgtg acgacacaag taggatgtaa tatcggctgt acgttttgtg
    10561 caagcgggct gctcaaaaag aaccgtgatc tgactgctgg agaaattgtt gcacagatca
    10621 tgatggtcca acattatttt gatgagcgca atcttggcga acgcgtttca catgtcgttg
    10681 taatggggat cggtgagcca tttgataatt acgacaatgt aatggacttt ttgcatatca
    10741 tcaatgatgc caaaggattg gcaattggtg ctcggcacat tacagtttcc actagtggat
    10801 tagcacacaa aatcaaggaa ttcgctaata acggcttgca agtcaatctg gctatttcct
    10861 tgcacgcgcc gaataatgag gtgcgaacat cgatcatgcg gatcaaccga agtttcccaa
    10921 ttgagaaatt gatggaagct gtggatgagt atttagagaa aacaaaccgt cgaatcacgt
    10981 ttgaatatat catgttgaat caagtaaatg atagaccaga gcatgcgcaa caattagcag
    11041 atctattaaa agataagaaa aaattagcat atgtcaactt gattccatat aatccagtca
    11101 gtgaacatga tcaatattct cgcagtccaa aagctgatgt attgagattt tatgatgtat
    11161 tgaagaaaaa cggcgtaaac tgtgtgatac gtaaagaaca tggtacagat attgatgcag
    11221 cttgcggaca attgcgaagc aagcaaatga aaaaaacagt tgccaaataa tcgtatccta
    11281 ctcgtttttt caattaccat aaaaggagca gttttcttaa ttaaggaact gttcaactta
    11341 aataagagaa aagattctat agcgaaagct ataaagcttt ttttgacaag aaaaaccctc
    11401 atcaagcaaa gaaatgtcct caatttgatg acagcctgct tgatgggggt tttagcaatt
    11461 atttaactga ttttcttata tccttgctgg ctttcttgtg tagcatgata agtttctacg
    11521 tcaagaatgg cctgtttaaa tttttctcgg gtgatttcat aaggcacatg tttaatatca
    11581 tttgttgcca tggctttatc taagattgct tctaaatcgg tcgtgacatt gagattcatg
    11641 tcggttaacg atactggaag ttggttcttt ttcataaaca catagatttt atcaaacaaa
    11701 tcgaactgct gatccatcat caataaggtc agtacaccat aactgacaga tgctccgtga
    11761 aggtatccct ccattttttc tagaacagtc gtaccgtaat agaatgagtg tgccatagca
    11821 gaattatagt tattgttaac taaaacagaa accaatccgg tagtaacaat gatatccgaa
    11881 acgacttgtt caattgcata tgagggacga tcgtcggcac tatctttcaa tgcttgcgta
    11941 ccataagtca agagacgatc aatgcacatt tttccgattt ggattcctaa tctatccgtg
    12001 tagcctagat catcgccacg agcagaaaaa gtagattcga cttctttgga taaagcatcg
    12061 ccaatacctg cccataagta tttatcaggt gcttcggcaa taattgctgt atcaatgaag
    12121 gtgtgtacag gtggttttga aggaaagaag agtccgtcca ttacgccttc ttcgttatag
    12181 atgacacata ccgcagtgac gggcgaacag ttcgaaccaa tagtgggaaa ggtgaagact
    12241 ggtttagcta gtttttcacc aatgactttt acagtatcgc aagcacggcc accaccgact
    12301 gcaaacagga cgtctgcttt ttggacactt tcatgctgga tcaactgttc aatatttcca
    12361 taagttgaat tacccccgta ccagagactg gcgacaacct ctatttcctg tgtcaaagct
    12421 acttttattt tgttttctgc ctttgaaagt gcagtcttcc ctccaatcag cactactttt
    12481 gtcccatatt ctttgacgat tccatttgct ttttcatacg catctgttcc aatggtataa
    12541 tttggtaaga aaacactttg actcatattc atttccttct ttcttcgttg aaacgggtta
    12601 aaattttaaa gagtgatcga tctgactgcg gctgtagatg gagtagaggt gaacctaaga
    12661 caggataagt gatgaataag gtggcatgaa caccttgaaa tcatcaataa gtatcctaaa
    12721 aacgaacaag tacaaaaaca ttgaaaacga caataatgaa tttttgttcc tcttagtatt
    12781 tctaccgatt ccctttgctt ttttgtaatt atctaaaaat tctaaaaatt aagattatta
    12841 tatgaaaaca ggttttattt gtcaattcat aattggtttt ttttaaagca aaagataatc
    12901 tatatcatga tgaacaaaca gagttttttc ctccaattga tggctttcct gatctttcct
    12961 attttgaata aagtgtttct gtataatttt tttattttat aagtaagata aataatgaaa
    13021 gaatagagat actgattggt agctaaagat ttttgagcga gaacatttcg ttttttcaaa
    13081 aggactgtta gaataagcaa gaacaagggg gaagacgtat gataatcagt gaatttgacc
    13141 gtaataatcc agtattgaaa gatcagcttt ctgatttact gagactgact tggccggaag
    13201 aatatggaga cagctcggca gaagaagtag aagaaatgat gaatccagaa cgaatcgcgg
    13261 tagcagcggt agaccaagat gagttagtag gatttattgg tgcaatccct caatacggta
    13321 tcacaggttg ggaattgcat ccattagttg tagaaagctc ccgacgaaag aaccaaatag
    13381 gtactcgatt agtcaattac ttagaaaaag aagtagcttc cagaggagga atcacgattt
    13441 atttaggtac ggatgattta gaccatggaa caacgttaag tcaaacggac ctgtatgagc
    13501 atacatttga taaagtggct tccatccaga accttcgtga acatccgtat gaattctatg
    13561 aaaaattagg ttataaaatc gtaggtgtct taccaaatgc aaatggctgg gacaaaccgg
    13621 atatttggat ggcaaaaacg attattcctc gaccaaattc tcaataataa aaagaggttg
    13681 tgacagaagt aatcagcttc aagaaataag aaggaattta cgaaaattac ttcttgaatt
    13741 tttgtgaaat tccaacttat ttccgaagaa gctacttttg gaacaccgtg taccagaggt
    13801 tgtgaaaaag tcgcttagtt tcgagtagta agaaaaattt tgtgaaaatg gctttctaca
    13861 tttttgcata aatttggctt aataccgaga aactggcttt tgaacaccgt ttattcagat
    13921 aagggactgt gacaagactt ttgtcacagt ttctttttgt ttattctttt ttatctttgt
    13981 atggcaataa tagacaatat aacgaaaatt atttaatggc ttttggaaga ccatggaaaa
    14041 ccttgtacaa agctattttt tctgaaaaat tccttgaaac ctcacgaaaa aatcattatt
    14101 ttatgaaaaa gacaaaaaaa agtgttgatt ttttaataat tattcattaa actcttaaac
    14161 aacttcagaa aatattaatt attctgaaaa ttccaataaa ggggtacgta aaatgaaaaa
    14221 gaaatttaca tttggtttgg ttgcggtatg cggccttgta cttgctggat gttatggcgg
    14281 cagttcagcc acaagcagta gttcagcaaa aagcggttca gcagatggag gaggcgtatt
    14341 taacttagtc gtgcctcaag aaatgcctac agctgactta tcagttgcaa cagatacaat
    14401 cagttttaca gcattgaata acgtttatga aggaatttac cgtcttgatg aagacagcaa
    14461 accacagccg gcaggagcta gcgagttggc agaagtcagt gaagacggat taacttacaa
    14521 aatcaaatta cgagaagacg caaaatggtc aaatggtgat cctgttacag cagcggatta
    14581 cgtttatggc tggcaacgaa cagtagaccc agctacagct tctgaatatg cttacctgta
    14641 tgcgccagtt gaaaatgcag aagacattac agctggaaag aaagataaat cagaactagg
    14701 gatcaaagca gttggtgact atgaattaga aatcaaattg acgaaacaaa caccatattt
    14761 ccaatatctg cttgctttcc catcattttt cccacaaaaa caatccgtag tggaagaaaa
    14821 tggggatgcc tatgcatctg caagtgataa agctgtttat aatggaccat tcacattaga
    14881 agattttgat ggaccaggaa cagatacaga atggacttat aagaaaaatg atcattactg
    14941 ggacaaagat acagtaaaac tttctgaaat caaagtcagt gtagtaaaag aatcttctac
    15001 tgcattgaac ttattcaaag atggccaagc agatgacgtg atcttgtctg gtgagttagc
    15061 acagcaaaac gcaaacgatc cagcttatac ttctgttaaa gaagcgcgga caagctatat
    15121 cgaattcaat caacgtgaaa agaattcacc attcaacaat gtgaacctaa gaaaagctat
    15181 ttcttattct atcgatcgtg aagctttagt aaaacaagta ctaggtgacg gatcagttgt
    15241 ctctacagga ttgatcccat cagatatggt aaaaagtcca acaacgaacg aagactttgc
    15301 aaaagaagct ggaaaattag tttcttatga caaagataaa gcaaaagaat attgggaaaa
    15361 agcgaaaaaa gaattgggcg ttgattcttt agaatttgat ttgatggctt ctgatgatga
    15421 ttcatcgaaa aaagtaatcg aatatgttca aaactcaatc caagaaaact tggatggcgt
    15481 aacagtcaaa ccaacacctg ttccattctc tgtacgtctt gaccgctcaa cttcgggtga
    15541 ttttgacgca gtactaggcg gatgggctgc tgactatgca gacccaagca gctttactga
    15601 tttgtttgtc acaggtaatt catacaaccg tggtcgctgg agcaatgcag aatatgacga
    15661 agcagttaaa gcagcttcat caacagacgc tggcgatgaa caagcccgtt gggatgattt
    15721 ccaaaaagca atcaagatca tctctgatga tatgggtgtc gttccggtgt accaaaaagc
    15781 agaaggtcac ctagtgaatg aaaaaatcaa aggtatcgtt caccatgcgg caggcgcttc
    15841 atgggattac aaatggacat acgttgaaga ataatcgtta ataccgtgag catgagaaaa
    15901 aacgaggctg cggcaaatgt cgcaacctcg ttctcatatt cacttatcct aacaaagaaa
    15961 aaagcatgaa aatagcagaa agagcaatta gtattccggc aatgatcagc caaaagctgt
    16021 acataccgta gctggcagac gagagcgagg aaaattctgg ttttttcttt cggttccttg
    16081 ttcgtgcaag atggaccgat cgctgaaaat gatcgaagaa acccgaagaa aagacccata
    16141 aaaaaccgcc gataatcaga aacggcaatg accataagaa tagatcatta gataattgaa
    16201 tgagtgtaaa ggaatggttg atgagatttt ttattaaaat aaccagaatc agtagaaaag
    16261 ctactgcgta ggggatagat ttttttttca tttgattgct cctttatttt tggttcatta
    16321 tctaataatg tagcgaaaca tctgtcagaa gtcaaaagct agggggaaac attagtgaat
    16381 agttatatca aatatgtatt aaaacgtgtc tttttcatga tcatcacgct ttggttaatt
    16441 gcaacaatca cctttttctt gatgcaattg ctgccgggca cgccatatac gaatcaggaa
    16501 cgtttgagtc ctgaaacaat cgagatgcta aataaacaag tcggtttgga taaaccagtt
    16561 atcgtacaat atgggattta tctttctaat ttgcttcaag gagattttgg aatttccttc
    16621 caatttaaaa accagccagt cgcacatttg ttagctggaa gaatcggacc atccttgcag
    16681 ttagggttac aggcaattat ctttggaaca gtcctaggga cgattttagg aacgatctct
    16741 gccatgaagc aaaatacatg ggcagataca tcttctacac tcgtagcgat attaggtcgt
    16801 tccattccaa actttgtatt tgctgtttta ttacaatata tctttgcaat caaattacaa
    16861 gttttgccaa tcgcaaaatg ggatggcttt gtgtacacaa tcttaccaac aattgcgttg
    16921 gccatgtcgc cgcttgctga ctctgctcgg tttatccgta cggaaatggt ggaagtactg
    16981 catagtgatt atgtggaatt agcaaaagca aaaggattga gccgttggga aatcgccttt
    17041 aaacatggtc ttcgaaatag tttgattcca ttgatgactt tattaggacc gttagctgta
    17101 gcattgatga ccggatcact ggtggtagaa aatatctttg ctattcctgg tatcggggaa
    17161 caattcgtta agtcaatcac aacaaatgat tatcctacga tcatggctgt aacgatcctt
    17221 tactcattta tgctgatttt tgttattttg atcgtggatc tattgtacgg tctagttgat
    17281 ccaagaattc gtgtttcaga agggagccga ggataaatgg aaatgatacc aaagcgttat
    17341 gaaacagttg cagatatccc tgcggatgaa tttcttccat tacaaaaaaa taccgaagaa
    17401 gaacgtgaac agattactgc accttctttg aattttatcc aagattcatg gcgccgttta
    17461 aagaaaaaca aagctgccgt tatttcaatg gttttactat tggtcatcat tttgatctct
    17521 gtgatcacaa tctttgtttc tcctcatgat ccaactgccc aaaacgtggc ttatatcaat
    17581 ttgcctcctc gtattccggg aatcaatatc gatggattga atggaaaagc aatggttggt
    17641 ggtgaacttg ttgataaata tgctcaagca aacgtccctt caaacgtaaa tttcttttta
    17701 ggaacagacg gtttgggacg tgatgtatta agccgcttat ttatgggaat gagaatctcg
    17761 ttgctgatcg catttattgc tgcattacta gatgtaacga ttggtgtagc ttacgggttg
    17821 gtttctggtt tgttaggcgg acgtgtcgat aacgtgatgc aacgtttttt ggaaatctta
    17881 tcaggaattc caaacctcgt tgtcatgatt ttgatgttgg tcgtttttga accaggaatt
    17941 ttttcaatcg tggctgctat ggccatcaca aactggatac caatggcacg tatcgttcgt
    18001 gcacaaacaa tgaaattaaa agatcaagaa tatgttttag ctggattgac attaggtgaa
    18061 tcaagaatga aaattgcatt caaacatatt ttgccgaata tctctagtgt cattattatt
    18121 caaatgatgt ttagtattcc atctgctatt ttcttcgaag cctttttaag ctttatcggt
    18181 ttaggtctta cgcctccgtc agcttcatta ggaacgatgt tgagcgaagg gtacaagaca
    18241 ttcctgtatc tgccttatct attatggatt ccggcagtaa ctttatcggt tatcatgatt
    18301 ggctttaact tacttgctga tggattgcgt gatgcctttg atcctaaaat gaaagagtga
    18361 agacgatgac gaaaatttta gaagtgaaag acttagaaat ctcttttgat acgtatgctg
    18421 gaaaggtacg agcgattcgc ggtgtcaatt ttcatttgaa taaaggagaa acattggcga
    18481 tcgttgggga atctggaagt ggaaaatcag tgacgaccag aagcatcatg cgcttgctgt
    18541 caagcaacgc caatatagat gctggtcaaa tcctctttaa aggtaacgat atcgttggaa
    18601 aatcagaaag agagatgcaa aaaatccgtg gtaaagaaat agcgatgatt ttccaagatc
    18661 caatgacctc tttagatccg acgatgccca ttgggaagca agtcgctgag tcccttagaa
    18721 agcataataa agtttcaaaa aaagagggac aaaaagctgc attagaattg cttaaattag
    18781 ttggaatacc cgaagctgag aaacgtatca acagttatcc gcatcagttc tcaggaggtc
    18841 agcgtcaacg gatcgtgatc gcgattgcac tggtatgtta tccggaaatc ctgattgctg
    18901 atgaacctac aacagcgctt gatgtgacta ttcaagcaca gattttggag ctgctgaaag
    18961 aaattcagac aaaaatcgat acatcaatta tctttatcac tcatgacctt ggtgtagtag
    19021 cgaatgtcgc tgatcgtgtc gctgtcatgt atggaggaag aatcgttgaa gttggaacat
    19081 cggaagaaat tttctacaac ccgcaacatc cctatacttg gggactgctt ggttcaatgc
    19141 cgacattaga tagtgcgaac gatcgattgt atgctattcc tggttctcca ccggatttat
    19201 tgaacccgcc aaaaggagac gcattctatc caagaaatga attcgcgatg aaaatcgatg
    19261 cagaacaaga accaccattt tttgaactat caaaaacaca caaggcagca acttggctac
    19321 ttgctccgca ggcacccaaa gtgacaccgc cagaagaaat ccaacgtcgc tgggctatct
    19381 ttaaagagaa acaaaaacat acttacggtc aaggaggaat tgctgatgcc aaataaacta
    19441 cttgaagtta aaggattgaa acagtatttc aatgtaggaa aaaagaatga agtacatgct
    19501 gtaaatgaca tcagctttca catttatgaa ggagaaacat tcggtttagt tggagaatct
    19561 ggaagcggaa aatccactac tggccgtacg atcattcgct tgaatgaacc tactgatggt
    19621 gagatattat tcgacggtca agatataacg aagatcaaag ataaaaaagg tttgacgaaa
    19681 ttccggcatg atgttcagat gatctttcaa gatccatacg cttcgttgaa tcctcgtatg
    19741 aaagtcagag atatcattgc cgaaggaatt gatgttaatg gactagctaa atcatctgaa
    19801 gagcgtgcaa aaaaagtcga tgatctattg agaactgttg gattgaatcc aagccacggt
    19861 actcgttatc ctcatgaatt ttcaggtggg caacgccagc gtattggtat tgcacgagca
    19921 ttggcagtaa aaccgcgttt tatcatttgt gatgaaccaa tttctgcact agatgtgtcg
    19981 atccaagccc aagtggtcaa tttgttgcaa gatctccaaa aagaacatca attgacctat
    20041 ctatttattg cacatgattt gtcgatggtt aaacatatta gtgaccgaat cggtgtaatg
    20101 cataacggct tgttattaga gatgggaaca agtgatgaaa tctataatca cggtgttcat
    20161 ccttatacag aaagtttgtt atctgctatt cctctaccag atccagacca tgagagaaaa
    20221 cgcagaagaa tcaaatatca acctgaacca gatgacggac aagtacgtca acttcgtgaa
    20281 atcgcaccag aacattttgt ctatgcaact gagcaggaag tcccatatta cgcaaaaaaa
    20341 ctaaaacgac aaaaggaatc tcttctggtt gctaactagg agggacatag atgctaaaag
    20401 cttttaaatt ccgtatgtat ccgacagaag aacaaaaaca gcaattgatt cgtacttttg
    20461 gatgtgctcg atttacctat aaccatttgc tgaaaaagcg gcagaaaagt tggcaacaaa
    20521 caggagtagc caatttttcg ctcacacccg ctacattaaa aaaagaatac ccattcttaa
    20581 aagaagtaga cagtcttgct ttagcaaatg cacagcttaa tttggatcgg gcttttcgca
    20641 attatttcaa gggacgtgcc agcttcccaa agcttaaaac gaaaaaaagt atgtggcaat
    20701 cttatacgac caacaaccaa acccgtacga tctatctgga aaatggctac ttaaagttac
    20761 caaaacaaaa agaactaatc aagataaatc agcatcggcc agttgaagga tcgattcgct
    20821 ccgctacgat ttctgctcga tataatgaag aattttatgt cgcactactt tgtgatgttt
    20881 cgccagtaaa aaaagaatct ttagctaaat ggatcgggat cgcctatcat ccgaagacat
    20941 tgatccaaac atctcgacct ctagaagtaa ctttaccaaa atttcaccag acagaagaaa
    21001 agctgcaaca tgcacagcgg aaattgaatg tgaaagtccg ctccgctcac catagaaaga
    21061 tcaggttaga tcaggcaagc aattatcaga aacaaaagag aaaagtaatg gatctttatc
    21121 tgaaacaaaa gaaccagcga gaagattatc ttgaacaatt atctgggaaa ttggtcaaac
    21181 aatatgacta cttatttgta gaatcttttc caaaagaaga ggcacatgct gatttttcta
    21241 ttcacgactg gcataaatta ataacaaaat tgcgttataa gtcccaatgg tataataaaa
    21301 aatttctttt gatcaatacc gacggagcag aagaaagcaa ttccgtaaga aagagccaag
    21361 tagtagaaaa aatgggcaga cattcggtta tcaagggata aaaaatgaat gaaactgata
    21421 ggagcgttag ggttagcctt gttaataaaa agtcttaatg acttttgttt ctaggaattt
    21481 ttcttttgaa gaaaagctca ctaattatga caaaccaata aaacaatagc agaaaaagtt
    21541 cgtacgagct ttttctgcta ttgttaattt tttattcaga aagtttacga atacatcaaa
    21601 attctgaaac aaaaaacgta actcacaaag agttgttttt gcgttttttc ttgaatgatt
    21661 aggtctgaat cttttgctca caaccttatt catggtgccc cggcttgagc tgatagattt
    21721 ttgaaggacg gccgattcct acaggacgtt caccgatctc atcgaaatat tggagcatag
    21781 ctttcttaaa attggaatga tcgatcgttt tataatcgat ccctaagaat ttagcaaata
    21841 cttttcgagc ttccgtaatc gtgaaatctt tacctaaaac ttgtagaact tgcggctcgt
    21901 gttccatttt attgactaca cggttgaatg ctttcaagat gatttgacta tggtcaaaag
    21961 ccaaagtatc tttgccagaa gactctccag tgactaaatc aagcgagata gccacttctc
    22021 cgctagataa atggatcttg ttactatgac gttctagtgt gaaccagcgt acttccttgg
    22081 catcatctcc ggcgattagc ggttcttctc caataaaggc taagtagctg actgtgacga
    22141 cccagcctcg cggatctcga ttaggcgtac tgaatgtatg cagttgctca atattttctt
    22201 ttgaaataac cacaccagtt tcttcctttg tttctcttaa gacgctttct ccagttgact
    22261 catccttttg gacaaagcca ccaggcaatg cccatgaatt tcgataagga tgcccttttc
    22321 tttggatcaa caaaactttc agctgatctt cttctttgtt atagcacatc agtacgatat
    22381 cgacagtcaa agagggcttg tcatattctg gacgttcctg tttttgatac cagttaagaa
    22441 attcttcttc acttgcttta tgttcataat ataatttttc ttcttcttta gaagcaaaac
    22501 ggttcatctg accacctcca ttgacagtaa aagtactttt taccttattg tagcagactc
    22561 tgaaaaaaaa ccaaggattt acttgaaaaa tctaggttat catttaaata tgaaaaataa
    22621 atggaagtat gagattgaat aaaaagaaaa gcagataaaa cggcaatact ttataccgct
    22681 cttcacgaat ggcaaagaag ccacaaataa cgatatatac gtcaatgata aaacgcaaaa
    22741 tccaagctgc tgtcataaaa ctgttcaaat gcccatggag gttaaaataa ccatacatac
    22801 gaatataaaa atagctgaat attaaatgtg ctaatgaaag aaaagcaata ctaagtgtaa
    22861 aaaaaagatg tttcttctgc atgattgttc cagtccctta ctaagataga tagtctttat
    22921 tataccacgt gtgaggggag aaataaaaag ccgtttttta gtagatatga aacaaaacaa
    22981 aaacccgcat ttattcaacc tattcatcct tttaaaggat agattgaacg tagatagaag
    23041 tcgtttatcc agtctttttc attttgaaaa aaagctaaca gcatgctttt tttatggtat
    23101 actagcaagg aaacgaattc gcatcatgcg ataaaggagg aagagccccg tggcagaaaa
    23161 ggaaactttt tatatcacta cgccaatcta ttatccaagc ggcaaacttc atatcggcaa
    23221 ctcgtatact acgattgcat gtgacgcaat tgctcgttat aaacggttga tgggctttga
    23281 tgtgttttat ctcactggag tggatgaaca tggtcaaaaa atcgaaacaa aagcagaaga
    23341 actaggtgta aaaccgcaag aatatgtaga taaaatggcg gctgatgtca agaaattatg
    23401 gaaaacgctt gatatcagtt atgataaatt cattcgcacg acagatgatt accataaagc
    23461 agctgttcaa aaaatatttg atcgtttatt agaacaggga gatatttact taggtgaata
    23521 tgaaggctgg tactctgttt ctgacgaaga attctttaca gaaacacaac ttgcagaagt
    23581 gtacaaagat gacgaaggta acgtgatcgg tggtaaagcg ccaagtggac acgaagtaga
    23641 attggtaaaa gaagaatcat acttcttccg catgagcaaa tacgcagacc gtctattgga
    23701 atactatgaa gaacatcccg aattcatcca gccagaatct cgtaaaaacg aaatgatcaa
    23761 caactttatc aaaccaggat tagaggatct tgcagtttca cgaacaactt ttacttgggg
    23821 agtaccagtc aagagtgatc cgaaacatgt ggtgtatgta tggatcgatg cattatccaa
    23881 ttatattact gcactaggat atggctccga agatgaaagt ctattcgaaa aatactggcc
    23941 tgcagatgtt cagatggtcg gtaaagaaat cgttcgcttt catacgatct attggccaat
    24001 catgctgatg gcccttgatt tgcctctacc taaaaaagta tttgggcatg gctggctatt
    24061 gatgaaagac ggaaaaatgt ctaaatcaaa aggaaatgtc gtttatcctg aaatgcttgt
    24121 ggaacgttat ggtttagatg cactccgtta ttatttgctt cgtgctgttc cgtttgggtc
    24181 agatggtgtc tttacgccag aagattttgt ttcccgtgtc aattatgatc tggcaaatga
    24241 tctagggaac ttattgaatc gaacgattgc tatgatcaac aaatattgtg atggactcgt
    24301 tcctgactat gcctcacttg tcactccatt tgatagtgaa ctgtctacga cggctgctaa
    24361 cgttgtcgga cgttaccatg atgcaatgga aaaaatggaa ttcaatacag cattggctga
    24421 aatctgggtg ctgatttctc gtgcaaacaa atacattgat gaaacagaac catgggtatt
    24481 agcaaaagac gaagaaaaga aagctgaatt gaatagtgtc atgatccatt tagctgaatc
    24541 tttgcgtatc gcagcgatat tacttcagcc aatcatgaca gaaacgccag agaagatctt
    24601 caatcaacta ggattggact ctgaaacaat gaatcttgaa ggattacact ttggcgaatt
    24661 tccttcagga acaaaagtag ttgcaaaagg tacaccaatc ttcccacgtt tagatatgga
    24721 agaagaagtt gcttttattc aagaaaaaat gtctgaaggt actcaaacaa acgaagatac
    24781 agtcaaatgg gatccagaag aaacagaact tgtttcaacg aaagaaaaac aaatcaaatt
    24841 tgatgtattt gaaaaagtag aactaaaagt agcagaagtc atcaactgtc aaaaagtaga
    24901 aggggcagat aagttgctgc aattccgact agatgctgga gacagccaag accgtcaaat
    24961 cttgtcagga atcgctgaat tttatcctga tccaagcgaa ttgatcggga aaaaagtcgt
    25021 cattgtagca aatctgaaac cacgaaagat gcgtggacaa atcagtcaag gaatgatcct
    25081 ttctgcagaa gcaccagatg gctctttaca agtaatcgaa gcaccaaaat ccatgccaaa
    25141 tggctcagaa atcgcataga aattcaaagg gggtatgaca ctagttgtgt cataccccct
    25201 ttttccgaaa tttgagaagc aattttcgta aattccttct tattacttga agccaatcat
    25261 ataatacaga aacagagaac tatccaaaat aaatcctaga aaaagaagta cgagcttcgc
    25321 tgatttttgt taatttagaa taatactaat aggaatcgta aaaacagaaa aaaagcataa
    25381 tgataaaggg tcgggcagat tttaattgat tcatctggta ctctttttta ttgtccttac
    25441 attcataaag tttgaatgca atgactttat tacatctata tttatatcta acagtagcgt
    25501 tgatgtgtta attataagtt ctatcacttt atgttcaatt agtttgtgct cttatatgtg
    25561 tgattgaaaa tatagataaa aaaacaatca tcggctttga cttcgatgat tgttttcttt
    25621 attttgtaaa aatcctatca cttgatttta tggtaacctt ttatttttgt cttcaaacgg
    25681 cttggtaaga gagcagtaag cagtgcaaat gggatcgaaa agacatacca gaaaatcgta
    25741 cgtccgatac cataatgttg atagagcttt gtcaacatca cgtgtaacaa gatcaaccct
    25801 aaaagcaagg agccagctat aaaccaatga ttatcccaat gcatgacttc actaagtgcc
    25861 atcactccaa aaggatagaa ataaaaatag atcccgacta aggtcatccg ccagttcttt
    25921 gtaaatgttc cgatattcaa taaaaagaaa atcgctgaaa gcaaaatacc gataggcgga
    25981 aaaagcccaa ccaaaaagga atacaacatc ccccacaaga tcatcacagg acctttgatc
    26041 aatgcaacga ttgcgatgaa caaagtcagc aatattagtt tttgtgataa cgttgcaaca
    26101 gtcattaatc caaataaaat taatgtgaac caatgccaat gggactgctg ttgttcttta
    26161 cgggaaaaat cgacccggtt ggataaataa tctgctaatt tcggataaga acgcttaaaa
    26221 tccataaact gttcctcctt cgtggaaaag gtaggtttag tatacgaaaa ataaaaaaga
    26281 aatacatccg tcttaagagg gagtttttca ctgaaagtgg agaaaaaaga atggtaaatt
    26341 aaaatattct cagttatact gaaaattata ccaaatttca aagaagaaag aggatgggaa
    26401 tgaaaggcaa actaaaacga gaaatcaatc tgtttggcgc attagctacc gttatgggaa
    26461 ccgtgatcgg tgcaggcgtc ttcttcaaaa ctgctgctgt tactgctagt actcagtcta
    26521 caagtttgac attgctagct tggcttttgg gtggtttctt aacgatctgt gctggattga
    26581 cagtggctga attagctaca gcaatcccgg aaactggtgg agctgtaaaa tatattgaag
    26641 ctgcgtatgg aaaattgccc agctttttat taggatgggc gcaaagcttg atttactttc
    26701 ctgctaatat tgctgcatta tcgattatct ttgcgacaca attgacgaac ttattacaat
    26761 tatctaccga ttatcttttg ttgattgcag tgatcaccgc tgtttctgtg acagggttga
    26821 atttgttagg gacaaaagtc ggtgcttctg tacaatcagt gacgttgatt gttaaattaa
    26881 taccaattgc tgtgattgtc atatggggat tattgacccc aggacaagga actgtccagt
    26941 tatttccaat cgaagctgga aaagatgtta cttttgtcga aggattgagc agtgcgttgt
    27001 tagcaacgtt gtttgcttat gacggctggt taggtgtcgg tgcaatggca ggggaaatga
    27061 aacggccgga aaaagatttg cctaaagcaa ttattcttgg tttgagtttt gtaacagtcg
    27121 tttatctatt gatcaatttt gtttttttga aaactttgcc gattgatcat cttgccggta
    27181 atttgaatgc agcttctgaa gcatcagacg tcatttttgg aggaatcggt ggaaagctgg
    27241 tcactattgg gatcttgatc tcggtatacg gtgcattgaa cggttataca ctaactggga
    27301 tccgcgtgcc ttatgcgatg gctttagaag acgagctacc tttcagtaaa cagctgacaa
    27361 agctttcaaa gaagtttacg gttccttatg ttcctgcggt tttccagtta gcagtagctt
    27421 gtattatgat gagccttggt tctttcgact ttttaacaga catgttgatt tttgtgatgt
    27481 ggctattcag tttactgatt tttatcggtg tctttatttt aagaaaaaaa gcgccggaat
    27541 tgccacgtcc atataaagta ccactttatc caatcgttcc gattattgcg attctcggtg
    27601 ctattttcat tttaggtatg acgatgatga cgcaaacgaa attagcattg atcggtatag
    27661 gtgttacctt gataggtatc ccagtctatt atcaaaaaaa gaaaaaacgc tcagaagagt
    27721 aaaatatttc aacaacagac agaaaaagta cgaactaggt cctctagatc ttcgtgcttt
    27781 ttctatttca ataaaacaag ttcttctatt ttgatttttg ctacttctgc aagtagtgct
    27841 tatgtgctaa agtaagaatg aaaaggagag ataaacatga tatttgattc tcatacccat
    27901 ttaaatgctg aacagttcaa tgaagatatt ccagaaacga ttcagcgagc acaagaaatg
    27961 ggggtaacga aaatggcggt cgtgggcttc gatacgccaa cgattgaaaa gtctttgcaa
    28021 ttaagccatg actatccaaa tatatacagt attatcggct ggcatccgac agaggctggc
    28081 agttatacga aagaaatcga aaataaatta caagaacaat tgactttacc aaaggttgta
    28141 gcattagggg aaattggctt ggattatcat tggatggaag acccgaaaga agtccaagat
    28201 aaagtatttc gcagacagat tgctatcgca aaagaaatga acctaccaat cagtattcat
    28261 actagagaag cacttgaaga cacgtatcaa atcctaaaag aagaagatgt tcgagatatc
    28321 ggtggaatca tgcatagttt cagtggtgat tatgaatggg caaaacgatt tcttgactta
    28381 gggatgcata tttcatttag cggtgtcgtc actttcaaaa aagcattaga cgtccaagag
    28441 gctgcagcaa atgtaccaat cgaacgatta cttgtggaaa cagatgcccc ttatctggca
    28501 ccagttcctt ttagaggaaa gcgaaatgaa ccaggctata ctcgttatac agtagaaaag
    28561 attgctgaac ttcggaatct tccgacagaa gaagtggctg cccaaacatg gacaaatgcg
    28621 caccgattat ttggtctaag cgaacatgga taagatgaag atccaagaga ttatagtcgt
    28681 tgaaggaaaa gacgatacaa gaaggctgcg ggaagtagtt gatgcagata cgatagaaac
    28741 aatagggtct gcaatcaatg aagagatcct gatgcaaatt gaacatgccc aagagactcg
    28801 tggagtgatc gtttttaccg atcctgattt ttcaggagaa aaaataagaa aaatcattat
    28861 ggaagctgtt cctcaggcaa aacatgcttt tttgccgcgc acacaagcta aaccaaagaa
    28921 aaagggaagc cttggtgtcg agcatgcaag tgatgctgct attttagaag cattgaaaaa
    28981 ggtcgtgacg ccagacattt cccaaacggc tgtttctgaa atcactagac aagacctttt
    29041 agactatggt ttaatagctg gggcacaagc aaaagaaaaa cgagaaaaac taggtgacga
    29101 gttgcgcatc ggttacacga acggcaaaca attggaaaaa cgtttgaaga tgtttcgtat
    29161 tacgccgaaa gaactcgccc aagcaatgaa aatagtggag gaacaacatg agtgaatatc
    29221 gtgaaattgc gacaccctct agaacaaaag agattttatt gaaacatggt ttttcattta
    29281 aaaaaagttt aggacaaaat tttctgactg aaccaaatat tttaaggaaa atcgtagaaa
    29341 ctgcgcaaat agatgaacat acgaacgtga tcgaagttgg accaggaatc ggtgcattga
    29401 cggaacaatt agccaagcat gcaaaacaag tcgtagcatt tgagatcgat gatcgtttga
    29461 tccctgtatt ggctgatacg atgcaaccct atgacaatgt gactattgta catcaagata
    29521 ttttaaagac cgatttgtca acagctgtta gagaaacatt tcatgaggag cttccgttaa
    29581 aagtcgtagc gaatttacct tactatatta cgacaccgat catgatgcat tttcttgaat
    29641 cagatttggt tgtggatgaa ctagtcgtca tgatgcaaaa agaagtagct gatcgtatct
    29701 ctgctgaacc aggaacgaaa gcttatggtt cattatcgat tgctgttcaa tattatatgg
    29761 aagccagttt agcattcatc gtaccaaaaa cagtatttgt ccctcagcca aatgtagatt
    29821 cagcgatttt gaaactgaca agaagagata taccggcagt cgaagtgaca gacgaaaaag
    29881 catttttccg attgacaaaa gcggcatttc agcaaagaag aaaaacgctt tggaataact
    29941 tgcagcattc gtatggcaaa gatgaccaga caaaagcatg gttagcaaaa agcttggaaa
    30001 cagctggaat cgatccaaaa cgtcgtgggg agactttgtc attacaagaa ttcgccgcat
    30061 taagcaatgc gatgtcagaa aatatgcagt aaaaaaagag gttgtgacag aagttgtgaa
    30121 aaagctgtcc tgcatcaagt aataagaaca gccaaacaaa aatagttcct catgtttttc
    30181 gtttgtttgg gcttattacc gcagatgcaa gcttttgaac accgtttaag aaagaggttg
    30241 tgggcctgcc gtttaactct aagtcacaag gaacaatttc aaaaaacagc ttctcatgtt
    30301 tttgagttag ttggtcttat tacttgatgc aagcttttga ccactattat gccgaacaag
    30361 gagatgtgac acaaattttg tcacatctcc tatttttgtt tcttgatcaa aaaattctta
    30421 tacttgatac tcacactcat tttgtatgga tttagaaaag aatatgtgtc ttgacagtta
    30481 ttagtaagtg agttatacta agtcgacatt tatgtgtcaa gacatattag gagagagaaa
    30541 aaatgagaaa aaattcagtt cgaaaactaa cgatttctgc tttattgatt gggatggggg
    30601 tcattatccc aatggtcatg cctaaaatca tgattccacc agcttccttt actttggcgt
    30661 ctcatgtgcc attatttatt gcgatgtttt ttacaccagg agttgcagtg gctgttgcgc
    30721 taggtacgac atttggtttc tttttaacaa cgccggttat catcgcattg cgagcattgt
    30781 cacatcttgt gtttgcgttg attggggctt ggtacttgca aaagcatccg agtatcgttt
    30841 taaaaaatgg acagtttact tttgctaatt ggcgtttcca agggttcaat tttgtgatcg
    30901 gtgtgattca ttctttagca gaaatgctag tagtcagcat cttttacttt attggtaata
    30961 tgcctgaaac atattattct caaggatatt tttatacagt attcatcttg atggggattg
    31021 gtgggcttat ccacagttta gttgattata atattgctta ttttatagca ggaacactca
    31081 gcaaatattt tgatatcccc gtatttacgg cagcaaaaaa agaaaatcgg aaagagtcga
    31141 ttcaagggta cgcgaagaaa acgacatatt agacatgatc tctcaaaaaa aaaactctca
    31201 tctagtaatg gataataaat cctctaagat gagagttttt tgtcctaaaa agagctgcac
    31261 caacatcttc tggcacagct ctaacagggg gcaatttatg gaagtttgat gacttgttct
    31321 gtttctttat caaagaagtg gcctttgctg atgttgaagg caaggtcgac tatttcgcct
    31381 ggtttatgga aatctcttgc atctactttt gagatgaact cagtagaacc agtttttgtg
    31441 taaagcatcg tttcagctcc aagtaattcg gaaacgacaa cttctgattt cactacggct
    31501 tcaggtgaag cttcaagggc tacttgttca ctgtggatgt cttctggacg aataccgaag
    31561 ataacttctt ttccttcata acctttttca actaatactt tgttttttcc ttcaggaata
    31621 cgaagcttaa gcccatgtcc atctgtgata acaccatttt tcaatgtcac attaaagaag
    31681 ttcatggcag gagatccaat aaagccggca acgaacatat tgacgggtgt atcataaact
    31741 tctttcggtg taccgatttg ttggataaat ccgtctttca tgataacgat tcggtcagcc
    31801 atagtcattg cttctgtctg atcgtgagta acatagatgg tcgttgtttc aagacgctgg
    31861 tgaagtttgg caatctcagc acgcatggct acacgtaatt tagcatctaa atttgataat
    31921 ggctcatcca ttaagaagac ttttgcatcc cgaacgattg cccgtcccaa tgccacacgt
    31981 tggcgttgtc caccagataa agcagctggt tttcgtttca agtattctgt cagacctaag
    32041 atctctgctg cattgtccac acgttttttg atgtctgctt tatcatattt acgtaatttc
    32101 aagccaaaag ccatattatc aaaaacagtc atatgaggat acaatgcgta gttttggaaa
    32161 accatggcaa tatcacggtc tttaggtgcc acgtcattca tgacttttcc gccgatattt
    32221 agttctcctt cagagatatc ttccagtccc gcgatcattc gcaaagtagt tgactttcca
    32281 caaccggaag gaccaacaaa aacgataaat tcacggtctt tgattgatag attaaaatca
    32341 gttacagaat aattttctgc attgtcatat tttttatata cgtttttcag agccatttct
    32401 accataatac gttccacact ttctttttta gttttattaa ctatttaaag tataaatgaa
    32461 agcgttaact aacttcaatg tcatgttgaa caagaatttt ttcttttttt tgtcaatgtg
    32521 ctagttttaa gctttaataa atatccataa gaaataaaaa ctgatacact ggagtaaata
    32581 agtatgttaa caatatcaaa aggagtgtct cttatgaaaa tcgctttgat cgctcatgac
    32641 cgaaaaaaac ctatgatggt gaaattagca acagcgtata aaacaatatt ggaaaaacat
    32701 gaactttttg ctaccgggac gactgggcag aggatcacgg aagcaaccgg cttgccgatc
    32761 catcggttta aatccggacc gttaggcggg gatcagcaaa ttggagccat gatttcggag
    32821 gatcagttag atttggtgat ttttcttcga gacccgctgg ctgcacagcc ccatgaacca
    32881 gatgtaacag cacttatccg tttatctgat gtctatgaga ttccgttagc tacgaatatc
    32941 ggaacagctg aggtgctact aagaggactg gaagccggct tcctagattt tcgaagaatc
    33001 gtccatgaaa tagacaagca tccactttca ttttgatgga taagcgtatg gaaaaataac
    33061 gtttaatgtt cgcctatatg tagctataaa agctaaacaa actgataata tgcgatataa
    33121 tacgaacgta ttggataata taaacaataa tattagtttt tcgtatttgc agtaaatgtg
    33181 aaaatatgct atagtttctg tgggaaaaga tcccaaaaat aatacagagt agaaaataaa
    33241 gcgttaagtg ctaagaggat gggatgttgc ctcttggacg aaggacgtaa tgttcgcggt
    33301 tttattttcg cattcgctgc atgatacgat gtagcagctc ttaaaggaga tgctaaaaat
    33361 gaagaaacac cggttagacg cacgtatgat tgctattatg ggcttattga ttgcattgat
    33421 ggtcactttg tcccgactgg tagcaatcga gaccccattt attaaaatca gtgtcacatt
    33481 tattccacaa gtgatcatgg gcattctttt tggtcctttt tggtcaggga taggcgcagt
    33541 tttagctgat ttagtaggaa tggccctctt ctcgaaatct gcattcttta ttggatttac
    33601 attgaatgca tttattgaag gagcaatcta cggattcttt ttctatcgaa aagagatcac
    33661 ttggaaaaat gccattctgg ctacgcttag tgtcacgctg atcatcaact tatttttgac
    33721 accattatgg cttgcgttga tgtaccatgt gccattgttc agctgggtcg tttgggcgcc
    33781 ccgcctgtta aaaacagtaa tatggttacc gattcaatca attgccattt attacgtagg
    33841 acgtagtatt ccttacaaaa aaattttaag aagtttagcg atccacgcta aataaaaaac
    33901 aagcagtctt cccatcccag aagactgctt gtttttattg tgctgtgaat tgtgcttgat
    33961 agagccgtcg gtaataacca tctttttgaa gcagctcatc atgggtgccc atttcgacaa
    34021 tagctccctg atccatcact aatatctggt ccgcattttt gattgtggac aggcgatggg
    34081 caatcacaaa actggttttt ccttccatca ttgctaagaa ggcttgttgg atttttttct
    34141 ctgtcaatgt atcgactgag ctggtagctt catctaagat caacataggg ggattactga
    34201 tcatcgttcg agcaatcgtc aacagttggc gttgtccatc tgatatcttt aacccttgac
    34261 cgccaatctt agtatctaat ttttccggca aacgttctac aaattcatac atataagagg
    34321 cttttagggc atcattgatt tctgcgtcac ttgcttccgg attcccaaaa cgaagatttt
    34381 ctcgcaatgt actgtcaaat agccaagtat cttgtaacac cattccaaaa cttttacgta
    34441 atgtatcacg ttggatagag cggatgtcat ggtcatcgac tgtaattgtt ccttttgttg
    34501 tgtcataaaa acgcatcagc aaattaacaa gtgtggattt tccggcccct gtttttccta
    34561 ctatagcaat ggtttgacct ggataagcag taaaattgaa atcttcgatc aaacgctgct
    34621 tggggtcata agaaaaagaa acatgatcaa aagagacttt tcctttgata tttttgggtt
    34681 ccaaggcata ggcatctggc aaatcaggct tttcttctgg ctgatcgatc aaatcaaatg
    34741 ctcgttctag accagaaaaa gcggtttgca gttgtgtcgt gattccagaa agttcgataa
    34801 atggtttaga aaactgactt gagtagatcg taaagctgga aatcacacca acagtgattg
    34861 acgaaccact cttcaatgcc aataggccgc caactaaacc aatcgataaa taagcaaggt
    34921 gatctacaaa acgagaagac gggtttgtca aggaagaaga aaattgtgcc cgttgtcctt
    34981 tttcatatag atcagcattt agcagttcaa attgctgttg actagcttct tcttgctgaa
    35041 aagctttcac gattttctgg ttaccgattc gttcggtcac aaagcccgag atatccccaa
    35101 taatcctttg ctgtctatcg aaatttgttt gtgaagcacg agcaacgatc caactcacaa
    35161 ggaaaataat tggtgtagaa caaagaacga ccaatgtcag aagtggactt agacgcatca
    35221 taaaaataaa cgaaagaatg atgattgcca ctccagaaaa taattgattg aaggccgcag
    35281 aaattgcaat ggaaatattg tccatatcat ttgtaaatcg actgacgata tttccgtggg
    35341 agttttgatc ataataacga agtggcaatc gattcagatg agcaaatgca tcttttctta
    35401 attttgcaac ggataagtaa gctaaccgat tgcttaacac ttggatgagc cattgactga
    35461 ctacgttgat taaaacgata ccaccgaata gaataagtaa atggaacagt cgattgaagc
    35521 ggacctgtcc ttctccaatc atttgatcga tactttgacc aatttgtaac gtcatcacaa
    35581 ctgttgcaat cccattgatg attcctagta tcacagctag ccaaatgtct tttgcatagg
    35641 cagtaaggta aggaatgaat cggcggagcg tctgcaagga taattttttt actttcataa
    35701 ctattcttcc tcctgtgatg cgacgatttc ctgatattct gctgattgag caagtaattc
    35761 ttcatgggta ccctgagcag caatcttacc ttgatccatc actaagatat gatgtgcttc
    35821 ttgaatagag cggacacgtt gggagataat aatcactgta ctgccaagat aagtctgtag
    35881 cgccttacgc aaatccaagt ctgtttgata atccaacgca cttaaagagt catctaaaat
    35941 cagtaatgct ggcttggcaa tcaaagctct tgcaatcgtc agtcgctgtt tttgtccgcc
    36001 agagaaattt ttcccatttt ccatgacttt tgtatctaaa ccatttggaa gctgacggac
    36061 aaattcttct gcctgagcga ttttcagagc ttcccaacat tctttttctg tggcgtcttt
    36121 cttaccccat tgaaggtttt cacgaatagt cccggtaaat agtacagaag tttggggaac
    36181 aagagcaatt tgctgacgga gatgatccag ctgccaagaa cgaacatcca ttccgtccat
    36241 tataactgta ccggaactga catcatagaa acgcggaatc aattgtgtca aagtcgtttt
    36301 cccgcttccg gttggaccaa tgattccaag gactgtattt cgcttcagct gaaatgtaat
    36361 attttggaga gaaagaccag aatcaggagt ataacggaag tcaacatggt caaaagcgat
    36421 aattgcttca gaagaaaaat cgggagtagt actgacctca gcttcaagaa tcgaattttc
    36481 tgtgtctagc acctctttca cccgatttcc tgaagcttcg gcacgtgtga agatcacgac
    36541 gaggtttgaa acaacgatca gtgccaaaag catttgattc atataattga tcaaagcaag
    36601 aacttgtcct tgttgcaagc taccaatatt tactttgatc ccgccaactg tcagcaaaca
    36661 gatgatcccg acattcatga tcaaggttgt agcaggtgat aatagggcag agatattgga
    36721 aacacgtaaa taattgttcg ctagatcatt tgtgctttta tcgaacttct ctgtttcttt
    36781 ttttgtctgc gcaaatgcgc ggatgacacg aacaccgcta agattctgac taacgtgttc
    36841 attgagctga tctagttttt cttgcacttt ttggtagaga ggaacggttt ttttgatgat
    36901 aaaaaataaa atgattgaga aaataggcaa taatgctaag aaaaacagac ctacttccca
    36961 gtcgatcaca aaagccatga ccactgaccc gatacttaaa aacggtgctc tgatcaataa
    37021 acgaatgacc attgctaaag ctagctgaag ctgattgatg tcgtttgtca ttcgggtgat
    37081 caaggtatct gttccaaaat gattcaactc gttgtgagag aaagtattga tttttttgat
    37141 tagctgatta cgtaactctg taccaaatcc ttgtgaagct actgaagcat agtattggca
    37201 aatgatagca caaatcagac caatcacaga catagcgacc atccataagc ccatttcaat
    37261 gatttttcct cggtcattca tttttagtcc attatcgatc aaggaagcca tcattaaagg
    37321 caggacaagt tcgaagacag cttcaagaaa tttgaagaac gggcccaaaa tgatttgctt
    37381 acgatagtct tttgcataac gtaataactg aatcatatga ttcctcctat tatttatttg
    37441 tccacaattg aaaaaacaag tactaccaca ttgtagctta ggaaatttag aatgaaaaga
    37501 gaacaagcct tttcataaat gaaagtttta ttatgaagaa gctttagaaa ttgaatagac
    37561 tatttttttt aaaaaaaaga aaatgagaaa acatgccagt ctcttcattc ataggaacgt
    37621 ctattttact taatttttct ataaatgaat tctgcataaa aaatatatca agtaaatgtt
    37681 tatagatgaa actctattta cttctttttt aatttgttct tgcaatcttt tttgctacgt
    37741 aatttttgta aaaaagtggg ttgagagaaa tctaattgat tttcaaagaa ggagtatgaa
    37801 aatgagaaaa atatttattc gtttattgtg ggtactatct tagaggaata aataatagtg
    37861 gagaaaacta tggtttggcg cagattctat caggtttgat ggaacgaaga caacctcccg
    37921 acatcaagtc aaaagctact tgagattttg atctgaaaat aaaaaaagaa aacaaggtag
    37981 gaacatcaat gtttctacct tgttttcgta atcaatggga aaaaactaag agtggtgtac
    38041 gtaatcaata aaaaggatgg tgacaacagt acctctttac tctctgaaag catcataact
    38101 cgtttttcct cattatgcca ataaattgtt gttataccac atataaggat ttgtaatagc
    38161 taaaaacaga caggaaagtt gaaatctgtg aatcttattt tgtatggaag gatttataga
    38221 acgcaaaaat caagataaag atgaataaaa atcctgtttc ggctatatat tgcgtatcta
    38281 cgccaataaa acgagtttgt tctgtcatag ccatataaag caaaggcaaa aatacaacta
    38341 cagcgagata agatccgatg atttctcgaa aagttttcca ggaagtatgt ttcctgtttt
    38401 tatatttatc cgatttcatt gaatagtcat agcctcctta ttctttcata tgttgaaatt
    38461 ccagtataaa aactggaagg aactgtacgt tttttagaat aaaaattata agggataagt
    38521 aggctgctgt ctataataag atatattttt caattcttta ttaaagattt tattgacaaa
    38581 atgaatccgt tttctatgat ggaggagggg tgaataagtt gttgaagaag gaaattttat
    38641 gttacttaga gaaccagaca gcctttttcg atttagaaca tatgaacgag gtctttacag
    38701 cacaaaggtt agctgatcat tttggggtaa aaagaaacac agtcagtcat tatttgaacc
    38761 agttaaatga tgagcaacta ctagtcaaaa tcaattctcg accagtgatt tatttccaca
    38821 aagaagcttt tgaaaaacaa ttttttaagt tacgcacgaa tttttatttt tctatcgatg
    38881 atctcaaaga ggaacagcct tttttcgcgc aaccaaaaga cttattctct tttatgatcg
    38941 gatacgacgc cagtttgaaa gaaagtatcg aacaaatcaa aacagctctt tactatccag
    39001 atggcggatt accacttttg attacaggcg aaagtggcac aggtaaaagt tatttagtta
    39061 atttggtcta tcagtattgt ctgcttcatg atctgctaga agattctgca ccattcatca
    39121 cttttaattg tgcacaatat gcagataatc ctgaactttt gactagcaat ttgtttggac
    39181 atgttaaggg agcgtacact ggtgcagaag aaaataaaaa aggggctttt gaagcagcag
    39241 atggtgggat cttgttttta gacgaggtac atcgcttaac accagaaggt caggaaaaac
    39301 tgttcaccta tcttgatcaa ggtgtgattt atcgtatggg agaacctaat ttgtcacgcc
    39361 ggatcaaaac acgtctcttt tttgctacga ctgaagagat tactagtcat ttcttaacaa
    39421 ctttcattcg aagaatacct atccgaatcg aactacctgc acttaatcag cgtagtcgta
    39481 atgaacggct agaactgatt tactcttttt ttctcgaaga acaaagaaaa atggggagcc
    39541 ctttgtcagt cagtggacca gctttgtcac ttttaacaag tcagaaatta tccggaaaca
    39601 taggagagct gaaaaatatc gtcaaagttt cagcagcaaa agcatatgct gaacagcgtg
    39661 aacagcctga aatccatgtc acgatccatc atttgcaaaa agaactactt gcgcagccta
    39721 ttactaaaaa tacagcacag caaggaatct atattacgca agaccagacc ttagagcaat
    39781 taatggagaa gcaacagccc gaacagcagc gaatcgttaa aagttttgag caaatcctaa
    39841 ttgatttcaa gaaaaataac tgtctgctat attcttgcga aggaaaatta aagcaagaag
    39901 tgattcaatt atttgatttt ttgctgtttg agaccagtag acaagaaaaa cacgagctgc
    39961 tggtttattg gacacaatct atccgagaga cctttcgaca aatggaaact gcctatcaaa
    40021 tcaaattcga tggaaacagt gtctatgcct tgagttacta tctctatcag cgaagcacta
    40081 tcaaatggtt accggaagaa catgatatta tccaactaat caaagaatta gaacaacaag
    40141 tttctcagtc ttatccatct agttatcact atgtccaacg aattctggaa ttaagtaaga
    40201 tcaaattaga tattgaagac acaagtatgg atcggatttt actgacgatc tatctaaaaa
    40261 aagcgaaatg gtcaaaagaa aaacgactgc caaaagcgat gattgctgct cacggttatg
    40321 caactgctag cagtatggca aatgtagtaa atcgtttatt acaagaggat ctatttgaat
    40381 catttgatat gccactagat gtcacaccac agcagattgc ttcagaaatc cttgattatt
    40441 gcgaaaacag cgatgtttca aatggattga tcatattagt agacatggga tctctgaaag
    40501 aaatccatca gttttttaaa aaacagttat ccgtaccgct tttgattttg aataatgtga
    40561 cgacaccttt agcaatcaca gtaggggaat gcctgcaaaa aaatgtatca ttagaagaga
    40621 tagcagaaga gactgttcga cagatccagc cagaatggcg actgctctat ccagaggaaa
    40681 acaaacccaa agcactcatt actacttgct tcactggaat cggtacggcg gtccacttaa
    40741 gtgaactgct agaaaaaagc ttacctacgg cttgtcagtt gaaaatcatc ccatatgaat
    40801 atcagcaatt aaaagataag aaaaacagtg acccattatt ttccatatac gaagtcgtag
    40861 gaatgattgg caccacagat cctgatatca ccaatatcga ctatctttca ttagaagacc
    40921 tcatctcggg ggaaaagatg tctgtactag ctgaatggtt ggacactact atgaatgctt
    40981 cagagaagga aatattcaat cagcgcatca ttcgtaattt ttcattggaa aaagtactgg
    41041 attccgtgac tattctagat acagagaagg tgatgggaga aatcgatcat tttatgagag
    41101 aattagaggt gcagttgaat cgaagcatca gcaatcccaa aaaattagcg ttgtatgtcc
    41161 atgtgagctg tctcattgaa cgattgatcc gacaaatgcc aatcgaaacc tatcaaggat
    41221 tagagaaatg gaagcagtgt caaaaagaag ggttatctgc tattaaatct gcgtttagtg
    41281 tcattgaaga gcattatagt gtcatgatcc ccaattctga gattgcctat atctatgata
    41341 ttttgagtga agatacagag tttctctcga tagaagaaga attttgaaca taagcaagtt
    41401 gttgccacta attttggcat gcaacttgct tttttagtat gtgtaagtga aaagaggaga
    41461 tgaaaaagtg gatagagaaa ttatcttagc ttcccatgga aaatttgctt cagggatatt
    41521 agattcatta gagctgatct atgggaaaaa tcattctatt actgtattcg attgctatac
    41581 tgatgagaat tttgatttag cggaaacagt aaggcagctt tttatgaggt acaaagataa
    41641 agaaatcatt gttctgacgg atctttttgg cggaagtgtc aacaacgaat tcttacaata
    41701 tatcaatcgg gatcatgtct atctcgttgc cggattgaat ttgccactat tgattgagct
    41761 agtatctcgg ttggacgctg aagtagagac aactgttttg atccagcagg tgctaactga
    41821 ttcaaaaaaa atgatccagt tttgtaacga gcggctcaat cagaaaatag aagaagacga
    41881 attttaggag gaagaaaaat gattgtattg acaagagtcg accatcggtt attacacgga
    41941 caagtagctt tttcatggac acaaacaata ggcgcagact gtattttgat tgctaatgat
    42001 gatgtaccta cgaatgagat acgaaagaca acgatcaaat tagccaagcc acaaggagtc
    42061 aaactagtga tcaaatcaat tgatgactcg attgccgcgt tgaaaagcgg ggtgacagat
    42121 aaatataagt tattcatcgt agtggaatcg atcgaggatg catataagct ggcagaagcg
    42181 tatccagaaa tcaaaaccat caacttagga gggatcaagg caaaacctgg tactcgcagc
    42241 atcggaaaag cggtaaatat tttaccagaa gaggaggaac gcttgaaacg attaattgac
    42301 aaacaaatag aagtagaaat ccgtcaagta ccagcagata aaaaagtgga tgtaagagaa
    42361 gttttgtaag aaaggaagaa cctcaatgat tgtacaagca gtcttattag gaattgttgc
    42421 atttgtcgca caatcagaat atgcactagg gacatcgttg ctttctcgtc cgattgtgac
    42481 ggggctgttt acaggaatcg tgttaggtga tgtaaaaact gggatcatta tgggcgcaac
    42541 tttagagtta gcatttatcg gatcgttctc agtaggtgct tcgattccac ctgatgttgt
    42601 gaccggtggg attttaggga cggcttttgc tattacagct ggagccggta cagaaaccgc
    42661 gcttttacta ggtttgccga ttgctacgtt gacattgata ttgaaaaata tttacttagg
    42721 gcttttgatt ccaattatga accataaagc agacaattat gcagaagaag gaaattataa
    42781 aggagtcgag catatgcact tgcttgccgg tatcggtctt tccttgatgc ttggtgttgt
    42841 ggtaatggtt tcttatatgg tgggaagcaa tacgatcgga aatctattaa atatgattcc
    42901 tgacttcgtt caaaaggggt tgtcagtagc tacagggttg attccagctc ttggttttgc
    42961 gatgcttgct cgtttgctga tcaacaaaca agttgcacca tattttttct taggttttgc
    43021 tatagcttct tacttagaga ttcctgtgac cggagtagct atttttggtg cgattttagc
    43081 agttgtggta gtcaatatta tgaatgtccg tcaactacca cttcaaacaa ctgcagggga
    43141 ggtagaagaa gatgaagaat tctgagatca aaaacagcaa aataacgaaa aaagagctaa
    43201 atcacgtatt ttggcgttct tttcaaatgg agttttcttg gaattatgaa cgtcagatga
    43261 atatggctta tgtttatgca atgattccga ttttgaaaaa gttatatcag tcgaaagaag
    43321 aaatggcagc agctttgaaa cggcatctag agttttttaa cacgacccca catatcgtta
    43381 cactgatttt aggaatcacg gctgcaatgg aggaagaaaa taaggatgat cctgaatttg
    43441 atgtgactgc gatcgatagt atcaaaacat ctttgatggg acctcttgca gggttaggag
    43501 attctttctt ttggggaact ttgcgattga ttgccacagg tgtgggtacc tctttagcca
    43561 tgcaaggaaa tattttaggc cctatcttgt tcattttgat tttcaatatt ccgcacattc
    43621 tgtttcgcta tctagcaaca ggttggggat atcgcttagg aacgggtttt ttgaaaaaga
    43681 tccaggcaaa cggtatgatg gaaagtttga cattaggcgc gtctattatt ggactgatgg
    43741 tcgttggagc aatgactgcc acgatgatca ccatcaatat tcctttaaaa atcggttcag
    43801 gagaaaatgc tgtgactgtt caaagtattt ttgatgatat cgttccaaat atcttatgtc
    43861 ttggagcatt tggcatagtg ttttatctat tgaagaaaga agtgaaacca ttaacgatct
    43921 tgatcggact agctttcttt ggaatttttg gttcgtggat agggattttt taaaaaagga
    43981 gcagagaaaa tgtttacgat gcaagattat atctatgaag aaaaggaagt actaagtacg
    44041 atcttgaaaa agaatgattt ttcgacaaga gaacatatga aaaaaacagt gaatttactg
    44101 atcttagcaa cgggttcatc ttataacgct tgtttagcag cgaagccagc attggaatct
    44161 tatggtgatc ttactgtcga tattcaagaa cctttttatt ttttcaatta tggaaaactt
    44221 tctccttcaa tcgatactgt gattgctgtg tcacaaagcg ggaaaagtgc ttctactgtt
    44281 gaagcagtaa aaatgataca aaaacaaggg ttacctgttg ttgctatcac aaatgacgtg
    44341 caaagtccac ttgctttaga agctgcacaa attattgatt taggtgccgg ggtagaatca
    44401 gtaggcttcg ttacaaaagg ctattctgcg actgttttac agttactact cttagggata
    44461 ggaattggaa taagtaaaaa taaaatcagc aaaaaaatag aacaagacta catgcaacag
    44521 ttgcgcaaaa taatcaacca tttaccagcg attattcaaa aaacggaaga attttttgat
    44581 ggatatcaaa gtttatttcg tttagcacag cgtttcgtca ccattggata tggtccaaac
    44641 tggggaacag ctaaagaagc agagacgaaa ttgactgaaa caatccgcgt tccgtcacaa
    44701 ggatttgagt tagaagctta tatgcacggc ccatacctgg aggcagatgc ttcgcatttg
    44761 ctattcttta ttgaagggga aagtgtaaat aaggaacgtt cgcaaaagtt gcaaagatat
    44821 atgagtcggt acgttggaga gactctcacc attaccacaa aaaaagcaag aaacgagaaa
    44881 acacttggat tagcaataga atgtgatgaa tatctgtctg tattagcgct tgtcgttccg
    44941 tttcaactat tcgcttataa aaccgcagta gctaaaggta ttgacttgaa caaaaagatt
    45001 tttgaagact ttgatacagt cttgaaaagt aaattataag aggtggaaat catgctgaaa
    45061 ttcaatgaag aagaacaaat caaagcaata aaaggcgcac tagctttacg tccgcaagta
    45121 gaagaaatca tcgataaact ttatgcggaa aaatttgacg ctgtctatta tctaggaatc
    45181 ggtggaacgt atgcttcctc gatgcaagct gtcacttata tgaatgggaa aagcaacttg
    45241 cctgtctttg tacagcatgc tgcagaatat tacactactg gaaacaaaag attaacaaaa
    45301 gattctatcg ttattttgtc ctctgttacc ggtacaactc aagaagtagt gaaagcagta
    45361 gaacaaatca agaaagtagg agcgacactg attggattta tcgataaagc gaacagtaag
    45421 ttgtctcagc tatgcgattt tgtggtcact tatccagctc ctggaacaga acaaatcaaa
    45481 ttttttatgg cagcagatcg attgatgtat ttgaatggag aattcgaagc ttattcagaa
    45541 tactatgaac agctcgaacg ttatttacca acagggttag tggaagcaga gaaaaaagca
    45601 gatgcatttg gcttatcatt tgcagaaaaa catcgacgtg atagtatgca ttattttatt
    45661 ggagcaggga atcaatgggg agctgtttat tcatatgcca tgtgttactg ggaagaacaa
    45721 agctggcttc catcgaaatc tatccatgct gccgagtttc ttcacgggac gttagagatc
    45781 gtagaagaaa caaccccagt cacattattt ttaggcgaag atgaacagag agtgttatct
    45841 gaacgtgtgg ctaaactttt gccaaaaatt tgcagcaatt atacattgat agacacaaaa
    45901 gactatccag tagaaggtat cagtgaaaaa tatcgcggac gtgttttgtc tttcttattg
    45961 atgcatgtag tgacacaacg catcgatgct catgtggagc agttgaactg ccatccatta
    46021 gagatacgcc gctactatcg tcagtttgat tattaggata gactggataa aaagaatata
    46081 aaaaataaag agagcagcga taactgctcc ctttattttt ttaaaatatt ggcatcaatt
    46141 acgttataaa acacattttt tgcattatct acagttcaac ttataatctg tttaatggga
    46201 catgtgggtg ccaaccattt tttgttatgc aatagtacta tttagttgtt gcatggatcc
    46261 gtagtaaaag tccaagtaca acgagttgcg ccagtttaca tagcgttttg tatccagaat
    46321 gttgactaaa acaaaagttc ctatgtcaac cgtcaacaga gacaatctgt ccgttgcagg
    46381 acctgacaga tttagaaatg ttcaatctgt ctcttatgta ttttaaatca atactcacta
    46441 ggagaagttt cctaacctcc taatagtaat gctggtagta acattgtcaa aactgcttcc
    46501 tcttaggaac aagcaaagca taacgagtgt aaaaataaaa tgtagactat tgtaacatat
    46561 cattgattat ttcaagtaaa aacgataaaa gaagtaaaca aacacttaaa aacaatggaa
    46621 attagtccca actttaagaa atgtatacta taatggaaat tatcaattat ttatttggag
    46681 gaatatccat gaaacaaaaa atcgcagttc ttggtcctgg atcttggggc actgctttag
    46741 cacaaacatt ggcagaaaac gggcatgatg tccgtatttg gggaaatgta cctgaacaga
    46801 ttgatgaaat caataagtat catacaaatc agcatttttt acctgatttg acgatcccag
    46861 aatcgattat cggatataaa gaattggcag aagcagtgga taacgcagat gctattttat
    46921 ttgttgttcc aacaaaagcg attcgttcag tcgcacagga acttattggt aaaatgaata
    46981 ctaagccaat cattatacat gcaagtaaag gactagaaca agatacgcat aaacgaatct
    47041 cagaagtttt ggctgaagaa attccagaag aaaaaagaca ggcaattgtt gtattatctg
    47101 gtcctagcca tgctgaggaa gtggcagtac atgatatcac aacaattaca gctgcaagta
    47161 ttgatcaaaa agcagcagcc tatgtacaaa aactgttcat gaatgattat ttcaggattt
    47221 atacaaataa tgatgtgatt ggcgtagaaa ctggtgcagc actgaaaaat atcattgcaa
    47281 ttggtgccgg cgctatccat ggattaggat tcggagatga tgcaaaagca gcaatcatga
    47341 ctcgaggatt ggcagaaatc agccgtcttg gtgtcgcgat gggtgccaat cctctgacat
    47401 ttatcggttt gagtggtgtg ggggacttga tcgttacttg tacaagtgtt cattctcgca
    47461 attggcgtgc cggtaacttg ttaggacagg gacataagct ggaagaagtt ctagaaaaca
    47521 tgggaatggt cgttgagggt gtggcaacga caaaagcagc agtagagcta gctcaacagc
    47581 taggtgttga gatgccaatt actcaaacaa tctataatgt tttgtataat ggagaagata
    47641 tcaaaaaagc agcaaaagaa atcatgttac gtgacggaaa aatggagaat gaattttctc
    47701 ttcgttaaat agatgatcaa tagttaatcg t
//
LOCUS       DAZABD010000016        41353 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000016 DAZABD010000000
VERSION     DAZABD010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41353
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.016"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            354..674
                     /locus_tag="ACHALG_000961"
     CDS             354..674
                     /locus_tag="ACHALG_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HFN2586388.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGLLLISIIGIKI
                     TSGH"
     gene            835..1740
                     /locus_tag="ACHALG_000962"
     CDS             835..1740
                     /locus_tag="ACHALG_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN2586389.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSEGTIRIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVKQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDQAARTILTDAGYPLEDPDKIKLLKKNEELLGTVIQLLSAENVHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            1737..2510
                     /locus_tag="ACHALG_000963"
     CDS             1737..2510
                     /locus_tag="ACHALG_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN2586390.1"
                     /translation="MTLLIKQELFKLIKKKSTAVLSVLLVALLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMESQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSIYLYLLAIIVTVLMKLILFPSISFTEKISTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIALFLALGYLVFKRKNV"
     gene            complement(2687..4111)
                     /locus_tag="ACHALG_000964"
     CDS             complement(2687..4111)
                     /locus_tag="ACHALG_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFN2586391.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNVASIVMSTIFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFINNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGFLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            4322..4768
                     /gene="nrdI"
                     /locus_tag="ACHALG_000965"
     CDS             4322..4768
                     /gene="nrdI"
                     /locus_tag="ACHALG_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFN2586392.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEYEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQRFGFPFLADYELRGTPSDVTRVYQILAEKNK"
     gene            complement(4838..6819)
                     /locus_tag="ACHALG_000966"
                     /pseudo
     CDS             complement(4838..6819)
                     /locus_tag="ACHALG_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
     gene            complement(6833..7729)
                     /gene="murQ"
                     /locus_tag="ACHALG_000967"
     CDS             complement(6833..7729)
                     /gene="murQ"
                     /locus_tag="ACHALG_000967"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFN2586393.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELPSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISEHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEAAQAKLAEANGFVKGTL"
     gene            complement(7732..8793)
                     /locus_tag="ACHALG_000968"
     CDS             complement(7732..8793)
                     /locus_tag="ACHALG_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFN2586394.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQNRLIELGSEAKKNKLELMVDISGEALKRAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPNDIDELKEAAADFSQIEAWHNYYPRLETGLDKEWYQEK
                     NQWLKTQGFLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSEHVQEQFASFQKEQTVLLHTEPVDEVFYEYILGKHTNRQDDARDVIRSADARF
                     REIPPIPVRNTAKRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(9005..9532)
                     /locus_tag="ACHALG_000969"
     CDS             complement(9005..9532)
                     /locus_tag="ACHALG_000969"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFN2586395.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQALAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVVDRLNLPMTADERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECMEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELISYE"
     gene            complement(9599..11101)
                     /gene="eat(A)"
                     /locus_tag="ACHALG_000970"
     CDS             complement(9599..11101)
                     /gene="eat(A)"
                     /locus_tag="ACHALG_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFN2586396.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQAFVYFPQQTKDKERLTYYVLNEIMDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEIAKNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKKVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDALTMNSQVSHHKRLLSVENLQLGYENLLFEPVHF
                     TIEPHQRMAISGPNGAGKSSIIHYLLGTFNGKVVGEKSQPKHLTISYVRQNYEDNRGT
                     LAEFAKKNRVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYI
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLEKI"
     gene            complement(11359..12480)
                     /locus_tag="ACHALG_000971"
     CDS             complement(11359..12480)
                     /locus_tag="ACHALG_000971"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFN2586397.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALGGTWRTIDGMDLLFRQRKISYLLTNWQGKNTAVIESAQVLGLDLLDKP
                     TDQTIRLASSYALGELIKDTLLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTRKLHLDRRNVLVSKVKVIIAADVTNPYTGQQGAAWQFGRQKGGTDTTLAFLD
                     KQAEKIAQQIKDVWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFELVSQITGLEKQI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDRELEGLFDFLPIV
                     LSIQLGPIDLEEAMEHRQTLEKTEILGQTLSRFFNILPK"
     gene            12606..13547
                     /locus_tag="ACHALG_000972"
     CDS             12606..13547
                     /locus_tag="ACHALG_000972"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFN2586398.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVVLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNKHVDLIVALTKANQLDDMFRSIQSIITENTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEAKLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAEGM
                     VRTIVSEFASIAAKEGITLDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAVWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(13690..14586)
                     /locus_tag="ACHALG_000973"
     CDS             complement(13690..14586)
                     /locus_tag="ACHALG_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFN2586399.1"
                     /translation="MNKNDVLLIAKNKSLYFFPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMDFSSSEPKTDLFFGATNNGRKERAEKYKQEFTKR
                     GVTTNYFILPLRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYQFYHSENIFLLQERDLDELPEFLHTPFQPIPSELLEFFDAENWAKR
                     FFTTDSAIYEQYEYDLSSVKNK"
     gene            14868..16208
                     /locus_tag="ACHALG_000974"
     CDS             14868..16208
                     /locus_tag="ACHALG_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFN2586400.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGIILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHAADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFAAYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLIIFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLTLRKGTLW
                     DKGISFFALAVLWLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            16403..16846
                     /locus_tag="ACHALG_000975"
     CDS             16403..16846
                     /locus_tag="ACHALG_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFN2586401.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINAMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILTSKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(17108..18526)
                     /gene="nhaC"
                     /locus_tag="ACHALG_000976"
     CDS             complement(17108..18526)
                     /gene="nhaC"
                     /locus_tag="ACHALG_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFN2586402.1"
                     /translation="MEKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKKYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDILEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPKLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(18930..20153)
                     /locus_tag="ACHALG_000977"
     CDS             complement(18930..20153)
                     /locus_tag="ACHALG_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFN2586403.1"
                     /translation="MTTTLSTNQLKDLIQYRLPEYQALALAIHDHPEVSNYEFYSSAA
                     LIKQLEKEGFWVEKEVAGHRTGFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDEIGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDAALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAALSTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSEKGLASIAIAAELLANTALDLLREPKLLEKIKQE
                     HQEIVARKKQEGATF"
     gene            complement(20185..21024)
                     /locus_tag="ACHALG_000978"
     CDS             complement(20185..21024)
                     /locus_tag="ACHALG_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFN2586404.1"
                     /translation="MKKIIRFAVITFVAIIWLAGCSQGKAATTEKKETTVKLGIIGED
                     TDVWDDVKDRLKKENIHLEYVKFTDYNQPNAALADGSIDLNSFQHQFFLDNYNEEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENKKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDEHARPYVNIIVARKEDEDNKTYQKIVDAYQTEETAKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(21041..21721)
                     /locus_tag="ACHALG_000979"
     CDS             complement(21041..21721)
                     /locus_tag="ACHALG_000979"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFN2586405.1"
                     /translation="MTEFFKTYLPNVYLIPDEFIEATKQTLYMSFWTAFIGGIIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILVLVFLSQFISN
                     QLIKKVTH"
     gene            complement(21723..22796)
                     /locus_tag="ACHALG_000980"
     CDS             complement(21723..22796)
                     /locus_tag="ACHALG_000980"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFN2586406.1"
                     /translation="MIELRDISVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEAGK
                     IIEKGSSIQIFSHPEEELTKDFIRTATHLDQALETISAHAAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRQKALDYLIKS
                     GVKVTILQKNELDNRIKVIEGGN"
     misc_binding    complement(22855..23093)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(23136..23369)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(23446..24117)
                     /locus_tag="ACHALG_000981"
     CDS             complement(23446..24117)
                     /locus_tag="ACHALG_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFN2586407.1"
                     /translation="MKGAIFDLDGLLVDTERTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVQAAGSPEKVQEVRAKREEYFYQQLTSGAIQLKPYAKEILTDLKERHM
                     KLGIATTTVTKRATDILDHFQLADYFDTFTFGDEVSENKPSPVPYLTALERTGLEALS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYSVEEKEKLNLLAEGKDLRTVIEMLD
                     KKTEK"
     gene            complement(24205..26853)
                     /gene="mgtA"
                     /locus_tag="ACHALG_000982"
     CDS             complement(24205..26853)
                     /gene="mgtA"
                     /locus_tag="ACHALG_000982"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFN2586408.1"
                     /translation="MEKKKDTQKIYAAFAGKTKQELFELFHASPNGLSEEQIERSRET
                     YGENAISYGKKTPFIIEILKAYITPFTLVLIALGIISFITEYVLAAPGDKDLFGVAII
                     FTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGEYTEIPMEEIVCGDLVRLS
                     AGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSYENLVFMGSNV
                     ISGSAEGIIVSVGNDTLFGHVAETLSEKPIQSSFELGIHKTSMLLIKFMSLMAPAVIV
                     INGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGTVIKNLNAIQN
                     FGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQTGLKNLLDVAI
                     IDEAAETLDTDTINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGAIEEMLNISKF
                     IDLGGTVAPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFSVKDESDMVLI
                     GYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLEINELITGEKI
                     SEMNDHELAQVAEEHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGINDAPAMKVADI
                     GISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKATASSNFGNMFS
                     VLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKWNASSIGKFMV
                     YFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQQVIFIALFHAGWFVESLWTQT
                     LVLHALRTPKIPFIQSNATFAMLVITTLGIIVGSVLPFTGFGAELGLMPLPGNYWLWL
                     FITVVAYLALVTAVKKIYIRRFQELL"
     regulatory      complement(27196..27370)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            27543..27980
                     /gene="ndk"
                     /locus_tag="ACHALG_000983"
     CDS             27543..27980
                     /gene="ndk"
                     /locus_tag="ACHALG_000983"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFN2586409.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFATMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKGADAVPGTIR
                     GDYALPGTENIIHASDSRDAATKEIARFFPPFDTNKPSEKAAK"
     gene            complement(28002..28868)
                     /locus_tag="ACHALG_000984"
     CDS             complement(28002..28868)
                     /locus_tag="ACHALG_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN2586410.1"
                     /translation="MYDGELIKKLRVNKKLTQTQLAEGICSKTSLVGIETNSVKKVSF
                     GTLKSFLERMNMTLAEYEWIRNQLDEPKKLKKKRRMLDRIQEDSFDPYKEIANNRRHY
                     KKTEDLYYLVLNLHMSKKTEVSETEFEFLRYECRIIEEYLMQVPEYGQFEVSILADFP
                     WIFSDRFIENNYLKIKKRMRRIAQLNNHEQYLFTFLTNLTLFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKSENHQEFFAVIEYTLGKNQRNSL
                     EKKLLAIAKPSS"
     gene            complement(29102..31180)
                     /locus_tag="ACHALG_000985"
     CDS             complement(29102..31180)
                     /locus_tag="ACHALG_000985"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFN2586411.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVSKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPATLGQTEGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTIL
                     YPVTGTNAEQ"
     gene            31673..32890
                     /locus_tag="ACHALG_000986"
     CDS             31673..32890
                     /locus_tag="ACHALG_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFN2586412.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEENTLRSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFSNRLTPYALTLIRLFPHPDFVLASTRTKIKNKLINETREKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQKKALATQMIKKAEASPCFLLYQTFP
                     GIGALTAALLLGELGDITRFKTHKQLNAFVGIDIRRYHSGKYTGQDRINKRGNPKARK
                     IIFFTIRNMIRQQRAAPNHIVDYYYKLKKQPIPKKDKVATVACMNKLLKCMHAMVRAH
                     TEYDYAYAVSVDH"
     gene            complement(33349..33972)
                     /locus_tag="ACHALG_000987"
     CDS             complement(33349..33972)
                     /locus_tag="ACHALG_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2586413.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWEEYKLTANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLVVLLLTHDGDKVARLKNGKVVTAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     ASSQKNESTELSPSDSGSGQTITGNEENQASIITNESSEGQYQTYGQ"
     gene            complement(34128..35525)
                     /locus_tag="ACHALG_000988"
     CDS             complement(34128..35525)
                     /locus_tag="ACHALG_000988"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFN2586414.1"
                     /translation="MKRYQFPKNFWWGSAASGPQTEGRISGDGKGENIFDYWYKNEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HHYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFQLFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKAYHEMDQSGVI
                     GIILNLTPSYPRDESNPEDVKAARIADAFFNRSFLDPAVKGEFPSELIERIKELDLMP
                     EIHPDDLTIIKENTVDLLGINYYQPRRIKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEKGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDRNGFEA"
     gene            complement(35547..36518)
                     /locus_tag="ACHALG_000989"
     CDS             complement(35547..36518)
                     /locus_tag="ACHALG_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN2586415.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMEKISEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISNRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHAIDGLIVFLSEEEWSGFEMLTKMSIPVVALNCPGGPAGIDTIVVNDR
                     ESVAKVIAHHGGQGHKTIGFIAAAQTDYVARERLAGAKEAVQKDPSIHLEIFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            complement(36525..38825)
                     /locus_tag="ACHALG_000990"
     CDS             complement(36525..38825)
                     /locus_tag="ACHALG_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HFN2586416.1"
                     /translation="MYDYPYQNPKLTIRQRVEDLLSRMNIAEKVGQVNQHLYGWECYE
                     KNGDKIELTEKLKEHVKWGGGLGALYGLFRSDPWSKTDYKNGIPAKESWKVANLVQEY
                     VLQHSRWKIPVLLVEECPHGHQGLDGISYPTNIGRGNTFNVALLEQSSRLMAKELAAK
                     GVHLALVSTLDLAKDPRWGRTEECFGEDPILSARFSEAVVRGFQGEIISEKVSFLDQT
                     VEEINKKKDQIGVVLKHCIAQGEALGGHNSGTVTIGRREFSDIYGPLLKSTRNAVGVM
                     AAYNDIDGIPCHINRALFEQTLRKEIGYQGIVMADGVALDRLSDVFTDKKTAAAYALA
                     AGIDLSLWDETYTKIAEAIDNQVVDEKLLDQAVRRVLSVKFLLGLFEQPFANDPKEYW
                     DHLMEESEHLNLETAKESITLLKNDGILPLDQQVKNIAVIGPNAHDVYHLLGDYSAPQ
                     TEDRLKKKIVQEIKTEFPQSKVSYAQGCEVRNQEDQEEKLLEAVTLAQNSDVIIAVLG
                     GSSARNFDMEFLRNGAVSSKGINMDSGENVDVASLSLGGYQEQLIEQLYQLGKPIITI
                     LVQGRPYDLMKIESFTNAIATAWFPGQEGGKAIAQMISGRNNPSGRLSLSYPRNSRQL
                     PVYYYQRAASKQENYYDLSGSPFYPFGHGLSYTTFEYSQMEVKTDIDKVIISIKVKNT
                     GSVSGKTSTLVYVRLIDGVVIQRTKLLKAFRKDELRSQEERLLVFELTSEDFSYMDID
                     DKRHYAKKAVIMVEQQEKEIKCNWQE"
     gene            complement(38827..40152)
                     /locus_tag="ACHALG_000991"
     CDS             complement(38827..40152)
                     /locus_tag="ACHALG_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFN2586417.1"
                     /translation="MKVVDRLSEKMLPIVSKMSSQRHLLAIRDSFIVTMPLVMAASVM
                     VLLNALIFSNATVQRILDLSPLAELASIVNNGTMSILAIIVCYNIGWNLATHYIQTGV
                     IDDPSFSPVHAGILSTAVMFILMSLSSTVTLMDGNTAEATGVFLQSLTSSSGLATAMI
                     AALVSTELFIKLAKMKSFKIRMPEGVPPAVAASFNSLVPEIVVILFFSVVVFTLNHTT
                     GLNVPQLIELAIQTPLKAFVLSVPGILFLQFFSDFLWVFGMHGSSILAPIRQAPLLQS
                     IQENMDAFSVGKEIPNIITEPFTNAFGLIGGGGCILPLVIAILWASKRQEQRSIAKFG
                     LTTCLFNITEPIMFGLPVVMNPIYMIPCAIIPSINLVIAYAATSLGIISKTVAAAPWI
                     TPPVIQSFIATGGDIRAAVLTVILIILDVFLFLPFVLAANKAKLAEGGY"
     gene            40383..40808
                     /locus_tag="ACHALG_000992"
     CDS             40383..40808
                     /locus_tag="ACHALG_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2586418.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DITNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGADSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKEATVTFTKIGNYRLKDKDGNIYSL"
BASE COUNT        12012 a         8725 c         7243 g        13373 t
ORIGIN      
        1 atataattca ccttacttat tgtaagataa agcttttttt aatttattgc ctgctaatat
       61 ataaaaataa ggtctagtta ctgttttttt aaatgattaa taaataacaa ctcctttcta
      121 tgaaattgca aagcattcat cttcttttct gctaacgata tatccgtcaa tgcttgttac
      181 actttactaa aaagtatgag attgccgata tctggatagg tgtgctatac tacagaaaaa
      241 gatatacgat ctgccaccgg gtagtaaaaa agaggatgat cattccatta ggtctttgaa
      301 gggatgattc tcctcttttt gtgtattttt tatattgaag gaggaacgta tgtatggcat
      361 ggatggaatt aatcatagca ggaattttgg aagtattctg gtcaacgatg atgaaatgga
      421 gtgatggttt ttctaaaatc aattattctt tttacacagt gattggaatg attgctagtt
      481 tttacttttt gtcaaaggca atcaaaagtt tgcctatgag tctctcttat cctatttgga
      541 cagggattgg cgcagttggt tcggtcatta ttggtgtggt cttgtttcat gataagctga
      601 atctttccac ttggttcttt gtcgggttgc tgctcatcag catcattggc atcaaaatca
      661 ctagcggtca ctagatactt agacaagcag acaaggactg atcagaaaaa gcggggcttt
      721 ttatttgata ttcctctttc tatgaaaaaa caagaaaata agactaatga cctagcgact
      781 tctctactca tcaggtgtat gcttgtacta tctatagaaa aggagtgaat atttttgaaa
      841 agtctttcgg tccaagaatt atccaagaac tttggtcaca aacaagccct cagcgatgtc
      901 agttttagct tatctcctgg caaaatcgtt ggattagtag gtcctaatgg cgctggaaaa
      961 acaacgatca tgaaagctat tttaggactg atccattact cagaaggaac gatccgtatt
     1021 ggtgaaaatg agatttctcc ctctagtcat aaaggattag aacaagtagg agcattgatc
     1081 gaatatccgg gtatctatcc attcttaaca ggttatgatc atctgaagtt attctctgaa
     1141 acaaacgatg tatctgctat cgacactatc gtgaaacagc tcaagatgga aaaatacatt
     1201 cacaaaaaag cgaaatccta ctcgctggga atgaaacaaa aattgggaat tgctcttgcc
     1261 ttactgaacc atccgtctat cgttatttta gatgagccga tgaacggttt ggatccacaa
     1321 gcaacaagag atgtgcgaaa aatcattacc gacttggcta ctcagggcac ctctttcttg
     1381 atctccagtc acttactcag cgagcttgaa aaaatggtcg atgagctgat cctgatcaat
     1441 caaggaaaaa tcatcaaaca atgtacaatg gctgaattga accaaacagc gcaagatttc
     1501 atcgttatct ctacgacaaa tgatcaagct gctcgaacga tcttgacaga cgctggatat
     1561 cccttggaag atcctgataa aatcaagctt ctaaaaaaaa acgaagagtt attaggaact
     1621 gttatccagt tattgtctgc agaaaatgtc catgtgacag atgtccagca tctgacgaat
     1681 gatttggaat cttccctatt ggaaattctg gattcagtaa agggggaaga agaaaaatga
     1741 cgttgcttat aaaacaagaa ttatttaaat tgatcaagaa aaagtcaact gctgttttat
     1801 ctgttctatt agttgctttg ctcataggaa cagcacttct agccaaaaaa tacacgacga
     1861 tcattgatcc agttgaaatg actgctcagc ttttcagtgc cacatcttgg attgtcttta
     1921 tcatgattgc tgctgcaagt acgatcattt caatggaatc acaatatggt acattgaaaa
     1981 atctccttta tcgaaagtat tctcgaggag aaatattagt aagtaaatgg atcacattag
     2041 ttatttattc tatttattta taccttttag ctattattgt caccgtgctg atgaaactga
     2101 tccttttccc ttcgatttcc tttacggaga agatttctac tggtcagaca ctgatccaaa
     2161 gcttattcac ttatactttg ggtagttata tcggtttatg gctaatttta agtttagtgt
     2221 tgatgattgc ttgttttatc aatagttccg gtgcctccat ctcagcaggg atcgtctttt
     2281 attttgcttc atcaatcata tctggtatcc tcatcgcact cattcaaaaa tgggagtgga
     2341 tcaaatggaa tccaatcagt atgctgaatc tgcaaaacca aattggcaac gaagagatta
     2401 tgaagcaatt gactcatctt tctactaatc aaatgctgtt tggcaattta gcttacattg
     2461 ctttgttctt agcattggga tacctcgtat tcaagcgaaa aaatgtatga tggttcatta
     2521 acaaagaagt tgtgacagaa gtggccagtt tcaagaaatt tcggcttatt tttagaagaa
     2581 gctacttctt gaacaccatt tattcaaaaa aagaagcgat gacacgaatg ttgtcaccgc
     2641 ttcttttttg aataaaccac aggcttcaca gcctctcttt atacttctat acgtgaagca
     2701 tcccagtggc aaataaaacc attgcgatta ctccgaccat cgtcacaccg ataagtaaaa
     2761 tcttatctaa gtggatatgt tgtacaggtt cgttttgttc tttacgtgct ttgaggtaaa
     2821 aataaatacc tggtaagaaa agaacaagaa caagcatcac ttcattaaag gctacaacta
     2881 gcatggccca tacttgaaaa gcacttgcta aaaaccctac taaaaattgg acccactctt
     2941 tccgctgata agaaagcttc atttggtaca gccctaagaa aagccacgtg aagaaaatcg
     3001 ctgctgtacc aagtgtataa gcaaagttat atgcactgtc agtgaataat aatgaaagta
     3061 aaaagacatt cgtacataac gtcgtcaata cgatagcata agttggtgcc ccatttttgt
     3121 tgagttttcc ccatgcttta ggcaaaagtc cgtcctcagc catattcccc attgtttcaa
     3181 tcggcaagat tgtccatgag agccaaaccc caactaaaga aatgatcaaa ccgccgttga
     3241 tcacgaccgc cccccattta ccaaccatca tttcaaatac atatcccata gcaggttgcg
     3301 ctttgatcgt ttgcagctga ttcatcgtca ttgtgccata tggaagcatg gaaaccaaga
     3361 cgtaaataat gatcaaaccg aatattccta gaatcgttgc tttcccaaca tccgattgtt
     3421 tttttgctcg tgaacctacg acagaagctc cttcaatacc aacgaatacc cagagcatca
     3481 ccatgatgca actcaatact tgactagaaa acgggatcgg ctgcccattc gcattcaata
     3541 tgatatcgct agaaaagtta ttaatgaatt gttggatcga aaaggtgctg atagctacta
     3601 caataaataa aaataatggc actaatttac aaaatgtgac gatagcattg acaaagctag
     3661 cattttcaac tccacgattc actagccatg catatcccca taagaagatc gtactcatca
     3721 cgatcgatgc cacattttga ccaccttcga atacggggaa gaaataacct aatgcactca
     3781 tcaacaatgt tgcgaacgcc acgtttccta accagcatgt gagccagtaa ccccaaccgc
     3841 tgataaaacc agcgaaacgg ccaaaacctt cttctgcgta actaaagatc ccgcctttta
     3901 aatctggacg ttttttattg atattattga tcgataagga gagcatcaac attgctgttc
     3961 ctacgagtac ccagctgaag atagctgggc caccagccga tccggagatg tccgtcataa
     4021 taccaaatac gccacctcca atcgctgaac caatcactaa ggcagcaagt ggaatcaaac
     4081 caattccttt tttctgttgc tctgtatcca tgtaagcatc ctccaaagta aaaccgcttt
     4141 tatcaaaaag tataacaaac cttttttatt tttacttaat taataaaaac tttaatagaa
     4201 atataattct accgactttt ttctcattat acaagcaaag cgcctagtcc ctttccaaat
     4261 tacaccacca aaaaaactta aaaagatgct atactaaagg gaaagaattg ggggatgatc
     4321 catgaacatt ttatatatat cgatttcagg aaatacgcgt gcttttgcca aacatctagc
     4381 agaatatgct gaaaaaatgc atgcagaaga tcctgtcaat cctgaagtta ctttaaaaga
     4441 aatccatgaa aatagtgatt ttacgaaaga aacagaacca ttttttactt tcgttcctac
     4501 ttatttagac ggcgggaatg ggttagacaa cggtgacaca gaaatcttga ccgaaacgat
     4561 gcgcgaatat ctggaatacg aagacaatca taaactttgc cttggtgtag tcggtagcgg
     4621 taataaaaac ttcaacaatc aatattgtct gacagccaaa caatacgcac aaagattcgg
     4681 ctttcctttc ttggcagatt atgaattacg gggaacacca tctgatgtga cacgcgttta
     4741 tcaaattttg gcagaaaaaa ataaataaca caaaagaaga agctgaaaaa taaatttcgg
     4801 cttcttcttt ttatgttctc tattgtttct attcttatta agctttgaca tccatcacag
     4861 gtgcttttgc tttcgcttta cctgtttttt ccaagacaaa gttttttaca cgatccatat
     4921 tggttaccaa gacgatcatg gaggtttctt tgtctgctgc tttgattgct tctaagtcag
     4981 cttctgctac aagcgtatca ggtgtcactt gctgtccttc tgttactttc acatcgaatg
     5041 gcgtaccgcc taagtcaact gtgttgatgc ccatgtgaag caagatttct aagccatttg
     5101 gtgtcgtaat gccgatagca tgttttgttg ggaaaacaga acttattact ccagtaaccg
     5161 gggaatagat atgtccgttt tctggttcta caaagtaacc ttcacccatc attttttgtg
     5221 caaaaactgg gtcacttgct ttatctaaag aaacaactgt tccgtcagca actgatgata
     5281 agatagtcac actttcagct gaagcttgag gtactgcttt ttcaggtact tcatttactg
     5341 tggctgtttc ggtcgctagt tcttcttttt ctaaacggtc tttaggaata ccgaagaaga
     5401 atgtagcaac gaagccacca gcgtaagcag ccaataaacc aaggatatag cctaaccatt
     5461 taccatcaga gatcaatgga atcaaagcgg ctcctgaagg tccaatagcg attgctccga
     5521 catttccgat cattccgatc actgccccac cgatacctcc accgatacaa gcagtaataa
     5581 atggacgtcc tagaggtaat gttactccgt aaatcaatgg ttcgccgatt cctaagattc
     5641 gactggtaaa gcccctttga tcatttctgt taattctttg tcttttttgc agcggatcca
     5701 taatgccaat gctgcaccca cttgtcctgc tccggccatc gcaaggattg gaagcaataa
     5761 ggtacgacca gtttcatcaa tcatttgtac atggattggc gtcaagatct gatgtagtcc
     5821 gaacatgacc attggcaaga ataatgtacc tagaacgaag ccggcaaatg ctcctccaac
     5881 accaagtacc cagttgatcc caccgaccat gccgtcagaa atgaaccctg ctaaaggcat
     5941 gatgaagaaa atttcgatca gaccgatggc aatcagggaa agagttggcg taacgatgat
     6001 atctaccgca tcaggtacta ctttatgcag ttttttctca acgattgaca ataaccaaac
     6061 tgcgaagata acacctaaga tcccgccttg tccagttgca agagcttcac cggtaaacaa
     6121 gtttttcagt ggtgcttctg gattcatccc tgtcagcatc acaacagccc cgataactcc
     6181 accaagcgtt ggcgttgcac caaaaacttg tgcagcattg atacctgtat agataactag
     6241 atagctaaac atcccgtttt tcaagatgtt catcacatcg atatattgtt gccatgttcc
     6301 tgcgtctagt gttccagctg tgactaagtt tgctaaaact gcggcaatcc ctgccacgat
     6361 cccagtgccg acaaatgcag ggatcattgg tacaaagata ttagagatgt cttttaatac
     6421 cgatttgatt ttagagggtt tttgtttcga tttttgtgcc gctttcattt cttgtgcccg
     6481 ttcgtttaca cggtcttttc cagatgcaga agaacttgct gtagttgcac tagcagaagg
     6541 aagtttttct cctaatttca cgcctgctaa gtcaaccatt tcctgcgcta ctttgttcac
     6601 tttcccaggt ccgatgatga cttggagctg ttcatcctct actacgccaa gtactcctgg
     6661 aatcgctttt aattccgaga catttacaag atcatcattg atgaccttca tacgtacacg
     6721 agtcatgcag tggacaatgc tttcaacatt gcccataccg cctgctcctt gataaatttg
     6781 ttttgccagt tcttccaaag tcaattcgtt ttttgccatg gtcttttccc cctcataagg
     6841 ttccttttac aaaaccgttt gcttcagcta attttgcttg agctgcctct ttatcactat
     6901 ttgtcaaaat catgacgata gccagtttga cattttgatc tgcttcctca aagtactttt
     6961 cagctgtttc gtagtcagct tctgtcgctt gcatgatgat tcgttttgag cgttcaatta
     7021 gtttttcatt tgttggtttg acgtcgacca tcaagttatt gaatacttta ccgataccga
     7081 tcatactgat agtagaaatc atattcaaaa tcagtttttg tgctgttcct gacttcaatc
     7141 gagtagagcc tgtcaaaaat tctggtcctg catcgacttc gattgggaaa tccgcatgct
     7201 cgctgatttc tgcttgttta ttgcaagaaa tcgttcctgt aacagcacca atcgattttg
     7261 cgtaatctaa tccaccaatc acatatggtg tacgaccgct agctgcgata ccaataacta
     7321 tatcattttt tgttaaattc agcttgatta gatcttcttg ccctaattca taagaatctt
     7381 cggctccttc gacggctaca gtcattgctt cttctccacc agcaattagg cctatgacca
     7441 tttccggttc tacaccgaat gtcggtacac attcagcagc atctaataca ccaagtctac
     7501 cactcgttcc agctcccata taaatcaagc gaccaccttt tttaaaggat tcaatcgtgt
     7561 tggcgatcac tggctcgata cttggcagtt ctttttcaac agcagcggcc actttctgat
     7621 cttctttgtt catgagttgg actgcttgag aaatactcat ttcatctaat ccaaaagttt
     7681 cttggtttct gcgttccgtt gttaggttct ctaaattgat tttgctcatt tttatcttcc
     7741 ttcttttctt ataaattgat agttcatacc agctttgatt tggttgatca gcggtagttc
     7801 ttctttgatg actttggcta ctacattcac tttttcatcc gctggcaaat catatttagt
     7861 taattggatc tcacccatat agcgcaaata cttttcgtta tctaaagtaa tagatccttt
     7921 catacgctta gctgtatttc ttactgggat tggcggaatc tctcgaaaac gcgcatctgc
     7981 gcttcgtatg acatctcttg catcatcttg ccgattcgta tgttttccta atatatattc
     8041 gtaaaaaact tcatctacag gttccgtatg gagcaagact gtctgttctt tttggaaaga
     8101 agcaaattgc tcctgaacgt gttctgacag acctgcatct ccgatataaa caatatccgt
     8161 gttacttgct aatagatcaa gagcacctgc cagaggatgg atccctcgat gttcttccaa
     8221 agttggtaat ccttgataca gtggacctct caactctgta tctcctggta caaagccttg
     8281 tacaaggaat ccttgagttt tgagccattg atttttttcc tgataccatt ctttgtccaa
     8341 ccctgtttca agacgtggat aataattatg ccaagcttca atctgtgaga aatcagccgc
     8401 tgcttctttc aactcatcga tatcattagg cgtgatcgtg ctggcattca aactgatttt
     8461 caattgatgt gaccagtcgg cgatctgctg attagaaata tgataatcca ttcttaaccc
     8521 tgttacacca attgctttca agggtgttag atcttctaat gaaaagccag ctcgtttcaa
     8581 tgcctctcct gaaatatcga ccatcaactc tagtttattc tttttcgctt cgcttcctag
     8641 ttcgatcaat cgattttgat aagccgaaac atcatcttcc ggtatatgca tggaagtgaa
     8701 aataccgaca aagccattgg atgccatttg gcggatatac tgtttcttct cttctgacaa
     8761 agaatcattc ataaatattg aaaagccaaa catattatcc ctctttttct gtatccgtct
     8821 tcatttctat gactagagta tatatctaaa gaaacaaaat ttcaatactt ttgttaaaat
     8881 aattaaattt tatttctaaa aattggattt ataatgccaa aagaattgac acagccacaa
     8941 aaaaggcact gctaagcaaa agcagtgcct tgaaacattt tatttaaata acacaaaagt
     9001 ctaattactc ataagaaatt aattctgtat tgtctaccat gaatgtaaag aacccttcat
     9061 taggcattgg atgaaaataa gcattgatca ctcttatcaa ggcattatga cagactaaca
     9121 agtagacatt ttcttcgtct tccatgcatt ctttcaataa aggaacgatc cgtgcgtaca
     9181 catctaagac cgactctcca tttgggaaac gtacagcaaa cgctaaacgc gctttttgaa
     9241 agttttcatc tttgcttggc agtccgtcat aatccccaaa atccatttct accaatcgct
     9301 catcagcggt catcggcaaa ttgagacgat caactactgc ttgtgctgta tctcttgctc
     9361 gttgaagagg ggaatggatg attttcgtga ttggaacagg caaatctact accttttctg
     9421 ctagtgcctg tgcctgcatc tgtccttttt cagtcagcgg cacatctgct cgcccgcaaa
     9481 ctaatccttg tgcgttccaa gttgtttctc cgtgtctcgt tacataaagc atgtgattct
     9541 ctccttcgtt gacaaagttc tccttaacca taccttgttg tctttgagat tacaaggttt
     9601 agattttctc aagagaaata atctcagtag atactttatc tagaaaggtt tgatcatgtt
     9661 ccactagtaa catagcaggt ttcacgttca aaatcagttg ttctaattgt tcttgattga
     9721 agacatccaa ataattcaat ggttcatccc atatatagag ctcagctggt tgggataaag
     9781 atttagccaa ttccactttt ttccgttgtc ccatactcat ctgttcgatt ttattatgaa
     9841 aaacatctct ttccatccca agctttcgga gattattcaa aaatgcttga tagtctactc
     9901 ggtttttctt cgcgaattcc gccaatgtcc ctcgattgtc ttcataattt tggcgtacgt
     9961 aactaatggt caaatgtttg ggctgggatt tttctcctac aaccttgccg ttgaatgtcc
    10021 ccagaagata atggataatg gatgactttc ccgcgccgtt aggacctgaa attgccatcc
    10081 gctgatgagg ttcgatcgta aaatgaactg gctcgaataa cagattttca taccctaatt
    10141 gaagattttc tacgcttaac agccgtttat gatgagacac ttggctattc atcgtcaaag
    10201 catcgatata ctcgatatct tttaataatt tttctttctc actgatctgg gtttccatcc
    10261 gattcacgat tgctttagat cgtttcatcg tccgtgcagc atcagcgcct actgctcctt
    10321 tattcactcg cctagattcc gtatcgatga atccgacttt tttctttgtt ttgtcccctt
    10381 ctcgggaacg agaccattct gctttttcag ctgctgtttt ctttagcctg ctgacttctt
    10441 ttttcaattt ttcattttta gctatttcaa attcatcacg aagttttttc tgttcttcat
    10501 agatagagaa attcccttga taaagttcca gttggctctt ttcgattgct aaaacatggt
    10561 ccactacttc atcgataaat ccccgatcat gactcaccac gatgaatcct tgttttttct
    10621 ttttcaagta agccgctact tgttttctgc cagagatatc caaatgattc gttggttcat
    10681 cgattaacgg gaaatgagtg tcatccacaa ataagagtgc cagtaggact tttgtcttct
    10741 ctcctcctga tagtgtgctg aattctctcc ataagatttc aggatctgtt tgcatcagtt
    10801 ggagctctct ttcaatttcc catatctcaa aatccataat ctcatttaac acataatagg
    10861 tcaaacgctc tttatctttt gtttgctgcg ggaaatagac aaacgcttgc tgatggatca
    10921 cttgcccttg ataagggagt ttgttttgca ggatattcag taaagttgtc tttcctcgac
    10981 cgtttcgtcc gataagtcct agtttccatt gtgtgtcaaa gtttagattg gcttgttcaa
    11041 ataataatgt gccttggctg tcatagccga atgtcagatt ttttatttcg attttagaca
    11101 ttattccatc acctcgtaaa aaaagaccag tcctattttc agaactggct aaagaggatg
    11161 caagggttgg ggcacacaaa aagacccata catcgaaaga accaagccaa tcccgaaaat
    11221 ctgcacactg ctcccttgtc tccacaaggt acagtctgaa tgcagctttc atcaatcgac
    11281 ttaatttttc aatgcacgga tccctcactt tgtcctatat ttgaaattac gatagcataa
    11341 agaaattgtt tttgtcaact acttcgggag aatgttgaaa aatctgctga gtgtctgtcc
    11401 taagatttct gttttttcta atgtttgcct gtgttccatt gcttcctcta aatcgattgg
    11461 tcctaattga atgcttaaga caatggggag aaaatcaaag agtccttcca gctctcggtc
    11521 tctacttccg caaatagcga ttattggttt tcgatatttt tttgcaagca ctgctacgcc
    11581 atatggaact tttccgtgga ttgtttgctg atcaatccgc ccttctcctg taatgatgac
    11641 atccgcttgt tcgatctgtt tttctaatcc agtgatttgg ctgaccaact caaaaccaga
    11701 gaccatcttc gctcctaaca aaaaaagggc tccgccaata ccgcctgctg ctccgcttcc
    11761 tgggatgtca tctaaatcca cctcccaaac atcttttatc tgctgagcaa tcttttcagc
    11821 ttgtttatcc aagaaagcta acgtcgtatc cgttcctcct ttttgtcttc caaattgcca
    11881 agcagctcct tgttgacctg tatatgggtt ggtcacgtct gcagcgatga tcacttttac
    11941 tttagaaaca agaacgttcc ttcgatccaa atgaagtttt ctagtagata acaaaggatt
    12001 tccttctgtg tacccttcta aagaagcacc caaagattgg agtaatccta gaccaccatc
    12061 gctacatccg cttcctccta aagtgacaat gatttgttcc acttcttgaa gcagtgtatc
    12121 tttgataagc tctcccaagg cataactaga agcaagtcgg atggtttgat ccgttggttt
    12181 atcaagtaaa tctaatccta atacttgtgc agattcaatg actgcggtat tttttccttg
    12241 ccagttcgtc aataaataac tgatttttcg ctgacgaaat aagagatcca tcccatcaat
    12301 cgtacgccag gttcccccga gcgcgtaata gattgcttct gcagtatttt ctccgccatc
    12361 tgcaatcggc acattgattt ttcttccttt acggtcttcc caaccattta gaactgcctg
    12421 atttaattca aatgaagaag cacttccttt aaatgaatcg attgccgcca aaatatccat
    12481 ctatatccct cactttcttc ttaagtaaga cacttttttt cacttttttc aaacaaaaca
    12541 atgctatttt cttctgtttt tctatatact aaaagggaac tcattgaata aaggagagaa
    12601 aaaacatgaa aatcgcaata gctggagccg gagcaatggg cagccgaatc ggattaatgt
    12661 tgcatcaaag cggaaatgag gtcgtcttga tcgatcgctg gcctgctcac attgaagcca
    12721 ttcgtacaaa tggattgatt gctgacttta atggcaaaga agtagttgca aaactgccaa
    12781 tctattcacc agaagaaatc atcgaaagca acaaacacgt ggatttgatc gtagccttga
    12841 caaaagcgaa tcaattagat gatatgtttc gttcgatcca atccattatt actgagaata
    12901 cttatgtttt gtgtttactg aatggattag gtcatgaaga tgtactggaa aaatatgtac
    12961 caaagaaaaa tatcttattt gggatcacga tgtggacagc tggtctagct ggtcccggaa
    13021 aagtcacatt attaggtgat ggagaaatcg aactagagaa cttggaacca gaaggcgaag
    13081 catttacaaa aaaagttgta gaagtctttc aagaagcaaa attgaatcca atctatagtc
    13141 ataatgttcg ttattccatt tggcgaaaag cttgtgtaaa cggaacatta aatggattat
    13201 gcacgatatt ggattgtaat attgctgaat taggtgcgca aaaagcggca gagggcatgg
    13261 ttcgaacgat tgtatctgag tttgcttcta tcgcagcaaa agaaggaatc acattagatc
    13321 aagaagaagt gtaccaacat attgcttcta cttatgatcc tgacaacatc gggttgcatt
    13381 acccatctat gtaccaagac ctgatcaaaa accatcgttt gactgaaatc gactacatca
    13441 atggtgccgt ttggcgaaaa ggacaaaaat atgatattgc tacaccatat tgtgcctttt
    13501 tgacccaatt agtccatgca aaagaaggaa tccttggcgc agaataaaag gctctctgat
    13561 gagaatcagc tggatcagca gctggttctc atttttttac ccccatgaaa gttcaaaaag
    13621 aaaaacacaa ttctcatgtt gccataagaa ttgcgcttgt ttattttcat ctatgattgg
    13681 ttggcacttt tatttatttt taacagagga aagatcgtat tcatattgtt cataaatagc
    13741 ggagtcagta gtgaaaaaac gttttgccca attttctgca tcaaaaaatt ccaacagttc
    13801 tgatgggatt ggttgaaatg gcgtatgcaa aaattccggt aattcatcta aatcacgttc
    13861 ttgaagcaaa aaaatatttt cagaatgata aaactgataa tgagctatgg cttgattatc
    13921 tgtcactagt ttcttttgaa aatacataga ttcaaacgtt cgcaaagtca ctccttgctg
    13981 cccttcccgc aacagttcta gaatccccct cgattccccg gtttttttca gatattcttc
    14041 ataagtaagg tatccaggtt gttcatttcc tcgaagcgga agaataaagt aattggtcgt
    14101 aacacctctt tttgtaaatt cctgcttata tttttctgcc cgttcttttc tgccgttatt
    14161 tgttgcacca aaaaatagat ccgtctttgg ttcgctagaa gaaaaatcca tatgtttcga
    14221 ataaaaggaa ctattatgtt ttagtccaag ttctttcgct tctaaaaaat caaaatgata
    14281 aatctcatcg attgccggtt catcttgtgc ctgcttcagc atcgcctcgt gttctgcttt
    14341 aaagtggttc caaaaaaata acacgatttt tgcattggta aatggacgca cgcgtcgagc
    14401 catctccata tcaaatccat aatctaaaaa aacgatcagc tgataatttg ccaactgttg
    14461 tcgagctgat ttaatgaatt ccgactgtag taaatctttt ccggaccaaa catctaaaga
    14521 gcggatggaa ggaaaaaaat aaagtgattt atttttcgca attagcaata cgtcgttttt
    14581 gttcatctgt tccgcctcac tttctctgtc actttttcct atgatagcct gtttaaatag
    14641 taaaaagcca gtaaaacgct tcctattatt tttataaaaa tttaacattt ctatgaaaat
    14701 acgatgaaat aaatcattct tccattaaaa tatgcccaaa agttttctga atagttgtaa
    14761 ttatctgaaa atttgttatc attagtcatt attattatta ctatttattc ttactattta
    14821 ttcttactat gcattttgat tgtgaattta aaagaaagag gttatttatg gaagataaaa
    14881 aattgaaaaa acaacttagc tcccgacata tcaccatgct agctttaggt ggagcgattg
    14941 gtgcaggtct atttaaagga agtggcgaag cgattggaat cgccggccct tctgtattga
    15001 tcgctttttt gattggcggc ataattttat atatcgtgat gaaaggtctg ggaaaaattg
    15061 ttttaagtgg tggtgatacg caccacggat tatctggatt gattcgtcca tatctaggtg
    15121 ctcatgctgc cgattttacc gattgggtct actggtccat gtggattatc aatatcattg
    15181 ctgaagctgt tgcagctgct tcctttttac agttgtggtt cccccacatc ccagcttggg
    15241 tatttgtttt tatgttggct gttttaacta ctatcatcaa tctttattcg gtacgattat
    15301 tcgcagagac agaatattgg ctggcctttg ctaaaatcag tgtcattatc ctattgatca
    15361 tttttgctgc ttatcttgtc ggtcagcaaa tgttgggtac tggtgttttc cctacccttc
    15421 aacaattaac tgatcatggt ggttttactc cccacggtat gaagggaatc atcagttctt
    15481 tgcttgtagt tatctattct tacggcggtt ctgagttgat tgctatcaca gtaagcgaag
    15541 cagatgatcc taaacgtgcg attcctaaag caatcaaagg cgtcatggga agaatcattt
    15601 ccttttacat tattccttta tttttgcttt tgattatttt tccttggaat actttagcag
    15661 gcacgaacgt tagtcctttt gtgatggtat ttgaaaaaat gaatattcca tttgcggcag
    15721 atatcgtcaa tttcgtgatt gttcttgctc tgttctcgtc tatcaattca ggtgtatatg
    15781 cttcttcgag aattttgttc ttccgcttga aagatcggaa aggttcctct agaaaactag
    15841 ctgttttaaa caagcatcaa gtaccgcaac gtgctgtgct gttttgtacc agtgttcttt
    15901 atctaggtgt ggctctttct tattttgtcg gtgacaaatt atttggctat ctggcaggtt
    15961 ctctttccta tacagtatta ttgatctgga tcattatcag tgctgcgtcc tttgtactga
    16021 cactaaggaa aggaacgtta tgggacaaag gaatcagctt ttttgcatta gcagtcttat
    16081 ggttgatttt ccttggtatc ctattcacga actccattgg ggtaactgta ttgaccggtt
    16141 tgctttacct gttcatttat ttcagttatc aaaaaaagaa tgatgctttc gtcttgacga
    16201 atgaatgaaa ccaatagaga attttattct atttatattg aatatctgga gctattgaaa
    16261 aaaaggagct gcgacaaaaa ttgttgcagt tcttttttcc gaataaacgg tgttcaaaaa
    16321 ccatctgtgc agattaattg attgttctcc acgctcatga catgatagaa gaaaggaatg
    16381 aagaaaaagg agtttgtttt aaatgaaaaa gtattggaag tttttgttga tgattggtgt
    16441 ttcgacggta gtcatgtttg gtatgatgta tttgaatgtt tatgcgcttg atcatatctt
    16501 cttcagtcgc acacgtgtat tcatggcgtt gatgatgggt gcagtcatgg cagtcatcat
    16561 gttgttgttc atgtggaaaa tgtatgaaaa caaaaagata aatgctatga ttttagccgc
    16621 tagtcttctt gtttttgccg gttccttatt tatggtacga agccaaacaa ctgtcaacga
    16681 tacggcgtgg atgaaggcga tgatccctca tcactctatt gctatcttga caagtaaacg
    16741 agctgattta aaagatcctg aagtaaaaaa attagcggaa gaaatcattc aggcacaaga
    16801 agaagaaatc gacaaaatga aaaaaatgat caaagaaatg gaatgaccat tatccatcca
    16861 aataaaaaaa gaggatgttc cagaagtgat caacttcaag aaataagaag aagctacttc
    16921 tggaacaccg tgaatcagag gttgtgaaaa agctgccctg catcaagtaa taagaacagc
    16981 caaacaaaaa tagcttctca tatttttcgt ttggttaggc ttattaccgc aaatgcaagc
    17041 ttttgaacac cgtttattcg taaaagggag tgtgacaaaa gtcttgtcac actccctttt
    17101 tacgttttta ttgttctaat tgcgctgtct tgcttactgc ttcttgcggt ttcacaaatt
    17161 tctttgagat cccccaacct gtaaaggcac tgaccatcga aaacacaggt gaaagcaaac
    17221 tgaaaaacac gaatggcaag taagtgagtg taggaatgcc aaatgtactt gctacgaaac
    17281 ttccggcgat tccccaaggc actaagtagt tgattactgt tccaccatct tctagtgttc
    17341 tccctaatac agttggatca aatcctgctt tttcgtaagt ttctttaaat gcattaccag
    17401 gcaggataac agatagaaat tgttctccaa taaagatatt tactccgatg cttgtcatca
    17461 atgttgtaaa aatcaatttt ggggtgttcg tcatctttct tgatactcgt tccataacgg
    17521 ttgagatcaa cccaaattcg atcaacaaac cacctaatga caatgttaga ataatcaatg
    17581 acacagttgg catcatgctt tcgattcctc cacggctaag tagtgaatcg atttgttggt
    17641 ttccagtatt tgaaacaaat cccgattcga tgacccctgc caacttcgtt aaggaagttt
    17701 gtgggttttg aataaagatc attcctactg ctacagcaat attcaacaaa atcgtcaaaa
    17761 ttgccggtac ctttttccat gcacacaaga gcattaaaaa tagaggaatc agtgaccaga
    17821 tcgaaatagt aaagtttgct tctaaaatat cagtcacttc acgaatagat gaaagacttg
    17881 cggctttact tgtgtgtcct aaaatcatga ataaaaagaa agaaacaaca aatgccggaa
    17941 cagttgacca catcatatgc ttgatgtgtt tgaacaaatc ggcatcaaca atagctgctg
    18001 ctaaatttgt tgattcggat aatggagaca tcttgtctcc aaaaacggca cctgaaacaa
    18061 tggctccaac aacaagggct ggatttagtc ctagtgttgt cccaataccg aaaaatgcaa
    18121 tcccaatcgt tgacataaca gtaaaggcac tccctacagc gctacctacg attgcacaaa
    18181 caacaaatac agaagggacg aaccactgga cactaatcaa tttaaatcca atgaccatga
    18241 gggttggaat aattcctgct tgtatccata cagcaatcaa tgaaccgact aaaataaaaa
    18301 taaagattgg aatgatcccc ggtttgatgc cattgatgat cccttcgtgc agccaatctg
    18361 tcgggtattt ttttatcact gcgtacaaca tgaccattcc gatagctgcc aaaatcgtga
    18421 tattaggtga aacctttaat ccgacaacgc caatcgcaat gcctaaaaca atgaccatca
    18481 atacaagtgt tgcttctaaa aaacttggtg ttcctttttt ttccatgata atctctcctt
    18541 ttttagttgt tcttatgttc taaaaaagtt aagatatcgg attactccgc cacaattcat
    18601 ccgcgattcc tcctttataa aaataaaaag acctgctttc aactaaggac gaatgactcc
    18661 gcggtaccac cttaattaaa aacagatctg tttttcactt tcaaacgatt attcaaatgt
    18721 atggactttc catgtaattc gtcaattagg cttgatcggt tcacagcgac caccgacttt
    18781 ctgaacaaaa cacctaacca ctactgcgtg aaaagttgac ttgtatgaag tttaccttcc
    18841 ataagatcat ttgtcaatga aaaagaaaat attttttcat ttttctgaaa attattttta
    18901 taactttcgc gcacaacttt atctttttac tagaaagtag cgccctcttg tttttttctt
    18961 gctactattt cttgatgttc ttgtttgatt ttttctaata gcttgggttc tctcaataaa
    19021 tctagagctg tattggcaag taattccgct gcaatcgcaa tagaagcaag tcctttttca
    19081 cttttagcag cagctttgaa ttcttctgta tgtccggcaa tatatgtctc agagattgct
    19141 acagtaggct gtatcgttgg aataacttgg ctaacatttc ctacatcaga agaacctaga
    19201 cttgttcttt gttccgtttc tatttcttca tcaggaaccc ctgcttcttt tgcatgttgg
    19261 aagaataagt catcgaaaga aggcgttacg atgacatcat cgactgcatt ttggaacaat
    19321 ccaaactcaa aagtcgtacc cgtgcttaat gcagcacctc gtacgatatt ttccactttt
    19381 tcatatactt tgtctaatgt ttgtcgattt cctgcacgta aatagaaacg tccagcagcg
    19441 tagtctggta tgacattggc cgccacgcct ccatctgtaa tgattccatg gatattgaca
    19501 tcttttggca attgcaagcg tagagcgttg attccgttaa acacttggat caaggcttcc
    19561 aaagcattta ctcctttttc aggtgctgct gctgcatgag aagcgacacc gtaaaatttg
    19621 atatcgactg gatcatttgc taaagattct gtcgttttcc cataacgaaa agctggatgg
    19681 acacataatg ctgcatcgac atcctcaaaa aaaccttccc gaacgaagct tcccttagct
    19741 gatccatttt cgcctccttc ttcccccgga gtaccataca cacgaatttc tccgccaatc
    19801 tcatcaatga attgtttcac tatgctggct gctaatacgg agtaagtacc aaataaatta
    19861 tgtccgcatg catgaccaat acctggtaaa gcatcaaatt ctgccaaaaa ggcaattgtc
    19921 ggacctggtt tattggaaat ataacgtgca tcaaagcctg ttcggtgccc agctacttct
    19981 ttttctaccc agaacccttc tttttccaat tgtttgatta aagctgctga ggaataaaat
    20041 tcataattac ttacttcagg atggtcgtgg atagctagtg caagtgcttg atattctggt
    20101 aaacggtatt gaatcagatc cttcaattga tttgttgaga gtgtggttgt cataaaaatt
    20161 tcctcctatg ctagtcgttt tttcttattt tctcccgaat gtttcccacg caggtacgct
    20221 tgaacctttt gatgtttttt cgattacttt ggctgtttct tctgtttgat aggcatctac
    20281 tattttttga tacgttttat tgtcttcatc ttcttttctt gcaacgatga tattgacata
    20341 aggacgtgca tgctcatcga ctggttctaa gaagattgca tcagaagcgg gattaaatcc
    20401 agcatctaca gctacaccgc tattgatgac tgaaatatct acatctgaca aagcacgtgc
    20461 tgtttgggaa gcatctaact cagaaatgtc tagatttttt ttattctcgg taatatcaga
    20521 aacggtaggc gcttggcctt ttgccttgtc cactttgatc aatccagcag tttgcaataa
    20581 cagaagagct ctgccgccat tcgtgacatc attagggatt gcaacttttg ctccatcttt
    20641 gatatcagcc acatctttta acgtattgga ataaattccc aacggtgcat taaccgtatt
    20701 cccaatcgat actaaatcag ttccgtgttc ttcattgtaa ttgtctaaaa agaattgatg
    20761 ttggaaggaa ttcaaatcga tcgatccatc tgctaaagcc gcatttggct gattgtaatc
    20821 tgtaaatttc acatattcca aatggatgtt ttcctttttc aaacggtctt tcacatcgtc
    20881 ccatacatct gtatcttccc caatgatccc tagttttacg gtcgtttctt ttttttcagt
    20941 tgtagctgct tttccttggc tgcatcctgc caaccaaata attgctacaa atgtaatgac
    21001 tgcaaaacga ataatttttt tcattttttc tcctccattt tcaatgtgtg acttttttaa
    21061 tcagttgatt actgataaat tgactcaaaa aaactaagac cagaatcaat aatgtagcga
    21121 caatcgttac atctgtttgg aatcgattat atcctctcga tatagcaaga ttccctaatc
    21181 ctccggcacc tactgctcca gccatggtag tcagtccgat cacattgatg atcgtcaacg
    21241 ctgagacccg aatgatacca ggtaaccctt ctaacaaata aacacgtaaa atgatgccga
    21301 ttgggctcgt gcccatcgct tctgctgctt cgatcacccc aggatcaact tctagcagtg
    21361 cattctcgat ttgacgggca aaaaacggga caacgccaaa aatcaaaggg actaaagctg
    21421 ctttggcacc aatcgtcgtt ccaactaaga aacgagtagt caaagctaaa agagaaagca
    21481 agataatgaa agggatggaa cgaatgatat tgatcaactt atcaagtatt tcgaacagca
    21541 gcctgttagc tagtaatcca tttggacgtg tcactactaa cgtcacacct aagatgattc
    21601 cgatgatccc accaatgaat gccgtccaaa aagacatata tagtgtttgc tttgtcgctt
    21661 cgatgaattc atccggaatc aaatagacat tcggcaaata tgttttaaaa aattcagtca
    21721 ttttagtttc ctccttcgat gactttgata cggttatcta attcattttt ttgtaaaata
    21781 gttactttta caccagattt aatcaaataa tccaatgctt tttgccgctg tttggtttct
    21841 ccagctaaag taacgatcaa acttcctaag ggtgtttctt gtaatagctc tacattaccg
    21901 tataagatat tcgctgttac ttggtacttg ctgtaaagat gagcgattag aggttcgttc
    21961 gtctggttgc cgatataact taattccacc aaccatttat ttgcaatctg atcggcaaaa
    22021 gcagcatggg cactgattgt ttccaatgct tggtcaaggt gtgtcgctgt ccgaataaaa
    22081 tctttagtca attcttcttc aggatgactg aaaatctgga tgctgctacc tttttcaatg
    22141 attttccccg cttccataac cgctaccttg ttgcagattt ctttgacaac ttgcatctca
    22201 tgagtgatca ggacgattgt tagacctagc tgacgattta gtttttttaa cagtgcaagg
    22261 atctgaaggg tcgttttcgg atcaagtgca ctagttgctt catcgcataa caacacttta
    22321 ggttcatttg ccagagctcg tgcgattgcc actcgttgtt tttgcccccc cgacagttgt
    22381 ttaggatacg cagatgcttt ttcttccaga ccgacaagtt ccagtagttc attcactttt
    22441 tgacgacgtt cctgttttga ttttccagaa tactttagag aaaaatctac gttgtcaaaa
    22501 atcgttcgac tttccattag attgaaatgc tgaaagatca taccgatggc ctttctttct
    22561 tttcgcaatt cttttggaga gagctggctt aaattcgtct gattgacgat gacttctcct
    22621 gctgttggtt tctgcaacaa attcatcacg cggactaacg tactctttcc tgcacctgag
    22681 taaccgacga tcccataaac atcccctttt tcaatcgtta aatctacaga atctactgcc
    22741 tgtatctctt tttctttttg tttgaaacat acagaaatat ctcgcaattc aatcattaac
    22801 attcctcctt gtctataaaa aaagcatcct caagaaaatg aaaatagtca ttttcttgag
    22861 gacgcctttt tgcgtgttac cacctcatgt tcaagctgtg ttcacacaca actcctcttc
    22921 aagtaccatc atactctagc aagatatcgg tcgcagccgt caccgcctta catctatgct
    22981 cgggatgaat tgctcgatgg ccattttcca atagtcgttc cttgcttctt ctcagctacc
    23041 gaagctctct gtcaaaggtt cttctatcgt acttaccatt tcaatgcaaa taatattcaa
    23101 aaaaaaaatc cttttactaa cataaactat gtcagtaaaa ggacgacgta tcgtggtacc
    23161 accttttttc ggaagtttcc ttccctcaag cccaacatct caaaaagaga gattggcgtt
    23221 cgttttaacg gtcgaacatt tccgaatttg cctacttatc gacaaattat tctccaaggc
    23281 cattttcagc ttttcttact tgactcttca caccagccag agcctctctg caaagtttcc
    23341 aagcttactt tccttctcaa cgaaatcaat attttgttgt tgggaataat ctacaggagg
    23401 aaaactgaaa agtcaagcat gaaatccaaa ttattttttc tatatttatt tttctgtttt
    23461 tttatctaac atttcaataa cagttcgaag atcttttcct tccgcaagaa ggttcaattt
    23521 ttccttctct tcgacgctat aatttcgaac aaaactggaa tcgggaatca aaacaacgcc
    23581 tagtcctgca cgtgaagcgg ctgtcgctcc tactaaagaa tcttccactg caaaagctga
    23641 aagagcttct aatcctgttc gttccaaagc tgtcagataa gggactggtg aaggcttgtt
    23701 ctccgatact tcatctccga acgtaaacgt atcaaagtaa tcggctaatt gaaagtgatc
    23761 caagatatct gttgctcttt tcgtaacagt tgtcgtagca atccctagtt tcatatgacg
    23821 ttcttttaag tctgtcaata tttcttttgc atagggtttc aattggatag ctccacttgt
    23881 cagctgttga taaaaatatt cttcacgctt tgctcggact tcctgcactt tttcaggact
    23941 gcctgctgcc tgcactaaaa tatcaaaact ttgtttccag ttttttccgc cccaagcgtc
    24001 aacgatttct ttcgggatat gaatatcaaa ttgctggaat ccccaaagcc aaccatcccg
    24061 atacgttctt tccgtatcca ctaacaaacc atctaaatca aatattgcac ctttcatcta
    24121 tacattaatc ctttccaaaa caatacacct agtttagcat gactgatcca aaatgaacag
    24181 aagcatagag cccagccgca tcacttataa gagttcttgg aatcgacgga tatagatttt
    24241 cttcacagct gtcacaagcg ccagataagc gacgactgtt ataaacaacc ataaccagta
    24301 attgccaggg agcggcatca agcctaactc agcaccaaat cctgtgaatg gaaggactga
    24361 accgacaatg attcctaatg tagtgataac cagcatggca aatgttgcat tgctttggat
    24421 aaatggtatt ttcggtgtac gcaatgcatg gagtactaat gtttgtgtcc acagggattc
    24481 gacaaaccat cctgcatgga ataatgcgat aaaaatcact tgttgtgagc tatccaatgt
    24541 atggaaactt ccaccaacta cagcaggaca aataacaaaa tacatcaaca agtaagtagt
    24601 gatatcaaaa atcgaacttg ttggaccaaa ataaaccatg aattttccaa tactagatgc
    24661 attccatttc tttggctcat ccaaatattc tttatccatc cgatcccatg gtaaggaaat
    24721 gcaagaaacg tcatagatca gattcaaaaa tagaagttgc aaaggcaaca ttggtaaaaa
    24781 tggtaagaac gtactggcaa tcagtacgga aaacatattg ccaaaattag aacttgctgt
    24841 tgccttgata tatttcatga tgtttccaaa agtttctctt ccagaaagaa ggcctctttc
    24901 caaaatcgtc aaatcttttt ctaaaaggat cacgtctgct gattcttttg cgatatccac
    24961 agctgtatcc acggaaatac cgatatcagc cactttcatt gctggagcat cattgatacc
    25021 atctcctaaa aatccgacag tatgcccatt tttacgtaaa gctgtcgtca atcgtgcttt
    25081 ttgctgagga ttcaatttta cgaatacttc gtgttcttca gctacttgcg ctaattcatg
    25141 atcattcatt tccgagattt tctcaccagt aatcaattca ttgatttcta ggcctacttg
    25201 acggcacaca gaccgtgtga ctaattcatt gtccccagtc agaactttta cagctacacc
    25261 atgttctttt aaagctttca acgcttgttt tgttgtttct tttggtggat caaggaaagc
    25321 aagataacca atcagaacca tgtctgactc atctttgact gaaaattctc caacaacgct
    25381 tggattggtc ttttgtgcga caccaatcac tcgtaagccg tcttcattaa gtgattgtac
    25441 ttttgcaagc accgcttctt ttcgttcttt tgttaatggt gcgacagtac caccaagatc
    25501 aataaattta gaaatgttca acatttcttc aatcgcgcct tttgtgatca tctgtgtttt
    25561 gccagccgga tcagcgacta caacactcat acggcgacgt tcaaaatcaa aaggaatttc
    25621 atctactttt tgataattga ttgtatctgt atctaaagtt tctgctgctt catcaatgat
    25681 agccacatct aacaaatttt tcagccctgt ttgatagtag ctgtttagat aagcatggcg
    25741 caaaacacga tcatcttcat ttccttcaca atccatatga tattcaagaa tgattttgtc
    25801 ttgtgtcaat gttcccgttt tatccgtaca taacacatcg atcgcaccaa aattctggat
    25861 agcatttaaa tttttgatga ctgttccttt tttggccatc acacttgctc ctttgaccaa
    25921 attcgtcgta acgatcattg gaagcatttc aggcgttaaa ccaactgcca cagataatcc
    25981 aaaaagaaaa gcttctaacc aatctccttt tgtaagccca ttgataacga taactgctgg
    26041 agccatcaat gacataaatt tgatcagcag catggatgtt ttgtggatgc ctaattcaaa
    26101 acttgattgg atgggttttt cagatagcgt ttctgctaca tgcccaaata gtgtgtcatt
    26161 tccgacagaa acaatgattc cttccgcgct tccactgatg acattgcttc ccataaagac
    26221 aagattttca taacttgttt cactttgctg atcgtcttca catactttcg catgcttttc
    26281 tactggatag ctttcccctg tcaatgctgc ttgagaaatg aatagatcct ttgcggacaa
    26341 taaacgcata tccgctggta tcatatctcc tgctgaaagc cgaacaagat ctccacagac
    26401 gatttcttcc ataggaatct ctgtatactc gccatttcgt ttgacagctg ccgttacttt
    26461 cacaagtgat ttcagttttt cagctgcttg attagaacgc acagactgga tcaacgtcat
    26521 cgtcccactg acgataacca tagtgaagat aatggctaca ccaaagagat ctttatctcc
    26581 tggagcggct aatacgtatt ccgtaatgaa tgaaataatt cctaatgcaa tcaacactaa
    26641 tgtaaagggt gtgatgtatg ctttcaatat ttctataatg aatggtgttt ttttgccata
    26701 tgaaatcgca ttttcaccat aagtttctct tgaacgctcg atttgttctt ctgacaatcc
    26761 atttggtgat gcatggaaca attcaaataa ttcttgcttc gtttttccag caaaagctgc
    26821 atagattttt tgtgtatctt tctttttttc catctcgatc tctcctttgt gtccaaaaga
    26881 cagctgttct cgctgttttt ggtcttttgc tattcatcgg atcgatagag aaaatgctga
    26941 aaggggctga gaacagatag ataatagccc tggcctttcc tctaaatcct tgtttttcga
    27001 atgactgtca tccatttgtt ctcatctcct ttactaagta attttaattc atgacttcca
    27061 atcatttttc gaatagttat tttcccaact cgctcacctc cctttttgtt ttttcaccta
    27121 tgggatttgc ggcaacaaaa aaacctctcg ccaaatactc agcgaaaggt ttgaaaaagc
    27181 aaatcaaaaa aagcttctca gatctcatcg ttgagttttg acactgtaca acgtaaaaag
    27241 cagatatctg cttttagcta ccttatgaaa agccttatct cgacaattcc tgttctaccc
    27301 attggcgtct ctcgacgttt ttgggcagta gcctatgttt gtataggagc ctcacctaac
    27361 aaatcctatt cggttttccc tcgttaatat aacaactaaa aaagaggatg tcaaatcaat
    27421 tttccatgtt tttcatactt ttttaaaata cacgcgttat atttcctgtc tcacgcagtt
    27481 ttatttgtct cattttccac ctcccgctat aatagaatta tagtaggaaa cgaggtgctg
    27541 ttatggaacg aacacttgtt attatcaagc cagacggcgt aagacgccat ttggtcggaa
    27601 gcatcatcca acgttttgaa gcaaagggac tggccattgc tgaaatgaaa tttgcaacga
    27661 tgacacctga actagcaaaa gaacattatc agcacttgac tgaacgtagt ttttttgatg
    27721 agcttatcga ttacatgact tctggtccgg tcgtttatct tgtgctggtc ggcgaggaag
    27781 tgatcgatat cgtccgtaag atggtcggag caacaaaagg ggcggatgcc gttccaggta
    27841 cgatccgagg agattatgca ctgccgggga ctgaaaacat catccatgct tctgattccc
    27901 gtgatgctgc aacaaaagag atcgcccgct ttttcccgcc attcgataca aacaaaccgt
    27961 ctgaaaaagc ggcgaagtga tacaaatcct aaactgttgg tctaactgct gggtttagca
    28021 atggccagca gttttttctc taaagaattc ctttgattct tccctaatgt atattcgatc
    28081 actgcgaaaa actcttggtg attttccgat ttttcttccc ctaaagcagc agaaatcagt
    28141 cgttggtaat aatccgccat caatgtttca taaatcactg tacttctttc tttatgcgct
    28201 aaactccgtt tcatatcttc attcacgcta cgtgcctttt ttaagcgacc attttctata
    28261 taaaacaaag tcaagttcgt caaaaaagta aagagatact gctcgtgatt attcagttgc
    28321 gcgatccgtc gcattctttt tttgattttc aaataattat tctcaataaa acgatcactg
    28381 aatatccaag gaaaatctgc tagaatagat acttcaaatt gcccatactc tggtacttgc
    28441 atcaaatatt cttcaatgat acgacattca taacgcaaaa attcaaattc cgtctcagat
    28501 acttctgttt ttttggacat gtgcagattc aaaaccaaat agtacagatc ttctgttttc
    28561 ttatagtgcc tgcggttatt agcaatttct ttgtaaggat caaagctgtc ttcttgaatc
    28621 cgatctagca ttcgtctttt tttctttaat ttttttggct catcaagctg atttcgtatc
    28681 cattcatatt cggcaagtgt catgttcatt cgctctaaaa atgatttcaa tgttccaaac
    28741 gatacttttt tcactgaatt tgtctcgatc ccgactagcg aagttttaga acagattcct
    28801 tccgctaatt gggtctgtgt cagctttttg ttgactcgaa gcttcttaat caattctcca
    28861 tcatacataa tagttctcct tttttaattg aaattaaccc ttggcttttc cagaatgctt
    28921 gccaataaga ggaatgatta tttcaattta aattttccgc attcaataaa tagatacgtt
    28981 aaaaataaaa aatacacgaa aaaaataaaa aaatgttttt ctcataaaaa ataccaacct
    29041 tcaagcatga gactgctgtc taatccctga aggttggttt gattgtttat tgatcatttt
    29101 tttactgctc cgcattggtt cctgttactg gatataggat agtcgttgtt ttattgccgt
    29161 tttttgaaaa gacactggtc gaatgttcac ctttttgctt ttcgattgct tcattacgtt
    29221 ccgcttcgtt tttatagtca tattttttcg gatcaatcgt ctcaaatccg tttggcacat
    29281 aaaaccgcaa gagattttcc tgattcagct tatctgaaag acttaacgca gtcttgactt
    29341 tttcctgatc tttggagatt tcctcgtcga ttcctgctgc tttcgtatcg atgatttctc
    29401 ctgtctgatt attgtaagct tttcctccta ggacggtgta tttgggcgtc acgaagttcc
    29461 catttcgaaa agcaactact tgatcgtgct gagaagataa taagtctgta ccaaaatgaa
    29521 gataatcttt atcatcgatt cccaatagat gtagtaatgt cggcaaaaca tcgatttctc
    29581 caccatattc atgattgact gtccctttag tgtaaccagg catatggatc atcaatggca
    29641 ctcgttgcat ctgtgcgttg tcaaattcat cccatgtatc tggatcttta ccaagagcac
    29701 ttgctaaatc tttattatct gtattcgaga tcccaaaatg atccccgtaa atcacaaaga
    29761 ttgaattttg gtaaatacca gattttttca aatacgtaaa aaattgttcc aatgattgat
    29821 ccagataatg ggccgttcga acataattat ctactgtgct attgccagta ttcaaagatg
    29881 gaaagtcgaa attcttgtca tccgtataat aaggcgtgtg gttggttacg gaaagaaatt
    29941 tcgtgtaaaa cggctgttgt agatgttcaa gatattgggc agattctctg aataaatctt
    30001 tatctaatat cccataacct aacgtttcat cggattcatc aaaataagat cgatcaaaga
    30061 aattgtcata ccctaaattt ttatatacat gatctctatt ccagaaacta gctacattcc
    30121 catggaaaac agcacttgta tagccttctg tttgaccaag ggtcgctggt gctgcttgaa
    30181 agacattatc tgacccaagt tctgtgaata atgagccttg aggtaaacca aatgtgccag
    30241 tttccaacat gttttcagca tcacttgtct ttccctgtcc tacttcatga aagaaattgt
    30301 caaaactgat cgttgcttga ttctggaaaa tactatttaa aaacggcgtg acttcttgtc
    30361 cgtctacctt catattgatc aaaaactgtt gaaaactttc taaatgaagg acaatcacat
    30421 tttttccttt agcaatccca aatgtttcag gatttggctc tgcataatgt ttctttgtat
    30481 agtctaaaac ttcatcaatt ccattactgc ttgcatgcgc gcgaacactg ctagtcattc
    30541 ctgttttgat tccgtcataa atagtgtacg catcgattcc tagatatttg acaatatagg
    30601 aacggtcaaa tgtccgctgt agcaattggg gacggttggc ttcgctcaac gctaaatttc
    30661 ctgaaaaaat caatcctgaa agacaggtaa cagcgatagc catcggtttg ctgacagggt
    30721 tgcttttgat aggtattttc ttgaccttca accaaaccag tagcccaatg aacacagcga
    30781 tatccaacca atagatgaca tcatggggtt tcatcaaaga aaaggagctt cctgacaacc
    30841 cttgtgacac tttcgaaaag cctaatacag atttgactgt aatgaaatct gtgaattccc
    30901 gataaaaaat aatattcaag tacaacaaaa gcgtatttaa aatatcaatg atcaaaagaa
    30961 tggctaacgc tggctttggc tttttaatat acaaagaaag gccaaaaaac aataaggtag
    31021 tcgcaatcgg gttgatccac aagataaagt actggatcgt accttccaca cctaatgaaa
    31081 aatctgcata gtaggcaatg atcgtcttca gccagaaaaa gaaaacagct gtcaaaacga
    31141 ttgctaaacg attttggcac aattttttaa gttgtgtcac gtatatcact tctctctatt
    31201 cacatttttt cttattttgc tcctgaaaaa tgaaaatttc ctaaaaaagt tgttatctta
    31261 agattttttt attctttcta aagattattc cattcatttt tcacagcata aaatcgtttc
    31321 ggtgtaagcg gttaaaatag taaggagata aaaatcttgt ggtgaccgat ttactctata
    31381 gtattcaaaa gtagtttctc ggtattcgta caaaaaaaac atactaagat tagaggggaa
    31441 catcattgac gaatgatgtt ctcctctatt ttctattttt agattatagt gaagagtgaa
    31501 agaaagttcg aacaaagcct tgaggactac gagtccctat caaaaaccga acttctttac
    31561 tgtcatttta gaatcaggtt tacgtatgtt ggggccatga gcccgtgtat aggaaattgg
    31621 aatcgaaatg aagcagtaaa gttctttcaa cacttaaaaa ggaggaaaac aaatgcctta
    31681 cattcttgca ttagatgttt caatgggaca cagctacagt gtcctataca aagacgacac
    31741 ctgcttattt gaagacgaga tcgaacacac tcaaagagga tttcatgcat tactcgaaga
    31801 gatccagcac ttgcctgaac gtccattgat tgtttttgaa gcaacgggta tttattccag
    31861 accagtcgaa aagttctttc aagatcatca cttttcttac tgtttgttaa acccgttaga
    31921 agcgaaaaaa cagatggaag aaaacacctt acgcagttgg aaaacagata aatcggatgc
    31981 ccatcggctc gcacagactc atcttaaaaa tcaacgtcaa ccaaaagaag tacaaagcaa
    32041 tttttactta gagatgcgcg atttagcccg tttttatcaa gaaattgaga agaaaatcac
    32101 tcgactgcgc atggatttac acaactgcct tcagttgact tttccagagc ttgaacagtt
    32161 cttttcaaac aggctgaccc cttacgcatt gacattgatc cgtctttttc cccatccaga
    32221 ttttgtattg gcatctactc ggacaaaaat aaaaaataaa ctgatcaacg aaacacggga
    32281 aaaaatctcc gcgaatcgtg ccgagcaaaa ggcagatcaa atcatccact atgcacagtg
    32341 tgcctatcca gctgttgaaa aagacagtat tcactgtcaa aaaacgatct attacgccga
    32401 acttctccaa gatttactcg aacaaaaaaa agcactggcg acacaaatga tcaaaaaagc
    32461 cgaagcctct ccttgctttt tgttgtacca aacgttccct gggattggcg cactcacagc
    32521 cgctttattg ctcggtgaat taggagatat cacacgattt aaaacccata aacaattgaa
    32581 tgcctttgtc ggaatcgaca tccgacggta tcattcaggt aagtatactg gccaagaccg
    32641 catcaataag cgtggtaatc ccaaagcacg gaaaattatt ttcttcacca ttcgaaatat
    32701 gattcgccag caacgcgcag ctcctaatca tattgtcgat tattactaca aattaaaaaa
    32761 gcaacctatc cctaagaaag ataaggttgc cacagttgct tgtatgaaca agcttctgaa
    32821 atgtatgcac gctatggtta gggcacatac agagtacgac tatgcgtacg cggtctcagt
    32881 ggaccattaa ctaaaccact tacagtatac cacttctttg cccagatcga aaatttttta
    32941 cgaacggggc tttatttagt gtgccttgat tttgtcgctt tcgtttacga gctatttttc
    33001 ttgcaccttt tacttgacta atcgtaggaa agaggctgtg acaaaattct tgtcgcagct
    33061 ccctatttct tgatgcagga cagctttttc acaacctctc cactaaacgg tgttcaatca
    33121 gctgaccttc ggtattagct caaaaaacgg caaaatatga ggagctattt tcccgttttt
    33181 ttctctaata ctcaggtcaa accgctgatt tcacaacctc tgattcacgg tgttccagaa
    33241 gtagcttctt cggaaataag ccgaaatttt acaaaaactt gagaagcact tttcgtaaat
    33301 ttcttcttat ttcttgaagc tgaccacttc tgtcacaact tctgtttttc attgtccata
    33361 ggtttgatat tgtccttctg atgactcatt cgtaatgatt gaggcttgat tttcttcatt
    33421 tcccgttatc gtttgtccac tgccagaatc tgaagggctt agttctgtcg actcgttttt
    33481 ttgagaagaa gcagttgttt tcttgacttg ttcgttggtc tttttccatt ccgctagtcg
    33541 atcggccacc acttttttgt ccactttttc agaataagct gtaaccactt ttccattttt
    33601 caaacgtgct accttgtcgc catcatgagt caaaagcaaa acgaccaaat gtttgtcttt
    33661 gaattgatat tgtctgagag aaatcgcact aagtacgtca tcgatattga taaattgatt
    33721 ttcttctttt ccttttggca aatagatgtc caattcacct ttttcgtaat tggcagttaa
    33781 tttatattcc tcccaccaat aagttgagaa ttcttttttg aaattatcta ctgattcttt
    33841 gcttgctacg attttttctg tttctttctg agcaagatct gctgtcattt ttcctgaaaa
    33901 tgctgcggat aaaataagca gtaagaaaaa accgaataac caaaagagaa gaaacggtct
    33961 ttttttcctc atgccttcct ccactcctat ttaattttga ctattctatt atatacaaaa
    34021 aatgaaatag aaagacaaaa aagttgaaaa agatcgtaaa taattgaact tctttttcct
    34081 gaagcggtaa gaacgggaat tttcatacat tctctcttgg ctattcttta agcttcaaat
    34141 ccatttctgt ctgatacatc tttgaaccaa tgacccgatt tcttgatcgt tctttttcca
    34201 tcttgtgcta aatctactga aataaaaccg taacgattct tataggcatt ggtccacgac
    34261 cagttatcca tgcatgtcca catatgataa cctaaacaat tggcaccttc ttgaatggct
    34321 tgatggacat atttcagatg agattgaaca aattcgatcc gataatcatc gtggatcata
    34381 ccatcttcct tgataaatcg tgcttcattt tctacaccca tgccgttttc agaaataaaa
    34441 cagtcgatat tcccataatg atctcgtaca ttgatcaaaa tatcatagat tcctttttca
    34501 tagatctccc atccgcgata aggattcatt ttacgaccag gcatttcata attgtcgaag
    34561 taatcctcag gcataggacc gtttttagta tcgattggtg tttcttttgc ttttattcgg
    34621 cgtggttggt aataattaat tcccaaaaga tctactgtgt tttctttaat gatcgtcaaa
    34681 tcatctggat gaatctccgg cattagatcg agttctttta ttctctcaat caattcactg
    34741 ggaaactcgc cttttacagc tggatcaagg aatgaacgat tgaagaacgc atctgctatt
    34801 cgtgctgctt taacatcttc gggatttgat tcgtctcttg gataactcgg tgtcaaattt
    34861 aagatgattc cgatcacacc gctttgatcc atttcatggt aagctttgat ggctaatgct
    34921 gaagccaacg cttcatgata accaacttgg attgcctttt tcatatctga ttcttccgga
    34981 taatgccatc gatacaaata gcctgcctcg actggaacga ttggttcatt atgggtaaac
    35041 cattttttta ctttcgagcc aaacaattga aaacaacttt ttgcgtattt ttcataggct
    35101 tcaaccgttt ctcggttcaa ccatccgcct ttttcttgca gatacatcgg catatcgaaa
    35161 tgatacagat taacaaaggg ttcgataccg ttttcaatca actcatcaaa atagtgatga
    35221 tagaaacgaa cggcttcttc gttgattgtt ccagattcat caggtatcaa acggctccat
    35281 tggatagagg ttcgaaatga attatgccca gtctgtttca tcaactgtac atcctcttga
    35341 tacttcgtat aaacttgcga ggttttttca ggtcctactt gatcaaagaa cttttgcggt
    35401 tcgtttttgt accaataatc aaaaatattc tctccttttc catcgcctga aattcttcct
    35461 tctgtttgtg gaccacttgc tgcagagccc caccaaaaat tttttggaaa ttgatatctt
    35521 ttcatcaaag cacctctttt tcttttttag ttgattccat gtatgatatt ttccatcaca
    35581 atcacttttt cattttcttc ctcttctttt ttgattcgtt tcaacagctg ttctgctgct
    35641 cgttccccga ttttatctgt tggtggcgtg atcgtgattt ttggaaggat actcagatcg
    35701 tttttatcta aataatcata gtgactgaac gcaatatcct tcccgattct gacaccttct
    35761 tgattgaaac attcgatggc accaatcgac atgttgtaat tgctgacaaa aatagcagtt
    35821 gccccttttt ctaataatgc ttttgccccg taatagccgc tttttctaga atagtctcct
    35881 gtaaagatct ctaaatggat agacggatcc ttttgtaccg cttctttggc tccagctaaa
    35941 cgttctcttg ctacgtaatc tgtttgagcg gcagcaataa atcctattgt tttatgtcct
    36001 tgtccgccat gatgagcaat cacttttgct acactttctc gatcattcac tactattgta
    36061 tcaatacctg ctggcccacc cggacaattc aatgcgacaa ctgggatact catttttgtc
    36121 agcatttcaa atccagacca ctcttcttct gataagaaga cgatcaatcc atcgatcgca
    36181 tgcttcatca ggtattcgat tttttgttcg attttttctg gctgatcggc aaacccgctt
    36241 aataataagc tatacccatt ttcttcagct actctttcga tccctgaaac aacctccgaa
    36301 ccaaatcggt tagatatccc gtttatcaac agtccaatag agaaacttcg attattcttc
    36361 aatcctttag caatgatgtt ttctttatag tccaacgctg aaatcgcttc tgatattttt
    36421 tccatattct tttccttcag ctgctgaccg tttaaatagc gggaaactgt tcccactgaa
    36481 acaccagcca tcttagccac gtcacggatc gatgccatac attttcactc ctgccagtta
    36541 catttgattt ctttttcttg ttgttcgacc atgatgaccg ctttctttgc ataatgccgc
    36601 ttgtcatcaa tatccatata actaaagtct tcagaagtga gttcaaaaac aagcagtctt
    36661 tcctcttgag accgcaattc atctttacga aaagctttca ataatttcgt tctttggatg
    36721 acaacgccgt caattaatcg gacatagaca agagtacttg ttttacctga aacacttcct
    36781 gtatttttaa ctttaattga tattattact ttgtcaatat ccgttttgac ttccatctga
    36841 ctgtattcaa atgttgtata gcttagacca tgtccaaaag gataaaatgg acttccgctt
    36901 aaatcataat aattctcttg tttagaagca gcacgctgat agtaataaac aggtagttgc
    36961 cgactatttc taggataaga caaactcaat cgtccgcttg ggttatttct tccagaaatc
    37021 atctgcgcaa tggcttttcc accctcctgt ccaggaaacc atgctgttgc aatggcattc
    37081 gtaaatgact cgattttcat caaatcgtag ggtcttcctt gcacaagtat cgtgatgatt
    37141 ggcttgccta gttgatacaa ttgttcaatc agctgttctt ggtatccacc taaagaaaga
    37201 gaagcaacat ctacattttc tccagaatcc atgttgatcc ctttcgatga aactgccccg
    37261 ttacgaagaa attccatatc aaagttccgc gcacttgatc cacctagaac tgcaatgatc
    37321 acatctgagt tttgagcaag ggtcaccgct tctaataatt tttcttcctg atcctcctga
    37381 tttcttactt cacatccttg tgcataagaa acctttgatt ggggaaactc tgtttttatc
    37441 tcttgtacta tctttttttt caaacggtct tctgtttgtg gtgcagagta gtctcccaac
    37501 aagtggtaaa catcgtgagc atttggacct ataacagcaa tgttttttac ttgttgatcc
    37561 aacggcaata taccgtcatt ttttagtaaa gtgatacttt cttttgctgt ctctaaattt
    37621 aagtgctctg attcctccat gagatgatcc caatactcct ttggatcgtt tgcaaatggc
    37681 tgttcaaata aacctaataa aaatttgaca gataaaactc ggcgaactgc ttgatctagt
    37741 agcttttcat ctacgacttg attatcaatg gcttctgcaa tcttggtata ggtctcatcc
    37801 cacaaactga gatcgattcc tgccgctaat gcatatgctg cagcagtctt tttatctgta
    37861 aaaacatcgg ataaacgatc caaagccaca ccgtctgcca taacgattcc ttgataaccg
    37921 atctcttttc ttaatgtttg ctcaaaaagc gctcgattga tatgacaagg tatcccgtcg
    37981 atgtcattat aagcagccat gacacctact gcatttcttg tactcttcaa taatggtccg
    38041 taaatatcgg agaactctct gcgtccaatc gttactgtgc ctgaattatg accgcctaaa
    38101 gcttctcctt gtgcgataca gtgctttaac acaacaccga tctgatcctt cttcttattg
    38161 atttcctcta ctgtctgatc aagaaaagaa actttttctg aaataatctc tccttggaac
    38221 cctcgaacga ccgcttcaga aaaacgagct gagagaatcg gatcttcccc aaagcactct
    38281 tctgtccttc cccaacgagg atcttttgcc agatccaaag tcgacaccag cgctaaatga
    38341 acacctttag cagccagttc ttttgccatc aatcgactcg attgttctaa taatgctaca
    38401 ttgaaagtat ttccacggcc gatattcgtt ggataagaaa tcccatccaa gccttgatga
    38461 ccatgtggac attcttctac aagcaaaact ggaattttcc atcgcgaatg ctgtagtaca
    38521 tattcttgga ctaggttagc caccttccag ctttcttttg caggaatccc attcttataa
    38581 tctgttttag accacggatc agagcgaaac agaccgtata aggcacccag accgcctccc
    38641 catttaacat gttcttttaa cttttctgtt aattcgatct tgtctccgtt tttttcataa
    38701 cattcccaac catacaaatg ctggtttacc tgtcctactt tttcagcaat attcatcctc
    38761 gaaagtaaat cttctactct ttggcggatg gttaactttg ggttttgata cgggtaatcg
    38821 tacaccttaa tatcctcctt ctgccaattt tgccttatta gcggctaata caaatggcag
    38881 gaataaaaat acatctagaa tgataagtat tactgttaag actgctgctc gtatatcgcc
    38941 tcctgtagca ataaaagatt gtataaccgg tggagtgatc caaggtgcag cagcaactgt
    39001 tttactgatg attccaagac ttgtggctgc gtaggcaata accaagttaa tagatggtat
    39061 aatagcgcat ggaatcatat atattggatt catgactact ggtaatccaa acataattgg
    39121 ttccgtaata ttaaataaac aagtagtcag accaaattta gcgattgatc gctgttcttg
    39181 acgtttagaa gcccacaaga tagcaataac taacggtaaa atacacccgc cacctccgat
    39241 caatccaaat gcattggtaa agggctccgt aataatatta ggaatttctt ttcctactga
    39301 aaaagcatcc atgttttcct gaatagactg taacaaaggt gcttgccgta taggagccaa
    39361 aatactcgaa ccatgcatac caaacaccca taagaaatca gagaaaaact gtaaaaataa
    39421 gatgcctggg acggataaaa caaaagcttt caatggtgtc tgaatcgcta attcaattaa
    39481 ttgcggaaca ttcaaaccag tcgtatggtt taaagtaaaa acgacaactg aaaaaaataa
    39541 aatgacaact atttctggta ctaaagaatt aaatgacgca gctactgccg gtggaactcc
    39601 ttctggcatc ctgattttaa aactcttcat tttcgctaat ttgataaata attctgtact
    39661 gacaagggca gcaatcatcg ccgttgctaa gccagacgaa gaagtcagac tttgtaagaa
    39721 aacccctgta gcttctgctg tatttccgtc catcaaagtg actgtacttg ataaagacat
    39781 caaaataaac attacggctg tacttagaat tcctgcatga acaggactaa atgatggatc
    39841 atcaatcacg cctgtttgga tatagtgggt tgccaaattc cagccgatat tgtaacacac
    39901 gataatagct aaaatactca tcgtaccatt attcacgatt gaagcaagtt cagccaatgg
    39961 acttagatca agtattcttt ggactgttgc atttgaaaaa atcagagcat tcagcaaaac
    40021 catgacagaa gcagccatta ctaacggcat agtaacgata aaactgtctc gaatggccag
    40081 caagtgtcgc tggctactca tcttagatac gattggtagc attttttcag atagtctgtc
    40141 tacgactttc attatttcat ctcctagatt ctaaaattga accggttcat ttttaatata
    40201 gcactttttt taaagattgc aacacatttt tttaaaacgc tttctaaagg tggtggattt
    40261 tttatgactt ttgaaaaatt attctattta atggtatttt gtgaattttt tctcttccat
    40321 ccgtttgaag ttttactttc caatggtata atttctttat cctatcaaat gggggaagta
    40381 ttttgaagaa attaatcgta ctatttattt tgtctgcggg acttttgaca ttgactggct
    40441 gtgatttgtt tggcggctct gaccaatcct cggacgaaac aaccgattct tctgtcttaa
    40501 aagcggaaaa agcagatatc accaacagtg tatatacgct aaaagtacgt aaagatatgg
    40561 atttatccgc gggacagttg acatttacag ataaaaaaat ggaatggaca cgcacgtatc
    40621 agacacaagg agctgattct tctgaatcat ctgaagaaaa aacagtgctg acagatatca
    40681 agattactac caaagatgac gaatatatta tttcaggaaa agaaaataaa aaagaagcaa
    40741 cagtcacatt taccaaaatc ggtaattacc gtttgaagga taaggatggc aacatctaca
    40801 gtctatagtg ccatctgttg gatcttcttt tgtttctccc cttcttttct gctatagtaa
    40861 agaagcaaga tgactgtaaa ggagacgaaa tggatgatcg atagtcctga ttattgtaca
    40921 tgtaataaca acaaaaatat ccatacagaa tttaatgatc aaggaagttt gagtatatgc
    40981 gacaattgcc acaaactagt caaagattct tttaaatcgt ttgaatctta tcctgtcaaa
    41041 aattatgtaa aaagcaaaag aagataagca aaatcaagca aaactcacta tggctgattt
    41101 ttatgaactt caaactcagc ttcttccttt gatcaagctt caaaaactgg aatcagtaaa
    41161 acaaacagtg gatcatttca gttcacaaat caaagagtaa cgttttattt atttcacatc
    41221 tttgaatata aaataaccaa acgatagaaa gacttattct acatcacgta gtaatttttc
    41281 tttcccgtcg tttggttttt tacttttagc ctaattcttg acctttcgca tgctgattat
    41341 ctgtaaaata ttt
//
LOCUS       DAZABD010000017        40893 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000017 DAZABD010000000
VERSION     DAZABD010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40893
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.017"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            161..391
                     /locus_tag="ACHALG_000993"
     CDS             161..391
                     /locus_tag="ACHALG_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HFN2586419.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            488..1789
                     /gene="murA"
                     /locus_tag="ACHALG_000994"
     CDS             488..1789
                     /gene="murA"
                     /locus_tag="ACHALG_000994"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFN2586420.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1802..1978
                     /locus_tag="ACHALG_000995"
     CDS             1802..1978
                     /locus_tag="ACHALG_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFN2586421.1"
                     /translation="MSNQRYIILTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2113..2409
                     /gene="yidD"
                     /locus_tag="ACHALG_000996"
     CDS             2113..2409
                     /gene="yidD"
                     /locus_tag="ACHALG_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFN2586422.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            2593..3060
                     /locus_tag="ACHALG_000997"
     CDS             2593..3060
                     /locus_tag="ACHALG_000997"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN2586423.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3145..4029
                     /locus_tag="ACHALG_000998"
     CDS             3145..4029
                     /locus_tag="ACHALG_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFN2586424.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4143..4379
                     /locus_tag="ACHALG_000999"
     CDS             4143..4379
                     /locus_tag="ACHALG_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HFN2586425.1"
                     /translation="MMSKKNKDVEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4467..4547
                     /locus_tag="ACHALG_001000"
     tRNA            4467..4547
                     /locus_tag="ACHALG_001000"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4501..4503,aa:Tyr,seq:gta)
     gene            4554..4625
                     /locus_tag="ACHALG_001001"
     tRNA            4554..4625
                     /locus_tag="ACHALG_001001"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4586..4588,aa:Gln,seq:ttg)
     gene            4750..4920
                     /locus_tag="ACHALG_001002"
     CDS             4750..4920
                     /locus_tag="ACHALG_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2586426.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
     gene            5140..5847
                     /locus_tag="ACHALG_001003"
     CDS             5140..5847
                     /locus_tag="ACHALG_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFN2586427.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5907..6551)
                     /locus_tag="ACHALG_001004"
     CDS             complement(5907..6551)
                     /locus_tag="ACHALG_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFN2586428.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6678..6965
                     /locus_tag="ACHALG_001005"
     CDS             6678..6965
                     /locus_tag="ACHALG_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2586429.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            7073..7393
                     /locus_tag="ACHALG_001006"
     CDS             7073..7393
                     /locus_tag="ACHALG_001006"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFN2586430.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7474..8787
                     /locus_tag="ACHALG_001007"
     CDS             7474..8787
                     /locus_tag="ACHALG_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFN2586431.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            8784..9503
                     /locus_tag="ACHALG_001008"
     CDS             8784..9503
                     /locus_tag="ACHALG_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFN2586432.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            9631..10182
                     /gene="raiA"
                     /locus_tag="ACHALG_001009"
     CDS             9631..10182
                     /gene="raiA"
                     /locus_tag="ACHALG_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFN2586433.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            10404..12938
                     /gene="secA"
                     /locus_tag="ACHALG_001010"
     CDS             10404..12938
                     /gene="secA"
                     /locus_tag="ACHALG_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFN2586434.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDIQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGDE
                     DAVIQSKMLTKQVEAAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIVDFAGSTLVHEDSISLTDIEGKTSQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKDAN"
     gene            13065..14166
                     /gene="prfB"
                     /locus_tag="ACHALG_001011"
     CDS             join(13065..13136,13138..14166)
                     /gene="prfB"
                     /locus_tag="ACHALG_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFN2586435.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAVMQEEAEARI
                     NALDEKMNTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            14271..14957
                     /gene="ftsE"
                     /locus_tag="ACHALG_001012"
     CDS             14271..14957
                     /gene="ftsE"
                     /locus_tag="ACHALG_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFN2586436.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            14950..15834
                     /gene="ftsX"
                     /locus_tag="ACHALG_001013"
     CDS             14950..15834
                     /gene="ftsX"
                     /locus_tag="ACHALG_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFN2586437.1"
                     /translation="MIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFMAVI
                     MNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELKKIQ
                     NEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEKAADLKNVSRSDYGGASSDRIF
                     QIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRWPFF
                     LEGGWIGLIGAILPILIITFGYAWFYQLINPSLLRSHYSLIHPENIVWKINLLMVGIG
                     VIIGSLGSIISMRRFLKF"
     gene            15986..17434
                     /gene="cls"
                     /locus_tag="ACHALG_001014"
     CDS             15986..17434
                     /gene="cls"
                     /locus_tag="ACHALG_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFN2586438.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            17771..18628
                     /locus_tag="ACHALG_001015"
     CDS             17771..18628
                     /locus_tag="ACHALG_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFN2586439.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            18743..19663
                     /gene="pstC"
                     /locus_tag="ACHALG_001016"
     CDS             18743..19663
                     /gene="pstC"
                     /locus_tag="ACHALG_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HFN2586440.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            19663..20547
                     /gene="pstA"
                     /locus_tag="ACHALG_001017"
     CDS             19663..20547
                     /gene="pstA"
                     /locus_tag="ACHALG_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFN2586441.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            20562..21368
                     /gene="pstB"
                     /locus_tag="ACHALG_001018"
     CDS             20562..21368
                     /gene="pstB"
                     /locus_tag="ACHALG_001018"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFN2586442.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            21384..22142
                     /gene="pstB"
                     /locus_tag="ACHALG_001019"
     CDS             21384..22142
                     /gene="pstB"
                     /locus_tag="ACHALG_001019"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFN2586443.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            22155..22832
                     /gene="phoU"
                     /locus_tag="ACHALG_001020"
     CDS             22155..22832
                     /gene="phoU"
                     /locus_tag="ACHALG_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFN2586444.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            23409..24101
                     /locus_tag="ACHALG_001021"
     CDS             23409..24101
                     /locus_tag="ACHALG_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFN2586445.1"
                     /translation="MLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISIL
                     FVVIGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYL
                     TRPYRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVI
                     GSMTNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPIS
                     VLFFLTVRRLKR"
     gene            24098..24865
                     /locus_tag="ACHALG_001022"
     CDS             24098..24865
                     /locus_tag="ACHALG_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFN2586446.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            24965..26533
                     /gene="liaX"
                     /locus_tag="ACHALG_001023"
     CDS             24965..26533
                     /gene="liaX"
                     /locus_tag="ACHALG_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HFN2586447.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            26830..27150
                     /locus_tag="ACHALG_001024"
     CDS             26830..27150
                     /locus_tag="ACHALG_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HFN2586448.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            27150..27500
                     /locus_tag="ACHALG_001025"
     CDS             27150..27500
                     /locus_tag="ACHALG_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFN2586449.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            27667..28605
                     /gene="hprK"
                     /locus_tag="ACHALG_001026"
     CDS             27667..28605
                     /gene="hprK"
                     /locus_tag="ACHALG_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFN2586450.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            28620..29450
                     /gene="lgt"
                     /locus_tag="ACHALG_001027"
     CDS             28620..29450
                     /gene="lgt"
                     /locus_tag="ACHALG_001027"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFN2586451.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            29494..30519
                     /locus_tag="ACHALG_001028"
     CDS             29494..30519
                     /locus_tag="ACHALG_001028"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFN2586452.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            30534..31472
                     /gene="galU"
                     /locus_tag="ACHALG_001029"
     CDS             30534..31472
                     /gene="galU"
                     /locus_tag="ACHALG_001029"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFN2586453.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            31597..32037
                     /locus_tag="ACHALG_001030"
     CDS             31597..32037
                     /locus_tag="ACHALG_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HFN2586454.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            32040..32642
                     /locus_tag="ACHALG_001031"
     CDS             32040..32642
                     /locus_tag="ACHALG_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFN2586455.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(32701..33807)
                     /locus_tag="ACHALG_001032"
     CDS             complement(32701..33807)
                     /locus_tag="ACHALG_001032"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFN2586456.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            34017..35018
                     /gene="ccpA"
                     /locus_tag="ACHALG_001033"
     CDS             34017..35018
                     /gene="ccpA"
                     /locus_tag="ACHALG_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HFN2586457.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(35355..37700)
                     /locus_tag="ACHALG_001034"
     CDS             complement(35355..37700)
                     /locus_tag="ACHALG_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HFN2586458.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGEA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            37979..38881
                     /locus_tag="ACHALG_001035"
     CDS             37979..38881
                     /locus_tag="ACHALG_001035"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFN2586459.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAEKGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            39094..40869
                     /locus_tag="ACHALG_001036"
     CDS             39094..40869
                     /locus_tag="ACHALG_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HFN2586460.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
BASE COUNT        13311 a         7120 c         8731 g        11731 t
ORIGIN      
        1 tttgtctcag tccctttttt tgcatatttt tagcatttat ttcctaaaaa atgtaagggg
       61 attcctatat ttttaagtat acttgttttt ccttttatac attttatggt attatcattt
      121 tattagttat tataaaacaa tcgttagaaa ggacgtcatt atgcaagtat acggaattga
      181 tgcggtgctt cgaatcgtga gtcatttggc gtttatctac ttagccttct ggtctttacg
      241 ttcgttacgc attgaaactt ttttcagacc aatgcataat acgcaaatta gaatggcaat
      301 cgtacttttt tcaatttttt tagggtacac tgcaagtaat tttttcttag aagttatcgc
      361 tttatgccga aatcttttcg ttagcctgta aattttaaaa aaaggtacta ttttaaaagc
      421 aaggatgtta taatagaaaa gattttgagt aagtgtatgc tcattggaat tttttggagg
      481 gaacaacatg gaagagatca tcgtaagagg tggcaatcaa ttaaatggaa cagtaagaat
      541 tgaaggagct aaaaatgctg ttttgcctat tttagctgct agtttgttag cagaagaagg
      601 tattacgaca ttagacaacg ttccgattct ttctgatgtc ttcactatga atcaagtgat
      661 tcgtcactta aacgtagatg tagattttga tgaacaaaag aatcaagtaa caatcgatgc
      721 ttcaagacaa ttagaaatcg aagcgcctta tgagtatgtt agtcaaatgc gtgcttcaat
      781 cgttgtgatg ggtccgctac ttgccagaaa tggacatgca aaagtagcta tgcctggtgg
      841 atgtgcgatt ggtaaacgac caatcgattt gcatttaaaa ggatttcaag ctttaggagc
      901 aaaaattatt caaaaaaacg gctatatcga agcaattgcg gatgaattga tcggtaacac
      961 gatttattta gatttcccta gtgtaggtgc tactcaaaat atcatgatgg ctgccgtaaa
     1021 agcaaaagga acaaccatca ttgaaaacgt agctcgcgaa ccagaaatcg ttgatttagc
     1081 aaacattttg aataaaatgg gcgctcaagt ctatggagct ggtactgaaa caatgcgtat
     1141 tgaaggcgtg gatcatttac atgcagtcaa ccactcaatc gtacaagatc gtatcgaagc
     1201 aggtaccttt atggttgctg ctgcaatgac acaaggcaat gtgctgattg cagatgctat
     1261 ctctgaacat aaccgcccat tgatttctaa attgatcgaa atgggtgcag aaatcattga
     1321 agaagaaggc ggagtccgtg tcatcggacc aaaacatatc ttacctacag atgtaaaaac
     1381 gatgcctcat cctggtttcc ctacagatat gcaagctcaa atgacagcta tccaattagt
     1441 tgcagaaggt acaagtgtag tgactgaaac agtgtttgaa aatcgtttcc aacatttaga
     1501 agagatgcgc cgtatgaacg ctcatgtcaa aatcgacgga aatgtagcaa tcatggatgg
     1561 aaaccatgaa ttgcaaggtg cagaagtata tgcaacagac ttgcgggcag cagcagcatt
     1621 agttttagct ggcttgaaag caaatggaat cacacgtgtg cgtaacttga attatctaga
     1681 ccgtggatat tataatttcc atatcaaact acagcaatta ggtgcagacg ttgaacgtgt
     1741 agacatggat caaacttctg ctgaaaaaac agcacaaacg attgcttaag gaagtggctg
     1801 aatgagtaac caacgatata tcatattaac tttgataaag attttagtgg tgatcttatt
     1861 gcttatcctt ctttttgtgg ccggaacgat gatcggatat ggtgtcatcg gtggcggcaa
     1921 tccatttaaa gtattccagc caagtttgtg gattcatatt cgagactttt ttcattgata
     1981 aataagaatc acagtggttc tagtcgatat gaagtgagcc atgaagcaga acatgacaaa
     2041 agaaaacaga agggaacgtt gacgttagtc gcgatccctt tttttagtaa aagaaaagaa
     2101 ggaatctaat tgatgaagaa gatattgatt tatttagtta gaggatatca gcgctttatt
     2161 tcgcctgcat ttccacctag ctgccgttat tacccaacct gttccaatta tatgataaaa
     2221 gcaattcaaa cccatggtgc actaaaaggg acggtaatgg ggactgcacg gatcttacgc
     2281 tgtcatcctt ttgtcaaagg cggtatcgat tatgtaccat tgacattcca attaacgcga
     2341 aataagaaag ataaaaaaga accagattat aaaaaggttg tgaaaaatac gagtagcact
     2401 gagcgataaa gaaacaattt ggaaaatagc ttcttatatt ttgcaaaatt gtgagttatt
     2461 gccgaagtgt tggattttga acaccgtcaa aaaaaggttg tgaaaaatac gggtagcact
     2521 gagcgataaa gaaaaagagg aaaattattg tcaaagtaac aactccgcaa ctacttatat
     2581 gggggacaaa aaatgatcga agaactaaac aagaaagatt taccatggtc cttgttatta
     2641 gatgcagatc ctgataaaga aaaagttcaa gtatatgttt ccagagggag tggctggata
     2701 tggaaagaaa aagaaaaaac aataggtgta ctgatctatg tggcaaggga aaaagaatac
     2761 gaaatcgtca atgtagcagt agccccaacg tatcaaggaa aggggattgg cggaaaatta
     2821 cttgagacag cttttcagaa gctttcacaa ctcgcttcca gtcaaactca gatcatcata
     2881 cgaaccggca gtactagcag tgcggcgtta catctctatc aaaaaatggg ctatgtggag
     2941 attggtcgag tcaaagacta ttttatccat cattactctg agcccatttt tgaaaatgga
     3001 gatcaattaa gggatcaagt aattttagca attcacttac ctaaaaatag ctttaactaa
     3061 taaactttca attcgagaaa atgctttcaa atgctaaata gatacattac aatagcttta
     3121 gcaaacaaaa aggagaatat ttttatggga atattagttt ttgattttgg tggatcggct
     3181 gtcaaatatg gttgttggga tggcaaagag attaaaggta agggacaatt tgcaacgcct
     3241 gaatcttggg aagaaatgaa agcgcaatta tttcaagttt ataaaaaaat actcgtttct
     3301 tttgaagtcg aaggaattgg aatcagctct cctggagtag tgaataatga gaaacaggtg
     3361 attgagggaa tcagtgcgat tccttatatt catggattta atattttccg agatttggaa
     3421 actctgtttc aactcccagt aacgattgaa aatgacgcca actgtgccgg aatggctgaa
     3481 ttttatgaag gtgctgcccg tgattatcaa gatgttgctt ttgttgtggc aggaactggg
     3541 attggcgggg cgttgttcac gaaaggacaa atcaataaag gggcacatct ttatggtgga
     3601 gaatttgggt tgatgttctt aaatggcgat aagacattca gtaaacttgg tacagcggta
     3661 cagatggctt ggcgttattg tgaacgcaaa ggtctcgata aaaattctta taccggaaaa
     3721 gatgtctttg aattagcaga aactggagat gcaatagcaa aagaagaagt tgagagtttt
     3781 tatacgtatc ttacaaaggg attattcagt attcaatttt cgtttgatcc agaagtgatc
     3841 gtattaggcg gcggtgtttc tgcaaaagaa gggctcattg atgagatcaa aagccgaatg
     3901 aagaagctga cagaacaatt cgatctgcat gatttcgaac cgcagatttt attatgtgaa
     3961 tacagaaacg atgctaatct tgtaggtgca gcagcaaact atctcgctgt acgtcaaggg
     4021 gagaattaag agctttagag aaggtgcagc ttgcataaaa gaagtgaatg aaatgaatag
     4081 gagattttta cctaaggaat gttttatttg ctgcagtatg ataaaatatc tgagaggtga
     4141 aaatgatgtc aaaaaaaaat aaagatgttg aagtgcgtat agaagaagtg aaaaaagata
     4201 tcaaaggtaa gaattatgaa gtcactcagt tatttatagg gaaaaaaatg attggtgaga
     4261 tcttagccta tgggccaaaa gaatatgaaa ttttctttgg tgaagaagat tttggaaaag
     4321 aaaaaagtct tgaaaatgcg atagaaacag tgattcgaca ctggaatctg cacgaataaa
     4381 aaaatatcaa aaaaactttc aataaagggt tgcaaaaatc tggaaaatta ggtattataa
     4441 ctaagtcagg ttgatacaaa cctttcggag gagtagcgaa gtggctaaac gcgacggact
     4501 gtaaatccgt tccttagggt tcagtggttc gaatccactc tcctccattt cattggggta
     4561 tagccaagcg gtaaggcaag ggactttgac tccctcatgc gttggttcga atccagctac
     4621 cccagtatac cacaatcgta agctctcgtt agttcgagag ctcttttttt tgctttgcta
     4681 ccttgttcct tgaagattct gctgatttaa gatagagtta agttaactac aagaattgga
     4741 gggatccaca tgaaatggaa agaaaaagta gacgaagcag ctgaaaagtt atatgatttg
     4801 gtcaaatcag aaaaatacaa tattgaagtg aatattccga aaaaaggcgg aaaagcggtc
     4861 caggtaaaat caaaacgccc aacgaatcat acaaaaaaat ggatggctaa aaaccgctaa
     4921 taataacaaa agatccataa gtcggcattt tcatcgattt tagggtcttt tgttattttc
     4981 taggttgaac atatctaaaa aattgtttct gacaatagga gtaggaagaa cgatgtgtgt
     5041 ctaaatcctc tgttgatgga tagaaacctt tatctttttc agttgtgaat caggtatata
     5101 ccagttataa tagaagaaac gaaaggacgg gtgtacgtta tgtcgaatca attacctgta
     5161 tatatacaga ttcatgacca attaaaaaat gaaatcgaac aaggtatatg gaaagtcgga
     5221 gaccgtcttc cttcagaaag agaattagca gtgaaattca atgtcagccg tatgacgttg
     5281 agacaagcaa ttcaaaactt ggcagatgaa ggaatcttag aaagaaagat cggttcgggt
     5341 acatatgttg cgcgagaaaa agtacaagaa aaaatgtctg gtgcaacaag ttttacagaa
     5401 attatggaat cgcaaaatcg agtaccgtct agtcggacaa tctcttattt cttgacttcg
     5461 ccaagctcaa gtgaaatgga aaaactaaac ttgagtaaag atgacacgat cttgcgaatg
     5521 gaacgtattc gtttcgcaga tgatgtccct atttgttttg aagttgccag tgtgccacaa
     5581 aagctgatcg atggctacag caaagcagag attactgaat cgttgtatcg cacgctagaa
     5641 gaaaaaggcg ggaaaaagat cggtccagcg aatcaaacga ttactgctgt gttggcttct
     5701 gagaaaattg ctgactattt agaggtaaaa cgaggagatg cagttcttcg tttgcgccag
     5761 atttcttatt ttgaagacgg tacgccgttt gagtatgtgc gtacgcaata cgtaggaaat
     5821 cgttttgagt tttatttgga aaaataaaaa acaggtgatc cctgattcct tacttagaaa
     5881 taaaagagaa tcagaatcac ctgtttttat atatgatcag ttaccagttg ctcgtgataa
     5941 gaaacaggac cctcttcaaa ttggacttgt ccgttcagca gctcaatgat ggattcttta
     6001 aagtggtcag cacttttttc atctacagca cagatgacgc taacttgatc agtatattgg
     6061 atttctttta taaaaaaatg ttggagttca agataattct gtaattttcc tagttgcgga
     6121 tacgtgatca tcacagagat ctcctgttgc aaggtacctt ctacgattcc aatgtcagac
     6181 aatgcttgag agacagcatg agtgtatgct cgtattaatc cgcctgtgcc gagtttcgtt
     6241 cctccaaaat atcgagtgac tactgcagct acattgatca gttgattttt tttgaggact
     6301 tccaacatcg gtacgccagc cgttccactt ggttctccat catcgctgct tcgctggatt
     6361 tcattttttt ctccaatgat gaaggcgctg cagttatggt tcgctttcca atgttctttt
     6421 tttatttctt gaatgaaact ttttgcttgg tcctctgatt ctattcgtct aagagaacag
     6481 ataaagcgtg attttttgat ctcaatctca ctttgtccat tttcttttat tgtacgatag
     6541 cgctcgatca tcttgtcacc tctttttttc attttcttta ttcagtataa ataaagttct
     6601 aggttgtttc aacactgtaa cggttgcgca atcgctgtag tatgcgataa taaagacaaa
     6661 ggtaggtgga tctggggatg caggaacgaa tgaaaaagta cgatgcaatt acgcattatt
     6721 tgaagaacaa tggcggctct caagtaacgt tgacctttac ccaatttgac gaactgctgt
     6781 ttcccagtaa cggacttccc aaaacagcaa gggaaagtac agattggtgg gcaaatgatt
     6841 ataaacaccc tgaaaaaggt gcctatggct ggataaatgc aggctatgaa gtagttgtca
     6901 tcaatttgga caaagaatat gtcgtgttca ataaattagt gaaatctagt tggctatttg
     6961 attaaataaa ccgcgtttag aaaacgttga caaatttctt tctttttata taatagagta
     7021 acagacgcga agaaaacgtt tgcgtagttt gttttaaaaa ggagagaaaa gtatggctaa
     7081 aaaaacaatc atgcttgtat gttcggctgg tatgagcaca agtttactag taacaaaaat
     7141 gcaaaaagct gctgaagcaa aaggaatcga ctcggatatt ttcgctgtgt ccgcttctga
     7201 cgcagataac aatcttgcaa ataaagatgt agatgtactg ttattaggac ctcaagttcg
     7261 ctttatgaaa gctgattttg aaaaacgtct ggaaccaaaa ggcattcctt tagacgtcat
     7321 caatatggct gattatggta tgatgaatgg cgaaaaagtg ttagatcaag caatcacgct
     7381 cataggtgaa taaatagaac agtataacta tcgaacaagc gatccaaaaa atagatcgct
     7441 tgttttttcg tatatttttt tagggcgaat aatatgaatc atttttgggg tagaaggctt
     7501 ctgacgagag atattaaagc aatagactgt gaggaggtaa atttaccaaa ttatgagaag
     7561 atcgcagcag tggaacgttt gggcaactgg attctttgtc atcgctgtgg aatgaggacg
     7621 tccgtcattg atggacagct tgcagattac ggttattttt gtacacattg tctatattta
     7681 gggcgatgcg acactcaaca agatttatat ttgtttgatc agcctgaagc aaaacctcga
     7741 gaagtcgtct ttttttggac aggacagctt actgaaaaac aaacggaaat cgcaaagaga
     7801 atactttgcc attcagaaaa aagacatcat cttatttggg ctgtaacagg tgcagggaaa
     7861 acagagatgc tttatcctat tttagtggaa actttaaaag ctggaggaag agtagcgatt
     7921 tgtacaccgc gagtcgatgt ttgtaatgaa ttgtttttac gctatcgtta tgtttttcca
     7981 gaagaaaaaa tcacgttgct ccatgggaat acagcaacgg cttatacctt tacttctttt
     8041 gttatctgta cgattcatca gctataccgt ttttatcggt cctttgattt aatagtgatc
     8101 gatgagatcg atgcgtttcc ttataacggg gatgcaggat tagctcatgc tgtgcaaaca
     8161 gcgattaaac cagacggacg attcatctac ttaagtgcca cacctgataa aaagttacta
     8221 aaggaaatca agcagacgtt tgacatccac aaacttgctt tgcgctttca tcggcgttta
     8281 cttcctgaac ccgtcttggt tttcatgaat aattggcgac aaaaatgcaa gaacaagaag
     8341 aagataaaat ctttggtgcg gatcattcat caattaatta aagaaaacca tatcctttta
     8401 ttttgtccaa gtatcacgat gatgcatcgt ttgaaagcca cacttcaaga ggctcttccg
     8461 agatatgaga tcacagatgt ttcttcaaga gacaatcagc gggcggaaaa agtccagaaa
     8521 atgcgagaag caaaatatga catactgtta accacgatga tccttgaacg tggagtcaca
     8581 tttgaaagaa tatccgttat cgttttagga gcggatcatc cggtttttac taaatcttct
     8641 ttggtacaaa tcgcaggaag agccgatcgt aaagggcctt atacgaacag tcaagtatac
     8701 tttttctatg aagaaaaaac tgcagctatt cgaaaagctt gtcaggaaat caaagaaatg
     8761 aatgaagaag ggaagaagat gctgtgaggt gcagttgctg cagtaaagat atcgtccaaa
     8821 atttaacatt cagcgagatt attggtgttt cgaaatacag attacctcag acttgttcag
     8881 tttgtcgcgc acaatttcat ttattggaaa caagaggctg ttctggctgt caaaaaccaa
     8941 tccatttcaa tctagagagt acgaaaaaga aagtaaagtc tattcccgtt tactgcagtg
     9001 actgtcaaga atggaagaag aaatatccga cctatgattt tcagcaccgt gccttttttg
     9061 tgtacgatga aaatatgaag gcctggctca atcaatacaa gtttatggga gatatccgac
     9121 tagcaggcac ctttgcatat aaatggacgc agataaaaaa agaatacccg acatacatct
     9181 attgtccaat acctttatct gaaaaaaggc gaacagaaag aggattcaat caagtaagcg
     9241 agatgttgaa agcagcaaga attccttttc agatgctact caaaagagag aagcatctgc
     9301 tggctcaagc aaaaaacacg cgaaaggaaa gattggagat gcctcaacct tttgaacttg
     9361 cagtcacctt ttctaagata caaaataaaa aaatattgat tattgatgat gtctatacaa
     9421 caggacagac tcttttccac gcagcagatt gtttgcaggt cgcacagccg aaaatcatcc
     9481 gaaccatttc tttagcaaga tgaattagat gtggaaattt gatagcgatt taatttgcaa
     9541 ataacctaga gttcgatata atagaattaa gaagagggaa agagtggtcc ttctccttct
     9601 tgattgtggt agacgaaagg ggtaattttt atgtttagat ataatgtacg cggcgaaaat
     9661 atcgaagtaa ctgaagcgat tcgcgactac gttgagaaaa aagtaggcaa actagaacgc
     9721 tatttcagtg acgcacccga tgcaacggcg catgtgaact taaaagttta tacagaaaaa
     9781 acagcaaaag tggaagtaac gatcccatta ccttatcttg tattacgtgc cgaagaaact
     9841 tctccagatt tatacgcaag tatcgatttg gttgttgaca aattggaacg ccaaatccgc
     9901 aaattcaaaa caaaaattaa tcgtaaatcc cgtgaaacgg gcatgaatac tgcagatgct
     9961 actgctttat tcaatgaaga ggacgaaaac gaaaatgatt ctgaattaga tatcgtacgt
    10021 acgaaacgcc tttcattgaa accaatggat agtgaagaag cagtcttaca aatgaacatg
    10081 ctgggacata acttctttat ttttgaagat gcagaaacaa atggtacaag tatcgtttac
    10141 cgccgtaaag atggaaaata cggactgatt gaaacagact gatcagcctt acttaaataa
    10201 caacgaaaac ttgcagcagc tactgacagc tgccgcaagt ttttttgagt ttcctcctct
    10261 tttagatgaa atatgaataa attatgaagt tcttacttta atacgcttaa gatatttcaa
    10321 ttatctataa ctaatgataa aatgaaaagt aaagtctaca tagtctggat aatacacaga
    10381 aggaaaagga gattacacaa cgcatggcca atttgattcg ttcacttatc gaaaacgata
    10441 agaaagaact aaaacgttta gatagcattg caaaaaaagt tgaagcatac gctgaccaaa
    10501 tggcagcatt aactgatgaa caattacagg caaaaactgc tgaattcaag ggcagatacc
    10561 aaaaaggaga aacattagat caattacttc cggaagcttt tgcagtcgtc cgagaagcag
    10621 caaaacgtgt attggggtta tatccatacc acgtacaatt aatgggtggg atcgtgcttc
    10681 acgatgggaa tatccctgaa atgcgtactg gtgaaggtaa aaccttgact gcaacgatgc
    10741 cggtgtatct gaatgcgttg actggcgaag gggtccacgt agttaccgtc aatgaatatt
    10801 tagcaacccg tgactcgacg gaaatgggcg agctttataa tttcttagga ttgacagttg
    10861 ggttgaatat caactcaaaa tcttcggatg aaaaacgcga ggcttataat tgcgatatca
    10921 cttatagcac gaataatgaa ttaggatttg actatttgag agacaacatg gtcgtttacc
    10981 gtcatcaaat ggtacaacgt ccgctgaatt atgccattgt cgatgaagtt gactcgatct
    11041 tgattgatga agcgagaacg ccgctgatca tttcagggca agcagaaaaa tcaacagctt
    11101 tatatacaag agcagacaac ttcgtgaaac gtttgaaaga agaagaagat tataaaatcg
    11161 atatacaatc aaaaacaatc agtttaactg aagcagggat cgaaaaagct gaagaaacat
    11221 tcggtttgga caacttatat gacattgaaa atactgcatt gacccatcac ttggatcaag
    11281 cattgagagc caactacatc atgcttttag acatcgatta tgttgttcaa gatggaaaag
    11341 tattgattgt tgaccaattt acgggtcgga tcatggatgg acgccgttat tcagatggtc
    11401 ttcatcaagc gatcgaagct aaagaaggcg tagagatcga agacgaaacg aaaacgatgg
    11461 cgacgatcac tttccagaac tatttccgta tgtacaagaa acttgcgggg atgactggta
    11521 cagctaagac agaagaagaa gagttcagag agatttataa tattcaagta taccagattc
    11581 cgacaaaccg tccggtcatc cgtgacgatc gaccagattt gctttaccca acattacaaa
    11641 gcaagttcca tgctgtcgta caagatatca aagatcgcta ccgcaaagga cagcctgtct
    11701 tagtcggaac tgttgcagtt gaaacatctg agttattatc agatatgctg aatcaagaac
    11761 gtattcctca tgaagtactg aatgcgaaaa atcactttaa agaagcagaa atcatcatga
    11821 atgcgggtca aaaaggtgca gtgacgattg ctaccaacat ggctggtcgg ggaacagata
    11881 tcaagctagg gcttggcgta agagaacttg gtggcctagc agttatcggg acagaacgac
    11941 atgaatctcg tcgtatcgat aaccagcttc gaggacgtgc aggacgtcaa ggagaccctg
    12001 gtatgtcaca attctacttg tctttagaag atgatttgat gaaacgattc ggttcagaac
    12061 gaatcaaagc tttcttagat cgaatgaaga tcggtgacga agatgctgtg atccaaagca
    12121 agatgctgac gaaacaagta gaagctgcac aaaaacgcgt agaaggaaac aactacgaca
    12181 cacgtaagaa cgtcttgcaa tacgatgacg tgatgcgtga gcaacgtgaa gtaatctata
    12241 aacagcgtca agaagtgatc atggaagaaa aaagcttatc taaaccactg atgaatatgg
    12301 tcaaacggac aatcagtcgt gtggtagatg cgcatacgca attagaaaaa gaaaattgga
    12361 atttggaaag tatcgtcgat tttgctggca gcacgcttgt ccatgaagac agtatttccc
    12421 ttacagatat cgaaggaaaa acctctcaag aaatcaaaga atatctaaac gaacgagcac
    12481 aagaagtatt caataccaaa gcagctcaat tgaacggacc agagcaactg cttgaatttg
    12541 aaaaagtcgt gatcttacgt gtcgtggact ctaaatggac agatcatatt gatgcaatgg
    12601 accaattacg tcagtcaatc ggcttgcgtg cctatggtca aaacaatcca ttagtcgaat
    12661 accaaacaga aggttataag atgttcgaag atatggtagg tgccattgaa tatgaagtca
    12721 ctcgtatctt catgaaagct gagatccgtc aaaacgtaca acgtgagcaa gtttctcaag
    12781 gaacagcttc tcactcagaa gatggagatg ccaaagaaaa cagtaatact agcgcgaaaa
    12841 aacaaccaat ccatgtgaac aaggttggta gaaatgatcc ttgtccttgt ggaagcggga
    12901 aaaaatataa gaactgtcac ggaaaagatg caaactaatt tctgacagca ggattggtga
    12961 gacaaattct tttttaaaga atttgtctca ccttctttct gttatttgac gattctgtta
    13021 gactagatat aataaaaaaa gtatagtgaa agaaggaaaa aaacatggaa atatccgaaa
    13081 ttcgaaatct cctgacagaa atgcaggaaa aggtcacaag cttcaggagg tctctttgac
    13141 ttagaccagc tggaagaaga cattgcggaa gcagaagcaa aaatggctga acctggtttt
    13201 tgggatgata gcgaagctgc tcaaaaagtg atcagtgaaa acaatgcaaa taaagaaact
    13261 tatgatcaat tccattctct tgctgaagaa ctggaagaaa tcgaattgat ggtggagatg
    13321 ttgcaggaag aacctgatgc agtaatgcaa gaagaagcag aagcacgtat caatgcgcta
    13381 gatgaaaaaa tgaatacgta tgaattatct atgttgttag atggtcctta tgatcgaaac
    13441 aacgccatcg tcgaattgca tccaggggct ggcggaacgg agtcccaaga ctggggcagc
    13501 atgcttttac gcatgtatac acgctgggca gagcagcatg ggtatcaagt agagacacta
    13561 gattatcaag caggcgatga agcaggaatc aagagtgtga ccctactgat caaaggacac
    13621 aacgcatttg gctatctgaa atcagaaaaa ggtgttcatc gccttgtacg tatctcgcct
    13681 tttgactcag caaaacgtcg tcatacttct ttttgttcgg tagatgtgat gccagagctt
    13741 gatgaaacga tcgatataga aatcaatcca gatgatttga agatcgacac atatcgggcg
    13801 agtggtgcag gtggtcagca catcaataag acagaatccg ctgtacgaat cactcacttg
    13861 cctacaggaa cagtagtagc gagtcaggca caacgttcac aattgaaaaa ccgggaacaa
    13921 gcgatgggga tgctgaaagc taagctttac caattagaag tggaaaagaa agaacaagaa
    13981 gcagctgcac tgagaggaga acaaaaagaa atcggctggg gatcacagat ccgttcttat
    14041 gttttccatc catattctat ggtaaaagac catcggacaa acgtagagac aggaaatgtc
    14101 caagctgtta tggacggaga tcttgattta tttattgacg gctatttgaa aatgcatctg
    14161 caataagagc cctttgttaa attgaaataa aattgtaaga ttttgcaagc aagttgaaac
    14221 attgccatgc tataatagct aaagactgaa aaagatatgg agagaacgcc atgattgaaa
    14281 tgaaggatgt aatgaagaag tactcaaacg gtaccactgc tatccgcaat ctctcgattg
    14341 tcatcgaaca aggagagttt gtgtatgttg ttggtccgtc tggagctggt aaatctacat
    14401 tcatcaaatt gatgtatcga gaagaaaaag cttcaaaagg aactttgaac gtcgcaggtc
    14461 atgatctaat agccatcaaa aaccgcgaag ttccttatct acgcagagaa atcggcgtgg
    14521 tgttccaaga ctataaatta ttaccaagaa aaacagtata tgaaaatgtt gcgtacgcca
    14581 tgcaagttat cggcagaaaa cctcgggata tcaaaaaacg agtaatggaa gtgctggatc
    14641 ttgttggttt gaaacacaaa gtccgagtct ttcctagcga attatctggt ggggaacaac
    14701 aacgtgtttc tattgcccga gcaatcgtaa atacacctaa agtacttatc gcggatgaac
    14761 cgacagggaa tctagatccg gaaaactcat gggaaatcat gaaactatta gatcggatca
    14821 atgcacaagg aacaactgtc gtgatggcta cacataacag tacgatcgta aatacgattc
    14881 gacaccgagt aattgctatc gaaaacggcc gcattattcg agaccaagcg gaaggggaat
    14941 atggatacga tgattagaac ctttttccgc catttattag aaagtatcaa gagtttgaaa
    15001 cgtaacggct ggatgacagt agcttcggtc agtgcggtaa cgatcacgtt gactttagtt
    15061 ggtgtgttca tggcggtcat catgaacgca accaaactag cacaagatat agaaggaaac
    15121 gtggatgtat ctgtgttcgt agacattggt acaaaagacg aagagatgaa aaaattagaa
    15181 aaagaattaa atgacttaga tcatgtcaaa aaagtcgaat tttcaagcaa agaacaggaa
    15241 ttgaagaaaa tccaaaacga aatgggcgat tcttggaaat tgtttgaagg ggataataac
    15301 ccgttatatg atgtatacgt agtaagtgct accgatcctt cttatacaaa acaagtagca
    15361 gaaaaagcgg cagatttgaa gaatgtttcc cgttctgatt acggtggggc ttcctcagat
    15421 cggattttcc aaattgctgg tgcagttcga acatggggat tagttgcggc ggcattgctg
    15481 ctgtttgtag caatgtttct catttctaac acgattcgga tcacgatttt atctagacag
    15541 cgtgaaattc aaatcatgcg tttagtaggg gcaaaaaatg gatatatccg ctggccattt
    15601 ttccttgaag gtggttggat cggcttaatc ggtgccattc ttccgatact gatcattaca
    15661 tttggttacg catggtttta ccaattgatc aatccgtcat tattacgatc tcattattca
    15721 ttgatccatc cagaaaatat cgtttggaaa atcaatcttt tgatggtcgg tattggtgtg
    15781 atcattggtt cactcggctc gatcatctct atgagacgtt tcttgaaatt ctagtgtcac
    15841 aagataaata taatcagaaa gttggacgat ttcgttccag ctttcttttt ttatcagcta
    15901 ggtggagtaa atggggcggc ttttttctct ttactttaaa aaacaacttg ggtattctta
    15961 agttggtaga aaaagaggtg aagccgtgaa gattatcgtt gacaattttt ttacgatcct
    16021 tttgattatg aatattttgc tttcctttat tatcgttttt cgagaaagaa aggaaactgc
    16081 gcaaacatgg gcatggctgc tcgttttgat gttcattcca gttgtaggat ttattttata
    16141 tattttttta ggaagaggga tttccaaaga gaaaatcttt gatttgaaga tccaagggaa
    16201 aatcggtaag aacctggaaa ttgaagaaga caaacaggca ttgatgcgag ggctttatcc
    16261 tcatccgcct actggacaag tagatgtgaa acaattgatc tatatgctca cggtatttga
    16321 aagtacgctc tatacgacta aaaatgaaat cactttattt acagatggtc gtgaaaaatt
    16381 cgatgcgctg attgaagata tcaagcaggc agaagaccat atccacttcc agtattatat
    16441 ttatcgcagt gatgcattag gtgaagaagt tcgagatgca ttgacagagg cggcaagacg
    16501 aggggtaaaa gtcagagtcc ttcttgatgc atggggttcc acacaagttt cttccagctt
    16561 ttttcagaat ttgaaaaaag caggcggaga agtcgcattt ttctttcctc tatttgttcc
    16621 gtatattaat cctcggatca attatcgcaa tcatcgtaaa atcgtagtta ttgatgggaa
    16681 aatcggatat acaggtgggt tcaatgttgg aaatgaatat ctaggattgg taaagaaatt
    16741 tggttattgg cgagacaatc atttgcgtat ttacggagaa gcggtgtata ccttgcaaaa
    16801 ccgttttttg atggactgga attctcagca tacaaaagaa gtaggctatt ctcccaaatt
    16861 ttttccgtcc attcactcta ctggtgatat agctgttcaa atcgtgacaa gcggaccaga
    16921 taccgaacat gaacaaatca aaatgactta tttgaaaatg atttcgttag ccaaacggga
    16981 gatattgatc caaacgcctt attacatacc ggatggatcg attcatgaag cactgaagtt
    17041 ggctcttctt tcaggcgtaa aagttcatat ccaaatacct aacaaaccag atcatcttct
    17101 cgtttactgg gcaacgtatt ctttcgctgc agagctaatc gagtatggag caaggatcga
    17161 aacatatgag aatggattta ttcatgcaaa gacgatgatt atcgatggtg gcattgtttc
    17221 agttggctca gcaaatatcg atgttcgttc atttcgcttg gactttgagg tcaatacact
    17281 ggtttatgat gaacgaatgg cttcacgtgt aagaaaagct ttttttgaag actcgaaaat
    17341 ttctacgcat ctcacaaaag aaatgtatga aaaccgagga ataatcatca agatgaaaga
    17401 aggattagca agattgattt cgccgctcct ttaaaacata aaaaggacat gagaaccatc
    17461 aacagatgga gatctcgtgt ccttttctaa cggggccatt tctgagagta cgtcagaaat
    17521 ggagaaagcg tctcgcatga gcgaaattaa ctttggggta tctcttgttt tttggggaaa
    17581 gcaagaaata agctaatata tgctattaaa agagacacat ttcaaagcca gtatagcata
    17641 gaaactttta atgagatagt tttttttaca attatgagaa tttattttgt tatttaatga
    17701 ttttgattcg ttttcatcaa gtaatgaaaa tgataaaata aacatacttt aggattaaag
    17761 gagatttcta atgaaaaaac ttaaattatt cttaccaata ataggattag ctattctttt
    17821 atcaggctgt tcaaagtgga ttgatcgagg agaatccatt acggctgttg gctcttctgc
    17881 tttacagcct ttagtagaaa cagttgctga aacttaccag acagaaaatc ctggaaaatt
    17941 catcaatgta caagggggcg gaagcggaac tggtttgtct caagtacagt caggagcagt
    18001 cgacatcgga aactctgatt tgtttgcgga agaaaaatcg gggatcgatg cctcagcatt
    18061 agtcgatcat cgagtagcag ttgttgggat cactcctatt gtgaacaagg atattggcgt
    18121 aaaagacatc agtatggaaa acctgaaaaa actatttaca ggcgagatca aaaactggag
    18181 tgaagtcggt ggaaaagatc agccagtagt tatattgaac cgcgcttcag gaagcggaac
    18241 ccgtagcacg tttgaaaaat gggtattgga cggagaaaca gctgttcagg cacaagaaca
    18301 agattcaagc ggaatggtgc gccagatcgt tgccgacaca cccggtgcaa tcagctatgt
    18361 tgctttttct tatgtttccg atgatgtaga tacgttgaat gttaacggtg tagaaccgac
    18421 agatgaaaat gtgacaacga acaaatggac gatttgggca tatgagcata tgtacacaag
    18481 agatcatcca aaaaaactga caaaagaatt tttagactat atcttatctg atgaagtaca
    18541 agataacatc gttggagaac taggttatat ccctgtttct catatgaaag tcgaacgtga
    18601 ttggcaagga aatatcgtta aagagtaatc tttacacaaa atttacaaac ttagctaaac
    18661 ttttacatgt tattaaaacc aaattgatgt tcaatttgta cactgagtga gtatgttaaa
    18721 cctgattaag ggggagtcat ctgtggaaga tgtgaaaaaa gtattattaa caaaatcaaa
    18781 acgttcaaaa atggaacagc gtggaaaatt catcagtttt ttatgtatcg cgctgatcgt
    18841 gttggttgta ttatctattt tttattttgt agctagtaaa ggattggcca ccttttttac
    18901 tgataagatc aatgtatttg attttctgtt tggtacagat tggaatccaa gccaaatcgg
    18961 agcaaatggg aaaccaatgg taggggcatt gcctatgatc acaggttcgt ttattgtgac
    19021 ctttttatct gctatcgttg ccacgccatt tgccattggt gcagcagtat tcatgacgga
    19081 gatctctcct aaaaacggaa cgaagatctt acagccggtc atcgagctgc ttgtaggtat
    19141 tccttctgtc gtttatggtt ttatcggttt atcagttatc gtgccttttg tcagaagtat
    19201 ttttggcggg actgggtttg gtatcttagc tggtacattt gtgttgttcg ttatgatctt
    19261 gccgactgtc acaacgatga ctgttgatgc attaaaggca gtgccacggc actatcggga
    19321 agcttcatta gcgcttggtg ccacaagatg gcaaacaatc tacaaagtcg tgctacgagc
    19381 agccgtgccc gggattttaa cagcagtcgt ttttggtatg gctcgtgcgt ttggtgaagc
    19441 cttagctatc caaatggtca tcggtaatgc agcattgatg ccaacaggtc ttactactcc
    19501 tgcttctaca ttgacgtcca tcttaacaat gggaatcgga aatacaatca tgggaacagt
    19561 tgaaaataat gtgttatggt cattagcgct gatcttgcta ttgatgtcat tattctttaa
    19621 tatcgtgatc cgtatgattg ggaagaaagg agagctgaaa taatgaacgc aaaacgctca
    19681 gataaaattg ctacaggtgt tttatatata gtttcgggga tcatcgtatt gattttagca
    19741 gctttactac tttatatctt agttcgggga ataccgcaca tttcatggtc cttcttgacc
    19801 gaaccttcca aagcctacca agaaggcggt ggaatcggga tccagctatt taactcgatt
    19861 tatttgcttt tgatcacgat gatcatcagt tttcctattt ctttaggtgc gggtatctat
    19921 ctttcagaat atgctggaaa aaactggcta acagatgtga ttcgcacgtc gattgaaatc
    19981 ttgagctcgt tgccttctgt cgttgtcggt ttattcggtt tcttggtttt cgttattcaa
    20041 ttccagtatg gtttctcgat tatttctggt gcattggctt tgactgtatt caacttgcct
    20101 cttttgacta gaacagttga ggagtcttta caagcggtcc attacacaca acgagaagca
    20161 ggattggcct taggtttgtc acgctgggaa acagtgataa aagtggtggt tccagaagcg
    20221 acaccaggga ttttgactgg tgtgatccta agttctggaa gaatttttgg tgaagcagct
    20281 gcattgatct atacggctgg acaaagtgca cctgcgttag attttacgaa ctggaatcca
    20341 ttaagtgttt caagcccgat cagcattttc cgtcaagctg aaacattagc tgtccatatt
    20401 tggaagatca atacagaagg cacgatgcct gatggtgttt ctgtttcggc tggcgcttct
    20461 gccgtgttga ttattgctgt tttactgttc aacttcggcg ctcgtgcgat tggcaaacga
    20521 ttataccgaa aaatgacttc agcctaagga gaagaagcaa aatgaaaaaa tataactgga
    20581 atgaaacaaa tgtcatcacc atgccaaaag agaaagaagt agcactatat acggatgatc
    20641 tacatgtctg gtatggcgac aatgaagcaa tcaaaggagt ggatctggaa tttgaaaaaa
    20701 ataagatcac tgcactgatt ggtccttccg gctgcggaaa atctacgtat cttcgttcgt
    20761 tgaatcgaat gaatgatggg atcgctaata cgaaaatcac tgggaaaatc atgtatcaag
    20821 gcgtagatat taatgcgcct caagtggatg tttatgagat gcgcaaacgg attggcatgg
    20881 tttttcaacg tcctaatccg ttcagcaagt cgatttatga caatattaca tttgctttga
    20941 aacagcacgg cgaaaaaaac aaaaagaaat tagatgaaat cgttgaaaca agcttgaaac
    21001 aagctgcctt gtgggaccaa gtaaaagaca gcttggacaa aagtgccttg gctttatctg
    21061 gcgggcaaca gcaacgttta tgtattgctc gtgcgatcgc gatgaaacct gatatcttgc
    21121 tgctagacga gccggcaagt gctttggacc ctatttcgac aggaacggtt gaagaaacac
    21181 ttgtaaactt gaaagaagac tatacgatca tcatcgtgac gcataacatg caacaagcag
    21241 ctcgtatcag cgactatact gcctttttct atttaggaaa agtaatggaa tatgacaaaa
    21301 ctaacaagat atttaccaga ccaaaaattc aggcaaccga agattatgtg tccggtcatt
    21361 ttggttaaga cggaggcctc aacatgacaa aagagattat ttcatctaaa gatcttcatc
    21421 tgtattatgg aaagaaagaa gcactaaaag ggatcgatct aagcttcaat caaggggaaa
    21481 tcactgcgat gatcggtcct tctggttgcg gaaaatccac ttatctgcgt tctttgaacc
    21541 ggatgaatga cctgattccg aatgtaacga tcaccggaag tgttttatat aaagacaaag
    21601 acatctatgg tccaaaaaca gatgttgttg aactaagaaa agaaatcggc atggtattcc
    21661 agcagccaaa cccatttcct ttttctatct atgaaaatgt catctatgga ttgaaattaa
    21721 aaggggaaaa agataaaaaa gtccttgatc aagtagttga agaaagcctg aaggcagcgt
    21781 ctgtttggga tgatgtgaaa gacaaattgc ataaaagtgc tttatcttta tccggtgggc
    21841 agcagcagcg tgtatgtatc gcacgtgttt tggcagttaa tccggaaatc atcttattgg
    21901 atgaaccaac aagtgcgctt gatcctgttt ctactgggaa aatcgaaagt atgctgttag
    21961 aattaagaga gcggtatacg atgatcatcg tgacccataa tatgtctcaa gcttcacgta
    22021 tttcagataa gacggcattc tttttagatg gtcacttgat cgaatacaat gatacgaaga
    22081 agatattcat gaatccagaa aaacaagaga cagaagatta tatttcaggt cgatttggat
    22141 aaggaggaaa cggaatgcta cgtacacaat ttgaagaaga attgctgaat ctccataatc
    22201 agttctacga aatggggatg atggtgagca gcgccgtgca caagtcagtt cgtgcataca
    22261 tcaaacacga taaaatctta gcccaagaag ttatcgaaaa cgatataaat atcaataaca
    22321 tggaaacacg cttggagaag aaaagttttg aaatgatcgc attgcaacag ccagttacaa
    22381 ctgatttgcg tatgatcatt acggtcatga aagctagctc ggatttagaa cgtatggccg
    22441 atcatgcggt gtctgttgct aaatccacga ttcgcgtaaa agggcagacg agaataccgg
    22501 aaatcgaaaa agagatctcc gatatgtccg attatgtgaa aaaaatggtg gacaacgtcc
    22561 tagttgctta tgtcaaaaca gacgaaaaag acgcacgtac gatcgccaat atggatcaaa
    22621 gagtcaatga ctatttcaat cgaatctata atgcaacaat caaaaatatg caggaaaatc
    22681 ctgagacagt catcagtgga acagactact tgaatgttgc cagttatcta gaacggatcg
    22741 gagactatgt gacaaacatc tgtgaatgga tcgtctactt ggctacaggg aaaatcactg
    22801 agttaaatac gaaccacaat gagattttct aaactgaagc tgtgattatg ccgtttgttt
    22861 aggaaaagag gttgtgaaat cagcggttgt gaaaaagctg ttctgcatca agtaataaga
    22921 acagtcaaac aaaaaatagc tcctcatatt tttcgtttgg ctggacttat taccgcagat
    22981 gcaagctttt gaacaccgtt tattcgggaa agggtttgtg acaaaacatg tcacaaaccc
    23041 ttttcatcat ttcatagaaa ggataaagaa tgcaaaaaaa aattagcatc atcactatat
    23101 tttttatttg tgtgcttact tcatgggtta ttcagcatca agccgcttta tatgaagtac
    23161 ctgtcatcga gatagaaaaa gtacgaataa aagaggatca tcaattgatt acaggaaaac
    23221 tgatgaacac ggatcaaaaa ggagaatcga tttatttatc ggttccttat caaaaaaatc
    23281 aaatagaaac agcaaatata aaagcgagac aaatgtatct agtccaaatg catggagatg
    23341 aatggcagct agtagcaaaa aaaaatgatg gctggctttt cttcttcaca agtatcttca
    23401 ttggcgccat gttgcttatt ggtggaaaac agggtctatt tgctttagga agtctcatca
    23461 tcaatatcgt tttactttta cttttgttgc tcatctttac taaaacagaa gggatttctc
    23521 tattaaatat atctatcctg ttcgtcgtta tagggatgat tattagcata ttggctttag
    23581 atggatggaa tagaagcagt ataatcaaaa tttcagctgc agttacgagt gtttttttag
    23641 cattttttat ttgtcttttg acaatgaatc attggaaaga ccaaggactg cgctatgagg
    23701 aactgaatta tctgacacga ccgtatcgtt ctgtttttct ttctagcgta ctgattggaa
    23761 cagctggagg aacactcgat accgttatca cagtcattgc gacattagaa gaattgacta
    23821 agcgacaacc agaaataaca gcgaaacagt tatggctttc aggaaggcaa gtgggtcgag
    23881 atgtgattgg ctcgatgacc aatatccttt tgttcgtcta tatcagtggg tcagttccat
    23941 cgcttctttt atatttagga aatcaatggt cgttcaaaga aacgatagaa cagcatttat
    24001 cattggaatt tttacgcgtc attgctggca gcttaggaat agtattatcg atccctatct
    24061 cggtcctctt ttttctgact gtaaggaggc tgaaacgatg aatgtactta ttatacttgt
    24121 tgggatttta gcaatcagcg tgctacttat tggcggtacg caaggtatgt atattctttt
    24181 aggcttattc atcaatttag ggatcttttt cctgttgtta ttcggttatc atcagaagtg
    24241 gccgatactg gcattgtcgg tcataggatt tttattgatt gctgtggtta tcttattttt
    24301 tatcaacggt tataatctaa aaatgcgtgc agcttttgtg agtatactga tctttttatt
    24361 ttgtttccta ctattgcttc ctatcactga ttttttagca atccaaggtt ttacatcggt
    24421 agaactagaa gaattatctg gcttagataa gactttagcg attgatttta gattacttac
    24481 gagatcgttg cttctgatta gtcttagtgg tgctgtttta gatgcgagtg tagcaatttc
    24541 tagtgggaca ttcgaagtat atcaggcaaa tccgcaatta tctttttacc aattgcgcca
    24601 ttctagtttc gctattgcta aaaaaatatt ggcgtcaaca gtaatgacac ttctttttgc
    24661 attcatggga agttctttag cgttgatcct ctggtttatt gatttagaca ttccttttca
    24721 gcaagtgatc aatgaaaaat catttgttct agaatatacg atggctattc ttaccacatt
    24781 gtccgcacta cttgttcttc ctttaacctg cgtgactaca gcatatctct ttacaaaaaa
    24841 gaaagaacaa ctcaagatgg agtaaaagtt acaacttaag tcctatgaca tttttactga
    24901 atcagtgcat aatatggatg taaggaaaag cttgcaaaca attttgctta aggaggcaat
    24961 ttccatgaaa gaaagagaac gtattttaga tttagtgaaa aaaggaatcc tttcgactga
    25021 agaaggctta gatttattag aaagcatggc aactgaaaaa gacgaaaagc aaatcaaaaa
    25081 agaagcagac aaggtaactg ctagtcataa agaaaaagat caggcaagtc aattgatcga
    25141 taaacttgaa aacggcgaag aagaaatttc tgagccagtt gatccaaagg aaagagaaag
    25201 acaagatcaa gaaaacttag aaaagatctt agatgaacta gcaactgaag cgaataaaac
    25261 ttcggcgcgt ctagatgaag taaatgctca attcgctgac aataaggcag cacgcaatga
    25321 gaaacaggaa gcattgatgc agctgaatac aaaagaagaa ttaggcgagt tgacagaaga
    25381 agaattagca cagcgtcaga cgttagaagc agaaatcaaa gaattagaag ctagtggcga
    25441 tacactgtta gaagaacaat tgaaattaga agcagaatta aaagatatcc gtaagaatca
    25501 atggtctgag aaaaaagaaa cgtttactga taagtttgaa ttgccagatg attggaaaga
    25561 tcaagcaaca gatacactga atcaagtagg cgaaaaaatg tcagaagctg gatcacagtt
    25621 aggcaaattc ttgaagaaaa cattccaaac tgtttcagaa acagtgaatg acaacatgga
    25681 atggaaagat gtcagcttac gtgtccctgg gattgcgaca acgaaatttg agcatgaatt
    25741 ttactatgaa gctcctgcag caagtatcct tgatatcaag gcagcaaacg gaaacgtgac
    25801 gttaaaaacg tgggacagca acgatgtgaa agttgaagct aagatcaaac tttacggaaa
    25861 aatgggtgct gaaccatttg aagcattctc tgaacggagc cagattgagg ttaatgaaga
    25921 tcatatctcc ttccaaattc caaataaacg tgtgcgtgca gaccttgttt tctacttacc
    25981 taaacgagta tacgaccacg cagctattaa acttctaaat ggcaacatca tgattgaaac
    26041 attggaagct aaagatattt atacaaaatc tacgaatggt aacatcatcg tggaccaatt
    26101 aactgcaacg atgctggaag tagaaggtgt aaacggcaat atcgatattc gaaacgggaa
    26161 catcttagat tctattatcg aaacagtaaa tggtacagtg acatttggtg cgacagcaga
    26221 aaacctttct gtgtctttgg taaatggtga tgtacgtctt acgattaaag aagataattt
    26281 gaaaaaagta gaagcaagtt cagtgaacgg aaatgtcaaa gtcgctttac cagatggcat
    26341 cggtttggaa ggacatgcta aaacaagtct tggaagtatc aacagccgtc tgtccaatta
    26401 cgaagtagtt cgtgagaaaa aagaacggac aaaccaaatg ttgcaattcc gtcgtgtttc
    26461 agatggtgaa atcgcgcaag tgcaactttc aacgacaaca ggaagtatct atttaaaaga
    26521 tacagataaa taaagaggtt gtgaaagaga tgttcagcct tgagcaataa gaagcgattt
    26581 tgtaaaatag cttcccctat tttacaaaat tgtgccttat tgccgatagt ctatctcttg
    26641 aacaccgttt accaagagat tatgaacctg ccaattatcg ccgagtcaca aggaataatt
    26701 tcagaaaaag ctcgtcacgt tttatgaata tggtaatttg tgaccgagaa ttttgcagaa
    26761 agacaccgat taagaagaag gttgtcacaa cgattttctt catttaaaat agtagaacag
    26821 aggtgagaca tgatgaaaaa aaagttgaca aaatctaata aaaatgttgt tttgacaggg
    26881 actttagcag gaataggaga atacatcggg attgatccga ctgttatccg tgtgctttat
    26941 gtattcctga gcttagttgc ttttggaagt ccaattattt tatacatttt attagctttg
    27001 attatcccat ctgcgcgttc tgcacgaaca aactatggac ataacaatgc atattatgaa
    27061 aaaaacaatt atcgtgaaca aactcgtgaa caaacaaaaa aacgcaaaga agcggaaaaa
    27121 atcgatgatg acgaatggag tgacttttaa tgtcgtactt tcaacgtatt ttagttaata
    27181 cgttaacatt catatcattg gctgttcttt ttcctgcgat ggtccatgtg agcagtattt
    27241 ggacggccgt tcttgccagc ttcgtattgt ctgtgttgaa tatgtttgtc aaaccgattt
    27301 tgacgatact gtccctgccc tttacattag tcacattggg attgtttacg tttgttatca
    27361 atgcagcgat tcttaaaatg acctcctttt ttgtgggagc agaacatttt agtttttctt
    27421 cattcggtgc agcgttgtta gtagctatct tgatgtcgct tgtcaatgta attgtcagcg
    27481 aacataacag tagaaaataa aagaaagctg tgccgaacta tcggcgcagt ttttttattt
    27541 agaatcagga atgcgttttc tattacattg gtaattaaat aaaaacgttt tttttaattg
    27601 gtagtacttt tggctagaca atgataaaat gatagagaac actctaatag aaatgaggta
    27661 gcatgaatgt cagaagttgt aaaagttagc caattagtga aaaaattatc tttggaaatc
    27721 gtgacaggcg acgagaaaag cttagaccgt gtcatcgtaa ctggggacat ctcacgccca
    27781 ggactagagt tgactggtta ttttaattat tattcccata atcgtttaca gctatttggc
    27841 agtaaagaaa tcagttttgc ggaacggatg atcccagaag agcgtttgat catcatgaga
    27901 agattatgta gtgaagatat gcccgcattt attatttcaa gaggattacc agctccagaa
    27961 gaaatgatcc aggcagcaaa tgaaaatggg atagctgtac tgcgttctcc catctcaact
    28021 tctcgtttat taggagaatt atccagttat ttagatggtc gccttgcccc tcgaacaagt
    28081 gtccacggtg ttttagtgga tgtttacgga ttaggtgtac tgattcaagg tgacagtggg
    28141 ataggaaaaa gtgaaacagc gttggagttg atcaaacgcg gccatcgatt gatcgcagac
    28201 gatcgagtag atgtttatca gcaagatgaa ttaacggtga tcggagaacc gcctaaaatc
    28261 ctggaacatc tgattgaaat ccgaggaatc gggatcattg atgtaatgaa tctttttggt
    28321 gcgagtgcgg tacgtggctc tatgcaagtc cagttggctg tttatctgga agcatgggca
    28381 aaagataaga aatacgaccg tctgggctct gaagacacga ccgttgaaat cgcggaagtg
    28441 ggcattccac aaataaaaat ccctgtcaaa acaggacgaa acgttgcaat catcattgaa
    28501 gtagccgcga tgaatttccg tgcacgtaca atgggctttg acgcaacgaa aacatttgaa
    28561 gaaagattaa gtcgcttgat tgaagaaaac tcaggcaacg actagcaagg agtgacaata
    28621 tgttagcaca aatcaatcga gttgcttttg agcttgtagg gattcctatc tactggtatg
    28681 cattgatcat cgtatcaggt atcattatcg ctatgtggct tagcagcaga gaggctgttc
    28741 gggtaggatt aaaagcagat gatgtgacag attttatgtt gtggggattg cctatctcca
    28801 ttattggcgc tcgcttatat tatgttttat ttgatcttcc ccagtatata gctgatccaa
    28861 tccaaatatt caatatccgt tcaggaggct tagctattta tggcggactg attgcaggcg
    28921 cgatcacgct gtattttttt actaaatatc attttatttc tttgtggact tttttagata
    28981 tcgctgcacc aagtgtatta ttagcacagg caatcggtcg ttggggaaat ttcatgaacc
    29041 acgaagcata tggaccagac acgacgcgaa gcttcttaga aagtctgcat ttaccgcaat
    29101 tcatcattga taatatgtat atcgaaggga tctatcgtca accaacattt ctttatgaat
    29161 cactttggag tttgattgga tttattttgc ttttgttttt acgaaagaaa ccacaccttt
    29221 tgaaaaaagg cgaagtcctt ttatgttatc taatgtggta tagtttcggg cgtttcttta
    29281 ttgaaggatt acgtacagac agtctttact ttttaggtgt gatccgtatt tctcaattat
    29341 tatctgctgt gttatttgtc ggtacattga ttctatttat ttggcgcaga aaaaaaggaa
    29401 atttaccgga ttatgaccgc tcattaggaa aaaatcaaca aattatctga tttttagagc
    29461 gttaaaaatg aaaaaaattt caggagtggt aaaatgaatt atagagtagc ggttttagga
    29521 ccaggctctt ggggtactgc tttggcacaa gtcctcgccg aaaacggaca tgacgttcgg
    29581 atttggggac atcgtgcttc acaagtagaa gaaatcaaca cccaacacac gaatcaacgt
    29641 tatttaccag atcattatct acccgattcc atccaagcat tcaccaatat ggaagatgca
    29701 gtaaaagacg cagatgcggt gctattcgtc attccaacga aagcaatacg ttctgttgca
    29761 aaagaactag tgcctaaatt gaatacaaag ccagtgatca tccatgcaag taaaggatta
    29821 gaacagggaa cacataaaag gatctctgac atcttgttag aggaaatccc agcagaaaaa
    29881 agaaaagctg ttgttgttct ttctggacca agccatgcag aagaagtagc aaaacatgac
    29941 atcactacga tcacagctgc aagtacagat gcaaaagcag caaaatatgt acaagagctg
    30001 ttcatgaata attattttcg gatctatacc aatcctgatg tgatcggtgt agagatgggg
    30061 gcagctttga aaaatattat tgctattggt gctggcgcta ttcacggctt agggtttggc
    30121 gataatgcaa aggcagcaat catgacgcgc ggattagctg agatcagccg tttaggtgta
    30181 gcaatgggtg ccaacccgct gacctttatc ggattaagcg gaataggcga tctagtcgtt
    30241 acttgcacaa gtgttcattc taggaactgg cgcgcaggca acttgttagg acaagggcag
    30301 cccctagaag aggtgctagc caatatggga atggtcgttg aaggtgtgaa tactacccaa
    30361 gcagcgatgg aactagccca aatgatggaa atcgaaatgc ccatcactca aacaatctat
    30421 gaagtgctgt atgaacaaaa agaaatcaaa acagccgcaa aagaaatcat gttacgtgat
    30481 ggaaaaaaag aaaatgaatt ctcattaaca attttttgaa ggagcgaatg tatatgaaag
    30541 ttaaaaaagc agttattcca gcagcaggat taggaacaag atttttacca gcaacaaaag
    30601 ctatggcaaa ggaaatgttg ccaattgttg ataaaccaac aatccagttc atcgttgaag
    30661 aagcgttgaa atcaggtatc gaagacatct tgatcgttac aggaaaagct aaacgaccaa
    30721 tcgaagatca ctttgattca aatattgaat tagaaacaaa cctaagtgaa aaaggaaaga
    30781 ccgaattatt gaaattagta gaagaaacga cagatgtcaa cttgcatttc atccgtcaat
    30841 ctcatccaaa gggtttagga catgccgttc ttcaagcaag agcctttatt ggaaatgaac
    30901 catttgtcgt tatgctaggc gatgatataa tggaagacga agtaccattg acacagcaat
    30961 taatcgatga ttacgaacgt actcatgctt ctacgatcgc cgttatgcaa gttcctcatg
    31021 atgaaacttc aaaatacgga attatcaatc caggagaagt attggaagat gggttataca
    31081 atgtcaaaaa ctttgttgaa aaaccagatc caagcaaagc accaagtgat ttagctatta
    31141 ttggacgtta cttattgact ccagaaattt ttgatgtatt gtccgagcaa caaccaggag
    31201 ccggaaatga aatccaacta actgacgcaa tcgatacatt gaataaaaca caacgcgtat
    31261 ttgcccgtaa attcacaggt aaacgttacg atgtcggtga caaatttggt tttatgaaaa
    31321 caagtattga atatggtcta acacatccag aagtaggtgc acctttacgt gagtacatcc
    31381 tgtctttagg taaaacatta gaagcagaag gaagcaagat cgatcataat ccaaaaggaa
    31441 taacagaaat caaagaagaa aagaaaaaat aacccttttt tctaacgagc ttcagctgct
    31501 aattgaataa aaagtagttg aagctcgttt ttatttgttt aaaaaacacg aaaatgctat
    31561 ccttatagaa aagagagaca agaagggagt ttgatcatgt caggaggaga aattgcagga
    31621 ttgatcgcag caatcgcctt tgcgatactt gtcgttttta tcgttcgtgt gttgttgcaa
    31681 gtttctaaaa ctttagaaaa agtagatcag actgtagcag aagcaaatac gacgatcgaa
    31741 atcgtcacaa aagacgtaga tcttttgtct agacaagtag aaggcttact ggtcaaaagt
    31801 aatgaactgc tcttggatat caatggaaaa gtcgcaacga tcgatccatt gttcacagca
    31861 gttgctgatt taagtgaaag tgtatcagaa cttaatcatt ctagtaaaaa catcgcgacg
    31921 aaagtcggcg gaatcggtaa aacgacagct aaagcgacgg ttgccggaaa agtcggtggg
    31981 acagcgatga aattttttaa aaacaaaaaa catactgaaa cgacaggagg aaaataaaaa
    32041 tggctaaaaa aggtggattt ttattaggtg cagtagtcgg aggcacagcc gcagcaatcg
    32101 ccgcgttatt gttagcacca aaatctggta aagaacttcg ggatgaatta gctgcccaag
    32161 cggatgattt gatggatatg gcttcagatt acacagatat ggcgaaagaa aaatcgactg
    32221 agttaacagg tattgcaaaa gaaaaagcgc aagatctttc tcaacaagca ggtgatctag
    32281 caggtagtgt caaacaaaaa gtaagtgaat cagcagatga agcaggaagt gaatctgacg
    32341 atatcttgtt aaatcttaaa gaacaatcag ctgacttatc taatcgtttc aaaaaaacgg
    32401 cagaagatat gaaaaaacct gcagatgaat taggaagtat tgctaaagat acttcagaag
    32461 atatcatgat tgatgtaaaa gacgctgctg gtaaagcagg agatactgtc aatgaaggaa
    32521 tgtcagaagc aaaaccagtt gcagatgact caaaagaaat gatgaaaatg gcaaaagaag
    32581 atgcgaaaga agctaaaaaa gaaataaaag atacaatgac agattcatct acaaatcact
    32641 aaaatcacaa atttatagaa taaaaaggtg ggagcggact agctctcacc ttttttactc
    32701 ttatttttct aaaacaagta attcttttgg tgtagtcgtg aatggcacac agccatcgct
    32761 tgtcacatat acacagtctt cgatacgtac ccctactttt tcaggaatat agattcctgg
    32821 ttcaagagag aagcacatcc cttcttcgat tactaagtca tttccttcta caagggaagg
    32881 gaattcgtgg acggttgttc cgatcccgtg acctaaacgg tgattgaaat attcgccgta
    32941 gccatagctg ttgatcacgt ttcgtgcgat ttgatcaagt tcacctgctg tcacacctgg
    33001 acggacggct tcttgtgcgg caagctgtgc ttcaagagtg atgttgtaga ttttttcttg
    33061 gaagtcggtt ggtttttgat aagcaattgt acgagtagca tcactgcaat agccattcca
    33121 aacaacgcca agatcgaata acacaagttc attcggtgaa atcttcgtat ttcctggtgt
    33181 accatgaggg cttgcagcat ttgctcctgc aaggactaat gtgtcgaaag acatttgtga
    33241 aacaccttgt tttttcaatt ggtattcaat ttctgcaatg atcgcttgtt cagtcacgcc
    33301 ttctttcact gctttaaagc caatctcaaa tgcaacatcg gcccatttac cagcttcgat
    33361 cagtttgtct ttttcctcta gagttttgat cagttgtaga cgttggatga tggaagttag
    33421 atcagtgtaa gaatccgttc ccgtcaagat atcagagata gcatcgaagc gctcgacagt
    33481 caaggcgttc ttttcaatcc ctacattgcc aggctgacca tagcgagcac gaagttcttt
    33541 tccgatgatc tcccaagggt tttcgctgtc taaatagcca cggacatcat acgcccaact
    33601 actgttttga gcatcttcaa cttccaaagc aggagtgaaa aggaaaggat cttgttcaag
    33661 agggataaaa agagctagta cacgctcatg aggatcactt tcgtatccgg aaaagtaagc
    33721 gatgtggcca gggtcgctga tataagccaa ttcgacttgg tgttgtgcca tccattgacg
    33781 tagttcattg atttttacgt tattcatttg gcaacctact ttctaaatta attttgtatt
    33841 caataattgt accactgatt tattaaaaga ccaacgttct aaaaactgaa aaaagtaaaa
    33901 attttcatga aaacagagat aaaacggttg caattcttga gattatttga tattctattt
    33961 tagaaatgta agacgtattt tcagaaaaca tataaaatat gaataggaga acacaaatgg
    34021 aaaagcaaac aatcactatc tatgatgtag cgagagaggc aaatgtttcg atggctactg
    34081 tttcacgtgt cgtaaatggc aatccaaacg ttaaaccagc aacaagaaaa aaagtcttgg
    34141 aagttatcga tcgcttggat tatcgtccaa acgcagttgc gcgtggcttg gcaagtaaga
    34201 aaacaacaac agttggtgta atcatccctg atgtcagcaa tatgttcttt gcttctttag
    34261 cacgtggtat cgatgatgtg gcaacaatgt acaaatacaa tattatctta gcaaattcag
    34321 atggcaacga ccaaaaagaa gtcaatgtat tgaataactt actggctaag caagtggacg
    34381 gcgtgatctt catgggtcac catatcacag acgaaatccg tggagaattt tcacgttcaa
    34441 aaactcctgt tgtattagct ggctctattg atccagatga acaagtaggt agcgtaaaca
    34501 ttgattatgt atccgcaaca aaagatgcag taaacaaatt ggcaaaaagc ggcaataaaa
    34561 aaatcgcttt tgtcagtggt gcgttgatcg atcccatcaa tggacaaaat cgtctgaaag
    34621 gctataaaga agcattaagc gaaaataacc taccatatag cgaaggattg atctttgaag
    34681 cacaatacaa cttcaaagat ggtctatctc tagtcgatcg gatccataat agcggtgcaa
    34741 cagctgtcta tgtttcagat gatgaattag ccatcggtat tttggatggt ttattagacc
    34801 gtggcgtgaa agtaccggaa gaattcgaaa tcatcacaag taacaattca ctactgacag
    34861 aagtagctcg tcctcgttta acaagtatca cacaaccatt atacgattta ggggcggtat
    34921 ctatgcgcct attgacgaaa atgatgaata aagaagaagt cgaagaaaaa acgattatct
    34981 tgccttatgg aatcgaagaa aaaggatcta caaaataata aaaggacaac cattcagagg
    35041 ttgtgtgcct gccgtttaac tctgcgttac aaggagcaat ttcaaaaaac agcatctcat
    35101 gtttgatgaa tattgtgact cgtgattcgg agttggcagg agaacaccgt ttatttggga
    35161 aaaggggtta tgacaaaatt ttgttataat cctttttttg tgtcttaaaa aagagagaaa
    35221 gtggttgaaa ttacatttat cgtatataaa acaatccgtg tatagatgct gcattttgtt
    35281 caccaccatc aaaaaaataa gggactgtga caatactatt gtcacaatcc cttattactg
    35341 tgtattctat taaatcaatc atctttctca tctgattctt tagttgcttg attcgctctt
    35401 gcataagctg aagcggtgtt ccagtaatct gtatagtttt catcggtacc gccaacgcca
    35461 aatttaaagg cgcttttctt cggtccattt ttcgcccata gcgattcaac tgtttttcct
    35521 ggtgtttgga tggatttttt attgacagta acttttccag gtgtttgacc agtaaatcca
    35581 gatacttttt tcttcatcac atcagatgat aattcgaatt tctcatcagt gccaaaaatg
    35641 tcaggttttg cttggtaagc ttgattgatc agattggcaa gataattagc cgaacgtatc
    35701 cttgctttgc tgtccattcc agtattgtca tcgtgccctg cccagctact gatggtaata
    35761 gacggagtag agacaattag ccatgaatct cggtattcat tggtagaacc agtttttccg
    35821 acccaatctg cttctcctag atttccgttt aagcttgaga tggcatcttt gaatggtgta
    35881 gtgatttttg cattgatcac actacgcatc atgtcattca tgatcgatgc cgttgcttcc
    35941 gaatagatcc ggacagagtt gctttcgtgt ttataaatta catttcctgc gttatctgtg
    36001 atggaatcga tgatatagcc ttcttcatag acgccgccgt ttgctaaagc ctgaaatccg
    36061 ttggtctgtt gaagcgtcgt gacatctact gtacctaaag gagcagattc aacgccccaa
    36121 ttgtcatttg ccgggtagtt catctttgcc aagtagtgct cgtaagcata ttgtttcgat
    36181 ccaccggaat tcaacacatc ttgatataag tgataagccg ggatattgtt ggaccattct
    36241 agtgactcac ggacggtttg gaaggtgttc gatccttcgt tagtagcatt gacgattttc
    36301 tcgccagcat tttctccttc ttgccaagtt gttgcataat ctgatacacg ggattctgat
    36361 ccgataagcc ccatgtcgat tgcaggtcca taaacaagaa ctggtttgat tgatgagcca
    36421 gcttgacgtt ctgtatcgaa ggcatggtta ttttgattct gactgtaatt ccgtccgcca
    36481 acgaatccgt agatgcggcc agttctgtta tccatcaata catttccggt ttctacttga
    36541 ctgccagcac catcatctaa catgtatcca taattttcaa cgccattttg caatgcagca
    36601 tagacatctt tatcgattgt cgaatggatt gtatatcctt tattttggat cgtttgctgt
    36661 gccttttgat aataagcgtc actgaccgat gaatcagaca tatcagcgtt gtctttttca
    36721 gccaattgtt gtgcaatgat tcttgttgct tcattcatca cagtataata caagaattct
    36781 ctgtcagtct gttctgcgat ttgttgtggt agaaagtctt ttgtaaggtc ataggctttt
    36841 gcttcttcgt attgttcttt cgtaatttgg ccttcacgat acatactgaa taaaacaaaa
    36901 tcttttctgg caaggccggc agaaagatct tcttttagcg cgcctgtgtt cgtgtatgga
    36961 ctatacgtga tcgggctttg gggtaatcca gcgatataag cagcttgcgg cagcgtcaca
    37021 tcttttgcat ctacaccaaa gattcctttt gcagcttcct ggactccagc aatgttttgt
    37081 cccttattat tacgaccaaa tggtgagaca ttcaaatacg tagcaatgat ctcatctttt
    37141 gaaaagtatt tttctacttg tgcagaaaga atgatctcgt ttgctttacg tttgaatgtt
    37201 gtttcatctg taaggatctg ctgtttgact aattgctgtg tcaaagttga tccaccagaa
    37261 gagccaatac cagtagcttc agatactaat gctcgtaaaa cagctttagg aacgtaacct
    37321 tggtgtttat cgaagtattc atcctctgtg ctgataattg cagttttcaa taaaggtgaa
    37381 atcttatcag atgaaatagt cgtgcgcatc agatctgtct ggatttttga tatctctgta
    37441 ttatctgcat aaacaatctt tgacgtttct gtaatgtttc ctagttctgt ctgcagtgct
    37501 tttttagtcg gcaattgtgt gtcttcgacg agataggcaa aatagccagc gcctattcca
    37561 atacctagag ctgcgaacat caaaagaaga acagtaataa aaacagtcaa tgattgaaaa
    37621 actctaaaaa taatcttagg aacaaaccat ttttcttttt ttcttctatt ctgttttttt
    37681 cgagtatttt tgcgtggcaa aagggtcacc tcaactaatg gttttatttc gctaattata
    37741 gcaaataata gaacaaaaaa acagacaagg ctgtttttgg caggaatttc ttttttaaaa
    37801 gagttaaaac gtgagaaaat gatctttatt ataaaagagt tcttaagctt ttctttagag
    37861 aaaatttcag ttactatgga gaacagggca aagaaaaatc aatcattttc tttgtatatc
    37921 atcttaccaa tatataggaa ttgttagtaa caattgcctt tcaaggagga atccatccat
    37981 gttattaggc tcacacgtca gcatgagcgg aaagaaaatg ctgttaggag cagcagaaga
    38041 agcagccagt tatggatctt cgacttttat gatctataca ggcgcccctc aaaacactag
    38101 aagaaaagcc atcgaagaga tgaatattga tgccggaaaa gcgtacatgg cagaaaaagg
    38161 tctaacaaat atcgtcgttc atgcccctta tatcattaat ttggggaata cgatcaaacc
    38221 agaaatgttt ccttttgccg tccaatttct gagagaagag attgttcgtg cggaagcttt
    38281 aggcgctact caaattacac ttcacccagg tgctcatgta ggggcaggaa cagaagccgg
    38341 aattgctaga attattgaag gcttgaatga agttttgaca aaagaacaga cggcacaaat
    38401 cgctttggag acaatggcag gtaaaggtac ggaaatcggt cgaacatttg aggaattagc
    38461 gcaaatcatc gatggagtaa aattgaatga aaaattatcc gttacttttg acacctgtca
    38521 tacgaatgat gccggttata atattaaaga agacttttca ggtgtaatgg aagagtttga
    38581 taagatcatc gggttagatc ggcttaaagt actccatatc aatgattcta aaaatccgca
    38641 aggttcgcat aaagaccggc acgccaatat tggttttgga acaatcggat tcgacacgct
    38701 gaataagatc gtccatgatc caatgtttga caatatttcg aaaatcttgg aaactcccta
    38761 tgtaggcaaa gataaaaaga aagcttacgc accatatgga aaagaaatcg ccatgttcaa
    38821 agcgcaaaca ttcgatccag aagtgtttcc tgagttatta gcagaagaag tgatactcta
    38881 agattttcta tctgacagca gcaaaaaagg accaggcaca ttgccttgtc cttttttgtt
    38941 taagaaacaa aggagtactt tttgcagtct ttttcatcat gcatcttctc ccccctcttt
    39001 ttaaatttag acaaacaagg tatactggta gaaaagtaaa aaaacgaaaa gtttcacaag
    39061 caagtggcaa aacgtgattg gaaggacgaa aagatgctga acactttaaa acccttgatc
    39121 aaccaactaa aaaagaaaca tacatacttt attcttggca tagccctatt atttacaatt
    39181 attcgtttgt atttatatag taaagcaatc tatggaacag acatgtcggc aacctatgat
    39241 gaccagctgc tgattcgcta tgctcaaaat attttggacg gtaaatggct aggggagtat
    39301 acgactacaa cactttccaa aggaatctcc tacagcttat ttttagtact tgcacataaa
    39361 ctgcatcttt cttatgcagt tttgttcggc atgttcagta tttttgccag tatattggtt
    39421 gcactcgcgt ttaaaccgat tgtgaagaac aagtatcttc ttttaagtat ctatttttat
    39481 tttttgtttt ctccgattag ttttacacat gaatattcca ctcgaacata tcgtaatgcc
    39541 ctcgtgattc cggctgttga gatcattatc gcttgtttat tagctattta ttaccgcaaa
    39601 tacggttcta gcagaaaatt gctgccatgg tttttagggc tttctgtcgc ttttcctttc
    39661 ttttggttca ttcgcgaaga ttctatctgg cttcttcctt ttactgtagt agctttgggt
    39721 atcagtttcg tccagatctt gctagggcaa gacttttctt tcaaagaagt aaagaagtta
    39781 aaaaaacgac tctttcttct ctcgaaacgg gaatggctga aactttcatt gtttttactt
    39841 cctatagttg gcttttccat cgtctatcaa tggatcgaat ataaaaatga gacaacatac
    39901 ggtattgcaa ccatcaatga ccgttccagt ggagaatttg gacgtttgat gaaacaactg
    39961 atccgaatag atgatgaaac gaacttgaat aaagaaaaca gtgagatatg ggtctcacat
    40021 gctgcactaa ataaagcatt agccgcatct cctacattcc atactgtttc tacccgtatt
    40081 gacgaattat atacgactca cgcgtggact caaggtggaa aagtaaaaga attacccgga
    40141 gatatcatct tttgggcatt gcgagatgtg gtaagcgaat ctggttatta ccaaaacaat
    40201 gcaaaagaaa ccaatgattt ttggaaaaaa gtcaaccaag aattagaaga agcctatcac
    40261 aagggaacaa tcaaaaagaa aaaagagttc tatctgatgg gatcaggaga tggaaagacg
    40321 atgagtgatc tgccaatcgt attcgatttc cttcaatcag gatacgctta tgcatggcga
    40381 tataaagatt tccatcaagg aggaaattat agtttcggaa cgcaagaaca agtcaaacag
    40441 gctcaagaat tattaaacct tccgctaatg gagaattgga tcgatagtaa tgaaccgaga
    40501 acacctaatt tccaattaac aaaatcagca aaagtcgcga atgtcatcat aaaaatttac
    40561 caagttctgg ctattcccgt gctgattgct tcaatagcag ggatattact tatgatacta
    40621 ggaaccgtat ttgcaaaagc tgatcgtgcc tattatagtt cctctttgat tgtggtcacc
    40681 ggattaatac tgacacaatt ggtttttctt ttcggtgtct catggttttg ttcttatgcg
    40741 ccagaaaaaa aggactattt tttaagtgtc tacactggtg caggcgtgcc attaacgcaa
    40801 ttcatcgtag taacaagcat gctagttttc agtaaattaa atctgaaaaa agctttatct
    40861 tctaaataaa aagacccttg gtagctagat ttt
//
LOCUS       DAZABD010000018        36980 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000018 DAZABD010000000
VERSION     DAZABD010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36980
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.018"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            76..3354
                     /locus_tag="ACHALG_001037"
     CDS             76..3354
                     /locus_tag="ACHALG_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HFN2586461.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3895..5802)
                     /locus_tag="ACHALG_001038"
     CDS             complement(3895..5802)
                     /locus_tag="ACHALG_001038"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HFN2586462.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            5932..6807
                     /locus_tag="ACHALG_001039"
     CDS             5932..6807
                     /locus_tag="ACHALG_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HFN2586463.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            6887..7861
                     /locus_tag="ACHALG_001040"
     CDS             6887..7861
                     /locus_tag="ACHALG_001040"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFN2586464.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            8041..9186
                     /locus_tag="ACHALG_001041"
     CDS             8041..9186
                     /locus_tag="ACHALG_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFN2586465.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            9323..9667
                     /locus_tag="ACHALG_001042"
     CDS             9323..9667
                     /locus_tag="ACHALG_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HFN2586466.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            9938..11224
                     /gene="efmA"
                     /locus_tag="ACHALG_001043"
     CDS             9938..11224
                     /gene="efmA"
                     /locus_tag="ACHALG_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HFN2586467.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(11362..11961)
                     /locus_tag="ACHALG_001044"
     CDS             complement(11362..11961)
                     /locus_tag="ACHALG_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFN2586468.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            12365..13486
                     /gene="mnmA"
                     /locus_tag="ACHALG_001045"
     CDS             12365..13486
                     /gene="mnmA"
                     /locus_tag="ACHALG_001045"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFN2586469.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLIYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            13732..15153
                     /gene="arcD"
                     /locus_tag="ACHALG_001046"
     CDS             13732..15153
                     /gene="arcD"
                     /locus_tag="ACHALG_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043858 - arginine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HFN2586470.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            15167..16285
                     /locus_tag="ACHALG_001047"
     CDS             15167..16285
                     /locus_tag="ACHALG_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HFN2586471.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(16345..17637)
                     /locus_tag="ACHALG_001048"
     CDS             complement(16345..17637)
                     /locus_tag="ACHALG_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFN2586472.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            17787..18347
                     /locus_tag="ACHALG_001049"
     CDS             17787..18347
                     /locus_tag="ACHALG_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN2586473.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            18476..18853
                     /locus_tag="ACHALG_001050"
     CDS             18476..18853
                     /locus_tag="ACHALG_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HFN2586474.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            18786..20858
                     /locus_tag="ACHALG_001051"
     CDS             18786..20858
                     /locus_tag="ACHALG_001051"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFN2586475.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(21069..22016)
                     /locus_tag="ACHALG_001052"
     CDS             complement(21069..22016)
                     /locus_tag="ACHALG_001052"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFN2586476.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            22262..22726
                     /locus_tag="ACHALG_001053"
     CDS             22262..22726
                     /locus_tag="ACHALG_001053"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFN2586477.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(22788..23831)
                     /locus_tag="ACHALG_001054"
     CDS             complement(22788..23831)
                     /locus_tag="ACHALG_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HFN2586478.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(24208..25173)
                     /locus_tag="ACHALG_001055"
     CDS             complement(24208..25173)
                     /locus_tag="ACHALG_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HFN2586479.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            25407..26252
                     /locus_tag="ACHALG_001056"
     CDS             25407..26252
                     /locus_tag="ACHALG_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFN2586480.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            26443..27630
                     /locus_tag="ACHALG_001057"
     CDS             26443..27630
                     /locus_tag="ACHALG_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFN2586481.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            27636..27992
                     /locus_tag="ACHALG_001058"
     CDS             27636..27992
                     /locus_tag="ACHALG_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HFN2586482.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            27994..28332
                     /locus_tag="ACHALG_001059"
     CDS             27994..28332
                     /locus_tag="ACHALG_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HFN2586483.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    28408..28664
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            28727..29761
                     /locus_tag="ACHALG_001060"
     CDS             28727..29761
                     /locus_tag="ACHALG_001060"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFN2586484.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            29754..30440
                     /locus_tag="ACHALG_001061"
     CDS             29754..30440
                     /locus_tag="ACHALG_001061"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFN2586485.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            30464..31312
                     /locus_tag="ACHALG_001062"
     CDS             30464..31312
                     /locus_tag="ACHALG_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFN2586486.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            31509..32282
                     /gene="sufC"
                     /locus_tag="ACHALG_001063"
     CDS             31509..32282
                     /gene="sufC"
                     /locus_tag="ACHALG_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFN2586487.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            32295..33581
                     /gene="sufD"
                     /locus_tag="ACHALG_001064"
     CDS             32295..33581
                     /gene="sufD"
                     /locus_tag="ACHALG_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFN2586488.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            33578..34813
                     /locus_tag="ACHALG_001065"
     CDS             33578..34813
                     /locus_tag="ACHALG_001065"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFN2586489.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            34800..35270
                     /gene="sufU"
                     /locus_tag="ACHALG_001066"
     CDS             34800..35270
                     /gene="sufU"
                     /locus_tag="ACHALG_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HFN2586490.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            35275..36666
                     /gene="sufB"
                     /locus_tag="ACHALG_001067"
     CDS             35275..36666
                     /gene="sufB"
                     /locus_tag="ACHALG_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFN2586491.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKAVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(36761..>36980)
                     /locus_tag="ACHALG_001068"
     CDS             complement(36761..>36980)
                     /locus_tag="ACHALG_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFN2586492.1"
                     /translation="YILKKYNLPRITPHGFRHTHASLLLEAGESVKVVQQRLGHENSK
                     VTLDIYAHITNNAPKKTGQDFADMMAHQ"
BASE COUNT        12076 a         6831 c         7909 g        10164 t
ORIGIN      
        1 tttttattga cagtaaaaat aaattaggta atactttaag taaacgcttt ctaatttgtt
       61 acgggaggaa tacacttgtt aaaaaaacgt ataaaataca gtgcgctaat gatgaatctc
      121 cttatgctca gtaccccggt tttaatttct gtcgttgaag ttgctgctaa cgaacaacag
      181 tctgtgaacg aagaaaatgc acacgtgaac attgaaggga tgtcaacaat ggaggaaata
      241 ccaagaacta gtgaagatat ggatacggaa gaaggaaatg atcctatgat gaaagaatca
      301 gaaaatgaaa agacaaaaaa gcaatctgaa gaagcaaatg aagagtggaa aggggttgtt
      361 ttcggagaaa gcacaagtgc tagtaggtat gccattgaac cattagaaga cggcgtgcgg
      421 ctctcttcca cgaataacgg cggaaaattc cagtcaagcg gatcagatgg aatgacctat
      481 tattatcaag caatccccaa agacatcaac tttaggatgc gggcgacgat cgaaatagaa
      541 tcttggacct atacgaatgc ccaagaagga tttgctttaa tggtaagaga cgcagtacca
      601 aaaaatcatt tgtttgggca ggcattttat tcaaattcat ttgccatact aggcagtcgg
      661 atcgaatatg tctgggactc ggatagacaa gaagtggtca acacgagcgg cagcaaatat
      721 acgatgcgac ttggattggg gacacgggca atcacaggaa tatcatctga agatccgcaa
      781 gctgcgccag ctcctgggag tgtctcagtg gaaacaacac ctttagagac ttcagcaggg
      841 ttcttggaaa aagaaacagg ttcttataat atattcggaa acggtcatgg gaatctgttt
      901 ccggccacga actcgattac taaactggac gtagagatga aaaaaacgaa tactggttta
      961 gaggcatatt attacgatcc tgaaacagga gaagaaatgg cttctgacat catgtatgac
     1021 tgggaaaaat tatacgcttc agatgaatcg gttatttatg caggatttgc tgcagcaagg
     1081 aatatgacga tcaaggtaga aggaatcgaa gttgcttact cagatcctgc gacagatcct
     1141 cctgggcaag aaagacctac tcgcttgatt gatccgattt acactgtgac ctctagtaat
     1201 cattcaggaa accaagatca tactatgtcc tttagagcaa atgctgacgg attattgaca
     1261 attaaaaata aagcaacagg agaggtgctt aagaacgcaa aaaacttggc tatcactgcg
     1321 aatcgggaat ttgattatca gacaaaactg atggaaggaa cgaacgaatt tactttcagt
     1381 tttacgccag ataaaaacta tccgccagaa gaatacgtag agatgactag ttatgaaaca
     1441 aaagagattc ttcatacagt ggactaccgt aaacctaaat attcgacagt ttatgtaaca
     1501 ccagaagggt cagatgcagg aaacggaagc agacaaaatc cattatctct gacacaagca
     1561 ttgagatacg catatgctgg gcagacaatc gtcatggcgc ctggaactta ttccttcgaa
     1621 agaggattga cgattccaaa aggcgttaac gggacaagag aaaacccgat tcagttgatt
     1681 gcagagaaaa atccagaaaa caagcgtcct gtacttgatt ttaaaggaac tggaaatggg
     1741 atgacactgt tcagtcacta ctggggttta cgagggtttg acattacaaa tagtgcagct
     1801 atgcaaaaag gattacagat ttctggaaat cataatttga tcgaagagat tcatgcttat
     1861 cggaatggaa atacaggaat ccaaatcagc ggtagctcaa atgatccttt cgaagcatgg
     1921 cccgcgcata acttagtcaa gaactgtaca tcttttgaaa atgccgatcc aggatttgaa
     1981 gatgccgatg gatttgcggc caaactgaca ataggagaag gaaatgtatt tgacggttgt
     2041 atcgcgtatc ataatgcaga tgacggttgg gacctttttg ctaaaaatga aacaggttcg
     2101 atcggaaaag taatcatcaa aaactcagtt gcttatcgaa acggctgggt accaggaatt
     2161 gagggagaag gtaacggtaa tggattcaaa atgggcggtt cttctttaac gggccctcat
     2221 gaattgatca atagtttgtc tttcgaaaat cttgcgaaag gaatcgattc caatagcgga
     2281 acagatatta tcgtcaatag cagtacaagt tttaataacg gttcatataa tgtggctctt
     2341 tatacctctt cagcaggaaa cacagactac catgcttctg gtatcttatc tttccgaaca
     2401 gagaatcttg agatgagaga gcagatccgc ccattgaatc aaaacgaaga tcaaatctac
     2461 cactcgttga actactattg gaatgaacga gagaaacaat cagaaaatac tcttggacat
     2521 accatatcaa aagattggat cgagtctcta aatacatgtt cgaaagaagg tcagcagcct
     2581 gttacgcgct atgaaaatgg aagtatccat gttcatggac tgatgcagat taaaccgggg
     2641 aatcgtcaaa cagaaaagga aagagtggat ggtcttccat tatctgtagg tgctattttt
     2701 gacggagaaa cctcaccgtc ttcggaatat ccagagtatg caatcgtaca agaaccaaat
     2761 cagacaaaac caaatgaaca aaataatccg attattatac aagaagcaaa tggtgtggcg
     2821 gagaatgcag aaggaaatat cattgaactg aagcctttta cagtttttgt gtcaactttc
     2881 acaccaaatc aaggaacgcc tgtctttgaa gatatgcgtt atttcccaga aaagatccaa
     2941 atcgttgaaa cacagtcaag tgctcaattt tctaatcata ccgctatttt ttcagcacct
     3001 tcaaaaggag aatatacgct agaagtcact tatcgttcgg aaatatttga tggggaaaaa
     3061 tggtctgctc atacagaatc tgttacccag caaaaagcga tcactgtgcg agaaactgcc
     3121 gatgtgcccc cgggaaatga caaaccagaa agagaaaatc caggaaatga atcaccaaat
     3181 ggaaaaccaa atggaaaacc ttctcatccg gataggacag caaaagaaag tgaaaaaaat
     3241 ggcactcaag gactcccaaa aacgggagaa tttaataacc cgttgctagc attagctgga
     3301 ggaattttgt taatcggtgt agttgtttat gtcatgaaac agcgaaaaaa ataagtggaa
     3361 tttctccttt tatgatgtaa aacaattgac tgaggaaagt aaaaatagaa ggaaatgaga
     3421 ccaatagatg tctcacttcc ttctatcttt ttatagatga ggttctcctt gctgcttttg
     3481 attggctgtt cttattactt gatgcaggac agctttttca caatctctcc actaaacggt
     3541 gttcaatcag ctgaccctcg gtattagctc aacaaacggc aaaatatgag gaactatttt
     3601 tccgtttttt tctctaatac tcaggtcagg acgctgattt cacaacctct cgataaacgg
     3661 tgttcaaaag ccagtttctc ggtagtaagt caaatttatg caaaaatgtg gaaagccatt
     3721 ttcacaaaat ttttcttact acttgaaact aagcagcttt ttcacaacct ctaatacatg
     3781 gtattccaga agtaacttct tcgaaaaata agccgaaatg tcacaaaaat ttgagaagcg
     3841 attttcccaa attccttctt atttcttgaa gctgaccact tctggcacaa cctcttacca
     3901 gattcgcact cttttttctg gcgctagata catagcgtct tgttccgtga taccaaatgt
     3961 ttcatagaat tcgtcaaggt tttttggttg cacgtttgct cggagtttag caggggcatg
     4021 gacatcgatt tgcagtaaaa gttgttggta ttcttttttt gcttttgtac gccaaatcgt
     4081 tgcccaattg acaaagaatt cctcgataga aagatctgat tccgtttttg ctgcttccaa
     4141 tgcacagctt agaccgcctg catcagcaat attttccgaa acggttaatt ttccgtttac
     4201 ttttccgtca gcaaaaggca gaccatcgaa ttcttcgatc attgcttgtg ctttgttttg
     4261 aaattgtgta agatcttctt ctgtccacca gttgttcaag ttgccatatt cgtcaaataa
     4321 agcgccgtta ttatcaaatg catgggaaat ctcatgagcg ataacagctc cgattccacc
     4381 ataattagca gagcttgatt gttctagact gtaaaatggt gcttgaagga tcgctgccgg
     4441 aaagacgatc acattccgaa atggatgata ataagcattg accgtgtcag cactcatttc
     4501 ccattctgaa cgatccactg gtttgttcca tttgctgaaa cgatctttta atgtgagttt
     4561 gctgaaaaca agagtatttg aaagcaaagt accaccctga tctgcaggta ctgttttgaa
     4621 ctggtcgaat aatgctggga tcttatcggg gtagcctact tggataccta agttatttaa
     4681 ttttatgacg gctttttctt ttgttgcatc cccaagccag tcattttctt ctagacgttt
     4741 tttataaacg gcgatcattt ttttcaccat ttgttctacg tcttttttag cagcttcgcc
     4801 aaaatatttt tttccgtagt aatcgccaac tacttgatcg aattgtccag aggcaaggta
     4861 atacgctgct tttttctgag gcattgcttc gtctgttcca gacaatacgc gagaatagat
     4921 tccgctgact tggcggaatt cttctgatag ataaccacta agagaacgta ctgttttaat
     4981 gatcatccag cttttgaata acggaaaatg agcttctgtc aggatgtcat tcaaatgatc
     5041 gaagtacact ggatcagtta cgatcacttt atctggagtt gtcccaatca aacccgtgac
     5101 aagttttttc aagtccagtt tatctgtgta tttaataaat gtatcaaaag attgcggatt
     5161 atacattttg ctgtaatctg cgttttcttc tgcacttttt acatgaggag caatcaattt
     5221 atcaaattgc actgcttgtt ccgtgattgc ttctgcctga cttttttctt tgccggcaag
     5281 ctctaataac tgaacagcca tatcaaagaa aacgcctatt agttgtgcac cgttgggatg
     5341 gtctgatgca taatacgtct tatctggaag gaataaagaa ggcggataag caaagaaggc
     5401 gttcgtttgc gcattcttca tatctgcatc tacatccaga ttaaatggca atggtaatgt
     5461 atccaacagc cattctggaa attgcttatt caattcgtcg aaagactgca atgattcaac
     5521 tttttcgatt ataggttgaa gcggttgagc atgcaactca tttcttcggt cataatcgtt
     5581 tgccagttga taatatgcaa ggaaatgttt cattttttct gaagacactt tttctggatg
     5641 ggcaagcaga tcgtctgttt ctcccatcaa aagttggtcg atcccgtcaa ctaaatcttg
     5701 gaaaccgcct gtagctggtt tatctgcagg gatttttgct gttttttgcc attcttcatt
     5761 gactgcttca tagaaatctt gtttcaatcg ttctttgttc attcatcgat cgctcctttt
     5821 ttgtttcgtg tgcccattat cttacaaaat gaaggaatta acaaatagaa cataaaaaat
     5881 tgaaaattcg aacaatcaaa ctgatttgtt tttttcaatc cttcacttat aatgaaaaaa
     5941 aggaggcgat cccaactgaa tcaagtagtg gataaacctt tatatttcaa agtagataaa
     6001 aaaattaaaa aattagccag cactcagcag ctgcagtcaa gaaaaagtaa attgcttttt
     6061 ttagctctgg tttttgaaga tcaaagttat gtgattatcg atcaatcagg acatccggta
     6121 gaatattctc cagcaaaata tacttatcaa gagggtcttt cttgtaaaaa atggaagctg
     6181 atcaatgaag agccaatcga gttatctaga tggatcaaca gaaaagaaga tattcccgtt
     6241 ttgatcgaag aaaaaaggtc aggaaaggaa ctcgcaaatt gctgggtggg attgccagaa
     6301 gaacgttttt tgaggtataa gaaatgggct accccctcag gctatctttg tggtacttac
     6361 gcggcagcag ttctccttgc ctattatcaa gactatcgta aggggtggat gcttccaaat
     6421 gagattcgca agaaaaatac ggctgattcg ttggttttga cgaaggcatt gcgtagccag
     6481 atccaaccac taggacttcc tacgattcct tttcaggtta gtacagggat ttctagtttt
     6541 ttaaagaaga atggcaatca tgagcgtgca cgagcaactc ttctaggttc atggcagcga
     6601 gctacaaaac gtatacgtga aggcaaacct gtgatgattg gtattttgaa agtacttggc
     6661 agtacatatg gcaatcattg ggtgacagcg tacgcttatt ttgaaactga aacaggagaa
     6721 cgttattaca aagtacacga caactggggc gattatcata aagtcattcc tgccagttgg
     6781 agtaacggaa cagtttcact tccttaagaa tttgctgaaa ttaacagaaa atcttggaaa
     6841 aatatgttaa actatcgaag aatagagaag ttttggggag gactacatga gtaataagta
     6901 tcaggatgac acgttgaaga tttttagttt gaatggcaac cgtccattag ctgaaaaaat
     6961 cgcgaaagtg ttcggaacag aattaggtaa aagcgtcgtg aagcaattta gtgatggcga
     7021 gatttccatc aatatcgaag aaagtattcg aggggatcat gtgtatatcg ttcaatcaac
     7081 gaatgcacct gtcaatgatt actatatgga actactgatc atgatcgatg caatgaagcg
     7141 agcaagtgcg aaaacgatca atgtcgtatt gccttactat gggtatgcta ggcaagaccg
     7201 cacggcgaaa cctcatgaac cgatcactgc gaaattgatc gctaacttga tcgaagaagc
     7261 aggggcaact cgagtactga ctttagatct gcacacggtt caagtacaag gattctttga
     7321 tattcctgta gataatttat ttacgatgcc gttgtttgct cattattacc gtcaattagg
     7381 tctgacggga gatgacatcg tagttgtttc gccaaaaaac agcggtgttc agcgtgcacg
     7441 tagtctttca gaatatttga acagcacatt agccatcgta gatcatgcag atgatgcttc
     7501 aactgagggt ggttatgtga tcggtgatgt tcaaggaaag acatgtatca tggtagatga
     7561 tatcttgaac acaggagcaa cgcttgcacg agcagcaaat gtattaaaag aaaacggcgc
     7621 aaaagaagta tatgcctgtg cgtcacatgg attattgtcc gatcatgcga aaagtatttt
     7681 agacaatgca ccaatcaaag atatttgtat cactgattct gttttcacag aagaaaatcg
     7741 tcatccagaa aacttaacaa tcgttacatg ttcagaatta atgggtgaag cggtcaaacg
     7801 catccatgaa aatacaccga tgagtccgtt attccgtctg gaagaaaaag aattcgaata
     7861 aacaaaaaaa agagggaaag aagttgtgac agaagtgagc agcttcaagg agctgcgaca
     7921 agacttttgt cacagctcct tttttctatg cccatgaaac gtcctctaga caaaaatgag
     7981 ctcttctttg gtttgttcgc tattttatgc tagaatgtgc ctagcaataa tttggaaggg
     8041 atgaatgaag tgaaaactat ttatctggat catgcggcaa ctacccccat gcatccacaa
     8101 gtaattgaag cgatgacggc aatcatgcaa gacacgtttg gaaatccttc aagcatacat
     8161 ggatttggtc gccacgcgca tgaaaaattg gaaacagcaa gacaaatcat tgcagacagt
     8221 ctgcaagcaa aacctcacga gatcatcttt aacagtgggg gaacagaagg ggataataca
     8281 gctatcttag aaacagcgtt ctcgcgtcaa aaagaaggca gacatctaat cactacagct
     8341 atcgaacatc ctgctgttct taataccatg aaatatttag agacaaaggg gtttgacgtt
     8401 acatatctgc ctgtagatga aaaagggaat ctgtcgatcg aagaggtcaa aaaagcgtta
     8461 cgagaagata cgatcttggt ctctgtgatg tacgggaaca atgaaatcgg aaatttgatg
     8521 ccaatcaaag aaatcggtga acttttagcg gatcatccag cttatttcca tacagatgcg
     8581 gtacaagcgt acggaaacca aacgatccat ccagaagaat taggaatcga tttattaagt
     8641 atctctgctc ataaaatcaa tgggccaaaa ggcgtcggtt tcttatataa acgagacgga
     8701 gtaaatatcc ctgcattgct tttaggcggg gagcaggaag aaaagcggcg tgcaggtaca
     8761 gaaaatttag cagggatctg gggtatggca aaagcagttg aactgctgtc acctgaagaa
     8821 cgtgaagcac gtaacaacaa atataaagga ttccagcaat tgattcttga tacattagaa
     8881 gcaaacgaaa tcccttatca agtaaatggt gatttaacga ataaactccc tcatgtgttg
     8941 aatcttcgct taattggcga agcaaatgat ttgctactga tgaagcttga tcttaatggg
     9001 gtcgctattt ctactggttc agcttgtaca gcaggaaata tcgagccttc acacgtatta
     9061 gaagcaatgt atggagaaga tacaccgata ctaaaagaat ccatccgtat cagctttggt
     9121 ttgggaaata ccgaagacga catccagtat ttccttgatc aattagttcg tgctgtcaaa
     9181 aaataactag tgaagtcatt cttatatgaa cagatgtctg agagaacgcc ttcatttttt
     9241 agggaactta actgaaaaac ctagtaaaaa tcaggataaa cttttataat aaagagaaac
     9301 taattttatc gaggtgaaaa tcatggcatt tgaaacaaca gcaacggtac tgggcgcacc
     9361 aattagctat cgtttgcatc ctgatgcaaa aagatataca ttgcgcgata atggatttac
     9421 agaaacaaac ggaggcaatt atcaattgat ccgaccatta gatgccacgc cgcaaagcaa
     9481 agaaggattc aaattaaaaa tcactgtatc aaaagatatt caaacattga aaatgtcgat
     9541 cactacagca aatggattga aagcagtcaa tattttcaaa gatccaaaac agaaaatgag
     9601 ccaagataaa ttttattttc tgatggatgg gatgattagc cgtgggttat tcgaaaaagt
     9661 cgaatgatct gtggatataa aaagacaact agttttgaaa cggcctattg ttagatgact
     9721 aactttgtaa ggacgacgca aattagttgt cttttttatt tttaaattta acacatgttg
     9781 ttatttttaa tattttgctg atttattgtt tctaagtaaa gaaatttaaa ttgaaacgat
     9841 tgttatttag ggaaaaaggt attttttgaa aaaagaatcc cgtataatga ttgttgtgca
     9901 aacgctatac aaaatgaaaa agaaaagagg gatgtttatg gaaaatgaac agtcggtcgt
     9961 tttaacgaat tggaagagaa attatttgtt ctttttatcg ggacagtttt tgtcaggaat
    10021 cactagtatg gtcgtccaat atgcgatcat ctggtatttg acaagagaaa ccggttcggc
    10081 gacaattttg agttttgcta ctttactagg aatgattccc atggttttac taagcccatt
    10141 tgtaggaccg ctagtggatc gctgggataa aaaagctctt ttgattgtca cggatattat
    10201 tgtagcgatt ttcgcgctta ttttagcagt agtcggaacg atttcagaat ccttcccgat
    10261 ttggctagtt tttgtgtctt tgtttatgcg ttcggtcgca caaacattcc aaatgccaac
    10321 gatccagtcg attatgccga caatcgttcc atcttctcac ataacaagga cgaatggaca
    10381 gttaggaatg gtccaatcag cgaatttcat cattgcgcca gctctaggtg cggctttgtt
    10441 ttcagtcgtt cctgtcaatt accttatttt attagatgta ttgggggcag tttttggtgt
    10501 cggcttattg atttttgtga agatcccaaa agtttctcct gaaatattgg aagttccgct
    10561 tactattttt aaagatgcaa aatttgggct gcagcaattg atggataata aagggttatg
    10621 gtacataacg atcaatggtg catttgtgat gctgttgttc atgccagcaa tcagtctgta
    10681 tccattgatg acgctagatt actttggtgg ttccgttgga caggcgggag cagtggaagt
    10741 agtctacgca gtcggaatgc tcttaggcgg tgcactgatc agttttatag gaacatggaa
    10801 agaccgaatg aaacctatta ttatagccta catcattatg ggcctgacga tcggtgcaag
    10861 cggattggtt ccaaacgata gtcaagggtt cttgtacttt cttatcttga atgcaggagc
    10921 aggttgtgca acgccatatt tcaatacact gctgatggca atgatccagc agagttatga
    10981 atcgaatgtt ttaggtcgtg tcttagggaa cttcaactct ttgatgaacc tggcaggtcc
    11041 aattggatta ttatttgcag gaccattagc tgatcgttta ggggtagaaa aaatgttctt
    11101 gttttccgga atcgggattt tattatgcgg aatcgtttta tttttaacgt cagctgcccg
    11161 aaattatgat aaagaattac agaaaaaact ggtaaaagaa caccatgagc aaaaagacga
    11221 atagtatgga atggagttgt gacagaagtc ttgttacaac tccatttctg atcctaatgt
    11281 tcctgtggca ataagcctac tcaaatgaaa aatatgagta attatcttta tttgtttaat
    11341 acttgatata gggcagtttt ttcacgacct atcgtacttc ctctttgctt catagatttc
    11401 tttgatttgt tgctcagata acggagtgcc aaatggtagt ttttccttta agagttctct
    11461 ttccggtgcg tctacgccta cattgacatc gttctcgata ggtacgctgt aatgatggat
    11521 atgtccatga agattgcgga tagtgtcgtt aggcccgagc aacatcggat aatgagtcaa
    11581 gtaatattgt tgatggtcga atttaacgat ggcacctaca tcataaaaat aaaatttggg
    11641 acttccgtca gtaagcctag gatcattttt ttctaaataa cggaaaaaag cgcgactgtc
    11701 atggttccct ttgatcaagt ggattgttcc ttgcagttga gtgaccagtt ccaaaatttc
    11761 tgcatttgct ttttcatatt gcggatgcat agcaaggtcg cctagatgat aaacatggtc
    11821 ggtttctttt attactgcat tccagctagt gatcaactgt tggttcattt catccacatt
    11881 tgcaaagagc cgcggagcaa agtcgttttt tcctaataag cgttcatgga aaaagtgcat
    11941 atcactgata aagtaattca tgtatctttt gcctcccttt tttcttagag taaacaaaat
    12001 cacttatctg agcaactcat aacatctaaa aagaaaaggt tgtaaatgag acgtgcagct
    12061 ttaagcgata agaagcaatt ttgaaaaata gtttttcaca atttataaaa ttattcctta
    12121 ttgccgaaag gctggccctt tttatgagta tgccaatttt ttatatcgaa tgctcaaaag
    12181 atgagtgatt ttttggttga ctatcatgtt tgtgtactga ataggtccag tagaacagta
    12241 tttttacgac ttgataatta agggatgaaa ttttcagaaa tatgacgtat aatgtaaaat
    12301 actgaaaagt tgcgaccttc aactcgtaga tgtttcagaa tctaattgaa atgatggtga
    12361 tatcatgaca gacaacagca aaacacgcgt tgtcgtaggg atgagcggcg gtgtcgactc
    12421 atctgtaaca gcacttttgt taaaggaaca aggatacgac gtgatcggaa tattcatgaa
    12481 aaattgggat gacacggacg aaaacggtgt atgcacggcc acggaagatt ataaggatgt
    12541 agcgaaagta gctgctcaaa tcgggattcc ttattactct gtgaattttg aaaaagaata
    12601 ttgggatcgt gtatttgaat atttcctagc cgaataccgc gcaggacgta cacctaaccc
    12661 agacgtgatg tgcaacaaag aaatcaaatt caaagcattc ttggattatg caatggatct
    12721 tggcgccgat tatgtagcta ctggacatta tgcgcaagtc actcgcgatg aaaacggcgt
    12781 ggtccacatg cttcgtggag tagacaataa taaagatcaa acttacttcc ttagccaatt
    12841 atcacaagaa cagctttcaa aaacgatgtt ccctctaggc ggcatggaaa aatcagaagt
    12901 acgggcaatt gctgaacgcg caggccttgc gactgctaag aaaaaagatt caacgggaat
    12961 ctgctttatt ggtgaaaaga acttcaaaca atttttgagc aactatctgc cagcaaaaaa
    13021 aggaaatatg gtcactcttg atggtgaagt caaaggacaa catgctggct tgatatatta
    13081 tacgattggt caacgtcaag gactagggat cggtggcgga ggagattcgc aagaaccctg
    13141 gtttgttgta ggaaaagatc tagcgacaaa cacattatat gtggggcaag ggttccatca
    13201 tcctgcattg tatgccacaa gcttggatgc aagtgaaatc cactttacga cgaatgaacc
    13261 aatgccaaaa gaattcaagt gtacagcgaa attccgttat cgtcagcaag acgttcctgt
    13321 gacagttcgc ttattggatg ataatcgtgc agaagtcgta ttcgacgaac ctgtacgtgc
    13381 aatcactcct ggacaagcag ttgtatttta tgatggaatg gaatgtctgg gtggtggttt
    13441 gatcgaccac gcatatcaag aaacaaaagt tttacaatac gtttaagcga tattaaatga
    13501 atcaaaacag ctatggctaa caaaagaaac accttttctt ttggatagca tagctgtttt
    13561 tttgcaataa aattaatttt tattcatttt atacttgaaa tacttgtgta aaaagttcat
    13621 aatatccaat aatatgaata aagttgcttt ttgttaagca agagcagttg caaaatagaa
    13681 gggaatcttt tgcaagtgat ttgttcaatt ttataagtga gggtgatgga aatggaaaag
    13741 aaatcgacgg gtattagcaa atggggactg gttgctctgg ttgtcagttc gtcaatcgga
    13801 agcggtgtgt ttgggatcac aagtgatctg gctagttctg cagctccagg gccagcgatc
    13861 ctttcatgga taatcgttgg gattggtatt ttagctttgg tattatcgct gaatcattta
    13921 ggagaaaagc gtccagatct agatggtggt attttcggtt acgcggaagc ttcatttggt
    13981 aaattaggtg gcttcatcag tggttggggt tactggttgt ctgcgtggct gggaaatgtt
    14041 gcttttgcaa cgatgctgat gagcgccatg ggagaatttt tcccgatatt caaaggtgga
    14101 caaaatgttc cttctatttt atttgcaagt atttttattt ggggtctgac tttattagta
    14161 aacaatggag ttgaaggagc gagcgtcatc aatacaatcg tgacgatctg taaattagtg
    14221 ccattattcc tatttttaat atttgtattt atcgcattta aagtgaatat attcactgct
    14281 gatttttggg gtaacgtatc tgataatcta gtgaatggaa cagggcagca aggaacgatc
    14341 tggttgcaga ttaaaggttg tttgatggtt atgatgtggg tctttgtagg gatcgaaggc
    14401 gcaagtgttt tagccaaccg cgcgaagaaa cgttcagaag cccagcaagc atcgatcatt
    14461 ggtctgcttt gtttattgtt tatctatatc cttgcttcga tcttgccata cggcgtctta
    14521 tctcaagaag agttagcaac tatttcacaa cctgctatgg caaatatttt aaaaggaatc
    14581 gtgggaaatt ggggagcagc gctgatcaat atcggcttga ttatttcgat cattggttct
    14641 tggttgtctt ggacgatgct cccagcagaa acaacgatgt tgatggctcg agacggtctg
    14701 ttgccgaaag gttggggaaa aacgaataaa aaaaatgccc caacttattc gttagttgtt
    14761 actgcaatct tgactaatct attcttattg actttcttag ttactgatta tgcctatcaa
    14821 tttgcctatt ctttatgtac agcggcaatt ttgatttgtt atctacttgt cgggatctat
    14881 caagtccagt tttctttaaa agaaggaatc aaaaaacaag tagtgatcgg tgggattgct
    14941 gtagtttttg aattgatggg catcatttta gcaggatttt cttatgtcct tttatgtagt
    15001 attgcctatc ttcctggttt ttgcttctac tggatcgctt gtcgtgaaac caaacatcaa
    15061 attacgggaa aagaaaaagt aatgattggg ctgatttgtg tggccgcact gctttcaatc
    15121 gggttgcttg ctgtaggctg gatcaagata taagggggaa agaaacatga aaatcgcaaa
    15181 tttcggtgta gaagaatggc taaataaatg ggaaaagaaa gcggtatatg atattgcaca
    15241 aagctcgatc gaagcattga cattagacga actcgttggc ttagatggga ctagcgtaag
    15301 taaatttttc gaaaaacgca aaaacgaacc attggactat ggctggatcg aaggatcaga
    15361 tgttttcaaa caagaagtcg cttctcttta tcaaaccgtc gatccggaaa acatcttaca
    15421 aacgaatgga gcgactggtg ctaatctttt agctctatac gcgttagtgg aagcaggaga
    15481 ccatgttgtt tcgatgcttc ctacttatca gcaattatat gacatcccca aatctttagg
    15541 tgccgctgtt gactttgttc atttgaagga agaagaagat tggcagtttg atttagaaca
    15601 gctagaagcc ttagtcaaac ctgaaacaaa aatcatctgt ttgaatagtg caaacaatcc
    15661 tacaggcaca ttgctagatc ggaaaacttt agaaaaaatc gcagaaattg ctcgtacagt
    15721 agatgcatat gtattgatag atgaagtata tgcgccttta acggataaag gtgaattctt
    15781 gtcgatcgta gatgtctatg aaaaaggtat agcaaccaat tccctatcca aaacctactc
    15841 cattccaggt attcgtattg gctggacagc tactggacct gagttggcag aagtttttcg
    15901 aaagtatcga gactacacga tgatttgcgg cggagtcctg tcagatgatc ttgctgtaca
    15961 tgcattgaag aataaagaaa aaatattggc gcgaaacaaa aagatcatta cagagaacct
    16021 tgtgatttta aaacaatggg tagccaatga accgaaagtt gaacttgtcg ctcccaatta
    16081 cgtctctact tctttcatta aattagccat cgaagaagat gaccagacat tttgtatcaa
    16141 tttattagaa gaaacaggag tactattagt accaggaagt gcctttgatt taccgaagca
    16201 tgcccgacta ggttactgtt gtaaaaaaga aaccttggaa aagggattat tcctcttatc
    16261 tgagttcttg aaaaaacagg cctaaacaaa aacactgcaa ggacagtctg tgacaactgt
    16321 tcaagcagtg tttttcattt acgcttattg tctatgagaa ggagagtgcc ctgaagctat
    16381 ggagactcct gaagcttttt cttctgcagt ttcttgctga tggatctctt gtgtattgtg
    16441 tcgtacaagc aagaagttga ccaaaacaag aagagaagtc gaaagaaaga cgccacgata
    16501 accaaatgca ctggaaacac tggagccgat cataggccct actacgttac cagttgcttg
    16561 gaaagattgg ttataactga agatccgtcc agcagcttga tgaggcgaat attttgtgat
    16621 caatgcttgg acagcaggga gcagacaagc atcagagata ccaatcaaaa aacgtaaggc
    16681 agctaattgc caaacattcg tgacgaatgc cattgggata taaaccagaa tcgcaaaacc
    16741 caaaccaatc gcaagtattc tttcactccc aatcttgtca cctaagcgac caaatcttgg
    16801 agcggctatc aaagtggcaa tcccaggtat cgaagcaatg attccgctga tcaacgtaac
    16861 attcccatgt ccatgcagca attggcgaat gtacagactg atgattggac taatggaatt
    16921 gttggatgct tggatgatca tcgttgtgac gaacataccg atgacgacgt gtggatattt
    16981 caagtctttg aaaatctgtt tggcagaaac catgttttct tttttcactg ggacaaattt
    17041 ttcatggacg aaaaacagac tcaacaagaa gacgaaaaat aagatgatcc ctgtaatgaa
    17101 aaaggtaggg cgatagccaa acgctgaagc agagatcccg ccaagaagcg gtccaagtaa
    17161 tgttcctgta acagaaccag ttgctaaggt tccaagaact tgaccacttt tttcttttgg
    17221 cgtcccagtt gcgacaaggg cagtggcatt actgatataa ccagaaaaaa tcccttgaag
    17281 cagacgcaat gcgactaatt gatacacact agtcacgcaa cccatcaaag aaatgacgat
    17341 tgccattcct aatgaagcac gcaacagcat caattttctt cctttttggt cggctaattt
    17401 tccccaccaa ggagagataa gcgtagtcac caagaaagta gatgaaaaag tcaatccgct
    17461 ccaaaagttc aattgtgacg tactataatt acctaacgta tctatgtaaa gtgacataaa
    17521 aggcatgact aaactaaatc cgataccagc cataaatgtt ccaaaccata agacaaacaa
    17581 gttttgttcc caagtttctc ttttcctaaa taatttttcc tttattcttg cagccaatta
    17641 gactccttct ttctgaaaat tttcatctac agctctttat tataaactac aatatgtagt
    17701 ttatcaatga aagtctcttt tttctctttc tttttcgtgg taaactgaaa gagtgaagga
    17761 tttattttat gagaggaaac atggaaatga aaaaaagaaa tctgtctcaa gagaaaatca
    17821 tcgactgctt tcgagaacta gcagaagaga tggatgttca gcaaataacc tttcagcatt
    17881 tagccaaacg cttggatatc aaggctccct cgctgtataa ccatttcaaa aatattcgtg
    17941 atgtcaaaac agcgctaaca gcgaaacttc tgcaggaact taatgaccag ctgcgacggg
    18001 ctcttgtggg aaaaagtggt caggaagcac tgcagatcta tgcagaaact tatcgatcct
    18061 ttgctttttc aaatcaagca gtgtatgagt tactgatcag tgtaccgcat accaatgaag
    18121 ctgttttatt agatggaatc catgaaacga atcagatcat tttgcagata tttgaggctt
    18181 ttcaattaac gaaagaagaa aaagttcatc gaagcaggga acttcgtagt atgatccacg
    18241 gttatctttc cttacgtttt ttaggatact ttacaaaaga accaacaatc gatccagaag
    18301 acagctattt ttggatgatc aataacttta tcgctacttt gccttaaaca atcagattca
    18361 tttgtaacaa ttttaagatg ttcattaaat gaccctttta taagagaaaa ttcttgcaac
    18421 tttcttgaaa agtgactaag atgagtctag aaaagctgaa agaaggagca cgagtatgta
    18481 tcaagtaatt accatgttcg gggacaatga gccttggtgg ttttttgaag actgggaaga
    18541 agatattgaa gaagaagaaa cattcgagtc tttcgaagaa gcacgacaag cgtacgaaaa
    18601 acaatggaac gaaatccatc aaaattatga atatatcaat gcaaaaagta actttctcag
    18661 cgccttttgg aatgacggag atgagcgctg gtgtgaagag tgtgacgatg atttacagca
    18721 atacaaggga cttgcgttgt tgaaaaatca tcagcccatc acagtagaaa gcagaaagga
    18781 attttatgaa acaactaatt ctagcggaaa aaccaagcgt tgcgaaagac ctaagcaagg
    18841 tgcttggtgc taatcaaaag cacaaaaatt attatgaagg acccaaggtg atcgttactt
    18901 gggcccttgg tcatttatta ggattgaaaa tgccggaaga tctgaataag gaatggcaga
    18961 gctggcaaat ggacacgttg ccaatgatcc ctaaaaaact aggaatcaaa ccacttccta
    19021 aaacaggaca tcaattaaaa gcaatcaagc agctggctca gcgaaaagat gtctcagaag
    19081 cagtcattgc gacagatgcc ggaagagaag gggaacttgt tgctcgctgg atcttagagt
    19141 atgtgcattt caataaacca gtgaagcggt tatggatctc ttctcaaaca gataaagcaa
    19201 tcaaaactgg attcaagcaa ctaagacctg cgaaagaata tgatgcattg tatgaatctg
    19261 ctcttgcccg ggcaaaagcg gactggctgg taggattgaa tgtgaccaga gccttaactg
    19321 tgaagtacgc ggataattta tcagcaggcc gtgtgcagac accgacacta gcattagtga
    19381 gagaccaaga aaagaaaatc gagcagttcc gtccacaaac gtatttcacg atcacgttgc
    19441 aagctggaaa agaacaagcc aaaatgcttc aaaaaaatca atttgcttta aaaacacaag
    19501 aagaagcaca aaaacttgtt caattgatca gtacaagcaa aggaactgtc acagaagtcc
    19561 aagaaaaatt aaaaacggaa tctgcgccat tgccttatga tttaacagag atccaaagag
    19621 aagccaatca acgctatggc tattctgcaa agaaaacatt aagtcttgta caaagtttgt
    19681 atgagaccca taagatcgtt acttatccgc gtacagacag caaatatctg acaaatgaca
    19741 tgaaaagtac gatgaaagaa cgattgcaag cagtcagcga ctttgctcct gaggtcaagc
    19801 agtatttgaa aaacggagcc atcgtccaac agacaaaagt attccaagac agtaaagtga
    19861 ccgatcacca tgcacttttg ccaactgaaa acagagcacg ttatgaaaaa ttaagtaatg
    19921 aagagcgaaa aatctatcaa atgattgtct ctcgtttttt aggactgttt gctcaacccc
    19981 ataaagtcag ccaaacaaaa gttacggttg aatttggtaa agaacgattc atttttcggc
    20041 aaaatcgtgt cgtccaagca ggatggaaag gcgaaactga aactgagacg gaaacagtca
    20101 aatgggaaaa aggaatgaga atcaatcccg atttcgccat caaaaaagaa ctatctgcac
    20161 cgcctaaacc attgtcagaa gcaagcttgc ttggtcaaat ggaaaaatac agtctgggaa
    20221 ccccagctac gcgtgcagag attatcgaga aactaatcaa atctgaactg atggaacgta
    20281 cccctcaagg actgcaagta tctcctaaag gtaagcaatt attagtattg gtaaatccct
    20341 ctttagtaac accagatttg acagaaagct gggaaaaaga ccttgaagct attgctgcag
    20401 gaaagaaaca agcaggaacc ttcttgaatg gaatcgaaaa agaaacaaaa cgcttggtga
    20461 atgagatcaa atcaagcaaa caagaatatc aagatttctc gattacccaa aagaaatgcc
    20521 cagaatgtgg ggccaatctg cgcgagaaaa acacacgtga cggcaaaatc tatgtctgca
    20581 ccaattcaga ttgcaactac cgcagaagaa aagatccaaa agtctctaat catcgatgcc
    20641 cgcaatgtca ccggaaaatg gaaatcatcg aaggtaaaaa cggtgcttat ttccgttgta
    20701 aatacgatgg aacgacagaa aaattgcttg ataaaaaaga gcagaagaag aaaatgacca
    20761 aacatgaaga acgacgcttg ctgaaaaaat atgcacaagc agaagaacca gaagaaagcc
    20821 cattagcagc agcactaaaa gcagcaatga agaagtaata agatatgaaa gaggttgtga
    20881 tagaagtggt cagcttcaag aaataagaag gaatttacga aaattgcttc tcaaattttt
    20941 gtgaaatttc ggcttatttc cgaagaagct acttctggaa caccatttat tcggaaaaaa
    21001 ggactgtgac aagacttttg tcacagtcct ttttcattta aaaaaatgtt tacttcattt
    21061 gacgtatatt attggtgagg cttcaattca tttggcttca ctttttctgt ttctcttatg
    21121 atataaacag gacgctgttt cgtttctaag aagattttcc caatatattt cccgatgatt
    21181 ccaagacata acaattggat tccaccaatg aacaggaaga tcgagaccat ggaaggccat
    21241 cctgatgtag gatcaccaaa caacaatgcc cgcaaaataa tgaacaaaat tgcaatacca
    21301 gaaccgacac aagacaaagc gccgataaat gaagcaatcg ttaacggtgc atccgagaag
    21361 ttcacgatac catcaatcga gtagttgaat aatttccaga aggaccaaga tgtttcgcca
    21421 gccacacgtt cgcggttttc ataagaaata tattctgttt taaatccgac ccagctaaat
    21481 aatccttttg aaaatcggtt gtattctgtt aattctagga cggcatccac catttgacga
    21541 gtcatcaagc ggaaatcacg agcgccatcg accatttctg tatcgctgat tttattgatc
    21601 aactggtaga attttttcgc aaagaaactg cgtatcgcag gctcgccttc acgagtcgaa
    21661 cgccgggtgc cgacacaatc gatatcagaa gtagtgatcc ggttgatcat ctcaggaagc
    21721 agttcgggcg gatcttgcag atcaacatcc atcacggtca ctaaatctcc tgtagaagct
    21781 tgcaatccag cgtacagagc cgcttctttc ccaaaatttc tggaaaatga gacaaagcgg
    21841 acataaggat aagctgaggc tagcttcttg attgctgcta acgtaccgtc ttttgatccg
    21901 tcgtcaataa acaaatattc gaactcatgg gcacctttga atttttcaac ctcatcaaaa
    21961 aaaagaggaa cagttttttc ttcattaaaa catgggacaa caattgatac tttcatcgtt
    22021 tctatccttt caatcgtatg tttatatatg gtttacattc aaaatttaat aaaccttaaa
    22081 taacaccttt aaaatttgat tttacgtaaa agaacaggta ttctttttgt aaactaacgt
    22141 atttagttta ccatgtttcc tcggtttatc aaacttttaa gaacaatttt tattagttgg
    22201 caagtagttt tggatagaga aaaaggagga agttccagtc tattgactag gagcaaacaa
    22261 aatgaaaata cacacaccta tcggcttttt atggttagat gcagatgaat ccggattgac
    22321 tgctgtatct tttcagccaa tttcagaaag cgaatcagcg aatcagcgaa ttttaacgcg
    22381 cacacaagaa cagtttgaag aatattttgc gggaaaaaga aaagaatttg atgttccttt
    22441 atctataaag agaggtacgc cttttcagca aaatgtctgg aaagctttgt cgaccattcc
    22501 ttatggagaa atccgaacgt ataaagaagt agccgttgct gttgatagtc cgaaagctgt
    22561 gcgagcgatt gggcaggcaa atcgaaccaa tccattgcca atcgttattc cttgtcatcg
    22621 agtgatcggg caaaatggaa aactgacagg atatatggga aacgcaatag atggggttga
    22681 aatcaaacgc caattgttgt tgttagaagg atatctgcaa aattaaaaac agttgtgatt
    22741 cggacattgt tccatgcgag aatcacaact gttttttcat gttcattcta aactttgatc
    22801 agccaaataa atagtaaagc catcactgtt tggagaagcc cgacagctaa tccataagat
    22861 aagaagcgtg taccttcttt catgaatttt gcaaagtcta aacgcagacc aattgccgct
    22921 aaagcagtga tctcaaacca gctgctgata aaatgtgccg attggtcaat aactacaggt
    22981 aaagaaaaca agctgttgac caaacaaaag atcacaaacc ctaatacata ccaaggaagc
    23041 ggtaattttc gttttttctg cgtatcagtt ggcgtttcaa acagagctgg ttgtttcttg
    23101 tgcatgaatt tttcaaacag atagacaaca acaacgagca tcataatacg tagaatctta
    23161 aatagcatgg cgaattcgac aacagaattg tcaagtaagc ttgccccagc gactacttga
    23221 ccaacagatt ggagtgtacc acccaaaagt gcactttttt caatgagatt tccaccaaac
    23281 aactgtaatc caagaaaagg gagagtcaac atcatcacag tacccagtag attgactagt
    23341 gtgatgatct gtcctttttc ttcctctttc gcatggatgg aaggggcgat tgaagcaata
    23401 gcagaagaac cacaaacagc gtttcctcca gccatcatca aagaagaagt atcgctgaat
    23461 tgcagttttt taccaatcaa gtaagcaccg atgatagtga ctgacatttg cagtaacaca
    23521 aaaacaattc cttttacacc aatttgagca atcgtttgag ctgtgacagt tgcacctagt
    23581 aaaaaaacag agaattctaa tagtttgctt tcagcaaatt ttgtgccgat tgctagaaca
    23641 ggctgtttaa aaaaagtatt tcctaataaa atacccaata aaatagcaat agtcgctcca
    23701 cctaactgtg gcagataaat agcaagaagc ctgctgaagg cagcgatggc aaaagaaagc
    23761 agtaaaccgg gtaagatttc gcggaccgct gaatccttta cagattttat tggatgtgac
    23821 aatgatttca tttgatcggt tcctttcgtc aataaatact aaaagcatac tcgaacatag
    23881 tagcactaaa ccagtatttt gacaaaagaa caagaggtcg tgacaaaagt cttgtacgac
    23941 ctcttgtttc gaataaacgg tgttccagaa gtagcttcta cggaaataag ccgaaatttc
    24001 acaaaaattt gagaagcaat tttcgtaaat tccttcttat ttcttgaagc tgaccacttc
    24061 ttttacgacc tcttggtaaa cggtgttcaa aagcttgcat ctgcggtaat aagcccaacc
    24121 agtacaaaaa catgaacagc tgtttttgtt tggccgttct tattacttga tgcagaacag
    24181 ctttttcaca acctattgaa tcgattatta agcgattttt ttagaaacaa attttccatg
    24241 tttgctatgt tctttaatta agaaacgtac gtaatgagca agaacgtaga agacaactac
    24301 acttgcaaac agacattccg caacttttaa gaaatccaat acgctactat agatacctaa
    24361 actgttgaag taatgaacag tagtaaatat agctgttgca gagataacaa gcgggaatgt
    24421 aaaggctgca tagcttggat aaaaacgaag cttgaacaaa cgtggaagca tagacagcac
    24481 aacgaaatac aatacttgag ataaagcgaa caatgctacg acaagataaa aattaggttc
    24541 agcaaagttt ttaagataac ctgttaggca aagagaacca ggtgctgcaa cgatcgtaat
    24601 cagcggacgt gtcggatgag ccatatttct tacgaaaaag acacgtttga tgatgatcgg
    24661 caataagaac gcatagcaga tcaatgaaat ccaaaagaaa atttttccaa taagtgggaa
    24721 gaagttcccg gcagttacag tgatgacccc aaatccaacg taaatgataa accagcttgg
    24781 ataaattgct tcaattgaaa gatcagcttt gataacatgg tgataattga aataaaagac
    24841 aaggatcact tggaagataa cagcgcacag ccaaatgaat ttgacaattg gtgcaatact
    24901 ttcatatgaa acaaaataag tacaaataac catgagtgac atcgtaaaag ttggtgaaac
    24961 ggaggcgatg attggatcat gcaacgtttg tttcgtgtgt tcgaataaga taaacaattt
    25021 aacaaaaatg aatatcataa gaatcataga gataccgcca gttagattgc ctaaagcaac
    25081 atggtgatag tctttaaaca gatttccaag ggaagctaac ccaaggatca acccgcagat
    25141 cgggatcgga ataattttca aaaagttctt catatataat attctccttt ttatttaata
    25201 gattttagta ccaagatgaa cgaatacatt gtaaaatgaa taggaatgta tgattacgag
    25261 caagcagaca gctgattgtc ttcatctaag ttactagttg gatcagcaac tgttttgttg
    25321 tacatttcta ttatagatgg ttttttgtat atttaaatca aatagttttt atagtaatta
    25381 tcataaaaag ttatggaagt ggtgccatgt ttaagttatt aacgactttt cgagcagtat
    25441 atgaaacaag aaatttttcc agagcagcag aagtactgtt tttatctcaa ccagcagtct
    25501 cgaatcaaat caaacagttg gagcgggaat tgaatatcga tctttttcaa cgtaacggaa
    25561 gaaaagagat gcttccaact aagcaagccg acttattata tgaacggaca ctggttttgt
    25621 tagaagaatg ggaagaaact cagcaaaaat tgctcaatga acaaaatgaa gaagaagtat
    25681 gtcggatcgt tgcttcccac acctttgctg tctatctttt gcctcagttg atgaaacatc
    25741 tgatcaaaag gtttcctaaa gtgcattttg agatcgaaat cacgaattct catgaatcat
    25801 tagaacaagt agcgaaacat gaatccgatt ttggttttat cgaaaagcca ttagagactt
    25861 ccggcgtaca aagatactct ttgatggaag accagctagt catcgctggg gacccaaaaa
    25921 gcaaattgtg gcttgtaaga gaagcaaact ctggtgtttt tcattacacg cagcgttatt
    25981 ttgaagagat gaatatccaa gataagaaga tgatcgtgaa aagtaacgat gtaattgttg
    26041 cgttgctgaa ggaaggaatc ggtcgctcga ttttgtctaa gcgctctgtt ccagaaaatg
    26101 tgccaatgac tccgttgaat ttccatcgat atttttattt catccaacga accaatctgg
    26161 tttctgatca gctgctagag attgccgaag agatccttcg atacaatgaa agcacagaaa
    26221 cttctcacag cgaggagaat ctcagctggt agataattgc atgtatcaaa aaaagttcta
    26281 tgatacacta aagataagca gtaggctttt gtactgcatt tgctcaagca actgcacaaa
    26341 cggaaagtta atttttccca aatgtctatg gaaagacttt ccattatcta actggaccgc
    26401 tataataatc agcagtatgg tttttaagaa aagggatgaa acatggaaaa aaaattaaaa
    26461 gcttcgaacg ataatcgtat tgattatggc gtgattttac cagtctttct gttatgtttg
    26521 attggtctgc tctcattata tgtagcactt agccatgatc cgaacaatcc agcagtcttg
    26581 aaaggcgtag ggatgcaagt tgtctggtat ttagttggag cggcagcaat cgttgtcatc
    26641 atgcatttga attccaaatg gatctggaaa ctgactcctt acctttatgg tttggggtta
    26701 gtggtaatgg gactcttgct caaattctat gatcagaatt tagctgcttc aacagggtct
    26761 aaaaactggt ttcgattcgg tgcgtttact ttccagccag cagagttgat gaagattgcg
    26821 tacatcttga tgatggcact gatcgtaact aaacataata cgcaagtaaa agaacggacg
    26881 atgaaatcgg acttttggct gattgggaag ctcttgatcg tgacaatacc ggttttagca
    26941 ttgatcatgg cccaagatga ctttggaacg atgcttgtct ttcttgcgat ctttggcggg
    27001 attttcctga tgtctggaat ctcttggcgc atcatcgcac ctgtagtaat cttggcagta
    27061 gtcgttggag caggaacgat cttcttggtt acgactgaag gcggaagaga cttgctttat
    27121 aaagttggat tcaaatctta tcagtttgca cgtattgatt catggctgga ccctttccat
    27181 gatacgtcag gaatgagtta tcagccggcc caaggacttt tggcaattgg taccggtggc
    27241 ttgtttggta aaggattcaa cgtaagcaat atctatgtgc ctgttcgtga gtccgatatg
    27301 atttttaccg ttatcggtga aaacttcggc tttatcggtg gtgcattcgt cattttcttg
    27361 tattttattt taatctatcg tatgattcgg gtgtgtttcg atacgaataa tgaattttac
    27421 gcctatatcg caagcggttt gatcatgatg ctgctgttcc atgtctttga gaatattgga
    27481 gcaaatatcg gattacttcc tttaacaggg atcccattgc ctttcattag tcagggagga
    27541 tcctcgattt tagggaacat gatcggtatc gggttgatat tatcgatgag ataccaaaat
    27601 gaagcacctg caagatcttc aaggaggtaa gaaaattgta caccttttac tggtacccga
    27661 aatgttcaac atgcaaaaaa gccaaagcgt ggttagacca gcatggcgtt agttatcaaa
    27721 ccgttgatat gatcaaagaa gtaccgaagg cctcaacgat tgagacttgg ctagaaacga
    27781 gtcaagtacc aatccgtcgc ctgttcaaca caagcgggat gaaatatcgg gaacttggac
    27841 tgaaagatca agtaggcgag tttacggctt caaaagcgag ccaagtctta tcgacagatg
    27901 gtatgctgat caagcgacca ctgatcttac gagaggacca gcttgttgca attggattca
    27961 atgaaaaaat ttatgaagga gaactgatat aaaatggcag gcaagcattt gaagaaaaag
    28021 gacaaccttt ggatcttatt taatggaaaa gaatactgtg ttggtttgac aaatgaagcg
    28081 caagaagaac ttggagacat cacgtttgcc aatctgccaa aagcaggaca atcgctaaaa
    28141 aaagatgaac ctctgatcga agttgaagcg gaaaaagcgg tcaatgaatt tgccagcccg
    28201 ctaacgggta cagtttcttc tgttaatgat aaaatcggtg aagatatcaa tatcttgaat
    28261 gattcagatg aaatgaatgc gtggatctta agtttcaaag atgttgatcc aaaagaattt
    28321 gacgaattat aaaatcagaa aatcacttga aaaaagttga cactgattct tccagttgct
    28381 ataatcataa gcaatcctat atgaaacgag aaagctttga aaagaagagt acatgtcacg
    28441 gacgtttgaa gagagccctg tttgctgaaa atgggtaacg aaaatgagat ggaagatggt
    28501 cttagagcag agtaagcgag cgtaagcaaa cttactacgg gagtgcccgt tacagcgcga
    28561 cagtatacgg aaagaagcta agtccaagta ctggaagagg ctgttattgc gaagtaacgg
    28621 taaatcaagg tggtaacacg gaagttcaag ctttcgtcct tttgcttaag gcaagagggc
    28681 gaaggctttt ttgtttgaaa aatcataaga gaaagatgtg aatggcatgt cattaatcca
    28741 actaacgaat gtcaaaaaga cattcaatgg gaaaaacgga aacatccaag ccgtcaatga
    28801 cgtttcacta aacgttgaaa aaggggatat ttatggaatc gtcggctatt ctggcgcagg
    28861 aaaaagtaca ttagtccgtc tcctgaatgg acttgagctt cccacgagcg gcgaagtgat
    28921 cgtaaacgat caagatatta caaaattaaa aaacaaagaa ttacgtacat tccgaaagaa
    28981 aatcggtatg atcttccagc atttcaacct gttatggtca agaactgtac ttgaaaatat
    29041 ccagctgccg ctggaattag ctggcgtacc aaagagcaaa cgtaaagaac gggcagaaga
    29101 actgttgcgt ttagtcgggt tagaaggacg cggacaagca tatccttcac aactttccgg
    29161 cggacagaaa caacgcgtcg gtatcgcacg agcattggca aatgatcctg agatcctgct
    29221 ttgtgatgag gctactagcg cattggaccc gcaaacaaca gaagaagtat tagacttatt
    29281 gttagcgatc aataagaaat tgaatttgac catcgtcttg atcacacatg aaatgaatgt
    29341 gatccgcaaa atctgcaaca aagtcgctgt gatggagttg gggaaagtcg tagaagaagg
    29401 agacgtattg actgtcttca gacaaccgaa aaaagaagta accaaacgct ttgtacaaca
    29461 agacatcgaa ccagaagctg attcgacaga actattagct gaactcatca aagaaaatcc
    29521 aagcggccgt ttggcaactt tgacctttag cgaaagaaat gccaacgagc cagtcatttc
    29581 ccaagcgatt cgacgctata acatcgatgt caacgttgtg tatggaaaga tcaaacaagt
    29641 gaaagaaggt tcctttggtt cattgactgt cttgatgact ggtgaaacca aagaattaga
    29701 tcaagcagaa gtgttcatca aagaacaagg agtaggaatt gaggtgatcc atcgtggata
    29761 aaagcttttc agaaacatat ctagatttta aacaaatcaa cccggaaacg atgaatcagg
    29821 ctatccttga tacactatat atgacattaa tctcaatggt cttagtgacg atcatcggat
    29881 tagttttggg attgatcctt tactctattg gacgcaaaaa caaaccgtat gctcgaatca
    29941 tttatggaat cgtatcgatc atcagtaata ttttccggtc aacgccattt atgatcctga
    30001 tggttttgat cattccattt actaaagcaa tcgtaggcac tatgctaggt gcgcaagcag
    30061 ccattccagc tcttgtttta tccgcagcac cattttatgc ccgtctagta gagattgctt
    30121 ttcgtgaagt cagccctggt gtactggaag cagcagatgc aatgggagca agttactggg
    30181 aaatcatctg gaaggtcttg atacctgaaa gtattcccgc attgatttct ggattaacag
    30241 ttacaacgat ctcgatgatc ggatttactg cgatggccgg cgcgatcggc gcaggtggac
    30301 ttggaggtct tgcttggcag gaaggctacc agcgtggtaa tctaacagtg acattcgttg
    30361 cgactttaat tattttacta atcgtattta tcgttcaagg aatcggtgat ttcttaacta
    30421 agaaaacaga caaaagataa taggatatag ggggatgtag gatatgaaaa agaaaatttt
    30481 tggatttgca gcagcgcttt tgctgactgt aggattagca gcatgcggta acaacagcga
    30541 cagcaaagat tcagcaaata aagcagacga cacgacattg aaagtcggcg cttcaccgac
    30601 accgcacgca gagattttgg aacatgtgaa accattattg aaagatgaag gaatcgatct
    30661 tgaaatcgtc aaattcgatg actatgtatt accgaaccaa tctttggaag aaggcgacat
    30721 tgatgccaat tacttccagc atattcctta tttgaacaaa gaaatcaaag aaaaaggata
    30781 tgactttgtc aatgccggtg ctgtccacat cgaaccaatg ggcttgtact ctaaaaaagt
    30841 caaagatgtt tccgaattaa aagatggtgc aacaatcatt acttcaaatt ctgaatcaga
    30901 ctggggacgc atcatcacaa ttctacaaga tgctggctta gtcaaagtca aagatggagt
    30961 agatctggaa acagcaacat tcgatgatat tgaagaaaac cctaagaact tgaagttcaa
    31021 ccatacgatc gacccatcac ttttagcttc tacgtaccaa aatgatgaag gtgacctagt
    31081 tgcaatcaac gcaaacttcg cttacggcgc agggttgaat ccattagaag atgccgtatt
    31141 gcttgaaaag gacaactctc cttacgtgaa tattattgcg actcgtaaag gtgatgaaga
    31201 cagtgacaaa atcaaaaaac tgattgaagt gcttcaccgc aaagaagtcc aagaatggat
    31261 cacagacaaa tggggcggct ctgttaaacc agtaaaggct gatgcaaaat aaggtatgga
    31321 acaaaaaaga tgagaaataa gtctgtaatg gcttatttct catctttttt atacctattc
    31381 taatttagaa atcaaaattg tttaataata aataaaagat atttgatttt agtaaggaag
    31441 gtcattgcgc ttcattctca attagtctaa aatgagtgag aacgaaagag aatttggggg
    31501 tataatgcat gtctgtctta gaaataaaaa atctacatgt gtcgatcgag gaaaaagaaa
    31561 tccttaaagg cgtaaaccta acaatgaaaa ccggagaaat ccatgccatc atgggaccta
    31621 acggcacagg aaaatctacc ttatctgccg ctatcatggg caatccaaac tatgaagtaa
    31681 cagaaggcga aattttgttt gacgggaaaa atgttttaga cttggaagta gatgaacgtg
    31741 cgcgacttgg cttattttta gctatgcaat atccaagtga gataccaggt atcacgaatg
    31801 cggaattcat gcgtgcagcg atcaatgcga aacgtgatga aaacgacaaa atttctgtta
    31861 tggcattttt gaaaaagtta gatgaaaaaa tggctttact aaatatgcca gaagaaatgg
    31921 ctgaacgtta tctgaacgaa ggtttttctg gcggagaaaa gaaacggaat gagatcctgc
    31981 aattgttgat gttagaacct acatttgcaa ttttagacga aatcgattca ggtctggata
    32041 tcgatgcttt gaaagtcgtt gctaaaggag taaatgaaat gcgcggagac aatttcggtg
    32101 cattgatcat cacccactat caacgattat tgaactatat cacacctgat gtcgttcaca
    32161 tcatgatgga aggccgagta gtcatgacag ggaatgctga cctagctaaa cgtctggaag
    32221 cagaaggcta cgctggtatc agtaaagaat taggaatcga ctataaagaa gaagaagcgt
    32281 aagggggagc aagaatgaaa gaaaaaaact ggctcgatta tcttgacgcg gtaaatgatt
    32341 tttctttaag taaaggcgaa cctgattgga tgagaacctt ccgtcaagac gcactagcga
    32401 aagcagatga actgccattg ccgcatatcg atcgggtgaa attccatcgc tggtcgttat
    32461 ttgatgtgaa agaaacacaa accatcagtg aaacaggcac gattccggca tttgatgcaa
    32521 tgaaagacaa tcctgtgctt gtccaacaag gttcatggac gatttttgaa caactgcctg
    32581 tggaattagc agaaaaagga gtcattttta ccgacttgtt cactgcaatg atcgaatatc
    32641 cagaactagt ccaagaatat tatatgaaaa aagcagtaaa catgaacgaa gaccaattga
    32701 ctgcactcca tgtagctttt atgaacagcg gtattttcct gtatgtgcct aaaaatgtcg
    32761 tgatcgatga accactcgag tccttattca ttcaagatgg cgcatccgat gagcacttct
    32821 tcaagcatgt gttgatcgtg gcagatgaac atagtgaatt ttcttatctg gaacgtttcc
    32881 agacaaccaa agaacaagta gcaaaatcat caggtaatat catcgtggaa gtcatcgcta
    32941 aagctggttc aaaaatcaaa tattcagccg tagatcaatt aggagaaaat attacttctt
    33001 atatgaatcg ccgtgggcac attttacgag atgcttctgt cgactgggca attggtgtca
    33061 tgaatgacgg tcatgtgatc gctgactttg actcagattt agcaggagaa ggtgcacacg
    33121 cagaagtcaa aatcgtggca atcagctcgg gcagacaaat ccaaggaatc gatacacgag
    33181 taaccaataa agcccctcat acgatcgggc atattttgca acacggagtg atccgagaaa
    33241 aaggaacatt gacctttaat ggtatcggcc acatcttgaa aggggcaaaa ggtgcagatg
    33301 cccaacaaga aagtcgtgtc ttgatgcttt ctgataaagc acgtggtgat gcgaatccta
    33361 ttcttttgat cgatgaaaac gaagtaactg ctggacatgc tgccagtgta ggacgagttg
    33421 atccagaaga aatgtactat ctgatgagta gaggattgca taaagaagaa gcagagcgct
    33481 tagtgattcg aggattcctc ggttctgtct taacagcaat ccctgtagaa caagttcgga
    33541 aagaattagt cgaagtaatc gaagggaagt tgaatggatg atcgatgcta aaaagctacg
    33601 caatgatttt cctatcttat ttcaagacgt caatgatgaa cctttagttt atctggataa
    33661 tgcagcgacg acccaaaagc caaaacaagt actccaggca ttagcgtact attacgaaca
    33721 cgataacgcc aatgttcatc gcggtgtcca tacgttagca gaacgcgcga cccatgatta
    33781 tgaacaagca agagaaaaag tacgggcgtt catccatgca aaagaaacag cagaagtgct
    33841 gtttactcgt ggtacgacga caagcttgaa ttgggtagcc agaaccttcg gcgaccgcta
    33901 tatcaaagca ggagacgaga tagtgctctc ttacatggaa catcattcta atgttatccc
    33961 gtggcagcag ttggcaaaac gaacaggtgc tgtcttgaaa tatatcgaac taacagaaga
    34021 aggcttcctg gatatggagg atgcagcgaa aaagattacg gacaaaacca aaatcgtctc
    34081 cattgctcat gtatccaatg tcttaggtgt cttcaatcct gttcaagaat tagctgaact
    34141 tgtccatgaa aacggcgggg tcatcgtagt agatggtgct caatctgccc cgcacatggc
    34201 cattgatgtc caagcactag actgtgattt ctttgctttc agcggacaca aaatgtgcgg
    34261 accaacaggt attggtgttt tatatggtaa acgaaaatgg ttagaagaaa tggaacctgt
    34321 tgaatttggt ggcgaaatga tcgattttgt cgaattatac gacagtacat ggaaagaact
    34381 accttggaaa ttcgaagcag ggacaccaaa tatcgccggc gctatcgcat taggccatgc
    34441 aatcgattat ttgcaaaaac tcggtatgga caatatccat cgatatgaag aagaactagc
    34501 cgcttatgtg ttgccaaaat tacaagcaat tgatggattg actgtttatg gtccacaaga
    34561 cccgaatttt cggacaggag tcgttgcatt caatttggat gggttacatc cccatgatgt
    34621 cgcgacagct ttagacatgg aaggggtagc cgtcagagca ggtcatcatt gtgcgcaacc
    34681 gctattgaag taccttgaag tatcagcgac tgctcgcgcg agcttttatt tctacaacac
    34741 aacgcaagac gccgaccgtt tagttgaagc aatcatggca acaaaggagt ttttccaaca
    34801 tgggactatc taaattagac aatttatacc gtcaagtgat cttggatcat tcttcccatc
    34861 cgcatcatcg tggaagcttg actgactcga caagcaagat cgaattgaac aaccccacat
    34921 gtggagatgt gatcgaattg caagtcgatg taaaagacgg aatcatcaaa aatatcgcct
    34981 ttgacggcag tggctgctcc atcagtacgg caagtgcctc gatgatgaca gacgccgtca
    35041 tcggaaaatc aatagaagaa gcagaacatt taactgtaga ctttctacag cttgtccaag
    35101 gaaaagaagt caaagatccc gaccaattag gcgatgctgc tatgcttggt ggtgtagcta
    35161 aatttcccgc tcggatcaaa tgtgcgacac ttgcctggaa agcactagga aaagcaatgg
    35221 aaaacgatgg taccgcagct ttccatcatt accacaagga agaggagtga gaacatgggc
    35281 gtaccagaat tagaagaata taaattcggg tttcacgatg atgtcgaacc ggttttcagt
    35341 acaggagaag gcttgacaga agaagtgatc cgtgagatgt caagaatcaa aggcgaacca
    35401 gaatggatgc ttgaattccg tctgaaatct ctagaaacat ttaacaaaat gccaatgcaa
    35461 acatggggcc ccgacctttc agacatcgat ttcgatgcca tcaaatatta ccaaaaacca
    35521 agtgacaaac ccgctcgtga ctgggaagac gtaccagaaa aaatcaaaga tacatttgaa
    35581 aagattggta taccagaagc tgaacgagct tatctagccg gtgcttccgc acaatatgaa
    35641 tcagaagtcg tgtaccataa tatgaaagat gaattcgaaa agttaggaat cgtctttact
    35701 gacactgact ctgctttgaa agaataccct gaattattca aagagtactt ttcaaaactt
    35761 gtaccgccaa ccgacaacaa attagcagcg ctgaattcag ccgtatggtc aggtgggacc
    35821 tttatttacg tgccaaaagc cgtcaaagtc gatgttccat tacaaacgta cttccggatc
    35881 aatgcggaaa acacggggca attcgaacgt acgctgatca ttgttgacga aggagcaagc
    35941 atccactatg tcgaaggttg tacggcacct acttattcaa gtaacagcct gcatgccgcc
    36001 atcgtagaaa tctttacaag aaaagatgcg tattgccgtt acacaacgat ccaaaactgg
    36061 tcagacaatg tgtacaactt agtaaccaaa cgagcaaaag catacgaagg cgcaaccgtt
    36121 gaatggatcg acggaaacct aggtgcaaaa acaacgatga aatacccaag tgtttactta
    36181 gatggaaaag gtgcacgagg caccatgctc tccatcgcct ttgcaggagc aaaccaaatc
    36241 caagatacgg gtgcaaaaat gatccacaat gcaccaaata catctagctc tatcgtctct
    36301 aaatcaattt caaaagacgg cggagaagta aactatcgag gacaagtaac ctttgccaaa
    36361 aacagtgcgg gttcgatctc tcatatcgaa tgtgacacga tcatcatgga cgaatggtcg
    36421 aaatccgata cgatcccatt caatgagatc cacaacagcc aagtctcttt agaacacgaa
    36481 gccaaagtct caaaaatctc agaagaacaa ctctactatc tgatgagccg aggcctgacc
    36541 gaatctgaag caactgaaat gatcgtcatg ggctttgttg aaccattcac caaagaactt
    36601 ccaatggaat atgcagtcga actcaatcga ttgatcagtt atgagatgga aggcagtgta
    36661 ggataaactt gttataggag ctagaatcct tggtatataa gggttctagc ttttttattt
    36721 tgtctatata ctagaaatga ccagcttttg accagctctt ttactgatga gccatcatat
    36781 cagcaaagtc ttgacctgtt ttcttaggtg cattatttgt aatatgtgca tagatgtcta
    36841 atgtcacttt actattttca tgccctaatc gttgttgaac taccttgaca gattctcctg
    36901 cttctaataa taaactagca tgtgtatgtc taaacccatg aggtgtaata cgaggcaagt
    36961 tatatttttt caaaatatat
//
LOCUS       DAZABD010000019        36883 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000019 DAZABD010000000
VERSION     DAZABD010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36883
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.019"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1404
                     /locus_tag="ACHALG_001069"
     CDS             <1..1404
                     /locus_tag="ACHALG_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFN2586493.1"
                     /translation="IPIQVNTDDLTDNLYLIVETGTVKGVVEEAYPMIVNVPELNGSD
                     DQYFNYDVHVYPKNYFQLNSAELDKKDEDGKALAGTVFELRKDDGSDQVVGTYKTDST
                     GKLTVDGLELGDYYFLETDLGTDANRTTYLLDGKKFKFSVKRGETAKVVLDVTNYKRP
                     VITKVIDKETVDTGEDVQYTLKPSIPGNIGNYEKYVVKDVLGAELTYDSSEVIEGMTL
                     DTDYSIERDGQTTNYVFTESGMDKLQKQFDAGDRFFTILMNAHTNSKAKMDTPLKNDV
                     STIYNNGYEPEDETPVDHAYTIIGDHQFIKTDGDTSKALSGAKFVVKNKIDPNKVTYL
                     KQDPTTKAVTWIADKSKATEFVSGSDGKFQIEGLQYTYDVDFDKQGNPTITDKKINDY
                     ALEEVVAPSGYTPTRNDVPFVVSNDDASSEVTDIKNLKTPETVRTGGIGTVVFLVVAG
                     VAGTGLYVVYKREQHKA"
     gene            1479..2336
                     /locus_tag="ACHALG_001070"
     CDS             1479..2336
                     /locus_tag="ACHALG_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFN2586494.1"
                     /translation="MKKKNIKLIILKIGMLLCLGVGLYPVITQAYTYYQTQQTLNSYK
                     EKYDEASEKENEEVFKRYSEVNRKRKDTEQTSVTIPEDNNNIPSNGNLQKDKLGELVG
                     EILIPKINVDLPIYDGVNDFQLSAGAGILTGTDYPTGGIGNHSVISAHSGIPNKKYFT
                     DLELLKLGDKFFIDISGKKLAYQVDRIKTILPTEFEDLLPVAGKDYVTLMTCTPVGDN
                     SHRLLVRGVSIPYQKSIVEKEKKKEDLKVKAQMNWYPIVVVGVLLIIAVIVIIKKIVA
                     KRRGMKNEE"
     gene            2326..3435
                     /locus_tag="ACHALG_001071"
     CDS             2326..3435
                     /locus_tag="ACHALG_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFN2586495.1"
                     /translation="MKNKILLFLTLICTLVLTIYPANQLYHQFKTKENVAEIQEKIEK
                     KQTPSKEEVAKKVEQIKSNSGNQGLDNTQKTDKGVDNTQETDGGSFDIREAEGKVNPY
                     TGDVFSKNQTKDFEPSTYSGAVGYVYIPKINVLELLFLGASQEHLYRGVAQMSATSLP
                     LSGKGHQTVIAGHRGGYTSPQFLYVDQLVAGDHIYIDYLGEKAVYTVFRTDVIRNYGQ
                     NSTLTDIPDLDMLTLYTCHPYPQNYDRLLVRSLRDEKAKYITPQVTVGNTAYADAIDS
                     ESVSDKPETVTSSNDLFEDVDRANKELDGNNDLNIPEQGSVEMQDRTFAEKASSIFTG
                     ITNKHQKMLLVIWTVLGALASLKVLILLIKEFRKA"
     gene            3774..4574
                     /locus_tag="ACHALG_001072"
     CDS             3774..4574
                     /locus_tag="ACHALG_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2586496.1"
                     /translation="MVEISKTALDSLAKLFSSRFDGAEFVEYLSQNESGKTLIETLKK
                     DYLNENDFPTWTNIEDATWDVVCTIYSPWVTIADFVLDKIGGDTYPDAKSLATSDFKT
                     FYGYLMGLIGNGIDLKVTPKQTPEEIEKEKGEVQMAYADDREKVKLLEGKLSEEELGK
                     MVQEINEDSNYFPELKVYQNDEDFFNEMFSNSPWEAVSKTNLGEWNFQDDLVAFSNDG
                     YLRSYSESEYRAELKTYETDILMSYLNLLESGQGELRDKVEELLKEEL"
     gene            4680..5468
                     /locus_tag="ACHALG_001073"
     CDS             4680..5468
                     /locus_tag="ACHALG_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2586497.1"
                     /translation="MSATTDMALEAVIKDNTGNVENVEDFSDIAINLSAWISAVGEEN
                     IEFEIKNMDDLKAKFPFIASKLSQFAEEKSWNELVSFSEKIYDITQEDECVSWEMNNQ
                     YKKIDAFVKSRIEGTAFEFEGIDTADNWLNKESFIKSIADDLEKQWSKEDYAEYLKTE
                     RDTEVKEKLTEVCLDEHIDPNIVKQEDYEVVIDKVPTDFEEVVKYIVEEALSQYSSDE
                     EVDEETVRTRVWQLVFDDKDYKYLSDQNSVNWSVRLLIDQNDIE"
     gene            5700..6569
                     /locus_tag="ACHALG_001074"
     CDS             5700..6569
                     /locus_tag="ACHALG_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFN2586498.1"
                     /translation="MKKKKFSRSLLGILVLVAVGLSGKGYFQNNSTQEHDFQIKSSVQ
                     VGNKADSESKEFLGIRYDPNKYPNNIVNINDGKYTLTKEDHEALSEKGSQTFWVNYAD
                     LDQLGRAGDVEALITYDAINSHSSKNVERPSFKSSVHIAGEYKDGVFDQERDTWVGKD
                     SNNEITQLEGYKGYLYNKSHSLAWSLGGDMEVHNLTLGTRAQNVGKNDQNGGMAYSET
                     IVRDAIYKNKNEKVLYKVDPIYKGNELVPRGSHVEAFSVNDNGKTVNLNVYVFNNQKG
                     ININYQNGTWVRD"
     gene            6589..6936
                     /locus_tag="ACHALG_001075"
     CDS             6589..6936
                     /locus_tag="ACHALG_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2586499.1"
                     /translation="METISINGLDKSVANRIKKGNGYYLLKKGKYSVARELETMSIVY
                     VAKNHFDVEQAIFDPDSEIGKCVELSEKTVSTKGAMIIEREMLDEIRVIIPKDTQMCE
                     DSISDDEPKSERK"
     gene            6949..8211
                     /locus_tag="ACHALG_001076"
     CDS             6949..8211
                     /locus_tag="ACHALG_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2586500.1"
                     /translation="MSEKSIELKEYKRSDGEKYYPVASFSKSVYLVGRALVVGAIGIY
                     ILHWVTKVVQLLVDTLVSILVSLNDVNGTDDLGLKEMFKELLNAFFPNYLSYSYNVQS
                     FFAKLHLVDISILVLEAGTVFFVGYLVTLMFRHHYGEQRPFFDDMESRRLKREALEAM
                     DVKYDPNKIIGDETNNKKRKRNKTQLESDVLSTLKNIKISVHTRRDLSVNEDIREYIV
                     KIPQPKGKKVRETVLRQIQDLGSILTRATSGRAKFGEKEDEVSGDYFIFRASVNIERE
                     RKERVSTKNKETERATSSIAVSNSQFSFPINLFADNSKNIQNQTEKARKYAETQKQSL
                     ELYLSSADVPASLTDMHVGNTSIEYLFTLSRTRKIQLSSMKEEIETYLHISGVIVRIN
                     AGRIQITLPLGENDRIPIDVPSMINSVF"
     gene            8291..9667
                     /locus_tag="ACHALG_001077"
     CDS             8291..9667
                     /locus_tag="ACHALG_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFN2586501.1"
                     /translation="MAMKLLEKVKKRAEKAESILLDKEPNIKLNGKFSVNMLNTKAKD
                     PTDAIFGIKPDGTIAHFCISEMPHMLVAGTTGSGKSVLLNEILVTAMCHSTPDELKIG
                     IVDPKRVEFGRYKKLPFMLADPITDMDEAYDFFEYLVILMHERYKLMEKAGLQNITLY
                     NEYAEKNGLDRFPYVILLVDEYSQLVGTHKEVEGLIVQLGQMARAAGIHVILATQSPR
                     STVVTGIIKANFPSRVCLMVASDLESRIVLDEGGGESLSPKGDMIIKLVNGSMVRAQG
                     AYISNKEIETIFNHLRNTMPEPEYVDYRAIVAESRGEGGEDEDFGGIGKAPVRKSAKR
                     NLNTPLANSSSKEPSIEKKSTFALKLEKNKEEKEKQGAKTLEKLQAEKLEKVLKALDK
                     ADSEPQTSNNTNNEQPISSKTKPKTTDPKVSVNVTITTDKSEKKQVNGTTSPIMQKML
                     KAKKQVHK"
     gene            9664..10293
                     /locus_tag="ACHALG_001078"
     CDS             9664..10293
                     /locus_tag="ACHALG_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2586502.1"
                     /translation="MIIVSSFLRWLTPYKYTDEVVVKRTKKRDKKAVLTPKQMLDHGI
                     LGVKVLEVNKPFESNSAREKLLGAEADVAYFYHDKSKHQIAIKFNPKSAWRYQARDDL
                     KRRKLRPMLYPEGKEAFDRTHIVPIGFHGTESDNRLLVGWNSNQNRVQINKFEKKVRK
                     FNDRKTILWFADICKQPDGTAKWEAVIFDENGKKVMSETWHDKAKFVWQ"
     gene            10312..10791
                     /locus_tag="ACHALG_001079"
     CDS             10312..10791
                     /locus_tag="ACHALG_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2586503.1"
                     /translation="MLELFEANRTGFNQKLEVGETIEYKDKNYIIIGIYNTRINRIGD
                     PRITSDLVCQSVNYSPDLTSRYKKYVLLEYRHKITDEIGVNNTRNIFKIGTVIPYSNN
                     EEKIFYQIYGIEKFEYEHVDLLVTYQMRLIEPWSQAEIDKAVKLNRLSKFNVLGNAF"
     gene            11350..11949
                     /locus_tag="ACHALG_001080"
     CDS             11350..11949
                     /locus_tag="ACHALG_001080"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFN2586504.1"
                     /translation="MREKNKNSSRYLVATVVFVCVLIFLFDFLQIGKSIIGNTLSAKE
                     MVALGGLYEGHLFPNIFISIVSHINLYHLISNLLFLVVLEEALNDQYEWLTLLIVLII
                     SGFLGSLVPFLLHSNIVSMGLSGGIFGWIALFAIAYIRFDSKKHLLLSVFVLLIIGIL
                     LVSTFESGETNVLSHVIGFVCGMILGFILKNVSLIKQKN"
     gene            11965..12441
                     /locus_tag="ACHALG_001081"
     CDS             11965..12441
                     /locus_tag="ACHALG_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2586505.1"
                     /translation="MPKKKEKKIPVFLSVSEMLSLQILLRKKIETIDEEVRNKRAMYE
                     QREALRERIHYFDDIHTKIYEARTNHEDESKLGLAQQRIFEWLNTNTEQEPLRAISNL
                     FEFGTKHWFNAVEKDADLQEAFDTLSEDSKSMRDLLITYLNEHEFEQTKVKKKNKK"
     gene            12458..13360
                     /locus_tag="ACHALG_001082"
     CDS             12458..13360
                     /locus_tag="ACHALG_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2586506.1"
                     /translation="MLFTLLGYLCLVLAFFGAIAIFVGQFQENNPIMRNIGIGIVIIS
                     LIVFGATKIFGSNDQQFGISGVDNSYAPDSLGRVVIDGTFENVTSYKNVSLTVKEDDK
                     KASDDVLATIKPSQSQFEVFYTVKENNPVRKLTLVFNVDGEEYYKDVKINKASTKKIV
                     ESAKKEVATEESTTESSSTVEVELPDKIDFDPEKVKSFESVSGSMADRFNTEVFKAIK
                     TVKLDTEKQRINVTLFNAVESMDQDKKVTVARELGKRVIGLYRGTAKPDSDGSDLPVH
                     VVYEDGKVFGDSTANEALTIKLAK"
     gene            13433..13627
                     /locus_tag="ACHALG_001083"
     CDS             13433..13627
                     /locus_tag="ACHALG_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2586507.1"
                     /translation="MVVNKEKVRKYVTLKIASVEYLEKRAKEEEKTRGKKVTVSELLE
                     ELIESDQTIRRAFTNRSVKS"
     gene            13732..14160
                     /locus_tag="ACHALG_001084"
     CDS             13732..14160
                     /locus_tag="ACHALG_001084"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2586508.1"
                     /translation="MIIQKFDWKMKLGISLVILGFIIFLGIKAQKNQSTFETMSSQII
                     EECSKIYYCENKTEESNGFYYIDQPHDVVIKMESHEVNEWIQRSTNVSKKLERYSQTF
                     LSKVIFSNCKNEETSQRVASSLENLKFSVSKKEKFVDLRK"
     gene            14396..14983
                     /locus_tag="ACHALG_001085"
     CDS             14396..14983
                     /locus_tag="ACHALG_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2586509.1"
                     /translation="MENNNDIRYFSIGERVTTVSELSKDYRDKEGTIISLKKNRRGVW
                     QYVVDLGDGVTVECKDSQLIRNDGSHSIEKAILVFPKVSSKIMNDWLLGKFGEYNKKE
                     IAEVIGKSYPTVLGYLKKITNKEAKVEDVLTVRELQSLIEYRNKQITNPEYLKKEDGL
                     LKQLEQVNKIRKMQKVAPLSLDNFKKIYLKEIEKD"
     gene            14983..15882
                     /locus_tag="ACHALG_001086"
     CDS             14983..15882
                     /locus_tag="ACHALG_001086"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0000400 - four-way junction DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFN2586510.1"
                     /translation="MWSVTLPFQPVSAPRPSARRNVADATNDVAEKRISTYYDKKYLD
                     YLKKIKSFIEDQGLLDDEFYSIVNDPMGAIMEVDFYYQIPKSYKKVYNIMKTTAPDLD
                     NLVKSAMDGIFNISAIRDSHITGLIAFKYNVANEGKTVVRIKRYSEFEWDTSEGLNGD
                     IWEATFDFKPVATPRPKSKFRGNGIITYYPKEYNDYLENIKNTLKEKDLVNDQLKKVM
                     NAPMGVIAQIDYFYQVRKDKKKLDSLMRTSQPDIDNLVKGTLDGIFNKEIGIKDSRVV
                     GLIAFKYNTFEKSKTNVRLQEMG"
     gene            15882..16796
                     /locus_tag="ACHALG_001087"
     CDS             15882..16796
                     /locus_tag="ACHALG_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2586511.1"
                     /translation="MNNNEKILHVLDSFETIQEELKKYRDVLEQRYDFVNQQKSNHMD
                     FILNMNDLKKKLVERKEQEKLIKAYFELGEKEVKKAMELNEDRRVLDQLLEQLLVMFQ
                     KGRIDEDLIEEGLRKYPANSGIGIVLKAIDEEEIEDFIPAEDFESAMEYIKYYSQGIT
                     AFREFDPEDVIHDLNNLKEWCEGYEVDDSGLDYLISIMEIEEEMPDKPDPTDILELIH
                     DARNPIAYISRGYTVLEYYKPYISAMNHLRRVLREKREYRSVLNASNRLEKAVSELDA
                     YYREHYLQAGGMPRNTKANISRYIKEAE"
     gene            16848..17063
                     /locus_tag="ACHALG_001088"
     CDS             16848..17063
                     /locus_tag="ACHALG_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2586512.1"
                     /translation="MKRDVFELYVLERLEDGMMAQEIIDEIDDMISRFGQLELNDGNK
                     NSVVLQQFWLNNFSYKDFIKEKANRLY"
     gene            17253..17507
                     /locus_tag="ACHALG_001089"
     CDS             17253..17507
                     /locus_tag="ACHALG_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2586513.1"
                     /translation="MKKEELIDMFQIVERANNMGIMFFDRISLKMDLSVAHQEFNLRL
                     KALLISDDVNFAHDVVGIQNHIDRENKRMGDGFLPRYSSL"
     gene            17719..18735
                     /locus_tag="ACHALG_001090"
     CDS             17719..18735
                     /locus_tag="ACHALG_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFN2586514.1"
                     /translation="MGLVLLSPSVSAESSISMYRMYNPNSGEHLYTRSVGERDNLKRY
                     GWHYEGVAWQAPTSGSPVYRLYNRYNGEHFYTVDAGERDKISHTGWTYEGVAFYSYNG
                     ANRVPVTRLYNKYVNWHHYTLDQNEKNVISKQGWKIEGTGWYAMPNSAPNPTPAPAPS
                     NEKVLNVPVIWQGNTMLCEGASLLEGLHYKGVATNQNLTSFVNYMPRANDNNPYHGYS
                     GEWRYNVNGTYQGMMPDPVVKWAKNVGGNAVNITGSGTNGIKNEIRNGNPVVTWITYN
                     YASPQFKQMPWGRAVWNGHVVLVDGFKNGAYHVVDPVFGAKWINASTYERAFNVTGMA
                     VAVR"
     gene            18912..19142
                     /locus_tag="ACHALG_001091"
     CDS             18912..19142
                     /locus_tag="ACHALG_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2586515.1"
                     /translation="MNQRRRKRLEEISERLSELKDELEVIQEEEEEYKENIPENLQGS
                     ERYEKAEESVDQLEEAVDGLSDILELIEDIAS"
     gene            19178..19402
                     /locus_tag="ACHALG_001092"
     CDS             19178..19402
                     /locus_tag="ACHALG_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2586516.1"
                     /translation="MIDISLIEKLLFSNKYTAKELATRWQISESAIKAYRQNKGSVGW
                     RDWKGISIGKAEDIMKREGFQADNDSKKVE"
     gene            19619..20494
                     /locus_tag="ACHALG_001093"
     CDS             19619..20494
                     /locus_tag="ACHALG_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2586517.1"
                     /translation="MTTNKRWSELVQNTVKKVMPDGYHFNYIQCSQTTESIYFSILNE
                     NRLYYFRVSSHHRKKREDNVTTFLSGKYNGFKELSEAIGEYLKSNRFVRISDKHILIL
                     LLYLNNSFKLFWRDDTFYYIENGMDIYSGINGILKHYIKALLRYGVLYQEDLSNQIKI
                     SNSGKSLLAKYKKLLSPIYKGIDMEAVNSETLFNLVVDEKFANEFFKSKKIERLNNLF
                     PENAIKAYVDNNKSRLYNWFIPKRLRGKIKIGDQVKVINLNHTKIVTVTELYIAEKED
                     ITKMKPVIKILDNKE"
     gene            20504..21298
                     /locus_tag="ACHALG_001094"
     CDS             20504..21298
                     /locus_tag="ACHALG_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2586518.1"
                     /translation="MVAKKNTLKEASEKYQDMKRRGLIPKDKKGIWREFVKEVSSDRS
                     GGDFVGRYTAKGYSRRLRITVEYGTNESATELKQRYLDTRREASKKYDDFKSRGLIPK
                     KKKGIWREFFKEEDSNRNKGDFIGRYTAKGYTKNLGIKVEYGTGESSWELRNRYLNTL
                     LEKYKAELLELFDVNDFIEMAEKYGHEEGFMINDIMVIDNFTDLLALAKKARDLRETQ
                     MYLFRNKNGRNRRTESSEIEDYYDAFTHDLGAFVDEISGVMNKYRV"
     gene            21486..22445
                     /locus_tag="ACHALG_001095"
     CDS             21486..22445
                     /locus_tag="ACHALG_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2586519.1"
                     /translation="MGYDKPEDAPFFVPSSAYRASTSGSMYNLDLFEEGAKMIEYDFN
                     EAYSRIMRNYPLPSNTYLKAKYNTDQAILRAINRPKVRPYENLKTYMFFLVNFEGTAK
                     EGTYANPKSQSMFADYGENPKTANEPIWLSEIELTLILDYYDIKEMEVLDSHTFLTKK
                     GLLKEYFERIEPLKTDPDTLKLYKNLRTKVFGAIGQKSLREGETSTFDKYSMYNRAYS
                     SMVAGVFRDRMVRYEQKYVHSAYGLLKIRTDGLYFRSEVPEFEELYKLGVVKKKVHTI
                     SKEEVEKALEFKNKGFENKGSFGKSKDEVKGVVIDDDFEFDFD"
     gene            22478..22798
                     /locus_tag="ACHALG_001096"
     CDS             22478..22798
                     /locus_tag="ACHALG_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2586520.1"
                     /translation="MKKKIVEGNVGVIILKDSTDYAKSMALKDEKVARMDKDLVEFLM
                     DDTERLKGDQFLSLMKDKGLNTYGLFDYKKLAIEWVKEGEIFFIEEWEGTENLVLHQY
                     LLKA"
     gene            23053..23742
                     /locus_tag="ACHALG_001097"
     CDS             23053..23742
                     /locus_tag="ACHALG_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HFN2586521.1"
                     /translation="MRTHYIYLEDKIVDEIPAKSREQARKLFIEKYKEINGDSEVPKV
                     VVTVRKRQTKKRSTFHVSPDIYNLIERHRSEDNISISQYIATVIDELISGKYDYAFDF
                     EGEKDYKSVAVFQSDFEKLKNYAGRRNVNFARLLEEFYSEELGVGNEYKRETYAKLSH
                     KLSQALKEVTEVKAELDAKLEEVKKYQTERKNNMKNQVEFDEQKEFEKARKRYNKIMN
                     ELSYEHLTVHE"
     gene            23743..23946
                     /locus_tag="ACHALG_001098"
     CDS             23743..23946
                     /locus_tag="ACHALG_001098"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2586522.1"
                     /translation="MSEGTEKYTIADMVNEVETLLASFFENGHVNDEWRKEEPKRVNA
                     YIARLKRFIDKYKENSDKFSKDK"
     gene            23966..25594
                     /locus_tag="ACHALG_001099"
     CDS             23966..25594
                     /locus_tag="ACHALG_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2586523.1"
                     /translation="MPEIEGIFSPKIGKKQLDFSSFPSVNRDYNLGDEVQILNIDFKE
                     LDFAVAVSNKPKALERVKKIYQENDQNIKNMIKKYRRDNSKVEDVLFNGTAKHDLNVY
                     MALGVALYCNSINQTDILVNIVRNSYQKILKISSETNPYYAMKYLGDNLLNDKKLMHN
                     TEELLSNLAAINFLFKTGSEDYFIDNVLKGAYGTAFYPLYNMKQHKAEQKDNKLEIKK
                     MKELLGIGFVKPNSKVTIASLATTNNDGKIVNGFDDSDIDILSKSYFSLYGDFADLMG
                     IDSYLVNNFEIKGSELNDIVYFVLISLYAIDQNTEYRQIDYLLVFYSLLYQFVLTRLF
                     KNAKEELILNSDYEIAKQIASIRKENKSEVIKLRKELSIASKENGRLESLVKSAKKEE
                     ELLKQEIKELKGIRKELEQLRFENKVLKQLQEQEHRQSLNGKTEEPSLSEMIAYLKHK
                     NILLVGGHVNFQNKLTELLPNIKMFDVSENNGKILKNLNTCDAIFLYTDYLNHGLYYR
                     VMSEISNKDKIFYLDNHVNMNLTIKRIYDLLSLI"
     gene            25645..26016
                     /locus_tag="ACHALG_001100"
     CDS             25645..26016
                     /locus_tag="ACHALG_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2586524.1"
                     /translation="MRELTLSSKELEKKTALLNEIFSADCIIQSLKESSFSKLTDQEK
                     ISYMLSLDNLGTKYDESASSDKLLIELVSSLSSFGKMKRFNVILEIFAWLIPSFIWFF
                     YIFIVESELIAKVSLGLLIPI"
     gene            26101..26301
                     /locus_tag="ACHALG_001101"
     CDS             26101..26301
                     /locus_tag="ACHALG_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2586525.1"
                     /translation="MLKRIAWDKDNKTDIELKEYKYNYFSRCIDRKLLFKKEGLEFSF
                     DEDEFEKLVMDWRKNLLDTVQF"
     gene            26435..27661
                     /locus_tag="ACHALG_001102"
     CDS             26435..27661
                     /locus_tag="ACHALG_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2586526.1"
                     /translation="MIFYMFININEVLKLTTITMTKKIEVYVDGSQEERDSFFDFFWK
                     NSREYVKALNLVYKQLMFEELIKDAIKEMDQGYAEREESIQNRIEKLAYTDPIDQKKL
                     DKAKAELSKLRNEKNKEAREILTQAIGLKSQTRTRDVAKKLDFDFGDFVDSANMKASK
                     DFGNDFIGIVTGQRTPRIYKNRNKYGLLPFRGRDIKIHKDDKFYIYLPKGFRFKVNLG
                     SKPRKARDVASVLQHIMDGDYKLNQSNLIFDGRKLFFNLTISFEKSYNEMEFKKGNEL
                     IVNFADLCSGECLINDKSVMSFGDSQYIRNFKSKILAMKSKEQSRGVFSKGGHGRKRK
                     LDQDRWEAIKKRESNFVKTYNNQCSAKIVKTAIRNKAQIIRIIEPDKAYGEWAYYQLK
                     EQLVNKGKRYGIEVRS"
     gene            28511..29836
                     /locus_tag="ACHALG_001103"
     CDS             28511..29836
                     /locus_tag="ACHALG_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFN2586527.1"
                     /translation="MFDYSREPVNDYFLIDMKSFYASVECIERNLDPMTTPLIVMSRS
                     DNTGSGLTLASSPTAKKKYHITNVSRATDLPYPLPKELHIVPPRMNLYIKKNMEVNQI
                     FRKYVADEDLLIYSIDESILKVTKSLNLFTSSGTRSERRRELAKIIKDEIQKETGLIA
                     TVGIGDNPLLAKLALDNEAKSSKEFIAEWTYETVPEKIWKINEMTDFWGIGSRMKKRL
                     NHMGIMSIEDLANWNPYTIKSQLGVIGLQLYFHTNGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPKDYTRREEVELVVKEMAEQVAIRIRQHNCKTGCVRLAIGTSILEEKSGFSHQMKI
                     PITDNTKELQNYCLFLFNKFYEGQEVRHIGVTYSRLVYTDSLQLDLFSDPRKKIAEEN
                     LDRIIDKIRQKYGFTAIIHASSMLEGARSITRSTLIGGHAGGLGGLHND"
     gene            29829..30176
                     /locus_tag="ACHALG_001104"
     CDS             29829..30176
                     /locus_tag="ACHALG_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2586528.1"
                     /translation="MIEYNDRGRLKWSGFYLSDHTERINADNVQRANHNLAKEQMTTE
                     EIAKILRTAVIKNRPVCIQKEERNAEGYYPDDVVGKIIGYDELGVFVEEEKVHYDEIR
                     NVSFSELLKWSQI"
     gene            30173..30355
                     /locus_tag="ACHALG_001105"
     CDS             30173..30355
                     /locus_tag="ACHALG_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2586529.1"
                     /translation="MNIIKKYEFGYITYEQLIEELWGYGQQLINEVGIDCFCFYIESG
                     AGYHKNRYYIFPYPPK"
     gene            30491..31348
                     /locus_tag="ACHALG_001106"
     CDS             30491..31348
                     /locus_tag="ACHALG_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFN2586530.1"
                     /translation="MEEKLIKKDEIIMGLLVPRANTYTIPLTKRQLIAKRYQRITRAI
                     NREFWNSESDTAHSLYVGSYGRGTAISTSDIDIIVELPMAEFDRFKNYLSNGPSKLLQ
                     VIKNAFQEILPNSDIRADGQVVKINFHDGIKFEIVPAFNEKDYWGESKGFIYPDSNMG
                     GNWKATNPKKEQEAMKLKNTKSNNLLYATCKHFRHVRDTEFTSYHLSGIVIDSFVYEA
                     MGNWKFVENNSGGQNISSVSYETALLEYYNSHKVMGGLNLYSPGSNQFVNSDSSIICL
                     EKVLKKIAL"
     gene            31368..31928
                     /locus_tag="ACHALG_001107"
     CDS             31368..31928
                     /locus_tag="ACHALG_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLATT domain-containing protein"
                     /protein_id="HFN2586531.1"
                     /translation="MGDEKKRLEVQLREAYGRVTYTYTTHLKQADNIISDNKILRCLQ
                     IGLSALTTGGVLSTIITDNSELTFVAGLCSTISLGLNLYFKNFDLAEEAKNHQIVADK
                     LWLIREEYVSLLTDLNRLSVEDIVKKRDQLMNETHEAYKNAPKTNSKSYESAQKALKS
                     EEEQFFTEEELDKMLPEHLRKKSSKY"
     gene            32971..33738
                     /locus_tag="ACHALG_001108"
     CDS             32971..33738
                     /locus_tag="ACHALG_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HFN2586532.1"
                     /translation="MRDRILEVNQEMVNKLTNISGNYEKYDGTLLDNYIFHDTQNIRI
                     GRSKPRAFILIKEKYLNEWNSTYEMVMTDNFEKVLDFRNALLGSDMGNSSLESIEVEN
                     KTENEGEGLIKNVLKIEQKYISEDRSFYRVTSSVGLANFATNLIGNNASESLLVMCLN
                     TKNEVIAYSEVFKGGINQSVAIPREIFQLALLNNSAKIAVSHNHPSGYEFPSENDKSF
                     TTNLKNAGKTIGIELLDHIIVTSKNTSYYSFQENGSL"
     gene            33764..34309
                     /locus_tag="ACHALG_001109"
     CDS             33764..34309
                     /locus_tag="ACHALG_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2586533.1"
                     /translation="MGERTQALITVKYGNSKTKASVSIHYQWGYGRYMLMDVLNQGIY
                     LINKAYDLRDKSREELAKGIFDRSTGVINFVTYESGVSAYDETGFGGRYEPYQETIED
                     VMKFSDNNDGYAHLEMVFDENGFSLDDKTRLRLFDFEGQQIGLNKFCESGKEFATKDF
                     IKGYRLLLENYGINLEEESVK"
     gene            34310..34876
                     /locus_tag="ACHALG_001110"
     CDS             34310..34876
                     /locus_tag="ACHALG_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2586534.1"
                     /translation="MFEKRKDQFPNLEAKSQKVIDGEMIELFQKSYGTIETTASCKYY
                     FQGKRNTFKGFADSDEELAKKIIDWLVSSEHVAREDTVRTTINGEVLTGTRCVCCNHE
                     FVEDEGCYRINEYGDFICQSCHEIEFPTERSWFEYMLQDYEMEKENGEVVFCTKEELS
                     KRSDDEIEDWCVWLVGADDCVAYYTTAE"
     gene            34891..35139
                     /locus_tag="ACHALG_001111"
     CDS             34891..35139
                     /locus_tag="ACHALG_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2586535.1"
                     /translation="MEGMDFLDHEDLVDFGYTWKGMVGISRSLANAFYERNYAVYVLY
                     DDDTESLVDEEYKLDLENVLYGIEKEDLAKYIFSWLGQ"
     gene            35159..35398
                     /locus_tag="ACHALG_001112"
     CDS             35159..35398
                     /locus_tag="ACHALG_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2586536.1"
                     /translation="MTKFKSKGMQWCSYYYVGNDEDLLRKTQRKGYPDKILKQIGYYM
                     PEERIEKEFCEVLFDSEQLLVLLTQNKLICQFENN"
     gene            35769..36050
                     /locus_tag="ACHALG_001113"
     CDS             35769..36050
                     /locus_tag="ACHALG_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFN2586537.1"
                     /translation="MKVKNPTEKSRIQVGIDKNLKENAEMILEELGLNPTTAITILYK
                     QVVARGEFPVEIKLSEEEKQGIRLQQLTKDMPVDVLDTDEKLEEWFDEA"
     gene            36040..36381
                     /locus_tag="ACHALG_001114"
     CDS             36040..36381
                     /locus_tag="ACHALG_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFN2586538.1"
                     /translation="MKPNEIVTVYVAFTDKNGGKRRPILVVSDKEDRVEFFGITSQYE
                     KKSDQIKRVYFPIAEWEKAGLKKQSWIDVGSLKAIPKSRENISFKKVGMLSASDTKKL
                     NRFIEKLQRKV"
BASE COUNT        14084 a         4873 c         7382 g        10544 t
ORIGIN      
        1 atacctatac aagtaaatac agacgattta acagataact tgtatttgat tgttgaaaca
       61 ggaacagtca aaggtgtagt tgaagaagcg tatcctatga ttgttaacgt acctgaacta
      121 aacggatcag atgatcaata ctttaattat gatgtacatg tctatcctaa aaactatttc
      181 caattaaact cagcagagct tgataaaaaa gacgaagatg gaaaggctct tgccggaact
      241 gtttttgaat tgcgaaaaga tgatggatct gatcaggttg ttggaactta taaaacagac
      301 agcacaggta aattaactgt ggatggtttg gaattaggtg actattattt cctagaaacc
      361 gatttgggaa cagatgctaa tagaacaacc tatttactag atggtaaaaa attcaaattt
      421 agcgttaaac gtggggagac tgctaaagta gttcttgatg ttaccaacta caaacgacct
      481 gtaattacta aggtaattga taaagaaacc gttgatactg gcgaagatgt tcaatataca
      541 ttgaaaccat caattcctgg taatattggt aattacgaaa aatatgttgt caaagatgtt
      601 ctaggtgcag agttaactta tgattcatca gaagtgatcg aaggaatgac tttagatact
      661 gattacagta tcgaacgtga cggacaaacg acaaactatg tctttactga atcaggtatg
      721 gataagcttc agaaacaatt tgatgcaggg gatcgcttct ttacaatttt gatgaacgct
      781 catactaata gtaaagctaa aatggataca ccacttaaga atgatgtttc aacgatctac
      841 aacaatggat atgaacccga agacgaaaca ccagttgatc acgcttatac aatcattggt
      901 gatcaccaat tcattaaaac agacggagat acaagcaaag cattgagtgg agcaaaattt
      961 gtagtgaaga acaaaattga tccgaataaa gttacttacc taaaacaaga tccaacaacg
     1021 aaagctgtta cttggattgc agataaatct aaagctactg aattcgtttc cggatcagac
     1081 ggtaagttcc aaattgaagg tttgcaatat acctatgatg tagattttga taaacaaggt
     1141 aatccaacaa tcacagataa aaaaatcaat gattatgctc ttgaagaagt tgttgctcct
     1201 tcaggatata caccaactag aaacgatgtt ccatttgtgg tatcgaatga tgatgcttct
     1261 agcgaagtaa cagacatcaa gaaccttaaa acacctgaaa cagttcgtac aggtggaatt
     1321 ggaacagtag tatttctagt ggttgcaggt gtggcaggaa cagggctata tgttgtctac
     1381 aaacgagaac aacacaaagc ttaatgaaat aaaattccct agagtgtttt tggctctagg
     1441 gtttcttttt aaaaagtgtg tgaaaagtag gtatgagcat gaaaaagaag aatatcaagt
     1501 tgatcatctt aaaaattggg atgttgctat gtttaggagt aggtctatat cctgtaatta
     1561 ctcaggctta tacctactac caaactcaac aaacactaaa ctcctataag gaaaaatatg
     1621 atgaagcttc tgaaaaggag aatgaagaag tcttcaagag atattcagaa gttaatagga
     1681 aacgaaaaga cacagaacaa acctcagtca cgattcctga agataataac aatattccgt
     1741 caaatggaaa tcttcagaag gataaattag gtgaattagt gggtgaaatt cttataccaa
     1801 agattaatgt ggatttgcca atttatgatg gtgtaaacga tttccaacta tcagcaggag
     1861 caggaatatt aaccggaact gattatccaa ctggtgggat tggaaaccat tctgttatat
     1921 cagctcattc aggcattcct aataaaaaat attttacaga tttagagttg ctcaaacttg
     1981 gtgataaatt ttttattgat atttcaggga aaaaacttgc ttatcaagta gatcggataa
     2041 aaacaattct accaaccgaa tttgaggatt tgcttcctgt ggcaggaaaa gattatgtaa
     2101 cacttatgac atgtacacct gttggagata attctcatag gttactcgtt agaggggtta
     2161 gcatacctta ccaaaaaagt attgttgaga aagaaaagaa gaaagaagat ttaaaggtaa
     2221 aggcgcagat gaactggtat cctatcgtag ttgtaggggt tttattaata atagctgtga
     2281 tagtcattat taaaaaaata gtagcaaaga gacgaggaat gaaaaatgaa gaataaaata
     2341 ctactttttt tgactcttat ttgcactttg gttttaacaa tttatcctgc aaatcagctc
     2401 tatcatcaat tcaaaacaaa agagaatgtg gcggaaatcc aagagaaaat tgagaaaaaa
     2461 caaactcctt caaaagagga agtggcaaaa aaagtcgaac aaataaaatc gaacagcggt
     2521 aaccaaggat tagacaatac tcagaaaaca gataaaggag tagacaatac tcaggaaaca
     2581 gacggtggat catttgatat tcgtgaagct gaaggaaagg tcaatccata cacaggagat
     2641 gttttttcaa aaaatcaaac gaaagatttt gaaccatcta cctattcagg agcggttggt
     2701 tatgtctata ttccaaaaat aaatgtgcta gagcttttgt ttctaggcgc ttcacaggag
     2761 catttatata gaggtgtggc acaaatgagt gctactagtc tacctttatc aggtaaaggt
     2821 catcaaacag taatagcagg tcatcgtggc ggttatacgt caccacaatt tctttatgtt
     2881 gatcaattag tagccggaga ccacatttat atagattatc ttggtgaaaa agcagtttat
     2941 acggttttcc gaacagacgt tattagaaat tacggtcaga attcaacact gactgatatt
     3001 cctgatttag atatgttaac actttatact tgtcatccat atcctcagaa ctacgatcgt
     3061 ttattagtta gaagtctaag agatgaaaaa gcaaaataca tcacgccaca agtaacagtt
     3121 ggcaataccg catacgctga tgccattgat agtgaatccg tttcagataa gccagaaact
     3181 gttacatctt ctaatgattt atttgaggat gttgatcgtg ccaacaagga attagatgga
     3241 aataatgatc ttaatattcc ggaacaaggt agcgtagaga tgcaagacag aacgtttgct
     3301 gaaaaggcaa gttcaatatt tacaggaatt accaataaac atcagaaaat gcttttagtc
     3361 atttggactg tattaggagc attagcaagt ctaaaggttc ttattctttt aatcaaagag
     3421 tttagaaaag cataatgaag attataaaaa ttgatagagt acagtaaaaa gggattgata
     3481 ctattattga tctctttttt attttgaata gagctagatt cacttttaaa taaaataaaa
     3541 actgatctta attccaaaga aaaagtcaag aaaaaaatac aacaaatttt tttatcagaa
     3601 tatttatcag aatattatta gaaaagataa actcaaaagc ttaatttaat agggttattg
     3661 gttgtattta gaaataatgt atggaaaagc tataaagcaa tagataagat gtagaaaatc
     3721 ttatttgtga atgaaaaaca agtcatcgta aaatattaat aggaggtgta gtaatggtgg
     3781 aaatatcaaa aacagcttta gactcattag ctaaactatt tagtagcagg tttgatggtg
     3841 cagaatttgt tgaatatcta agccaaaacg aaagtggaaa aactctgatc gaaacactga
     3901 aaaaagacta tttgaatgaa aatgactttc cgacatggac gaatattgaa gatgctacat
     3961 gggatgtagt gtgtacgata tattctcctt gggtaacgat tgctgatttt gttttagata
     4021 aaattggagg ggatacatac cctgatgcca aatctcttgc tacaagtgac tttaaaacgt
     4081 tctatggtta tctaatgggt ctaataggca atggaataga tttgaaagtt acgccaaaac
     4141 aaacacctga agaaattgaa aaagaaaaag gagaggttca aatggcttat gcagatgaca
     4201 gagaaaaagt aaaattattg gaaggtaaat tatctgaaga agaacttgga aaaatggtgc
     4261 aagaaatcaa tgaagacagc aactattttc ctgaattgaa ggtatatcaa aacgacgaag
     4321 atttctttaa tgaaatgttt agtaatagtc catgggaagc agtgagtaaa acaaatttag
     4381 gagaatggaa ctttcaagat gatctagttg catttagcaa tgatgggtat ttacgatcat
     4441 attcagaaag tgagtataga gctgaattaa aaacgtatga aacggatatt ctgatgagct
     4501 atttaaatct attagagagt ggtcagggag agttaagaga taaagttgaa gaactattaa
     4561 aagaagagct atagggatta tttcaacata aacacaagca gaaaactacc aaacaaaacg
     4621 taccatgcaa aaagccggtt aaaccggctt tttgctctat tatgagagga gatcaaacca
     4681 tgagtgcaac aaccgatatg gctttagaag cagtaataaa agataataca ggaaatgtcg
     4741 aaaatgtaga agactttagc gatattgcaa tcaatctttc agcatggatt tcagctgtag
     4801 gagaagaaaa tatcgaattt gagatcaaga atatggatga cttaaaagca aaatttcctt
     4861 ttatcgcatc gaaactaagt caatttgccg aggagaaatc gtggaatgaa ttagttagtt
     4921 tttccgaaaa aatttatgat attacgcaag aagatgagtg tgtttcatgg gaaatgaata
     4981 atcaatacaa gaaaattgat gctttcgtca aatcacgtat agaaggaaca gcttttgagt
     5041 ttgaaggtat agataccgca gataattggt taaataaaga atcctttatt aaaagtatag
     5101 cggatgatct tgaaaaacaa tggtctaagg aagactatgc agagtaccta aaaacagaaa
     5161 gagatacaga ggtgaaagaa aaactcactg aggtatgttt ggatgaacat attgatccca
     5221 atatcgtaaa acaagaagac tatgaagtgg ttattgataa agttcctact gattttgagg
     5281 aagttgtaaa gtatatagtt gaagaagcat tatcgcaata ctcttcagat gaggaagtgg
     5341 acgaagaaac tgttcgtacc agagtttggc aactggtttt tgatgacaaa gattataaat
     5401 atttgagtga tcaaaactcg gttaattgga gtgtacgatt attaattgat caaaatgata
     5461 ttgaataaaa tagagctgaa aagctctatt ttttttgata aaaaaactag ctattttttt
     5521 aaaaataatt tggttcaaaa tttatttttt taacttgata attttttgcc atcctttttt
     5581 aaattggcta tatatcaagg tttatagtgg ggataaataa aaatacattt gacaaaattt
     5641 acaatatagt ttattatata tttatcttat tatgataaca caataaaagg aatgatggta
     5701 tgaaaaagaa gaagtttagt cgatcgttgt taggaatttt agttttagtt gcggttggtc
     5761 ttagtggcaa gggttacttt cagaataaca gcactcagga acatgatttt cagatcaaaa
     5821 gtagtgttca ggtaggtaat aaagcagaca gtgaatcaaa agaattttta gggatacgtt
     5881 atgatccaaa caaatatcca aataatatcg taaacattaa tgatgggaaa tatacactga
     5941 caaaagaaga ccatgaagca ttatcagaaa aaggcagtca aaccttttgg gtaaattatg
     6001 cagatttaga tcaacttgga agagcaggag atgttgaagc gttgatcacc tatgatgcta
     6061 taaattctca ctcatctaaa aatgtggaac gtccatcttt caaaagttct gtccatattg
     6121 caggagaata caaggatgga gtatttgatc aagagagaga tacttgggtc ggcaaagatt
     6181 caaacaatga aatcactcag ctagaaggat ataaaggtta tctctataac aaaagtcatt
     6241 cattagcatg gtcgctagga ggagatatgg aggtacacaa tttaacactt ggaacgagag
     6301 cgcaaaatgt tgggaaaaat gatcagaatg gagggatggc ttactcagaa acgattgtac
     6361 gtgatgcaat ctataaaaat aagaatgaaa aagtattgta taaagttgat ccaatttata
     6421 aagggaatga attggttcct cgtggttctc atgtcgaagc tttctcagtt aacgataatg
     6481 gaaaaacggt aaaccttaat gtttatgtat ttaacaatca aaaaggaatc aacatcaact
     6541 atcaaaatgg tacttgggta agagattaga aattgagaag gtgaagaatt ggaaacaata
     6601 agcatcaatg gattagataa atcagttgca aatcgaataa aaaaaggaaa tggctactat
     6661 cttctgaaga agggtaaata ttcagttgct agagagttag aaacaatgag tatcgtgtat
     6721 gtagctaaga atcattttga cgtagaacag gctatttttg atccagattc tgaaataggg
     6781 aaatgtgttg aattatcaga gaaaactgtt agtacaaaag gggcgatgat cattgagaga
     6841 gaaatgttag acgaaatcag agtaatcatt cctaaagata cgcagatgtg tgaggacagc
     6901 ataagtgacg atgaaccaaa atcagaaaga aaataaggag atattcagat gagcgaaaaa
     6961 tccattgaat taaaggaata taagcgaagc gatggagaaa aatattatcc tgtagccagt
     7021 ttttctaaat cagtatatct ggttggacgt gcattggtag ttggtgcaat tggcatatat
     7081 attttgcatt gggtaacaaa ggttgtacaa ctgcttgtag ataccttagt aagtattctt
     7141 gtctcgttaa atgatgttaa cggaacagat gatttaggat taaaagaaat gtttaaagag
     7201 ttactcaatg cgttttttcc caattattta agttattcat ataatgtaca aagctttttt
     7261 gcaaagctac acttggtaga catctctatt ttagtattag aagcaggaac ggtattcttt
     7321 gttggatatt tggttacttt gatgttcagg catcactatg gagaacaaag accatttttt
     7381 gatgatatgg aaagtagaag gttgaaaaga gaagctctag aagctatgga tgtgaagtat
     7441 gatccaaaca agattatagg agatgaaacg aacaacaaaa aaagaaaacg aaacaaaacg
     7501 caattagaat ccgatgtgct atcaacgctt aaaaatatca aaatttctgt tcatacaaga
     7561 cgtgatctaa gtgtcaatga agatattcga gaatatatcg taaaaatccc gcaaccaaaa
     7621 gggaagaaag ttagagaaac agttttacga caaattcaag atttagggtc gatcctgaca
     7681 agagcaacaa gcggacgagc caaatttggt gaaaaagaag atgaagtttc aggcgattat
     7741 ttcattttcc gagcatcagt aaatattgaa agagaacgta aggaaagagt aagtaccaaa
     7801 aacaaagaaa cggaaagagc aacatcttca atcgcagttt caaattcaca attttctttt
     7861 ccaatcaatc tctttgccga taattccaaa aatatccaaa accaaaccga aaaagctaga
     7921 aaatatgccg aaactcaaaa gcaatcttta gaactttatc tatcttctgc tgacgtacct
     7981 gcaagtctaa cagatatgca cgttgggaat acatctattg aatatttgtt tactttatct
     8041 cgtaccagaa aaattcaact aagctcaatg aaagaagaaa ttgaaactta cttgcatata
     8101 tcaggtgtaa tcgtgcggat caacgcaggg cgtattcaaa ttactctacc attaggagaa
     8161 aatgatcgta ttccaattga tgtaccatca atgatcaata gcgtgtttta aaagacatca
     8221 caaatagaaa cagaaaatac aaactaccag ttcacaaaca gaaaagtacc gagaaaagtg
     8281 aggaatagaa atggctatga aattattaga gaaagtcaaa aagagagccg aaaaagctga
     8341 atctattttg ctagataaag aacctaatat aaagcttaat ggaaaattca gtgtcaatat
     8401 gttgaacaca aaggctaaag acccgacaga tgctattttt ggaattaagc ctgatggcac
     8461 aattgctcat ttttgcatca gcgaaatgcc acacatgttg gttgcaggaa cgacaggatc
     8521 agggaaatca gtattactga atgaaattct tgttacagca atgtgtcact caacgcctga
     8581 tgaattaaaa ataggaattg tagacccaaa aagggttgaa tttggaagat acaaaaaatt
     8641 gccgttcatg ttggctgatc caattacaga tatggatgaa gcttatgatt tttttgaata
     8701 tcttgtgatc ttgatgcatg aacgttacaa actgatggaa aaagcagggt tacaaaacat
     8761 cacgctatat aacgaatatg ccgaaaaaaa tggattagat agatttccct atgtgatctt
     8821 attagttgat gaatattcac agttggttgg aacacataaa gaagtagaag gtttgattgt
     8881 tcaattagga caaatggcaa gggcagcagg tattcatgtt atcttagcta cacaatctcc
     8941 tcgatcaaca gttgtcactg gtattatcaa agccaatttt ccgtcacgtg tttgccttat
     9001 ggtagcaagt gatttagaaa gtaggatcgt attagatgaa ggcggtggag aaagcctaag
     9061 tccaaaggga gatatgatta ttaagctcgt taatggaagt atggttagag cgcagggagc
     9121 ttacatcagt aacaaagaaa ttgaaacaat tttcaatcac cttagaaata ctatgccaga
     9181 accagaatat gttgattacc gagcaatcgt agctgaatct cgtggtgagg gtggagaaga
     9241 cgaggatttt ggtggaattg gtaaagctcc tgtaagaaaa tctgctaaaa gaaatttgaa
     9301 cacaccttta gcaaattcat catctaaaga gccatcaatt gaaaagaaga gtacgtttgc
     9361 tctaaaactt gaaaaaaaca aagaagaaaa agaaaaacaa ggagctaaaa cactagaaaa
     9421 acttcaagcc gaaaaattag aaaaagtttt aaaagcatta gataaagctg attctgaacc
     9481 gcaaacctca aacaacacga ataatgaaca gccgatttca tctaaaacta aaccgaaaac
     9541 tactgatccg aaagtttcag taaacgtgac catcacaacc gataagtctg agaagaaaca
     9601 agtaaacgga acgacttcac ctatcatgca aaaaatgttg aaagccaaaa aacaggtgca
     9661 taaatgatca ttgtcagttc ttttttgagg tggcttacgc catataaata tacagatgaa
     9721 gtagttgtga aacgaacgaa gaaaagagat aaaaaagcgg tactaactcc taaacagatg
     9781 cttgatcatg gcattttagg ggtgaaggtt ttagaagtga ataaaccgtt tgaatcaaat
     9841 agtgcaagag aaaagttgct tggagctgaa gccgatgtcg catattttta ccatgacaaa
     9901 agcaagcatc aaatagctat taaattcaat ccaaaatcgg catggcggta tcaagcaaga
     9961 gatgacttga aacgtaggaa acttagacca atgctctatc cagaaggaaa agaagcattt
    10021 gatagaaccc acatcgttcc cattggtttt catggtacag aatcagataa ccgcctatta
    10081 gtaggttgga acagcaatca aaatagagtt cagatcaata aatttgagaa gaaagtaagg
    10141 aaattcaacg atcgaaaaac gatcctatgg tttgcagata tttgtaaaca gccggatgga
    10201 acagcaaagt gggaagcggt gatatttgac gaaaatggta aaaaagtaat gtctgaaaca
    10261 tggcatgata aagctaaatt tgtttggcag tagcagtaga ggtgaaaaag attgctagaa
    10321 ctttttgaag caaatagaac aggctttaat caaaagttag aagtgggaga aactatagaa
    10381 tataaagata aaaactatat tattatcgga atctataaca cgagaattaa cagaataggt
    10441 gatccacgta taaccagtga cttggtttgt caatctgtta actattcacc tgatctgaca
    10501 agcaggtata agaaatacgt gttattagaa tatagacaca agattactga tgaaattggt
    10561 gtaaacaaca cacgaaacat atttaagatc ggaacagtga ttccttatag taacaacgaa
    10621 gaaaaaatat tttatcagat ttacggaata gaaaagtttg aatatgaaca tgtcgatctg
    10681 ctagtgacgt accaaatgcg tttaatagaa ccttggtcac aggcagaaat tgataaagca
    10741 gtaaaattga accgattatc aaaatttaac gtattaggaa atgcctttta aaaatcgcaa
    10801 tacacaaaca gaaaaatacc aaaaaattag caatgaacgt aggtaagaac atgacaataa
    10861 ataataataa ccctaaaaaa tataatgctc ttgtccaatg ctatatcgaa aacaaaatta
    10921 caatgcaaga agtcgctgat gaattaggca aatcaaagag cctagtatca cacgatttta
    10981 agcacaatgt ttttccgaac ccgattgagc tgaggaaaaa atttcaaaag agagtttatc
    11041 aagaaatagg taagtggatt gatgaaggga atgcaattga ccctttaatt gaaaaatatc
    11101 cttttttaaa aagcaaaaat aaaattcaaa ggttaattcg tcaaggcgat attcaagcta
    11161 aagaacctgt tttgacaacc aatgatttgg ctaaaataaa tcttcataaa caaattattg
    11221 aagactttga aaaaaatctt tcaacaactg aaattttaaa aaaatacaat attccgagag
    11281 gaaagctaac atcgactctt tttagagaat atggagaatc ggcagttact aaagcaaagg
    11341 aagtagcgaa tgagagaaaa aaacaaaaat agtagtcgat acctagtagc gactgtagtt
    11401 tttgtttgtg tattgatttt cctgtttgat tttttgcaga ttggaaaaag cataattgga
    11461 aatacattga gtgcaaagga aatggttgct ttaggtggtt tgtatgaagg acacttgttc
    11521 ccgaatatat tcatttctat cgtttctcat attaacttgt accatttaat ttctaattta
    11581 ttgttcttag tagttcttga agaagcactg aatgatcagt atgagtggtt aacattattg
    11641 atcgttttaa tcatatcagg atttctagga agcttagttc cttttctgct acacagcaac
    11701 atcgtaagta tggggcttag tggaggtatt tttggatgga ttgctttatt tgcgattgca
    11761 tacatccgtt ttgatagcaa gaaacattta ttactaagtg tttttgtatt attgataatc
    11821 ggcattttac tggtatctac ttttgaatca ggcgagacaa acgtcttgtc acatgtaatc
    11881 ggatttgtct gtgggatgat cttgggattt attctaaaga acgtttcatt gatcaaacaa
    11941 aagaattaaa agggagtgct aaaaatgcca aaaaaaaaag aaaagaaaat tccagtgttt
    12001 ttgtctgtct cagaaatgtt atcacttcaa atactgttaa gaaaaaagat tgaaacaata
    12061 gacgaagaag ttagaaataa aagggctatg tacgaacaac gtgaagcgct aagagaacga
    12121 atccactatt ttgatgatat acacactaaa atctatgaag cacgtacaaa tcacgaagac
    12181 gaaagcaaat taggattagc tcaacaacga atatttgaat ggctgaatac gaatactgag
    12241 caagaaccgt taagagcaat tagtaatctt tttgaatttg gaacgaagca ttggtttaac
    12301 gctgtagaaa aggatgctga cttacaagaa gcgttcgata cactaagcga ggatagcaaa
    12361 tccatgcgag acttattgat cacttatcta aatgagcatg aatttgaaca gacgaaagtt
    12421 aaaaagaaaa ataaaaaata gaaagttgtg atgtatcatg ctgtttacct tattaggtta
    12481 tttatgttta gttcttgctt tttttggagc aattgctata tttgtcggtc agtttcaaga
    12541 aaataatccg ataatgcgta acataggcat tggaattgtt atcatatcct tgattgtatt
    12601 tggtgccaca aagatttttg ggagtaatga tcagcagttt ggtatttctg gagttgataa
    12661 tagttatgct ccagattcat tgggaagagt agtgatagat ggaacgtttg agaatgtaac
    12721 gagctacaaa aatgtttcat tgacagtgaa agaagatgac aagaaagcat cagatgacgt
    12781 tctcgctacg atcaagccgt ctcaatcaca gtttgaagtg ttttatacag tcaaagagaa
    12841 taaccctgta cgcaagctaa cattagtttt taacgttgac ggagaagaat actataaaga
    12901 tgtaaaaatc aataaagcat caacgaaaaa gattgtcgaa tcagcgaaaa aagaagtagc
    12961 tacagaagaa agtacaactg aatcaagttc tactgtcgaa gttgaactac cagataaaat
    13021 tgattttgat cctgaaaaag tcaaatcgtt tgaatctgtt tctggttcta tggcagatcg
    13081 gtttaacact gaagttttca aagctatcaa aactgttaaa ttggatactg aaaagcaaag
    13141 gattaatgtt accttattca atgcagttga atctatggat caggataaaa aagttacggt
    13201 tgcaagagaa ctagggaaaa gagtaattgg attgtatcga ggtacggcaa aaccagattc
    13261 agacggttca gatttgccag ttcacgtagt ttatgaagac ggtaaagttt ttggagactc
    13321 tacagcaaat gaagcactaa cgattaaatt agctaaatag ccggaaaatt ataataaaca
    13381 agtggtgtca caccaccatc gcataggtgt gacaccttgg agatgattac tgatggtagt
    13441 aaataaagaa aaagtaagaa agtatgttac tttaaaaata gcttctgttg aatatttaga
    13501 aaagcgagct aaagaagaag aaaaaacaag aggtaagaag gtaacagttt cagagttgtt
    13561 agaagaatta attgagagtg accaaacaat aagacgtgca tttaccaata ggtctgttaa
    13621 aagttagggt gatactatct ttaatttcaa ccaaatttct aatatatgat gaatagtttc
    13681 cttgtacaaa atatctaatt aagagataaa agttaggagg attcattcaa aatgattatc
    13741 caaaagtttg actggaaaat gaaattagga attagtcttg taatattggg atttattatt
    13801 tttttgggga taaaagcaca aaaaaatcaa tctacttttg aaactatgtc ttctcaaatt
    13861 attgaggaat gttccaaaat ttattattgt gagaacaaaa ctgaagaatc taatggcttt
    13921 tattatatcg atcaaccaca tgatgttgtt ataaaaatgg agtcacatga agtaaacgaa
    13981 tggattcaaa gaagtactaa tgtatcaaaa aagttagaaa gatattctca aacgttttta
    14041 agtaaagtca ttttttcaaa ttgcaaaaat gaagaaacgt ctcaaagagt agctagttct
    14101 ttagaaaatt taaaattttc tgtttcaaaa aaagagaaat ttgtagattt acgaaaataa
    14161 actatcgaaa gtagtatgat tatgatctta aaatatcatc ttaagagaag aattacgatt
    14221 gtttatagga tttacaaaat gaataagttt gaagagtgat ttggtcgcag actagatcac
    14281 tctttttgtg taaaaaaact ttacacttat ttttgagtgt gttaagattt ttttatatag
    14341 agtgacggtt tcttgtcaga gtggatgata accatcaata ttggaggaat aataagtgga
    14401 aaacaataat gatattagat attttagcat tggggaaaga gttacaacag taagtgaatt
    14461 atcaaaggat tatagagata aagaaggaac gattatttct cttaaaaaga atcgaagagg
    14521 tgtttggcaa tatgttgttg atttagggga tggcgttaca gttgagtgta aagatagtca
    14581 actgatcaga aatgatggca gtcattccat tgaaaaagca attcttgtat tcccaaaagt
    14641 atcaagcaag attatgaatg actggttgct tggaaaattt ggtgagtaca ataaaaaaga
    14701 aatcgcagaa gtgatcggca aatcttatcc aacagtatta gggtatctaa aaaagataac
    14761 gaacaaagaa gcgaaagttg aggatgtttt aacagtaaga gaacttcagt cattaatcga
    14821 atatcggaat aaacaaataa caaatcctga gtaccttaaa aaagaagatg gtctattgaa
    14881 acaattggaa caggtaaata aaatccggaa aatgcaaaag gtagctcctt taagcctaga
    14941 taactttaaa aaaatctatt tgaaagagat cgaaaaagac taatgtggag tgtcacttta
    15001 ccgtttcaac ctgtatctgc accaagacca agtgcaagaa ggaatgttgc tgatgcaact
    15061 aatgacgtag cagagaaaag gatttcaacc tattatgata aaaagtatct cgattatttg
    15121 aaaaaaataa aaagctttat cgaagatcaa ggtttgttag atgatgaatt ttactcgatc
    15181 gtaaatgatc cgatgggtgc aattatggaa gtggactttt actatcagat accaaaaagc
    15241 tataaaaaag tctataacat tatgaaaacg acagcgccag atttagataa tttagtaaaa
    15301 agtgctatgg atggtatttt caacatctca gctatacgag atagccatat cacagggtta
    15361 attgctttta aatacaatgt ggcaaacgaa ggaaaaacag ttgtgaggat caagcgatat
    15421 tcagaatttg agtgggatac ttctgaagga ttgaacggag atatttggga agccacattt
    15481 gatttcaaac cagtagcaac gccaagacca aagtctaaat ttagaggaaa tgggattatc
    15541 acttactatc caaaagagta taacgactat ttggaaaata tcaagaatac gcttaaagag
    15601 aaagatttgg taaatgatca actgaaaaag gtaatgaatg ctcctatggg ggttatagct
    15661 cagatagatt atttttacca agtgagaaaa gataagaaaa agcttgattc actgatgcgt
    15721 acttcacagc cggatattga taacttagtc aaaggaacat tggacggaat ttttaacaag
    15781 gaaattggca tcaaagatag cagggtggta ggtttaatag cttttaaata caatactttt
    15841 gaaaagtcga aaacaaacgt gcgactacag gagatgggct gatgaataat aacgaaaaaa
    15901 ttcttcatgt acttgatagc tttgaaacaa ttcaagagga actaaagaaa tatcgagatg
    15961 ttttggaaca aagatatgat tttgtcaatc agcaaaagag taatcacatg gatttcattc
    16021 tcaatatgaa tgacttgaag aagaaattag ttgaacgaaa agaacaagaa aagttgatca
    16081 aagcttactt tgaactcggt gaaaaagaag ttaaaaaggc aatggaactg aatgaagatc
    16141 gcagggtttt agatcagctg ttagaacaat tattggtgat gtttcaaaaa ggacgaatag
    16201 atgaggattt aattgaagaa gggttgagaa agtatcctgc aaactcaggt atcggaatag
    16261 tgctgaaagc gattgatgaa gaagaaattg aagatttcat accggcagag gattttgaat
    16321 cggcaatgga atatataaaa tattacagcc aagggatcac tgcttttaga gagtttgatc
    16381 ctgaagatgt tattcatgac ttgaacaacc taaaagaatg gtgtgaaggt tatgaagttg
    16441 atgattccgg tttggactac ttgatcagta ttatggaaat cgaagaagag atgccggata
    16501 agcctgatcc aacagatatt ttagaattaa tccatgatgc tagaaatcca attgcataca
    16561 tttctagagg atatactgta ttggaatact ataagcctta tatatcagcg atgaaccatt
    16621 taagacgtgt attaagggaa aaacgtgaat accgctcagt gttaaatgca tcgaatagat
    16681 tagaaaaagc tgtttcagag ctagatgctt attatcgtga acattacctg caagcaggtg
    16741 gtatgccaag aaatacgaaa gcaaatatca gtaggtatat aaaagaagct gagtaggcgg
    16801 agttagccca taaaatggca aaaggtaacc tgaaagaggc gttttgaatg aaaagggatg
    16861 tttttgaatt gtacgtatta gaacgtctag aagacggcat gatggcacaa gagataattg
    16921 atgaaattga tgatatgatc agcagatttg gtcaattgga attgaatgat ggaaacaaaa
    16981 attcggtagt gctacaacaa ttttggttaa ataatttttc gtataaggat tttatcaaag
    17041 aaaaggcaaa tagattgtac tgactaaatt cacgaggatc agtgatgaga gcttattgct
    17101 aagagcagtt aggctctttt ttgtttgtgt taaattattt tattttacaa tacacattta
    17161 tacacaaaaa atgttgacgg atagtacaca aatgtggtag gatatatttg taagcaagtt
    17221 agattaacaa gttaaaggaa ggaagagcag ttatgaaaaa agaagaacta atagatatgt
    17281 ttcaaattgt tgaacgagca aataatatgg gaattatgtt ttttgatcgt atttctttaa
    17341 agatggattt aagcgttgcc caccaagaat ttaatctacg attaaaagct ttattgattt
    17401 ctgatgatgt taatttcgct catgatgttg taggtataca gaaccatata gatagagaaa
    17461 acaaacgtat gggtgatgga ttcttaccaa gatattcaag tttatgattt ggcgaaagat
    17521 tcaagagtat gtgtcattat tgataggtac tcttgaattg gaaaggagga atgaatttaa
    17581 gacaacaacg attatgatct taaaacaatc gtttatgtaa ttggcaatct tacgaataat
    17641 tttcatgcaa taatgacaat aaaaaatgaa aaggggtaaa atcaaatgaa aaaattttta
    17701 gtaggaattc tattcacaat ggggctagtg ctattatcac cttcagtttc agcagaaagc
    17761 tctatttcca tgtatcgtat gtataatccc aatagcggag agcatctata cactagaagc
    17821 gttggagaaa gagacaatct gaaacgttat ggatggcatt acgaaggtgt cgcgtggcaa
    17881 gctccaacaa gtggttcccc agtttatcgc ttgtacaaca gatataacgg tgaacatttt
    17941 tacaccgttg atgccggaga aagagataag atttcgcaca caggatggac gtacgaaggt
    18001 gttgcgttct attcctacaa cggagcaaat agagttcctg tgacacgctt gtacaacaaa
    18061 tacgtgaatt ggcaccatta cacgttagat caaaatgaaa agaacgtaat tagtaaacaa
    18121 ggttggaaga ttgaaggtac gggttggtat gcaatgccaa acagcgcacc aaatcctaca
    18181 cctgcacctg ctccaagtaa tgaaaaagtg ttaaatgttc ctgtcatttg gcaaggtaat
    18241 acgatgttgt gtgaaggtgc ttccctgtta gagggattgc actacaaagg agtagcgaca
    18301 aatcaaaact taaccagttt tgtaaactat atgccaagag ctaatgacaa taatccatat
    18361 catggctatt ctggcgaatg gcgctacaac gtaaatggta cgtatcaagg catgatgcct
    18421 gatccagtag ttaaatgggc aaaaaatgtt ggtggtaatg ccgttaatat cactggttca
    18481 ggaacgaatg ggatcaagaa tgaaattcgc aatggtaatc cggttgtaac ttggattact
    18541 tataactatg cttcacctca attcaaacaa atgccttggg gacgtgcagt ctggaatgga
    18601 catgtggttc tcgtggatgg cttcaaaaat ggtgcatacc atgttgttga ccctgtattt
    18661 ggcgcaaaat ggatcaatgc aagcacgtat gagagagcat ttaatgtgac tggaatggcg
    18721 gttgctgtac gttaattgat agaattaata aataaatgaa ataagagctt aattactgaa
    18781 atggtaaatg ggctcttatt ttttagaaaa aaactgtttt ttgtaatacg aatttataac
    18841 aaaaaacgtt gaatgtagta cacaaaagtg gtatattaaa accgtagaga ttatgcgaag
    18901 aggtgatttt gatgaaccaa agaagaagaa aaaggctaga agaaataagc gagcgactat
    18961 ccgagttaaa ggatgagtta gaagtaattc aagaagaaga ggaagagtac aaagaaaata
    19021 tacctgaaaa tctacaggga tcagaaagat atgaaaaagc agaagaatcc gttgatcaac
    19081 ttgaagaagc tgttgatggt ttatccgaca tattagaatt gattgaagat atagctagct
    19141 agagatgatt aatgaagatt tcaggagtga gcaaaaaatg attgatattt cactgataga
    19201 gaagctactt ttttcaaata agtacacagc caaagagttg gctacaagat ggcaaatatc
    19261 cgaatcagct attaaagcct atcgtcaaaa taagggaagc gtaggatggc gtgattggaa
    19321 aggaatcagc attggaaaag cagaagacat aatgaaacgt gaaggatttc aagctgacaa
    19381 tgattctaaa aaagttgaat agtttaatag tcgccaagaa aagtaccagg acgaggggaa
    19441 aggtagatta caaaggaaaa cacttttaaa taaacttcgc aaaagcatca ggttatgagt
    19501 ggctagggct atacgaatgt gatgaaaaca aagggcttta atgtcaaaga aaacgcaata
    19561 gggggggaac aaaattttta aaaaagatat ttatgtttgg tttaatggaa aaaaatatat
    19621 gactactaac aaaaggtggt cagagctagt acaaaataca gttaaaaaag tcatgccaga
    19681 tggatatcat ttcaactata tacaatgctc acaaacaact gaaagtattt atttttcaat
    19741 attaaatgag aatagactct attatttcag ggtaagttca caccatagga aaaagagaga
    19801 agataatgtt acgacatttt tatcgggaaa atacaatggc ttcaaagagt taagtgaagc
    19861 cattggtgag tatctaaaaa gtaatagatt tgtcagaatt tctgacaaac atatattgat
    19921 cttgttactt tatttgaata atagcttcaa attgttttgg agagacgaca ctttctacta
    19981 tattgagaac ggcatggata tttattctgg tatcaacggc atattaaaac attatattaa
    20041 ggcgttactt cgatatgggg ttctttatca agaagattta agtaaccaaa ttaagattag
    20101 taattctgga aagtcattac tggcaaaata taaaaaattg ctttctccta tctacaaagg
    20161 aatagatatg gaagctgtaa attctgaaac tttatttaat ttagttgttg atgaaaagtt
    20221 tgcgaatgaa ttttttaaaa gtaaaaaaat tgaaagacta aataacctgt ttcctgaaaa
    20281 tgcaataaaa gcttatgtag ataataataa atcacgatta tacaactggt ttattcccaa
    20341 acggttgcga ggaaaaatca aaattggcga tcaagtgaaa gtaattaatc taaatcatac
    20401 taagatcgtt accgtaacgg agttgtatat agctgaaaaa gaagatatta caaaaatgaa
    20461 accagttatt aaaatactag ataacaaaga gtgagtggaa tatatggtag caaaaaagaa
    20521 tactctaaaa gaagcttctg aaaaatatca agatatgaaa agacgaggat taataccaaa
    20581 ggacaaaaaa ggtatatgga gagaatttgt taaggaagtc agtagtgatc gaagtggcgg
    20641 tgactttgtt ggacgatata cagcaaaagg ttatagcagg cgcttaagga tcactgttga
    20701 atatggaacg aatgaatcag ctacagagct gaagcaaagg tacttagata cacgaagaga
    20761 agcttcaaaa aaatatgatg atttcaagag cagaggtcta attcctaaga agaaaaaggg
    20821 gatttggcgt gagtttttca aagaagaaga tagcaatcgg aataagggag acttcattgg
    20881 acgatacaca gcaaaaggct acactaagaa tttagggatc aaggttgaat acggaactgg
    20941 tgaaagttca tgggaattga gaaatcgcta tttaaataca ttgttagaga aatataaagc
    21001 agaattattg gagttgttcg atgttaatga ttttatcgag atggctgaaa agtatggtca
    21061 tgaagaaggt tttatgatca atgacattat ggttattgat aattttacgg atttattagc
    21121 acttgcgaaa aaagcaaggg atttaagaga aacgcagatg tatctattca gaaataaaaa
    21181 cggaagaaac cggagaactg aatcgagtga gattgaagat tactatgatg cctttacaca
    21241 cgatctggga gcctttgtag acgagataag tggtgtaatg aacaaatata gggtctagaa
    21301 taaaaaagcc ggagatgagc agataaatgg gaaacaatag catatacaca cctgaaatgc
    21361 agaataaaat taaaagactt gtcggattag ggtggatgac aagaggaaaa ttagattctt
    21421 ttagtacgat caaatatctt ttgatggaca tttaccgtca gaacaagcaa acaggaatac
    21481 attttttggg ctatgataag ccggaagatg cacctttctt tgttccaagt tcagcctata
    21541 gagccagtac ctccggtagc atgtataact tggatttgtt tgaggaagga gcaaagatga
    21601 tcgaatatga tttcaacgaa gcctattcga ggatcatgag aaattatcct ttgccgtcaa
    21661 acacttattt gaaagcaaaa tataatacag atcaagctat tttgagagcg ataaatagac
    21721 caaaagtaag accgtatgaa aatttaaaaa cgtatatgtt ctttttggtt aattttgaag
    21781 gaacagccaa agaaggaaca tatgctaatc caaaatcaca gtcgatgttt gcggattatg
    21841 gcgaaaatcc caaaacagct aatgagccta tttggttatc ggagattgag ctaacactta
    21901 tccttgatta ttatgatatt aaagagatgg aagtcctaga tagccacaca tttttgacga
    21961 aaaaaggatt actcaaagaa tattttgaaa gaatcgagcc attaaaaact gatcctgata
    22021 cgttgaagct ttataaaaat cttagaacga aagtatttgg tgctatcgga caaaaatcat
    22081 taagagaagg agaaacaagc accttcgata aatacagtat gtataatcga gcgtattcct
    22141 ccatggtagc aggcgtattt agagatcgta tggttagata tgaacagaaa tacgttcata
    22201 gcgcatatgg gctattaaaa attcgtactg atggattgta tttcagatcc gaagtaccag
    22261 agtttgaaga gctgtataaa cttggagtag ttaagaaaaa agttcataca atatccaaag
    22321 aagaagtcga aaaagcttta gaatttaaaa acaaaggatt tgaaaataag ggctcctttg
    22381 ggaaatctaa agatgaagtt aagggtgttg tgatagacga tgatttcgag tttgattttg
    22441 actaaatact ctttggaaaa gatggtagga ggtaaatatg aagaagaaaa ttgtggaagg
    22501 aaatgtaggt gttattattc taaaagattc aactgactat gctaaatcaa tggcactaaa
    22561 agacgaaaaa gttgcacgca tggataaaga tttagttgaa tttctgatgg atgatacaga
    22621 aagattaaaa ggggatcagt ttttgagttt aatgaaagac aaaggcttaa acacatatgg
    22681 tttatttgat tataagaaat tagctataga gtgggtaaaa gaaggagaaa tattttttat
    22741 tgaagaatgg gaaggtactg agaacttagt tcttcatcag tatcttttga aagcttaatc
    22801 taagtaaaac agttctgact agttatgctt gtcagaattt caaaggaagt tttccataac
    22861 catcatcatt caaaaataaa ggatttgaaa ataagggttc ctttgggaaa tctaaagatg
    22921 aagttaaggg tgttgtgata gacgatgatt tcaagtttga ttttgactag atttttttgc
    22981 aatgtttaag agtaaacaag gtaagaacta agatcatgaa gattgagagt aaagaagata
    23041 gaggacgaat gtatgagaac acactatatt tatttagaag ataaaattgt agatgaaatt
    23101 ccggcaaaaa gtcgagaaca agctagaaaa ttatttattg aaaaatacaa agagatcaat
    23161 ggagatagtg aagttccaaa agtggtcgta acagttagaa aaaggcaaac aaaaaagaga
    23221 agtacgtttc atgtcagccc tgatatatat aatcttatag agcgacatag aagtgaagat
    23281 aatatttcta tctctcaata tattgctaca gttattgatg aattgatttc aggaaagtat
    23341 gactatgctt ttgattttga gggtgaaaag gattacaaaa gtgttgctgt ctttcaatct
    23401 gattttgaga agctaaaaaa ctatgctggc aggagaaatg ttaattttgc aagactacta
    23461 gaagagttct actccgaaga gttgggagta ggaaatgaat acaagagaga aacttatgcg
    23521 aaattgtctc ataagttatc ccaagcgctt aaagaagtga cagaagttaa agcggaacta
    23581 gatgctaaac ttgaagaagt gaaaaaatat caaacagaga ggaagaacaa catgaagaat
    23641 caagtggaat ttgacgaaca gaaagaattt gaaaaagcaa gaaaacgcta taacaaaatt
    23701 atgaatgaac ttagctatga acacttaaca gttcatgaat aattgagtga gggtacagaa
    23761 aaatatacga tcgcagatat ggttaatgaa gtggaaactc ttttagcttc tttctttgaa
    23821 aatggtcacg taaacgatga atggcgaaaa gaagaaccta aaagggttaa cgcttatatt
    23881 gctcgattaa aaaggtttat tgataaatat aaggagaata gtgacaaatt ttctaaagat
    23941 aaataaggag agagtgtgat tacatttgcc agaaatcgaa ggaatatttt cgccaaaaat
    24001 aggtaaaaaa cagttagatt tcagcagttt tccgagcgta aatagagatt ataatctagg
    24061 tgatgaggta cagatactaa atattgattt caaagaacta gacttcgctg ttgctgtttc
    24121 caacaaaccc aaggcattgg aacgagtgaa aaaaatatac caagagaatg atcaaaatat
    24181 caaaaatatg atcaaaaagt atagacgaga taattcaaaa gttgaagacg ttttattcaa
    24241 cggtacggca aaacatgatt tgaatgttta tatggcatta ggagtggctt tgtactgtaa
    24301 ttcaattaat cagacagata ttttggttaa tatcgtgagg aatagctatc aaaaaatatt
    24361 aaaaataagt agcgaaacaa acccttatta tgcaatgaaa tatcttgggg ataatttatt
    24421 aaatgataaa aaattgatgc acaacactga agagctgtta tctaatttgg ctgcgattaa
    24481 ctttctattc aaaacaggtt ctgaagatta ttttatagat aacgtactaa aaggtgctta
    24541 cggaacagca ttttatccgc tttacaatat gaaacagcat aaagccgaac aaaaggataa
    24601 caaacttgaa attaaaaaga tgaaagaact attaggtata ggatttgtaa aacccaattc
    24661 aaaagtcact attgcttcgt tagcaactac taacaatgat gggaaaatag taaatgggtt
    24721 tgacgattca gatatagata tattgtcaaa atcatatttt tctttgtatg gagattttgc
    24781 agatttgatg ggtattgata gttacttagt caataatttt gagatcaagg gtagcgagct
    24841 gaatgatatt gtgtatttcg ttttaatctc tctatatgca attgatcaga ataccgagta
    24901 ccgacaaatt gactatttgc tagtttttta tagtttgtta tatcagttcg ttttaactcg
    24961 gttgtttaaa aatgctaagg aagaattgat cctgaattct gattatgaaa tagcaaaaca
    25021 aattgcaagt attagaaaag aaaacaaatc tgaagtaatc aaattaagaa aagaattaag
    25081 tattgcgagt aaagaaaacg gtcggttaga atctttagtc aaatcggcaa aaaaagaaga
    25141 ggaacttttg aagcaagaga ttaaagaatt aaaaggaatc agaaaagaac ttgaacagct
    25201 gaggtttgaa aataaagtat taaagcaatt gcaggagcaa gaacatagac aatcattaaa
    25261 tggtaaaact gaagagcctt cactgtctga aatgatagct tatctaaaac ataaaaacat
    25321 tcttttagtg ggaggacatg taaattttca aaataaatta actgaactat taccaaatat
    25381 taaaatgttt gatgtaagcg aaaataatgg aaagatactt aaaaatttga atacgtgtga
    25441 tgcgattttt ctatatacag attacttgaa tcatggtcta tattatcgtg ttatgagtga
    25501 aatttctaat aaagataaga tattttattt ggataatcat gtgaatatga acttaacaat
    25561 taaacgtatt tacgatcttt tatcacttat ataaagagag aagtgataaa taggacacaa
    25621 aatttggaaa gtaggtaagt cactatgaga gaactaactt tatcttctaa ggaattagaa
    25681 aagaaaactg ctttattaaa cgaaatattt agtgctgatt gtataatcca aagtttaaaa
    25741 gaaagttcat tttcaaaact aacagatcag gaaaaaattt cttatatgct aagtttagat
    25801 aatctaggaa ctaaatatga tgaatcagca agtagcgata agcttttgat tgaattagta
    25861 tcaagtctta gtagttttgg aaaaatgaaa agatttaatg tgatattaga aatttttgct
    25921 tggttaatcc cttcatttat ttggtttttt tatattttca tagttgaatc tgagttaata
    25981 gccaaggtct ctcttggact gttaattccg atataaatag gttcagcaat ttactttaat
    26041 aacttgtata cacgtattca aataaaaaag cagttattac atgaaggatt aagtggtaat
    26101 atgttaaaaa gaattgcatg ggataaagat aataaaacag atattgagct taaagaatat
    26161 aaatacaatt atttctcgag atgtatagat agaaagttac tgtttaaaaa agaaggatta
    26221 gaatttagtt ttgatgaaga tgaatttgaa aaattagtga tggactggcg taagaattta
    26281 ctggatacgg tacaattcta aaagcaattg aggtcgaaat tccgatactt gaatcatgat
    26341 ctatattatc gtgttatgag tgaaatttct aataaagata agatatttca tttggacagt
    26401 catgtgaata tgagcttaac aattaaacgt atttatgatc ttttatatgt ttataaacat
    26461 taatgaggtg ctaaaattga caacaataac tatgacaaaa aaaatagaag tatatgttga
    26521 tggatcacaa gaagaaaggg attccttttt tgattttttt tggaaaaata gtagagaata
    26581 tgtaaaagcg ttgaatctgg tatataaaca gctaatgttt gaagagttga taaaagatgc
    26641 aattaaagaa atggatcaag gttatgctga gagagaagaa agtattcaaa atcggattga
    26701 aaagttagca tatactgatc caatagatca aaaaaaactt gataaagcaa aagctgaatt
    26761 atctaaattg agaaatgaaa aaaataaaga agcgagagaa atattaacgc aagcgatagg
    26821 gttgaagagt caaacgagaa cacgagatgt agcaaaaaaa ttagactttg actttggaga
    26881 ttttgttgat tccgcaaata tgaaagctag taaagatttt ggcaatgatt ttatcggaat
    26941 tgtgaccgga caaagaacgc ctagaatata taaaaataga aacaaatatg gattactacc
    27001 ttttagaggt cgagatatta aaatccataa agatgataag ttttatattt atttacctaa
    27061 aggctttagg tttaaagtga atttaggtag caagccaaga aaagcaagag atgttgcttc
    27121 agttttacaa cacattatgg atggcgatta caaattaaat caaagtaatc ttatttttga
    27181 tggaagaaaa ttatttttta atctaacaat ttcttttgaa aaaagctata atgaaatgga
    27241 attcaagaaa ggtaatgaat tgatcgtaaa ctttgcagat ttgtgtagcg gtgaatgttt
    27301 aattaatgat aaatctgtaa tgtcttttgg tgatagccaa tatataagaa acttcaaaag
    27361 taaaatttta gcaatgaaat caaaagaaca aagccgagga gtcttttcta agggcggtca
    27421 tggtcgaaaa agaaaattag atcaagatcg atgggaagca ataaagaaaa gagaaagtaa
    27481 ttttgttaaa acttataata atcaatgttc tgcaaaaatc gttaaaactg ctattcgcaa
    27541 taaagctcaa attattcgta tcattgaacc agataaggct tatggcgaat gggcttacta
    27601 tcaattgaaa gaacaactag taaacaaagg aaaaagatat gggatagaag tcagaagtta
    27661 gtaaattaaa caatcgttga tttggcaacg aaatagtgag ggaaaatcca ctcactgaag
    27721 ggtaaactcc gtcaagttgt gtgaccagac ggaaggacga atgttgttga ggttttagaa
    27781 ccatataaaa cagcatgttt ctcaaatttt taggataagg gaaattgcta gcagtttgtt
    27841 ttagaaccat ataaaacagc atgtttctca aatgagtact ttaaattaat tgatggcgtt
    27901 cctgctttag aaccatataa aacaacatgg atttaaaaca agaagtaagt cagttctcct
    27961 tttactttag taaggaaaaa taaagtattc tcaataaaac ttaaaataag caaacctttg
    28021 ttttaatagc taatcgaagt aaaacccaaa gccatatgaa cccctgaatc agcgaaagtt
    28081 tatgatgatt tggattacta atagtttagg aattagatgg taaatctaaa ttaaacaatc
    28141 gttgatttgg caacgaaata gtgagggaaa atccactcac tgaagggtaa actccgtcaa
    28201 gttatgtgac cagacggaag gacgaatgtt gttgaggttt tagaaccata taaaacagca
    28261 tagttctcaa attgtttaaa atgattgtca agttaaaaaa atctgggatc atataaaata
    28321 gtatagttct caaatatgat tagaaaccct attaaagagc aatattaagc caacttactt
    28381 aaatcgttta attaagaaca ataatctctt ttagtcagac ttttagtctg acttttttgt
    28441 atttccaaaa tttattcttt acaaaaacga acatacgttt gtataattgc ttgtgaatgg
    28501 gggaataaaa atgtttgatt acagtagaga accggtgaat gactattttt taatcgacat
    28561 gaagtctttc tatgctagcg tggaatgtat tgaacgaaat ttagacccaa tgactacacc
    28621 gttaattgtc atgagtagat cggacaacac tggttcaggt ctgactctag cttcttcacc
    28681 tactgcaaag aaaaaatatc atattactaa tgttagtaga gctactgatt taccttatcc
    28741 attaccaaaa gaacttcata ttgttccacc acgaatgaac ttatatataa aaaaaaatat
    28801 ggaagttaat cagattttta gaaagtatgt cgcagatgaa gatttattga tttattcgat
    28861 tgatgaatca attttaaaag tgacaaaatc cttaaattta tttactagtt caggaacaag
    28921 aagcgagaga agaagagaat tagctaaaat aatcaaagat gagattcaga aagaaacagg
    28981 tctaatagca acagtcggaa taggagataa cccattatta gctaaacttg cactggacaa
    29041 tgaagcgaag agtagtaaag aatttatagc tgagtggact tatgagactg tacctgaaaa
    29101 aatatggaaa ataaacgaga tgactgactt ttggggcatt ggatcacgca tgaaaaaaag
    29161 attgaatcat atgggaatta tgagtataga agatttagca aattggaatc cctacacaat
    29221 taaaagtcag ttaggtgtaa tcggactcca gttatatttc cataccaatg gaattgatcg
    29281 tacggatatt gctattccac cagaaccaac caaagaaaag tcttatggga attcacaagt
    29341 tctaccaaaa gattatacga ggagagaaga agtagaacta gtagtcaagg agatggcaga
    29401 gcaagtagcc attcgaatta gacaacacaa ttgcaaaact ggttgtgtac gcttagcaat
    29461 tggtacgtca attttggaag aaaaatcagg tttctcgcac cagatgaaaa taccaattac
    29521 agataatacg aaagaattac aaaattattg tcttttttta ttcaataagt tttatgaggg
    29581 gcaggaagta cgacatatag gagtaactta tagcagatta gtatacacag attccctaca
    29641 attagattta tttagtgatc caagaaaaaa gattgctgaa gaaaatttgg ataggattat
    29701 tgataagatt agacaaaaat atggatttac agctattata catgcttcat ctatgttaga
    29761 aggtgcaaga agtattacga gaagcacttt aataggagga cacgcaggag gattaggagg
    29821 tttgcataat gattgaatat aacgatagag gacgtttaaa atggtcggga ttttatttgt
    29881 ctgatcatac tgaaagaatc aatgcagaca atgtacaacg tgctaatcat aatttggcaa
    29941 aagaacaaat gacgactgaa gagattgcga aaatactgag aactgctgtc ataaaaaata
    30001 gacccgtctg cattcaaaaa gaggaacgaa acgctgaagg ttactatcca gatgatgtag
    30061 taggaaaaat tattggatat gacgagcttg gtgtatttgt ggaagaagaa aaagttcact
    30121 atgatgaaat taggaatgtt tctttctcgg aattattaaa atggagtcag atatgaacat
    30181 tattaaaaaa tacgaattcg gttatattac gtatgaacag ctaatagagg aattatgggg
    30241 atatggtcaa caattaatta atgaagtggg gattgattgc ttttgtttct atattgaatc
    30301 cggagcagga taccataaga acagatacta tatatttcca tatccaccaa aatagtctct
    30361 tagatatttt taaattttct ccaaatactt tcaattattg tgaaatattt ccctttaatt
    30421 aatcaaattt tttataaaag taatatataa aataaattgc ttgaagtatt atatttagat
    30481 atcataattt atggaagaaa aattaattaa aaaggatgag attataatgg ggttactggt
    30541 tccgagagct aatacttata ctataccttt aacgaaacgc caattaattg ctaaaagata
    30601 tcaaaggatt actagagcca tcaatagaga gttttggaat tctgaaagtg atactgctca
    30661 cagtttatac gtaggttctt atgggagagg aacggcaatt agtactagtg atatagatat
    30721 aatagttgaa ttaccgatgg cagaatttga cagattcaaa aattatttat caaatggccc
    30781 gtctaagtta ttacaagtta taaaaaacgc atttcaggaa atactaccaa atagtgatat
    30841 tagagctgat ggacaagttg ttaaaataaa tttccatgat ggaatcaaat ttgaaatagt
    30901 tccagctttt aatgaaaaag attattgggg tgagtcaaaa gggtttattt atccagattc
    30961 aaacatggga gggaactgga aagctacaaa tcccaaaaaa gagcaagaag ccatgaaatt
    31021 aaagaataca aaaagtaata atttattata tgctacgtgt aaacattttc gacatgtacg
    31081 agatacagaa tttacgagtt atcatctttc aggaatagta attgacagtt tcgtatatga
    31141 ggctatggga aattggaaat tcgttgaaaa taacagtggg ggtcaaaaca ttagttctgt
    31201 atcttatgag acagctttat tagaatacta taactcacat aaagttatgg gcggtttaaa
    31261 tttatactct cctggaagca atcaatttgt taattctgat agtagtatca tatgtttaga
    31321 aaaagtatta aaaaaaatag ctttataaag ttttatggag gaacaacatg ggagacgaaa
    31381 aaaagcgctt ggaagttcaa ttacgtgaag catatggacg agtaacgtat acttacacca
    31441 ctcatttaaa gcaagccgat aatattattt ctgacaataa aattttaagg tgtcttcaaa
    31501 ttggtttgtc agcccttact actggagggg tacttagtac cattattact gataatagtg
    31561 agttaacctt tgttgctgga ttatgttcaa caatttctct tggattaaac ttatacttta
    31621 agaattttga tcttgctgaa gaagctaaaa atcatcaaat tgttgccgat aaattatggt
    31681 tgatcagaga agaatacgta agcttactaa cagatttaaa tcgtttaagt gttgaagata
    31741 ttgtgaaaaa aagagatcaa ttaatgaacg aaactcatga agcatataaa aatgccccta
    31801 aaacaaatag taaaagctat gaatcagctc agaaagcatt aaaatctgaa gaagagcaat
    31861 tttttacaga agaggaattg gataagatgt taccagagca tcttagaaaa aagagttcta
    31921 aatattgaac tcttttttta tgcttaaaat tctcttgtaa tttttctatt catctatatt
    31981 taagaagaaa tatgatgata gaaacatctc ctaaagccac cgcaagccgt tctaagcata
    32041 gcttagcccg acattgctag atatgctcat ctcaccatga acgtttccta aagccaccgc
    32101 aagccgtcct agccattgcc tagtccgaca tcgctagatt agtgcatctc gccataaaag
    32161 ttttctaaag ccaccgcaag ccgtcctagc cattgcctag tccgacatcg ctagattagt
    32221 gcatctcgcc ataaaagttt tctaaagcca ccgcaagccg tcctagccat tgcctagtcc
    32281 gacatcgcta gattagtgca tctcgccata aaagttttct aaagccaccg caagccgttc
    32341 taagcatagc ttagcccgac attgctagat atgctcatct caccatgaac gtttcctaaa
    32401 gccaccgcaa accgtcctag ccatcgccta gtccgatttt gcttgatcgg ttccatttct
    32461 gtttatttag tgggtcaaca ccgtaatctt aaatagccat cgcctattta agattactag
    32521 gtctgctttg aaggtaaaaa gtctttcatt cttcaagact tttaggcttt caagctacgc
    32581 ctgaaagcat tgaaattcat cgctctttat ttggttttgc catttataat tccttacgcc
    32641 cattatcttt ttcgcctgtc ataccttgtc agaagtgttt agagcttatc tcatctttgg
    32701 attaatacaa gtggcgtaaa tttttttcta aacgcaagcg ttacgaaaat aatttactta
    32761 aacacttgta ttaatccggc agataagaac gcattctaat cactcccgac aaggcaagac
    32821 aggcgaaaaa gataaataaa tgaacgaaag gaagaaacat aaaatgcaaa accaaatagc
    32881 gatgaagaaa aaaccacctc cctctaaaac aatccaaaca aaaaagaatt ttgaaaaacc
    32941 aaaaatcaaa aacagaaaga agcgttcaac atgagagata gaattttaga agttaatcaa
    33001 gaaatggtaa acaagttaac aaatatcagt gggaactatg aaaagtacga tggtacttta
    33061 cttgataact atatttttca cgatacacaa aatatcagaa ttggcagaag taaaccgaga
    33121 gcgttcattc tcatcaaaga gaaatatttg aatgaatgga atagcaccta tgaaatggtt
    33181 atgactgata actttgaaaa ggtattggat ttcagaaatg ctttgttggg ttctgatatg
    33241 ggaaatagca gtcttgaatc tattgaagtt gaaaacaaga ccgagaatga aggtgaagga
    33301 ttgattaaga atgtcttgaa aatcgaacaa aaatatatca gtgaagatag aagtttttat
    33361 agagtcacaa gctcggttgg tctagcaaac tttgctacta acttaattgg aaacaatgct
    33421 agtgaatctt tgctagtgat gtgtctgaat acaaagaatg aggtcattgc ttatagcgaa
    33481 gttttcaaag gtggaattaa tcaatcagta gcgataccaa gagagatttt tcaactagct
    33541 ttgctaaaca attcagcaaa aatcgcagta tcacataatc atccgagcgg atatgaattt
    33601 ccaagtgaaa atgacaaaag ttttactacg aatttgaaaa acgcaggaaa aacaattggt
    33661 attgaattgt tagaccatat catcgtaaca agtaaaaaca caagctatta ctcatttcaa
    33721 gaaaatggtt ctttataaaa attagagaaa agaggaaaac aagatgggcg aaagaacaca
    33781 agcgttaatt acagtcaaat atggtaacag caaaacgaaa gcaagcgtgt cgattcatta
    33841 tcagtggggg tatggtcgat atatgctgat ggacgtattg aatcaaggaa tttatttaat
    33901 caataaagca tacgacttac gagataaatc aagagaagaa ttagcaaaag gaatctttga
    33961 tagaagtaca ggagttatca attttgttac ctatgaatct ggagtgagtg cttatgatga
    34021 aacagggttt ggtgggcgct atgaacctta tcaagaaacg attgaagatg tgatgaagtt
    34081 ttcagataac aatgatggct atgctcattt agagatggtg ttcgatgaaa atggatttag
    34141 tctagatgat aaaaccagat tgcgattgtt tgattttgag ggtcaacaga taggactaaa
    34201 caagttttgt gaaagcggaa aagagtttgc tacgaaagat tttattaagg gatacagatt
    34261 attgctagaa aattatggaa taaacctaga agaagagagc gtgaaataag tgtttgaaaa
    34321 aagaaaagac cagttcccta atttagaagc gaaaagtcaa aaagtgattg atggtgaaat
    34381 gattgaatta ttccaaaaaa gttatggaac catagaaact accgcaagtt gcaaatacta
    34441 ctttcaagga aagagaaaca cgtttaaagg ctttgccgat tctgatgaag aattggcaaa
    34501 gaaaatcatt gattggcttg tcagcagtga acacgtagca agggaagata ccgttagaac
    34561 aacgattaat ggggaagtcc taacaggtac tagatgtgtt tgttgtaatc atgaatttgt
    34621 agaagatgaa ggttgctacc gaatcaatga atatggtgat tttatttgcc agagttgcca
    34681 cgagatagaa tttccaaccg aaagaagttg gtttgaatac atgctacaag attatgagat
    34741 ggaaaaagaa aatggtgaag tagttttttg taccaaagag gaattgagta agagaagcga
    34801 tgatgaaatt gaagattggt gtgtgtggtt agtaggggca gatgattgcg ttgcatatta
    34861 cacaacagca gaataggagt gtgaatcagc atggaaggaa tggattttct agatcatgaa
    34921 gatttggttg attttggtta tacatggaaa ggaatggtag gtattagcag aagtcttgct
    34981 aatgcctttt acgaaagaaa ttatgcagta tatgttcttt atgatgacga tacagaaagc
    35041 cttgttgacg aggaatataa attggattta gagaatgttt tatatggaat agaaaaggaa
    35101 gatttagcaa aatatatttt ttcatggttg ggtcaataag tgaaaggaag atagaaacat
    35161 gacaaaattt aaaagtaagg gtatgcaatg gtgttcttat tattatgttg gaaatgatga
    35221 agatttattg agaaaaaccc aacgaaaagg ttatccagat aagatattga aacaaatagg
    35281 ttattacatg ccagaagaga ggatagaaaa agaattttgt gaggttcttt ttgatagtga
    35341 acagctatta gttttgttaa cccaaaataa attgatttgc caatttgaaa ataattaaga
    35401 tgaggtggtg aaccatgaat gaagataaga tggtattaag tgaatatgct agattgagaa
    35461 ctattaattc tttagcatct catttaagtt gttctttcct aacaaaggtg ctaaaattaa
    35521 tttacaaatg tgaagatttt gtacaagaga agcacaaaga caagcctact gcgaataggc
    35581 ttgtcttttt ttcattcatt acgattccgt atgtgtgggt tagcgacaat ttgttcaatt
    35641 accgtatgat atagcatctt aatttgatta gattacttgc aatgtagttt attaagagta
    35701 taatataatc ataaatgtgg acaatttgat aaataatgag tgttttatac tcaaaaggag
    35761 tggatgaaat gaaggtcaaa aatccaactg aaaaaagcag aatccaagtc ggaatagaca
    35821 agaatttaaa agagaacgca gaaatgatat tggaagagct aggtttaaat cctacaacag
    35881 caataacaat tctttacaaa caagtagtcg ctagaggtga gtttccagta gagataaagc
    35941 taagtgaaga ggagaagcaa ggaatcagat tgcaacagct aactaaggac atgccagtag
    36001 atgttctaga tacagatgaa aagttagaag agtggttcga tgaagcctaa tgagatagta
    36061 acggtatatg ttgcatttac agataaaaat ggtggtaaaa gaagacctat tttggtggtt
    36121 agtgataaag aagatagagt agaattcttt ggaatcacaa gtcaatatga gaaaaaatca
    36181 gaccaaataa agagggtgta ttttcctatt gcagaatggg aaaaagcagg acttaaaaag
    36241 caatcatgga ttgacgtagg gtctttaaag gctattccca aaagccgaga gaacatatca
    36301 ttcaaaaagg ttggtatgtt atcagctagt gatacgaaaa agctaaatag gtttatcgag
    36361 aaattacaaa gaaaagtcta acaaggcttt tcttttttgt ttatatgtat attaagatat
    36421 acgtttatat aaacgtattt atgtatatga aaatatacgt aaatatatac gtggttataa
    36481 acattaataa aacgttaaga attgttaaat aatgatattt agtaatatac gaattataat
    36541 atatatttta tataaaatgt atgtaaataa ataaaatgtc tgacggatat tgaatagcaa
    36601 taaataacaa taattcagac ggatacggtg agtgaagtag cttgctacga acgaacaggc
    36661 agaatgaaac ggcagtttca ttagagcaag tcgcttgcga cgtagcgtta ccgctgatgt
    36721 aactggcaag ttacatagag agagtttgct tgcaaacgaa cgttacctat ttgaatagaa
    36781 ataaaaaata acagattagt gaattttgaa taggagccac aactcaacca accgcttgcg
    36841 gtgggtgagt cccacggagg aaaagacgaa cgaacggtcg cgt
//
LOCUS       DAZABD010000020        36569 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000020 DAZABD010000000
VERSION     DAZABD010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36569
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.020"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="ACHALG_001115"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="ACHALG_001115"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFN2586539.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="ACHALG_001116"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="ACHALG_001116"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFN2586540.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="ACHALG_001117"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="ACHALG_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFN2586541.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="ACHALG_001118"
     CDS             complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="ACHALG_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFN2586542.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="ACHALG_001119"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="ACHALG_001119"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFN2586543.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="ACHALG_001120"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="ACHALG_001120"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFN2586544.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="ACHALG_001121"
     CDS             complement(8053..8523)
                     /locus_tag="ACHALG_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 domain-containing RNA-binding protein"
                     /protein_id="HFN2586545.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="ACHALG_001122"
     CDS             complement(8569..8994)
                     /locus_tag="ACHALG_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HFN2586546.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="ACHALG_001123"
     CDS             complement(9061..9330)
                     /locus_tag="ACHALG_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFN2586547.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9341..10936)
                     /locus_tag="ACHALG_001124"
     CDS             complement(9341..10936)
                     /locus_tag="ACHALG_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFN2586548.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="ACHALG_001125"
     CDS             complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="ACHALG_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFN2586549.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14489..15049)
                     /gene="pth"
                     /locus_tag="ACHALG_001126"
     CDS             complement(14489..15049)
                     /gene="pth"
                     /locus_tag="ACHALG_001126"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFN2586550.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15402..16376
                     /locus_tag="ACHALG_001127"
     CDS             15402..16376
                     /locus_tag="ACHALG_001127"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFN2586551.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16546..17946
                     /locus_tag="ACHALG_001128"
     CDS             16546..17946
                     /locus_tag="ACHALG_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HFN2586552.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18024..18746)
                     /locus_tag="ACHALG_001129"
     CDS             complement(18024..18746)
                     /locus_tag="ACHALG_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN2586553.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18825..19331)
                     /locus_tag="ACHALG_001130"
     CDS             complement(18825..19331)
                     /locus_tag="ACHALG_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFN2586554.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19454..21253
                     /locus_tag="ACHALG_001131"
     CDS             19454..21253
                     /locus_tag="ACHALG_001131"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HFN2586555.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21321..22895)
                     /locus_tag="ACHALG_001132"
     CDS             complement(21321..22895)
                     /locus_tag="ACHALG_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFN2586556.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="ACHALG_001133"
     CDS             complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="ACHALG_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFN2586557.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="ACHALG_001134"
     CDS             complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="ACHALG_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFN2586558.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(24672..25310)
                     /locus_tag="ACHALG_001135"
     CDS             complement(24672..25310)
                     /locus_tag="ACHALG_001135"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HFN2586559.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25311..25961)
                     /gene="rpe"
                     /locus_tag="ACHALG_001136"
     CDS             complement(25311..25961)
                     /gene="rpe"
                     /locus_tag="ACHALG_001136"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFN2586560.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="ACHALG_001137"
     CDS             complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="ACHALG_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFN2586561.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="ACHALG_001138"
     CDS             complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="ACHALG_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HFN2586562.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29104..29844)
                     /locus_tag="ACHALG_001139"
     CDS             complement(29104..29844)
                     /locus_tag="ACHALG_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HFN2586563.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="ACHALG_001140"
     CDS             complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="ACHALG_001140"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFN2586564.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31215..32153)
                     /gene="fmt"
                     /locus_tag="ACHALG_001141"
     CDS             complement(31215..32153)
                     /gene="fmt"
                     /locus_tag="ACHALG_001141"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFN2586565.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32146..32637)
                     /gene="def"
                     /locus_tag="ACHALG_001142"
     CDS             complement(32146..32637)
                     /gene="def"
                     /locus_tag="ACHALG_001142"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFN2586566.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(32652..35060)
                     /gene="priA"
                     /locus_tag="ACHALG_001143"
     CDS             complement(32652..35060)
                     /gene="priA"
                     /locus_tag="ACHALG_001143"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFN2586567.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35313..35618)
                     /gene="rpoZ"
                     /locus_tag="ACHALG_001144"
     CDS             complement(35313..35618)
                     /gene="rpoZ"
                     /locus_tag="ACHALG_001144"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFN2586568.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="ACHALG_001145"
     CDS             complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="ACHALG_001145"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFN2586569.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYINKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
BASE COUNT         9886 a         7989 c         6706 g        11988 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gttttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaagc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc tcctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatgggat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttat
     9361 ttaatcgcag gattttctta ccaaaaggca acattagcca ttctcgtact gtaaacagac
     9421 gaagacgaat cactgcataa atgaacacag aagccccgat cccaactcct atcacgctga
     9481 caaaaaaagc agtggaacga tgagttacag gaccaaatac tatattcaat acaccataat
     9541 acagcagcag tactactgtc atacaaagaa ggctgaacat caacttccgt ccaaaatgcc
     9601 gttctttcca aaaagcattt agctcttttg tttcaattcg aacaaaatac caaagcgtca
     9661 cacctaatcc taaaagggta gacaggcttg cacctgctgt tcctaataaa ccggtcagaa
     9721 aagaagtagt caatcctttt accaatagtc cccatcctgc gccttttaaa gaagggcgaa
     9781 acgagttctt gctttgagca atactttgat aagcttggat gactgcagtg aaagcaatgg
     9841 aaaaaacaaa taatacaagc gctgcatttc ccgcataatc tttaaacaac gcataattga
     9901 tataagggag cagcaaggct aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc
     9961 taagatagat tttggctgtc tgtaaaaact gacgatagat ccggttggtc aaatgacgag
    10021 tcaacgcagg caggaatgtc gcacttaatg cagtggcaat cactaaacct aattgtacta
    10081 aaggctgacc tcgatcatag accccttttg ctatcttcgc accgtattct gacagtccgt
    10141 aaacttccaa agcatttttt acaaagaaag agtcgatcag ctggaaaaag ataagcaatc
    10201 cactataaat cgaaaccagt ccgccttcta ccagaaaacg ccggatcaag ggctttgacg
    10261 gctgtttgaa caatggaaac cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat
    10321 agtagtgaag gatcccatat gcgcataaac cgccaaaaac agctccactc attgctgccg
    10381 tccctgtttg gtacaccgtc catccaagct ttctaaaagc aaacgcacta cagacaatca
    10441 caaaaacacg aacgaactgc tcaactactt gagaaacagc agttggttcc atcaagaatc
    10501 ttccttggaa attccctcga taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa
    10561 ctacttggat cagcggtttc aattgtatgt ctcccatcat ccaagcaatc cagctgctaa
    10621 atgcaaagac aaacaaccac aaaaagacac ctgtccaaaa tactaaggga taaagatttt
    10681 gcaaagcttg ctttcgctcg aaaggattct ttctttctgc aacgtacttt gatatgaact
    10741 gcggaagacc ggataaagct aaagtcattg ctatcccata aattggataa acttgctgat
    10801 aaacataaaa accttcgtcg ccgactaagt tttggagggg aattcggtaa agggcactta
    10861 atacttttgc tatgaaagaa gcaatcgtca atacaaatgc tccttgcatg acgcgccgca
    10921 tttctttatg atccaaatca ttgctccttc ctacgctgac agatattttt gctcacgcaa
    10981 cgctttgaca aacttttgga tttcctgaag ccatactgct tcttccattt ggttggggat
    11041 cgtcaagcgt atcaccatct gttctttttc aacgcctaga ctggctttta gttttgtcgc
    11101 tgtcagtgct tcgaataact gttccacact atagcgtttt gttccaactt tacttagagt
    11161 aaagactata tgcgcctcct gtttccttat cgtttctagg agcgctcgat cgccatccat
    11221 tttgatctgc ccaattgtca agagatgtgc tacttcttct ggatattctc caaaacggtc
    11281 gagcagatca tcttctagtt catctagcat ttcttgcgaa tcaagttcac gaattcgttt
    11341 atagatctcg attttctgcc gttgatctgc cacgtatgtt tcaggtagat acgcatcgac
    11401 accgaggtcg atctcaacag tggtcttctc cacttgacta ttctttcctt gtttacgaga
    11461 aacagcttcg cttagcattt gagaatacat atcgaaaccg actgcatcaa taaatccatg
    11521 ctgctgagca cctaagagat tacctgcacc gcgaatggaa agatcccgca tcgcaatctt
    11581 gaatccagat cccagttctg taaaatcttt gattgcctga agacgttttt cactgacttc
    11641 gttaaggatc ttctgctgct catacataaa gtaagcatat gcgacacggc tgcttcttcc
    11701 tacccgccct cgcaattggt ataacgtgga taatcccata taatcagcgt tctcaacgaa
    11761 caacgtattt gcgttcggga tatctactcc tgtttcaatg atggttgtag tgacaagaac
    11821 atcatattga ccttcaataa attcaaaaag cgtattttct aattgcgcct ctgtcatttg
    11881 cccatgagca taaccgatcc gtgcttccgg aacgagttct tggatttctt ctactttttg
    11941 ttcgatcgtt tctacccgat tatataaata gaatacttgt ccacctcgtc ccatttctcg
    12001 atggattgct tcgcgaatag ccccaggatt tttttccata acatacgttt ggatcggata
    12061 acgattcgca ggcggtgttt cgattactga aagatcccgc acgcctaaca tcgacatatg
    12121 caaagttctc ggaatcggtg ttgcagtcag agtcaaaaca tccacttgtg cacgtaactg
    12181 tttcaatcgt tccttatgtt ttacaccaaa acgttgttct tcgtcaatga tcagtagacc
    12241 caaatcgcta aattcaatat cttttgacaa aattcggtgg gttcctacaa caatatcaac
    12301 ttgacctgta cgcagttgtt cgatggtttc tttttgttgc tttcttgttc tgaaacgact
    12361 taatagcccg atatttacgg ggaaaccttc aaatctttcc agcatcgttt catagtgctg
    12421 ctgtgccaag atcgttgttg gaactaaaaa ggctacttgt ttgctttcct ttatcgcttt
    12481 aaaggccgct cgaagcgcta cttctgtttt cccataacca acatcaccta cgagtagtcg
    12541 atccattggt ttttcttttt ccatatcccg tttgatctcc gctgcacttc tcaactggtc
    12601 atcagtttca gagtagggga aggcattttc gaattctttt tggtaggcgt catcaggtcc
    12661 aaaagcatat cctttttctg actctcttgc ggcatataat ttgattagat cgtcagcgat
    12721 atcctcgatt tttgaagaga cttttcgctt ggttttcgtc cattcacttc cgcctagctt
    12781 attgatgcga ggcgctttgg cttcagaggc gacatatttt tggatcagat tcagttgagt
    12841 aacaggaata aacagtttgt cgtcgttttg atacaaaata gtcatatagt cttggtgaac
    12901 gccatctact tctagcgttt ccatcccaat gtatttcccg ataccatgat tggcatggac
    12961 aacgtaatct cctgctttta actcattata gcttttcaat cgctctgcgt ttgttactgt
    13021 ttgacgtctc gtttgttttt tcgtcgtttt ttggaaaatt tctttttctg tgatagctac
    13081 tattttttct tgaggaagtt caaaaccagc ctgcaaagct ccttcaacta attgtacctg
    13141 tcctctgatc aatgcatttt tttctgtttc tactactaat atgtcttcat ctcgaagcat
    13201 ttgttctgct ttgcgtattc tgtcctttgt cggaataaag atcacaacag tctgtttttg
    13261 cttgcgccaa cggtccattt ctgttttcag caatggcatt tgtccgaaga actgttgcat
    13321 cgtccgatat tgaaaattat ggattgcttg gaaacgaaga tttcccatcc ctttttggaa
    13381 gagtgagaaa aaggttgtgg tgaacttttc cttctgcacg attttttgta tatctgctga
    13441 aaatgtctgc tcagaaaaca cccgcaattc gctgattttt tgagtctgcc attctgccgc
    13501 ttccctttcg atttcccgat ttgtttccat gattcgctga taatcatcaa cgactagtaa
    13561 actatttgaa gggaaataat ccaagatcgt cacagattct tggtaaagaa gatctgagta
    13621 gaattttgct tcttctgtcg ggacacctgc ttgccagctt gaagtcagct gaccgaagta
    13681 atcttgcaaa aactcgcggt ctgcttcttc tgtggtcgca gcaagccgtt tttctagcat
    13741 tttttgcatt tgctctattc catgttctag atcctctggt ccgtagactt gttcactcgt
    13801 tggggaaacc cagatttctt ccgttttttc gattgagcgt tgtgtttctg cttcaaaata
    13861 ccgcatagaa tctatctcta catcaaatag ttctacacga acaggatagg ttgtcgttaa
    13921 cgggtaaata tcgataatgc ttcctcgaat actgaattct cccggttttg cgaccatctc
    13981 ttctcgtaca tagcccatca aagtcaattg gcgaggaaga ttttccacgt ctatctcatc
    14041 accaatatgc caattcaatt gcgcctgtag ccaagttttc ttattcggca gtcgtttgtg
    14101 caaagatgcg actggcaaca aatagatacc tgcttttttt tcttgaagtg cgttaagcgt
    14161 tgcaactctc tcgcctcgcg cttctggaga agaaaaggcc atttcagcag aaagcacttc
    14221 atccactgga aataaatgga tctgatcttg cggaatcaca tgttgcagat cttctgctaa
    14281 ctgattcgca taatacaaat ttggcatcag aataatcatg tttttatttg ttttcttaaa
    14341 cgctcgagca aatagaagtg ttttcgctga gcccgctaat cctgtgatta gttgtctagg
    14401 cattttttct tctaaattgc tgacccattc ttttacttgc ggatcttttc cgactaattg
    14461 aataatatcc attccttttc ctccttcctc aattgaatcg attcatcgca tccacgaacg
    14521 aatgcccttc tatgagatat tcgactgctt taacagattg acacatgctt tcttctatca
    14581 aaggctgatt ttctttgctg aaaggactaa gcacatgctg gactactgtt tttttccctt
    14641 ctggcctgcc gattcccacc ttgattcgat caaagacatt cgtattcaaa tgggaaataa
    14701 tacttttgat accgttatgc ccgccggcac ttcccttttg acggagccgg atttttccca
    14761 ctgccagatc caaatcatcg tagattacga ctaattcctc tggataaatc ccaaaataag
    14821 tcattagtgg gccgactgca cgaccagatt cattcatgaa tgtttgtggt ttgactaaaa
    14881 ggatcttttc tccattatga aaaaattcgg caacttctgc ttcgaacgga ttttttttga
    14941 aagtagcttg atgctcgcgt gcaattttat ccacgaccat aaagccgata ttgtgttttg
    15001 tatattggta tttggtacct gggtttccta acccgacgat cattttcatt tttgttttcc
    15061 acctaattct aaaaagcagc acaaaaggct ggactgctaa ttagcggtcc agtccatcga
    15121 gcttttttct tttttatact tgtcaatgca tagtataaca caaaacaagg cgtttcggct
    15181 aaaagagaat cgttaataaa tgataaagaa attaaaaatt tcttcaaagt ttaatgaaat
    15241 aattgatata taaaaatagt gaaataacgc acatgtaagc ttttacatat aatacaacct
    15301 tgttatctgt tatattttat agccaaatag cgtgacatgg ggcttgagaa atgttagcat
    15361 aagcatggga aaaaacaaat caatggaagg attgatacta aatgactgca gctgcagaaa
    15421 aaaaagatca tcaaaaagtt attcttgttg gagacggcgc cgtaggttct agctatgcct
    15481 ttgctctagt tactcaaaat atcgctcaag aagtaggtat tgtggatatc aatactgcta
    15541 aaactgaagg agacgcaatt gacttgtctc acgctttggc atttacttca cctaaaaaaa
    15601 tctattctgc tacttacgca gacgcacatg atgctgactt agttgttatt acagctggcg
    15661 caccacaaaa accaggtgaa actcgtttag acttagtaaa caaaaaccta aaaatcaacc
    15721 gcgatgttgt cacacaaatc gttgaatcag gatttaacgg tatcttttta gttgccgcta
    15781 acccagtaga tattttgact tactcaactt ggaaattctc aggtttccct aaagaacgcg
    15841 taatcggttc aggtacctct cttgactcag ctcgtttccg tcaagcactt gcggaattag
    15901 ttgacgttga tgcacgtaac gtccatgctt acatcttagg tgaacacgga gattcagaat
    15961 tcccagtttg gtcacatgca aatgttgccg gcttgcaaat ctatgaatgg gtaaaaaaca
    16021 acccagaaat cgatgaagaa gctatggtta acctattctt tggcgtacgt gacgctgctt
    16081 acacaattat cgagaaaaaa ggcgctactt tctatggtat cgcagtagca ttagcacgta
    16141 tcactcgtgc catcttagat gatgaaaatg ctgttcttcc tttatcagta ttcatgaatg
    16201 gcgaatatgg attgaacgac atctacattg gtgcacctgc tgttatcaac cgtcaaggta
    16261 tccaaaaagt aatcgaaatt cctttaagtg acagcgagca agaccgtatg gctgcttctg
    16321 ctaagcaatt aaaagaaatt ctagatgaag cttttgctaa actagacgct gaataatctt
    16381 tgaacatgac ggaaaacatc tattcggtgt tttccgtttt tttattttat cacccttatg
    16441 gtttctaaat tttttcataa agtagagata accttcgtct tttcctctaa attatgctat
    16501 catgagaccg ttccacccta aacttttact taaggagttg tcgatatgac aagatcttcg
    16561 catcgaaaaa aatcgaaaca tgttgctttg ctttcagttc ttggtgttct cttagtgctg
    16621 atcagctttt atacctatcg cagtacatac tacacaaagc attttttgcc gaatacagaa
    16681 ataaatggaa taaatgtcag caatctaaca gtcgaaaaag caaatgaaaa attaaaagaa
    16741 gcttattctg ataaaaaact ggctattaaa gaagatggaa aagtctggca ggaagtacca
    16801 aaatccgagc tcggctacaa agatgatttc acttcggagc tttctccgat cctaaatgaa
    16861 caaaacggat ggacttgggg aatgacttat gtctcagcgg cagaaaaaca agaaatagat
    16921 cctgtcagtc aaaaccacca aaaactagac actaccgttc agacattgac aactaagctg
    16981 actgatttga acaaagaccg gacacctact actgatgcaa cgatcgaaaa agcaggtgac
    17041 tctttcgtta tcaagcctga agtcaatgga aatacgatcg acgtcgatgc tgcggtcaaa
    17101 caattaaaaa gtgcggtaaa ctctggtaaa gatacaattg aattgactga attcaaagaa
    17161 aaaccaaaag taacatctga agacagctca ctgaaagaac aactcgcatc catgaatgct
    17221 atcgcaaatg taaaagctac ttacagtata aacggtgaga ctttccagat cccatcaagt
    17281 gatattatga gttggctgac ctacaatgat ggaaaagtcg atttagatac agaacaagta
    17341 cgtcaatatg taacagattt aggcacaaag tataacacaa gtacgaatga cacaaaattt
    17401 aaaagtacaa aaagaggaga agttacggtc ccagttggca cttacagttg gacgatccaa
    17461 acagacagtg agacagaagc attgaaaaaa gcgattctgg caggccaaga ttttactcgt
    17521 tcacccattg tgcaaggtgg cacaaccgct gatcatccat tgatcgaaga tacgtatatc
    17581 gaagttgatc tggagaacca gcatatgtgg tattacaaag acggaaaagt ggcccttgaa
    17641 acagatatcg tttccggaaa gccaactaca cctacacctg ctggagtctt ctatgtttgg
    17701 aataaagaag aagatgcaac tttaaaagga acaaatgacg atggaacacc ttacgagagt
    17761 ccggtaaact actggatgcc gattgactgg acaggtgttg gtatccacga ttcagactgg
    17821 caacctgaat acggtggaga cttatggaaa acccgcggtt cacacggctg catcaacacc
    17881 ccaccaagtg taatgaagga attattcggg atggtcgaaa aaggaacgcc tgtattggtc
    17941 ttctaacaaa aataatatat aaaaagaaag cacccgtacg attggtacag gtgctttctt
    18001 tttattatct aacaatgtgc tacttacatt tcagcagcag taaagacttc tgaaacatca
    18061 tcatcgtctt ctagcttatc tactagacgt tccaattgct ctttttgttc ctcatttaaa
    18121 tcaatcgtcg tttgtggaat catcgtcaat tctgcttgcg ccaaagtgaa ctctttttcc
    18181 aactcatctc gtacagcagc aaaatcttct ggtgcagtat aaatctcgaa tacttcagga
    18241 gaagtttgca gatcttctgc ccccgcctct aatacatcct caaacatcgt ctcttcatca
    18301 acagtcaacc catcgcgttc aatagcgata tatcctttgc ggtcaaacaa gtaactgact
    18361 gaacccgttt ctcccagtga tccaccgttt cgagtaaaag ctactcggac atttgtcgct
    18421 gtccgattac ggttatctgt caatgcatgg actaagattc ctactccacc cggaccatat
    18481 ccttcatacg tgatttcatc gtaattcgtt tcatctacgg cactcgtagc ctttttaatc
    18541 gcccgttcca cattgtcgtt aggcatattt gcagcttttg ctttgtccac aacaagccgc
    18601 aaagctggat tcatagatgg atcaggaccg cctgctttag ctgccatata aatttcacga
    18661 gaaagtttct gaaagacttt ccctcgtttt gcatcttggg cattttttct tccttggata
    18721 ttatgccatt tactatgtcc tgacatgatt tttcctccct tgttcttttg agaatacact
    18781 tctattctag tactatttca caaaaaaaca aagccacagc caatctataa acgcttcttg
    18841 aatgccgcaa aaagcacaca taatatcaca gcagtcagag caccgatcga atccaacatg
    18901 acatcttgga acataggtgt tcgtccaccg gtcatcatct gatggaattc gtccaaacct
    18961 gcgtaccctg ttgctgaaag ccaagcaagg atcgctgtca gccaccaaat ttttagcttg
    19021 gggaaaacac ctaaaaacaa gctgcctccc aaaataaaat aggtaccgaa atgtgcgccc
    19081 ttccttaaaa agaactctaa aaatttggcg tagcccagat ggtcgatact caccatactt
    19141 ccaccataat taaaacgtat acccgctaat gtgtctttaa atggctgatt aggcagccag
    19201 ctttctaatc gcgaaacttg tgattgctgt ccgtaagttt gagaagaact gtaaaataag
    19261 attataacga ctatcagtgc gattactaaa taaatatttc cgtttttcat ttgttttttt
    19321 gaagaattca taagtcacct ctgtatttta tcttagactt tttttgaaga aacaacaatc
    19381 attttcatag agacttcttc cataaaaaag tgctatactt aattttgtat tacaatatga
    19441 agggagtttt ttcatgagtt attcattaaa ttggaatcta gattctattt tttccggagg
    19501 cagtcattct gatgctttga accaacggat gaagcaattg gaagaccaaa tgaacgagta
    19561 ttatcaccgc gtaacaaaat ggtcacctag ttctgacaat gcagagcagt taaacgctat
    19621 tcttcaattg caagaaacga ttacgaatgg ttttacccaa tgcagcagct atattactgc
    19681 attgctttct gccaatgtca atgattcaga tgcaaaggtc ttatcaggca agctttacgc
    19741 aatgctgcct cgtctgcaat cagccgaaac tgtattgtca aagaaatttg cagaaatatc
    19801 tgacaatgat tggaatcaga tgctttctca atcttcgttt gaaacaattg cttttcgttt
    19861 aaacgagatt cgtcgtgatg gctcacaatt attatcagaa gctgaagaaa acattataaa
    19921 tacgctttct ttagatggtc taaatgcttg gagcagtcat tatgatacga ttgtagccag
    19981 catttcgatt cccttcgaac aagatggaca agtagtcgaa ttatctgctg gacaagcctt
    20041 caataagatg atgggcgatc cagatccaaa agtcagagaa acacttttcg cagcatggga
    20101 agaagcctgg aaagaaaagg cacccctctt cacggatact ttgaatcatt tagatggttt
    20161 tagactttct gactatgaac ttcatggtgt cacagatttt ctacaaaaac cattagagta
    20221 caatcggttg aagaaagaaa cattgtctgt catgtgggat acaatccaga aaaacaaaca
    20281 accccttgtt aattacttga ctagaaaagc taatcttttc ggtaaagaaa aaatggaatg
    20341 gcaagatcaa gatgcaccta ttattttagg cgatttgaaa gaaaaaacct ttagttttga
    20401 cgaagcagca gcatttatta tcgaaaactt taacaaattc agtccaaaaa tggctaaatt
    20461 tgcccaatct gcttttgaaa agagctggat cgaagcagaa gatcgtcctg ggaaacgtcc
    20521 tggtggttat tgtactgagt taccagaaac aaaagaatca aggatcttta tgacctacag
    20581 taattctgtt aatgaggttg cgacacttgc tcatgaacta ggacatgctt tccatagcag
    20641 cacgatgtgg gatttacctt ctttaaatcg cgaatatgcc atgaatgtcg cagaaacagc
    20701 aagtacattc gcagaactga tcgtagccga tgctacatta aaagtcgcaa aaacaaaaga
    20761 agaaaaaatc aatttgctag atacaaaact gcaaaatgct ttagctatgt tcatgaatat
    20821 ccattcccga ttcatttttg aaaatcgatt ctatgaagca cggcaagaag gattagtctc
    20881 agaagataag atcacagaga tgatggttga ggcgcaaaaa gaaggctatc aaggagcttt
    20941 agctacttac catccatatt tctgggctgc aaaattgcat ttctttattg atgacgtacc
    21001 gttttataac ttcccataca catttggcta cctctttagt ttgggcatct atgcttacgc
    21061 caaccaaaag ggagcaaatt ttgaagaaga atatatccat ttactacgcg atactgcctc
    21121 aatgacgacg gaagatcttg cgaagaaaca tttgaatgtc gatcttaccc aaccagattt
    21181 ttggcaagca ggtatcgaca tggtgactga agatatccaa acgttcatgg aattgacaga
    21241 agaatttatc tgatttctat tttatcacaa taaaaaaaga gttagtccag aatgggctaa
    21301 ctctttttga ttaatcattc ttacatgctg acagcaaagt caggggcaaa ccattgaact
    21361 gaaccaactt ttacgctttc gcctggttga ggagcatgga tatattgtcc tccgcctaaa
    21421 gcaatcgcta cgtggtaagt tccgccttgt gacccccaga ataacaagtc tccagctttt
    21481 gcttgagaaa ctgaaatttt tgttccagct gattcttgag gaactgtcca accaccgatg
    21541 tcacgaccag ttacttgcat gtaaacatag cgtgtgaacc ctgagcagtc aaagccactt
    21601 ggatctttac cgccccaaac atatggagta ccaatatatt tgtaagcttc tgctacgatt
    21661 gctgcaccat tcacacttcc agaaggagct ggagcaggtg ttacaggttt tggttgttca
    21721 ggtgttggtg ttggcgttgg agccggagta ctacttcctg tgccgtttcc tgtatcaaca
    21781 ctttgatctg ttgatggtgt tgtaggcgca ggtgttgttg attcttctgt tgttgaagat
    21841 tcaggcactg ttgttgattc ttctgttgct gaagattcag gcactgttgt tgattcttct
    21901 gttgctgaag attcaggcgc tgttgttgat tcttctgttg ctgaagattc aggcgctgtt
    21961 gattcttctg ttgaagattc aggtgttgtt gattcttctg ttgctgaaga ttgtgtagct
    22021 gatgatgatt cttcagtcat tgaagattgt gtagctgatg attcttctgt tgctgagctt
    22081 tgtgcagtag atgaagcttg agtagcttgt gcttcagcct ctgcttgttc gcgtgcttct
    22141 ttttcagctt tttcttgtgc agcttgttga cgagcttgtt ctgctagacg ttgttgttca
    22201 cgaatacgag cttgctctgc ttcagcttct gctttttgac ggttcaagtc agctttcttg
    22261 tcttcagcag ttgcttgttc agcagctaat gaagttttca gtacgttcaa atcagcttgt
    22321 ttagataaaa gatcgccttt ttgagattcc aaagcagctt ggttttcagc taattctttt
    22381 agtttagcat cgttttcagc ttttttgtct tctacagctt ttttatcttg tttttgttgt
    22441 tccatcaagt cgttgtttgc ttttaccatc gttgtcattg cttgaacgcg tccaatagca
    22501 tctgctaatg aatcagcatt caatacagca tcgatgtagt ttgagcttgt gttgcttact
    22561 tgtgcttcgc gtgcttgttt ttggattgta tcttcacgtt tttcaatacg ttcttgtaaa
    22621 tctgcaatgt cttttactaa ttgtgcagat tcttgacgca atgtatcttg ttttgctaat
    22681 aggtcttgtg cttgtgtgtt gatttcagaa acttggcttt ctaaggcatc gatttgtgat
    22741 tgtgcatctg cttgttgatt tttcaagtct gcaatctttt gatcttgctg ttgtatctga
    22801 gaatcaaaat cgtccgccgc ggcagcgatc ggtgatgcta cggcagtcaa ggtcattgaa
    22861 cataccatta ctgctgatat taaactcttt ttcactcttc attcctccga ctggcttaat
    22921 ttaataaata tctaaatata tttaagtctt tcttaacgat ataaaaattg taccatagca
    22981 gtgtgtcaac gatataatag ttatgttaca aaaatgtttc aaaacaactt ttaaaaatga
    23041 caaaactgtt cgtttcagaa aatgatcatg aaaaagccgc gttaagtcta gcatttctta
    23101 agataacaac gattcttttt cgctttcgac ctaaaaataa aaaaagaaat tattcatcac
    23161 tgaataattt cttaaaggga aaaatgaata atacaaatat aatcatgttc aacagcagtg
    23221 taggtcctaa aaaccttgta ataaaatacg tgttatttac tactgctaat ttaaaaatca
    23281 actggaccac catcgtaaac aattcataac ccgtgacaaa gatgatcata ctgaaaaatt
    23341 cagtaaagat gttgacatgg ataacgtctt tcattacata catcaaccat acaattaaag
    23401 ggagagctac tgcatagata ccgataacgc caatatagta agcgtcatag atagccccga
    23461 gaacaatcgt ggtaatcagt aaataacgtt tttcaaacgc catactgcaa cataataatg
    23521 ccaatatcag aaagtgggca tttgacatat aggtgccttt cgaccactcc cctagcattc
    23581 ttgttaaatg accatctatc aacatcaaaa agaacaaaac gattggcaga tagtacttca
    23641 ttgtttcttt cttcaacatg gactaatcct ccgctagccg ttggacaatc gtcacaactg
    23701 acacgtcata catttctgca taaggtttca cataaacttc ccgatccagc ccataactat
    23761 ctggtttcgt tttaataacc gtaccgatcg gcaagttagc tggagaattc cctccaagac
    23821 cggatgtctg gacaacatcc ccttctttga tatccgtatc accagttaat tgggtcacca
    23881 ctaaggcatg gagcttttca tcatagtttt taagcaaacc aaacgcttcg ccattagccg
    23941 aagatacccg tactggaaaa tgattggagc tttcattaga ggatgacagt aattcgattt
    24001 tagacgaagc cgtattgacc tcgatcactc ggccaatcaa tcctttttgc gacatgactg
    24061 ccatatttgc ttcgataccg tctttagaac ccttgtctac gatcagcatg tcctgccaac
    24121 tgtcaggcga acgagtaatg acatttgccg ttactttttc gtaactagtc agcgtctgat
    24181 tcaactcaag ttcttttttc aacgcttcga tttctctctc gtaattcttg ttttgctgtg
    24241 aaacgtcatc atagctatcg attttttctt ttagccgctc attttcggca taagttgaaa
    24301 aaagattatg gacagaatct actccatttc gtacccaccg tactggggca gagattgttt
    24361 tatcaaccat tgcaacactg tcattaacga tggattggaa gaaattgggc tttccttcat
    24421 tcgctcgctg cgcagcggtc acacttagta tcgtcaccac gatgataaca ataaccaagg
    24481 taataataat atttttatta ggattgaact tcttcacaaa gacacctcga attaattaga
    24541 ctcttgtgtt caattttatc actaaatgcc gtgaaacaaa aggtaagttg ctcctcttaa
    24601 gctttttttt agcaaataat agattctgtc atttttagcc tgaaagacaa atttttttat
    24661 ttgtttatgg atcaatcttt gctttgaata actgcaatca taccatctga aaaagaaaat
    24721 tctgcggtat ctgaaagaaa ttcgttactt gcataagatg tcggatattc tacttcttta
    24781 ttttccaata catacttgct gccttttaat gtaagattcg agacgggagt caagcagcaa
    24841 taagctaaat acttcatatc tggtatctgt cttactatat gctctccagg caaataataa
    24901 gacaaataat tttgacaatc tctgataata agctgatggg caaagggttt gaagcgaggt
    24961 tccataccta accagagatt ggctaaaaga tgatccaatc ttccgcccgt agctccaatc
    25021 aaagtgactt ccgcttgcgg gtaacgttca aatgttttta agatacctaa ctgtgtatct
    25081 gtatcgtctt tttctgcagg ggaacgggta tactctttgg cttttgcaaa tacttccttc
    25141 tgctcttcgt tcgacaatga atcaaaatcg cccactgcca gatctactgg gtagcctgct
    25201 tgaagcaggt acagcgcacc acggtctatc ccgacatagg catcgtattt ggacagcaga
    25261 gactgcggcc agttttgcgg attacctcca gcagctaata aaattttcat ttagtctaat
    25321 gcctctttta atcgttcgat ttgtccgact ggattttcgg ctccatagat atatgatcct
    25381 gcaacgaaga catccgcccc tgcatcttta cagatttttg ctgtttctgg aacgattccg
    25441 ccatctactt cgattgcata agtccaattg ttctctctac gtagatcagc gagttccgta
    25501 attttttcta ctgtttcttc aataaagctt tgtccgccga acccaggatt gacagtcatg
    25561 actagtactt gatcagccaa aggcaaaaca tgtttgatca tggagactgg tgttcctgga
    25621 ttgataacta ctccagcttt aactcctaag ctttttgtca tttgaagtgc ccgatgaata
    25681 tgaggagttg cttcgacgtg gacagtaatg atatctgctc cagcttttgc aaatgctgga
    25741 atgtagtttt ctggatcaac gatcatcaga tggacgtcta acggtaattt agtaacagga
    25801 cggattgcct gaacaatatt tggccctaat gtgatatttg ggacaaattg gccatccatt
    25861 acatctacgt ggatgtaatc tgcgcctcct ttttccacta actcaatgtc tctttgtaaa
    25921 ttggcaaaat ctgcactcaa aatggatggt gctatcttca ttcttgtttc ctcctatttt
    25981 cgtttacctt ctttttttgc atacattggt tttctttttt cgatttcttg taagaattgc
    26041 aaataattgt cgtaacgtgt ctgtgcgata ccacctgttt ctactctatg ttttacttcg
    26101 cagccaggtt ctttagcatg catgcactct ctaaatttgc aatggacaga agcttctaca
    26161 aattcaggaa attgtctcgg cagttctcct gcttcaattg tcaaaaagtc aatcgagcta
    26221 aatcctggtg tatctgctac taacccgcca tagataggaa tcagttccac atgacgtgtt
    26281 gtatgccgtc ctcttccaag tgcatcagaa atctctcctg ttgctaaatt cagttccggt
    26341 gatatttgat tcagtagcgt cgattttcct gctccagact gacccataaa gaccgatagc
    26401 cgttctggga agtaacggat cagttctgtc gtatcacccg tatgattggg tacaatcacc
    26461 ggatagccta ttttagtata ggtttgcttg atctcttcga ttttttctgg gtcgtctgac
    26521 aagtcggtct ttgtcaaaaa aataatcggt tcgatcgact cgtattccaa ggtaactaga
    26581 aagcggtcaa gtagattata agaaaatgca ggctctacga tacttgtgac aatgattcca
    26641 agatcaacat ttgtgactgg tgggcgaact aattgatttt ttctcggcaa gatttccaga
    26701 agatagccgt ctgtttgatt ttcactctca aagatcacct gatctccgac aagcggcgtg
    26761 attttccgat tccggaaatt tcctcttcct cttgtttgat aggtctctcc gtttgcgtat
    26821 acatagtaaa aaccgctgat tgcttttctg atttgtcctt tgataagtgc cacctccgtt
    26881 tttgtttatt ttaccatacg aagaggaaag taggtgttat tccatgcaaa cttctcaaag
    26941 tatatggctt gaaaattgat caaaagagat agaaacacaa aaaagagaat gcaacaaatc
    27001 aatgtcgcat tcccttctga tatattttat tcagcactta ttttgaactt tctgaggtgc
    27061 tagctgatga acttgtggag ctggaagatt cttcttttga agagttgctt gtgctactgg
    27121 agctacttga gctgctagaa ctgtctgaag agtctttttc agaactagaa gacgctggat
    27181 ctggtccttt agagtaaatt acgttcacaa ccattccttt ggtaattgtc gttcctgccc
    27241 ctggtactgt acgaatcact ttatctttat ccaccgtgtt tgaatattcg gaagtctcat
    27301 gcccgatata ttctgcaccg acactagcta aataactttg tgcttcgctt tttgtatagc
    27361 cggagatatc attcaattta ggctctgttc cttggcttac gacaaaattg atgtaagttt
    27421 cttctgctac aacttcaccc gaggctggat cctgactgat gatcgaacct gatggtacag
    27481 tgtcgctata gtcataagta gcactaatat tattgatatt gaatccttgt gtaacaagcc
    27541 aattacgaac agtttcgtag ttttctccta catgactact caaagtcact gtttctggtc
    27601 ctttgctgac tgtgaaagta atcgtgtctg ttttaggatc gacttctgta tcttctgatg
    27661 gagattgcga aatgatcatt ccgctttcta cctctgaact gaattcctct tctttttgaa
    27721 tcaagtcagc agaaaaccct aattctttca aacttgcttc ggcatcttca taacttttac
    27781 cactgtaatc atctaatttg atttttttcg tgccgctact aatatacaga gtgacttcgc
    27841 gtttttgctt gactgtcgtt cccgctgccg ggtctgtctt cactacatta ccttcttcga
    27901 ttttgtcatc tgcaatttct tctgtttttg ttgctacttc taatttcgct tttgcgagtg
    27961 tttctctagc agaagcttca gaaagatcag aaacatcagg aacaacaact tctcctcgcc
    28021 cgccagaagc aaagaataca gctgttccga ttcctaaaac agcaagaata gctaatagga
    28081 tcagccacca ttttttctgt cctttctttt taggatcagg cagaacttct tcctcctctg
    28141 gcaaggtttc gttttctggc ttgtcttttg gcagaggcat agatttgaat gaatcaggca
    28201 ttggtgcatc ttccgtaagg ggtgtgataa cttttgtttc attatccatc acatgaggct
    28261 gccatttttc ttcgtttgca cgagctggtg ataatactgt agaaagatca tcagacattt
    28321 cctcggctga cttataacgg tctgcaggtt ctttagccgt tgcccgtaaa atcacgtttt
    28381 ccagtgcttg tggcacattt ggatcaagtg cttttataga tggaagatca tcttgaaaat
    28441 gttttaaagc aattgttaca gcagattcac catcaaacgg tacgctaccc gtcaacatct
    28501 cataaaggat gatccctaaa gcataaatat ctgactgctt cgttgccata cttcctctgg
    28561 cctgctcagg ggacagataa tgaacagatc ccagcatcgt attcgtctgc gtgatcgatg
    28621 tttcagataa agcgatagca atcccgaaat ccgtgatttt tacaacacct tcattatcga
    28681 tcagtacgtt ttggggtttc aaatctcggt ggatgatttg atggttatga gctaaagaaa
    28741 tagcagaaag gatctgctgc ataatattta ctgctgtttc ataaggaaca ggataatgtg
    28801 tttggatata tcgtttcaag tccattcctt tgacgtattc catcaccaga tactgcatgt
    28861 tgtcttcttc accaacatcg taaacactaa caatgtttgg atgaacgagt tctgttgctg
    28921 ctaaagcttc tctctgaaat cgtcgaatcg cagtctggtc attttgaaag tcaaatcgta
    28981 agactttaac tgctacatca cgatccaaga ttaaatcgtg cgccaaaaat acatttgcca
    29041 taccaccgct accgatattt ccagtgattt gataacggcc atttaatttt ttgcccaact
    29101 cgatcataca ctgtcctcct tatattcgat aagaagaacg gtgatattat ccgctccgcc
    29161 tgcttcattg gcacgatcaa tcagctcagt tactttatga gacaatgggc cttcttgatt
    29221 gatgatcgtt cctatttctt cttcagacag catgttcgtc aagccatctg aacataacag
    29281 cagtcgatct tttagttgcc aaagatgagt agaaacatct acttcgactg ttccaggcat
    29341 gccgacagaa cgtgttaaaa tatttttcct tggatggttc actgccattt cttcgctgat
    29401 ttctcctgat ttgactagtt cattgactaa agaatgatcc tccgtcaatt gcatgatttt
    29461 atcttcacga atcaaataag ctctgctatc cccaacattg gcaatcgtga accagtcatc
    29521 tgacagagcg gccgctacga ttgttgttcc cataccgtta tattccggct gttcttgtcc
    29581 acgctgataa atcagcatat tttcttcttg aatcgattga atgaaccatt gggcgatttt
    29641 ttcatcgtca acagcttctt gtttttccca agcttcgcct agattcgtta ccgccatctg
    29701 actggcaatg tctccggcac gatgaccacc catcccgtct gctaatacag caagcttgag
    29761 acctgaacgg ctggtaaata cactggcata gtcttgattt gtgttacgtc gtcttcctac
    29821 atctgattga tattcaatct gcattttttc acctcattac tttttgcgca aacaacaaat
    29881 gaaaaatcca tctgtgtaaa actgatgcgg atacaaagtc aacattttct cttcgattga
    29941 tgattgtagc ataggattgg tcaaaacatc aatcctctca aaatcagggt gtctttccaa
    30001 gaaagcagaa acaacgtctt ggttttcttc ttttaagatc gtacaagtgc tataagtcag
    30061 aataccggaa gactttaaag ttggtgcgca gctttctaaa atttccaatt ggatttttgg
    30121 taaattcgct aattcatccg gctgtttttt ataacgaata tctggttttc gccgaagcag
    30181 cccaagacct gaacaaggcg cgtctactaa aatccgatca aactgctctg cttcgaactg
    30241 tttactgact tctctagcat ccatcttttg agcgtagaca gcagaagaga cacctagacg
    30301 tgctgcatta tcttcaatga gtttgacttt gtgttcgtgt acatctaaag cgaccacttc
    30361 tccaccgcat gacggatcta aaaaagaagc aatatgcgtc gtcttacctc cgggtgctgc
    30421 acaggcatct aagacatgat gttcaggctc gatttgcata gacggcgcta caagcatcga
    30481 actttcatct tggatcgtca tgacgccatc gtggaacaat gagctagaag ctaaatgccc
    30541 ttttttcgct actataccgt atggactgac ttggcttttt tcagctgtga tctcttcttc
    30601 ttgcaaaaca gtgatggcct gatctctgga aagtcggcgt gtatctactc gggcactagc
    30661 atggctaggc tgatatagcg ataaaccgag cttttcagtt tcttcgtatc cgatttgatc
    30721 caccagtcgt ttcgtcatcc atagaggcag gctgattttg acagaaagtc tttcgatagg
    30781 atcagcaatg ccttgcagat ctggtgctcc ttgacgctga taattcctca agacaccatt
    30841 tacaaatttc cctgtcccag gatttccttt tacttttgcg atttcgaccg cctcgtttaa
    30901 aatagcatgt gcagggactt tatctaaaaa ttctaattgg tatacagaaa gataaagaag
    30961 cgtcttgacc cagtcatcca ctttttttgc tttagcgatc aatggtgcaa cgtaaaattc
    31021 caatagtcgc tgatggctaa tcgtaccata gatcaattct gtaaataaac ccacatcttt
    31081 ttgatttagc tcgctttgtt tgatcatctc attcaacaaa agattcgagt aggcgccacc
    31141 cttgttcact cgctctaatg tcagtaatgc catatatcgt actgattttt tcattttttt
    31201 agggattttt ttcattattt cacctgctga ccaatctgaa cttgctgacc gcttccgtta
    31261 aggaaatcat gtacggcctg ttttccttta ccagctggct gtaattcatt gatggctaag
    31321 acggtctgtt taccgcaagc aatccataga ttttttttgt ctttccggat gatcgtacca
    31381 ggtgctttgt ctgtagtttc tgccaatact tcaaccgata ataatttcca gcgtatgccc
    31441 tcataggtcg tgaatgcaat tggccaagga cgcattccgc gtacttggtt gtcaatttct
    31501 tctgcagttt tatgccaatc gattgcttct tgttcccgtg tgatgtttgg cgaaaaagtt
    31561 gcttctgact cgtcttgagg tactggtttt tgtccatcca aaatattggg caatgtttct
    31621 agcaatagct ttcttcctaa aaggctaagc ttttcaaaca tcgtccctac atcatcttgt
    31681 ttagtgattg gtatgctttc ttgcgcatag attccgcctg catccatttt tttgatcatt
    31741 tccatgatcg ttacaccagt ttctttctcc ccattcatga tggcataatg aacaggcgca
    31801 cctccccgat atttaggaag taaagaagca tggacattga ttgcccctaa ttttggtact
    31861 tgcaataact tttcaggcaa gaattggcca aatgccgcag tgacgatcac atcaggcacc
    31921 aattcaatga tttcttccat ttctggcgaa ccggagattt tctctggctg cagcacacgg
    31981 ataccgtgct tcagagccgc ttcttttacc ggtgtcggcg taattactcg tttccgccca
    32041 actggacggt cgggctgtgt taccactgcc tggatctcgt aacctgattc tatcagccct
    32101 tcaagaatcg gcacagaaaa agcaggtgtg cccataaata ctagcttagt catcaagatg
    32161 ttcctccata tattcttcaa gttcttcttc cgggatctgc tgtatcattt tctcaatgaa
    32221 caagataccg tctaagtgat cgatttcatg ctggaaggca cgggctaaat agccaaaagc
    32281 cgtcacttct atctcttctc cgtctcgatc ataataacgc acggtcactt catctgctcg
    32341 cttgaccgtt ccatagacat gcggaatact gagacaacct tctacgtcaa ggctttcacc
    32401 ttttgcttcg atgatttctg ggttaatcag ttcaaatttc tcgccttcat ctacttcgat
    32461 cacagcaatt ctcttgtttt gtcctacttg aggagcagcg atccctattc cgtcattcgc
    32521 aatcattgtc tcatataaat tgtctaataa agcgattgtc tcgtccgtga ttacatcgat
    32581 tggctgagca gttcttttta atttatcatt agggtggatc aatataggat agcgcataat
    32641 ttggcccttt cttaaataaa gtttaatggt tcgttgtcaa ttgccacaaa aagaccttgt
    32701 cgctgttctt tctggcttcc attcaaaatt tcatccaaaa catgaggaag attcggttca
    32761 tgtttatatt tgatgattag ttgataataa tatcggtttt tcactctcaa aatcatgctg
    32821 ggcgttggcc ctaatagcaa gctttgaggg cttaacactt cttttagctg attggcaatc
    32881 tgaaagattt tctttgccgc tacttgttct tcaggatggc tggcggttat tttcacagta
    32941 aagtaataag gaggatagcc tccacgatgc cgtacatgca tttcctgctg ataaaactgc
    33001 tcataatttt gttttttagc taattctatg gcgtaatgtt gaggattgaa tgtttggatc
    33061 acgacttcgc ctgctttttc cgctcgccct gctcgtcctg aaacttgggt aagcaattgg
    33121 aatgtatgct cactagaacg aaaatccggt aaattcaggg aggtatcagc attcaacacc
    33181 ccaactaatg tcacttctgg aaagtccaaa cctttagcaa tcatttgtgt ccctaataat
    33241 atatctgctt cttttgcacc gaatttttgt aaaatctgtt cgtgtgcccc ttttcttcga
    33301 gtggtatcta catccatacg caaaatccta gcttgcggat aaagttctcg gagttcctct
    33361 tctacttttt gagtacctgt tccatagtag cggattttat tgccgccaca attaggacag
    33421 cgattgggaa taggttcttc atgtccgcaa taatggcagc gcattgaacg tgtatccata
    33481 tgcagtgtaa gagagatatc gcaattagga catggaagaa caaatccaca gtccctacac
    33541 atcacaaaag acgaatatcc tcgacgattg agcagtagga cagtttgttc ttttttgtct
    33601 aatcgatttt gtattttttc ctgcaaatta gcagaaaaag tagaggtccg atgattctga
    33661 aactcttcac gcatatctac gatctcaata gctggaagct gtgcagctgc tttggctcgg
    33721 tggtttaact gcaataactg ataaacgcct ttttgagctc gtgctcttga ttctaaagag
    33781 ggcgtcgcgc ttcctagaac tattgggcaa tgatgatatt tactgcgcca aattgctaga
    33841 tctctcgcat gataacgcgg tgtctcatct tgtttgtaac ttgattcatg ttcttcatcg
    33901 ataatgatca gcccgatatt tttcagcggg gcaaaaatag cagatcgtgc gcctactact
    33961 acttgcgctt cttcccgctc aatcttgcgc cattcatcat atttttcacc ttgggaaagt
    34021 ccgctatgta agaccgcaac tgattctccg aagcgtcctt tgaatcgctc gaccatttgc
    34081 ggagtcaatg cgatttcagg cactaacata atagccgttt tattttctga caaaacatca
    34141 gcgattgcct gaagatatac ttctgttttc ccgctgccag tgattccctc cagcaaaaag
    34201 acctgatctt tttgctgctg acctgctgtg atgatttgtt cgacagcatt tttttgttct
    34261 gcattcagtt ccaatgccgt cgtttggtca aatactcggt ctttataagg gtcgcgataa
    34321 cgttctgatt ctataaactc cagccagccc ttttcttttc cttgattcaa gacagcagta
    34381 ctgattcctt tatttttgaa ataagcaatt ggttcttctt ggctcagttc agataaacac
    34441 aaaagaagtt ctttttgttt cttggcattt tgccgtaatt ttgcccattc ttcttcgatt
    34501 tgcttttcag ttaacaaaga acggaccaaa cgaatcgttt ttactttatt tcgtgtatgg
    34561 acttcatatt tgatatctac taattgctct tggcgccagc gaatcaattg agaaaggctg
    34621 ccttcttctt ctgcttcctt ccaggaaatc acttctcgct gatcaaacaa tacctgaact
    34681 tctggtgtgt ctgccaaagg atagatcaat ttatcatatt ctgctttcat cacactcgga
    34741 agcatcgttt gtagacaagt gattttgaat gcgtaagtaa tttttttcat ataatcagct
    34801 aatgaaagta attcttcatt gaccaccgga gcaaggtcta gcaaacgaat aactgccttc
    34861 aaagatggat tagctgattc ttctgatttt tggatagcca taacaaatcc ttggacatga
    34921 cgattgccat taccaaaagg gacttctact cgcatcccga cttgcaaagc tgtttccatt
    34981 tcagagggaa ccaaataagt gaatggctga tcggtctgca tagtaggtac gtccacaatg
    35041 acttcagctg ttaatgtcat ggttcgtttc ctcctttttt tgtcttttct tatcaagcga
    35101 caaaaagttc cttgcctatt ttactagaga tcgatggatt tgtcttatct tctgccaaat
    35161 cctaacaaaa aattaacagc agataagaaa aaaaagaact gtgtcaaaaa gttcagacac
    35221 agccttttct accagacaat aagtggcgaa tgtttgaaaa ggaaacagct gggtttgctt
    35281 ttctttttag atgttcgctc tatttttctt tattacattt tttgctcttc acggatacgt
    35341 gattccaatt cgtgttgttc acgctctttt tgtgcttgac gttcctcttc ttccatcttc
    35401 aaacgttcac gttttaattc aggatgtgga tcattgacta catttcctgc atcgatttct
    35461 tctaaagctt gaccgacgct ttttactgaa tcaaaagaat ctagagtagg ctgtgcactt
    35521 gcatccaatt catgcgcgcg tttacttgct aaaatcacta gagaatattt tgaatttaca
    35581 cgatctaata gtgagtcaat tgaaggtttt aacatcataa gactacatct cctttaacat
    35641 tttaatatat ttaccgatta cgcgatctac tcgaaaatgt tcactggcaa taatgttttt
    35701 gattcgttca actgctagcg gcacttcatc atttacaaca gcatagtcgt aaagagccat
    35761 catttcgatt tcttcttttg caactttcat tcgttcttcg attacttcat ctgcatctgt
    35821 tcctcggcct acgatccgtg attttaattc cgctaaatct ggcggagtca aaaagatgaa
    35881 tacaccatcg ggcacttttt ctttgacttg ttttgctcct tggacttcaa tctccaagaa
    35941 aacatctttt ccttcatcca atgtcttgtt gatatatgac aaaggagttc cataataatt
    36001 cccgacatat tctgcgtatt ccagcatctc tccagcttcg atcattgatt caaactcttc
    36061 tttgcttcgg aagaaataat caacgccttc gacctcccct tcacgcattt tacgggtagt
    36121 cattgagatc gaatactgaa aatcattatc ctcactctca aaaattgctt ttcgtactgt
    36181 gccttttcct acaccggatg gaccagacaa cacgattaat aatccacgct ctgacatgac
    36241 gacgtccttt caactagtct ttttgtactt atcgtttatt ttgcactttt tttctgcaac
    36301 tttcaaggtt atctttaatt atagctagaa attggtaaaa aagctaccct gtaccaagag
    36361 gtcgtaaaaa agctgtcctg catcaagtaa taagaacagc caaacataaa cagcttctca
    36421 tgtttttcgt ttggttgggg ttattatcgc agatgcaagc ttttgaacgc cgtgtataag
    36481 aggtcgtgaa gaagccgatt agtttcgagt atgaagaaaa attttgtgaa aatagtttct
    36541 cacatttttg cataaatttg gcttaatac
//
LOCUS       DAZABD010000021        36550 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000021 DAZABD010000000
VERSION     DAZABD010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36550
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.021"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            23..694
                     /locus_tag="ACHALG_001146"
     CDS             23..694
                     /locus_tag="ACHALG_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFN2586570.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            753..1193
                     /locus_tag="ACHALG_001147"
     CDS             753..1193
                     /locus_tag="ACHALG_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2586571.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1205..1873
                     /locus_tag="ACHALG_001148"
     CDS             1205..1873
                     /locus_tag="ACHALG_001148"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFN2586572.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1937..2560
                     /locus_tag="ACHALG_001149"
     CDS             1937..2560
                     /locus_tag="ACHALG_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFN2586573.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2616..3002
                     /locus_tag="ACHALG_001150"
     CDS             2616..3002
                     /locus_tag="ACHALG_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HFN2586574.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3215..4606
                     /locus_tag="ACHALG_001151"
     CDS             3215..4606
                     /locus_tag="ACHALG_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HFN2586575.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            5217..5585
                     /locus_tag="ACHALG_001152"
     CDS             5217..5585
                     /locus_tag="ACHALG_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFN2586576.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            5765..6706
                     /locus_tag="ACHALG_001153"
     CDS             5765..6706
                     /locus_tag="ACHALG_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFN2586577.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            6775..7506
                     /locus_tag="ACHALG_001154"
     CDS             6775..7506
                     /locus_tag="ACHALG_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFN2586578.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            7650..9830
                     /locus_tag="ACHALG_001155"
     CDS             7650..9830
                     /locus_tag="ACHALG_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFN2586579.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            9850..10332
                     /locus_tag="ACHALG_001156"
     CDS             9850..10332
                     /locus_tag="ACHALG_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HFN2586580.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            10348..11109
                     /locus_tag="ACHALG_001157"
     CDS             10348..11109
                     /locus_tag="ACHALG_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFN2586581.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            11240..12574
                     /gene="murC"
                     /locus_tag="ACHALG_001158"
     CDS             11240..12574
                     /gene="murC"
                     /locus_tag="ACHALG_001158"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFN2586582.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            12881..13366
                     /locus_tag="ACHALG_001159"
     CDS             12881..13366
                     /locus_tag="ACHALG_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HFN2586583.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            13359..14042
                     /locus_tag="ACHALG_001160"
     CDS             13359..14042
                     /locus_tag="ACHALG_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFN2586584.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            14168..16813
                     /gene="polA"
                     /locus_tag="ACHALG_001161"
     CDS             14168..16813
                     /gene="polA"
                     /locus_tag="ACHALG_001161"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFN2586585.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            16858..17694
                     /gene="mutM"
                     /locus_tag="ACHALG_001162"
     CDS             16858..17694
                     /gene="mutM"
                     /locus_tag="ACHALG_001162"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFN2586586.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            17658..18287
                     /gene="coaE"
                     /locus_tag="ACHALG_001163"
     CDS             17658..18287
                     /gene="coaE"
                     /locus_tag="ACHALG_001163"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFN2586587.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEILVGQIENWLFANRFL"
     gene            18339..18671
                     /locus_tag="ACHALG_001164"
     CDS             18339..18671
                     /locus_tag="ACHALG_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFN2586588.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            18983..19474
                     /gene="nrdR"
                     /locus_tag="ACHALG_001165"
     CDS             18983..19474
                     /gene="nrdR"
                     /locus_tag="ACHALG_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFN2586589.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            19497..20870
                     /locus_tag="ACHALG_001166"
     CDS             19497..20870
                     /locus_tag="ACHALG_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFN2586590.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            20870..21796
                     /gene="dnaI"
                     /locus_tag="ACHALG_001167"
     CDS             20870..21796
                     /gene="dnaI"
                     /locus_tag="ACHALG_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HFN2586591.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            21925..22365
                     /locus_tag="ACHALG_001168"
     CDS             21925..22365
                     /locus_tag="ACHALG_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFN2586592.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            22509..23210
                     /locus_tag="ACHALG_001169"
     CDS             22509..23210
                     /locus_tag="ACHALG_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HFN2586593.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            23207..24328
                     /locus_tag="ACHALG_001170"
     CDS             23207..24328
                     /locus_tag="ACHALG_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFN2586594.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            24343..25575
                     /gene="pepT"
                     /locus_tag="ACHALG_001171"
     CDS             24343..25575
                     /gene="pepT"
                     /locus_tag="ACHALG_001171"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFN2586595.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            25707..26615
                     /locus_tag="ACHALG_001172"
     CDS             25707..26615
                     /locus_tag="ACHALG_001172"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFN2586596.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            26650..26922
                     /locus_tag="ACHALG_001173"
     CDS             26650..26922
                     /locus_tag="ACHALG_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFN2586597.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            26933..27979
                     /locus_tag="ACHALG_001174"
     CDS             26933..27979
                     /locus_tag="ACHALG_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HFN2586598.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            28230..30839
                     /gene="clpB"
                     /locus_tag="ACHALG_001175"
     CDS             28230..30839
                     /gene="clpB"
                     /locus_tag="ACHALG_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFN2586599.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(30990..31676)
                     /locus_tag="ACHALG_001176"
     CDS             complement(30990..31676)
                     /locus_tag="ACHALG_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2586600.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(31654..32148)
                     /locus_tag="ACHALG_001177"
     CDS             complement(31654..32148)
                     /locus_tag="ACHALG_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFN2586601.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(32168..33388)
                     /locus_tag="ACHALG_001178"
     CDS             complement(32168..33388)
                     /locus_tag="ACHALG_001178"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN2586602.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            33535..35370
                     /gene="lepA"
                     /locus_tag="ACHALG_001179"
     CDS             33535..35370
                     /gene="lepA"
                     /locus_tag="ACHALG_001179"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFN2586603.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
     gene            complement(35464..>36550)
                     /locus_tag="ACHALG_001180"
     CDS             complement(35464..>36550)
                     /locus_tag="ACHALG_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN2586604.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT        12163 a         6465 c         7816 g        10106 t
ORIGIN      
        1 tgatgaaaaa agggggagaa aaatgaattt tttagttgtt ttaaaagata ctctgattga
       61 gcgatctaca tttctatatg ttcaattgca ggaaaataaa acattgctgc atttttcacc
      121 agagttccat ctagaggggc taacattagg agaaatctat caagcagaag ggctgtcggc
      181 tgttttgcac ttttttaagg tagaattgga attaccagta aaagattata tcgagctttc
      241 tcttgaatct tgggatcgag cagtggttga actgtttcca gaaggtttaa tgatcgatac
      301 aaacgatggg aagatccatc tgactgcagc agaactgcaa aaacaaatgc gttatcaaat
      361 agatgacgaa aattcttttt ctatttttag acgtcagcaa aagattctaa aaagcttttt
      421 aaaagtgatc agtaaaaaac ggaatctgat caaaacgaca cagctgttga aaaaatatcc
      481 aggtctgctt cacacgtcta ttcaatttcc tcaattgatt acgatgatcc atcgttttat
      541 acgtatgcaa cagctaccat ctaagaagct gtttcttcca ttgacggaca catttcgagt
      601 agtcaacaga gaagataaaa agaaagtgat tgtgattgac tttaccagaa atcggaatgt
      661 ccttcaccaa gttgcaatgg aaaaggccaa ttaaggcttt ttctttttgg atttttttgt
      721 cataataaat attagatgat ggaggagata atatgatgca aacatggtta gaaaggctaa
      781 tacgatttca gcagaactat cacaaactta tgcagggaag atatgcgaaa tttgatcagt
      841 tgaatcgcgg tctattgatc gctgcgcttg tctttagttt gctaagtcgt tggttgccct
      901 atagaatcgg acaattgttg ttcatcgtct ttttcggatg gatgatattt cgtttcctat
      961 caaaaaaaat ctaccccaga ctaaatgaga atcaacgtta tttgaaatat cttgatagag
     1021 tcaaagaaaa atatcatcag ataaaaaaca aaaagcaagt caaaatggat cgaaaaagtt
     1081 atactttttt tgagtgcccg aattgtcatc aaaagcagcg tgcacctaaa ggaaaaggac
     1141 gaatacgtgt gacatgcaaa acatgtggta taaaattcga aacgaatgtt taagggagaa
     1201 aagaatgaaa ttagtattat tacgtcatgg cgaaagcgaa gcaaattttg aaaattactg
     1261 gacaggatgg ctagatgtcg ccttaacaga aaaaggaaaa gaacaagcag cagttgccgg
     1321 tcaaaaaatg cttgaagccg gacttcaatt cgatgccgtt tatacctctg ttttgaaacg
     1381 tgcgattctt accggacaag tcgcactaga agaaatgggt cagttatggc ttcctataat
     1441 aaaaacatgg cgtctaaatg aacggcatta tggggatctt gtgggtaaaa acaaagataa
     1501 gatgaaagaa atatacggaa aagatcaagt aaaaacatgg cgcagaggat atacggaagt
     1561 tccgccgtta accgcagaaa atagatttga ccgcagatat gatcacttgg atccgcattt
     1621 gatcccatac ggagaaagtc tggaaatgac agtgaatcgg atcattccct tatggcaaga
     1681 tcatctagca ccaaaactaa aagatcggca agatttacta gttgtgggtc atggtaatag
     1741 catccgagca ctgacaaaat atttagaaga tgttcctgaa gatcaaatgg atacgatcga
     1801 tatcccaaat gcacaaccaa tccagtatac attagcagat gatttgacaa ttctggacaa
     1861 aaaaatactt taaagaacac ctttatctaa aagtgagaga tagaaaaaag aagcaaagga
     1921 atggaacaaa aggaacatga aaaattatat atgggacttt gatggtacat tgtttgatac
     1981 gtatcccgca atggtagatg gtgcatggca agcactaaaa gattttggta tcagtatgga
     2041 caaaaaagaa atctacttca aaatgaaaaa gtattctact agttatttga tcaatgaatc
     2101 aaacttgaat gccagagagt ttaatgaatt atttcaccga tatgaaaaag aaagtacgga
     2161 agtttcccgt ccatttccag aaacgaaaca agtactggaa atgctaaaag acaacggcgg
     2221 tcgtcatttc atcctgaccc accgtttgac agaatcaaca tggggcctgt taaaagaaca
     2281 tcgattggca catctgattg aggaagtagt agggatcgat caggactttc ctcgtaaacc
     2341 tgatccagca tctttaaatt atttgatcga tacttttcat ttagaaagaa cagacacgat
     2401 gatgatcggc gatcgtcgtt tagatatcga agcagggaaa aatgcaggag tcgctacttg
     2461 tctttacgac atcgatcatt ttctgggtga gatcccggca gattatgtgg taggaaatct
     2521 gaatgaaatc cttaccttgc agagcatcat taaaaagtag tatccccttt caaaggagat
     2581 atgttataat tttcataaag gagtgataaa attatatgga aatcagaaga caaaaagaag
     2641 ttgtagaagc atttcattat gatatgagat tgccgaacca agaagtagaa acagatttga
     2701 aagtaggctt ctcgccttta aaagaaacga tcgaaaacta tccgaaagaa catagcatcg
     2761 taggagcacg cttagagttc cgtttagtgt ttgatacata tgttttatct ggaagtgtga
     2821 gtcagatcaa tcatatcctc aatcggaaaa ttgaaggtca aaatgatgtc acacaagaag
     2881 aagtggacga gctagtagca ccgttattcg atatcgtacg ccgaatgtct tatgaggtga
     2941 cagagatcgc cttagatcag cctggggtaa aattgaattt tcaatcagaa aaacaagaat
     3001 agacagtaaa aacatacctg cgatagatta aagctatcac aggtatgttt ttttattact
     3061 aattgtaagc aaaaagaatg tcatttcgat aaaaatcgtc taaaatataa aagtcactga
     3121 aaaaaatttt ttcaggagga tggttgaaga atcattctcg ggaaccttta cttccttcga
     3181 tggacttgtc attaataata tgaggaggag aaaaatgaca acactcatca cagttttagt
     3241 ggttctagtt tttgcggcat tattatatgt gttctatcgt atgcaaaagc gccatacaaa
     3301 attttcaaca cgcgtcttta cagcattagg agttggtatc gtctttggcg gtctgcttca
     3361 attgatcttt ggtgtagacg acaaagtcgt tactcaatca atggaatgga tcgggattgt
     3421 aggtcaagga tatgtatcat tcttgcaaat gctagttatg cctttagtgt ttatttcgat
     3481 cattggtgca ttcacaaaaa tgaaagtttc tgaaaagcta gggaaaatta gtgcgactgt
     3541 attgacttct ttacttggaa caacagctat tgcagcattg atcggaattg gttcaacaat
     3601 gctgttcgga ctccaaggag ctaaattcac acaaggagct gcagaaacag cacgaatctc
     3661 tgaactggca acacgtcagg aaacagttga aaacttatcg attccacagc agattttatc
     3721 ctttatccct aaaaacgttt ttgctgattt agcaggtagt cgtgcaacaa gtacgattgg
     3781 agtcgttatt ttcgcggcat ttgtcggaat cgcttatcta ggcgtgaaaa gaaaagcgcc
     3841 aaaagaagca gcattctttt cagatttagt cgacagtctg tataaaatca ttatgcggat
     3901 cgtgacgctt gttttgcgtt tgacacctta tggagtattt gctttgatga caaacgtttt
     3961 ggcaacaagt gattttgcag ctatcgtcaa tttaggtaaa tttgtcgttg cttcttacgt
     4021 agcattgctt gctgttttga ttgtccactc actgattttg attggtgcaa aagtcaatcc
     4081 aatcaactat ttcaaaaaag cctttccggt attgagcttt gcgtttactt cacgttcaag
     4141 tgctggagct ttgccattga atatcgagac tcagacaaaa gctttaggag tagatgatgc
     4201 aacagctaat ttctcagcaa gttttggttt gtctattgga caaaacggct gtgctggcgt
     4261 ttatccagct atgttggcaa cgatcgtcgc accagcagct ggagtgaatg tctttagttt
     4321 ggaatttatt ttgatgcttg tggcaattgt aacaatcagt tcttttggtg tagctggagt
     4381 cggtgggggc gcaacatttg cttcactgat cgttttaggg gcaatgaact tgccagtagc
     4441 aatcgttggg ttagttatct ctgttgaacc attaatcgat atggcacgta cagcagtaaa
     4501 cgtcagcggt agcatggtag ctggtatcgt gacaagtgca cgcatcaaag atttagatcg
     4561 cgatgtcatc aatgatgatt ctaaagtgat cgatgtgaat gcatagtcaa aaagaggttg
     4621 tgatagaagt ggtcagcttt aagaaataag aagaagtttc ctaaaaatag tttctcaaat
     4681 ttttgtgaaa tttcggctta tttccgagaa gctacttctg aaacaccgtg aataagaggt
     4741 tgtgaaaaag ctgccctgca tcaagtaata agaacagcca aacaaaaata gttcctcata
     4801 ttttacgttt ggttgagctt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
     4861 aggtcgtgag cctgccgttt cactctgagt cacaaagagc aatttcaaaa aatagcttct
     4921 catgtttgtt gaatattgtg tcttgtgacc gaggagttgt caggagaaca ccgtttacca
     4981 gaggttgtga cttgttttta gtcacgacct ctttttttat ttattccata taaatacaca
     5041 ttatttattc cgaaaagtga aagcgcaaat aacaaaaaca accaacttat ttattattaa
     5101 agaaaagcgt atttaagtag gacgattctg ttgagagctt tcgtagattt tcccattttc
     5161 ctatggtaaa ctgacaaagt tgagaatgtt agaacagatc gtttaggagg aaacagatgt
     5221 ttacgaatga aataaaaatc atgctttatg tggacgatgt agaaaaaaat gcaacgtttt
     5281 ggagagcagt tggctttgcg gaaaaagatc gtcaagaaat ggatgggacg cttataattg
     5341 aagtaggagt tgaggacagt caaacctcat ttctgttata tgatcgagca tttgttgagt
     5401 cccattcacc tggcacagct actagtgccc catctttgat gttttacagt aaagatatct
     5461 ttggactata taaaaaaatg cagacgcaat cagtcacatt aggtgatatg gtacagcttg
     5521 gagaagaata tgtctttaac tttgctgatc ccgatggcaa ctactttgcg gttacaggta
     5581 aataatagaa ataaagactt tgtttttctg tgcgaaacag gaaagcgaag tctttttaat
     5641 tcaaataata atattatgta aacaaaaaac tttccatgcg taattataca caagcaaata
     5701 aatcgtcaaa tgcgaacgcc ttctttatac tgtttaggaa cgaaaagaag gaaggaagaa
     5761 atacatggat tatcaagtac tgctttatta caattataca aaaatcgaag aaccagagga
     5821 atttgccaaa aatcatttag ccttttgcaa atctttggat cttaaaggtc gtattctagt
     5881 agctgaagaa ggaataaatg gtactgtgtc tggaaccgtt gaaaacacga ctgcctatat
     5941 gaaagcaatg aaagctgatc ctcgatttgc tgatacgatg ttcaagatcg atccaagcga
     6001 aggacatgcc tttaagaaga tgtttgtaag ataccgtcca gagcttgtaa gtttaaagct
     6061 agaagaggac attgatccaa accaagtgac aggtgcctat ctttcaccgc aagaatttaa
     6121 agaagctatt ttagatgaac agacagtggt gattgatgca cggaatgatt atgaatatga
     6181 cttgggacat tttcgagggg ctgtacgccc agatatacgc agttttcgag agcttccaga
     6241 ctggatccgc gagcacaaag aagaatttat ggaaaaacgg gtagtgacct actgtactgg
     6301 tggcattcgt tgcgagaagt tttctggttg gcttgtccgt gaaggattca aagatgtcgg
     6361 acagctgcat ggcggtattg ctacttatgg taaggatcct gaagtccaag gagaattatg
     6421 ggacggcaag atgtatgtgt ttgatgaacg gatcgctgtc gatatcaacc aagtagataa
     6481 acaaatcgtc ggtcgcgact ggtttgacgg aacaccatgt gaacgatata tcaattgtag
     6541 caatcctgag tgcaatcgtc agattcttgc ttccattgaa aatgaagaaa aacatttagg
     6601 agcatgtagc atagagtgtg cgaaacatcc gcgcaatcgc tatgttcaag accatggaat
     6661 ctctccagaa gaagcagaac tgcggtttac agctttaatt aaatagaaaa caatagtttt
     6721 gattgtcgac catttccaat atgcgctata ctagcgttga ggatggtgat ccacatgaaa
     6781 tcattgattt atgctattgg ggatgtccat ggcaaacatg agctttttga aaaacttatt
     6841 cactattatg atccaaagat ccatcagctt gttttattag gggatctcaa tgatcgaggt
     6901 cctcgtacaa aagaatgttt tttattagga aagcaattag tcgaagaaac aggagccatt
     6961 tacctccggg gaaaccatga agaatatttt ttgcaatttc tgcaatcacc ggaggactgg
     7021 tatgcgcctt atatgagaaa tggtggaaaa gaaacgtttg aaagcctgat tcacccaggg
     7081 gcatcggagg aatactcccc aactgaaatc tcgatgatga tccaatcgag atattcagaa
     7141 ctgattaaat ttttagtcca gcgccccttg tattatgaat gggaaaatta tctttttgtc
     7201 catgccggtg tggatttgac aaagaaagac tggcgtaaga caagtctaag agactttatt
     7261 tggataaggg aaccatttca tgaagggaaa aacaatacag gtaagacaat tgtatttgga
     7321 catacgatta caccaatgct ccatggagat atgcagacaa cagacctctg gattcaggat
     7381 cataaaatcg gaatggacgg tggggctgta ttcggagggt cattgcatgg ggttgttttt
     7441 gacaaggaag gtattcttca agatattgaa tatcagaaca acgaaggacc ttggcatgga
     7501 atctgactaa tggaaagaat gaaggagctg tgactttgtt gcggctcttt ttatttttat
     7561 agagaatatt gtttctttca gtagtttaac ccaggataaa aagtatgata gaataaagaa
     7621 gcaattttta gcagaaaaga ggcattttaa tgacggaaaa tttgaaccag acgatcattc
     7681 agcttgtaca aaaagaactt tcagactatc gtcctaagca gttgactact gtacttaatt
     7741 tgttaaatga aggaaacact gttcctttta tcgcgcgtta ccgaaaagaa atgactggaa
     7801 gtcttgatga agtccagatt cgtgaaatcg aagagcggta tgcctatctt gaaaacttag
     7861 aaaaacgtaa aactgaagtg attcggctaa tcgatgaaca agggaaactg actcccgagt
     7921 tagaagcaga aatcacccaa gccgtgaaaa tgcaacaagt ggaagatttg tatcgtccat
     7981 acaaacaaaa acgacggaca aaagcaacga ttgctaaaga aaaaggactt gaaccactcg
     8041 cgaagtggct gatgcagctt acagatggag aggtgcaatc tgaagcagaa aaatatattg
     8101 acaaagaaaa agaagtctct tctgccgaag aagcgttgca cggtgctcat gagattattg
     8161 ctgaacaagt cagcgataat gcaaaatttc ggacttggat tcggtcttat acgtataaca
     8221 aaggtatgta tgtcagcaat gtcaaggatg agcaagcaga tgaaaaaggc gtatatgaaa
     8281 tgtattatga ctttgcagaa cccgtccata aaatggtttc tcatcgaata ttagctacta
     8341 atcgtggaga aaaagaagat attctgaaag tattctttca agtagatgaa gcagcaattt
     8401 tagcttactt ggatcgccaa atagtcaaaa atcctgcctc tccttcttct tcattcgtca
     8461 gagaagccta tcaggatagt tataaacgct ttattcagcc agcaatcgag cgagaactgc
     8521 gcaatgaatt gactgaaaag gcagatgagc aagcaatcgc tattttcggc gaaaacctcc
     8581 gtaatctttt gttgcaaccg ccactaaaag gtaaagttgt cctgggattt gatccggctt
     8641 atcgtactgg atgtaaacta gcagtcgtag atgcgacagg gaaagtttta gcaatcgaag
     8701 ttatttatcc tcataaacct gcagcgcaag caaaaagaga agctgctgga ccagctttta
     8761 tccaattaat taataaatat caagtagata tggtagctat cggaaatggt acagccagtc
     8821 gggaatctga actttttgtg gctgagcagt tgaaatccgc cgaccataaa acctattacg
     8881 ctatcgtcaa cgaagcaggc gcttctgttt actccgctag cgaaattgca cgaaaagaat
     8941 ttccgcatct acaagttgaa gaaagaagtg ctgtttctat tgcccgtcgc ctgcaagatc
     9001 ctttagctga attagtcaaa atcgatccta aagctgtcgg agtaggccag taccaacatg
     9061 acgtttctca aaaacgtttg gctgaacaat tggattttgt tgtggaaaca gcggttaacc
     9121 aagtaggtgt ggatgtgaat acagcaagcc cgcagctctt gcagcatata tcaggattga
     9181 ataaaacaac tgcccaaaac attgtcattt atcgtgaaga aaacggagaa ttcaccgcac
     9241 gaactcaatt gaaaaaagtg ccacgcttag gaccaaaagc atatgaacaa gcaatcggat
     9301 tcttgcgagt ccctggtggg aaaaacattt tggacaacac aggtatccat cccgaaagtt
     9361 attctattgc aaaagaaata ttaacttcag tccaactttc agaaaaagag cttggtacag
     9421 aagaagcagt agaaaaattg acacgattat ccgtagagaa actggctgaa agcctatcag
     9481 tcggagaaga aacgttagcc gatattttag ctgggctgac acaacctgga cgagatatgc
     9541 gtgatgagat gccagcgccc ttattacgta cagatgtatt gagtatggaa gacttgaaac
     9601 cagggatgga attgactgga acggtccgta atgtcattga cttcggcgca tttgttgata
     9661 tcggtgtgaa gcaagacgga cttgtccata tatcgaaact cagcaaaaaa ttcgtcaagc
     9721 atcctacaga tgtcgtgtca gtaggagata tcgtaacagt ttggatagaa caagtcgata
     9781 cgaaaaaagg cagaatcagt ttgacgatgc tttcccctta tgaagagtaa caatgaggag
     9841 taacaaaaat tgacacaaga agaactgcag cagttagtag aagagatatc attaaaaagc
     9901 ttccagcttc ctttcaagca ccaagcaatg ttcaatccac gcttacgtac aactggcggg
     9961 cgctatcatt taaacgatca tcatttagat tttaatgcga agctatttga agagtcagac
    10021 gaagagacga ttgcaggaat catcaaacat gaactttgcc attatcatct tcatctagct
    10081 ggaagagggt atcagcataa agatatggat ttcaagtatc ttttgaaaaa aacaggaggg
    10141 ttgcgctatg caccagcctt gacgaagaaa cagataaaga tggagtatta tcgatgccaa
    10201 gattgttctg ccacgatctg ccgcaagaga agaatcgatg tacgcaaata tcgttgtgga
    10261 agatgtcatg gagaaatcaa atggattgag acaaaaagag aagactttaa tgaagcacaa
    10321 aaaatcagat gaaaggatag aaggcctttg gaaaaaagtg agcagaggaa tctaaaagac
    10381 tggaatcagc tattaaattt tttagccgca cctgaagagg gaaattttga ggaactgacg
    10441 gcacctacac ttattttagc tggaaactgt cttcctatct tagctgataa agcagcacaa
    10501 atgtatttaa atggccaagt agatcaattg tttttggtcg gaggggtggg gcatgcgact
    10561 agaatccttt atgagaattt tgaaaagcaa ggatttcatt ttgaagatgg catgagtgaa
    10621 agtgaaatct gtcgtcagta tctaaaagaa gtctatgatt tgcccgataa ggcttttctg
    10681 atagaatcga agtctacaaa ttctggagag aacgccactt tttcactgga gattcttcat
    10741 tccttaggag ctgtaccaga aaaaatctta ttgatgaatg atccgacact gcaacgcagg
    10801 actcgagcaa cttttgagaa agtatggcaa aatgaacaaa ctattttcgc gaatgctgtg
    10861 ccatttgtgc cggaaatcct tcacttttct gaagagatca tctttacagc aaaagaactg
    10921 aatcatcaat ggcctaagga atatttccat gcgctagtat taggagaaat ggaacgtctc
    10981 catgacgatg agaacggcta tggtccgaaa ggaaaagatt ttattcctca cattgatata
    11041 tcagaggaag tttggtcttc ttacaccaga attaaagagt caataaaaac ggatttttct
    11101 cgtacttgac cattttagca agcgaatcct cctttccaaa taaatttggt tgaaattttc
    11161 aatcgtttat aagataatga ggagcgtgtt gaatgtaata gctaaaagaa tggacgctag
    11221 ataagagaag aggattcaaa tggaaaatca aaataaattg tatcattttg ttggtatcaa
    11281 aggttcagga atgagctcct tggctcttgt tttacatgaa caaggtctaa atgtccaagg
    11341 atcagacatt gaaaaatatt tctttacaca aagagattta gaaaaagcaa atatcacgat
    11401 tctgccattt aacgcagata atgtaaaacc agggatgacg atcattgcag gaaatgcatt
    11461 tcctgattca catgaagaaa tccagcgagc aaaagaatta ggtttggaag tgattcgcta
    11521 tcacgacttt attggtcatt ttatccaaaa ttatacgagt atcgctgtaa caggctctca
    11581 cggaaaaaca agtacgactg gactgctctc tcatgtattg agtggtgtcc gtccaacaag
    11641 ttatctaatt ggagatggaa caggacatgg tgatccgcaa gcggaattct tttcatttga
    11701 ggcctgtgaa tatcgccgtc atttccttgc ctattcacca gactatgcaa tcatgaccaa
    11761 tatcgatttt gatcatccgg attattatac aagtatcgac gatgtattta cagctttcca
    11821 aacaatggct ggacaagtga aaaaagctat ttttgcgtat ggtgacgatg cttatttacg
    11881 caaattaaaa gcaaatgtgc cgatttatta ttatggtgtc acagaaaacg atgatatcca
    11941 agcacgaaac atcgaacgca caacttctgg ttctgctttt gatgtctatc atggggatga
    12001 atttgttggg cacttcactg taccagcatt cggtaaacac aatatcttaa atgctttagg
    12061 tgttattgca gtagcttatt tcgaaaaact ggatctgaaa gaagttgcag aagaaatgct
    12121 gactttccca ggagtaaaac gtcgattcag tgaaaagatc gtagcagaca tgaccgtagt
    12181 cgatgattat gcgcatcacc ctgcagagat caaagcaacg attgatggcg cacgccaaaa
    12241 atatccagat aaagaaataa ttgcagtttt ccaaccccat acgtttacgc gtacgattgc
    12301 attgatggat gaatttgcag aagcacttga tttagctgat aaagtttact tatgcgatat
    12361 ttttggttcg gctcgggaag aacaaggaaa cgttaagatt gaagaccttg gagcgaaaat
    12421 caaaaaaggt ggagaagtca tcaaagaaaa taatgtatct ccattgttag attatcatga
    12481 tgcagtagta atcttcatgg gtgctggaga tgttcaaaaa tttgaacaag cgtatgaaaa
    12541 acttttaagc agcacgacga aaaatgtact gtaatacaat tactgcagtg tccttataaa
    12601 aatagaaaga gggggagtgg caaaaatctt gccaagcccc cttttttgca acttatgata
    12661 aacggtgttc aaaagcttgc atctgcggta ataagcccaa tcaaatcaaa aacatgagaa
    12721 gctgtttttt cttggctgtt cttattattt gatgcaggac agctttttca cgacctctgt
    12781 aaactatagg aatctgttaa atcaagggag cagaacttgc cttttacgct acttttggta
    12841 caataatgta tgattaaaaa taaactggag ggtttctatc ttgaacatag gtaagccgag
    12901 aaaactagga aaaacaataa atgaaatcga ggaaggcgac tcgttatcct taacagaatc
    12961 tattgaagat aaccagcttt tgctgtattt gggattgacg aatgatgcaa atcctttgta
    13021 tatccaacat gactttgcac aaaaaacaga atatcaacag ccaatcgttc catcagtgat
    13081 gctgatgggg atcatcacaa gtgcaatttc aaagcatctt cctggaccag gatcacatgt
    13141 tgtcaatttt tctgtcaatt ttattgaacc ggttttccat tatgaaacac tgacttttca
    13201 gtttgaggtc ataaaagtag ataagatgaa ggatgtggtg acgatttctg tagaagcgga
    13261 gaatacgaac gaagatcgtg tgttggatgc tgtcgttatg gtccaaccgc cacaagttcc
    13321 tgaattggaa atagcaatcg aaagtgaagg tgaattgaat gaataatcca agtacatcaa
    13381 tgtcaggtat caatcgtttt tatgcaaaag tttatggatt tttaggtatg ggacttgccc
    13441 ttagtgcagt cacttcttac ttggctcttt gggtatttcc tttgcaatta gcaactttta
    13501 tcaataattt tccactaggc tttgtgggtc tgtggatcgt tgaactgatt ttagtaatgg
    13561 ttctaggcgt aaaagcacaa aagaatccga gcttagcaat cggtggattt attgcctact
    13621 ctcttttgaa tggcataaca ttagcggtta ctcttgcata ctacgatatt ggaacagtaa
    13681 cgcaagcttt cttgacagct actttgatgt tcatggggat gtctgttgtc ggagtaacaa
    13741 caaaaagaga tcttagcggg gttggaagag caggacaggc agctttaatc ggagtgatca
    13801 ttgcactagt cttgaacatc ttcattttga gaagtagcgt agttgagctg ttcatctccc
    13861 tactgttagt cgtcatcttt gcagggatta cagcttatga taaccaaagc atccgtaata
    13921 tctatgtcca gacaaacgga acagcacata gtggaattgc tgtattcatg gcattgaagc
    13981 tgtatttgga ctttatcaac ttgttcttgg cgtttctgag aatctttgga cggaacaact
    14041 aaaaaagaaa aaaattcatc agttggggct ataccgaatt atttggtatg gtcccgcttt
    14101 ttcttttcgc attccgcagg attctttgtt agaatgaata agaactttac agtaaggagt
    14161 taagaagatg acaaaaaata aattattact cgttgatggg aacagtgtgg cttttcgcgc
    14221 attctttgcg cttcacaatt ctcttgaacg tttcaaaaat agaaacggtc ttcatacaaa
    14281 tgcaatctat gcattcaata cgatgtttga aaatgttatg caaaaagagc aaccgactca
    14341 tgttctggtt gcgttcgatg caggaaaaac gacttttcgg acagaaatgt atgcggagta
    14401 caaaggtgga agatccaaaa cacctggcga attcaaagaa caaatgccat atatacgtga
    14461 tttgttgaca ggactagggg tacagtatta cgaattgccc aattatgaag cagatgatat
    14521 tatcggtact ttggcagaaa aagtagacaa agatcaattt gatgtcgtcg tgctttcagg
    14581 tgatcgggat ttgactcaat tagctagtaa agaagtaaaa gtggatatca ctgtaaaagg
    14641 tgtcagcgat atcgaaagct atacaccaga acatgtagcg gaaaaatatg atgggttgac
    14701 gccaaaacag attattgaca tgaagggact agccggagat gcctctgaca atatccccgg
    14761 tgtgactaaa atcggtgaaa aaacagctat caagctattg aaacaatatg gctcagtcga
    14821 aggaatttat gaacatatcg atgaaatgaa gcaaagcaaa atgaaagaaa atctgataaa
    14881 tgacaaagag caggctttct tatcgaaaaa attggcgacc atcaatactt cagcgcctgt
    14941 ggatgtctct atcgactcat tgaagtatga agggaaaaat ctcgataaac tcgttccgtt
    15001 ttataaagaa atggatttca atcagttcct ttctaaattg aatatcgtag aagaagaagt
    15061 aaaaatggat gatattttgt ttgaagtcgt tcatgaattc aaagaggaaa tgtttactac
    15121 ggatatggcg ttgtacgtgg aaatgatggg cgacaattat catacagagg aaatcgtcgg
    15181 agtagcgtgg gggacagaaa agaaaattta tgtcacgaac gatctatctg tattcgaaaa
    15241 ccgagcgttc cattcttgga tcactgatcc gactcggctg aaaaaagtat atgatgcgaa
    15301 acgaacttat gttgctttaa accgctatac tggaaaaacg aaaggaatcg attttgatgt
    15361 cttgctaggt gcataccttc tcgatacgaa tgaaaaaagc acagatatcg aaggaatagc
    15421 tgctcattac ggatacaacg atatccaatc agatgaagct gtatacggca aaggggcgaa
    15481 aaaaggttta ccagaagaag aggaactctt ttttgctcat ttagcgcgaa aagtagctgc
    15541 aatcaatgcg ttgacaccaa aattgagcca agaactggca gaaaaaaacc aagaagatct
    15601 tttccggaaa atggaactgc cattgtctat cattttggca gagatggaaa taagcgggat
    15661 caaagtagat gcgactcgct tacaagaaat gaagggcgag ttctctgctc gcttaagaga
    15721 gatcgaacaa aaaatctacg aagaagccgg cgaagaattc aatttgaatt caccaaagca
    15781 attaggagtg atcttatttg aaaaaatggg attacccgtt atcaaaaaaa caaaaactgg
    15841 atattccaca gccgtagatg tactggaaca gcttcgtgaa caagcaccaa tcgtagagga
    15901 tatcctcacc tatcgtcaaa ttgcaaaaat tcaatctacc tatgtcgagg gactgctgaa
    15961 agtgatccaa tcagatggaa aagtgcatac ccgttatgtc cagacattga cccaaacagg
    16021 acgattaagc tctgttgatc ctaatttgca gaacatcccg attcgattag atgaagggag
    16081 aaagatacgt caggcatttg ttcctagaga aaaagactgg ctgatttatt cttctgacta
    16141 ttcacaaatc gagttacgcg tacttgcgca tatctcaaat gatgaacatt tgaaagctgc
    16201 cttcctagaa ggtcaagata tccatgccag cacagcgatg cgtgtatttg gaatcgaaaa
    16261 ggcagaggat gtcacaccaa atatgcgtag gcaggcaaaa gcagtgaatt ttggtatcgt
    16321 gtatggcatc tctgattacg gactttcaca aaatctagga atcagccgaa aagcagcaca
    16381 gcaatacatc gatacgtact ttgaaaaata cccaggtgtc aaagaatata tggaaagaat
    16441 cgtacgcgaa gcaaaagacc aaggctacgt cgaaacgctg tatcaccgcc gtcgatatct
    16501 gccagacatc aattcaagaa attacaatat ccgttctttt gcggaacgaa cagctatcaa
    16561 tacaccaatc caaggaagtg ctgctgatat tttgaaaatc gcgatgatcg aattggataa
    16621 acgattaaaa gaaacaggtc ttcaagctac gatgctgtta caagttcatg atgaacttgt
    16681 cttcgaggta ccggaaaaag aacttgaatc attagataaa ttggtgaagg aagttatgga
    16741 acaagctgtt tctcttcatg taccactgat cactgacagc agttggggaa aaacgtggta
    16801 cgaagcaaaa taaacattgt ttaagacaga gcaatataat gaaggtgaaa agaaaaaatg
    16861 ccagaattac cagaagtaga aaccgttcgt aaaggattgg aacggttagt agtaggaaaa
    16921 acgattcaaa aagttcaggt cttgtggccc aagatcatcg agcaaccaga gacacccatc
    16981 tttgaggctt ctcttgttgg cgaaaccatc caatccattg gacgtagagg gaagttttta
    17041 atttttcatc tcgatcattg tgaactgatc tcacacttgc gaatggaagg aaaatatcaa
    17101 tttacgaaag aaaatacacc aatcgataaa catacgcatg tacttttctt ttttgaggat
    17161 ggaagccaac ttcgctacaa cgatgtacga aaatttggtc gtatgacaat cgtcgaaaaa
    17221 ggagcaagtg caacttaccg aggaatcatg aaattgggac ctgagccttt gccagatagc
    17281 ttcttattag cagatttcgc aaatggactg aaaaagtccc acaaagcaat caagccattg
    17341 ctgctagacc aacgtctagt gactggtcta ggtaatattt atgtagatga agcattatgg
    17401 gaagcgaaga tccatccaga acaaccggcg aacacactgc gccccaaaga ggtagaacaa
    17461 ttacgtctat cgatcattga tgttttggat cgtgcaatag aagccggagg taccacgatt
    17521 cgcagttatt tgaatgcatt aggagaatca ggcggatttc aagtagcact gcatgtctat
    17581 cagcaaactg gcaaaccatg tatacgttgt ggaacgccga tcgtgaaaac aaaagtggca
    17641 caaaggggga cgcactattg tccgaattgt caaaggctaa aaatcgcaaa gtaggatttg
    17701 ttttaggttt gacgggtgga atagctaccg gtaaaagtac ggcagccaaa gtctttcagt
    17761 ctcatggatt tcctttgatc gatggcgatg tgattgccag agaaactgtt gagcctggaa
    17821 cgcttgctct gaggaaaatt gtatcagtgt ttggccaaga ggtattgcag cctgatgggc
    17881 aactagatcg agggaaactt gggatgattg tttttccatc aaaggaaaaa cggcaaaaac
    17941 ttgatcagct attagatccg tttatccgaa aagcgataaa agatcaaatc gcctctctaa
    18001 gttccaatca tcctttagta attgtagata tcccattgct ctacgaaggt cattatgacc
    18061 actatatgga tgcagtagct gtcgtttata ctacgccaga aactcagctt cagcgtttga
    18121 tgaaacgtaa tcaactgacg ctagaacaag cgcagcagag aattagtagt caacttccaa
    18181 tcgaagaaaa aaagcagcgt gcggatattt tgttcgacaa caatggaaca aaagaaatac
    18241 ttgtgggaca aatcgaaaat tggctttttg ctaatcgctt tctttgagta tctgtgtcaa
    18301 ttttgctatc ctgagtatat agaaagtgag ggatagcgat gggatcatta aaaaatcaaa
    18361 aggataaagt cgtaggtaac gcaaaagaaa aaatcggcga gatgctcaat aaagaaaatc
    18421 taatcgatga aggtcgtacg caatcagaaa cagcagaaga aaaagaaaag cagtaccaag
    18481 caagaatcga tgaaatagaa gaccagcagg cagaagaaga agcgaagcgg caagatttga
    18541 ctcaagaacg aaaaaatccg acacctgatc gtagtaaaac accgctccca gaaagtgacc
    18601 acattcccga tgagcaaaaa atcgaggaac agcgaatgaa acactataca ggaatcgaag
    18661 agaagttata aaatagtaaa aaatcgagat gtgacttgaa gcagatgaga aggtgttcat
    18721 ctgcttcaag ttttttgtgc tagatgacta aatcagcttt tgtgtaagtt atttctcagt
    18781 aatcaaggtg atcagtattg gaaaattgaa atttagtaag gacaggggac acaattacgc
    18841 atgaaagtag aaaaaattgt tcttatatcc gtgttttttg ggtaggacag tcgcgtttgt
    18901 gatttgtttc gtgttataat acaactagtt gtaaagaaag acacatcctt tatataaaat
    18961 gcgatgcaag aggtgagaca aaatgagatg tcccagatgt caacataata attcacgagt
    19021 aatcgacagc cgtcaagcag atgatggacg tgcgatccgt cgccgtcgcg aatgtgagaa
    19081 ttgttcgttc cgattcacta catttgaacg aattgaagct gctccgttat tagtaatcaa
    19141 gaaaaacggg gaacgcgaag agttcaatcg cgataaaatt ttaagaggac tcatccgttc
    19201 agcagaaaaa agacctgtag ctatggaaca aatggaacaa atcgtcaacc atgtcgaaaa
    19261 ccgcgtacgc agtctcggag aaaacgaagt atcgacgaat ctaattggtg aatatgtgat
    19321 ggaagattta gttgatttag acgaaatcgc ttatattcgt tttgcaagtg tttatcgcca
    19381 gttcaaagat atgtctgttt tcttaaaaga gttgcaagat atcgtggata aagcaaaaga
    19441 aaataataaa gagaataata aagaaaacaa ttaagaaaca aaaaggaggc atttccttgg
    19501 aaaatgcttg gaaagagctt caaccaaaaa atatttacca aggaagaaaa gcggctccgc
    19561 tgagagaaga cagcaagaac gccttgctgt atctttatca gccactaatc ggcggagaag
    19621 ccttgagtat ctatttcacc ctgttatcag aaatctctca agaatctggc aaaggtcctg
    19681 aagggatgca tgcagatctg cttagtagtc taggctgcgg tatcccgcaa ttttatgatg
    19741 caagaaaaaa gctagaagga atcggcttac tggatgttta ttttaaagaa gatagtgatc
    19801 tgggacctcg ttttatttat gagctgttag aaccactcat tcctaaagca tttttcaaag
    19861 actctgttct ggcttttctt cttctggaaa aagttggtga acgacgattc cagaagctta
    19921 cccaattctt tgaacctaaa aggcatacag cagagggata tcaaaaaatc acaaaaaaat
    19981 ttttagatgt ttatcgcttc aatgaagcag tgtacgctgc taatcaagaa aacatcgaat
    20041 cgctgaataa atcttttgaa gaatctgaat cagtagcatt acttgaagca gattcgacat
    20101 tcgattggca gtttttgaat gattggctgg caaaaaaaca tattgcaagt cttgatgatc
    20161 caatcatcaa acaagtcgac atgtatcatc agctttacgg tttcgatgaa ttgacacttg
    20221 gcgaactgat cagtcaatct tatgacttta gtgaacaaaa agtatctgaa aaagaactgc
    20281 aaaaagccat tttggcaatg cagagacgtc cggtgaagca aacaaaagaa acagttgcgg
    20341 tttctgaaaa cagtaatgaa ccctcgtcaa acgaacattt gacaaacgca gcgcttcagc
    20401 tgataaaaga agcacagagc attccaccga tgaaatatct ggaagcaatc aagtcagaaa
    20461 aaggcggata tgtttctaaa caagaaaact ggttactgca agatcttgtt tctcaatcag
    20521 gtctttccag tagcgtgatc aatgttttgc tgaattatgt actagtgatc aagaataatg
    20581 ctagcttaaa tgccagttat gtaaatacag tggcaaatga atgggcacaa aagaaaatcg
    20641 ccacagcaga agaagcgatc cagcacattc ggcaaataag taatcaagca aaacaatcaa
    20701 aacaaaagaa gcaaacaaat tatcaaactg gcaagcgaaa tgttcgacgg gaaaagcttc
    20761 ctgattgggt caaccagcca aaagatgaaa aacagatcag cagcgagaaa aaagcagaga
    20821 ttgaccgccg ttttaaagaa tacctggcaa aaaaggaggg tgatatctag tggaagacgt
    20881 aggcaaagaa ttgaagagga tcatcagcaa acgcaattat caagaaaagt atgctgaaat
    20941 gatcgctgaa gtcttaaatg atgcggatgt tcaagctttt ctaagcgcac accaagaaga
    21001 attgacacaa gcggatattg aaaaaagtta cgctaaattg tatgaattcg tgcaagaaaa
    21061 gcgtaaatat caaaaaaacg atccaacgat gatcgctccc ggctatgagc cgaagctgac
    21121 gttgaatttt cattatgtgg atgtcactta tgtaccaacg gaagcattgt tagcacggca
    21181 aagacaagaa gaaatcagaa gccgagtaaa agcaatggat atgccaaaag atattcaatc
    21241 ggcttctttt gaaaactttg aacgtacaga tggacgagga tatgcgagct tagaagccct
    21301 agattttgta gagaaatata ctgctgatcc aaaagggtat cataaaggac tgtatctagt
    21361 aggaagtttt ggtattggaa aaacatattt attaggagca acagcgagag aattagcgat
    21421 tgctggattc accacgacgc tagtccattt tccgacattt gccgttgaga tgaaacaggc
    21481 aatcggaaaa gatcaagtgg cagaaaagtt agatgcagtg aaacgttcac ctattttgat
    21541 gatcgatgac attggtgcag atgcgatgag tagttggata cgagacgaag tattcggtgt
    21601 tatccttcaa tatcggatgc aggaacagtt accaaccttc ttctcatcga attttacaat
    21661 ggatgaattg gaacagcatt tgtccgtcac tcaacgtgga gatgaagagc cgctaaaagc
    21721 gaaacggata atggaacgca tacgttattt gacaaaagaa atcgaaatga ctggtcgcaa
    21781 ccgacgcaat ggttgaacag ttgacaagtc caactgacat ttgacctagt agtaaaagca
    21841 ggtcagcgaa tcattatatg gacagaaatg accggagaca atcaccgcaa caagtaatgc
    21901 agaaacttaa acggaggatt ttttatgagg gctatttttt ctagtatatc tgtagttggt
    21961 ctattttttc ttttagcagg ctgcggtagc gcagatagca acacaggttc tgttgattcg
    22021 attgatgcgg gtcaaacagt aaaaacaagc agtacgaaaa cggagagaca aagcatattt
    22081 gtcggaacga ttgaaagtat cactcaagta gaagcagaag aagaaacgct tcagttgaaa
    22141 ctcgtcgatg tcatggccaa agaagatcca gcgaatatgg gaacgtcatt cagcaatgac
    22201 ggagtgatct taaatgctac tgctgaaaaa ataagcgtgg acataaatga attgaaaatc
    22261 ggtgacaaag tccaatttac attagttgag atgccgatca tgacgatgtc tatcccaccg
    22321 caagttcctg gagattcgat tattgatgtt tcattgaaca aataaccaat aacatttttt
    22381 caaaaataag aaaatgttgt tttatagaac gaaaaggtat attttttaag gagatatggg
    22441 aattttttta ttcctagtct ccttatttta tttggaaaat gctataatta tctagaagga
    22501 gtgaagctat gaaccattta gatttatctc agcgcttagc cgcagtgggt gactttgtcc
    22561 ctgaatccgc tcgtttagca gacattggat cagatcacgc ttacttaccc gttgcattga
    22621 tattaagagg aaaaatcgaa tatgcagttg ctggtgaagt cgttcaaggt ccttatgaat
    22681 cagcacgtaa gcaagtcaaa aaagatggat tagaagaccg tatcaccgta cgccttgcaa
    22741 atggtctaga tgctatccag ccacaagatg gtattacagc tgtcacgatt gcgggtatgg
    22801 gaggttcact gatccgagat atcctagaag ctggcaagtt gaatcaacgc ttaagcggac
    22861 aagaacgttt gatccttcag ccaaacatcg gagagcgtac attacgatct tggctgcaag
    22921 aaaatcagta taaaatccaa tcagaaaaaa tacttgaaga aaataaaaaa atatatgaaa
    22981 tcattgtagc cgaaaaagaa caaaaaccag ttacttacaa tgaaatggag ctgatgttcg
    23041 ggccatttct gcttgaagag aaaagtgaga tcttcaaatc aaaatggaca agagagcttc
    23101 aacaaagaaa agctatccgt gaacagctca aacaagcagc taatcaagag gcacgtcaaa
    23161 aacaaatcga gcaggaaatc caaatgattg aggaggtttt aaagccatga caatcagcgg
    23221 acgcactttc atccaaaagt ttgaaagcta ttgtcctcag tggctagctg aagaaggaga
    23281 cccagtcgga ctgcatatcg gaacattgga taagccaatc caacgagtaa tgatgacatt
    23341 agacgttcgt ccagaagtgg tcgaagaagc aatcaagaaa aaaatcgatt tgctgattgc
    23401 taaacatcct ccaatctttc gtcctgtaaa acggctggtt actgatcagc cacaagaaaa
    23461 aatgtatgca gatttgttga aacatgatat tgccgtctat gcagcacaca cgaacatgga
    23521 tattatttgg gatggactga atgactggtt ttgtgaactt ctcggtattg aagtggagag
    23581 ttatttagta aagactcatg agattcatta taaaaaactg gccgtttatg tacctgtgga
    23641 tcatgcacag aaaatgcgtg aagtattagc tgcagctggt gctggtacac aaggtgatta
    23701 taccggtaca agttttacct cgattggaca cggtcgattt actccggaag ctggtgcaca
    23761 accagcgatt gggaaagtag ggaagacaga acaagttcaa gaggcaaaag ttgaggttat
    23821 tttgccggaa actatcgaaa aacaagtgat tcaagccatg cggtcagctc atccttatga
    23881 ggaacctgct tatgatcttt tcgctataga tgaaccagtc gaaatgtttg ggttaggacg
    23941 agtaggtgaa ttgccgcaag aaatttctat agaggcattt gtagaacaag taaaagaagc
    24001 gttccaacta gatggactga gaatagtcca gccaaaaaat gcaaaatcct ctgtgaagcg
    24061 cattgcgatt tgcggtggaa gcggtgaaaa attttatccg caagcaatcg cccaaagagc
    24121 agatgtctat atcacgggag atatctatta tcatacggct catgatatgc aaagcgctgg
    24181 gttgattgca attgatcccg gtcattatat tgaatcgtta tgcaagcaac gatttatcga
    24241 gaaatttgaa tcttggaaac aagaagaaaa ttgggatatt aactttttcg tttctgaaac
    24301 aaatacaaac ccatttcaat ttaattaaaa ggagagaata ctatgtacga aaatcttttg
    24361 ccacgttttt tacgatatgt caaaacagaa acacgctccg atgcaacaag caaaacgact
    24421 ccatcgactc agacacaagt tgcttttgcg caagaattga aaaaagaatt agaagagtta
    24481 ggcttagctg atgttttcta taatgaagat aacggattcg ttatgggaac attaccaagc
    24541 aatgttgatc atgaagtgcg ttccattgga tttatttccc acatggatac agcagatttt
    24601 aatgcagtag gagtcaatcc acaaatcatt gaaaactatg atggcgagtc tacgatcgta
    24661 ctagatgcgg aagaacgcta tacattgaat acaaaagatt ttccaaattt gaaaaattat
    24721 gaaggccaca cattgatcac aacagatggc agtacattac tgggagcaga tgacaaatca
    24781 gggattgcgg aaatcatgac tgcaatggag atcttgatca ataatccttc tatccctcac
    24841 ggtaccatca aagtggcttt tggtcctgat gaagaaatcg gtgtcggagc ggataaattc
    24901 gatgttgagc aattcgatgt agattttgct tatacagtcg atggtggtcc tttaggtgag
    24961 ttgcagtatg aaacgtttag tgctgctcaa gcaaatatta cgatccaagg gaaaaatgta
    25021 catccaggga cggcaaaaga tacaatgatc aatgctctac agttagcaat cgattttcat
    25081 aatcagcttc cagccgatga agttccagaa aaaacggaag gatacgaagg tttcttccat
    25141 ttgatggcat tggatgggaa tcctgaagaa gcttctatga gttatatcat tcgcgatcat
    25201 gatcgtgaaa aattcgaagc acgcaaagca caaatcacac atatccaaga agagttgaat
    25261 cagcgatttg atcaagaacg aatcaaagta gatatgtatg atcaatatta caacatgaaa
    25321 gaaatcattg aaaaagatat gagcatcgtg gaattagcga aacaagcaat gatcgagctt
    25381 ggtatctctc cagtgatcga accagttcgt ggaggaacag atggttcaaa aatctcttac
    25441 atgggtattc cgacgccaaa tctatttgcc ggaggagaaa atatgcacgg acgattcgaa
    25501 tttgtttctt tacaagtaat ggaaaaagca acagacgtga ttgtaaaaat tgcagaattg
    25561 aacacaaaga gttaaaatcg aaaaagaaca gcttatgaca aggcgttcgt cccaatgatg
    25621 gaatgtcatc tgtctaaggc taaagggcag gacaatggaa ttggttgtcc cgccctcttt
    25681 tttgaagagt aaataggagg taaagaatgg aaaagctgct agttttttat aacaaaagtt
    25741 cagggaaaga tgagggtgag aaattagcac gctggtttca agattatgta aaagaaaagc
    25801 agccagaatt atatgtagaa ctagctgaaa ctggcccatc gattgatgat gcggaattag
    25861 tcaagaaagc agaagccata gaagctgata ctttagtaat tataggtgga gacggcacga
    25921 ttcatcatat cgtccaagcg ttccaaaaaa cattatctca ataccatgtc ggattacttc
    25981 caggtggaac tgtcaacaac cttgcacgtg ttcttgagat tcccctcaaa aaagaagaag
    26041 ctgctgacgt gatcctggaa ggcaatgtac ggaaaataga ctatggtcaa gtcaatgatg
    26101 aagtcattat ctctacactg acaatcggaa tcttagcaga tacagcagtt tggatcagtc
    26161 aagaagaaaa acagcgttac ggctcgtgga tctttatcaa gaagtttttt aagttattgc
    26221 tgaagaagaa aaaatatcat ttggatttag aaacagacga ggagcattgg aaaggaaaat
    26281 cccaactgtt gacagtcacg atgtctaact ccgtgggcgg ttttacaaat tttgacgact
    26341 cagcgaccct agatgatgga aaatttcatg taacgatcgt accttcctta gatattttcc
    26401 gatttatttt ttatttgcct agaatcatga gaggcaaatt ctatacgatc cccggcatta
    26461 cttatttcac agccaaacgc atgaaaatcc aagcgcagga aaaatctgtg ggcactcgaa
    26521 cggatggaga cacgacagat gatcttccag ttgaattatc tgttgtatca aaaggattaa
    26581 cagtttattc accaaagaaa gaagaaaaag cttagaaata gtagttaaca gaaagttgga
    26641 agaagagtaa tggatgtatt gttacacaat cctttgtggt tttacctttt cattgtgaat
    26701 ttttacttat tttgtctgat ggggtatgat aaacatcagg ctagaactag cggctggcgt
    26761 gttcctgaga ataatttgat atttgtcagc ctgttaggcg gtggtttggg aggattgatc
    26821 tctcgaaaag ttttccatca taaaactcgg aaaaagaagt ttactatagg attcgctgca
    26881 ggtgtacttg ttgatttgat tctcatttat tttttccatt aaggagcagg agatgtcgtc
    26941 taaaggaacg aaaactaaaa tcattctgaa tctgcttttg ctgattgtga tttttgccat
    27001 tatttattat ttaatcaatc aatcatttgc agatatcttt gccgaattgc tgtctaccag
    27061 tgttgttgtt ttggcagcgg ctgttatatt agggacagtt taccaaatcg ctgaagggcg
    27121 ctcaatcaaa gaaattgctg aacctttcgc gccgaatttt acaacgaaag atgggttctt
    27181 gacctctagc tatattgcgt tttaccgaat cgtttcgttt ggtacaggaa cgctgatctc
    27241 ggagatttat ttttataaca aaaaaggaat gaagctatcc cagggagcag gggtgacagc
    27301 actccatatg attatgtaca aactggcagt gatcacttat gcattagctg gcttggtcat
    27361 ccaattctct cttttttatt ctaatggtcc atcaatgatc tggtttatca tcgctggtat
    27421 ccttttgacg atcgtgattg ttgccttttt attggcgatg tccgtcagct tgaatgttca
    27481 aatatttttt gtcagcttat cgaataagtt atttaaaagt gagcgtttgc ggaaatgggt
    27541 ggatacatgc aatcagcaga tctactcttt aagggaaacg gtccaaacca ttatccaaga
    27601 caggactgcg ctattgcgga tttatctatg gaatatgtta aaacttgtat tttggtatgt
    27661 acttccttat ttgattctga ttgaaaacca tcctaaaata gattttctac tgaccatttc
    27721 gtttatcagc tttgcagtag tgctttcagg ggttattcct acgcctgctg gtattggttc
    27781 ctttgaattt gtctatttgt tattattcag accagttgtt ggaacagttg acgcagtttc
    27841 ttcgctttta ctgtatcgtt tcagttcctt tatcttgcca tttctctatg gatttgtgta
    27901 tgtgattgct gaacggcgaa gagtgatcaa agaagaaata atagaagtaa aaaaagagaa
    27961 taaagaaaat caagggtagg tcttttataa gatctaccct ttttatcaaa tcaaagaaaa
    28021 acgttatgga taagataatt ttacgtttgt ttggctcttt ttttatatac atacaatcaa
    28081 ttttgttttt ttaagattta tataagtcga gaaaaaaagt caaaaaacta tgtgtatatt
    28141 ttgaccaaaa ttgaccaaag cgatataata gtttttgtaa agatcaaaga aataagtgat
    28201 cttaccttta attaggaggg tatacctgta tgaatattga gaaaatgact acgacgcttc
    28261 aagaagcgat tgctgaagct caacagattg cggtcactcg aaaacatcaa gatatcgaca
    28321 ttgcccatgt atggaaaatc tttttacaat ctaatcattt tggtcgaaat ttctatacgg
    28381 atgccggatt ggatgtcgaa tcttttgaac atgaaattga tcgtctactg gatgagtatc
    28441 cagttgtttc tggtggaaac gtccaatatg gacaaaacct cagccagaat ttctttcgat
    28501 tattgaatga agcagaccaa atacgtgaaa gcttcggcga cgagtttctt tcaacggaag
    28561 tcgttatttt agcgttgatg aaattaaaaa attatccgtt aactgtttat ttgaataaaa
    28621 acggattatc tgaaaaagaa ttgcgtaaaa acatcgaaga gatgagagga ggagaaagag
    28681 tgacctcaca aaaccaagaa gaacaataca aagcattaga aaaatacggc gttgatttag
    28741 tacagcaagt caaaagcgga aaaatggatc caatcatcgg tcgtgatgaa gaaatccgtg
    28801 atgtcatccg tattctttca cgaaaaacaa aaaataatcc tgttctgata ggagaacccg
    28861 gtgtagggaa aacggcaatc gttgaagggc ttgctcaacg tatcgtacgc aaagatgtgc
    28921 ctgaaaattt aaaagataaa acgatttttt cattagatat gggtgcattg atcgctggag
    28981 caaaattccg tggtgaattt gaagaaagac taaaagcagt cttgaaagaa gtcaaaaaaa
    29041 gtgatggacg tatcctcttg ttcatcgatg aaatccacaa tatcgtaggg gcaggaaaaa
    29101 cagaaggaag tatggatgcc ggaaatctgt tgaaaccaat gcttgctcgc ggtgaattac
    29161 atttgatcgg tgccacaact ttagatgagt accgtcaata tatggaaaaa gacaaagcgt
    29221 tggaacgtcg ctttcaaaag gtattagtca aagaaccaac agtggaagat acgatcagta
    29281 ttctgcgtgg attaaaagaa cgttttgaga tccaccatgg tgttaacatc catgacaacg
    29341 cattagtagc agcagctact ttatctgacc gttatattac tgatcggttc ttaccggaca
    29401 aagcaatcga tttgatcgat gaagccagtg caacgattcg ggtagaaatg aactctatgc
    29461 caacggaatt agatcaagtt actagacgtt tgatgcagtt ggaaatcgaa gaagctgcac
    29521 tgaaaaaaga atcagatgat gcaagtaaaa aacgcttgaa aaatttgcaa gaagaattag
    29581 ctgaacttcg tgaagaagca aatgcgatga agatgcagtg ggaaacagaa aaagaagaag
    29641 tcaattctgt ttcagcaaaa cgtgctgaaa tcgataaagc aaaacacgaa ttagaagatg
    29701 cagaaaataa ctatgattta gaacgcgctg ctgtattgcg tcatggtacg atacctcaat
    29761 tagaaaaaga attgaaggaa ttagaagcta aagcaaaaga cagcgagatt aagatggttc
    29821 aagagtccgt taccgaaaat gagattgctc aagtcgttgg cagattaacg ggtatccccg
    29881 taacgaaact tgtagaaggt gaacgagaaa aactaattaa attgaatgaa acacttcata
    29941 aacgagtgat tggtcaagat gaagcagttg atgcggtcag tgacgcggta attcgttcac
    30001 gggctggatt gcaagaccca aatcgtcctc taggttcttt ccttttccta ggaccaactg
    30061 gtgtcggaaa aaccgaatta gcgaaagcgt tagctgagaa cttgtttgac tcagaagatc
    30121 atatggtacg gatcgatatg agtgaatata tggaaaaaca tgctgtgtct cgtctagtcg
    30181 gagctcctcc aggctatgta ggttacgaag aaggtggtca attaacggaa gctgtcagac
    30241 gaaatcctta tacaatcgtc ttgttggatg aaatcgaaaa agcgcatccc gatgtcttca
    30301 atatcttgtt acaagtccta gatgacggac gcttgactga ttcaaaaggg cgagtagtag
    30361 atttcaaaaa tacggttctg atcatgacca gcaatatcgg ttcacagtta ctgctggaag
    30421 gcgtgactgc tgatggaacg attccagaag cagtggctga acaagtcaac acgctattac
    30481 gtggaaactt caaaccagaa ttcttgaacc gaatcgacga cacgatttta tttacaccat
    30541 tgagcctaga caacgtgaaa ggaatcgttg acaaaatggt ggcgcaattg gcacaacgtc
    30601 tggaacatca agaaatcctg ctgacaatca gtgatgaagc caaaacgtgg attgctgaaa
    30661 atgcttatga accagcatat ggtgcaagac cattgaaacg atttatcact aaggaagtag
    30721 aaacaccact agctaaagaa atcgttgcag gacatgttat gcctaaatca aaagtaacga
    30781 ttactttact tgatggacaa cttcatttca aaacagaaga gctagaagaa atcgtctaat
    30841 cttccattga gactataaaa gaaaaaaggg gtctgacaca actagtgtca gacctccttt
    30901 tttcaaataa acagtgttcc agaagtagtt tcttcgaaaa ataagccgaa atttcttgaa
    30961 gctgcccgct tctgtcacaa cctcttttat taggtatttt tcactttttc taatttataa
    31021 ctttcaaagt aacgctcatc ttttttactt tgagaccatg caaacaatcc cattaaggca
    31081 gcacctatcg tatttgtcag caagtcgccc atcgtatcca tcaatgctgc tcgtccgaca
    31141 aacagtgtgc catcagaagc agcaaaacgt tgaagattca tgcctaagaa ctggtcgcat
    31201 aagaattccc agaactccca aaacacgccg caaagtccag caaacgcaaa gccgaataag
    31261 aggaacaacc aaggactggt ttttgatatc tcagcatctt tcatcaagta accaatcaat
    31321 ccatagccaa gagctgtcag gaccattgga ctcaccgcat gtaagatttt gtcccaaaaa
    31381 gagatgatac tgatcaaatg catccctgtg cctaaaaaga cagagatgaa caagaaaaac
    31441 cagtaaaaca aaacgattgc tgctggtatt ttaagtttga aaactttcaa aatgacttgt
    31501 ggcatgaaaa tcaatgcaat ccctaataaa gattccacga taaatgtgat tccttgagaa
    31561 gtcgaatatt tttgtgtgat aaattcataa aaattatagc agacaacgaa tacaccaaaa
    31621 gcaagtaaag ctatttgcac ctttttgatt tctttaatca ctgaaagtcc ctccatctaa
    31681 aaatcttttt acttctgaaa gcaactcaat ttctgaagaa accacttttc cgttcaattt
    31741 gatcaaacct attgtatata agttcaggta gtgaaattga ttttcagctg tttcttgtag
    31801 tgcagcgatt tttttcgggt gggttccgcc ttgctgtcga gtatcagtat ataagccgat
    31861 cactgggatg cctttcgcat aagccacacc gatttccgat gctaccccgg catcgatcgt
    31921 cagcccatct agtaaagcaa tcattaaatc actagccaac actttttctg tgtcagcaag
    31981 agcaatcatt cgactgtctg cataagcagt tttgtcattg attgcttcat tttcttgagg
    32041 aagatagatc gaaagctcag aagagagctc tctgatttct ttaactaact gttggttata
    32101 taacaaatca cttttagcaa acattggtgc ggcaaaataa attttcatct aaacatcatc
    32161 ctttttatca aaaataatcg ctaaagtaag cttccatgtt tggaatgata cgctctagtg
    32221 acttcagtgt ttcttcatct gtttctaacc gttcgattct tgccaaataa gaagcacggc
    32281 gataattcat catcaagcaa gtcaatgttt ggatatccag tcgaacggat tttcctatag
    32341 gttcatttaa gacacggact tgtccctgtt catcccatgt taaagcgaag acaccattat
    32401 tccaaggagc gacaggatct tcaatgatga aatgaaaagc atcagctgtt ccaacaaacg
    32461 gaaaccgttg aaggaattct tttacatcaa cgatccgagc cataaaatat ggttcgatct
    32521 gctctttgat ctgactatct tcaatcaaaa atgctaaagg ctcattttta taaatatctc
    32581 catgtaccca gtagatcatg gaaaaatgtg cggaaataaa gttccataag ccatttcgag
    32641 cttcttgatc aagatagaac atttctttta cgtgaaatat ttcttcagct acccagtaga
    32701 acagaacacc tcttggttca tggttgctat cgtagtagac agcggctgtg cgctcatctt
    32761 cattttcgaa acgccaatac tcttcccaat gaaaatcact gcggaataaa gcaccatggt
    32821 tttgttgggc aaatttgtca taaacggaaa atacgtctgg atgatcaact ggtaatcgct
    32881 caaccatacc acttactggg atttgtttag gtagctgtgt atctctgatt ttaaaggaaa
    32941 gtttatcaga catgatttcc catcctttac gccgatagta ggggatactg taaggataca
    33001 aataagagat ccattgctta ttcttgcgca tattatccaa tgcaacgata attagatctt
    33061 gcatcaatcc atggttagca tattctggat aagttccaac tccagtcaca ccacccattt
    33121 tataacgtgc accgtgaata ttgacttcac aaggatagat agcgatttga gagatcagtt
    33181 tattttcgtg gaaccatccg aacactttcg atacttccaa aatcggtttt ttggaacgga
    33241 taaaagctcg tttattctca aacccgcttt cctcgatgtc tgcttcagtc acttggaaca
    33301 cataggaaag caattcattg aattgatcga tatgttcttg agtgaccggt tttaattcaa
    33361 gattttctct aaaatctttt tgatccattt tttcatctcc atttattagc ttgataacag
    33421 tatagcaaaa ttctacaaaa aaaacttcta tagctgacta attaaaaact ctttaaatta
    33481 ctgcttttcg ttggtataat ggaaagtgct gattatatga aaagaggaga cgctatggat
    33541 ataaagaaaa tgaaagaacg ccaagagcag attcgtaatt tttcgatcat cgctcatatc
    33601 gaccatggga aatctacatt ggccgatcgt attttagaaa tgacgcacac tgtgacgtct
    33661 cgcgaaatgc aagatcagct gttagactcg atggatttgg aacgtgaacg tggaatcacg
    33721 attaaattaa atgctgtaga actcaattac acagcgaaaa atggagaaac atatatcttt
    33781 cacttgattg acaccccagg gcacgtcgac ttcacctatg aagtctctcg aagccttgct
    33841 gcctgtgagg gtgccgtttt agtggtcgat gcagcacaag gaatcgaagc acaaacattg
    33901 gccaatgttt atttagcgtt agacaatgat ttggagatcc tgccagtcat caataaaatc
    33961 gacctacccg cagcagatcc cgaacgtgtc cgtcaagaaa tcgaagacgt aatcggaatc
    34021 gatgctagtg aagctgtatt agccagtgcc aaatcaggga ttgggattga agatattctc
    34081 gagcagatcg tggaatatgt tcctgcacca agtggagact tagatgcacc gctaaaagct
    34141 ttgatctttg actctattta tgatagttac cgcggtgttg ttctaaatgt ccggatcacg
    34201 gacggtatgg tcaaacccgg agacaaaatc aaactgatga gtaatggcaa aacatttgat
    34261 gttacagagg tcggggtctt ctcgccaaaa gcagttgcta gagacttttt gatggtcggt
    34321 gatgtcggtt atatcacagc aagtatcaaa actgtccaag atacacgagt aggggataca
    34381 gtaacgttag cagacaatcc tgccgctgaa gctttaccag gttaccgaaa aatgaatccg
    34441 atggtttatt gtggtcttta tccaattgat acttctcgtt acaacgattt gcgggaagct
    34501 ttagaaaaac tgcaattgaa tgatgctgct ttacaattcg aaccagaaac ctctcaagca
    34561 ttaggttttg ggttccgctg tggattcctc ggacttcttc atatggatgt cgtccaagaa
    34621 cgcttggaac gggaattcaa tctagaattg atcacgactg caccatccgt tatttaccat
    34681 gttaataaaa cggatggtac gactgctaca gtagataacc cggcagattt tcctgaacct
    34741 gtaacgattc aggatgtaga agaaccattc gtcaaagcac agatcatggt cccaaatgat
    34801 tttgtcggtg cagtaatgga actttctcaa cgaaaacggg gagaattcat tacgatggat
    34861 tatttagatg attaccgagt aaatgtcgtc tataatattc cgctttctga aattgtcttt
    34921 gactttttcg acaaattgaa atcaagcacg aaaggctatg cctcattgga ttatgaaatg
    34981 tctggttatc aaaaaagcaa gttagtgaag atggacatct tgctgaatgg agaaaaagtc
    35041 gatgcattaa gctttatcgt tcaccgagac tttgcctatg aacgtggaaa agcaatcgtt
    35101 gaaaaactga aaaaactaat cccacgccaa caatttgagg tgccaatcca agcagctatt
    35161 ggacaaaaaa tcgttgcccg ttcagacatc aaagccttgc gtaaaaacgt cttggctaaa
    35221 tgttatggtg gagatgtttc tcgtaaacgt aaactcttag agaaacaaaa agaagggaag
    35281 aagcggatga aacaaatcgg ctccgtcgaa gttccgcaag aagccttcat ggccgttctg
    35341 aaaatggacg aggacgaacc gaagaaatag caagggtttg tttgaaaaca gtgtaaaaaa
    35401 taaaccatgt ttacatggat ttgccaccgg tttgccaccg gtggtttttt tcggtggcaa
    35461 aaatcaagaa ttctttctaa gattttccat gatatcgacc gtttcatttc tcatcttatc
    35521 tgttacatgc gagtaagtat ccatcgtaat tgaaattctg ctgtggccca atcgttcaga
    35581 gatttctttc atttttgcgc cattttcgag aagaagtgta gcatgagtat gtctgagaga
    35641 atggaagtta aaagagaggg agagtgcatt cgatatcctc ctagtattcc atttcactac
    35701 acttggcgta actagctcac cgtcttcctt cgtacatact gcatttgaat caatgtagag
    35761 ctttccatac ttcattcgat tttctaattg ttgtttctta tgttttttca gaattgctag
    35821 caaggtttgt ccaataaaaa tcgttcgatt ggaactgctt gtctttggcg taccatatac
    35881 ccatgcgccg tcattcttta ccatttgttt ctctacagta attgttccat ttgaaaaatc
    35941 gacatcatcc cacgtcagac cacaaacttc gccaacgcgc attcccgtat aaaatccaat
    36001 attcaaagga atatagaaag gatgtccttc aggagtaatt tctagcatat gatcaaagtc
    36061 ctcaagagaa atgattttta gatctttttt agtcgttggt cgtgcttcat attttggtat
    36121 ttttacatac agcataggat tttgcttgat taatccccaa ggataaaccg ccatattcag
    36181 cgcattctta aggacagagt gagtaatagt cattgttttc ttcgagtaac cctttttaaa
    36241 ttcagcattg atgaaatttt gtaaaagagc aggggagaga tccgtaagtt ttttctttcc
    36301 taaataaccg tttatatgat ttttgatggt aaatcggtag ttttcatagg tattgtattt
    36361 tagatttagt ttaacgtatt cctccatcca aaaatcaagg tattgtttta ctcgagtatc
    36421 cgtacctaaa aagtattgtc ctgtttcgtc aatatctgat aaaacttttc gtaaagcagc
    36481 ttcggcctct ggtcgggtgt ctccgccaac tttctccact ttttttcttg agccatcatc
    36541 attgatatct
//
LOCUS       DAZABD010000022        36549 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000022 DAZABD010000000
VERSION     DAZABD010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36549
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.022"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(59..187)
                     /locus_tag="ACHALG_001181"
     CDS             complement(59..187)
                     /locus_tag="ACHALG_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFN2586605.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="ACHALG_001182"
     CDS             complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="ACHALG_001182"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFN2586606.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(1674..2729)
                     /locus_tag="ACHALG_001183"
     CDS             complement(1674..2729)
                     /locus_tag="ACHALG_001183"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFN2586607.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="ACHALG_001184"
     CDS             complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="ACHALG_001184"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFN2586608.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="ACHALG_001185"
     CDS             complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="ACHALG_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFN2586609.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="ACHALG_001186"
     CDS             complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="ACHALG_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFN2586610.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="ACHALG_001187"
     CDS             complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="ACHALG_001187"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFN2586611.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="ACHALG_001188"
     CDS             complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="ACHALG_001188"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HFN2586612.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            10491..12050
                     /locus_tag="ACHALG_001189"
     CDS             10491..12050
                     /locus_tag="ACHALG_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HFN2586613.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(12160..14091)
                     /locus_tag="ACHALG_001190"
     CDS             complement(12160..14091)
                     /locus_tag="ACHALG_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFN2586614.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(14109..15023)
                     /gene="pfkB"
                     /locus_tag="ACHALG_001191"
     CDS             complement(14109..15023)
                     /gene="pfkB"
                     /locus_tag="ACHALG_001191"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFN2586615.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(15020..15772)
                     /locus_tag="ACHALG_001192"
     CDS             complement(15020..15772)
                     /locus_tag="ACHALG_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFN2586616.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(15933..16142)
                     /locus_tag="ACHALG_001193"
     CDS             complement(15933..16142)
                     /locus_tag="ACHALG_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2586617.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(16153..17481)
                     /locus_tag="ACHALG_001194"
     CDS             complement(16153..17481)
                     /locus_tag="ACHALG_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2586618.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            17617..17772
                     /locus_tag="ACHALG_001195"
     CDS             17617..17772
                     /locus_tag="ACHALG_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPJ_0845 family protein"
                     /protein_id="HFN2586619.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(17846..18433)
                     /gene="yihA"
                     /locus_tag="ACHALG_001196"
     CDS             complement(17846..18433)
                     /gene="yihA"
                     /locus_tag="ACHALG_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFN2586620.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(18516..19766)
                     /gene="clpX"
                     /locus_tag="ACHALG_001197"
     CDS             complement(18516..19766)
                     /gene="clpX"
                     /locus_tag="ACHALG_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFN2586621.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(20013..21302)
                     /gene="tig"
                     /locus_tag="ACHALG_001198"
     CDS             complement(20013..21302)
                     /gene="tig"
                     /locus_tag="ACHALG_001198"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFN2586622.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(21456..22388)
                     /locus_tag="ACHALG_001199"
     CDS             complement(21456..22388)
                     /locus_tag="ACHALG_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2586623.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(22545..23204)
                     /locus_tag="ACHALG_001200"
     CDS             complement(22545..23204)
                     /locus_tag="ACHALG_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFN2586624.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(23207..24232)
                     /gene="sppA"
                     /locus_tag="ACHALG_001201"
     CDS             complement(23207..24232)
                     /gene="sppA"
                     /locus_tag="ACHALG_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFN2586625.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(24245..24535)
                     /locus_tag="ACHALG_001202"
     CDS             complement(24245..24535)
                     /locus_tag="ACHALG_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2586626.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            24944..26749
                     /gene="glmS"
                     /locus_tag="ACHALG_001203"
     CDS             24944..26749
                     /gene="glmS"
                     /locus_tag="ACHALG_001203"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFN2586627.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(26821..28176)
                     /gene="glmM"
                     /locus_tag="ACHALG_001204"
     CDS             complement(26821..28176)
                     /gene="glmM"
                     /locus_tag="ACHALG_001204"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFN2586628.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(28200..29369)
                     /locus_tag="ACHALG_001205"
     CDS             complement(28200..29369)
                     /locus_tag="ACHALG_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HFN2586629.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(29366..30253)
                     /gene="cdaA"
                     /locus_tag="ACHALG_001206"
     CDS             complement(29366..30253)
                     /gene="cdaA"
                     /locus_tag="ACHALG_001206"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HFN2586630.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(30442..34134)
                     /gene="nifJ"
                     /locus_tag="ACHALG_001207"
     CDS             complement(30442..34134)
                     /gene="nifJ"
                     /locus_tag="ACHALG_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFN2586631.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLHHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLNLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            34333..34854
                     /locus_tag="ACHALG_001208"
     CDS             34333..34854
                     /locus_tag="ACHALG_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HFN2586632.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            complement(35553..36110)
                     /locus_tag="ACHALG_001209"
     CDS             complement(35553..36110)
                     /locus_tag="ACHALG_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2586633.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(36289..>36549)
                     /locus_tag="ACHALG_001210"
     CDS             complement(36289..>36549)
                     /locus_tag="ACHALG_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2586634.1"
                     /translation="DLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDFI
                     LGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
BASE COUNT         9888 a         7926 c         6508 g        12227 t
ORIGIN      
        1 taaaaagact aagaaacaat aacaaaagtt aacacttctt agtctttctt tagcacgtct
       61 atttattaaa gattttaggg ccttttctca tgtttttacg atcattctta ggtgtttcca
      121 tttgtttgga tcggcctcca ccttgtttct ttttgatcaa ttctttcatt ttttcttttt
      181 ggtccatttt tattctcctt taacgaatga agcgactaat tcgatatgat gggtttgcgg
      241 gaaaagatct acaggctgaa ctttcttcac ggcataacct gcttctgcaa ataacttagc
      301 atctcttgca aaagttgccg gattacatga gatatagacg attcgttctg gtgacatttc
      361 gattgctgat tcaataaaat gatgatctaa tccttttcgt ggagggtcaa ctacgatcac
      421 agatggtttg atcccttttt cttgccattt tttcataact ttttctgctt ttccgacttc
      481 ataatgaaca ttttcgattt tattatttaa agcattgaac tgtgcatctt caattgcttc
      541 tggaataact tccatcccat acaccttatc cactctgtct gctaaagaca agccaatcgt
      601 acctattcct gaataagcgt cgatcacaat gtctttttta tctaactcgg caaactcgat
      661 ggctgtttgg tacagatact ctgcttgttg agtatttact tgataaaatg actttgctga
      721 gattcgatag gtttttccta atagctgatc ttcgatatag tctctgccat ataaaacttt
      781 ttctttgtct cctaaaatca catttgtttt ttcttcattg atattttgga tgaccgaaac
      841 cacttctggc aattcttcat gaatagcttc agcaatttcc ttggatttaa agaatttttc
      901 tttacgagta actaaaacga ccatcatttc atgggaataa tgaccacgac ggataatgat
      961 gtgacgaagc tgtccttcat ttttttcttc attatatcct cgtacttgga accgttgcaa
     1021 gatatctctg actactttga ttgcttgatc gatgacagga tcttggatat aaaaatcttc
     1081 gatagggact agatcatggc tattcttttt gtaaaaaccg gtggctaact gtccatcgat
     1141 acggcgaaca gggatctgtg ctttattacg gtagccgaat ggttctgcca ttccgaccgt
     1201 tggaagtaca ggaacttctg gcatcttagc aatcttcgcc attacatgct ctacttgttt
     1261 ttgtttgaag acaagctgtt gttcatactt caaatgggct aaaggtgcga tacctatgcg
     1321 aagtaatgca ttgttttcta caggctgacg atcaggtgat tgtgtaataa atgtttcgac
     1381 ttttccatat ccaaacttat tgcctgcttt tactactagt atttctactt cttctccagg
     1441 gagtgcattt tcgataaata atggataacc atcgattttt gctaccccca ttccttcata
     1501 actcaagtcg acgatttcga ctgtatatcg ttcgtttttc tttactattt gctgttctgt
     1561 catattttgc tcctttaagc ctatttttca atcctactga actctactat aaatcagcta
     1621 attacacaac taaaaccggc actgacctta ctacgttcag caccggcttt tgattaattc
     1681 ttttgattag gtgtttcgtc tgctattttt ccgtcaccgt cgatatcttc atccgttaaa
     1741 cgttcgactt cttttacgaa ttctttgctg acaacctcta attcttcttc atctgtacct
     1801 gtgattgctt catcaggaat ctgatcggta ttggcaaaga actcaatgtg ttgatgaaga
     1861 ttttggaaat gcatcggtgc ttccccgccg tattcaccat ctaagttgat cattactggt
     1921 cgattgatat catcttctgt ttcaacgaac aaactgcttg ttttggtata gattattctt
     1981 ggatcatcga catgtcgacc gccattcagc atgagtgctg caatatgcaa gatctcaaag
     2041 atatttgctg tcttcacaat gattaaagaa aacttcccat catccaattt ggcatctggc
     2101 gcgatctgtt cgaatcctcc aacagagttc gtcagcccta ggaaaaacat cgaagcattt
     2161 ccgatatatt ccccttcatc atacttcaag cgcattttga ttggctttac tcgagggagc
     2221 atctctgctc ctttcgcaag ataagcaaga taaccaaaaa tactctttag ttctgatgga
     2281 acttcataag tcaattctgt caaatagcca cctgcagcga tattgatgaa gtaactgtta
     2341 tcagaaaggc cgatgtccat ttttacggtt tggtgttttt ttatcacttc tgctgctgct
     2401 ttaatattat ctctaggaat ttttaatgct cgagcatagt cgttcgttgt accagctggg
     2461 atgatggcca ttttaggacg gtgttcaagc ggagcgattc cattaaccac ttcgttgatt
     2521 gtgccatcgc caccagctgc cactactaga tcaaaaccta gttctgcgac tctacatgct
     2581 tcattcttgg ctgaatctgg ttctggtgta gttgcatagg cgctggcttc aaatcctgct
     2641 tcttctaatg cctgtaggat atccgccaaa ttttttttca ataattcttt tcccgaggtg
     2701 gggttataaa ttacgcgtgc ttttttcatt ttcagcctct atcgtttcgc taattcatct
     2761 ttaagtagct tattcacgac accaggattt gcttgtcctt tagttgcttt catgatttga
     2821 ccgactagga agccaaccgc acgatctttt ccatttttga aatcatcgac agattgttgg
     2881 ttgttatcca aaatttcatt aatgattggc agtagtttgt tcggatcaga taattgcacc
     2941 caaccatttt tttcaactac ttcttgggct tctccgccat ttgtgatcaa ttcacggaag
     3001 acttttttgg cgatttttga gctgatcgtt ccatcggcga tcaaacgaat cattcctgct
     3061 aagttctcag gtgtcaattt tgtctcagct aattccagtt tctcactgtt caagtaagca
     3121 gaaacttcac ccatcaacca gttagatgct tgcttggcat ctgcaccatt ttccaaaaca
     3181 gcttcaaaaa aatcagacat ttcttttgta agtgtcaata ccattgcgtc atactctgga
     3241 agtccaaact cacggatata gcgttcgcga cgagaagctg gcatctcagg caaactagct
     3301 tttacttttt gaatccattc gtcatcgatc acgaatttcg ggatatcagg ctctgggaag
     3361 taacgataat cactagaccc ttctttgaca cgcatcaaaa ctgttttatt cgttgtttcg
     3421 tcaaaacggc gtgtttcttg ttggatttct cctcctgaaa gaagaacttt tgcttgacgt
     3481 ttttcttcaa aagctaaacc ttttttcacg aaagataaag agttcaagtt tttcaattct
     3541 gctttcgtac cgaactcttc ttgtccataa gggcgaagag aaatattggc atcacagcgc
     3601 atagagcctt cttccatttt cacatcgctg acttctgtaa attggataat tgatcgcaat
     3661 gcttctagat aagcgtatgc ttcttctggt gaacgcatat cagcttcaga aacgatttcg
     3721 atcaaaggcg tgccttgtcg gttcaaatca acgtaagaat aaccgccaat accatgcatg
     3781 ttttttccag catcttcttc taaatggaca cgttcgatcc ggattttttt ctttttacct
     3841 tctacttcga tttcgatcca tccgtcatga ccgattggtt gatcaaattg ggaaatttga
     3901 tatgcttttg gattatctgg gtaaaagtaa tttttccggt caaaatgagt atctttagag
     3961 atttcacagt ttaatgccaa agctgctttc atcccgaatt ccagcgcagc tttattcatt
     4021 actggcagca cgcctggata accccagtcg atcacgtttg tattgctgtt tggttctgca
     4081 ccaaaatgag ctggcgctgg agagaaaatt tttgagttgg tttttaactc gacgtggact
     4141 tctagtccaa tgactgtttc aaaattcatt cgtctttccc tcctaaaata gctggttgtt
     4201 gtttatggaa ttctgttgct tgttcaaacg catatgccgc ttgatacatc gttttttcat
     4261 caaaataatt accgatgatc tgcaatccaa ctggtaatcc ttctgataaa cctgccggta
     4321 cagacatacc tggaagtcca gccaagttga cagggatcgt taagatgtca ctcatgtaca
     4381 ttgtgattgg atcgttaatg ttctcaccga ttccgaatgc aactgttggt gaagatggcc
     4441 cgatgatcaa gtcataatct gcaaagactt tttcaaaatc tcgtttgatc aatgtacgga
     4501 cttgtcctgc ttttttgaag taagcgtcat aataacccgc gcttaaggag aatgtaccta
     4561 acatgatacg gcgttttact tcttcaccga atccttctga acgtgagttg acatatacat
     4621 catctaatga tttcacattt tcagagcgat aaccgtagcg gataccatcg aatcgttgca
     4681 aattggaact tgcttctgat gaggcaatga tataataaac agccacaccg tattttgaat
     4741 gaggcaagct gacttcttcc acaacagcac ctaatgaacg gaatgtttct gctgctttca
     4801 atacagcttc ttttacgcca ggggccacac cttcgcctaa gtattcttta ggtaaagcaa
     4861 tcttcattcc tttgatgtcg cctgtcagtc cctctgcaaa atcaggaact gaaacgcctg
     4921 aagaagtacc gtctttttcg tcataaccgc tgatagctgt caatgctaat gcattatctt
     4981 tcaccgtacg ggtcattggt ccgatttggt caagagatga agcaaaggca atcaaaccaa
     5041 aacgtgagac acggccataa gtaggtttca tccctacgat gccgttaaaa gctgctggtt
     5101 ggcggatact tcccccagta tcactaccta aagaaactgg aatttgtcca gctgcaactg
     5161 ctgctgcaga accaccagaa gagccaccag gtacttttgt tgggtcccaa gcattttttg
     5221 ttttcttgaa ataagaagtt tctgtacttc cacccatagc aaattcatcc atgtttagct
     5281 taccaacagg gatcatatct gcttgataga ttttatccat gaccgttgca tcataaattg
     5341 gttcaaaatt gtaaaggatc ttagatgcag cggttgtcaa gatatctttt gttacgatat
     5401 tatccttgat accaattgga ataccagcaa ggacgttgct ttcatcaatc cctttttcat
     5461 ctaaagcttt tgcctgttcc aatgcttttt cttcgttcaa tgtaatgaat gcctcgacct
     5521 caggctctgt ttctttgatt cgttcaaatg ttgtttttgt caaatcttgt acagtgattt
     5581 ctttcgatac aagcatatga tgcagttctt ctagagaacg attgtgtaat tctgtcatta
     5641 tgcgccagcc tccccattat cgatgattgc gggtactttg ataaagccgt cttctgtttc
     5701 cggcacattt ttcaataatt catcgcggtt ccatccagct tgcgcccgat cttgtcgcat
     5761 cacgtttact gtttcaacca cattcgacgt aaatggaaca ccttccgtat ctacttcttc
     5821 cagcatttca accatatcga tgattttacc taattggtcg gtaaaacttg caagttcttc
     5881 atctgcgaac gcaagttttg ctaatttggc aacgtgtttc acttgttctt cactaatcgc
     5941 catactaccc cttctttcta aacgagttaa aaactttgct ctctatttta acacaagagt
     6001 gccgtgtctg aaattgttta ttgaaatttg tctgaaaaaa ctcatttttc attcttctac
     6061 tgagcgactt ttagccaatg catctgccat ttgatcttct gtccaaactt cgattcctaa
     6121 ttcttgtgct ttggtcagct tgctgccagc atcttctcct gctaccacga tatccgtttt
     6181 tttcgatacg ctaccagtca ctttcccgcc aagattttcg atcgtctctt ttgcttcttc
     6241 acgagtaaat cgtgttagtt taccagtaag gactactgtt ttttctttaa aaggagactc
     6301 gacttcttgc aattgggcat ttcgaagccc tttgtattcg aaattgacgc ctgcttgttt
     6361 taattcattc atcaattcat ggacttcttc gttactgaaa taagtgacta cactgtcggc
     6421 aatgatatca ccgatcgtat caagcgcaat aatcgactca tagtcacttt tactcaatgt
     6481 ttctagatca ccgaaatgtt ctgctaaaat ttttgctgct ttcgctccga catgtcggat
     6541 tcctaacccg aaaatcaacc gctcgaccga attatctttg ctgttgtcga ttgctgtcaa
     6601 aatattattt gctgattttt cttttatttt gtccaacgtc aataactctt cttctgtcaa
     6661 tttgtaaaga tcagcaacat ctgctactag ttttttatca tacatctgtt ctagtacgcg
     6721 cggccccaac ccatcgatat tcatcgcatt tcttgaaaca aagtggttca aaccttcttt
     6781 catttgagca gggcatttag gattgatgca ccgtaaggct acttcttcat ccaaatggac
     6841 aagttcactt ccacaaactg gacaatgtgt aggcaattgg tattcttcgc tgtctttagg
     6901 tcgtttatct aacacgactt gcgaaacttc tgggatgatg tctcccgctt tataaatcag
     6961 aactgtatct ttcaagcgga tatccttcaa cttaatataa tcaccgttat gcaggctggc
     7021 acggcttact gtcgttcctg ctactcggac tggtgtcatg atagctgttg gcgttactac
     7081 ccctgttcga ccgactgtcc attcgatgtt ttcgattagc gtttcaactt cttctggcgg
     7141 aaacttgtaa gctgctgccc atctcggtgc tttgatagta aaaccaagtt gatcttgtag
     7201 cgagaattca ttcactttga tgacgattcc atcgatttca tagggcaaat ccacacgttt
     7261 gtcatgatat tcttcaatgt atgaccacac ttcatcaatc gaatgacata gacgtttttc
     7321 ccgattcgtg tggaatccaa ttttttctag ctcttctagc gcatcatact gggtttttgc
     7381 ttgcatagga ccgaaatcag ctactgtgta taaaaaagtg tccagatttc ttttcgctgt
     7441 gattttagaa tctaactgac gcaaagaacc agctgcggcg ttccgaggat tcgcaaagat
     7501 atcttttccc tctgcttctc tgtcttggtt caattggaca aatgaccgtt ttggcatgaa
     7561 acattcgcct cttacttcga tgttcattgg ttcttttaat ttgattggga ccgaccggac
     7621 agtttttagg ttttcagtga tgttttctcc tacagttcca tccccgcgag tcgcacctct
     7681 cacgaaattt ccatcttcat aacgcaaaga aacggaaagt ccatctattt ttagttcaca
     7741 gcaatagtca actacacgtc ccactgcttt ttgtacacgt tgatcaaaag ctatcagctc
     7801 ttctttgctg aaaacgtcgt tcaaactgta aagcggaata tcatgagtga ctttttcaaa
     7861 accttctaat acttttccgc ctactcgttg agtgggagaa tcagacgtga tcaaatcagg
     7921 atattgttcc tcgattgcta ctaattctgc gtattcttta tcataaacat agtcttccac
     7981 tgttggttta tcttctacgt agtattcgcg agaccattgg ttcagtcttg tacgaagttc
     8041 ttctgctcgt ttagcagctt cgtcaatcgt catttcatgt tccatcattt accctccact
     8101 actaagcttt ttcaattggc gcaaaggcag ccaaaagacg ttttactcct tgacttggga
     8161 aagccacatc tagttctaga tccttcgacg tcccgcttac tcggacgact gttcctagac
     8221 cccatttttt gtgtttgact ttatctccag gtttccagct gttattttcc cctccagtag
     8281 cacttttaga gggaacaacc gctgtctctg gctgtttata gctatatgct gtttttttag
     8341 cgaatacagg cgttttttta ggcgtaggac gcatgccttc gatttccagt aactcctgat
     8401 cgatttcttc tacaaaacga gaaggtcggt tatattgcgt ccgtccatat aatgttctgg
     8461 agaaagcatt tgttaaatac aacgcttctt ctgctcgcgt gatccccaca tatgcgagac
     8521 ggcgttcttc ctccagttcg ctttcttcca tcaatgctcg agataaagga aaaaccccct
     8581 cttctagacc aatcaggaat acaactggaa attctaatcc tttggctgca tgcaaagtca
     8641 tcaatgtcac ttgggaagca tcttcttcta aattgtcaat gtcggaaaca agtgccagat
     8701 catttaagaa gaccgttagt ttttcttctg gtgcatcagc gtcttcttca ttttgctgtt
     8761 cgaattgttt gtcgaattct tgtgtgaccg ttaaaaattc ctccaagttt tcgatgcgtg
     8821 cttgtgcttc taatgtattt tggattttta agtcttctag atatccgcta cgatccaaaa
     8881 cctctttcgt caattctgtc actgtcaagt atgggatcat ctgagtgact tcttggatca
     8941 tctctccaaa tgcacctaat tgctgtcctg cttttccacc gatatttgcc agatccacat
     9001 tttgcgctgc ttcaagcagt ggccattcat gaagagaagc aaaactgcgt agtttttcga
     9061 tagatcctgg tccgatgcct cgtttgggcg aattaacgat acgctcaaaa ctgattgaat
     9121 cttgaggatt taccagcaca ttcaaatacg ctaaaatatc tttgatttct ttacgatcgt
     9181 agaacttgtg ccctccaacc attttataag gaatatttgc tttcaacaga gtttcttcca
     9241 ttacacgaga ttgtgcgttc gtccggtaca agatggcaaa gtcgccgtaa ttccgatgat
     9301 tgcttcgaat ctcttcttgc atccggtcta cgataaaacg ggtttcgtct cgttcattat
     9361 ctgcacggta gtaagtgatc ttgtttcctt ctttgttttc tgtccacaag ttttttggtt
     9421 ttcgattgct attgttttca atgacttggt ttgctgcgct aaggatgttt ttcgtggagc
     9481 gatagttttg ttctagcaaa attactgcag catctggata atctttttca aaatccaaga
     9541 tgttctgcat atctgcaccg cgccagccgt aaatactttg atctgcatct cccacgacgc
     9601 acaggttcct aaatctacct gccaataaat tgactagcgt atactgtgcg tgatttgtat
     9661 cttggtactc atctacatga atataatgga atttgttttg ataatacgtg agtgaatcag
     9721 gatgttcttc aaataatcga atcgtattca tgatcaggtc gtcaaagtcc atacattgat
     9781 tatattgaag ctctttttgg taagcagcat aacattttgc cgcaatctct tcgtaataac
     9841 tgccttgcat ttttgcaaaa tcctgagggg tctgcaggct gttttttgct tgagagattg
     9901 ttcctaatat acttcgagga tcatattttt tcggatcaat gttcagttct tttagtatcc
     9961 gtttcatcaa ggtcagctgt tcagaagaat cgatgattgt aaagttccgg ttatacccga
    10021 taaaatcaac atctcttctt aaaatacgca cacacatcga gtgaaaagtc gatacccaga
    10081 catcttcgcc tccagatgcg agaatcgcat tgacacgttc tttcatttct tttgcagcct
    10141 tattggtaaa agtaatcgct aggatgttcc aaggattgac ttctttttcc tcaatcaaat
    10201 aggcaatgcg gtgtgtcagc actctcgtct ttccacttcc tgcacctgcc attagcagta
    10261 atggtccgtc agtgtgaaga acagcttctt tctgacgggg gttcatcccg tttaataatt
    10321 cagctttgga tgacattaac aacatccttt cttttatcaa tcttgttaat tatagcattt
    10381 tcttggcttt cattaaacta gctagttcaa aatcatttgt ttctttttat ggatttttcc
    10441 ttgtcaattc gttgatttcg cgctaaatta aaagaaagcg aggcgtttga atggaaaaaa
    10501 aaggaaaaat caagaaatta gataagactc agatatattt tattctaaaa ggaatatttg
    10561 ttggactgct gacaggaatc gtcgttagcc tgtttcgatt atctatcgaa aagctaagcg
    10621 atttagttcg aagtatctat gaaatgagtc gggaacagcc gatttactta attggcctag
    10681 ctatttgctg catccttgca gcatttttcg tgggttatct ggtcaaaaat gaaccggata
    10741 tcaagggaag cgggattcca caagtagagg gacagcttcg cggggaatta tcaatgaact
    10801 ggttctctgt tttatggaaa aagttcattg gcggtgtact ttcagttggt gcgggccttt
    10861 ttctaggtag agaaggacct tctatacagc ttggcgcatc cattggacaa ggagctggac
    10921 aattgtttcg gagtccatct tcagaagaaa agattttaat ttctagcggt gcaagtagcg
    10981 gacttgcagc agcattcaat gctcctatcg caggattact ctttgtttta gaagaaatcc
    11041 atcactcctt ttctccactt gtttggctga catcatttgc ttcagcaatc actgctaact
    11101 tcgtctctct ttactttttc ggcttgagac ctgttttata tctaggtgac cttccttctt
    11161 tgccgttgaa atattatggt tccttagtga tcttaggagt acttctaggc atcttaggtt
    11221 ttatttatca gaatgtcttg ctggctttgc caaaatttta caaccgattg cctttaccag
    11281 cttattttca cgggttcgtt cctttcttgc tgattttgcc aattggttat ttatggccta
    11341 gtactctagg cggaggcaat caaatcgttt tagcttttgg acatgcttcc ttgccaatca
    11401 ttatgattat cggtttgttt gttcttcgat tcgttttttc catgatttct tacggtgggg
    11461 gtcttccagg ggggattttc cttccgatat tgacattggg ggctttaatc ggtagctttt
    11521 atggaaacat tatggttgat ttcgggatgg atccaatcta catcaaggat tttgtcgtta
    11581 ttgccatggc tggttatttc acagcgattg gaaaagcacc gctgacagca attattttag
    11641 tgacagaaat ggttggctct cttaatcatc tgatgccttt aggattggct gctcttgttg
    11701 cttatatcgt caatgacctt ctcggcggta atccaattta cgaatcatta ttggaacgtc
    11761 tacttggaaa tactcagacc gcctcactaa aaggtgcaag gacaactttt gattttccag
    11821 taacggcaga gagttcttta gacggattaa tggtacgaga cttcaattgg cctaaaaata
    11881 tgctgctaat ctctatacgc agaggaaatc aggaaatctt gactcacggt gatacgatca
    11941 tgaatgtagg agatattttg gttatcttga ctgatgaagc aaacttgcca agaatcaagc
    12001 aggaaatcga ggctaaatct tctatcacaa atttgaagca atcattttaa cgaacatgga
    12061 aaaactattt tacaacaact attcatctac actacttgat tctatatgat tctataaaaa
    12121 agaagaggct gggaaaaccc agcctcttct tttttatttc tatgcttctg ctactgcagg
    12181 agcttttctc aataaaccat aaatcacacc agatacgact gctccgatag caataaacac
    12241 caaatacatg accgggtgac tcagtaagaa tacaacgaaa atcccgccgt gaggagccag
    12301 taatttgatt ccgaaagctc cgactaatcc gccagttaaa gcagaaccaa taataaaact
    12361 tggaatggca cgaatcgggt cagctgctgc aaatggaatc gatccttccg tgacaaacga
    12421 tagtcccatc acgatgttcg tcaaaccagc atcttgatct gttttgctga atttattctt
    12481 aaacaaacgt gtagcaacga aaattgccaa tggaggtacc attcctccag ccataacaga
    12541 agccatcacg acacttccgc cagttgcaac agtcgtagct aaagttgctg taccaaacac
    12601 ataagcggct ttgttgattg gaccgcctaa atctgccgcc atcattccag ctaataatgc
    12661 accaagtaaa gcagcattcg aaccactcat acttgtcaag aaaccgttca atgcatcatt
    12721 gattgctttc atagggacat tgaccagcaa catcaagaaa ccagtaatca gtaagccaaa
    12781 cactggataa aagaggatcg ttttaattcc atccaatgat tttggtaagt tcttgaataa
    12841 tttacgtaaa aatacgatga cataaccagc taagaatcca ccaatcagtg cacctaagaa
    12901 tcctgcaccg cctgtattag ctaatgcacc agcagcaaat ccaacgatca aaccagggcg
    12961 gtcaccgata cttgaagcaa taaatcctgc taataccggc agcatgaaac caaatgcagc
    13021 ttgtccgatt tggttgaacc agctagcagc ttgattgtaa ttccctagat ttgataattg
    13081 atcatgtggc acgccaataa attggtcgat catgaaagaa agcgcaatag cgattcctcc
    13141 accaataacg aaaggtaaca tgtgtgatac gccgttcatc aaatctttgt aaattctttg
    13201 tccgatagta ccatccgcat ttccttcttc tgaactgctt tcttttccat caccatggaa
    13261 tactggtgca gtaccactaa gggctagatt gatcaactct tctggtttac ggataccatc
    13321 actgacagga cgattgatca gttcttttcc gtcaaagcga ttcatctcta cttttttgtc
    13381 tgctgcaacg attacacctt ctgcacgggc gatgtcttca gcagtcaatc ggtttttgat
    13441 tccttctgaa ccgttcgttt cgactttgat ctccacaccc atttctttcg cttttttctt
    13501 caaagcatct tctgccatgt aagtgtgagc gatccctgtt ggacaagctg taacggcaac
    13561 aacgaatttt ctttctggat gagcagaagc ttctgcagct tgtgtttctt gttctgcttt
    13621 ttcttctgct tctttttgct cttcagctgt ttggaacaag tcttggactt cttctggtgt
    13681 atttgcttct tttaattttc ctacgaaatc tgcatcaatc agcaatcgag atagtgctgc
    13741 taatgcttgc aaatgtgtgt cgtttgcgcc ttctggagct gcgatcatga aaaacagata
    13801 agttggctgt ccgtctaagg cttcataatc tactcctttt tggctttttg cgaataaaac
    13861 tgttgcttct ttaactgcac tgtttttcgc gtgaggcatg gcgattccat caccaagacc
    13921 tgtagaagtt tgtgcttcgc gggctagaat tccttcttta tatgtttcga tatcggaaat
    13981 ccggccggca tcaaacattt tttggaccat ttcatcgatt gcgccttttt tatcagtcgc
    14041 ttgcaaatcc atgatcatcg cttctttgac cattaaatcc ttgatattca tgattatcct
    14101 ccttgaaact aatcaatttt tgtgatttca acttctggca gcaactcatc aatcaattcg
    14161 cgagctgcca agtcatcaga aaaagctgtt gcgcttccgc aagcaactcc ccatttaaag
    14221 gcttcgattg gatctttcgt acggctgtaa gccccaataa agccagcaat cattgaatcc
    14281 cctgcaccta ctgaattttt caatgggcga actaatacat tcgttttata agttccttgt
    14341 tccgtgaaga gcaaagcacc gtctccagcc attgagatca gcacattctg tgccccttca
    14401 gctagtaatt ttttcccata cggaatgatt tcttctacag attggaaggt tgtgttgtaa
    14461 agctcagcta attcgtgatt gtttggtttg atcaacaacg gtttcttaac aagtgcattt
    14521 tttaggtcat cacctgttgt atcaatcacg aaatctgcac ctttttctcg aatgatatca
    14581 atcagttctt gataaaatcc ttttcgcagg ctagctggcg cgcttcctga caatacgacg
    14641 atatctcctt gtcctactgt tcctaatgct tgcttcaact catcgatttc ttcatctgaa
    14701 atagatgggc ctaagccatt gatctcagtt tcagtgtctg attttagttt gatattgatt
    14761 cttgtatctt cttttacggt tgtgaacaaa gactggattt gttctttttc aagccacttc
    14821 gaaataaatt cgccagtgaa ccctcctaaa aaccctagtg ctgtagattg agaatcgatc
    14881 cgtttcaaaa tacgggaaac attgatccct tttcctccag gtaacttgaa gtcttccttc
    14941 attcggttca aatcgcctag ttttaaccca tctacgcgta cgatataatc aattgatgga
    15001 ttaagtgtta ctgtgtaaat cattgtattg cctccttaat tgttgtcttt tcacggaatt
    15061 cattaacgaa ttctaatgga caacgctcgg ttatgatcgt ggctgcttcc aaaggtgcaa
    15121 cagaggtgaa ggacaactgg ttgaatttgg tatgatcaat caatacaaat gtctcttcgg
    15181 aatttttcat ggctaaacgt ttaacagctg cctcttctgg atccggagtc gtgaatcctt
    15241 gttcaagatg ggcaccgttc attcccataa atgctttatt aaaatgaaaa ttttttagtt
    15301 gttctatcgc attagatcct aaaacagcat tagttgccag ctttatcgtt ccacctaaaa
    15361 taatggtagc aagctccaaa tcaactaaat ttgccgcatg ttgaacggaa tttgtcacta
    15421 ctgtgatctg ttttccttgc agaaaaggaa tcatttctaa cgtagtagag ccagcgtcta
    15481 aataaatgac atcttcgtca ttaatggttt cagctgccga tttggcgatt tgctgttttt
    15541 cgtgaacgtt tttgagtgat ttttcactca tattctgctc aaaattcaat aattgctctt
    15601 tcttagctcc gccatgaact ctttctagaa acccactatt ctccagctct tgtaaatcac
    15661 gtcttatcgt tgattcagaa gcattcaaaa ggtttacaag atcttgtgat ttcacgattt
    15721 tctgctgttc aagcaagtct aggatttttt gtctgcgttc ctctgtaagc attttcacga
    15781 tcacctcaat acaaataata gcatattttt tttctactag caatataaat cagtcaaaaa
    15841 cgttcataaa agttataaat cagtcaaaaa aaaaaataaa aaaccagtca gaaagactgg
    15901 ttttttattt ttttgtctat catcaagccg ttttatcgtt ttttattttc gcttcttgtt
    15961 tttttcatat aaacagaagc aggttttctt tccttatgaa gaagataata ataaagtccc
    16021 atcccgccaa acatgaccaa aaaaccgatg actgatcgaa taggaaaatt aggaacaaaa
    16081 aagagacaga cggataataa tgatgccgga accattattc gataatagag ttgtttatcc
    16141 attttcttca cctcatattt tttgtaaagt aatcaattgt tctaattctc ggtaatctaa
    16201 aaattgcaca cgcagatgct gctcaccttt cttgacatcg aattggatac ttcgccgctt
    16261 tttctttatt cgtttgaagg taacatcaga gagtctatgt ttgatttcct ttaacggtaa
    16321 ttctgacagg gattctgaag gaacatagat tcttaaggat aaaagatact cttgatattt
    16381 ttcatctgtt acctctactt ctagatattc cctaatataa taacaataag cttcgattcc
    16441 attatccaaa gcaacagtcg gatcgataac tttatagcac atcatgcgtg atcgattctc
    16501 gtcaacccaa ggaaaatacg cctgagcatt tagtgttccg cgaacacgcc tttctttata
    16561 atctccttgt tggatatcat aactttttgt tacaaaaaat gaagcattct ttttaggaac
    16621 aggttgcatt tgtgcattct gatactggac agattggatc gtatggtcag tgtaaatgta
    16681 ttcaacgaat ggcggtttca ataaaccgac accattgtcc caagcaagat gatcaaaccc
    16741 aagctggtcc ctaaaatagg attgaaacat ttgattgttt ggcttttgcc agttaaaggg
    16801 gtctcgataa agctggatac atatgacttc gttttctaat tccttcactt tttctacttg
    16861 ttggatgtcg agaaaaatcg gtttagggat gatttgatga taatagcgac tataatacat
    16921 ttcccaacac gaagtgaaac ataaaccttg gtaaaaaaga tcgtatcctg gaaattggtt
    16981 gcaatcgact actacttccc cgttttgttg tttcatttta ggtaaatgtt cgatttcatc
    17041 ctttttctca aacaatagcc gttgagagat ctgttttgta ttgtgataga taaactcgct
    17101 ataagctcgc atataggcga acaccccatg agcaatcatt tttttctgaa tgaagtctcg
    17161 aatcaaaaat tgcttttctt tgaaaacaga gatagacaat acatttttta tcaataattg
    17221 attatcttcc aagcgaaaac tacattcgtt ctcgtcattt cctacaacta accaactttc
    17281 gtctcttacc gatgctagca ttattttttc ttgaagcttt cgaagatcat acggatccgc
    17341 ttgttgtgtg aaagagcgga taaatgttgg ctggatcaac agacgtgtca aaatagccca
    17401 atcgcttatt tcctgttttt tttcttcttc atttaagtct gtgagctgga tagtcatcaa
    17461 taatttatct tcgccaatca ttttctcacc tctttcaaat ctttttataa tttaattata
    17521 accaatgata aagaaaaact aaaactttat gtcttagaaa ccaatttgga cgattacatg
    17581 atagactaag taaaaagaaa caaagaggtg tttacaatgg ggttgaaatt tgaacgtaca
    17641 gataatttag ataaactttt cgaatcattt gctgtcgatc cggaaaaaac agttgcacca
    17701 aaagaagaga tcgatcgatt tctaaaacca cagccacaaa atgaaaagaa aaaagacaag
    17761 aaacaaaagt aagttaagct caaaaaaaga cttaccagcc aaacttttat gaaagagttt
    17821 gactggtaag tcttttttat tctttttatg attcaattgc ttgttcgatt gctgcccacg
    17881 cttcttcttt tccctgtttt gtaacggaag aaaagagaat aaacgtgtca ttgcgatcaa
    17941 aatccaacgc ttttttgatt tcgctctcat gcttgttcca tttaccacga gggatcttat
    18001 ctgccttagt ggcaacaaca attacaggga tatcataata cttcaaaaat tgatacatct
    18061 ggatatcttc ttgagaaggc ttatgacgca aatctactaa agaaacgacc gcacgtagct
    18121 ggtctctagt cgttaaatat gtctcgatca tctttcccca ttttgctcgc tcagttttgg
    18181 atacttttgc atacccatat cccggaacat cgacaaaatg taatgcgttc tcgatcaaat
    18241 aaaaattcaa tgtctgggtc tttcctggct taccggaagt acgggctaaa ttctttcgat
    18301 caatcaatgt gttgataaaa gaagatttcc ctacatttga tcgtcctgcc agagctattt
    18361 ctggcaattg tgtatctgga tattgttttg gtgccactgc acttatgata atttcagctt
    18421 gatggacttt catggtctct ctccttaaca aggtgatgaa taaagcaagc ggctgtcgca
    18481 aaagccaaaa tgacttttac ggcagcctct tttcatcaac ccgctttttt ctctgaatct
    18541 ttatagatta ctgttggctc accagacaat tcagctgctt cttttgtgat aatcactttt
    18601 tcgatgtttt catttgaagg gatatcaaac atcacatcca tcatgatttc ttcaatgatg
    18661 gaacgaagtc ctcgagcacc ggtattgcgt tcgattgctt ttttcgcaat ggctctcaac
    18721 gcttcaggtt cgaattcaag ttcggtatta tctagagaaa gaagtttttg atattgtttg
    18781 actagcgcat ttttcggctc agtcaagatc cgtaccagat catctgttgt caatttctct
    18841 aaagctgcca taacaggtaa tcgtccgata aactcaggga tcaatccgaa ttttagtaaa
    18901 tcttcaggaa tgatatgctg catgacactt tcgtcttcag aaagtttttg gttttgtgta
    18961 ccaaatccaa tcgtcttttc gcccatacga ttcttcacaa tcgtttcgat gccatcaaat
    19021 gcaccaccta cgatgaacaa tatgttcgtc gtatcaatct gaataaattc ttgatgtgga
    19081 tgtttacgtc cgccctgagg tggcacactt gcaacagttc cttctagaat tttcagcaag
    19141 gcttgttgga caccttcgcc ggaaacgtca cgagtaatag atacattttc gcttttacgc
    19201 gcaattttat cgatctcatc aatatagatg atcccttttt ctgcaagttc cacattgtaa
    19261 tcagcagatt gaagcaattt aagcaaaatg ttttctacat cttcccccac ataaccagct
    19321 tctgtcaaac ttgtcgcatc tgcgattgca aaaggtacat tcaatgtttt tgctaatgtt
    19381 tgtgctaaaa atgttttacc agaccctgta ggtccgatca agcagatatt gcttttttgc
    19441 agttcaacat catctgtgtt ttcttgtgct tgctgattga cacgtttata gtgattgtat
    19501 acggcaacag ctaacgagcg ttttgcgcga tcttgtccaa tcacgtagtt attcaatact
    19561 tcaagaattt ctagtggttt agggacttca gtgaattcgc gtacagcttc ttcataaaat
    19621 tcttcatcaa tgatttcttt acataggtcg atacattcat tacagatgta gacccctgga
    19681 ccagctacga tctttttcac ttcttcttgg gttttcccgc aaaaagaaca gcgtacggtc
    19741 tcattgctac ttggattgtc atacatggtc ttcacccctt aacttgcaaa aagtatgcaa
    19801 gaaactatca aagactttac agtcttttac tatcataaca tagtttagta aaaagaaaaa
    19861 gtgtttaaca aaggatcaac tgacctatac ctttgcattt tttactctta acaaaaaata
    19921 aggaactgtg acaaaatcgt gtcacagtcc cttatttcga ataaacaatt ttctagaagt
    19981 agcttcttca gaaatgattt attacttgat ttctattctt tcgcttctgt cgctgtacct
    20041 gtgatcaatt cgattgcttt tttcattgtc acatcatgtt ccagcatatc ttctgtcaac
    20101 acgcgtttga tttgttcgat tggcatgttg tatgtttctg ataattcttg gatttctgca
    20161 ttgatttctt cttctgttgc ttgaagattt tcagcagctg caattgcttc gatcacaaga
    20221 tttgttcttg tgcgcatttc tgcttcacct tcgaattgtt tatgcaagtc ttcttctgta
    20281 gaaccagtta attgatagta catttcagga gagatacctt gacgttgcat gttgttcaag
    20341 aattcgtcca ttgaacgatg tacttcatca tgaaccatca cgtgtggcaa ttcaacgatt
    20401 tctgcgtttt caacagccat acggatcgct gcttcatctt ttgcatcgtc cgctgctttt
    20461 tcttttgttt ctactaattc tttgcgatat ttttctttca attcgtctaa tgttgatact
    20521 tcgtcatcaa catcttttgc gaattcgtca tctaattcag gcacttcttt tgcttttact
    20581 tcatggattg tcactttgaa gactgcttct tttccagcaa gatcttctgc ttgataatct
    20641 tctgggaaag ttacatttac atctagactt tcacctgatt tatgaccaac taattgatct
    20701 tcaaaacctg gaatgaatga gcctgaacct aattcaagtg agtaattttc gccttttccg
    20761 ccatcaaacg cttcgccatc tacaaagcct tcgaagtcga ttactactgt atcgccattt
    20821 tcagctgcag catcttcttt gataactaat tcagcttgtg cttcttgttc acgtttgata
    20881 cgtgcatcca cgtcagcatc tgttacttca cggtcttgtt tttcaacaac tagatcttta
    20941 tattgtccta attttacttc tggtttcact gttacttcag cagtgattac ccagtcttga
    21001 cctttttcca tgctttcaac atcgattttt ggttgagaaa caggatcgat gccagcttct
    21061 ttgacagcag cttcataagc ttctggtaat acagcattca atgcatcttc atataaagct
    21121 tcttcaccgt acatacggtt gaatacttga cgagaaactt tccctttacg gaatccaggt
    21181 acgttcaagc tttttttcac tttattaaac gcagttgtta atcctttttg gatttcgtct
    21241 tgcgcaatcg taaattttaa aacaccatca ttcgtgcctt ttttttcaaa tgttgcagtc
    21301 atttaattcc ctccgagttt agaaattcat tttatacatc aaaaacgttt catgcatacc
    21361 tttaaatagt acactaaccc tttcaaattg taaacatatt gaatggatag aatccttgtt
    21421 tttcataaca tttttatctt tttcactgat aaaatctaat ggaagaagat catttgctgc
    21481 aaaatttttt ctcttttttt taaaaaatct tgtttttcat cttctgtacc ttcatacatc
    21541 gtttttcctg tacacgcctc tacagtcagt tgaaaccagc gcttcgggtc ttcgatgtat
    21601 gtatcataga caggataaac aattgcagat tgcaacgtga attcttcttt taacacggaa
    21661 ctaagcagcg catcttcatt cccgtaacga tctgaaaata cacataattg ctggtaactt
    21721 gcctgctgct gcggctttgg cagatctttt ggtatgactt tgactaactt ttcttcaatc
    21781 gtcaaataga aaacaggatg gcattcgttc aattgcctaa gtgtctccag taattttgcc
    21841 cttgctaaaa tatggacctc tggcgataca ataagtttac tcgctgtttg gataaactca
    21901 tgataaggaa gctgttcgat ctcatcaata gacgcaagct gacgtgcagg ttccaacgaa
    21961 ggaagttcct ccgatttatt gtggagtgcc ttgatcttct gcctctccat ttgtccggat
    22021 aattcttctg caaattggat tttttcaatg acccgctgct cgaaagcttc ataaaaatct
    22081 gtacttgcta tcaattttct agcatataaa aaatcttgtg tactgattaa caagtcaaaa
    22141 taaaattcag caaactcctc attttgaaga tactgttttt catgcaagag tgcttgcttc
    22201 aatgcttctt ttttctcgtc caactcgtaa agacaaaaaa caagcaggct attagcctca
    22261 aagctttctt tcaactcgta agctcgttga aataattctt ttgcctgctg atattgctgg
    22321 tttgctagtg cttgttgtgc cactcgcatt aaattctcat atttttctgg aaaatcaatc
    22381 ggcttcacgc agtgtcctcc tcttgctcat attcagctca tgttcaatag tagcaaaaca
    22441 aaaaaagcat gacaattaaa aataactgaa gcaccctcta aaagctagat tttagatctg
    22501 acttttagaa ggtgctttca actgttactg ctttttattg ctcatcagct gttgacttcc
    22561 tggttgaacg ctttgatcag attgatcgta acaacacttg tattagtgaa aaaatcgccc
    22621 agatgttgct ttttctgatt aaagactgct ggaagatacc ctaacatcag caaaggaaat
    22681 tttagaatga accggcaagc accttctcta actaaaacgg ttgaccagct aagttcttct
    22741 tcatcaaagc tgatgactcg aataccaaag atcattttcc caatcgtttg gccatgattg
    22801 aatttcgtca ataaaataaa atacgctaga taaataagta aggctgtgaa tccgtagaga
    22861 ctcagataag aatctgaaaa attaagccaa cctagatttt tggcaatacc aagtgttaaa
    22921 cgcgtaatag ctccgataca caaaagatcg accaagaagg caaacattcg gataaaaaaa
    22981 cctgcataga aatacttagg aaaatcattt gcttttggcg gttcatttct tgtgtccgta
    23041 tactttttcc atttacgttg tttctttttg atttcttcgg gcgtcaaagg ctctctttca
    23101 aaagagctca gtaccttttg ggaaaagttg gagtcaggtc ctacagcagg ctgctgttgc
    23161 ttatcttttt cttgttcctt attttcattt gaaagctctg acatgcttac tcacctccgt
    23221 aataatacat tgcttttggt gattccgctg tccctagatt ctcaagaacg gataagatct
    23281 gactagtctc agaaggcttc aatccctgga attctgctag tttacttcct aaccatgtac
    23341 tagcaaaccc agatggcgtg gaagaatatt ctactagttc cgcatcttca agctgttgtt
    23401 cctttcgtat agcttttaaa gcatcttcag gaaatcccaa ttcatcgact aaacctactt
    23461 cttttgcttg gacaccatca taaatccttc catcagcaat ttttttgact tcttcttcag
    23521 atttattccg tccctcgctg acgattgcta caaaacgact atatgcgctg tcgatatatg
    23581 cttgaagcac cgcttcttct tcttttgtct ctggtcgcgt tcctgaaccc atatctttca
    23641 acgctccgct tttaactgtt gtatcttcca cacccagttt ttctagaagt cctgaataat
    23701 tcaatcctga catgatcaca ccaattgagc cggtcactgt ttcttctgtt gcaaagattt
    23761 tatctgcttg agcagaaata tagtagccac cgctagctgc catgtttttc atgctgacat
    23821 acattgggat atcatgttct ttacgaatcg tatccaattt cttagcgatt tccgcacttt
    23881 cgtagacacc gccaccagga gaatttactt ctaaaaagat acctttgact gttggatctt
    23941 cttcaatcgc cttcagttgt tccataaaat ttttatgatt gtaaccttca gaagcaaata
    24001 aacctgaaga ccctcccgca gcaatcgttc catctactga taattttacg attttttgcg
    24061 tactgtctcc ctcttccaac acatttggag ctagttcttc atccccatat aaccaactgt
    24121 tcaatccctc aatttgatcg ttttcctgag gcttagtcaa actagctgaa actaacgaaa
    24181 ccacaaataa tcctgcagca atcagaactg ctgcccaacg tcttttattc attctttctc
    24241 ctttctaata caagtttttt atgacgatca cagtttctga ctgctcagaa gtgtcgaacc
    24301 gctctgcttt ttcttcccgc gcatacccgg aaaacgactc ttttgttaat tcatcttcta
    24361 ccgtaaactc acgagcttga gcaacttcca aggcatcagg tgcgatttct tggatgctat
    24421 cccagccagt atctaaaaat attttcgtat catctgatac accagctgat gcttcgaatt
    24481 tggacaattg tttttttaac attcccagtg tcatgatctc aatttttgaa tccattttta
    24541 ctcctcctct atccgattac tttctattgt accgaactcc tgaaaaagat gatagataaa
    24601 aacccattat tttttgacat aaaaaggaca tttgacaaag aatttgatat ataccaattt
    24661 attttttatt ctttttttgt tgacaagaac attcgcaaaa ggttatgatt ttaaatgttt
    24721 ccagataaaa aacaaaaagg aggatagtta taccttttag gatagcgcca ggtctgtgta
    24781 aacagatgac gaggagaggg tttatcgaaa gattcggcgg atggccctag ggactgcact
    24841 ctacagaaaa tccacaaaaa atgtctgcaa agataaaaca aaaggatttt cctgcagctg
    24901 gaagcattca tacgaattca ttatagaaag taggatgttt tttatgtgcg gaattgttgg
    24961 aatgattgga ttaaaaaatg taacaccagg attgattgac ggtctagaaa aattagaata
    25021 ccgtggttat gattcagctg gtatctttgt ctcagatggc acaactgatt atctggtcaa
    25081 agcacaaggc agaattcaaa atttgaaaaa caaaataaat attgatacta caggaaatat
    25141 aggtattggt catacacgat gggcaactca cgggcaacct tctgaagaaa acgcccatcc
    25201 acatacttcc caaagtggac gcttcgtgct cgttcacaac ggcgtaatcg aaaactttga
    25261 agaacttaaa attgcttatt tagcaaatga ccactttatt ggtgagaccg atactgagat
    25321 tatcgctcat ctaattgaaa ctttcgcgaa ggatactaca acgcaagagg cgtttttaaa
    25381 agctttgcgg gtcatcaaag gttcttacgc tttcgctttg attgatcgga cagcacctga
    25441 tgttatttac gtcgctaaaa ataaaagtcc actattaatc ggactcggcg atggattcaa
    25501 tgtgattgcc agcgatgcaa tggctatgct tgctcatacg aaggaattcg ttgagattga
    25561 agatgaagag atggttaccg tcacatcaga aaaagttatt attcaaaatt ttgcaggtga
    25621 catcgtagaa cgttcttcat tcgaagcaca agtagatgct tcagatatcg agaaaggtac
    25681 ctatcctttt tatatgctga aagaaatcga cgaacagcct attatcatgc gtcgtatcgc
    25741 tcaaaaatac gtaacagaag acaatcgtgt ctctcttgat caaaagctgg tcgatacatt
    25801 gtctgatagc gatcgaatct atatcgttgc ttgcggaact agttaccatg cggggttagc
    25861 aggtaaacag actttggaaa aattgacaca gattcctgtt gaggttcatt tagccagtga
    25921 atttggctat aacacacctc ttctttcaaa aaaaccattc tttattttct tgagtcaaag
    25981 tggcgaaaca gcagacagcc gccaagtgct ggtaaaaatc aaccgtctcg gctacccttc
    26041 acttacgatt acaaatgttg ctggttctac tctttcgcgg gaagcatcct ttacactgct
    26101 attgcatgct ggacccgaaa tcgcagtagc atcaacgaaa gcttatactg cacaaatagc
    26161 tgtactaact ttattagcca aagctatcgg tgataaaaaa gaatttgaaa cttctcttgt
    26221 attcgatatc actcatgaat tgagtttagt tgctaatgcg atggaatcgg tcatcgccca
    26281 aaaagattat ttagaagagt tggcagaagt atatctcagc gatacaagaa atgcctttta
    26341 cattggacgt ggtgctgact acaacgtttc tcttgaagct gctttgaaat tgaaagaaat
    26401 cagttatatc caggcagaag gttttgccgc tggcgaattg aaacatggaa cgattgcttt
    26461 gatcgaagaa ggaacaccag tgatcggaat catcagtgaa gaagtgactg gtgcgcatac
    26521 tcgaggaaac ctgaaagaag tcgaaagccg tggcgctaaa acactagtta ttgtctcaga
    26581 gggattagaa aaggaaagtg accagctggt gctaccaact gtccaccctt atctaactac
    26641 tctaaccaca gtcgtaccga ctcaattact tgcatattac gcaacgctat tacggggatt
    26701 cgacgttgac aaaccacgta atttagcgaa atcagtgaca gtagaataat aaaagaacaa
    26761 ggaccagtgt cagacatctg acactggtcc ttgttctttt attttttatg aaaatatctc
    26821 ttaatccagt ccgatttctt ctttgactac tgccgcgatt ttatcgacat agtaattaac
    26881 tttctcatca gtaggcgctt ctgccatcac tcgaagtaat ggttctgtac cggaaggacg
    26941 aactaggatt cttccttctg atcccatttc agcttctgcc tcttcaatca cttttttgat
    27001 tgccggaaca tccatcgcac cgtttttatc acttacgcgg atattcacta atttttgcgg
    27061 atagattgtt acctctgctg caagctctga taatttctta cctgtctgtt tcatgatatt
    27121 cagtaactga attcctgaaa gcatcccatc tccagttgtg ttaaaatcta agaaaatcat
    27181 gtgcccagac tgctcgccgc caaagttata atcgtttttg cgcatttctt ctacaacgta
    27241 acgatcacca acttgtgtaa cgacatcttt caaaccgatt ccttccaccg ctttgtggaa
    27301 tcctaagtta ctcatgacag tcgtaacgat cgtatctttt ttcaatcgat ttttttctgc
    27361 caagtattta gcacagataa acatgatttt atcaccgtcg acaatgtttc caagttcatc
    27421 caccgcaata atacgatcgc catcgccatc gaaagctagt ccagcatctg ctcctttttc
    27481 aacgaccatc tcagctaaac gttctggatg ggtagagcct acgccgtcat tgatatttaa
    27541 cccgtctgga tttgtgccca ttgtataaaa atctgtttca agatcggcaa acaaacgatt
    27601 aacacttgtt gaagttgctc cgtttgctgc atcgacacaa atggtcaaac cggaaaggtc
    27661 accgttgatc gtttgtacaa ggaattgaga atatttcaat aagccttctg gaaattcttc
    27721 tacagttccc aatccttcag ctgatggacg aggcaattca tccacttcag catctaataa
    27781 tgcttcaatt tctagctctt ggtcatctac tagtttaaaa ccatctgcac cgaaaaattt
    27841 gattccatta tcttgtgctg gattatgaga agcagaaatc atgacccctg cgcttgcttt
    27901 ttgcaaacgt gtcagataag caacacctgg tgtagagatc acacctagct ggaacacttc
    27961 gatacccacc gataaaagtc ctgaaatcag cgcttcttct aacatttgac ctgagattct
    28021 tgtatcccgc cctactaaca cgcgtgggcg ttttccttct tcttcatgct ggcttaggac
    28081 ataacctccg caccgtccca gcttaaatgc aagttctggc gtaagttcct tgttggcttc
    28141 accgcgtacg ccgtctgttc caaaatattt acccatagtt gtaaaattac tcctttaact
    28201 taattttctg aggaatttgt tctgctcatt tgtgttgatt cgcttgtttc tgatgtttct
    28261 tctgtacttg tggtcgagct gcttgtccca gattccgaac tggaagaaat ggttgttgtc
    28321 gatggttccg ttcctgaatc agcagatgaa atacttctac tcgtacttgt attttcttgt
    28381 gtccgactgc tcgtcgaagc agagctactg cttgacgttt gcaacacggg tgtaatctcc
    28441 acactaactg attcgggctt gatacttaac ccctcttcta ctggtatttc aattttacga
    28501 gttgttcgat gattgatacc tgagacattg atcggtacag caatactgtc accgatcgat
    28561 gcaagcaaat cgctagagcc gcttacttct gcttccagtt ttgaaaaact gaaatcatag
    28621 ctttctaccc cggcagctgg tgttccttgc tgtatcccat aaagtcgtac cgacttgctt
    28681 ggcttgatcg aatcagctct cactgttact tgcactggat cagagacgat acttaatact
    28741 tcccctgctg cattcagcgc ctgtattggg acctcactat taatcccatc atctgtgacc
    28801 atcgaagcgt ccacatcagc tactactcgt ttgatttctg ccaatgtttt gtcacctgta
    28861 gtgatagaga cttttttagg actaacggaa gtatcatcaa gttcatatcc agatggtgtg
    28921 aacgttgaag gaatcaacgt ttccacatcg aaatctttta ttaccttttt ctcaaccgtt
    28981 actgtaatag tcgatggttc gatctttgct gtgacagcac tgctcaaatt ttgaattttt
    29041 agcggaactt catgtgttcc ttcgcccagc ttagtgatat ctgctgtgac gcggaacatc
    29101 cgtgtatctt cgtttgcctc tgcattcaac tgaactcggt ttgcactgga aagtttgaca
    29161 gtcactgcat tttcatatcc atggatatag tagcgctttg aatcataaga aatcttgatc
    29221 ggcacccccg taactgtttg attatacgct tcatttccag aaagcgtatt tggaacggac
    29281 tggccatttg cattgaagta cagcgcaata ctgaacagca atgcaataaa actgtaaaga
    29341 atgcttgtcc gtttctcttt tgagatcatt tgcgatcccc tcccttagtc ataccgtcta
    29401 ggaaagattg aatcacgttt ttcttgtttt ttgttttctc ttcagcaatt agttctctac
    29461 gcaagattgc caaatattct tgctgggata agccagccat cagttcatta tctaacgtga
    29521 tgctgacgtc cccggtttct tcagagacaa cgatggtaat cgcgtcactg acttcgctta
    29581 taccgacagc tgcccggtgt cgagtcccga attctttagg gatcaacata ctttctgata
    29641 gaggaagata ggcgcttgct actgcgatct ttccttcttt tacaataact gcaccgtcat
    29701 gcagcggtgt attaggaata aagatattga tcagcagctc cccagtgata tctgcatcta
    29761 aagcaatccc tgtctcaata tactcatcta atccagtatg acgttcgata gtgatcaacg
    29821 caccgatttt tcgcttggac atatattgga tggctttgtc aaaagacaag atcatcttct
    29881 catcttcctg ctgttcgctt tttgattgtt tgaaaagcga actgcgtcca agatgttcca
    29941 gtcctctccg gatttccggc tgaaaaatca cgatagcagc tatcaccccg tacgtaatga
    30001 cttggttcat cagccaagac aaggtgtgca gaccaatgat ttccgctacg attcggataa
    30061 caataaaaat agctacacct ttcagaagct gaatcgcttt tgtgcctcgc agcagttgga
    30121 tcagtttgta tacaagatac cagacaacca gaatatcaag tatgttcacc aaaaaattca
    30181 gagagaacaa gtcaccggaa aacatttgct gccaatagtc caaattaaaa agttccccga
    30241 tttgaaatga cataaaaagc ctctcttctc ttttgagatt tcctctctag tatatcatat
    30301 gttttaattt tagaggctga tatgctcgta tttttcttga gaaaaacgca taattttcaa
    30361 gaaaattgca agtatgcgtt ttatttttct ctgcaataaa tatttttaaa cttcagagaa
    30421 ttattcatta tattttttct gttatttttc ctttttttct tttgtagctt gttccatttt
    30481 tttacgtcgt tctgctctgg cagctcttct agcagctctt ctttcttctg cttcaaggtc
    30541 cgtcgttttt tcttttctag tcttaacggg tacatcgata gattcctctg gttcaagtcg
    30601 tttcttgatt ttttctaatt ctcccgtcag tgcagcatag ttgtaaaaac gtgttttagc
    30661 gtcgtggaca gtcttttcaa ataaagcttt tgcaagattt ggatttgcgt tatctagcga
    30721 ggaaaagcga acttgtttcc tcatgaaatc aggcatagca gagaaatcag gttttttgta
    30781 gtccaaagtc attgggttct tgtttttttc gaccattgta gggttatatc gatataatga
    30841 ccaataacca gaagaaacag cttcttttgc ttctttaagc gattctttca ttccgccaag
    30901 caagccatgt gtgatacaag gcgtataggc aataatcaga gaaggaccag gatacttttc
    30961 tgcttcttca aatgccttga tcgtttgcat ttgattggca cctgaagcaa tttgcgccac
    31021 ataaacattt ccatatgtca tcgccatcat tcctagatct ttctttgaag catactttcc
    31081 actagccgaa aatttagcga ttgccgaagc tggtgttgct tttgaggttt gaccgccagt
    31141 attggaatag acttcgttat ccaaaacaag gatattcacg tctgctccgc tggcaagtac
    31201 atgatcgatc ccgccaaaac caatgtcgta agcccatccg tctccaccaa tcatccattg
    31261 gctgtttttt acgaatagat cctttctctt gtatatatcc tccagttcag ggaaaattga
    31321 tcgttcgctt tcgatcgcag cttgcagttt tgctgctctt tgctgcgttc cttcactctc
    31381 aaacatgtgt actaaccagt cttccatcaa ttctcgtagt tcttctgata cttttggcaa
    31441 tacttctttg attgaacttg ccaaagcctc gcgacgagtc tgtgccgcta gaagcatacc
    31501 ataaccaaat tcagcattat cttccaataa agaatttgac catgcaggcc cttgaccaca
    31561 ttcattggtc gtataaggag aagcaggaga agcaccgccc caaatagaag aacagcctgt
    31621 cgcattcgcg atcatcattc gatcaccgaa catttgtgtc aataatttta cataaggtgt
    31681 ttcgccacag cctgagcaag cgcccgagaa ttcaagaagc ggttgattga attgtgtaga
    31741 caagacagta ttctttcctt tgactggatt tgctttttgt cttaatgtca ttgcaaaagc
    31801 ccaattcaca gcttgttctt tctgttcttc ataaggcttc atcactaaag ctttttcttt
    31861 tgcgggacaa gcctctacac acagaccaca acctgtacag tcttccactg aaacttggat
    31921 acggtatttt acgccgtctg cgcctcgcat ttctctcacg ataaatcctt ctggcgcttc
    31981 ttccatttcc tcttcatctg ctaaaaatgg acggatggca gcatgaggac aaacaaaggc
    32041 acattcatta cacatcgtac agcgatcact aatccactca ggtacttcta aagcgacgcc
    32101 ccgcttttcc atagaagccg ttcctaaagg catttcgcca gttgtcattc catttgctac
    32161 aagtgtacca acagagagtt gatttccttc ctgtgcattc actggttcaa gtatttcttg
    32221 tatataacca gtgttccttt tattgatttt ttcaggcatc tccaacgttc tccaatggtc
    32281 tgggatcgcg acttgatgaa gcagttcggt cgtccgatcg attgcttgta tgtttttttc
    32341 tacgatagcc attgatttat gtccatagct tttcagggct tcttctttta gcagcggaag
    32401 cacctcatca aaaggaatga tgtcagctaa tttaaaaaaa gccgtttcca ttgccgtatt
    32461 gatccggcgt cccaaaccga cttcttgggc caatctcatg gcattgatcg tatagaatct
    32521 aatgttattt tcagccaagt aacgttttaa ttttaagggc agatgcgttt ctaattgttc
    32581 gtcgctccaa agagtattca ataaaaaaat gccacctggc ttcaatcctt tgaccaagtc
    32641 atagctatgc aggtaagctg gtgtgtgaca ggcaacaagg tccgcatgct cgacaagata
    32701 cgctgaacgt atcggcgtat ctccaaaacg aagatgagag acagtcagac cgccagattt
    32761 tttcgaatca taataaaaat agccttgcgc atatttatcg gtgtgatctc caataatctt
    32821 gatagctgat ttatttgctc caactgttcc gtcagatcca aatccccaaa acttggcctg
    32881 gaatgtttgc ggttctgtca aattcagact ttcctttggt tccaaagatt gataagtcac
    32941 atcatccacg atccctatgg taaaccgttt tttgcgtatg cttgggtctt ttttcaattc
    33001 atcaaaaact gctgagattt ggtcgggagt cacatcttta gaccccaatc cgtagcgacc
    33061 accaataaca gccgggcgaa gttcagaatc atataacgca ctttgaacgt ctaataagag
    33121 aggttctcca cctgcccctg gttctttact tcgatcaaga acagcaattg tttttactgt
    33181 atgatgcaac ttttgcacga atgtttccac tggaaaagga cgataaagat ggatattcaa
    33241 aaaacctgtt ttccgtccgt ttgcattcaa gtaatcaatc gtttgttcaa tcgtctgtgc
    33301 aactgatccc atagaaacaa tgacttcttc cgcatcgtct gctccgtaat aagtcaccaa
    33361 gtcataagaa gttcctcgta atcggttgat ctctttcata tatttttgaa cgatttcagg
    33421 taatttatca taataggggt tgatcgtttc ccgttgttga aaatggatgt ctggattctg
    33481 gttcgtacca ctaacagtcg gatgattggg attcatccct cgtttacgaa atgaagctaa
    33541 tttctcctga ttgatcagag gagctaattc ttcatattcc aaaacttcga ttttttgaat
    33601 ctcgtgactc gtacggaatc catcaaagaa attcaaaaaa gggacttctc cttcaataga
    33661 agcaagatgg gcaacaggtg ctaaatccat cacttcttgt acactgcttt cagctaacat
    33721 agcaaatcct gtttgtcttg cagccatcac gtctccgtga tctccgaaaa tattcaacgc
    33781 atttgtcgtc actgcccgac tagcaacgtg gaaaacactt ggcagtagtt ccccagcaat
    33841 cttgtacata ttcggtatca tcaaaagcag cccttgtgaa gcggtatatg tagttgtcag
    33901 tgcaccagtt ttcaatgatc catggaccac accagctgct cctgcttcgg attgcatttc
    33961 tactactttt actggttgac cgaatacgtt ttcttttcct gctgctgacc attgatctac
    34021 taattcagcc atggtcgaac tgggtgttat tggatatatt gcagcaatct cggtaaatgc
    34081 ataagaaata tatgcggctg ccgtattgcc atccattgtt ttcatttttc gcatgattca
    34141 tcatccttgt tcatattctc tcttattatc tgttccatta taacgtaaaa catgccattt
    34201 ttttctttta tttataaaaa tattttacat gtatctattt gaattggtca tctaattttt
    34261 agacaaacag tctaaatcgt tgcctttttt tgtcagacat gtataaacta taagcgaaag
    34321 aggtgtttta acatggatat atcagtgatt gatgcaacga aaacaaatac acaaaaagga
    34381 atcctttatg gttcatcgaa tgctcctaaa aaaatggtcg aattcatcaa cttagcttgt
    34441 ccgtattgcc gacaatggtt tgaagaatct tatgagctat tagaagaagc agtccaaagc
    34501 gggcaattgc agcgagtgat caaattgttc gataaagaaa aagaaagctt acagcgaggg
    34561 aacgtgatgc accggtatct cacaatcagc gatggccaaa aagcgatcaa agagatcaaa
    34621 caaatattcg atactcaaga cgagtggaaa catctttctc tccaagaagt agcggatttt
    34681 gcagtagata aactaaagct gacagaacaa aaagacgaac agctgagcca agctgtcatc
    34741 aacgaagctg agcaagcaca tattcgattt gttccaactg tcatcttggg taaagaaatc
    34801 tttgatgaat ctatttcaat agaggaatta aaagaattga tccaaacaaa ataaaaaaat
    34861 aaggagcgtg ggacaaaagt aaagaacact tttgtctcac gctctaaaca cgtataaacg
    34921 gcggaagcag aagcaactcc ttcttatttc ttggagttaa acgcttctgt cccagcctcc
    34981 ttattccgaa taaatggtgt ccaaaagctt gcatctgcca gattatctca tttcttatga
    35041 caagctaagc aaattatctc caccaaataa tttattcata tttttgactt gcgcatacta
    35101 actaattctt aaattttcat acaaaatttc tcaatttccc ttaacgagta atatttatgt
    35161 acggtgattc tcactagtat cccaatcact ttttaagtta tcccgatttc aattctactt
    35221 caaaaatagt tgcttttatg tatgctgtta gttgattaga tggtcatttg ttgagctagg
    35281 tcgtttattt taaacaagtt gtttctttag cttataccta tctcgtctac caaacgcaag
    35341 atggcaaaca caaatgcata cgcatctgta tttgccactt tgattcgtat aaagctaaca
    35401 actattaact cctctcagct aatagttgct ctctttttta ttcagattca tttttgctga
    35461 tcacagccta gtatttcaat ggttttgaac actaaaccaa attgttccat tacaacattc
    35521 actggctgat gaaatggatt agtagatttc gattattcaa agcgattttc tattggtgga
    35581 tcgtgttccc aactaataag acggattcca aacctatcgt acagatcttc taaaccactt
    35641 ataaattttg cacctatttc aggtgtttta ttgcgtggat attctattgt tacgccatta
    35701 aatggatacc aattaggatt aggctctctt tttttctgta catctcccat agaatagtta
    35761 gcactttctg ggacataata tattcttcga ccatcctcta attcatctaa taagatattt
    35821 cgtgatcgtc tatcacttgc taaatgtaga cgccacgtgc gataccatgc catgctacct
    35881 gcatctgcag aaaatccatc aaataatgca acaattgtat cttctatcac aatcctttcc
    35941 ccatctactt catattctaa tctaaaatca aactcccctc gggtaatttc tggtcttggt
    36001 ggcttaggac ccaatattat tctacttaaa accgcattcg ttcccagatg aaacacagct
    36061 ataacaacaa taatgattaa tattttgttt ctatttgata aatttctcat tattcgcaca
    36121 tcacctctac attctcacat attagttcgt aatactcctt taatatcaaa atatctactg
    36181 gtcgtaataa tattcgctat gaattcctac tactcgtata agaaaggcca aatcatcagc
    36241 ttttacttgc tgatgatttg gcttgtttct atttatcgta attgttttct aatcaccaaa
    36301 attgttaaca atcggttcag atatttccca gttgattaag gtaattccgt attgtgtcaa
    36361 aacttcttgc gcttctccga aattaagtac agttcttatg tatccacttt ctcctaatat
    36421 aaagtctacc tctcttttaa aaccttcctc gagatttcta agggctatat gtggttttaa
    36481 tgacattgat tgtacatttt cccccatata atattgcgca cttcctcgac taaaatagat
    36541 tcctagatc
//
LOCUS       DAZABD010000023        35723 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000023 DAZABD010000000
VERSION     DAZABD010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35723
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.023"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(7..2172)
                     /gene="copB"
                     /locus_tag="ACHALG_001211"
     CDS             complement(7..2172)
                     /gene="copB"
                     /locus_tag="ACHALG_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HFN2586635.1"
                     /translation="MNGNDMEKKQEHKKEAGNKESAHSNEKQMKHDHSQMDHSMHMEH
                     NHGDIADSKQMDHSMHMDHDHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGMEF
                     PFQITFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYIYS
                     VYSFIANLINPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPESV
                     KRLTKEGKEETVSLKEVKEGDHLIVRSGDKMPTDGVILKGETIVDESAVTGESKGIKK
                     RANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAKWL
                     FYVALTVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAAKN
                     GLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGALEA
                     NANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKSQQVKIVNEKEVERLGLN
                     VEQALLKPYQEQGNTISFLILENHLAAIVALGDVIKTEAKEFIRTLKERKITPVMLTG
                     DNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLARAS
                     IGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILAIP
                     LAAGILAPIGILLSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(2192..4378)
                     /locus_tag="ACHALG_001212"
     CDS             complement(2192..4378)
                     /locus_tag="ACHALG_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFN2586636.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQSYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQENALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRMTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(4378..4587)
                     /gene="copZ"
                     /locus_tag="ACHALG_001213"
     CDS             complement(4378..4587)
                     /gene="copZ"
                     /locus_tag="ACHALG_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HFN2586637.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(4600..5040)
                     /locus_tag="ACHALG_001214"
     CDS             complement(4600..5040)
                     /locus_tag="ACHALG_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HFN2586638.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(5114..5653)
                     /locus_tag="ACHALG_001215"
     CDS             complement(5114..5653)
                     /locus_tag="ACHALG_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFN2586639.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
     gene            complement(6268..7734)
                     /locus_tag="ACHALG_001216"
     CDS             complement(6268..7734)
                     /locus_tag="ACHALG_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFN2586640.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            complement(8045..10126)
                     /locus_tag="ACHALG_001217"
     CDS             complement(8045..10126)
                     /locus_tag="ACHALG_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFN2586641.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
     gene            complement(10268..10390)
                     /locus_tag="ACHALG_001218"
     CDS             complement(10268..10390)
                     /locus_tag="ACHALG_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2586642.1"
                     /translation="MNLDKLPATGFKLSCYPVKIKKASAGWIRAGAMIEEKKKE"
     gene            complement(10651..11010)
                     /locus_tag="ACHALG_001219"
     CDS             complement(10651..11010)
                     /locus_tag="ACHALG_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFN2586643.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(11040..11381)
                     /locus_tag="ACHALG_001220"
     CDS             complement(11040..11381)
                     /locus_tag="ACHALG_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2586644.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(11378..12052)
                     /locus_tag="ACHALG_001221"
     CDS             complement(11378..12052)
                     /locus_tag="ACHALG_001221"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFN2586645.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(13228..14526)
                     /gene="asnS"
                     /locus_tag="ACHALG_001222"
     CDS             complement(13228..14526)
                     /gene="asnS"
                     /locus_tag="ACHALG_001222"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFN2586646.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(14566..15756)
                     /locus_tag="ACHALG_001223"
     CDS             complement(14566..15756)
                     /locus_tag="ACHALG_001223"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFN2586647.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNSEIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(15777..16304)
                     /locus_tag="ACHALG_001224"
     CDS             complement(15777..16304)
                     /locus_tag="ACHALG_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HFN2586648.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(16351..19140)
                     /locus_tag="ACHALG_001225"
     CDS             complement(16351..19140)
                     /locus_tag="ACHALG_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HFN2586649.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTSMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     ETAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHTLPKKVPIKELAMEKI
                     YQDIHEFLKKNEEGS"
     gene            complement(19287..19487)
                     /locus_tag="ACHALG_001226"
     CDS             complement(19287..19487)
                     /locus_tag="ACHALG_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFN2586650.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(19938..20258)
                     /locus_tag="ACHALG_001227"
     CDS             complement(19938..20258)
                     /locus_tag="ACHALG_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2586651.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     PDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(20761..21879)
                     /locus_tag="ACHALG_001228"
     CDS             complement(20761..21879)
                     /locus_tag="ACHALG_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2586652.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            22112..22234
                     /locus_tag="ACHALG_001229"
     CDS             22112..22234
                     /locus_tag="ACHALG_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2586653.1"
                     /translation="MHLIMIFSLLSIISLFAFSASRLLEAETQPVPVKVRKNDR"
     gene            22352..23659
                     /locus_tag="ACHALG_001230"
     CDS             22352..23659
                     /locus_tag="ACHALG_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFN2586654.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSVT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKIAGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLSADGHAENPSARFNETARLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAE
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            23780..24979
                     /locus_tag="ACHALG_001231"
     CDS             23780..24979
                     /locus_tag="ACHALG_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFN2586655.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYSSTWTFAVI
                     LTYFVVLEIFYSTFYFLIPLVFFSIFAFSYFSEKRRLLNGLLFNVFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVFLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLIFDFFLLKYLPQWINALFFCIPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYWAQSRATLNPPILLMSGGQGADENVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMRYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(25004..26272)
                     /locus_tag="ACHALG_001232"
     CDS             complement(25004..26272)
                     /locus_tag="ACHALG_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HFN2586656.1"
                     /translation="MEKQLPSPVRILMYMLKWLVITAILGVFMGSLSAFFLNSLTFVT
                     DIRLAHPWLLYLLPISGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKAEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAYAYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRIKEKNKV"
     regulatory      complement(26327..26393)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(26435..27082)
                     /locus_tag="ACHALG_001233"
     CDS             complement(26435..27082)
                     /locus_tag="ACHALG_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HFN2586657.1"
                     /translation="MEQTMKPYQYFFIKREVDQLLNAYSSVNDPKTVQTVQALAAEKI
                     RDVLGYEPPEIDTFLTTVLDVKLTKTQAERALEELKQIVQPFMQPSKPQIDKLFRKVK
                     KLKQPAWNEIDLQAHTYIGWNDPGTQKKFMVYYEDEKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKKER"
     gene            complement(27489..27992)
                     /locus_tag="ACHALG_001234"
     CDS             complement(27489..27992)
                     /locus_tag="ACHALG_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFN2586658.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TKLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(28115..28879)
                     /locus_tag="ACHALG_001235"
     CDS             complement(28115..28879)
                     /locus_tag="ACHALG_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFN2586659.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMTLTSE
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLADVIPVMKGKGFTVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            28986..29261
                     /locus_tag="ACHALG_001236"
     CDS             28986..29261
                     /locus_tag="ACHALG_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFN2586660.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            29699..30649
                     /gene="yidC"
                     /locus_tag="ACHALG_001237"
     CDS             29699..30649
                     /gene="yidC"
                     /locus_tag="ACHALG_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFN2586661.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPADNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(30828..31802)
                     /gene="lacD"
                     /locus_tag="ACHALG_001238"
     CDS             complement(30828..31802)
                     /gene="lacD"
                     /locus_tag="ACHALG_001238"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFN2586662.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDTKMPGR
                     LPDLLNIWSVKRLKEAGADACKFLLYYDVDEPAEINVQKQIVIERIGSECEAEGIPFF
                     LEILSYDATIGDPSSLAYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QETCYSQAEASAYFYEQSKVTEQPFIFLSAGVNATLFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLKETATPVYVDR"
     gene            complement(31799..32257)
                     /locus_tag="ACHALG_001239"
     CDS             complement(31799..32257)
                     /locus_tag="ACHALG_001239"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFN2586663.1"
                     /translation="MTIIHEENIFLQQPLDSQEEAFKFLAKQTFCLGIAHDEKEVYQK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSMIIVTLNKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRKAKSKTEIATILTKEIGEDL"
     gene            complement(32254..33716)
                     /locus_tag="ACHALG_001240"
                     /pseudo
     CDS             complement(32254..33716)
                     /locus_tag="ACHALG_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(33698..34648)
                     /gene="pfkB"
                     /locus_tag="ACHALG_001241"
     CDS             complement(33698..34648)
                     /gene="pfkB"
                     /locus_tag="ACHALG_001241"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFN2586664.1"
                     /translation="MSIYTCTMNLAIDLFIETEEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQQKQIQTEFIEVPGMTRINVFTQVNQTGEE
                     YKLVNQGPEIPQTAVHNFLNQIRALQAEDYLCVSGSLPRGLSPKVLIEISRICQEKGI
                     HLIIDSSDQEVMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVELGAENV
                     LLSLGEKGAILFTKDRVFRGNSPKGKVVNTACAGDTMLGAFLAGYMNRRPLDETLRKS
                     IAAGSSTAFRKGLTDFLDVEELSKQIKIREERFERWENIN"
     gene            34791..35540
                     /locus_tag="ACHALG_001242"
     CDS             34791..35540
                     /locus_tag="ACHALG_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFN2586665.1"
                     /translation="MDHKLSETEQYLWNFIEHHILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINMLEIG
                     NIEDAIQRIKFADRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISRKLT
                     KKDSVIFVSLNGETKELVTAAKNCYDAEIGSILVTANHSSTLKDYCEINLVGFKSEGS
                     YFPNYEVRSRLPLQIIARILLDAYVLRMGEN"
BASE COUNT        10221 a         7682 c         6236 g        11584 t
ORIGIN      
        1 tcattattat ttgattttca atgtcaaagc attcagcgct acgactaccg tacttaacga
       61 catcaagaca gcaccaactg ctggacttaa taggatacca attggtgcaa gtattccggc
      121 tgctagtggg attgcgagaa tattgtaccc tgctccccac cagaggttct ggatcatttt
      181 ctttcgcgtt tgcttagcta aatcaaggaa atgcaaaata tcttttggat cgctgtttgt
      241 caagacgaca tccgcagaat cgatcgcgat atctgttcct gcacctattg ccatcccaat
      301 agaagcacgg gctaaacttg gcgcatcatt gatcccatca cccaccatga ttactttttt
      361 cccttggtca agatatttct ggacaacagc ttctttatca tctggtaata aaccgccgta
      421 atattcttcg attcctaaat aatctgctac tgcttgagca gcatttttat tgtcgccagt
      481 aagcatgact ggagtgattt tcctttcctt caatgttcgg ataaactctt ttgcttctgt
      541 ttttattacg tcacctaaag caacgattgc agctagatga ttttctaaaa tcaggaagct
      601 gatggtattt ccttgttctt ggtaaggttt caataatgct tgttctacgt ttaatccgag
      661 acgctcaact tctttttcat taactatttt cacttgttgg cttttgactg ttgcctctag
      721 cccaactcct gaaagatttt ttagattttc ggcttgataa ggcttgattt catgttcttt
      781 caaataattc ataatgccaa cagccaatgg atgattggca ttcgcttcca atgctcctaa
      841 atattgaagg atttcatttt tattgaacgc atcatcaaga acttcgacac ccgttaccgt
      901 aaattttcct tctgtcagtg tcccggtttt gtccaacatg atcacatcta aatcattggc
      961 ttgctccagt gcattgcggt ttttcaatag caatccgttt ttagcggcga tcgatgtgga
     1021 acgagcaaca actaatggga tagctaaacc tagagcatga ggacaggcga tgataaagac
     1081 agtcaccatg cgttccaatg cattaggaag atctgtcaag aacaaccatg cgataaatgc
     1141 caagataccg acagttaagg caacataaaa cagccatttt gccactttgt ctgataatga
     1201 ctctagctta gatttttctc cttgtgcttt tcgcaccatt tccataacct tcgctagata
     1261 cccattttca ccagttccag ttacttcgat ttcgatcgtt ccatccccat tgatagaacc
     1321 gccgatcact ttgtcattgg ctcgtttttt gattcctttt gattcgccag tcacagctga
     1381 ttcatcaacg attgtttcac ctttcaagat aacaccatct gttggcattt tgtcaccgga
     1441 acggacgatc aaatgatcgc cttcctttac ttcttttagt gagactgtct cttcttttcc
     1501 ttctttcgtc aagcgtttca ctgattctgg cagtaattct gctagttttt gtaaagcatt
     1561 gctggcattc gaaacagcgt tcatttcgat ccagtgacct agcaacataa tgacgatcaa
     1621 tgtagctaat tcccagaaga aatccatgac gtgcgtatga ggattgatca gattcgcgat
     1681 aaacgaatag acactataaa tatatgcaac tgtaatcccc atcgcgatca gcgtcatcat
     1741 agcaggactt ttttgtttca attccatttt tgccccgctt aaaaatggct gtccgccata
     1801 gataaacagt atagttgcta agatcagtac gagccaatcc gaaccaggaa atgttatctg
     1861 gaagggaaat tccattccca tcattggtga aaaaagaatg attgggatag ctaggataag
     1921 cgatagccaa aatttttgtt tgaaattccc catgtgcatg gaatgatcca taccgccatg
     1981 atcgtgatcc atgtgcatcg aatggtccat ttgtttgctg tccgctatat ctccatggtt
     2041 atgttccata tgcatcgaat ggtccatctg gctatgatca tgcttcatct gtttttcgtt
     2101 tgaatgagca ctttctttgt ttcctgcttc ttttttatgc tcctgttttt tctccatatc
     2161 gttcccgttc atttttactc catcccttct cttacttttt catgtgccga tttaagctca
     2221 gggagttcaa caatacactg acggaactaa acgccatcgc tcctcctgca atgattggat
     2281 tcaaaaagcc taatgcagca aaaggaatgc caatcgtatt atagacaaaa gcccagaaaa
     2341 gattttgttt gatttttctc aatgtcattc tagacaactc gattgtttga gcaatggacg
     2401 ccaaactact attcatcagc gttacatctg ctgtttccat cgcgatgtcc gttccacttc
     2461 ccattgccat accaacatca gctaatgcga gagctggcgc atcattgatt ccatcgcctg
     2521 ccataccgac ttttttgcca tctttctgca gtttttccac ataggctgct ttatcctctg
     2581 gaagtacttc agcaaagata tgctttggat caataccaac ttgtttacca atcgtttcgg
     2641 ctgccagttt gttgtctcct gtcagcataa aaacatctaa tcctttattc tggagctgct
     2701 tgattgcttg tttgacttct agtttgattt gatcagccac ggcaatcaac ccgatcactt
     2761 gcttttcatc agctaaaaac atgactgttt ttccctgctg ttctaattct aacgcgtttt
     2821 cttggtattc atcaaatgat aggttcaatt caattaatcg tttacgtgta cccgcaaaat
     2881 agcgtactcc gttaatcgta ccgctgattc cagcacctgg gtgggctgta aaatcaatca
     2941 tatcgtatgt tgctgcttct tgcagttttc cgtattccac gattgcttta cctagtggat
     3001 gttcagaagc ttgttctaaa gtataaaaaa tagataggac ttctttagaa ccgactagat
     3061 ctgtaacttt gggctttcct tctgtaatcg ttccagtttt atccaaaaca atgctgtcta
     3121 agtgagcagc tgcttctaat gcttctccgc ctttaattaa aattccattt cgtgcaccaa
     3181 cacccgttcc taccataata gctgttggag tcgctaaacc taatgcacat ggacacgcaa
     3241 tgactaatac agagacacta tggatcaatg cgagttgcca atcaccagtg atcaaacctg
     3301 tagctatgag cgtgataaac gcgatgacta atacgatcgg aacaaaaatc cctgagatcc
     3361 gatctgcgat tttttggatt ggggctttgc tcccttgagc atcttccacc atttgaatga
     3421 tttgcgctaa tactgtctgc ttgccgactt gtgtaacttc catgtgaagt agaccattcg
     3481 tattgattgt cccgccaaag agcgtgtcat ccggattctt ttctacagga agattttcac
     3541 cagtaagcat actttcatct atcgcactgc ttcctgaaat aattcgacca tctgctggaa
     3601 tctgctcacc aggacggatg actagttgat caccaacgac tacttcttca atcgctaacg
     3661 tttcttcttt cccgtttctt atcacttggg cggtttttgt ttgaagagac atcaattgtt
     3721 tgattgcatt acctgttttc gtttttgctg tatgttccaa atatttgccg agcaaaatca
     3781 gcgtgatgat catactgctg ctttcaaaat aaagatcttg ggggtgtcca cggaaaaatc
     3841 cgttgtaaat gctcaatgca aaggcagccg atgttccaat tgctaccaat acatccatat
     3901 ttggtgcttt cgtcttgatt gcatggtacg cacctttata aaaacgagca ccgacataaa
     3961 attggacagg agcagacagc actaactgga cgattggaaa atgaaaaaag tgaacaatcg
     4021 ccgcatggct tcctaacatc atagcaatca ttgacaacat caacggcaaa gtcaaaaccg
     4081 tactcaatat aagatcgaat agcatttttt tcaaatacga ttgcttttct tctgcaattt
     4141 tttgtttgtg ggcttcatcg tacaaaattg caccatagcc gatgttttct acgctttgga
     4201 ttaatttttc agtggtcgtc ccttcatatt tgacacttgc tttttcagtg gccaagttga
     4261 ctgtggcatt gaccacacca ggttgtccgt tcagctcttt ttcgatccta gcagaacaat
     4321 ttgcacaagt catacctgta atgacgaacg tatccatttt ggtttctgct gttgccatca
     4381 aatcacctct gctttataac ccaatccatt gattgcttga caaatctttt cggattgaac
     4441 ttttgtttca tcaaacttga ccgtaccgtt tgcttttttc aaatttactt tcactttttg
     4501 cacgccgtca agtgcgctta ctgtttcttc tactcttgct gcacaatgat tgcagctcat
     4561 accttcgatt gaaaattgtt gtttcatttt ttcttcctcc tattgatgtt gtttacattc
     4621 acattgacct gggatacagt tacattcgat cgtatctact ggttgctttt gttccagttg
     4681 tttcaaaatc tcattgatat cttcttgagt caaagtggct tcttctacta gatccgcaat
     4741 cgtctctcca atcctttttg cacagatatg ggaaaaaaga ttttccgtcg cactgcgaac
     4801 tgtttccatc tccgagacag ctggatgata aatgaatttt tttccttctt gttctgtcca
     4861 gatcacttct ttttttacca aacgacccag caaagttttg atcgttgctg gtttccaatc
     4921 catcgaatca gctaagatct gcgtgatttc ttgggctgtc gtctgtccaa gtgtccaaat
     4981 aacacgcatg atctcccact ctgaatcact gatctttact ggcgttttga ctttttccat
     5041 ttatttcaca cctttcgttt acaaatgtaa tcattatctt atgaatacag attacacttg
     5101 taaacaaaaa ctgtcaatta tttttgctct ctattttcaa tttagttaaa aattcttgtt
     5161 tctctcgtct tgttttaagg atgatcagtt tatttcttgc attagctact tggatcagct
     5221 gctgtatttt cggttcattg ttttcttcaa aatgccagat aaaagataaa aattctaagt
     5281 attccctgtt tagtttctcc gaaaatgatt caggcatatc tggccgagat tttttgttta
     5341 tctttctttt tagagaacgc caaactactc ggtagatggc gagataacgc ggcactttaa
     5401 gccaaatgat cttatctgct tctttaatcc tttcctctaa tgtagaagta taatttcctt
     5461 caacgatcca tgacggatta gataccataa acaattgctg ttcttgcaaa aaccactttt
     5521 ttgcctgaat actgtagtct gttgtatgcc ataaaaggtc tagcggcagt agcggaaagt
     5581 cggtgatttc tttgatcttc cttgcgaggg tcgattttcc cgacccactt tgtccaatca
     5641 cgatgatctt cattcttctc cctcacttct ctttggaatt tggttcgatc ataccactta
     5701 taacgccgca aaaaaacaag ggactgtgac aaaagtcgtg tcaaagtccc ttgtttcgaa
     5761 taaatggtgt tcaatcagct gaccttcagc attagctcaa aaagcggcaa aatatgagga
     5821 gctatttttc cgtttgtttc tctaatactc aggtcaaatc gctgatttta caacctcttc
     5881 cttaaacggt gttctcctgc caacacctcg gtcacaagtc acaatagtca acaaacatga
     5941 gaagttgttt tttgaaattg ctccttgtga ctcggagtta aacggcaggc tcactacctc
     6001 tttcttaaac ggtgttccaa agcttgcatc tgcggtaata agcccagcca aacgaaaaat
     6061 atgaggggct atttttgttt ggctgttctt attacttgat gcaggacagc tttttcacaa
     6121 cctctgattc acggtgttcc agaagtagct tcttcgaaaa acaagccgaa acttcacaaa
     6181 aatttgagaa gcaattttcg taaattcctt cttatttctt gaagctgacc acttctgtca
     6241 caacctcttg taacaagtat ttctatttta aggctgaacc actgtttctg gttcagtgat
     6301 ttctttgttt ttgtctcgac gagcaattgc tggcaagatc atacctaata agatcaagac
     6361 gattggtgtg atgatattcg taaataattg gaaccaccat gctttaggat cagcagtata
     6421 atctactttt ggcaccattc ctaggataca tgcaaacgca gtgaaggcaa agcaccaagc
     6481 accaaagata aaaccgattt ttggattttt aacaaattta tattcagaat tgaatttctt
     6541 ataagcttta ttcaacatca tatatgccaa gaacacccat aggtaacgca ttggcatgac
     6601 aactgagttc aagttcgtca accattttac taattcattc atatctttga taccaaaaat
     6661 cggcaacaaa ataatgattc ctactagtat acctgtcaga agataaccat tcaccaatgt
     6721 acctttacga ttacgtttac gtagccagtt tggtacaaat tctggatccg catctgctaa
     6781 caagattttt agtggtgcat caattgaaaa ggctaatgcg gagatttgtc caatcatgtt
     6841 tgtcaatgcg taaatgatca tcaatgagtt accgatacca tagtaattac ctaaaatttg
     6901 aaaagcactg tatgccccat ttgccatcaa gtctttagga atattatcac tagcaaacag
     6961 catacccatt gcgactgagc ctaatacagc acaaatccct accattgctg ctaagaagat
     7021 catcccttta ggaaattcct ttgcaggatt tcttgtagag ttgacataag gggatatttt
     7081 ttccgcccct ccaactgcaa atactagcat cgaaacggtt gtgaaataac tgaaatcaaa
     7141 tgttggaata tatgttttca cttgattcat atgtggtgtg gcaaattcca tcgttggatt
     7201 gatgaacggt gccgttacag ctaaaagaat aaataagatc gacatgacga acatcgctgt
     7261 tccggccagt ccgccaatca ctttcaaagt agaaagtcct tttgttgata aatataaaaa
     7321 ggcaagaaat ataataagtg aaataactgc taccatttgt actgacatcg tatttacgat
     7381 atttccattt ccttggactg cccaaccacc agcaatcaga attgcttgag gcttttgtgc
     7441 caaataagga atatggacga cccagtaagt ccaagcggca tagtatgcca gtcgtttatt
     7501 agatgtcgta ttttcgaccc atgaactgac gcctccttta ctatccttga aggctgatcc
     7561 taattgtcca acgatcaatg cgtatggaat aaaataaatg gccaaaatca atagccaaga
     7621 ggtgacaact gatactcctt gctgagcata gttattaact acattcccca tgccccaaac
     7681 catgttaaat gcaattaacg caacagtaaa ccagcgtaat tgtttgttgt tcattcgttc
     7741 tctcctttat gataaaaatt tgttatccag atgtaatcat attcaatttt taacaaactt
     7801 acaagacagt gttattttat gtgtgtattc ttttatttaa tacacatcgt tttttcatat
     7861 tttttccatc aaaaaaataa gttattttct tttatttcat tgaccttatt gaatttcaag
     7921 ctattttgaa ttttctgcaa aattaaaaac attagttatt ttataatcct atctattttc
     7981 tttacatttt tcattaagaa aaggatataa aaaaaagagg acgttcattc gtcctcttca
     8041 ttgatcaacg tttatttttt acatgcaagc ttcgcatggc attcaaaaca gctaatactg
     8101 tcaccccaac atctgcaaat actgcatctc ccattgatgc aaaaccaaaa gcacctaagg
     8161 caagtaccaa aatttttatc ccaatagcaa agacaatgtt ttgtttgacg attttcaatg
     8221 ttttctttgc taaatggatt gcttctgcaa tcttcgatgg ttgatcattc atgatgacta
     8281 catctgctgc ttcaattgca gcatcgcttc ccaatccacc cattgcgatt cctacatctg
     8341 ctcttgctag caccggtgca tcgttgattc catctccgac aaatgcggtc tttttattgt
     8401 ttctttgtaa gatttcctct aaccgctgca ctttatcttc aggaagcaat tcgctatata
     8461 tcttgtccac gcctacttgc ttcccgatat gatcagcaat ttttttcgag tcgcccgtca
     8521 acatgactgt gttcttcaca ccagcctgtt ttaattgaac caatgcgtct acggcatctt
     8581 ctttcaaggt atctgcaata acaagataac cactgtaact ttgatcgatc gctagataaa
     8641 gcaatgtacc gatttcttga cttaaaggaa aagagatttc aaatctttcc atcaatttgt
     8701 gattacctgc taatacttgt ttgccatcaa cttcagctaa gatcccttca cctgcaaatt
     8761 cctggatatt agttgttggt aaaagcggac ctgcgtagcc ttctaaaaca gattgagcaa
     8821 tcggatgtgt agaaaactgt tctgcactcg caatatattg catgaatttc tttggatcga
     8881 tttttgtatc gatcgtctgg atcgaaaagt cacctttcgt taatgtgccg gttttatcga
     8941 ataccactgt ctcggtttgc gcaagcaact ccaagtaatt tgaacctttt actaatacac
     9001 cgattttact tgcgcctccg ataccgccaa agaaactaag tggcacggaa atcactaatg
     9061 cacatggaca tgagatcact aaaaatgtca atgctcgata gatccattca gaaaacgccg
     9121 ctccagaaac aacaagtgga ggtaaaacag ctaataaaac ggccaatcct acaacgactg
     9181 gtgtgtaata gcgcgcgaat ttagtaatga aattttcagc tggtgctttt ttactgctgg
     9241 cattttctac taattctaat aatttgctga tagtagaatt ttcataagat ttttctactt
     9301 gaactaaaaa tggtttattt ttattgataa atccgcttaa tactgtttca ccaacatata
     9361 cagatttagg gactgattct cctgtaagtg ctgacgtatc gactaatgat tctccttcga
     9421 tgatcgttcc atctaacggt acacgttcac caggcttcac aagtacaaac tgaccgatct
     9481 gcacgtcttc agggttcacg gtcatcaatc cttccgtcgt ctgcacgtga gctgtttctg
     9541 gacggatcgc catcaattcg cgaatcgatt ttcttgaatg actgactgcc aatccttgga
     9601 aatactcgcc gatttgataa aatagcatca ctgctacggc ttctggatat tcaccgatca
     9661 acatagcgcc aatcgtagca acagacataa gaaaattctc atcaaagatt tttcctttga
     9721 cgatattcat cactgccttc ttcactacat catatcctat cagcagataa atcaataaat
     9781 accctatcaa gcgaattggt tcattaaaat caaataatag caataaagcg atacctgcaa
     9841 gggaaaataa aatccggaac agcgtatttc tctctttttc atttccatgt tcagatactt
     9901 tttccttatc tatttctgtt actttgacat ctggttctaa ttccttcact actttttttg
     9961 tttcttgttc aatcgtagcc aaatgatctt cctctgcgtc aatcgttagt tttccagaag
    10021 tgaagtttac cgttgcttcc ttgactccat tgatcttctg aactcctttt tcgattttca
    10081 ttgcacagtt cgcacaatct aaaccttcta atctgtaact tttcatttta atgacctcct
    10141 cgcctcaaca tatgaatatc tcttcataac taattataaa tgaatggtta ttcatatgtc
    10201 aaggtatccg aaagaaaaaa tgaaaaacat ctgcatcgga aaatttcttc catacagatg
    10261 ttttggctta ttctttcttt ttctcttcga tcattgcccc tgctcttatc caacccgcac
    10321 ttgccttctt gatcttgaca ggatagcaac taagtttgaa gcctgttgct ggcaatttgt
    10381 ctaaattcat taatttttcg ataggacaat atcttttttc aatccctgca aaatgtcctt
    10441 cccagatatt cgaagctcca ctacgctttc aaaaatatag aaagagttct ttaatgaaaa
    10501 ttatttcaac tttccataaa cagtaatccc atataaataa aagacgtaaa agtcacatgg
    10561 gagaacttat acaggaaaac acatagagtc aggtgaaata catgtatccc acctgactct
    10621 atgcgttttc taacctttat tattctcttt ttatacaatt tctgcaccga atggcatcaa
    10681 cgccaatcga gcaagtttga aagattgctt ggcaaaaggt atccctatga tcgtgataca
    10741 tagaaggatg ccgctgacga catgtgcgag agcaattggt aggcctgaaa cgatcagcca
    10801 gatgatatta acgatcaatg acatgccaga ctggtgataa attacttctt tgccaaaagg
    10861 cgcaaaagaa agggaggcga tcttaaaaca ttgcataccg attgggatac ctatgatcgt
    10921 gatacaccaa agtatccccg ctgctaacca gctaagacca ccgattagtc cgccgaatat
    10981 aaaccagatg acatttccta aacaactcat tgtatttctc cttctattac tgtttttgat
    11041 taaaactggg caaaaattga ttttattctt ctttttttag cttccagcat caagtattct
    11101 gcaaattttt tttggatttc ttccgtattt tcttttgtta ctaatgtgtg ttggattagt
    11161 tttcctgatg gtcgctgatc cacccgttct tctgactgtt gcacgtccgc ttgcgcaaat
    11221 ccgataatcc gtttttcttt tgttacagca acgagcaata gtttattctt atcatttagg
    11281 tcagctgccg tttttgcttt tacgaatgat tctggatgat cagtaatcag ctgattgatt
    11341 tgaggtaaat cttcttttac tgcccaccgt atttccatca tttctcactt ttctttcgct
    11401 catttttatc tccactaaaa aaatcgactg cggcaattgc atctatccag ctttgtgcaa
    11461 tgatctcatg tccgccaatc gttgggtgga ccccatcttc tcttgtatat ttcgaaaaac
    11521 cataagcaat ccctaaagca ttgaatatgc catctaaagg aaccagcaat gcgccatatt
    11581 ccttagcaag tttacgaaca atttggattt tagggtctaa gtctttacgc cagccatgac
    11641 ggtcgtcggt tataggaagt acaaacggct ccatcaagat gatgttgcag atttccgctg
    11701 tttgaagttc atttagtaat tttcgataaa cggattcaaa ttgctgcgca gatttctcag
    11761 ttccgaatat agcctgatcg ctagtattat gccaggtatc attgattccg atcaaaatcg
    11821 atacatagtc aggttgtaaa gctaaacaat cttcttgcca gcgtacttgc agatctgcaa
    11881 ttttgtctcc tcctatccca cggttcatgc attcaaattc catcgttgga tattgggaga
    11941 ttagttttcc ggcaatcaaa agtggataac ctttacctag atcagttgct tctgctcggt
    12001 ctcttcctgc gtctgtaatg ctgtcaccga taaataatat acgttttttc atttgtcaaa
    12061 aactcctttt tgaataaaac gatgattttc ttccttatat tatcccataa gccctatcaa
    12121 gacgaaagag aaaatatatg tataaaaaat gacaccagcc tagtcccttc aatcaattga
    12181 atgaacgctc atcaagtgtc tagtttttta tacttttttt cttaacgtgc cggattcccc
    12241 ttgattcctt cgttgtttgg tccagagaaa tcgatcacac ctaagcttcc gtcaatcaga
    12301 ccactgatga cgccatcaga acatatattt ataggctcat ctaagacttc cagtaaatgt
    12361 tcttttacta atatctcttc tagattgctt gggatagatt ggtcataact ttatctggtc
    12421 cgatgatttc aaggatgaat taaacttaca tatggatcat tgcatgctga aaatacggac
    12481 ctactccatc ttcgaatgtt actattagtt tgttttcagg tactagatgc tgctttattt
    12541 caacagcaga agcatcatca aatcagattt ccatttgaat aaccttcttt gtatatgttt
    12601 gattgattgc gatttcgtta tagaaccttg tgaatcaaat agcaatttct tttgctttct
    12661 tctcttgtct gctggaaact tcctttatcc cgtattttaa aaaaagaact gcgagagttc
    12721 gcagttcttt tttttaaata aacgttgctc tccggccaac tcctcggtca caagtcacaa
    12781 tagtcatcaa acatgaaaaa ctgttttttg aaattactcc ttgtgactcg gagctgaacg
    12841 gcaggcgcac aacctcttga tataaacggt gttcaatcag ctgaccctcg atattagcta
    12901 aaaaaacggt aaaatatgag aagctatttt tccgttttgt ttctctaata ctcaggtcaa
    12961 ggcactgatt tcacaacctc ttccttaaac ggtgttcaaa agccagtttc tcgatattaa
    13021 gccaaattta tgcaaaaatg tggaaagcca tttccacaaa atttttctta atactcgaaa
    13081 ctaaacagct ttttcacaac ctctccacta aacggtattc aaaagcttac atctgcggta
    13141 ataagtcccg ccaattcaaa aacatgaaca gctgtttttg cttggctgtt ctaattactt
    13201 gatgcagaac agctttttca tcacctctta tggataaatt cggttcaaca aacgtgggaa
    13261 tggaatagct tcacgtacat ggtcgatacc agcaatccaa gtgactgttc gttcaaggcc
    13321 tagaccaaaa ccagagtgcg gaacagaacc gtatttacgt agatcaagat accatgaata
    13381 ttctttttca tccaaaccgt gattacggat ttgttctagc aagaaatcat agtccgttgc
    13441 ccgttcgctt ccgccaataa tttcaccgta accttctggt gcgatcatat ctgcacaaat
    13501 taccacgtct tctcttgttg gatgtggttt catgtagaat ggcttgatag cttttggata
    13561 gtttaggata aagacaggct gttcgaatga attcgcaatg aacgtttcat gaggtgaacc
    13621 aaagtcttcg ccccattcga tgtcatcaaa tccatttttc ttcagtaatt cgattgcatc
    13681 atcgtaagta atacgaggga atggcaattt tgtataagtt tttaagactt ctttgtctcg
    13741 tcctagtaca tctaatgcat attcgcaatg atctaaaaca ttttgtacta agaatgctac
    13801 atattgttcc tgtacttcta aactttcttc ttgatgagtg aaagccattt ctggttcgat
    13861 catccagaat tcgatcaaat gacgtcgtgt ttttgatttt tcagcacgga aagtcggacc
    13921 gaatgaaaag acttttccga aagccatagc agcagcttcc atgtataatt ggccactttg
    13981 agataaataa gctttttgat caaagtaatt tgtttcgaat agatccgtag tgccttctgg
    14041 tgcagaacca gtcaagatcg gcggatcaat tttgacaaaa tgatgttgat taaagaattc
    14101 atacgttgca cgaatgattt catttctgat ctgcataatc gcatgttgtc ttgaagaacg
    14161 cagccataaa tgacgatgat ccatcaagaa gtctgttccg tgttcttttg gtgtgatcgg
    14221 ataatcgtgg ctctcgctta ccacttccac atttgtaatt ccgatttcat aaccaaattt
    14281 tgaacgactg tcttcacgga tttctccagt tactaacacg gatgtttctt ggtgtagatt
    14341 tttagctgtt tggaaaactt cttccgacac ttcgcttttt acaactacac cttggaaaaa
    14401 ggctgttccg tctcttaatt gtaaaaatgc gatttttccg cttgatcgct tatttgcgac
    14461 ccaagcgccg attgttactg tttctcccac atggtttttc gaatcgatga tttgaatttg
    14521 ttccacgtat gactctcctt ttatattcct tgaatttctt caggattaat tttgactttt
    14581 actctcaata aattgcttga ttctgcgtac cgcttcttct aacgtagcgc gatcagtagc
    14641 ataactcatg cggacatttt ctggtgcccc aaagccttcg ccagtcacaa gcgctacccc
    14701 tgtttcttct aaaagatctt ctacccattg tgtgacattt tcatatttac acatgcgtag
    14761 cgtttctttt acattaggaa aaagataaaa agctccttgc ggcttttcaa gagaaacacc
    14821 tggcagttct gccacaagcg gatataatgt attcaggcgt tcttcaaagg cgttccgcat
    14881 ttcttctact gtatcttgct cacctttcaa tgcttcaaca gctgcatatt gactgactgc
    14941 tgtcggatta ctggttgact gggaagcaac agcaatcatt ccattgatga tctcagaatt
    15001 ccccaccgca taaccaatgc gccagcctgt catcgcatat gttttagaca cgccattgat
    15061 gatgatggtc tgatttttga ttgcttctga aatcgttgca attggagtaa actcattgcc
    15121 attataaact aaacgaccat aaatatcatc agcaactatc aaaatatcat tttttactgc
    15181 ccattcaccg attgcctgaa gttcattctt gctataaatc attccagtcg gattcgatgg
    15241 agaattgatg attacggctt ttgttttttc tgaacgtgct tgttctagct gttcaacagt
    15301 aactttccag ctattggatt cttccccttt cacaaagaca ggaagtcctt ctgccagttt
    15361 tacttgctca ccgtaactga cccagtaagg cacaggaatg atgacttcat cttgcgggtc
    15421 aaggatcgtt tggaaaaggg tatacaaagc aaatttcgct ccgtctgtta caactgtttg
    15481 tgaaggttca tagacaagtc catagttttt cttcagataa tcaacgattg cctgtcgaag
    15541 ctctgggatt cctgctgttg gtgtataata gctagctttc ccattgcgga ttgcttcgat
    15601 tgctgcttct tggatgtttt ctggtgtcgc aaaatccggt tctcccacag tcaaactcag
    15661 cacatctctg ccttttgctt ttaatgcctt ggcctttgcc gcagctgcca aagtgacaga
    15721 cggttctaat cgttttgttc tttttgataa ctccatccca ttcattcctt taccttttaa
    15781 attcccttga ttgtcttgac ttcatctcca gattcaaagc caatcaggta ataattcagt
    15841 ttcccatttg tttcttttcc tactacttcc cacacaggct gatcatcata catccctaaa
    15901 gcagcttttt cgatttggat ttcaggatgg gccgcagcaa ttgtctgttt tgcttcgttt
    15961 tcagtcagtc cctttgtttg atccaatact tttacttttt caccagattt aggaatgata
    16021 acggcgattt cttttccttt atcatttgta cctgtcagtg aaaagtatgt cttctttcga
    16081 gtaaaccaat aaaaatgatc ggtttcctca agatttgcat atttctttgc gatttctact
    16141 gcttctttct tcgcctgcac cgccggacga ctggaacgca agtaaaaaat cgagctgatc
    16201 acgatgaccg ctaataaaaa tacgatcaag ccaagtaaca cgcgattaat cgttttttcc
    16261 tttgtctgtt ttttcttcaa gggtcgtcgc tcctttatct tcattccttt tcattatagc
    16321 agattttaat tatgaaaaaa gacagctcct ctaacttcct tcttcatttt tctttaagaa
    16381 ttcatggata tcctgataga ttttctccat agctagctct ttgatcggta cttttttcgg
    16441 taatgtatgg atgatccgtg cgccatattt tgcagtgact agtcttcgat ccaaaataag
    16501 caatacgcca cgatctgtct ccgtccttat caagcgacct aatgcttggc gtaacttcaa
    16561 agctgcttta ggtacggatt cctggtaaaa aggattgatc ccctctgatt ctaaataagc
    16621 gttcctagct ttgaccatcg ggcgaagcgg attatcgaag ggtaaacgtg tgactacaac
    16681 gatctgcaaa gcatctccag gcaaatctac accttcccaa aagctgtcag cgccaaacaa
    16741 tacgcttttt tctgacaaaa tgaagcgttt caaaagcttc tcccggcttc caccaaaccc
    16801 ttgagctagt atttctcgtc ctttttctag catagacaga tgcattttct gatagacctt
    16861 ctgcaaaaca tcatgagaag tgaataacac aagtattggc cgatcttctt gttcgatcag
    16921 acctgtaagt gctgtctcta aataatcgat gtatgcttga ttgctgactt gatggacggc
    16981 aggtgcctct tttggcacat agatccttgc ctgtttctca taatcataag gagaggcaat
    17041 cactttaaga ggtgctgctt caatgccaag ccgcttagag agatagtgtc gattagggcc
    17101 gatcttcagc gttcctccaa tataaagaat acgatcatat cgaggatacc aaacagtcga
    17161 cggcaacata gccgcttgaa aatcatggag atgaaaaatc atctgctgcg ctgtgttccc
    17221 aggaacgaac caatggatca aatgatctcg ccagtcttcc agccattgat ccatcagcaa
    17281 gtactgcttg tctaattgat caaataagct atataaactt gctaaatcga tccgcatctg
    17341 gtttgtccag ctttcttgga taatctcaaa agtcagacgc atttgtgact gtagctgctg
    17401 gatctcctga taaaaaagct gcaggtgatc aatggctgtc tcagattcca gtggcagctg
    17461 atccaacatc tcttttgtca tcatgacttc ttctggatac tgtccgttag gttgcttgcc
    17521 gctcactaaa ctcattaatc cttctgccat acaaatctgt gcttcgcata gcgcagaaag
    17581 ttcttctcga taaatcgata accatcgtaa ggcttcatgt tctttcggta aatagctttg
    17641 tattcggtca aacagctgat ttggttcttc taattgctgg atttgtttat ggaaatagtt
    17701 aatacttaat cgtttgtcat ttactttttc agcgatttcc ggcaaatgat gtacttcatc
    17761 gatcaacaaa aatggactag ctggaagcaa tggttctttt cgctgtgttt cttgtattaa
    17821 aaacgcatga ttgacaatca aaaaattact ttgcctgatt ttcttttgca agaaacgaac
    17881 aaagtcttgt tcgtaaaatg actgatttct tgctaaaaat ccaatacctc tgtgcgcgat
    17941 atctgcaaag aatgaatggt tcaaatttgt taaatgtaat tcatccaaat caccggtctt
    18001 cgtttgcgtc agccaaacta aaaccgccat ttgatacaaa gcatattgtt tttgttctgg
    18061 cggttccaca agtgtgcctt taaaacgctg taaatcaata tagtggcgat agcttttgat
    18121 aattacaggt tgtatgggct gatctagcaa ttgattcagc aatggaatgt ctttttctat
    18181 gatctgctgt tgcaaaacta gcgaaacggt actgatgaca gcgggttttt caggtgtagc
    18241 caaataactc aatggcagca gataaccgat tgttttaccc attcctgtag ctgcttcgat
    18301 catcaaatct tttgattcat cttttgtgaa atggtcgtag accgcattca tcagtctgtt
    18361 ctgttctttt cgatacatca gtgttttacc aaacattttt tcttttgctt gtttttttct
    18421 tggatatgtc cgctcgccat aatggacgga tgtgaatagt tccacttctt ttttctgcaa
    18481 agctagccca tcgacgattt ctaatgaggg atcaagtggc tctggttttt ctttcatttc
    18541 ttcaatcaca tgataaatga aacgactcgt atccatactt gtgtgtctac ttaatcgcgc
    18601 aatctgctct aacgtgatga ttggcaattt tctcatccgt tcttcgatca gcaaaagaag
    18661 ttgacctgtc acttcggcat cactatctgc ctgatgagga ttctcatgaa taaatccaag
    18721 gctttctgac aaatcagcta agcgaaagct tggttccgtc ggcaagaaaa tttgtgcaag
    18781 ttctactgta tcgatgcctg gtatcttcaa actaggaagt ccgcatcgca tgagttcatg
    18841 gttcaaaaaa ttataatcaa aataaatatt atgcgcgaca aatatggtgt ctgccaaaag
    18901 attgtaaatc gtttgggcga catcttcgaa ataaggcgct ttttggacgc gttgattcgt
    18961 gatccctgtt aaatgctgga tttgttttga aatcttccgc cctggatgaa tatcgatcga
    19021 aaaacgtgaa gtgatccgac ctgattcaat catgacgcag ccaaattgga tgatccgatc
    19081 ttcttttgga ttcgtcccag tcgtttcgat atcaacgact gcataagttc ttggtttcaa
    19141 aatatcttcc tcttctcctg cctctaatgt tcttttgaaa ttatactaca aagtgcagaa
    19201 aaatactctc atcgcttttt aaagaaaata gacaaaaacg cccagacacg caaatgtctg
    19261 ggcgtttatt ttttagtata ggttaattaa gctttagtaa cgtttgttgc ttgaggtccg
    19321 cgttggcctt cttcaacatc gaaagttact gcttggcctt cttctaaagt tttgaatccg
    19381 tcgccttgga tagctgagaa atgaacgaat acatcgtttc cgttttctgc agtgatgaat
    19441 ccaaatcctt tttctgagtt aaaccatttt actgtacctg tttccataat aaaaatcctc
    19501 cttgtgtatc aacacatatt ttgcaattac ttgaatggtg tatatctgga agatgtttat
    19561 ttgaaacaag ctaccacaga ttaccgaaca aaaactactc ctatagtata tcataccgtt
    19621 tacaaaaaag ctagattttt tcgacttttt ttattaaaaa aagttttctc agcgattaca
    19681 taaagtgaaa aaccgaactt tttttaaaat aaacgacata ttgttcagtt ggtttttttt
    19741 gctctgatgt tcttttttaa actaaaaaaa ttattctttc tgactactta tcggttctgt
    19801 ttcaacggtc gaggctgggg aagattcttt agtaggtctg atgttctcat ggtatttctg
    19861 atagcctatg acagttacca gaagaacaag gcaaaccaat acgatcaacg agattagttt
    19921 ttcttttttg ctcatatcta tacctcataa ataacaggat gatgtccatc atatttctga
    19981 gtatgagtaa aagcaaaagc tgcagctgta gaatgtaaga aatgccagca actttcataa
    20041 tcacaggtcg gacagtacca aaagtaacca aatcttggct gcaaagaccg ttcttctgtt
    20101 tcaggaatcg cagaatcaag atctggtgtc tgtacataaa catttttgta cagacgtgtg
    20161 cctgtaatag gagaccgctg tgtttcttca gcaaaaactg gctgacttgt taaacttagg
    20221 gatagaaaca gcaaacacca taacagtctt tttttcataa agacctcctt atcgataagt
    20281 ttttcccaat taaaatcata tctcaagaac gcccgttatt cacattttgt tcataaatga
    20341 tgttttttgt atcataatcg tcaaaaagag gtcgtgacca cgtcacaacc tcttttttaa
    20401 acggtgttcc agaagtagct tcttcggaaa taagccgaaa tttcacaaaa atttgagaaa
    20461 taatttttgt caatctcttc ttatttcttg aagctgacca cttctgtcac aacctcttcc
    20521 ttaaacggta ttcaaaagct tgcatctgcg gtaataagcc caccaaacaa aaaatatgaa
    20581 gagctatttt tgcttgctgt tcttattact tgatgcagaa cagctttttc acaacctctc
    20641 atacacggtg tttaatcagc tgaccttcgg tattagctca aaaaacggaa aaatatgtga
    20701 aactattttt ccgtttgttt ctctaatact caggtcaaaa cactgatttc acaacctctt
    20761 ttaataacga agaatcgctg tcagactctt ttcatctggc gcatcttttt gatccaaaat
    20821 aaacactttc ccgccattaa tgatcacatc atctgctaat gcattcaaca cttgacgccg
    20881 atcatattct gtttcaggat cttcaccgaa accatcagct aagtttgacg tagcgataaa
    20941 tagatgcgac acttttcctt ctttcgcagc tggaacgata tcaactaact gatcgatgaa
    21001 tttcttatct aaaagtttat tgtattcttt tgattcttga gctgcgagtt cacttgacag
    21061 cttcttcgcc ccttcttcga tatctctaaa ggatagctgc gcaggagaag cagaaatcgc
    21121 aacgtctgca caatagtaag gatttttagc tatttttttg aacatcgtct gattttctgg
    21181 taatgcaaat aaataaatcg gccattgttc tggattttcg aaagaatcct tcaagtacgt
    21241 atcaactgct tggtaatagt tgacccaatc gatctctacc tcttcatctt ttggatttac
    21301 gccatggaaa gttactccat ctcgggctcc gcctcctcca ttagaagaga agttcaaacc
    21361 gccacctgtc agttcagatc ccaatgcagt aacgacatca actggtgccc cttctggtag
    21421 agatacttct tttacttgtt tgttgctgac ctcatacaat ttcatggagt cgcggttcaa
    21481 tcccaataaa taataagaat agttgaactg cccatttttg ataatagcca gtaaataagg
    21541 aagatcactt acatagtatt gatcgtctac cgggatactc aaacgatgga tataggtttg
    21601 gtcagcagtt ataatgaccg ctacactcgt agttgcacta cgccagaacg aaggatctcc
    21661 taacaaattt tcaatttttt cttggaacgg ggaccagtca tgctcggaat attttttttc
    21721 aaaacgtttc tttgcttctt ttgcaaaatt tttgaatttg atttgatctt tttccacatc
    21781 ttgatgggcg acatgcgtat tcagcatgat cgtgacgaac ggtcctttag cgccttctga
    21841 agtcaattgt gacaatagtt ctttatcctt ttttgccata aacgtacccc tcctaaaaaa
    21901 atcattcctt tgtgtctatc taaagtctac cacaacacta tttttccata caataaaacg
    21961 tatctaaaaa aataaaacgg tttctttagt tgggtatttt gcaatttttt taagttatct
    22021 tcataatttc ctcaattatt taggcacagt cttcaactat tcttcatgaa aggctgttag
    22081 actaagaaaa agaataaaag aggtggacct tatgcatttg attatgattt ttagtttatt
    22141 aagtatcatc agcttatttg ctttcagcgc ttcccgctta ttagaggcgg aaacacagcc
    22201 tgttcctgta aaagtacgga aaaatgaccg ctaacctttt cataacgcgc gttcttatcc
    22261 taaagttttt ttcagaaaca ttgaaactct cttccctctc tgataaaatt ttaatttgta
    22321 gcgaaacaaa ttaaatacaa ggagatcgga tatgtccata aaaaaaacac gtctagtcct
    22381 attattggct agtctcattc tttgccttgc ttcttttcta ccagctgttg ctgtgcaagc
    22441 tgaagatact ttcaaagtaa atgcaaaagc agcctttgct gtagatgcgc aaagcggtaa
    22501 gattctttat gatcaagatg gagaaaaaac catggggatc gcttcaatca caaaaatcat
    22561 cggtctttac atcgtccttg atcaagtaaa agaagggaaa ctatcatggg atgataaagt
    22621 ttcgatttca gattatgcag aaaatttaag tgtcacccca gatttatcaa atgttccttt
    22681 gcataaagaa aatacctaca ctgttaaaga attattcgac tctgcaatca tccagtcagc
    22741 caacgcttca atggttgcct tggcagaaaa aattgctggc agcgaagcaa aattcactga
    22801 acgaatgaaa gaacaattga aagattgggg aatcaaagat gcaacgatcg ttaatgcatc
    22861 aggactgaat aactcttatc ttggcgaaaa ccgaccagaa ggtactggag aaaacgacga
    22921 aaatcaaatg tctgctcaag atgtggctat cgttgcccgt catttgatct tggattttcc
    22981 agaaatactg gatgtatcta gcactacgac acaaatgttt ggcgaaaaca cgcaatcacc
    23041 agtggaaatg gtcaactgga actggatgct cccaggattc gtgaattata aagaaggcgt
    23101 ggacggatta aaaactggta cgactgagct tgcaggtgcc tgtttcgtcg gtacgatcac
    23161 taaaaacgat caacggatca tcactgtcgt attgagtgca gacggccatg cggaaaaccc
    23221 aagtgcccgt ttcaatgaaa cagcccgctt aatggattat tgctacgaca attggtcaga
    23281 aaaagagctt ggaaaagcca atgcaagtat ccctagtcta aaaaccattg atgtaaaaga
    23341 cggaaaagaa tcaacagtgc ccgttgtgct taaaagtcct gtaaaagtat gggtacgaaa
    23401 cgatatggat actggaaaat taacaatcac tccaactctt gacaaaaagc aagtcactga
    23461 caatgagatc caagcaccta tggataaagg gacaaaaatc ggtaccgcaa cgatcacctt
    23521 ggcagaggat aaattaggtt atctagaaga caacaacgcg ccatccacag aaatcatcac
    23581 ggataaaacc gtagaaaaag caaatatttt cgttattggc tggcgacgtg tcgcagaatt
    23641 tttcggcaac ttgttttaat tgaacaaaaa gaaaaaagca tctttccgta tggaagatgt
    23701 ttttttgctt tttctagttt aaataacagc ttttttcatc tattttcgtt atactgtata
    23761 agaaaaagga gttgaattta tgaatctaac cagagatctg atttttttgg ttctctatct
    23821 tattggggta ttctccatcg tttattggcg aaaacagaaa aaggatcctt ccttgttctt
    23881 ttacagcagt acgtggacat ttgcggtaat cttgacttat tttgtcgtgt tggaaatttt
    23941 ttattcgacc ttttattttt tgattccctt agtgtttttc agtatctttg ccttttccta
    24001 tttttcagaa aagcgaaggc tgctgaatgg tcttttattc aatgttttcc tcattagttt
    24061 cggtatctac ttgtttgtct tgctatacga aacacaaaat atttttcttg gcggactgat
    24121 tgccttgatc acgatcccgt tactgcttgt ctttctattc ggcatttatg gattgatcgt
    24181 ttttcttttt tggaacggtg tcacagtttt acgtagagag tcacattcat tagctaatct
    24241 tttaacgttg atactagcca tttttcttac attgtttctt atttttgact ttttcttgtt
    24301 aaaatatttg ccgcaatgga taaatgcttt gtttttctgc attccattga tcttgatcta
    24361 tctatttatc gtattctaca actttttgac tgtctcattc ttgtatcagt tcaaccgccc
    24421 tcgatacaat caagacttca tcgttgtgtt aggcgctggg ttgatcaatg gagaaacagt
    24481 ttctcctctt cttgccaagc gaatcaataa ggcgattgct ttttattggg cacaaagccg
    24541 tgcaacatta aatccgccta tcttattaat gtcgggtgga cagggagcag acgaaaatgt
    24601 accagaagca attgcgatga aacaatatgc catggaacaa ggaattcctg aaagagatat
    24661 tcttgtcgaa accaattcga caaccacttt ggagaatatg cgctattcaa aggaaatcat
    24721 ggatcagcaa atgaaaggtc cttatcgcgc gatcttcagt tctaacaatt accacatttt
    24781 cagagctggt ctctatgcac gacaagcgaa actgaaggca aatggtattg gggcaaaaac
    24841 agctttctat tacttgccta acgcgttttt aagagaatac atcgccatct tgatgatgaa
    24901 caaaaaaaga catatgatca tttgcggatt gcttttcgca ggttccgcat tcttatcact
    24961 gatcacttta cttttctaaa aaacaaggtg tgacaaatat atgtcacacc ttgttttttt
    25021 cttttattct cttgacgatt ggcaagaata gtattccttt tttgacggct acttgctgtg
    25081 cagaataaat accgctgttt cctgaacaca taaagctgat caagcagatc ataaagaagt
    25141 aaacagcagc ttgtgaacca aataattcga ttcccatcac aaaacaggca attggtgtat
    25201 ttgtcgcacc agaaaatacc ccgataaaac ctaatccagc aagaaaagga acagaaacat
    25261 ggagcaaagg cgctagtgct gcgccaagtg ttgcaccaat ctcaaacagt ggtgtcactt
    25321 ctcccccttg gtaccccgca cctaaagaca agactgtgaa gaataatttc ccaataaaat
    25381 cataagcata tgcctgtcca ttaaatgatt cctgcagtaa cggaagactc aacccaaggt
    25441 aacgttgtgt ttgcaaaatc aacgctgcgg ctacaactac tgctccacct atcgtatttc
    25501 gccaaacagg atgaggaatc cattgcgcat accgcgcttt taaaaagacg atcgaacggc
    25561 tgaataccca accaatcaaa ccaaaagcaa tcccagcaac aaaaactttg aagaacagca
    25621 acatactcca ttcaggaatc acacccattt ggtaatgtag atgagaaaca ccatagcttt
    25681 ctgtcatgaa atttgcaaac agtgcagcaa agaagctagg gaagatcgct tctgttcgga
    25741 ctttcccgat tgccaacact tctaatccaa ataatgttcc agctaaaggt gtgccaaata
    25801 cagaactaaa acctgcactg attccactga taatcaagat ttcccgttct gctttatcca
    25861 atcggaacaa acgtgctgta ttatctgcta acgctccccc catttggact gctgttcctt
    25921 cacggccaac agatccccca aataaatggg tagtgatggt tccaaacaag gtcaatggaa
    25981 tgagccgcaa agggatcttt tcttctcctc cattcccttg atcgatcact aagttattcc
    26041 cacgactaga aagaccgcca tgatacgcat aaagataagc aaaaagcgcg ccgctgattg
    26101 gcaatagata aagtagccaa ggatgtgcta gtcggatatc tgtcacgaac gtcaggctat
    26161 ttaagaaaaa agcagacaag ctccccatga atactcccag aatcgctgtg atcaccaacc
    26221 acttcagcat gtacatcaaa atacgcactg gactcggtaa ttgtttttcc ataaaaaatt
    26281 cctcctcaat aaaaaaacgc ctactaaaga aaggggctgt ccccagactt tagtaggcgt
    26341 cattagctat ctttcgttag catttaaagg cgaacaccat cacctattca cttccattaa
    26401 aagatagcat atttttctgt aaaaaacaat aaaattatcg ttctttcttt acacgttcag
    26461 caaattcata gaaaggagcc aatttcgtca actgttgatt acattggtca ctatcatgac
    26521 aaatataatt cccctttttc gtatacgtgc catcagaacc tgctttcgtt gtagaaagaa
    26581 aaagcgaaac attcgatgtt ttctgacaga ttgcacagat tcccttattt atcgttggtg
    26641 ataaagtacc gctgatccct tttaattttt catcttcgta atagaccatg aatttttttt
    26701 gcgtccctgg atcattccaa ccgatgtacg tatgtgcttg caagtcgatt tcattccaag
    26761 ctggttgttt gagttttttc acttttcgga ataatttatc gatttgtggt ttgctcggct
    26821 gcataaacgg ctggacgatt tgcttcagct cctccaatgc tcgttctgct tgcgtttttg
    26881 tcagttttac atctaaaact gtcgttaaaa acgtatcgat ttctggtggt tcgtaaccta
    26941 atacatcgcg aatcttttca gctgctagag cctgaaccgt ttgtaccgtt tttgggtcat
    27001 tgacagatga gtacgcattt aataactgat caacttctct ttttataaaa aaatattgat
    27061 atggtttcat tgtttgttcc atgattttaa agtcctttct tctaaccaaa gtgcatactt
    27121 tgtagaaaaa agtgatcttc tgcaaagtat gtgttacctg aagatcccgg actcttttaa
    27181 ataagatacg ttttgcatct tatcatcacc ccctttaatg tttattcttc tatgtacatg
    27241 attcattcga taaataaaag taaaccgtat cttgaccaga tagtcaactg caaacagaaa
    27301 aaggactgtg acaaaagtcg tgtcaaagtc cctttctaca aacaaacggt gttccagaag
    27361 tagcttcttc ggaaataagc cggaatttca caaaaatttg agaagcaatt ttcgtaaatt
    27421 ccttcttatt tcttgaagct gaccacttct gtcacaacct caatatggtc tattattggt
    27481 tttacctgtt aaattgaaga ttcttttcca aagtaacgtt gccatctctt acgcatactt
    27541 gctgacatag gctgagcggc ttctttgact gcgcagtatt tgtctacgaa tgttacgata
    27601 tatccttctt tgtacttagg cggtgcaatc gttgcgcccc acatgtgctt caaaatgata
    27661 tctcgctcaa gatccgacaa tttggtgatc ttttctgcat tctttacagc gattcttgga
    27721 tggatatacg catgtgtacc ttcatcgaat ttcgtcgtac gccaatcata gtagaacaga
    27781 tcatgcaaaa gtccagcacg cgctgttgct ttagcattgc caccccattt ttttgcgagc
    27841 agataactat agtaagaaac actgatggaa tgctccaaac gagtggaatg aacatgctgt
    27901 gtgtaattcg ctaatttttt cactgcttct gtttctaaaa gatcttgtac ataagacata
    27961 tattcttcgt cttcacgcca tttttctgtc ataattatcg atctgccacc tttttgaatt
    28021 tcattttctt ctgaaattga tctaagtata ccaccagtgt tcggattatt ctagtcgctc
    28081 tagataaaat agctaaagct taatagttcc ttaatcataa agaaaaatga taaagaacga
    28141 tccctgctgc gactccgaca ttcaatgatt ccgcatttcc tttcattggg atatatacgt
    28201 tctgatcagt caaagacaag atttcctgac ttaccccctg accttcgttt cccatgatta
    28261 tagcgaaatc ctctgttttt tctactttgt ctaatgcatg tgcttcttca tttaattctg
    28321 taccgtacac cgtaaaccct ttgcctttca ttacagggat tacatctgcc aaatcttttt
    28381 gtaacacagg caaatgataa ttgcttccct gcatgcttcg cagcaccttt gtactataaa
    28441 tatctgccgt tcctttacct aataaaacac cagccatacc aaacgcatct gccgtacgga
    28501 tcatcgttcc cacatttcca ggatcctgta cattatctag taaaagccag cctcctgaaa
    28561 aatctggcgt atcttcagat gttaacgtca tcaccgctaa gatcccttgc ggtgtaggta
    28621 gatctgacat agcggtcatg acctcatctg aaactagaaa agtagaaatc tctttgttta
    28681 cagccagcca gtctccccat tctttcatac ctctctgtgt cacaaaaagt tcttttattt
    28741 ctgcatcaga cgacgcagct tcttcgatca gatggaaccc ttccaaaaga tattctgcct
    28801 tttcttctct gtatttcttt ttatgtagtt tttttaattc tttgattctt ggattttttg
    28861 ctgattggat ctctttcaca ttcctcacct caactgatta tatcatggaa taggtgcttg
    28921 agcttgtttc atcctaaaaa aatcggtatc attgatttat aaaacaggaa agaaaggtga
    28981 cgaacatgcg caaagttaaa atgaatgtcc aaggacgtgt ccagggcgtg ggatttcgct
    29041 atatgaccaa gatggttgct gatcagttag gcgtgaccgg aacggtaaaa aacgaagacg
    29101 acggctcagt cacaatcgaa gcaatcggaa atgacgacat catccaaaaa tttatcgaag
    29161 aagtcaagaa gtctccgagc ccaagtggac gagtgcagta tgtggatgtc caagaagatc
    29221 cgatgattga ggaacgaaag aaatttgatg tggtgggata aggttgtgaa aaggtcgctt
    29281 agttttgagt attaaggaaa attttgtgaa aatggctttc cacatttttg cataaatttg
    29341 acttaatacc gagaaactga cttttgaaca ccgtcgatca ggttgttttt gaggcgcttt
    29401 gcctcaagta ttaaagatag gtagtaaaaa agctgatcta taacttgcaa taaatttgct
    29461 taccccatat tttccagaca gaacaaaaaa gtttttagtt ttttatataa gaaagaaaca
    29521 cttggaatca ctcttttctt tccaagtatt tctttcttga ttttttgtct tttttcaaaa
    29581 tttttcgcta cgaaaaagaa agaatattta tatgaaattt ctatgaagtt gattgaattc
    29641 catgcgcttt tttagtatag ttaagtttgt gtaaaaatta tagaagagga agtattctat
    29701 gaataaatgg aaaaactggt tattgggatc cggacttgtc agtatcctgc tctttttatc
    29761 tggctgtgtg cggatggatt caaacggaaa ccctgacact tcaggaatcg tttatcgagt
    29821 actcgtccat cctatgggcc aagcgattac atatctagtt gagaatttca attggtctta
    29881 cggctgggca gttattgcaa tgactgtgat cgttcgtatc atcatcttgc cactaggtat
    29941 cagtcaatcg aaaaaaacaa tgatccaatc tgaaaaaatg caagcgttaa aaccacaagt
    30001 tgaagcagca caacaaaagc taaaagctgc aacaacacgt gaagaacaaa tggcagcaca
    30061 agcagaaatg cagcaagtat atcgcgaaaa cggattgagt atgaccggcg gaatcggctg
    30121 tctgcctcta ttgatccaaa tgccgatctt ctctgccttg tattttacgg ctcgctacac
    30181 agaaggtatc cgtgaatcat ctttttacgg catcgatcta ggctcaccaa gtatggtgct
    30241 tgtggccatt gcaggggtcg cttatctgct tcaaggatat atttcaacaa tcggtattcc
    30301 tgaagaacag aaaaagacga tgcgtacgat gctgatcgtc tctcctgcta tgatcgtctt
    30361 catgtcgatc agtgcgccag ccggcgttac actttactgg gtagtcggtg gtgtgttcag
    30421 ctgtctgcaa acattcatca caaacgttat gatgaaacca cgtttgaaag cgcaagtggc
    30481 agaagagttg aaacaaaacc cgccaaaaca ggtcgtcact ccaagaaaag acgtgacaga
    30541 atcagcaaag ccagctgaca acaaacaagc aacaaaacaa atcaaacaac caaaaaacag
    30601 tacaaataac aaaggccgca acgcaggaaa gcagcagaag cggaagtaga tagtaaataa
    30661 gagtgtaata aaaatgcatt aacagtttta gcaattaaaa atatcgacca agatttttga
    30721 aaaagaaatc tggtcgatat ttttatttaa gtgaatgtat aatttgaatc atcgattgat
    30781 cgctatctaa ttgcagcagc atctttttat gaagaagcaa ctttttctta tctatcaacg
    30841 tagacaggtg ttgctgtttc ttttaatagt gtacttaatt ccttaatatt ttgttttcct
    30901 tcttctaaca tccaactact cgctttttct tcgccttcgg taacaaaagg tgccactccc
    30961 ttcgcccatg ttgctcgccc acataagaca ccattgaatg tcgaacctga ttgttttgca
    31021 aaacgtaatg tatcttgaaa tagcgtagca ttgactcccg cacttaaaaa aataaacggt
    31081 tgttcagtca ctttcgattg ttcatagaaa tacgcggatg cctctgcttg actataacaa
    31141 gtctcttgcc catatccttc cacaaatctc atattcactg gcacttctac tttcaataca
    31201 tccacttgat aacgttcttt tgaaaattct cgcatggctt caataacttt atggggtttt
    31261 actttcgcat aggcaagaga ggacggatcg ccaatagttg catcataaga aaggatctct
    31321 aaaaagaaag gtatgccttc tgcttcgcat tctgaaccaa tccgttctat aactatttgt
    31381 ttttgcacat taatctctgc tggttcatcc acgtcatagt acaataaaaa tttacaagca
    31441 tccgcaccag cttcttttaa ccttttcacc gaccaaatat tcaataaatc aggtaatctt
    31501 cccggcattt tcgtatcata gccggttttt tcataagcta gcagcaagcc acattctttc
    31561 tctcgaagcg ctgctgcagg caggccgtat tctggatcaa gtaaaatagc tgaggcatag
    31621 ggagtcaggt attgagaaac catctgttta aattggatga tgtcttctga tgtagcttct
    31681 ttatattggt tgatcatttt cttcaaagca ccacgctggt cgattgctaa cgcgctaata
    31741 attccagctt catccgtcaa tcgcttcaaa cgttcctgct tttctatact catcttcttc
    31801 atagatcttc acctatttct ttagttaata ttgtggctat ttctgttttt gattttgctt
    31861 ttctcaactg ttctgtaacc tcttttcgca tcaatgatcg tgcaacgaga gacaataatt
    31921 tcaaatgagt cgttcctgct tctgaatctg gaataaataa cgcaaaaata aaatcaactg
    31981 actgtctatc taaactgttc cattcaattc ctttgttcag tgtgacgata atcatactag
    32041 gttcgttgat tgtttgagac ttcgcatgag ggatagcaaa acctgacatt attcctgtag
    32101 taccttcttt ttctctttcc agaagttttt gatatacttc tttttcgtca tgagcgattc
    32161 ctaaacagaa tgtttgcttt gctaaaaact taaatgcttc ttcttgacta tctaatggct
    32221 gttgtaaaaa aatgttctct tcatgaatga ttgtcatgat aagcctcctg ctcttccttc
    32281 ttcgataact gtacgatttg ctgctttatt atccatcttc ttgtcttcat cagttactct
    32341 tttcttttgg attaatccta gtatcactcc accaacgatc gaaccaataa gaatcgctgt
    32401 aacccattgg atttttcctg ttactacagg taatactaaa aaaccgccat gtggtgctgg
    32461 aacttggacg ccaaacaaat aagtcaagac agcagcaatt gatgagccta acatcatcgt
    32521 gggtaacact cggatcgggt ctttcgcagc aaacggaata gctccttccg taatatgagt
    32581 tgatcctaaa ataaaattaa ccaaacctgc attacgatca ttggtatcaa aatatttacc
    32641 aaacaataaa gtagcaaaac cagtaatcaa cggtggtgca atacaagctg ctgacacacc
    32701 agccataaaa gtcgtgtttc cttgtgccaa aagagctgtt cctgttacat atgccgcctt
    32761 attaatcggt cctcccatat caaacgcaca catgcaacca acaatcacgc caagaacaag
    32821 tggattcgaa tgttcaaatc cagctaaaaa gctcatcatt ccttcattta tagccttcat
    32881 tggacctgcc agcaaagaca tcacgatgcc aataaatgct acaccaataa ctggatataa
    32941 gaagattgcc ttcaagccat ctagttgttt aggtaaaatt gtcagtagct tttgcaaaaa
    33001 taagataaaa taacctgcta agaacccagc taaaatacct cctaaaaatc cagtaccgcc
    33061 attcatgacc acgattccac caacaaatcc aacgactaac cctggacgtt tcgcgatccc
    33121 ttctgcaata taagctgcta aaactggaac catcattccc attgctgcat cgccaaccgt
    33181 ttttaacatc gccgctgtag cattgtattg tgcatgatct ggatcaaaag aatagattcc
    33241 ccaaacggca aatgaaatag caatcaatac tccgccagct accacgaaag gcaacatatg
    33301 agagacacaa ttcatcaaat ttttatagat gctacgacca aaattagcac cactcatcac
    33361 ttctggttct aaattatcaa cagcttcatt atctgctaag acagtacctt ttcctgccaa
    33421 tgcttcgtca atcagccgtt gtgcatcctt gattcctgta ctgacagaaa catcaatgac
    33481 tcgttttccc gcaaatcttt ctgaatggac atctttgtct gcggcaataa tgacagcatc
    33541 tgcctgttga tttcttcagc cgttaattca ttttcgatcc caatttgtcc atgggtctct
    33601 actttgatcg tgatgccttt tttcctagct gcttgttcta atgcctcttg cgccatgtag
    33661 gtatgcgcaa ttcctgtcgg acaaccggtt gctgcaatta gttgatattt tcccatcttt
    33721 cgaacctctc ttccctgatt ttgatttgtt tacttaattc ctccacatct aaaaaatctg
    33781 ttaatccttt gcgaaaagcg gttgagctgc ctgctgctat gcttttacgt aacgtttcat
    33841 caagtggtcg tcgattcatg tatcctgcta aaaaagcgcc taacatggta tcacctgcac
    33901 atgctgtatt gacaactttt ccttttggcg agtttcctcg aaacaccctg tctttcgtaa
    33961 aaagaatcgc tcctttctca ccgagggata acaaaacatt ttctgcacct aactctacta
    34021 gtcgttgtcc ataaacaaag taatcttcat ctgtaaccat ttttcgtccg aaccaagaag
    34081 aaagttcttc ctcgtttggt ttcagcaaaa acggtcgata gggtaaacaa tccatgactt
    34141 cttgatcact actatcaata atcaaatgaa tccccttttc ctgacaaatc cgactgattt
    34201 ctattaaaac ctttggagac agccctcgtg gtaaactccc agaaacacaa agataatctt
    34261 ctgcttgtaa tgctcttatt tgatttaaaa aattatggac agctgtctgc ggtatttcag
    34321 gcccttgatt cactaatttg tattcttctc ccgtttgatt gacttgtgta aatacattga
    34381 tacgcgtcat accaggcact tcaataaact ctgtttggat ttgtttctgc tgtaaatagt
    34441 cttctatata ctttcctgtg aacccagcag aaaaacccaa tgccgtactt gggaaatcta
    34501 gcatttttaa tactagtgac acattcaccc cttttccgtt tgcctgaatg tcatcttctt
    34561 ttgtgcggtt taccataaaa ggatgcatct cctctgtttc aataaataga tcaattgcca
    34621 aattcatcgt gcatgtgtag atagacataa ccaacgacct ccttgactct attatgaaat
    34681 acgcgaaaga gaaaatgttt tcttaatcgt cccaacacaa agaaaacctt tacatttata
    34741 aactcgtcgt gctatttttt aactaacttt tggtgaaaga aggaaagaat atggatcaca
    34801 aattaagtga aacagaacag tacctctgga actttatcga acaccacatc ctagagatcc
    34861 ctaactactc catcgtgaaa ctaagtgaac aagcgaatgt ctcaacagcc accattgttc
    34921 ggacaatgaa aaagaaaggc tatgaagggt ttacttcgtt taagcatcat ctaaaagaag
    34981 gagaaaacaa gaacatcaat ttttctttct tagataaagt agacaaaggt atccgccgag
    35041 ccattctaaa aaatgaacaa gaagtggtac gtacgatcaa tatgttggaa atcggaaaca
    35101 ttgaggatgc gatacaaaga atcaaatttg ctgaccgggt ttttgtcttt gcccgaggat
    35161 tctcagaaat gattggccaa gaaatgctgg taaaacttca attaacggga aaaaattgtc
    35221 aaatgcatac tgatccagaa attatcaaaa gcatcagtcg gaaactgact aagaaagaca
    35281 gtgttatttt tgtttctttg aacggcgaga caaaagaact cgtcaccgcc gcaaagaatt
    35341 gctatgatgc agagattggt tcaattctag ttacagccaa tcattcctct acactcaaag
    35401 attattgcga gataaatctc gtaggtttca aatcagaagg ttcgtatttc cctaattatg
    35461 aagtacggtc acgcttgcct ctccagatta tcgcgagaat tctgcttgac gcctatgtat
    35521 tgcgtatggg ggagaattga gaagcagttt attgtggttg gttacagttt aaaacatcac
    35581 atttttttat tctttatatt gtagggactc acggaaactt tttatgtata aggttattca
    35641 caatccacaa ggttacttta agtaacaaaa aaataagtga aaatacttaa aactaaaaaa
    35701 tatcttttta atcaataaaa aaa
//
LOCUS       DAZABD010000024        35216 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000024 DAZABD010000000
VERSION     DAZABD010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35216
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.024"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(211..1059)
                     /locus_tag="ACHALG_001243"
     CDS             complement(211..1059)
                     /locus_tag="ACHALG_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFN2586666.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1283..3223)
                     /gene="thrS"
                     /locus_tag="ACHALG_001244"
     CDS             complement(1283..3223)
                     /gene="thrS"
                     /locus_tag="ACHALG_001244"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFN2586667.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3560..3739)
                     /locus_tag="ACHALG_001245"
     CDS             complement(3560..3739)
                     /locus_tag="ACHALG_001245"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HFN2586668.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3786..5609)
                     /locus_tag="ACHALG_001246"
     CDS             complement(3786..5609)
                     /locus_tag="ACHALG_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFN2586669.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5887..8166
                     /gene="helD"
                     /locus_tag="ACHALG_001247"
     CDS             5887..8166
                     /gene="helD"
                     /locus_tag="ACHALG_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase recycling motor HelD"
                     /protein_id="HFN2586670.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8259..8906)
                     /locus_tag="ACHALG_001248"
     CDS             complement(8259..8906)
                     /locus_tag="ACHALG_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2586671.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(9085..9804)
                     /locus_tag="ACHALG_001249"
     CDS             complement(9085..9804)
                     /locus_tag="ACHALG_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN2586672.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9935..11086)
                     /locus_tag="ACHALG_001250"
     CDS             complement(9935..11086)
                     /locus_tag="ACHALG_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HFN2586673.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11303..12043)
                     /locus_tag="ACHALG_001251"
     CDS             complement(11303..12043)
                     /locus_tag="ACHALG_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFN2586674.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(12047..12370)
                     /gene="rsfS"
                     /locus_tag="ACHALG_001252"
     CDS             complement(12047..12370)
                     /gene="rsfS"
                     /locus_tag="ACHALG_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFN2586675.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12427..13020)
                     /gene="yqeK"
                     /locus_tag="ACHALG_001253"
     CDS             complement(12427..13020)
                     /gene="yqeK"
                     /locus_tag="ACHALG_001253"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HFN2586676.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(13004..13654)
                     /locus_tag="ACHALG_001254"
     CDS             complement(13004..13654)
                     /locus_tag="ACHALG_001254"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFN2586677.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13664..13975)
                     /gene="yhbY"
                     /locus_tag="ACHALG_001255"
     CDS             complement(13664..13975)
                     /gene="yhbY"
                     /locus_tag="ACHALG_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFN2586678.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13991..15100)
                     /gene="yqeH"
                     /locus_tag="ACHALG_001256"
     CDS             complement(13991..15100)
                     /gene="yqeH"
                     /locus_tag="ACHALG_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HFN2586679.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNEIQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(15097..15624)
                     /locus_tag="ACHALG_001257"
     CDS             complement(15097..15624)
                     /locus_tag="ACHALG_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HFN2586680.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15804..16589)
                     /locus_tag="ACHALG_001258"
     CDS             complement(15804..16589)
                     /locus_tag="ACHALG_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFN2586681.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16582..17451)
                     /gene="accD"
                     /locus_tag="ACHALG_001259"
     CDS             complement(16582..17451)
                     /gene="accD"
                     /locus_tag="ACHALG_001259"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFN2586682.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17452..18831)
                     /locus_tag="ACHALG_001260"
     CDS             complement(17452..18831)
                     /locus_tag="ACHALG_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFN2586683.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18839..19258)
                     /gene="fabZ"
                     /locus_tag="ACHALG_001261"
     CDS             complement(18839..19258)
                     /gene="fabZ"
                     /locus_tag="ACHALG_001261"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFN2586684.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19283..19759)
                     /gene="accB"
                     /locus_tag="ACHALG_001262"
     CDS             complement(19283..19759)
                     /gene="accB"
                     /locus_tag="ACHALG_001262"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFN2586685.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVISEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19762..20997)
                     /gene="fabF"
                     /locus_tag="ACHALG_001263"
     CDS             complement(19762..20997)
                     /gene="fabF"
                     /locus_tag="ACHALG_001263"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFN2586686.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(21016..21753)
                     /gene="fabG"
                     /locus_tag="ACHALG_001264"
     CDS             complement(21016..21753)
                     /gene="fabG"
                     /locus_tag="ACHALG_001264"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HFN2586687.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21765..22682)
                     /gene="fabD"
                     /locus_tag="ACHALG_001265"
     CDS             complement(21765..22682)
                     /gene="fabD"
                     /locus_tag="ACHALG_001265"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFN2586688.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEATPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22789..23013)
                     /locus_tag="ACHALG_001266"
     CDS             complement(22789..23013)
                     /locus_tag="ACHALG_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFN2586689.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(23066..24028)
                     /locus_tag="ACHALG_001267"
     CDS             complement(23066..24028)
                     /locus_tag="ACHALG_001267"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFN2586690.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(24038..24478)
                     /locus_tag="ACHALG_001268"
     CDS             complement(24038..24478)
                     /locus_tag="ACHALG_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFN2586691.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            24784..25749
                     /locus_tag="ACHALG_001269"
     CDS             24784..25749
                     /locus_tag="ACHALG_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFN2586692.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(25806..26036)
                     /locus_tag="ACHALG_001270"
     CDS             complement(25806..26036)
                     /locus_tag="ACHALG_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HFN2586693.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(26122..27009)
                     /locus_tag="ACHALG_001271"
     CDS             complement(26122..27009)
                     /locus_tag="ACHALG_001271"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFN2586694.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(27098..28321)
                     /locus_tag="ACHALG_001272"
     CDS             complement(27098..28321)
                     /locus_tag="ACHALG_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFN2586695.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(28338..29384)
                     /locus_tag="ACHALG_001273"
     CDS             complement(28338..29384)
                     /locus_tag="ACHALG_001273"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFN2586696.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            complement(29548..31275)
                     /gene="ptsP"
                     /locus_tag="ACHALG_001274"
     CDS             complement(29548..31275)
                     /gene="ptsP"
                     /locus_tag="ACHALG_001274"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFN2586697.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(31275..31541)
                     /locus_tag="ACHALG_001275"
     CDS             complement(31275..31541)
                     /locus_tag="ACHALG_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HFN2586698.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            32020..34254
                     /locus_tag="ACHALG_001276"
     CDS             32020..34254
                     /locus_tag="ACHALG_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFN2586699.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            34402..34722
                     /locus_tag="ACHALG_001277"
     CDS             34402..34722
                     /locus_tag="ACHALG_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFN2586700.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(34927..35151)
                     /locus_tag="ACHALG_001278"
     CDS             complement(34927..35151)
                     /locus_tag="ACHALG_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HFN2586701.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
BASE COUNT         9930 a         7599 c         6108 g        11579 t
ORIGIN      
        1 aaattcttta cctaatttca agataaaatc aactttttgt ttcacagcaa tcacttaaag
       61 ttacatatcc aactattttt ttcattaaaa aataaactta tttttgaaaa ttatgttttc
      121 ttaagtcgtg aaacactttg atggatcatt tatttgtgga aaactacgtt ttgttattct
      181 aaaaaactta tataaaaaat agtaaggttt ttatttcgaa tccataaaat attcttggat
      241 tgcttctcgt gtttttttca ctttttcgct aagcttttcg tttttagcca tatagaaata
      301 aaaaatgata tcgatcgtag caaattgggc caaaagtgaa ttagttgccg cacttcgatt
      361 cgtgatttct tttggtctag atgtttgtac gagtacatcg gctatcttgc taagcgggtt
      421 atttccaaat tgcgtcaacg caatgatttc gatattcatg cttttcgcca gctcggctaa
      481 tgcgactaca tctgcacttc tacctgagtt tgaaacaagc caaagcactt tctttttctc
      541 attgctgaca agttgcggga gcaagacgtg gatatctttt tcaaaaatga ttggcttacc
      601 gacacgagac catttttgca gaatatcttc tactactaaa gaagaggcgc caaccccata
      661 taaaaaaaca gtctctgccg attccaaaca ctctacgact ttttggatcg tttcttcttc
      721 tagaatatct actgtttctt ttatcgctac ttgtgcattg ttaccaagtt tgtttttgat
      781 agagtaaaag gactcatttg cttctatatc actaaaacct tcatcgattc cagtctgtag
      841 ttctgttgaa atcgtgattt tgaagtccgt cagactttga aaaccgattt ttttggaaaa
      901 acgtacaact gtcggcgcac tcaacccgga agcttctgct aattcattag ctgtcattgt
      961 agggaccttc tcaatattac cgataatgta gttggccaac tttttttctg ctgaagaata
     1021 atcttccatc atactttcaa tacgactaat tacgctcata agttcctcca cccttttaat
     1081 gatagtctat gtaagtatac catatcagac ccttattcac ggtcgtatac tggataaaaa
     1141 acaaaaaaac aatccgccca acagaatatc ttatcccatc aatcccacag attctattga
     1201 gcatctgtgt ctttgaggaa tacctgttat ctattgttcg gattgctttc tatctactac
     1261 ttttttacag gaaatttacc tattagtttt ctctgctgta atttttgact tctgcttcca
     1321 tagcatcaat aaagatattc aaatcttcta cagctgtttc tttacttcca taacgacgta
     1381 cgttcactgt tgcatcttcc aattccttgt ctccaacaac taattggtaa gggatctttt
     1441 gtgtttgaga ggcacgtatc ttatagccca ttttttcatt acgatcatca acttctacac
     1501 gcaacccctt catttgcaaa cgttctttga tttcataagc ataatcgcca tgtgcatcaa
     1561 cagaaacagg gatgattgtt gcttgtatag gtgccaacca tgttggaaaa gcgcctttgt
     1621 atacttctgt taaataagcg acaaaacgtt ccattgtaga aacaattccg cggtggataa
     1681 ctactggacg atgcgtattt tccccgtctt ctccaacata tgtcaaatca aagcgctctg
     1741 gcaataagaa gtctaattgt atagttgata atgtttcttc tgtaccaaga gctgttttga
     1801 cttgaacatc tagttttggt ccgtaaaagg ctgcctcgcc ttctgcttca aagtaatcta
     1861 gttctaattc atccatagcg gctttcagca ttgcttgggc tttttcccac attgcgtcat
     1921 catcaaaata tttatctgta ttgtttggat cgcgatagct caagcggaaa cgataatctg
     1981 taatattgaa gtcagcatat actgccacca tcaattcaag cgtacgttta aattcatctt
     2041 tgatttgatc aggacgaaca aatgtatgac catcattcaa cgtcatttct cgtacacgtt
     2101 gtaaacctga caaagcacct gatttttcat agcgatgcat cattcctaat tcagcgatac
     2161 gaattggtaa ttcacgataa gaatggatcg tgtttttgta gaccatcatg tgatgagggc
     2221 agttcattgg gcgaagaaca agcatttcgc catctcccat atccattggc gggaacatgt
     2281 cttcttgata atgatcccag tgtccagatg ttttgtacaa ctcaacatct cccattattg
     2341 gtgtatatac atgttggtaa cctagactaa cttctttgtc tacgatataa cgttcgatcg
     2401 tgcgtcggat tgttgcccct tttggaagcc agaatggcaa accagaacct acttctgggg
     2461 aaaccatgaa caagtcaagt tcttttccta gtttacgatg gtctctttct tttgcttctt
     2521 cacggagacg gatatattct ttcaaagctt tcttgtcaaa aaaggctgtc ccgtatacac
     2581 gttgcatcat ttgattgttt gagtttccac gccaataagc acctgccact gataataatt
     2641 taaatacttg tatacggcca gtggatggaa catgtggtcc acgacacaaa tcgatgaatt
     2701 cgccttgttg ataagcagta atcacttctt cttctggcaa ttcactaatc agttctactt
     2761 tgtacggatc acttgcaaaa atttctaatg cttcctgttt tgaaagaaca cggcgttcga
     2821 tcggcagatt ttctttgacg attttcatca tttctgcttc gattgccggc aaatcttctg
     2881 cagttacttg gttttgtcca ttatctgtat cgtagtaaaa ccctgaatcg atagctggtc
     2941 caacgccaaa gtggatattt ggatagaggc gacgcatggc ttgggccatc aaatgagctg
     3001 ctgaatgacg gactaagcca agtgcatctt catggtctgg tgtgatgatc tcaatacttg
     3061 catcttcctc gattggacga gtcaaatcaa cgagttttcc gttcacttta ccagctaaag
     3121 ctttttttgc taagcttttt gaaatgcttt cagcaattgc caatgttgtt gttccagact
     3181 caaattcttt gactgcacca tctggaaaag taatttttat cataatagtt tcctccttga
     3241 aatgttttga ttgatcagat catcaagctg aaaaacaaaa aaatcctagc atctcttgaa
     3301 aagagatact aggacgaaaa gttcgtggtt ccacctaatg ttcaaacgca aatgcgttcc
     3361 tcaagcgttg taacggtccg accgcccttg cttcaacaag ggtttcgaaa gtggtcatcc
     3421 tttggttttc tcctggaaaa gtttcagcct agcttttcct ctcttatcga agactctcaa
     3481 attcagttgg ctttctcatc aatcaattat gtgtttattt aaccactaat aaatagaaaa
     3541 atcaagtggg aaactaaact tattttttcc attccccatt tacaccgtat gttccttttt
     3601 tcatctcatt gatgatgaca tggatgtgtt cttttggtgc ctgcgtgttt ttatgaacag
     3661 cttccgtgat atctttcatc atattttcta attgttccgg actgcgacct tctactaatt
     3721 cgacatgtac gaatggcata atgctctctc ctctttctga tcgttctttt ttcaactcga
     3781 ttctatcata ctatgtttgt cttgggtact gaatcaggaa aaatggctgg atcttttttt
     3841 gctctcaagg cactcaaaat agagaccgta tccacgactt cttgcaaaac agctccgaat
     3901 aatgcgggga ttagtcccgt acttgcaatt aacatcaaaa aaatacaaat aaagatgcca
     3961 atcagtacag actgtttcgc tattctcatc gtatctcttg aaatcactac tgcttttgct
     4021 acacgcaaga gatcatcttt caagatgacc gcatctgcac tttcactagc agctgtagaa
     4081 ccatgagccc ccattgcaat accgatatct gcagcagcaa gtgctggtgc gtcattcacg
     4141 ccgtctccta ccatcgcaac aggtctttga tctttaggga tactttctaa aatggtcagt
     4201 ttatcttgag gcaagctacg ggcatgtaca gaatctaatt ggactttttc tgcaatcgtt
     4261 tgagcaatgg cagatccatc cccggtgatc atcatcaaat ttttcacatc aagttttctt
     4321 agctccttga ttgtctttgc tgcttctggg cgaatctgat ccgaaaatag gatcgttcct
     4381 gcaaaagatc gatcaattgt tacatagatt gctgtattgg acgtttctat ttcttcagct
     4441 gaaacaaaag aggcacgtcc aatctttact tcatgattat tgactactgc ttggattcct
     4501 tgaccggtca cttctttcag ttgactaaca ggatacagtt ggctgttttt cacgttgttc
     4561 actaacgaac gagctaagat atggctagat tcctgttctg cactagctgc atacagaagt
     4621 agctcttctt tttttattcc attgactggc tgaatcgcct ctacttccaa tatccctttg
     4681 gtaagcgttc ctgttttatc aaaagcaatc gtttttgtac gtgcgagctt ttcaatcgtt
     4741 gttccagttt ttacaacgat cccgttttta cttgaacggc tcattcctgc aactaaagca
     4801 atgggcgcag ctagaatcag tggacaaggt gatgccacta ccagtacctc agcaaatcgt
     4861 aaagggtctt ttgagaacca ccaagaaata ccaccgatca aataagcaat caacgtgaaa
     4921 gggactgcat aacgatcagc taaacggaca aatcgggcag gcttttcttt tgattcttcg
     4981 actagtttaa taatcatttg atactggctg tctgcggcac gtttttcagc gatcatttgg
     5041 atcgatccgt ctccatttaa agaccctgac ataatctgat ctccaggttt cttggtttcc
     5101 gggcgggact cacctgtcaa agaagattca tcgaccgaag attctccttt ggtcactcga
     5161 ccatctgcgg gtacaagttc gccaggcttg actagtaact ggtcatcaat atttatttca
     5221 tcgattacta tatcctgcaa ttttccttct ttgaatcgat gggcaattct tggggtgtta
     5281 tctaacaaag actgcagttc tttacccgct cgtttattcg cgtaatcctc tagactatct
     5341 ccgcccgtaa gcatgatcaa tatcatcaaa ctagcccagt attctttgac ggctaatgtt
     5401 gcaaggattg cggtaatcgc taatacatct acgccatatc ttcctgaacg taacgtcttg
     5461 atcatttcca aaaacatcaa gaaggcgaga atccctccag taaatgcgac aatcgcaaat
     5521 gcaagggccg gctggtgaaa aacaaattca cttaataaag ctatcccacc aataagcaaa
     5581 gtaagtaaaa attttttgaa atgtgtcatt gttttttctc ctcttttatg tgacttagag
     5641 aggttagtaa taaatttcca gctgcctctt tcatcttaag catagtcctt gactagcact
     5701 ttttaaaaca ataggcctta attgaaaaca actatcacta tcagcgtttc caaaattcat
     5761 cagttctaca aacaaaaata ttcagcaagg attacttttt cgattcatca gttaaaaagt
     5821 accttttttc gaaaaactat gctatacttt taattcgtta tttacggcgg aaggagcaaa
     5881 catgcgatga atgagaaaca actagaacaa acgcacgtgg atgaaacgat cgaactaatc
     5941 cagatcgaac aaaatatgct ggatacaaaa caacaagaat tgactcagga aatgaatcat
     6001 tcgacaaaag acacagctaa tcacaagatt cgagctggtt ccaatgaagc tttttatgaa
     6061 tctgctgttg aatatcgcca acatgagcag gaattgcttt tgaaatacca tactcttgaa
     6121 gcacagcaaa aacgcctgca aacgttgtct gtcatgaaag atagtcctta ttttgcccgt
     6181 atcgacttta aagaagaccg tgaaacagaa acattatatc taggtatcgc ttctttaaga
     6241 gatctggcag aagaaacaat cgttattgac tggcgcgcac caattgccaa tctttattat
     6301 gaaggtgaga ttggtcccgc attttatgaa actgaaacag atagaataga tgtcgatctg
     6361 cagttgaaac gacaatttaa aatccaagaa ggacgtattt tatctatggt agatacgtcg
     6421 gaagtaatca atgatgactt tttgctagag atattggatg aatcatcaag caaccatatg
     6481 aaaaacatcg tctctacgat acaaaaagca caaaatgcaa tcattcggga cacaacaagt
     6541 aaagtaatgc tgattgaagg aatcgccgga agtggaaaga catccgcttt gcttcaacgt
     6601 attgcttttc tgttgtatca taatcgcaag tggctcgagg ctgaaaacgt tcttctattt
     6661 tcacccaacc atttgttttc tgattacatc tcgactgtat tgccttcatt aggtgaaagc
     6721 ggcgtgccta cacagacatt caaaaattat ttggtgcagc tgctgccaga gttttcttta
     6781 ctagatgaac agcaacaaga aagtggtttt ttgctagggg aaaaagatcc gatacaagtg
     6841 atgaaaagcg gattgacctt agtcgatcag attgatcgct atatccaagg catcacaaac
     6901 tacggtccct tgttccgtga tatgaaaata aatggccgta cgatcctatc aaaggaatcg
     6961 attcgccaat ggtataaaga aacaaatgaa caattacctt tacatcatag actttctcta
     7021 ttacaaacga agctcctgaa aaaattaggc ggtctgcaaa aagacgaaac acgtcagcaa
     7081 tgggtcaaag atctggcgga agaacagctc caagaactat acgcaacaga tccaaatctt
     7141 gaatatacag aacaaaagga gcgttcatta cgtaaaaaac tggccaacca aatcgtacgt
     7201 aaaagattcc gtcgaatcca tcgagggatc atgaattatc aatttgtcaa tataacgaaa
     7261 caatatctcc actttctaca aactgtgcca ctttctcttc taacacgaca taaaatcagc
     7321 gagaccaggt ggcaggaaca tttgacaact gttcgtacac acttgcgtca aagagaattg
     7381 cgtcaagagg atgctgtact attcttttta ctgatgcgcc gaacatatcc agtgacggtt
     7441 acacacaaag ctcgctatat ctttattgat gaaatgcagg atttcgcacc atcacaagtg
     7501 gcattgctgc aagccttgtt cccaaaagca aatttgactt tctgtggaga tctaaatcaa
     7561 aatgtctttg gaaatgaaac gatcgttgga tcattggaag accttttcgt ggaccgtgaa
     7621 gtcactcgtt tccaattaac gacaagctat cgctccacaa aagaaatcac tgattttgct
     7681 aatcatttta ttgtgaaaga acatcaagta gaaacaacag caagaaaagg agaccttcct
     7741 ttgctcatcc aaggaacaac agatgatatc aaattgaatt ggttagcaga taccattcga
     7801 acgactcagg gaaaagcccg gtattggcgt acagcaatca tagctaaaac acaagaagaa
     7861 tgcgaagctc tttatgctca actgccagac gagttgaaac aacaagtcca gctgatttta
     7921 aatgagagtg actttatgaa gcgttccatt attttacttc cagccttttt agcaaaaggt
     7981 ctggagtttg accgagtttt cttgtgggat gtagatgcag aacatttttc ttctcagcaa
     8041 gacaaattga tcctttacac catgtgtaca cgtgcgatgc atgaattgac cctttttacc
     8101 tccaataatg aaagtccttt gttaaacaat atggatattt catcttatga gaaaaaagta
     8161 ctgtaagtaa aaagacaact gctatcatta atgttagcag ttgtcttttt atttacaata
     8221 ataattgcag aaaaagtaat tcaatcattt aaggtcattt attctatata taaatccaaa
     8281 tctgtttcag gatattttaa tttgacaagc gaaataatca ttttcagttc ttccatgttt
     8341 tctggatatt ctgggtcaaa taccttgcta tgacggccaa tcccgccatt gtcgatcaat
     8401 cggtaaattc gattccattc aatgaattgg tcaattgatt ggaaattgct gattggatag
     8461 ccgccacaat ccaaaatctc tgatacacga aatgctaggt atgcactatc gatcacttta
     8521 ttttttttcg tgtctatctt ctcatttctt tcgataagaa ttttttcagg acgatgtttc
     8581 aagctccact ctaatagtag aaagaaagcc tccatccgat actcttcttc aaataaccga
     8641 aaggctgaat cagaacgacg aaaaagcagc ggaatcagat aattttgtct ataaatcagc
     8701 attggtgcgc ggtccaccat acactctaaa taacggttca gataccctgt accgacatag
     8761 tttcctaatc gatagccttc aacgccgaat cttgcgtatt tttgcgctaa ttttgaacaa
     8821 tcagttacgt tcacagctaa ctcttctact ttgcggtaac cgcttaagat acgttcaaaa
     8881 ggttctaaag tgatttttgc ttccatattc ttccccgtcc atcttccaca tacttcatat
     8941 catttcacat gatacttatg ctctcatcat accacaggtt gaattagaaa agaaaataaa
     9001 tcacacaaaa aagcaagaca atctgttagt gagattgtct tgcttttcga aaaagactga
     9061 atacttttcg ttaaaactgg ttgcttagtc ttctaatgcc acattatgga acacatgctg
     9121 tacatcttct agatcttcca gtgcatcaat cattttttca aatgtttctt gatcttctgg
     9181 agatagtgtt acctcgtttt gagggatcat ttggatctca gcgattgaaa aatcagtgat
     9241 tcctttttct tttaatgctt cttgtatact atggaaatcc gctacttcac cgtaaacgat
     9301 gatttgtcct tcttcttcca ttacatcgcg aacatcgatg tctttttcca ttaggtattc
     9361 aaggatctcg tcagcgtctt ctcctgcaaa agcaaagatt gctgtattgt caaacatgta
     9421 agaaaccgct ccggaaacac ccatgttccc gccatttttc ccaaatgctg cccgtacgtc
     9481 tgctgctgtt cgattcacat tgttcgtcaa tgtatcaaca atcaccattg agccgcttgg
     9541 tccaaatcct tcatagcgca attcagagta tgtttcatct cctgcacctt ttgctttttc
     9601 gattgctcgg tcaataatat gttttggtac attataagtt ttagcgcgat caatgacgaa
     9661 acgtagtttt tggtttgcat gcggatcagg atcacctgat tttgctgccg cgtagatttc
     9721 aatcccaaat tttgcataaa tccggctatt attagcatct ttagcagttt tctttgctac
     9781 gatatttgcc catttacgtc ccatattttc acttcgcttt ctgatttgat agtttcataa
     9841 cgcaaaaacg tcctgttcca ttatacttgt gaatcacgat tctgtgaagc tttttttctt
     9901 tcttccttca ttcttctcat ttttagtcaa ttatctatac tcggattggg atttgcccga
     9961 aattctgttc cggaatattg cctcccaatt gatagatttg atcacttcta actaacagtt
    10021 ccgcttcttg tgcctctttt tttccgattc gggaataaat agaaatctct tttttttctt
    10081 ttaagaatct tcttccagca tcggtaaatc caagtagtcg aagataattt ttattttgtt
    10141 ggacaggaat ctcttgcttt ttgatattca gcaagacata acaagataat cgttggatcc
    10201 gtgtccatgt atatcttttt gtttttacct ttgacacaaa atcctcaaaa ctatcagcag
    10261 ttgcagctgc tgttttcatg cgcgattcta ttccttcggt catctgatag atatctgtca
    10321 gttccagcaa agtagaagat aacaaccgat aacgcaaata cggccaataa gattcccaag
    10381 tctgcagcga acaagttgaa agatcttctt ctgtctgagg cggcacaagt ccagctattc
    10441 tttttccttc ttctaacgca tggcgaatgg ctgttgcact ggctatcctc tgatctgtga
    10501 tgattttttc atgatagcct gcacttttac gtcggatcgg atgtaattcc atagggtgga
    10561 catacttggc attttccttt gcataactta atcccaagat atgatttggc gaagaaaaat
    10621 caagcgtaat atcgggatac agactgcgaa aaacctcagt cattttttgc ggataactat
    10681 acgtttgatc agacagttct tgaaatgcct cttctatcct atcttgattc tcttggacaa
    10741 agcgtccgaa actgctgtaa tcaaacgaag tctcttcttc tgttccaaaa cacaaactgt
    10801 cacagtccag tgtttgcaag atttttactg cacctttagc aaaatagtca gctgactgaa
    10861 ccgcccattc gacgggaagc tccactacta aatccacacc attttgtaaa gcttcattcg
    10921 cgcgatgcca tttatcgatg atagccggtt cgcctctttg caaaaaattc ccgctcatta
    10981 cagcaatgac gacctctgct cctgttattt ttcgtgccat ttccacatga tagcgatgtc
    11041 cattgtgaaa aggattgtac tctacgataa tcccgcagct tttcatgcgc atcctccttc
    11101 atagttatcg ataaaaaaga aatagagaca agtaaaaaaa ggcaaaaatc ggaaaaaata
    11161 cccgaactat ataaactatt ctcgcaaaca caatacttct gcgacgaaaa gctgcactgc
    11221 aagagcaatg cctatatcgt tcggatatat aatttatgtt gaagatttat tcttttcttg
    11281 ttaactcgag caggcaaaac cgctagtctt tgtatgctac aaagaaccag cgctttgatt
    11341 tctctgatgc tgcttcgtct gtaaaatcag aaaatgcttc gactttcgaa aagccgctgt
    11401 tttccagcat tcttaaataa ctttccaatg gataggtacg ctcttgatgt agttcatctt
    11461 cacgaataaa tcgaccagac ccttcttgtt cttcaacgaa aaaggtaaga aaatgttcga
    11521 tgctgtgttt ctcgcttcct ggataactat cccacaaaaa agcaaaatta tccccttgat
    11581 aatgatagct atattctgga aaaacctcat ctacttgata ggtggagtgt acgtcaaata
    11641 aaaatcggcc attttcttct aacgcttgat agacttcatc aaacacttgc tgtacctctt
    11701 gtctattttt catatagcac agcgaatcag aaaaacaggt aattgcctga tattgtccga
    11761 tttctgataa atcgagcata ttgccttcca caaattggat cgatgcgtct tcttcatatg
    11821 ctcgctggct ggcaatcatc aacatctctt ctgaaaggtc taaccctgtc actttatatc
    11881 catcttttgc aaaggcaacc gctaatgctc cagtaccgca tgccagttcc aaaatatttt
    11941 ttgtgtcttc ttgtaaatga cgtttcgaaa aagctaacca ttgctgataa agtgtttcgt
    12001 ccatcacttc atcgtaaatg aaagcaaatg tttcataagc catatgttaa tccaaccatt
    12061 cactaagatc gactagcgga gcatctgacc atagtttttc cagattatag aaatttcgtt
    12121 ctggcgcatg gaaaacatgt acgatcacat ctccaagatc gatcaatacc cattttccgc
    12181 cttcttttcc ttcaatacgt tttacttcat atttgttttc ttcttcttta tcaatgattt
    12241 cttctgtaat tgcgttgatt tgacgttcgc tatttgctga acagatcatg aaataatctg
    12301 ccaacaagga aacatttcga acgtctaaag cgataatgtc ttctgcccgt tttgaatctg
    12361 ctgcttcaat cgcaattttt aattgttctt tactatctat gatgatttcc tcctaatatg
    12421 agcagcttat ttttttgcta cccagtaatt atatgtttcg attgtttttg gataaatctg
    12481 ttgttcttgc gcaagcaaat gggccaatgt atgttttgtt tcaaatgcta cagcttcgtc
    12541 tagatcagcc catgcaatgg ctcgtgcatc ttgtacccca ggaaaatctc gacctggttc
    12601 aatatagtct gctacataaa tgattttgtc aaggagactc atcttagcag ccccagtcgt
    12661 atgcacccgg attgcatgaa gaatctctgc atcggtgatt cctaattccc gctctacaaa
    12721 cgaagcgcca acaagaccat gccagattgc attattataa ttcaataact ccggatcaaa
    12781 accgtctctt acgattacca ttttgaactc ctcatctggt cgttcttttg cataatcatg
    12841 tgtcaatgca gcaatactag ctttttcagg agaagcaccg tattttttgg caagagccac
    12901 ggctgtttct tctactccaa gcacatgctt gaagcgttgt tcgctcattc tcatctgtat
    12961 tttttgcatc aggttttctc tagaataagc agtatattca ttactatagt ccatattcat
    13021 acaacccttc attttgaata taatcaatta ctttatctgg aacaaggtag cggatagaac
    13081 agccttcttt gatcttttga cgtattttcg tggaactgat gtcgatctca ggtacatcta
    13141 cccaaattac aggatatgga gtatccgttg tgtatcctgg tcgacgtata cccacaaagt
    13201 tgaccataga agtcagctca tcgattttat accattttgg caaatattct accatatccc
    13261 caccgataat aaagtaataa tctgtatccg gattgttttg cgtcaattcc ttcatagtat
    13321 cataggtgta acttttccca ccacgagcta attcaatcgt ttcaatctgc aaaaaaggat
    13381 tgtcttccac cgctaattcc aacatgttga gtcgatgttt tgcatcaatc gtctgtttct
    13441 catcaacatg aggtggctga taagaaggca tcaaaaatac gcggtcaaga cctaaattcc
    13501 ggccagcttg ttcagccatc actaaatgtg caaggtgaac gggattgaat gttccgccca
    13561 agatccctac ttgctttttc ttctctaaga attgaggcat ttcttcagga aataattgtg
    13621 aacgaacaaa tgtcttagct tgcgtattca tcatggtcac tccttatatt gcttttactt
    13681 cttttgatag cctttgatac ttttcttttg tggaaggttt gtataataca ataacacgtc
    13741 caatgatttg gacgatttcg caatctattt ctttttctaa tacttgagct gcttcttctg
    13801 caatctcatc tgtattttga agtaacgata ctttgatcag ttctcttttt tctaaagctt
    13861 cttcaatttg aacgatcatt gctgaattga tcccaccttt tccgatttga aaaatcggct
    13921 gaagatgatg cgctttgcta cgtaaaaaac gcttttgttt tcctcttaaa ctcataattt
    13981 tccttcttcc ttaaatcaat gctttgcgtt tcaatacatc tacacccttt ggcgcccagc
    14041 ccgcaattac tactggcgct gttacagtga tccagcctag accggcaaaa acgatgtctg
    14101 ttttttcttt cacagaaaac tcaaaacgaa caagctccgg aaatgcctcg acttcatcag
    14161 gacgaggagg ctgtaataat ccacctacat gtttttgata aaattcgtct gctttttcca
    14221 gtttcgttcg atgaatattt acatcatttg acacataagc aataaatgaa gcacgattgc
    14281 cgctgataaa atcaaatctt gcaagtccgc ccaagaaaag tgtttgccct tcattcagct
    14341 gataaacttt aggtttgatt tcttttaacg gtgcaatgat ccgcaaatct tttttaccta
    14401 agtagtgcgc catttgatgg cgatggataa ttcccggtgt atcaatcaaa aaatgcccgt
    14461 catctaaagg aatctcgatt ttatccaagg tcgttcctgg aaactgcgaa gttgtgatta
    14521 catcttgtac tcccgctgta tttttgatga tttgattgat aagcgtcgat tttccgacat
    14581 ttgttactcc gacgatatag acatcccgtc catcgcggta ttcttcgatt ttttctaata
    14641 gttccgtcat ctcatgagat ttcttagcac tagttaataa tacatctact ggacgaattc
    14701 ctgcttcgta agcacgttcc ttcatccatt gtgttaattt agtttttttc aatgatttag
    14761 gtaatatatc cactttgttc ccaactaaaa gtactggatt gtcaccgaca aaacggtgta
    14821 gtcctgggat tagtgaacca ttgaagtcaa aaatatccac cacattgacg atcaatgcat
    14881 ctttttgccc taattcattc agtaagcgca aaaagtcgtc atccgtcaat gagacatctt
    14941 ggatctcatt ataatggcgt aatctgaaac aacgctgaca atagacttcc ccggaagcta
    15001 aacctttttc caaagccgtt tgcggcgtgt atcctaattc accgggtttg tttgtttgta
    15061 taacggcacc acagccgata cattgtaatt cttctgtcat tcgattccac ctcgccagcc
    15121 catatcaggg tgttttgcta atagataggc cataattctt ttttcccaaa aacgattaaa
    15181 ttgtgtcttc caactatctg ttgcgataat gggttttaca aggatgctac gtatccctgc
    15241 acgattggct ccacgtatat cagtcataat ctgatcgcct accattacta tctcatcatc
    15301 tgctaaatcc aattgtttct ttgctatgtt gatccctctt gctaaaggtt tcattgctcg
    15361 tgcaacatac aaaagatcaa atttttcaat cgctcgcgcg acacgttttg aattattgtt
    15421 tgaaacaaca acaacaggga ttcctgcatt tttcatttct aaaatccaag taagcaactc
    15481 ttctgttcca tctggattat tccatgcaat caacgtattg tctaaatcgg tcaatactgc
    15541 tttgatgcct agattcttta attgggcagg tgtaatctga tagatggctt ctaccatcca
    15601 tgttggtttg taatttgaaa acatgctgtc tcctcatctg caaaaaatga aggagcacat
    15661 aaagtactcc tcaaccccgt attatactgc atatcagcaa aaaaaactac ctctatactc
    15721 gttcgtcttt cagaaaaagc atttctttac aaaatgacca aaaataaaga ttgatcacag
    15781 aaaaatttag aaacaaaaaa caactaatat tggcggaacc tcttgtatcg tttttccagt
    15841 aatttatgct gatctaatcg aacaagttcc gtcaatcttt caatcaacgc ttttttcata
    15901 atgtgtgcga ctttatatcg tggcaactgc tcgccgttga acgtttcagg aataactcga
    15961 tcaacgattc ctaactcgcg tagttctggc gcagtaattt tcatcaactc agctgcttct
    16021 gcggcgcgac taccgtcttt ccacaaaatc gacgcaaatc cttctggaga aagcacagca
    16081 taaatcgttt cttccagcat ccagacttca tccgctaaac ctagagcaag ggcaccgcca
    16141 cttccacctt caccaatgat gatggcgatg atcggtacct ttaaatcaga catttcgagc
    16201 agatttctgg caatagcctc tccttcgccg cgctcttctg ctcctacacc gcaatatgca
    16261 cctgctgtat tgatcaaggt aatcaccggt ctattgaact tttccgcttg tttcatcagt
    16321 cgtaaagctt ttcgatagcc ttcaggatgg ggagaaccaa aatttctgcg gatattttcc
    16381 tgcagatccc gtcctttttg aataccgatc actgttaccg gtttgccatc taatcgagcg
    16441 attccaccaa caatcgcttc gtcatctgca aataaacgat caccatggca ttcctcgaaa
    16501 gaatcgaaga tctcttcaaa aaaatccaag cttgtccatc gttctttagc tcttgctaac
    16561 tgaacgacat catgtgctgt cttatccacg atctatccat cctttctgtg tatgcaattc
    16621 aagaagcgta tggattttct gtttcaattc catgcgggga acaatctgat cgacaaaacc
    16681 atgagataat aagaattcag ccttttggaa atcttctgga agttcctgct tgatcgtctg
    16741 ttcgatgacg cgtcttccag caaaaccgat caagctttgg ggttctgcta gaataatatc
    16801 gccttccatt gcaaaactgg cagttactcc tccggtcgtc gggtccgtta aaacagttag
    16861 ataaaaaaga ccttcattgc tatgccgttt gacagcagca gagatttttg ccatctgcat
    16921 tagagaaaag attccttctt gcatccgtgc gccacctgaa gctgtgaaca agacgactgg
    16981 aagactttcc tttgttgctc gttcgaataa tctggtgatc ttttctccga caatcgttcc
    17041 catacttccc atgataaaat taggatccat cactccaata atgaagggaa tcccctgaat
    17101 ggttgcttta ccagtgagga ctgcttcatg aagtcctgtt ttatcttgca tttttttaat
    17161 tttttccgta tattcgggaa atgctagcgg atctttggtt acaagatccg tatcccattc
    17221 ttcaaaactt ttttcatcaa taatcaaagc tagacgctgc catgcaccga ttcgaaaagc
    17281 gtatccgcaa tgagggcata ctttttcttc accgatgtct ttcgtataaa ggatatgttt
    17341 gcaatttgga cactttgccc acatgttatc aggaacagat ggcgagttag caggtttgct
    17401 ctctctatta ggattgatcc gaatatattt ctttttctta aacagagcca tctatgcgcc
    17461 tcctatctcc gtttctggtg tccaattcgg taaaaattct tcttgtaaaa atgctgtgct
    17521 gtaatcacct gctatgactg caggatgact aatcaaatcc atctgaaatt ctgcgttcgt
    17581 gatgattcct tctgtgataa attcacttaa ggctcgctgc atcttcatta atgcttcaaa
    17641 acgcgtattt ccatggacga ttatttttgc aatcattgaa tcataatatg gcgggatcgt
    17701 atatcccggg taaactgcac tgtctactcg cagtcccatc ccacctgctg gaagcagtaa
    17761 gttttggatc ttccctggag aaggagcaaa atggaaagca ggattttctg cattaatccg
    17821 gcattcgatt gcgtgtcctt ggaatactac ttcttcttga cacacattca aaggctcacc
    17881 ggctgctatc tccacctgtt tttttactaa atcgattcct gtgaccattt ctgttactgg
    17941 atgttccact tggattcgag tattcatttc catgaaataa aaatcgcctt cttcatccat
    18001 cagaaactcg atcgttccgg cattttcata attgactgct ttagctgctc gaacagcagc
    18061 gtctcctagt gcttctcttt ttgtttgtga gatcactaca gaaggagatt cttccagtac
    18121 tttttgattg ttgcgttgta aagaacaatc tcgctcgcct aaatgaatga catgaccaaa
    18181 tcgatcacct aagatctgaa cttctatatg gcgagcagga taaatgattt tttccaaata
    18241 catatcatca tttccaaatg ccgctgctgc ttcttgctga gctgaagaaa aatgctgtgg
    18301 gagctcttct tttgattgaa ctttccgaat gcctttcccg ccaccacctg cagctgcttt
    18361 cagcatcact ggataaccaa ttctgtctgc tatttccaat gcttcttcta ctgtagataa
    18421 tacaccagta cttcctggaa tgactgggac atttgcttct tgcatcaatt gacgcgcatt
    18481 gatcttattt cccattgcat cgatcgttgc acttttagga ccaataaatg tgatattgca
    18541 ctcttcacac atggaggcaa atcggctatt ttcagataaa aaaccaaatc ctggatgaat
    18601 tgcttcggct tttgtcacga ttgcagcgct cagtacttcc tgaacattca gatatgaatc
    18661 aacggctttt gccgggccaa tgcagattgc ttcatctgct aactgtgtat gaagtgcttc
    18721 ggcatctgct tctgaatata tcgctacggt ttggatccct agttcgcggc atgcacgaat
    18781 gatacgtaca gcgatttcac cgcggttagc tatcaatact ttagaaaaca tatttcacct
    18841 atccaatcat aaaggttaat tccgcttcgg ctactttttt accgtctact ttagcgattc
    18901 ctttgcctat tccggctgaa gctttcactt tcaatatctc aacttcaagg tataaggtat
    18961 ctcccggtgt aactttctta cggaattttg ctttgtccaa tccgccgaaa taagccgtct
    19021 ttcccttgaa ttgttcaaga gaaagtaatg caactgctcc tgcttgagcc attgcttcaa
    19081 caatcaatac ccctggcata acaggttctt ctggaaaatg tccttgaaaa aagggctcat
    19141 tgatcgttac atttttctta gcaacgatcc gttctccttc aaccatttct tccactcgat
    19201 caatcaaaag cattggataa cgatgtggaa tgatttcttt tatttcttgt atgttcattg
    19261 ttttccctct ttcctgcttt gcttattttt gctgaatcct aaagagtggc tgtccatatt
    19321 cgacaactga ctcgttttct acaaggattt ctgtgatcac tccgtcggcc tcactagtta
    19381 tctcattcat cagtttcatc gcttcaataa tgcagataac ttctcctttg gcaacttgat
    19441 cacctacttt tttgaaagct ggtttgtcgg gcgctggttg cagataaacg attcccacga
    19501 ttggcgaaac aacctcgatt ccatcaaata ctgccgattc tttttctttc attatttctg
    19561 gagcagttgt tgcttgttca gatatgacat tgactggctc cgaaactggc aacgtttcgt
    19621 ttgtttgaac catattcatg cctcgacttg ttttattttt attcatatac aattcaaatt
    19681 gaccttcacg caaatcgaat tccgttagcg aggattgatc aaattgactg actaattctc
    19741 tgatttcatt gatatccaaa attattcctc ccagcgtttc atgcacagaa ccgcattatg
    19801 tccgccaaac cctaatgaat tactcattgc ataagtaaaa cttgcaggtt tgctttcgtt
    19861 tttgacaact gtcaagtcaa tctcaggatc ttgtttttct acattgatcg taggtgggat
    19921 aaattgatgt tctaatgcca gcaaagttgc caaggcttcg atcccgccgg ctgctcccaa
    19981 taaatgtcca gtcatcgatt tcgtgctgct gacgtaagta tctctgaaat tttcacctaa
    20041 tgcgatttgg attgcttttg cttctgcctg gtcattggct ggtgtacttg ttccgtgagc
    20101 attgatatat ccgacttgtt ccggctgtac accagcttct tccatcgcaa gcttcattgc
    20161 tttagatgca ccagaaccat ctggagtagg agctgtcata tggtaagcgt cacagtttgc
    20221 accgtatcca acgatttctc catagatttt ggcaccgcga gcttgtgcat gttctaaaga
    20281 ttcaagaact agaacgcctg ctccttcacc catcacaaaa ccattccgat tttcgtcaaa
    20341 cggaatcgaa ccacgattag gatcagttga ttctgagagt gctgttaaag ctgcaaatcc
    20401 agcaatcccc atctcacaaa tcgttgcttc tgttcctcca gcaagaatga catcagaata
    20461 gccatgtttg atgttacgga atgcttcccc aattgaattc gttccgctag cacaagctgt
    20521 tacgatagct gtacaaatac cttttgcacc tacacgcaaa gcaatatttc cagctgccat
    20581 gttggcaatc gccattggta cgaacattgg agaaactcgt tttgcacctt tttcatgtag
    20641 acgaataact tggttttcga ttgttggaag accaccgatc ccagatccga tgatgacgcc
    20701 aaaccggtct tgattcattt tttctttatc taaaccgctc atctccattg cttctaaagc
    20761 agcgtagatt ccatagatcg aaaacatatc cattcgtttg ctatcttttt taataaaata
    20821 tttatcgtaa gggaaatctt tgacttcacc cgctaaggta atccctgttt ctgaagcatc
    20881 aaatttagtg atcggtgcga tgccattcgt cccctctttt aagctattca aaaagctgtc
    20941 tgcgtcattt ccaataggtg aagtgactcc atagccagta attaccactc gattcatttg
    21001 tttgctctcc ttttcttatc cgtgcatgac caatccgcca tcgacattga tgacttgtcc
    21061 ggtgatatat ggattttgtg ctaagaaaac tgctgtttgt gcaatatctt ctacttgtcc
    21121 gaaacgctgc atcggaattt gcttttccgc ttgttccttg actttgtctg ctaaaacttc
    21181 cgtcatatca gttgtgatga atcctggcgc aatggcatta catgtaatgc ctctagttgc
    21241 agcttctcgt gccacagatt ttgttaatcc tactacgcca gctttactag ctgcataatt
    21301 tgcttgacca atatttccaa tcaacccaga tacactagag agattaatga tggccccttc
    21361 tcgctgcttc atcatttttt tcaatacgtg ctgcgtcata ttgaaggttc caatcaaatt
    21421 gatatccaga cattttttga aatcttctgc atccattcgc atcacaagtt tgtcatttgt
    21481 gattccggca ttgtttacta acacatggat cgatccaagc ttttcttctg cttctttgat
    21541 catttgtccc gctttctcat aatcagagat gtcaccggaa actcctacgc attttacacc
    21601 gaatgcttcg atttcttcta tcttttcttt tggaatctct ccgcgaccat tcaaaatgat
    21661 atttgctcca gcttttgcaa aagctaaggc catcgctttg ccgattcctc ttgtactacc
    21721 tgtgataaat acattttttc cttttacatc cataacgttc ctccttatag tattgctatt
    21781 gtttcatcaa atgtctgctt attctcaaca cgcagaactg gtattgtttt atcgattttt
    21841 ttcataaatc cgcttaatac ttttccgggg ccgacttcga tgacttgttc gatgccgatt
    21901 gtttttaact tatggatact ctcatagaaa cgaacaggtt gcatgacttg ctgttcaagt
    21961 agtcccgcga ttgtttcttt cttcattatt tccgttgttg tgttgcttat gataggaaaa
    22021 gaaggttcag agaattgaat ctgacttagg tccttcgcca gtttttttgc tgccggttct
    22081 aaaatagctg tatgaaaagg cccgctgaca tttagaggga tcattctttt gaaccctttt
    22141 tcttttaata gtaaaacagc ttcgtctact gctttttctt cgccaccaat cacgatctgc
    22201 tgaggtgtat tataatttgc aggagatacg atgccgtact tgctagcttc atgacagctt
    22261 tcctcgatcg tttcaatcgg cgcattcatc actgcaacca ttttcccgct tccagctggg
    22321 gtagcttctg tcatataagc gcctcttttt gctactaacg caactgcttc agtgaaggat
    22381 agcgcattac tagctactaa tgccgaatat tctcctaaac tcagtcctaa agctgcatct
    22441 gggatgattc catgctcctt caataagcga taatacgcga tgctgactgt tagaatagct
    22501 ggctgtgtat attgcgtctg atccaaacgt tcattttctg taaagcaaag ttcagccata
    22561 tcgtagccga gaatctcact tgcttcatca aatgtctgct ttacaacggc ttcttcatat
    22621 aaatcttttc ccataccttg atattgtgca ccttggccac taaataagaa tgctgttttc
    22681 atgtttttct ccaatatatc cagaatatat gtttgattct tcctattcaa aaaggccgag
    22741 acattcctat ttcaggaaat gtcccagccc ccaacctaat tttaatgatt atgcagtttg
    22801 tttttctacg taagtcacta aatcttgaac cgttttgatt ccgtcttctg tttcgatttt
    22861 cacgtcaaat tcatcttcaa tttcattgat gatttggaat aaatccaagc tgtcagcttc
    22921 taagtcttct tggatgtcag tagtcaattt gatttcttca gtttcttttc ctaattcttc
    22981 tgcgatgatt tcttgaattt tttcgaatac cataataata tattcctccg atattgtttg
    23041 ctttttattt ttataattta acagtctaca actccatgag aatacttccc catgttaaac
    23101 ctccgccata gcctgtaaat attactttct gctgttttcc taaggtgatc tttccactag
    23161 aaactgcttc atccaataag atagggatgc ttgcagcaga tgtattccca tatttatcca
    23221 tattggtcag gaacttttct tgcggcatct ttacttttcg ggcaattttc tcaataattc
    23281 tgaggtttgc ctgatgcaaa agcagataat caactggttt gtctttcatg acttcttgga
    23341 tattgtttgc aacgtctcgt acggcaaaat caaaaatatc tctgccagtc atttgcaaat
    23401 aatacgaacc ttctgaatct tctgaataga acggattttt attttgctgg tagccagcgg
    23461 tcaatgagct acttctggta ccgtcagctg ctaatttttc tttcagcaat tttttctgtg
    23521 gattcgcttc taagagaatc ccacctgctc catcgccaaa caacacagca gtcgtacgat
    23581 cagaccaatc gattagctta gatagtgttt caccaccaat caccagtccc ttagttgagc
    23641 cgcagcggat cattttttca gccatgctta acgcataaac aaatcctgag cacgcagcac
    23701 tgatatcaaa agcgatagca tgagttgctc ccaatttccc ttgtacttgt gctgctacag
    23761 atggtgtcgt gtaatcgggc gacattgttg ctactaaaat aaaatccaaa tctttagggt
    23821 ttagattttt ctgttttatc aatttttccg ccactaaaat gcataaatca gaagtctcct
    23881 gatttgttac acatcttcgt tcacgaatac ccgtccttga cgaaatccat tcatcagagg
    23941 tatccatcat cttggaaaga tcattattag taataatctt ttcaggaagg acgctggctg
    24001 ttgcagtaat cattccaaat tgttccatta aatatcctca cttatagtct tgcaagaagt
    24061 cgtgcaagtt tttcagtgca tccagcaagg catgctgttc ctccgtagcc attccgtcta
    24121 agatgttctt gaccatttca cgatgaaaat gctgatggac ccgaaatagt aactttcctt
    24181 ttttcgttag tcccaacttt acaacgcggc ggtcatcttc actgcggata cgttcgacat
    24241 agcctttttt tactaacttg ttaatagcag tggtcaaggt cccaacagta attgatagtt
    24301 ctttggctac ttctgaagtt gtttttttct tgtacatccc gattgcctcg atcgtgtgca
    24361 tctctgtgat tgacagatct ttgaattgag attttttcag ctcagactct tcgattgtca
    24421 aaatatcatt gaagacacta acaagataat catttactgt ttttaaatct ggttccatgt
    24481 tccaccgtcc actaattagt ttgattatca aatcttttga tgttcaaact atatcttaac
    24541 ttttttcaaa atgcaacctt aaaagatcat ttttttacta tatgagcttt ttttaagatt
    24601 ttactcattt aaaacaactg ataaatcagc atttataatt tcttttatta gaaaaatcat
    24661 ctatctcaca tattttgatt atcaaagtat tttctgtgaa cagaactata gaattcccta
    24721 aaacttgtcc attatttcaa aatttgttat agtcttttta ttcaaagcgt aaaaggagat
    24781 cctatgttaa aagaaaaaat gaaaactctc cccatgctgc cgggtgtcta tcttatgaaa
    24841 gaccgagatg gtaaggtcat ctatgttggt aaagcaaaac ggctcaaaaa tagagtaagc
    24901 agctattttc atcagaataa acagcattca aaaaaagtat tacgtatgat tcatcatata
    24961 acagattttg atttcgtcgt agtagacacg gaattagatg ctttattgct cgagtgtcaa
    25021 ttgatccaac attaccgtcc tttttataac cgacaaatga attatttcag taattataat
    25081 tatgtacata ttaccaataa gggatttgta ttaacagata ctcctacggc tcgaacatac
    25141 gggccttttc gtctctataa aaaaatgccg tctattttgc gtataatgga agaaacttat
    25201 caaatgcctt ggttgtctga aattagtttg ttagctttgc gagtccagct tcctgaccta
    25261 caggaaatga cttttgaaca gaaaaaaaag gaactccaag ggctgtttca aggccgaaat
    25321 aaaaagctac tgacttactt gaaaaaaaga caacaacatt tcatctatca gttgaatttc
    25381 gaaaaagccg gtatgcttca aaaagatatc gaattagtta cttattttat tagacggatc
    25441 caggaacaaa agcagttttt aagaacacca tcgctcacct tcagcatgcc attagcagca
    25501 gatgagagtc agaaaaagca ctacctcgtt tgttatggtc aacttgcaga aacaatgatt
    25561 acctctggtg acgtttctcc tgacttctat tatgaaaaga aagaagctca tttatctttg
    25621 aaacgtcaat tgtcaaaaga agaaatcgat cctgtacaaa tcttgatcag ttaccgcaaa
    25681 aaattagaaa aagaacagtt tgaaattgaa ctgttaaaca aaaaagaagc tgaaaagcaa
    25741 ctaaattagt tgcttctcgg cttcttttag gcatcttcaa cttcaaatgt caattatcct
    25801 aaaatttatt ggattaagtc atagatttcc atcgctacga tatcgatatt atcaaattcg
    25861 tattgctgat gagtatctgt ttcagttaat tcaaatgtat cggtcgcttt atcataagtt
    25921 actgtacact tctctacgcc ttctttttca aaacggcgaa cttgcagttc attatctgct
    25981 gactcgacca tagcctccag tcgttttatg attgcgacta attgtgaatg tgtcatcgga
    26041 ggtcctcctt ttcttatttc tgttatattg tatcaaaagc atagcctact tgcattgatt
    26101 ttctctaaaa aaactcgttt tttatgattt tttttctggt atttttcctt caggccacca
    26161 aagtttgatc aaagcttggg ttccgtcatt ctcaaaacct ttatctgcca actcttcata
    26221 tagtttcaat gcttgggtag ttgctggcaa tacaagatcc attttttctg cttcttctaa
    26281 tgcaatcttt aaatctttga taaaatgttt gacaaaaaaa ccaggtgtgt aatcttcttt
    26341 taaaatacga ggagaataat tgcttaaaga ccagttagct gcacttccac ctgaaagcgt
    26401 ctcaatcact ttctttaaat caagtccgtt tttttgtgca tagacaagca tttccgtcat
    26461 tcctgtcatc gtacctgcaa tcatgatctg attagccatt tttgtatgct gacctttccc
    26521 agcagaacca tgcaatgtaa atgtcgtacc aaattctttg aataaaggca gtacttgatc
    26581 ataaacggct tcttctccac cgaccatgat cgtcagtgta ccattctttg ctcctaagtc
    26641 accaccggat accggtgcat ctaacgcatc tgcgcctttt tcttttgctg tttttgcaat
    26701 tttttctgcc aggcttggtg tacttgtagt caaatcaact aaaatatgtc ctgagatatc
    26761 tgcagaaaaa atccccgact gtccatagta gatttcttct acatctttgg ggtaacctac
    26821 cattgtaaag agaatattac tttgttctgc taccgctttt ggtgtatctg cccagatagc
    26881 tccttctgtt actaaaggat cagcttttgc ttttgtccga ttatagacag ctacttcgtg
    26941 tcctgcttct agtagatggc gtgcgacagc acttcccatg acgcctgttc cgataaaacc
    27001 aagtttcatt ttttcgacct cctaaaaatc aaaaatcctt gcttctatgc tagtatacgc
    27061 tagaaacaag gattttttca taaaaaatgt ttgaatttta tcttttaaat gaatttaatt
    27121 ttattttgat tcgttcaatc gagtcttctt cttcttttct tcgcatctct tcttcatgat
    27181 aaacaaccat gtcttgataa aattcgatca tagcttcgcc gaaatgtgta gatgaaatat
    27241 cataaagttt agcttctaaa acttttggat ctttaggaac ttcactggaa atataatcaa
    27301 taagtgctgg tgcaaagtct tcgtcggttt caaaagtttt ccctaatgaa ggatgatcga
    27361 acaatcggtt caaataagca tttccttcca cgacacaagg tgttccggct gccatcgctt
    27421 ctgtataagt aagcccttgt gtttctgaag tagaagcact gacaaaataa tctgccgctt
    27481 tataataaag tgctacatca ttgttgtcca cttctcctgt gaactgcaca aattcttcta
    27541 actgcagttc tgagatcaat tcctctaact cttctacata aggtcctttt cctacgataa
    27601 ctaatctggc gttaggataa gcggagatga cctgaggaaa cccattaatc aaggcttgga
    27661 tatttttctc ataagaaatc ctgcttaaag aaagaatcat caaatggttt tcctgaagac
    27721 ctagtgactc ccttaatttg ctgatatcct gctgagtgat atcagaacgt ttgaattttt
    27781 caatgtcgat accagtagga atcacgcgaa gcggtgctat tacaccatag ctgcgtaatg
    27841 tatcaataac acgttcactt ggacaaacta cacctgtcgt atgatttgca aataatctag
    27901 acaagtattt tacatgggat tgcctgatca cttttccttt accgatataa tgaaggtagt
    27961 cttcatacat cgtatggtaa gtatggatca gaggtatttt caacttctta gctaccatct
    28021 tcccaagtat ccctgctccg aattctgtat gggtatgaat aagatctagt tctagttctt
    28081 tggcaaccaa ataagcatac cacatgcctc taacaacaac tcttcgatcc ttgaaagaaa
    28141 taaatggtac acttggcatt cgaatcacat ctttttccaa cttatccgca ttaggatcgg
    28201 tcgttgtaaa aatatagact tcatgcccat gtctttctaa ttcttgtttt aatgttttga
    28261 tcgatgtagc aactccgctg acttgcggaa agtaggtatc ggtaaaaaag ccgattttca
    28321 tgtttttcgc ctccaaacta gtccttgacc tgatttactt tttttaattt gctagagact
    28381 ttttcattca gtacactttc gtatacattt ttaagttctt caccgatttt attgatactt
    28441 cgctcttttg ctgtttgata accggcttgt gtcgtactcg gtagttcttg attggtaatc
    28501 tgttggatca gtcggataaa ttcttcgttt gtcttcccca tatagcagtt cttgctatct
    28561 tctaaccagc cattatacac tggaatatct cgaacaagta cgttttgttt gcttgctaaa
    28621 gcttctaaaa caacgatccc ttctgtctct tcataagaag ggaaaaagaa tagatctgca
    28681 cctgaatacg caccttctat gatctctcca cgaatgtaac caggaaacag gacattttca
    28741 ggatgatctt cttttacaat tttccggatg ttccgaggaa tcgagtacat gggaacatga
    28801 ccaaaccaaa taaatgtgta ttcgggcatc ttcttagcaa tttctacgaa atcaatgatt
    28861 cctttacgtt cgaaaaacaa accgacacaa atgattactt tttgatccgg agacagtttg
    28921 aaatattctc tgaacttttg ttctttcatt ggatcagggt gatatttctc taagtcgatc
    28981 ccattggaaa tagcttgaat cggcacacgg ataccatagc tttccaacaa ctgttttgaa
    29041 taaggtgttg gcgtgatcag atagtccgct ttttggtaca atcctaccag atattttttt
    29101 acgataggag aaagctggtt cgatccgata aaggaatttc ggaaatcttc ctctgtagaa
    29161 tgcgcatgat agacaacttt ttttccttct cttctggctt tattgaccat attatgacta
    29221 ttgattccgt atgtattgat gtgcaggatg tcataatctt ctttttcatc tagtgtataa
    29281 gagataccga cttccgtcag agcacgtttt tgatgatcaa gcgcacgccc gattccagat
    29341 tttgcaagca tcttttcact ttcaaaatat aataatacct tcaccttacg tcccccttca
    29401 cttatatagt ataacatagc ctacaaactg tcactcaaac ttaagcccta tttttgtcta
    29461 ttcttactga caaaaacgtt ttcttaaaaa agaacaaaaa aactggcgta gctcttttac
    29521 aagccacacc agcttttgat acgttgttta ttttacatat tcatgaacaa gttcaacgac
    29581 ttcttccatc atatcgcatt ctttcaatgc acgatcagca agttcagcca ttttcgctgt
    29641 gtccaagcgt ttcattaaac tacgcgtttt aagaatagat gtcgcactca ttgagaactc
    29701 atctagaccc atacctacta gcaatggaac agctgtttga tctccagcca tttctccaca
    29761 catacctgcc cattttcctt cagcgtgtgc tgcatcaatt acatttttga tcaaacgtaa
    29821 aattgatggg ttatatggtt ggtacaagta agaaacgcgt tcgttcatac ggtctgctgc
    29881 cattgtgtat tgaatcaaat cgttcgtacc tacagagaag aaatctactt cttttgcaaa
    29941 cttgtctgct aagactgctg ctgctggaat ctcgatcatg atgccgactt ggatcgtatc
    30001 agatacttcc ttgccttcac ttatcaattt ttgtttttct tcttcaaaga tcgcttttgc
    30061 tgcgcggaat tctttgagtg tagctaccat agggaacatg atacgtaaat taccatgaac
    30121 agatgcacga agcaatgcac gcatttgtgt acggaacatt tcgtcgcctt gttcagataa
    30181 actgatacgc aatgcacgat atcctaagaa tggattcatt tcatgaggta attgcagata
    30241 tggtagttct ttatctccac cgatgtccat tgtacgaaca actacaggtt ttccttccat
    30301 accttctaat acagctttgt aagcttcata ttggtcatct tctgttggga agtctggaga
    30361 atccatatac aggaattcag tacggtacag accaactgct tctccaccat tattatggac
    30421 accaactaga tcttttggtg ttccgatgtt tgcagctaat tcaaaatgct tgccgtcagc
    30481 tgtgacagtt tctgcatttt ttaatttttc ccattctgct ttttgtgctg cgtaatctgc
    30541 acctgctttt tcaaattcag ctttttgttc atctgtagga tcgatgatta catcgccttc
    30601 gatcccgttg acagccaaaa tatcgccttc tttgacttta gcagtaattt cttttgtccc
    30661 aacgattgct ggaatttcta aagaacgtgc catgatagca gaatgtgaag tacgtccacc
    30721 aatgtctgtt acaaacgctt ttacaaaatt gcgatctaat tgtgctgtat cacttggagt
    30781 caagtcatgt gcaacaacga ccacttcttc gttgatcatt gatggatttg gtaaagtaac
    30841 gcctaataag tgcgctaaaa tacgtttagc aacgtcacga atatctgcag cacgctcttg
    30901 catataagca ttatcttcca ttgcttcgaa cataccgata tacatatccg ttacttcttt
    30961 tagcgctgat tcagcattta cgctgttgtc tttaatattt tgcttgattt gacctaccat
    31021 ttctggatct gaaagaacca tcaaatgggc atcaaatact tgcgcttctg cttcacctaa
    31081 gctttgcgct gctttttcac gaatttgctg caattcttca gtagatttag ctaatgcagc
    31141 atccaaacga gcttcttcag ccgcagtatc ttctactgtt gccttactga atgttaaatc
    31201 cggttgaact agcaggtaag cttttgcaac ggctacaccg tcactagcgg caatcccttt
    31261 tagcatttca accattattc agataagcct tctttcttca ttgtttcaac gattgctgcc
    31321 attgcatcag cttcgtcagc accttctgca gtaatagtaa catcagaacc ttgacctaca
    31381 cctaaagaca taacgcccat gattgatttc aagtttactg atttaccttt atactctaag
    31441 tttacatcag agttaaattt gcttgctgtt tgtactaata gtgtagctgg acgagcgtgg
    31501 atcccagttt ctgctactac gtgaaattct ttcttttcca tattaatcgg tctcctttaa
    31561 gtgagtgttg tttttatgtg agggttttct tttttagtaa ttcagagaat cctaacaaat
    31621 gccacccttt catctgtaaa gattaacatt ttttttcgtt agatacaaca gttttcattc
    31681 aatttcagat aaaagtattc aaattattcc ggattctttt tcatcctttt cttcacattt
    31741 tataacaaac gtgtttattt cctttttctt gtcgctttct cttctcctga cgtttatcat
    31801 tctttccttt gaccctcttt gttttcatat gaaacgaatt acttgcttcc ctttttattt
    31861 ttagtataat aagagtcaaa ggtcaggaat ggtcaataga gatttcacct tttctacctc
    31921 ataaatctct taaaaaacta ggggttattc ttttacctca tgaaaaacga gatgtaaaat
    31981 atggatgtac ccatttttgt gaaaggacgt gaatgattca tgctttgtca aaactgcggc
    32041 aagaatgagg caacgattca cttgtatgcc actgtcaatg gtcagcggac acaactcgat
    32101 tactgtcaga gctgttatca aaaaatcaaa aatcaacaaa atggaggtct gactaatatg
    32161 gcccaaaatg atccatttgg atttggcagt ttggacgatc ttttccgttc catgtctcgc
    32221 caaatgcaac agcaaggaaa ttatgagcaa acaccaccaa cacaatttgg cggaaacaac
    32281 aacttcaatg gtggtcagcc acctcaaggc aatacagaag gtttattggg agaatacgga
    32341 atcaatatca cagaacaagc acgacaaggt gatatcgatc ctgtgatcgg acgcgatgac
    32401 gaaatcaaac gagtcattga aattttaaat cgccgaacca agaacaatcc tgtcttgatt
    32461 ggagaaccag gtgttggtaa aacagcagtt gttgaaggtt tagcacaaaa aatcgtagat
    32521 ggtgatgtac cgcaaaaatt aatggacaaa gaagtcattc gtttagatgt cgtatcttta
    32581 gtccaaggta ctggtatacg aggacaattt gaagaacgta tgcaaaaatt gattgaagag
    32641 atccgtcaag cagaaaatgt gatcctattc atagatgaag ttcacgaaat cgtcggtgct
    32701 ggttctgctg gtgatggaaa tatggatgct ggtaatattt taaaaccagc tttagctcga
    32761 ggagaattgc aaatggttgg tgctactacc ttaaatgagt accgcatcat cgaaaaagac
    32821 gctgctttag aacgtcgtat gcaacctgta cgggtagatg aaccaactgt cgaagaaacg
    32881 atcagtattt tgaaaggttt gcaaaaacgt tatgaagact atcatcatgt aaaatatact
    32941 gatgaggcca tcgaagcagc cgcaacttta tccaatcgtt acgtccaaga tcgcttcttg
    33001 ccggataaag ccatcgattt gctagatgaa acaggttcta agaaaaactt gacgatccaa
    33061 atcgttgatc ctaaaacaat cgagaaaaaa ctgcaagaag cagaagaaca aaaagtttta
    33121 gcttctcgag aagaagattt cgaaaaagca gcttattatc gcgatcagat caataaactt
    33181 caaaaaatga aagaacgtca gctcactgaa gaggaaacac ctgtgattac cgaaaaagat
    33241 atggaaaaaa tcgttgaaca acggacaggt attccagtgg gtgaattaaa agaaaaagaa
    33301 cagacacaac tgaaaaactt agcagatgac ttgaaagctc atgtcatcgg acaagataac
    33361 gctgtagatc gagtagcgaa agcgattcgc cgaaaccgtg ttggattaaa caaacagaat
    33421 cgtccaatcg gctccttctt atttgttgga ccaactggtg taggtaaaac agagcttgcg
    33481 aaacaacttg cctacgaact attcggttca caagattcaa tgattcgttt cgatatgtct
    33541 gaatatatgg aaaaacatag tgtctctaaa ttgattggtt ctcctccagg ttatgtcgga
    33601 tacgaagaag ctggtcaatt aacagaaaaa gtacggagaa atccttacag tcttgtcttg
    33661 ttagatgaag tagaaaaagc tcatccagat gttttgcata tgttcttgca aatcttagat
    33721 gatggtcgat tgacagatgc acaaggaaga acggtcagct ttaaagatac gatcatcatt
    33781 atgacaagta atgccggaac tggtaaagta gaagcaaacg ttggttttgg tgcggcacgc
    33841 gaaggtgtta ctcgttctgt attgaaccaa ttaaacaact actttacacc agaatttctg
    33901 aaccgttttg acgggatcat cgaattcagt gcattatcaa aagaaaactt gatgacaatc
    33961 gtcacactaa tgctagacga tgtcaaccaa atgctggcag cacagcaact tcacatcgaa
    34021 gttcctacga atgtcaaaga aaaattagtt gacttaggct atgatccatc tatgggtgct
    34081 cgtcctcttc gacgcacgat ccaagaacaa atcgaagatg gtatcgctga attttatctt
    34141 gatcaccctt ctatccatga gttgaaagca aaactggata aagatggaaa aatcatcgtt
    34201 acttctaaac cagaacgttt ggctaaagaa tcagccgaag aaacggctga ataaatccag
    34261 ttttatagcg tgagacggta tttctttgag aaatgccgtc tcattctttt ttcagaaaag
    34321 tcaaaatact gttcatttta taaaaaagaa attataataa gagaaacaag caattaaaaa
    34381 tacataaaag aggtgagcgc catgaaattg gtaaatgtaa caaacagcca ttcccgtctt
    34441 gtattaaacc agcttgaaaa cactgatgcc catctagtca aggtctacac agccggaaat
    34501 accactattg tctacactga agcccctgaa cataatgaaa ttctgctgat caatgacaaa
    34561 cgtaaaatcc agccgaaaga aatcgaagat gctaaaaatt atttcttaga taaaatcaaa
    34621 gacgccgttt accacgagga tgaaatcaaa gtaatcgaat tggcaggcct tgttgaaatc
    34681 tcgattccaa aacatgaatt aggcgctaca ttagccgtgt aaacaaattt tgaacataat
    34741 aattaaagag cgtgggacaa aagtaaagaa cacttttgtc tcacgctcta aacacgtata
    34801 aacggcgaaa gcagaagcaa ctccttcgga aataagctga aattcacgaa aattgctttt
    34861 caaatttttg tgaattccag cttatttctt ggagttaaac gcttctgtct cagcctcttt
    34921 ttctttctag ctttttgatt tttctctttt cttttgtact tttctctttt cattttcagt
    34981 tctacgcgtg tctttatttt taactttttc ctctttatgg atgattgatc gtactttttg
    35041 tgcaatttgc tcgtttttgt cccagtcttt tacatctaat aatcgacata atcgtaacat
    35101 ctcttctttt ttctgttttt ttctttcacg tctttgccat tctgtttcca tgtgtcacac
    35161 tctcctttct ttaatcatat gtacgcaaaa taatttttat atttttttga ttagac
//
LOCUS       DAZABD010000025        34909 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000025 DAZABD010000000
VERSION     DAZABD010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34909
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.025"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..96)
                     /locus_tag="ACHALG_001279"
     rRNA            complement(<1..96)
                     /locus_tag="ACHALG_001279"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            837..1526
                     /locus_tag="ACHALG_001280"
     CDS             837..1526
                     /locus_tag="ACHALG_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFN2586702.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1513..2721
                     /locus_tag="ACHALG_001281"
     CDS             1513..2721
                     /locus_tag="ACHALG_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFN2586703.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            3015..4286
                     /gene="serS"
                     /locus_tag="ACHALG_001282"
     CDS             3015..4286
                     /gene="serS"
                     /locus_tag="ACHALG_001282"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFN2586704.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4835..6319)
                     /gene="guaB"
                     /locus_tag="ACHALG_001283"
     CDS             complement(4835..6319)
                     /gene="guaB"
                     /locus_tag="ACHALG_001283"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFN2586705.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6824..7519)
                     /locus_tag="ACHALG_001284"
     CDS             complement(6824..7519)
                     /locus_tag="ACHALG_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HFN2586706.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7609..8709)
                     /gene="ychF"
                     /locus_tag="ACHALG_001285"
     CDS             complement(7609..8709)
                     /gene="ychF"
                     /locus_tag="ACHALG_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFN2586707.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8718..8909)
                     /locus_tag="ACHALG_001286"
     CDS             complement(8718..8909)
                     /locus_tag="ACHALG_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HFN2586708.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8930..9820)
                     /locus_tag="ACHALG_001287"
     CDS             complement(8930..9820)
                     /locus_tag="ACHALG_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFN2586709.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9807..10574)
                     /locus_tag="ACHALG_001288"
     CDS             complement(9807..10574)
                     /locus_tag="ACHALG_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFN2586710.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10586..11299)
                     /gene="rsmG"
                     /locus_tag="ACHALG_001289"
     CDS             complement(10586..11299)
                     /gene="rsmG"
                     /locus_tag="ACHALG_001289"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFN2586711.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11531..13432)
                     /gene="mnmG"
                     /locus_tag="ACHALG_001290"
     CDS             complement(11531..13432)
                     /gene="mnmG"
                     /locus_tag="ACHALG_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFN2586712.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13449..14846)
                     /gene="mnmE"
                     /locus_tag="ACHALG_001291"
     CDS             complement(13449..14846)
                     /gene="mnmE"
                     /locus_tag="ACHALG_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFN2586713.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            15145..15528
                     /locus_tag="ACHALG_001292"
     CDS             15145..15528
                     /locus_tag="ACHALG_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2586714.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15696..16454)
                     /gene="jag"
                     /locus_tag="ACHALG_001293"
     CDS             complement(15696..16454)
                     /gene="jag"
                     /locus_tag="ACHALG_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HFN2586715.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16472..17290)
                     /locus_tag="ACHALG_001294"
     CDS             complement(16472..17290)
                     /locus_tag="ACHALG_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HFN2586716.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17287..17643)
                     /gene="rnpA"
                     /locus_tag="ACHALG_001295"
     CDS             complement(17287..17643)
                     /gene="rnpA"
                     /locus_tag="ACHALG_001295"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFN2586717.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17761..17895)
                     /gene="rpmH"
                     /locus_tag="ACHALG_001296"
     CDS             complement(17761..17895)
                     /gene="rpmH"
                     /locus_tag="ACHALG_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFN2586718.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18418..19752
                     /gene="dnaA"
                     /locus_tag="ACHALG_001297"
     CDS             18418..19752
                     /gene="dnaA"
                     /locus_tag="ACHALG_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFN2586719.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19950..21080
                     /gene="dnaN"
                     /locus_tag="ACHALG_001298"
     CDS             19950..21080
                     /gene="dnaN"
                     /locus_tag="ACHALG_001298"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFN2586720.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            21309..21551
                     /gene="yaaA"
                     /locus_tag="ACHALG_001299"
     CDS             21309..21551
                     /gene="yaaA"
                     /locus_tag="ACHALG_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HFN2586721.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21538..22662
                     /gene="recF"
                     /locus_tag="ACHALG_001300"
     CDS             21538..22662
                     /gene="recF"
                     /locus_tag="ACHALG_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFN2586722.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22659..24605
                     /gene="gyrB"
                     /locus_tag="ACHALG_001301"
     CDS             22659..24605
                     /gene="gyrB"
                     /locus_tag="ACHALG_001301"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFN2586723.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24625..27096
                     /gene="gyrA"
                     /locus_tag="ACHALG_001302"
     CDS             24625..27096
                     /gene="gyrA"
                     /locus_tag="ACHALG_001302"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFN2586724.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            27332..27631
                     /gene="rpsF"
                     /locus_tag="ACHALG_001303"
     CDS             27332..27631
                     /gene="rpsF"
                     /locus_tag="ACHALG_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFN2586725.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27681..28193
                     /gene="ssb"
                     /locus_tag="ACHALG_001304"
     CDS             27681..28193
                     /gene="ssb"
                     /locus_tag="ACHALG_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFN2586726.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            28219..28455
                     /gene="rpsR"
                     /locus_tag="ACHALG_001305"
     CDS             28219..28455
                     /gene="rpsR"
                     /locus_tag="ACHALG_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFN2586727.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28598..30571
                     /locus_tag="ACHALG_001306"
     CDS             28598..30571
                     /locus_tag="ACHALG_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HFN2586728.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGESAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30577..31029
                     /gene="rplI"
                     /locus_tag="ACHALG_001307"
     CDS             30577..31029
                     /gene="rplI"
                     /locus_tag="ACHALG_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFN2586729.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            31303..32670
                     /gene="dnaB"
                     /locus_tag="ACHALG_001308"
     CDS             31303..32670
                     /gene="dnaB"
                     /locus_tag="ACHALG_001308"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFN2586730.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            33450..34742
                     /locus_tag="ACHALG_001309"
     CDS             33450..34742
                     /locus_tag="ACHALG_001309"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFN2586731.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT        10625 a         6783 c         6908 g        10593 t
ORIGIN      
        1 gtggagcaag ctccggtgga aaaagaagcg ttcgacttgc atgtattagg cacgccgcca
       61 gcgttcgtcc tgagccagga tcaaactctc ataaaaagtt cgaacaatct tgcgattgtt
      121 aagctcaatt tgtttgctag catattgctt gcttgttaaa aatgtttgtt gtcttgaatt
      181 gaatcaagac gccctacaca tttggtttgt cttactttgt tcagttttca aaggtctact
      241 tcatcaggag tgtttcccga ttcgtttttg gctgccgtag caacttttat atcatatcag
      301 agagacaagt aattgtcaac tcttttttaa aagttttttc gatttcgttt ttcgaaatct
      361 tccagcaact catttatcat aacttatcgt ttgttatttg tcaagaactt ttttgaagtt
      421 tttttcgata gaatcaagct tcgcaagtgg ttctttttta acgactcagt tatcttatca
      481 tgtcatttcc aacatgtcaa caacttattt tttattattt aataagttat tggtgttctt
      541 ttgaacaaat aataatatat caacatcaca tgtgaaagtc aataagaaaa tgcttattta
      601 tcaacatttc tagcttacat gatcattggt tagttctggt cctttttcgt tcgtctttat
      661 aactccgaga gacgtaaaat ccttttgttt tcagtttaat gttcatgttc ttgtcttatt
      721 cacaccaact agatgtctca ccttctaaaa ttttccttaa caaagtgctg cttttatcta
      781 tttctgcttt tttttgctag aataatagta acaatatgac tctggaggat ttatttatga
      841 aaattttgat tgctgacgat gataaagaaa tcgttgaatt attaagtatc tatgttcaca
      901 atgaggggta tgaagcagta aaggcttatg atgggaaaga agcattatct aaaatcagaa
      961 caatccctga tattgaatta cttattttag atatcatgat gcctgagatg gatgggatgc
     1021 aagtagtgaa agagctacga aaagaatccc aaataccaat cattatgctg acagcaaaaa
     1081 ctactgatat ggataagatc aaaggactgg tcgctggtgc agatgattat gtgaccaagc
     1141 catttaatcc gttagaagta atggctagag taaagtcttt attacggcgt acaaaaatgc
     1201 agattgcccc agatcagcct gatgtattag aagtaaatgc attaatcatc aataaagact
     1261 ctcatgaagt taaaacaatc gatgggaaag aaatccagtt aacagctttg gaattcggta
     1321 tcctctactt gcttgctagt catcctaatc gagtatttag cgcggatgaa atttttgagc
     1381 gtgtttggaa acaagaaagt attgtctctg ctaaaactgt catggttcac gttagtcatt
     1441 taagagataa gattgaagaa gcaactggtg gtgaaaaagt cattcaaacc gtttggggag
     1501 tgggctataa aattgacgca agatagtaaa cgagcaactg aaaaacgccg tcggatcaat
     1561 ttgacttcaa aagaaatcag cgaattgtta gcggaaggaa tcatcacgat tatccttttg
     1621 cttctattaa atgtttctat tctggttgtt atcagttcgg tgatcaacag ttcaccttct
     1681 ttgacgaatg cgatttggga ttcaaagaat atttttgcag aacgattgaa cactgatttg
     1741 ttctggaacg gccggaattt tattattccc tttttctttc tattagatat tggtgttttg
     1801 tattggcgct tgattcgaag atatcgccaa atgcaattgc ggcatatcat cagcgaactt
     1861 cactacatcg caaacggaaa ttatgatcat cgtatccctt ttgaactgag tggtgactta
     1921 agccgagtgg taacaagtat caatggattg gtggatagta ccgtagcagc gattgaagac
     1981 gaacgtaaaa tcgaaaaatc aaaagatgaa ttgatcacga atgtcagtca tgatatccgt
     2041 accccactca cgtccatcat tggataccta ggactgatag aagatggcca gtatcactca
     2101 gaagaagatc ttttaaagta tacgcatact gcttatatca aagcaaagca gatgaaatct
     2161 ttagtggacg acttatttga atacactaag gtacgccagc ctgctgtccc ggtgaatttt
     2221 tctgcctttg acatgatcca gttgatcgaa cagttagcag ctgattttga gttggaagct
     2281 tctaaaaaaa atatccaaat acttgttcaa tcaaaagtag actcccttat catggatggc
     2341 gataccgaaa aacttgtacg tgtcttcaat aatcttttga cgaatgcgtt gaaatacgga
     2401 aaaggagcaa cgaagatcgt aatcgaagta gaacgtatcg gttcagaagt tgttgcaaca
     2461 gtcaaaaata acggagcaat gatcccacaa caggcaatcg ataatctatt cgaccgtttt
     2521 tatcgtgtag aagaatctcg ttcacaagca actggaggaa ctggacttgg tttagcaatc
     2581 gctcaaagca ttgtggcttt gcacggcgga tacatttatg caaaatcaga taagcaatgg
     2641 acttctttca tcatgcacct tccaatcaag aaaaatgaaa aattacctat tgatacacag
     2701 gaagttattg acgaaagctg aaatattcgc tattcttatt acaattatta gaataaatga
     2761 aaaacacaga acccagtaca ctttattctt tttcagagag ctgatggcag gtgtaaatca
     2821 gcaaaggata atttggaagt tccagttcgt ttacgtgccc tttcgaaata agaaaggtcg
     2881 gttcaacagc cgttatgtgt tcaacaagtg cagctgacgc tgaacttggg tggtaccgcg
     2941 aatttcagac tgattcgtcc caagaataat gggatggatc agtctttttt tattttcaaa
     3001 aaggaggaaa tcatatgtta gatgtaaaaa tgatgcgaca aaatattgaa gaagtacaag
     3061 aaaaattgat gactcggggc gtaaaaaaag aaacattagc tgaattcatg gaactagatg
     3121 aaaatcgtcg taagctacta gtcaaaagtg aagaattaaa aaaatatcga aatgatgttt
     3181 ctgctgagat cgcccaattg aagcgtgcaa aagaagatgc ttctgctaaa atcgctgaaa
     3241 tgaaagaagt cggtggcaac atcaaagcat tggatactga aattgctgaa gtggatgaaa
     3301 aattgaaaca aattgcaact actttgccaa accttcccca tgattctgtg ccagttggaa
     3361 cagatgaaga ggacaatgta gaaatccgtc gttggagcga accacgtaca tttgcttttg
     3421 aaccaaaacc acactgggaa attgcagaaa atcttgatat tttagatttt gaacgtggcg
     3481 caaaagtagc cggtagccgt tttgtctatt acaaaggatt aggtgctcga ttagaacgtg
     3541 ctgtctacaa cttcatgctt gatcagcata tttacgaaca cggctatact gaaatgatca
     3601 ctccttatat cgtcaacagt aaagcgatgt ttggtacggg acaatttcca aaattcaaag
     3661 aagatgtttt ccaattggag aatactgatc taacattaat cccaacagct gaagtaccat
     3721 taactaacta ttataatgat gaaattttag atggtgcaga tttacctatt tatttcactg
     3781 cactaagccc tgcttttcgt tctgaagccg gaagtgctgg tcgggataca cgaggactga
     3841 ttcgtcttca ccaattcaat aaagtagaaa tggtcaaatt ttcggatgta gagcattctt
     3901 acgaagaatt ggaaaaaatg acagctgatg cagaagatat cttgaaaaaa ttgcatttgc
     3961 cttatcgtgt gatggcctta tcaactggag acatgggctt ttctgcagct aaaacttatg
     4021 acttagaagt atggatacct gcacaagata cttatcgtga aatcagttct tgttctaact
     4081 gtgaagattt ccaagcacgc cgcgcaatga tccgttatcg cgataatgat ggaaaaatcc
     4141 actatactca tactctgaat ggttcaggac ttgcagttgg ccgtacagta gctgccattt
     4201 tggaaaacta ccaaaatgaa gatggttctg tcacagttcc agaagcgctc gttccttata
     4261 tggggaatct gaaagttatt aaataaagag gctgtgaaaa agccgcttag tttcgagtat
     4321 taagaaaaat tttgtgaaaa tggctttcca catttttgca taaattggac ttaataccga
     4381 gaaactggct tttgaacacc atttatcgag aggttgtgaa atcagcgaat taacctgagt
     4441 attagagaaa aaaatggaaa aatagtccct catattttac cgtttgttga actaataccg
     4501 agggtcagct gattgaacac cgtttagtgg agaggtcgta agcctacccc ccagctccta
     4561 gttacaagga caaattcaaa aaacagctct tcatatttta tgaatgttgt acttgtgatt
     4621 cggaggtggc aagggaacac tgtttattca aaaagaggct gggacagaag cgtttaactc
     4681 caagaaataa gaagaaattc acgaaaattg cttttcaaat ttttgtgaat ttcttcttat
     4741 ttccgaagga gttgcttcta cttccgccgt ttatacgtgt ttagagcgtg agacaaaagt
     4801 gttctttact tttgtcccac gctctttaat gtttttaaga ttcaactgaa tagtttggtg
     4861 cttcttttgt gatttgtaca tcatgagggt gtgattcttt taatccactg ccgctcattt
     4921 ggataaattg cgcttcatca cgtaattgct gtagattggc tgccccaaca taacccattc
     4981 ctgaacgcaa accgccgatc atttggaaaa taatatctgc aacacttcct ttataagcaa
     5041 cccgaccttc gattccttct ggtactaatt tatttgcttc attgacacta ccttggaagt
     5101 aacggtcttt tgaacctttt tccatcgcgc ctaatgagcc catacctcgg tatgttttga
     5161 atcgacgtcc ttggaagatt tcgaattcac ctggtgattc atctgtccct gccaacatac
     5221 ttcctagcat cactgcatgt ccgccggcag ctaatgcttt gacgatgtca ccagaatatt
     5281 tgatccctcc gtctgcaata atcgctttgc catattgacg agcaactgat gctgcatcat
     5341 aaatagctgt gagctgaggt acacctacac cggctaccac acgtgtcgta caaatcgatc
     5401 ctggtccgat accaactttt acgacatcta cacctgcatc atataaagct tttgtcgctt
     5461 cagccgtcgc aacgtttcct gcaatcaatg ttgcatctgc aaaagttgaa cggatttctt
     5521 gaatttttcg gatcacacct gcactatgtc catgagctgt atcgatgatg attgcatctg
     5581 cccctgcttc taataaagca ttcgcacgct caaatgtatc gcttgttaca ccaacggctg
     5641 ctgctacaag caatcgacca tgttcatctt ttgctgcatt tggaaattcg atcactttct
     5701 cgatgtcttt gatcgtgatc aatccgctta aacgaccttc gttatctacg attggtaatt
     5761 tttcgatttt atgtttttgc aaaatctttt ctgcatcttt tagagaggta cctactgggg
     5821 cagtcactaa gttgtctttt gtcatcacat cagcaatcgg catgtgataa tctgttacaa
     5881 aacgcatgtc gcggtttgta attatcccta ccaattttct gttttcaagt gtttcaacga
     5941 ttggcacacc actgatacga tatttgctca tcaattcttc tgcgtctgct acaagatgct
     6001 gtggcgttaa gaagaacgga tcaatgatta cgccactttc agaacgtttg acttttctta
     6061 cttcatccgc ttgctggctg atcgtcatgt ttttatggat cacacctagt ccgccttgtc
     6121 ttgccatagc aatggccatc ttgctgtcgg taacagtatc catacttgca ctgatgattg
     6181 gaatattcaa cttaatattt ttagctaatt gtacgctcat atccacttcg tttggtaaca
     6241 cgtgactttc tgctggtatc aataagacat catcaaaagt aaatcctttt ttcgtgaact
     6301 ttgtttccca gttggacatt tttagtacca ctcctctaat ttagtttatc tattaaaata
     6361 attgattttt tcatttaagt caatgatcta cttggaatca agttagcttt ctcatagaat
     6421 accaatataa agcgtttaca tctgtgtata ttaaaagaga ttgtaaaaaa gctgtcctgc
     6481 atcaagtaat aagaacagcc aatcaaaaac agcttctcat gtttttgaat tggttgggtt
     6541 tattaccgca gatgcaaact tttgaacacc gtttagtgga gaggctgtga aaaagctgct
     6601 tagtttcgag tagtaagaaa aattttgtga aaatggcttt ccacattttt gcataaattg
     6661 gacttaatac cgagaaactg gcttttgaac accgtttatc gtaaaaaggg actgtgacaa
     6721 gacttttgtc acagtccctt actattattt aactatggat aaacaaaatg aggttcttta
     6781 ctacccctag atatctagca aaatagctga tctttttgct agtttatctt cttgggacgg
     6841 ataaacttcc aataatcccg tattttttct tcaagtagta acggattgct aaagcagttg
     6901 ccccgataat gataactact actggatcta atactggatt gataactggt ggcaatagtg
     6961 ttgtcgcaga aaacagtacg acccaaagaa gtgtcgttcc acctaggata agcaatgtct
     7021 tacccattcc tggtttcttg cttcgatctg ctccgggata ttcatattga tagatgtatt
     7081 tgtacatcat ataaaagacc cagccaccga ttgcagaacc tagaacgagt gtagaaaagc
     7141 cataagctgg cgttgttcca cgtgaaaaca atccggtaat cgcaatcatg atacttaata
     7201 gaccaagcaa cagcaacgta ttatctaacc acatcaataa aggggtggat gaatttttta
     7261 cttctgctgg cttattgatg attgcttccg ttctttcaga gactgtccca aacaattgac
     7321 gggctgttct tccgcctttt tgttctgcaa ctaaagtagg cagaatgtca tgaagggctt
     7381 ttactttttc ttcttcagac aaattcgctg cttctaaaga ttttttcaaa tcaaaaatgt
     7441 actgttgatt tcttttggtt aattttgttt ctaaactatt gttttctttt acgattgctc
     7501 gtaattgttc tggttccatt atctaatagt cctccttagc cgttcctttt ctcaacggtc
     7561 gtcacaaaag acattctacc atattctaca gctagtttga tagccctttt acacattaaa
     7621 gcggaataac attacgtcgc cgtcttgtac gacataatct tttccttcta atcgaacacg
     7681 tccagcttct tttgctgcat gcatgtttcc gtaatgatcc aagtcttcaa aagagacagt
     7741 ttctgcacgg ataaatccgc gttcgaaatc tgaatggatg attcctgctg cttgtggtgc
     7801 tttgattcct tttttgaatg tccaagcacg tacttcttgt tccccagcag taaagtaagt
     7861 agctaagcct aacaaatcat aagcagcacg gatcaactga tccaaacctg attcttcgat
     7921 tcctaaagct tctaaaaatt ccgctttgtc ttcttcatct aattctgcga tttcttcttc
     7981 tgcacgagca ctgaccacga tcacttctgc attttcttct gcagcaaagt tgcgcacttc
     8041 ttgtacataa tgattcgcgt ctgcatctgc tacgtcatct tctgagacat tggctacata
     8101 aagaattggt tttgtcgtca ataaaaatag tgatttaacg attttttgtt cttcttcagt
     8161 aaattcgatc gttcgggcag attttccttc ttccagtaca ggtttgattt tatccagtac
     8221 agctagttct gccactgcat ctttatcttt ggttttagca acttttgcta cacgagtata
     8281 gcgtttgttg atagattcta agtctgctaa cactagttcc aagttgatcg tatcaatatc
     8341 ttctagtgga tcaacacgtc cttctacgtg agtaatatta tcatcatcaa aacagcgaac
     8401 aacgtgacaa atagcgtcta cttggcggat atggcttaag aattgatttc ccaaaccttc
     8461 tcctttactt gcgcctttta cgattccagc aatatctgta aattcaaaag tcgtcggaac
     8521 agtttttttc ggatgtacaa gttctgttaa gcgttgcaaa cgccagtcag gtacttctac
     8581 catccctacg ttggggtcga tcgtcgcaaa gggatagttt gctgcttctg ctcctgcttt
     8641 tgtgatcgca ttaaacaaag ttgattttcc tacgttgggc aacccaacaa ttccagcagt
     8701 caatgccata aatttattca ctctttctct gcattttttt caattacttt ttttaatttc
     8761 ttttcaaaat ctcgacgggt catcatgacg atatgtccgc aattcataca tctgattttg
     8821 atatctgcgc ccattcgaat gacttcccag cgattcgtct ggcaagcgtg aggttttttc
     8881 atttcaacga tatcgtgcaa gtcgtacata aaaatagcct cctttttgtt tattcttcat
     8941 caaattgaat attcaagata tcaagaattc gagtcaaatc tttttgagaa agatattcaa
     9001 tctcgatctt acctttccca tttttttctt ggatagctac acttgtacca aatttatcca
     9061 tcaaacgatc ttcgctttct cggatataat atggtttttc ctttgctagc cttgggattt
     9121 ttttcttttc ttttccatca ttttgattta actcagaaac aagctgttcc agttggcgga
     9181 cagttaaatt ttcagttact gcacgattgg ctaatttcaa gatcaatgtt ttatttttta
     9241 aacctaacag cgtacgggct tgtcccatag ataaacgctg atcttgtacc atttccttga
     9301 caagatccgg taaagaaagc aagcgcaaat aattggcgat ataaggtctg ctctttccta
     9361 aacgactagc tacttctgct tgtgttagtt tcaggttttt catcagcatc tcatatgctt
     9421 ctgcttcttc taacggattc agatcttctc gctgcaagtt ttccagcact gctacttgca
     9481 tcatcgcttc ttcgtcaaag tctctaacga tagcagggat cgtttctttt tcagctagtt
     9541 tggacgctcg aaaacgtcgc tcccctgcaa taatctcata tccttttact gcggaacggc
     9601 gtacgatgat gggttgaaag acacctgatt gttcaataga attagctagt tcctgcaaag
     9661 atacttcttc aaatgtttta cggggctgat acggatttgg tcgcagctca ttcaatggta
     9721 gctggacaac ggtatcatct tttacatcca ccgtctctaa gttttctaaa tcttgaaaca
     9781 gtgcatcgat tcctcggcct aatcctttac ttttcttcac gacttaccac ttcctttgcc
     9841 aatgcttggt agacttcagc tcctcgagaa cgtggatcgt agtcgatgat cggttttcca
     9901 tgactaggtg cttctgataa acgaatattt cttggaatga tcgtttcgta aactttttcg
     9961 cggaagtatt tacgtacttc ttctacgact tcatttccca gatttgttcg tgcatcatac
    10021 attgtcagta agactccctc gatctctaat tctggattaa agtgtttttg gactaaacga
    10081 accgtattca gtaattggct taatccttcc aatgcgtaat actcacactg aacaggtatc
    10141 aaaatcgaat cacttgctgt aaatgaattg attgtcagat ggccaagaga aggcgggcaa
    10201 tcgatcaaaa tataatcata ctgactgctt acttctgcta aagatccttt taatcttgat
    10261 tctcttgcca tcattgacgt caattcgatc tctgctcctg ctaattggag agtcgcaggc
    10321 acaatactca aattttcgtg ttcagtgatc aaagttgcct catcaattgg caattcattg
    10381 actagaacat catagatgtc tcttgtcaca tcaggttttc tgatcccgac accactggta
    10441 gcatttcctt gtgcatccat atccactagc aatacttttt tgccaaggct agcaagacaa
    10501 gcacctagat tcaccgtcgt cgttgttttg ccgacgccac ctttttggtt cgcaacagat
    10561 attatttgtg ccatctcttc tcctcctatt tgattggctg cttattaggc agtccgggct
    10621 ttcttgggta tttctttggg gttggtttct ttttttgaat aacgatgata tgccgttgat
    10681 cagagacatt cggcaaggta accgcctctt ctttgaggaa tttaccacct aataaagcaa
    10741 tcgcttgttt tgcttctgtc aattcctctt cactttttgc tgctttcaat gcataaaaat
    10801 agccttcttg tttagccaag ggcaaacaca attccgctaa cacattcaaa cgagcaaccg
    10861 cacgagcagt gacaaggtca aatgatgcac gaaagtgagg attttgccca aatgtttctg
    10921 cccggtcatg atataaggaa gcatcgattc ctaataatcg ggttaactca gataagaagg
    10981 ttattcgttt attcagagaa tcaacgatcg ttatctgcag ttctggaaag gcaattttta
    11041 aaggtatgct aggaaaccct gcccccgaac caacatcgca taaagataaa ggttgcgtaa
    11101 agtctacttc aaatgctaat gtaagtgaat cgtaaaaatg cttcagatat acgtcttttt
    11161 catccgtaat agctgtaagg ttcatctttt cgttccattc gactaatgtc cgataataaa
    11221 tagcgaattg attcatttga tgatcggtaa gctctatccc tttttctgcc aaggattggc
    11281 gaaattcttc tggtatcata aatattcctt tcattgtgaa acagcaattg tttcacagat
    11341 tctctcccta ctttaacgca aaatctctag acatgcaatc cttccatatg gattcttaaa
    11401 aatttgaaaa gtaagaaaag ccgatagact ttttctttct agtatgactc ataaaatata
    11461 cacagaagag agaactagct ttgagcaatt tctgcaaaaa ctagttctct cttctattta
    11521 ttattacgta ttacgctggt tcactgactt tcgcaatctt cccttgttcg atatagacca
    11581 ttaggatact taggtctgca gggtttacgc cactgattcg gctagcttgg gcaattgttt
    11641 ctggttggat tttctgcaat ttttgttttg cttctgttgc taatccgttg attgctgcgt
    11701 agtcgatgtt ttctggaatc tttttcgctt ccatgcgttt taatttttct actttttcca
    11761 tggctttttt gatgtagcct tcatatttga tttggatttc tacttgttcg atttcttttc
    11821 gtgtcaattc ttcattttct ggaataaacc gtagaagttc tgcataagta atctcaggac
    11881 gtcttaaaaa gtcgcttgct agtacaccat cttttaatgg tgccgcatta atcgttgcta
    11941 aaaagtcttg gacttcttct gttggtttga ttcgtacatt catcaaacgt ttgatttctt
    12001 gttcaactgc ttcttttttc ttcaaataat tcttgtattg atcttctttt actaagccta
    12061 cacggtgacc catttctgtt aatcgcaaat ctgcattatc atgacgtaaa agcagacggt
    12121 attcagcacg tgaagtcagc aaacgataag gttcgttcgt tccttttgtt accaaatcat
    12181 cgatcatgac accgatgtaa ccgtcacttc gtttgatcac taaaggttct tttccctgaa
    12241 ctttcaaagc tgcattgata ccggcaatca gcccttgtcc agccgcttct tcataaccgc
    12301 ttgtaccatt tgtttgtcca gctgtataaa gattttcaac gactttagtt tctaatgtcg
    12361 gacggagctg gtgtggctct actacgtcat attcgatagc atatcctgta cgcatcattt
    12421 ctactttttc taatccagca atagaatgaa gcatgttgac ttgaacatcc tctggtaaag
    12481 aagtagataa accttgaaca tagacttctt ccgtatttaa gccttctggt tccaaaaata
    12541 attgatgacg tggtttgtct gcaaaacgca cgatcttatc ttcgatggat ggacaatagc
    12601 gagcgcctac cccttcaacg atccctgtaa acattggggc acgatgtaag ttgtctcgaa
    12661 taatttggtg agtcgtctca cctgtatagg tcagccagca agattcctga tctagtttat
    12721 aggcactatc tggtgtgctg aagctaaaat gattcggctc tttgtcacca ggctgctctt
    12781 ctgtaacaga atagtcgatc gtacttgatt ttactcgagg cggtgttcct gttttgaacc
    12841 gttcgatagc aaagccaagt tcttttagat gattcgctag tccaatagaa ggttgagagt
    12901 tattcggacc tgatgaatat ttcaattcac cgataatgat ttctccgcga agtgctgttc
    12961 cggctgtaat aacaactgct ttggcccggt agctcgctcc tgtggacgtc actactccat
    13021 gacaaacacc atcttcgacg atcagttctt cgacgattcc ttgtcttagc gtcaaatttg
    13081 gttctttttc gatcgtatgt ttcatttctg ttgaataagc atgtttgtct gcttgtgctc
    13141 gcaatgctcg gacagctggg ccttttcctg tgttcaacat acgcatttgg atataggttt
    13201 tatcgatatt cttacccatt tctccgccta acgcatcgat ttctcgaacg acaacgcctt
    13261 tagctggtcc gcctacagaa ggattacatg gcatgaatgc taccatgtcc aaatttattg
    13321 tcagtaataa tgtttttgat cccatacgtg cagcagcaag tgcagcttct gaaccggcgt
    13381 gtcctgcgcc aacaacgatc acgtcatacg catctgcttc ataatgttcc aatcgtttgt
    13441 tcctcctttt attttcctaa acaaaattga ctgaataatt gagtgattag ctcatcttgt
    13501 acactgtcgc cgacaacttc tcctagatag tcccagcaac gcgtcatatc tatttgaacg
    13561 agatccacag gcattcctga atcaatacct gcaatgactt cttcaagtga gagggaagct
    13621 ttttctaaca acgcgatatg gcgggtatta gaaacataag tagcgtctct ttctcctgtt
    13681 tctccgctga agaataaatc agaaatagct gattccaaac gatctagtcc gtcgttttta
    13741 gctacggaaa ttgaaaaaac tggttcattt tcgatcaatt tcttcaattt ttcttgttcc
    13801 agctgtgctg gcaaatctgt tttatttaga agaatgatcc gtttcaatcc acttgtcgct
    13861 tccaataatt gttcatcttc tgctgtcagt ggttcgcttt ggtttagaac aagtaagatc
    13921 aagtctgctt ctgctaatgc ttttcggctt ctttcgaccc cgatccgttc caccacatct
    13981 tctgtttccc gaattccagc tgtatcgatt agcttgagcg gtaccccccg aacattgaca
    14041 tactcttcga ttacatctcg agtcgttcct gcaatatccg taacgattgc tttttcttct
    14101 cttaacaaat ggtttaacaa acttgatttg ccgacatttg gccggccgat gattgccgta
    14161 cttaaccctt cccgcagaac cttcccttgc ttggatgtag caagtagcgc ttggattctt
    14221 tgttggatca tctgcgcttt ttcaagcaat aacttcgtag tcaattcttc gacatcatca
    14281 tattctggat aatcaatatt tacttctact tgtgctaatg tctctaaaat ctcttgtctc
    14341 aatgagcgga tcaaagcaga cagattgccg tctaactgat taagagctag tcccattgct
    14401 ttgtctgttt ttgcccgaat caaatccatc acagcttctg cctgtgaaag gtcaacacga
    14461 ccattcaaga atgctcgttt tgtgaattcg cctggctcag ctaatcttgc accttctctt
    14521 aacaatagct gcaaaatttg attgactaca acgatcccac cgtgacaatt gatttctact
    14581 acatcttccc gcgtaaatgt ctttggtgct ctcatcacgg agaccatgac ttcatcgaca
    14641 agctgattgc tcttgggatc aacgatatgt ccataatgga tcgtatgact tggtacttct
    14701 gataatcgtt tgtttccaga ttgataaact ttatctgcta gttgcacagc ttgatcgcca
    14761 cttaaacgaa cgatactgat tgcaccttct ccaggtgggg tagaaattgc cgcaatcgta
    14821 tcaaattcta aagtaatttc agccattttt tcacttcctt tttcattttg cgcacaaaaa
    14881 aagtgcccac tccacccttt atgcacaata aacggtgaac ttcgcacttt gactgcttgt
    14941 tcttgattaa attctggtca tagattatca cacttgctat aaggaatcaa gtgtttaaaa
    15001 caattagatg aagttgagcc tgttctgctt ccattacctt atatttaatt gtccttggat
    15061 gtctctttgc gttctttggt ttattcgaag agaagatact gtataaacaa ggctttatct
    15121 ttcttaaaaa aaacggtaca ctaaatggta gcgctatcta aactatacac ttttgaaagg
    15181 agaaaatatg tgagcccgtt cagatctgct gcattggaag aagaaagtga ccctcggatc
    15241 aacgatgtcg ttttatctac agggaatatc aaccagaaat atatcgtccg cgatatcgtt
    15301 tttgttgtgg aatgcttaga agctgatttg tttgacccat cttttcccaa agatgacctt
    15361 ttcttaacaa cgaagaggaa attaaagacg aaggtacttg aatatggtgc gaatgcagta
    15421 atcaactgtc attttgagta tcagcaaaaa aaacgcgacg gaaagtattt tctggaactt
    15481 tttgcttatg gaacagtgat ccaatttatc caaacaacga ttggttaaat gaaacagaca
    15541 aaaaaagcgg atcaaaactg aagacagttt cgatccgctt ttttctatca aagttagtga
    15601 ctacttcatc gtatactctg ataagaatga tttctagtaa aatatacttg gtttgaaaaa
    15661 ctttgtcaac gtgatttatt caaaaagtaa tggaatcaat aatatttttt agcaggttct
    15721 actactaaat aacgataagg ttcctcacct tccgaatgtg tttttacatg ttcatctttg
    15781 cttaatgcag catggatttg tttacgttca aatgcaggca ttggttctaa gaaaacaggc
    15841 tgccctgtcc gttttacttt ttcagccgtg cgatcagcta aacgctgaat gatctcttgg
    15901 cgcttttcac ggtaattgcc tacattgact actacagaca gcttatttct tgctaaacga
    15961 tggatgaaga cttgtgccag atattgcaat gcattcagtg tttttccatg tttcccaatc
    16021 agtatgcctt gcttttgtgt ttctaaatgg aaaacaacca tattatttga ttctctttcc
    16081 attttgacaa gagctggagc atcaagctgt tttgaaattt cagtaagata aactgccaat
    16141 tccgttaaag ctgcttcatc acctagctca cttacttctt ctgttttttc ttcaagaatc
    16201 gtttcgttga ctacctcgat ctcagctgtt ggttcttctt ccaaaatttc ttctacagtc
    16261 tgttctactg tttcagaaac tttttcgctg atacttggct ggatcgaaag ccgtgcattc
    16321 tttcgaccca ttcctaggaa accttttttt ccttcttcca gtatttcgat ttcgacttgg
    16381 tctttggcca aacccaaatc agccagacct agttggacag cttcgtctat tgttgcagct
    16441 tcataaatcg gcattagcat tacctcccct attatttccg ttttttctta ggattcattg
    16501 ctttcttaag agcacgttct ctttcgcgga ttctccgtgc ttcttcttca cgctcttttc
    16561 gaattttgaa agggttgttg atcagcagcg tttgtacaac ttggaaggca ttggaaacta
    16621 cccagtataa tgaaagaccg ctggctaaat tgacacccat cactaaaatc attacaggca
    16681 tcacgaaatt catgatcttc attgatgcat tcgattcaat ttgactcatg ctagataaat
    16741 acgtacttgc aaaagtaaag attgctgcta aaatcggcaa aataaatgtt ggatccgcgt
    16801 tccctaagtt caaccataag aaatgccctt gtttcaactc tggtacacgt gaaatagatt
    16861 gccataacgc catcaagatt ggcatttgta ctaataaagg aagacaacca gcatatggat
    16921 tgacgccatt ttctgcatat aaacgctgct gctcttctct taacttactt tgtgtttccg
    16981 gatctcttga actatattgt tgctgcagtg cttttaattt cggttgtaat tcttgcgttt
    17041 tacgcatgct ctttgtttgg aaatgcatca acggcaatag gatcacgcgg atgatcagtg
    17101 taaataaaat gatccctatc cctgcattac caaatgacaa cgccttgatc gcctcagcaa
    17161 agtaataaac aatataacga tcccaaattc ctgtactctg tgcgctgact tccccagttc
    17221 cgcaaccgga taagacgatg accaaactaa cgataccggc catcaaaagt aaacgtttgt
    17281 atttcttcac ttactctttt cctctctgtc gattattttt gcaagtttta agacgtggac
    17341 taaattggag cgaacctcat cataagtcaa ccccttcaca cttggtctag caatcacaat
    17401 aaaatccaac tccggatcaa tttcttcttt tatagaataa actgctgctc ggatttttcg
    17461 tttgacttca ttgcggttta ccgcgttgcc gacctttttt cctacagata aaccaactcg
    17521 aaaatgaggc tgtcccggtt tttccaaacg gtaaacgacg aatttgcggt tagcaaaaga
    17581 atctcccgta tggaacacgg cttgaaactc tcgttctttt tttactcgat acgcttttct
    17641 cattagtttt ctttcctttt ctatctctca cttacgctca taatcatacc ataattacga
    17701 ttgtcctttc atcatttccc taaaaaagag tataaaaaaa accactgaaa ccccagtggc
    17761 ctaagctgaa agaacttttc ttcctttgcg acgacggcta gctaacacac gacgtccatt
    17821 tttagtactc atacgtttac ggaatccgtg aactttttga cgtttacgtt tatttggttg
    17881 gtatgttctt ttcatatatt ccacctccaa aatgttctta tctattttat aaacacagac
    17941 atactttgat agtataacga caaagtagtg attttgtcaa tataaaatag aagatcgatt
    18001 gttaaaactt atccacaaga ttattttttt ctatgggaat cctatgtgga aaaagttatg
    18061 cacagggatt atccacagat aataaaaggc tggttttaca cagaaaacac acgtgtaaaa
    18121 aaagtaatcc acaatcaggc gatttcctgt gtattccgtc tcctaactat tggtatatct
    18181 gtatttttta tccacagacg caagaggaaa tctattggtt tttacgagtt ttccacctgt
    18241 gcattagtgg tgtggaaaac tttttttgac aactgtgttt ttctcttttt tctttttgtc
    18301 gattgtgtgg ataattgtaa ttaaaaaatt tttttcttcg gtttctgtgg aaaactattg
    18361 aaaaaaatgc taaattagta ctagttattc actttttaat gaaggaggag gccttatatg
    18421 gtatccctcg atgctttatg gaatgaatta aaagcaacat accaaaagga tttatcgcct
    18481 gctagctata atacatggat cgaaacagcg aaccctcgaa cccttgatca aaaccagctc
    18541 gttgttgagg ttcctagcaa gattcataaa gaatattggg aaaaaaacct ggcgacaaaa
    18601 atcgttgaag tcggttatat gttatcagga aatgagatta tccctcgctt tattactggc
    18661 gaagaagcag agcaagaaga agttatagaa gaaaaaaatc caaaagttgt ggcacctagt
    18721 ccgctaaaaa aagccatgct gaaccctaaa tatacctttg atacgtttgt cattggtaaa
    18781 ggaaaccaga tggctcatgc agcagcgctc gttgtagcag aagatccagg ttctatttat
    18841 aacccgctct tcttttatgg aggcgtggga cttggtaaga ctcacttgat gcatgcaatt
    18901 ggtcatcaaa tgctgcaaag tcagcctaat gccaaagtaa aatatgtaag cagtgaaact
    18961 tttgctaatg atttcatcaa ttcgatccaa aacaaaacag cagaagagtt ccgtcaagaa
    19021 tatcgaaatg ttgatttact gctagtggat gacattcaat tttttgcaga aaaagaagct
    19081 acgcaagaag agtttttcca tactttcaat gccttgtaca atgaagggaa acaaatcgta
    19141 ctaaccagtg atcggttgcc aaacgagatt ccgaaactgc aagaacgcct tgtttctcgc
    19201 tttgcatggg ggctgtctgt tgatattaca ccgccggatc ttgaaacaag aacagcgatt
    19261 ttacgtaaaa aagcggcggc tgaacgtcta gagatcccag atgacacact aagctacatc
    19321 gcaggacaaa ttgattctaa cattcgtgaa ttagaaggtg cgcttgttcg agtgcaagct
    19381 tttgctgcga tgaatagtga agatatttcg actagtctag ctgcagacgc actgaaaacg
    19441 ttaaaatcag gaaaaggaca tcctcaattg tcaatcctgc agatccaaga agaagtggca
    19501 aagtattatc acatccaatt gaaagatctc aaaggaaaga aacgcgtcaa atctattgtt
    19561 gttcctagac aaatcgctat gtatttggca cgtgagctca cagataattc tttaccgaaa
    19621 atcggagctg aattcggtgg caaggatcat acaacggtta tccatgctca tgaaaaaatc
    19681 cagcagctga tggattctag tgtcagtatg caaaatgaag tatcagaaat caaaaacttt
    19741 ttactaaatt aatttgtcga ttctatcaac tgaaaatagc atgtggataa aagaaaaaaa
    19801 cgttcaaaag ttatacacac tttttccaca gctgtttttt cgtttctctc ttacgttttt
    19861 tgagttttcc acagaatcaa catgccctat tacttttatt attcttttta actaataata
    19921 aataaacaaa cattcaaagg agatacatca tgaaagttac tttaaaccga gctagcttta
    19981 tgcaggaatt gcaaactgtt caacgagcta tttcaagcaa aaccacgatc cctattttga
    20041 caggtgtaaa aatcacactg acacaagaag gtttgacttt gacggggagc aacgctgata
    20101 tatcaattga aacttttttg tctgttgaaa acgaaaaagc aaatatgcaa atcgaatcta
    20161 ctggttccat tgttttacaa gcacgtttct ttagcgaaat cattcggaga cttcctgaag
    20221 aaacatttac tttagaagtt ttagaaaata aacaagtagc gatcacttct ggaaaagcga
    20281 attttatcgt aaatggatta gatgcagata actatcctca tcttcctgtt gtcgaaagcc
    20341 ataaccagat gaaattacct gtacacgtat tgactaaact aatcaacgaa acagtttttg
    20401 ctgtctcaca acatgagagt cgtccaatct tgacaggtgt gcatttcatt ttatctgata
    20461 attctttatt agctgtagct actgattctc accgtctaag tcaacgcgtg attccagtag
    20521 aacaagcggc tgatcatttt gatattgtta ttcctggaaa aagtttgatc gaattatctc
    20581 gctcattaac aaatgaagaa gaaatcgtcg aaatcagcat tatggaaaac caagtgttgt
    20641 tcaaaacaga aacgatgtat ttctattccc gtttgctaga aggaaactat ccagatacca
    20701 atcgtttgat tccttcaagt tttaatacag aggttgaatt ttctgttcct agctttttag
    20761 cggcgatcga acgggcttct ttactttctc atgaaggccg taacaacatc gttcgtttat
    20821 ctattcgtcc agatgcggtt gttttatacg gaaattcgcc agaaatcggg aaagtagaag
    20881 aaagcttgag ttatacagca agcagtggcg atccattaga tatttctttc aatccggatt
    20941 atatgaaagc agcgttgcgt gcatttggag atatgagcat caaagtgaaa tttatctctg
    21001 ctattcgtcc atttacattg gaaccaacgg aagatggcgt tcaatttatt cagctgatta
    21061 ctcctgtacg tacaaattaa caatgatttt caatgttctt gaaaatttaa ttgaaaagaa
    21121 atagaaaaaa taaaatttta aacagaaggc cctaaaatct cttttaaggc tttctgttta
    21181 tttttgttta aatcaccttt tttagattaa atcgtcttat atggaaaaat atatggataa
    21241 atttgtttta ttgcgaaaaa aaaggtataa tagagtatac ttttaataat gaaaatgagg
    21301 gatgggcatt gaaacaacaa gtcattctaa atacggaatt catgacgttg ggccaaatgt
    21361 tgaaagaagt cacagttatc ggtagtggcg gacaagcaaa atggtatttg gcagaaaaca
    21421 ctgtgttagt cgatggcgaa ccagaaaatc gtcgtggacg caaactttat cccggcatga
    21481 tggttgaagt tccagaagta ggaacttttt ttatggtaaa aaaaggaaac gacgataatg
    21541 aggctgaata agctgtattt aaaaaattat cgaaattacg aagagctgaa catcgaattt
    21601 tccaagaacc tcgtcatctt tttaggagaa aatgcgcaag gaaaaacgaa tcttttggaa
    21661 agtatctacg tattagctat gacgcgaagt catcggacaa gtagtgagca ggaattgatt
    21721 ggctggactg acgatcaggc gatgattcaa ggagagatca caaaaggttc atccactctt
    21781 ccattagaaa tcttattatc taaaaaaggc cgaaaaacca agatcaacca tatcgaacag
    21841 aaaaagttaa gtacttacgt gggacaaatg aatgtcattt tatttgcacc agaagatctt
    21901 tctttagtaa aaggcagccc gcagaccagg cggaagtttt tagatatgga attaggccag
    21961 atcaatccaa tctatcttta tgatcttgtc caataccaag caattttgaa acaaagaaac
    22021 cagtacttga agcaattgaa tgaaaaaaaa caaactgatt tgctttattt ggatattttg
    22081 tccgaacaac tagcagcgtt tggcagcaaa gtattgaaag caagagcaca gttcgtcaag
    22141 cgattggagt tttgggctaa ttctttgcat cagcaaatca cccatcaaaa agagcagctg
    22201 gaaatcgagt atctcaccgc agtcgattct ttagaaacac atacgcaaga gcaaatccaa
    22261 gaacaatttc tcgcactttt gaaccaaaat aaaaagaaag acctttttcg gggaacaacg
    22321 acagttggtc ctcatcgtga cgatctttcc ttttttatta atcagaaaaa tgtgcagact
    22381 tatggatcgc aaggacagca aagaacaact gcactaagtg tcaaattagc agaaatcgat
    22441 ttgattaaag aagagacagg ggaatatcca atccttttgt tagatgatgt gatgagcgag
    22501 ctggatgata atcgtcaact ccatctgcta gaaacaatcg aaggaaaagt tcaaacgttc
    22561 ttaacaacaa cgacacttga ccatgtaaaa aataaaatga ctgttgaacc tgagattttt
    22621 tatgttcaac aagggaaact aaaaggagaa gaaactgaat gacagaagaa agaagtttag
    22681 tagaacgcgc taaagagtat gatgccagtc agattcaagt acttgaagga cttgaagccg
    22741 ttcgtaaacg acctggcatg tatatcggtt cgactagttc tgagggtctg catcaccttg
    22801 tttgggaaat tgtcgataat tcgatcgatg aagtattagc aggttttgca acgaaaatcc
    22861 atgtgattat tgaaaaagac aacagtatca ccgttattga cgacggtcgg gggatacctg
    22921 tagatatcca ggcaaaaact ggacgtcctg cagttgaaac ggtctttact gttttacatg
    22981 ctggaggaaa attcggcggt ggtgggtaca aagtttctgg tggacttcac ggagtaggat
    23041 cttctgttgt taacgcgctt tctacacagc ttgatgtcaa agtatacaaa gaaggaaatg
    23101 tgtactatca agaatatcgt cgtggagctg tagtggacga cttaaagatt atcgaacaaa
    23161 cggatcgcca tggaacaacg gttcacttta cgccagaccc tgaaattttt acagagacaa
    23221 ctgagtttga tttttcaaaa ttggctaccc ggatccgtga acttgctttc ttgaaccgcg
    23281 ggatgcgtat atcgattgaa gacaaacgag aagaagaacc agtcatcaat gaataccatt
    23341 atgacggcgg gattaaaagt tatgtcgaat acttgaatgc aaacaaaacc gtgatttttc
    23401 cagaacctgt atatttggaa ggtgaacagc aagatatcgc agttgaagtc tctatgcaat
    23461 atacagacgg ctaccattcc aatatcatga gttttgccaa caatatccat acgtatgaag
    23521 gcggaacaca tgaatcagga ttcaagactg ctttaactcg tgtgataaat gattatgccc
    23581 gaaaacaaaa gctgatgaaa gaaaatgacg acaacttgac cggtgaagac gtgcgagaag
    23641 gattgacagc tgttatttct atcaagcatc ctgaccctca attcgaagga caaacaaaaa
    23701 caaaattggg caattccgaa gtacggacag tcactgatcg gttatttagc gaacatttca
    23761 tgaaattctt gctggaaaac ccaagtgtgg gccgtcaaat tgttgaaaaa ggattactag
    23821 cttccagagc tcgtctagca gcgaaacgtg cgcgtgaagt aacgagacgt aaaggtgctt
    23881 tagagattag taatctgcca ggaaaattag cggactgctc tagcaacgat cctgaaaaat
    23941 gcgaattgtt catcgtcgaa ggagattcgg ccggcggatc agctaagcaa ggtcgtaatc
    24001 gagagtttca agccatcttg cctattcgtg ggaaaatctt gaatgttgaa aaagcaagca
    24061 tggacaagat cttggcaaat gaagagatcc ggtctttatt tactgctatg ggtacagggt
    24121 ttggagaaga ttttgatgta tcgaaagctc gctaccacaa attagttatc atgactgatg
    24181 ccgatgtcga cggcgcgcat atccgtacat tgctgctgac tcttttttat cgttacatgc
    24241 gtcctattgt tgaagctgga tacgtctata ttgctcagcc acctttatac ggggtaaaac
    24301 aagggaagaa tattacgtat gtacaaccag gaaaaaatgc agaagaagag ttgaaacaag
    24361 tcgttgcttc acttcctgcc tcaccaaaac caagcgttca gcgttataaa ggacttggag
    24421 aaatggatga tcatcaacta tgggaaacaa ctatggaccc aagcaatcgg atgatggccc
    24481 gcgtcagtgt ggacgatgct attgcagcag atcagatatt tgaaatgttg atgggggatc
    24541 gagtagaacc ccgtcgtgca ttcatcgagg aaaatgcaca ctatgtgaag aacttagata
    24601 tttaatcatg aaggaggaaa aagaatgagt gaagaaatca gagaaaacat ccatgatgtc
    24661 aatctaacca gcgaaatgaa agactccttc atcgattatg cgatgagtgt tattgtcgca
    24721 cgtgcacttc ctgatgtcag agatggtctg aaacccgttc atcgccgtat tttatacggc
    24781 atgaacgaat taggtgtaac accggataaa gcccataaaa aatctgccag aatcgtcgga
    24841 gatgtcatgg gtaaatacca tcctcatgga gactatgcga tctatgagtc aatggtacgg
    24901 atggcacagc cattcagcta ccgttatatg ctagtggatg gtcatggaaa ctttggttct
    24961 gttgacggtg acggtgccgc cgctatgcgt tataccgaag cacggatgag caaaatcgct
    25021 acagaaatgc tgcgtgatat caataagaac actgttgatt tccaaagcaa ctatgatgat
    25081 acagaaaaag aaccagttgt actacctgct cgcttcccga atcttttagt gaacggaaca
    25141 acggggatcg ccgtaggtat ggccacgaat atcccacctc ataacttgtc tgaggtagta
    25201 gcggctatcg atttgctgat ggataatcct gatgtgacga ccaatgaatt gatggaagta
    25261 ttacctggac cagattttcc aacaggtggt ttggttatgg gaaaatcagg aatccgaaga
    25321 gcgtatgaaa caggtaaggg ttctattacc gtacgtgcaa aagttgagat tactgagatg
    25381 ccaaatggta aagaacggat catcgtgaca gaattgcctt atatggtcaa taaagcaaaa
    25441 ttgatcgaac ggatttctga actgcacaga gacaagcgta tcgaagggat cacagatttg
    25501 cgggacgaat cttcccgtga aggtatgcgg atcgtcattg acatccgtcg tgacgccagc
    25561 gcatctgtta tcttgaataa cttatataaa ttgacatcct tgcaaaattc atttggattc
    25621 aatatgttgg cgatcgaaaa aggtgtgccg aaagttttga gcttgaaaca aattcttgaa
    25681 aactatatcg aacatcagcg cgaagtcatc acaagacgga cagtctttga aaaagaaaaa
    25741 gcagaagcaa gggcacacat tttagaaggt ttgcgtattg ctttggatca tatcgatgaa
    25801 atcattgcga ttatccgcgg gtctaaatca gatgacgaag caaaagcgac gatgatcgaa
    25861 cggttcgatt tatccgatcg ccagtctcaa gctattttag atatgcgttt acgccgattg
    25921 accggtttgg aacgggaaaa aatcgaaaat gaataccaag aactcttaaa actgattgaa
    25981 gatttaactg acattctggc aagaccagaa cgtgttacag aaatcatcaa gaatgaatta
    26041 gcagacttga atcaacgatt tggtgataaa cgacgcacag aattactagt cggtgaggtc
    26101 ttaagtctgg aagatgaaga tttaatcgaa gaagaagaaa tcgttattac attgacaaac
    26161 aatggttaca tcaaacgttt ggcaaataat gaattcagag ctcaacgaag gggtggacgt
    26221 ggtgtccaag gaatgggtat ccacaatgac gacttcgtca aaaatctggt ttcttgttct
    26281 acccatgata cattgttatt ctttacgaat aacggaaaag tgtatcgggc aaaaggatac
    26341 gaaattccag aatacgggcg cacagccaaa ggaataccaa ttatcaattt actaggtatc
    26401 gattcgagtg aaagcatcca ggcaatcatt gcggtgacgg gagaagcaga agaaggccac
    26461 tatctcttct ttacgacgcg tcaaggtaca gtaaaacgta cttctgtcac tgcttttgct
    26521 aacatccgaa gtaatggtct gatcgcaatt ggtttgaaag aagacgatga gctcgtcaat
    26581 gtgctactga cagatggtca atctaatatc attattggaa cacataacgg ctactctgtc
    26641 actttcaaag aagaagcagt ccgcgatatg ggacgtacag cttccggggt tagaggaata
    26701 cgcctgcgtg aggaagatta tgtcgttggg gcagccttaa tgaaggaaga tcaagaagta
    26761 ttagtgatca cagaaaaagg ttacggaaaa cggacaaaag tatcggaata tccagtgaaa
    26821 ggccgcggag gtaaagggat caaaacggcg aacatcactg aaaaaaatgg tccactagct
    26881 ggattgacca ctgtttctgg agatgaagac atcatgctga ttacagataa aggtgtcatt
    26941 atccgtttca acgtatcgac tgtttctcaa actggacgtt caacattagg cgtacgtttg
    27001 atgaagatgg aagctgatac gaaagttgtc acgatggcag cagtcgaacc agaagctgca
    27061 gaacaaacag ttgaagaaca aggatcagaa gaatagccat caagactaat tgtgtaagct
    27121 agcaagaatg accgattaaa aaatctggtt attcctgctg gcttttttga ttagttgaat
    27181 ttctcttaaa atattcgaaa ctgttcattc tgtattgctt ttgaccaaaa tagagtgtat
    27241 aatcagagct tgtgagtagt ctaattaatt agataactct ccttgctctt aggaacctaa
    27301 gagccgaaag tccacaggga ggtgaatatc aatggaaaac acgaaatatg aaatcatgta
    27361 cattattcgt ccaaacattg atgaagaagc aaaaactgct ttaatcgaac gtttcgatgc
    27421 aatcttgaaa gataacggag cagaagttat cgaatcaaaa gattgggaaa aacgccgttt
    27481 cgcttatgaa atgaacggtt accgtgaagg tatctatcac atcgttaaag tatcttctcc
    27541 atcatcagca aatgctgtca atgaatttga ccgtcttgct aaaatcaacg acgatatcat
    27601 tcgtcacatg attgttaaag aagaagctta attgttgttt catgtgaaac aatagaaaac
    27661 gatgagagga gatgactatt ttgattaata atgttgtatt agttggacgt ctgacgaaag
    27721 atccagattt gcgttacaca gcaagtggta cggcagtcgc aacatttact ttggctgtga
    27781 atcgtaattt tacaaaccaa agcggcaatc gcgaggctga ttttatcaac tgtgtgattt
    27841 ggcgcaaatc agcagaaact ttagcaaact atgctcgtaa aggaacactt ttaggtgtta
    27901 ctggacgtat ccaaactcgt tcttatgata atcagcaagg acaacgtgta tacgtgacag
    27961 aagtagtagc tgacaacttc caattgctgg aaagtcgttc tgcatctgaa aatcgccaac
    28021 aaagcggcgg attccaaagt tctggtcaat cagctggcgg atttggcgga aacaataact
    28081 ctaatcaaac atctcaatca tccaacggta tgcctgattt cgaccgtgat acttctgacc
    28141 cattcggctc aagttcaacg atcgacattt cagacgatga tttaccattc taaacgaatt
    28201 tgataggagg gaaatagaat ggcacaacaa agaagaggcg gacgtaaacg tcgtaaagta
    28261 gactacattg ctgctaacca tattgaatat atcgattata aagatatcga attactaaaa
    28321 agatttattt cagaacgcgg taaaatcttg ccacgtcgtg taactggtac aggcgctaaa
    28381 aaccaacgta aaattactgt tgcaatcaaa cgcgctcgta tcatgggctt gctacctttc
    28441 gttagcgaag actaataaat cctaggaatt gaatgactga tccttttgat cagctattca
    28501 attccttttt ttctgtaaaa ataacctcta ttcgtttagt cgctcgaatg acgaaagtta
    28561 tggtaaaatc aaattagtca tatatgggag gaaaattatg gaaaataaaa acaatcattt
    28621 atccccgaaa cctctcttcc ttatcctgtt tatattaatc gaagtattgg tagtcttaat
    28681 tttaccattt cgttttgtta gcgcttcggt cattctattg atgaatttat ggggtgttcg
    28741 ctattggttg aaaatgaaac gttggcttat tttaaataaa cgtgaaaaaa tccagcaggc
    28801 ttcgactgta gctgaaacca atctagctta tgtctcagaa gtcatgcctg taggagtgat
    28861 tgcttatcag ccatcaagcc aaaaaatcga atggctcaat ccattagcat tgaaaatcaa
    28921 agatcaaagt ggattaacag aggaagaatt gctagatact ttttttactg caaaagaaaa
    28981 aaagaaaaac caagtctatc tagcagataa aacatttcag tttgatatgg atacagtaaa
    29041 gggtgttgtt tattttaatg atattaccca aaaccagttg ttaaaactcc atcagacgaa
    29101 cagtcagccg gtgattggta ttttatctat tgataactat gcagatgcca ttgataaatt
    29161 ggatgacaaa gagatttctt atttgaactc tttcgtcact acactagttt ctgactggat
    29221 gaatgaatac catgtctttt tcaaaagaat caacgcagaa cggtttttct tcgtagcaca
    29281 gatgatcgat ctgaaaaaga tgatggataa aaaatttgat ttgctggatc gctttcgagc
    29341 agaagctgaa aaacagagct cgcctatcac ccttagtatg gggattgctt atggctcaag
    29401 aaccctcgat gaaatcggtg gagaagctca gaataattta gatgttgctt tagtccgtgg
    29461 aggcgaccaa gttgtcgtga aagaggtaaa agaaggagca aaacctgttt attatggggg
    29521 ggaatctgct aagaatgtca aacgcacgcg tgttcgttct cgagcgatga gtaccgcttt
    29581 gcgtcggatt tttgatgaaa caggcgatgt gtttatcatg gggcacaagt ttccggatat
    29641 ggatgcttta ggctctgctt ttggtgtcag ttgtctggca cgcttcaacc agaaaaaagc
    29701 ttacatcgtt atcaacgaga acgagatcat tccagatgta gaacggtgtc ttgaagaaat
    29761 ccataagcat cctgaattag aaatgcaact aatctctcca gaacgtgcca tagagctgaa
    29821 aaaagaagag gacctcctcg tgatggtgga ttaccataaa ccttctctat cgatctctca
    29881 gggcctatat gagcaatttg ataagattgt gatcattgat caccatcgtc gtggggaaga
    29941 gtttccaagt aaacctctgc tgacttatat cgaatcttca gcttcttcag cttcagagct
    30001 agtttctgag ctgatccaat accaaagcag caaaacaaat cgcttagatc gttttgaggc
    30061 cacattattg cttgcaggga tggttgtaga tacaaatagt ttcaatgtcc gtacaactgc
    30121 acgaactttt gacgttgcca gctatttaag gtcttgtgga gcagacgcat ccttagtaca
    30181 atatttattg agctcggatt tagcgacata cttggagatc agcaatttga ttgctggaag
    30241 cgactatgtg acttctgata ttgttgtggc ggcagggaca gacgaaaaag agtataatag
    30301 tgttatagca gcaaaaacag cagatacatt gctttcgatg atcaatatca atgcagcttt
    30361 cgtcattaca aagcggacag acggcttgat tgggatcagt gccagaagta ccggcacgat
    30421 caacgtccaa atcatcatgg aatccttagg tggcggagga cacttcacaa acgcagctac
    30481 tcaactggaa aatacgacag ttgaggaaac aaaacagaag ctatttgctg caatcaaaga
    30541 aaatatgaat caaatatatg ataaggagta atccaaatga aagtaatttt tttacaagat
    30601 gtaaaaggaa aaggtaaaaa aggggacatc aaagaagttc cagcaggtta tgcacaaaat
    30661 tatctaatca aaaataactt ggcaaaagaa gcaacgaaaa gcagtatcag tcagttagca
    30721 ggacaaaaga aagcacaaga aaaacacgaa gctgaaatct tggaagaagc taaaaagtta
    30781 aaagaattct tggaaaaaga agatactgta gtcgaattga aggcgaaagc aggggaagac
    30841 agccgtttgt ttggttcgat cccttcaaaa caaattgctg aagggttaaa caaacaatac
    30901 aaaatcaaat tagataaacg aaaaatcgaa ttagaaaatc cgatccgttc actaggctac
    30961 acaaacgtac ctgttaagct tcatcatgag gtgacggcta cgatcaaagt tcatgtcgta
    31021 caagaataac aaatcttgat agcgatgttc agctggtaag tataaccaga aggaaaaaag
    31081 acaaaccgaa atttatctga gaaaacagct gcagcaatga aagcaaaagc tgcaaatcga
    31141 tttcagattg atttcggttt cttttttggg aacagcaaaa tagcataaag aaaacgagta
    31201 taataaaaga gaactatttt tttattacac tttcggtacg aatgatgact tttctctcat
    31261 tttcttgtaa aatgaagcaa ttgaagacaa ggagcgatac gtatgaatga agtgtggcag
    31321 gatcgaatcc caccgcaaaa tattgaagct gaacaagcgg ttttaggctc tgtttttctt
    31381 gacgcagaaa cgatcattga tgccatggag tacatagagc caaaagactt ttatcgtcgt
    31441 ggccatcaaa ttatttttga gacaatgatc gagttaaatg atcgtaatga agcgattgac
    31501 gtggttactg tcaaagaccg gctggaacaa gccaatttgt tagaagatgc cggtggttta
    31561 agttatctct cagacttggc attagcagtt ccgacagcag caaatatcgt ctactatgcg
    31621 aaaatcgttg aagaaaaatc attgctgcgt acattgatcc agacagcaac aggaatcgtt
    31681 acgaaaggat tcgagcaagg agaagaagtt caaacaattt tagatgatgc cgaacgcagt
    31741 atcttggagg tttcggaaaa aagaaatcga agcggctttt tatctattgc tgaagtactg
    31801 aactccacga ttgaaaatat cgatcgacta gctcaaaaca atgaagaaat cacaggtcta
    31861 ccaactggct atcaaggtct ggataaaatg acagcaggtt tccagcccga agaactaatc
    31921 attcttgctg cacgtccagc ggtaggtaaa acagcgtttg cattgaatat tgctcaaaat
    31981 gtaggaacaa aaacagatcg ttctgttgcg atatttagtt tggaaatggg ggcagaatct
    32041 cttgtaaatc ggatgctttg tgcagaaggt tcgattgagg ccagccattt acgtacaggt
    32101 cagctttcgg aagaagaatg gtcgaatctg atcgttgcga tggggagtct ttctcgagca
    32161 aatattttta tcgatgatac accagggatc aaaatctctg aaatccgtgc gaaatgtcgg
    32221 aaactagcgc aagaaaaagg caatctaggc ttgatattga tcgattattt gcagttgatt
    32281 gaaggtaacg gtaaagagaa ccgtcaacaa gaagtatcag atatctctcg ccaactgaaa
    32341 aaactggcga aagagctgaa agtacctgtc attgctcttt cacagttatc tcgtggagta
    32401 gaacagcgac aagacaaacg accagtgcta agtgatattc gtgaatccgg atcaattgaa
    32461 caagatgcgg acatcgtagc cttcttgtat cgagatgatt attatgaaag agaaggcgga
    32521 gatgacgatg atggaaacga gccaaaaaat gacaacgtca tcgaagtcat catcgagaag
    32581 aaccgaagcg gtgcacgagg aacagttgaa ctactgttta tcaaagaata caataagttt
    32641 tcgtcgcttt cgccaagaga agaattttaa agaagaggtt gtgagccttg agcaatcccg
    32701 agagctctcg taagaagatg caaggagtaa ccgcaggaac gactagagta cttaatttaa
    32761 acagatctcc aaagggattg tgacaaaagt cttgtcacag tccctttttc cgaataaacg
    32821 gtgttcaatc agctgaccct tggtattagc tcagcaaacg tcaaaatatg aggaactatt
    32881 ttgacgtttg tttctctaat actcaggtca aaacgctgat cttacgacct cttttcctta
    32941 aacggtgttc tcctgccaac tcctcggtca caagtcacaa tatcatcaaa catgagaagc
    33001 agttttttga aattgctcct tgtgactcag agttaaacgg caggctcaca acctctttct
    33061 taaacggtgt tcaaaagctt gcatctgcgg taatacgccc aatcaaacga aaaatatgag
    33121 aagctatttt tgtttggctg ttcttattac ttgatgcagg acagcttttt cacaacctct
    33181 gattcacggt gttccaaaag tagcttcttc gaaaaataag tcgaaatttc acaaaaattt
    33241 gagaagcaat tttcgtacat tccttcttat ttcttgaagc tgaccacttc tgttacaacc
    33301 tcttttttaa taaccattct aaaaagataa agttcgtttt ttaagcgttt ttataaaata
    33361 aaaataaaaa tcattcgatt ttatttgaaa tatgtatcta ttattgatac aatggttcag
    33421 gattaaataa tatagaacga ggtgttcaca tgtcatctgt agtagtagta ggtacgcaat
    33481 ggggcgatga agggaaagga aaaatcacag actttctcag tgaaaatgct gaagtgattg
    33541 cacggtacca aggtggcgac aatgcagggc acacaattaa gtttaacggt gtgacttata
    33601 aactgcattt gattccttct gggatttttt ataaagacaa gatcagtgtg atcggaaacg
    33661 gcgttgtagt caatcctaaa tcattggtta aagaattagc ttatttacat gaaaaaggtg
    33721 tagaaacaag taatttgcgt atttctgacc gcgcgcacgt aatcttgcct tatcatatca
    33781 aattggacca attacaagaa gatgctaagg gcgaaaataa gattggtaca acgattaaag
    33841 gaatcggacc tgcctatatg gataaagcag ctcgtgtagg tatccgtatt gcagacttat
    33901 tagataaaga aatttttgaa gaacgtcttc gtttgaatct agatgacaaa aaccgtcaat
    33961 ttgtaaaaat gtttgattca gaaccacttg attttgacga tatttttgaa gaatattatg
    34021 aatacggaca acaaatcaaa caatacgtca cagatacttc cgttatcttg aatgatgctt
    34081 tggatgcagg caaacgcgtg ttgtttgaag gcgcacaagg tgtaatgctg gatatcgacc
    34141 agggaactta tccttttgtt acttcttcta acccagtagc cggtggggta acaatcggaa
    34201 gcggtgtagg gccttcaaaa atcaataaag ttgtcggcgt ctgcaaagcc tatacgtcac
    34261 gtgtcggtga tggaccattt ccaactgaat tatttgatga aacaggagaa caaatccgcg
    34321 aagtaggacg agaatacggt acgacaactg gtcgtccgcg tcgtgtaggc tggtttgatt
    34381 ctgtagttat gcgccattct aaacgagttt caggtattac caacctatct ttaaactcga
    34441 tcgatgtatt aagtggattg gaaacagtaa aaatttgtac ggcttatgaa ttagatggcg
    34501 aactaatcta ccactatcca gcaagcttga aagagttgaa ccgctgcaaa ccaatctatg
    34561 aagaattgcc aggctggtca gaagacatta caggatgcaa aacacttgca gaactgccag
    34621 aaaacgcacg taactatgta cgtcgtatct cagagcttgt tggtgtacgt atctcaacat
    34681 tttctgtcgg acctgataga acacaaacaa acatcttaga aagcgtctgg gcgcaaatct
    34741 aaggataaaa acgacgactg aatcaggacg ttcttaccag agcgagtcga agtgtttatc
    34801 tatacgataa taataacttg aagaggtagt gaaagagacg tttagcctta ttgccgatag
    34861 gctgtctctt gaacactgtt tattcagaat aagggactgt gacaaaaac
//
LOCUS       DAZABD010000026        34627 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000026 DAZABD010000000
VERSION     DAZABD010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34627
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.026"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(513..1654)
                     /locus_tag="ACHALG_001310"
     CDS             complement(join(513..1426,1426..1654))
                     /locus_tag="ACHALG_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFN2586732.1"
                     /translation="MAKAKKKFDEDFKKMILDLNQSGQSVEELAAQYGIATQTIYRWK
                     KLHTKNVATGMTEAEILAMKKEMARMQEENTIPKKGFNHIRSKVKMSDVFEFIQSNVH
                     DHDVKQMCTVLEAPKSSYYDWKKKKPSKRQSENKRLNRLISGIYFENKGIYGAPKIHK
                     ILVGRGETLSLKKVQILMRKLGLRSVTLKKYKPGKQAKVKSEGRKNLLNQDFTTTSIN
                     QKWVTDITYIHTLKDGWTYLSTIQDLHTKKVIGWKFGKQMTKELVIETLDNALLNQTP
                     SENLIIHSDLGSQYTSEAYEAKLNELNIRHSFSRKGCPYDNAGIESFHASLKKEEVYP
                     SKAYENFEAAYLALFQYIEGFYNRKRIHSSINYLTPNQMEELALQA"
     gene            complement(1722..1937)
                     /locus_tag="ACHALG_001311"
                     /pseudo
     CDS             complement(1722..1937)
                     /locus_tag="ACHALG_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
     gene            complement(1937..2167)
                     /locus_tag="ACHALG_001312"
     CDS             complement(1937..2167)
                     /locus_tag="ACHALG_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFN2586733.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            2463..2801
                     /locus_tag="ACHALG_001313"
     CDS             2463..2801
                     /locus_tag="ACHALG_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2586734.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPIQIET
                     LVQRISTREDFAIVECEIVAIFVEEGILHDPTHINVDKWQPLIYKLREYTTANQSLSL
                     NFRFQEFKES"
     gene            complement(3006..3581)
                     /locus_tag="ACHALG_001314"
     CDS             complement(3006..3581)
                     /locus_tag="ACHALG_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HFN2586735.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPEQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGF
                     YEWNEEKDKFMFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRMPLVL
                     TEEQIEPWVTDVSFARKIITQQMPELVMEKV"
     gene            complement(3946..4527)
                     /locus_tag="ACHALG_001315"
     CDS             complement(3946..4527)
                     /locus_tag="ACHALG_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN2586736.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASETGKGQFYYYFS
                     SKKEVCLAVIHAHMVVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWIKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(4710..5336)
                     /locus_tag="ACHALG_001316"
     CDS             complement(4710..5336)
                     /locus_tag="ACHALG_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFN2586737.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFSLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(5358..6686)
                     /locus_tag="ACHALG_001317"
     CDS             complement(5358..6686)
                     /locus_tag="ACHALG_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HFN2586738.1"
                     /translation="MEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTCI
                     NIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARFL
                     SNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPEK
                     LVIIGAGYIGLEFASMFNEYGSKVVVLDAHPEFLPREDEDIAQMILEDMTNAGIEFHL
                     GVSVDQVADQESTAAVTFTENGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDRG
                     AIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVPY
                     SVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETDL
                     ILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(6845..7246)
                     /locus_tag="ACHALG_001318"
     CDS             complement(6845..7246)
                     /locus_tag="ACHALG_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFN2586739.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(7332..7538)
                     /locus_tag="ACHALG_001319"
     CDS             complement(7332..7538)
                     /locus_tag="ACHALG_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2586740.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(7633..9135)
                     /locus_tag="ACHALG_001320"
     CDS             complement(7633..9135)
                     /locus_tag="ACHALG_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFN2586741.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(9153..9608)
                     /gene="argR"
                     /locus_tag="ACHALG_001321"
     CDS             complement(9153..9608)
                     /gene="argR"
                     /locus_tag="ACHALG_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFN2586742.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(9706..10323)
                     /locus_tag="ACHALG_001322"
     CDS             complement(9706..10323)
                     /locus_tag="ACHALG_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFN2586743.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            10544..11917
                     /locus_tag="ACHALG_001323"
     CDS             10544..11917
                     /locus_tag="ACHALG_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFN2586744.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            11992..13104
                     /locus_tag="ACHALG_001324"
     CDS             11992..13104
                     /locus_tag="ACHALG_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFN2586745.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(13176..14813)
                     /locus_tag="ACHALG_001325"
     CDS             complement(13176..14813)
                     /locus_tag="ACHALG_001325"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFN2586746.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(14981..15589)
                     /locus_tag="ACHALG_001326"
     CDS             complement(14981..15589)
                     /locus_tag="ACHALG_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFN2586747.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            complement(15973..17124)
                     /gene="queG"
                     /locus_tag="ACHALG_001327"
     CDS             complement(15973..17124)
                     /gene="queG"
                     /locus_tag="ACHALG_001327"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFN2586748.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(17143..17895)
                     /locus_tag="ACHALG_001328"
     CDS             complement(17143..17895)
                     /locus_tag="ACHALG_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2586749.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            complement(18036..18986)
                     /locus_tag="ACHALG_001329"
     CDS             complement(18036..18986)
                     /locus_tag="ACHALG_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFN2586750.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            complement(18987..19826)
                     /locus_tag="ACHALG_001330"
     CDS             complement(18987..19826)
                     /locus_tag="ACHALG_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFN2586751.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(19823..20569)
                     /locus_tag="ACHALG_001331"
     CDS             complement(19823..20569)
                     /locus_tag="ACHALG_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFN2586752.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQPVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            20807..22177
                     /locus_tag="ACHALG_001332"
     CDS             20807..22177
                     /locus_tag="ACHALG_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFN2586753.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(22232..23128)
                     /locus_tag="ACHALG_001333"
     CDS             complement(22232..23128)
                     /locus_tag="ACHALG_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFN2586754.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(23242..23412)
                     /locus_tag="ACHALG_001334"
     CDS             complement(23242..23412)
                     /locus_tag="ACHALG_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2586755.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            complement(23409..24086)
                     /locus_tag="ACHALG_001335"
     CDS             complement(23409..24086)
                     /locus_tag="ACHALG_001335"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFN2586756.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(24177..25082)
                     /locus_tag="ACHALG_001336"
     CDS             complement(24177..25082)
                     /locus_tag="ACHALG_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HFN2586757.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            25257..26711
                     /locus_tag="ACHALG_001337"
     CDS             25257..26711
                     /locus_tag="ACHALG_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HFN2586758.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVVPSH"
     gene            26727..27758
                     /locus_tag="ACHALG_001338"
     CDS             26727..27758
                     /locus_tag="ACHALG_001338"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFN2586759.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            27765..28952
                     /locus_tag="ACHALG_001339"
     CDS             27765..28952
                     /locus_tag="ACHALG_001339"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFN2586760.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            29902..31764
                     /gene="ltrA"
                     /locus_tag="ACHALG_001340"
     CDS             29902..31764
                     /gene="ltrA"
                     /locus_tag="ACHALG_001340"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFN2586761.1"
                     /translation="MENVSASHSIRHMEYYGLIDSFDSLYKESSENKIFSNLMEIVTS
                     PNNILLAFRNVKGNSGSTSPGVDKKNIDDLKAIPNIEFIKTVQTKFSEYKPQPVKRVD
                     IPKPNGKTRPLGIPTIWDRIIQQCLLQVLEPIMEAKFHDKNYGFRPNRSAHHAFAQAV
                     RMAQLSKLTFVVDIDIEGFFDNVNHSKLIKQFWTLGVRDKWLLGVIRAMLKAPIIHRD
                     GRIEHPKKGTPQGGVLSPLLANVVLNELDWWISSQWETHPTRKNYDCYHQTRKQWIKS
                     NKYRALRASKLKEIYIVRYADDFKIFCRKRSDADKIFLATKLWLKDRLKLDISAEKSK
                     VVNLKKQKSDFLGFTMKLVRKRKSFVIETHMCAKAMSAASNKLAKQIKVIQHSPTQVE
                     LQKQLSIYNSMVVGIHQYYKKATHISVDCPRINQRLMIIIKNRLDIKRKGPPPSDFIK
                     KNYARSKQLRWLNGVPMLPFGFIKHSHTSLPKIGTCKYTPEGRVLIHRYLTFPIDIIN
                     QLMNMSNPNESIEFRDNRVSKYSSQVGRCRVTKEVLELADIYCHRIVPKNLGGKDNYQ
                     NLVIVHRNIHSLIHEEDKTRASLLIQNMRVTSAQLEIINQFRKKAKNRELSLNL"
     gene            31878..33176
                     /locus_tag="ACHALG_001341"
                     /pseudo
     CDS             31878..33176
                     /locus_tag="ACHALG_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
     gene            33148..33534
                     /locus_tag="ACHALG_001342"
     CDS             33148..33534
                     /locus_tag="ACHALG_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2586762.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(33734..34402)
                     /locus_tag="ACHALG_001343"
     CDS             complement(33734..34402)
                     /locus_tag="ACHALG_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFN2586763.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
BASE COUNT         9900 a         7003 c         6371 g        11353 t
ORIGIN      
        1 atatattttg aaactcttta aaaataaaat ctaaacgttc taacaaagct tgatatacat
       61 ttttatttaa atattttcta tccacattat cccttcttca aaattttact aaaaaatcca
      121 ataattaaaa atacgaaatt tataaataca aaattcatct gcttattata ttaaaaacac
      181 attatttttt taattcaaaa atacaaaaaa ttgttactac atagaatcag atttaagcta
      241 acattttatt acaatataaa gaatatctca agttgctaca aaactagaat cttcacttgt
      301 tggataattt gctataatca ctgacattat tgcttttgga tttgagtcaa tacctcggac
      361 acgatttgtg agaatttttt cgagattcca gcgggtgagt atctcatcgt ttcgaccagc
      421 ggtcaaacca aaaccgtttg accgctgatc gaagcgatga agttgaaacc ctgtggaaat
      481 ctcgccaaaa aaatgtaagt ggtagctagg aactaagctt gcaatgccaa ctcctccatc
      541 tgatttggtg ttaaatagtt gatactacta tggattcgct tgcgattgta aaatccttca
      601 atgtattgaa ataacgctaa ataagcagct tcaaagtttt catacgcttt tgatggatag
      661 acttcttctt ttttgagtga ggcgtgaaac gattcgatac cggcattatc gtagggacag
      721 cccttacgac tgaaggagtg ccgaatgttc aattcattca gctttgcctc ataggcttcg
      781 ctagtgtact gagaacccaa atctgagtgg atgatcaagt tctcggaggg tgtttggttt
      841 aagagcgcat tgtcaagggt ttcgataacc aattctttcg tcatctgctt gccaaacttc
      901 caaccgatga ctttctttgt gtggaggtct tgaatagtgg agaggtaggt ccagccatct
      961 tttagggtat gaatataggt aatatctgtc acccattttt gattgatcga cgtagtggta
     1021 aaatcttggt ttaacaggtt tttacgtcct tcacttttaa ctttcgcttg tttgccaggc
     1081 ttgtacttct tgagggtgac tgaacgcaag cctaattttc tcatcagtat ttgtactttc
     1141 tttaaggaca aggtctcgcc acggccaaca agaatcttat gaattttagg cgcaccatag
     1201 atacccttgt tttcaaagta gattcctgaa atcaagcggt ttaagcgttt gttttctgat
     1261 tggcgcttgg aaggtttctt tttcttccag tcatagtagc tggatttcgg tgcttccaaa
     1321 acggtacaca tttgttttac gtcatgatca tgaacatttg attgaataaa ttcgaagaca
     1381 tcactcattt ttacttttga gcgaatatgg ttaaagcctt ttttaggata gtgttttcct
     1441 cctgcatgcg ggccatttcc tttttcatcg ctaggatttc tgcctcagtc atgccggttg
     1501 ctacattctt tgtatgaagc ttcttccaac gataaatcgt ttgtgtagcg ataccatatt
     1561 gcgctgccag ttcttcgacc gattggccag actggtttaa atctaagatc atcttcttaa
     1621 agtcttcatc aaattttttc ttggcttttg ccatcgtgga cacgtccttt tcttagtagt
     1681 tagattctac caaatcgtgt ccatgaaatc atcctaacat caatatctag ctcttcaggt
     1741 aatgagtaac gtgtcggtaa attctttata gtggaagtaa ttggacgtat gaccgaaaac
     1801 attgttttac ggttaaattc ataacgagag acgactagtc ctggtcgtct tttttgaatt
     1861 tcttttccta cggagggatc aaaatcaatc catacaatat cacctttttt aggaatataa
     1921 attctttctt ctgacactat aaaatttctc tcccttcagg agttaaatct tcccattcgt
     1981 ctttctcata atactcggcc tcttccccac cactaaatgg atcttcaatt tttggaacta
     2041 acgtaattgt tccatcatca gaatacacaa cgatatattc ttggttttct gaaggtttct
     2101 ttccgttatc agaaggtaaa gttaccacta cagaacttcc ttgtaatcta gattttgtaa
     2161 caagcattat catcactccc ttaataaaag tatactatat aacactaagt aacgcaaaat
     2221 tcccctttgt aaactatcta gaaaattgtt ttgtttaaag tatatcttat tggatataaa
     2281 tagaaataaa ttgtagccag ttcatttttt ctttaaataa aaaaataatc caagaaacat
     2341 tgaacctatg gatccaaaag ctacccaaat actatagcta actccccgct accttcaatc
     2401 ttgcagataa aacgctttgg aaaaaaatcg agaaaatcgc aaagacgaca ggccatccta
     2461 tcatgcctga ttataaacaa aaagctggct attccttttg ctctgacaaa tttcaattgg
     2521 gaaagttcac gaaactagca ggtgaagaag tttcaactgt tcggattaaa gaatgcccga
     2581 ttcaaataga aacgttggtt caacgaattt caactagaga agactttgct attgtagaat
     2641 gtgagatcgt agctattttt gtagaagaag ggattttaca tgatccaact catatcaatg
     2701 tggataaatg gcaacctctt atctataaac tcagagaata tacaactgcc aaccagtcgt
     2761 taagtttgaa ttttagattc caagaattca aagagtcata atgataaaca tcattaggta
     2821 taaataaaaa agactcgcag aacgagtaaa aaacaaatag aagctatacc ttagaaaggt
     2881 cgactgtgac tctatagatg cagtatatgc agcttatgag catttcttct gttctctttg
     2941 tttttgggat ctgtgctgaa gtctttttcg ttttttgccg ctctttcttt gaaaaatcga
     3001 ttatgttata ccttctccat tactaattca ggcatctgtt gagtaatgat ttttcgcgca
     3061 aaagaaacat ctgttaccca aggttcgatt tgttcttcag tcaagacaag cggcattcgg
     3121 tcatggattg gtgcgactgt gcggttaggt tcagtagtca ttatgattga ttctgccatt
     3181 ttttcatcct gatggctacg ataaaaaccg ccaatataca ctattttttc cttatttgaa
     3241 aacataaatt tgtctttttc ttcattccac tcataaaagc cactcattgg gaaaacacaa
     3301 cgattttgca aaaatggttg atgaaaggtc tttttttctt ctactgtctc tgcacgggca
     3361 ttaatcatca attgtccttt tttaaatccg gtaaatcccc acttcatgat ttccggagtg
     3421 aggatgtttt cttcctcttt tcttaacgtc acaacaagat ttgacggaaa aacttctcca
     3481 gctgctaatt ctgctgcctg ctccggttgt ttcgtctggg cttctcgata ataattctga
     3541 aggagctctg actctagatc aaaaaaataa cgaccacaca tcctaatccc tcccaatctt
     3601 tttttctatc atacactgca acaagaagtc cgtaccaaca aaacgctttc aatttgtgta
     3661 gaactaaaaa agttgtgaca agtgacaaac tctccagtga atggtattct cctgccaact
     3721 cctcggtcac aagtcacaat attcaacaaa catgagaagc tgctttttga aattgttcct
     3781 tgtgactcgg agtgaaacgg caggcttacg acctctaggt aaacggtgtt ccagaagtag
     3841 cttcttcgaa aaataagccg aaatttcaca aaaatttgag aagcaatttt cgtaaattcc
     3901 ttcttatttc ttgaagctga ccacttctgt cacaacctct aattattaaa gaaccttttg
     3961 cgaagttaac aatctttctt tgatcaattg aaaattttgt ctaagtagtg atagatcctg
     4021 tgtgactttc aacaataaga tacttccctg gatttcagca atcaacgctt tggcttgacg
     4081 gtctgcttct tcatcggaca gtttttcttc agaaagtgcc aggatatttt gtttcgtata
     4141 cttgatccat tgattgacta attctagtag caacagacgg tattcttcat ccactgtact
     4201 caactcaata atcaaattac ctacaggaca gccatgataa gtgatctgtg tctcatgatc
     4261 agcaaaaatc cagtccaaca tttcttctaa tgcttcttct ggctgttttc cagcatttaa
     4321 gatgtcttcg aacaattctg tctgccacac gaccatatgc gcatggatca ccgctagaca
     4381 aacttctttt tttgaagaaa agtaataata aaattgccct ttccccgtct cagaagctgc
     4441 tagaatatct cggattgacg tttcatgata gcctttttta taaataagat ctttggtcgc
     4501 ttccaagatc ttttcctttg ttttcaatga tattctcctt tttcatttct catagtccta
     4561 tattatacaa taaattcatc attttttctt cttaatcgtt taaaaaagac tgtgccaaat
     4621 tatctgacac agccctcact ataaaactgt tctcatatag ctagatgttc attgattcaa
     4681 gaactgcgag gacaaaagca attcctatct tatgatagtt gctctaatac ttcctctgtt
     4741 gtcatcactt gatctgcaat aaagccaaag ttcgtcaacg ctgcatccat tgcttcttga
     4801 ccaggagcac caattgcatc tgtcgctacc acaacattga atccactctc taccaattca
     4861 cgcatatggg aatcgctaca caagtttccg ttcattccac caagaatcac tgtgtcgatt
     4921 ccattttttc tcaattggag cgccaaatca ttgctttctg gtccaaagat tttatgagga
     4981 gagctgataa tcgtattttc atccaaatat ggtttcaatt cttcgatgaa atcagctcct
     5041 gaaccttctt caactgattc atattgactt ttcctagcaa acatctgttg ctcaagcatc
     5101 atatgttctc ctgcaccgcc aaattgccat ttatggtcat gtgggtaaaa ataatgagga
     5161 gaaataaaga gtttgtatcc tttttctttt gcttgtttca ccatttcgat catgttttcg
     5221 atcgtattgt atttttctaa gatatctttt gtcaaactaa agccaccgcc tgctggtttc
     5281 aagaactcga cttgcgggtc agtgagaact actgctgtat ttttattatt gaacatggtg
     5341 gattctccat tcgttattta ttttaataca tcatttaaag cttcactcat tgttggatga
     5401 gaatagattt gatctctcaa taacgtataa ggaagttttc cattcatagc tagcgaaatc
     5461 aagttgatga cttcatgaga atcttccgca tagatacttg cccctagaat caagtcagtt
     5521 tcagggtcga ctaaaacttt gaatacacct ttaggatctt ctaatacttg tgctttaggg
     5581 acaccgcttg tcatatactt gaataatttg taattgacgc cttgtgcttt ggcttgtttt
     5641 tctgttaaac ctacattgga taaagtcgga gtgataaata cactgtaagg cacagctttc
     5701 cggttattcg ttgttcgttt attctcgccc ttcaactgat ccataatgat tcgataatca
     5761 tctagagaga tataagtgaa ctgcaatccg cctttgacgt ctccaatcgc ccaaatgttg
     5821 tctgcatctg tacgaaggta ttcatccaca gcgattgctc cgcgatctgt tagtttaaca
     5881 tctgtatttt ctaatccaag tgatgctgta cttggttttc ttccagttgc agccaaaact
     5941 tttgacgctt ggattgttac ttcttggcca ttttctgtaa aggtgacggc agctgttgat
     6001 tcttggtctg ctacttgatc tacagataca cctagatgga attcgattcc cgcatttgtc
     6061 atgtcttcta aaatcatttg cgcgatatct tcatcttcac gaggaagaaa ttcaggatgg
     6121 gcatcaagaa cgacaacttt agatccgtat tcattgaaca ttgatgcaaa ttccaagcca
     6181 atatatccag ccccgatgat cactaatttt tccggtaatt gttcctgatc catcgcttgc
     6241 gtagaatcaa tcaagtagcg acttgtattc aatcctttga ttggtaaaat gattggttca
     6301 gaaccagtat tgatgaaaat ccgttcgcct tcaacaatca ctttttcacc attattcgtc
     6361 acttcgattt gatggttaga aagaaaacgt gctgtaccgt ccatcactgt cccagttgcc
     6421 tcatcgctga tcatatgata atttttggcg cgaagcatgc ccgtcaattt ttctttccga
     6481 gcaacagcct ctgtaaagtc gattcctctt tctccattga aaatcaatga tttagaagga
     6541 atacagccga tgttgataca tgttccgcca tacatacgtg ttgacttttc aacgactaaa
     6601 accgattcgc cttttgaagc taacgccttc gctaaagtct taccgccctt tccaaaaccg
     6661 atcacaatat tttgatattt ttccatcctt tttccctcgc ttcttttttt acctaccgat
     6721 tggtacaaaa atatgataac cgataattgt ctattctcca aatcatctgc tttatttgtc
     6781 aaaaaaaacc agacaaaaag cggtcctttc acgtttttag tctggttcga tttcctttat
     6841 tcttctaaac aatcgttact tctacatcga tatttcctct agttgctttg gagtaagggc
     6901 agaattcatg cgcatcttct gcaatttttt gggcttcttc ttcgctgatg ccatcgatat
     6961 gcacttctaa tactacaccg atctgatagc cgccatcatc taaattataa agagagacac
     7021 gtgcttttac tgtggactct gctgtcattc ctgcttcttt gatcatatgt tgtaccgccc
     7081 cattgaaaca agaagcataa cctgctgcaa ataattgttc tggatttgtt ccttctgcat
     7141 ggattcctgg aggagtgatt ttcatatgca tcgagccatt tggtgcctcg acttctccat
     7201 tgcgcccgcc atggttgatc attgctgttt cgtatgcttt tttcattgta ctgctccttt
     7261 cgattgacca tttgctactt ggctataatt atagtgtatg acaagtaagg cgctttctac
     7321 aaaaattctg cttattcgaa taccagaatg ccgtcttcta ctatttttcc aaaaaacaaa
     7381 aaaatactac tcgtgaacaa cagcgtcata tttgttccaa aataaatagc aatcttttct
     7441 acataatctt ttatatgatt gattttaaga tacatatata ccagaaagtt gaccccataa
     7501 accaatactg ttaaaaaaat aaccatattc gttaccattt tttcctcctt ttgctggtct
     7561 ccctaccagc actcgattta accataccac tttttttgcc gtccaagcaa ctagcctttc
     7621 aaaaagactg gtttattctg ctgttaatga ttcaagatca gaaaaatgtc cgaataattt
     7681 tgctaatttt actaacgcca acttgttctt tccgaattca tcattttcta tctgtaatac
     7741 tagtaacggc tcatgtaagc ttaaacggat caaaaaccag ccgtttccgt atggctcttc
     7801 tagatcatat cgaatacctt cttcattttc cgggtcgaag ttcatccctt tttcttcttc
     7861 caacagtcgc ttcatttctt ctaatatctt gtttccttgt tcgcgtggtt catcgctgaa
     7921 caattttaat cggatttctt gtacttctac gggctgtttc aactctttga tttgttcatc
     7981 taatgtgata ccttttcttt ttaattctgg caaaagcatc aggatctttg cgatgacata
     8041 tgcaccatca tccagaaaat agttttcctt gaatgcagcg tgtccgcttg tttcgatggc
     8101 tagctgtgta tctttcccaa gacggttaag ttctaatgct ttattgatta catttcgata
     8161 gccggaaata taacgaactt ggatgcctcc tttttcttca atgaatcttt gtagatgaga
     8221 agaagtaggg gaattcgtca cgattcttgt tcctggatgc tcttttaata cgattgtact
     8281 taatactgcg atcaaattat ttcgattgat cactgttcca gaagcactga caactgcaga
     8341 acgatccaca tctgtatcga aaatgatccc aagatctgct tcatttgcta aaacagcctg
     8401 cttgatactt tccatcgctt ctttattatc aggatttggg atatgattag gaaatcggcc
     8461 atctggttcc aaaaattggg aacctgttga atccgcacct aagctattca atactttttc
     8521 agcaaagaat ccaccagcac catttccggc atcaacaatg attttccaac catttagtgg
     8581 tttgctttct agtgttccaa tccccttttt aatttttgtg actaaatctt gcgcataagg
     8641 tgtcaaaagg tctgcttttt ctacgcttcc agtttgttcg ctttttattt tttgctgctg
     8701 tgataggata aaagcaatat cttctttttc tgctcctcct tctgaggtaa agaattttat
     8761 tccattataa taatagggaa gatggctagc agtcagcata accgcagcat cgcacgagaa
     8821 ctgttcaaat tgagtggaca taaacatagc aggcgtagta gctaattcga aatcgatgac
     8881 atgtatccct tgttctttca gactttggat caacgcctct ttcagtgctg gtccgcttat
     8941 cctactgtca tgaccaatcc caatcgtcca ttttttattt ggatcggttg atttcttctt
     9001 tttctttatc cagtttgcta gtccccagcc gatctttttt accatgtctg tcgtcaagtt
     9061 tacttcatat ccttcaactg gtaatgcgat tcctcgaata tccgatccat tttgcagcgc
     9121 tttcatatct ttcatataaa agtcctcctc atttattccc tcattttttc cagcttttct
     9181 gcaaatcgct gtgcttcttc ttctgagcgg caaataataa tgaatgtatc tacaccggcc
     9241 atcgttccga caacttcagg gtaagccact tcatctagcc aattcgttac cacatcggca
     9301 ccatttcgtt cagtcaatac gataaccatg aactgaacag actggatacg caaaacctcg
     9361 cgtctaattg ctgtacgcaa acgttcttca tttaaagaaa cagatggctg gttaaataat
     9421 gtatagcgta ctattttgtt ttcattttga ctttttacta acttcatttc tcgaatgtct
     9481 cgagaaatcg ttgcttgagt cacatcggcg cctgcttcat gcagccgcgc aagcagctct
     9541 tcctgtgtcc caataggttg ttctgtaatg atctggcgga tcagtgcttg tcgctgtttc
     9601 tttttcatat tagacacctc tcaatgttga ttatagcaaa agataagtaa tatttcttca
     9661 tattttggta ttttttactt ttattttttg ttttcattca ggttgctaac ctttttctat
     9721 tttaacacgc aaaatcgtct tcattttttc aggtgctgat tttcttggat cgctgatgta
     9781 aatctctttg tgttctttag aagctctttc atagccttct tttgcgatga atgcttctaa
     9841 tttctcaaac gttgctggtt cttcggcata gggcccgacg tgtaagattt gggcacaaag
     9901 accttcttcc atctttttga atactacttg ttctttcaag tcttccggaa gtttgctaga
     9961 acgctgaatg gcttcttgtg ctatcgcttc tgtaacaaag tcaggctgtc ggatcatcaa
    10021 ttgataagaa aaatgttctt ttttcatcac tggttcatgc aaatactttt cttgcagtcc
    10081 ccattgtcct tccagtggat agacagtata catctgataa tcaggaatca gccaattttt
    10141 tttaggcgac atgcgtattg catagctgac tgcatataga caggctactc ttcgtttaaa
    10201 gtcttcctga ttcgggtcgc cgatccctct gatgctaaag aatgattttt taggaacctc
    10261 tagtatttgc gggctttttt tcatactata gctcttgtct ttacgccatt caattttttc
    10321 catttttctt cctccgcgaa attccattta gttatttatt atcatacctc tttttgagtg
    10381 acttggctaa tcgtgttgac actatttttc agtttgtgct agaataagtg aagtaaataa
    10441 atcgtacaga taacacgttt cggcgtgtcg agagagaggc ggtttctatt tttataatag
    10501 aaatcactct ctttttttac gaaatttttt agaggtgatg taaatgacaa aacaaaaaca
    10561 gctgctttta gtacctgcag taattgcaac agggatgatg tcttttgcgg gcgtattgat
    10621 cgagacggcc atgaatgtaa ctttcccaac tcttatcaga cagtttgatt taacgactgc
    10681 acaagtccaa tgggtaacaa cgatttatct tttgatgatt tcgatcattg ttccgctgtc
    10741 tacttatttg aatcgcaatt ttagtctcaa aaaactcttt ttagcttcaa atctgctttt
    10801 cttggcaggt ataatgatcg atttcttctc tcctaccttc atggtcctat tgtttggtcg
    10861 attgctccaa ggtgcagcga caggtatcgc tcttccgttg atgttccata ttatcttgac
    10921 atttgccccc ctcaatcgac gaggcgcgat gatgggtgtc ggtactttga caacggccat
    10981 tgctccagca attggcccca cttatggagg cattatgact tcacatttta catggaatca
    11041 tatcttcctg tttcttgtgc ccgttttgat tctttctttg attatcggct ggtttgctat
    11101 tcccgagatg gcagtacaaa aaagtggtta tttggattgg ataagcgttt taggaatttg
    11161 tcttttgttt agtggaagtc taacattttt gagtacgttg ggaacagttt ccagctggat
    11221 tgctttaatc atcggcggta ttggctggtt ccttttttac tatcgaagta aaaaagtgga
    11281 gcatccactg atccgactgt ctattctttc aaataaaaca ttccgcttgt ttttgattgg
    11341 cttcttagtt tatcagtttt tattacttgg tgtatctttt gtcttgccga atttccttca
    11401 gatcgttcaa ggaagtagtc cttttgtcgc tggattagcg atgcttcccg gcgcagcggt
    11461 tggtgctttg ctttctcctt tttccgggaa aatgctggat caatatggtc cgaagaagcc
    11521 aatttttgct gggctgattc tttctttgat tggttggcta gcattattcc tactcatagg
    11581 acacgcttca gtgggtgtat tgattgcttg tcatgtgttt tatatgatcg gtatcggatt
    11641 atcttacagc aatatgatga ccaccggaat gaatgcactg aatgaagaac tacaaggaga
    11701 cgggaacgca atgttcaata ccttgcagca gttttctggc gctgtggcta cttcattagt
    11761 agcggtcatt atcaattacg tccagcatca tacttcccat aactatgaag tgtctactac
    11821 attaggatca aaagcagcat taggcgtctt gttgctgttg cttctcgtaa gctttgctcg
    11881 attcgcctat tatctgttct ttgctaaaaa agcgtagatt ccatatacgg caggtgttgc
    11941 atctgccgtt tttttgtgtg aaaatggttg tttgtaaaag gaggcttttt tatggaaaaa
    12001 attgccatta tcggtggcgg gatcattggt atgaccctag ccagttactt agatacatca
    12061 aaattcaatg ttgctttatt tgatgaaggg acaggacagg caactaaagc tagcgctggg
    12121 attatctccc cttggttgtc gaaacgcaga aacaaacaat ggtatcgtct ggctaaagac
    12181 ggcgctgctt ttttccctaa acttataaaa gatttccaac ttgatcaaac gatttacgca
    12241 caatcaggga cacttttgct tcgacctgaa aaagatttag ctgaattggc tgttcttgcc
    12301 gagaaacgaa aagaagaagc gccagaaatt ggaaacatcg aatggctttc acctgaacag
    12361 acatctcaag cacttccgtt attgaaacca gcagtttcac tgcgtatcag tggtggtggt
    12421 cgtctggatg gaaaagcata tttagaacat cttggtcagc gtttagcaca aaaacaaatg
    12481 gagcagatcc aggcaaaagt cactttgcat aagaagagcg attcttggct catcaaggga
    12541 ccgtttggag aatacacagc tgatcgtgta attttgacac ctggcccggc acttaagcct
    12601 ttattacaag aattaggcta tgctgcagat atacgtccgc aaaaagggca gctcttagca
    12661 tttcagacat cttataagat gagtcaagac tggcctgttg ctatgcttga cggagaagct
    12721 gatttcattc ctttcatgga tggaaagatc cttttaggtg ctacccatga aaacgatcaa
    12781 ggctgggatt tggctcctac tagcgaagct tataagggat tgactaagag tacagaacag
    12841 ttcttgactg atccagatca gataaaaaac tggccatacg agttccgtgt aggtacccgt
    12901 gcttatacgt cagatttttc cccatttttc ggcgtactgc cagatgatcc aagcttactg
    12961 gctgctagcg gtctaggctc ttctggcttg accacaggtc cttatatcgg ctatctatta
    13021 gccaacttct taaatactgg tcattggcct acggaagatt atcaaaagga catctccacc
    13081 tatattacga aaaatccatc atagaaaaac agctttttca agtaaggatc ccgagcgata
    13141 ttgcgcatgg attacttctt gaaaaagctg ttattttagt tcaatttctg aatcaaattc
    13201 tccaaacttt cttttgcttc atctggcaag agatcttttc ctatctgagc ttcaatgaat
    13261 cgtaaacgaa tatcaagtcg ttgtttcacc agatttttat aggacgtatc ctcaaaagaa
    13321 acaggaaaat ctggtatagg taaatagtgg atgtcttcaa atggaccaaa cgacttgaat
    13381 tctgccgttt gttgcacgat tctttcaacg attgacaatg tcgtttcagg tttgattttt
    13441 ttatcatcga aatatccagt attcaggaca tagcaggctg tttgctgttt caaaaacaac
    13501 tctcgaaaag cttgatagtc ttccctaaga ggatagacac ggaaaggatt agcaaatggt
    13561 tcaatgacta gctcatctag ctgtcttttt gtagtgatat tttccgcgtc tgatcgtttt
    13621 gttgctaatg ttgcaccaaa agacgctgct aaaactgggt cattgatctt gataattggc
    13681 ggaagcgaat catctttcat gatccaaaaa acagcatcga tcttttcgct aagatgatcg
    13741 actcggtcgg gtgtcgcata acgtgatttg actgtccgcc catttccgtt tcgtatatct
    13801 cctgttacta ggactttttc tcctttactt gtacatgtca cgcccacatt ctgacaagta
    13861 aggaaagaag aaatggccgg atcatcagac ggatagtctt gtgttttatc aaagtaagaa
    13921 ggttccaagg cagtagcaaa accatttttt cgagaaataa cgaaagcatc gtcatgcaag
    13981 atcgaaacag agtgaccttc cgtatgtttt gctaaggtaa tcgttgattt tccagaaccg
    14041 gacaacccaa atacagccat cgtataggtc tggtcattac tagcatattg tttcatccca
    14101 ccgtggcagg caataaagcc attgcgattt cccaatgccc aagcaagcgt caaagtcgct
    14161 ttttttaatt ctccaaagta acgtaatcct agcacagccg ctgtattatg gagagcatca
    14221 aacaaaatga gtccatttgg aaatgtttca tgtgtccatt cgggatcaca aaagatataa
    14281 agatctccct catcaaagga aacagacttt tgataagtct ctaagatctt cgaattcgct
    14341 ggctgaaaat tcagcaaata agaatacaaa ttgttctcaa atccttctgg aatcagcaaa
    14401 tgactttgca cagaaaattc ttcagatagg ccaacgatta cttttgtatg gtaaaactgg
    14461 cgcttgcgac tttgatacac agcttctctt aaaattttct cgtatttgct ttgttccttg
    14521 tctaagtcct caaagatccg ccgagcactt gctgttcttc ccacaatgct tccatcattg
    14581 aatactagtg tcctaacatc agctggtaaa tctagttctt cagcatgtga gattgttaaa
    14641 tctgtgatga tcgttcctgg actccctttt gctagttgat aagctgtctt cctatcaaaa
    14701 acttcatgaa cattattacc gtaaaatgtc gtttctacaa tcgatcgaat actggataac
    14761 agtggatttc cacttgtgat ttcgtttaat tcgagactct cttttgtact catggctcct
    14821 cctcattccc taaacaaaaa aacgttttct ttattataca ttattcgagt ttttatttgt
    14881 actgctgcgc tgtgggaagc aaacgagtta tttttcacac aaaaaaaccg agaacttgaa
    14941 taaacaagtt tctcggtttc atcattttcc ttttatcggc ttagtaccag ccgtttgcta
    15001 accagaatgt tttagcagca tcccatgaac cataacgaga tgttacatat tgttctgcta
    15061 cacgttcttg attggcagct gaatagtcgc cgtttaagta tgaagcatct aattgataac
    15121 ggccgatgta acgtccgtta gtagctgtgt atgaaccgcc tgattctttt tgtgcgatcc
    15181 attcttttgc agagcttgaa gttggcgctg ttgcagcagc tggggcagca ggagctactt
    15241 caactgtttc aacaggtgca acgtatgtat gttcttgcgt tgctgtttca gttgtagatg
    15301 ctgtattttc tgttgttttg ttttctgttg ttgctgaaga atcatttgaa gttgggattg
    15361 ttaattgttc accaacataa atgcggttga tgtttgcgat cgaatttttt tcagcaattg
    15421 catcgatcat tgagttgtct cctgcaaatt tttgtgagat cttagataat gaatcacctg
    15481 attttactgt ataaacttca tcagcgtgag catttgtagt tcctacaaac attgcagtag
    15541 caccagcagc aaaagttgta cctaaaacaa ttttcttaaa tgaattcata ttaaactcct
    15601 ttttttctcc gttttttatt ctttcgacgc tattaacttt atcatttaga aatatttcat
    15661 aggtaaaaga aacatgacat ttaatgacaa aataacgaat tatttaatat ttgtaacatt
    15721 taaacaaata tttttttgat acaaaaccat aaaagccttg ttgtataagc aaagtaatcg
    15781 tttttctatg tttttttatt tttgaatgaa aaacgcagat tctgtaacat tcactgcaat
    15841 aatccataaa aaaaagcaaa aaaatttttg atttttatca aattctctta aaaaaatgta
    15901 aaaaatacaa aaaaaccagt aattgttaca acaattactg gacaaaaact ttgaataatg
    15961 ttaactattt ttttatttta actttctccg caataatttc tgggcatgtt ccaattcgtt
    16021 tataacgact tcttctgttt ctttagctaa tatttcatct aatacatcaa gccatttttc
    16081 cggttctttt gttcctagac gtccaatagc ccaagctgct gttcctctga tgacagggcg
    16141 gacatctgtt aaacattctg aaatctcagg caatgccgtt cgatcaccta gattagctaa
    16201 agcaatcaat gcgtttctct gcaaaggttt cttcccacgc catgaaccag ctaaatgccc
    16261 aaattgctgt ttgaagtctt tattagacat cgaaagcatt ggctttaatt ttggatatat
    16321 ttcgtctact tttggttcca tctcttcatg aaaatgaaaa tcttttcctt gattgtaagg
    16381 gcagactaac tggcagatat cacatccata aatgacattt cgcatttttt tacgatattc
    16441 ttccggcatc atcccttttg tctgagtttg ataagacaaa catcgctggg cattcattct
    16501 tccgtcacca agcaatgctt ttgtcggaca agcagtcacg cagcgcatac agtcaccgca
    16561 accaaattct cctggtttat caggctcaaa tacaatatta gtaacgatct caccaagata
    16621 gacaaatgaa ccgaactctt cagtgatcaa caatccgttt cgtccaataa atcctaaacc
    16681 agctcgctgt gctacagcga catcgatcag ttctccagta tctacttgcg gagctagacg
    16741 ccattcctct tctttttgcc atttttctgc tcttgtttgg atgaattcga tcaatcggtt
    16801 caaacggtct ctgagtatat catggtaatc gatcccccaa gaagcacgag caaactgccc
    16861 gcgtttttca tccctaggca cttcttcatg gatcctagta ggatacgcaa gtgcaatgga
    16921 aatgatcgat ttcggctgat caaatgtcat ttctggataa agacgctcat cgatatttgg
    16981 atgttcaaag ccagaagtat ggccagctgc tttttgttct tccaacgatt cttttaatga
    17041 atcaaaaggt tccgccgtgg tgaacccgat tttgtcaatt cctaatcgtt tgctttcttg
    17101 tatgatttct tcttttaatg tcatggtctt cctcgcttgc tgttaacggc tgtataaccc
    17161 ataatatagt ttttcttttt tcagctggac aaactggatc tgctgatcgg caatcttctt
    17221 ttcttccatc catttttttc ttgcagcaaa tacttcccaa taatttgttg aaattaattt
    17281 tcttttgtta tctaatgcat taaaaaaatt caatgaaact ggttttgtac ttaacagttc
    17341 atggagcctt acctcttccc atggcgaaaa ctcagacaga tcgcgggaat acaatacttc
    17401 ttcatagaga tttttttgct gtgtgtacgt gaatgtttgc gggattttcc caggcctagc
    17461 aatttctact agacgattat aaaacatatt cattaatttg ctgttggcaa taaattgact
    17521 tttataatag gccgactgga tctttttgat tactccttct gctttgatga ctgaaaataa
    17581 tgcaggccat gctttgacgg ttatttcgtc gttttgttta actaatacgc caggtgtgaa
    17641 gccgactgga gaaaggaaca caaaataacc gataccggta ttttcatcta aaggctgaaa
    17701 ttcatcaaac aaccatttgc ctatgtacag ctcttcatct ggttctgttg tctcattttg
    17761 atttgctatg aataatttag ctaattctgc ttcaagaaat tgaaggtctt gatccttgtc
    17821 ggtattttcc tgctggctga tcaatcgaaa gacttcttct ttaatcgtat atttatcttt
    17881 acgtgtaaaa ttcatagcca caccttcctt ccttactcta actataacgg aatttcttca
    17941 aaaaaggtat ctgtacgacg aaaaacagca taattttttt tcatatctct tataaaaaat
    18001 gcaaggtctg ctcctcaagc tagaccttgc acttttcagc tttcttttgt ttgtgacaaa
    18061 ccttcatgga tcgtttcaat attccatttc atcatctgat agtaagaatc tccttgttct
    18121 ccttcttttg ctatggaatc agtgaacagt ttatcataga ttttcaaacc cgtttctttt
    18181 gctacccgtt ccatgcttct tgagtctaca ctggtttcca cgaataaaac cggtacttct
    18241 tttgctctta tctgatcaat gatcgctttc atttgatcag gcgtaccttg actttctgta
    18301 ttgatctccc atatataagc tgctggcaaa tcatatgctc gtgaaaaata cttgaatgcc
    18361 ccttcacttg ttactaataa ttttttgttc tcttcgatag aagcaaaact ttccttcgct
    18421 tgtgtatcta attcttttag tttttccaca tatgcttctg catttttttt atagagtgtc
    18481 gcattttctg catctttttc agagaatata cgtgcgattt cctccacata ttttattccg
    18541 ttagataggt ctagccaagc atggggatct gcttttgtat gttcttcacc gctagttaaa
    18601 taaagaggtt ctacattttt gctaactgca aaataatctt ttccttcttc ttttttagcc
    18661 gtttccatca agttatcgaa ccagctgtta cctgtttcaa gattcaaacc gttgtataaa
    18721 ataacatctg catcgcttgc ctttttgatg tcttctggta gtacttcata ttcatgcgga
    18781 tctgttccga ctggtacgat actgtggata tctatttgat ctgttccgac ctcttttgcc
    18841 atgtccgcca agatcgaatt ggtagccacg actgacaact tttctttact tccttcatcc
    18901 gcttggtcag aagcctgctt tccgcaaccg gctaatacca agaaactaac tgccaagatt
    18961 aaaaagagtg atttttttgt tttcatctat ctgcttcctt tctgaaataa aattcctttt
    19021 gttggagaaa ataaaaaagc aaggataaaa aatgcggtac atgctaacac gatcgtagct
    19081 cctgatggcc aattgtaact ataactgaaa aacacaccga caactgcact tagcataccg
    19141 attcctgcag ctgtaatgat cattttcagt aaatggttcg ttagcaagta agccgtggcg
    19201 gctggtgtga tcaacatggc aatcaccaag attgttccta ctgtctgtaa actgactaca
    19261 gccactaatg tcaaaaagaa catcaaaaga taatggatca gcgatgtgtt cagcccataa
    19321 gcttttgcca ttgtcggatc gaatgatgtg atcttcaatt ctttataaaa gaaaaagaca
    19381 aatatcaaga cgattccact aacgagtgct gtcagcaaca aatcgctttc cctaacagct
    19441 aagacattcc caaataaaat atgatacaaa tctgtcgcac tctgggcata tgaaatcaag
    19501 ataatcccta atgcaaaaaa ggagctaaag acaattccaa tcgctgtatc atttttcaat
    19561 cggctccgct gagtcacgaa accaatcact gctgctgtta gcatcccaaa agcagtggct
    19621 ccaaagatgt aactaaaacc aaacatataa gaaatggcta cacctggaag aactgcgtga
    19681 gagatagcgt ctcccatcaa agacatccct cgtaaaataa taaaagaacc gatgactcca
    19741 gaagcaagtc ctacaagcat ggaagtgacc aaagcgtatt gcaggaattg ataattcatt
    19801 aatccatcaa taaaggattg gatcattttt tctctcctcc ttttactaaa tgtctgatga
    19861 tctcaccata ggccaattga aggttcttcg atgtaaagac ttcttgtact ggaccggaag
    19921 cgatcagctg tttatttaaa agaataacct catcgaaata ttcttctact ttatgaagat
    19981 catgatggac gatcaaaatc gtttttcctg aattcttcaa ttcctgcaag atgtcaaaga
    20041 tctttcgttc acttaacgca tcaatcccta cgaatggttc atctaaaaag atccattctg
    20101 ctccttgggc tagagctctt gcaataaaaa ctctctgaag ctgtccaccc gatagttcgc
    20161 tgatctgtct tttggcatat tcttccatcc ctactttttt caaagcttgt cttgcacgtt
    20221 ctttttcttt tttcccaggt ctttgtccaa ttcgtaaaga tggatatgtt cctaaaagta
    20281 caacgcctaa tacatcaatt ggaaaggaaa gatccaattc acttcgctgt tccacgtagg
    20341 ctattttttt acggactgag tttaccggct gtccagaaaa ggtgacttta ccagtagtag
    20401 ctggtatcaa ctctagcaaa gatttcataa atgttgattt tcctgcaccg tttggtccga
    20461 taataccggt aattttttgt ggatttatcg tcaagttgat agttttcaaa acagttttcc
    20521 cttgataagc ggctgaaagg tcttgaatta caatggccgt acgttccaaa tcttacactc
    20581 ctttttaggt ttacctaact ttttatttaa atatagttta actaaaatgg aaagcatttt
    20641 caagtagaat gatgaaaaag tttttttcga attttcctcg cattttatga aaattttctg
    20701 tagaatcaca tcaagagttc aataaggcta acctaataat gaaaaacatg tgttgaaacg
    20761 tttgattatt ttttcttgtt gactcaacaa cgaaaggagt ttttgtatgg aagaagaaaa
    20821 gttaactcga agtcttagtg cccgtcatat ccaaatgatc gcactaggtg gaacgatcgg
    20881 tgtcggtctg tttatgggtg cctcctcaac catacgttgg acagggccct cagtgatggg
    20941 agcctatgcg attgcaggat tctttttgta tttgattatg cgtgctttag gcgaaatgct
    21001 ttacgttgat ccttcgactg gttcttttgc aaattacgcg agcgagtaca tccatcctgt
    21061 ggctggctac ttgaccgctt ggagtaatat ttttcaatat attgtcgtag gaataagtga
    21121 agtgattgct gtaggatctt atatgaatta ttggtggcct gatttgccgg caattattcc
    21181 cggaatcatt gtcgtcttgt ttcttatgct ggcaaatctt atttctgtca aggcgtttgg
    21241 cgaattagaa ttttggtttt cgatcatcaa agtcataacg atcattttga tgatcattgc
    21301 aggtcttggg gtaatcttat ttggatttgg taatcatggt caggcagtag ggatttcaaa
    21361 tctttggaaa aatggtggat tcttcactgg aggcgtcaaa ggatttttct ttgcattatc
    21421 gatcgttgtt gcttcctatc aaggaatcga actgatcgga atgactgctg gtgaagcaga
    21481 gaatcctaaa aagacgatca ttgaagctgt ccagtccact atcggtcgaa tcttgatttt
    21541 ttatattggt gctattttta tcattgtgag tatctatccg tggaatcaat tgagtgagat
    21601 tggttcacct tttgtacaaa ctttttctaa agtcggcatt acatttgctg caggactgat
    21661 taattttgtc gtgattacag cggcattatc aggttgcaat tcagggattt tcagcgcaag
    21721 tcgtatggtc tatacacttg ccataaatgg aaagatgtct aaaaaatttc tgaaactatc
    21781 tcgtcacggt gcgccttttt atcctgtcgt agcaatctca ggtggaatct taattggtct
    21841 gatcttaaat tatcttctac caatgttcat caaccagtca gaaaatattt tcgttttcgt
    21901 atacagctca agtattttgc ctggtatggt tccttgggta gtgattttga tcagcgagat
    21961 tcgcttcaaa aagaagcacg cagataaaat gcatgatcat ccttttaaaa tgccactatc
    22021 tccttatacg aattatgtga cacttgcttt tttagctatc gtattgattt tcatgtttat
    22081 caatcctgaa acgagaatct cattgattat tggactcgtt ttcttgcttt acatgacgat
    22141 ccattacttt gtacgcgaaa aaagaaccgg aaaataaaaa aagaacgaag cttcacctta
    22201 gtggaagctt cgttcttttt tattgctttt atcaattcaa ttttagcgtc tcaccgggag
    22261 tcaggaaata attctccatg ctcatcccat tcagcgacaa gatcgtttct actggtacgc
    22321 cagttcgttc ggctatttga tttggacctt ctccttccaa aactacggtt gtcgcagcct
    22381 gtgaagtcgc gctgttggta ttagtttcag aggaatctgt tgtgtcttgt gttgttcgag
    22441 cttgcatttc agaagcttct accgactctg ctgtctcaga atcagaagat gttgtgtctt
    22501 gtgtgcttgt tgactctgct tgaagagagg cgctagtctg caaataaata tcggaaatct
    22561 tagcgacatg attcgtataa ttagccgata attgattgat caaagtatct aagtctacac
    22621 cgtctttatt ttctgctgcc tgatacgctg cacgtaattt tctgatccct tcgtcggaga
    22681 ttgcttgaag ctcacgtgct ttttggttag caactgctga taaaccagct attccctctt
    22741 gatttccctg aatttctgtc tggtattctt caatcaatgc aggcgtcttt tcttcgatct
    22801 ttttcgaata atcatttacg attgcttgaa gttgctctgg caaaccattt gctgtttttt
    22861 cttcagaagt ttgtgtctct tttgttttag ttgatgactc acttgtttgt ttcgtctctg
    22921 ttttctttgt cgttgtttcc gttatggtcg tacttttcgc ttctgacgaa ttttttccat
    22981 gaaaatatag acccgcaact cctcctgcta tgatcaaaaa cacggcaact gctgccccaa
    23041 tcatgatcat ccgcttacga gctgcctgtt tttgctgtct tcttcgggtc atccgaacat
    23101 tttctcccca ctcattttca tcatacattt ttttcaccac caatttttat gtataatagc
    23161 gttatcaaac gcttcatatt cttattgtag caaataattg tcgctgtgca catttttatc
    23221 tttgttattt ataagaaaat ttcaccagtt gagatgattc caagattgtc cgtcgatttc
    23281 tccttgcagc tgcattgttc gttcgtctat ctcagccatc agtttcttta tttcatataa
    23341 gagcgcacgg tcttgatatt ctgatttttc gcttaattca tctaattgtt ggatcagcca
    23401 atcatttgtc ataatgaagt tcctttcatt tcttgcagtg taaaggtcaa gtcttcgatc
    23461 tgctcttttg cctcagtcag tgcttctacg atatatgctt gtttttcaga ttcttcttcc
    23521 tgcattttct gattttcctg tactctttga ataaaccagt caatccgctg agtcagcgat
    23581 ggtttgcctt ccaatgagag atactgctga taagcaatta gcttctccca acgtttttcc
    23641 tgggataaat acataaattg tgctaaaacg aatggcggaa gataggtcat atggaattta
    23701 tttgcgattc gctgatacgg cctcaaaaat tcggaaatcg tatattcttc ttttcctcca
    23761 gcttggtatt ctttttccgc cacacctgtt gtcaatacga ttcctagttc tttccctttt
    23821 aatacatgtg ctgctcgttc caagacatta tcttgccaaa tttttaaatg agcaggagaa
    23881 ctataccagt aaaacggaaa ctggaaaatg atccgatcat gctgggccaa gagctgtagt
    23941 tcagtctcta tatctatttt tccgtctgga taacttgatt ccaaatgatg ccaagtagct
    24001 ggtaaaccag cagaagcttc tttgaaaaat tgctgattta aagattcttc gatcgttgga
    24061 tggctaatga tgataagtgt acgcatccct tttcctcctt tttagtcttc gctttacagt
    24121 gtagcataac cgccacaagt ttcttttgcc gatgcttgtc attttaacca gactaatcag
    24181 gttgtccaag aaactgcggt ttgtttttcc aactgttttt tcaaggtatc caaagcaata
    24241 ttcttaaaat tttcgttttt catacgttta cgatagttcg tagccgtttc tccggtactc
    24301 tttttgaaca ttcggctaaa tacgactaga ctattgaacc ctacttgata ggcaatactt
    24361 tccacggatt catccgtcag agaaagaaga aacgcccctc gattgatccg taccccgatc
    24421 aaatattcct tgatactggt gcccatatga tttttgaata ctcgcgacaa atggctccga
    24481 ttgaccgcta aatgctgggc gatttcctca acagacaatt tttcagcata atgtgcctcg
    24541 atgtatttga ctgtttcaac agctaaagag gaatgaacct tttgtacacc tccgctgacc
    24601 tttccgccat cttttaaaag gactgataaa aaacggtagg ttaatgctgt taactgtaat
    24661 tcattttcca aggtttcttc cgtatctttc atgcattcta aaataatgtc gatatatttt
    24721 tgtatgtcag aagagaccat cgtgtattgg tcttctctga aaagcgactg ccgaatgatt
    24781 tcttctgcag cactcccacc aaatgaaagc cagcaataca tccacggatc ttctccatct
    24841 gataagtaaa acgtcgagtc tcctggacga atcaagaaca gatcaccttt tttcaattgg
    24901 aattgttgat ttttcacatg ataagatcct ttgccttcca tcacgacatg caggatgtaa
    24961 tcattgcgaa tagcaggacc aaaagaatga aacggcatcg ttttagagaa accacaaaaa
    25021 tcaaaataca attctttttc tacttgttga tgttcctttt ttaaaaaaat cgattgctcc
    25081 acgtctatcc ctccttactc ctttagtata atgcaacaaa aagttaaata aaagtcacag
    25141 aaatatatta aaaaggaagc gatttcttgt taagtttaag tcattaaaag atctttgtta
    25201 ttcgttgtaa ccggtttact tagtattata tattgaacag gagaggaata cagattatga
    25261 aaagcaaaat gatccaacgt gtgtcctacg ctttaggcgc ttttggacac gacacattct
    25321 atgttacatt aagcacttat tttatggtat tcgtgacaag ccagatgttc gcaggaacag
    25381 ataaggcaac gaatgcaaaa atgattgcta cagttacaag cttagttgta ggtatccgct
    25441 tagttgaaat tatttttgat cctttgattg gtggcatcat cgataatact cgtacaaaat
    25501 acggtaaatt caaaccatgg ttagttatcg gcggcgttgt tagttccgta ctcttggtag
    25561 caatctttac caatttcttt ggcttagcta ctcataatac gactttattt atcatattat
    25621 ttgcaatcgt tttcatctta ttggatagtt tttattcatt taaggatatc gctttttggt
    25681 caatgattcc agctttgagt acggaaacaa aggaacgtga aaaatttgct acgtttgctc
    25741 gtttcggctc ttctctaggt gcaaatggta ctactctagt cgttgtccct atcgttactt
    25801 tctttaccta tcttgttact cataaacata ttgaaggcgc ttcaggctgg ttctggttcg
    25861 gtgtgattgc tgctgttatt tcaggcggta ctgcgattat cactgccttg ggcacaaaag
    25921 aagtcgatac tgcgatccgt cagcaaaatg aaaaagctgg gatcaaagat gtgttcaaag
    25981 cagttgctca aaatgatcag ttgatgtggc ttgcccttgc ttatatcgct tatgcgattg
    26041 ccaatgtagc aacaactgcc gtattattct acttcttcaa atatgtactt ggccaaccaa
    26101 ctgaattttg gatcgttggt ctagttgctg ccttacttgg tttagtagct gttccactat
    26161 tcccaatgct tacacgcgtc atctctcgtc gctacgttta ccttggaggg atcgcaatga
    26221 tgatttcttc ttactttttc tttacgatcg caggttcaag tttgccaatc gttatcatcg
    26281 ggctagtatt gttctacttc cctcaacaat tgattttctt atccgctttg atgacgatca
    26341 cagactctgt tgaatatggt caatggaaaa atggtatccg taacgaagct gttactctat
    26401 caattcgccc tttactagat aaaatcgctg gtgctttgtc taacgggatc gtagggtttg
    26461 tcgctatcgc ttctgggatg actggtaatg ctactgctgc tgatatcact gctcatggcg
    26521 taacgacttt caaagcttat gcattctatg ctcctgctgc tttgatgatt ttctccgcta
    26581 ttatttttgc ttggaagatt acattgacag agaaaaaaca tgcacaaatc gttgagaaat
    26641 tggaaaacaa attggctcca gctgatacaa tggaagatga acttcacgat gcagtagttc
    26701 cttcccatta agaaaggatc ttgaaaatga agattacaga aaaagatttt ggccaaggtt
    26761 atcacttgat aaccttggag aacaaaaaca agctagcact ttctatctct gatttaggtg
    26821 ctagaatcgt cagtttaaaa tcaaatgatc gagaattagt tttgggattt gacaccgctg
    26881 aagaatatat cgaaaaagat ccgtatatcg gtgcttctat cggtcgcacc gcagggagaa
    26941 tcgaaaatgg ccgcttttct ctcaatggaa aaacttacca attagccaca gatccaaaaa
    27001 ctggacataa tctgcatggt ggtgctcctg gatttgaatt aaaaaaatgg tcttatgtca
    27061 ttttgaatgg cgaaaatgaa gcttctgtca tttttactac tacaagtccc gacggagaac
    27121 atggttttcc aggtacaatg gatgtggaaa tccgctatac tttgacaaaa gataatatct
    27181 ggcgagtgac ctctcgcggc accagtgacc aagatacttt attcaatccg acaaatcatg
    27241 tttacttcaa cttgacggga gatgcttctc agtcaatcga tcaacatgag ctttggctca
    27301 atagcgaagc ttatgctcct ttgcgaacag atagtattcc tataggggta aaagaaaatg
    27361 ctgcaggaag tgctttcgat tttcaaatac ctaaaaaatt agcgtccgtt tttgctagtg
    27421 atttggatca aaaaaatctt gttgacggaa tcgatcatcc atttttctta aaagaaaccg
    27481 gcttgggtaa agaagccgct cgcttgacta gccccgataa aagaatccaa gtagatattg
    27541 ctacagatgc ttctagtgtg gttattttca ctgcaaattt cggcacagaa acaccagaaa
    27601 tgcgcaaccg aaaactggca catcacggtg gcatcacatt tgagactcaa acagctccag
    27661 gtgcagagcg cttctcagca tttggttcga ttcatttaaa agccggatca gtatttgaaa
    27721 cagtgacaga attcaaaatc aaaacaagaa aggaataact catcatggaa atcaaaagaa
    27781 gcttaaacac aaaatttgcc gaaacattcg gacctaaaaa aactacacaa tacttctcac
    27841 caggacgcat caatttgatt ggtgaacaca cagattacaa tggaggccat gtcttccccg
    27901 cttccatcac ttatggaacg caaggtgtag cagcacctcg agacgataat aaaatattag
    27961 tttattccac aaactttgac gatatcggtg tcattagttt tactttagat gagctagaat
    28021 atgataaaaa agcaaactgg gcaaattacg taaaaggaat ggtccttaaa ttaaaagaag
    28081 ctggacactc aatcgatcat gggtttgagt tattagtgga aggaacaatc cctaatggtg
    28141 caggactttc cagtagcgct tcattagaat tattagtagg tgttgtatta gaagatctgt
    28201 ttgatttaaa aacagatcgc ttggaactag tccaaacagg taaaaaagta gaaaatgaat
    28261 tcatcggtgt caattcaggt atcatggatc agtttgccat cggcttcggt gagctagatc
    28321 atgcaatctt attagataca aatacgctga aatacgaaat ggttccagtg aaactagatg
    28381 gttatgcaat cgtcatcatg aatacgaata agcggcggga attggcagat tcaaaataca
    28441 atgaacgccg tagcgaatgc gaagaagcct tggcacgttt gcaaaaaact ttgcctatct
    28501 cttctttagg agacttagat gaagaagaat ttgaatcaac tattgaccaa atcggcgatg
    28561 atacattgat acgcagagct cgccatgccg tttctgaaaa tcagcggact ttgaaggcaa
    28621 aagctgaatt agaagctggg aacttagaag catttggtca actgttaaat gattctcacc
    28681 actctcttcg ctatgattat gaagtaactg ggatcgaatt agatacacta gtagatgctg
    28741 ctcaaaaaca agaaggtgtc ttaggtgcac gaatgacagg agctggattt ggtggctgtg
    28801 cgattgctct tgtcaaagaa gaaaatatac ctgccttcaa aaacaatgtc tatgatgaat
    28861 acatgaaagt tgtcggctat gctccggaat tttatgttgc acacattgga aatgggacaa
    28921 caaagatcga tccagaagct gttcatgttt gataaaaaaa tcaacgatct cagctaaaaa
    28981 caaatagata aaaataacct ctcagcaatt tccaaatgtg agccttttgc tcgtatttga
    29041 aaattgctgt tttttattga cacggaaaga gaacgtgtgt tctaattgtg ttgaagaggt
    29101 gattgttatg tgggcgaatc aaaatccggt atttgtttat gaaaaagaac cttcaagaga
    29161 tattttatgt attgactgca aatcttttta cggtgcgaaa cgttcctaac tgaaaaggtt
    29221 aggcacacaa gattttcttg tgtagaacta atgatttgag gagtgaaatg acggtgtaac
    29281 gacctgaagc gctccctctg atactccgat tggcaatacc ttaagttaga ataggaattg
    29341 tcaaaagctc ggtaaagtcg gctgagagtt accgcagttg cattatgcaa tcgaaagctg
    29401 ttcagcggtg aatggtgtaa cctataggcc gggagtccag aaattactca tggtgagaat
    29461 gtgcagctgc actgacgaac tcgcgaatgt acgaatctat aagcttgggg tacagaaatg
    29521 tatcttaccc gcacaaatat tactgtgaca aagggtgtag cttgcggtta agtaagaatc
    29581 gtagatatga acaaccgtgc aatgtagtag gcattggaaa gttacaggtt cagagtgagc
    29641 gcctaaaagt aaatgcaggg ataaaatcat tggaacgttg aaagctagtg tcgtggagga
    29701 ctaacttcct ttgaaacggt tgagacgaag aataataagc tcatttacac tagtgagagt
    29761 agtggcacag tacctatgaa gcagtgataa taagctgtgg agggatagcc acaagtcaat
    29821 ttgctttact aacagttatg atacaatcca tagaatcgag tatgacaaaa taaacgccga
    29881 cgtccgaaag gaggcggtgc tatggaaaac gtatcagcat cacattcaat tcgccatatg
    29941 gaatattatg gtcttattga tagctttgat tctctttaca aagagagtag cgagaacaaa
    30001 attttcagta accttatgga aattgtcact tctcctaata acattctatt ggcatttcgg
    30061 aatgttaaag gaaattctgg tagtacttcc ccaggcgtcg acaaaaagaa tattgatgat
    30121 ttaaaagcaa taccgaatat cgagtttata aaaactgttc aaacaaaatt ttcagaatat
    30181 aaaccgcaac cagttaaacg agtagatatt cctaagccaa atgggaaaac tagaccacta
    30241 ggtataccaa caatatggga tagaatcata caacagtgtc tactacaagt tttagaaccg
    30301 atcatggaag caaagtttca tgataagaac tatggtttcc gacctaaccg gtcggcacat
    30361 catgcattcg ctcaagcagt acgaatggcg caactttcaa aactaacttt tgtagtagac
    30421 atagacatag aaggattctt tgataacgta aaccattcaa aacttattaa acagttttgg
    30481 acattagggg tacgtgataa atggcttcta ggtgttatcc gagcaatgct taaagcacct
    30541 attattcata gggatggacg cattgaacat ccaaagaaag gaaccccgca aggcggtgtt
    30601 ctctctccac ttcttgccaa tgttgttttg aatgaactgg actggtggat atcttctcaa
    30661 tgggaaacac acccgactag gaaaaactac gattgttatc atcaaacaag aaagcaatgg
    30721 attaagagca acaaatatcg agcactgcgt gcttcgaaac taaaagagat ttatattgtt
    30781 agatacgcag atgacttcaa aatcttctgt agaaaacgaa gtgacgcaga caaaatcttt
    30841 ctagccacta aattatggtt aaaagaccga ctgaaacttg atataagtgc tgaaaaatca
    30901 aaggtagtga atctaaagaa acagaaatct gattttctcg gattcacaat gaaactggta
    30961 cgcaaaagaa aaagctttgt gattgaaacg cacatgtgcg ccaaagccat gagtgcagca
    31021 tctaataagt tagcaaaaca aattaaggtg attcaacatt cacctaccca agttgaactt
    31081 caaaaacagt taagtattta taactcaatg gtagtaggta tccatcaata ttataaaaag
    31141 gcaacccaca tcagtgtgga ctgcccaaga ataaatcaac gcctaatgat tatcatcaaa
    31201 aatcgactag atattaagag aaagggtcct ccaccgagtg attttattaa aaaaaattac
    31261 gctcggtcaa agcagttgag gtggcttaat ggtgttccta tgcttccttt tggattcata
    31321 aaacatagcc acacctccct gcccaagata ggaacctgta aatacacgcc cgaaggtcgt
    31381 gtgctgatac acaggtatct tactttccca atcgatatta tcaaccaact catgaacatg
    31441 tctaatccta atgaaagcat tgagtttaga gacaatcgtg tatccaagta ctcttcccaa
    31501 gtcggccgtt gccgagtaac gaaagaagtc ttagagctag cagacatata ctgccaccgt
    31561 attgtcccta aaaatctagg tgggaaagac aattatcaaa atctggtcat tgtccaccgg
    31621 aacattcact cacttattca tgaagaggat aaaacaagag catcacttct catacaaaac
    31681 atgagagtta caagtgctca acttgagata atcaaccagt ttcgcaaaaa agctaagaat
    31741 cgtgaattgt cgctgaatct ataactgtat gatactgtta gtaaactttg atggaacgcc
    31801 gtatgcggtg aaagtcgcac gtacggtgtg gagcggggga aaatctggag attgcatcaa
    31861 aggattacct atcgctatcc tccgtagagt gcgttgaaaa ggatcttgat ccccttacaa
    31921 ctaaattagt agtcatgtcc tatccgtccg attcgacaga aacacggggt agcggattaa
    31981 tcttggcttc cagtcctgca gcaaaaaaag cttacggcat caccaatatc agccgtgcca
    32041 gagatcttcc cttcccctat ccaagtgatt tatatattgt tgccccaagg atggcctatt
    32101 atatggaaaa gaacagagaa atcaattcta tctataaaaa atatgcagac gaagcgaacc
    32161 atcatgtcta ttccgtcgat gaaagcttcc ttgatgtaac aaatagcatg gcactctttg
    32221 gtaaaacaaa tgcgtacgac atggctaaac tgatccataa agatgtgttt gagcagaccg
    32281 gaatctacac gactatagga attggcgata acccgctact tgctaaacta gccttagaca
    32341 atggctcaaa agacggaaag caaatgtttg ctgaatggcg ttatgaagat gtaccagaaa
    32401 agttatggaa aatctcagat atcactgact tttggggaat cggcaaacga acagcaaaac
    32461 gtctgaaaca aatgggtata cattctgttt atgatttagc tcacgcggat tattatcact
    32521 tgaaaagcaa gctaggggtc atcggtactc agctttatgc ccatgcttgg ggcatcgatc
    32581 gaagtttttt gggacaagaa tatacaccaa aatcaaaaag tatcggaaat agtcaaatcc
    32641 tagatagaga ttatacaaga cgagaagaaa tcgagatcgt tatcaaagaa atggccgacc
    32701 aagtcggcac tcgcttgaga cgatctgcag caaaaacaca agtagtcagc ttatgggtag
    32761 gatactcttt aggctatctt gatccaaatg gcaagaccgg gtttcaccag caaatgaaaa
    32821 tcgaagcgac aaatgctagc agagatctag cagatgcttt attgaccatt ttccaccagc
    32881 attaccacta ccaggatatt cggcgtgtag gcgtgaactg ttccgacctc gtatacgcga
    32941 caggtttaca gcttaacctt tttgaagatc ctgaaaagca gataaatgaa gcgaaagttg
    33001 attttgttgt cgatacgatt agaaaaaaat acggctttca atcaatcatc catgcgcata
    33061 gtctcttgca aggaggacgc gccatcgcac gtagttctct cgttggcggg catgcaggtg
    33121 gtatgagtgg attagaaggt ggaaaagatg agacggacag caaaaaaatt cattgactat
    33181 aacgagtatc gagatcggcc atttggatta aaatggggga ctgcttttgc gatggaagaa
    33241 ctagtcaacg gtatcaaaaa aaatgaagaa gaagcacgca aacagcctct tcctaaacaa
    33301 caaatgacat tgcaagaaat cgatagtata ctgacagaag cctttttatc tcgtaaagca
    33361 gtaatcattc agctcaattt aaaagatgaa tttggaagac tcttggatga ggtcgaaggg
    33421 cacttcattg gtgaagccta tgaagattac tttatgattg accatcagcc gatattgtgg
    33481 gaagacgtac ggtatattga gttaaaggaa acaaaaaaat ggtttgtgac atgagaggtt
    33541 gtgacagaag tgatcaactt caaaaaataa gaaaggattt ttcgaaaatt gcttcttaaa
    33601 tttttgtgga attttggctt atttttcgaa gaagcaactt ctggaacaac gtatattaga
    33661 tatcagaggt tgtgaaaaag tcgcttagtt ttgagtaaca agccaaggcg ctgatttcac
    33721 aacctctgtc tatctatcca tctattcggt aagattcatg catgacgtct tctttattta
    33781 gtatgtagag ccctttttct tctgaccatt caacataaat cagctgtttt acatctgcat
    33841 agccttcttt cttcaagttc tccataagcc atgcttcagt caattgattg gactgaagca
    33901 ctttatgctg aatttgccct tcatctacaa gcaggatcga taaagctttg tcttcatctg
    33961 attttttcaa tacactcatt tgaccggcat tctctaggat aacatgtttg gcattttcaa
    34021 gaaaaaagca ttcttgctgt cttaagattg ctcgcaactg ctccatctcg atatggttat
    34081 ttgtcaactc tgtcatgttg atgacgccgt ctttgattag aacagatggc tcgcctttga
    34141 cccaacgatt gaccttttca tttttttgga cgatacgttc gataccataa atcattactg
    34201 cccaaagcgc aatggcaaat aggacatgcc ctacatggac attgtcatca taggttgatt
    34261 cttctaagat cccacctaaa acaagcgtat agactaaatc aaacggtgtg atttctgaca
    34321 tggatttctt cccaagcaaa cgtaccacta ataataaacc gatcaacccg aacaccagtt
    34381 tcattgtaat tgaaaaaatc attttttctc cccctaacta tctgttaatt aaattatata
    34441 tgctttctca cgaacacgca tggaatatac aaggaagaaa tgtatccttt tactaaaaaa
    34501 aggactgtga cactgtagtg acccccaaaa gttagacttt tttggagtgg agaaatccac
    34561 tccttttttt tatgctctaa aatgatattt ttacacaacc gttacggttg ttttacgaaa
    34621 ttggacg
//
LOCUS       DAZABD010000027        34590 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000027 DAZABD010000000
VERSION     DAZABD010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34590
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.027"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            212..604
                     /locus_tag="ACHALG_001344"
     CDS             212..604
                     /locus_tag="ACHALG_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFN2586764.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="ACHALG_001345"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="ACHALG_001345"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFN2586765.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            2698..2943
                     /locus_tag="ACHALG_001346"
     CDS             2698..2943
                     /locus_tag="ACHALG_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2586766.1"
                     /translation="MKEKDCFYDKLYKLLLQVGKSSSLLGGILSIIMFVLFFYLLFHL
                     KFFSNDFLICLGLTLAMFISSLLCGLNFFCTHKGLKK"
     gene            3164..4774
                     /locus_tag="ACHALG_001347"
     CDS             3164..4774
                     /locus_tag="ACHALG_001347"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFN2586767.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(4751..4963)
                     /locus_tag="ACHALG_001348"
     CDS             complement(4751..4963)
                     /locus_tag="ACHALG_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2586768.1"
                     /translation="MKMKIFKKFFAKNKISNSALIEKSLLLSIFFPTNNNKSEKEKSD
                     KKIAYPLLLLLFSFYQVQKLFCFVIC"
     gene            5066..5935
                     /locus_tag="ACHALG_001349"
     CDS             5066..5935
                     /locus_tag="ACHALG_001349"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFN2586769.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6100..7371
                     /locus_tag="ACHALG_001350"
     CDS             6100..7371
                     /locus_tag="ACHALG_001350"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFN2586770.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7368..8663
                     /gene="rho"
                     /locus_tag="ACHALG_001351"
     CDS             7368..8663
                     /gene="rho"
                     /locus_tag="ACHALG_001351"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFN2586771.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8762..9025
                     /locus_tag="ACHALG_001352"
     CDS             8762..9025
                     /locus_tag="ACHALG_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFN2586772.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9470..9919
                     /locus_tag="ACHALG_001353"
     CDS             9470..9919
                     /locus_tag="ACHALG_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFN2586773.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9976..11394
                     /locus_tag="ACHALG_001354"
     CDS             9976..11394
                     /locus_tag="ACHALG_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFN2586774.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11394..12254
                     /locus_tag="ACHALG_001355"
     CDS             11394..12254
                     /locus_tag="ACHALG_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFN2586775.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12428..13201
                     /locus_tag="ACHALG_001356"
     CDS             12428..13201
                     /locus_tag="ACHALG_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFN2586776.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13348..13713
                     /locus_tag="ACHALG_001357"
     CDS             13348..13713
                     /locus_tag="ACHALG_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HFN2586777.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13861..15069)
                     /locus_tag="ACHALG_001358"
     CDS             complement(13861..15069)
                     /locus_tag="ACHALG_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFN2586778.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15088..15366)
                     /locus_tag="ACHALG_001359"
     CDS             complement(15088..15366)
                     /locus_tag="ACHALG_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2586779.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15559..16215
                     /locus_tag="ACHALG_001360"
     CDS             15559..16215
                     /locus_tag="ACHALG_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFN2586780.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(16657..17454)
                     /locus_tag="ACHALG_001361"
     CDS             complement(16657..17454)
                     /locus_tag="ACHALG_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFN2586781.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17451..18635)
                     /locus_tag="ACHALG_001362"
     CDS             complement(17451..18635)
                     /locus_tag="ACHALG_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HFN2586782.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18660..19907)
                     /locus_tag="ACHALG_001363"
     CDS             complement(18660..19907)
                     /locus_tag="ACHALG_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFN2586783.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20122..21867
                     /gene="dnaX"
                     /locus_tag="ACHALG_001364"
     CDS             20122..21867
                     /gene="dnaX"
                     /locus_tag="ACHALG_001364"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFN2586784.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21887..22201
                     /locus_tag="ACHALG_001365"
     CDS             21887..22201
                     /locus_tag="ACHALG_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFN2586785.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22226..22822
                     /gene="recR"
                     /locus_tag="ACHALG_001366"
     CDS             22226..22822
                     /gene="recR"
                     /locus_tag="ACHALG_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFN2586786.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22886..23530
                     /gene="tmk"
                     /locus_tag="ACHALG_001367"
     CDS             22886..23530
                     /gene="tmk"
                     /locus_tag="ACHALG_001367"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFN2586787.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23545..23874
                     /locus_tag="ACHALG_001368"
     CDS             23545..23874
                     /locus_tag="ACHALG_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HFN2586788.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23888..24826
                     /gene="holB"
                     /locus_tag="ACHALG_001369"
     CDS             23888..24826
                     /gene="holB"
                     /locus_tag="ACHALG_001369"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFN2586789.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24862..25686
                     /locus_tag="ACHALG_001370"
     CDS             24862..25686
                     /locus_tag="ACHALG_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HFN2586790.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25679..26026
                     /locus_tag="ACHALG_001371"
     CDS             25679..26026
                     /locus_tag="ACHALG_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HFN2586791.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26095..26967
                     /gene="rsmI"
                     /locus_tag="ACHALG_001372"
     CDS             26095..26967
                     /gene="rsmI"
                     /locus_tag="ACHALG_001372"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFN2586792.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27076..28197
                     /gene="dinB"
                     /locus_tag="ACHALG_001373"
     CDS             27076..28197
                     /gene="dinB"
                     /locus_tag="ACHALG_001373"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFN2586793.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28251..28853)
                     /gene="nrdG"
                     /locus_tag="ACHALG_001374"
     CDS             complement(28251..28853)
                     /gene="nrdG"
                     /locus_tag="ACHALG_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HFN2586794.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29168..31357)
                     /gene="nrdD"
                     /locus_tag="ACHALG_001375"
     CDS             complement(29168..31357)
                     /gene="nrdD"
                     /locus_tag="ACHALG_001375"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFN2586795.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31683..32450
                     /locus_tag="ACHALG_001376"
     CDS             31683..32450
                     /locus_tag="ACHALG_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN2586796.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32440..34482
                     /locus_tag="ACHALG_001377"
     CDS             32440..34482
                     /locus_tag="ACHALG_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN2586797.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
BASE COUNT        11118 a         6130 c         7259 g        10083 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga atttcggctc cctattctta ttgaagaata gggagctttg tttattgtct
     3121 gagacttttc ctgcacataa attaaaaagg agtttttgaa cttatgacaa aatatatttt
     3181 tgtgaccggc ggtgtcgttt catcaatcgg aaaagggatt gttgcagctt ctttaggacg
     3241 cttattgaaa aaccgcggct taaaagtaac tatccaaaaa tttgatccat acatcaacgt
     3301 ggatccgggg acgatgagcc catatcagca cggagaagta ttcgtaacag atgatggtgc
     3361 tgaaacagat ttggatctgg gccactacga acgttttatt gacatcaatt tgaacaaata
     3421 ctccaacgtg acgacaggaa aaatctattc tgaagttttg cgtaaagaac gtaagggcga
     3481 atacttggga gcaactgttc aagttattcc tcatattaca aatgagatca aagaaaaaat
     3541 catgcgagca gctaaaatga ctgattcaga tgtgatcatt acagaagtag ggggaacagt
     3601 aggggatata gaatctttgc cattccttga agcacttcgt caaatgaaag cagatgtcgg
     3661 tacagataat gttatgtata tccatacaac attgattcct tacttgaaag ctgctggaga
     3721 aatgaaaact aaaccaacgc agcacagtgt aaaagagcta cgtggtttag gtatccaacc
     3781 gaatattttg gtggtacgta cagagcaacc tgtttctcaa agcgtgaaaa acaaattggc
     3841 acaattttgt gatgtcgaac cagaagcagt tatcgagtcg ccagatgtag atacacttta
     3901 ctccatccca ttagttttac aagcacaagg aatggatcaa attgtttgcg accatttaaa
     3961 actagataca ccagaagcag acatgacaga atggaaagaa ttagaagaac gtgtcttgaa
     4021 cttgaagaaa aaagtacgga ttgcgcttgt cggtaaatat gtcgagttac cagatgcata
     4081 tatctcagta gttgaagcat tgaaacattc tggtttcgcc tataattcag acatcgagat
     4141 cgattggatc aaagcacaag aattaacgag agaaaatgta gaagaacgtt tgaaagacgc
     4201 agatggaatc ttggttcctg gtggatttgg tgatcgcgga gtagaaggga aaatcgaagc
     4261 gattcgttat gctcgcgaaa atgacgttcc gtttttaggt atttgtcttg gtatgcagat
     4321 ggcttgtgtc gaatttgcac gtaacgtcgt tggattagaa gatgcgtcat cagctgaaac
     4381 gaatccagat acagcaaaca atattattga tttaatggct gatcaggaaa acatcgagaa
     4441 tttgggtgga actttacgcc ttggattata cccatgtaaa atcaaaaaag gaacgaagac
     4501 ggctgaagca tatgatggag ccgatgttgt gcaagaacgt caccgtcacc gctatgagtt
     4561 caataacaaa tatcgtcaat tgtttgaaga acaagggcta gtcttttcag gtgtctcacc
     4621 agataaccgt ttagtagaaa tcgtagagct gccagagaaa aaattctttg tgggatgcca
     4681 attccaccca gaattgattt ctcgtccaaa tcgcccgcaa aaattaatca aagcatttgt
     4741 cggagcatca ttagcagatc acgaaacaaa ataatttttg aacttgatag aaagagaaaa
     4801 gtagcaagag aaggggataa gcaattttct tgtcactttt ctctttttct gatttgttat
     4861 tatttgtagg aaaaaagata gaaagcaaaa gacttttttc gattaaagcg ctattggata
     4921 ttttattttt tgcaaaaaac ttcttgaaaa ttttcatttt cattgatttt agtagaaaac
     4981 taccgaaatg tttggtaaaa tagggtcgtt ggttaagaat tgtcttaaca aatttagttt
     5041 tacaaaactt aggaggaatt ttattatgcc agtagtatca ggagctgaat ttttaaaagc
     5101 tgctcgtaaa ggcggttacg ctgtcggcgg attcaacaca aacaacttgg aatggactca
     5161 agcaatcctt gaagcggctg aagcaaaaca agcaccagta cttatccaaa catcaatggg
     5221 tgcagctaaa tacatgggtg gttacaaaat ctgtaaagat ttaatcactg atttagttga
     5281 agcaatgaac atcactgttc cagttgctat ccacttagac catggtgatt acgaagctgc
     5341 tttggaatgt atcgaattag gctatacttc agtaatgttc gatggttcac atctaccatt
     5401 tgaagaaaac ttgaaattag ctaaagacgt tgttgaaaaa gcacatgcaa aaggtatctc
     5461 agttgaatgt gaagttggtt caatcggcgg agaagaagac ggaatcatcg gtaacggcga
     5521 attagctgat attgaagaat gcaaacaaat ggttgctaca ggtatcgact acttagcttg
     5581 cggtatcggt aacatccatg gtcaatatcc agaaaactgg aaaggattag cttttgacca
     5641 cttacaagca attgctgatg ctgtaggtcc agacatgcct ttagtattac atggcggatc
     5701 aggtattcct caagatcaaa tcgaaaaagc tatctcaatg ggtatctcaa aagtaaatgt
     5761 taacactgaa ttccaattat catttgctgc agctactcgt aaatatatcg aagctggtaa
     5821 agataaagaa ggtaaaggat tcgaccctcg taaattatta gctccaggta aagcagcaat
     5881 cgttgaagat gctaaatctc atatcgactg gttcggttca gctaacaaag cgtaagcatc
     5941 cacataacag atacgatata taggaatgga gaacgacttt tgaagtcgct ctccattctt
     6001 ttttacttaa aacggcagac catgctcgtt tctttttttt atctatgata aaatacaagc
     6061 atatccatga aaaaagatta ataaacaagg tggaaacaga tgaaaaaaat cgtaatcaaa
     6121 ggaaaccgac ctttagctgg tgaagtaacg atcagcggtg caaaaaatag tgcagtcgca
     6181 ttgattccag ctgcaatctt agctgattcc ccagtgatct tggaaggtgt gccggacata
     6241 caagatgttc attcattaat tgaaatttta gagatcatgg gcgcaactgt ccatttttcc
     6301 aacaacatac tagaaatcga tcctcgagaa gtcgtatcga ttccgatgcc gcacggaaaa
     6361 atcaatagtt tgcgtgcttc ttattatttt atgggaacac tgcttggtaa atttggcgaa
     6421 gcggtagtag gcctgccagg tggctgttat ttaggcccta gaccgattga cctacacgtc
     6481 aaaggatttg aagctctagg tgctgaagtg acaaatgaac atggtgccat gtatttacgg
     6541 acagaaaata aaggattgcg tggcaatcgg attttcatgg acgtggtttc agtcggagca
     6601 acgatcaatg tgatgttagc tgctgtaaaa gcaaaaggac agacagtaat tgaaaacgct
     6661 gcacgagaac cggaaatcat agatgtggca actttactga ataatatggg ggcaaaagtt
     6721 cgaggagcgg gtacagatgt gatccgtatc gaaggagtcg aaacattaca tggctgtcgg
     6781 catttcatga ttcctgaccg catcgaagca ggaacctatc tagcattagc agctgcagta
     6841 ggaaatggta tcaaagtgaa aaatgtgatc tttgaacatt tggaaagctt tatagcaaaa
     6901 ttgcaggaaa ttggtgtcca tatgaaaatc agcgaagatg aaatcgaagt ttacccctcg
     6961 aaagaattaa aacccgcaaa catcatgacc tatccttatc caggatttgc cacagatctg
     7021 cagcagccac ttactgcgtt actattgatg actacaggca cttcagaaat catcgatacg
     7081 atctatgcga aacgtgtgaa ccatgtgccg gaactagcac gaatgggcgc tgatattacc
     7141 gtggaaggaa atatgatcat cgtgaatggt ccaaataaac ttcacggcac agaagtcgtg
     7201 gcttctgatc ttcgggcagg tgcttgttta gtgattgctg gactattggc agaagggaca
     7261 acgacgattt acaatgtaga ttatatctta cgcggttatg atcatattat cgagaaacta
     7321 acagccttag gggcatcaat tgaaatgata gaggaagtgg gagctgaatg agcgattatt
     7381 tgacaatggc agaactagag aacaaaacgt taaaagaagt ttatagttat gccaaagaat
     7441 ttaaaattcc ttattacagt aagatgaata aaaaggaatt atctctagct gtgattcgtg
     7501 cacaagcaga gaaacaagga ttttattata tggaaggaat attggatatc gttggtcaag
     7561 atggttacgg ttttttacgg ccaatcaatt atggaccaag tgcagaagat atctatattt
     7621 ctgcctcaca aatcagacga ttcagtttga gaaatgggga caaagtggca ggaaaagccc
     7681 gtccacctaa agaatcggaa cgttattatg gtttgatgca cgtggaaagc gttaatggaa
     7741 aagatccgga agaagcaaaa gaacgtccgc attttccagc cttaacacct ctttatccgc
     7801 aaagacagct tcaactggaa acaacaccgc aaagactatc tacccgaatg atcgatatct
     7861 tttctccaat cggttttggt cagcgtggat tgatcgtcgc accgccaaaa gcagggaaaa
     7921 cttctgtatt aaaagaaatc gcaaatggta tcaccgaaaa ctatccggaa gtagaattaa
     7981 tcgttctact gattgatgaa cgaccggaag aagtaacgga tctagaacga agcgtgaaag
     8041 gcgacgtggt atcttctaca tttgatctgc agccgcaaaa ccatactcga gtttctgaat
     8101 tagtattgga acgcgcaatg cgtttagtcg aagataaacg agatgtcgtt atcttgatgg
     8161 atagtattac tagacttgct cgggcctata acttagtcgt tccaccaagc ggacgtacat
     8221 tgagtggcgg gattgaccca gccgcattgt ataaacctaa gaaatttttc ggggcagcaa
     8281 gaaacatcga agaaggtggc agtttgacga ttttagcgac cgctttagtc gatacaggaa
     8341 gccgtatgga tgacgtgatc tatgaagaat tcaaaggaac tggaaatcta gaattacaac
     8401 tttcacgtga attatctgaa cgtcggatct tcccagccat cgatatcaaa aaatcaggta
     8461 cccgaaaaga agaattattg atgccaagtg aagaattaga agaaatttgg aaacttcgaa
     8521 aaaatatgac tggagattca ttagaatata cggaacaact gatccgcttt ttgcgtaaaa
     8581 caaaaaacaa tcagcaattt ttcaaagaat ttcaagatgt ctcctttggt aaaaaaaatc
     8641 cgaccagaaa tctgaaaaga taattgcaac cttgactgaa acatgttaag atgttaatgt
     8701 tgtaaatcga caaatcaact ctggttcagc aaatggagca gggcaaagga gcgaaaaaat
     8761 catgaaagaa aacatccatc cagattacca cccagtagta ttcatggatt caacaactgg
     8821 tttcaaattc ttgtcaggtt caacaaaaac ttcacaagaa acagttgaat gggaagacgg
     8881 taatacatac ccagtcatcc gtgtcgaagt aacttctgac tcacatccat tctacactgg
     8941 acgtcaaaaa ttcacacaag cagacggacg tgtggaccgt ttcaacaaaa aatacggtct
     9001 caaagacgca aacgctgcgg aataataaaa acgctgataa ctcaacgttt agcagactat
     9061 ctgataggga tagtctgttt ttttatgttt tttgccaatt taaaacttcg tttgatgctg
     9121 aaaaagtcgt aggatcataa tgcacaaaac aatgaaaaac tagtatatag ttttaattat
     9181 ccacttgata aaaacacctt atattaacga aatgaagagg ggttatttcg aagtaaaaaa
     9241 ccatttacct aaaattagaa gctaagcttt cctttcaagg aaggcttttt gttttgttat
     9301 agacttgcga ttgtttgtga gctagatcgt tcattttaat gacttataca cgcggatgat
     9361 gggtcttttt cctctgttca ttcataatat tgaacggtac agctagcgat gctatactga
     9421 cctcagttcc aagaaaacgt taacattttt tgtgggggag aggtttttta tgaggtttga
     9481 tcaattgatc gcagaagaac tgattgtttt cgatccatcg atagattcaa aaggatcatt
     9541 atttgagcga ataggggatg tgttgaaaaa aacaaataag gtaagaaatg ttcaaaaagt
     9601 cgttaaggca ctattcaaac gagaggagga gatctccaca ggaatagaag atggatttgg
     9661 gattccccat gcaaaaagca aatatgtgac aaaaccagca ctgtgttttt ttcatacagc
     9721 caaattaacg gactatagcg ggatagatga aatgccaatt gaatatattt ttgccatcgt
     9781 agttcctgaa aaatcagcag atatgcatct agagattctc agcacactct ccagacgatt
     9841 gatgaatgca gattttagaa agggaataaa agaagccact acgccagcag atatcatcag
     9901 acagattagt caagaatgaa aatagtagag tctttgtccg agtatcgata gtgaagaaaa
     9961 aggaggaaaa taatcatgaa aatcgttggt gtaactgctt gcccgactgg aattgctcat
    10021 acttatatgt cggcagaaaa attagaagta acagcaagaa atctaaacca tcaagcaaaa
    10081 ttcgaaacac aaggtgtaaa aacagaaaat cagttatctg aacaagaaat caaagaggca
    10141 gatgcaatca ttcttgcagt agataaagaa atcgaattgg accgatttgc cgggaaaaaa
    10201 gtaaagcgtg tctctacaag ccgagcaatc aaagaaccgc aagtagtaat agacgaagca
    10261 ttgagagata ttggcgtatt cgttgtgtct aatgaaaaag aaccagctgc aaatgagaaa
    10321 aagccgacaa tctataatca tttcatgaat ggtgtgaatt acatgcttcc atttgtgatc
    10381 gcaggcggaa tcatcatcgc actgagtttt gcctttggga ttacagcggc tgatcccaac
    10441 tcagcagact ataatgtgtt agcagctgca ttttcaagga tcggaggaga tacagcgttt
    10501 gccatgatgg ttcctgcgtt aggcgcgggg attgctacat cgattgctgg aaaagcaggt
    10561 tttgccccag gaattgttgc cggattactg gcctctacag gtggttcagg ttttcttggc
    10621 ggaatgattg gtggcgtgct agcagggtat atctgtgatt ttctggcaaa taaaattagc
    10681 gtaaaaaaag aattttctgc tatttatcag ctgattgtcg tccccttttt atcgatctta
    10741 attattgggc ttttgatggt atttgtgatt gaacaaccga tcacatgggt gttggatgct
    10801 ttgacaaatt ggctgaatag cttgggaaat acatccgggc tattgttcgg attacttatc
    10861 gggatcatga tggcagctga tatgggtggg ccgatcaata aatcgacttc gactttctcg
    10921 atcggtttga tgtcagctgg agtaaatgca ccgattgctg cttgtatggc tgcaggaatg
    10981 acacctcccc taggtattgc tttagccact ttattattca aacataaatt taccaaagaa
    11041 gaacggactt caggtcaatc atgctggatt ttaggcgctt cgtatattac agaaggtgct
    11101 ataccgtttg cggtatctga cccgatccgc gtgataccta gtttgatgac aggttcagca
    11161 gtcgctgcag ggatttccat ggcagtagga gttacttcaa tggcacctca tggagggatt
    11221 tgggtgatgc taattccaaa tgttatgaac aatttaccta tgtatgtctt ggcaattgca
    11281 tcaggtacag tcactacagc ctttatgctt ggactattga aaaaaacagt gaaagagaaa
    11341 aaacaagaca agtggacgaa aaatgaaaat ctaacaaatg aggtggaagc gtaatgttat
    11401 atacaatgaa agagttattg aaagtggcta aagagaatca atttgcagta ccagccttca
    11461 atatttgcag ctatgatatg atgaaagcaa tcatcgaaga agcagagaga ttagaagcac
    11521 cagtaatctt agaaattcat cctgatgaaa tcgcctattt aagtgatgag tttgttgcgg
    11581 cagtaagagc ctatgcttat acaagtaaac tgccgatcgt tatccacttg gatcatggcg
    11641 gtacgataga agatgtctta cgggcgattc gaaacggcta ttcttccgtg atgatcgatg
    11701 cgtctttgaa atcattcgac gacaatgtac agattacgaa aaaagtggtt gcgttagctc
    11761 atgcggtaaa tgtctcagtg gaggctgagc ttggaacgat tgggaataat ggttctgctg
    11821 aaggaggtgc tgaaacaatc atttatacag atcctgatca agcgaagatt tttgtgaaag
    11881 aaacaggaat cgatacactt gctgtagcaa ttggaacagc gcatggcttg tatccgaaaa
    11941 acagaactcc aaaattaaat atcgacttac tgaaagaatt gaaccaacaa atcgatcttc
    12001 catttgtttt acatggcggt tcaggtaatc cggacgcaga agtcagcgaa gcagtaaaat
    12061 acggagttgc caaagtgaat ttgagctcag atcttaaaag cgtctttttc aatgctttga
    12121 gagaaatatt gagtgaaaat ccatcgatgt acgagccgaa tgcaatttat ccagcagcta
    12181 ataaaaaggt acaagaagtg gtcaaacata aaatggaaat tttgaacaca gtaggcaagg
    12241 ctagtttata ctaaaaataa aaatgtgtgg tcaagagggt atgacaatag tcttgtcata
    12301 ccctcttaac cacacacgat gttcagaagt agttttttcg aaaaatttct tcttatttct
    12361 cgaagccaat cacttctgtc acaacctctt ttttcctgtt actatataaa gtaaatgggg
    12421 tgattttatg ttgaatgaag tggagaatag acagcaggag attgtgaatt atctaaaaaa
    12481 caaccaattt gcaagagtca ccgatttgat cgagttagta aattatagcg aagccacgat
    12541 taaacgtgac cttgtttttc tggaaaaaag tggattgatc cgtagagttc gcggtggcgc
    12601 catgcttatt gacaatcgaa aaattgacgt gccttacctg atgaagatca ccaaattaga
    12661 agaagaaaca gaaaaacgta agattgcaga tacagcttct tctttgattc gagatgatat
    12721 ggtcttattc ttggattcca gtacaacctc tttacactta gtcagaaacc tagcaagatt
    12781 tgatggtttg cagatcatta cgaatggcat cgtcacagct gctatgttga gtgaatatac
    12841 aacggcaaaa gtatctgtat tgggaggggc aattgttccg aaacgagcaa caatcaacgg
    12901 ttcaaaagcc tataacgatg cattgtcata ttatgcggat ttagcgataa tgagttgtcg
    12961 cgggctagat ttcagccaag gagtaacaga aactcatgaa ggagaggcaa tggtcaaaaa
    13021 agcttttaga aaacaatcag accggcttgc cgttttagtt acacagcaaa aaattgcaca
    13081 taaattcatg catcaagcca ttgcttttca tgaaatcgat tatctggtga caagtaaaga
    13141 actgacaaaa gaacagtatg cgaaagtaaa ggaacaccat atcgaatgtc tgtacggata
    13201 aattattacg tgaatgttct aagtggcttc ctcattttta gataggacct tatctttgct
    13261 cgcttctctc agccgatggt ataataatga aaaggttatc ttgttagata atcttacttt
    13321 tttatccagg agaaaggagt gcagaaaatg aaacatgtga aggcgttggt agtgaaggca
    13381 atcatgatct gggcgattct gtgggtcgtg ctgacaggtt tatacggtgt tagctttatg
    13441 gattcaacga ttgttggggt aattattgta gtcatgatct atgtgctagg ggatctgttg
    13501 atcttacgta aagtagggaa catcgcagcg acgatcgcag atgctggttc agctgcagtt
    13561 atcttgtggc tctatctata ttttatgaat gatacagtcg atatttggat gaaagtattg
    13621 attcctgctc tattgatcgg tgtggcagaa tggttcttcc acaagtggtt gctaagtcaa
    13681 ggcatcgtac cagatgaacg gaaaatggaa taaatcaaca gttaaactat tcttatcgat
    13741 aaatgaaagc tttaaaaata aatagatgtt taattaagaa taatcataaa ctatttttac
    13801 tcaaaaaacc gctcagatta ttttcagatc tgaacggttt tttttatcta ttcatcaaga
    13861 tcaaatttca tttttcaatg attcaatcaa ttcgccctct gcttcatgac tatgttttcg
    13921 gccattaaca agatagagtg ctgtacatat caatggaatg actccggcaa tcacatacaa
    13981 tcctctaaat gaagtaagag attttagcat ccccatcaaa taaggaccaa cgcctaagcc
    14041 taggtctaaa ccaataaagt aggtagataa agcaatactg attcgatgtt cgcggacgat
    14101 ttttaaacaa acagcctgtc catttgacat aaacgtacca taacctaggc cgactaatgc
    14161 tccagagata agcaatgtca cactattcgt cgttacactc agtaaaaata atcctgctgt
    14221 taagaataaa tagctaggat acatcacgta ttgttctcct ttgacatcga aaatccttcc
    14281 agtcaatgga cgtgtaaatg tgatgaccaa agcatatacg acgaagaaaa atgacccagc
    14341 agcaactaag tggattactt ttgtataaga agataaaaat gataaaacac ttgaataaga
    14401 aagacccatc aaaaaggcaa ttcctgtaat gaaaagtgct ttcttttcaa taaaactatt
    14461 gatattccat gagcgaagag atattttttg ttcttcagat aatggaatat tttttacagg
    14521 aaagacaagg caggcaatcg taacaaagaa gaccagtaca attgaaaacc agataatgaa
    14581 acgaaaatca gtcatattca gcaaaatcat tcctaaaaat ggcccaacag cggcagctaa
    14641 actagtactt aatccataat aattgatgcc ttcccctttt ttagatgcag ggatatatgc
    14701 agtaacgata gcatttgtag ctgtagaaac tgtaccgtag ccgaatccat tcaagaatcg
    14761 aactaaatac aatgccccga tagttggcgt ataaagatat gctaatgttg aaaggagata
    14821 gaagatcgcg ccgcctctta aggtaaactt ccgaccaaat agttctaact gtttccccat
    14881 gaataagcgt gccagcaatg ttccgatgat atagatccca gaagcaaaac ctgcttggct
    14941 caagctggcg tgtaaggtgt cttgcgcaat gactgccatg atgaccatca acagataata
    15001 aatcaggtag acaataaaat tgatcaatgt gattccaata aaatatttat taaataattt
    15061 cttttccatg tatgttatac attcccctta agctgaatat gcttttaatt tttgtatttg
    15121 aacttctaca tcgtgataat tggatggata gcgatcaaca agttggcgaa taaataaatc
    15181 tcttgtagac ataaacagat ttaaaaattc ttcgcattcg tttgttggat tctcatccac
    15241 tgctttgatc aattcgatcg agttttgata cgcatcattt aaaaagacta aagaagcttt
    15301 catatcaaat cttgtttgat atgctgtttc taatcgatta atgattgtgt tcatccagta
    15361 attcatgttt tttccctcta ttcttaaaaa attttacttc tgcagtataa cgaataaaaa
    15421 aaaggaaaaa aatcgcataa gtgatggaaa aaggtactta acaaatgtta atatcattag
    15481 taaaatacct ttggacttta aaaagtttta aagtactgta tgatcttttt agatgaaagt
    15541 ttggatgggg gagcatcatt ggatagtaga gtcttaaagg aatatttgga taaaaatgat
    15601 tttccgatta tacgcaaagg atatcataaa tatttgacat tcgaagggat ggaagattcc
    15661 catacgtata tcttgaaaga gggaacggta aaagcaagtg ttatttcaaa tgatgggaga
    15721 gaatttaacc ttcgctatat caaagatctt gaaatcgtct ctcttttaaa agacgaatat
    15781 tcacaattca tcgattcgcc gtataatatt cgagttgaat cagaatatgc agagttttat
    15841 cgaatcaatc gtgtgcgctt ttggcaaaat atcaaccaag acccaaagct acaacactat
    15901 gtaaaagagt attaccgcta tcgtctgctt tattcgatga aaaaaatgca gcaaatgcta
    15961 ttaaacggaa agctgggagc tgtttgtacg caactttacg aattatatga tttattcggt
    16021 gtgcggacag aagaagggat gctgatcgat ttttcgatca cgaatgaaga aatcgccaaa
    16081 ttttgtggca tctcaactgc tagcagtgta aacagaattt taaaacaact gaaaaaagat
    16141 ggtattatca ccgttaaaga ccatcatatc gttattttga atatggactt gttagaagac
    16201 aatattatct tttgatgaac aaagaggttg tgaaatcagc gttttgacct gagtattaga
    16261 gaaacaaacg gaaaaatagt tcctcatatt ttgccgtttt ttgagctaat accgagggtc
    16321 agctgattga acaccgttta ccaagaggtt gtgagcctgc cgtttaactc tgagtcacaa
    16381 ggaacaattt caaaaaacag cttttcatgt ttgatgaata ttgtgacttg tgaccgagga
    16441 gttggtagga gaacaccgtt taccaagagg ttgtgaaaaa gctgttctgc atcaagtaat
    16501 aagaagagcc aagtaaaaac agtctcttat gtttttaact tggctggact tatcaccgta
    16561 gatgtaagct tttgaacgcc gtttatttgc aacaaaaggg gctgtgacac gatttttgtc
    16621 acagcccttt ttttgcgaac attcatttag caatttttag aaacttctgc cgccaccgcc
    16681 aaaagtcccg ccaccgctag aatgggtagt acttccgcca ccgccgcttc caggaggtgg
    16741 gcttttgggg attcttcttg tcgtgacaaa agaatttgtc agacgatcgg ttttttcagt
    16801 taatttgata ctggattttt ctcggaatgg atatttgtag atgcctgatt tcaattgata
    16861 acgcgaaaca gtcacaaaat aaaagacaag actaaggata agagccaaaa caacagcaat
    16921 gatgatttct actgtcgtca aaaccttata gcgtgtaatt ttgccagttt cttcgtctac
    16981 acgataatgt cctcctggaa ctccgtcttc cacatatgtt gctgatttag acaggtaagc
    17041 ttttgcagca ttgtaatagg cagcatttgt catctgatca taaacatcat ctaagatcga
    17101 atcgattctg ctatcagtca aataatcgat catattacca gaagtcgaaa tgtagatttc
    17161 acgctgtccc atatctaata gcaatactga gccattttga tcatttccga ctgcgtctct
    17221 taagtaatta tcagcaaagt ctcttggttc ttctgaatta gatgtagtcg ttacgatcaa
    17281 gactcgtcct ttgatttttt cattcagctt ttctgcctgt tgttccaaag attggatttg
    17341 atctgttgtg aataacccag cctcatcatt taccgtagct gtatctgcat gagtagccac
    17401 cggccagcca acacttacta gcaaaagaaa gaataaaaaa aattttttca tcatatcaag
    17461 taacctccga tcagaaagag gatcgctagc ccagcgaaaa tcccaagagc ggtcagtccc
    17521 aattttttct ggctgatcgg caggacaccg ctgacctttc ctgtttgtcc attcattgcg
    17581 tagtagtaga cttttttact tgagtcattg cttcgataag taacaagcca taccggcagt
    17641 aatacgtaat tatttacttc gtttgtgatt gaagcagaag ttcgcacacc ggtcaatgtt
    17701 gtatatccat ttgcggtatc ttttaacagc ttctctgaat agtcttttaa ttcagattga
    17761 atctgtgatt tgattgcctc atattcgata tctctttttt ctgcttggaa cccggctaaa
    17821 tactggcttt taaaatcaat tgctttatct aacgggaaag gttgcacgcc ttcgaccatc
    17881 ttttgttgcg tattttttga taaggcattt tttactagtt ctttaaaaga aagcttgcct
    17941 gctcttgaaa cagcaaattg cttcgtttct gtgtattcga tgtcacctat ccgccatatg
    18001 cggataactg taccattcgc ctgcatggca ccatctactt cagcatctgt agcccaataa
    18061 gggaaataca cacctgtcaa cttatcgatt tgatccttat tgaaaaaagc tttgggaaca
    18121 aaccattttt tcttggtcca agcgagaaat ctgttgattg cttcttcttt ctcgacagca
    18181 aaaggaagta ctttatttgg caagaattct ccgcttaaac gtcccgatag tacgacggga
    18241 ttatggcagt agtaacaata agtcgcagca gttgtcgcat cagtcacgat ctctgcacca
    18301 cagttgggac aaagaaaaag atctatcgct gcggtattat ctgcagattc gtccgctgta
    18361 tctgaaaaac cgcctgcctc gtcaaccgct ttgcgctcgg cttcattcat atcggcaagc
    18421 tgttcttggg ctgtaaaagt aaaatcttcg gtaggtgaag gttcttcttt gctttcttcg
    18481 ccagccatac gcgcttcttt ttgcttttgc tcatattggc tgacttcttc ttctgtatag
    18541 atattcaggc agtattcgca atgaaatttt tggtctttgg gatcaaaagt caacggaccg
    18601 ccgcaattag gacatttatg tgtaagaaca tccatcagct gcacaccttt ctaaataaat
    18661 taattaacgg ggattccttt aaacggttta ccacagtttg gacagaattt agggatgccg
    18721 ttggaaagat ccacgatctc attgcattca ctgcatcgca tttgtagctt aggctgaacg
    18781 ttttctacag gtttggcagc tccgcagttg gaacagaatt ttccggaatt ttctgtcccg
    18841 caaactggac atgtccaagt atccgcattt ccttgtgcag tttgttgatt ttgtttttct
    18901 gcttgttgtt tgatttgttc ttggttattt tgagctgctt gagaaagata agaaccattt
    18961 gcattcattc ccatacctac acccataaag ccggtcattg ctcctgcttc attttttccg
    19021 gctgcttcca taccacgtgc aatggaacct tgcacgtatc cttcacgtac gctaggatca
    19081 cctagcatcg ccccttcgtt acgcatattg atcagtttca cagaatcatc ggtgtaagaa
    19141 atgctggcta cagccacaga aacaatctcc atcccacgta attcacgcca agaatcatcc
    19201 aatgcagtat ccatgtattt gcttaattcc aaactcttag atggtacata agaaatccgt
    19261 tgaccatctg cagacatctg attgattgct gattgtagcg ctgttaggaa ttcagctaaa
    19321 tactgctcat tgatatcatt gatttccact tgtgttttgt ttttagggat tgcgtttgta
    19381 taaaataaaa tcgggtcaac gacatgaata gaataagtgc catgtgcccg taagaataat
    19441 tctgcgttat agaaattgtc aaagtagttc aacggcgcgc ttgtaccgaa tttgattccc
    19501 tttatttctt ggagattgat ataaaaaact tgttgtttct gcggtgtgac gccgccaaat
    19561 ttgaatcggt caaaagtttc agcaattgct tctttcagtg ttccattgaa catggaagga
    19621 gctgcgtcat ttttaatggt gtaataacct tcctcagcgg tataatcgat gatttttcca
    19681 ccatcgacca aaagcatcat catgtttgga tagacatgga cgactgttcc gtctgttaaa
    19741 acatcttctg ttcctttacg attggagcca cgtttgtcat ctttgcggac tttgacacct
    19801 tttgtcatga ccgttgtatc actcatattg tctggttcga tgacttctag ccattgatct
    19861 gccagaccgc cgccaaccat acttgttgct gctttgataa gacccataaa aaattcctcc
    19921 tctgattcag ctagtgccaa acaattattg tttatttatt ataccatagg aagatgtgaa
    19981 acttctcgga ctagagacgg aattgaaatg gaaaattgcg tattgtttaa atacctgaaa
    20041 aagccgagaa ggatagtagt ttttttacct aatatcacgt ataataagag atagtgaatt
    20101 ttttaaaaaa gggggatcct tatggcatat caagcacttt atcgagtctg gcgttcgcag
    20161 cgtttcgaag acatcgttgg acaaaaagct gtgacacaga cactaaaaaa tgctattgtt
    20221 tcccacaaaa cctcacatgc ctacttgttt acaggaccca gaggaaccgg aaagacgagt
    20281 gcagcaaaga ttttcgcaaa agcaatcaac tgtccgaaca gtaaagacgg tgagccatgc
    20341 aacgaatgcg aaatgtgtcg atcaattact gctggtacgc aagaagatgt gatcgaaatc
    20401 gatgctgcca gcaataacgg agtcgaagaa atccgattta tccgtgatcg ggcaaattat
    20461 gcgcctacaa aggcagcgta caaagtgtac attattgatg aggtacatat gctttcaaca
    20521 ggagctttca atgccttgtt gaaaacattg gaagagccaa aagaaagtgt catattcatc
    20581 ttagcaacga ccgaacctca caaaatacca gctacgatca tttcacggac tcagcgattt
    20641 gattttaaac ggatcaatac ggcagatatt gtcgaacatc ttgaatttat cttgaaagaa
    20701 tcgactatca gctatgaacc agctgcattg aatgtgattg cccgttctgc agaaggcggg
    20761 atgcgggatg cattgagtat tcttgatcag gcaatctcgt ttagtgaagg aacaattact
    20821 ttagatgatg ccatgcaagt gacaggtagc ttaactgacg aaatgatgga cagttacttg
    20881 agtgcttgcg tgaaaaaaga agtaccagaa gcattggaaa cattgaacca aattctcgca
    20941 gccggcaaag aatcacgtcg ctttctagag gatatgttac aatactgccg tgacttgctg
    21001 atgtatcagc aagcacctaa cctagttgag gaacaatcga gtacgatcag aagtacattt
    21061 acagaattgg cacaaactgt ttcagcggat cagctgtttt caatgataaa gattttgagc
    21121 gatactcaaa atgaaattcg ttttaccaat agtgccgcta tttatttgga agtcgctaca
    21181 gtcaaacttg cacagctacc gtctgcttct gttccttcag cagatgtagc atttgcagat
    21241 gatcagatag taaaacagct acaacaagag attaaggaac taagaaacga aatcaaggaa
    21301 ctacgcagta atacaggaat tgagaataca aaagtacagc cggtacagca aacaaatcat
    21361 aaaaaacaga caagtaccta tcgtgtaccg acggaacgag tatataaagt tctgcgggaa
    21421 gcgacaagac agcatctgaa ccaagtaaaa gaagtatggg aagacttgct gatgagtcta
    21481 tctgtcactc agcgagcaat gcttaaagca agcgaaccag ttgcagctgg acctacagga
    21541 ctagtgattg cgtttgatta tgagattgtt tgtcaaagag cagcaaatga cgaagaattg
    21601 cagctttcgg tacacaacaa cttaagccga atgatcaaag attatgcgcc tgatcctgtt
    21661 tatattacaa gagaaagctg gccagaactt cgaagaaatt atttgacgca ggcacaagaa
    21721 gagggaacag aaaactcttt tgcttctgaa gactcagaag gagaaatcga gttgcttcct
    21781 caagaacaag aagtaccaga agaaacactt gtagtaaaag cagaggaact gtttggctcc
    21841 gaagctgtta cagtggtaga tgattaataa aaaggagtag ataattatga tgcgtggaat
    21901 gggcaatatg caaggcatga tgaaacaagt acaaaaaatg caaaaagaaa tggcagaagc
    21961 acaagagctg ctaaataaaa cagagtttac aggtaaagcc accaatgaat tggtcaaagc
    22021 ggtttttaca ggcgatcgtc gcatgaaaga tatccaaatc aatgaagaag tcgttgatcc
    22081 tgatgatatc gagatgcttc aggatcttgt cgtaatggca gtcaatgatg cgatcgcaca
    22141 aattgatgtg gaaactgaga aaaaattagg gaaatacgca aaaggtttgc caggatttta
    22201 gttttaaact gaaggagtaa tgaatatgca atatccagaa ccaattgcga aactgatcga
    22261 cagctacatg aaacttccag gaatcggtca gaaaacagcg acaagactcg ccttttatac
    22321 gatcgatatg aaagatgaag tagtaaatga atttgctaaa tctttgttaa gtgtaaaacg
    22381 cgatttgcat ttttgtagta tctgcggaaa catcacagaa gaagatccct gcgaaatatg
    22441 tagaaatcct tcacgtgatc gaagcgttat cctagtggtc gaagagccga aagatgtaat
    22501 ggcaatggaa aaaatgcgag aatatcacgg actttatcat gttctccatg gtgtgttatc
    22561 accgatggaa gggacaggtc cagaagacat caatatttct tctctgttgc agcggctgca
    22621 tgatgcagat gtaaaagaag tgattatcgc aacaaatgca acaacagagg gagaagcaac
    22681 agctatgtat ctctcacgtc tgatcaaacc cgcaggtatc aaagtgactc gtctagcaca
    22741 tggtctgtct gtaggctctg atatcgagta tgcagatgaa gttactttat tgaaagctgt
    22801 cgaaggaaga cgtgagttat aaatttctaa ttaagtgttt tttcagatac aatataaaag
    22861 cagcacaaga gatgggggca acaatgtgaa cggactattt attacaatcg aggggcctga
    22921 tggtgcagga aaaaccagca tcttgaatga actttaccct cttttgaaag aagtagcaaa
    22981 aacagaaatc attcaaacaa gagaaccagg aggcattccg attgctgaag agatccgagc
    23041 agtgatcctt gatccaaaaa atgatcggat ggatgaacgt acggaagcac tgctttatgc
    23101 agcagcaaga agacaacatc tagtcgaaaa ggttctgcct gctctggcaa aagggaaaat
    23161 cgttttatgc gatagatttg tcgacagttc tcttgcttat caaggtgcag gccgacgcat
    23221 cggcgttacc gagattgctc ggttgaatga gtttgctaca gaaggtacta cgccggattt
    23281 taccctttat cttgacgtag attcagacac cggattaaga agaatcaaga aaaacagaca
    23341 aaatcaaatc gaccgtttag attcggaagg tttggaattc caccagcgtg tacgtcatgc
    23401 ttacttaaag ctggcagaag agaatccgga aagaatccac aaagttgatg caagaaaaag
    23461 ctttgaagaa gtgctgcaaa caagttatca tacaattatt gaacagtatc cgcaattttt
    23521 tgaaaactag gaaggtgaac gtttatgaaa attattttag cgattatcca agacaaagac
    23581 agcaaccgtt tatcaaatga attgattgat gcaaatatcc gtgccacaaa actatcatcg
    23641 acaggcggct tccttaaggc tggaaacagt acgtttatcg tgggtattga agatgaccgt
    23701 gtagaagaag cattggaaat catcaaaaaa acgtgtgaat caagaaagca atttgtatct
    23761 acacctgtta cactggatat ttcgatggat gggggcgtcc cttatccagt agaagtagaa
    23821 gtaggcggtg ccacagtctt cgtcttacct gttgaaggat tccatcaatt ttaggaggag
    23881 ctgagctatg aatcaagaaa taaacttggc tcaaatgcaa ccgatcgtgt atcagcagct
    23941 tcaacggagt tttgagcatg ggcgtatcgc tcatgcttat ctttttgaag gggagaaagg
    24001 aacaggaaaa catgaagtag gtatatggct tgcacagcac ctgttctgta cccaaatgaa
    24061 agacaatctg ccttgtggaa aatgcaacaa ttgtcagcgg atcgaaaaga aagaacatcc
    24121 agatgttttg gttattgaac cggaaggaca aaccatcaaa gtcgatcaga tcagacgttt
    24181 gcagacggaa ttcagtcgca gcggttacga atcaagaaaa aaagtttttt tgatcaaaga
    24241 agctgaaaaa atgaatagca gtgcggcaaa cagtttgttg aaattcttgg aagagccccc
    24301 tggagatttt ttagcggtgt tagaaacaga tgcagtagga agaattctcc ctactattca
    24361 atctcgttgt caagttctgc attttcaaga actttcgaaa gaagcactga tccatcgatt
    24421 gcaagaagag cagattccat tggaaaaagc aaaacttttg gctttcttaa caaacagttt
    24481 aggaaaagca gttgaaatct ctcaagatga atggtttaat gatgctaagg acttgattta
    24541 tcaatggttc gtttacttgc aaaagcaaga tactcaagcc tttatctatg tccaaaaaaa
    24601 attggtaaaa tcatttaaag aaaaaacaca acagtttaca ggattatctg tcttgatgtt
    24661 ttattatcag gaagcacagc gtcaagcagt agcaaacggc gaatatggca aagtgaaaca
    24721 gataaaccag ataattgaac ggatattgat cgcagagcag aaactacgca gcaatgtcag
    24781 ttttcaggct gttgcggaac agttcgtttt gcatacgatt ttttagcttg ccgcaaagga
    24841 tgatgatcaa ggaggatgga aatggtagaa gtagtaggtg tccgatatag agatgcaggt
    24901 catatctatt attttgcacc aggagactct gaatatgttt atagtgacaa ggtattagtc
    24961 gaatctcagc aatccttaca tattgctact gttgtccttc ctaaaaaaga gattgatccc
    25021 aatgaccttc ctgaagctat ccgtccgatc ttgcataaag cgtcacaagc tgaattagaa
    25081 aaagacaaaa aaaacaaaaa agatgcttat gatgcattga aaatcgcaaa agaaaaagtg
    25141 cagttgcgtg agttggaaat gaaaatggtc aaagccgaat acacatttga tcgaaataaa
    25201 ttgatttttt ctttcacagc ggacgggcga attgacttta gagaattggt aaaagatctg
    25261 gccgctgtat ttcgtactcg tattgagctt cgacaaattg gtgtccgcga tgaagcaaag
    25321 ataataggcg gaattggtcc atgtgggcgt ccattatgct gttcttcttt tttaggcgat
    25381 ttcgtgcctg tatcaatcaa aatggcaaaa gatcaaagtt tatctttgaa tccaacaaaa
    25441 atttccggtt tatgcggacg cttgatgtgc tgtctgcaat atgagagcga agaatacgag
    25501 acagcgaaaa aagaactccc tgactttgga aaagtaattg ctacacctga tggtaaaggg
    25561 aaagtcgtag ggttgaatct gcttagccgt atcgttagta ttcggctgac tggacgggaa
    25621 aatcctgtcg aatatgaatg ggatgaattg aaagaattta tccaagtagg tgagatgaat
    25681 ggataagaga tcattatacg acggattgaa tcaattagaa tcagagttga gaaatacatt
    25741 aacagaactt gttgaaatga agcatgcttt gcatgagatc gtagaaaaaa atacaacttt
    25801 ggagatggaa aatcaacgtc tgcgggaaca cctgcgggaa ttgaaccaag ttgcacaagc
    25861 tccagctgaa aatgtgaagc aggaactttc taagtctcgt atgaatcttg aaaaaattta
    25921 tgaagaagga ttccatgtct gctacgattt atatggatca agaagggaca atgacgagcc
    25981 ttgtgcgttt tgtctagaag tgatctatcg cgaaagcggt cgatgaccaa taacaagagg
    26041 agactggagc ggagaaatac tgctccagtc tttttattaa ggagattgtg acagatgtat
    26101 aatcaaaaaa gtttcaaggg aaatccggta tatggggatt tatatcttgt accgacgcca
    26161 attggaaatt tagaagatat gagctacaga agtgttcgta tgctgcaaga atccgatctt
    26221 atcgcaagtg aggatacgcg taatacacaa aagttattga atcattttga aatccataca
    26281 ccccaaaaaa gtttacatga acataattat aaagaacgtg tccctcaatt gattgctttg
    26341 ttaaaagaag gaaaaacgat tgctcaagtc agtgacgcag gaatgccttc catcagcgat
    26401 cctggacatg agctagtttt agcctgcata aaagaaggga tttctgtgat ctctatccca
    26461 ggacctacag ctgggatgac ggctttgatt gcttcagggc tagtgcctca accattttta
    26521 ttttatggct tccttccacg taagaaaaaa gaacagaaag aaacattgaa aaagctaaaa
    26581 gaacagacag ttacgctgat tttttatgag tcaccttatc ggatcactgc tacattgacg
    26641 aatttcttag aaatttttgg aaatcgccaa gtcgttttgt gtcgggaatt gacaaaggtc
    26701 catgaagaat accttagagg aagtacggaa gagctactcg cttatatcga agaacatcca
    26761 gtaaagggtg aatgttgtct gcttgtagaa gggaatttgt tcgaaatgcc agtgacggaa
    26821 gttgatgaag agttgggaac gttaaaggaa caagtagaag caaagatcgc ttcaggcgaa
    26881 aaaccaaatg ctgctatcaa agcagtagcg ctgaaaaatg ggctgaaaaa acaagaagtc
    26941 taccgtcagt accatgaatt agactgagat agtataagtt gtctataatg aaagactaat
    27001 gaggaaatca gctactaact tttcaaatct cttgaacgcg aacgaatgtt cttgtataat
    27061 gaataagagg tgaggatgat ggagctgcaa tttcctttac ataatgatac atcaagaaaa
    27121 atcattcata ttgatatgga tgcttttttc gcttcggttg aagaacgaga tcatcctgag
    27181 tttcgcggac atccattagt aattgccaga caccccagtg acactggtgg gcgtggagta
    27241 gtgactacag caaattatga agcaagaaaa ttcggaattc attcagcaat gagtgcacaa
    27301 aaagcgtatg agctttgtcc gcaagccatc tttaagcctg gcgaccatca taaatacagc
    27361 gatatttcca aacaagtacg ggaagtattc tatcgttata cagatattgt ggaaccagtt
    27421 agtatcgatg aagcgtatct tgatgtgacc gaaaataaaa tcaattgcaa gtcagccatc
    27481 aaaatcgccc gaatgatcca agcagatatt tgggagagtg tccagctaac atgttctgcg
    27541 ggagtaagtt acaataagtt tttggccaaa ttagcatcag actatcaaaa acctagagga
    27601 atcacggtag ttgcccctca agacgctgtg ccatttttaa aagctttgcc aattgaaaaa
    27661 tttcatgggg tagggaagaa aactgtacct agaatgcatg agctagggat ttatacagga
    27721 aaagacctat atgaatgcac agaaatgatg ttgattcgaa attttggaaa aatgggctat
    27781 tctctttatc gtaaagtcag aggaatccat gattctccag taaacgttac aagagagcga
    27841 aaatctgtgg gaaaagagca tacttatggg cagccgttgc aaacggaaga agcagtgctg
    27901 actcagttgc gccaactagc tgaaaaagta gaagaagcac tgagaagagt ccaaaaacat
    27961 gggaaaacag tagttttgaa ggtcaggtac acggactatt ctactgtgac caaaagagtg
    28021 acattgccag aatatatcta taaaaaagaa gcactctttt atcaagcaag ccttatatgg
    28081 gaggaaatct tgggagtaga aaaaggaatc cgattgttag ggattacttt gacgaatctg
    28141 gacccgatga cttatgaaaa catcgtcctt ccattatggg aaaatcaaga aatataaaaa
    28201 cagctttgag agttgaaacc ggaaatccgt tttctctccc aaagctgttt ttatatcaag
    28261 tcttgctttt tgatttgttc ataggtttct gttgcatccg tacatttttc ccaaatcaca
    28321 acttgattgg attctaaaga tttttggacg tcaatgatcc gctggttgct acttccgcga
    28381 aattgtagtt ttaggtcacg tttgcttaat tcaaagcgtc cgtcaacaag gatatcgatt
    28441 tgcgaaagga gttctaattt atcttctgaa tcttgtagca attcatcaaa ggtatagcct
    28501 gtccatgacc agatatcttt tgtcgtaccg aactcttttc ggatacgtcg tacgacttgc
    28561 agacaaacat ctgtattcaa aaaaggttcg ccaccaagta aagttaatcc ttgcacgtaa
    28621 tcgttgcgca agtcagcgat gatttgttct tctaattcag gtgtgaatgg tttgccatat
    28681 cggaaatttt gtgcagcttt gttgaagcag ccttcgcagg caaataagca gccactaacg
    28741 taaagactgt tgcggacacc ttcaccatcg acaaaattaa acggtttgta gtcagcaata
    28801 tagttttggc tgtagtcttt tgctaaccat tcttttggtt ttggatttct cataaaaaaa
    28861 cttctttcat ttaaggttca aagatatagc tattgaagat aaaatctact cgaccacata
    28921 aataagaaca aactgaccta aatccattgt tcaagaaagc ttgttcttat tttgatagag
    28981 ggcgttcaaa gatacaaagc catcgaaaga gggtggtgaa aaaagacttg tcatagcctc
    29041 ttattctgaa taaacggtgt tcaaaagctt gcatctgcag taataagccc aactaaacga
    29101 aaaatatgag gaactatttt tgtttagcta ttcttattac ttgatgcagg acagcttttt
    29161 cacaacctca tttcatgtgt ttcacacgtg aggaaatttc tttatggcgg ccatgtacca
    29221 tcggacgagc ttgcggattt cctaaatagc cacaagtacg tttgactaca tcacatgttt
    29281 ttggatcatg atttccacat tctggacatt cgaaccctcg ttctgtcggt ttgaaatcac
    29341 cttcgaaacc acattgatag caatgatcga ttggcgtatt cgtaccaaga tacccgattt
    29401 tgtcatatgc aaaatcccat acagcttcta aagctttcgg attttgttgg agcatagggt
    29461 attcacagta gtggatgaat ccacctgaac aatattttgg ataatccttc tcaaattcca
    29521 atttttcaaa tggcgttggg tttttacgca catcatagtg gaaactgttt gtataatatt
    29581 ctttatctgt gatattttcg atgatgccga atttttctgt atccaaacgg cagaaacggt
    29641 cagttagact ttcacttgga gttgaataga cactgaaatg ataaccatat tcatcccccc
    29701 atgcatctgc attagctttt aattctttga cgatatcaag tgtgaaagct tttgcttctg
    29761 ggtttgtttc ccattcgcca ccgaagaaag ctgaagcaac ttcatacaat ccaatgtagc
    29821 ctaacgatac agtagctcga cggtttttga ataattcatt gacagaatcg gtacgtgaaa
    29881 gacgtttccc gaaagcacca tacatatata aaattggcgc atttgctgga atggcttctt
    29941 tacaacgttc tacacgatag agaagcgcat ctttcatgat ttctaagcgg tcagacagta
    30001 actgccagaa tttattctga tctccttttg attcgatcgc gattcttggc aaattcaatg
    30061 taacaacacc taaattcatt cgtccgacat ttacttcttg accattttcg tctttccacc
    30121 cttgcaagaa tgaacgacag cccataggta ctttgaaact tccagttaat tcaacgattt
    30181 tatcgtaatt cagaatatca ggatacattc ttttcgttgc acattccaat gctagttgtt
    30241 tgatgtcgta attaggatct tcagggttta agttcacacc ttttttcaaa gtgaagatca
    30301 gtttagggaa gatagctgtc cgcttttcac tccctaatcc attgattcga atctgtaaaa
    30361 ttgctttttg aatttctctt tcaaaccagt tttctcctag tccaaatcct aatgaagtga
    30421 aaggagtttg tccatttgaa gtaaataagg tattgatttc atattccaaa ctttgcatag
    30481 catcgaagat atcttttttg gtttttgctt ttgcatattc tttttggcgt tctggcgaat
    30541 cgatccattc ttccgcgtct tttagatgtt ttttatagtt caattctgca aaaggagcaa
    30601 gcaattcatc tgttcggtcg gcagaacagc caccatattg acttgacgct acattggcaa
    30661 taatctgtga gatttgtgct gtggcggtct ggatagattt tggcgattcg acttcggcat
    30721 tgccgatttt gaacccattg ttcagcatgc ctttgaaatc aatcaagcag caattcgtca
    30781 tcggtgtata aggatgataa tccaaatcat ggtaatggat atcgcctttt tgatgcgcat
    30841 ttgctatatg tggtggcaac attttcagtc cgattgattt tccaacgatc ccggctgtca
    30901 gatctcgctg tgtattgaaa acattactgt ctttatttgc gttttcgttc acgacagttt
    30961 ggtccttgtt gattagcttt tcaatcgaaa agttgatatc tgttgcttta ctgcgagcaa
    31021 aatctcgttt ggtacgataa ttgatgtact cttctgctaa ttcatattcg tttttttcta
    31081 gtaatatatg ttccacgatg ttttgaatct catagatttt gacattttca gcaaaccggc
    31141 ttttgatttc ttggacaacc aagtctgcaa tcatttgaat tttttcataa gtaagtggtg
    31201 ataatgagcc gtgaattttc ttttctgctt tgatcagtgc ttcattgatc ttttgttcat
    31261 cgaaatcaac atggcggcca tcgcgtttga tgactttcat ctgggatgtc tctggctgct
    31321 gagtaacgac agttttttct tcataattta ctttcattat cttcaactcc tgctaaattg
    31381 ttcttgtcta tcataaaaca aaatcgggga aggagcaact aaatatcggc tatatatagt
    31441 attcaatgtg ctgtataatt ggatttctat actatatgta gtgtatattt tattgtgaaa
    31501 aaaggtgaaa acttgtagct ggtggatttg ttttttgcat atttaatttt gctgaataaa
    31561 ataattgaat aaaatttttt attcagtcca agtaaaagtg acaaaaacat aagagcaaat
    31621 tattcgtaga tatgctacta tgcaaataaa tagaaatgaa aaatgaaaga ggaggagaag
    31681 aattggaaaa gatgatcgaa gtgaaacatc taagtaaaag ctacggcagc aaactttctc
    31741 ccgtcaaagc cttagataat atctcattca cagtagaaga aggcgagttt gttgggatta
    31801 tgggcccaag tggtgcagga aagacaacgc tgatgaatat cttgtccacg atcaatttgc
    31861 ctacgatggg cagtgtctcg atccaaggaa aagaaatcac aaaaatgaaa aacagtgagc
    31921 tcagtgactt tcgtcgaaaa aaactaggat tcatttttca agagttcaat ttgatcgata
    31981 ccctaaatgc aaaagacaat atcctattgc ccctagcagt tgaacggatg actaaagagg
    32041 aaatggaaaa gagagtaaga catgtagctc aattgttgaa tatagaagaa ctgctcaaac
    32101 ggtatcccga tgaactttct gtcggacaga gacaacgaat tgcagctgct agagcacttg
    32161 ttgttcagcc acaagtgatt tttgcagatg aaccaactgg ctctttggac tcgaagtcgg
    32221 ctacagaatt gttgaactac ttgaagacca tgaatcaaaa agaaaaagca acgatactgt
    32281 tagtcaccca tgatccatat acagcaagct actgcgatag aattttattt attaaagatg
    32341 gtgtgatttt ttctgaagtt gtcagaagag ggacaagaag agagttcttt gagaaagtaa
    32401 tagacatgca ggcgacgatt ggcggaggag gaaaaatgaa tgctgtataa actagccgtt
    32461 aaaagttttt ttcggcagtc acgaggctat cttgtttatt ttttcagttt gactttatcc
    32521 gtaatgatct attattcctt cagcgcaatg acctacgatc aatctttagt cagaagagca
    32581 agtcaagata cttcgataga tggtggattg agttttggtg gatttatcat tactgttgtt
    32641 ttgctttttt tcgtattcag tgccaaccga tttttcttga atagaaggca aaaggaaata
    32701 ggcatctatc agctttttgg tatgaataaa ctgcagatat caggtatata tgtgttagaa
    32761 atcatgataa ttggattgtt cgcctgtatt tctgggatat tactcggtat tattttttcc
    32821 aaactctttt caatgatttt ggttcgaatg atggatatgg atcttactag tccatttttt
    32881 atctccattc catcagtagt agatacttta tttgcttttt tcatcatcct tttagcagta
    32941 tctgtatatt caatatggaa aatatggcgc tatccaatga ttcgaagttt tggagaaaaa
    33001 gaacaagcag atagttcaac aatgcgcttc aggacaaggc accgattact cgcagtcatc
    33061 gggttactac ttttaacgag cggatatttt ggagctagtc attttaggga aatcacttca
    33121 tggatgatca gttatcatta tcttgataca ttagttttct tttttccttt tttaattttt
    33181 ttcgtttgtg tggtcggtac ttatttgttt ttctgttata catttcggtt atttttgatc
    33241 atattgagta aatggaagat ttattactat ggtatcaatt ttttgataat gggtaacacg
    33301 caagttcatt tgctcaaaag ttggcgtaca aattctctga ttacagtcat tctcggaatt
    33361 tctttaatga caattggcgg cacggctagt atctctacta ttttatccta ccaggagaaa
    33421 gtttcctccc cgatggacta tcagtttgat gttgagacag agaaaaaaat caagccgatt
    33481 ctagcagagg aagggcagaa gataactaaa gaaatcacct tgcattataa agtcattggg
    33541 agtatctata gtcaaaatct cgaagaacct atgtttcaaa ctgctaatct gatcacggaa
    33601 acacaatatc aagccttcag aaaagttaat ccagatttgc cgaacatttc tttgaaatca
    33661 cctggtcata ctgttttgct tgatcaaatg caaactcttt ttcggaatat ttcattatat
    33721 ggatctagta ttggactgcc agataatcaa gaattgaaga ttcaacaaat gcttccaagt
    33781 gtattaggtg acgaaacaat ggtatatcta agtcctgtac tgattgtaga tgataccgtt
    33841 tttgaaaaag caaaaggtgt ggattatcaa gtagtcaatg tagatgtatc aggtggaaat
    33901 aatgaaaaaa tagatgaacg actgagcaat gacctgtcga taaagtggct caatgctgtt
    33961 tattattctt atgatatcgt aaatggaagt ctacaaggag aaattagcac aaagaaactt
    34021 ccggatgacc aaatgaagga agcacaattt agccaagaaa gctctcgttt gaatttctct
    34081 tccagctatt cgaaattgcg tgcagttaat cggcgaatcg gtatcaatat ttttgtcaca
    34141 ctatttgtcg ggatgatcgt gattattgct actggtagta ttttgacagt ccggcaatta
    34201 gcagaagcag aggaagaaaa agaaaattat cacctattga ctaagctggg gattccacaa
    34261 agaaggatca gacggatgat cttcgagcaa aacgcactta ccttctttcc acccatgata
    34321 ttagggatca ctcacgcagt atttgcaatc aatgtcttca ctcagtatat aaaaactgcc
    34381 gattactggt tggcttatct tgtgagtggc ttattgatcc ttatctatct tttgctttat
    34441 gtcattacga gtcagttata ttgtcggatt atcgaggaat gatatacgga ataagacaag
    34501 ataataaata gaactaaatt ttgaatacca tttatttgga ataagaggtt gtgacacgat
    34561 ttttgtcaca acctctttat tttttatatt
//
LOCUS       DAZABD010000028        33127 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000028 DAZABD010000000
VERSION     DAZABD010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33127
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.028"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(94..951)
                     /locus_tag="ACHALG_001378"
     CDS             complement(94..951)
                     /locus_tag="ACHALG_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFN2586798.1"
                     /translation="MKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTISSSG
                     AENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELEEALE
                     AAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQQAKE
                     FNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAASFQRI
                     KDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGVLVAA
                     AYGIIRKKK"
     gene            complement(1059..2362)
                     /locus_tag="ACHALG_001379"
                     /pseudo
     CDS             complement(1059..2362)
                     /locus_tag="ACHALG_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(2359..2868)
                     /locus_tag="ACHALG_001380"
     CDS             complement(2359..2868)
                     /locus_tag="ACHALG_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2586799.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(3043..5178)
                     /locus_tag="ACHALG_001381"
     CDS             complement(3043..5178)
                     /locus_tag="ACHALG_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFN2586800.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(5261..6916)
                     /locus_tag="ACHALG_001382"
     CDS             complement(5261..6916)
                     /locus_tag="ACHALG_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFN2586801.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(6918..7232)
                     /locus_tag="ACHALG_001383"
     CDS             complement(6918..7232)
                     /locus_tag="ACHALG_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFN2586802.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            7402..7662
                     /locus_tag="ACHALG_001384"
     CDS             7402..7662
                     /locus_tag="ACHALG_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HFN2586803.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            7676..7990
                     /locus_tag="ACHALG_001385"
     CDS             7676..7990
                     /locus_tag="ACHALG_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HFN2586804.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(8720..9274)
                     /locus_tag="ACHALG_001386"
     CDS             complement(8720..9274)
                     /locus_tag="ACHALG_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFN2586805.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(9400..11328)
                     /locus_tag="ACHALG_001387"
     CDS             complement(9400..11328)
                     /locus_tag="ACHALG_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFN2586806.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            complement(11634..12350)
                     /locus_tag="ACHALG_001388"
     CDS             complement(11634..12350)
                     /locus_tag="ACHALG_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFN2586807.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(12351..14174)
                     /gene="glpO"
                     /locus_tag="ACHALG_001389"
     CDS             complement(12351..14174)
                     /gene="glpO"
                     /locus_tag="ACHALG_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HFN2586808.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLIPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(14188..15684)
                     /gene="glpK"
                     /locus_tag="ACHALG_001390"
     CDS             complement(14188..15684)
                     /gene="glpK"
                     /locus_tag="ACHALG_001390"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFN2586809.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(15820..17280)
                     /locus_tag="ACHALG_001391"
     CDS             complement(15820..17280)
                     /locus_tag="ACHALG_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN2586810.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(17270..18565)
                     /locus_tag="ACHALG_001392"
     CDS             complement(17270..18565)
                     /locus_tag="ACHALG_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFN2586811.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVKEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(18897..19574)
                     /locus_tag="ACHALG_001393"
     CDS             complement(18897..19574)
                     /locus_tag="ACHALG_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HFN2586812.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(19604..20695)
                     /locus_tag="ACHALG_001394"
     CDS             complement(19604..20695)
                     /locus_tag="ACHALG_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFN2586813.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(20796..21230)
                     /locus_tag="ACHALG_001395"
     CDS             complement(20796..21230)
                     /locus_tag="ACHALG_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFN2586814.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(21814..23271)
                     /locus_tag="ACHALG_001396"
     CDS             complement(21814..23271)
                     /locus_tag="ACHALG_001396"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFN2586815.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENL
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIEDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(23268..25163)
                     /locus_tag="ACHALG_001397"
     CDS             complement(23268..25163)
                     /locus_tag="ACHALG_001397"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFN2586816.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPITLLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSPVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(25221..26996)
                     /locus_tag="ACHALG_001398"
     CDS             complement(25221..26996)
                     /locus_tag="ACHALG_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFN2586817.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYLVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESSEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(27046..28542)
                     /locus_tag="ACHALG_001399"
     CDS             complement(27046..28542)
                     /locus_tag="ACHALG_001399"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFN2586818.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     EIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRIWKEEDCAPVMLDGDEQYLQANTVDYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(28736..30358)
                     /locus_tag="ACHALG_001400"
     CDS             complement(28736..30358)
                     /locus_tag="ACHALG_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFN2586819.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESINAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTVPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(30808..32121)
                     /locus_tag="ACHALG_001401"
     CDS             complement(30808..32121)
                     /locus_tag="ACHALG_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFN2586820.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCILMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLLGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFMGFMWILNVK"
     gene            complement(32366..32590)
                     /locus_tag="ACHALG_001402"
     CDS             complement(32366..32590)
                     /locus_tag="ACHALG_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2586821.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(32610..33032)
                     /locus_tag="ACHALG_001403"
     CDS             complement(32610..33032)
                     /locus_tag="ACHALG_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFN2586822.1"
                     /translation="MESVYRQERKSNRKKGQTMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGVLTFNFSDPEGNYFVIGKAESSD"
BASE COUNT         9462 a         7106 c         5512 g        11047 t
ORIGIN      
        1 aataactaaa gagagatggg acatgtagtg gcactcaaga attagatttt tcagtctaac
       61 ttcgaggtac tctaccttcc catcctctct tttttatttc tttttgcgga tgattccgta
      121 agctgctgcg actaacacac ccgcccctgc taaggaataa ctgacagaag ctgttttttc
      181 atttgttttt ggcaaagtct gacttgttga tttggatggc tctgtcgttt gctgaactgt
      241 ttctgtcgtg cttccagagg tcactgattt ttctcctgcc gaatgcaaaa tatcttttat
      301 tcgttggaaa ctagctgcta agtcagcttt ttttgcttca aaatcattag caactgtttg
      361 tgcttcgctg atctttccgt ggattgcttg acttgccgct gtgatatccg cttgagtttg
      421 acttagcttt ccagaagctg tttgagcaac ctgatttaat tgctgattga attcttttgc
      481 ttgttggatc ttcccgtcaa gatctgtttg gacagcttcc gcttttgctt tcgcttcggc
      541 aagctttttc tcaagttgtg cacggtcatt tcccacttta tttttaagat cagctaaatc
      601 ttgatcgatt gctgcttggt cttctttcaa tttgtttttt gcagcttcta atgcttcttc
      661 gagttctttt cttttatttt ccaagtcttg caaatgatcg atttggtcag aagaggtaga
      721 actatttatt gatgtatctt ctgaagatga aatcgtttct cctgaattat ctgtcggtac
      781 attggaagtc atttctgttt ccgaggcacc gttttcagca ccagaagagc ttatagttgt
      841 tgaaggttct gtttgagcag gttcagaagt ttccgcaaaa gctgtctgtc ccaataagaa
      901 aatactggaa acagcaattg gccagattaa cggcttcatt ctttttttca tcacttttcc
      961 catccttact actttttaac taatttctta ttataagcaa aaatgtttgg aagaaccata
     1021 aacacaacag gctgtttgtc taaaaaacgg tgcatctttt atttttttgt acgttccggg
     1081 cttttccatg tcccgcttcc ataacctaaa atcgttttga ctgctggaat gaaagtcgtc
     1141 acgatcgtta tcgcgttcac tatccagaac aaaagcatat atagtggtgc gaaaagaagg
     1201 tatttcaatt ttcttcgtcg atcatctaca atcagcgaag ctagcaactg gaaaaatcct
     1261 gcgaccattt caaagcagat gaacacaaaa ctgaatgtaa aagtaatata aatctgttcg
     1321 tagtttcctt gatatacata atgcccgatt aatccagcaa aaagaactac ggaaatacaa
     1381 aagaatattg accagacaat actcaatgtt tgatcaatga agatgatcgt tcttccaata
     1441 tgtttaaaag gatggagcat cacttttttg aaattcgtca gccagacttc tgttccacct
     1501 tttgcccaac gttttctttg tcgatatagc atcttcaacg tcacaggtac ttccatgaaa
     1561 aagataatct caggagcaaa aacagataac cagtcattta actgatgatc ccaagcaata
     1621 ctgatgtctt ctgtcgctcg atcttgtctg aatccgccca catcgatcaa ggcttctttt
     1681 cgatacatcg tatttgctcc actgtacgca taaagaccac cgaaaacagc tgactgtgtt
     1741 cgtttaatga tgcccacgat acttgagaat tcaaccgttt gagattttgc aatcaatttg
     1801 gtacgatttt ggacatccat atttgcagtg accgcagcaa tatgtcttgc accagggcga
     1861 atgaaataat tcacatacct aatcagtgca tctggctccg gtaccgtgtc tgcatcattg
     1921 cttaaaatca atttcccttt tgcaaaggca agtccgatat taaacgcgtg ggcttttcct
     1981 ttattttttt cgatccgaac cacacgaaga ttagcgtatt ttttcatcag tctagctaat
     2041 atttctggtg tttggtccgt tgaaccatcg tctgtcacta atacctcata gttgtgataa
     2101 ttcaatttcg tcatcaaata ctcgattgta tcttcgatca caatttcttc attatgggct
     2161 ggcaccatga tcgtgatgaa cggttcgaca ctcaagggaa catctaccca ttctttttgc
     2221 ttatgtttgt ataaaaacac ataacaccag acaccaataa accatacaaa tccgcctaaa
     2281 atcggataag aaacgagaaa aaatacataa tagacaaaca agtatctaag aataaattac
     2341 ctgtatgggt gattgagatc atttcagttc caccccattt tctttataca aatctgcaat
     2401 gaatgtcgta tctagatttt tctcttcgga gacagaataa aaacggaccg tttctctctc
     2461 ttcttttgtt ccaaaacgtt ccgtatacac ttcttcaagt aattcttgtc tttttttcaa
     2521 tttttcatct tcatattcaa aagatttatt tacttttttg tatagatttc gattgttcca
     2581 cctagtcaat acaaatgagg taatcaggaa actgaaaaaa atcaatacaa aaaagccgat
     2641 taagaaatac agcattttct tttctgataa aaaagcgtac aaggaaaaat ccttggctct
     2701 ttctggtctc gttaatggga aaaagaccca tataaaagga agaacaacga tcatccatcc
     2761 taaaagagcc actaacgttt gccaaatctt taaccagtaa ttaccttttg aaaaatactt
     2821 atctttcacc aaaattgatg caacttcttc tcttgttact tttttcatat cctcacctca
     2881 cgtttttttg ttacgacgta ggagtttacc atacatttca tttcttttca cataaagtgt
     2941 tcctaaattt ttcctaagaa aaagaacaaa acatttctca tcagtaaatg aaaaatattt
     3001 tgttcttttt atcagttgtt ctttttattt tttatctctt aattatgttg ctctttccaa
     3061 tacgacaatg cttttcattc ctctagatat cgccgtgtac atttgtctgc gtcttctttg
     3121 ctcatctcca tcgttttcaa atggatataa gattacatga tcaaattcca gtccttttgc
     3181 tagactcaaa ggaatgattt ggcagttttc taatccatat gaattttttt taatcgattc
     3241 ttgtagcgtt tgggtttcct cttctgtttg ggtgataact gccgcttttt ctccttttag
     3301 agaaaccaaa atctcagcta gtattttcaa caattcttca gaggatcgaa aagagcggta
     3361 ttcgggttct tttccttgcg gtcggatcga tacgatgtcg atcttctctt gatccacagc
     3421 ataggttttg aaaagctctg tgattgctcc gctagaacgg taactatttc taagatcata
     3481 ccgatggatc ggcagatggt agtcgtcgaa acaacgcata atatcagcaa aagtagtgga
     3541 agaattaaag attgcctgat tctcgtcacc tactaaagta aagtgagttt ttggaaataa
     3601 ttcgatcaat agacctaatt gtgccagggt ataatcttgt acttcatcaa tcaatagata
     3661 gcgaaatgct ggtacattta ccttttcaac aagtaaatga cgaatagcta atataatgac
     3721 tgcttcatca acagtacgtg tgggttgcca agcataagct gagccatatc gtttttcata
     3781 aattctttca aacaactgat gctcatccac ccatgccatt tcttcgattt gtctggttat
     3841 ttttcgatat tttttttgta aaagtttcct tgcataagct acaatacttt gttccgaatc
     3901 atcagaaatc aatttaccga ataattccaa ctgcctatct tctgtcaacg agagtacctg
     3961 attctgcaca gcaggacttt ttgcttgttt caaaagatga ttcttccatt cgctcatcag
     4021 tgcctttttc gttgcttgaa gcctatcgat caatttagga tgagcaggaa ctttctcata
     4081 tattttttcg atcgtttttt tagaaaggac tgtttttcca tttttagtaa gtccacggat
     4141 aaaattcgga tgatctaaaa acaaaggatc tgattgtttt agattatcga taaacttttt
     4201 gctacgcaaa cgttctgtct gctccgatac attttctccc gtgatacgtt tgaaataagc
     4261 ctcttcacct tctatttcct ctgctgaaag ctgtgaaacc agctgtatga tggttagatt
     4321 cagtggattt ctttcaccta atgatggtag gacattcgaa atatagtcga taaaccgatg
     4381 attaggtgat agaatcagca agtcatcgga tgtcatcgta tctcgatata aatagagcag
     4441 atacgcaata cgctgcatga tagcagaggt tttaccactt cctgctacgc catttaccaa
     4501 gatattttga cttttcacat cccgaatgat tgtgttttgt tccgattgga tcgtagcagt
     4561 gatagccttc atttcattgg tctcatcttg gcctaagact tctaataata cgtcatcttg
     4621 aatagctaca gacgtatcaa aaaacttgac caaacgatct ctttccaaaa gaaattgtcg
     4681 tctttcttta ataaccgtat cgattttatt ttgacgaacc gtataagaag atggaccaat
     4741 catgttattg taaaaaagtt cggcaattgg tgaacgccaa tcataaacaa gattttcatc
     4801 tgcatcatct gtgaaatttg ctgtaccgat ataaaaggat tcttttggct cattatccag
     4861 aaagtctaca acgacttttc caaagtaggg acttttcaat aaacgttcga tctttgctag
     4921 ctgattttct gcgtttttct gtttgatatt catctgatcg atttctctat ttttcatttc
     4981 aatcatcgaa aaagtatcca ggttatctaa gtaactgtca aaattcaatc gaagctcgcc
     5041 agccatttct ttcaacgact ggtttccttc tatttttgcc gtatccatcg attcccgcag
     5101 ttcgtttttt ttatcaatta gcttttgata cacgttcttc agatatgctt cttctaactg
     5161 tttttgtgtg ttgaccatac acattctcct ctttttttaa agtggtggaa ccgattagtt
     5221 tacctctttt aagagtcgaa tgcaagcttt ttaacgagta tcatttgtta ggatatatca
     5281 gttcttcagg tcggacagtt tgatataaag aaaatgcacc atcaaggttt tttacgtggt
     5341 agccagactg cttcaagatt ctttccgcta gatagctacg taatccgcta tgacagctga
     5401 cgatatattc ttgttggctg tctaattcat ttaatcggtc ccgaagttca ttcaaaggga
     5461 tatgtttggc ttccttgaat ttgccctttt ccaattcttc tgctgttcgc acatctaata
     5521 aaatttttcc ttttgctagt tctgtcggca attcatgcca ttgtacggtt tcactaagcc
     5581 cttctgcaat gttcattgct gcatatccta gcatgtttac tggatctttg gctgagccaa
     5641 atggcggtgc atacgtcaat tctaattccg gtaaatcgaa gatcgtcaaa tgccctttga
     5701 ttgctgttgc taaaatatcg atccgtttat ccacaccttt tgccccaaca ccttgtgcac
     5761 cataaatctc gccagtctct ggatggaaga tcaacttcag taaaatatct gttgcttctg
     5821 gataatagct tgcatgatct tttcctgata catgaatgac ttcaaaagac agttcgtttt
     5881 ctttggctgt tctttcactt aaaccggtgg atgcggcagt cattccaaac gttcgtataa
     5941 ttgctgttcc aataccgcct tgatttcttc gagaaattcc ggaaatcgtg tccgcaactt
     6001 gtctaccttg acgattggct ggtgaagcaa gagaaataag tgcatctttt ccagtgattt
     6061 gctgttttac aacgatagca tcccccaccg cataaatatc aggaatattc gtttgataac
     6121 gttcgtctac taggataccc ccgcgtatgc ctaattcgat tccagcggtc actgctagct
     6181 ggttatcagg acgaacacca atggataaaa taactaaatc agcagggatg gtacgcccat
     6241 catccagtcg caatcgtttc ccgtggtctt caaatccagc gacagcatga gaagtaatca
     6301 cttctactcc ttgatgagac aattcttttt ctataaaagc tgccatttct tcatccagtg
     6361 ttggaagaat atgtggtgct tgttcgatca ccatgacttc taaacctctt ctttttaggt
     6421 tttctgccat ttccaatccg atgaatcctg caccaacgat cacggcacgc tttggctgtt
     6481 tttctaatgc atgcatcact ttatcgatat ctggtacatt tctaatagaa aaaacattgt
     6541 cggcttctgt tatccctgga atcgatggaa caactggttt tgcaccagga gataaaacca
     6601 atgcatcgta ctgttcaatg tgtttttgac cctcatgtac gacttctacc gttttgtttt
     6661 cgggaaagat ggctgtcact tcatgatttg gacgcacatc taaacaaaaa cgagcggata
     6721 atgattctgg cgtttggaca agaagatttt cccgttcact gatttctccc gaaagataat
     6781 aaggcaagcc gcaatttgca aaagaaacat acggtccttt ttcaaaaaca acgatttctg
     6841 catcttccat taatcggcga agccgtgtag cagcagacat tccacctgct acacctccga
     6901 caataacgat tttcatctta tttccctcct actgttcgat caaaccattg attcatgcca
     6961 ccacgtacat tcactacatc ataaccagat ttctttaatt ctttagctgc ttgtttactc
     7021 ctcatccctg attgacaaat cacatagacg gtcttttctt tgtctccatc atatctgttt
     7081 atactttgta atggaacatt tttagctcct tttatatgcc cgccccggta ttctgcaggt
     7141 gttctaacat caagtaacgc tggattttcc aaaagctttt cctttaattc tgggactgtt
     7201 atcgatttta ttgattgaaa tagcaaattc attttctgca cctcatttac atttttataa
     7261 atacctcatg gggtatatta aacaggagct ttattaatat gtcaaccaga atgactcttc
     7321 atttttttca ctacttttta tatttgcata aaataccgat aaggggtatt atctaattat
     7381 aaaaatagag gagaatgtgt tatggagatt gataataaaa atatcctgaa ccgtttgaaa
     7441 cgtacagagg gacaaattcg tggtatccaa aaaatgatcg aagacgaaaa agaatgtatg
     7501 gatgtcatca ctcaattaag cgctgttcgt tctagtattg atcgcgtaat gggcatgatc
     7561 gttgcagata atctgaaaaa ttgctttgag aaacctgata gtaatccaga agaacaagcg
     7621 gctaaactag aacaagcgat caagatgatt attaaaaagt aaaggaatga tttcaatgaa
     7681 agtcgttttc catattgatg aattagaaaa atggtcagaa acagggaaaa acgtaaaaaa
     7741 cctgataaaa gcttctccag aaacgacgat tgtcgtatct gtaaatggca tcgcgattac
     7801 cggttatcta gactctgcca atgcagaatt cttagatttg caaggagtcg tttttcacgc
     7861 ttgcgccaat gctatgcgtg ctaatcatat ttcagaaagt tctttacctg aacaagtaat
     7921 cgttgtacca gcaggtgtcc ttgatttagt tgaactgcaa tcccaaggtt atgcttatat
     7981 caagccttag tcagtagagg ttgcgacaga agtgatcagc ttcaagaaat ttcggcttat
     8041 ttttcgaaga agctacttct ggaaaaccgt gaatcaaagg ttgtgaaaac gctgtcctgc
     8101 atcaagtaat aagaacagcc aaacaaaaat agcttctcat atttttcgtt tggttgggct
     8161 tattaccgca gatgcaagct tttgaacacc gtttaaggaa gaggttgtga aatcagcggt
     8221 ttgacctaag tattagagaa aaaaacggga aaatagctcc tcatattttg ccgttttttg
     8281 agctaatacc gaaggtcagc tgattgaaca ccgtttaagg aagagattgt gaaaaagtcg
     8341 cttagtttcg agtattaaga aaaattttgt gaaaatggat ttctacattt ttgcataaat
     8401 ttggcttaat accgagaaac tggcttttga ataccgttta aggaagaggt tgtaaaatca
     8461 gcggtttgac ctgagtatta gagaaacaaa cggaaaaata gctgctcata ttttgccgtt
     8521 ttttgagcta ataccgaagg tcagctgatt gaacaccgtt taccaagagg ttgtgagcct
     8581 accatttaac tccgaatcac aagaaataat ttcaaaaaca acttctcctg tttttggttt
     8641 ggttggctta ttatcgagaa gtagctactg ggagtctatt tatcaaaaaa aggaatgcga
     8701 cataggtcac accccttttt tattttcttt gaaacatctt tcggtactcg acagcataca
     8761 agacaaaaga ttgtaaaaga ccgtaagtga tgacggcaaa aatgactctc acccacagcg
     8821 ttggtaattc tgggaaaagc agcaaaagga tcatgccaac atagatgaca gcagtcgcta
     8881 ctttgccata ccacttcgct ccacttaatt tgatattttt agatagcccg attaatccag
     8941 ctacaaacat aaagccttct tttatcaaga atagaccaaa caacagccaa agccacggtc
     9001 gataccatgc catagacaaa atcagaaaaa gttgtgtcaa tttatctgca aacggatcaa
     9061 gcaccttccc taagtctgtt acctgattat atctccgagc aatgaccccg tctagataat
     9121 cagacaaacc tgagacaaca atgaccgctc ctgctatata aaatgactgt ctcagcacaa
     9181 aataaagata aatcggcagc aaaaaaatac ggaatatcga taaaagattc ggaatcgttt
     9241 gtatttcttt acgccagtct gtgcttaatt tcattttatc gccctacctc ctcagcactc
     9301 ttttcttttt attttagctt acacggggaa taaaaccaat gatttcactg tttttatttt
     9361 tctttacagc cactgatatt atgagctaat ttgagataat cacttgctgt tttcatcgtt
     9421 tactcttctt tcttcctgtc gtttgcgcat ttcttttact agtactttat gagctttttt
     9481 tgatcctcgc tctaggtcac gctgccgttt acgtgtttta taatcgataa acagactgtc
     9541 taaatcgtaa cctgtcgggt acagatcttc tgctgaacgc accagacgaa cacgctgacg
     9601 aggcacgtgg attatttttt tgtctaaata tacatcaatc gtatcttcac ctatatcttc
     9661 ataaactaac gcttcttttt ggtattcatt gacaaatatc cgatcacctt ttgcaaagag
     9721 ctggcgttct tttttattct ccttcctatt tgtaactgct gtcttaggaa gaaattcttt
     9781 tttctgtgtc cgataggctt tttccgccaa gtaacgctct gcttgatgga tcaatttcat
     9841 cgacatatcc attttatgag caatccacaa agcttggctt tctcctgttt ctccaacccg
     9901 caactgatat ttaggacgaa gtgcttcccg atcgaaagcc atagcagccg gcacaaaatc
     9961 ttcatgatct ccggcgaatt tcttgatttc tccataatgg gtcgtggcaa tgatcaatgc
    10021 cccttgctca tacatagatt ccatgattgc gatcgctagt gcagcccctt catttggctc
    10081 tgttccactg ccaatctcat ctaaaagtac caacgtattc ctttttatct ttttcaaaat
    10141 agcagcgatg ttttgcatat gaccagaaaa tgtactcaac gcattttcta aattttggtg
    10201 gtcaccgaca tcgacgaaaa tctcatcaaa gacagcaagc tctgtccctt cttttgccgg
    10261 aacttgcata ccgaacatcg ccatcaaagt caataaaccg actgtcttca acacgactgt
    10321 tttgcctccg gcatttgctc cggtgataat caagcctcga tagtctttac cgatttcaac
    10381 atccaatggt accgcatgct caggtaaaaa tggatgtctg ccttgcttga taatgatatg
    10441 ttccgattta ttgattctag gtgtgcttcc attgatttct cggctaaatt ttgcccgagc
    10501 aaagatgata tctaacaccg tgatcgtttc gatcaataaa tcgatcactt cttcgttttc
    10561 tgcgatagca ccagtcaatg ccgccaggac ttgatattct tcactgattt cttcagcttt
    10621 taacaactga tagtgttcat tcaatttaga aatcacagtt ggttcaataa aaaccgtcgt
    10681 tcttttattt gattcatcga taatcgttcc atctatttta tttttatagc tggctttgat
    10741 aggaatcgta tagtattcgc ctttttgaac aatcattgct tcttggatca tttctttgtt
    10801 tttcggatga cgcaagaatt tcagtaattt tgactggatt tctttttcga ttagttgtaa
    10861 ttgtttgcgt actttacgca aattacgtga cgcgtcgtca ctgactttat tattttttat
    10921 tttctggtca atcaactctt ctacatttat caaatcagga agatgtttgc tataagcaaa
    10981 caacaagggt gtctggtatt gattcttatc aaaaaacttt gtgatcatcc gactgcttct
    11041 aagaaaatcc gcatattcga tcaaatcggt tggctgcaga atcaaccctt ttttgacttg
    11101 ttctgtcagt gcatcgatcc tcggcaaccc cataaatgga acatgctgac tgctttctaa
    11161 gatcaagcgt gcttctcttg tttcttcttg acgatccaag acagtttgta ggtttgtcga
    11221 gaccgtcata tcgctgattc gttttttgct atagtttcca attgctcttg cttggatttc
    11281 ctttttgatc cgatcaaatt gtaattgact aatcatttcg ttgtccatat tctctccttt
    11341 tcttgaagag atacctgtcc caaaataaat aagggcctag agataaataa aaggatagaa
    11401 cacagcttaa aaaagtcatg tcctatcctt attagaatac aaagatattg ttttcctgac
    11461 aggtaccgtt tcataagcac accaaataaa ctttgtttat tggctattcg acttactgat
    11521 tagataccag ctacactcac aaaacatacc ccattcttct aggtcttatt tattttccac
    11581 ttaactatag caagacacat tttggttgtc aaactttagt atgttttata gatttacacc
    11641 attaatgaat agacacctac cgcgataatt gctccgatca ttggacctgc gattggtacc
    11701 catgaatatg cccaatctga agttccttta ttagctattg gcaataccgc atgtgctaat
    11761 cgaggaccaa agtcccgagc agggttgatt gcatatcctg ttggccctcc taatgaaaga
    11821 ccgatggcta agatcaatcc acctacagcg aatacgttcg ttccaggtgc aaattcgttt
    11881 tgtccaaaag ctagcaaccc aagaactaat acaaatgtac caattaattc tgtgatcaca
    11941 tttgctggat aattccgtat agcaggacct gttgcaaacg ttcctaaaat cgcgctttca
    12001 tctttcgttg cattccaatg tggcaaataa gaaagccaaa caagaatggc ccctgcaaaa
    12061 gctcctaaaa cttgcgcaat gatgaaagga acgacaagat tccaactaat tgcccctgtc
    12121 atcgccatag ctagagagac tgcaggattc aaatgagctg ggcccatgaa tccagaaata
    12181 tagacagcca ttgttacagc gagtccccaa ccaaatgcga tgacaatcca gccagaagcc
    12241 tgtgctttac ttttgtttaa attcacagcc gcacacacac catctccaag caaaactagg
    12301 atcagtgtcc caataaactc ccccatgatt tgagtcatat cactgttcat ttatttctcc
    12361 ccctttagtt cccgcagatc tgattctgca atcaatgctt ctaaacgttt agtctgttgc
    12421 gccttctcct cttctgtcca ttcaaaatat ccagcaattg catcgatcac aggttgcttg
    12481 atctcatcaa gctgatctcg ttcaaacaac agatgatttg tacgacgaat gagataatcg
    12541 cctggggcca aaaccatttc ttcttctaaa ccataacgta accgagctga ctcagcgaga
    12601 ctcaaaccag gataaggtgc catttctttt gctaattcaa agatacgtcg ggcattagtc
    12661 ccgtaaaaat cagcgatata agtagcatct tcttcctcta aaccggctgc tactccttct
    12721 tttgctaatt ctgtcactgt ttcttctaat ttagttggat cgaagtttcc acctgaaatc
    12781 tgatattttt tagagtcgat ttctttcgtc tctattccgt attcttcttt taataattgg
    12841 cgtatcaatc gcaaagctcc ttctgccatc ttacggtaat cggtgatttt cccaccagat
    12901 aaagtcagca aaccatctgg ttctctttct aaagaactac ctctggaaat cgtagaaggt
    12961 gctttttcat cacggctgtt ttccaaatgg ttcaacacgt cttctacttc agcacgagaa
    13021 actttgtttt ccttatattc acttactgta tcgacaactt tattgaagct cttgtctgaa
    13081 atcgatccat tatctccacc attgtaatca gaaccagaat taccaatcaa cagtggacga
    13141 agtcctgccc agcttgcttc gatatcattc aatgtgatat ttgcttctgg atagcgatga
    13201 ttgatcacat ccaatagata atctacatct tcttgtgtga ctttcggatc cgtaaaatct
    13261 ccttggtaat ccgtatctgt cgtaccaaag taagtcttgt tttctcttgg aatagcaaaa
    13321 accatccgcc catcttgctt tcctgtatcg aagtatgtgg gttgcggtac aggcagtttt
    13381 ttcgcatcta caactaaatg gatcccttta gttggacgca ttttaggaga gactggacgc
    13441 gtaaaattca agtttcgtac tttatctacc caaggaccac tcgtattgat cactaatttt
    13501 gctttgattt cgatcacttc atctgtcagc aggtcacgag ctttgacgcc aacgatttga
    13561 tcgccatcat ataaaaagcc aaccgctttc attttactta ctagataagc cccatcttcg
    13621 gcagcctttt tgatattatc aatcactaaa cgggcatcat tgttgcggaa atctagatag
    13681 acacccgcgc cttttagccc ttcttttttc aaaaacggtt ctctttccaa tacttcttct
    13741 ggtatcagcg tataattctc atatttggtt cctgtcacat ttgccagttt gtcataaagg
    13801 tccattgcta ctttgaccga gaacatattg aaggttgttg ctccttcgcc ctcatagatt
    13861 ggcaaaagca ttggatctgg ttttgggatg tgtggggcaa tactttgtac gactgcacgt
    13921 tcaccaactg tgtcagctac tacttccaca tcaaatgtct tcagataacg aataccgcca
    13981 tgaacaagtt tggtcgagcg agaggaagtc ccttctgcaa aatcctgcat ttcgatcaat
    14041 cctgttttga ttcctgatgc tgctgactgg actgccacac cggcaccagt gattccgcca
    14101 ccgatgatca acagatccaa ttcttcttgc tgcatttttt gaatgctatc ttgtcttgtc
    14161 ttgtttgaaa acatatggat ttcctcctta ttcaaatgct tgggtagctt ttactgcttt
    14221 tttccaaccg ttgtaaagtt tttctcggcg ctcttcctcc atttgaactt caaatagctt
    14281 gccagcttca taaaattcgc ggatctcgtc tgtatctttc caataaccga cagctaatcc
    14341 tgctaaaaat gctgccccta gcgccgttgt ttccaagttt tctgctcgtt ggatcggtac
    14401 attcaagata tctgtttgga attgcatcag atattcattg tttgctgcac cgccatctac
    14461 tttcaataca ggaatcttga ttccagtgtc atcttgcatc gtatcgatga tatctctcac
    14521 ttgataagca atcgactgta aagtagcttt aatgatatct tcttttgtcg taccacgtgt
    14581 caagccaaac atagcccctc gtgctgcctg atcccaataa ggagcaccta gcccaacgaa
    14641 tgcaggaaca acataaacct catcttcgct tgtagagcgt ttcgctgcgt cttctgaatc
    14701 gctagctttt tgcaacattt gaagtccatc tctcagccat tgaatcgaag aaccagccac
    14761 aaaaatactt ccttctaaag catagtaaac ctttccattt atgccgtaac caattgtcgt
    14821 caataaatta tttttggata attgcggttt ttcaccggta ttcatgacaa taaatgaacc
    14881 tgtaccataa gtattcttga ccatgcctgg ttcgaatgcc atttgtccga ataatgcggc
    14941 ttgctggtct cctgccatcc ctgaaattgg tgcttcgctt ccataaaaat gataaccttg
    15001 cgtataacca taaacttctg agttagaagt gacttttggt aacatcactc gaggaatatt
    15061 tagtaaatct aaaatctcct gatcccaatc tagctgatgg atattgaaca acatcgtccg
    15121 actggcattc gagtaatcag taacatgtgc ttgtccatcg gtcagtttcc atactaacca
    15181 agaatcgatc gttccaaaca tcaattcgcc tttttcagct cgttcttgcg cgccttctac
    15241 atgatcaaga atccagcgta tcttcgttgc tgagaaatag gcatcgatca ctaaccctgt
    15301 tttttcgtgg aaaaattctt gatagccttc attttttaag ttatctgcaa ttccagctga
    15361 ctgtctggat tgccaaacaa ctgcatgata gattggacgt cctgtatctt tttcccatat
    15421 tactgttgtt tcacgctggt tcgttatgcc aatcccagca atctgatttg gtttgatccc
    15481 tgattcgatg aacgctccgg caataactga ctgtacagaa ttccagattt catttgcatc
    15541 atgttccacc caactttctt gcgggaaata ttgcgtgaac tctttttgag aactgccgat
    15601 gtgtcttcct ttcttgtcaa agataattgc tcgtgagcta gttgtacctt ggtcaatcgc
    15661 catgatatac gttgattctg tcattgtgat tccctccatt atatgcaaac gctttcttta
    15721 actactctta tcatactact gttttaaaaa aagaaactgt caaaatttta ttcttttttt
    15781 ctttttttga cagtttttta ttatgaagct attttttaat caattctttt cttttttcag
    15841 cgtctctatt cttcttttca atcgaataat gtcgtaaggt gacgcaaatt ctgacagata
    15901 atattcacac tgttttttgt ttctctggca aaatgtgatc accaaatcat attctttacc
    15961 ttcttcataa cttccaatct caataccgcc gattcccctc agttctctga tcagatattg
    16021 ttgaaaagca atacgataaa tcggcagact ttctaaatcg attccaatca ctactggttt
    16081 tgcaatataa aaatcgatca tcaaaagaac attggcatac tggatcatca ggtaactaag
    16141 ctcactatct tcagctcttt tcctcggcaa ttgctgcacc gctgtttcct gtaatttcgc
    16201 cagtaactgt gttaaggaat gcccgagcat tttctgctgc tgttccaaaa gccgatcacg
    16261 ctcatagact tcgatctttc caacaaagaa aaaccattca ttgttgatct gggaaatttg
    16321 gtagcctact gccttttctt ctttgatttt caaacgacga ggacgataat gccataacat
    16381 cgtttcccga atgtaagtat ctaaaacagc ggttggtaac tttttgctcc gtgtcaaatc
    16441 atactgataa aatatttctt cgtctactat cgaaaaagaa acaaaaaagc tgtaaaacat
    16501 cattgtttcg tatacattca gttctgctgc tgttcgacta agatgcatca cgatgattgg
    16561 atcgattgct tgataaagcc ggtccgactg atagagtaat ttcttttctt tcaaagttgc
    16621 gtacgtactt ttttcattca atagtctttt tttcgtaatc ccgagccagc aagcaatctg
    16681 tgcttctgct gatgccgtaa acgttgtttc taatacacgg tccaatccta gaatgaacgg
    16741 tcttttttct ggtgtatttt gcaaaaaaag cggtcgctga tcttccggga tatattggaa
    16801 tagttcataa taaaaatatc gtatctgtag ctcttctccg tataattgac cgtttttgat
    16861 ttgtaatcca aattcctcta agagctggtt caattctttg atctttcgaa aaacagaaga
    16921 ctcactgata ttaaacgctg tcgctaatcg aacgatggat acttgctgat gttctaataa
    16981 atagacgagc atcttaaact ttaagctttc ctgcaataaa aacaatagaa ttttttccaa
    17041 acttacatct tcgtccaaca caagcagcgc ctgatactca tttcgctgta cttctacttt
    17101 tttttccatc attcggccaa tcaattcaat atcttccaaa tattgatcaa acgaggcttt
    17161 tgacagttgg acatgctctc tcaaaccatt gatagaggct gttccccctt gcagaacaag
    17221 gtttcgcaaa atcaggatct gtaactgctc ttttctttct aataatgctt caatcttcat
    17281 aggcagtatt cgccaccttt gaccacacca tatctggcac acggtattct ggctggaaaa
    17341 atagctcatg ttcttctaac tgttcaaacg tccatccttc ttttactgca attgtcagtt
    17401 ggtcaagaag gtggaacatc tcttttttgc tttggagctg tgctccaaga atcttttttt
    17461 cagaatcaaa tagaaatttg agtttaactt tgctttgatt tattgctgta agcttgtagt
    17521 ctacgtatcc tgtttcaatt ttttggggat aaaaaatgct ctcttcttct gtcaaaccta
    17581 cgcttcctaa gaaatagcca aaccactgat tgccgactgg acgataggtt ccctgatcct
    17641 ttgtctgtga gccactaatt gtcttgctga catttcgtgc tgtacggaca gcgttattta
    17701 ctagagaaac atacattttt tcatttgttg gacgaaaagt tacttggatc gcatcgccaa
    17761 tcgcataaac gtctttttga gacgtttgca agtagtcatc tacccagatc gttccatcat
    17821 cattcaatgt taattgttct tcccacatct gattgtcggg gcgagaatga ttagccagca
    17881 aaacaagatc atagttctcc tttctttttt ccgtctctac aatagcggtt tcattttcgg
    17941 tcaaagacac aacttgttct tctaaaaaaa gtgaaagccc ttgatttttc atttccttta
    18001 ctaacggttc tgtcatttca ggatctagat aacgaaaaag aattgacttt cggctttcgt
    18061 aaagatggat ttgttttttt cttgatgcca acccttcagc tatttcgatt cctgcttgtc
    18121 ctgcaccaat aattgcaatt ttctttgcat tcatgatctt ttgctgaaga gccgttacgt
    18181 tttgtatctt tttaactggg tgaataagcg ttgattctgc agaaatattt cgaaactttt
    18241 gatttgaacc agtcgctaga atcaaacgat caaaatccaa tttttttcct cctgctaaaa
    18301 cgactttttt ttctttcaat cctattactg tcttttctgt gtaaacatgg attcgatagc
    18361 ggtcagtcag ttcttctttc gtgatccaag aaagttgttt ttcactggta acctttcctt
    18421 ttaatatcaa attgaacccg ctgggaatcc atcctaactt cgattgcttt tcaatcaaat
    18481 atatttcact tgttggcatc aattggcgaa gataaatagc cgcatgaatc cctgcaaacg
    18541 acccgccgac aattacaatg ttcatctgca ttcctctttc ttataaggtg ttctactgct
    18601 aacttctcaa tcagaaaaca cagtattcat aactcgcttt caaccttatg atacatagcg
    18661 atgcagcaga atcaaccctc tccatcatac cgtgttctag gtctgcttct caataatgta
    18721 ctagtggacc taattgaaga gtatcatttc tgaagagatg tttcaaaaaa tgtactctta
    18781 taaaagaaaa aaactcgcct tttacgtacc tctgtccaaa taggaaacag aaaagaacga
    18841 aaagacgagt tttagcgtgt gtaatgtaat agttagaata gacacatcat ttatttttaa
    18901 ttctttaatg cttcaacaac tgcttcgcct aattgatgag cagattgagg attttgccct
    18961 gtaagaagcc gcccatcaat ttgaaccact tctgaaaaag ctgctccaga ctgatagttt
    19021 gctccttttt ctttcaaagc atcttctgct aagaatggta cttcattcgt tgtaccattt
    19081 gcttcctctt cttcattact gaacccagtc actgtacgtc cagctaaaat agattttcca
    19141 gactcatctg taactggcaa caatcctaca acaccgtgac aaacagctga gaccagccct
    19201 tcatttcgat agatttgtga agcgactttc ccaagatcta ttgcttctgg aaaatcccac
    19261 attgttccgt ggccaccagc ataataaacc gctgcataat catttgcatc cacatcatca
    19321 ggacgtaagg tatttcccaa ggcccgattt ctgaaatctt catcttcata ataggcccaa
    19381 tcgatttcat ccatattcaa aacactttgc ggttctaaag gtacatatcc gcctttagga
    19441 ctgacaaaat ctgcatcgat cccatttttt actaatacat cataaaaatg agtcagttca
    19501 cttaaccaca aacctgtagg acgctcgatc ttttcgtact tttctacatt tgttaatacg
    19561 atcaatactt tcattttccc atcctctttc tataaaaatt gcgttattga taacgtgctt
    19621 tcaagaaatt atataatggg cttggcaaat aaagctcttt atatgcttct tcaaaaggtt
    19681 tcgttacaaa ttcatcctca cggttttcca ataatttctc tgcccagtca ggatcgacga
    19741 tcatttgctg tccaattgct gcaagttctg catatttcaa tacctgctct acatcttcgc
    19801 gattgcggat cccaccgaca ccaactaaag ggatctttcc ttgaatcgtg ttatggacca
    19861 cttcaagaat cgatttatcg ttgtatttat cagaacgagc tattcgatca tacatattta
    19921 atgaaacatg cagataatct agttttgact cacgtaatcg ttcaagaagc cagatcgtgt
    19981 cttcaaaacg gatacctggt gtttcatatt cttctggtga gaaacgatac cctacgatga
    20041 atggtttttt agcatattgt tcaaccgctg agaaaaccgt tgttaataat tcttcaataa
    20101 aacgataacg attttccaag ctaccgcccc attgatcctc tcgtctattc gaatgcggag
    20161 agaaaaattg ttggatcaaa tacgtatttg ctccatgcaa ctcgattcca tcaaatcctg
    20221 attcaatcgc tctacgtgtg gctttgccaa atgcttggat tgtttcaacg atttcttctt
    20281 cactcattgc tcgtggtgtt tccgcatcag gacgttttgc tgcaactgca cttgcagata
    20341 caggctgttc tccagataaa gttgctcgac ttgtcattcg tccaccgtgg aaaatctgaa
    20401 taatcgcttt tgcgccttct ccttggattg ctttcgctag ctcacttaat cgaggaatca
    20461 tttcatcatg agcgatgctg agttcacctg gccagccttt tccgatatct tggacatttg
    20521 cagcaccagt aataacagca ccaagacctt tagaacgttg tttataataa gtgatctcat
    20581 cttcggtgat cactccattg aaaaagctga gttgtgtcgt cataggcgac agcatcaatc
    20641 ggttcttcaa ttgaactcct gaaggtaaag taattgattg atcaaattgt gacattgtca
    20701 ttcctcccat aattcgctct gattaaccta tcgagaatca tctatcatta aatcactcga
    20761 ttagttgtac atgcaactaa tttgcttaaa aaattttatt ttttttgtaa cttatctgtt
    20821 gcatcattca ttgtctgggc taattcttta cttgcttcat ttcccaaaac tgctttgtat
    20881 gactgatgaa gttcttccca gctaccatcg acttccttag caaattggcg tccttgttct
    20941 gtaagataaa ctaatgacaa gcgaccttcg ctaactgttt tgacgtatcc tttatattct
    21001 aatttttcaa caaaacgagc aatcgttgat ggggcggaat agagcatttg tgcaatatct
    21061 ttttgtggta ttccatcttt ttcttttaat atcaataaaa taaaaccata agttggtgcc
    21121 agacctgtcc ttctaaacgc ttcatcagct aatttattca acatgcgatc tagctttttc
    21181 acgctaaaga aaagacattc atctaacgct ttttcaattg gcttttccat ttcatcccct
    21241 ccattggcgt acatatcata gtatgatttt tattttttgt caaaaaacac ggaataatgt
    21301 ccgcctcctc tgtctagatt tgaaaaaaag aagtagccat cttcaaagaa tttcggcttg
    21361 ttttcgacaa aactccttct ggaacaccgt gaatcagagg ttgtgaaaaa gtcgtttagt
    21421 tttgagtatg aagaaaaatt ttgtgaaaat ggctttccac atttttgcat aaatttggct
    21481 taataccgaa aaactggctt ttgaacaccg tttatatcag aggttgtgaa aaagccgtcc
    21541 tgcatcaagt aataagaaca gccaaacaaa aatagttcct catattttgt cgttttttga
    21601 gctaatgccg atggtcagct gattgtacac cgtttattga gaggtcgtga gcctgccgtt
    21661 gaactccgag tcacaaggag caatttcaaa aaacagcttc tcatgtttta tgactatggt
    21721 gacttgtgat cgaggagttg gcaggagaat actgtttatt cgtaaagaag gggctgtgac
    21781 aagacttttg tcacagtccc ttatttcctt ttattatagg atttcgccat ttgattggat
    21841 gacttgtctg taccaatcaa atgatttttt ctttaaccgt ttctttgtgc cattcccttc
    21901 gtcatcttga tcaacataga tatatccata acgttttgac atttgtgaag tagaagcgct
    21961 gacgatatca atgcagcccc acgacgtata accaaataaa tctactccat cttcgatagc
    22021 ttccttcatc tgctcaatat gctgcttcaa atagtcaatg cggtaatcgt cttgaacggt
    22081 tccatcttct tctactttgt ctatagctcc gagtccgttt tcagctatga acagtggttt
    22141 ttgataccta tcatataatt gattcaaagc aattctcaaa cccttaggat caatctgcca
    22201 gccccattca cttgatggaa gatacggatt tttaattcct ccagttatta agtttccttg
    22261 gaccccttcc aaattttctg gatgagcact gactgcacta gacatgtagt agctaaaaga
    22321 aacaaaatca acagggtagg ctgctaagat ttcttcatca cctacttcta tttttaagtt
    22381 gacacctttt tcttcaaaca ttcgttgcgt atacgctgga taatatcctc ttgcttgaac
    22441 atcactaaag aacaaagaag ttctttgctg ctcaattgtt ttttgaatat catccggatc
    22501 acaagaatac ggaacaagaa gctgatagct gatcatacaa ccgacttgtg aacctttgat
    22561 aatttcatga caagcttttg tagctaaagc acttgctata aactgatggt gtgcaccttg
    22621 gtatttcgct tgttccaaat ggggatggtt ttcttcaatt actcctacgc taacaaaagg
    22681 gtgatgtttt acacagttga tctcattgaa agtcatccaa tattttactt tatttttata
    22741 gcgtttgaat actgtttcag cgaaacgaac gtaaaactga ataagctttc gattaggcca
    22801 tccaccatat tctgtaacca aatataacgg catttcataa tgggaaagag aaacgactgg
    22861 ctcaatccca tattttagac attcgtcaaa cacttgatca taaaaaaata atcccgcttc
    22921 attgggtgtt tcttcatcac catgaggaaa aattcttgtc catgcgatag acatccgata
    22981 gcatttaaaa cccatttcag caaataaacg gatgtcttct ttaaagtgat gatagaaatc
    23041 atttccgtgt cttttaggat aataatactt gctgttggga tcctgcgccg cttttacttg
    23101 ctgatgcgtc atcatatgcc attgatctgt ccatttttct ttaggtaaat cgctgtcgaa
    23161 tgtatacaca tcagacacag ataatccttt ttcatctaca tcccatgcac cttcacattg
    23221 attggccgct gtcgctccgc cccataaaaa tccttctgga aattttttca tatcgatccc
    23281 cctaaacttg ctagttttct tgtttgatta tttttcttat gactagtaat gatatttttg
    23341 aatctactac tatttgtaac aataaccatt gtggtcggat catatccctc tttgattaat
    23401 tcttgcaaat ctacttccat caataaatcg cctttacgta ctttttttcc atcagatact
    23461 gcactttgaa agtatttgcc atttaaattc actgtatcaa taccgatatg gatcagtatc
    23521 tctgctcctt cttcactgac taccccaatt gcatgtttcg ttggaaaaac tgttgaaact
    23581 tccccgttta caggactgta gatttttccg tccgttggaa tgatcgcaaa cccttctcct
    23641 aaagatccac tagcaaatgt tggatcgttt acttcagata aatcaattat ttcacctgct
    23701 gcaggagtac ttaacactat atcctttgag gattcttctt cgatttcatc cttgtagccg
    23761 aaaagaaaag ttagcataca ggaactgatc atcgctaaga aataactagc aactagccaa
    23821 acaaatcctt caccaatata aatcgggaaa gacaaaatac ttcttgtagc aactgccaaa
    23881 gcaccggctc ctgcggcacc agctactcca ccaaatatag ctccacatat acaagcaatg
    23941 taaaatggtc gtttatattt aagagtcaca ccataaacag caggttcagt gattccaaat
    24001 aatgctgtga taaatgcaga taaagataaa ctttttactt tcttattttt cgttttcaaa
    24061 aataccccaa gtactgctcc tgcttgcgca gctacggcag tcatacatgc tggacctagc
    24121 gtatcacgac cgtagaaaga aagattgttg gtcattactg gtacaagtcc ccaatgtaac
    24181 ccaaaaatca ctaacacttg ccaaaatccg ccgataacag caccagataa aacagggcta
    24241 aagttataaa cagctaaata tccagctgca atcagctgac ttgcataatc tgcaattgga
    24301 cctacaacta ataaagtaat tgggaccatc acgatgatac aaatgaatgg tgtcaataaa
    24361 tttcgaatac tttcgtgcaa gcttttgcga atcttaggct caatataaga aagaacccat
    24421 acagctaaaa ttgctggaat aaccgtactt gtatatcttg ctagaatcac gggcatccct
    24481 agaaatcgca aggttatgct atcactatat gcactgacaa tcgtaggata aacgagagct
    24541 gctgcaataa ccatagcgat gaaacgatct gtattgaact tttttgcagc agtatatgcc
    24601 aaaaatatag gcaagaaata gaaaaatgca tcagctgctg cattcaaaat gatataagtt
    24661 cctgattcag tagttaataa attcaaactc gtacataagg cgagtactcc tttgatcaaa
    24721 ccacttcctg ccaagacgcc taaagtcggg gcaaaaacag cggcgatcat gtctataaaa
    24781 gagtttaaaa tgcctttttt ctcagttcct ctctttccac tagatgcatc atcttctagt
    24841 ttcatttgtt tggataatac gtcatacact tcatttactg catttcctac tacgacttga
    24901 aacattcctc cagcttcgac tacagtgatc acaccttcta gttgctctac cttctcctta
    24961 tctgccttct tattatcttt tagggtaaag cgtaaccttg ttgcacaatg gtaaacattg
    25021 ctgacatttt cttcgcctcc gacaaatcgg acaatatctt tcgctaattg tgaatagtcc
    25081 atttttactt cccccttgtt aattaatgcc gtagcaatcc ttcttacaag ataagtatag
    25141 ttcttagaaa acggtttctc cattcaatta gtaccccttc taatagcgaa cgaaactgta
    25201 caatagtgtg tatggtttac ttagccgagc aaatcactca ctgcttgaaa gaaaacaagt
    25261 atatcgtttc tcttatagtc aaaggagaca aaaaaaacac gcttgatctt cctttgctga
    25321 tgtataagtg gtatctcaag ctgatactct gtgatttttg tttctacttg gcttttgata
    25381 agacagcaca acaaagtctc ggcatttaac ggaagcataa ctaggtcaga actaggagag
    25441 attagttgct gaatggaatc tatcttttta tggattttta tttgattttc ttctttgtag
    25501 taatgatcaa gcagttttgt tttctttttt tcttcaacta tggtcagctt ttcacgcaaa
    25561 atctgataaa tctccgaaga ttcaaactca tctagaacac taggtagata aatcaaatca
    25621 tcccactcaa aaataagctc ttgctcagta gtcaaaattg catcatattc cgttattttg
    25681 tttgattgat ttttgaaaag atacactggt tctatccggc attctttgat ccacatgttc
    25741 atactttgtt gaatctgttt ttttagtgta ttcaaaatac taattggctg gttactaaca
    25801 atcagtatct ccccttgttt tcgaaaagga ttcaacgctt cggcaagata caaaaccaaa
    25861 taagcgcttt cagctaaatt gggacgtaat tgaaaatgta ttgggaagaa acgccgaatt
    25921 aaataaattt ctaaaggata acgggagatg atcttttgta caaaatgatt catggtatta
    25981 tgtccggcat ccatcctcgt tttcatttgt ttgatatggg ttagcaaaaa ttctgtttca
    26041 tggaacaata acgaaacagg aagatctaaa aaacgaataa tcgtttgctc aaatgctctt
    26101 agattcttct ctatttcttt gtttttttca tcattaatca tcaaataatt taactctaat
    26161 agtcgatttt caattgcttg tttttcttga ttggaaaaag aatagggtag ttctgatgat
    26221 aacttttttg tgagggtctc taccatcgga ataaataatg tatacctgtt tgttttttct
    26281 aaaagttctt ctcttcgaag aatggtcaaa acgagatacc gagaaaatcg tatgaagtcc
    26341 tctcctgaaa caatataatt atatgatata agaacctctt ttaatgtctt ttggacagtc
    26401 ggcatgatat tggaaaaaat tgtactgatt tgttgaggca accttgcgtg ttggatcacc
    26461 gtttctgttc caacaagaga taagaaaatt ctttttgaat tttcttcagc aatgattttt
    26521 agtccttttg tcgacgaaac ttccagttct aatttaggat tgcgctcgat ccaacgcaaa
    26581 atcgttttta tttgtaatga tacggctgtt tttgaaagaa agaaaatgtc tgctatcttt
    26641 tgcatactga catacccttt ttgaaacaat aagtacgtca caatagttga agctcggaaa
    26701 tccatgctgg aatagatttt atgtttcgtc acctcttgta tgatttgctg tttagtttca
    26761 gattcaattg attctaaaaa ataacctgaa cgtttatttg actggataat gattttcttc
    26821 tcgtagatag caagcatatt tttgatttca gtttggatcg ttttcttgtt ggtacctaaa
    26881 gcttcagcta attcttcgct tgttatccaa tgattcttat ttcttaatac tacaagaatt
    26941 tggagttgtc gtttatttaa gtccatttgc ttacacctcc tctttgttat aaacatacag
    27001 gaaaaacctc tcaaatgaca atagtcactt gcagaggttt aaaaatcatt tgaagaaaga
    27061 tctatattca aggtttttac cattagactg gataatttgt ttataatatt caaaactatc
    27121 ttttttcaat ctcctaaagc ttccgctccc atcattttgt ctatccacat aaataaagcc
    27181 atatcgcttc ctcatttccc ctgttcctgc cgatacgata tcgattggtc cccaccataa
    27241 gtaaccaatg atttggcaac catcttttat tgcctcagca atttcctgaa gatgtatttt
    27301 tagatactca caacgttttg gatcgtggat tcgattcaat tcatctggtg cttcatctag
    27361 tccaatgcca ttttcaacta tgaataatgg taattcataa cggtctgtca attcattcat
    27421 gacataacga aaccctagag gatcaattgg ccaactccaa ggttcgggag atttttcctt
    27481 caaaaaggga ttatcgaaac catttgctcc gccagtatct acgcgcattt ctgcttcttt
    27541 tttatagaca gcgctacgat aataactaaa tgcaatataa tctaccgtgt tagcttgcaa
    27601 gtactgttcg tctccatcta acattactgg tgcacagtct tcttccttcc atatcctgta
    27661 aatataagct ggataatgcc ctcggcacat gacatcggag aaaaaccatg aattgcgttt
    27721 aaattgcagt gaaccaaata cattctctgg atcacagtca tatgcataag tcgctaatgc
    27781 agaaaagcta cacataatac ccatcttagc atttggatcg attttcttta ataattgagt
    27841 agcacgggcg ttcgctagaa acatatgatg acaggcttga taaatctctt tttttgttag
    27901 tgtagcaatg ggttcaagtt tatctctagc agggaatgta accaatccag cagctgcata
    27961 aggcatacga aaagcattat tgatctcatt aaaggtcatc caatatttca ctttcccttg
    28021 gtagcgttca aagacggtct gcgcataaaa ttcaaaaaag tcgattaatt ttcgattttg
    28081 ccacccccca tactctccta ccaatgctaa gggggtttca taatgactaa gagtaattac
    28141 tggctcgata tcatatcgat ttaatgtatt gattagtctg tcataaaata cgagaccagc
    28201 ctcatttggc ttctgttcgt ctcctcttgg aaatattcgt gcccatgaga tgcttgttcg
    28261 aaaagctttt aaacctaact ccttcagtaa acgaatatcc tcttcaaatc gatgataaaa
    28321 atcaatcgct tcatgagaaa gataatggag tgattcgtct aaagcaatct cccaaatctt
    28381 tttttcttca ttccactgaa tactaggatg ctggttcatg atcccgataa gcgtatctgt
    28441 cacattaggt aatttgccgt cttccaacca tgcaccttca cattgattag cagctactgc
    28501 tccgccccat aaaaaatttt ctggaaatat ttcctgatcc ataactatct ctcctcgcta
    28561 tcaacaattt cttacttgaa gtatagctcg attcatcaaa acaagatatt ctaactattt
    28621 cggcagaaga gaagaaaaaa attgaatgct actcttcatt tttaatattt atatataaca
    28681 aatacataag acgatccccg aaagaaaaca ctcgttaacc atctaagcaa acgatttatt
    28741 ttacttccaa cacaaatgct tcataaggtc gtaaatcaac tttgccgtct ggtaactgtt
    28801 tcagaggata atttgacaca ataaccctta aagcttcacg tggaacagtg aacgtctgct
    28861 tttcactgga catattacaa ataacgagcc attgctggtc ttcatatatg cgagaatagg
    28921 caaagacatg ctcaggcgtc ttttccacta attcatagtc tccccagacg atgatgtcat
    28981 gttttctacg caaatcgatc aatttcttgt acatataaaa aacagaatct tgatctgcta
    29041 acgcttcttc tgcattgatt tctttataat ttggattgac tcgaagccat ggaattccag
    29101 tagtaaaccc agcatgttca cgggcgttcc attgcatagg cgtcctagca ttgtctcttc
    29161 ctttcgcatt gattgattcc aagatttcct cttttgtaaa tccagaagaa atcctttcgt
    29221 tatacatatt gattgtctca atatcttttg cttccgcgat atcatcgatt gggcaattcg
    29281 tcatgcctaa ctcttctcct tgatagatat aaggggtgcc tttcatcata tggagcaaga
    29341 tagcaaacat cttggcactt tcttttcgat acacatcgtc atttccccaa cgagaaacaa
    29401 ttcgcggaag atcgtgatta ttccaaaata aactattcca tccttcatca cctaaagatg
    29461 tttgccattt ggataatact tttttcagtt cagagatact caagggtttc aaatcccatt
    29521 tgtctttgcc ttcttgctga tctaatccta tgtgttcgaa ttggaagacc atggataatt
    29581 catttctttt aggattggaa taaagtttag cgatttcggt agttgctccc catgtttcgc
    29641 caacagtcat cagatttttg tcaccaaaag ttgcttgatt catttcctga agatactcat
    29701 gcagtttagg tccattactt gtgatttctt tatccggttc tttgccaatc atatcgatca
    29761 cgtccatacg aaagccacca atacctttat cgatccagaa attcatcatt tgataaatct
    29821 cttggcgaac ctgtttattt ttccaattca aatcaggttg acgcttgcta tacaagtgca
    29881 gaaaatattg ccccgttttt tcatccaact gccaagcaga accactgaat attgagcgca
    29941 atctattagg ctcttttcca ttgaccggat cacgccaaat ataatagtcc cgataaggat
    30001 tttctttatt tttacgggcc tcaatgaacc acggatgttc atcagaagta tgattcacca
    30061 ccagatccat gatgatacgg atatttcttt tctttgcttc ttcaatcagt tcttccattt
    30121 cttccatcgt accgaattct gccatgattg cttgataatt actgatatca tacccattgt
    30181 catcattcgg agactcgtat actggagaaa gccaaattgc atcaatacct aaatacgcca
    30241 gataatccaa cctggaaata atcccccgca aatctccaat accatcttga tttgaatctt
    30301 gaaaacttct tggatagatt tgataaacaa ctgaatggtg ccaccatttt tgttccatga
    30361 ttttcctcct ataaatggat gtttctattt tttgcttaat catttatttt agtctctctc
    30421 actaaaaatt gtaacgcttt caaatagcta ttgcaccttg ttatcggtaa cagaataaaa
    30481 caaaaaaaca gctgaggaaa ttccctcaac tgttttttga aaggattgat ttattcaggg
    30541 aagaatgctg atttcacaac ttctttataa acggtgttca ctcagctgtc cctcggtatt
    30601 agctcaaaaa acggcaaaat ataagaaact atttttttcg tttttttctc taatactcag
    30661 gccagaacac tgatttcaca acctctttat aaacggtgtt cactcagctg tcccttggta
    30721 ttagctcaaa aaacggcaaa atatgaggaa ctattttttc gtttttttct ctaatactca
    30781 ggccagaaca ctgatttcac aacctcttta tttcacattc aatatccaca tgaatcccat
    30841 gaatacaaag aacagaccat acatgatggg agatacttct tttcctcttt tagctgctaa
    30901 catggtaatt ggatagaaga taaaacctaa tgcaatccca tctgaaatac tataagtcaa
    30961 aggcatgccg atcaagatca agaatgaagg gatcgcaatc tctaagtttc cccattcgat
    31021 ttgtcgcaaa gattgtgcca tcaatactcc taccataatc aatgctggtg cagttacttg
    31081 tgaagtaaca actgatagta atggagaaaa gaacaagcct aacaagaaga aaatccctgt
    31141 agtaatagct gtgatacctg aacgtcctcc aacggcgata ccagctgatg attctacaaa
    31201 tgcaccaaca ggagaagtac ctaataagga acccgcaagc atagctgttg aatcagaagc
    31261 taaagctttc cctacacgag gcatcttgtt gtctttcatg aaacctgctt ggtttgctaa
    31321 tccaactaaa gttcctgctg tatcaaagaa agtcacaagc aagaatgtca gcactacgac
    31381 ccacatttga agagaattga tgtcccctac atgttttaat gcaactaaaa atgttggttt
    31441 caaacttggt gctgcggcaa caatttcaga aggcattgga atcaaacctg tgaccagacc
    31501 taaaatcgtt gtagctgcca tcccgatgaa gatgccacct ggtacacgcc gaacaagcaa
    31561 gatagcagtt acgactaatc caaaaatcgt caaccaagta gaaccaacgt tgagtgatcc
    31621 taatcctact aatgtagatt cattcgaaac gatgatccca ccttcgctca gaccaaggaa
    31681 agcaataaat agaccgatac cgccagaaat cgcatatttc aaatctgctg ggatcgcatc
    31741 aatgatcatt tcccgcagct tgaagattgt gatcaaaata aaaatcaaag aagcaacgaa
    31801 tacgccggct aatgctgttt cccatggaat ccccattcct aaacaaacag aataagcaaa
    31861 aaaagcattg atcccaagag caggtgccgt tccaattgga taacgggcta atacccccat
    31921 taaaatacag ccgatcgcac tggctaatgc agtcgcagta aacactgctc cttcatccat
    31981 tccagaagca cctaatacag ttggatttac aaataagata taagccattg agataaatgt
    32041 tgtaaatcct gccagtacct cacgttttac cgtggtgttc aatttttcta tttcgaagta
    32101 ggaaagtatt ttttccttca tcttccatcc ccctaaatcc gaacattata aataaagtcc
    32161 gttaaaattt cgatttatta ataaaacaac gtgttttatc atatactaaa acaaaacata
    32221 agtaaacaaa aaattcggat tttaagcaaa atccgaacgt tccatctgat taaatccctt
    32281 tatctcaaag ttcatgaaca ttcactttgg attacaagaa tttctttatt gtgttaataa
    32341 agtttcttca ttctagtctc aaaaatcaat agtgtgattt gattttacca tttcgtttct
    32401 gttcttcttt gatgaattct tttgtatcga caggattcag attatttagt ccgccaaaac
    32461 ctgtatcgac catttttccc cagccttttg tcttcttttt tcctgatttg agattctttg
    32521 ctcgaagctt ctcactatgt tttgctcgcc gatacttcaa ataaccttct aatcttccca
    32581 ttagatccac tgtttctcct cctttttatt caatcagacg actcagcttt tcctataaca
    32641 aaataattgc cttcagggtc cgaaaaattg aaagtcaaga cgccattgtt gtctgtgatt
    32701 tctcctggtt cttccatgtt ctcgtagata gtttcaaatt catccgtaaa aaatactaaa
    32761 gaaggcggtg gtcctaatac ctctggtgaa aacttctgca caaatacttt tgggaatacg
    32821 cctaattcta attcttttga gatgctgagt acaatactct taaattcttc tggcaattcg
    32881 attgtttcag aaatttctgc accaattttt tctacaaaga atcggcaaat catctctacg
    32941 tcattgacat aaagcatcac acgtgttttt tgtatcatcg tttgaccctt ctttctattt
    33001 gattttcttt cctgtctata aactgattcc attatatcac gaaagcgcaa acaccttgtg
    33061 tttgatacta ttcaagaaaa ataaagcaat agtttctagg attgctttcc tccacaaact
    33121 tttttta
//
LOCUS       DAZABD010000029        32759 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000029 DAZABD010000000
VERSION     DAZABD010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32759
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.029"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..553
                     /locus_tag="ACHALG_001404"
     CDS             <1..553
                     /locus_tag="ACHALG_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFN2586823.1"
                     /translation="GIGVDIDDTLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFA
                     ISDLPIGYYPNDEKAKEFLTSTEEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLET
                     DQSEEIKKWFKEEGYLQGMIQLPDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLA
                     SLKEPEKVTEFFNEFKNWKSSAL"
     gene            578..1762
                     /locus_tag="ACHALG_001405"
     CDS             578..1762
                     /locus_tag="ACHALG_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HFN2586824.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(2199..3782)
                     /gene="abc-f"
                     /locus_tag="ACHALG_001406"
     CDS             complement(2199..3782)
                     /gene="abc-f"
                     /locus_tag="ACHALG_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protection-like ABC-F family protein"
                     /protein_id="HFN2586825.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(3887..4015)
                     /locus_tag="ACHALG_001407"
     CDS             complement(3887..4015)
                     /locus_tag="ACHALG_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2586826.1"
                     /translation="MMRMTKITSNENYQRYHIAFSHAIGFTVAVDGTSLSKTVEFT"
     gene            complement(4360..4503)
                     /locus_tag="ACHALG_001408"
     CDS             complement(4360..4503)
                     /locus_tag="ACHALG_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFN2586827.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(4726..5205)
                     /locus_tag="ACHALG_001409"
     CDS             complement(4726..5205)
                     /locus_tag="ACHALG_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFN2586828.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(5278..5562)
                     /locus_tag="ACHALG_001410"
     CDS             complement(5278..5562)
                     /locus_tag="ACHALG_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2586829.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(5640..5834)
                     /gene="ssrS"
                     /locus_tag="ACHALG_001411"
     ncRNA           complement(5640..5834)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHALG_001411"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(5906..7192)
                     /locus_tag="ACHALG_001412"
     CDS             complement(5906..7192)
                     /locus_tag="ACHALG_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFN2586830.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            7330..8001
                     /locus_tag="ACHALG_001413"
     CDS             7330..8001
                     /locus_tag="ACHALG_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HFN2586831.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            7967..8455
                     /locus_tag="ACHALG_001414"
     CDS             7967..8455
                     /locus_tag="ACHALG_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HFN2586832.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            8468..9415
                     /gene="prmA"
                     /locus_tag="ACHALG_001415"
     CDS             8468..9415
                     /gene="prmA"
                     /locus_tag="ACHALG_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFN2586833.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            9416..10180
                     /locus_tag="ACHALG_001416"
     CDS             9416..10180
                     /locus_tag="ACHALG_001416"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFN2586834.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            10413..12626
                     /locus_tag="ACHALG_001417"
     CDS             10413..12626
                     /locus_tag="ACHALG_001417"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFN2586835.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            12637..13083
                     /gene="dtd"
                     /locus_tag="ACHALG_001418"
     CDS             12637..13083
                     /gene="dtd"
                     /locus_tag="ACHALG_001418"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFN2586836.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            13254..14513
                     /locus_tag="ACHALG_001419"
     CDS             13254..14513
                     /locus_tag="ACHALG_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HFN2586837.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(14602..15549)
                     /locus_tag="ACHALG_001420"
     CDS             complement(14602..15549)
                     /locus_tag="ACHALG_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFN2586838.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            15943..17235
                     /gene="hisS"
                     /locus_tag="ACHALG_001421"
     CDS             15943..17235
                     /gene="hisS"
                     /locus_tag="ACHALG_001421"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFN2586839.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            17250..19019
                     /gene="aspS"
                     /locus_tag="ACHALG_001422"
     CDS             17250..19019
                     /gene="aspS"
                     /locus_tag="ACHALG_001422"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFN2586840.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            19128..20003
                     /locus_tag="ACHALG_001423"
     CDS             19128..20003
                     /locus_tag="ACHALG_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFN2586841.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            20269..22152
                     /locus_tag="ACHALG_001424"
     CDS             20269..22152
                     /locus_tag="ACHALG_001424"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFN2586842.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(22227..22511)
                     /locus_tag="ACHALG_001425"
     CDS             complement(22227..22511)
                     /locus_tag="ACHALG_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFN2586843.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(22501..23235)
                     /locus_tag="ACHALG_001426"
     CDS             complement(22501..23235)
                     /locus_tag="ACHALG_001426"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HFN2586844.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            23364..23987
                     /locus_tag="ACHALG_001427"
     CDS             23364..23987
                     /locus_tag="ACHALG_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFN2586845.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            24179..25300
                     /locus_tag="ACHALG_001428"
     CDS             24179..25300
                     /locus_tag="ACHALG_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFN2586846.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            25307..28435
                     /locus_tag="ACHALG_001429"
     CDS             25307..28435
                     /locus_tag="ACHALG_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFN2586847.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            28454..31648
                     /locus_tag="ACHALG_001430"
     CDS             28454..31648
                     /locus_tag="ACHALG_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HFN2586848.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(31794..32501)
                     /locus_tag="ACHALG_001431"
     CDS             complement(31794..32501)
                     /locus_tag="ACHALG_001431"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFN2586849.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMLPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        10700 a         5905 c         6970 g         9184 t
ORIGIN      
        1 aggaattggt gtggatattg atgatacatt gttggctgtc gcagcttcta caagtgattt
       61 gacacaagca aatgttcaat actttcatca agacgggttg caggaattat taatagatcc
      121 tgtcgatttt gcaatcagcg atttgccaat cggttattat ccgaatgacg aaaaagcaaa
      181 agagttttta acaagtaccg aagaaggcca tagttatgca catcatttat tgctagagca
      241 atcaatgaaa tatgtgaagc cggatggttt tggtttgttt ttgatgccga gtggattttt
      301 agaaacggat caaagtgaag aaatcaaaaa atggtttaaa gaagaaggtt atcttcaagg
      361 aatgattcaa ttgccagatg aattgttccg taataaacaa tcgcaaaaaa gtattttgat
      421 cttacagaaa aaaggaccac aagcacaaca agtaaaagaa gtcttgcttg tgaaacttgc
      481 ttcattaaaa gaaccagaga aggtgacgga atttttcaac gagtttaaaa actggaaatc
      541 atctgctctg taatcattca acgagaagga gagtcatatg tctaaaacaa ttgcaatcaa
      601 tgctgggagt tcaagtttga aatggcaatt atatcaaatg ccagaagaaa ccgtagttgc
      661 gaaagggatc gtcgaacgta tcggtttaca agattctatt tttaccatca agtacggcga
      721 taatcaaaaa ttcgaacaaa tcatggatat cgaaaaccac gaagtagcag taaaaatgct
      781 gttagatcag ttgattgaac tgcaaatttt aagctcttat gatgaaatca ccggagtagg
      841 gcaccgtgtt gttcacggag gagaaactta tggggattca gtagtgatcg atgagaaagt
      901 aatggaccgc attgctgaat tagcagaatt tgctcctttg cataatcctg caaacttaat
      961 ggggatcaaa gcgtttaaaa agattttacc tgacattttg agtgtggctg tattcgatac
     1021 atctttccac tcaacaatgc caaagcaaaa ttatttatat agcttaccgg tgtcttatta
     1081 tgaagatttc aaagtaagaa aatatggctt ccatggcaca agtcatcgtt atgtgtcgga
     1141 acgtgcggca aaattattga acaaaccgat cgaagaacta aaaatcatca cttgccactt
     1201 aggaaatggt gtatcgatca cagcagtcga tggaggtaaa tccatggata cttctatggg
     1261 attcacacca ttagctgggg tgacaatggg tacacgttca ggtgatatcg atccagctgt
     1321 tctcccatat ttaatggaaa aattagagat tgatattgat gaaatgatca atgttttgaa
     1381 caagaaatct ggtctgctag gcctaacagg attgtctagt gatatgcgtg atcttgaaaa
     1441 gaactatgaa aaagaacata ttcgtttagc ttatgacatt ttcgtggacc gcatccgaaa
     1501 atacatcggc ggttatgtga caacgatgaa tggtgttgat gcaattgtct ttactgctgg
     1561 tatcggcgag aatgacggac atgtacgtag tgaaatcatc aaaggtatgt cttggtttgg
     1621 ttgtgaaatc gatccagcgt tgaatgaatt gcgtggtgaa gaagtagata tctctacaaa
     1681 agaatcaaaa gtaagagtat tagtgatccc aacagatgag gaactaatga ttgcccgtga
     1741 tgtggagcgt ctgcgcggct aagccaaagc attcgttagg ataaaagatg aaacgcatgc
     1801 aagagggaat tcttgcatgt gttttttttg cggagaagac cgcaaaatgg taatttaact
     1861 tcaatttccc ttctttttta caggatcttc ttagggaatt ttgcttggaa aagtggtcaa
     1921 aagggaaaag actggcattt tatttattga ttttcaggaa aatcccaata ttttattttc
     1981 attcatcttt ataagggaca ttacatatgt aaaaagaaaa aacattttta gatcagcctc
     2041 gtgggctgtc gactttattc ttcgctttat gtttagctag aaaaccgatc cgaaacattc
     2101 attgatcgaa taataaaaac ttgattttcc acctgtactt tgcctgaatc gtgaaagcac
     2161 aggtatgaaa atcaagtttt ttgttgcgaa aaaacgtcct agtcaatcag ttggccgagc
     2221 ggctggcggc tgatctgctc ctgatcaata acgagtgctt cttggaagtg ttgctgcaaa
     2281 aagtagcggt catggctgac tgctaggagc gttccttgat agtcttgcag catttcttca
     2341 atttcttctc gagcatagct gtcgaggtga ttggttggtt catccaaaat caggacgttg
     2401 attttttcta aaaagagttt cagcaacgcc aaacggattt tttcaccacc ggataaatct
     2461 ttgatccgtt tacccacatc gctttgataa aagccacttt tggctaattg ccgtcgagcg
     2521 acttcttcgt ttccagtcaa tgtgatggcg taagccagca aacggctatc gggatcggca
     2581 aactgcagct gctgcggcaa ataaccgatt tgcaagctac tacctaattt gatggtgcca
     2641 ccgtaaggct tttcctcacc gagtaataat ttgagtaagg ttgattttcc tgcaccattg
     2701 tcgccgatga tggctaaatg atctccacga tagactgcaa aactgctgtc tttgaatagt
     2761 acttgatcag cgtactgctg gtgcaagttt tgaatgatga aaacttcttt gccagatttt
     2821 ccaccattag acaagctttg cagccgattt ttcgggggag gtggcggttt gacgagtgtc
     2881 agtttcgcca ggcggcgttc cagttctttg gcttttttga agaaggactc attgtctcct
     2941 tcatgtgccc actgacgaaa acgacgaatc atgactttca accgctggac ttctttttgc
     3001 tgcagttcgt aattttttgt cagctcagcc aagcgagctt ctttcaagac tcgaaaacgg
     3061 ctgtagtttc cgggatactc aatcaatgca ccttcttcca gttcccatgt ttttgtggtg
     3121 acttgatcga gaaattgccg atcatgagaa acgaccagaa ccgatttttt tgtattctgt
     3181 aaatagtttt ccaaccagcg aatactgtta agatctaaat gattggtcgg ttcatcgagt
     3241 aaaagtaaat cggcatcggc tgctaaaatt ttggctaaag cgactttaac tcgttcgcct
     3301 ccgctaagtt ctgttaaccg tgcttcctga cggtgtggaa tccctaagcc ttgcaagatc
     3361 gctgcaatcc ggtcttctaa tacgtaaccg cctagttctt caaagcgatg ctgcagctca
     3421 ccgtaacggg ccagacactt ctctaagttt gtatctggtt ctgtcatcaa tgcttcgtag
     3481 cgactcattt gctgtttgag gtcctgtatc tcagcggcag aatggagcaa gtagtggttg
     3541 acggtcgctt cggaaactgt aagttcttgt tcgaccgtcc cgatctttaa gccttttttg
     3601 cgactgataa caccgctgtc gacaccttct ctgccggtca agatttgtaa aaaggttgat
     3661 ttgccagttc cgttgatacc gactaaggca attttttcac cagcttcaat ttgacaggat
     3721 aaatcctcaa ataatggatt cccgccgatt gtttttttaa tagaaattgc ttgtatgatc
     3781 atgatctctc tccttgagac cactcaaaaa ggccatggtt atccatggcc tgtgttattt
     3841 aaactcagat tatcatgaga aaaatacctt gagatggcca ttgattttac gtgaattcaa
     3901 ctgttttgga caaacttgtc ccgtcaacag caactgtaaa accaattgca tggctaaatg
     3961 cgatatggta tcgttggtaa ttttcattac ttgttatttt agtcatgcgc atcatagtat
     4021 tttccctcct tcgtgtagtt gtcttttcca gtgtacctga aagtgtcaga aagttcaact
     4081 agcgctgaca tttacttaat atttatcgat gtgtaattct gtcagagagc tttttttatc
     4141 tgactgaggg taatgtctcg tggaccacat cattgtattc tgagctaaac cagcgttcag
     4201 gcgaaatcat atggtgtttg ataaaaaact ttgtctacat gacttccttt tgccatttgc
     4261 ttaataccgt tgcattaaac agctcttctc tttggaaatc tcaccaacat cgcgttcagg
     4321 agtatcgttg tggttgcaat ggcaagcggt agcctttcat caattcttga atgatttacc
     4381 gaatgtagca cttgccttct cgcggtaccc gaacccgaaa taaaatttta gtttggcgat
     4441 aaaatcggta tccgccatcg atacttcata ttttttcgta tcagattcat cggaaacttc
     4501 cacattgacg aaccaataag ctttggcatg cttaggtcgt tttaaaccac accagggcga
     4561 ctaatcgccc tggtgttcac ttttttttat tttacttgaa ctcattgact gttttccctt
     4621 tattttaaac attataaaga ccgatgagtg cttgccgcgt cttttgtttt tatcacggtt
     4681 ttttgtgcaa aaaaacctgc cgatagttgg caggtcgtct acgtgttaag ctttggtgtg
     4741 gtctttagct ggcaattggt tttctaaatc agttcggaca acaagcacat cgcaagctgc
     4801 gtttctaata acatattcag aaacagaacc aataaataaa cgttctacag cattcaatcc
     4861 tgtggctcct aacataatca aatctacttg attatcttct gggatttctc tggcaataat
     4921 ttgttttggt gagccatatt ctacgacaga ggttacatta ttcaagccag ctttcttcgc
     4981 attgctttca taatctgcca atgtttgctt tgccatttca gtagcttgtt ctgcaagcat
     5041 tccatcaaag gatgaaacgg tttggaaaga acgtgtgtca atgacatgtg ctaatagaag
     5101 ttctccgtta tttcggatag ctacattgac cgccttttta aaagctaatt cagcttcatc
     5161 agaaccatct acggcaacca tgattttttt gtattgttgt aacataagca ttcactcctc
     5221 aagtcaatcg tattgtactc ttttattgta gaacacttta gggaaaaaga gaattattta
     5281 ctgctttggt tcagcaattt tttttgcaaa tcggatactg aaggtggcag aaacaataat
     5341 tacgatcagt aaaaagacca aggcaataaa gcgttgattg aaaaaagctg ttaaaatacc
     5401 cattacgcct aaaacagcat atgcagctcc gtaaaattgt aggaatcctt gatttttatc
     5461 atttttgttg atcaatacaa aaaagatatc ctgtttatta aataaataat agctaacgaa
     5521 taataatact aaagtggcga taatcattaa aatcctaatc atgaacaaat cctccaaaat
     5581 aagcaacaaa tagaaaaagc aatttgccaa gagcaaattg ctttattctt taaaagatgg
     5641 taatctccgt taatgccgtt gtttgtcctg tagtattgat cccgcgaatt caagcaggtg
     5701 ggtcccatgg tcgtagcttc gaactaccaa atgataaggc atgttaccag cagtgacaca
     5761 agacgggtcc caagatatca ataaaaatgt tcggtcaaca cacaaagagg acaattcgaa
     5821 catcacagat ttcccacctc tatagtagca tatgaattta aaaagagcaa tgagagagct
     5881 cgcagaaaca gaaccgtttt cttgtctagt tttttggagg atgctttttc cactctttta
     5941 tacggtaata ctgttgaccc agagcctgtt catatttccc tgtatctttt ggctgataat
     6001 actgagcatt cttcagttta tctggtaaat attgctgatt gacccaagcc tgatcaaaat
     6061 gatgaggata ctgataaccc acgccacggt tgagtgattt cgcacctttg taatggctat
     6121 ctctcaaatg atcaggaaca tctccagctt tgccctctcg aatatcagca agtgctgcat
     6181 ctaatgccat gtaggcagaa ttggattttg gcgacaagca aagatcgacg acgacgtctg
     6241 ctagtgggat acgtgcctca ggcaatccca atttttcagc agctaataca gcgttcactg
     6301 tacgagccgc agcagcagga ttacctaaac ctatatcttc ataaccgata accatcagac
     6361 gccggcaaat acttgccaga tcacctgcct caactaaacg ggcgagataa tgaagtgcag
     6421 catccacatc acttccacga atcgattttt gaaaagccga aataacgtca taatgagcat
     6481 ctccattttt gtcatgggta agagcttttc tttggacaca ttcttcaatg atcgacagtg
     6541 taagatgaat ccttccttct ttatctgaag gagtcgatag tgtagccagt tccagcccat
     6601 tcaatgcact ccgcaaatcg ccgttcgttg cacgcgaaag gtgaagaaga gcatcttcgt
     6661 ctaactggat tgcttgttgt ccaaggccgc gttctttatc ccttaaagca tgttcgactg
     6721 ccaactggat gtcttgttct gttaatggtt tgacttcaaa gatctgtgta cggctgcgga
     6781 tcgctggatt gatggtaata taaggattct cagtagtcgc tccgatcaag atgattcggc
     6841 cactttctaa atgaggaagc agaaaatcct gttttgtttt atctaatcgg tgtacttcat
     6901 ccaataagag gatcactgta ccactcattt ttgcttcttc cgctacaact tgtagatcct
     6961 tttttgaatc tgtagcggcg tttagcatgc gaaatgcata attagtagag ccggcgatcg
     7021 cacttgcgat actcgttttt ccagtaccag gaggtccata tagaatcatg gaagagagca
     7081 tgcgagcatc gaccatgcgg cgtatgattt ttccttcccc gactagatgc tgctgaccaa
     7141 cgacttcatc gatcgttcga ggtctcatgc gataagccaa tggctgttgc ataggatttt
     7201 cctccttctt gtttttacgt cccttaatta tatcatattt agcaagatgc ctagaaagaa
     7261 cagccttctg ttgttttgtt ttcatagctt atccaactaa tattgtgtat gataaagtag
     7321 gtgagaaaaa tgacggataa acaaaagata gatgaatttt tttggaataa aacgacgcca
     7381 gaagtggcac gggatttatt ggggatgtat ttggagcatg acacaccaga aggaagatta
     7441 gctggatata ttgtagacgc ggaagcatac ctaggtccag aagacgaggc cgcccatagc
     7501 tatggtttaa gaagaacacc gcgtgtgcgg gcaatgtatg aaaaaccagg tacgatctac
     7561 ctttacacga tgcatactca tcggattttg aacatcatta ctcagccaga agggattcct
     7621 caaggagtaa tgatacgtgc aatcgaaccc gccgcaatga ttgatcaaat gagtaaaaat
     7681 cgtggcggaa aaactggtcc agatatcagc aacggtccgg gaaaattagt ggaagcatta
     7741 gcaattcctc aagaacttta tggtcagtct atagcagata gttcgttacg cttagttttt
     7801 gagaaaaaaa agacaccaaa aaagatcaat gctttgccta gaatcggtat cccaaacaaa
     7861 ggtgtatgga cagaaaagcc gttgcgtttt gttgtgtcgg gaaatcctta tatctcttta
     7921 caaagaaaaa accaaatcga gaaagattgg ggttggagga aagaaaatga aaaagaagga
     7981 tctattaaca tatttagatg aaataattga aaaacaagtg acagactatg atgtagcttt
     8041 agattgggat acaaaaaatc atacgatcga gatcgttgtt cgcttgtttg cagaaaataa
     8101 agcacaattt gagattgatg atgcagaagg gatcgtttcg caagaaccaa ttatcgaatt
     8161 tgaagacggc gtacttcttt ttaatcctca gaaatctgtt tttgatgaac gcaattatct
     8221 agcagtaatc ccttatgaag gaaaaaaagg gttggcaaaa tcagtagctg attctttagt
     8281 ggaatatcta aatgaagtat tagctcaagg gcaaagcgat ttgcttgatt ttttggatga
     8341 agacgatgac gaagctgtat tcgagcttca ctgggataat cagcaactag cagaattagt
     8401 agaagtaaaa cagcaaaatg aagcagatac ttatttaccg tacccaagtt attaaggagt
     8461 gacagatatg aaatggaatg aagtaaaagt agaaactgaa agtgaagcag tcgaagcagt
     8521 tgctaatatt ttgatggagg caggggcgag tggtgtcgcg attgaagatt ctttagatgt
     8581 tgaacatttt cagtcggatc aatatggtga gattttagat aaaaatgatt ttacatcgct
     8641 gaaagaaggc gcactagtca tggcttattt tccggaaacg actttcttac cggaaatctt
     8701 gccttttatc aaagatcgta tcacacaatt acctgagttt ggtctagcta ttggaaaaaa
     8761 taaagtaaca gttagtgaag tagaggaaag caactgggca acagcctgga agaaatacta
     8821 tcatccggtg cgtattacgc gaatgctgac ggttgcgcca agctgggaaa actaccagac
     8881 agaagatcct ctagaaaaaa ttatcacact agatcctggt atggcatttg gcacaggcac
     8941 tcatcccacg acttccttga cattgacagc actagaaaca gtcttgcgtg gtggtgaaac
     9001 attgctagat gtgggaactg ggtcgggcgt gctaagtatt gcagcaaaat atcttggtgc
     9061 aaaagatgtc tatgcctttg atttagacga agtagccgtt cgttcggcga aagaaaatat
     9121 ggatatgaat gaaatagcca aagatgtcca tgtatcagct aacgatcttt tgacaggaat
     9181 cgagattgaa agtgatgtaa tcgttgcgaa cattttagca gatatcatct tattgatgat
     9241 cccagatgct tggcgtttac tgaagcaaac aggcacgctg atcgtttcag gaatcattga
     9301 agagaagaag caaatggttc tggatgccat gttagaacaa gggtttatcg tggatcaaat
     9361 tttccagcaa aaagactggt atgcaattat tttgaaaaaa ccagaggtgg aatagatgca
     9421 gcggtatttt ttaaaagaat catatgaatc aaaagactat tttgttttag aaggggaaaa
     9481 ctatcaccac attgtacggg ttatgaggat ggaacccgag gatgaagttt ttttggcatt
     9541 caatgatcaa atagccattg ttgcagaaat cacggagatt actgaagagg ctgtttatct
     9601 gaaagaaatt aaaaaagaag aaaatcaaaa ggaactgcca agttcgatta ccatagcgag
     9661 tggctatcct aaaggggaaa agctggattg gatcgttcag aaatccactg aattaggagc
     9721 gcaagctttt cttggtttcc cggcatcagc ctctgtcgtt aaatgggatg caaaaaaacg
     9781 tatgaaacga caacaacggc ttgaaaagat tgctcaagag gcagcggaac aatcccatcg
     9841 gcagatcgtg ccatcagtag agttattgga aacgaaacaa gatttatttg agcggctaga
     9901 tgattatgac gtggtcttgg ttgcttatga ggaatcagca aagcaaggcg aaaaatcaaa
     9961 tgcagccaaa attcttagtg aaacaaaacc agatagtcga atcttagctg tctttggacc
    10021 agaaggtgga ttatcccgcg atgaagtcga ttattttgta gaacacaaag ggatactttg
    10081 tggattagga ccaagaatcc ttcgtacaga aacagcacct ctttatttat taagtgctgc
    10141 tagctatcaa tgggaattaa tgaatccttc tttcagctaa cgttcttcat taataaaaga
    10201 gtatcgacag aaagaattga aaaaatagtc aacaatttgt ataatagaaa caattattta
    10261 tacaatggat acgaaaaatc gtttgaaaaa gcttttaaaa agcgaacatt tccgtaaaaa
    10321 aacgtataac tgcacggatt ttgggacaat tatcaaacag atatttgtcc cgattcccgt
    10381 tttaccgaaa aaagaaaggg ggatcgattc ctgtgacaaa agaagaaatt ttaacgggac
    10441 ccggtgtcat aaagctcgtt agtcaataca tgggtcccga acatgtagca tttgttgaga
    10501 aagcatgcga atacgcgact gctgcacatg acggacagtt tagaaaatca ggcgaaccct
    10561 acattattca tcctatccaa gtcgcaggta tattagcaga tttaaaaatg gatccccata
    10621 cagtggctac aggcttctta catgatgttg ttgaagatac agaaatcact ttagaagatc
    10681 tgagagaaga atttggcgat gacgttgcta tgttagtgga cggcgtaacc aaattaggga
    10741 aaataaaata taaatcccac gaagagcagc tagcagaaaa ccaccgaaag atgctgctag
    10801 caatggctca agatttacga gttatcatgg tcaaattagc tgatagacta cataacatgc
    10861 gtacgttgaa gcacctgcga gaagataagc aaagaaggat cgctcaggaa actttagaaa
    10921 tctatgcgcc tcttgcacat cgtctaggga tcagccggat caaatgggaa ttagaagata
    10981 cagcacttcg ttatctaaat ccaaaacagt attaccgtat cgtccacttg atgcagacga
    11041 agagagaaga acgtgaaaaa tacgtaagtg gtactgttga agatattcga atagcgacag
    11101 aagagctggg gatttttgca gaaatctatg gacggccaaa acacatttat tcaatctatc
    11161 gtaaaatgaa agatcagaaa aagcagttca acgaaattta tgacttgcta gctattcgag
    11221 tgatcgtaga ttcaatcaag gattgttatg ctgtattagg agcaatccac acaaaatgga
    11281 aaccaatgcc cggcagattt aaggattata ttgccatgcc aaaagctaat atgtaccaat
    11341 ctttgcatac taccgtgatc ggtccggcag gaaatccggt agaaattcaa atccgaacac
    11401 aggaaatgca tgaaatcgct gaattcgggg ttgctgcaca ctgggcctat aaagaaggaa
    11461 aaaatgaaaa agtagaacca gatggtatga cgaaacaatt aagctggttc catgagatac
    11521 tcgaacttca agacgaaagc tatgatgctt ctgaatttat ggaaggcgta aaaggagata
    11581 tctttagtga taaagtctac gtcttcacac caaaaggaga cgttactgag ttaccaaaag
    11641 gatccggacc attggacttt gcatacagta tccatacaga tatcggtaac aaaaccactg
    11701 gtgcaaaagt aaatggcaaa atggtgcagc ttgattacaa attgaaaaac ggagatatca
    11761 ttgagatcat gacttctcca aattcatttg gcccaagtcg cgactggttg aaattagttg
    11821 ctactagcaa agcaagaaat aagatcaaac gtttcttcaa agcccaagat cgagaagaaa
    11881 atgtgatcaa aggccacgaa tccgtggtca aatgtattac agatctagga tttacgccta
    11941 aagatatttt gacgaagaac aaactgcaag aagcactcga tcgttttaat tatcaaacag
    12001 aagatgatct ctatgcagct gtagggtatg gagaagttag ccccttgacg atggccaatc
    12061 gtctgactga aaaagaacgt aaagaacaaa aaatcgagca gcaaaagcaa gaagcagaag
    12121 aaatcatgaa tcagccgaaa aaagaacctg acaaaatgaa agtacgtcat gaaggtggcg
    12181 ttgtcattca aggggtagaa aacttactaa ttcgtatcag tcgctgctgt aatccgattc
    12241 ctggtgatga tatcgttggt tatatcacta aaggcagagg gatatccatt catcgtcgag
    12301 attgtccgaa tgttcagcct gacaaaccaa atgtagcaga acgtttgatt gaagtcgaat
    12361 gggaagatac atcgaataca cgaaaagagt atgatgcaga tttggaaatt tacggctata
    12421 atcgttcagg cttattgaat gatgtacttc aaacagtcaa tgcgctaacg aaaaatctca
    12481 acagcgttga agcacggacg aataaagata aaatggcgac gatccatttg acggttggta
    12541 tccagaattt atcccaccta aagagtatcg tggataaaat caaagcagta cctgatgtct
    12601 acagtgtacg ccggacgaat ggataggagg acagatatga gagcagttgt tcaacgggta
    12661 agtaaagctg ctgtgtcagt cgatcagcaa gaaattggaa aaattggcta cggcctactt
    12721 gttttattag gcattcatga aaccgatacg caaaaagatg tggactatct ggtaaaaaaa
    12781 attgcacaaa tgcgtatttt tgaagatgaa caaggaaaaa tgaatattag tgtagaagat
    12841 gtgggaggac aaattttaag tatctctcaa ttcacactgt ttgcggatac gaaaaaagga
    12901 aatcgaccaa gctttgtgga agccgcacga ccagaaacag ctattccttt atacgaatca
    12961 ttcaatgaag gaatcagaag tcgcggcatc acagtcgcta ccggacaatt cggagcagat
    13021 atgtcggttt ctctcgtcaa tgatggacct gtaacgatca ttattgattc taaaaacaaa
    13081 taaagtaatc tggttttcac tagtaaaaac tatgaaacac gtcgcttaag ttagactgat
    13141 aaactctaac ttaagtggct tttttgtctg caaaaagcgc ttcttttgtc agcagtcgga
    13201 aaaaaagtta agcttaaatt atacaagtta ttttttatag gaggttgctg aagatgaaag
    13261 aagtaccagt agtagatatc aaaatcacag atttaaaaaa attgtatcaa aaaggataca
    13321 acatgttgga ggagttacgc catgaagcgg acgctcccgt cgtcaaagca aagattttca
    13381 ataaagaagc tatcacgatc tacggatcat cagcagcaaa agtattttat gatccaagga
    13441 atttcaaacg aaaaggtgct atgccaaaat tagtattgaa gacacttttt ggtcaaggtg
    13501 gagtacagac tttggatgga gctgctcatc accatcggaa aaatattttt atggatctaa
    13561 tgacaccgga aagaatggaa gattatcatc gtatcttaga taagaatctg acacaagcac
    13621 ttgaagcaca gcatggtcaa tttgaactat ttgatttaag taagatggtt ttctttacat
    13681 ctatttgcga gtgggcaggt atcaatttat cagcgatttc taaagatgag gtcgaaaaat
    13741 tagcagaata tcaaatctct atgatcagcg gaacgtttac ttctccgatt gatcacataa
    13801 aaggtgtcga aaatcgcaaa aaatcagaaa aatgggcgca aggcttgatt gaagaagctc
    13861 gacaaaatcc tgtggctgga aaagaaaatg tggcattata tgcttttgcc aatgccacag
    13921 atttagatgg tcagctttta cctctggaag tggcagctgt tgaattattg aacatcatcc
    13981 gtccaacggt tgctctaact gtgtgggcgg cattgatggg acatgcactt ttcagtagac
    14041 cagatttgta tcagcaatta aaaaatgact tttcgacgtt acaagatccg tttattcaag
    14101 aaatgagacg ctattatcct ttcttcccaa tgctacctgc gatttcgctg aaagaagtag
    14161 aagtagatgg ttatcgtata ccagaaggca gttgggttat tttagattta tatggaacag
    14221 accatgatga acgaacagtt gaagcgccag attcctttat gatcaaacgg tatgtaggga
    14281 aagcaaaaga tatttcctat aaagaagaat atgagatgat tgcacaaggc gggggaaatt
    14341 ttcgacaaat gcatcgctgt gcaggagaat ggatcactct tcatagtcta cgtgtgtttt
    14401 ccgatcagct agtaaataag tttgagttct ctgtacctga acaagattgg acgattccat
    14461 tcaatcagtt tccgacttat ccgaatagcc gagcgctact ttacaagaat tagattagca
    14521 taaacttatc gaaaaaaagg aagatcaaac agtgctgatc ttcctttttt catattacta
    14581 taaattgata aggtcaattg atcaaaatcc acgaaatgct tcatcaatgg tttgataatc
    14641 ttcattggat aaatgcaaat ctaaagcttg gacattgtca gatacttgct ctggacgctt
    14701 agctccaggt ataactacag acactcgtgg attcttcatg taccaagcta aaacaagttg
    14761 cgcaggagtg gcttggtatc ttttagcaat ggggcgaagt acatcgacag cggttaaaat
    14821 ttctctgaaa cgttctcctt ggaaatctgg atttcttttt cgaggatctc cttcaccaaa
    14881 ttgtttttcc tcaccaagtt catattttcc tgtcagcaaa ccggaagcaa gaggaaaata
    14941 gggaacaaaa ctgattttgt ttttttcaag ataagggaat aattcttttt cagcctgccg
    15001 atggatcaaa ctgtatttat cttctactac atcgacatat ccatctgcat tcgcttcttt
    15061 caattgttct aacgtgaagt ttgacacacc gactgctcgg atttttcctg cttcttttaa
    15121 ttcgtgcaat gtagcaactg attcattttt tggtgtgctt tcatccggaa agtggatata
    15181 aaaaatgtcg atgtaatcag tttgcagacg ttttagtgca tcttccaccg attgtttgag
    15241 aaactcagga gaattatcaa atgttcgccc tttatttggt acatgagcag cttttgtagc
    15301 gatgacgaca cgtgagcgat catattcttt ttccttcagt acctcgccaa tcagttcttc
    15361 ggaacggccg tttccgtaag cataagcggt atcgatcaaa ttgatcccgc tattcaaagc
    15421 tgtacgaact acttgttttc ctgtctcatc ttccaaaccg tcaaaaagat tatgtcctcc
    15481 aacggcattt gctcctaatc caagctgttc tgcgtagact tgtgaatgtc cgattcttat
    15541 ttctgccata gctgattcct cctttttgtc tagtctaaaa caaaatacca aaaagctgaa
    15601 ctaaaacgtt tttgtgaggg gaaatagtca gatatctctt gactttgaga ctggttttta
    15661 gtaggttata agtaatatcg aatgtcaatg aaagagcgag taagtttttg aaactttaca
    15721 agagaggatc atcgaggctg aaaatgatct aaagtttcat tagcctaaag acactctgga
    15781 gatggacgag tgaaaagccg tcccgcgtac gagcgttatt cgtaagagga aagagaaatc
    15841 tttcaaaatt aggtggtacc gtgtatgaat ttcattcacc cttgttcatg atgaacaagg
    15901 gtgatttttt tatttacata gctaagaaag ggagagaaaa ctatgagttt ccaaagacca
    15961 aaaggaacaa atgatatttt gccaggcgaa tcagaaaaat ggcagtttgt cgaagaaaca
    16021 gcacgtttat tattcagaga ttatcagtat aatgaaatgc gtacaccgat ttttgaacac
    16081 tatgaagtga tatcccgaag tgtaggggat acaacggata tcgtttcaaa agagatgtat
    16141 gatttttatg acaaagggga acgccatgtg acgcttcgac cagaaggtac agcaccaatc
    16201 gttcgttctt ttgttgagca taaattgttc ggcccagagt atgcgaaacc gttcaaaact
    16261 ttttatatgg gaccaatgtt tcgctacgaa cgccctcaaa aaggacggtt acgtcaattc
    16321 catcaaatcg gagtagaagc atttggatca gaaaaccctg caactgatgt ggagagcatg
    16381 gtgatggcac ttgatttctt caaacagttg gggatcacgc aaatccggct agtcatcaat
    16441 tcattaggtg ataaagaaac gcgtcaagcc tatcgtcaag cattgatcga ctatctttta
    16501 ccttttgaat cagagctgag tgaagactct aaacgccgcc ttcatgaaaa tccattacgc
    16561 gtattggaca gtaaagacaa acgagatcaa aaatttgtgg cagaagctcc atctatctta
    16621 gattatctaa gcgagcctgc tcaaaaacat tttgataccg ttgtttccat gctggatgca
    16681 ttggctgttc cttatgaaat cgacagtaat atggttcgcg gactagatta ttacacacat
    16741 acgatttttg aaatcatgag tgacgcacca aaaatggggg cacaagcaac gatttgcgca
    16801 ggcggacgtt atgacaatct agtggaagaa ctaggtggtc cttcaacacc tggcttcgga
    16861 tttgcaatgg gtatcgagcg attactcttg actatggagg cagaggaagt agttatccct
    16921 gcatttaatg agttggatgc ttacgtggtc gcactcggag acgaaacaaa tatcgaagca
    16981 ttgaaagtgg tccaagcgat tcgaaacttt ggtttttcag ctgaccgcga cttcatgaac
    17041 cgtaaagcaa aagcgcaatt taagacagca gataaagcga atgccaaatt agtattggtg
    17101 atcggtgaag atgagcttgc tagtcaaacg attaatgtca aagcaatggc aagccgaaaa
    17161 gaaaaaagtt ttccattaga agagatatat gaacattttg atgaagtcta tgatgaaatg
    17221 actttattcg agtaggagga aaaaaataaa tggcacaaag aacgatctat tgcggattag
    17281 tttccgaaca acaattagaa caaacagtta cattaaaagg atgggtacaa aaaagacgag
    17341 accttggggg agtaatcttt atcgacctac gtgatcgtga aggaatcgtt caaatcgtct
    17401 ttaatcctga aatcaacaaa gaagcatggg aaatcgcgga taaatgtcgc agtgaatacg
    17461 tgatcgaagt gactggggaa gttagaagac gcggagaaaa tgcagtaaat cctaaaatgg
    17521 ccactggtga attcgaagtg attgcaagtg aaatcgtgat tttgaatcaa gcaaaaaccc
    17581 cgccatttca aattgaagat gatcaagcaa tcagtgacga aatacgtttg aaatatcgtt
    17641 atttggatct gcgtcgtcca aaaatgacta aaaacatcaa attaagaaat gcgacaacaa
    17701 aagcgattcg tcattattta gatgataatg actttctcga tatcgaaaca ccttacctag
    17761 ggaaatctac accagaaggc gcaagagact acctagttcc ttcacgtgtc catgctggac
    17821 atttctatgc gttgccgcaa tctcctcaat tgtttaaaca attgctgatg aatgctggat
    17881 tcgaccgtta ttatcaaatc gttcgttgtt tccgcgatga agatttacgt ggagaccgtc
    17941 agcctgaatt tacgcaagta gatattgaaa caacgttctt aacagcagaa gaaatccaaa
    18001 cttacacaga gggattgatt gcgaaggtaa tgaaagaagt tcgcggtatc gaagtcacat
    18061 tgccatttcc tcgtatgacc tatgatgaag caatggctcg ctacggcagc gacaaaccgg
    18121 atacacgttt tgatatggaa ttgatcgatc taagcgatac agtcaaagaa gtagaattca
    18181 aagtcttcca aatggctctt gaaaacggcg gtgtcgtgaa agcattgaat gctaaaggcg
    18241 ctgcagatcg ctactcacga aaagacatgg atcagttagg tcaatatgta ggacaattcg
    18301 gagctaaggg gcttgcatgg ctgaaagtag aagaagacgg attgaaaggt ccaattgcta
    18361 aattcatggg agaagcaaca gaagcaatca ttaaagcaac agatgcaaaa ccaggtgact
    18421 tgctgatgtt tggcgcagat aaaagtgaaa tcgtcgctgc tgcattaggt gctatccgga
    18481 cacgtttagg aaaagaatta ggactaattg atgaatcgaa attcaatttc ttatgggtaa
    18541 ctgattggcc acaatttgaa ttttcagaag aagaaggtcg atatgtctct gctcaccatc
    18601 cattcacgat gccaaaagaa gaagatatcc cgttattggc tgaagaccct gcaaaagtct
    18661 atgcagaagc ttatgatatc gtcttgaacg gttatgaact aggcggaggt tctatccgta
    18721 ttcatactcg tgagcttcaa gaaaaagtac tagaaactct aggctttaca aaagaacaaa
    18781 tggaagaaca atttggtttc ttgttaacag ctttagatta cggattccct ccacatggcg
    18841 gtattgcatt aggattggac cgttttgcaa tgttattagc cggagaagat aatattcgtg
    18901 aagtcattgc ctttcctaaa aacggaaaag cagcagatgt gatgagtgat gcaccaagta
    18961 tggtttcgcc tttacaattg ttcgaattga atattgatgt aacgaatata gaagaatagc
    19021 cgataaaaag gtaaaaagga tttgtcgaat acgcttcttt tgacaacctt gatttaattg
    19081 aaaaacactt tataatgaag ccataacata taaagcgggg cttttccatg aaaaaaacat
    19141 tcgaaaattg ggcttatcat gattacacga ccaagctttc ggtatctatc gtttatgcgt
    19201 tgatcgcatc agtcgcattg aatttctttt atcagccagg acatatttat tcaagtggta
    19261 ttactggtct agctcagatt ttgacgactt tgtcaaaatc actgatagga ttcgaagtac
    19321 cggtatcaac aactttgtat ttactgaata tacctttgtt tgtattggcg tggttaaaaa
    19381 tcgggaagaa gtttacaatt ttcactttta tcacagtcac tttgacctcc ctttgtatcc
    19441 atattttacc ggaaacagtc ctatctaccg atccaatcat ctgtgcgttg tttggggggg
    19501 ctgtgatggg actaggtatt gggctagcgt tgagaaacgg cctatcatcg ggcggtctag
    19561 atatcgtgag tatcacgatt cgtaaaaaaa caggaaagtc gattggttct atttcgatct
    19621 acttcaatgc attgatcgtt tttgttgccg gaattctatt tggctggcaa tatatgtttt
    19681 acagtacgtt gtctattttc gtcagtggaa aagtaactga tgtcgtttat actaaacaaa
    19741 agaagatgca ggtaatgatc gtaacgaaac atccagaaaa agtagtaaat gaaatccaaa
    19801 actgtatgcg tagagggatc acgatcatca atgaagcaga aggtgcttat aagcatgata
    19861 agcagacagt attgatgacg atcgttacac gatacgaact gcctctactt agagaagcaa
    19921 tgaaagcttc tgatccacaa gcttttgtaa gtattgcaga aaacgtccag atacttggtc
    19981 gtttctatga ggatggcatt tagatcgagt ttgacaaaaa ttcatgaaat actgctgtag
    20041 acagacgttc gtttgtctgc agtttttttc taccatagta gaatcagcaa agaaaaactg
    20101 cttttttatt tgaaattttc ttaaagttga aaaaaagcat gtttattttt ttaaatttat
    20161 gggatgatag aaaaaaatag tagagaaagg acacgattct acttgctatt ttcacttaat
    20221 acccgtatat tacgaatgaa aaaagtttat gaaaagttgg aatattgaat ggaaaaacaa
    20281 aaaaggttaa aaaacagccg ctacatcact gggtttgatg ggctccgttc attagctgtg
    20341 gtgggtgtta tcttctatca ccttctgcca actagtatga aaggcggcta tttgggcgtt
    20401 ccgatttttt tcgtcgtatc tggttatttg atcacggatc tgctgcggca ggaatgggaa
    20461 caaaatggaa aaatcaatat ttggcaattt tatgtcagaa gaatgaagag actctatccg
    20521 gcacttgttt ttatgcttgt gacagcttcg gcttatatca ctttgtttca gcgagggctg
    20581 ttaaacaatc ttcgaggagt tgtggtcagc agcttactgt atgtcaacaa ctggtggcaa
    20641 atcaataacg gtttatctta tttcgatcga tttgctaatg agtcaccgtt tacgcatatc
    20701 tggtctttag cagtagaagg tcaaaactat ttggtttggc cgatcttgtt tgtattgttg
    20761 atggtatttg ttcgtaaaag aaaatggatc ttttatactg tgttaggtac atccttcctc
    20821 tccgccatct tgatgatgat cttgtataca ccaggaggcg atccaacccg tgtttattac
    20881 ggtaccgaca ctcgtctgtt ttctatatgg ttgggaagtg cgcttgcgtt tgtttggcca
    20941 agtacgcgtt taaagaaaaa catcccgaaa caagcgaaaa atattctgaa tatcgctgga
    21001 ggaatctcgc ttttggcact gattctttgc ttcttctttt tagatgatca ttacaatttc
    21061 atctattacg gaggaatgct cctagtgagt ctgttatgcg tagtgctagt tgcagtgaca
    21121 gcacaccccg gagcaagttt gaatcgatgg ctgacaaatc cagtatttac ttggattggc
    21181 aaacgaagct atggcattta tctgtatcag tttccagtta tgatttttta tgaagcaaag
    21241 gtgactgata ttgccgatca tgttttgttg catactttag tggaaatcac attgattcta
    21301 atcatcagtg aactttctta tcgatatgtg gagcgtccgc tagcacgttt tgattatggt
    21361 cgtacattcc aagtgatgaa aggatggttc agcaaaccga tcatcagtaa gagcaaacca
    21421 tgggttgttc caggcacatt acttgtagca attgcactcg ttggtttcgt tacggcaccg
    21481 aaaaacgctg taacagccga tcaaaaacaa ctgcaggcaa aaatcgctga aagtaaaaaa
    21541 ttagcagaag aaaccagaaa caattcagaa aacaccacta gttcggttga ccaagcgatc
    21601 cttgataagt acgagttgtc caaggcacaa gggaataagg cacaaaaatt agaactaacg
    21661 gcatttggtg attcagttat gttggatgct gcaacggatc tacaagaact ttatccaaaa
    21721 gtagtggtag atggagatgt cggacgacaa ttatatgcca gtgtaccgta cattgaaaaa
    21781 ttaaaggaac aagatttgtt gaaagacact gttctgatcg gactaggaac gaatggctca
    21841 ttctcggaag ctcagtttga tagtgtgatg gatgcaatcg gtgatcgaaa agtatactgg
    21901 gtcaatgtcc gagtaccgac gaagcgctgg caaaatgacg tgaatgcgat gcttgaacag
    21961 atgacgaaaa aatacgacaa tatgacgctg attgattggt acagctacag taatgaccat
    22021 agtgactggt tttatgatga tcaagtccat ccaaatcctg aagggatgac acattacatt
    22081 cacttagtca gcgaagcctt gctaggagga agttcaagta cgaacagcag tgagacaaac
    22141 gtgacagaat gatagctaca aaaaacttca ggcaaaaaca tttgtttttg cctgaagttt
    22201 ttttatattt accgtgtttt taacattcat atagattcag gaagcaaaac ggttgagtgt
    22261 gtctgtaaat aacgttcttt ttgttttcgg gtgagttttt tgaatgcagc ttctgctttt
    22321 gttgcttcgc ttcttgaaga aaatacttct gcatggatca tcttaacagg tcttcttttt
    22381 tgaagacgag tatatttagc accagtacca ttgttatgtt cctctaatcg tctagaaaga
    22441 tcggttgtat atccaccata aaatgtctga tctttacaga ataatacgta aaaataatgt
    22501 tcatttgcca tagagcatct cacgaatctc tgcagtgtat tctccatcca tattgtaagt
    22561 gatcaatggt ggagcgactt tgaatccgtc taatttgcct tctttgattc cttcaactaa
    22621 caaaatattt gcctcttttc caggttttgg gtaaacaaat tggatccttt taggtgcaat
    22681 tcgatagtct tccatgacct gaatgatttc taagaaccga tcaggacgat ggaccatagt
    22741 aaagcgtcca tttgttttta gtagcttact tgaaatatcg atcacttctt ctaacgtcgt
    22801 atgaatctca tgacgggcga ttgccaggtg tttattaggg ttcgttttat ttgttggcag
    22861 ccctttgaaa taaggtggat tacagacgac taagtcacaa gaattatgtc gaacgacaga
    22921 taagctgtct ttcagatcga tggtgtggac agtcatttgt tcatttaaat ggttaagcgc
    22981 gatactacgt cttgccatgt ctgctagacg aggttgaagt tcgatagcgt cgatagccgc
    23041 ctgggttcgt ttacttaaaa aaagtccaac tgcgccattt cctgcacaaa ggtcaacaat
    23101 cttccctttt ttaggtacac gggaaaagtg agcaagcagc acagcatcta aagaaaaaga
    23161 aaacactttg gaactttgga taatcttgat atcttctgcg tataattggt cgattcgttc
    23221 gtcttcgtga agcatggatt ccctccaaaa tgaaaaaaat agtcgtctgt attatactat
    23281 ttttattaaa aagaaccaag gtggcttgtc aaacctctat tttttatgca tactatgacc
    23341 ataagaaggg aaaggaagag actatgtttt atcgatttat gcgcggggta gttcgagtac
    23401 tattagcctt agttaatgga aatgcccgct atgagaataa agaagtttta ccgcaagatg
    23461 agaattacat actagttgct ccccatcgta cttggtggga cccattatat ctggcggtag
    23521 ctgcagcacc caaggaattc agctttatgg caaaagaaga actgttcaaa aatccagttc
    23581 tgcgatttat attaactcga tccaatgctt ttccagttaa aagagaaaat ccaggaccaa
    23641 gcgtcattaa aacacctgta aagattttga aatcgacaaa tttgagtttg atcatgtttc
    23701 ccagtgggac aagacatgct acagaactta aaggcggaat ggcattgatt tccagaatgt
    23761 ctaaagcgaa aatcgtgcca gctgtatatc aaggtccgtt gacacttggg gatctgttca
    23821 agcgtcagag agtgacagtg cgtttcggcg aaccaatcga cttatcagat atcaagaaaa
    23881 cggataaaga aggtatggcg gagatcgaac gacgaacaca agcagctttt gacaaattgg
    23941 acaaagaagt gaaccctgat ttcaaatatg aagtcaaaaa aaaataaaat gataagttta
    24001 tgcgtgggcg gtcttttgaa tctactgtcc tgttcagttc agactttttc aatgaaaacg
    24061 agggagagac gtcaagtctg ccctcttttt gcgttccaat atgtaaaata gaagacaggt
    24121 gtcatgtaat ggatcaaagt gttataataa ttagaaggac attttttgag gtgattgaat
    24181 gcgtttttta catacagccg attggcatat cggaaaaaaa ctacaaggtt atgacttgat
    24241 tgaagaccag cgtcatgtac tatcttctat actagatatc gcgaaagaag aaaaagtaga
    24301 ggcaatcgtg attgccggag acttatatga taggagtgtc ccagctgttg atgccgtaga
    24361 attatacaat gaattgatgg tcgattggaa tttaaaggaa aagatcccga tttttgctat
    24421 atcaggaaat cacgatagca gtacgagact ttcagcgggt acgccatggt tcagttattc
    24481 gaactatttt ttgcgaacac agctagcaga agcatttgaa ccagtcgagt ttaaaaacgt
    24541 acaatttttc tttcttcctt actttgaacc aattgcagcg agattatatt ttgaagatga
    24601 caatatccga acgatcaagc aagcggtagc caaggtgata gataaaatgc aggagctatt
    24661 tgattcagaa aaaaaacaag tattgattag ccattttttt gtaatgggaa gtgtaaaatc
    24721 tgattcggaa acgaaaatcg aagtaggcgg gttggattcg atccctggtg aactacttac
    24781 accgtttgat tatgtagcac taggacattt gcataatcaa gctgctttga gacaggataa
    24841 tgctcgctat agcggttccc cattgaaatt ctctttatct gagatcaacc aaaaaaaagg
    24901 cgtatggatc gttgacctga caacaggcct ggagttgaat tttagggaga tcgaacccct
    24961 gcatgatatt gaagaaataa cagcgagttt caaagaattg ctggatcctg ccttttatca
    25021 gtccgtcaat cgggagaatt acatctatat ctatctcact gatagaacga tcatcccgaa
    25081 tatgatgaac cagcttcggc aaatatatcc tcgtattttg gggattgaac gggcaaacgg
    25141 tcgagcagaa caaacaaagc gcaaacgaaa agtactagaa atgaaagatc ctcaaaaatt
    25201 agcagaagca ttttttagtg aaatgacgga agagcagcta actgcaaacc aagaaaaatg
    25261 gctcggagaa acgttgcaag aaatcatgga aatggaatga tcaagaatga aaccgaaaaa
    25321 attacaactt aaaaattttg gtccttttat caatgagacc gtagattttt cacgtttgac
    25381 agaagcaccg ctttttttga tcagcgggaa gacaggagct ggcaaaacga cgatttttga
    25441 tgggatcacc ttcgcacttt ttggtgaaac ttccggaaga cttcgctctg gaaaagagat
    25501 gcgttctttg tttgctacac cagacgaaga aacaagcgtg actttttctt ttgaacatca
    25561 gcagatgagc tatgaaatcg aacgaaaacc agaacaagtt ttagcaaaac gaaaaggaaa
    25621 cggaacaaaa aaacaggcag ccaaagtaac gttgaccatt tttgatggga aaggaaaaga
    25681 aataaaacaa atcacaaaac gtacagaagc agatcagttg atcagagaac tgatgaatct
    25741 cgatgcgaaa cagttttctc aaatcgtttt actaccccaa ggagaattcc gtaatttttt
    25801 ggtttcatca agcagtgata aagagactgt tttacgtcat ttattcggta ctcaattttt
    25861 ccaacagttc aatgagcgat taaaagaaaa agcaaaacaa cagcaacaaa aattggatca
    25921 tttagagcaa gagctgcatc ttctgcaaaa aaggtttgtt ttagtcaaag aagaggaacc
    25981 agcagcaggg tttgaagccg ttttagccca atggacaaac catcaggcaa tactcgaaaa
    26041 acaaatcacg gaagaaaaag aagctctaac ggaacttcag gaaaaacaaa agcaactaga
    26101 aaattcctac tatcgttttg aaagcttgaa aaaaagttat gaagaaaaaa cgatattaat
    26161 aaacaagcaa aaggaattag ctgctcaaaa agacgaaata gaggaaaaga aacgatggat
    26221 tgggtattat gaatttacgc aacgattagt cgaaccaatc cagcgcctac aagaaataaa
    26281 agttgaaaaa atagaactgt cacaaaaaag tagtgagcaa acaaagaaac tagcgatgac
    26341 tacgcagaat tatgaagagt ggcaaaaaaa tgaagcaaac cgcgtacgcc gacaaacaaa
    26401 aatcaaagaa gctgctcaaa aactgcaaaa caaacagatg atggtgccaa ttgtcgaatc
    26461 cttacgtagt aaaaaacaag acgcagaaca attaacaatt tcattggata aagaagaatt
    26521 gaatgccgag aagctgtatc aaaatctcgc tatctatcag gaaagaactc gaattcttca
    26581 agaaaattta ctggatcagg aaaaactgca agaagatcgt cttgcttttg gacagttaaa
    26641 atatctagat gaaaagcagc aagaaataga aaaacaaaaa gcaaaagaca ctgggtcgct
    26701 cgcacagata agagaagaac tcaaaaaagc aactgaagaa ataacaaaaa atagtcagct
    26761 attgaaagaa cagacagaac aagcagaaca gctgaaaagc caatgggcaa agcagcaaat
    26821 tgctcgtctt cagctttttt tgcgtccagg ggagccatgt cttgtttgtg gatcaacgga
    26881 acaccccata caacaattaa aacacgaaca gccaaataag gaagatatct taaaagtaga
    26941 acaacaactg gcagaagctg agtctgctgt agaaagaact cagcaaatca ttgccaaaag
    27001 tgaagcacat gaaaataatc ttcaacaaca attatcagat ttagaacaaa aaatcatggc
    27061 aacgaatata caattggctg aacaagaaga acacttgcaa caggctttga aaaaggcatt
    27121 tcctgagata acagaacaaa aaacaaaaac aactcttgta gtaatcgaac aatctttaga
    27181 tagacgaaaa agcaagatgg ccaatgcaag aatagagctg gaagaaataa gacaacaagc
    27241 agaaaagctc gaacaacaat tagctgtcta caaacagcag cagacagaaa aaaataatca
    27301 gctccagcaa ctgcaaggag aaatcaaaag tttagaacaa caattgcaag atacggatag
    27361 taactgttta gaagaggaga tacgacgact tgaaaaacag ttagaagaag actcgaatca
    27421 actagctgtt gagcagaaag caggagtgca attgttgcaa gatcttcata cgcttcggac
    27481 acagcttgat cttcaacatg aacaaataga aaaaatagat gaaaaaatag aaaaggaaca
    27541 agaaagaata gctaaaaagt tagaaagcca gtcgtatttt tcaacaattg aagaaatctg
    27601 tgcatttgca caacagcaag cacaatataa agaaaaccaa cgtacagtta cttcttatca
    27661 tgaagagcaa ttagtgacag ctgaccggct gcgtcaatta gaaaatatcc agctaactga
    27721 gacattccca gatacagaag agatcagcca acagttagaa gaaataagac gtatgatcga
    27781 aataaagcaa gaaaagatat acggtttaca agaacaaaaa attagtaatc aaaaaatcta
    27841 cgatgaatgt ctttcgattt atcacacaag ccagacgaaa ttagatgagc ttacgcaatt
    27901 acagcagctt tcgcaaacat tcaacggaga aaatcctaag aagactagtt tggaacggta
    27961 tgtgcttcag gtttatcttc aagaagtgtt acaagtagca aatgatcatc tccaacgttt
    28021 aacgaaaaat cgttatcaat ttgagctagc ggataccatt ggcagttatc gagggaaaac
    28081 cggattagaa atcaatatct atgatgatca ggcaggtatg tctcgaagtg cgcataccct
    28141 gtctggcggt gagagtttta ttgcagcact ttctttagca ttagctcttg ccgatgtgat
    28201 ccagacacaa gctggtggtg tagcaatcga tgcattgttt attgatgaag gatttgggtc
    28261 attagatgaa gaagctttgg aaatggcaat ggaagcttta gaaacgatcg aaagtgaagg
    28321 aagaatgatc ggcatcatta gtcatgtaag agaattgaaa gaacgagtat tacaacaaat
    28381 aagaattgat acagaaggta gcggacaaag caaaatacgc tatcaacttg gataaaaagg
    28441 gagattggta actatgaaat ggagtattcc agaaaaagtc attgcacgtg gacgtcaata
    28501 cttgcaagaa ggccgagtgt tatcggttgt ccctgaccaa gaagaaaacg tgtggcatgc
    28561 agaagtcttg ggcagtgaat tgtatatggt tgatttggat ggtacagcaa aagagataga
    28621 ttactgccaa tgtccctatt gggaggaaca taaatattgt aagcatacag ttgctgtgga
    28681 actttattta cggtcgaaaa atctatctcg tatcatgaaa aaagaccagc caatgctaga
    28741 accggtaaaa gcaacaagtg agggagaaat cattgcaaaa ggtttttctc gtctaaaaac
    28801 acaaaacaat cctcaagcgg tgcaaccttt gattattgaa taccaagtag aaacgattga
    28861 gactaatcag tatcacccag agctttctat tttggcactt tacttgcgta tcggttcatt
    28921 ggcatcaccg aaaaaaacat atatcgtaaa aaacatctat gattttttac aagcttacac
    28981 agaaaaagaa acttacactg ttaataaaca gtaccagttt cgtctagata aacgggcgtt
    29041 tcaaaaagaa gatcaaaaaa tactcacgca tcttgcagga agtgctcaga cgcatcaatt
    29101 attaggtacg aacggcttac aagtcaaagg aaaactagat aaaagatatc tgcttctccc
    29161 agtagaacaa gcccgcttcc tgttagagca gatgaatcag actacacgtt tgcatctgca
    29221 gataaatgaa caaaacgatc aaggaataca attttccgat caaaacaaac ctttatcatt
    29281 caaagtggaa aaatttggag aagattattt gctgcaagca ttgaatgatt ttgatttttt
    29341 ctttatgcat tatcagtggg gagttatcca agggacattt tattctctga cgaaatatca
    29401 acaaacggtt tatcaaactt ggaaacaatt gattcgccga ttggagaaac cagaagtagt
    29461 gttcaagaaa aaagaattac cgtctttgtt taaagagatt atccctctgc tgtcagagat
    29521 cggcgacgta gctattgccc ctgcagtaga agaggaagta gcagatatcc ccgtcgaatt
    29581 tattttcttt ttccgaaaag caaaaggaaa aatccaagct cgagtcgatt tcgtctatgg
    29641 cgaagtcatc tattcgactg acccgaagca tgaggtctcg acagaacagg cttttcaggt
    29701 gttacgtgac acagtagaag agaaacgagt acttgatcta ttcagaatgt atcgctacca
    29761 gaaaaatgaa actggttatg aacgtccatt gccaagcggg gaagaactat atgccttttt
    29821 ccgcacagaa ctatctgtct tccgtcagtt tggtgaagtc agacttggaa gaaaattgcg
    29881 agagttgtat ttagatgcgc ataaacatca accgaaaatc gaagtaaccg agaccggttc
    29941 atggctcgat attcgattcg atgtgacagg gatagaggag caagaaatcg atcatgtcct
    30001 gcaaagcttg ctgagaaatg atgcttttta tacattagaa aatggtcagg ttctttcttt
    30061 tgattcagaa gaatttcaac aaacaagcca agtgttgcaa caattacggg aatcgatacg
    30121 tacagaagaa ggaacgatcc atgtacctaa aaatcaagga ttgatcattc aaaatcaatt
    30181 ggaaaaaagc aatgctactt ttagtgaatc gttccaaacg atggtacaag atctcattca
    30241 tccagaaaga taccaggcac agcttcccaa aggattgaat gcaacgatga gagattatca
    30301 aaagcaaggt tttcgatggt tgaagatgtt aggtcattat cagtttggcg gtattttagc
    30361 cgatgaaatg ggacttggga aaacactaca gacaattgcc tttttacttt cggaaaaaga
    30421 agaacggaaa agcttctctg ctttgatcgt agcgcctgcg agcttaattt acaactggca
    30481 ggcagaggtg agaaaattcg ccccttcttt aagtatccaa gtaatcaatg ggaataaaaa
    30541 agaacgtgaa gaattactgg ctaaagacac agatatccga gtgacttctt atgccagctt
    30601 aagacaggat ctagctaatt atcagtcgca aaaaattgat tatttgattc ttgatgaggc
    30661 acaaatggtc aaaaatagca gtacgaaaac tgcacaagca ttgagagaat tagctgtacc
    30721 tcagcggttt gcactgagtg ggacaccaat cgagaataat ttagaagaat tgtggtcatt
    30781 gtttgcaacg attatgccag gatttttccc gactaaaaca aaattcaggg aacttgccgc
    30841 agaagaaatt gcgcaaatga ttcgaccgtt tattttacgg cgagataaac aaactgtgtt
    30901 gaaagacttg ccggaaaaaa cagagatgaa tctatattct gcacttacag aagaacaaaa
    30961 aactgtttat ttagcttact tgcgtcaaat gcgtgaagaa atcacatcaa tggattctga
    31021 agcattcaag aaaaaccgga tcgggatact tgctggtctg acaagactgc gacaaatttg
    31081 ctgcgatcct cgtctgttta ttgaagatta ccaaggcggt tccggcaagc tcgaacaagt
    31141 aaaagatcta ctggtagctg ccaaagagaa caaacgaaga gttttgctgt tttctcaatt
    31201 tacagggatg ctgactatct tgcaagaaga gttagccgag ctagggatct cgacatttta
    31261 tttaagaggc agtacgaaac cacaagatcg gttaagtatg gtagacgcat ttaatgctgg
    31321 cgaaaaagat gtttttctga tttccttaaa ggctggggga actggattga atctcacggg
    31381 agcagatacg gtcatcttgt atgacctttg gtggaatcct gccattgaag aacaagctgc
    31441 tggacgtgca catcggatcg gacaaaaaaa tgtggtagaa gtgtggcgta tgatctctga
    31501 aggaacgatt gaggaacgca tggattcact ccagcaagaa aaacgcgaac tgttccaaaa
    31561 agtgatccaa ggtaatgaag aacagttgac gaaactgact gaagaagaca tccgcatgat
    31621 tttaagtgta ggagaaatcg aggagtaaga gactcgaaaa agcgttcagt aatgggtaag
    31681 aaagcctttc taaagaagcg attgtttatt tgatttaaaa aatagcaagg aggctgggat
    31741 tccattttat cccagcttcc ttgctatcat ttttttattt ctatttttaa aggttataca
    31801 aagattgtct tcattgtctc taacagctga tcaaaagaag aaatctcgat atcgcaaatt
    31861 ggagtctgtt caattttgcg ttctcgatgg ttgaaccaaa gcgcttgcca gttagcactt
    31921 tttgcaccta gaacatcatt gtcgtaatta tcgccaacgt ataaggtctc ttctggaagc
    31981 atatgaaatt ctttctcagc tagctgaaaa atctcttttt ctggtttttg gaaacctgtt
    32041 gcttgagaaa tgagcatatt gcgggaagga atccaattgt tcaattgaag ttgcttcagt
    32101 ttttttgtct gatggtcagt cggcccattt gtgataattc ctaatgggac attcttttcc
    32161 ttcaagaaat cgagggtttt tttgacctcc tcatgcaagc agatattatc aagttcttcc
    32221 tcgtaaattt tttgaaataa gaggccatct tcttccgtaa tatgagggta gtctaaatct
    32281 tttagtgatt ggctgatccg atgtgctcgc atatattcta gtgtccaatc tcctgccatt
    32341 acttttggga agttttcatc actatgatgg cggaaccgga tatatagtgg aagcatatct
    32401 gcatctgaaa caagcggaac gactcttttg accgcgtttc gaaatggctg ctgctgatca
    32461 tacatcgtat catcaacatc aaacacaact gatttaatca aaattttaca cgcctttcgg
    32521 ttttcattga ttttttcaca aatccttgac tattgtaaca gaaaaaattg tctaaaaaag
    32581 ctgataaata aaggtttttc ttactatatt tctatttcct tgacagaata aggtttttat
    32641 atttcagttt ttttcactaa aaatcgtgaa acacgtcaat aagtttgatg aacaaattaa
    32701 tcattatggt agaagtcgat tgatttattt taatgcaatc tctgtggaat tttcgaaag
//
LOCUS       DAZABD010000030        32753 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000030 DAZABD010000000
VERSION     DAZABD010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32753
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.030"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..142)
                     /locus_tag="ACHALG_001432"
     CDS             complement(<1..142)
                     /locus_tag="ACHALG_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFN2586850.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKW
                     VKQ"
     gene            351..725
                     /locus_tag="ACHALG_001433"
     CDS             351..725
                     /locus_tag="ACHALG_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFN2586851.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(839..1468)
                     /locus_tag="ACHALG_001434"
     CDS             complement(839..1468)
                     /locus_tag="ACHALG_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFN2586852.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1602..1674)
                     /locus_tag="ACHALG_001435"
     tRNA            complement(1602..1674)
                     /locus_tag="ACHALG_001435"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1639..1641),aa:Thr,seq:ggt)
     gene            1888..1961
                     /locus_tag="ACHALG_001436"
     tRNA            1888..1961
                     /locus_tag="ACHALG_001436"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1922..1924,aa:Asn,seq:gtt)
     gene            complement(2099..2263)
                     /locus_tag="ACHALG_001437"
     CDS             complement(2099..2263)
                     /locus_tag="ACHALG_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2586853.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            2558..3886
                     /locus_tag="ACHALG_001438"
     CDS             2558..3886
                     /locus_tag="ACHALG_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HFN2586854.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            4088..4921
                     /locus_tag="ACHALG_001439"
     CDS             4088..4921
                     /locus_tag="ACHALG_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFN2586855.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            4937..5674
                     /locus_tag="ACHALG_001440"
     CDS             4937..5674
                     /locus_tag="ACHALG_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HFN2586856.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(5829..6797)
                     /locus_tag="ACHALG_001441"
     CDS             complement(5829..6797)
                     /locus_tag="ACHALG_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFN2586857.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            7029..7862
                     /locus_tag="ACHALG_001442"
     CDS             7029..7862
                     /locus_tag="ACHALG_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFN2586858.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            7896..8735
                     /locus_tag="ACHALG_001443"
     CDS             7896..8735
                     /locus_tag="ACHALG_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFN2586859.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            8761..9321
                     /locus_tag="ACHALG_001444"
     CDS             8761..9321
                     /locus_tag="ACHALG_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFN2586860.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            9520..11241
                     /locus_tag="ACHALG_001445"
     CDS             9520..11241
                     /locus_tag="ACHALG_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HFN2586861.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIEKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQRYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVDMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            11406..12548
                     /locus_tag="ACHALG_001446"
     CDS             11406..12548
                     /locus_tag="ACHALG_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFN2586862.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            12564..13775
                     /gene="thiI"
                     /locus_tag="ACHALG_001447"
     CDS             12564..13775
                     /gene="thiI"
                     /locus_tag="ACHALG_001447"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFN2586863.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            14348..14995
                     /locus_tag="ACHALG_001448"
     CDS             14348..14995
                     /locus_tag="ACHALG_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFN2586864.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    15124..15340
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            15388..18033
                     /locus_tag="ACHALG_001449"
     CDS             15388..18033
                     /locus_tag="ACHALG_001449"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFN2586865.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            18343..19656
                     /locus_tag="ACHALG_001450"
     CDS             18343..19656
                     /locus_tag="ACHALG_001450"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HFN2586866.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            19646..20299
                     /locus_tag="ACHALG_001451"
     CDS             19646..20299
                     /locus_tag="ACHALG_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFN2586867.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            20354..21046
                     /gene="radC"
                     /locus_tag="ACHALG_001452"
     CDS             20354..21046
                     /gene="radC"
                     /locus_tag="ACHALG_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFN2586868.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            21196..21396
                     /locus_tag="ACHALG_001453"
     CDS             21196..21396
                     /locus_tag="ACHALG_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFN2586869.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(21650..23329)
                     /locus_tag="ACHALG_001454"
     CDS             complement(21650..23329)
                     /locus_tag="ACHALG_001454"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFN2586870.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(23331..23543)
                     /locus_tag="ACHALG_001455"
     CDS             complement(23331..23543)
                     /locus_tag="ACHALG_001455"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit epsilon"
                     /protein_id="HFN2586871.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            23936..25666
                     /gene="efrA"
                     /locus_tag="ACHALG_001456"
     CDS             23936..25666
                     /gene="efrA"
                     /locus_tag="ACHALG_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HFN2586872.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            25663..27423
                     /gene="efrB"
                     /locus_tag="ACHALG_001457"
     CDS             25663..27423
                     /gene="efrB"
                     /locus_tag="ACHALG_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HFN2586873.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            27518..27715
                     /locus_tag="ACHALG_001458"
     CDS             27518..27715
                     /locus_tag="ACHALG_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2586874.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            27868..28353
                     /locus_tag="ACHALG_001459"
     CDS             27868..28353
                     /locus_tag="ACHALG_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFN2586875.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(28457..29050)
                     /gene="clpP"
                     /locus_tag="ACHALG_001460"
     CDS             complement(28457..29050)
                     /gene="clpP"
                     /locus_tag="ACHALG_001460"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFN2586876.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            29230..29757
                     /locus_tag="ACHALG_001461"
     CDS             29230..29757
                     /locus_tag="ACHALG_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2586877.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            29848..30585
                     /locus_tag="ACHALG_001462"
     CDS             29848..30585
                     /locus_tag="ACHALG_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFN2586878.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            30589..31506
                     /gene="miaA"
                     /locus_tag="ACHALG_001463"
     CDS             30589..31506
                     /gene="miaA"
                     /locus_tag="ACHALG_001463"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFN2586879.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            31508..32737
                     /gene="hflX"
                     /locus_tag="ACHALG_001464"
     CDS             31508..32737
                     /gene="hflX"
                     /locus_tag="ACHALG_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFN2586880.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT        10827 a         5563 c         6910 g         9453 t
ORIGIN      
        1 attgctttac ccatttgtca aatgctgaag ccgttagttc atattctcga atgatttcta
       61 cacgtggctt tccagctaag taaagattga cgatttgttg cttgaattct tgtgaaaaag
      121 ttcttcgtgt tctcttagac ataaaaattc ctcctggtat gttttcttct agtctacaca
      181 ccttaatttt tctgtctagt taattgtagc ctatccaagt ctacactctg agatgtaaga
      241 aatatcataa gatatttctt acatcttttt gttttgagca ggactttaga taattttatt
      301 aatatatgct agcatgcttt tgtaagaaaa agaaagaggg cgtattagtt atgtatctta
      361 aaattacgga acaagcacaa gaaagattac aaaaatatat tgatgaaggc gcaacagtta
      421 tattggattt agatgatggc gttggggaat attctaaaat gggtgtttgc tctttagata
      481 ctagttttcg tttacttctt cttgataaag aacagagaaa aaatgattat aaactccaat
      541 tagatagtga tattggggat gtttatataa aagattattc aaaaatgtac atggatgaac
      601 atatgaccct ttctttagat ccacgtctag gtgtatttac tcttgaaagc ccaagtggtt
      661 ctttagacag tcatgttcaa ttagtagatc tacgaccaga aaaattctct ggaataaaac
      721 aataactttc tctctattgt ccataaaact agtctcaatc aagctgtatt gaaaaaacaa
      781 gacaacattc ttaccatggt aaataaaaag acgacacatt ttgtcgtctt tttttaaatt
      841 aactattttc gataaaagat cgcatagctt gtgccattat ttctggcttt tctctattca
      901 attcatgttt tccgttttta ataaagagta gccggccatt cgttgaaagt ttatataaac
      961 gttttgctgc tggtttattc gcatgatctt tggtaccgca taaaatcaat ttaggaagtc
     1021 gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag tgaacgaagt agggtaaggg
     1081 tttgtttttt agatagacct aatttttcaa atatcttaga aggtacgcag taaaaataaa
     1141 tattctggaa agtaagcaat aagcgattag gagactcaaa ttgtggagcg actaaaacaa
     1201 ccttttttaa atatggagaa ggagttgcaa gatagtttaa agcaataaca gcgcctaaag
     1261 ataatccaca taaaataaaa ggttccttta tctgattcaa ttgtacttcg atgtatttgg
     1321 ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg attgaacaat gaaagacttt
     1381 tgcaagaata actattgggt agataagaga ttacatcttt ccatgattgt tcgtcttggc
     1441 ctagaccatg caaaaaaaca attttcatat tgctctcctt tttatagtta tcatatagac
     1501 tttctttgta ctattaaaat ataaacattc atttcttacg tcataaaata aaaaaacagc
     1561 gcagacacat tgttaaacca acatgttgca ctgtttaagg aagccacttg ccgggcttga
     1621 accggcgacc tcttccttac catggaagcg ctctaccagc tgagctaaag cggcattatc
     1681 attaatacaa ataataggat aacgaatatt aatgtatttg tcaacagaat tttttcgaaa
     1741 aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa taaaaataac aaaaaatgcg
     1801 aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa gtattgcgtt atttagaaga
     1861 aattgatatt atattcatgt acttgattcc ggcatagctc agttggtagt agcgcatgac
     1921 tgttaatcat gatgtcgtag gttcgagtcc tactgccgga gtaaataaag cttatgcaaa
     1981 gaaaaagaaa tccaatcact tgggtttctt tttcttttat tattatgatg aatcaagctt
     2041 tctgtttttg tcacgtatgt tgtaatcttt acgaaagagt agagttggct actcataatc
     2101 aaccatactt cccatcatcg ctaaatacga aataaatcag cattcctaca ccaatcaaag
     2161 cacaagctgc accaagaaaa gggatgacat tcccaaagtt atctgtattc ataagtatgc
     2221 caccacccac aaacaaaaag ccgccagcaa tcaggttttt cattttattc atctcctttc
     2281 ttacgttata gtatttcctt ctttcaatga gtattttgaa agctatctta ttgtatgatg
     2341 ttcttctagg cctcttcttt attatacagg ccattttcgt tatctcatag aaatattcct
     2401 ttttatctga aaactattgt ataccgtatg acatttttct tcatgaagtt gtaagtatat
     2461 tctcattcaa atgaaaattt catcatagaa gagattgtat ttctaaattc ttaagagtaa
     2521 aatacgaagt ataacttgga tttggaagga tgattggatg aaagctatta cgcctgatat
     2581 gacaacaaaa tttaacaaac agtttcaagc aaatatcaaa cagcaggcat tgcagcgggc
     2641 agttgtgaag aacggaatta ctgcttctgc tgaaaatgtt tcagcacatg tggagaatac
     2701 accggtattt tctatagatt tggctacggg gaaagtggct aatcaaatgc aatcggggcg
     2761 ttgctggatg tttgctgcac tgaacacatt ccgtcataag atgctgaatc aattccattt
     2821 aaaagagttt gaattatcac aaaattacac ttttttctgg gataaatatg aaaaggcaaa
     2881 ttatttctat gaaaacatca ttgcaacagg aaaagaggca atcgatagcc ggaaagtagc
     2941 tttcttactc gctactcctc aacaagatgg cggccaatgg gatatgatcg tttcattatt
     3001 ccaaaaatat ggcgttgtgc ctaaatcggt gatgccggaa agtgcgaata gttcaaattc
     3061 tcgagatttg aataactact tgaacaaatt actgcgtaaa gatgcagtag tgttacggaa
     3121 aatggttgct caaggagaaa cattagacgc tattgaagaa aagaaagaag aaatgctaga
     3181 aagcatttat aacttcttag ctatctcatt aggaacacct ccaaaagaat tcgattttga
     3241 gtaccgcgat gaagaaaaga actatcatct tgaccgagga ctgacaccac aaatttttta
     3301 tgataaatac atcggtgtaa aattagatga ttatgttagc gtgatcaatg caccaacaaa
     3361 agacaaacca ttcaatcgtt cttatacagt agagatgcta ggaaatgtcg ttggcgggaa
     3421 agaagtcaaa tatctgaatg ttgatatgga aactttcaaa aaattagcga ttgctcagct
     3481 tgaagaagga gaatctgttt ggtttggttg tgatgtaggt caatcttcta ctagaactag
     3541 tggcatcatg gccttagatg cttattcaat ggatgatcta tttgatacgg attttacaat
     3601 gactaaagct gaacgtttag attttggaga aagtctgatg acccatgcga tggtattgac
     3661 cggtgttgac ttagtagacg ggcaatcaac aaaatggaaa gtcgaaaata gctggggcga
     3721 aaaagttgga aagaatggct tctttgttat gagtgatgcc tggatggacg aatatacgta
     3781 ccaaatcgtt gtaagaaaag atttattgac gaaagagcaa ttaaatgcgt tcaatgaaga
     3841 accaattgtt ctatctccgt gggatccaat gggtgcttta gcatagacga gtatttcaat
     3901 gaagagtatg tagtaaagat aaaaagagct ggcttaagcc agctcttttt atctttaaag
     3961 tccattccct gttttttatg gtccctgtgc tataattcta aataaaggat aaggattgga
     4021 aataatcctt ttatcattaa aaatatgatg acgaagtaga ataaaattgg gagtaggggg
     4081 gaaagtcatg gatatccaaa ctgttttatc tgatctgaaa ttaaatggta aagtgattcc
     4141 agttgttggt ggcgatgtta atcagaccta tcgtataaaa accgaacatc gtgcttattt
     4201 tttaaaaatc catccgaatg taaaaaaagg attttttgaa gcagaagtag acggattaaa
     4261 agaactgtca gcattcgtac gtgtaccgga tacgtatatg ctaggtgaaa caagtgaagg
     4321 cgcttatttg ttaatggaat ggatcgagcc gggaaaaggg gatcaacgag atcttgctgc
     4381 agctttagct aatctccatc agcaaacagc gccacaattt ggttttcgta aagataacta
     4441 tttgggtaca cttgttcaga aaaattcttt tgaagaagat tggtggactt ttttctttaa
     4501 agatcggctt gaatcccaga tttctttggc agaagaaacg aatcgatgga atgtacagcg
     4561 tcaagaaaag tatctgcgat ttaaagaacg tgtattgaaa tcggttgaac caaaaaagat
     4621 cactccacgc ctcttgcacg gagacctgtg gagtggtaat gtgttctttg accagcaagg
     4681 gcatccagtt tttgtcgacc cagctgtttc ttatgggaat cgggaacaag atattgcaat
     4741 gagccagtta ttcggtggtt tccgaccgga atttttagat gcttatcaaa ctatatttcc
     4801 attagaaaag ggatggaaag accgactgcc gatttaccag ttatattatc ttttggcaca
     4861 tttgaatatg tttggagaaa gttacggttc ccaagtagat caattactag aaaattttta
     4921 agaggagggc gtgtttttgg caaaaaaata taccaaaaag catgaagttt catattatga
     4981 gtgtgacatc aaccaaacga tgacatttcc ttcgatgtta ggagtagtta tcaaaacgtc
     5041 tgaagatcaa agcgaccagt tgggaagagg cagtgaattc gtcaatagct ttggacttac
     5101 ttgggtcatc acgaattatt ccatggagat taatcgtctc cctagagtcg gtgaagtaat
     5161 ctcagtaacg actcaagcca tggagtacaa taagtttttc tgttatcgga acttctggat
     5221 ccatgatgaa gatgggaaag aattagtcaa aatagaatct gttttcgtat tgatggattt
     5281 tgtgaatcga aaaatgagca gtgttaatga agagatcatt gctccttttg agagtgagaa
     5341 gatcaagaaa atcaagcgtc aggaaaaaat tgaaaagatt gattcgggct tcatgttgcc
     5401 atatcgtgtt cgtttttatg atatcgatag taatcagcat gttaataatg cgatgtattt
     5461 taattggatc attgacgtat taggatatga ctttttgact acgcatgttc cagaaaaagt
     5521 aattattcga tttgataaag aagtagagta cgggaatgag atcgaaagcc attttgaaca
     5581 aatccctatc gaaaatggtg tcaaaacaaa acatgaaatc cgaatgcaag aacagctgta
     5641 ttgtgaagca aatattatat ggaaaaataa ataaaaaaga ggttgtgaca gaagggggca
     5701 acttcaagaa ataagaagaa atttacgaaa attgtttctc aaatttttgt aaattttcga
     5761 cttagttttc gaagaagcta tttttgtcac agcctctctt tttaagttta gctattagat
     5821 aatgatgttt agttttggat aaacgaaatc aattcttcac ccgacaaacc atagtttaca
     5881 gcaatcaata gttttaaacg tgctttttga ctattaatac tgttacagaa aataatcccc
     5941 attttttcta gttcttttcc cccgccttca taatcataaa caggctctgc gattccattg
     6001 aaacaacgag aaacaagtac tacagggatt ttttggacaa gaagcttttc caaagcagct
     6061 aaggcttgtg gagggagatt ccccgcacct aaagcttcga ttaccaaacc gtctactttc
     6121 gtatgggcga tggcttccaa taagtcccct tgcatcccag catatgcttt aacgatcggg
     6181 atagtcccat ctacagcgga aatgtccagt cgctttgttt ctaaaagttc ctgcaagaat
     6241 aaaattcgat tcttagttac taagccgatg ggacccaatg taggtgttcg gaaagtagca
     6301 acatttgtgg tatgggtctt tgtcacgtag cgtgcagaat ggatctcatc gttcatgacc
     6361 accaaaaccc ccttatctag cgcttcttca caacttgcga cacgaatcgc actttcaaaa
     6421 ttataaaggc cgtcacttcc aagttcatta cttgaacgca ttgccccagt caaaacaatt
     6481 ggtaatttgt tgccaatcgt tgtatcaaga aaaaaagcag tttcttctaa tgtatctgta
     6541 ccgtgtgtga tcaccactcc ggatatacct gatgcataag cggacaagat acgttctttt
     6601 aataccaaca tgtgagaagg catgatgtgt ggtgagggaa gattgaagat atcttcaact
     6661 aataattcta catgcttggg gattgtgatt tctgcatcta ataatgggtt tgccacatca
     6721 ggactaacac cgcccgtgag catgttttct ttcatggcga tcgttccgcc ggtgtgtagt
     6781 acaagtattt ttttcatttt acagacttcc ttacgaataa ttttgcatag ccgttaaatc
     6841 ttattgtata ctatataaaa gagttttgct atcagtgaag aataacgaat agcctaactt
     6901 atcataaaaa agctgacctt attaaaatgg aaaagactca accttgacct cctaatacaa
     6961 tggagtatct ggttgagttt cttttacaag aaagcagcca aggaaatcaa tcacgaaggt
     7021 ggccggaaat gatcagaatc ggattaacat cgtttaaaga acatgacaaa ttaactggta
     7081 aaaagaataa cactctttat gaatatgcag ggtttctacc cttagtggaa ttagatactg
     7141 cctattatgg aattccccga aagtcctcag ttgagaattg gataagtgaa gtacctgaat
     7201 catttcgctt tgtagtcaaa gtctatcaag gaatcagcgg tcaaggagac tggaagcctt
     7261 attatcagac cgaagaagag atgattgcgc attttttaga tgttatgtcg ccaatggtag
     7321 atgctggaaa gatgttttgc tttttagtcc aattcccctc atcattcaaa tgtacgaaag
     7381 aaaatgtcca gtacttgcgc aaacttagaa agtggtttga gggttatcct gtttctatag
     7441 aattgcggga ttattcttgg tacagcgatg aatttcttga acgtacaaga aactttatgt
     7501 ctagagccgc tttttcttta gccatcatcg atgagccaca gttgctgaat acaacgattc
     7561 cttttgatcc atttgtcacc aatccgaact ttgctctttt tcgattccat ggtaggaatg
     7621 cgcaaggatg gcaagcaaat gataaagagt ggcgtaaaaa aagaacgtta tatcgttatt
     7681 cagaaaaaga actgcaagaa ctagcacatg aagtcaaagt agttgattct aagacaaaag
     7741 aggtaggtgt gatttttaat aataattctg gtggtgatgc ggctgaaaat gcattagcac
     7801 tgcaaaaaat attaaatatc aagccagatg gactcaatcc aacccaaatg gatctgtttt
     7861 aagggcttat tttattcaag aaaggggcga agtctatgat caaagctatt ttttttgata
     7921 ttgacggaac attggtcacc aatcgttcaa aagcgttgga atcaacaaaa caagcaattg
     7981 aatatgctcg gaaaaacggc attttatgcg gtgtagcaac ggggcgcagc ccagtaaaga
     8041 tcaaagaaat cattgatgaa ttggaattag atatgtatgt cgtgtataat ggtcaactgg
     8101 tttttactgc agatcgcacg attatcgacc acccttttga acaaaaagta ttggaacata
     8161 ttgttgaatt tgcagatgag aaccatcgac aaatcgtttt tggtgcgaga aatcggttag
     8221 atggatcaac gacgatgcta ttaggacaat cgatttttat caagcgacta gtcagctttt
     8281 taccgcgtaa attcccggta cgtctgatga aaaaaatcct ccaggttttt agccctcacc
     8341 gacaaaagga tcgttatgaa acgttagaga tattaaaaga accaatttat caatgtatat
     8401 tgctcagtcc ggagtcagaa cagaaaaagt tagaacatcg ctttcccgag tgtacgtttc
     8461 agcgttcaaa tacgtatact gtagatatca tcccaaaagg tggttcaaaa ttactaggca
     8521 tccaagcttt cgcaaatgct gtcgaaattg aaatggaaga aattatggca ttcggcgatc
     8581 attacaatga tatcgagatg ctgaaaggtg taggaattgg agtggccatg gggaatgctc
     8641 agatcgaagt aaaacatgct gcagactttg tgactcaatc aaatgaagaa gatgggatat
     8701 acgaaggact gaaacacttc ggtctgattt attagtcaat gaaagaagga aaaaatcagt
     8761 atggaagcag agacaccttt tcaaaaagta gaaacattcc acaaaacttt tgatccaaga
     8821 agaccagaga tacccacggc ttttacagaa aatgaagctc atacacgtgc tgcgttcaaa
     8881 atagaagaac ttgttgaatt tgttgcagct acttcaccta ataaagaaca actgcatgaa
     8941 gctgttatgc tgctgcacga agcattagat aaagcagaag aaaaaatact tggtaaaaca
     9001 cagtggggaa gtgatccgct ggttgaacaa gtagatgcac tgacagatat cctttacttt
     9061 acttatggtt ctttttcatt actaggaata gtgcctgatc gaatattcga aatcgtccat
     9121 caggcaaata tggggaaatt atttccagat ggaaaacctc attatgaccc tgtaacaaat
     9181 aaagtgttga aacctgaaaa ctggcagatg gaatatgctc cagaacctaa aatcgcggca
     9241 gaaatccaac gtcagattca agaagcgatt gctaagaaga aatccgctga tataggcatg
     9301 tgcacaaatg aaaaagagtg atcagagttt cactattttt gttatcttta tacgatggaa
     9361 aaacaaaaga ttttcgttta agttagtgaa tgcatgaaaa tagtatgcta taatctagaa
     9421 tagatgtctt ttgcacgaga gacgtattat ttggatttat gccaagcaga acgccatctg
     9481 ttgcataaaa gatgcatact gaaaacggag gaaccataaa tgggaaataa tgttatcatc
     9541 ggaatcgtca ttgcaatcat tatagtcgcc attgtcgtct ttttggttag ttacttgatg
     9601 aagaaaaaaa accaagatcg attggatgcc ttagaaaaac gcaaggaaga attatttgac
     9661 ttgccagtga ttgaagaagt cgatgatgtt aaaaaaatgc atctggttgg gcaaagccaa
     9721 aatacctttc gtgaatggag tcaaaaatgg aatgacatat ctacttcctc gttcgctgaa
     9781 ctagaaagtc aaattttcga agtggaaaac ctcaatgaaa gttttcggtt cttaaaagga
     9841 aaaaaagcag ttgttgaagc agaagctaca atggaccaaa tggagaaaca agtgacggaa
     9901 atccgtgaag gtctaaagga attgcgggaa acggaagaac gcaactcact agctgttcag
     9961 caggcacttg acgtctatga agaactgaaa gaaaaagtca gagaagacga gcatgaatac
    10021 ggaccagctc tttttgaatt gcaaaaacag atcaaagtga tcgaaaaaga atttacgcag
    10081 tttgtgactt taaacacttc tggagaccct gttgaagccc gcgaggtttt ggataaagca
    10141 gaaaaacata cgtatgaagt ggaagctctg atgaaaaaaa ttccaccgct gtatgaggag
    10201 ctccatacca ctttccctga acaacttgaa gaaatcagtg atacttacga gaaattgatg
    10261 gaagaacaat atgtattccc agaagaagat cttgctgaag atatcgaaaa agtaagtcga
    10321 agaattgaaa attcattagc caatcttgaa aaaacagaag ttgaaacggt cgaatttgaa
    10381 aaccgtgaaa cagctgattt gattgattcg ttatatgata tcttagaacg ggaaatggaa
    10441 gctcagagat atgtaaaaac taaccaatca acgattgcag agtatatcaa acatacaaca
    10501 aagaataacc gtcaattatt gattgagctg gatcacacat ctcaaagata tacattgaac
    10561 cacaatgaaa ttggccgtgt acgaggtttc caaaccgaag tggaagaaat ggaacgtcaa
    10621 aatgaacaga tgattccaca gatccgtcaa cacgaaattc cttattcaga agtgcgcacg
    10681 ttctataaaa cagtcttcaa agtcttagaa gatatcgaaa agcaacaagt agagatcgac
    10741 gactcattac atgaattgag aaaaggcgaa aaagaagcgc aagaaaagat cgatacattc
    10801 gaattcaaat tgcgcagttt gaaacgtttt gtagaaaaac aacgtttgcc tggcttgccg
    10861 aatgattacc tagaattctt ttttgttgct actgaccgta ttgaagaact aagtgttgtc
    10921 ttgaataaaa ttcgagtaga catggaagag gtcaatcgtt tagttgctct atgtgaagaa
    10981 gatctagaat tattagataa gaagacacat gatttagtag atgcagcagc attaacggag
    11041 caaatgctcc aatatgcaaa tcgttatcgt catacacacg aagaagtgag agaagcaatt
    11101 gataaaagct actatttatt caataaagaa tatcactatc aagaagcatt agacgaaatc
    11161 ggtacagcat tagagcgagt agaacctggt gctttcaaac gcatcgaaga tttttatttt
    11221 aatcatccag acttagtata aagcaaaaaa gttagtaaac cagcaagagc aagacggatt
    11281 attgtcttgt tcttgtcttt ttttgtatat ttgaaaattg aaacaagtga aaatttcacc
    11341 tgctcttgaa agcaccttgg acaaccgtta taatagaaaa gaatttcaaa aaagaggggt
    11401 ttcttatgat atattttgat aacagtgcaa caaccccaat ctttccgcaa agcttagatg
    11461 catatgtaaa aaccagtcag cggattattg ggaatccatc aagcttgcat gatttaggca
    11521 atcaagctaa ccggttgttg cagcaagcaa gaaagcagat cgctgatttg atccatgtag
    11581 aaccagaaga aatctatttt acttcaggcg gaacagaagg agataactgg gtattaaaag
    11641 gaacgatgat cgaaaaaaga gaatatggga atcatatgat catttcagga gtcgaacatc
    11701 cggccgtcag cgagacagca gaacaattaa aagaattagg aatcgaagta agtatcgccc
    11761 cagtggataa acgcggattt gtacgtgtcg acgaattgaa agaattaata agaaaagaga
    11821 cagtattagt ctctgttatg gctgtgaata atgaagtagg atcgattcag cccatccagg
    11881 aaatcagtga tcttttggat gattttccta aaattcattt ccatgtagat gctgttcagg
    11941 cgattggaaa agtaccattc tcgcaatggt tgacagaaag agttgatttt gcaacatttt
    12001 cagctcataa attccatggc ccacggggaa ctggtttcat ttattggaaa aagggtcgtc
    12061 gtttagcacc attactgaca ggtggagggc aagaaagaaa tcagcgaagt gggacagaaa
    12121 atgttccagg tattgtggct atggttaaag ctttacgttt atcattagag ataaaggaac
    12181 aaaaacctga gcagacacaa cgcttaaaag tagctctaat caatgcgttg aagacgtatg
    12241 agaaagtgac gattttttca gaaggaactg actatgcacc gcatatttta tgttttgctc
    12301 taaaaggaat tcgcggcgaa gttctcgtcc atgccttaga agaaaaacaa atatttgttt
    12361 ctactactag tgcctgctcc tcacgtaaac atatggcaag cagtacattg cacgcaatgc
    12421 atgtaccaaa tgatctggct acatctgctg ttcgaatcag cttagatgaa aacaattcgc
    12481 ttgcagaagt cgagcaattt atgattattt tcaagcagct ttataagaaa ttttctgcaa
    12541 tcaattaaaa gaggagtgtt cttgtgaaat acagtgaaat tatggtccgt tatggggaac
    12601 tttcgacaaa aggaaagaat cgaaaaagct ttatcatgca actagcacaa aatgtcagac
    12661 aagctttgaa agactttcca gaaatcaaaa tccatgctga tcgggaccgg atgcatcttt
    12721 tattgaatgg cgcagacggg cagctagtta ttccaaaact agcaaaaata tttgggatcc
    12781 aaaatttctc tccaagtatt cgtgtagaaa aagatgtcga agttttgaaa caagctgttc
    12841 aagacatcat gaaagaaatt tatactggga aagaaacatt taaaattatc gctaaaagaa
    12901 gcgatcacca atttgaattg acaagtaatg aattgaatca aacattaggt aatgctgttt
    12961 ttgatatttt cccgcatatc caagttcaaa tgcgtcaacc agatattcca ttacgtgtag
    13021 agattcggcg tgatggcgct tatctatcgt atgaaacgat caagggagct ggaggattgc
    13081 ccgtaggaac aagcggaaaa ggaatgctta tgctttctgg ggggattgat tctcctgtag
    13141 cagggtactt tgccatgaag cggggagtgg aaattgaagc tgttcatttt gctagtccgc
    13201 cgtatacaag tgaacaagca ctgcaaaaag caaaagatct aacagccaaa ctagcacctt
    13261 atgtgggaac gatccatttt atcgaagtgc catttaccga gattcaagaa gaaatcaaaa
    13321 agtcagtgcc acaaggatat tggatgacaa ttactcgtcg aatgatgttg cgtttgaccg
    13381 atgaaattcg ccgtattcgt catggtttag tcattttgaa tggtgaatca ttaggtcaag
    13441 tggcttcaca aactttgcaa agtatggtgg ctatcaatga agtaacgaat acgccaatca
    13501 ttcggcctgt agcgacaatg gataaacttg aaatcattga agtagcagaa cagatcgata
    13561 catttaatct ttctatccag ccttttgaag attgctgtac gatttttgct cctccacagc
    13621 caaaaacacg ccctcaactg gaaaaggtcc atcaatatga agagcgttta gcaatagatg
    13681 aaatgatcca acgagcacta gctggcttga agatggaaga gatacaagca gaatcgtcta
    13741 atgaaaaaac tgaagaattt tcagaattgc tataagaggt tatgaaaata gtgttttgac
    13801 cagagtatta gagaaacaaa acgaaaaaat agctccatgt attttaccgt ttttgaacta
    13861 atgtcgaaga ttagctgatt gaataccatt tatcaagaag ttatggaaaa gccggttctt
    13921 ttttactaac tgaaaaataa aattttattt agagatcaag ctacagttga taaatgatat
    13981 aaaaaagaga atttcccatg aaatagcgag gttgtgacag aagtgggcag cttcaagaaa
    14041 taagaaggaa tttacgaaaa ttgcttttca aatttttgtg aaatttcggc ttatttttcg
    14101 aagaagttac ttctggaaca ccgtgtatca gaggttgcga aaaagctgcc ctgcatcaag
    14161 taataagaac agccaaacaa aaatagttcc tcatatttta cgtttggttg agcttattac
    14221 cgcagatgca agcttttgaa caccgtttta tttgtctcac acccttttcc ttcctacttg
    14281 taaccttgct cccgtgttag aaacatgtta cactaaatat gtgaaattat taacaaggag
    14341 cgtcgttatg cgagaaaaaa cagatacact gccaaaagca actgcgaaaa gattaccttt
    14401 atatttacgc tacttgaaga tgctggatga ctcaggtata agtagaatca aatcaaatga
    14461 gttcagcgaa atcacccaaa ttccttctgc aacgatccgc agagacttct cgcaattagg
    14521 cgagctaggc agaagcggat atggctatga tgtccctttt cttatcgatg tattcaataa
    14581 tatccttaat actaaagaag aaaaacgaat cgcattagta ggatatggaa atcttggcaa
    14641 agcattgaaa cacaacaatt ttagaagaaa tgaaaatcta aatatcgtgt gcgtctttga
    14701 taatgatcca gcactgatca atcgagtaat tgatggagaa atgatctatc cgattgatcg
    14761 tttcgcggag atagccaaag caaaaaatgt gacagtagca atctctacgg taccaagtaa
    14821 atattcacaa tctgctattg atgaaattgt caaaggaaat gtaaccgcta tacttaattt
    14881 tgcgcctgac agagtaactg tcccagctta tgtcaatgtt caatacattg atttgacaac
    14941 cgagttgcag acgcttattt actttgatga gaattactca gaagtttttt cttaggaaaa
    15001 tttgagtagg cttattgatc ttgcgagaac tcgtcgtaat tctttcaaaa attttttagt
    15061 actaaatgaa gtggtttttt cacgaccact tgttgactaa aaaaaaaaac cattgtagaa
    15121 tgaagcataa cgctgaacaa gaggagtaac tggcacattc attcttagag agagaactat
    15181 ctgctgaaag gttctcaatg acaaccagtg aaggtagctt gggagtttct gtcttgaatg
    15241 gagtagagat agacgagtcg tgatcgttaa ctcacttgag aggcaataat gattgattat
    15301 tgcaatttag gtggtaccgc gtaaatcata cgtcctagaa tacgaaagta ttttaggacg
    15361 ttttttagtt taaaggagga aaacagcatg acggaagaaa ataacctatc aacaaaatat
    15421 aatccgcaag aagttgaagc tggacgttat cagcattggt tagaacaaga tttgtttaaa
    15481 ccgagcggag ataaaaaagc aaaaccttat tcaatcgtaa tcccgccccc aaatgttact
    15541 ggaaaacttc atttagggca tgcttgggat acgaccttgc aagatatgct gattcgtcaa
    15601 aagagaatgc aaggatatga tactttatgg ctgcctggta tggaccatgc aggaatcgcg
    15661 acacaagcaa aagtagaagc gaagcttaga gagcaaggga tttctcgtta tgatctaggt
    15721 cgtgaaaagt tcatcgatca agtgtgggaa tggaaagaag agtatgcgag ccacatccgc
    15781 gaacagtggg ctaaaatggg cttatcttta gattacagta gggaacgttt tacattagat
    15841 gatggattat ctgaagcagt acgtaaagtt tttgttacct tatacgaaaa aggtttgatt
    15901 tatcgtggtg aatatatcat caactgggat cctcaagcac gaacagctct atctgatatc
    15961 gaagttattc ataaagatat cgaaggcgcc ttttatcata tgagctatga actagcagat
    16021 ggatcaggcg tagtagaaat tgcgacaacc cgtccagaaa cgatgttggg agatacggcg
    16081 attgctgttc atccagaaga cgagcgttat caagcgctca tcggcaaaaa agtgattcta
    16141 cctttagtca ataaacaaat cccaatcatt gcagatgaat atgtagatat ggaattcggg
    16201 acaggtgtag taaaaatcac tccagcacat gatccaaacg actttgaggt aggtaatcgt
    16261 catgatcttc cacgcgtaaa tgttatggat gatgatggta cgatgaatga actagcagga
    16321 aaatatgctg gaatggaacg ttttgctgct cgtaaagcaa tcgtgcaaga tctgaaagaa
    16381 atcggtcgat tgattaaaat cgatacgatg atccacagtg tgggtcattc cgagcggaca
    16441 ggtgtcgttg ttgaacctcg gctgtctact caatggtttg taaaaatggc gccgttagcg
    16501 aaaaaagcca ttgataacca agaaacagat caagcagtgg aattcttccc tccaagattc
    16561 aatcaaacgt ttttacgttg gatggaaaat gtccatgatt gggtaatctc acgacaatta
    16621 tggtggggac atcaaattcc tgcatggtat cacaaagaaa caggtgaaat gtatgtaggg
    16681 atggaagcac cagcggatag tgaaaactgg gtacaagatc ctgatgtttt agacacttgg
    16741 tttagttcag ctttgtggcc tttctcaaca atgggctggc cagatacgga aagcgaagat
    16801 tataagcgat acttccctac tagcacttta gtaaccggtt atgacatcat tttcttctgg
    16861 gtaagcagaa tgattttcca aagcttggaa tttactggcg aacgtccatt tgagaatgtc
    16921 ttgattcatg gattgattcg tgacgaacaa gggcggaaga tgagtaaatc attaggtaat
    16981 ggaatcgatc cgatggaagt aatcgaaaag tatggagcag atgctctccg ctggttctta
    17041 tcgaatggtt ctgcaccagg acaagatgtc cgcttcagct atgaaaaaat ggatgcttct
    17101 tggaacttta tcaacaaaat ctggaatgct tcacgtttcg ttatcatgaa tatcgaagga
    17161 atgaccgtag aagagatcga tttatctgga gaaaaatcag tggctgaccg gtggatcctt
    17221 actcgtttga atgaaacgat cgaacgagta actgagctat ttgatcgttt tgaatttggt
    17281 gaagcaggtc gacaactgta taactttatc tgggatgatt tttgtgactg gtacatcgaa
    17341 atgagtaaag aagtacttta tggagatgat gctgtttcaa aacaaaccac ccgtagcatc
    17401 ttagtccata cacttgatca gatcctacgt ttattgcatc caatcatgcc attcgttaca
    17461 gaagaaatat gggaacatat tcctcatcag ggaaattctc tagtagtggc agaatatcct
    17521 gttgtacgtc cagagtttga tgatgaaaca gcttctaaag ggatggaagt attgaaagaa
    17581 ttgattcgag ccgttcgaaa tattcgttca gaagtgaata caccattgtc taaaccaatt
    17641 acgttgctaa ttaaaacaaa cgatgctaaa atcgatcaat ttttagttga aaatacaagc
    17701 tatatcgaac gcttctgtaa tcccgaagag ttgacgatat caagtgagat cactgcgcca
    17761 gatctagcga tgtcagctgt tctgacaggt gcagaaatct tcttgccgtt agcaggattg
    17821 atcaatattg aagaagaaat caaacgatta gaaaaagaac tggaaaagtg gacaagcgaa
    17881 gtaaaacgtg tccaaggaaa actttctaat gaacgttttg tttctaacgc gccggaagaa
    17941 gtagtagagg cagagcgtgc aaaagaaaaa gattatttag agaagcaaac ggctgtcaaa
    18001 gaacgaatcg aaagtttgcg tactattcaa taagaataga aaaagagagt gtgaaatcag
    18061 ctagctatta ctaaaagcta gctgattgaa catcgttaca ggaaagagtt tgagcctcac
    18121 tcttagcttc gattcataag gagatgtgac ataaattttg tcgcatctct tttttagtct
    18181 actcttatta tgtaggagaa ggagggaggt ggatcatgta tcaagagtca ataaagtagt
    18241 tgataaaatc gttcctctct ctgtttcttt tctctttgtc atacttttat ttttcttatg
    18301 tgttctattt gttataataa aagagaaatg ggggaattgt tgatgacgat tgaagaagca
    18361 atagaatgga tccatagtcg attaccattt ggttcacgtc ctggccttga ccgagtcgaa
    18421 gctttattag aagtggtagg gcatccagag agaaaagtaa aaacgatcca tatcgctgga
    18481 acaaacggaa aaggttctac tgtagcttat ttgagaagcc tattagaaga ggctgggctg
    18541 acagtaggta catttacttc accttatatc gaaacgttca atgaacggat cgcaatcaat
    18601 ggcaaaagca ttccagacaa tgatttgatc cagcttgtaa aaaaatatca gccactgatc
    18661 aaacaacttg atcaatttga agcagtagct ggtattactg aattcgagac attaacagca
    18721 atggcatttg attatttttt gatgaagaaa gtggacgtag cgattatcga agttggctta
    18781 ggcggcttat tggattctac caatgtagct gtaccaatgc tgacgggaat aacaacgatt
    18841 ggacttgatc ataccgaaat acttggagat acaattgaag aaatcgctgc acaaaaagct
    18901 ggaatcatca aacaaggcgt tccagtagta acaggaaata ttgccccaga agcttttact
    18961 gtcatccaac aaacagctaa agcaaaaaat gcaccgcttt atgtatacgg ggaaaattat
    19021 aaaataaact atctgcatcc tgatgagatg tggggagaag tatttgaatt ttttaatgaa
    19081 ataggaaaga tccctcaatt aaccactccg cttttagggc aacatcaagc tgaaaatgca
    19141 ggagtagcca tcgaattgta ttttgtttac tgtcaattgg aaaagctgcc tttccgagaa
    19201 aaagatgttt tgaagggatt gaagaaatct cgatggccag gaagaatgga gcgtttgagt
    19261 aacgagccat tagttgtttt agatggtgcc cataatacgc atgcaatgaa ccggcttgtt
    19321 gagaatttga aaaaagaatt caaaggatat caaatcaaca ttttgttttc cgccttacga
    19381 actaaaaatg ttagagaaat gctagaacga ttgcagcaag taccaaatgt ccatatttat
    19441 ttgacgacct ttgaacatcc aaaagccatc gaattaagtg aagtatcaga tttagcaagt
    19501 gagagagtat caatcgtttc attgtggcaa ttcggattag cagatatatt ggaaaagatg
    19561 acaaatgaag acgtattagt tgtgaccggt tcactttatt tcatttcaga agttcgccat
    19621 ttattaatac aattaggagg agatcatgaa gtataatgca gtgatttttg acatggatgg
    19681 tttgctgttc gacacagagt tagtgtatta cgaagccaca caagtagtag cagacaagat
    19741 gggttttcct tatgataaag acctgtattt aaaattctta ggtgtttcag atgaagaagt
    19801 atgggcgaat tatcatatga ttttctcgga atttggcaaa acaagtgtac aaagattcat
    19861 agatgattca taccaggaaa cgcttgaacg tttttcgcaa ggagcggtac aaatgaagcc
    19921 cggtgtgttg gaactcctct cttttttaga agaacatcag attcccaaag ttgtcgcttc
    19981 cagtaaccaa cgagcagtca ttgaactttt attgaagaag aacaatctgt ttgaacgatt
    20041 tgagacgatt gtttctgcag aaaatgttca acgagcaaaa ccagacccag aaattttctt
    20101 gttagctaat cagaagctag gtacagcgaa gaaagaaaca ttgattttag aagattcaca
    20161 aaatggtatt ttagcagctt ctgcggctga gatcccggta gtcatggtac cagatctgct
    20221 tccaccatcc caagaattga tcaaaaaaac agctggggtt gtttcttctt tacatgaaat
    20281 ccctgcattg ttaaaataaa aatccatctc ttttcgtatc ttattttaga taagatagaa
    20341 aggagatgtt tttttgggaa aacgattgat caaagaagta cctactagct ctttaccaag
    20401 ggaacgtatg gaaatttatg gcgcggaagc tttatcggat caggagcttt tggcaatctt
    20461 actgcgtacc ggtcaacacc cttatagtgt gatgtcgatt gctggaaatc tgttaaaaac
    20521 gtttggagga ctggcctctt tacgacaggc tactttgcat gagttagaag agattcgagg
    20581 gataggacgc gtgaaagcaa tcgaaatcaa agccttgatc gaattaggtc gacgaattca
    20641 gacggatcat gaaagagcgg ctccaccggt tcgtaccagc tacgaattgg cccagcaatt
    20701 gattctggaa atgaaagatc acaaacaaga acatttggtt tgtatttatt tggacacgaa
    20761 gaatcaagtg atcttaaaaa aaacagtgtt catcggttcg ctgaatcagt cagtcgctca
    20821 tcctagagaa attttccatt atgctgtacg ttattgtgca gcaaggatcg ttttagcgca
    20881 taatcatcct tctggtaatg ttattccatc gcagcaggat atgaatttta ccaaaaggat
    20941 acaaaagtgt ggagaaatga tggggatcac tgtactggac cacttgatca tcggacgcaa
    21001 acgatatttt agtttgagag aagaaggaat gatggaagag aagtaagttg ctttcttgca
    21061 aaaaaaatga agcaagtgta caattatatt gtaattttgt ttgggaatag aagttgaaac
    21121 atctgttgca cctctatcgt acacgagcaa tgttacaatt taaattagtg cttttggcac
    21181 gaggaggata ttattatgga aaacggaaca gtaaaatggt tcaacgctga aaaaggattt
    21241 ggatttatct cacgtgaaga tggaagcgac gtattcgttc acttctcagc tatccaaggt
    21301 gacggattca aaacattaga agaaggtcaa gcagtgactt tcgacgttga agaatcagac
    21361 cgcggcccac aagctgcaaa cgttgtaaaa gcttaatttt atctgtaaat caaattgatg
    21421 gtagacatca atttttacga gagaacaaag cgagtaacaa aaatacctgc cacaagagac
    21481 aatttctctt gtggcaggta tttttttgta tgaaaaagta aataaattta agaggctatg
    21541 aaagagccac cttgtatcaa ggaataagaa caaacaaaaa tggcttctca tgtttttcat
    21601 ttgttttctc ttatattcag gacaaggtgc tgatttcata acctcttatt tattcttcat
    21661 ctggtgtcat taccattggc aaaatcattg gatgtctttc tgtacgttcg aataagaaag
    21721 gctgaagcgc gccagtcatc gcttctttca atttatattc tgaacagtca ggtccattca
    21781 aacattctct taaagcgtgg aataagattc gctgagcttc attgatcatt tctcctgatt
    21841 ctcgcatgta gacaaatcca cgagagagaa tatctggacc agccaagatt tcttttttct
    21901 tgatatccac tgtcgcgaca gctaatacaa gaccttcttc tgaaagcagg tgacggtcac
    21961 gtaaaacgac attaccaata tcaccgatcc cgctaccatc gatatacaca tctccagcca
    22021 caaaatgccc agcaggacgc gcagaatcgc tagttaacgc taaaacatcg ccattttcca
    22081 tgatgaagca gttttctgca ggaacaccta catcttgtgc tagagaagca tgtactttca
    22141 acatgcggaa ttctccgtgg acaggaataa aatattttgg cttcatcaaa cgaagcatta
    22201 atttttgttc ttcttgtcca ccgtgaccgg aagtatggat gttattgatt tttccgtgaa
    22261 tcacttctgc accagcttca gacagcaaat tgatcaaatg attgacactt gttgtattac
    22321 caggaatagg tgaacttgaa aagatcactg tatcgtctgg ctggatttgg atttgacggt
    22381 gagtaccatt cgcgatgcgg ctcaacgcgg ccatcgcttc tccttgagaa cctgtacata
    22441 ggatcatcgt ttcattggca ggcaagcgat tgatttcgtg tgcatctaca aatgtgccgt
    22501 ctggaacttt gatatatcct aaacgttgac cgttgacgat ggcattttcc atgcttcgtc
    22561 cgaaaacagc gattttacga ccagttgcca cagcggcatc tgctgcttgc tgcaaacgga
    22621 agatgttaga agcaaagctg gcaaaaatga tacgtccttc gatttttgaa aagatcttca
    22681 aaatagaatg cccaatcgtt ttttctgatt tagtaaatgt tggaatctca gcatttgtac
    22741 tatcagataa aaggcacaag actccttctt ccccgatacg agccatttta tgcaagtttg
    22801 ctggttcgcc aacaggagtg aaatcgaatt tgaagtctcc agtcgctacg atatttccag
    22861 aaggtgtttt caccacgatc cctaatgcat ctgggatact atgggtcgtt ctgaaaaagc
    22921 tgacagaagt ctttctaaaa cggatgatcg tatcttcatt gatctcatgg agctctgcat
    22981 ctcgaagcaa gccatgctca tctaatttgt ttgtgatcaa cgctaatgcg agaggaccag
    23041 catagatagg aatatttgct tgtttcagca agtagggtat tccgccgatg tggtcttcgt
    23101 gcccgtgagt aatcaccaag gcttttactt tgtgtaagtt ttgtatgatg tagctgtagt
    23161 cgggaatgac atagtcaatt ccaagtaaat cgtcttccgg gaatttgatc ccggcatcaa
    23221 tcaaaatgat ttcatcttgg aattgtaccc catacgtatt tttcccgatt tcacctaatc
    23281 caccaatagc aaagacgcct gtctcgttat ttttgatgga aagtttcata ttaaaactcc
    23341 actaatgaga aatcagcatt ttctttttcg tactctagat gacttccttc tagaagttga
    23401 acgaattcaa tattgtatgg ggtattttct tccacacgtt ggcgagcgtc tacatcgctt
    23461 tttgcctcaa tgtaaagaga ttttgtttct tcccgtttag ggttgcgtac ttttgtttct
    23521 tgataataaa ctttatagat cattgttttg tctcctttat ctgcgtaaag cagtgattta
    23581 gctagttgat ttttttagac aacacaaaac gaggggacaa gccacctggt ttttcatatg
    23641 ctttgactag aatactggat tcgagtcaaa gagacaagcc tcgtttttgc ttatctgttc
    23701 ggatattttt tcaaccgtac cttctgtata aaaaatatac aacaattaat tgttattcta
    23761 gcataaaaca gcaaataagt atagaaatca actagattga aagaaaaaat caatgtttat
    23821 catcccgcta taatcataat gactttttta tgagagaagt gttctattaa taggctcgtt
    23881 gtgctatcat ttttagtaat gaaaatgtaa caaaagatta aaaggagatg agaaagtgaa
    23941 actaatgtgg cattatacca tgcgttataa gaaattcttg tttttcaatt tcatatgcgt
    24001 atttggtttt attttgattg aattaggact tcctacgatt ttagcacgta tgatcgatgt
    24061 aggaatccgc aatgatgatt ttgattatgt gaagcaacaa gggatattta tgatcgttat
    24121 tactgtaatc ggtattattt tgaatatctt gttaggttat tttggttcga ggattacgac
    24181 taatatcgta gcagatattc gagatgattt gttcgaaaag atccaaagtt attcgcatca
    24241 ggaatacgaa aatctaggcg tatcttcatt gatcactcgt acaacaaacg atgcttatca
    24301 gatcatgttg ttcctgcaaa atattttacg tatcggtttt atggcaccaa tgatgttcat
    24361 tgtcagtttg tacatggtca tgcgtacaag tgtgacactc agtttgtatg tagtaggtgc
    24421 attaccttta ctattattgg cagtcgtagc gattgctaag gtgtctgaac cgttatctaa
    24481 aaagcaacaa aaaaacttag ataaaatcaa cagtatcttg cgggaaaatc tttcaggatt
    24541 gcgtgtgatt cgtgcattcg tcaatgaaaa gtttgaagaa aaacgttttg ctgtagtcaa
    24601 tgagaattat gcttctagtt caaaaagctt attccgtttg atggctgttg cacaaccggg
    24661 tttttatttc ttatttaata tcgtaatggt attgatcatc tggagcgggg cagtgcaaat
    24721 cgatgcaggc gagcttcgtg taggagattt gattgcgttt atcgagtata tattccatgc
    24781 cttgttctca ttcatgttat ttgctagtgt gttcatgatg tatccaagag cagctgtttc
    24841 agctggacgt atccaagaag ccctagatgc caaaccttcg attgtagaag atccaaatgg
    24901 tatatctgat acaaaaacta aagggtatat cgaatttcaa aatgtaacat ttgcttatcc
    24961 aggccattcg caagagccag tgatacgaaa tgtcagtttt acagcctctc caggagagac
    25021 cgttgctttt attggaagta ccggtagcgg aaaatctacg ttgatccaat tgatccctcg
    25081 cttttatgat gtgagtgaag gaaagatctt gatcgatgga gtagacgtga gagattatcg
    25141 gctaagcaag ttgcgagata agattgggtt tattccgcaa aaagcgctgc tatttactgg
    25201 aacgattgca gataatttgc gatacgggaa agaagatgct acgcaagaag aaatggaacg
    25261 tgcagctgag attgctcaag cttcagaatt catttctcga aaaccagatg gatttaatga
    25321 acatttatca gaaggtggtt ccaatttttc aggtggacaa aaacaacgat tggcaatagc
    25381 cagagccatc atacgccgtc cggaaatcta tatttttgat gacagctttt ctgcattaga
    25441 ttatcaaacg gatgcgaagt tacgtgctcg tctgaaaaaa gaaacgacag aatctactgt
    25501 attgatcgta gcacaacgtg tgggaacgat catgcatgca gataagatca tcgtattaaa
    25561 tgaaggagaa gtcgtcggta tggggacgca tcgggagttg ttggaaaaat gcccgattta
    25621 ttatgatatt gccgcttctc aattatcaaa ggaggaatta gcatgaaaaa aaccttttct
    25681 tccttaaaac gtttagctcg atatatcaga ccgtatcgtg agacttttct tttagtgatc
    25741 ctatttacag ttctgacagt ggctttcaac gtggctttgc catatgttac aggacttcct
    25801 actacagaaa tcagccgtaa tattgccaat ggcgaatcaa tcaatttttc ttatatattc
    25861 aaatgtctca tttggatcac agttgttgga atagggtatt gtatttcgca attactttct
    25921 ggtgttttga tgacaaatgt cgttcaaagt gccatgcatg atttgagaag agacatagaa
    25981 gaaaaaatca atcgtcttcc ggtttcatat ttcgataaaa accagcaagg aaatattttg
    26041 tctcgtgtga cgaatgatgt agatgcagtc agcggtgctt tacaacaagc ttttattggt
    26101 atcgtaaatg cgatattggg aattacgttg gctgcagcaa tgatgtttta tatccagccg
    26161 atgatggcgc tgatctctat gatcatgatt ccaacatcga tctggatctc aaaacgagtg
    26221 atcaatgctt cgcagaaata ttttcaaagc atgcagaatt ctcttggtga gctaaacggc
    26281 tacgtccaag aaaatatgac aggttttagt gtattgaaag tatatggacg cgaaaaagaa
    26341 acattggaag gattcaaaaa agttaatcac agcttgaaat actttgggtt tcgtgcagca
    26401 ttcatttctg gcttgatgat gcctctagtt caattaactg cctatgctac ttatattggt
    26461 atggctgttc taggaagctt ttatgttatt actggggcga tcgttgtcgg tcaactacaa
    26521 gcattcatcc aatatatttg gcaaatcagt cagccaatgg ggaacgtgac acaattgtct
    26581 tcagttttgc aaagtgcatc agcggcaacg aaacgtgtat tcgagatttt agatgagcca
    26641 gaagaaaaag aaaacaaagt agatgcttca ctgccagaaa cgattttagg agatgtgaca
    26701 ttcgatcatg tcgattttgc atatgatccg aagaaaccat taatccaaga tttgaatttt
    26761 gaagtgaaag ctggtcaaac cgtagctgtg gtcggtccga ccggtgccgg caagacgaca
    26821 ttgatcaatc tattgatgcg tttttatgac gtagacaaag gagcaataag gattgatgga
    26881 atcgacacca aatcaatgag tcgcagtgat gtgcgttcgc tatttggtat ggtcttgcaa
    26941 gatgcatggc tttatcaagg aacgattgca gataatatcc gcttcggaaa gcttgatgcg
    27001 acagattatg aagtagttga tgctgcgaaa acagcaaacg tagatcattt tatccgaacg
    27061 atgccagatg gatatgatat ggccatcaat tctgagggag acaatgtgtc tttaggacaa
    27121 aaacagttgc tgacgattgc ccgagcagtg gtctccgatc ctaaaatctt gattttagat
    27181 gaagcaacaa gttcagtaga cacccgttta gaagcgttga ttcaaaaagc aatggatcgc
    27241 gtgatggagg gacggacaag tttcgtcatc gctcaccgtt tatcgacaat ccgtgatgca
    27301 gatctgattc tagtgatgga tcaaggtcag atcattgaaa aaggaacgca tgagagttta
    27361 ttggcacaag gtggatttta tgaaaaactg tataacagcc aatttgctga ggaagcagaa
    27421 taagtcatat aagaataaaa cagtttaaaa cttatttttt gttctctatt cttgataatg
    27481 gtaaagtaaa gttatcaaga aaagtgagga cacggagatg gaacagtttt taaatgaaaa
    27541 aattactgaa gccaaaagag aagttgatcg tttaacgcac gtctgtcaga cgaagccttt
    27601 tgattcagct gatttttttg aaaatgagtt tcaaatcgaa cgcttggcag cacaaataga
    27661 agtgtacgaa ttaattttaa aaaaagtaat agaagaaaat atagatgtac aataaaaaca
    27721 ggtaaaacgt cagttaccag atactgtaaa tagacatctg gcctgagcag gttttacctg
    27781 ttttttttct tatgaaataa atatatgcta tgataccttt attcaatagg attagtttct
    27841 acaaaaaatg aaaggagaaa aataaatatg gaacggttta tagcatcaaa tgccacagtg
    27901 attggtgatg tgacattgtc agaagatgtg acgatctggt atcaagctgt tttacgcggg
    27961 gacagtaatt ggataaaaat aggacaaaga acgaatatcc aagatggtac aatcatccat
    28021 gtcgatcatg atgcacctgt agatattgcg gagaatgtga cggtaggaca tcagtgcatg
    28081 ctacatggct gtacaatcga aaaaggggcg ctgatcggga tggggaccac gatattgaat
    28141 catgcggtta ttggtgagaa tagtttgatt ggtgctggat cattagtaac agaaggaaaa
    28201 gttattcctc ctaatgtctt ggcttttggt cgcccagcta aagtgatccg tcctttgaca
    28261 aaagaagaaa tacaaaaaaa caaagaaaac atccagcatt atgtcgagat aggtcggatg
    28321 catgcgcaag gaaaattcaa ggagcaaaga taggcagtag catccataaa ataaaaaagg
    28381 atggtcagtt tgagttgacc atcctttttt atttggttta gataagagag cttaaaaaag
    28441 cttcctttga aacagattaa tttaatgaag aactgttttc catgacttca tcgatcaatc
    28501 catatgcttt tgcttgttca gctgtcatat agttgtcacg atctgtatct ttttcaatca
    28561 cttcaagcgg ttgtccagtg cgttcagcta aaattttgtt taagcgttcg cgtgtttgca
    28621 aaatatggcg tgccgcaatt tcgatttcag ttgcttgccc ttgagcgccg ccaagaggtt
    28681 gatggatcat gatctcagca tttggtaaag caaatcgttt accttttgta ccagctgtca
    28741 gcaagaagct tcccattgaa gcagccatac ccataacaat tgtttggaca tctgctttga
    28801 caaagttcat tgtgtcgtaa atagccatac ctgctgtaac tgaaccacca ggagaattga
    28861 tataaagata gatatctttt tcagaatctt gagcgtctaa gaaaagcaat tgggcgatga
    28921 tcgaattagc aagatcatct gttacttggc cgcttaacat gatgatacgg tctttcaata
    28981 aacgtgagta gatatcgtaa gctctttcac ctcgtgaaga ttgttcaatg actgtaggaa
    29041 ttaaattcat aatagagttc ctccttgatt tcgtttagaa atagtgtacc ataatttagt
    29101 ttctaatacc taaaatgtag tatacatttt tggtcaaaaa aggtcaaata aaaaacttag
    29161 gcaaattagt cgcataaacc cgctttatgg tatgataagc agagaaacaa aacaaggagg
    29221 aacaacagat tgatagttgg tattactggc cttatcggag tgctgacagg tgctgtagtt
    29281 gtctatatcg gcatgatact gcaaatgcgt cacttggaaa ataaagaaca gcgtagacgt
    29341 gaattggaat tgaaagtcaa agaaatcgag acgctgaatc aattaaataa aaagatcaac
    29401 gagatcttgc aaaaacggac aacgctgatg gaagaatatg tcagctttga tgcatttgat
    29461 gattgttata tcacgattga cgattttgcg tatctccaat cattttcagc gcaaaatagt
    29521 ttttatctgc caaattatat tttagacgag ctatttaaga atatctccca tcgaaaagtg
    29581 attttatctc cagatgaaac aatgaagatt ggtggatatg cttataaagg cggacgcgtg
    29641 attttagaga attttacgga tagtctgact gaaatgatca atgaaaaaaa agctcagttg
    29701 aagactatga cgcgccagcc gattggtttc ttctcgaaag atgatgtttg gagctgatat
    29761 tttgaagcat tgccgttgca catgagagcg tggtaatgct ttcttttttt cttaatgata
    29821 caatagaact gaacggagtg aagaaagatg ttacaaatct atgcccatcg cggaagcagc
    29881 ggcacccacc cagaaaatac gcttcctgca tttgcagaag ctgtacgagt aggtgcagat
    29941 ggtatcgaac tagatgtcca tctaagcaaa gatggttatt tgatcgttat gcacgacgaa
    30001 gaagtggacc ggacgactaa cggtaaaggg ctgattcgag aaaaaacatt ggaagaactc
    30061 aaaaaattga atgcaggggg caaattcagc aaagaatttc cagcagctaa agttcctgaa
    30121 ttgagagaag tgttaggact gctcctccaa aaaaattacc gagggatgct aatgattgaa
    30181 attaaaactg atcagtatga atatcccggt atcgaagaaa aattagccga aacattaaaa
    30241 agtagagatt ggccttttca acatgcatat tgcagctttc atttggattc tttggaaaaa
    30301 atcgcattac tggaaccgga gatacagctg gacttattga tgagtacttc tgataaaaag
    30361 atccaactag cagcagaaac cccttatata gaaggagtac atccaaaaaa tacttggttg
    30421 ttcgaacatc cagtccaggc agctgatttt ccaaaagcaa ttcgtgcatg gacgattaac
    30481 gatgaagaag aaatggccaa atgtcaagaa ctcaaaatag atggaataat aactgatttt
    30541 ccggagaaag ctcttcggtg gaaacaatca acgaaaagta ggtaaaatat ggaaaaagta
    30601 ttagtgatcg tcggtccgac agcagtcggt aaaacagcgc taagtgtgga actcgcacaa
    30661 ctatttggag gagagatcat cagtggtgat tcattgcaaa tctataaaaa tctggatatc
    30721 ggtacagcaa aaatcaaaga agaagagatg aataatgttc cgcatcattt gattgatgtg
    30781 atcgaaccta ctcaacgtta ctcagccgct gattttcagc attcaggtcg acaattgatt
    30841 accgagattg cgaaccgagg gcacctacct atcatagctg gtggtacggg tctctatatc
    30901 caatcccttc tatatgatta tcagttagga gcgacagaag aagcgaatcc tgagattaga
    30961 aaaaagtatg aaacgttagc tgaaacaatt ggttcccaag cattatggaa tcagttgaaa
    31021 gaaaaagacc cggcagctgc tgagaaaatc cactggaaca atcaacgcaa agtgataaga
    31081 gctctggaag ttttagagac gacaggacat agtatcacgg cacctaaaga agagccaaag
    31141 caattgtatg attacctcat gattggatta gatacgaagc gtagtattct ttatgaacga
    31201 atcaatcaaa gagtcgattt gatgctagca gaaggtttag tagcagaagc gcgtaaggtg
    31261 tatgagcttg gagaagtcca ggccagtcaa ggtattggat ataaagaatt tttcccttat
    31321 tttgaaggga aagaaacatt ggaagaagca acagaacaag tcaaactaca ttcgagaaga
    31381 tacgcgaaac gtcagttgac gtggtttcgt aaccgtttgg atgcacagtg gtttgatctt
    31441 ataagtgagc caaatcaaaa aagtcagatt gagcaaacaa tcaaaaaatg gctggaggcg
    31501 gaataaaatg gaagaaaaag tgatcatcgt aggggtagaa accgaagcaa accagcgtta
    31561 cttttcagag tcaatggaag agctagttca gctgacgaat accgcatcag gagaagtcgt
    31621 attcacaatt acacaaaaga gaccacaagt ggatcggcaa acgattatcg gtaaagggaa
    31681 attacaagaa ttagttcaac aagcggatgc gcatgaagcg gatttgatca tctttaatca
    31741 tgagctaact ccgagacaga gccaactgat taccgatgct gttggtcttc cggtaattga
    31801 tcgcgtgcag cttatcttag atatttttgc gatgcgtgct cgttcgaaag aagggaagct
    31861 gcaggttgaa cttgctcagt tagaatacct gctgcctcgt ttagtagggc aagggaaaac
    31921 attatctcgt cttggcggtg ggatcggaac aagagggcca ggtgaaacaa agttagaaac
    31981 agaccgccga catatacgga ataaaatttt tgctgttaag aaagaattga aagaagtaga
    32041 agctcatcga gagcgcaatc gccaaaaaag aaaaaatagc gagatttttc agattggact
    32101 tattggctac acgaatgccg ggaagtcaac tatcttgaat ctattgacac aagcggatac
    32161 ttattcgaaa gatcaattgt ttgcaacgtt agatccattg actaagagat ggcgttttgc
    32221 tgaaggattt gagattactg taacggatac cgtcgggttc atccaagacc tgcctacgca
    32281 actgatcgat gctttccatt ctacattaga agaaagtcaa aatatggatc ttttgctaca
    32341 tgtagtcgat gctagttctc cagatcgtat tttacaggag cagactgtct taaaattgat
    32401 ggatgaactg aatatgaaag aaatgccgat actgactgtt tacaataagg cagaccagat
    32461 cgacccagcg atgttcacgc ctacgctatt cccaaatgta ttgatttcta ctcagacgaa
    32521 agaaggtaag atggcgttga tcgaagcaat caaacgtcag ctgatggaac taatgacccc
    32581 ttatacactt tctgtgccaa gcaacgaagg acagaaatta agcgagttga gaagacaaac
    32641 aatggttttg gacgaagcgt acatggaaga aacaaacgaa tatgaagtta aagggtttgc
    32701 aaaaaccaat tcaaaatggt taagaaaaaa ccaataaaat tttatttttt taa
//
LOCUS       DAZABD010000031        32410 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000031 DAZABD010000000
VERSION     DAZABD010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32410
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.031"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1218)
                     /locus_tag="ACHALG_001465"
     CDS             complement(<1..1218)
                     /locus_tag="ACHALG_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN2586881.1"
                     /translation="MQTFLTKTSFKKVVLLDYLLEKDNWCAMEELRNLLNVTEKSVLH
                     YIEELEDLFKQYNGNILLKNEDNKRFFIKKEKDFPIYNIYLHFYKASYNYHLIDFMYK
                     YPRSVLKDFAKEQFTSVSTVFRYAKLLIPYFRRYHITFHPFQLELNASEANIRSFFYY
                     FYWNSTRESSDKWPFHIEQKEIEKYIVAFEGIYDITLTIFQKRVFSFWLAINIERSSF
                     RKVRVDNEYKSVISDDPHFNLLKKWSKQINLSFNSDELCFLYRIIYSFGVIDGNAIYE
                     NSHAYAHQRQNTCSYRAVENLEKVLQSMFRFSLDIKDPELIFNFIAFHERSYLFYGNP
                     DLFFNRSYIEEMKEEEPRTYHIMEKLKKELQANADLDVSKKLENWAQLFLDYYYVLDY
                     YDLFLTNVKPIKIL"
     gene            complement(1999..2352)
                     /locus_tag="ACHALG_001466"
     CDS             complement(1999..2352)
                     /locus_tag="ACHALG_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2586882.1"
                     /translation="MNSFKKKFVSRFLNMKNFFEKKGDYDAYNYVLNLEKQTYYTLRK
                     NDDNDFWTMLPKMLSIDSKLSLLEEFINLDIEEFCGGKELINMVESDYHTYYKESCGY
                     KVDEQCPQSIIFYIE"
     gene            complement(2984..4609)
                     /locus_tag="ACHALG_001467"
     CDS             complement(2984..4609)
                     /locus_tag="ACHALG_001467"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFN2586883.1"
                     /translation="MKKIYSKEIDCFRGIAVLAVIFYHLPNSLLPGGLQGVTIFFVVS
                     GFLMAKNTIRDEKNRQFSFLHFYYKRIKRIYPFLLFSLVVFIFILGISNIRLIGNIRS
                     ELPSLIFGYNNWWQLNQGVSYFDSYFNASLFKHYWSLSVELQFYLIWPFLFIVIKRMR
                     RKQVFYLIYTLIFVSILFSLFLPSTKAYYHTIAKLFPFLLGVWGYFNRITIGRFFEQN
                     TFSKIWLLLLASLCLILFPIFPYTLNELLISICFALLLASVDDMNIAKKTKIASTKLS
                     FVGKISYELYLVHFPVLIALTNLFKHHHWKYLDIAGLFTVFLCTLLMITCFRKGVQKM
                     RSPKIWTAMALMIIGVSVLVLRAPASRLTADQNQLQSLLKENSSKANAGENLLSQEKK
                     ILFIGDSVMLGAYQELKETFKKEALVDAKESRQITALPEILKEHKDLVSYQQIVIGLG
                     TNGVITDEAIEQTMSLLKDKKVYWVNIKAPTGWQDTINATLATLPNQYSNITMIDWYS
                     ESKNHPEFFYDDETHLNESGRTAYAKYIASVIQ"
     gene            complement(4895..6307)
                     /locus_tag="ACHALG_001468"
     CDS             complement(4895..6307)
                     /locus_tag="ACHALG_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HFN2586884.1"
                     /translation="MRAKVQAIGGFLTNMVLPNIGAFIAWGIVTALFIPTGWFPNESL
                     NELVSPTVSYLLPLLLAYTGGRMVGGTRGAVLGTIGTIGLIIGADIPMFMGAMIMGPL
                     GGWMMKKTDDFLDGKVPAGFEMVVNNFSIGIIGFILMCLSYLVVGPIIQSANQFVTSA
                     IEALVSTGFLPILSLINEPAKVLFLNNVIDQGVYYPLGMQQTLEAGRSIYFMVASNPG
                     PGLGLLLAYSLFGKKAAKQTAPGAIIIHFFGGIHEIYFPYVLMKPLTILSMIAGGMTG
                     IITFELFDAGLVAGPSPGSIFSYLALTPRGNFLGVMAGVVTATLVSFFVTSVILKADK
                     STDEDRNFELSVQKSKTMKSEGRKLLKEENAREICTEKKEMNKISFACDAGMGSSAMG
                     ATIFKKKLQKAGITDVEIKNYRIEEVPEDSDLVIVHQDLEERTRKAHPNMKIVTLKNY
                     LQDPVINGLAEEIIGLKTED"
     gene            complement(6353..6784)
                     /locus_tag="ACHALG_001469"
     CDS             complement(6353..6784)
                     /locus_tag="ACHALG_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFN2586885.1"
                     /translation="MLKEQLSGNTMFMDAIDSWQEAIRVSARPLIGKGIIEESYVQAM
                     IQNVLDNGNYIILLPLVAMPHARPEFGSKGVGMSFLCLDKPVMFPSEEPVKFFFTFSS
                     DSPKGHLDLMANLGELLSDEKMYQKLFDVKTEKALLDLIKK"
     gene            complement(6796..7935)
                     /locus_tag="ACHALG_001470"
     CDS             complement(6796..7935)
                     /locus_tag="ACHALG_001470"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFN2586886.1"
                     /translation="MKAVHFGAGNIGRGLIGQILHQNGFDLCFIDTNPELIHQLNQDK
                     GYTIEIIDEMNRSDFIDHVVALNSLSELEEVITATKNADILTTSVGAINLVKIAPIIA
                     QALSKRFEQKHSINIVANENVINASDVLKKEIYSLLSEKEQENFDEYAYFVNTAIDRQ
                     SLGKVLGGKMISVVEPYYEWIMDRQQLDPTTSFDMKNVNLVDDMEPYIERKLYIVNAA
                     HAAVAYLGALKGYKTIQEAIQNMEIFNLVKQFLMENIEYFVQKYHFSREELTSFVEKT
                     LNRHGNQKLSDEITRVGSSPIRKLGPNDRLIAPIVKLDSYHLPYDAGIKVIAAGYHYR
                     NLADPEAEQLRRMIVNDGLKATIKKISRLEGKLLNEVVAAYESQI"
     gene            complement(7943..8500)
                     /gene="hxlB"
                     /locus_tag="ACHALG_001471"
     CDS             complement(7943..8500)
                     /gene="hxlB"
                     /locus_tag="ACHALG_001471"
                     /EC_number="5.3.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0043800 - hexulose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-3-hexuloisomerase"
                     /protein_id="HFN2586887.1"
                     /translation="MSYKAAKQNIVREITETLDRISSKDVEQLIKKIEKAERVFFVGV
                     GRVLLALEGMAKRLGHLGIQTVVVGQITEPAITDKDLLIVGSGSGESAFPLTIAKKAK
                     EFGSQVAHIGSNPDSTMKEYSDLFIRIPVQTKLALPEEISSIQPMTSLFEQSVLLLGD
                     SIALVMIEEKAIDMSKLWQYHANLE"
     gene            complement(8540..9244)
                     /locus_tag="ACHALG_001472"
     CDS             complement(8540..9244)
                     /locus_tag="ACHALG_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFN2586888.1"
                     /translation="MEKKIILSPSLMCGDLANLEGSIQELESIGMDSLHIDVIDGSFS
                     PSMPLGIETIKRIREITSMNFDVHIMAKDNDNEFFIGEMLKIGVESITFHYETSLHID
                     RYIQLIKNSGTKVGIALNPATPLNVLQYILPEIDRICLMLINPGFATNKSEKQVSYAL
                     KKITDLCQLVNEKELAVDIQVDGRVSLETIPGLLDAGATNLVLGSTSLFLKGNTLAEN
                     QKLLYQAMKQSVRKNQ"
     gene            9430..10179
                     /locus_tag="ACHALG_001473"
     CDS             9430..10179
                     /locus_tag="ACHALG_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFN2586889.1"
                     /translation="MLKLERHQKILDLLNAEQKVIASDLSQRFNVSEDTIRRDLKDLD
                     SQGLLKRVHSGALRVGPPITNFNYRETVASEQKTALAHKALPFLKEESVIIIDGSTTN
                     LALAKEIPQDFRATIITNSPPIAIALAQHPNLEIINIGGTFYKQSMINIGVQAYKQLQ
                     NIRADLYIMGIYNIDLDVGTSVPTMQESDIKREMIRISTEVLGMITNDKFGTISNHIV
                     GPIEDLTYLISEEIPEKVRKEYNKQKIVLID"
     gene            complement(10363..10608)
                     /locus_tag="ACHALG_001474"
     CDS             complement(10363..10608)
                     /locus_tag="ACHALG_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2586890.1"
                     /translation="MSKSKKNITIIGLGLTVYLFTEFVYRHVNLFAEPLFVLYESNSS
                     IVSELANLMIALLFTPALRVITILVCLVLLFFVNKKK"
     gene            10928..11689
                     /locus_tag="ACHALG_001475"
     CDS             10928..11689
                     /locus_tag="ACHALG_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFN2586891.1"
                     /translation="MHTPEEIMELSIHLGTKKIHKPILAKLILGFIGGAMISLGYLAY
                     VRVSASIPESMASLTSLVGASVFPIGLLVILLGGGELITGNMMAVAIAFMDKKVTFSQ
                     LVKNWSVITFANIIGAIFVAYFFGHFVGLTHSGVYLDQVIHIADHKIAVSWWQALGSG
                     IGCNWFVGLALWLSYGAKDAAGKVLTIWFPVMIFVAIGFQHSVANCFVIPAAIFEGQA
                     TWLEFLSNFIPVYIGNIIGGAIFVSGFYYVSYKHH"
     gene            11917..12111
                     /locus_tag="ACHALG_001476"
     CDS             11917..12111
                     /locus_tag="ACHALG_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2586892.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            12380..12679
                     /locus_tag="ACHALG_001477"
     CDS             12380..12679
                     /locus_tag="ACHALG_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2586893.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(12776..13210)
                     /locus_tag="ACHALG_001478"
     CDS             complement(12776..13210)
                     /locus_tag="ACHALG_001478"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN2586894.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(13442..14152)
                     /locus_tag="ACHALG_001479"
     CDS             complement(13442..14152)
                     /locus_tag="ACHALG_001479"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFN2586895.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(14142..15002)
                     /locus_tag="ACHALG_001480"
     CDS             complement(14142..15002)
                     /locus_tag="ACHALG_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFN2586896.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(15007..15645)
                     /locus_tag="ACHALG_001481"
     CDS             complement(15007..15645)
                     /locus_tag="ACHALG_001481"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFN2586897.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(15660..15959)
                     /locus_tag="ACHALG_001482"
     CDS             complement(15660..15959)
                     /locus_tag="ACHALG_001482"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFN2586898.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKEVTVIGIKNVMSAKEISEKFAETKWYQKNK"
     gene            complement(16002..17486)
                     /locus_tag="ACHALG_001483"
     CDS             complement(16002..17486)
                     /locus_tag="ACHALG_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFN2586899.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQVLIFMGLILGCYWAVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPEGYFLIVEDYDTYAEKQRV"
     gene            complement(17506..17967)
                     /locus_tag="ACHALG_001484"
     CDS             complement(17506..17967)
                     /locus_tag="ACHALG_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFN2586900.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGEDEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(17985..19052)
                     /gene="ulaG"
                     /locus_tag="ACHALG_001485"
     CDS             complement(17985..19052)
                     /gene="ulaG"
                     /locus_tag="ACHALG_001485"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFN2586901.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADSKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            19270..20040
                     /locus_tag="ACHALG_001486"
     CDS             19270..20040
                     /locus_tag="ACHALG_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFN2586902.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(20313..21140)
                     /locus_tag="ACHALG_001487"
     CDS             complement(20313..21140)
                     /locus_tag="ACHALG_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN2586903.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            21707..22942
                     /locus_tag="ACHALG_001488"
     CDS             21707..22942
                     /locus_tag="ACHALG_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFN2586904.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(23941..24201)
                     /locus_tag="ACHALG_001489"
     CDS             complement(23941..24201)
                     /locus_tag="ACHALG_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFN2586905.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(24228..25529)
                     /locus_tag="ACHALG_001490"
     CDS             complement(24228..25529)
                     /locus_tag="ACHALG_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFN2586906.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEKAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNATLLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPMIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(25821..26279)
                     /locus_tag="ACHALG_001491"
     CDS             complement(25821..26279)
                     /locus_tag="ACHALG_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFN2586907.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(26292..27122)
                     /locus_tag="ACHALG_001492"
     CDS             complement(26292..27122)
                     /locus_tag="ACHALG_001492"
                     /inference="COORDINATES: protein motif:HMM:NF023401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HFN2586908.1"
                     /translation="MINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYD
                     KELQREIMPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYY
                     LYPDMVVEHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDI
                     AISLLNDDRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVETITLRKPISDFHKGL
                     MEAQVAAEKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK
                     "
     gene            complement(27135..27622)
                     /locus_tag="ACHALG_001493"
                     /pseudo
     CDS             complement(27135..27622)
                     /locus_tag="ACHALG_001493"
                     /inference="COORDINATES: protein motif:HMM:NF014148.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
     gene            complement(27633..29075)
                     /locus_tag="ACHALG_001494"
     CDS             complement(27633..29075)
                     /locus_tag="ACHALG_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFN2586909.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAIFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSGFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(29062..29826)
                     /locus_tag="ACHALG_001495"
     CDS             complement(29062..29826)
                     /locus_tag="ACHALG_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HFN2586910.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(29930..30805)
                     /locus_tag="ACHALG_001496"
     CDS             complement(29930..30805)
                     /locus_tag="ACHALG_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFN2586911.1"
                     /translation="MKESASCFVLSYSGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAIEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(31060..31144)
                     /locus_tag="ACHALG_001497"
     tRNA            complement(31060..31144)
                     /locus_tag="ACHALG_001497"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31108..31110),aa:Leu,seq:caa)
     gene            complement(31162..31233)
                     /locus_tag="ACHALG_001498"
     tRNA            complement(31162..31233)
                     /locus_tag="ACHALG_001498"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31199..31201),aa:Gln,seq:ttg)
     gene            complement(31243..31315)
                     /locus_tag="ACHALG_001499"
     tRNA            complement(31243..31315)
                     /locus_tag="ACHALG_001499"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31280..31282),aa:His,seq:gtg)
     gene            complement(31377..31461)
                     /locus_tag="ACHALG_001500"
     tRNA            complement(31377..31461)
                     /locus_tag="ACHALG_001500"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31425..31427),aa:Leu,seq:caa)
     gene            complement(31482..31552)
                     /locus_tag="ACHALG_001501"
     tRNA            complement(31482..31552)
                     /locus_tag="ACHALG_001501"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31518..31520),aa:Cys,seq:gca)
     gene            complement(31598..31669)
                     /locus_tag="ACHALG_001502"
     tRNA            complement(31598..31669)
                     /locus_tag="ACHALG_001502"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31635..31637),aa:Gln,seq:ttg)
     gene            complement(31681..31753)
                     /locus_tag="ACHALG_001503"
     tRNA            complement(31681..31753)
                     /locus_tag="ACHALG_001503"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31718..31720),aa:His,seq:gtg)
     gene            complement(31756..31829)
                     /locus_tag="ACHALG_001504"
     tRNA            complement(31756..31829)
                     /locus_tag="ACHALG_001504"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31795..31797),aa:Trp,seq:cca)
     gene            complement(31836..31916)
                     /locus_tag="ACHALG_001505"
     tRNA            complement(31836..31916)
                     /locus_tag="ACHALG_001505"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31880..31882),aa:Tyr,seq:gta)
     gene            complement(31918..31993)
                     /locus_tag="ACHALG_001506"
     tRNA            complement(31918..31993)
                     /locus_tag="ACHALG_001506"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31958..31960),aa:Phe,seq:gaa)
     gene            complement(32029..32101)
                     /locus_tag="ACHALG_001507"
     tRNA            complement(32029..32101)
                     /locus_tag="ACHALG_001507"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32066..32068),aa:Asp,seq:gtc)
     gene            complement(32111..32183)
                     /locus_tag="ACHALG_001508"
     tRNA            complement(32111..32183)
                     /locus_tag="ACHALG_001508"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32148..32150),aa:Val,seq:tac)
     gene            complement(32199..32270)
                     /locus_tag="ACHALG_001509"
     tRNA            complement(32199..32270)
                     /locus_tag="ACHALG_001509"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32235..32237),aa:Glu,seq:ttc)
     gene            complement(32285..32373)
                     /locus_tag="ACHALG_001510"
     tRNA            complement(32285..32373)
                     /locus_tag="ACHALG_001510"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32337..32339),aa:Ser,seq:gga)
BASE COUNT        10305 a         6503 c         4939 g        10663 t
ORIGIN      
        1 taaaatctta atcggtttaa catttgttaa aaataaatca taataatcta acacataata
       61 ataatccaaa aataattgtg cccagttttc taatttcttt gatacatcca aatccgcatt
      121 tgcttgtaat tcttttttga gtttttccat tatgtgataa gttcgaggtt cttcttcttt
      181 catttcttcg atatacgaac gattaaaaaa aagatctgga ttaccataaa atagataact
      241 tctttcatga aatgcaataa aattgaatat gagttctgga tcctttatat ctaaagaaaa
      301 acggaacatg ctttgtaaaa ctttctccaa attctcaact gctcgataag aacaagtatt
      361 ttgtcgctga tgtgcatatg catgactatt ttcatatatt gcatttccat caattactcc
      421 aaaagaataa attattcgat aaaggaaaca cagttcatcg ctgttaaaag aaagattgat
      481 ttgttttgac cacttcttca aaagattaaa atgcggatca tctgagataa ctgatttata
      541 ttcattatct actcgtactt ttctaaatga acttctttct atattaatgg ctaaccaaaa
      601 agaaaataca cgtttttgaa aaatagttaa cgtaatatca taaatcccct caaaagcaac
      661 tatatacttc tctatttcct tttgttcgat atgaaatggc cacttatcac tactttcacg
      721 tgtagaattc caatagaaat aataaaaaaa gctacgaatg tttgcctctg atgcattcaa
      781 ttctaactga aatggatgaa atgttatatg ataacgccta aaatatggaa ttaagagctt
      841 cgcatatctg aaaacagtag atacactagt aaattgctct ttagcaaaat cttttagaac
      901 acttctagga tatttataca taaaatcaat caaatgataa ttataagaag ctttataaaa
      961 atgaagataa atattataaa taggaaaatc tttctctttt tttatgaaaa atcgtttatt
     1021 atcttcattt ttcagaagga tattaccatt atattgctta aataaatcct ctagttcttc
     1081 tatatagtgt aagactgact tctccgtaac attaagtaaa tttctaagtt cttccattgc
     1141 acaccaattg tctttttcta acaaataatc taataaaact acttttttga aagatgtttt
     1201 agttaaaaaa gtttgcatca tttcacctca ataagttact tttgtttatt ttaatcaaag
     1261 aataaacttc tctcgttcga ctataactga tcgaacaaaa gaagcctttt aataaatata
     1321 atctcactta catacatagg ctagttattt gcttctagct tatgataagt atataaacgg
     1381 atatcgattt cataattgtt ttatcttaaa catcatagct gtaattgttt ttatttttac
     1441 atgaagaatt attataatct agtttaattt ttttctgaaa tatagaataa aaataaaaac
     1501 atcttttcat tttgagttat taatggagat gtaaatgatt aacaatatgt aatttaattt
     1561 tttattcctg cattaagata atttgattgg taaataaaaa caaacgtaaa ttcgttattt
     1621 taattcctat ttattgtgaa catatccaat ttttatcaca tagatatttg tataaacatt
     1681 ttatgaatgc gcttgtattt aatttagaaa gtgttactat ctatttaggc aaatttttgc
     1741 taatattttg ttttttcatt taccatctgt agaaatcatt taatgatttc tatcttctcc
     1801 ttccaacttt tctgatgatg catcatgaat taacagtgca ttatccatct tttccatgtt
     1861 aaaagaacta agaatcaaga taacgtcttg attcttagtt cttttattcc ttctagaaaa
     1921 taattgttag caatatagtt tatatttgta aatactaaat cagtagcttc tagttgcttt
     1981 cctgcttaat agttcatatt actcaatgta aaaaataatt gattgtggac attgctcatc
     2041 cactttgtat ccacaacttt ctttataata ggtatgataa tcactttcta ccatattaat
     2101 taactctttt cccccgcaaa attcttcaat gtctaaatta ataaattcct ctaaaaggga
     2161 aagcttagag tcaatcgaaa gcattttagg taacatagtc caaaagtcat tatcatcgtt
     2221 tttacgtagt gtataatatg tttgcttttc tagatttaat acataattat atgcatcata
     2281 atctcccttt ttttcaaaaa aatttttcat atttaaaaat ctactcacaa attttttctt
     2341 gaaactattc attttctcac ctctatttat tctttttctt gtccatagca taacttttga
     2401 atagagtaac taaattcaaa agctattata gtataaaaat acactttttg ttgttcaaat
     2461 ttttcatcta attgtgcagt aattttgaac tctatatttt ttaatattta atgcttaagc
     2521 aatcatttat aaaaaaagac tggacgataa ttaaaataac aaaaaaggct gtgacacgtc
     2581 ttttgtcaca gtcccttcta gcattatatt actattattt attcactgca tcttcttaag
     2641 agtaaaacat acttgatcgt ttgtttcttt actaacagcc ttctttactt ggttcctacg
     2701 atccttaatt ttatctaagc tgattgctgc ttgtaaccat aaaacttcga tcagaacata
     2761 catcctagta ttgtcgtctc gctcatgttc tgtataacaa agaggataaa tccaaagcga
     2821 tttctttcca agaagctctc agttaacgac ctcgcccatc tcttttttac cacgaatatt
     2881 aaaaagctcc tcctcttaaa caccgtttat catatgaagt attttttatt taaattgatt
     2941 cacctattag gaaaaagtaa ggttaacaca cgtaatcact accttattga ataactgaag
     3001 caatatactt tgcataagct gtacgtccac tttcattcaa atgggtttca tcatcataaa
     3061 aaaattcagg atgatttttg ctctcactat accaatcgat catcgttata ttagaatact
     3121 gatttggcaa tgtagctaat gtggcattta tcgtatcttg ccagccagtt ggtgctttta
     3181 tatttaccca gtatactttt ttatctttca gcaaagacat tgtttgttct atagcttcat
     3241 ctgttatcac accattggtt cctaacccga tcactatttg ttggtaagaa actaaatctt
     3301 tatgctcctt taagatttct ggtaacgcag tgatttgtct tgattctttt gcatctacca
     3361 atgcttcttt tttaaatgtc tcctttagtt cctgatacgc ccccagcata acagaatcac
     3421 cgataaataa tattttcttc tcttgtgaca acagattttc tcctgcattt gctttactag
     3481 agttttcttt taataaactt tgcaactgat tttggtcagc ggtcagtcga ctggcaggag
     3541 cacgtaatac cagtacagaa actccgatta tcattagtgc catagctgtc caaattttgg
     3601 gtgatctcat cttttgcact ccttttctaa agcaagtaat catgagtaat gtacataaaa
     3661 acacagtgaa tagacctgcg atatccaaat atttccaatg gtgatgtttg aatagatttg
     3721 tcagagcaat caatactggg aaatgcacca aataaagttc ataactgatt tttcctacaa
     3781 aagaaagttt tgtactagcg attttggtct tttttgcaat gttcatatca tcgacactgg
     3841 ctaacaatag agcaaaacaa atactgatca gtagttcatt tagtgtataa ggaaaaatcg
     3901 ggaacagaat cagacaaaga ctagctagta ataaaagcca gattttacta aaagtgtttt
     3961 gttcaaagaa acggccaata gttattcgat taaaataccc ccaaactcct aataaaaatg
     4021 gaaaaagttt agctatcgta tgataatatg ccttagttga aggtaaaaac aaactaaaaa
     4081 gaatagaaac aaaaatcaat gtatagatta agtaaaacac ttgttttctt ctcatacgct
     4141 taatgacaat aaacaaaaat ggccatatca aatagaattg tagttcaact gaaagcgacc
     4201 aataatgttt aaataaagaa gcattaaagt aagagtcaaa ataagatact ccttgattca
     4261 gctgccacca attgttatag ccaaaaatca aactgggtag ctcactacga atgtttccta
     4321 tcaatcgaat gttgctaatc cctagtatga aaatgaatac gaccaaagaa aacagtaaaa
     4381 atggataaat acgtttgata cgcttatagt aaaaatgtaa aaaactgaat tgtctgtttt
     4441 tttcatctct tatcgtattt ttagccatta aaaaaccaga aaccacaaaa aagatcgtta
     4501 ctccttgcaa tccgcctggc agcaaactat ttggcaaatg ataaaatatc actgctaaaa
     4561 cagcaatgcc tctaaaacaa tcgatttcct ttgaatatat ttttttcatg tatcatcaac
     4621 ctccttctct tcatgcatgt tttaatcaaa catattcttc cgtatcgatt ttttcatttt
     4681 tctattcgtt tcaccttgtt ttgtaaatta acagttaaaa gtatctaaaa tgtatctttg
     4741 ccccatcagt tctcgttgat cacaaaaaat atgaacataa aatatccgat taattatttg
     4801 tgcgacaaaa aaaggttgcg acacaagcca tgtcacaacc tttttatatt aggcaaaata
     4861 attttaataa aggttaatca aaaattccca taaattagtc ttctgttttt aatccaatga
     4921 tttcttctgc taatccattg attactggat cttgcaaata attctttaat gtcacaattt
     4981 tcatgttagg atgtgctttt ctagtccgtt cctccaaatc ttgatgtacg atcaccaaat
     5041 cactatcttc tggtacctct tcaattcgat aattttttat ctcaacatca gtaatacctg
     5101 ctttttgcaa ttttttcttg aatattgttg cgcccattgc gctgcttccc attcctgcat
     5161 cacaagcaaa ggaaatttta ttcatctctt ttttctcggt acatatttct ctagcatttt
     5221 cttcttttaa taattttctt ccttctgatt tcatcgtttt cgatttttga actgatagtt
     5281 caaaatttct gtcttcatct gttgatttat ctgcttttaa aattactgat gttacaaaaa
     5341 aggaaaccag tgttgctgtg accactcccg ccatgacacc caaaaaattt ccccgaggcg
     5401 ttagagctaa ataagaaaaa atagaacctg gacttggacc tgctaccagg cccgcatcaa
     5461 ataattcaaa agtgatgatt cctgtcattc caccagcaat catagataat atagtgagtg
     5521 gtttcattaa tacatatgga aaataaatct catgtattcc gccgaaaaaa tggatgatga
     5581 ttgcacctgg tgctgtctgt tttgctgcct ttttaccaaa taaagaataa gctaacaata
     5641 gtcctaagcc agggccagga tttgatgcta ccatgaaata aattgatctg cctgcctcta
     5701 atgtttgttg catacctaat ggataataaa caccttggtc aataacatta tttaaaaata
     5761 agacttttgc cggttcgttg attaaggaca gtatcggtaa aaaaccagtt gaaactaatg
     5821 cttcaattgc actcgttaca aattgattag ctgattggat tattggtcca actaccagat
     5881 aagaaaggca catcagtatg aaaccaataa ttcctataga aaaattattg acgaccattt
     5941 caaaaccagc aggaactttc ccatctaaaa aatcatctgt ttttttcatc atccacccac
     6001 caagtggccc cataatcatt gctcccatga acattggtat gtcagcacca ataatcaaac
     6061 ctattgttcc gattgtacct aaaacagctc cacgtgtacc accaaccatc cgaccaccag
     6121 tataggcaag tagtaacggc aatagatagc tgactgtcgg acttacgagt tcattcagag
     6181 attcatttgg gaaccaccca gttggaatga ataatgcagt aacaattccc catgcaataa
     6241 atgcgcctat atttggcaat accatatttg ttaagaaacc gccaatcgct tgaactttag
     6301 ctcgcatgga gactcgtttt ttttgtttgc tacctccatt ttcttctctc ctttacttct
     6361 ttatcaaatc taacaatgct ttttctgtct tcacatcgaa taatttttga tacatttttt
     6421 catcactcag caattctcca agattggcca ttaagtctaa atgtcccttt ggcgaatcag
     6481 aagaaaaggt aaagaagaat tttactggtt cttcacttgg gaacatgact ggcttatcta
     6541 aacataaaaa gctcatcccg acccctttgc tcccaaattc tggacgcgca tgaggcattg
     6601 ctactagtgg cagtaatata atataatttc cgttatccaa cacattttgt atcatcgctt
     6661 gtacatagct ttcctctatt attccctttc ctatcaaagg acgcgcactt actcggattg
     6721 cctcttgcca tgaatcgatc gcatccataa acattgtatt tcctgacaat tgttctttta
     6781 gcatattctc ctcctctaaa tctgggactc ataagcagcg acaacttcat tcagcagctt
     6841 accttccaaa cgagaaatct ttttaatcgt tgctttcaac ccatcattta caatcattcg
     6901 acgtagttgt tcagcttctg gatcagcaag attccgataa tgatatcccg cagcaattac
     6961 ttttattcct gcatcgtaag gtaaatgata tgaatctagt tttactatgg gagcaattaa
     7021 acgatcatta gggcctaatt ttcgaatagg ggaacttcct acacgagtaa tttcatcgga
     7081 gagtttctga ttcccatgac ggtttaatgt tttttccaca aaagatgtta attcttctct
     7141 agaaaaatga tatttttgta caaagtactc tatattctcc attaaaaatt gcttgactag
     7201 attgaaaatc tccatatttt ggattgcttc ttgaatcgtc ttatatcctt tcagtgcgcc
     7261 taaataagct actgccgcat gagcagcatt aacgatataa agctttcgct caatgtaagg
     7321 ctccatatca tcaaccaaat ttacattttt catatcaaaa gatgtagttg gatctaactg
     7381 ctgtcgatcc attatccatt cataataagg ttcgacaaca gagatcattt ttcctccaag
     7441 aacttttcct aaagattgtc gatcgattgc cgtattcaca aaatatgcat attcatcaaa
     7501 attctcttgt tctttttctg acagcaaaga ataaatttct tttttcaaca catcacttgc
     7561 atttatcaca ttttcattag ctacgatatt gatgctatgc ttttgttcaa atcgcttact
     7621 taaggcttgc gcaatgatcg gtgcaatttt tactaaattt attgcgccaa cagatgttgt
     7681 taagatatct gcattttttg ttgctgtaat cacctcttct agctcggata aactattcag
     7741 tgccacaaca tgatcaatga aatcactcct gttcatttca tcaataattt cgatagtata
     7801 acccttatct tgatttaatt gatggatcag ttcaggattc gtatcaataa aacatagatc
     7861 gaatccattc tgatgtagaa tttgtccaat aagccctctg ccgatgttcc ctgctccaaa
     7921 atgtaccgct ttcatttttc acctactcta agttagcatg gtattgccac aactttgaca
     7981 tatcaattgc tttttcttct atcatcacta aagcaatact atctcctaac aaaagaacac
     8041 tttgttcaaa caggctagtc attggctgta tagaagaaat ttcctctggt aacgctaatt
     8101 ttgtttgtac gggtatacga ataaaaagat ctgaatattc tttcattgtg ctgtctgggt
     8161 ttgatcctat atgggcaact tgggacccaa attcctttgc tttttttgca atagttagtg
     8221 ggaaagcact ttccccacta ccagaaccaa caatcaataa atctttatct gtaattgccg
     8281 gttcagtgat ttgtcctacg actactgtct gaattcctag atgccctaag cgttttgcca
     8341 ttccttctag cgctaacagt actctgccaa cccccacgaa aaaaactcgt tccgcttttt
     8401 caattttctt tatcaattgt tccacatctt ttgaagaaat acggtctaat gtttcagtaa
     8461 tttctcttac aatattttgt ttcgccgcct tataactcaa ctatttcccc tccatttcta
     8521 ttattcagga ccactacatc tattgatttt tcctaactga ttgcttcatc gcttgataaa
     8581 gtagtttttg attttctgct aatgtatttc ctttaagaaa caaacttgta cttcctaata
     8641 ccagatttgt tgcgcctgca tctaataatc ccgggatcgt ttctaacgat acccgtccat
     8701 ccacttggat atctactgct aactctttct catttactaa ctggcaaaga tctgtaattt
     8761 tttttagcgc gtaagaaact tgtttttctg acttgtttgt tgcaaaacct ggattaatca
     8821 gcatcaaaca aatcctatca atctcgggta aaatatattg taatacattt aacggagttg
     8881 ctggattcaa agctatccct acttttgttc cagaattctt gatgagctgg atatatcgat
     8941 caatatggag gcttgtttca taatgaaaag taatgctctc tacgccaatt ttcaacattt
     9001 ctccaataaa aaattcgtta tcgttatcct tagccatgat gtgtacatca aaattcattg
     9061 aagtgatttc tctaattctt ttgattgttt cgatccctaa aggcatactg ggactaaaag
     9121 aaccgtcaat cacgtcaata tgcaaggaat ccatcccaat actttctagt tcttggatgc
     9181 ttccttccaa atttgctaga tccccacaca taagtgaagg cgacaaaata atttttttct
     9241 ccatacattc cctcctttaa ttaccccctc attgtaaccg cttaacaaat actagtcaac
     9301 ataaaactgc ataaaaatat tttgtttttg cttgtttatg cctttttagt gtattaaaaa
     9361 cggaaattat tgacataaaa acgcataaat gacatgataa acaagaataa ataagaagga
     9421 gcacgcaaca tgttgaaatt agaacgtcat caaaaaattc tagatttatt aaacgcagaa
     9481 caaaaagtta tcgctagtga tttaagtcaa cgtttcaatg tatccgaaga cacgattcga
     9541 cgagatttga aagatttgga tagccaaggt ctcttaaaaa gagtacacag cggcgcttta
     9601 cgtgttggtc cgccaattac caattttaac taccgagaaa ctgtagctag cgaacaaaaa
     9661 actgcattag ctcataaagc cctccctttt ttaaaagaag aatctgtcat catcattgat
     9721 ggaagtacaa ctaacttggc tttagcaaaa gaaatcccac aagattttcg tgcaacaatc
     9781 attaccaata gcccgccgat tgccatagca ttagcacagc atcctaattt ggaaattatc
     9841 aatattggcg gtacttttta taaacaatct atgatcaata ttggggtaca agcttataag
     9901 caactacaaa acattcgagc cgacctttat attatgggta tttataacat agatctagat
     9961 gttggaacga gcgtgccaac tatgcaagaa tccgacatta aacgtgagat gatacgtatt
    10021 tctactgaag tacttgggat gataactaat gataaattcg gcaccatcag taatcatatc
    10081 gtcggtccaa ttgaagacct tacctatctt atttcagaag aaattcctga aaaggttcgt
    10141 aaagaataca ataaacaaaa aatcgttttg attgattaag cgaaaactat tggtaactac
    10201 taaagcaaca gatataaaaa atctaaaaaa gaaacacgca ataaagtttt gaaataaact
    10261 ttattgcgtg tttccttttt tgtttattct cacttaccta gcaccacgta taacatacta
    10321 tagcaatcat catcccactt ctttgtcttt tgaaatacta cgttatttct ttttattaac
    10381 aaaaaacaat agcactaaac agaccaaaat agttatgact ctcaacgcgg gagtaaacag
    10441 caatgctatc attaaattag caagttcact gacgattgag gaatttgatt cgtataatac
    10501 aaataaaggt tctgcaaata aattcacatg tctataaaca aattctgtaa ataaatatac
    10561 ggttaagcct aaaccaatta tggtgatatt ttttttagat tttgacattt tttctctcct
    10621 ataataattt tttcagttat tcatttcctt ttttataaaa aatgtagatt gttttgaagt
    10681 aataatatgc tgtagtttca agaaaattta caggaaattt gataactgat attatttact
    10741 ttttgaccac catatacatg acttttattt ttgtttcttt tttagaatca aatgtgatta
    10801 aatagaaaat tttacaataa tatgcacata taaataataa gatgatataa tcaacaaaga
    10861 aaatgtgatt tttttcacta tattttatca catgaaaatt cattaagaaa gtaggaatcg
    10921 aagtattatg cacacaccag aagaaattat ggaactcagt attcatcttg gaacaaaaaa
    10981 aatacataaa cctatcttag ctaaactgat tttaggtttt atcggaggag ccatgatttc
    11041 tctaggttat ttggcatacg tacgagtctc tgcttctatc ccagaatcta tggctagctt
    11101 gactagttta gttggtgcct cggtttttcc tatcggcctg ctggtcatcc ttcttggcgg
    11161 tggtgaattg atcaccggaa atatgatggc tgtggcgatt gcctttatgg ataaaaaagt
    11221 cacattctca cagctggtta agaattggtc agtgattact tttgcaaata ttattggtgc
    11281 aattttcgtt gcctatttct ttggacactt tgttggttta actcattcag gtgtctatct
    11341 cgaccaagtg attcatattg ctgatcataa aatcgctgtt tcttggtggc aagcattagg
    11401 ctccggaata ggttgtaatt ggtttgtcgg tttagcgtta tggttatcat atggagcaaa
    11461 agatgcggcc ggcaaagtac taacgatttg gtttccagtc atgatttttg tggcaatcgg
    11521 tttccagcat agtgtggcca actgttttgt gatacctgct gccatatttg aaggtcaagc
    11581 gacttggtta gaatttctct cgaattttat ccccgtttat attggaaata tcatcggcgg
    11641 agctattttt gtttcaggtt tttattacgt tagttataag catcattaag attgaaggta
    11701 atgttcattc gagcattacc ttcaatctct ttatatacta tttatatttt aagaacaata
    11761 gccttttatt atatatatta ttcaaattag ttatatcgag tgaaaaaaac tttcaaattg
    11821 attcttttta tttatcataa agatatagat acataatcat tttcacttac atatatttga
    11881 atattaacta aaaacagcaa gaaagaagag gatcaaatgt attatgtaga agtaaaaaca
    11941 aaaggcgtca aaaacaaaca atatgtgaaa ggtatcagta acgaatatcc tttacttggt
    12001 tcatggaaag aagctgctcc tttttccaaa ccatgtgcca taaaaataaa gaacgagcta
    12061 gaaaaagaac tgacttgtgg caaagctgtt gtagatatca tcgaaaagta aatccaatgt
    12121 atagaaagga agatcacaca tgaaaagcga cgtacaaatt gctaagatta tagaattaga
    12181 agaagaagac gcagtgttga caattgaaaa aaaataaatg tttgtatacc atatgattat
    12241 ttcaatatgt atcctggaat tggagatctc gtagagctat atcaagatga atcaaatata
    12301 cttgtcattc atcatgaaat atagagactt ataaaattgt ttatttttat aatgtatcag
    12361 tatagaaagg aaataaaaca tggattatac tgttgaattt gacagtgaac tacttaagca
    12421 atttccacaa gaagaacatt tcatgtataa agaagcaatt tcccacaaag tgacagagta
    12481 tttggagcat aatttttttc gtataaagcc agtgcgtact cgttcggcaa aaggaatata
    12541 cgagatgaaa gtcaaaatta ataaaaaaaa ttatcgcata gcatttgctt taaaaaaaaa
    12601 tcatgtgttc attttttata ttagttcgaa tttgcaaaaa attgtatttg ataaagaggt
    12661 gagtaaaaaa ataaaatagc tgagcattgg aggatagatt gagtgatact aagtctatcc
    12721 tcttgttttt ctaaagaact aagctagaat tttttctttt gtacgttatt atatgttatt
    12781 tgtcattacc aaaaagcacc ttttttcttc tagtatctat gacataagaa ataccaccca
    12841 tttcctttac ttgaacaaat cctactgatt cataaaattt ttttgttttc tcagtatcat
    12901 ctgtcaataa ttcgatttgg actggtccat aactagtgat cttctccaaa gcttcattca
    12961 aaagcacttt gcctattcct tggcgctgat acctcggatg aatgaggata tcttgaataa
    13021 atgcgatcgt cgcaaaatca gtgatccaac gaacgattcc gataatcttc ccttccccat
    13081 tacgctttat cagagattca gattgttcaa atgctctagc taaccttgca gtgtcttttg
    13141 tgtaattcgt ccaaccaacg ctcttatata attcagaaat ctcatcgatc ataagggaat
    13201 caatattcat ttttttcttc ctttcttata caattctccc tctaataata cagttctttg
    13261 actatttaat ctatcacata taactctcct gtaaagattc tctcgattcc ttaaaatctc
    13321 tctttccttt tagaatgcat cctttttgat aaataaaaag gttgtgataa attttcttca
    13381 caacctctgt ttctctctat caagtgttat cttttaattt tctaccctta ccctagcatc
    13441 tctattgccc gtagtatgca tactttccat gtttgcgaaa ataatgttta tctagtaaat
    13501 attgcgggat ttcacatata ttggggttga tcagctcgtt ttctttggcc atcagacaaa
    13561 tttcatctaa gatcaaactg ttttctactg ctttcatagg tgaatccccc catgtgaacg
    13621 gtccatgtcc atagacaagt actccaggga cttcgtttgg atctaatttt ctttccttaa
    13681 acgtctcgac aatcacattc cctgtatgaa cttcgtaagc ttctcttact tcttctttcg
    13741 ttaattggcg tgtacaagga accttaccat aaaaagcatc agcatgagtc gtgccataag
    13801 ctggcaaatc gcgtcctgct tgcgcccaca tgacggagtg cgtggaatga gtatgagtaa
    13861 tagcagttat atcttcaaaa gtttgataca aaacaacatg tgtggggaga tctgaagatg
    13921 gttttaagct atcttcttct attatatttc ccgacaaatc tgttaccacc atttgatctg
    13981 cttgcatgca ttcatatctg acaccgcttg gcttaatgac gatgactcct agctcacggt
    14041 ccacttcact cacattgccc caagtcaatt tgactaatcc gtatcgcggc aatgccagat
    14101 ttgcttcaag gacacgttgc ttcattgctg caattttttc ttcacaagac atcatagcca
    14161 gcctccttca atttagaact taagtagtct tttgcctgct ggatttctct tcgaaagtct
    14221 tggctttttt cactccacat ttcaatcaga aatgttccat cataatctag acgtttcaaa
    14281 tgtcttaata atccaagaaa atcaacacaa ccgctaccaa aaggaacatc acgaaactgg
    14341 cctgaatatt cttttgttac agggtaagta tcttttaagt ggatagcagt gatataatcg
    14401 atccctttct caagttctcg agccggatta ttttccggcc atgcggacaa attgcctagg
    14461 tctggatata cttgtagaaa aggggaatga atctgttctt tgatttctaa aaattttgaa
    14521 atggagttca taaatggatc atccatgatt tccacggaaa gaatgatgcc atatttagaa
    14581 gcttccttta cgctttcttt tattccttcg ataaagtctt ctctggaatt cattgatttc
    14641 tcttcataat aaacatcgta tccagctatt tggattactt tgacgcttag attatgcgcc
    14701 aaatggattg cttttttcat tagttgtttc gctgcttttt tcttttctgg atcagcagaa
    14761 ccaaacggaa atcgccgatg cgcacttaaa caaatcgaat ggatacgtac accggagctg
    14821 aacatctctt ctctcagctc agctatcttt tcttcagacc aatctaatcg tgctaaccgc
    14881 tcatccgtct catcaatcga catctcgata aaatcgaagt ccatttcttt tacaagggaa
    14941 aaacgttctt tccatgaaat atctttgggt aatgcttttt cataaatccc aatttgagcc
    15001 atttccttac ccccagatac gaatgatctc atctttgaag ttttgtgcgg ccttcatcgg
    15061 atcatttgct gctgtaatac cacgaccagt gataaaggta tacacatcga tcccttcaaa
    15121 taagtgtaat gtgtgtggat ttaatccacc agttacagaa acattaaagc ctagttcaat
    15181 cagttttttg atcttcgata aatctttttc accccaagtt tcaccagata acagcgcatc
    15241 acggctttga tggtagatta cctgccggat accgatgtta tgccaatcca tggcttgttc
    15301 aaaagtccaa ttgccgtaga gttctacttg caattcacct atttcttttt gtgctgcctc
    15361 catagttgga atcgttgcac aacaaatgac tgtcatccag tctgctcctg cttttgcaac
    15421 attactcgcc actgttcctc cagcgtctgc acatttcgta tcagcgacta gttttttatc
    15481 tgggaacata ctgcgaatac agcgaatcgc tttttgtcct tcctgtaagc aaagaattgt
    15541 gcccacttca ataatatcta ctacatcacc aactttcata acatctccta atgcatgttc
    15601 taagtcattg tgatctaacg caatttgcaa attcggcctg ctcatcttag ttccttcctt
    15661 tacttgtttt tttggtacca ctttgtttca gcaaattttt cactgatttc tttggctgac
    15721 atcacatttt tgataccgat aactgtaact tcttttttct ctgcctgagt aaacatattc
    15781 aaaaaattga ctggcgtaaa aactacatcg tattggccag ctgaactttt tccctctgaa
    15841 atggcacaat gatggatttt ggtaattggt acgtttagtt ctttcattgc tttttccaca
    15901 cttctcatca tcatcaagct tgttcctgat ccatttgcac acgatactaa aattctcatc
    15961 ttaatccctc cattatgagt gttgtctaat cagattttct tttatactct ctgcttttct
    16021 gcatatgtgt catagtcttc tacaatcaaa aaataaccct caggattttt ccgatattgt
    16081 aactgcggaa tagccaataa accaatgaca actacggcaa ttccagcata acctaggact
    16141 ttcatgatta ctgtaaacaa tggccaagct gttgcccagt cgaacatccc gatataacca
    16201 ccatatgttg acatcccaat ccatgtagcg aataatgctg aaccgcctac ttgtatgatt
    16261 cctgaaagaa acgggaacag gcaagcggct ttgaatccac ctcggttatt ggcataaact
    16321 gctataacgg cattatcaaa gaacaacgga atgaaaccgg ctatgacgat cgttggtgat
    16381 ttcaataaaa tcagtaaacc aatcatcaaa aactgtccca aagcaccaaa caagaaccca
    16441 atcgttaccg catttggcga accaaagcca aaagtagcag ctacgtcaat tcctggaaca
    16501 gctcctggta aaattgtgtt tgagattcct tggaaagatt gagttagttc atcgacgaat
    16561 gtccgaacac ctaattgcaa gatcgccaga tataccgcaa aatttaaaga agtcgtcata
    16621 atgtagaata agaaactctg tccttcttgc atgaattgcg cttgaatcaa ataatctttg
    16681 cctagtacaa tcagaatgat cccaaagaaa aatagcatga gtaacgaagt agcaaccata
    16741 ttctcattga aaatcgacaa gaatcctggt aatttgatat cttcgatttt tttgtcttgc
    16801 ttgattgatc tatttttctt ttcatcccgc tttttcatcc actcagctaa ttttgcaaat
    16861 ataaagattc cgaacatttg ctgatgtgcc actgcaaatc cggcaccttc tgtcaattcc
    16921 tgcgtgattc ctactgttaa gttagaagct actgcccaat aacaacctaa aatcagtccc
    16981 ataaaaatca atacttgtat cctacctaga tcaggaaagc aaaacagtaa aatccaaaaa
    17041 gcggtagctg cttgttgaac ctgaacgttt ccggtagtga aaacggaacg cagtttcgta
    17101 tatttctgaa aacggactaa taagatattt aaaacaaatg caatcagtaa taaaatcatc
    17161 acatctccaa aggtacggcc aaatgtctcc attaatccag catccacagc attttgtcca
    17221 aaataaggat ctgtaaccat ggcatccaaa ttgaaccgtt cctttagtcc aaccaaaatc
    17281 ggccgaaaat tattgaccaa accaccagag ccaacagtca aaataaagta accgaccgtt
    17341 gcctttaaga atccagcgat gcattcatga agcgggcgtt tcagcaaaat atagcctaat
    17401 aacacgataa aaccaatcag gtatgctggc tgcgtcaaga tatttgttgc aaaatacgtc
    17461 caaatgctta acaaaaattc ccccatcttt tttctcctct atcaattaat tatattctgc
    17521 catcacaact tggtaatcat cgatggagtt cgtagagatg agacgctcaa ccaatccatc
    17581 tgtcattaat aattcggata atttttggat attttctaga tgttcttctg gatcttttgc
    17641 tgcaagtgta aaaaacaaac tagccgtttt ctcctcatcc tccccttcac tttcaaaacg
    17701 gattggctgt ttcaacttgg taaatgaaat agctgttcca aaaacaccat cacttttttc
    17761 tgaagaatgc ggcattgcta cttgaggtgc gatcacaata taaggaccgt atttttctac
    17821 acaatggatg atctcctcca catacgtttc atcaataatt tccttatcga tcaatacaag
    17881 acagctttcg gcgatagctt cacgccaatc cgtgatttct ttatctgggt aatgaatcaa
    17941 ttgatggtca aaaaagtatt tcagcaagaa actccccttc tttcttataa aaacgacgga
    18001 aacggtgtat cattttcaat cgagaagaca tcttcaaatc ctcgataaaa attaaactca
    18061 agatcatctt tgtttgtcgg atagatgaat ttcccgccta cttgccagat ataaggttta
    18121 aatccatact tcaggcgatc ctttttgaat ttccaaattt cagtgatctc ttttgaatca
    18181 gccataaaat tagaccaaat atcgtagtgg acaggaatca ctacttttgc attcaatgac
    18241 tccgccatgc gcagcatatc aacagaagta actttgtctg ttatccctcg tgggttttca
    18301 ccataggccc ctaaacagac atcgattttg tgttcattac catgtttggc aaataaattg
    18361 gagtaatgtg aatctcctgc atgataaaga ttccctcctg atgtttcgaa taaatagttt
    18421 actgcaattt gatccatctc ctgaggcatt tttcctttta atacctcttc tggatcttca
    18481 caagtgacca acgctgtccg atcaaacgct tctaaagcta ctacattgat gtctttgatt
    18541 ttgactgcat cacctggctt tactgtaatc gttttttctt caggaattcc ccatttttgc
    18601 cacgtgcgaa ccacttcttt tggcccaatg aatgttgctt caggacaatt ttgatgaact
    18661 gctgcggctg tatggatatc tagatgatcg gaatggatat gtgtaacgat taaagcgtct
    18721 acatttttta tcgcaaaagg atcgattacg aaaggctgag tacgtaaatt aggctgcata
    18781 ttttgaacgc cactcatacg catcatctga tggccttttt tcatcatgcc attcccatga
    18841 ctacgttttc ccgtcccaca ccatagatca cataaaatat tcgttccttc atgacttttc
    18901 agccaaattc ccgtacagcc cagccaccac atagcaaaag ttccaggatc cactacttct
    18961 gtttcgattt cttcattcaa gtatgtgccc cactcaggaa atgtttctac taaccacttt
    19021 tcttttgtta cttcattgac agttttactc atttgcttgc tccccccata tcgttgttcc
    19081 aacttacaag taaatattaa tgtgtatcag catccatgga aaccctttta tatgaccgtt
    19141 tgttttttat atgattttct ttcagtaatc aatgattgtt tcagtataat aaagacagat
    19201 atgaatgttt aaatgaacaa ctagtcaaat aaatccatgg atcaagatgt ccctcaggag
    19261 gcaaaagtca tgaaaaactc acttgtcaat attgaaaaac gtcaaaaaga aattttggaa
    19321 ctgcttcagc agactgaaca actatcaact ttcgaattag ctgaatccct tgatgtatct
    19381 attagtacga tacgaagaga tttaaattta ttagaagaaa aaaacgagat catacgcaat
    19441 tacggttatt gttcttataa ttacaaaaac cagactgatt ttgatcaatc tggtcctgaa
    19501 aggattaaac aagcgattgc cagagaagct agtaaataca ttaacgatta tgatacgctg
    19561 tttatcaatt ctagctcgac tgcgttgaaa acactggatt ttcttcaatc taaccatttg
    19621 acagtcatta caaacaacgt gaaaatcaat tactctccgc acaaagataa ttgtagttat
    19681 attttaactg gtggagagat gcgctttcca aaagaagcac tagtgggcga catcgcatta
    19741 aatacgattt cttcagtcaa tgcagacatt tgtatcattg gctgtagcgg agtcgatgta
    19801 gtacatgggg tcacaacaaa gatattaaat gaatcaacga tcaacgaaat gatggtcaac
    19861 aagacaatca aatgtaagat tttagtcgct gatcatcgaa aaatcggact gacatcaaag
    19921 ttcaagattg ctgatatttc agtctttgat tatttgatca ctgataaatt ttgttctgaa
    19981 aaagtattag aggagatcag aaatacgggg ataaaaacca ttcaagtaga ttcattttaa
    20041 gaaccaggac gtaaatatgt aaataggatt atcaaaaaaa tgaataacaa atttatcgat
    20101 aaatgaaaaa cacgctacgc tcaaattgat aaaggaatcc caagatgtgt ccatccaatc
    20161 gataccatca taatctattg cttcttttat aaccgttata tgttgcctaa ataggaatca
    20221 aacaatatcg aaaaaaggag gtatgacaaa agtcacacct ccttttttcg aataaacggt
    20281 attcaaatca gaacactgat ttcacaacca ctttatttca ggtcgttttc aatcaccttt
    20341 accgcgtaat catttccgtt gaaacgcgct attttttttg cgtccatata tctttctaaa
    20401 taatcatctt ctcctaattg cttatggcaa gaagcaattt cataaagcaa gtattccaat
    20461 gcataagtcg atttatgttt cttattatac tcaataccct tctcacataa ttggattgct
    20521 ttatcataat cctcgatttt acggtaatag gtggcgatat cataatatac aatgcagacc
    20581 atcagattat caaaatcagt ctcactcaat attttaagtg tacgctccac ataaactttc
    20641 gcaaaatcta attctccttt tgacaagtag atcattgcca cgtttgcagc tgcttgtaag
    20701 tgatggatat ctgtttcggt cgtttgttcc aacacttgat taaaataaaa taatgctgca
    20761 tctggttttt tcgcataaag atgatcaagc agtccttgat aaaataaaat ttgatttaaa
    20821 agatcagaat tacttacttt ttcctgatcg atcgtttgta aaattttata agattcttgg
    20881 tattttgata cagaattcaa tgattgaatc tcatttattt ttttcctgat tttgtcttct
    20941 tctgtttcta ctaatatatc ggagatctcg acattcagct ttttgcaaag cgcacttaag
    21001 atattgatgc ctggcgacat attcttgttt tcgatattgc tgacagttgc ttgttcacaa
    21061 atgccttctg ctaattgtac ttgtgttaac ccctttttct ttcggtattc ttttagtaat
    21121 gctccatcta ctttaatcaa aacaatttcc ttccctttaa aaaattattt ctcctttata
    21181 tcatacacaa aaaaaagttc taaacaacca aaaatgttta gaacagaata aaaattttaa
    21241 ttttaaaata atattcaaat taaacaactg gcgtatctgc tccttgattt ctttttgtgt
    21301 tatctgaata attatcgttt acagttataa cacatagtga aaattgtcat ggcgagaaag
    21361 tctaagaata tttttaaaca taactatttc tttcttacgt gtgtataatg acgacagcac
    21421 acctatcaat taatataatt attttatttt ttatgttctc atgtttattt ttaatgacac
    21481 aacttctcgt atttatctgg cacaacatat aaatcgatca aaaaattgtt gcgtgttttt
    21541 ttgaaaattg gagtttcaac ttgattacaa aatttaaatt tagaagttgt gaaaacagcg
    21601 agagacagtt taatattcaa aatatacata ggctactcga acgctgtgtt ttttagtata
    21661 ctagtaagaa acgaagggat agactcattc caataaggag gatttcatga agacaattct
    21721 ttttgctcct gcagtattca atttagctga gacaacacga atgattgagg ttgccaaagc
    21781 tatgaaagac cgttattact gtgtattctt tggcttttct aaaacttttg ctcctttgat
    21841 cacagaaagt ggctttgagt atcatttact agcgccgatc cttacgaaaa aacaagaaga
    21901 acaaatcata aaatttgacc agttgcgtac acttaaaaac ccttttacca aagaaatggt
    21961 tgcctctcgt atcactagtg aacagagtct gattgctaag tttgagcctc gactgattgt
    22021 gacaggttcg aatgtgacaa tttttttatc ggcccgcaat aaaaagattc ctttagtata
    22081 tgttaaaccc tatgctttga gtgaaccttt ttttcaatca gagcatgcgc tgatgcccca
    22141 cgagttacgt ttcttcggcc ctttaagcaa tcccctttgg cactggacaa aaaagcgtat
    22201 agtaaatacc cgatggatcc ccaatgcctt caaacacgct gcacaaaaag aaatgcttcc
    22261 tttaccaaaa acaagtttac aaatgatgga tgctgatcta aatctcatca caacgcctaa
    22321 actatttttg gaagaagaac agctgccgga aaattatcga atcgttggtc caatttttgc
    22381 taagctcagt acttccttgc ctgaagaagt gctaactttt atacagcgaa agcaacaaga
    22441 aagaaaacgt attatctatt tcgcaatggg tagttcaggc aatccgcgta tgttacgcaa
    22501 aattttgttc ttcttacaaa aacaaccgga tctagcggtt attgcaccta ttgactatct
    22561 gcttgatgac attcaatctg actcagaaga tcttttaata acagaatatc tgtctagtca
    22621 tcttttgcat gaattcattg atttttcttt tatccacggc ggggaaggga ccgtccaaac
    22681 tgcctgtgaa tccggcaaac cgtttatcgg tgtggggatg caatttgagc aaagctgtaa
    22741 tattgactat tgtgtaaaat acggaaacgc aatcgccctc acaaaaccag tgactgaaag
    22801 aaaactgcag attgctctgc gcgaagtttc ttcacccaag attcgtcagc aggcacgcac
    22861 gctgcaagcc gtttttccaa taaatggtcc acaacaagca attcaagaaa tcgaaacctt
    22921 cctgagcaac cacccaatat agtttcaagt agaaaaagct tttttaatag agactcatca
    22981 gtaatttcgc tattcgagac ccagacagtg aaaatacaaa aaaagtaaat agtggattga
    23041 aaagttagag ttacttaaat tacggtgtct aaaaaacaca aaatcatcct taaaataaaa
    23101 gatctgacgc aatccaaatg agcataggta gcgtctgcca tttattctta gtcaaattct
    23161 ttgacacctt tctctggttg gatcatctca acgtttcttc aaaagaaaga ccataataat
    23221 taggcaaata cattttctta aaatgtgtca tatagatcta gaagcatcgc tttgtagaga
    23281 tttgtgctgc tagtcccttc aacaattact aaatcaaatg taggatttac cactttatct
    23341 ttgataatca acctccctct tctgttgaga caatgatctc gcaatccgat gcatactcct
    23401 atataaactg ccagcaatcc ccacatgctg agtagatcat gtcagaatgt tctatgtcgt
    23461 cagcaacgat aaactaaaag tcacgttcac catgactgat tgctattccc agcgcaatta
    23521 tttctataca cgtttcatga tttgaataaa cactctcatc atacatatta ccattcatac
    23581 accatactgc agcccctact atatggttgt aagctcccaa atctatattt gccttgatca
    23641 ctttggtagt agatcgacta attcttaatc cattgctgat aaaattttca tttttgcctc
    23701 cttctttctg ccaaaacagt taaaacaata ataatcttta tgtaaaaaag ggctgtgaca
    23761 aaagccttgt cacagtccca tattccgaat aaacggtggt caaaagcttg catctgcggt
    23821 aataagttca accaaacgaa aaataagcca acatttcaca aaaatttaag aagcaatttt
    23881 cgtaaatttc ttcttatttc ttgaagctaa ccacttctgt cacaaccact tttagaacat
    23941 ttaaagcttc gatgtgacaa accatctatg ctgctcgatc ataaaattga tagcaaaaat
    24001 tccacaaagt acatatgtcc cccatgaaaa caaaacattc atatgcggta caatgatctt
    24061 tggaagaatc acgatcaaaa atgatgccag tgcccaaatc catcgattga tttgaatatg
    24121 atatgtagct agcatattca atataaggtg actagccgtt aataaaaaga taaaaacaaa
    24181 aaataaaatc agccaataca ttgtttccca ccttctaaaa accaaactta gacagtttgg
    24241 acttcgtttt ctccacgact ttgttcatct gacagcatct ttttatcata agcttttaca
    24301 aatggataat agataataaa tgctatcaat gtgttgatca tgatcaagat aacaacacgc
    24361 cagtcatttc ctgctgaaag ataaccgccg attggagctg gtaatgtcca agttggcaaa
    24421 acagtaaatc tattgaccat ttctaatttg actgcaaaat aactgataat agtcaaaact
    24481 aaaggaacta agttgaacgg gatgaacatg taaggattca acatcatagg agctccaaaa
    24541 atcatgggct cattgatatt gaagatacca ggaataatag aaaaacgacc aacctctttc
    24601 aagaattttg atttacagaa gaatataaac aaaacagata ccacgatcgt tgctcctgca
    24661 ccaccaattg ttaccgtcca ttgataaaac tgttcaggag cgatataggg taattgggtg
    24721 gcagctgtac catctgccaa tgcctttgca ttctcatcca acatgatcag ccacattgga
    24781 cgaacaacag cccctacaat actcatgcca tggataccaa atgaccacaa caaatggatc
    24841 aaaaacattg gtagtaaaac accaagcaaa ttattcccta aaatattggt taaaggctcg
    24901 aataacgata ataaggtagc gtttacatca aagcccgcga cgcttcgaat cagccaagta
    24961 attgttaaaa tgactgcacc tggaatcaat gcctcaaaac tacgggcaac agcaggtggc
    25021 acttgttctg gcattttgat agtaatattt cgatttttaa agaaacgata tacttccact
    25081 gcaaaaatca tagataagat ccccgcaaac atcccagatg ctcccaaaga actcataggt
    25141 aagacccagc caagaggtgc cttttcagga gttaaaacgt ttacaggaac ttgtaacata
    25201 aagaacgttc ctaaagataa tataccacct gtcacactat ctaatccata tgatttagct
    25261 agatccgaac ccataacaaa agttgcataa atagccatca atcccatcgt catcctaaaa
    25321 ggtaaaacaa tcgttgccga ataaggggca ataaattcat tccatccagg aaacggtaaa
    25381 ttgataaaga ccataaaaaa tgtcccaatc aaaatcaatg acaatgttga aacaacccca
    25441 ttacgaattg ctaataaatg acgctgtgta ccaatttttg ccattggtgg gacaagtttc
    25501 tcttcgaccc atttcatcag cttttccact aaaaccactc cttttcaata aaatatgaac
    25561 cattcattgc atgacaacgc aaagaatgtt cataactaat tatataatta agaaatttta
    25621 tttcaatata ttctcttaaa taatcaaatt tatttttaag aatcctgaaa ataagttttc
    25681 taaatcatag gagcaacaaa aaaattcgat aattaacaaa aatcaaaatg attaaatcta
    25741 gtgattgaaa cttatcattt caaaaaaatt ttgtaaattc acccataaat atcaccctat
    25801 ttaacttttt tcatcgtaca ctaaaattca aaacaactat ctttaatctt tacttcttgg
    25861 ttttgtttta ataaatttcc ttcaaaaatg cctggagaaa ctacaatgat caccgtatct
    25921 ttttgtgctt gctccaactt cttcaaatct ataatagcta acagtgtccc ttcttttacc
    25981 tcctggttac tttcaacccg tatctgaaac ggacttcctt cgagttcaac tgtatctaaa
    26041 cccatatgga tcagtatttt tctgccagat tcactttcga tcgttatggc atgtcgggta
    26101 ggaaatatat tatcgattat tcctttaact ggcgcatgga tatatccatc atgattcgtt
    26161 atagcgtatc caactcctat catttttgaa gaaaagacct cgtcttttac tgctgttaat
    26221 ggaataattt gaccactagc tacagctttt agaatgtttc ttgttttaaa gaaatgcact
    26281 ttttatcacc tctactttaa tgctggccaa tattttttgt ttactttgat catttcatct
    26341 agtatttttt ttgcgactct ggcatttggt acggtttgat tcaatgtaaa tgcttgtaaa
    26401 gctttttgat aagaaccttc aaagtaagca tctactagta acttctctgc tgcaacttgt
    26461 gcctccatca atcctttatg aaaatcactg attggttttc ttagtgtaat tgtctccaca
    26521 ccagttgcac caacatatgc cggtatttca acaactgcat ctgaccgtag attcggaatc
    26581 gccccttgat taggaacgat tagcataaag cgacgacgat catcattcaa caaggaaata
    26641 gctatatcga cgatatattg tccatgctcc ccaaaattaa agttcaatac ttcttccaca
    26701 ataccgcggc gtattttatc agccatttct ttggtatttt tctctcgtcc atccatgacc
    26761 atattagcac gtgtatactc tttgtcagta tgctcaacga ccatatcagg atataagtaa
    26821 tactctaagt aattattcgg gatattactt ggaaaatttt tcgtgatgac tgacatcatt
    26881 tgaaaggttt tttgccaagt tttgtctcct atattaaaat cggcgacttg catttctttt
    26941 gttattagtt tttcaataat ctctggcata atttctcgtt gcaattcttt atcataaatc
    27001 gatgtatacc agccaaagtg gtttaaacca taatacgtaa cgatccaatt cttacgatca
    27061 taaccgtaat tcactgcaat cgtttcttca attgaaatag tcatatcaca ggcattaatc
    27121 atttttacat tcggatattt acgacgaaca gcttcagaaa cgatcgactc tggatttgta
    27181 taattcaaaa tccatgcttt gggggcatag tcttgtatat gatcgacaat ttccaataaa
    27241 cctttgattg agcgcatccc ataagcaaat cctccgagtc cacacgtttc ctgtcctact
    27301 aatccatgtt taagtggaat cttttcgtcc ttttctcgca tctccaatcc tccaacacga
    27361 atttgtgaga aaacaaaatc gcatcctgta aaagcaagct ttggatcatc cgttgcacga
    27421 atgcgaatat ttgaaaaacc ttttttttca gcataaattt aacaatcaag tacatatcca
    27481 tatttctttc ttcatcaata tcatataaac gtatttcaga taatggcaac cgtttttcat
    27541 tgttcaaaat ggcttgaacg attcctggtg tatacgtact cccgcctcca gctatagtga
    27601 taatctgttt tttcatcatc atcttttcct ttctattctt ctaataattt attgaattct
    27661 gccataacat aagtcacttc taagccgatg ataatttgat aatttttttc agttggttta
    27721 atgatcccgc ttatccctaa ttgcttgatt cgctgttcct cgaccatggt gggatccact
    27781 acctctaccc gtaggcgtgt attacaacta tagacatcca ggatattggc ttttcctcca
    27841 aacgcttgta gcatttttgt tgccatatat tcataatttt tacttgctaa ccctaactct
    27901 gtttcttcta aggttacttc attgccaaac tctgccttag cttcacgtcc taatgtctga
    27961 atgtttctct tattgatcaa ataataaaaa gtaacgtaat acaaggcaaa aaagaaaatt
    28021 ccgattggaa gaatcaaaat agcattggta gcgattcgaa aattgatgag ataatcaaca
    28081 atacatgctc cgaatgtaaa accaattcgt atattgaata agtaacagac aatccctgct
    28141 aaaccggcaa aaagtgcatg gatcacgtac aacataggag aaagaaacat gaaactaaat
    28201 tcaacaggct cagtgatatt tgcaataaat gaagttcctg caccgcttcc catgattcct
    28261 ttgaccatct ctttattttc tttataagct gctttataga tagctagaca tatagctggt
    28321 ataccaaaca tcattactac aaaaaaacct gataaaaaca gtccagcctg aggatctccg
    28381 ttgataaagc gtgtcatctc tcctctgaaa acttccttgc taggagatgt atagcttcct
    28441 aaatcgtaat aaatatacgt attcaacaca tggtgcagtc caaaggggat cagcaaacga
    28501 ttcaaaaaca taaatatccc cacacctaat gcccccatat caacgagcag ttttgcaaaa
    28561 ttatcaatcc caatttggat gagcggccag ataaagccca aaaaaatagc tgcgattgtt
    28621 tgtagaatca tcaccatagt caatgggaat ttttcgcccg caaaataaga aaaaactact
    28681 ggtaattttt gttctttaaa gcgattatac gtccatgccg ctactaaacc ggaaataatt
    28741 ccaccaaaaa tccccatatt cacttctggg tccatgattt ctaagccttt tttcaaggta
    28801 gcaatcgcta aaaaaccagt aagtgcaggt attcctttgt ccttattttt gacaaaaccg
    28861 attgcaactc ctatagcaaa taagatatcc aagtttccaa aaacggcatt tccagctgca
    28921 ccaattactg ggatgttcag catatcatca gctgataaac gcatgatcaa tcccgctaat
    28981 ggcatcgctg caactggaag tagcatcgct cttccaagct tctgcacatt tgatttaaca
    29041 ttcattttta ttcctcctct attaaatatc ctcatatgtt actaactcaa tcaaaggatc
    29101 atttttcact ttttgagcaa atgctgaatg aattaaaaat tcgacttggt gagtacgagg
    29161 atacacaaaa cttgaaccat ttaacaattc ctgatccaaa tacgctgcgt gacacatgac
    29221 ttcgatcgtt ttatcttctc tcaatatcat gagtaaactt gccaaatatt cttccacact
    29281 cgtattgatt tgtcgttttt tttcatcagt ttctcctacg acatcgaaac aaggctcaaa
    29341 ataatcgaca tgacgaactt catcttttcg ttcaaagttg ctccgaaccg gcaaatcata
    29401 tttttttgct aatgtaacaa ctacctcctg gttttcatcc aaagtatgag tatgatgatg
    29461 actgtctaaa tgagttggta tgatccccgc atgataaatt ttttgaatct gagcattcca
    29521 ttcctgatac agttgatctt tgtcaatgtt aaatggatgc tgataataag ataaggatct
    29581 gaactctccg ctttcgataa gagtatcgac agttttgaga agtggtttgc cacacgtcaa
    29641 tgttaaatgg actcctacac ctagcctagg catttccttt cttagtttca cagcgtgctc
    29701 gaaacccggc atatttgcca tcaatgtagt tgatgtcaaa attcctcgct gataagcatc
    29761 cataatccca taattcacac catggctata gccaaaatca tccgaattaa aaatcacttt
    29821 tcccatttca ttctccttct tttttctact aaaataggcg caaccaaata atcatagtcc
    29881 atttactatg tgtcatttgg ttgcgcctat agatagttac gcccttattt taatagattc
    29941 gtgctagatg aattgccaaa tagatgattt cattttcttg aatggcaagt tgaaattgct
    30001 gttcgatgat atctttaatc tttagtgctg tacaatacgc tttgctatgt ttaaatgttt
    30061 cagtaaaaaa accaatattt tctactggcg tttcttcctg ataaatcaag cgtttaacca
    30121 ggaatttgat atgaaccatt aagcgatgat aagtcagtga ttctttatca taaacgtttg
    30181 aataggattt ttctagatgg gacaaaatcg tggtcgtcat ttctactatt tttaagctct
    30241 ttaaactatt aggctctcct agctcagcat taatgatatg catagtgatg aatcctactt
    30301 catttgaccc taactcggtt tgatatcgat gattgattcg ctcgaccact tctattgcta
    30361 tatcatattc atttggataa aggaatctca tttctggcaa cataattgct gtgacatctc
    30421 tatcttctct ttttcgttga atagcaaaat aaatatgatc tgtcaatgta atatagatat
    30481 ttgaatctaa tggagaggag atcctagctg ttatcagttc aatcacttct tccgcaaaat
    30541 ccataaattc gattggaatc tgcgagacta tctcactgaa tcgtttgatc ttttctttag
    30601 aatctaatac aaataatttg tcagcatcct caacatcaaa tggcttactc actttagcag
    30661 aaaagccaat ccctttcccc atcgcaattt tttctattcc atcgtcatcg atcaatacag
    30721 cattatgatt cagctttttc atcacataca ccgttaattc acctccacta tatgataaaa
    30781 caaaacaact tgcagactcc ttcatacata tttttgtctc caaacaattt ttaaagacaa
    30841 aaaacgacct aagaaagaac cctttcttag gtcgtgccta attgaatagt agcaccctac
    30901 ataatttaca aaaatagttt aacacataat aaaagcgtta tcaatatctt ttttactcgt
    30961 tttatgataa tacttgtatc gaattgattt ggaggtaatt acataaaaac aaaaaaaata
    31021 tagcaactct ctataagaaa gttgctatat tgataattat accggcggcc ggggtcgaac
    31081 cggcacgtcc tcaacggaca ctggattttg agtccagcgc gtctgccaat tccgccacgc
    31141 cggcaaattt aaaaataata actggggtag ctggattcga accaacgcat gagggagtca
    31201 aagtcccttg ccttaccgct tggctatacc ccaagaataa agggcgaatg atgggaatcg
    31261 aacccacgaa tgccggagcc acaatccggt gcgttaacca cttcgccaca ttcgccatca
    31321 taaaagatgt tatttaattt ctcaataaaa aactaagatg aagacatctt agtcaatacc
    31381 ggcggccggg gtcgaaccgg cacgccctca acgggcactg gattttgagt ccagcgcgtc
    31441 tgccaattcc gccacgccgg caagttgatt tattagtact aaggcggtaa ccggatttga
    31501 accggtgatg aaggttttgc agacctctgc cttaccactt ggctataccg ccattatact
    31561 attatcaatc tacaaaataa agaataaatt atagaaactg gggtagctgg attcgaacca
    31621 acgcatgagg gagtcaaagt cccttgcctt accgcttggc tataccccaa gaataaaaag
    31681 ggcgaatgat gggaatcgaa cccacgaatg ccggagccac aatccggtgc gttaaccact
    31741 tcgccacatt cgccatggca ggggcagcag gaattgaacc cacactaacg gttttggaga
    31801 ccgctgttct acctttaaac tatgccccta taaaatggag gagagtggat tcgaaccact
    31861 gaaccctaag gaacggattt acagtccgtc gcgtttagcc acttcgctac tcctccatgg
    31921 tggcgcggga cagaatcgaa ctgccgacac acggagcttc aatccgttgc tctaccaact
    31981 gagctaccgc gccatatttt aaaattatta attgaataac aagaacgacg gttccgacgg
    32041 gaatcgaacc cgcgatctcc tgcgtgacag gcaggcatgt taacccctac accacggaac
    32101 cagttcatta tggaggttaa cgggatcgaa ccgctgaccc tctgcttgta aggcagatgc
    32161 tctcccagct gagctaaacc tccaacattc aattaaaatg acccgtacgg gattcgaacc
    32221 cgtgttaccg ccgtgaaagg gcggtgtctt aaccgcttga ccaacgggcc aggtaaattt
    32281 gctacggaga gtaagggatt cgaacccttg agacagtttg caccatctac atgatttcca
    32341 atcatgctcc ttcggcctct cggacaactc tccagagtga aagaagctca atactctttc
    32401 tcaaaatgag
//
LOCUS       DAZABD010000032        30236 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000032 DAZABD010000000
VERSION     DAZABD010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30236
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.032"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(79..2154)
                     /gene="glyS"
                     /locus_tag="ACHALG_001511"
     CDS             complement(79..2154)
                     /gene="glyS"
                     /locus_tag="ACHALG_001511"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFN2586912.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEIIPFEILDVKTDRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     KEMIITQANELAEKNHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDADRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELYKAVEG
                     MKTSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLNQIAAMALSL
                     ASLDQIITK"
     gene            complement(2156..3073)
                     /gene="glyQ"
                     /locus_tag="ACHALG_001512"
     CDS             complement(2156..3073)
                     /gene="glyQ"
                     /locus_tag="ACHALG_001512"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFN2586913.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            complement(3457..4269)
                     /gene="recO"
                     /locus_tag="ACHALG_001513"
     CDS             complement(3457..4269)
                     /gene="recO"
                     /locus_tag="ACHALG_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFN2586914.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            complement(4412..5311)
                     /gene="era"
                     /locus_tag="ACHALG_001514"
     CDS             complement(4412..5311)
                     /gene="era"
                     /locus_tag="ACHALG_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFN2586915.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5326..5721)
                     /locus_tag="ACHALG_001515"
     CDS             complement(5326..5721)
                     /locus_tag="ACHALG_001515"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFN2586916.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            complement(5705..6181)
                     /gene="ybeY"
                     /locus_tag="ACHALG_001516"
     CDS             complement(5705..6181)
                     /gene="ybeY"
                     /locus_tag="ACHALG_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFN2586917.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6198..8390)
                     /locus_tag="ACHALG_001517"
     CDS             complement(6198..8390)
                     /locus_tag="ACHALG_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing metalloprotein"
                     /protein_id="HFN2586918.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKVNDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVVFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            complement(8414..9385)
                     /locus_tag="ACHALG_001518"
     CDS             complement(8414..9385)
                     /locus_tag="ACHALG_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFN2586919.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10091..11470
                     /gene="brnQ"
                     /locus_tag="ACHALG_001519"
     CDS             10091..11470
                     /gene="brnQ"
                     /locus_tag="ACHALG_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFN2586920.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHYVPFAALGLGWVVPTLLGYAGSLLFYYVYRVSGYK
                     QEASELPEE"
     gene            complement(11624..12070)
                     /locus_tag="ACHALG_001520"
     CDS             complement(11624..12070)
                     /locus_tag="ACHALG_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFN2586921.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(12097..12273)
                     /gene="rpsU"
                     /locus_tag="ACHALG_001521"
     CDS             complement(12097..12273)
                     /gene="rpsU"
                     /locus_tag="ACHALG_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFN2586922.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            complement(12435..12863)
                     /locus_tag="ACHALG_001522"
     CDS             complement(12435..12863)
                     /locus_tag="ACHALG_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFN2586923.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            12955..13815
                     /locus_tag="ACHALG_001523"
     CDS             12955..13815
                     /locus_tag="ACHALG_001523"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFN2586924.1"
                     /translation="MTSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            complement(13907..14257)
                     /locus_tag="ACHALG_001524"
     CDS             complement(13907..14257)
                     /locus_tag="ACHALG_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFN2586925.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKSKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(14272..15999)
                     /locus_tag="ACHALG_001525"
     CDS             complement(14272..15999)
                     /locus_tag="ACHALG_001525"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFN2586926.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EENHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAKKVASYE
                     AIIKKMTEE"
     gene            complement(16214..17140)
                     /gene="trxB"
                     /locus_tag="ACHALG_001526"
     CDS             complement(16214..17140)
                     /gene="trxB"
                     /locus_tag="ACHALG_001526"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFN2586927.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            complement(17285..18718)
                     /locus_tag="ACHALG_001527"
     CDS             complement(17285..18718)
                     /locus_tag="ACHALG_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFN2586928.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDVFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEKGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWDSRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            complement(18718..20121)
                     /locus_tag="ACHALG_001528"
     CDS             complement(18718..20121)
                     /locus_tag="ACHALG_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFN2586929.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLDIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETITSIYGNKETQIV
                     VSTKNDGAVPDLSADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETIGRLKEEGIEVQ
                     DELARELSSNTHIMEEV"
     gene            20352..21254
                     /locus_tag="ACHALG_001529"
     CDS             20352..21254
                     /locus_tag="ACHALG_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFN2586930.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKYLLLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNDVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            complement(21294..23180)
                     /locus_tag="ACHALG_001530"
     CDS             complement(21294..23180)
                     /locus_tag="ACHALG_001530"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFN2586931.1"
                     /translation="MGKYQELASKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGIVTVMRAGGQYQVVIGNHVRVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAAGGMIRGLTALLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTVARKFKVNEFVGMLLGAALMNPNLSLEVLSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPAFTFATDILMAGFETLLALSPVNYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAMPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKDEAGETDDIQKRSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSETGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(23275..24126)
                     /gene="licT"
                     /locus_tag="ACHALG_001531"
     CDS             complement(23275..24126)
                     /gene="licT"
                     /locus_tag="ACHALG_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFN2586932.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEDLQVHLNDSVYIALTDHLY
                     TAIERQKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDKAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRCYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GAKSEDGEVSEELFQVIQKKYKDTFGCVEKIADFLTGAFDYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            complement(24452..26236)
                     /locus_tag="ACHALG_001532"
     CDS             complement(24452..26236)
                     /locus_tag="ACHALG_001532"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN2586933.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIITRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTSDTETLFEFWYVFLMVLTGI
                     FAVVSSFIAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMMKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPIEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVEHGNHETLLAQNGLYADMYKLQSSGI"
     gene            complement(26236..27987)
                     /locus_tag="ACHALG_001533"
     CDS             complement(26236..27987)
                     /locus_tag="ACHALG_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFN2586934.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLMLDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            complement(28129..28353)
                     /locus_tag="ACHALG_001534"
     CDS             complement(28129..28353)
                     /locus_tag="ACHALG_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HFN2586935.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            28604..30190
                     /locus_tag="ACHALG_001535"
     CDS             28604..30190
                     /locus_tag="ACHALG_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFN2586936.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
BASE COUNT         8430 a         6477 c         5363 g         9966 t
ORIGIN      
        1 ttatttatat ttataacaaa taaaccagag aaacagccta ttttgtgcct cttctctggt
       61 ttatttacat attagaaatt attttgtgat gatttgatcc aaacttgcta gtgacaaagc
      121 catggcagca atttgattta attgcgttaa tcggttctta cgtactgctg gttcttctgc
      181 catgaccatc gtctgatcaa aataattttc gatcagtggg cgaaggctta caagctgatc
      241 ataattttcg ttgatcgttt ggtcagcaaa gcttgttttc attccctcta ctgctttgta
      301 cagttctttc tccgcttcgt tttcgaacag ttcagggtct acttctgatt gtttttcaag
      361 ctccgctttt ttcgctaagt tcacgacacg tgtcaaagct tccatcgatg gtttgaagtc
      421 ttcatctgca agatgctgct tgaacaattg tgctgctgca aatacttttg tcagatcttt
      481 ttgttctgcg ttcaaaacag cttcgatgac atcatgacga atattcttcg ttgtcaataa
      541 ttggcgcaga cggcctttga taaagtcgat cacctgttgt tgtcccgctg acaagccaat
      601 gccaaaacga tccgcatctt tactgataac ttcactaatc gtttcttgca gaacagataa
      661 aggaaaggcc cattttttcg attcgatgat tcgaacgaca ccatatgctt gacggcgcaa
      721 agcatatgga tcgtttgagc cacttgggat catcccaact gcaaagaaag aaaagatact
      781 atctaattta tcagcaatcg ctaaaacaga acctactgta gtctcaggta gatctccttc
      841 actggatata ggcatgtaat gttcacggat tgctgttgct acggctggtt tctcgccttt
      901 aagcagagca tatttttctc ccatgatccc ttgtagttca gggaattctc ctaccatgtt
      961 ggttactaga tcaaatttat agatttcagc agcgcgttgc agatccatca attcttcctc
     1021 agatagtcct acttgttcgc ctataatgcg ggcgatgact tgtacccgtt gcattttctc
     1081 gtagatgcta ccgatttttt catggaaagt aacagttttt aatttttcaa cgtattggtc
     1141 gattgaagct ttcttgtctt cattataaaa gaattctgca tcttctaaac gagcagtcaa
     1201 tactttttga ttgcctcgta ccacattttc caattttacc cgatcgccat tacgagcaga
     1261 aataaaatga ggaagcaaca taccttgatc gttccgtact tcgaaataac gttgatgttc
     1321 tttcatagaa gtgactagta cttcttcagg aacagaaaga tatttttcct cgaagtcgcc
     1381 agaaaagaca gttggatatt cgactagatt gttgacttct tctagtaaat cttcatctag
     1441 atcaatgatc cagtgatttt tttctgctaa ttcgtttgct tgtgtgatga tcatttcttt
     1501 tcttttttgc ggtgaagcaa taacaaattg ttcatttaat ttttcttcgt attcttttgc
     1561 atgcgcaaat gttacatctt cgcctaagaa acgatgacct cttgaagctc tgtctgtttt
     1621 gacatccaag atctcaaacg ggattatttc gtcatctaac aatgccacga tccagtgaat
     1681 agggcgaata tattcaaaat cgtattctgc ccagtgcatc gttactggga aagtgaggct
     1741 tttaatgaca tctgctaaac ctgtcaatac ttcttttgct tctttgccgt gagtgtattt
     1801 cgttacatag acatattcca cgccttttaa ttctctaaaa gtgatagcat ctgtcgtcag
     1861 accttgtccg cggacaaatc cttcagctgc tttcgtccag tttccatctg cgtccaaagc
     1921 aatttttttg gctggtcctt tgacttcttc ttcactgtct gcttgacgtt ctggaatgtt
     1981 tgttactttg attgctaagc ggcgcggtgt agaaaacgtt tcgatcgtat cataagtcaa
     2041 ttggttttca tctaaaaatt ttgctgtttt ttcttctaat tgtttcatgc ttggtgtgac
     2101 aacatgagca ggcatttctt ccagcccgat ttcaagtaat aagtcttttg ccatcttatt
     2161 cagcctcctt catgttttct ggatttgctg tgtttttatt caataaaggg aatcctaatt
     2221 tttcgcgttc tgccacaaag attttggcta cagcacgagc catgttgcgg atacgggaaa
     2281 gataacctgc tcgttctgtt acggatactg caccgcgagc atctaataag ttgaatgtat
     2341 ggctgcattt caaaatataa tcatacgctg gatgaacaag attttcatcg atacagcgtt
     2401 tagcttcttt ttcaaaacgg tcaaaattgc ttagtagcaa gtcttggtcg cttacttcaa
     2461 aagaatattt ggaatgttca tattctggtt gtttgaagat ctcgccatat ttcacaccat
     2521 cagcccattc cagatcatag acactttcaa cttcttgaat atatgaagca agacgttcta
     2581 gtccataagt gatctcagaa gtaactggtt tacaaggtag tcctccgact tgctggaagt
     2641 acgtgaattg tgtgatctcc atcccatcaa gccatacttc ccaacccaca ccggcacacc
     2701 ccatcgatgg gttctcccag ttgtcttcaa caaaacgaat atcgtgttct aatggatcaa
     2761 ttcctaaaag acgcaagctt tctaaataaa gctcttggat attttcaggt gaaggtttca
     2821 ttacgacttg aaattgatga tgctgataca aacggtttgg gttttctcca taacgcccgt
     2881 cagctggtcg gcgggatggc tctacataag cagcattcca tggttccggt ccgattgccc
     2941 gtaagaatgt atatgggctc atcgtacctg ctcctttttc tgtatcataa gcttgcatca
     3001 gcatgcagcc gtttgatgac caaaattttt gtaatgttaa aatcatatct tggacggtca
     3061 attttttact catctgtttt gcccctttca atttttaagc gttgctgtca ataagaatac
     3121 aggaaagaaa acgacaaaaa gtccctatgt ctgattacag acatagggac gaataatttc
     3181 gcggttccac cctacttccg aataaagatt cggcagcttc atttatcgta ctcgcaagtg
     3241 cctcttcaaa aatcgtttgg gtccggcttc cactctcccg aactcgctaa accacgtgtt
     3301 ttttactgat cttggtcaat gtacgtattc attttgcagt tttcttgttt atataataga
     3361 tcctctcagt gaacgatcta tttaatctac ggttaataat agacggaagt ccctgtattt
     3421 gtcaaatagt tttgattttt ttgttgtatc tttcatttat tcttcttttt caggtatctc
     3481 tttttcaggc atatttttct ctggcgtctt taatacgtgt gcccaagact tcatttgatc
     3541 aatgaacttt ttgcttttta gatgaatacc gacatattca tcatacaact ggtcaatgag
     3601 gtcacggatt gcctgcttcg tttcttcttt caagtggatt ccactgatct tatcataaga
     3661 aatagccgaa aacattcgga cgaaataaac agcacgagga tctgcatgat aacggtgttc
     3721 gtccattggc cagtgtttct cgcatatcac accgccatat ttggaagaat agtcaaattt
     3781 tccttttgtt tctccgcata cgacacagtg agtccaaatt ggcgcgattc cgaagcgttg
     3841 cagtatttga acttcaaaga tattcgtgat gatttctgca tcatctcctt gatccatccg
     3901 ctgcagagct tgttgcgtaa attggaataa atgcgggtca tattgatgat cttcgattgc
     3961 tgcatctact agatttagca aataggtacc ataaccgctc aaaaagatat cttcttggat
     4021 gttccgaaac gcatggacgt ctttgacact gcttaaaaac gacaaacctt cacttcgaaa
     4081 attaccgata tagactgctt cggtaaaggg gagcaaagcc ggagctaatg gctgattttg
     4141 ccgatggatt cctttgatat aaaacatcat tttgccagaa gattccgtaa agattttgac
     4201 taatttatct ttttctttgt aattacggct agataaaatc aagcctttcg attctgcaag
     4261 ctggctcata ttctccctcc taaaaaataa atctggaaaa gcagattctg ccgatccaga
     4321 ttatttatgt ttcgatcttc cgccgttttt ctcaacgtgc ggatttttat ccagtttatt
     4381 tctatactta acctataaga agttcagaat atcagtattc atcttttcgg tagccgaagt
     4441 cttgcagata gacttttttg tctcgccagt ctttttggac tttgacccaa agttctagaa
     4501 atactttgtc tcctaaaaga ttttcgatat cctttcttgc tttcgttcct acttgcttca
     4561 gcatttttcc gcctttgccg ataatgattc ctttttggct atctctttcg acgatgatcg
     4621 tggcctggat atggatttta tcattctcgt ttcgcttcat cgaatcaaca acgactgcga
     4681 cagaatgagg cacttcgtct cgtgttaaaa acaagacttt ctcacggatc aattcagaaa
     4741 cgatgaagta ttcaggatga tcagtcactt gatcatctgg aaaatattgt gggccctctg
     4801 gcatttcact gactaataca tccattaagc gttcgacatt gtttccttct gtagcagaga
     4861 tcggaatcac ttgtgcaaaa ggcatctgcg tcgaataatc ttcgatgatt cccagcaact
     4921 cgtctggatg gatcttatcg attttattga taatcagata aacaggagaa tgcacgtttt
     4981 ttagacgttc aataataaaa tcgtctcccc ggccgcgttt ttgatcagca ctgatcataa
     5041 ataagactgc atccacttct ttcaacgcac tgtaagctgc ttctaccata aaatctccta
     5101 gacgatgctt tggtttatgg attcctggtg tatcgataaa aatcagctga gcttctggtg
     5161 tagtatatac tccttggatt ttatttctag ttgtctgtgc cttgtcactc ataatggcaa
     5221 ttttttgacc cacgattcgg ttcaataatg tggattttcc tacattcggg cggccgacga
     5281 ttgcgacaaa ccctgattta tgttctggca tatacgttct cctttttaat taaacaacaa
     5341 atgatatatt ttagggacaa ataaaatcaa cccaatgatg acagcaaaac aagcggtcac
     5401 taaaactgct ccagctgcca tatctttgat ttttttccca atcggatgaa aatgaaaatc
     5461 cgtaaacatg tccactacat tttcaaaaac agtattgatg atctctacta tccaaacgag
     5521 aaaaacagct aaaagcagcc ataaccactc tatccgatca agctggaaaa tcacgcccat
     5581 aaataaagca agaataccga acgcaacatg tttcctcata ttgcgttctt cctcaaaaac
     5641 ggttttgact ccttggacag caaattccac agacatcagg aaatgtttgt ttttctctac
     5701 gtttttatct tttgagtcca taggcatcca agatctcttt ctgtaaacca aacatttctt
     5761 tctcatcttc tggtgtcata tggtcatacc cgttgatatg caagaatcca tgtaccgcta
     5821 aaaagccaag ttcccgatca tatgaatggc cgtaagcttc tgcttgttca gcagcacgtt
     5881 caatagagat catgatgtct cctagtacac gaggtaatgg attctctcca tcttcaaaaa
     5941 tcactgggat ttcgtcttcg ccttcttctt ccagagcaaa actgatgacg tctgtcggtg
     6001 catcttttcc acggtaatca cgattgatta cttggattgc cgcattgtcc atgaaagtaa
     6061 cagacatttc tgtttcctct ggcagcttta aataagatgc tgcaaattgc agcaagtcat
     6121 cgatttcttt cattttctct tctgagactt ggtttgtttc atccataaag gtaatatcca
     6181 ttatacatcg cttctttcta ttcttcttgt tcttccttta tcttctcaac ttctgttttc
     6241 attttaggat atttgatccg agaatggaag gtgctactta aaccgctgat taatgatttt
     6301 tccacttttc ggatttcttt caaggtcagt ccactatcgt ctaattgacc atcggaaata
     6361 cgctcttcga taagattatg gacaaaactt tcgatttttt cgctcgtcgg atgatccatt
     6421 gcccgtactg cagcttcaca actgtcagca atattgacga tccctgcttc acgactttgc
     6481 ggttttggtc caggataacg gaattgctct tctgttactt ccggattgcg ttctttcgct
     6541 ttgatgtaga aataacgcat tagcgtggtt ccatgatgct gtttgcaaat atcgatcacc
     6601 atttgcggca tattataatc ttccaatatt ttggctccat ctgtcacatg tccaaagata
     6661 atatgtttac tgtcttctgg caataagaaa ttgtgcgggt tttccgctcc tgaaggcaag
     6721 ttttcaacga aaaagctagc atgtttgatc ttgccgatat catgataata gcaagctact
     6781 cgagttagca aagaacgtcc gccgatttct gctacagcgt tagcacttag attagctacc
     6841 atcatactat gatgataagt acctggcgct tcttccagca actgtttcaa taaaggatga
     6901 tttggattgc tgagttcatt taatacaatt acactgtcat cattgaccat caattcaata
     6961 tatgggtgta gaccgatcgt cagcaagaat gagaagagtg caccagtgaa cccgcagacg
     7021 agcattaacc aagtcgttgc atcaccaaag ctcattcctt gatagacgat caaaatgaca
     7081 tccatcatca caggaaaaat aatgacccac atggcagcag ataatccttg ttctgagatc
     7141 cgtttgcgtc tgacaatcgt tgccatgagc ccagagaaaa cataagacat caagataatc
     7201 gttagaaagt tcgtgccaat cgaatcataa aaaatgaaaa gtgcggatac tgcttggaaa
     7261 ataccagcca tgatccctgc tcttcgattc aagaaaaagc ttaaaacaag tggtgcaaaa
     7321 gcagcgggat aaaacagcgg gatataagta gcttgttccg tttggaataa ttggaaaaat
     7381 ttcatcagca ctatgctgac tgacatagct gtcacataga aaagcacgta ttttgttctt
     7441 tctcctgcat tttggaattg aagtgaatta taaatcagta cgccgatttg aagcaaaaca
     7501 gctaaaacca tggcaattag cggaaaaatc gaagttgttt gattagtcag tcctagtaat
     7561 tccaattttt tcatagctga agcatcgatt tgattacctt cacgaacaat gatttctcct
     7621 tggaagatca tcactggctg tacagcttct cgtgcggatt gtttcaattc ttctgtcttt
     7681 ttctcattca atacgtcatt cactacgata ccttgatcca ctaagtagcg aatcatttgt
     7741 tgttgtgctg gtgtgacatt caaatattgg atttgctctt ctgcttcttg tttaaaagct
     7801 tccaattcac tttcacgaac ttgtttgctc atctgttcgt caatgagctt cagactctca
     7861 tccctcacag tatgaatctc agtttcagtc atttcaaata tcgtcgaata aaaattgttc
     7921 ggtagttttt gataaaacac aacattttct gcattcacat tttcaaaatt tttcttcagc
     7981 gcggcgatcc gctcttctgt agtaggcttt gggatatttt catcttcctt tgccttatca
     8041 atcttttcgt cataagattt gttgatggca tcattcgtct tatcgatcaa ttcaaatagt
     8101 tgcttgatcc gattgttctg gtcatctgca agatcttttt ggtaagtgta ttctggagtg
     8161 actgcttctg ctgccagttt tcttttttgt tcggtttctt ccgtgttctc gatcgttttg
     8221 ttcgcacgga tactttcttc tgctacttgg ccttctctga aatcattgac cttttgtttg
     8281 acactactaa atgtaacgcc gcagataatg ataaaaaaca agaacagcag gaaaggcaat
     8341 gctgcctttc caagtttttc ttgtagtttc tgcagtgttt tattcaacat gtgcttatcc
     8401 tcctaagcaa agcttattct tgatggagat ctgccttttc ataagctttg atgatttcgg
     8461 caactacagg gtgccgaaca acgtcacttg cttcaaaatt aacaaaatcg attttcttga
     8521 tttgctttaa tgttcgttct gcatgaacaa gaccacttgt cgtccctcga ggcaagtcta
     8581 tctgacttgt atccccattg acaatcattt ttgattgata accgagacgg gtcaagaaca
     8641 ttttcatctg agcgactgtc gtgttttgtg cttcgtccaa gataacgaat gcatcatcca
     8701 atgttcgccc ccgcatgtaa gctaaaggtg cgatttcgat tacgcctcgt tccatcaatc
     8761 ggttcgtatg atccaatcca aaaatttggt ataaagcatc ataaacaggc cgcagataag
     8821 gatccacttt ttcttttaga tccccgggaa gaaagcctag attttcacct gcttccacag
     8881 caggccgcgt caaaatgatt ttttgcactt cacctttttt caaggcagcg attgctaata
     8941 ctactgcaag aaaagttttt cctgtcccgg ctggtcctac gccaaatacg atatcatgtt
     9001 tggctaccga ttcgacatat ttcttttggc cgctgttttt tacccgtata ggttttccat
     9061 tacggtcttt gacgatctct tcctcataca tctcaacaaa ataatctaat gtaccttttt
     9121 gccccatttt caatgccgtc acaacatcgg gcgtacttac gtggattcct cgtttgatca
     9181 attcctgtaa tgtccgaatg actgaggcag ccaaggaaat ctgggattgc tcaccaatga
     9241 tctgaatgat ttctccacgt gtatggatgg ttgtttccgt tgtttcctca atcagtttta
     9301 tatgcttgtc gtgtgcacct aaaagcatgc tgacatcatc agcagccgtc aatctaatct
     9361 ctaaagaact agactcttcc gtcaaaaata gacatcccct ttttttcgat taattctttc
     9421 ctattttacc atactcagaa gaaactatat agcaaataag aaaaggttct aaaagagttg
     9481 cttaaccata agtaccaaga aacgttaaaa aactagccgc ttccattctc taaaggtctc
     9541 gtttgaataa aaaaagctga tcatgtaagc tatgcataag atagaggaca agtattccgt
     9601 ctaacttagc aagtattctc tgggtatttt tcttgtttca aaaaaagaaa acaaaatatt
     9661 tgaaatattc ttgcatttca aaaaaaacag gtgtattata acgtcaacaa ataaatatac
     9721 agcaaagcgt cgaggaaaac agtaggctat tcagtgattt attcccagtg agtcatgtaa
     9781 gtgagaaatg acaataaatc gaatagacga acatcatttc cgagtcaatg tcgaaaacgt
     9841 agatcattgt ggctgcctcc attatcaggc tagcggataa ctttatcgaa aagcgtccgt
     9901 gaatgaagaa gcttttttaa gcgaaaaaag gtggtaccgt tctagaatct agagcccttt
     9961 tcccaagtgg aaaagggctt tttttatatt ttaaggtggt ggttttcatg gaatctgata
    10021 ttagtggtat ttctagtttc tgcaacgttt attttgttac tcaaaatgat aattattgga
    10081 ggaaaaattt atggaagaaa caaaaactta ttcatggaag caattattga tggtcagttc
    10141 acttatcttc gggatgttct tcgggtcagg taatctgatc tttcctgtac acttaggtca
    10201 aatggctgga tcaaactgga tcagcgctgg tgtaggattt gccatctcag gcgctctttt
    10261 cccattatta gctatcttag ctgtagtagt gacaaaaagt gatggtctgt atgacttagc
    10321 taaacctgtc ggaaaatggt atgctgcctt gttcttgatc cttgttcatt taacgatcgg
    10381 accattcttt ggtacaccgc ggacagcagc aactgctttt gaaatggctg ctaaaccctt
    10441 tttgcctgaa aaatatgatt cattaggtat gtttttattc tctgctcttt tctttttact
    10501 tgcttatttc cttactgtac atccaacacg tttggtcaaa tatgtcggaa agtatttaaa
    10561 ctccttgttt cttgcattat tagctatcat ttttgtcgtt tctttattcc aacccatggg
    10621 gaatctaaac caatctgctg cagattctta tcagacgaac gctttgacaa atggcgtttt
    10681 agaaggatac aacacggttg atgcagtagc tttacttgct ttaagtgtca cttttgtcca
    10741 tgcagttcgt gatcttggtt ttaaagataa aaaagtcaca gaattaacag caaaagctgg
    10801 tagcctgagc gttgcgttag aagtcatgat ttattttggt ctggtccttc tcggtgcgat
    10861 gagtttgaat aagatgtctt tatcagaaaa tggcggaact gcactttctc agatcactaa
    10921 tttttattta ggtaacttcg gtgctagctt cttaggggta atggtcacat taggtgtctt
    10981 cactactgct atgggactcg ttgtttcttt tgcacaagat ttccataaac tgttccctaa
    11041 agtcagttat atgacatggc tgcggttaac gacttttgtc tcttttgtcg ttgcgaatgc
    11101 cggattggat aatatcatcc aatggtcttt accagtattg atgcttttgt atccattgtc
    11161 acttgcgtta atcttacttt ccttaacagc aaaattcttc caaaagagtc catttgtcta
    11221 tcaagtaacg atgttctttt ctgccgttcc agccgtattg gatatgttgg caaattcgcc
    11281 tgcattagtg agtcagcaaa ctgtcgtagc aagcttacta ggattttatc atcattatgt
    11341 tccttttgct gcattaggtc ttggctgggt cgtcccaacg ttgcttggtt acgctggcag
    11401 cttgctcttt tattatgtat atcgcgtatc aggatataaa caagaagcaa gtgagttacc
    11461 agaagaataa ccaaatcaaa cacaatttaa cataagagat attaaacagg aaaaaaacag
    11521 ctagaagaaa gaataagatt ctttcttcta gctgtttttt agttcttttt ttacgaacac
    11581 aaaaaactgc ggataatcac tgtccgcagt ttaaatgata aaattaagat tgtaatagtt
    11641 ctttcacaat cgcattcact tggtttccat ctgcttttcc tttgacttta ggcataattg
    11701 cacccataac tttgccaaat tctttcattg atgaagcacc agtttgagtg attgcttctt
    11761 gaacaagttg tcgaatttct tcgtcggaaa gttgttccgg catatatttt tcgactatta
    11821 caatttcact ttttactttt tcagctaaat cgtcacgtcc tgcttcttca aactcatgca
    11881 aagaatcacg tcgctgtttc atttcacgag acaacaccgt tagttcttct tcttctgtca
    11941 agtcgtgacc ttctttaatc tgctcgtttt gtatagatga cttgatcatt cggatgactt
    12001 gtaaagattc cttatctttc gctttcatcg ctgctttcat gtcatcgttt aatctactta
    12061 gtagtgacac actaccaact ccaattcatc acagaactag aattttttgc gttttctagc
    12121 tgcttcagat tttttcttgc gtcttacact tggtttttcg tagaattcac gtttacgaga
    12181 ttcttgtaaa gtacccgctt ttgacacgga acgtttgaag cgacgaagag catcgtcaag
    12241 tgattcgttt ttgcgaacga ctgtttttga catgttaatt ccctccctcc gagcttaaaa
    12301 agtaaccgta attgttattg aaatatatct caaaaaacaa gactgtcctg taaatattat
    12361 atctaatgct gttttgaccg tcaaggaaat cttgcttttt ttttaaatta tcaaatgtta
    12421 ttttatttaa atccttattg acaattttca catcttccga aaatctcgaa tctatgccct
    12481 tcgatcgtgc atccacctag ctgttcttcg aaataattca tcggacacat atggatttct
    12541 ttcgttgttc cgcacactgt acagataaaa tgatggtgat ggccgatttc cccctgacag
    12601 caatgaaaac gaaacttcat ttctccattt aaatcggttt cttccagtaa atcgatctct
    12661 gcaaaatcat ggagattccg gtaaatcgta tcataactta cccctttgaa ttcctgattc
    12721 atatagtcaa aaacttcttt agcagatacg tagcggttac gcttcactaa ataagttaac
    12781 aatgcttctc tttttttagt atatttaaaa ccgttatttt tcaatgtttc taatgattgt
    12841 tcgatcaatg tctgtttttt catgacttat tcctccaaat caaaatgatt acgaagtacg
    12901 cattctagag tatggtataa taaacataaa aaaaatgcaa gtgaggtttt atccatgact
    12961 tccgattcta cagaaatcgt tacttttttt gtcatctccg actctgctgg agaaactgcg
    13021 acaaaactag cgcaagctac tatggctcag tatcctaccg ttgagttcaa cctattccgc
    13081 agaacgttcg tgacagatga aaaaacatta aggcaggcat tgcaagatgc attagaagaa
    13141 aaagcacttg tcctgcatac attaatcaat caagaactga tcgagataac acgttctttt
    13201 tgtgaagaac atcaattatt tagttttgat gtgatgacgc caccgatatc agaaattgaa
    13261 cgtttaactg gtatcaagcc aattcggcaa cctggtgccc tccatttatt gaatgagaac
    13321 tatttcaaac ggatcaaagc aatggaattt gctgtaaaat acgatgatgg gaaagatcct
    13381 cgtggttttt tagaagcaga tgtcgttttg ctaggcgtat cccgaacttc caaaacgcct
    13441 ttgagcttgt ttttagctaa caaaaattta aaagttgcca atttaccttt gatcccacaa
    13501 gcacatattc ccaaacagct ttgggagatc gatcctaaaa aaatcgtggg actaacgaat
    13561 aacccagata ttttaaataa tatccgtaaa gaacggatgc gctcttacgg attaaaccct
    13621 gatactgctt attccgatat cgaaaaaatt agagctgaac tcgattttgc gaatgatctt
    13681 tatgaaaaac taggatgtgt agtgatcaat gtagcatctt tatctattga agaaacggct
    13741 tcgcttattt tgaatgcttt agacctagaa gatcatagtt attatggaac tgaaacaaca
    13801 gaaaaaagcg attgatcatt tttgtctttt tcttaaaaaa cactggagtg tgacaacgaa
    13861 gaaatttcgt tgtcacactc cagtgtttat ctatttgcac ctatcgttat tgatgttcca
    13921 catgtttgag tgtctggttc aaaatatcca gtacatgaga atcatcaaga ctatacaaaa
    13981 tcgcttttcc ctcgcgatga gacttgacga catgattttc tcttaataaa cgtagttgat
    14041 gcgatacagc agactgctcc atttccactg cttgggtgat tgctgtcaca ttttgttccc
    14101 cgtctttcag atatagcaga attttaagac gagtgggatc actcaacatt ttgaataatt
    14161 gactgacttt ctctactttt gatttttgcg tgtctggcaa tccattcacc tccaacttga
    14221 aaaaagactg acttctaaag tttaccactt tttccatcag tctcaagttt tttattcttc
    14281 tgtcatcttt ttgataatcg cttcataaga ggctactttt ttggctgcgt cttctgtaga
    14341 gcttcctttt acaccaatat aaaacttgat tttcggctca gttcctgaag ggcggatagc
    14401 aatccagcca ttttcttcca atgtatattt caatacgtcg gatggcggtg ttgtgagttt
    14461 ttctacgttt ccttcacggt ctgttctcgt caattcttta aagtcttctg tttgaataac
    14521 ttttgctccg gcaaattctg tcggtgcttc tttacggaat ttttccatca aagcagagat
    14581 tttcgcagaa ccttcttgtc cgctcattgt cacagaaatc gttttttctg cgaaatagcc
    14641 ataggtttca aaaatcgttt gcaagccgtc atacaacgtt ttgccttgtt ttttatagta
    14701 agctgctact tctgctaata aaagcaatgc ttggatcgcg tctttatcgc gaacaaaagg
    14761 tttaactaaa taaccataac tttcttcgaa tccaaacata aaagtatgtg aatggttttc
    14821 ttcgaattgt tggatttttt cagcaatgaa tttaaaccct gtcaacacat tgaacatggt
    14881 agcaccgtag tgttccgcaa ttgctgtagg caattcgctg gaaacgattg atttcaatac
    14941 cgctgcgttt tctggtaaag tgcctcttga ttgatgtgct tccaaaatat attgagcaaa
    15001 caatgctccg atttggttcc cagtcaatac ttcataagaa ccatctggca aacgaaccgc
    15061 tgcgcctaaa cggtctgcat caggatctgt tgcaatcagc aagtcagctc cttctttttc
    15121 tccaagtcgt atagcatatt caaaagctga atgttcttct ggatttggcg atttgaccgt
    15181 agtgaaatct ggatctgcca cagcttgttc gggaactagt acgaattgtt cgaatccagc
    15241 ttgtttcaac gcgcgttcgc ccaacatttt ccctgttcca tgaagtggcg tgtacacaag
    15301 tttcaaatct ttccccattg tttcaaccaa ttcacgatca atcgtcactg ctttgatttc
    15361 ttctagataa gcagcgtcca cttcttctcc gatgatcgtg atcaacccgc tatgtttagc
    15421 ttcttcgtca gataatactt cgacttttag cggattgctg acttcacgga catatttcgt
    15481 caatgcatct gcgtctgcag gaggcatttg tccgccatct tctccgtaga ctttatatcc
    15541 attgtaagcc gctgggttgt gagatgcggt taccatgatt cccgcaaacg tcttcaaatg
    15601 acgaactgca aaagaaagct ctggtgttgg acgcagactt tcgaatacat aagacgggat
    15661 attgtgatgt gccagtgttt tggctgcttc cattgcaaat tctggtgact gatggcgtga
    15721 gtcataagcg atagcgacac ctcggcgttt tgtttcttca ccttgtgtat ccatgaaacg
    15781 tgctagtcct tctgttgctt gtcttaccgt atagatgttc attcgattga tcccaggtcc
    15841 taagattccc cgcatgcctg cagtaccgaa ttccaacggt gcataaaaag catcttcaag
    15901 tttttcagga tcttcaccca gatcatttaa ttgttttttt aagttttctt ctaaattttc
    15961 ttcatgtacc cattgttcat acacttgttc ccaagacatg aaaataacac ctctttctga
    16021 aatatggttc ttaataagta tagcattcac ttattagata tgaaagtatg aggaccaaaa
    16081 aacgcttaaa tcaagaaagg attcttttat caagcatgat tgcaagtatt cacataaatc
    16141 ggattcagtg gatttcacgc gaaaaaaaaa gagtaaaaga acaattaaaa ccgttctttc
    16201 actcttttta aacttatgct gttgtttctt ctaaagactc caagtattta tacacttgtt
    16261 gtccagcaat accgccgtca ccaacagctg tagtgatttg tcgcaaatct ttttcacgaa
    16321 catctccgat ggcaaaaacg cctggcatat tcgttttcat ttcgttgtct gttggaatcc
    16381 agccagcttc gttcgtcaaa cctgcttctc taaaaggtgc agttaatgga tccagtccaa
    16441 catagatgaa tactccgtct gctgctaaat caaattcatt gccggttttg acattacgtg
    16501 ctttcacacc tgtcactacc atttcgtttc caacgatttc gtcaacgact gtatcccaca
    16561 taaaggaaat tttttcattt gcaaatgcac ggtcttggat gatcttttgt gcacgtaatt
    16621 catctctacg gtggacgatc actacttcag aagcaaattg tgtcaaatag atggcttctt
    16681 ctactgctga gtccccaccc ccgatgacta ctagacgttt gttacggaaa aacgcaccat
    16741 cgcagactgc acagtaagaa acgcctcgtc cagcaaattc ttcctcgcca ggtacgccta
    16801 atttacggtg gatacagcct gttgcgataa tgactgtttt tccttcgtaa cttttatctt
    16861 cacagatgac ttctttatgg ctgccgtgat ccttgatatc ttgaacgatt ccataagcat
    16921 tttctgtccc aaatcttgag acactttcat acatcttata agctagatcg gggcccatga
    16981 tcgaatcgaa tccaggataa ttttcgattt ctgccgtatt gttcatttgg ccgccagggg
    17041 caccttgttc gatcatcaat acggataagt ttgaacgtga agcatataaa gcagcagtca
    17101 tcccagcagg acctgcgcca ataacgatta catcatacat atgtagattc tcctttactt
    17161 aatcaagtaa gcatacttta cagccaatgc gaaaaaaagt ctattgttct gcttgcgttt
    17221 ctcgaatagc tttattatac tcgaaaaatc cgctgaaaaa caatagactt ttataaatca
    17281 ttgatcaatc gttcgaaaga ttttctccat ttgaatcaat tactttttta taccagttga
    17341 atgatttttt ctttgaacgg gctaatgtgc catttccttg gttatcacga tcgacataaa
    17401 tgaatccgta ccttttcttc atctcaccgg taccagctga tactaaatca atacatcccc
    17461 aagtcgtata gcctaataga tcgactccgt ctagttctac tgcatccttc atcgcctgga
    17521 tatgcgctgc caggtaatcg attcggtaat catcttccac ataaccattt tcatctggcg
    17581 tatcgactgc acctaagccg ttttctacaa taaataatgg tttttgataa cgatcataaa
    17641 tatcattcat cgttattctc aagcctaatg gatcaatttg ccacccccat tcacttgctt
    17701 gcagatatgg atttttgaca gaagcaaaga tatttcctgc agtctgttcc aagagttcgt
    17761 tgtcggtagt cgaaactcgg gaagaataat aagaaaagga gataaaatca acagtatgtt
    17821 ctttcagcaa tgcggcatcc cctggttcca tttcgatctg aatgccattt cgttccattt
    17881 ctttcaatgc ataagctggg tattctcctc gtgactgaac atcaatgaag aaataatttt
    17941 ctcgatctga ttttctagaa tcccaaacat cttcaggatt gcaagtgtat ggataatatg
    18001 ctcctgctgc cagcatacag cctactttgt tctccggatc tacttcatga gcaatcttcg
    18061 ttgcgatggc acttgcaacc aactcatgat gtgccgcttg atatttgatt tgttcttcat
    18121 tttctccttt ttcaaaatac agccctgcgc ccatgaatgg tgcatgaagg atcatattga
    18181 tctcattgaa tgtcagccaa tatttgacaa gtcctttata gcgattgaag atgactcgac
    18241 acagattttc ataaaaacca acgagttctc tggaacgcca cgcaccatat tctttgatca
    18301 aatgcattgg acaatcaaaa tgcgtaatcg tcacaagtgg ctcaattccg tgcttttggc
    18361 attctttaaa gacatcctca taaaatttca agccagcttc attcggctct ttctcatccc
    18421 ccattggaaa aatacgactc caggcgatgg acattcgata agtcttgaac cccatttctg
    18481 caaacaaagc aatatcttct ttataacgat gatacatatc gattgcaatt tttgctggat
    18541 aaaaatggtc ctcatcaaat tcaaacattt ttttctttcc ggtgataaca ggaaaacggt
    18601 cttttccgat tggcacaaca tctacgtttg ctagtccgcg tccaccttca tcataaccgc
    18661 cctcacattg attggctgct gtagcaccgc cccataaaaa atcttttctg aatcccatta
    18721 tacttcctcc attatatgtg tattacttga taattctctt gccagttcat cttgaacttc
    18781 gattccttct tcttttaatc ggccgattgt ttccttgaat tgcggtaaat actgctggtg
    18841 tgcaataaac aactcatcca tgatccgttt tgctgtcttt cctgatcgta taagtggatt
    18901 gatcgtaaac gcctgtaagg ctgtcccata atcaccagtc acagctgctt cgatcgtcag
    18961 caattccata ttcttcatta cctgcaacca gcctctttca gcagcaggaa gagaaccaaa
    19021 cgctacattt ctcgctcctt gtgcgccaac ataggcagtg acttctacaa cacaatctgc
    19081 cgacaggtca ggaacagctc catcattctt tgtagataca acgatctgtg tttctttatt
    19141 tccatagatt gaagtaatcg tttcacaggc agcgtctgaa taataagcgc cacctcgttt
    19201 ttgcaattgt tcaggtttat ggtctaattc tggatctttg tataattcaa acagctctgc
    19261 ttctgtttct ttcacttgct gcgctcttgt accaatcgtt tgatattctt ctaaagcatg
    19321 ttccagcatt tcttcttcac gatagtaata gcggtggtag ccacaaggga tcatgttcat
    19381 ttgttttaat tgttctttga aaaacgggat atcaaagata tttttcggca atccattgtc
    19441 ttcatcgtat aaacgatcaa tcaattctgc cgtgcggtca tttcctttat tatctgctac
    19501 tttgtgccaa tggaaatgat ttagaccggc aaacttgtag atcagctctt cttttttctt
    19561 gccaagcatt gctggttctg tcatcatcgc cattactggc acattgcata atccaatcac
    19621 tttatcccat ttaccatatc tgataactgc ttctgtcacc atcccacttg ggttcgcgaa
    19681 attgatcagc caagcatttg gacataaacg cttcatgtct tcaacgattg ccaagaccac
    19741 tggcacggta cgaaaagctt tgaatatccc gccagcacca tttgtttctt gtccgatcat
    19801 cccgtaataa ctagggatcc gctcgtcttt gattcgtgca tctaattgac cgacacgaaa
    19861 ctgagtcgta acaaaatccg catctttcaa tgcttcttcc cgatccagag ttgcatagat
    19921 cttgacttca tacggagaag catcccacat acgctgagac atctgactaa cgatatcaag
    19981 cttttctttt ccttcttcta catctacgag ccagatttct cgtatcggta attcctcata
    20041 tcttttgata aatccttcca tcaactcggg ggtgtaactg ctgcctccac caatcgtagc
    20101 gattttgatt ccttttgcca tgttctcact cctttacttt tttacacttt aattatcttt
    20161 tttgtaaaca aaacaaacaa tagaaatccg tttctttcca gtaacattta caaaagttac
    20221 tgttttctag tggatttttg taaatatctt gtcaaaaaat gtctatttta agttatatag
    20281 tataaatccg tttacaaatc attttcaaaa aagttataat ttacgtaaaa aaataagaag
    20341 aaggtatcta catgttatta cgtgaaaaaa tcaaagaaat ctccttttct ccttcagaaa
    20401 atgaagtgat ccagtttctc ttaagcgatt ctccagcact tggcgaaatg accatccaag
    20461 agatcgctga gcgatgttat gtccacccgt ccacactcat ccgtgtagca aaaaaaatgg
    20521 ggtttgatgg ctgggtcagt tttcgtaatg cttttttagc tgaaacagcc tatcttgaag
    20581 ggaattttca agacgtagat gcgaatcttc ctttttccac cacagacggt attatgacga
    20641 tcgcaaataa aatggcttct ttagaacaga tgacgattca agatacactt tctctcattc
    20701 atcatgacca actgcaaaaa gctaagtatt tactgctcca ggcaaaaaaa atcgtgatct
    20761 tcgccagtaa ctcgaatact ttgatttctc aagatttcat gctgaaaatg aagcgcatca
    20821 aaatggatgt cacaattgtc accacttttg gtgaatcatt ctatgaagcg tacaattgca
    20881 cgagcgatat gtgcgggatt ctgatttctt atacaggtga aaacaatgtg atccttcgta
    20941 ccgcagaaat attgaaacaa aatgatgttc caattttagc tttgaccagt atcggtgatt
    21001 caacgctatc tgcaatgagt acagccgctt tgcgacttac gacacgcgag cgactgtatt
    21061 caaaaattgg taattttacg atcaatacat caatttgtta tcttctagac gtgttatata
    21121 gttgtgtttt tgcagaaaat tatcagcaga atttagtcca tctaatttct gtcggtcaaa
    21181 aattcgataa acgccctatc agctcagcca tcatgaaaga gcctttcaat tttgaaaata
    21241 aagaagtgga ataaaaagaa tagttaagcc tagtccattc ttcacctaac cgttcaggtc
    21301 agtgcggtga taagtgcatc gtctggcccg accgtctccc cttggttttc caaaatatcc
    21361 aaataatccg tataattggt gacgatcaca ggtgtttcta aactgtatcc ttccttttcg
    21421 attttctttt tgtcgaagcg tagcaacagg tcgccttgtt tcactctttc tccttgctgc
    21481 actaagcttt caaaatattt cccttccaac tgcacagtat ccaatccgac atgaatcagc
    21541 aattccagcc ctgtttcaga aaccattccg attgcatgtt tcgtcgggaa cagggtcata
    21601 actgttccgt caaaaggggc acgtacttct cctttttcag ggtagatcag gatccctcga
    21661 cctaatgttc cttgagcaaa agctgcatcc ttcgctgcat ttagtggagc gatttgtcct
    21721 tgtatcggtg tttttacaat ttctttaccc gatcttttct gtatatcatc tgtttcacct
    21781 gcttcatcct tccaaaagaa aaaagtcagc gcaaaggata ccaccatcgc aatggcgatc
    21841 ccaattaata cattaatcac atctgttaaa tcaccatctg gtctaatata gttgggaatc
    21901 gtaaagatgc ctagtccacc ttgattccaa gaagttaaat tcattaaccc actatacgcg
    21961 ccccctaacg caccaccgac acaggtaaaa ataaatattt ttttacgtgg caatgtaaac
    22021 ccataaatag ctggctctgt aatccccaca ataccagaaa caatggctgg cattgcgatt
    22081 gctttgtctt ttgggtttct aagcttaaag tagagtgcag taagtgctgc agtttgtcca
    22141 aaagacacta ctgcaacagg tgtcatgata ttttgccagc cattcacaga aaattgcatc
    22201 aatcccattg gtacgattgc ccaatggaga ccaaacatca ccagaatttg ccagaagaag
    22261 cctacgattg ctccataatt aactggcgaa agtgctaaaa gtgtttcaaa tccagccatt
    22321 aaaatatcag ttgcgaatgt gaatgctggt ccaacgagta aaaaacctaa gggcattgta
    22381 ataagaacgg tgaagaatgg aacgaagaaa ttcgcgatca tctcaggtac taacttttta
    22441 attggcttct gaatttggct agctaacaac acaataaaga taatgggaat gacagaagaa
    22501 gcgtagttac gcgcaatcca gggaatgcca aatactgttt gataaatagg tgcttcaaaa
    22561 atagtaccag aaaatatggt cgtcagtggt gcttctgctg caccactcaa aacttccagc
    22621 gacaagttag gattcattaa tgctgcgcca agcaacatcc ctacgaattc atttacttta
    22681 aatttacggg caactgttaa accgataata acaggcataa attggaatac agaatcaccg
    22741 atattgtcga acataacaaa tgttccagaa ccgcgatcaa acgcgcctaa gaagacaagt
    22801 aatgctgtca gtccacgaat catcccacca gcagccagga tagcaaggaa tggctggaaa
    22861 catcccgata ttccatcaat aagcatatca aacggtctca ttttttctgt tgaaacagcc
    22921 tcatcaaaag taaggtttcc tgcttttata acctcttcaa acacgactcg cacatgattc
    22981 ccgatgacaa cttggtactg tcctcccgct cgcattaccg tcacaatacc gtccatattt
    23041 ttcaagatct catcatttgc tttttcttcg ttttttaatt tgaaacgaag ccttgtgata
    23101 caatgtgtca aaccattgat attttccttc ccgccgacat tttctacaat tttgctcgct
    23161 aattcttggt attttcccat ttctgttccc tccaattaat tagtggaagg tttagccaac
    23221 tgaatgtcac tacccttatt tcatacgttc gtgttaattt gtcctccttt ttatttattt
    23281 acaacgattc tcatgcacta aacgcgcagt atggatcgtt aaatacattt tttcatccgt
    23341 ggacatctgg tagtcgaaag ccccggttaa aaagtctgct attttctcaa cgcaaccaaa
    23401 cgtatcctta tatttttttt gaataacttg gaacaattcc tcagaaactt ctccatcttc
    23461 tgatttcgct ccatctagaa cacgtgtcag aaaaaaacgt aggtgtgtga caaatcgaaa
    23521 aaaatggact gaatcttcat caaaagtaat cttatagtaa cagcggacga tatttaaaat
    23581 atcctgtatt gttgtggtaa acaggtaggt attgtcttgc tggctttgaa tgccttgtgc
    23641 attaactaaa tgcaatgcga taaatccggc tttgtcttct gataacttga tgtggtattt
    23701 atccgagatt ttcttcactg tttttttccc aatggcatac tctttaggaa acagttgttt
    23761 gatatcatac agcaatacat ttggcacaaa gatccccatt ttttgacgtt cgatagctgt
    23821 atacaaatga tccgtcaatg caatatacac agaatcattc aaatggactt gtagatcctc
    23881 ttttgtatat tcaatgattt cacaagaaat ttctaagtac tcaagaggaa tcgcttgtgc
    23941 caattcttgg aactgttgtg aaagtccgtc atttgtcaac tgaaattttt tgtcgatgca
    24001 tgagtcaaaa atcgggtcac ccactttctt tttgaatgcc aaaccttttc ccatcacgat
    24061 catttcctga tttctttcat ccctggttat tacgacgttg ttattcagta ctttctcaat
    24121 cagcatgttt tgctcctttc catgatatat agggctgtat ccaaaacaaa aagaaaaacc
    24181 caaaaagccg tccatgaaac catgggagac tcatcaggtt tagcttgcat taggcaatca
    24241 ctatccaagt tgtatcgttt tctttgtatt atcgaaagca aacgattttg tcaacgcttt
    24301 cttaggggct actaggcact tctgactagc tggcgtcgtc ttttttaaat ctggtaacta
    24361 cgacactaac tctcacagat gaaaaatatg ggaagctatg ttttattttc actctagtgc
    24421 ttaggtcaaa atgctactgt cacaacatct tttatatacc actgctttgc agtttgtaca
    24481 tatctgcata caaaccattt tgcgctagaa gtgtttcatg attcccgtgt tctactatat
    24541 gacctttgtc taaaactaaa atcaattctg catctcgaat cgttgacagc cgatgggcaa
    24601 tcgcaatcgt tgtccgtcct tgtcgcattt tcgccaatcc ttcttggatc aatccttctg
    24661 tttcagtatc gatatttgct gttgcttcat ccaatactag aattttagga tcagtcacga
    24721 ttgttcgcgc aaaagtgatc agctgtcgtt gtccactaga atagctggca ccatgttcga
    24781 tcacttttga gtgatatcct tttggtaatt catcgatgaa tttgtttgct tgaacaaact
    24841 cagccgcatg gcgtatctgt tcgtctgtga tagctggatt catcatccga atatttcctg
    24901 cgatatcccc ataaaacaag aaagcatctt gcaagaccag ccccattttc ttacgtaact
    24961 cttccattgg atattctcga atgtcatgat catcaatcaa tatctgacct tcataaaatt
    25021 cgtaaaaccg catcaacaca ttgatgatcg agcttttccc acttccggtg tgtcccacta
    25081 atgcaacggt ctctcccgga ttagcaacaa aagagatatt gtttaataca ttgtgctctc
    25141 catcataaga aaagcttaca ttgcgaaact caatcttacc tgaagtaatc atttgatctg
    25201 ctccagggtt ttgttgtggt gcatattctt ctgtatccat aatcttcaag atacgactac
    25261 cagcaaccat cccatctgtg aagatgctca aaaagtccat catttgagac attggattaa
    25321 aaaatccctg gacataggtc gtaaatgcat agatcagtcc aacttctatg ggagaatgca
    25381 acgcatcgat cccaaacaaa gtcaacacca acgcaatcgc cagtgcatat agaaaattga
    25441 tgatcggact taatagaatc gaattcgttt tcatcatcgc ataacgcgtg tctaaatact
    25501 cattgttcac ttgttcaaat tctttagcta aacgagcttc ttggcgaaac tgctggataa
    25561 tacgcatgcc ggaaatcgat tcattcaatt tcgtattcag ctgacttaat ttttctcgca
    25621 tattccggta aatacgagaa ctaaatttct ggtaatacca gatgacgatt cctaaaattg
    25681 gcaagaacac caaagtccac aaggcaattt ctccattgat ttgaaacatg gcaatgaatg
    25741 aagaaacgac tgcaaagatc ccagtcaata ccatcaaaaa gacgtaccaa aattcaaata
    25801 gcgtttctgt gtcactcgtc actcgagaaa cggttgaacc tgccggtgtc tgatcaaaat
    25861 aacgcatacc tagtgtatgc agcttttcaa ataattttac cctaatatac tgataggtct
    25921 tcaatgaagc catggaatac aaataccatt ggaaaaacca aatgatactt tttactatga
    25981 ctccaaataa gtacatacca gcaaaaaaca aaataacttg tgtcgtagcc gactgtttag
    26041 ccagatgatc atccataaaa gtttggatga tccttggcaa taggacattg atcacagaca
    26101 gagcaaacgc aaataagata gcaacgatga acgttcttct aaaaggtttg gcgaacttca
    26161 tcagtcgtgt gatgatcttt ttctgttctt taagtggaat cgattttgtc cattccgatt
    26221 gataattttc atccatcagg cctcgcctcc ttctatcttc gcttctaatt gctgtttctc
    26281 ccacatttgt tcataccagc cgcctagtgc aagcagctca tgatgggttc ctcgttcaat
    26341 gattttccct tgatcgacaa caatgatttc ttttgcatgc attacgctac ttaaacggtg
    26401 agctgcaatg atagttgttt tctcttttct tatttctttt aaattatgca agatagcttc
    26461 ttctgttttg gcatctactg cagaaagtgc atcatctaac attaataatt caggattttt
    26521 gatcaatgct cgagcaatcg aaatcctttg tttctgtccg ccagataaag agactccacg
    26581 ttctccaact aaggtatcat aaccttttgg taattccttg atttcattgt tgataaatgc
    26641 taaagaagcc gcatgctcta cttcttcttc tgaaagtttt ggattagaaa aacggatatt
    26701 atctcgaatg gtcatcgaaa acaagaagtg gtcctgaggc acatagccga ttgatcctaa
    26761 taaagcattc aatgaatatt ctttgatcga acgtccgcca aattggattt tcccttgata
    26821 atcatcgtat tcacgcatca gtaatttgat gatcgtcgtt tttcccgcac cagttttccc
    26881 aacaatacct aatgtttctc cttcttggat cgcaaacttg atttgttcta atgctggtga
    26941 ttcttctttc ggataattaa aagaggaaat agctacattc agctcgcctt ttgccggggt
    27001 ctgcactgca cccagttgtt ccacgatatg cgtcttttca tgcaaaagtt cattgacgcg
    27061 atcataagaa gcatttcctc tttccaatac gttgaacaat cgaccaatcg caaacatcgg
    27121 ccaaacaagc ataccgatat aactaataaa tgagaccatc tgcccgatcg agatcgtgcc
    27181 ttccaagaca tactttccac ctaaaataat tgttgcaaca taagacagac cgataatcaa
    27241 ggtaatgaat ggatcaaata aggagtctaa aaaattgact cgtttgtttt taataattgc
    27301 atcttcaatc ttttcttcaa aatccgctag atcttctttt tcttgtccaa atgttttaat
    27361 gactttgatc ccagtgatac tttcttgtgt cttatcattg atactagaaa aagcttcttg
    27421 cgaatcacga aaagcatcat ggagttttga ccccagcact cgtgaagtga cggccagcaa
    27481 aggcagagga atcagcgcaa tcagtgttaa gcgccaatca ataaacaaaa tcatcgcaat
    27541 gatcgttgtt ccgcccgtaa tcactgaatc tgcaaaggtc aagataccag ctcctgctac
    27601 attctggatc gcattcaaat cattcgttgc gtgagccatc aaatccccag tccgatattt
    27661 ttggtaaaag agattgtcca tttctgtaaa atgtttgaat aactgctgcc gaagttcttt
    27721 ctccaatcga gctgcacttc cccaaatatt cgtccgccag atataacgaa aaagatattg
    27781 taaaaaagca gcgataatca ataggaagat ccacccgaaa atgatcttta ttgataaatt
    27841 tttatcggcg atctcatcaa tgatgatccc aatcactttt ggaggaacta actgtaccaa
    27901 ggccactgcg aatagcgaga aaaccccaat taaataatgt ttcttttcct gacgaaaaaa
    27961 ccagcctaat tttctaaaaa tcgacatttt actcctcctt tgtctttatc aagagcctag
    28021 gattgtctgt gcttgtcttt tttgctgaat aatctgaata gtctaacaaa ttacgaaata
    28081 aaaaacggac tgaaaaaatc agcccgtaaa aagtcgttgg gtcagaacct attttttccc
    28141 ttggttcttc atttgctgca tcatctggcg aattttcttc tcagatggtt tttgccccat
    28201 tgacatcatc attgatcgca acatgtcctc attaacggga ggattttttt ctaaatagtc
    28261 tttcatatat ttacgcgcta ggaaaaagcc acctactgcg ccgattagca aagcaataat
    28321 agcaataagt acgacaatac ctgttgataa catataaatc tctcctttct atttccgaat
    28381 tgctctcaat gacttatttt actaaagttt attgaaaaaa gaaaggcgta ttctttagtt
    28441 tcatgcaaaa aatagtggtt tttttagagg agaacaaaat ttctttcgtc atctagcaag
    28501 acgcgccatg aaaaaaaatt aggcgtaccg aatctttttc tttaattttt tctttttgag
    28561 gtgtagaatg taatcgtaga taaataggaa aggtggtttt ttcttgaata atcaatccaa
    28621 taaacaattt cataagcatc gacttattga acatacaaat ggtttatctt tagctgaagt
    28681 aaatggaaca gttgatgtac caaaggggaa aagttttttt aaaacacttc ttgcttactc
    28741 tggacctggt gcgttagtag cagttgggta tatggacccg ggtaactggt ctacttcgat
    28801 caccggagga caaaatttcc aatatttatt gatgtcagtt attttgatgt ctagtctgat
    28861 tgctatgctt ttacagtata tggcagcaaa attaggaatc gtgagccaaa tggatttggc
    28921 acaggcaatc cgagcacgca caagtaagac attaggtgtc attttatgga tcttgactga
    28981 attagccatt atggcaacag atattgcaga agtcattggt gcagcaatcg ccctctactt
    29041 actttttgat attccattgg tgattgccgt ctttattact gtattagatg ttttcgttct
    29101 cttactactg acaaaagtcg ggtttcgtaa gatcgaagca ctggttgtct gcttaatttt
    29161 cgtcatttta ttcgtttttg tctatcaagt tggcttgtct aacccagatt ggcacagttt
    29221 atttgctggc tttatcccta acagtcatac attctcagac tcaccggtca tcggcggcga
    29281 aactccgctg actggagctt tagggattat tggtgcaaca gtcatgccgc ataacttata
    29341 tcttcattca gcagtttcac aaactcggaa gatcgatcac caaaacgaag aagatgtagc
    29401 acaagctgta cgtttttcta cttgggattc taatatccag ttgtcaatgg cttttctagt
    29461 caatgcactg ctattgatca tgggtgtcgc tgtcttcaaa accggttctg tccaagatcc
    29521 ttcctttttt ggtctgtatg aagctttatc tgatccatca gctatgagca atggtatttt
    29581 gattgctgta gcaaaatctg gtatcttgtc cactttattt gctgtagcac ttcttgcttc
    29641 tggccagaac tctactatta ctggtacgtt gaccggtcaa gtcatcatgg aaggattcat
    29701 ccatatgcgt atcccgattt ggctaagaag actggtcaca cgattgcttt ctgtgattcc
    29761 tgtattgatt tgtgttttgt ttacaagtac aaaaggaacg atcgaagaac atgccgcttt
    29821 gaataactta atgaatgaat cccaggtgtt cttggctttt gcgttgccgt tttcgatgat
    29881 tcctttactg atgatgacca acagccaagc ggagatgggt agccgattta aaaattcatt
    29941 catcgtaaaa cttcttggct ggttctctgt gattagtcta acttacttga acttacgagg
    30001 attaccagga cagatcgaaa gtttcttcgg cgagcaagcc acttccgctc aactagcatt
    30061 ggctgatcac atcgcttatg taatcattgt tcttgtcctt ctcctgttgg catggacgat
    30121 tggtgattta tataaaggaa acaaaaaata tgaacagctt ttgcaggaaa cggtagaaga
    30181 aatcgaataa aaactgattt ttccacacaa agacccttga tagttaggtt aaaaat
//
LOCUS       DAZABD010000033        29107 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000033 DAZABD010000000
VERSION     DAZABD010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29107
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.033"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1030)
                     /locus_tag="ACHALG_001536"
     CDS             complement(<1..1030)
                     /locus_tag="ACHALG_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFN2586937.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1112..1801)
                     /locus_tag="ACHALG_001537"
     CDS             complement(1112..1801)
                     /locus_tag="ACHALG_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFN2586938.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1907..2275)
                     /gene="rplL"
                     /locus_tag="ACHALG_001538"
     CDS             complement(1907..2275)
                     /gene="rplL"
                     /locus_tag="ACHALG_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFN2586939.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2328..2831)
                     /gene="rplJ"
                     /locus_tag="ACHALG_001539"
     CDS             complement(2328..2831)
                     /gene="rplJ"
                     /locus_tag="ACHALG_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFN2586940.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(3035..3162)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(3241..3930)
                     /gene="rplA"
                     /locus_tag="ACHALG_001540"
     CDS             complement(3241..3930)
                     /gene="rplA"
                     /locus_tag="ACHALG_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFN2586941.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4034..4456)
                     /gene="rplK"
                     /locus_tag="ACHALG_001541"
     CDS             complement(4034..4456)
                     /gene="rplK"
                     /locus_tag="ACHALG_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFN2586942.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4799..5467
                     /gene="sdaAB"
                     /locus_tag="ACHALG_001542"
     CDS             4799..5467
                     /gene="sdaAB"
                     /locus_tag="ACHALG_001542"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HFN2586943.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5480..6352
                     /gene="sdaAA"
                     /locus_tag="ACHALG_001543"
     CDS             5480..6352
                     /gene="sdaAA"
                     /locus_tag="ACHALG_001543"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HFN2586944.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6443..7543)
                     /locus_tag="ACHALG_001544"
                     /pseudo
     CDS             complement(6443..7543)
                     /locus_tag="ACHALG_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324221.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
     gene            complement(7635..8522)
                     /locus_tag="ACHALG_001545"
     CDS             complement(7635..8522)
                     /locus_tag="ACHALG_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFN2586945.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(8599..9144)
                     /gene="nusG"
                     /locus_tag="ACHALG_001546"
     CDS             complement(8599..9144)
                     /gene="nusG"
                     /locus_tag="ACHALG_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFN2586946.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9245..9415)
                     /gene="secE"
                     /locus_tag="ACHALG_001547"
     CDS             complement(9245..9415)
                     /gene="secE"
                     /locus_tag="ACHALG_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFN2586947.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9439..9591)
                     /gene="rpmG"
                     /locus_tag="ACHALG_001548"
     CDS             complement(9439..9591)
                     /gene="rpmG"
                     /locus_tag="ACHALG_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFN2586948.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9753..10397
                     /gene="cbpA"
                     /locus_tag="ACHALG_001549"
     CDS             9753..10397
                     /gene="cbpA"
                     /locus_tag="ACHALG_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-AMP binding protein CbpA"
                     /protein_id="HFN2586949.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10453..10956)
                     /locus_tag="ACHALG_001550"
     CDS             complement(10453..10956)
                     /locus_tag="ACHALG_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFN2586950.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10969..11015)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11553..12518
                     /locus_tag="ACHALG_001551"
     CDS             11553..12518
                     /locus_tag="ACHALG_001551"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFN2586951.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12617..13789)
                     /locus_tag="ACHALG_001552"
     CDS             complement(12617..13789)
                     /locus_tag="ACHALG_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN2586952.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13776..14921)
                     /locus_tag="ACHALG_001553"
     CDS             complement(13776..14921)
                     /locus_tag="ACHALG_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN2586953.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14935..15867)
                     /locus_tag="ACHALG_001554"
     CDS             complement(14935..15867)
                     /locus_tag="ACHALG_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN2586954.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15984..17063
                     /locus_tag="ACHALG_001555"
     CDS             15984..17063
                     /locus_tag="ACHALG_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFN2586955.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17060..17689
                     /locus_tag="ACHALG_001556"
     CDS             17060..17689
                     /locus_tag="ACHALG_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFN2586956.1"
                     /translation="MITVVIIDDDPFVTKSLQTILESTKEIRVLGIGHCAKDALVLYE
                     THRPDVLLTDIRMPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYL
                     IKQNLQAIIPSVKSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKE
                     VAAGKNNKEIADALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(17799..18026)
                     /locus_tag="ACHALG_001557"
     CDS             complement(17799..18026)
                     /locus_tag="ACHALG_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFN2586957.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18129..20717)
                     /locus_tag="ACHALG_001558"
     CDS             complement(18129..20717)
                     /locus_tag="ACHALG_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HFN2586958.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20737..21396)
                     /locus_tag="ACHALG_001559"
     CDS             complement(20737..21396)
                     /locus_tag="ACHALG_001559"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFN2586959.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21445..21948)
                     /gene="trmL"
                     /locus_tag="ACHALG_001560"
     CDS             complement(21445..21948)
                     /gene="trmL"
                     /locus_tag="ACHALG_001560"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFN2586960.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22169..23035)
                     /locus_tag="ACHALG_001561"
     CDS             complement(22169..23035)
                     /locus_tag="ACHALG_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFN2586961.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23144..23785
                     /locus_tag="ACHALG_001562"
     CDS             23144..23785
                     /locus_tag="ACHALG_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFN2586962.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23847..25208)
                     /gene="mgtE"
                     /locus_tag="ACHALG_001563"
     CDS             complement(23847..25208)
                     /gene="mgtE"
                     /locus_tag="ACHALG_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFN2586963.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25226..26125)
                     /locus_tag="ACHALG_001564"
     CDS             complement(25226..26125)
                     /locus_tag="ACHALG_001564"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFN2586964.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26127..26924)
                     /locus_tag="ACHALG_001565"
     CDS             complement(26127..26924)
                     /locus_tag="ACHALG_001565"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HFN2586965.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(26902..27606)
                     /locus_tag="ACHALG_001566"
     CDS             complement(26902..27606)
                     /locus_tag="ACHALG_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HFN2586966.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27737..28312
                     /locus_tag="ACHALG_001567"
     CDS             27737..28312
                     /locus_tag="ACHALG_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HFN2586967.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28430..29107
                     /locus_tag="ACHALG_001568"
     CDS             28430..29107
                     /locus_tag="ACHALG_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP adapter SpxH family protein"
                     /protein_id="HFN2586968.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         8563 a         6169 c         5232 g         9143 t
ORIGIN      
        1 atacagtatt atttcggttg cttgctgctt cacctagccc ttgtactcgt gtttgattgg
       61 cgacttgacc aaattgattc ccgacgtcta ataaaataat agctaaaagc aaaatggcca
      121 aatggcttcc gccaaccaat aaagcaataa agcttattgt ttgcataaca agtcccatat
      181 acacgatttt tctttcagaa taagaatctg ccagtcgtcc cacgataggt gcagcaactg
      241 ctccagacaa accaaaaatc gctaatatcc ctgcttcgaa tgttccccaa ttgtaggcgg
      301 gactgctgat ataaaaaatc aacgtagacc aaaagatcga aaatgttcca aacatgaaaa
      361 atccactgac tgccgcttct cttaatagac gttgagaagc aactaaatgc ggcaaacttt
      421 taagtgaatc aaggtatgaa acagctgtac tactttgctg gacagaacgc ggcattttcc
      481 agtaaagcag aagtgtaaga attccgactg cacataaagc aaataaatag atcatccgcc
      541 aagaaaaaag actggcaaca ataccagata ccgtccgaga aagcaaaatc cccacgagta
      601 aaccgctaag caatgtaccc atgaccttcc ctcttgcttt tggtcccgct agtaccgcac
      661 cgtaaggaat gatgatttgc ggcacgatcg aaagtaatcc tattaaaaaa gaagcgcatg
      721 caaataacgt aaacgaaggc gcaaaaaaag cggccaacag agagacggca gataacgcta
      781 ccattcgaat gatcaatttt ggacgatcca ccacgtcccc taaaggaact aaaaacagta
      841 accctaacgc atagcctaat tgggtcaaca tcgtcaatat cccgactgca cttgtgctaa
      901 cagataaact agctgctact tttgttccaa tcggctggat gtaatacata ttggcaacaa
      961 caacgccaca agtgattgat aatagtaaaa taagcgaagt tgttaaaggt gttttttcat
     1021 tactcttcat tgatttcccc catttcctat cttagtttac aacttatgga aaagattgga
     1081 aatagaaaaa tacaactatt cgccacactt attatttgtg cttttgtttt ccttgttttt
     1141 tcttgcgatc tttataaatg aagacaacta accctactag cacagtaaca cttgcaacca
     1201 acgtgactgc agttttgtta tatccgtgaa acagagcttc tccaccacac gcatacaaca
     1261 cagacgaagg gatgactcca atcacgatcg acaatatgat ttgctgtaga ggcaatttca
     1321 tcgcatctgc agcaaaattg accatagaag atggaataac cggtatcata tagcctaaca
     1381 ttacacctat ttttggatgt ttcatttgtc ggattgcttg cacccaatga ttcgtttcgg
     1441 ttttcttatc taaaaatttt aagcgatgca taaggaaaat tgcagtgatg ttaccaagag
     1501 cattacccaa aatgttgatg atcgcacctg tcagtggtcc aaagctcaaa ccagcaatga
     1561 ctccaactac tgaagtagga atacctggaa cagcacacat caccgctgtc aatacgatca
     1621 agagtatcga tgcaaaagtc ccatgcgaac gaatatgtgc gagtagctct gccttactcg
     1681 ctctcggatt taaaaataat tggatgtctg tctgatattc taaatacaac cgatagccca
     1741 ctaccagtat cagaatgatt cccaacgaga tcaacatcaa tcggattatt ttctttttca
     1801 tatttcctcc tgaaaaaaat ctatattcat taaataatag tttaacataa aaacaaaaaa
     1861 gcaccgaaat ccaacatagg atctcagtgc ttttaaatag taaaaattat ttaacagtta
     1921 cagatgcgcc aacttcttca agtttagctt tcaattcttc agcttcttct ttagatacgc
     1981 cttctttaac tggtgcagga gcgccatcaa ctacagcttt agcttctttc aagcctaaac
     2041 cagttgcttc acgaactgct ttgataactt taactttttg gtctccagct gcagttaatt
     2101 ctacagtgaa ttctgtttgt tcttcagcag cagcagcgcc accagcagca gctacagcta
     2161 caggagcagc agcagatacg ccaaattctt cttcaatagc tttaactagg tcgttaagtt
     2221 ctaggatagt tgcttctttt aactcagcaa cgatgttttc aatgttcaat gccattataa
     2281 tttcctccgt ttaattgttc ttttttattt aatgctttat gcggcaatta agctgcatct
     2341 ccatcttctt tgtctgccac tgctttgaca gcgtaagcca cgttgcggac tggcgcttgt
     2401 agtacagata gcaacataga tagaagtcct tcgcgatttg gcaattttgc caatgctgtg
     2461 atttcttcca atgaagagac tttaccttcg atgataccgc ctttgatttc taatgctttt
     2521 gcttctttag caaattcgtc catgattttc gctggtgcga ctacatcttc gttactgaat
     2581 gcaacagctg taggtccagt gaatacttcg tccatgcctt caaggccagc ttttttagct
     2641 gcacgtgaaa gaatagaatt tttgataact ttcatttcta cgttagcatc acgaagttgt
     2701 ttacgtaaac gtgttacttc atcaactgtt aatccgcggt agtcaacgac tactactgat
     2761 gcagctgctg taaatttttc gcttacttca tcaacgattg ctgctttttt tgcgataatt
     2821 gcttcactca tttttgagtt tcacctcctg aattttgatg gaagagtttt ttgcactgaa
     2881 aaggcaagtc acttttatag ttactttgct caaccagtat tattcatcac ctattcactt
     2941 cgttcatagt gattctactc gctttgctcg aaaggaaact cctttcgctt tgctcaacca
     3001 gtattgttca tcatctcttc acttcgttca tagtgattca caataaaaaa ctctatgcca
     3061 ccgcagacat agagggacta ttgaatatat tcaataaacg tcctcggtag gaaattaagg
     3121 cttgatgcca cctactgtct tcggtacaag ttaattatta acctcaacta gcttagcatg
     3181 ctaaactagt tgagtcaaga attatttttc agttggatca atcaacattc taatatttaa
     3241 ttagaaagaa gcttggtcaa catggatacc aggtccaaat gttgttgtga tcgttacatt
     3301 tttcatgtat tgacctttag ctgctgatgg tttagctttt agcaatacat catggattgc
     3361 tgcaaagttt tcaactaatt tttcatcgtc aaatgaaact tttccgattg gtacatggat
     3421 atttccagct ttgtccacac ggtaagttac tttaccagct ttcacttcgt ttactgcttt
     3481 tgttacgtcc attgtaactg taccagtttt agggtttggc attaagcctt ttggtcctaa
     3541 tactcgtcct agacgaccta cagtagccat catatcaggt gttgcaacaa ctacatcaaa
     3601 gtcaaaccat ccgttttgga ttttttgaac catgtcgtcg tcacctacga aatctgcacc
     3661 agctgcttca gcttcttttg ctttgtctcc tttagcaaaa actaaaacag tttgtgtttt
     3721 accagttccg tttggtaata caacagcacc acggatttgt tggtctgctt ttttagggtc
     3781 tacgttcaaa cggtatgcaa cttcaactgt tgcatcaaat tttgcaatgt ttgtttcttt
     3841 tgctaaagct actgcttcgg caacaggata aactttactt gaatcaactt tttttaatgc
     3901 atcttgcatt tttttgctct ttttagccat tttggttcct ccttgattgt ggttataacg
     3961 gttgtacctc ccacgtgttc tatatctttc aatatagagc caactgagaa gctgctgtct
     4021 tagggtgtat agattattct acagtgatcc ccatgcttcg tgcagttcct tcaaccatgc
     4081 gcattgctgc ttcaacgtta gctgcgttta ggtcttccat ttttaattca gcgatttctc
     4141 ttacttgatc gctagaaact ttcgcaactt tatttttgtt tggttcgcct gaaccttttt
     4201 cgattttagc tgcttttttc aatagaacag cagctggcgg tgtttttgta acgaatgtga
     4261 atgaacggtc ttcgtataca gaaataacaa ccggaatgat caaacctgct tgatcagctg
     4321 tacgagcgtt gaattctttt gtgaatccca tgatattgat acccgcttga cctagtgcag
     4381 gacctactgg tggagctgga gttgctttac ctgcaggaat ttgcaattta acgatttttt
     4441 ctactttctt tgccacgaga catacctcct taagtccgtg atgtggttaa ttggagatgc
     4501 atatttctcc tcccacgttt tatctgatct gaaaaaatcc agacaaataa tgtgcaaaac
     4561 aatgcacact ggtacagtat agcaaattta tttgtttttt caagattttt ttctgctatt
     4621 tttacatttt gcttgaataa acgctaaatt tattctaaaa aaacaacaat ttttcgttct
     4681 catttcttct tttatgttac acttgcgcta gcatggttca acagatgaaa gcaaacaata
     4741 acacggtttt ttagaccctt gtacaaagat tcctacataa caaaatgagg tggagaaaat
     4801 gaaagattta aggtatagaa gtgtctttga tattattgga cctgtgatga ttggtccaag
     4861 tagctctcac actgccggag ctgctagaat cgggaaaatc atacgtagta tatttggcga
     4921 acagcctgat tctgtagata tttatctata tgaatcattt gcaaaaacct atcgggggca
     4981 cggaacagat attgcattag ttggcggcct gttagggatg gaacccgatg atgaacgttt
     5041 agctgattct ttgaaattgg catatgaagc cggtatggaa gtcttgtttg ttcctaagag
     5101 tgaaaaagcc gaacatccaa actctgtaaa gatgcttcta aaaaaaggaa cacgaaagct
     5161 ttcagtcact ggcatttcga tcggcggtgg caatatccag atttctgaat tgaatggatt
     5221 caaaatttct ttaagcatgg gtactcctac cttgatcgtt gtccaccaag atgttccagg
     5281 tatgattgct aaagtgacta gtatactttc tgagaccaat acgaatatcg gcactatgac
     5341 agtgacacgg gaatcaaaag gtgaaaatgc cattatgata attgaagtag atgatcctca
     5401 agtagaagaa acggtaaaaa gactcaaaca gctgccaaat atcgacagcg taaattattt
     5461 cgattaagga gctaaaaaaa tgtttttatc gattgaagaa ttagtagatc aagcaaaagc
     5521 ttatccaagt gtagcagaat tgatgattgc agttgaaatg gaaatgagtg gccgttcccg
     5581 tagtcacatc atcgagatca tggaaaaaaa cctctcagta atggaacaat caatcaacga
     5641 gggaaaagaa ggtgttacct ccgtcacagg aattaccggt ggtgatgcaa aaaaattaga
     5701 tgattattta aatgaggcag actttttaag tggagaaaca atcttaaaag ctgtacgcaa
     5761 tgctgtagct gttaatgaag tcaatgcaaa aatggggttg atttgtgcta caccgactgc
     5821 cggaagcgcg ggtgttgttc ctggtgtgct gatggctgtc cgagatcggc ttgatttgac
     5881 acatgaacag caattggaat tcttatttac cgctggcgct ttcgggttag tcatcgcaaa
     5941 caacgcttct atcagtggtg cagaaggggg atgtcaagca gaagttggtt ctgcaagtgc
     6001 aatggcagca gcagctttgg tttgtgcaaa aaatgggact cctcatcaag cagcacaagc
     6061 agtagccatt acattgaaaa atatgatggg actgatttgc gatcccgttg caggcttagt
     6121 tgaagtcccg tgtgtcaaac gtaatgcttt aggctcttct caagctttta tctctgctga
     6181 tatggcatta gcgggtatcg aaagtgtcat tccgccggat gaagtcgttg ctgcaatgta
     6241 ccaagtcgga cgtcaaatgc cgtcgatttt caaagaaaca gcagaaggtg gcttagctgt
     6301 tacgccaact gctaaacgac ttgcggaaga gattttaaat aagagggcgt gataaagctc
     6361 cttaatttcg agtattaaga gattagttat cttcataaca aaaaacgaag agaggactaa
     6421 ttgcccttct cttcgttttt tattaatcca tcaatttcgt attttttact tcatcgatcg
     6481 tttttgttcc tgcaagttgc atcgtgatcg acaattcttt gttcaggtgt tcaaaaacag
     6541 atgttactcc ttctgcccca cctagattca acccataaat cactggtcgg ccaatcgcaa
     6601 ctaagtcagc accacttgcc agagctttga agacatgctc tcctcgacgg acaccagagt
     6661 cgaaaatcac tggtacacgt ttgttgacta cttcggcaat ttttggcaat acttcaaagg
     6721 acgcaggacc tccatctaat tgacgtccac catgattgga tacccagata ccatctgccc
     6781 ctgcagagat agcgattact gcatcttctg gcgattgtat tccttttacg ataactggaa
     6841 gatgggtgat ttctttgatt gttttgatgt catctggtgt caagccttgt tttgcagctg
     6901 cataaatttc tgcgattcct tttccttcgc cattaccact tgcactttgt tcgctatacg
     6961 cagctaagtt aggcattggt aatggaaatt ggaattgatt gatgacgtct tcttcacggt
     7021 aaccaccgag cgttgaatct gccgttaaaa tgatagcttt agcccctgct tctactgctt
     7081 tgttcaaaat gaactcattg aatctgtcgt ctttactcat atataattgg aaaaactgcg
     7141 gtgcgcctgg cgctgcatcg gctgcgtctt tgattgtcgt atttgcataa gtgctgatgg
     7201 aaaagattga tccagcagca gctactcctt tagctgtatc agcttcccct ttttcatgtg
     7261 ccaatccttg ggccgctgat ggcgcttgga taatcggtgt atctaattca attccgaaaa
     7321 cagatgtatg caaatcggct gagtcgattc cgcgtaatat ccgtggcatg attttctttg
     7381 tattaaaaga agtcgtattt tctttcatcg tccactcatc ttcagaacca ccgcgaatat
     7441 agccaaaagc tcctgcttcc atgcgctctt ttacacgacc ttctagttag gcaatgttta
     7501 caatatcgat tggatgttct tcattacttg tatgataagt catagtagat ccttctttca
     7561 tcttcttgat tacatataaa tcatactaaa cttacaaaaa gtaagcaaac atttactctc
     7621 agatactttt atcgctattc tttccaaata aatcgactta caagctgatc tacttcttgg
     7681 ttttcatgaa gcatactatg agaggcattt tcacctgtca ctactttttc ctctatttca
     7741 tttccgcgtt gcttcaacaa cgcatagacc gctaatgcac tgtttaacgg tactgttcca
     7801 tcactcaagt ctgtttcatc tagttgacca gcaatcaaga aaaatcgagt ggttatagga
     7861 atattaccga tcatttgctg gtaatcttga tagcggttag aaacgacagc aggccccttt
     7921 gctaactcat ctgtcagaga ctgcgcacta tcatcgacaa aatcattaaa aggcgcgcca
     7981 attgctgtta atgtctgaac ttgtggcaat gacgtatcct gtccatacgt tcctaaatag
     8041 cgtagtgcac taactcctcc catggaatgc cccactatgt tgactttatc tatgccgtag
     8101 ttttctttca gatacaaaag acaagttttg atccattctg cttgattcca ctcgttattt
     8161 ttattatctt caaacaatac ttggaccatc ggattgtctg cttttccaga caaatctcct
     8221 gttgcttgta tttgtccatc tgttcctact gttaaaacca attcttttac tgcttgctga
     8281 tttttttcca aacgccgcaa catcccttta aaggaatttt ctgtcccgct gtagccgtgg
     8341 aaaaaaagtg ttggaatagt ggttttttct atcactttac tggattctgc cgttttttct
     8401 ttatcagaaa cttcagtagt atttatcttc gtcgtctggc aaccacttaa cgcaaacaaa
     8461 ctgataagga ctaaagctgc tgcagttgca cgtttgcata aatttttgat tggttctgtc
     8521 atacacctct tcctttccat atagaaaaaa gaaagacttg aaaggccaat catatgatcg
     8581 acatttcaag tctctttatt aatcgatttt atctacttga tcgaaatcaa gttctgtact
     8641 ggtttcacgt ccaaacatat cgatgttgac tttcaatttt tctttttctc cgtcaacttc
     8701 tgttacttgt ccttctaatc cagcaaatgc gccttcgatg attttcacga cttcgccaac
     8761 ttctacttct agatcatttt gtcgcaggct cataccgata gaacgcaaga tatgattgac
     8821 ttcttctgga agtaatggtg ctggtttgct tccggctccg tgtgaaccaa cgaatcctgt
     8881 cacaccaggt gtgttgcgga caacatacca tgaagcgtct gtcatgatca tttcaacaag
     8941 tacgtatcct gggaatgttt tatgaacgat ttctttttct ttcccatttt tgacttcagt
     9001 ttctttttct tcaggaacaa cgacacggaa aataaagtcg gccattttca tactttgcgc
     9061 acgagattcg atatttgctt ttactttgtt ttcatatcct gaatatgtat gcaagacata
     9121 ccagtttttt tcaaaagatt ccattgaagg ctcctcattt cattgtaaaa taaaaaaacc
     9181 tcagtacacc cgaagttttt ctttcgcttc attatagcac taaaagtacg ttgtggctag
     9241 cgagttattt taaaatccag ccaaataagc tttggatggc tgtatccatt acgtaaaaca
     9301 tcaatccgaa aataatagaa gtttcgatga ctactaacgt atctttacgg agttgttttc
     9361 tgcttggcca agttacttgt ttgatttcat cttttacgct ttttagaaat ttcatgagac
     9421 aacctccctt gctactgttt atttcgtttc tttatgtaat gtatattgat tacaatattt
     9481 acaaaactta ttgatttcca aacgttcgcc gctttttcct tcactgactg attttgtgta
     9541 gttgcgagac ccacaaacgg aacaagcgag tgctgctttt ttctttgcca tttacttctg
     9601 cccccttgct gagaatcaga cttatacttc ataacagtat cacgtaactt tttacttgtc
     9661 aatttgtgaa ataaactatc tattgttttg gataaaatta tgatatgatt aatttgaaat
     9721 taagctaaaa tgagacaatt ggaggtatag cgatgctatt aaaaacactt gtatataaga
     9781 aaaaagatct gacaaccgtt acagagacta gtacattaga agaagcatta aaaattttag
     9841 aaacttccgg ataccgttgt gtgccgatct tagattcttc aggtaatatt ttccgtggaa
     9901 acatttacaa aatgcatatc tatcgtcata aagcaaatgg cggtgatatg acgcttcctg
     9961 tcactcatct tttgaaaaac gctacaaagt tcatttacct ggatacttct ttcttcaaag
    10021 tattctttac gatcaaagag cttccttata ttgctgtcct tgacagtgaa aatcattttt
    10081 atggtatttt gacacatagt acgttgctta atatgctctc tcaatcttgg agcgtagaac
    10141 aaggaagcta tgtactgaca attgcttcca caggaaagca aggagatttg gctgctattg
    10201 caaaaatcat ttcaaaacac agcagcattg ccagctgtat tactcttgat atcgaaaaag
    10261 acgaatacat tcgacgtaca ttgatcacgc taccggctaa cactgaagcg aaagtatgtg
    10321 aaacgatcgt caaccactta gagaaaaaga attataaagt ggtagaaatt gaaaatttgc
    10381 aaaaccaaga agcataatca aaaagcgaaa aaagaaaaca gctaatctgc tttctttttt
    10441 cgctttttat aattagacac gcttttcaaa atcaagcgcg cgatctaaat aatacatcaa
    10501 tggtgctgaa acagccatga cgataaactc acttaatgca agcgtgccat aggttgtcca
    10561 aaatgcttgg ccacctgttg gaactaacat ataagcaatc agtcccatgc tgatggtaaa
    10621 gaagatcgtg ttcaatacta aacgcatctt tacatttttc actttgtttt gcaataaagc
    10681 tgtcaggcct aaagaaagca atgtttggcc gccaccaaaa accacatcca tgataccaaa
    10741 accaaagaag gcattataga cgatgacccc gcctaaaact ccccaaagca atttacggtt
    10801 aaagacaact aaatgattca aactttctga aatacgaaac tggatcggcc ctgaggatac
    10861 aggcgcaact aaaatagtta acgctagata aagagccatg atgatgccat ttacaacgat
    10921 tacggataac cgttgtctgc ttttcgtttt ttgcattttc tctcctcttt tctcctagtt
    10981 tttttaacgc gggatggttg atgaaccgcg gttatgtgaa acaaacaata attataacag
    11041 acttttcatt tatttcaatc cgtcttatct gcttttaaaa aagcaaaagc ttaaacaatg
    11101 taaacgtttg ctaaaaatcg tgggatatgg tataaagtaa gtgtaagggg tcataagaat
    11161 tcccagtaaa gcagacgaag taggatctgc aagaacagaa ttgttgtgaa acttaaataa
    11221 ataaaaaagg atgtgggaat ttgagcaacc tcttactaga gaacggtcta gcaagcagct
    11281 gaagatctaa gtggacatgc cgaaccttgg aacttctcct tacgtaagta atggatcatg
    11341 cgcttgctag gcttttttgt gtttttcggt cttcggacct ttttcatttt cctagacttc
    11401 tgctataata accaagaatt aattttgtaa cagatttttt tgaaaaaaca agtttctttc
    11461 attcaaaaaa tagaaaaagc ttgctaatta ttggttaacc agcaaacagg agtattaaaa
    11521 tatcttgttt tattgaatag caggaggaaa tcatgaaatt ccattatcat atattgatca
    11581 atccatcggc aggcagcgga aatggctata aagtagcaga aagaatttta ccggtattaa
    11641 aaaataagca tattgactat acacttcatt atagtgaata caaaggacac gatgctgaga
    11701 ttgctgaaac tttggcaaaa gaaacattga ttccttggat agaagaagaa cagaaaacaa
    11761 tcgatacttt cccccttttg atcatcgtag gaggtgacgg caccttacat caggtacttg
    11821 atactttcta tcagatggaa gtagaattcc cagttgctta tattccagca ggttctggaa
    11881 atgattttgc tcgaggggca ggccttccaa aaaatcccaa aaaaggactt cagctgattt
    11941 tagcagctca atcaccagaa aaagtccaca taatggctta tgaagaaaaa ataagtgaaa
    12001 aaaaaggaat agctgttaat aatttcggta taggattaga tgcagctatc gtccatgcga
    12061 caaatcattc ttccacaaaa aagcgattga acaaatacaa cttaggatca ttttcgtatt
    12121 tattctctat tctccgtgct ttattcactc aaaaaggatt tccaattctg gtagatgcag
    12181 gaggaaaaca attaagtttt tcaaacgcat ttttatgtac agcaacgaag catccttttt
    12241 ttggcggagg aatcgcgatt gttccaactg ctgatgcgtc taaaccagtc attgattttg
    12301 tcgtggtcga acgaatcaat ttctttaaaa tcttgtggct cattctttta cttatacaga
    12361 aaaaacaatt gaaatcgaaa tatttccatc actacacaac aaacagatta cgtatcgttt
    12421 caacaacacc gcaacatggt caagaagatg gagaagaaat ggaaaaacga ccttttgata
    12481 tcacaataac cacaactaaa cagttgtttt ggtgttaata aaagaggttt tgtcagaagc
    12541 tacatctgga acaccgttta ttcggaaaaa aggactgtga cacgactttt gtcacagtcc
    12601 tttttttact atgcagttaa ttctttgccc aattgggttg ccatcgatag tttctttctt
    12661 tacccaaaat aaattggatc attgaaaaca ccgcagcaaa agcaactaag acacaaccat
    12721 tgaaccaaaa agattctgca aatgtttgat tgtaactgag tgtttcactg atcaagttat
    12781 tattttgaac aaaccaatat gttggtgtga atgccgctat tttgctaacc atttctggca
    12841 agtattctgc tggtacaaac accccaccga taaagcaact gcctgtgata aaaatatctt
    12901 tgatcccacc gctggtttct gagtttttta ccaatgaagt gatacaagcc gaaaagctaa
    12961 tgatcggcaa taaaaacaag aaagtattta aaatatgata ccataccgtt atatcccacg
    13021 aggtgtggat cactaacaga accattagaa gtaagaagcc actgaacaac agcagcgagt
    13081 aactaatcaa actaaaggtt actttgcgat tcaagtttcg tttggtaatg ggcgcactct
    13141 ggtttcgttt actgatctct ttgcgattaa acgctagatt gatgacgctg aaaccgctaa
    13201 aaatggatag aaacaaccca taagacaaga aattaaaggt tgtgcccgtc aacagctttt
    13261 tcatccgctg actatatccc gcacttagct caacttctcc agtaaccgag agtgccattt
    13321 gtgtctgttc catagctgca tcgattgttt tatcttcttc ttgaaacaga atcagtgttt
    13381 gcaaaaaggt gttcacttgc tgtgtaacaa ctgttttaga aaaggcatct gggcgactct
    13441 gtatcttgat cgtcgccatc tttcctactt cgacttgttc ggtaaagtct gcaggcagat
    13501 gtaagataat gtttacacga ttaaaataaa gagcatcatc gatttctttt tgtgaggttc
    13561 catccaatgt aacgactgtt tccttgtcat tcaaatattt acgaaggcca tcgactacag
    13621 gcgactcttc ttcactaatc aaggcgattt tcgcatcttg cagagaggta ttttcaacat
    13681 caatattttg cgcaaaaaag aatgtggcaa caacagtgat aattacacct atcagtaaat
    13741 tcgatttatt tgctttaagc aatgtccaaa tcgttttata aagcgtcata acgcacccct
    13801 ctttccatcc aagcatttcc taacgcaaaa aggaaaataa atccgatcaa ccatagaaga
    13861 ttggtataaa aactagccaa atcagtgtag aaataaagtt tatacaaact ttcgctgatc
    13921 aagttgacga gatttaattg accaacaatc ggaatatgaa gatcaatcca atacttcact
    13981 tgttccgacc ccatcatacc agcaaaaaag ctcatcgcaa tcgtcactga aatcaagatg
    14041 ctatcctttt gcaccagatt catttttggt atcaaattac caaccaatgt acctaacaaa
    14101 atcgcacaca acgaccccag agcggtaaca atcatcaacc attgccaacg ttcaccaaag
    14161 tctacttgat agataaaacg aaagacagct aacataatat aaatctctgt caaaacaatc
    14221 gtaaagcttg ctgccaaatt agcgattgaa actaacgatt gctttctagg aatcaaggac
    14281 aagcgaatac catttgacga ttgatttgct tgctgatcct gtacattgcg gaccccccac
    14341 atcgtcccgt agagaatcgc cattccaatc agagtgaaga aataaaaact tttgaaactc
    14401 atattacgac ctgcatgaac ttcttgaaaa gatacttctt gttcaaaaac agtcatgatg
    14461 ggcgcgagat caacgttctc cacttctgcc actttactcg aaattatacc aatacgctgt
    14521 aaaaattgat ccatgacgtt tttcatcaac gtctgactca ttcctgcttg acccacatgg
    14581 agcgaaacag atgaaccatc aatgagataa aaaccagcca aatcatctgc atttagcttt
    14641 tcattcgctt ctacttccga tagttcctgt acttcaaata atggtttatc attgacttcg
    14701 acttgttcta agactgtggt aaaattttcc gaaagcgctg tttgatccgc tttgattaca
    14761 attccagtat taatcgtttc aagattgcca ccttgatcaa tctcgccaaa ggccgccata
    14821 aacatcaatc ctaaaagaat tggaaaggcc aatgtccaaa acaacatgga tttatttcgc
    14881 agcagtattt ttgtgcgata tttataaaaa tgccaaaaca ttatttcccc tcctctagtc
    14941 tctcaaatcc ttgccagtaa tctctaaaaa gacatcattc aaggacgctt ctttggttgc
    15001 tatttttccg taactgatct tttcttcttg taagattgta agaatcggca acaatactgt
    15061 atttagttca gtagttgtta cttggagctg acctttttta taagcgattc catcagaagc
    15121 tattgttcgc tgaatcgctg acaacacatt ttctgggagt aatgggatgt cgatcgtcaa
    15181 acgatccgcc gtccgtacca tatcttttag agattctttg gttcctttag caatcacctg
    15241 tccttgatcc atgatcacga tctggtcgca aagaatttct acttcttcca tgtaatgtgt
    15301 tgtatagaca atggttgctc cttgtcgatt cagctccttg atactttcta aaattttatt
    15361 ccgactttgg gggtctacag ctactgtcgg ctcatctaaa aaaattaacc gcggcttgtg
    15421 ggcaatccca catgcgatgt tcaaccgacg tttcaagcca ccactaagtt ctcctggacg
    15481 aaacgaaaca tatttttcta atcccactaa ttgaatcacc tcttcaatca aggctttcct
    15541 tttctctttt tcggaaacat ataaaccgca aaaataatcg atattttctt gcacacttag
    15601 ttcgtcaaag accgcaacgt cttgaggaac gaccccaatc attgccttga cagcataatt
    15661 ttcaggagtc attgttgtcc cgaacaattt aatggttcct ttgtcataat ttaacaaaga
    15721 aagcatacag ttgatggcag tcgatttccc actgccgttt ggacctaaca gacctaagac
    15781 ttctccttct tgtacttgca gatcaaaatg attcaatgct acaaaatctc catagcgttt
    15841 gactaattta tcaatttcga taatcatcat gaaaaactcc ttccaaattt cttgttgatc
    15901 tgactataat aaagtgcaag aaaagaaact agtgacgatt ggctaaacca cacatgacat
    15961 ttgtcacaat gaaggagtct gctatgcata aatctcggct actcaacgaa actagctttc
    16021 tactggttat ctcattgctt ttgctggtaa gtcaaggatt cgttgctatg caagttatct
    16081 atttattgat cgccgtttta ttcactggtg tcagttattt actgcctaca aagccaaatt
    16141 ggattttcca agttattcta tttcttttat gtgccttttt acctgatctt gtcctgtttc
    16201 tgccagcgac gatgcgagtg atcgcccctg aagaaagcca ccttgattac ttgtttttgt
    16261 taggtttttt gttgatttcg acggttggta atcatccccc catcaaggtc gcctttgtcg
    16321 taattttgct gtttttttcc ttttacttac gaaaaaaaga tcaacgaacg accgatcttg
    16381 agaaaaagta cattgtctcc aaagatactc attgggaaaa acaacaagag ttatcggaac
    16441 aaaataaagc attggccctg tcacaagaaa cacaattagc cttgcaaatt gtggaagaac
    16501 ggaaccgaat tgcccgagac atccatgaca atgtcggtca tctgctgtca agtgcgattt
    16561 tacaaattgg tgcgcttgaa atgatcaatc aagaaccaaa actaaaagaa ccattagaaa
    16621 atttaagtgc caccgttcac actgggatgg atcggattcg tcaaagtgtc catgacctcc
    16681 atgagacatc gatctctttt caacactcct tagagtttct gatcgctgat tttccttctt
    16741 ctgttacgat tgaaggaaac ttgtttgaag gattgaatga aacgcagcaa aactgcttct
    16801 taatggtcat caaagaagcc ttaaccaaca gtatcaaaca tagtcatgcc aaaaccgttg
    16861 ttttacattt tcgaacgttg ccggcctttt atcgcttgca aatcacgaat gatggcacca
    16921 gcccacaaaa aaattctgct cccggtattg gtttgaaaac gatgcgtcaa cgtgttcaag
    16981 aacaaaaggg acaagtccat attttccaag aaaaccagca atttcagtta acgatcattt
    17041 taccaaggga gggacaccaa tgataacagt agtcattatt gatgatgatc catttgtgac
    17101 gaaatcgtta caaaccattt tagaaagcac gaaagagatc cgtgtgttag gcattggtca
    17161 ttgtgccaag gatgcgctag tcttatacga aacccatcgt cccgatgttt tgctgacgga
    17221 catccgcatg cctaaacaaa cgggcatcga tgctgccaaa gaaattttgg ctaaatttcc
    17281 caaagccatc cttttgcttt tgacgacatt caaagatgaa gaatacatcc gcgaagcatt
    17341 ttccatcggt gtcaaagggt atttaatcaa acagaacttg caagcgatta ttccttctgt
    17401 caaatccgct tataatggac aggccgtatt tggtaatgaa atcatcgaga ctttcactca
    17461 aatgatgaag aataaaccga cgattgatag tcatgcttat gcttctttta gtgaacgtga
    17521 gcttgcaatc atcaaagaag tagctgccgg gaaaaacaat aaagaaattg cagatgcctt
    17581 gtacttgagt gacggcactg ttcgcaatta catcagccaa ttactagaaa agctagattt
    17641 acgggatcgc acgcaattgg ctatctattt ttatcagcat ttgcaataag tcttatctaa
    17701 cacaaaaaaa cctgaatgat gctccttcgc atcattcagg tttttttcac atcttctatt
    17761 caattgtccc gcaaaccatt tcctcacgat tgaaagaatc aaggattcgc agggctgcca
    17821 ggattcgttg gattgttcgg cgtgttaggc ggaaaaatag gcgccttgtc taacgttttt
    17881 tctagttttg attcttcacg atcgtctcgt tctacatcag gactttcatt caatttgaat
    17941 tctttttcca tctcatctaa ctttttaaag tcattgccag attcttttgc taatttcgct
    18001 aatttatctt cggtttcttt agccataaag taacgccccc cttttgattt cagcttagca
    18061 tatcacactg aaaaaacaaa agaaaacgct aaccaacgat catttttagc tatttctcat
    18121 ctttaaaatc aagcattttt tgccggtata aaatcgtagg gagaactgtt ttccatacca
    18181 atcatcggat ctactaactg gttatcgatc acttctgctg taagaacgaa atctttagaa
    18241 acagcaggtt ctacctcttc tttcttttct tctccaacaa atgaagaaaa cagatctggt
    18301 tccactgttt ccattacttg gtttttcgtt tcattcgtcc cactgtcatc ttctacaggt
    18361 agcttttctt ttacaacccg tggctctttt gtatagttcc catcttcaaa tggatcattt
    18421 ccgaccaaag aatcttcata agctgctagt ttggtccgga gagtgatatt cattttatca
    18481 ttatccatga tccttgattt caacgtagtc atccggcttg ctgattcatt cgctacacaa
    18541 gtgacatagg cttgctcttc ccctaacaaa atcaacagtt ccttactgcg agtcactgcc
    18601 gtgtagagca aattacgctg aagcatccgc tgatattgat ggaccatggg caaaataacc
    18661 atcttgaatt cactgccttg tgatttatgg attgagcaac aataagaaag agtgatttta
    18721 ttccattcgt ttcttttata agtgacctca tttgcatcaa actggataac taattcatcc
    18781 actttatctt cagaatcttt tgccaagatg atacccgtga tttctcccat atctccattg
    18841 aatacattca attcgggagt attgactaat tggaggactt tgtctcctat ccgataaacg
    18901 gtgtcgttcc aatgtacttc cttttttcca ggtttccctg gattgaatat ctcctgcatc
    18961 attttattta acgcatcgat ccctgcaggt ccccgataca ttggtgctaa tacttggata
    19021 tcttgtgcac ggaatccctt atttttcgct ttttccacaa ttttagagat caagggttca
    19081 atttgataag catcacttcg gaaataggaa cgatcttttt gattctttgt gaaatctttt
    19141 ggcagacgtc cttctttgat ctcatgggca agaggaataa tactagaacc atcaccttgc
    19201 cgataaatct gattgagttc cattttggga atttgttcga tttccaaaag atcatggagt
    19261 acttgaccag gaccgacaga gggaagctga tctttgtctc ctactaagat aacttgcata
    19321 ttcgtcggaa tcgctttgaa taatgtgtta gccagccatg tatcgaccat agacatctca
    19381 tcaacaatca aaagtccgcc ttctaattct ttcgtactga ctgaaggtgt tttttcccgt
    19441 ccattcagcc ccagaagacg atggatcgta ctggcaggca gacctgtcgt ttcgttcatt
    19501 cgtttagctg ctcggccagt tggtgctgca agtagaatag gaaaaatttc ttgggtatat
    19561 tttgaaggat ccaattccag tccgttcaac tctgcaaaaa gattgacgat tccgttgatc
    19621 acagtagttt tccccgtacc tggtcctcct gttaaaataa atagagggga acggattgct
    19681 tcagcgatag cttgttcttg cgaaggtcca tactgtatgc cgaatatttt ttcgatacgc
    19741 cgaatattct tctgcacttc tttttcagga taatggattt cttttttacg actgagaagg
    19801 cgctggatcg aattaccgat cccccattca gaaaagtaca aactgttttc gtaaagatta
    19861 gtctcttctt gttgtatctt tccttcttca acaagttgga taattacagt agcaacctga
    19921 tcaggctgga tttcaattgg gcgacttgtt tctaaaatac ggatcgtttg ctgaagcaat
    19981 gtctccgccg gtacgtatgt atctcctgtt tgaactgcat gctgaaaaat ctgatgcaag
    20041 atagctgctc gaattcttct cggtgaatct gctgcaatac cgatttgttc ggctagatta
    20101 tctgcacgtt tgaacccgat tccttctatg tcttcgacta gctggtacgg attttcttcg
    20161 atgatatcca aagactcgtt cttataggcc tggtagatac tgaaagctaa ctgactaccg
    20221 aacccgtaac gattcagtcc catgatcact tgatccatcc cgtggtttag ccgaatcgtc
    20281 tcagcaagca tttctctttt tggcttcgtc aaaccggaga tttgttttaa taccgaagga
    20341 tcagcaatga ttttttcgat agcatcttct cccagcaatt ctacaatttt ttctgctgtt
    20401 ttcttcccga ttcctgggaa tttttcgctg gaaagatacg ctacgatccc atttgcagtt
    20461 gttggctttt cttgctcata tctttcgagt ttaagctgcc ttccgtattt aggatgatcc
    20521 actaattctc cataaaaacg ataagtctca ttttcttgaa tatcgccgaa acttccggtg
    20581 actacaatct ctttttcacg gtaatcagct gtggtttcac tgatgtgtac caaaagcact
    20641 ttgtaaaaat tactaggatt ttgaaaaaaa atcgcggcaa ctgtaccaac gaaataaggc
    20701 atttcttctg gctgcatcgg aagccgcctc cttcttctat agtaaaggtt ctggtgctgt
    20761 ttcagttcct aaaaaatctg tttggttcca tactttcagt tcatatggca ataatctgca
    20821 aggctcttca gtctctaaaa tcgtcaaact actattgaac aatccaccca tttcacgcaa
    20881 ctcacttagc tcttttcctg tcatccattg aatggctgca gttaaagaag ctccatgtcc
    20941 tacgaataaa agtgggccat ctttatgtcg ggcaaccgca tctgctacaa cagtttcgat
    21001 acgtgtgatt gccgattcaa tcgtttctcc gtgaaaagga gaaggatcat acagatctaa
    21061 atgattacgc aaattcggca gattatccgg atatttctgg tacatttcat cgatcgactg
    21121 accttctaaa gtacccagcc ctagttctct tagcttatct gtataaaaaa tctctaccgg
    21181 acgagcaagc tctttgttga taccttctgc tgtatcacgt gctcgctttg ctgtactgga
    21241 ataaattgct ttaaaaggaa catattttac ctttttccca aatgctttga tttcttcgta
    21301 actttgtggc aaaagcggag aatcgccatt gctcccttga aaacgtcttt cctgattcca
    21361 ttgtgtcttc ccatgtcggg taaaatatag ttccatacgc agcaaactcc tgtctttctt
    21421 ttattttact ctataataaa ctgtctaatc cagcttgtca ttttcatagt gatgcgtcaa
    21481 ttctaaatta gggaatcctt gctggcgaag tgcttcatac actatcattg cagctgtatt
    21541 cgataaattc aatgagcgga catgctcatc attcataggg atgcgtaaac acttttcttc
    21601 gttttcacgc ataaatgttt ctggcaaacc ggttgtttct tttccaaata aaaagaagtg
    21661 ctcttctcca tcagcaaaat tttcgtcact atatacttta tgggcaaatt ttgagatcaa
    21721 atgtagttta cgattaccta aaaatgttaa aaaagcttct aaattactgt ggtaagtaat
    21781 atttacatca ttccaataat ccaaccctgc ccgttttaga tgtttgtcat cagttgaaaa
    21841 acctagtggc tcgattaaat gaagcggagt attcgtggct gcacacgtac gggcaatatt
    21901 tccagtgttt gcagggatta atggttcaaa taaaacgata tgattcatca ggatcattcc
    21961 tctcatattt atgttcgttt ctattttctg acaatttgat ttttgcataa aaaaaaacgt
    22021 atcaactcgg tgtcagtcac tgcgagtcgt tacgttagtg aagctcttct cactaacttt
    22081 tatcagaaca ggaacctgat aaaaaccaat gaaaaaacaa cttgttgcta atataacacc
    22141 ctcaactttt ttttgcaacc tctactcatt aaaaactgct agaaaaacgt tttctcttag
    22201 ttccttttaa taattctatg tcaattgtaa tcttcttcaa aggattctta tataagtctg
    22261 ctttgattcg ctcttctacc tgccattcta aaggtgacat ggccaaaaga tctttttgat
    22321 tttcctctgg tatctcaaat tcataggtta cacgctgacg ttcagttaat ttcattgctt
    22381 cagcaaactt ggctactaca cgttcattgg aataactctg tttttcaggt tgattcggat
    22441 aaaaagcctg acgtagttcc tttaaataat tttcttgtgg caccaccttc aacacttctc
    22501 catcttctga gagtacacgt tggaattctt ggtaatgaga aggcgaaaag atatttaaaa
    22561 ccgcgttgaa tgtttgatca gcaaacggca aattggtcaa atcagctaca caccaaaatg
    22621 cttcggcaga ttgttctgta gctgcgtaga ctccttcttt tgaaatatca aatccaacag
    22681 cattttgaat ctttttcaat tttgaaagtt cattcaagaa gctaccttct ccgcagccaa
    22741 catctaatag cgagacttct tcaggtaaat gctgcgcaat agtctcaata acgggctgat
    22801 acatgccaga ttggatcatt ctacgccttg gaacgaacat tccttgatcg tattctgttt
    22861 taaccgcatg ggataaaaaa taaagtgttc cttttttgga aaggtcaaaa cggtgattat
    22921 ttttgcatat caagccatta tcagactttt ttacttcttc ttggcaaacc ggacagcgga
    22981 acagctgatg attttctgcc aaaaactggc gtcctcggtc aatttttttt aacatagttc
    23041 cccctcattt ctgttttatc ttaccgttag atgctcactt tggcaactgc taaaaatatg
    23101 atacaatgcc tagaagaaag tgaaagataa aggagaagat tagatgatca aagaattttg
    23161 cgctgaaaac tacacatcta ttcctctagc aattgctaga ggagccgatc ggatcgaatt
    23221 gtgtgacaac ttagcggtcg gtggcacaac cccgagtact ggtgtgatcg aagaaacgtt
    23281 aagttatgct ggggaaaaag atatccccgt aatgacgatt attcgtccta gagggggcga
    23341 cttcgtctat aacgatatcg aattgaaaat catgcatact gatttgattg aagcaaagaa
    23401 attagggacg gatggcgtag tatttggttg tcttactcct tcaggctggc tagacgaaga
    23461 ggccttagaa ctattgatcg aaacagcaga agggttgcag atcaccttcc atatggcttt
    23521 tgacagtatt cctgaagatc ggcaattcga agcaatcgat tggctggtag aacatggtgt
    23581 tgaccgtatc ttgactcatg gcggtcctgc tgacaaaact attgtagaaa atttcccacg
    23641 gcttaaagaa ttgattgcct atacagatgg ccgcttaatc attttgcctg gtggtggtgt
    23701 cactgcagag aatgcagcac atgtagctaa agaattgaac gtaagcgaag tacacggaac
    23761 caaaatcgtg gatcttcagt tataatctta taaacaaaaa aacatttgca acaggcataa
    23821 tgcttattgc aaatgttttt gtttttttac ataaaatagc ccataaataa agaagcaata
    23881 ttgaaataaa tcagtacact cgtcaaatcg ctcaaagtcg tgataaaggg accgcttgct
    23941 actgctggat caaatcctag acgatccatt agcatcggga ttaaactacc tgcaagattc
    24001 gccactgtga ttgcaaatag catagccatc cctatgacaa accccaagat aaaattatgt
    24061 ttccaaaaac ctacgattag aaaaatcgtg atccccgtaa cagctcctgt gacaacgccg
    24121 gtcaagactt cagccaaaac aagtcgaaca aatccatgat ctttctcatc agaaatcgca
    24181 agtcgacgaa cagccacagc tagcgactgg gttccagcat taccggcagt tcctgtaatc
    24241 agcgaaatga agactgctaa aatactcgct tcacttacca agtcttcata gtgactgatt
    24301 aaagaagcgg tcgacatacc taaaaacaac agagtgatca gccaaggcaa acgtcgagac
    24361 gctgccttta ctggattttc attaatttct tccacattga ccccggcaag tcctgaataa
    24421 tcgctagcag cttcatcatc aataacgtcg atgatatcat cgactgtgac gattcctaac
    24481 aaatgatcat cataatcggt aacaggaaga gctaagaagt cataatctct aaatgtttgt
    24541 gccacatctt cttggtcgtc gcctacatga actgaaagca ctcgttcgct catcagatca
    24601 gaaatcattg cgtcgtcatc attcacgatt aggtcgcgca gagaaataac ccctactagc
    24661 tgaccttctt gattaacgac atagatgtaa tagattgttt cagctacatc tgcttcgttc
    24721 ttcagcacgt acatcgcaga gcgaacagtt tgattggcta cgatcgaaac aaattcagtt
    24781 gtcatgatcg cgccagccgt ttcatcctcg taatggagaa gttccttgat ttcactggca
    24841 tcgtctgccg acatcaaact taaatattta gcaatttgct ttttatccag cgtattaagc
    24901 aagtcaacag cattatctgt atacatctca gaaagcatat cagctgcata gctaggacgc
    24961 atttcatcta ggtattcttt catatgttcg ttgtcttctt caatcacatc gaacatatcc
    25021 gctaattctt taggcgacag ataggaatag atcagctgcc tgttctcttc tgtaagagat
    25081 tggtagaatt gcccctgctc atagatatgc atctctaaaa aactgtttct gaatgcctga
    25141 atattatttt cccttaaatc ttgagtcaac tgggcaaatc tttcctctaa ttcttgattt
    25201 tcatccaaac caatctcacc tcgccttatt cttcaaaccg tctcacaact gcctccatgt
    25261 ctgcggctaa aggctgcttg aactccagca actcttctgt aaatggatgg taaaaacgca
    25321 gatcataaca atgaagggct tgtcttttta ttcccagatc cattcgcccg ccatacatat
    25381 catcaccgag cagggcacag ccaattgcgg aaaaatgaac tcttatttga tgtgtccttc
    25441 ctgtatgcag ctggatatca actaacgcga tttctgttag tcgcttctct atccagtact
    25501 cagtctgtgc cgaacgtcct tcatcagaga ccattcgctt caatagcgaa gaataatctc
    25561 gggcgatcgg caaatcgata tgtccatgtg ccgataaatc tgaaagcctt ccactgacta
    25621 aggcctgata ttttttcaca aacttctttt cacgaagttg catatctaat tttgcgtgcg
    25681 caaatccatg ctttgcaaaa agcatcaagc cagatgtatc ccgatctagt cgcgtcacca
    25741 catggattac ttggttttcg taaccttgtt ttttataata agcctttaca cgattagcca
    25801 ttgtcccatt tggatggtat tgggcaggaa tagacgaaac gcctgctggt ttatttacta
    25861 ccagaacgtg ttcatcttca tacacgatat cgatcggtgt ttcatccaaa agtacggttt
    25921 catgttctcc ttcagcagga atcacaatgg ttaccttatc atccttagct agagaaaaca
    25981 agacattttc tacttgatca ttaactttga tttgaccgcc ttgaaatttt attttcgcta
    26041 acagaccctt tgagatccct ttttctttta aaaaatattt tacttgctga gaatgatctt
    26101 tctcataatc ccatgtaaac tgcatatcaa tcctcactaa taaaggcatc tttcacacga
    26161 tgccaaaaat gcatatgtcg ataagaagca aaatggatcc gttcttctgc tattcgataa
    26221 taaattgagc ggatatttgc ttttgcgaca ttcaattgat caaccgttac aagataatct
    26281 tctgtatttt ccaattttat ttctagccat tcatcatggg caatcacgat cggagaaccc
    26341 aaagtacgaa acactcgatt attcagtgaa gcaatctctg ccaactgaaa ggcattgata
    26401 ctaggatgga tcacagcacc gccgacactt ttattatatg ctgtcgaacc agtaggcgta
    26461 gaaatcgtca atccatctcc tctaaaactt tcaaacagct cgtttttgat gtatacatcg
    26521 gctaccatcg tacgattggc ccgtttaatc gttgattcat tcagtgctaa aaaatgctgg
    26581 tccggtgttt catccaaata actgatccga acatccagca agggataact aacactttgt
    26641 tcccggttcg ttcgtaagct gtctactagt tcttccagct cgtaatcccg ccaatccgtg
    26701 taaaacccta aatgacctgt atgcacgccc aagaaacgca catcattcag acaatgactg
    26761 tagtgatgga acgccgacaa taatgtgccg tctccgccga ctgagatgac caattctggc
    26821 tcatgatcat ctcgttgaat ccctgcttgt tctaaaagtg cgttcaaacg ttctgtcact
    26881 tctatagtct ttgcttcttt attatgaacg atcgctactt tcatcccctg cctcctctct
    26941 ttcgctttct ttcttcaaaa gcgttcgata gtagacatca tctgattttc ctcttccatg
    27001 ggagaataac cgctgtgctt cttggatttc ttcacggata ctggacatct cttcatccaa
    27061 ctgataggca gcttctgccg cacgctgaag tcgttcactc atttcttctg gaaaaacgcc
    27121 ttgatattta taattcagcg aatgctcaat cgttgcccag aaattcatcg caagagtccg
    27181 aatctggatt tccgccaaaa ttttcttctc accgctgatc agctgtacag gatactcgat
    27241 caccagatga taagaacggt agccacttgc ttttttattt gtgatgtaat ctcgttcttg
    27301 gagaactttc atgtcttttc ttttacggat gatctcaaca acttgatgga tatcttctac
    27361 aaactgacac atgatcctta acccggcaat atcagacatt tcttcttcta aacgtcccat
    27421 tggaatatgc cgtaattgtg attttgttac aatactatcg actggcttga ctctcccagt
    27481 gacaaattca atcgggacat gtcgattttg ttcacggaac tgtttgcgga tcccccgcag
    27541 tttcactttc aattctgata cagtttgttc atatggtgct aaaaatgctt cccaatctcg
    27601 ttccatagct gatacctcta cttcattaac attcattgtt tattctacca tatatgcaaa
    27661 gttttttcgt actcttgatt tatgcattga ccaaaaaaag aaaattaggc aaaatagaca
    27721 aaaaggagtc gatcaaatga cacaaatgct tgaaatcgaa tataaatcta tgctgacaaa
    27781 gcaagagtac gagatattca tcgctcacta tcaattaaca gataaagatt tccgtgtaca
    27841 gactaatatt tatttcgata ccgttgatga acagctgaag aaacaaaatt gtggcctacg
    27901 gatccgtttc gttggtcatc aagctgaata tacattaaaa acacctgctg aaaaagggcg
    27961 tctcgaaaca acggatacct ttataacaga agaagcagaa gcatttatct tcgaaaaacg
    28021 tctgcctcgt tcaggcgcag tttttcaaaa actttctgat ttgaatatcg atcctgcttc
    28081 attaatccaa accggaaagc ttacaacaaa acgagctgaa ttccctattg aagaaggact
    28141 tttagctatt gatgaaagct ggggagacaa tctacacgat tttgaactgg aactagaggt
    28201 aggggatgca tctgtgggaa aaattgcttt tataaatttt ttaaaacgtt tttctttacc
    28261 ttatcgtcct gctaagaaca agatccaacg tatgcttgaa gctaaaaatt agaaaccttt
    28321 cattttcaca aggcacttga gttcattttt ttactttttt ctgttaagtt ataggatgtg
    28381 aaagaatcta gtcactagtt gtatagaatc tattttgggg gagattcata tgatcgaaat
    28441 ctatttattt gttaatcctt taggggaata ctgtttcgaa atggaacaac agttattaca
    28501 atttatcgaa gaagagtacg ggacaccatc aaaagaaaag atgcaatttc gctttcttcc
    28561 tttggtcaat ttgcagacaa ttggggatgt catgcaacaa aaaggaattt ctcagaacaa
    28621 tcttgaggaa cgtaatcact tattctcaat gacttattct gcagcattgg actgcaaagc
    28681 ggcgcagttt caaggaaaga aaaaaggccg ccgttttttg atcaaattgc aagaagcagt
    28741 cggctgtaat gacataccat attctcaaga acttgctgaa tccatatttg aagaaatcgg
    28801 cggcgatttg aatatgttta aagaagatcg tcagtctgac ttcgttaaag aagtattctt
    28861 ttctgatcaa aaaattgctc gggaaatggg catcacaaaa cacccttctg cagtcgttta
    28921 caattatgcc tgcgaccgtg attttggtgt tttagtagaa gtaaaaggta caagtagcct
    28981 ttggagaatc acagagctgt gtaaaacaac agataactca tatcaagttt ttaatggtga
    29041 acatttacat acaaatgatt ctcagttcca ttcaagaact agccatttac acttgctttc
    29101 taattaa
//
LOCUS       DAZABD010000034        28677 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000034 DAZABD010000000
VERSION     DAZABD010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28677
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.034"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            770..1939
                     /locus_tag="ACHALG_001569"
     CDS             770..1939
                     /locus_tag="ACHALG_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFN2586969.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
     gene            2412..2966
                     /locus_tag="ACHALG_001570"
     CDS             2412..2966
                     /locus_tag="ACHALG_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN2586970.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(3139..3576)
                     /locus_tag="ACHALG_001571"
     CDS             complement(3139..3576)
                     /locus_tag="ACHALG_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN2586971.1"
                     /translation="MFSSEENKPLYLDYVNHNLKIIIKENNLKHITLHGFRHTHCSLL
                     FESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFMSSTEEKQGSLQK
                     VKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            3624..4571
                     /locus_tag="ACHALG_001572"
                     /pseudo
     CDS             3624..4571
                     /locus_tag="ACHALG_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634498.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
     gene            4842..5277
                     /locus_tag="ACHALG_001573"
                     /pseudo
     CDS             4842..5277
                     /locus_tag="ACHALG_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345965.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
     gene            5293..6126
                     /locus_tag="ACHALG_001574"
     CDS             5293..6126
                     /locus_tag="ACHALG_001574"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN2586972.1"
                     /translation="MLYFSAIDNADFVFSDPFTAEVLSGIEEQAKNHGYFVLVHNVNS
                     AEDVKTIQRNWRFEGFIVVGVRVKEFLELNNVFDGPVVYIDTHLSKETLRELEQQMNR
                     LFVNTNDYQASTLAVEHLIHYGHEKIAFLSFDYIVGEPSVIQERHQAYIDTLKKYHLT
                     FKSHWIYNNSEFERIYQELAEFTAIVVTGDYLAAKLVKFLKNKNKRVMEQLSIIGFDD
                     ITFAELMDPALTTIRLNPGNKGAIAFHQLIGLAKQEEIKNQVLLLEGELISRDSVHKI
                     K"
     gene            6371..7396
                     /locus_tag="ACHALG_001575"
     CDS             6371..7396
                     /locus_tag="ACHALG_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN2586973.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            7534..7659
                     /locus_tag="ACHALG_001576"
     CDS             7534..7659
                     /locus_tag="ACHALG_001576"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2586974.1"
                     /translation="MKHFKKIALTGIAVLTGTLLAACGGGDSNSKDGQVTLSYAS"
     gene            7684..8799
                     /locus_tag="ACHALG_001577"
                     /pseudo
     CDS             7684..8799
                     /locus_tag="ACHALG_001577"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
     gene            8882..9778
                     /locus_tag="ACHALG_001578"
                     /pseudo
     CDS             8882..9778
                     /locus_tag="ACHALG_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338086.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
     gene            9794..10618
                     /locus_tag="ACHALG_001579"
     CDS             9794..10618
                     /locus_tag="ACHALG_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN2586975.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            10650..11960
                     /locus_tag="ACHALG_001580"
     CDS             10650..11960
                     /locus_tag="ACHALG_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HFN2586976.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            12108..13367
                     /locus_tag="ACHALG_001581"
     CDS             12108..13367
                     /locus_tag="ACHALG_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFN2586977.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            13377..14249
                     /locus_tag="ACHALG_001582"
     CDS             13377..14249
                     /locus_tag="ACHALG_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN2586978.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            14261..15091
                     /locus_tag="ACHALG_001583"
     CDS             14261..15091
                     /locus_tag="ACHALG_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN2586979.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            15131..17314
                     /locus_tag="ACHALG_001584"
     CDS             15131..17314
                     /locus_tag="ACHALG_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFN2586980.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            17375..18595
                     /locus_tag="ACHALG_001585"
     CDS             17375..18595
                     /locus_tag="ACHALG_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFN2586981.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            18592..20052
                     /gene="gtfA"
                     /locus_tag="ACHALG_001586"
     CDS             18592..20052
                     /gene="gtfA"
                     /locus_tag="ACHALG_001586"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HFN2586982.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            20129..21235
                     /locus_tag="ACHALG_001587"
                     /pseudo
     CDS             20129..21235
                     /locus_tag="ACHALG_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            21557..21766
                     /locus_tag="ACHALG_001588"
     CDS             21557..21766
                     /locus_tag="ACHALG_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFN2586983.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            21759..21908
                     /locus_tag="ACHALG_001589"
     CDS             21759..21908
                     /locus_tag="ACHALG_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFN2586984.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            22062..22727
                     /locus_tag="ACHALG_001590"
     CDS             22062..22727
                     /locus_tag="ACHALG_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN2586985.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(23011..24096)
                     /gene="xerS"
                     /locus_tag="ACHALG_001591"
     CDS             complement(23011..24096)
                     /gene="xerS"
                     /locus_tag="ACHALG_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HFN2586986.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYITNEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            24430..24663
                     /locus_tag="ACHALG_001592"
     CDS             24430..24663
                     /locus_tag="ACHALG_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HFN2586987.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            24660..25067
                     /locus_tag="ACHALG_001593"
     CDS             24660..25067
                     /locus_tag="ACHALG_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFN2586988.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(26204..26722)
                     /locus_tag="ACHALG_001594"
     CDS             complement(26204..26722)
                     /locus_tag="ACHALG_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HFN2586989.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            27089..27337
                     /locus_tag="ACHALG_001595"
     CDS             27089..27337
                     /locus_tag="ACHALG_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2586990.1"
                     /translation="MVKLKMLYIGLQHRSFFDCALGKKYYEVKIDDDTFLTAHQMKQL
                     QKRNDQLEENLILKKRLPYSRLARRTIKGQDLPEDHPF"
     gene            27840..28677
                     /locus_tag="ACHALG_001596"
                     /pseudo
     CDS             27840..28677
                     /locus_tag="ACHALG_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612146.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
BASE COUNT         9865 a         4360 c         5267 g         9185 t
ORIGIN      
        1 attttgctcc acttttagat aatttttagg tctatttaac tcatcagaca ttctatgctc
       61 atttttctcc aaatttgttt caactctttc gtacttaatt ttgcgtattt gaaaattctt
      121 cttttagagt acttttatta cgatactagt atgatatata tctatagcaa acagttaaaa
      181 tcttcctaaa ataagcgaat agcattccta aaacctataa aatttttaga tgaagcgaca
      241 agtggacaat taataaacta cttgaacatg aaattatgga tcacctttca aatttagaaa
      301 atggaattct tatacttatt gcgcaacatt tattgattgc aaaattatgt gacaaaaaaa
      361 actagtagaa gaaagaataa tggcttgata ataaagtact acctattaaa ggtagtactt
      421 tatttaattt agtataaatt aacatactac tatattttag gtttcatatt atataaatta
      481 ataatatact accatttgtt ggtagtataa tttaaaggta ataagttatt cttttattac
      541 tatgtttggg ttttatagca aagaaagttt aataagagtt tggacctagc aaacaatatt
      601 aatgaggtca ttaaatctta ttatctttag tgatataacg aaatatctac acagacaaaa
      661 tgatagcgta aaatattttg tgtaaataga tatttagggt agaaaaaaag aaagtccatt
      721 ctgtaaaatt aaagttacca caaccgaaat tttataggag gactttctca tgcccaattt
      781 tactacagaa attatggaaa cactaatcaa taaaggtgat ttagatgaat tatttcgatg
      841 tcatttagaa ttagctgtta attcattatt gcaggctgaa ttaacagctt ttcttgatta
      901 tgaaaaatat gatcgtgctg ggttcaattc aggtaattcc cgtaatggga attattcacg
      961 ttcattcaga acagagtatg gagaattaaa tttggtaatt cctagagata gaaatggtaa
     1021 gttttctcaa caaactttac ctgcttataa aagaaccaat gactctttag aaaccactat
     1081 tattcagcta tttcaaaaag gaattactat gtctgaaatc tctgaattga ttgaaaaaat
     1141 gtacgggcat cactatacgc cacaaaccat ttcaaatatg actaaaatag tttctgaaga
     1201 tattattgcc tttaaagaaa ggtccttaga atccagatac tctgtcattt ttatggatgc
     1261 tactcacatt cctttaaaaa gacaaactgt ttcaaaagaa gcagtttata ttgtaattgg
     1321 cattcgatta gatggaacca aagaggttct tgggtttagt cttgctccaa ctgaatctgc
     1381 ttatgtttgg aaagagattc ttcaagacct aaaagaccgt ggtttaaaag aggttttatt
     1441 agtcgtaact gatggtttaa gtggcattaa cgatagtatt catagtgttt atccaaatgc
     1501 tcaatttcaa caatgttgtg tccatatatc tagaaatatc gctcataagg ttcgtgttag
     1561 tgatcgacaa gaaatctgta gcgatttcaa attagtttat caagcaagtt caaaagaaga
     1621 agcaaataat caaataaggt ttatgataga taaatggaaa aagcagtatc cacgagtggt
     1681 taaattgctc atgaatcctg ctatattaac cttttataat ttccctccgt caatcagaag
     1741 aactatttat tcaactaatt tgattgaagg ttttaacaaa caattaaaga aatataccaa
     1801 aagaaaagag caatttccta atgaagaatc tctggagaga tttctagtat ctcaattcaa
     1861 caactataat caaaagtttc tatgccgagt tcataaaggt tttaaagaaa tacatgatac
     1921 attagaatca atgttttaac ttgatcaaca gaatgggctt ttccatttac acaaaattat
     1981 tgacgctccc gacaaaatta tgatcttgcg taaagttata aaatattaat aaaaatattt
     2041 tattaatgaa aagcttggta gcacggttat tcatatgcta atttatttca ctttatagta
     2101 aggggagttc ttagtatatt gttcaaactt aaatttcata taatttaatt atgtaaatta
     2161 gctattctaa gaacttctac ggataactgc ccgtctaaaa tttaaataag agcttagaat
     2221 cttcatataa gtaaggttaa tttttttgta caaaaaagtc ggaatattct gagttactga
     2281 aatgatataa aatcataaac taaatccctt gattttattg actttcaaaa gtttatatat
     2341 ataagttaat gattatggta aaatatatac ataatcatta actcagaata tttttaagaa
     2401 gggaaacaat agtgagctat aatgtacaac cgttaaggac aaatgaagag atacaagatt
     2461 ttcagttttg gctaagaaga acaagtaatc cggaaagaga tagtttttta tttttgtttg
     2521 gaatcaataa tgggttacgt atgtcagata ttataggatt aaaagttgga gatatacgag
     2581 gcaaaagtaa gcctattatt gttgaacata aaacaggtaa gcgaaaaccg atttttatag
     2641 ataatttgag agaagaaatt ttattatata ctgaaggtaa agaagaaaat gattggttat
     2701 ttccgagtcg gcaacaaggt cgacatataa caagggatcg tgtctatcag atatatgcag
     2761 atatagcaga aaaattagga cgagatgata taggaacaca tacattaaga aaaacttttg
     2821 ggtatcacta ctacaagaaa actagagaca ttgctacctt aatgtttatt tttaatcata
     2881 gctcgcaagc aattacaaaa aggtacattg gtataactga agatgaaata ggagcatcac
     2941 ttcgtggatt taaactaggt gtataattta aaaattttta taaacagtaa agacaaagat
     3001 taaaagctat ttttatctag aacgatttta tttcataaag ttcgcggaat aaaagctggg
     3061 gttactggta cttggtatag gttcgtgtta tataaactaa gtaattaaat aacattttta
     3121 ggttggaaaa ggatgaaatt aaaaaaagag aatcaatacg gtctcaaaaa cgcaaacgct
     3181 gcggaataag aaaaatactg ataactcaac gtttagcaga ctatctaata gggatagtct
     3241 gcttttttcg tttttgacca tacttttgac cttctgcaaa cttccttgct tttcctctgt
     3301 agaactcatg aaatctgcaa aacgaattgc cgtttcctct gtctgctttt tcgcgatatg
     3361 ggtgtatata tcatccatag tggttttaat atctgtatgt ccaagtcgaa catacacttc
     3421 tttgacagac gcaccagatt caaagagcag actacaatgg gtatgacgaa aaccatgaag
     3481 tgtgatatgt tttaagttat tctctttaat gatgattttc aaattgtgat ttacatagtc
     3541 taaataaagg ggcttatttt cttctgatga aaacaaaagt tgttcaccat cttttatagg
     3601 tttgattctc atttttagat attatgcccg cacgtattca tcacatttct attgtgaatc
     3661 gctttattcg accgacattt gacttttatc ataatatact cggtttaaaa ttactaatga
     3721 aaacaattaa tcaagacgat cataccatgt atcacctgtt tttttcagat aatcaccacc
     3781 gtgtgggaac tgaactgact ttttttgaag tacaagaagg gaacaaccag ttttttggaa
     3841 ccaatacgat tgaacgaacg atactaaaag ttccttccga agcatcattg catttttggg
     3901 aaatatattt tgaaacacaa ggcgtttgcc attatggaat cgaatcattt tctggacgac
     3961 ctatcttacg ttttgaaggg ccagatgcaa cacgattagc tttagttccc ttacgcgaat
     4021 ttgaagatat tgatgattat ttcccgtatg tcacagacca gatccctaca gagcatgcca
     4081 ttttaggaat tgatgccatt caactacgcg tacaatattc cgatgcgact gaacgagagt
     4141 tattgtcggt aggctggtag cacaaagaat acgtcagttt cttttcgggt catcaacgag
     4201 tgacaatctt agaaaatcaa gacactcagt tctatcaaga agttcagatt attcaagatc
     4261 gtgagaatcc agttgccatt gaagggattg gtagcgtcca tcatgttgcg tttggcgtag
     4321 aaaataaatc tgatttacaa cgcatcgaca aacaattaca agagcgcaat tttataaatt
     4381 caggaataaa agatcgtgaa ttttttgtat cactttatta tcgtgaccca aatcaattat
     4441 tgattgaaat tgctacaggg gaaggaaacc tagatgcaaa agcgtatgaa aatcaaagtc
     4501 cacgctttga agaaattcct ttatttttac cgcagtactt aaatttcatt cgtgaacaag
     4561 tggagcaata attagctaat caaatccatg acgaagagta agaggttgac tcataagtca
     4621 aaaaataaag aaaaacgcat caaatccatt ctaaagaatg ttgatttgat gcggttccta
     4681 tttttataaa ctatcatttt cgactaataa aactacttat gagtcagcct ctcttttttt
     4741 cttgattttt atacctcttt ctaggtaaac taaacatctg ccaaaaatac cgtgtctcat
     4801 tgaaacattc tcaactattt caaaataaag gggagtacca aatgaacgag tatattgaaa
     4861 ttatccacgc aagagaaaca acttaaaaat gtcagtctca aaattcccaa aaataaaatt
     4921 agtattttta ccagcgtatc tggttcagga aaatcatcga ttgtttttga tactattacc
     4981 caagaagctg gtcgtcaact gaatgacacc tatgatagtt ttacccgttt atttttacct
     5041 aaatacagtc gtccagaggt tgatgagatt gataatcttt ctacggcgat tgtggtcaat
     5101 caaaagaaga ttggtggcag tgcgcgctca acgttaggga cgattactaa catcaatgcg
     5161 ttatttcgtg tgttgttttc atgttttgca acacctagtt taggatatgc aaatgcttat
     5221 tcatttaatg atcctagtgg aatgtgttct gtctgtcagg gaatcggtaa gacgattgca
     5281 tttttaatag gattgcttta tttttcggct attgataatg ctgattttgt attttctgat
     5341 ccatttaccg cagaagtttt gtctggaatt gaggaacagg caaagaatca tggttatttt
     5401 gtattggtgc ataatgttaa ttcagcagaa gatgttaaaa ctattcagag aaattggcgt
     5461 tttgaggggt tcattgttgt tggtgtacgt gtaaaagaat ttttggaatt aaataacgtt
     5521 tttgatggtc ctgttgttta tattgatacg catctttcaa aagagacatt aagagaactt
     5581 gaacaacaaa tgaatcgttt atttgtaaat acaaatgatt accaagctag tacattggca
     5641 gtggaacatt tgattcatta tggtcatgag aaaatagcgt ttctttcttt tgactacatt
     5701 gtaggtgaac caagtgttat tcaagaacga catcaagctt atatcgatac attaaagaaa
     5761 tatcatttaa cctttaaatc gcattggata tataacaact ctgaatttga gcgaatttat
     5821 caagaattag ctgaatttac tgcaattgtt gttactggag attatttagc ggctaaatta
     5881 gtaaaatttt taaaaaataa gaataaacga gtgatggaac aattatcgat tattggattt
     5941 gatgatatta cttttgcaga attaatggat cctgcgttga caacaatacg actaaatcca
     6001 ggtaacaagg gggcgattgc ttttcaccaa ctgattggac tggccaaaca agaagaaata
     6061 aaaaatcaag tactactatt agagggagaa ttgatttctc gagactctgt tcacaaaatc
     6121 aaataaaaaa gctctgtgaa ataagtatag ttaacaataa aatatccgaa cgatagagag
     6181 aatgaactgt gatctagcag ttcctcgtcg caaattcact agaattagca ttatttactc
     6241 taaattgttc ggatattttt tcttgttttt aattatgatt acagtgccta tttttaacga
     6301 aatttgctta ttttcaaaat gtatgattat atgttagtaa acactttcaa atatgaaaag
     6361 aggagctatt atggtaggaa ttagggatgt tgcaaaacgg gcgggtgtgt ccattgcaac
     6421 agtatcgcgc gtgctaaatg aggatccgac actttcagta accgatgaaa ccaaacgaaa
     6481 gattgcagaa tcggttaatt tttatcatta tgtcaaaaaa agtcccgttg caaaaaattt
     6541 aacaaatatt gcattaataa cgacagtttc tgaagtagat gagttggaag atccctattt
     6601 tcgaacgatt cgcagaggga ttcaagtcga agcagaacaa agcaaagtca atttgaagaa
     6661 aattattcgt ttgtcagaaa caccgttgga gcaagacgat ttgctaaaat gtcaaggtgt
     6721 gttgattatt gggcaggtgc tatcttctgt gattgaagaa gtatcagcaa ttaattcgaa
     6781 tgtcgtagtg attgatgacc ctagtattga agctgaagta gatgcagttt atactgattt
     6841 aacaaaagca acgaatcagc atttagatcg attgtataaa aaaggacacc gaaacattgc
     6901 gtttatcgga ggaggacgtg ttcgtttaga tagccaagga aataaacacg tgtcagatga
     6961 tgattatcga aaaatagcct atgagacgtg gatgaaacaa aaaggattag aaaaatatag
     7021 tcaaatttat ttagaaggct ggaccacact tgacggaatg caggcagcag atttactttt
     7081 gaaaaaacat gctaaagata agttgccgac tgctatcatg gtaggaaatg atcctattgc
     7141 tgtgggggtt tatcgttcac ttcaaaaaaa tggtctacat attccagaag atatttcgat
     7201 tgtgagtttt gataatattg aagttgctga atttttaaca cctgcattaa atacggtgaa
     7261 tgtgaataca gaagaaattg gaaaactagc agttcgcatg gttaatgaac gaataaaaga
     7321 agttagaaat gtttcgatca aagtgattgt ttctaataat attattgtta gagaaagtga
     7381 aaaaaatatc gattaaaaaa gtaaacatcc aaaaaagggt gtttactttt ttgtttacta
     7441 atatttttag taaaaaatct cgacaaaaca ttaagtaaac agttatggtg ttgttaacaa
     7501 aaagaaaacg cattcaaaac aggaggaatt aagatgaaac atttcaaaaa aatagcttta
     7561 acagggatag ctgttttaac tgggacatta ctcgcagcat gtggtggggg agatagtaat
     7621 tctaaagacg ggcaagtaac tttaagctat gcgagttgag atactgacca agctccagga
     7681 ctaagaaaaa tgttagatga atttgaaaaa caaaatcctg atatcaaagt agaaatggag
     7741 acaacgcctt gggatcaata ttgggttaaa ttagaagctg caacaacggg aggtaatatg
     7801 ccagatgtag taaccatgca ctctagcgaa tcctataaat atatgtcaca gaatgctttg
     7861 atgggacttg atgatgttat caaagaaaat gatattgaca tgggaaactt tactgaagga
     7921 attgctgatt tctatacgct tgaagacgaa ctatacgcaa tccctaaaga tgcttctgta
     7981 gtggggctat ggtacaacaa agaattattt gataatgcag gtgtcgatta tccagatgaa
     8041 acatggacat gggaaacact cagacaagct gcaattgatt taacagatga atcaaaaggc
     8101 atctatggtt ttgcatcaca tattggaaca gagactggct actggccatt tatttatcaa
     8161 aatgggggag aagtatttgc cgatgacaat acaaaatcag gtttgaatac cccagaagca
     8221 caagaagctt tagcttttta tcgagatatt gcacttgttg atcatgcttc accaacaatt
     8281 acagaattac aagatcctga taagttaagt cgttttcaat caggtagatt agcaatgatg
     8341 attgaaggaa actggtttgc accgggattt ttgaaaaatg actatctagt tgaaaatgtt
     8401 gcttctgccg tgttacctaa gggaaaagtt aatgcaacaa ttacaaatgg acttggttgg
     8461 gctgccagtg cgagtacaaa atacccagaa gaagtgaaaa aactaatggc atttttagct
     8521 agtgaggacg ccaataaaat ccaagcggaa acgggggcat caatcccagc actaaaaggc
     8581 tttgaagacg agtgggctgc ttcttcagat acaattgatt tgagtgcttt tggaaaaatg
     8641 ttggagtatg gagtccctcg tccatacaat aaagatggat tacgagctga acaagcagaa
     8701 gttcaaatta ttaaccgtat tctgtctggt gaagtaactg ttgaagatgg aaccgctcaa
     8761 attgaaaaag aagtcaatgc gatattgagc ggaaattaaa ttagttaatt atacaatata
     8821 caggcgaaag gattctattt cgtctgtata ttaatggatg aataccaaaa agggggatat
     8881 tatgaaaaat cgaatttttg gaaaatatcg taaagatcaa gtgatatggg gctatatttt
     8941 tatttttcca acggcattag gattaatttt attaaatata tggccagcaa ttcaaacagc
     9001 aattttaagt tttcaaaaaa cggtagggtt cggtgatact gaatgggtcg gattaagaaa
     9061 ttaccaaaaa atgtttgcag acggtgaagt ttttcagtct ttattaaaca cattaattta
     9121 tgcagtggtt tcgataccta gtattgttgt attatccttg ttggtggcag ttttaatgaa
     9181 tagaaaagtt aaaggattgt cgatttatcg gacaatttat tttctaccag tcgttgctgc
     9241 accatccgca gtcgcaatga tttggcgctg gatgttcaat aatgattatg gactgattaa
     9301 taacatgttg gctaaaatag gtctcgatgg accagcatgg ttaacggatc catcgattgc
     9361 gatttattct attatcattg taggagtttg gagtgctatt ggttataaca tggtgttatt
     9421 actagccggc ttataagaaa ttcctaaaga ttattatgaa gcagcggaaa ttgatggtgc
     9481 tagtccaatc cagcagtttt tcaacattac gttaccatta gttacaccaa cattattttt
     9541 tgtagttgta acaacagtta tcaatgcttt ccaagtattt gatgttattt ttatgatgat
     9601 tactcctaat agtaccgcca tgtttaaatc acaatcatta gcctatttat tctataaaca
     9661 ttcatttatt ttaaatgaca agggatatgg ttcggcaatc gttatgttcc ttttagtgat
     9721 tatattaatt attactgcaa ttcaaatgaa gttgcagaaa aagtgggtaa actattaagg
     9781 aggctatcaa attatgaaaa ataaattttt ttcaaatgcg ccaatccata tcatgcttat
     9841 tttaggtggt attgcaatgg cattaccttt tatttggatg attttaactt cagggaaaac
     9901 gttggttgaa tctacccaaa ttccacctac aattttccct gagaaatttc agctaagtaa
     9961 ctatcaacaa gtacttgatt tattaccatt catggctttt tatatcaata catttttaat
    10021 ggttgttgga cgagtgattg gctcggtttt attttcagca atggctgcat atgcttgtgc
    10081 gcgtctaagt tttccaggga aaaatatcct tttcagctta gtacttattc aaatgatgat
    10141 tcccggttcc atttttatta ctccacagta tttattagca caaaaattgg gtattttaaa
    10201 tacaattcca gcattaatat taccaggggt agttagtgcg tttggtacgt ttttattaag
    10261 acaatttttc ttaggtttac ctgatgaatt ggaggaagcg gcctatttag aaggtgccaa
    10321 tatttggacg atctttttca aaattatgtt gccgcttgct aaatcaggat tggttgcact
    10381 tggaatattc acagcgttgt ttgcatttaa agatttaatg tggccattga ttgtcaatac
    10441 ttcaattgat aagatgcctt tagctccggg attagcaaac ttacaaggac aatatgccac
    10501 gaacttccca caattaatgg caggttcgtt attagcaatt tggccaatag ttgtgatatt
    10561 catcgttttc caaagaaaat tcattgaagg aattgctaca acaggtagta aattataaaa
    10621 ctattaatta gtgattggag agtaagtaca tgacaaaaat tacatttatt ggtgcaggaa
    10681 gtactatttt tgcaaaaaac gtattaggcg atgcgatgtt aactccaagt ctacaagatg
    10741 cacatattgc attgtacgat attgatgaaa aacgactaaa agaatcaaat ttaatgctta
    10801 aaaatattaa taaaaattcg aatgaaaatc gtgctgatat tgtttcttat tatgatcgca
    10861 aagaagcgtt aagagaggct gattttgtta tcaatgctat tcaagtgggt ggttataaac
    10921 cttcaacagt gattgatttt gatattccta aaaaatatgg gttgcgacaa actattggtg
    10981 acaccatggg gattggtggg atttttagaa ccctacggac actaccagta atgttagact
    11041 ttgctaaaga aatggaagaa gtttgtccaa atgcttggtt attgaactat acaaatccga
    11101 tggcgatgtt aaccatgggt gttttaaaag cgacgagtat taagactgtt ggtttatgtc
    11161 atagcgttca agtatgcgta ccagaattgt ttgagcattt aggaatagcc gatcaatatg
    11221 acttaaatga ctttcaatgg aaaattgcag gcattaatca tatggcattc ttgttggaaa
    11281 ttacaaaaaa tggggaagat ttctatccaa caattcgtca gttagctgca gaaaaaccaa
    11341 atccacatcc agattctgta cgttttgaat tgttgaaacg ttttggttac tatattacag
    11401 aatcaagtga acacaatgca gaataccatc catactttat taaatcaaaa tacccagaat
    11461 tgattgacga attagggatt ccgttggatg aatacttacg tcgttgtgaa caacaaatta
    11521 atgattggga aagcatgcga gaggacattg taaataatgg ggagttaaca catacgagaa
    11581 gccgtgaata tgcttcatat atcatggatt ctattacgac aggaactccg tcagtgattg
    11641 caggaaatgt cttaaataaa ggcttaatca caaacttacc gacagatgtt tgtgtggaag
    11701 taccttgttt agtcgataaa aatggcgtgc aaccaaccta tgtcggtgaa ttgccaacac
    11761 aattagctgc tttaaatcgg acgaatatca atgttcaaga actgacagta gaagccgctt
    11821 taacactaga taaatcaaaa atttatcaag cagcgtattt agatccacat ctatcatctg
    11881 aattgtcttt aacagatatc aaaaatttag tggatgattt aatcgaagca catggggact
    11941 atttacctga atataattaa aaagaagctg aaagagtaag gaattttcaa ttccttactc
    12001 ttttcttagt taaatttagt aaaaatattg acaaaacact tagtaaacag ttacattagt
    12061 gttaacaaaa agaaaacgca ttcaaattgt ataaggagga gcataaaatg aagtggtcta
    12121 aaaaaattat tttagcttgt atagcactag gagcaacgat ctctttgact gcttgtggaa
    12181 aaaatcagga agatgcaagc ggtaaggtag aaatagagtt ttttaatcaa aaaaaagaaa
    12241 tgactcaaac gattcaggag attgctaaag attttgaagc aaaaaaccct gatattcatg
    12301 taaaagttgt ggatgtaccg aatgctggtg aagtaattaa aacacgtatg cttgcaggcg
    12361 atgtaccaga tgtcattaat ctttatcctc aaagtataga attgaaagaa tgggcgaaag
    12421 ctggttattt agaggactta accgaagaac cgtatttaga gaatatcaaa aatggatatg
    12481 cacaacgttt tgctattgag gatcgtgtat atagcatacc attaaccgct aatgtctatg
    12541 gtttttacta taacaaaact gcttttgagg aaatggggat tcaagcacca gaaacttggg
    12601 cagaatttga aaaaatagta gctgagatta aagatcaaaa caaagtaccg tttgcgattg
    12661 caggagcaga aggctggact ttaaatggtt atcaccaact agctatagcg actgctgctg
    12721 gtggtgaaga ggaagcgaac aatgtttggc gtttctcaga agttaatgga attaatgctg
    12781 aaagcaaaga aatgcaaaaa gattttaatc gattagattt gttaagagaa ccaaaagctt
    12841 tgcaaaacaa ctggcaaggt gccggttata acgatacagt agttactttt acaaaaggtg
    12901 atgcgttgat tatgccgaat ggttcatggg caatgccgat gattcattca caaaatccag
    12961 attttgaagt aggaactttt ccttttccag cagatgaagc aggtcaaagt ctaacgattg
    13021 gcgcaggaga cctagcctta tcagtttctg caacttctca acataaagaa gaagccaaac
    13081 gttttgtaga atatatgaca acacctgaag cgatgcagaa atattatgat gtagatggtg
    13141 caccgtgtgc tgttgagggc gttatcgaga atactcagga ttcaccatta agtggtttaa
    13201 ctgaacttgc ttttactgat cgtcatttag tttggttagc caaagattgg aatagtgaaa
    13261 atgattttta taccttaaca acaaattatt tacacaatgg caatcaaaaa gccatgatta
    13321 acgcattaaa tgctttcttt aatccaatga aagctgacgg aaggtaggaa aataaaatga
    13381 aaataaaaga aattttaaat cgttactggg caatcatttt tgtagcaatt ccaattattt
    13441 tgcagatgat ttttttctac tttccaatgg ttcaaggtgc tttttacagt ttaactaatt
    13501 ggactggtct gacttataat tttgattttg tgggtttgaa caattataag attctttttt
    13561 ctgatccgaa gtttatcaaa tcgattggct tcactttaat tgtgacgctt tgcatgattg
    13621 ctggacaaat tggttttggt attttgattg cgcgggcgtt gaacgcaaat attaaaggga
    13681 aaacattctt tagagcatgg ttcttttttc cagcggtctt atcgggcgtg gttgtttctt
    13741 tagtatttaa acaaattttt aactatggtt taccaaaaat tggtagtatg ttaggcattg
    13801 aatttttaca aaccagtctt ttaggaacaa catggggagc gatttttgca gtaatttttg
    13861 ttttactttg gcaaggaatt gccatgccag tcatcatttt cttagcgggt tttcaaagta
    13921 ttccacaaga aattattgaa gctgctagaa tggatggggc gaatgataag caaatttttt
    13981 ggaatattga aatcacctac ttattaccaa gtatttcaat ggtatttatt atggcattaa
    14041 aaggtggctt aacagcattt gatcaaattt ttgctttaac tggtggtgga ccaagcgatg
    14101 ccacaacatc tttggggtta cttgtttata attatgcttt taaaaacaat cagtttggtt
    14161 atgcgaatgc tattgcttta gtcttattta tcttaattgg gattgtttca ctggcacaat
    14221 taaaattatc gaaaaaattt gaaatctagg aggtttccca atgaaaacat caaaagaaaa
    14281 aaatatttct aaatacttgc ttttattatt agggggcttc atgattttag ttcccttagt
    14341 ttcaacgcta ttcggttcat ttaaaacaac acccgatatt atgaataatt tctttcgttt
    14401 tcctaatcca gcgactttgg aaaatttccg aagattgtta gcggatggaa ttggtcatta
    14461 cttttggaat tcggcaatca tcacaatcat tgcagtagtt gctgtgacag caattattcc
    14521 tatggctgct tattcactag caagaaaaat gactcgtggg aaagcatacg gattgatgta
    14581 tttattttta attttaggaa tttttgttcc tttccaagta attatgattc cgattaccgt
    14641 tatgatgagt aaaattggtt tagcaaatat gtggggatta atcttactct atctcactta
    14701 tgccgtacca cagacactat ttttatatgt tggttttatt aaactaaatg tgcctatcag
    14761 tctggatgag gctgctgaaa ttgatggggc tagtcacttt acaacttatt tcaaaattgt
    14821 tttcccaatg ttaaaaccaa tgcatgcgac gactattatt cttaatgctc tatggttttg
    14881 gaatgatttc atgctaccat tattaatttt aaatcgtgat tctaatatgt ggacactacc
    14941 attgtttcaa tataattata cgggacaata ttttaacgac tatggtccaa gttttgcttc
    15001 ctatacagta ggaattgtca cgattacact agtttatctt gctttccaaa gaagtattat
    15061 agctggaatg agcaatggag cagtgaaata accaataaat tattctcatt ataaaggagt
    15121 tcataaactt atgattatat ttgacaatca cacacaaaca tttcatttac aaaataaaaa
    15181 aattagctat ctattaggaa tcgaagaaaa taattattta acgcatcttt attttggaaa
    15241 aaagattacg cactattctg gtgggtatcg ctacccccga acagatcgtt ccttctcacc
    15301 caacaatgca aatgcgcaag atcgtttatt atctttagac acattaccat tagaatttcc
    15361 aggatatgga catggtgatt ttagagaacc ggcgcataat attaaattag gaaacggtag
    15421 tcgtgtaaat gattttcgtt atgatagcta cgaaattcaa aaaggaaagc ccagcttagc
    15481 gggattgcca gccttatata cggaagatga tgatgaagct gaaacgctga ttattcaatt
    15541 gaaagacgcg attagtggat tgactttaca gttgttctac acaatttatc aaaatcacag
    15601 tgtaattatt cgttctacgc aattagtaaa taatagtgat gaaacagtgg agattaaccg
    15661 cttagctagt caatcaattg attttccaaa tcgtccactc gagttaattc atttaaatgg
    15721 tacctgggca agagaacgtc aaatgactag agaaaaaatt catacaggga taaaagtatt
    15781 agatagtaaa cgcggatcaa gcagccatca acaaaatccg tttgttgctt tagtagatgc
    15841 caaaacagat gaatttcaag gagaggcata tggatttaat ttagtttata gcggtaatca
    15901 cgaaacggtg attcaaaaag atccatttga ccaaacacgg gtcattatgg gaattaattc
    15961 atttaatttt ggttggcaac taaaaactgg cgactcattt caaacgccag aagtcgtgat
    16021 ggtttatagt aatgaaggtc tgaatacgtt atcacagacg tatcatgaat tgtataatca
    16081 ccgtttaatt cgtggaaact atcgtttaaa agaacgtccc acgttaatca acaactggga
    16141 agcaacgtat tttgatttta acgaagaaaa aatcatgaat attgtcgatg atgcgaagaa
    16201 cttgggcatt gaaatgtttg ttttagatga tggttggttt ggcggtcgtg aaaatgactt
    16261 cacttcatta ggtgattggg ttgaaacaga aggcaaactt gcttgtggtt tggaaaattt
    16321 agccaagcaa gtgcatgata acgggatgaa atttgggatt tggtttgaac cggaaatgat
    16381 ttctgaagat agtgaactat ttcgagcaca tccagattgg gcattgggta ttccaggtcg
    16441 tgaccgctca attagtcgaa atcaatatgt tcttgatttt tcaagacaag atgttcgtga
    16501 taatatctat caacaaatga cggcgatttt tgatcgtgta ccgattgatt atgtgaagtg
    16561 ggatatgaat cgaaatatga cagaagtcta ttctgtcttg ttggactcaa cgatgcaagg
    16621 tgaagtggct catcgttata tgttagggtt atatgaattt ttagaaaagt taacaacgaa
    16681 ttatccaact attttatttg aaagttgttc gggtggaggc ggtcgttttg atgcaggttt
    16741 cttgtattac atgccacaaa cttggacgag cgacaatacc gatgccgtgg ctcgtctgaa
    16801 aattcaatat ggaaccagct tagtgtatcc aatttcaagt atgggtgcgc atgtttcagc
    16861 agtcccaaat catcaaacag gaagagaaac gagtttaact attcgtggca atgctgcgat
    16921 ggctggtgtt tttggttatg agctagatct aacgcaatta acttctgaag aaaaagcaga
    16981 aattaaagaa caagttgcct tttataaaaa gcatcgccaa cttttgcaat atggaacttt
    17041 ttatcgcttg gaaagtccgt ttgaaggcaa tgatactgca tggatgtttg tctcaaaaga
    17101 ccaaacagaa gccatggtct tttatttccg agtattagca gaagctgcgc atccattagt
    17161 cacattgaaa ttagcaggat tagcagaaga agcaacttat caaatggaag atgtaacatt
    17221 tgctggcgat gaattgatga atctcggttt ttatattgac tcagaactac atggtgatta
    17281 tgcgacacaa cgcttttatt tgaaacagat atagtagctt gaatttacaa tataagtgca
    17341 acaccaaaag agactcattc caaacgaaga aaatatgttt tatttacacg catttactaa
    17401 aaagcagcct gatttgaatt gggaaaatga agaagtacgt gaagaaatct atgcaatgat
    17461 taactattgg ttagataaag gactaggcgg atttagaatc gatgcgatat taaacatcaa
    17521 aaaaaaacta gagtatggca tctttgaatc tgatggagaa gatgggttag ccttcattgg
    17581 gccctggatt cttaatcagt cgggcattga agtgtggtta aaagaaatga gagagcggac
    17641 atttaaacca cataatagta tgacggtagc agaagccgat gttcctaatg aacgtttaga
    17701 tgaatatatt ggtgaagatg gctattattc gatggttttt gattttagtt atacggatat
    17761 tgatgtacct gaaactggtg aatggtttaa agatagccaa tggacatgga cggatatgcg
    17821 taccaatatt tttacgaatc aattagttac gcaagataaa ggttggggcg ccttatattt
    17881 agaaaaccac gatcaaccac gttcaattaa taaatatatt ccagaagaat ggatcaatga
    17941 ttatagtaaa aaaatgttgg cgacgctttt tatgttacta agaggtaccc cttttattta
    18001 tcaaggacaa gaactaggaa tgaccaacat agaaatggca tcattagaag attttgatga
    18061 tgtagctacg cattctcagt ataaacgggc attagaatat ggattggcac cagagcaagc
    18121 attgaaagct gtcaataaac gaagtcggga taattctcgc acacctatgc aatggaatac
    18181 gcaaaaaaat gccagattct caacttctga taatgtatgg ttaaaagtaa atcctaatta
    18241 tccagaactc aatgcagaag ctcagttaac caatcaacag tcggtattca attattatca
    18301 tcaattaatt aaattaagaa aaaatagtgt atacaaagac gtgctaatct acggacaatt
    18361 tctaccagtg aaagataaaa atgaacatat tttgctttat gaacgccaat tagaagataa
    18421 aatcgtatta gttatcttaa acgttacagc aacagaacaa gaatattatg tagatgaaca
    18481 gtaccaacaa gtattaatca ataattatga agatgtttta ttagatcaaa gtcgaaaatt
    18541 gcgtttacga ccgtttgaaa gtattgtatt agctaattat ggaggacaat tatgaaaatt
    18601 aaaaacgaag caatgctcat cacctatgca gatagtttag gaaaaaacat gggcgaatta
    18661 aaacaagtct tagacaaaca tttacaaggt gttattggtg gtgtacatct gttgcctttt
    18721 tttccatcga caggtgatcg aggctttgcg ccgagtgatt atacgacagt tgatccagct
    18781 cttggaacat gggaagatgt ggaagcatta ggtgaagatt attatttgat gtttgatttt
    18841 atgattaatc acatttctcg tgaatcaaaa tttttccaag atttcaaaaa aaaccatgag
    18901 cagtcaccat acaaagaaat gtttatccgt attcatgaat ttttcccaga aaatcgacca
    18961 acacaagaag atattgattt aatctacaag cgtaaagaca aagcgccgtt tcaaacggta
    19021 gaatttgcgg atggtacgac agaagaggtt tggaatacct ttggggaaga acaaattgat
    19081 ttagatgtga ccaaagaagt tgtgaaagaa tttattcgag aaaccatcaa agatatggcg
    19141 aaccatggct gttctttaat tcgattagat gcgtttgctt atgcgattaa aaaattagat
    19201 accaatgatt tcttcgtgga accagatatt tgggaacttt tagacgaagt gcgtcaagaa
    19261 gcagcgaaat atgacgtcga attattacca gaaatccatg agcattattc cattcaaatg
    19321 aaaattgcca atcatgatta ttatatctat gacttcgcct tgccaatggt cactctgtat
    19381 tcactttata gtggaaaatc agaacgttta gccaattggt tgaaaatgag tccgatgaag
    19441 cagtttacaa cattggatac gcatgatgga attggtgttg tggacgctcg tgatttatta
    19501 acagatgaag aattggatta cacctctgag gaattgtata aagtcggggc gaatgtgaag
    19561 aaggtctatt caagtgccaa ttacaataat ttagatattt atcaaattaa cagcacctat
    19621 tatagtgcat taggcgataa tgaccgtagc tatctgttag cacgtgcgat tcaagtattt
    19681 gcgccaggga ttccacaaat ttattacgtt gggttattag caggaaaaaa tgatattgaa
    19741 ctgttggaag caacgaaaga agggcgtaat attaatcgtc attactatga tttagccgaa
    19801 attgaagaag aagtgcaacg gccagtgatt caacaattat ttgcactttt aaaatttaga
    19861 aatgaatctg cggcgtttga tttggatgga acgattgaag tagcaacgcc atcagaatca
    19921 gaaatagtga ttacacgtaa aaataaaaac ggtagtgata gtgcaacatt aacggctaac
    19981 ttggatacga aagaatttat cattgagtct aaagatataa aaattttcac tagtgttgca
    20041 ttaaaatttt aacctattcc atagaacaaa aaatccttta tactagcact tggatgactt
    20101 atccaagacc aataaaaagg actcatacat ggataagtat aaaacaaata cagcatttaa
    20161 taaatggttt tctgccataa aattaggaaa actttctgtc aactcccaaa aatgtatttt
    20221 tgattacaat tcgtatcgta aaaaactgtc ttttgaagct gtgctcaagc tctttctcta
    20281 cgcagttaat gaagaaaaag aaagtttgcg tgatttgagt acagctctta tcgaccgatc
    20341 gctccaacta gaaaccgatg tgtcatcgat tagtcatacc caattgtctc gagcatttaa
    20401 tgcacttgaa cctaaagtgt tagaagaaat ttttcaacaa ctgttagaaa aagttcaaca
    20461 tcaaatcaaa ccaaccaagc gaaatcatct gtatctgatt gattccagta cgttttcgct
    20521 tagtttaaaa cgtcataagt gggcgacttt ccgtcaaaca aaatcaggtg tcaagttaca
    20581 cttaaactat tgttatatgg acgatactac gatgtatcca actgatttta ccttaacaaa
    20641 tgcccatgaa catgatctca atcaactacc agtattggtg aatcaaccag aggcaactta
    20701 cgtgtttgat cgtgggtatt aggactttga aaagatggat cagatgcatt gggatggcta
    20761 tttcttcgtc actcggatta aaaaaaacac caaagttcac gtgatagata ctctggaaac
    20821 atcgccagaa acagaaattc tacgagacga gctggttcgt ttaggatcta aaacctatct
    20881 aaccgccaat ttccgactag tcactgttca agataagaac ggacgtgtgt ttcaatttat
    20941 caccaatcga atggacgtct cttcaaaaga aatctctgat atgtatcacg ctcgttggca
    21001 aatcgaattg tttttcaagc acatcaaaca acacatgacc attaaaacct tcttttctca
    21061 aagtgaaaaa ggcgtgcaga atcaattgat tttaacgatg atttccgctc tcctcacgtt
    21121 tctcatcaaa ttggagacga aaacggaaaa atcagtgttc caaatcaaac gattctttcg
    21181 ttatttactg tttcagccct ttgaatgttg tctagaaaaa ttaattccca catgagaaaa
    21241 aagaaatttg gctcgttact tttggttttc agttgaataa cagataaaaa attttctgga
    21301 aaaagtcatc ctctgcgtca gcacagtttt ttttgactta ttttcagagg aaatagtttt
    21361 cagaatattc taaaatgttt tatgcaacac tagtgatatt ttttagaatt agatttctaa
    21421 tgtgcatttt ttgagtatta aggagcaagt atacaaagat ttataaagct gattaaattt
    21481 gaataaaaat atgcaggaac gctactattt ttagtagtgt tcttttttat atttgaataa
    21541 aaaatgtggt ggttaaatgg tggttataac aaaaaatgaa ttaatgaaat taggttatgg
    21601 gaagtatcag tctgagagaa tcatacgaca ggcaaaagca attatggttt caaaaagcta
    21661 tacctattac gaaaatagaa gattaggact agtacctgtt gaatctgtag aagaagtttt
    21721 aggtatgaag atagaggtag aggaggattt tcagaatcat ggttaatttt aaaaaagata
    21781 aaaatgggaa atatcatttt actgctagtt tgggattcga caaacgaaca gggaaaagag
    21841 tacaaaaaaa cgtagagggg ttaaaacagt taaagaagaa aaaaagcgta taccgaactt
    21901 gtttctaatt atggaaaaga agcagaaact tctcaaagta ctatgttgtt tgaggagttt
    21961 ttttataaat tattcctacc ttattatcaa gggaaagtag aagaaagaac atataaaaat
    22021 cggttatcta caatcaaaaa aacttttctt atttctttaa aatggagtta agagaaatac
    22081 gtccaattga cattcaaaag tggcaaaata atttattgag caaatataaa aattcttatg
    22141 taagaaacat gtatgggatt tttcagatgt cattagatcg agcagttgtt ttgggactac
    22201 ttgaaaaaaa tcctgcaaaa gttattggaa atgtgaagaa aaggaaaact acagttgatt
    22261 tttggacaaa agaagaattt acaaaagtta tttcaacttt ttatattgat gatttgtacc
    22321 aacattttgg ttttatttgt atgtggttac tttttatgac cgttatgcgt ttgggagaag
    22381 ccactgcatt aacttggaaa gatattgatt ttgaaaataa aaacttgcgt gtgaataaaa
    22441 cactctatta taagagagcc aaaacctatg agtttgtaga acccgaaacg gcttctagta
    22501 ttcgtacaat cgaactagac gacaatacaa ttaccttttt aaaagcttgg aaggagcgtc
    22561 aagtttcaat aggaggcatg tcgtttgtac tttcttataa tggaacacct actcagaaat
    22621 atacaatccg tcatattatg agaagacatg caacgatcgt tggtgttcat ataatccgca
    22681 tacacggatt aaggcatagt catgcttctt tgcttatatc acaataaaaa gatgcacttc
    22741 gaattaaaga aaggttaaaa catgctgatg tacaaacgac tttaggaact tatggtcatt
    22801 tgtatctaaa ttcattgaga gagataacaa atgatcccac agattatatc caaactaatt
    22861 tttctaaaat caaaccgaca cctatttacg ataataaaaa ataaatgata aacaaaagta
    22921 ggatcacacc aaaagaaaca cactagaaaa actataatac aggcttttct agcgtgttgt
    22981 ttcttatttc cgcctattta tacattatac tcataaagca tttaatgcat cgatcgtagc
    23041 cgcattgtct atatgggtat ataaagttgt tgtattggta cttgaatgtc ccaattgttg
    23101 ggcagttaat accaatgaat ttgtctgttg gtagagtcta gtagctaatg tatgacgcag
    23161 tttgtgaggg ctgacttgaa ttctaaagcc ttttgaatat tttgctacca tccgttcgat
    23221 tgcaataccg gatatacgct tggcttctcc ttggtaaatt gctagaaata aagctttttc
    23281 gttttctgtt actttatatc gttctttccg aatagctaga taattagcta aatattccat
    23341 tgcgaatgct gctatattga cagcatctct tttatttcct tttctagtta ctaccacatt
    23401 attcttatct aaagataggt catctaaatt aatatttata acctcggaga gtctcaatcc
    23461 agatcctaaa atcagtgcat tgatcgccag gtctctttct acatctcgtt gataatagat
    23521 caaagctcgt ttactcaaag tatttttata ttcattggta atataatcta gataattttg
    23581 tgtatcctta tctagaaata attttttctc aatggcattg gcacgtgcag ctaacgtagt
    23641 tttatctgtg atggttgcta cttgatccat aggattattg tctaagtatg gttttcctag
    23701 aggattattg gaacttttcg aaagatattt aaatagaacc tttaatgccg tgatatttct
    23761 attgatagta gaagtagata gacctaagtt attattatct ttgagcttag tttttgttgt
    23821 ctcttttttt acattttctt ttgtgcgacg tttcaaatga gatttgtaca agtttaggtc
    23881 ataggcagtt aactgtctta tcgaggtgat agaagtatca ggaacgatag actcactcag
    23941 aagccattca taaaagagct tataattttt tagatattct aaagtggttc gaggtgattg
    24001 atctttatcc tcacgatctt gaacaaaatc ttttatataa tcgggcatat gaagtatgat
    24061 tgtatctatt ttttgatata tttctgtatt tttcatgtca ttcatccttt caataagtag
    24121 gggagaagag tatctctata attggactat accaattata gaatgttttg tcaattggta
    24181 tgctctaaaa tttattaatt tgtttcaaaa aaagcatata aacaggtata cgtttaataa
    24241 tttaaaataa atggtataca taaataggga aggaaataga agggagcaat ctaaattttt
    24301 tgaattttct taattaatta tatagaaaga acaatctttc tatagagtta gacatgagag
    24361 ggactatgct tgatttaaaa tcaacaagcg tatatactgt atatacaaag aaaaaggagg
    24421 gagaagacta tggcaattaa agaaagaaaa tttagggaag tggggaactc agtagtatta
    24481 actatttcaa aggagtttct taaagaaaat gggttaaatc ccggagatac ggttttttta
    24541 gatgaagaaa aacttcgagc ggcaattaca aaaaaacaag ccttatctga tgaaattgat
    24601 ttagcaatat ctcaagcatt aactgaatat gatactactt ttagagagct tgtcgataaa
    24661 tgagtattca ttacctagaa gccaaagata ttataaaaat gaatgtaatc caaataaaaa
    24721 aatactctcc taatgaaccc cttggtgtaa aggatgcgaa tgctttagaa atgtgtgtag
    24781 ggcaacttcg agcctcagct tttggtgaag aggtttatcc ttcgatttat gaaaaagcag
    24841 ctatcctact tatacaattg ataaagaaac atccttttca caatgcaaat aaacgtactg
    24901 ctttcttagc tacttttgtc tttttgaaaa tcaatggaca gcttttagct attgatcaaa
    24961 aagatgctgt aaatttagcg gtatatattg ctacgtatga taaggatttc gatcaactaa
    25021 aatatgaagt gatcaacact ataaagacac atacgcagcc catttaagga gttactgtat
    25081 gtgtctttat agggttatta gctgctatag cgaaataatt gaagtaaatt acaaatagct
    25141 ataattcaaa tccaactata tagaaaacgt aaattttcta tatagttgga ttaagataac
    25201 gactatttat tttatcatat ttttactata ccctatactt ttacttggta tatttatgaa
    25261 taatcgcaaa tttaccccta tactactagt ttgtaagtta gattaataaa aatactaaag
    25321 ttatttgttc ttttattaaa tacacctaca acttataaat tgatggtatt tagataacac
    25381 gaaagaataa aaataacagc tgatagtaaa gtttgttgac gattgctact caatcgaaag
    25441 tgagttagtg cgtatgaact tgcttctgaa aacttatgaa atgaaagacc ttttacatct
    25501 tgcagaaatc gttactggga ttaaactaag caataaaaac taatgttccc ctcattcttt
    25561 gaacttctac tatttgattt atttattctg ataaatgacg atttcacctt caggatcttc
    25621 aaatcccgta tagaatttat ttaagatctc ttcagtatca gtaataggat aggcgatagg
    25681 ccacataccc tttgatggtt agaataaact gctcaaaatc tgtatcgtat gttggcttta
    25741 gcatattaac aaaggctatc tccaaattat aaaccataag cgttgaatcg ttcttaacaa
    25801 tttcaattct tcctaaccca aagtcagaag atataattac cggacttgcc ttacccttca
    25861 tgtgggttgt tgagattcca tatttaaagg gaattgtcgg tactatcggc ggtctgaaaa
    25921 acatcccaaa aataaaaaaa tctttcccct gctagtctcc taagtccctt tgtagcggat
    25981 atggcaccat aatctcattt tatcgaagtg agtcatggtt tatctgttgg ccaaccaaaa
    26041 ttgatacaat ctaacgatgc accctctacg gcattctagg ggagtgcact tgcattcacc
    26101 caataaaaac cggcagttag caaagaactt atgtagtcgt atgttcttac tgttaacata
    26161 caagaggtct cctttccaca attgcttagc agtaaacggg aatttacaaa gctaatctct
    26221 tccattttct tatctttgtt ctaaaagtcc caaatggagc aactgtattt acatgaataa
    26281 atttatatac ttcccataca gccgttttgg ttgcctcgtc tgcccatttt cttttgtgtg
    26341 cagtgaatag atcttcatca ctcattgaat caatcatcat gttaatatca tcaatattac
    26401 gattgagaat ttcctctaat tctttcaatg gtagatgtgc ataggtattt gtaaaccact
    26461 tatataactc tcctaactga ttccatttaa attgatcaga aggcgtttta acagataatc
    26521 catttctttc atcattttcc cattttagaa ttaaattcgt ccaaccaact tgatatgcta
    26581 aattttcagc aggtgttcta tccacttcct caactctttt atccttcaat tcttcaggaa
    26641 tagtttcaaa ttctaaaata tatttagtat aagtcctatt tatttctttt tttaattctt
    26701 ctttattttt atatgttctc attatattac ctccataaat tcctaattgt ctcctttaat
    26761 tcatctataa tttcatccac gatattttaa tccgttctac aatgcatatc tgatatgcat
    26821 tgactgtcta actcatatgt gaatgattta taaaatatcc caacgtctca tctaaagaaa
    26881 tgttgatcgt cagcaaggag ctaaaaaata cgaatatatt tcttttgtag taaaatcgga
    26941 ctggtttgca tatcattttc ttcgtaaata tcaaaatcat gttaactact atcaattact
    27001 aataaaaact accactttaa gcgttaagaa ctgttaagtt tagttaagtg aggatagttc
    27061 acaaaatcca ttgttaaact tcacgaaagt ggtaaaactc aaaatgcttt atataggatt
    27121 acagcatcgc tctttcttcg attgcgcgtt gggtaaaaaa tattatgaag taaaaattga
    27181 cgatgacacg ttccttacgg ctcatcagat gaaacagcta caaaaacgaa atgatcaact
    27241 cgaagaaaat ttaattctaa aaaagcgatt gccatattca cgcctcgctc gacgaactat
    27301 taaaggtcaa gatttacctg aagatcatcc tttttaggaa attgaagctt tttatgttgc
    27361 acaatgcatt tataatacta caatgatgtt ttcaccagat attcttctag ggtgcggtgt
    27421 aatgaaacag atacacttgc aaaagaaaat tcaccgcgaa tttactaaaa ttatgaataa
    27481 ttatgttaat cttccgaaat ttttttccaa aattaaggga tcaagctggt gttatagatt
    27541 gtttaaggtt ggcaaaaaaa taacttcttt ttaaacaatt atagaaaacc gatactctgt
    27601 taagttggtt gacacggtgt cgatttttat gactttctta ttctgaaact tcactaacgg
    27661 acagtaccta aaaagtctat agtatggacg tttacgagta ctattttatc aaaaatgctg
    27721 ctcatttcaa cttatatgtt ttagtgaact acccccactt agcttggata ggctacaatt
    27781 aactagacag aaaaattaag gtgtgtagac tagaagaaaa cataccagga ggaattttta
    27841 tgtctaagag aacacgaaga actttttcac aagaattcaa gcaacaaatc gtcaatcttt
    27901 acttagctgg aaagccacgt gtagaaatca ttcgagaata tgaactaacg gcttcagcat
    27961 ttgacaaatg ggtaaagcaa tctaaaacga gtagttcatt caaagaaaaa gataatctta
    28021 cgcctgaaca aaaagaattg ttagaactac gtaaaagaaa ccagcaatta gaaatggaaa
    28081 atgatatttt aaagcaagca gcgctgatat tcggacgaag agacaagtaa tcgatgcgaa
    28141 taagcatctt taccctatat cagcgatgtg cagaatatta ggtctatcac gtcagtccta
    28201 ttattatcaa tcaaaaccaa agaaagacga atcagaactt gaagaagtag tcgctgaaga
    28261 atttatccgc agccgaaagg cctacggctc aagaaaaata aaaaaagcct tatcaaaacg
    28321 aggcattcag atcagccgac gaaaaattag tagaatcatg aaaaatagag gattaaaatc
    28381 gagctatact gttgcttatt ttaaagtaca tcattctact tgcaatgaag ccaaaacgac
    28441 aaacgtattg aatcgtaaat tcttaagaga caacccatta gaagcgatcg taacagactt
    28501 gacttatgta cgagtcggga aaaaatggaa ttatgtctgt ttcattttgg atctgttcaa
    28561 tcgagaaatt ctcggctatt cttgtggaga acataaagat gccgttctag taaaaaaagc
    28621 atttagccgt atcaaacaac ctctgacaga ggttgagatt tttcatactg atcgtgg
//
LOCUS       DAZABD010000035        27885 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000035 DAZABD010000000
VERSION     DAZABD010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27885
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.035"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(424..1368)
                     /locus_tag="ACHALG_001597"
     CDS             complement(424..1368)
                     /locus_tag="ACHALG_001597"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFN2586991.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1664..2608)
                     /locus_tag="ACHALG_001598"
     CDS             complement(1664..2608)
                     /locus_tag="ACHALG_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFN2586992.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2829..4043
                     /locus_tag="ACHALG_001599"
     CDS             2829..4043
                     /locus_tag="ACHALG_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFN2586993.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            4065..6080
                     /locus_tag="ACHALG_001600"
     CDS             4065..6080
                     /locus_tag="ACHALG_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HFN2586994.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(6163..7536)
                     /locus_tag="ACHALG_001601"
     CDS             complement(6163..7536)
                     /locus_tag="ACHALG_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFN2586995.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7562..7846)
                     /locus_tag="ACHALG_001602"
     CDS             complement(7562..7846)
                     /locus_tag="ACHALG_001602"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFN2586996.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7848..9863)
                     /locus_tag="ACHALG_001603"
     CDS             complement(7848..9863)
                     /locus_tag="ACHALG_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFN2586997.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(10042..10347)
                     /locus_tag="ACHALG_001604"
     CDS             complement(10042..10347)
                     /locus_tag="ACHALG_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HFN2586998.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10436..11467)
                     /locus_tag="ACHALG_001605"
     CDS             complement(10436..11467)
                     /locus_tag="ACHALG_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFN2586999.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11674..12549
                     /locus_tag="ACHALG_001606"
     CDS             11674..12549
                     /locus_tag="ACHALG_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFN2587000.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12701..13543)
                     /locus_tag="ACHALG_001607"
     CDS             complement(12701..13543)
                     /locus_tag="ACHALG_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFN2587001.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13635..15512)
                     /locus_tag="ACHALG_001608"
     CDS             complement(13635..15512)
                     /locus_tag="ACHALG_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFN2587002.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15509..16930)
                     /locus_tag="ACHALG_001609"
     CDS             complement(15509..16930)
                     /locus_tag="ACHALG_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFN2587003.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16933..20322)
                     /locus_tag="ACHALG_001610"
     CDS             complement(16933..20322)
                     /locus_tag="ACHALG_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFN2587004.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20579..21970
                     /locus_tag="ACHALG_001611"
     CDS             20579..21970
                     /locus_tag="ACHALG_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN2587005.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(22043..22690)
                     /locus_tag="ACHALG_001612"
     CDS             complement(22043..22690)
                     /locus_tag="ACHALG_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN2587006.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22698..23384)
                     /locus_tag="ACHALG_001613"
     CDS             complement(22698..23384)
                     /locus_tag="ACHALG_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN2587007.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23390..24463)
                     /locus_tag="ACHALG_001614"
     CDS             complement(23390..24463)
                     /locus_tag="ACHALG_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN2587008.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24685..25359
                     /locus_tag="ACHALG_001615"
     CDS             24685..25359
                     /locus_tag="ACHALG_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFN2587009.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25364..26704
                     /locus_tag="ACHALG_001616"
     CDS             25364..26704
                     /locus_tag="ACHALG_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFN2587010.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26853..27839)
                     /locus_tag="ACHALG_001617"
     CDS             complement(26853..27839)
                     /locus_tag="ACHALG_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HFN2587011.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         8133 a         5268 c         5053 g         9431 t
ORIGIN      
        1 aaaaatgata aacctctctc gaacggtttt ttatcaaaaa tcgtcatttt tccatcaaaa
       61 tctgcttatg caaaattata aaaaaatttt gcagtttttc ttactttttt tgtcgtttaa
      121 ttgaatgata aaaaccagtt tctttattca gttaatcaaa tatgaacgaa ttagatatga
      181 atcattgtat ttgccgttgc ggaaggattt tttaattaac cacagataaa cattgataac
      241 gattcgctta ttgtaatcaa cagattaaat ttcatatgtt catatgtacg gatacatttt
      301 cggaccgatt aattttaacc attctgttta tttgcttacc tattagatat caataaaaaa
      361 ctgcttaaca gctatttcag ctgttaagca gttttaggat ttttagttca tattcattgt
      421 ttcttataat acagtgttca atacttctct taaagcactt gttgatttgc tgaacattgc
      481 tttttcttct tcgttgattg ataattcaac aacttcacga acaccatttt cgtcaacgat
      541 tgatggcaca cctgtaaaga tatctttttc gtcgtattca ccagttaaat aagcagatac
      601 aggcaataca gcttgttcgt tattcaagat agcttttacg atacgtgcag tactcatacc
      661 aataccatag taagttgctt ttttacgatc aatgatctca taagcagcat tacggacttt
      721 atcagcaatc acatctaatt catcttgggc aatacgatgg tctttttcta caatttcaaa
      781 cactggttta ccaccgacag ttgtatgtga ccatgcagca acttctgaat caccatgttc
      841 acctaagata tagccgtgga cgctgcgtgg gtcaactttt aatttcaatg cgatttcttt
      901 acggaaacga gttgtatcca atgttgttcc agtaccgata acacgtgatg ttggcagacc
      961 agattcgttc caagcgatat atgtcaaaat atctactggg tttgaagcaa caacgataat
     1021 accgtcgaat cctgatccca tgatttcttt tacgatttgg cgcatgattg acgcatttgt
     1081 tttaactaaa tctaaacgag attgaccagg tttttggttg ataccagctg tgataacaac
     1141 gatatcagca tctttacatt cttcatagcc acctgaccat acagccacat tttcatcgcc
     1201 ccaagccata ccatctaaaa ggtccaatgc ttccccttct gctttttctt gattcacatc
     1261 gatcaatact aattcattag agattccttg gttgatcatt gcataagcga tacttgttcc
     1321 aacaaaacct gtaccgacga ttactacttt acgacttgtt tttttcatga ataaacagct
     1381 ccttttcata actatttgtt actttcatga aaagtatagt cttgtttatt cattttatca
     1441 acatgttaat tataatagtt gttattataa tttttgatgg aaacgaattc ctgaatacgc
     1501 ataacagata aatgacaaat gtttacacct atatcccctg ataatacaac caaaagttta
     1561 gaactgaaaa taaatccaaa aaaattcgct gcaacgatat aataagacag caatcgcacc
     1621 taaaacaatt ttccttcata tgctttagtg aaagattttc ttcttaatgt ttatttctcc
     1681 attcattggg agtacatgtt gaatacttct tgaataattt gaataaagaa gtcgtttgtt
     1741 tatatccgat acgattagcg atttcttcca acgaaaaact cgtttcttga atcagttcca
     1801 acgcaaagtc gaattttttc tttagaacca gttcttgaaa ggaagcatca aaatacattt
     1861 tcaatttatt actaagatag accggattgt agccaaaatg agctgctgtt tcttttaacg
     1921 tacaagaagt aaaatgctta tcgatataat ctaatactct aagcatctca ttttctgcgc
     1981 atccatctgc tgatgaaaag ctttgttgtt ctaataaatg agtcaattcg atcagtaata
     2041 gtgatgtata gagatttaac gaacggatct tatgactgta tgtatcaaaa taaatcaaca
     2101 gcatattttc aatcaaatgc gtcaaacgac ttgtttttcc gccgcgaaaa tgaatgaatt
     2161 gtgtatgttc ccctttttct tttgatgcat ttatcaaaaa agaagaaagt agattatttg
     2221 tgcttgccaa atgattcaat atctctgtag ataaggtttc ttcttttaat aagatgttga
     2281 ttaaaatatc ctcttgccct gtgtattcga ttctttgagt agcttcttta tcaatgatca
     2341 ccacgtcact ttcttccatc tctatccttt ctccattaat aatttgtaaa catgtaccgc
     2401 tatacatata attgatttca acaaaatcat gtgtatgctc gggaacttcc gcatatcgac
     2461 ggtgtttact gatatgaata tttccctgtt gaaatagatc ccctcggtgg atttttggcg
     2521 gtgtctgaag atggtaagct aagtttaaat cattgatatt cattccttgt tcacgctgca
     2581 gagtttcgat tgattcttcg ttacgcaaaa aaaatgtcta attcagctag ttccaattga
     2641 ctccctcctt tttgtaagcg gtttctttcg cttattagtt cacgctgtac tgctattata
     2701 tgcaaagcta tttttggttt cataaagact ttctatggtt gggacatgaa cttcctgtga
     2761 ttccgctatt gagagaataa ccacttcata ctatattttt gataagcgct tacaaaaatg
     2821 aaaggaacat gcttatgaat aaaaagatga tgaaattttc tctgctgtca gtctctctta
     2881 ttttgacttc tgctggtgca gtcacgtctg caattcccag tatgtcagct acttatgcca
     2941 cgcgttcttt atctgctgtt gaattattaa cgactgctcc ctcttttatg gtcactattt
     3001 ttgttttgat tagtagtttt attgctagga aaatcggaca aaaacaaact gtgatcatcg
     3061 gattagtgat tgcatcgatt tgtggtatcc tccccctctt ctcacaaagt tacagtattg
     3121 tcttagcttc aagagctgga ctaggtattg gctttggatt gatcaattct ttggcagtca
     3181 gcatgattgc tgccttttat cacggagatg aacgagcctc tatgattggt tttcaaagtg
     3241 catttcaagg atttggtgca gctttaatga catttgttgc aggacagcta gtacagttcg
     3301 gttggcaata taccttttta gtctacgcca tcagcttacc tattcttgtc ttgtttgttt
     3361 tgtttgttcc tacacctgaa acaactgaac ttcaatcgca tggcacagcc ccaacaagac
     3421 aagtaatcaa aacgaatcct aaaatgatta gttatggtat ttttctcttg ctggtcatcg
     3481 tggtatataa tgctgtatcg attaaattag ccacagtcct gacgacttat tctctaggaa
     3541 acgaaactaa tgcagcgaat atcttaacaa tcatgcaatt ggcaagtatg gctgccggag
     3601 tttgttttgg ctggcttcag acaaaagcac atcgctatat gcttacactt tctttactac
     3661 tgatgggaat cggctttgta ttgattgcca ctgtgataaa cctgtatgtg attgggtttg
     3721 gtgcgatcct aacaggaatt tctttttcgt tattcattcc ttacttattc aaccaagtaa
     3781 gtatccaagc gcccaagtac gcggagagtt tcaatacttc aatcttattg gttggcgcta
     3841 atttaggtac tttcattgca ccatatggtt tgctctttct ctcttttgct tctcttggga
     3901 gtaaaacact tgctgttttc gtcaatggca gtatcttgct gttgattagc gcgcttatta
     3961 gccttctagt agtacacagg acgaatagaa agacagtgat aagagtagat gaagaaataa
     4021 aagaacaatt gacaacaaaa taaatttgat ggaggtcgtg taaaatgaca aatgaaaaac
     4081 ataaagatag atggctttgg tatcctggag actttgagat ccgccatgga ctgctacaaa
     4141 atttccaacg ggaagaacga ggttttgact ggccagcgta ctggtacatg gatgattgcc
     4201 atcggaacgt caagtttaag agatattact ttcttgatca accttccatg tttaaagtga
     4261 cgatccaagg agttgggtat gtagaaatta atggacaaaa acatccatgt ggaaaatggc
     4321 tgacttgtcc agctggtaaa gcaaaaattc gtatctttgt aggacataca tcagggctac
     4381 cagcgatgtt tatcgaggga gacgaagtaa aatcagatat tggctggaca gcaagtaatt
     4441 ttattgaaga atatccagct ggctggagtc cgctttatgt cgatatcgca aaagatccaa
     4501 atcaaatcta ttatcaaaaa gaatgcgtcc atccagtcac tgaaacggag attggcggag
     4561 gaatcttatt cgattttggg cgagcagtga atggctgtgt agaactaata tctctggatg
     4621 accatatcga atctatcact ctatgttatg gagagtccga tgtagaagct atggatatcg
     4681 attattgcta ctataagcaa ataggaatga caagaaaaat gaaagctcgt aaacgcgctt
     4741 ttcgctattt gtttatacca gaggtttcta ctggaatggt tcaacttcaa gtttttcatg
     4801 aatttttatc tctttcgaaa caagctgctt tttcaagcga ttcttcttta ctgaatcaga
     4861 tctggtcagt atcagaagaa gccttcaagt tatgtagtga tttgtttttt atcgacggta
     4921 tcaaacgaga tcgctggatt tggtctggag atgcgtatca atcattcatg atcaatcaat
     4981 atcttttttt tgacgaggaa atcaataaac gaacgatatt agcattaaga ggacaaaatg
     5041 aaatcaagca gcatatgaat actatcgttg actattcgat cttgtggttg attggtatcg
     5101 aaaaccacta tatgatgacg aaagactatg aatttttacg catcgtctac cctaagatgc
     5161 aatcattaat gagatacttg atggagcaaa cgaatgaatt agggtttatt tatgggagag
     5221 caaaagattg gatatttatt gattggtctg aaatggataa agaaggaacg cttgctgcag
     5281 aacaagtctt attactgaaa gcctatcatt ccatgatcac ttgcggagat atcttagctg
     5341 aaacatatgg agagaacgaa aattatcata ctcatctcta tcaagaaaaa tatcaactat
     5401 tgaaacaaaa cctatttgac tacttttggg atgaagaact tggcgctttt atcgattgct
     5461 atgaatctgg gagaagaaat gtgacgcgtc atgcgaatat tttcgctgtt ttattcgatt
     5521 ttgctaacaa agaccaacag caatcgatct taaagaatgt cttattgaat gacactatca
     5581 cacagattac tactccatat ttcaaattct atgaacaaga tgctttatgc aaactcggac
     5641 agacgaatat cgtctaccaa acgattcttg attattgggg agggatgtta tctcatgatg
     5701 cggtaacttt ctgggaagaa tacgacccta ctcaaacagg aaatgaaaag tacagtatgt
     5761 atggagatcc ttatggaaaa agcttatgcc atgcatgggg agcaagccca atctatcttt
     5821 taggacgcta ttttatcggt ttgtatccaa caaagccagg ttatgaagaa tttatgatac
     5881 aacctaatct agattcattt gaaacactga attgtacgct acctatcaaa gatggaaaga
     5941 tttcgcttga aaaaacaaaa gataagatcg tgattacttc tgatagaaca ggcggaacag
     6001 tgaaaatgaa tggtaaaagt tatcctcttc tagcaaaaca gccacttgtc ttaacaagct
     6061 attcaaaaat aaatgcataa aacaaaaaac tgcttgcgac atatcagaag actttaaatc
     6121 ttcttctgat attgtccaag caatttttat tttgaagttt gcttaattct gttctttaga
     6181 aacaatgttt gttctttttc cggcaaaaag aaagctagat aatacgacaa gtaaaaagaa
     6241 agcgatgatt ccaactgtca aggctatttg accgtattct cctagtttcc caagtacaag
     6301 tcctggtact agataatcag tatcggcaaa tgtagaagaa gcgattttaa gatctcctaa
     6361 tactggaagg ataaataatg gcagccaact aatcaataag ctattgaaag cagaacctaa
     6421 taccgcccct cttctaccgc cagtcgagtt gccgaagaca ccagaagcag ctccgcagaa
     6481 gaaatgtcct actaccccag gaatgataac tgtagtttgc aagcctatca tagcaaacat
     6541 ggatagtgtt cctacaaaga agctaacaaa aaagccaatt aatacagcat taggtgcata
     6601 tggaaaaacg ataggaacat ctaatgctgg tttagaatcg ggtacgagtt ttttagcaat
     6661 cccttggaat gctggaacga tttctgccaa caccattcga accccttgaa ggacgataac
     6721 aaacccagct gtgaatgttc cagcttggat cagtgcaaag attacagcgt tgtctccatt
     6781 gcttagttct ctttcaacaa atccaggacc agctaacaaa gcaagtgcga catatacgat
     6841 cgacatcaag agcatgatcg atacagtaga atctcgcaaa aagctgaaac ttttaggaat
     6901 ctttatgtcc tctgtagaac ggtctttgtt tccaaaaaac tttccgatcc atccactaga
     6961 agcataaccg atatttccgg tatgccctaa cgcaacttga ttgtttccag tgatgttctt
     7021 cataaacggt tgacaaagag caggcgtaat agttaatagt gttccttcga agaccccgcc
     7081 taataagatc atccacaaat ttgcagtaaa ccccgcgctt actaaaatga ctgctgttag
     7141 acaagaaaca taaagcattg cttgacctgt aagaaaaatg aatttatatc ttgtgaatct
     7201 agcaagtaaa atattcacga tcatccctac tagcatgatc aaagcagtag gtgtaccgta
     7261 ttgttgcaaa gcaatcgcca ctactgcttc attgctagga actactcctt gagtatgcag
     7321 agctgtttgg aacatcgaag caaaaggggt tagtgaatta ctgattaaac cagcaccgcc
     7381 gatcagtact aggaatccga caaatgtttt gatagttcct tggatcactt ctgaaaattt
     7441 cttcttctga agaacgagac caagcagtgc aatcaaagaa acgaggatgg agggctgact
     7501 gacgattgac acaaacaaat tcaacaattt atccattgat agttcctccc ttgaatcatt
     7561 tttattcttt tgacaagatc ttcgttactt tttcttttac ttcttcgctg tcaatgatcg
     7621 attgcaaagg tacgagcttt tcatcaggta tagaagttag cgtatctttc aaagaatgct
     7681 caatgaagaa ataatcagcc atatcgctag atacacttcc aacatccgta tgacttactt
     7741 caaactcgtc cacatctgct ccaagatctg caagtgcttg tttgatgttc atctctacca
     7801 taaaacttgt tcctaatcca ctttgacata ctgcaaaaat tttcatatca ttcgtctcct
     7861 tttcttatga tcgctaatat ttcttctttt gttgatgcgt gtaataaatc ttgaagattt
     7921 tgtttattag aaagtagctt tgttaaattt gccaaagccc gaagatgtgc ctcattatcg
     7981 atagcagcta aacagatgaa caaactaatg ggatgggctg gattatcaag cagatccaca
     8041 ggttgttgaa gtttcagcaa agacatccct acttcgttta cgccatcttc tggacgagca
     8101 tgaggtaatg cgatgtgatc gccaatatgg ataaacgcac catactgctc cacgcgctcg
     8161 atcatagctt tgatataact ttcttctatt ttattttctg ataatagcgg ctgtgcggca
     8221 aatgtgattg cttcttgcca gttttttggt tgatccgata attggatcat ttcttctgtt
     8281 aataattcag atagcatagg acgttgatcc tccttctttt ctaaaagttt gttcatttta
     8341 cgatttaata cttgatagag cttttcttcg tctacgcctt cttttaattc gatataaggc
     8401 agtagcgccg acaacaattc ttgggcagaa ggcattgaaa atcctggtat cagccaatct
     8461 ctttgtactt gattgatcag ctgatttttt tccaagtcag tcatgattgg atgcagaaca
     8521 tagagagact ttttcttatc agtaagtgtg agaggtacag tagaaaaaat aatatcgtaa
     8581 gaagtttcgt caacctcgtg taagcggtca gctgaattag tctctgtaaa aaagatcatc
     8641 gggaataatt gctgaagttc actttttaag atcaatgaag agctaatgcc acttggacag
     8701 acgatcacgg ctcttatttt tctatttctt tcttctgcgt ccgcttttct gatctctccg
     8761 ccaaatagta tagtgaaaaa accaatctct tcttcaggga tgctcttccc tattcgtctt
     8821 tctaacggaa gtaatgcttt tcttgtcatt tcgtagatgg aagcatattg atgcttaatt
     8881 tgatcgatca tcacattagg taaataaaaa gaataggaaa tacgaaaata ggcagggacc
     8941 aagtgataaa agacattcat caataattca cgaaacgaag caaattctac ggccatcagg
     9001 aattccatgc gatggatgac ttctgaagaa cattccagca aaaaatcaag tgcaggctcc
     9061 tggacatctc cttcaacgat cgatgctaag caaagagaga aaaatataat ctccgaattt
     9121 tgatgttcgg aatagccaag gccagtcaat acttgtcgac taatgtcagt gactgcaaga
     9181 gaagccaaca gttcagcatc ttttaccagt tccatggatg tttttccaat tcgaatggtt
     9241 gtcaaactca aataataagc taattcatcc attcgatttg gtgcgaatcg aagatctgat
     9301 agtgaaatcg ttttatctag caaatctcta actttggcgt aataatcaat ggaaagggtt
     9361 gcggttaaat acgtgagccc aaatcttcca gcaagtgact gcatcaaacg agcagtgcga
     9421 ttttttgctt ctcttcttaa tattgcttct gaccctagca gcctaaaacc agttttccta
     9481 gtatacgaca gctgacaatc ttccacttct aattcatttc ttatcttttt gatatctgac
     9541 aggactgtcc ctttagaaac ctgaagaaaa tcttgaaaat gaaaaacaga aacagacgtt
     9601 gcagtcgaat aaaccatcaa ataaagaaga tctcttcgct cttgttcggt atacacagct
     9661 tcaaatcgag taatatccaa aaatttttct gcccagtcga ttgctcggaa tttctttata
     9721 cggatacggg tatcatccac atattcggca aaaggagtta attgtttgat cgcctcgctt
     9781 gtatttactt cgatgatctc tgctgctttt tctagactga tttctcttgg tgttttcagg
     9841 agataactaa ttttgtagtt cacagcgctg ttcacctcct acttatattc tagtatggta
     9901 gcgcttttaa atcgaggggt gtttggtaca acttacagtt ggattttttt tacgactgta
     9961 ccgaacgatt tctatcgaat ggatacagaa aaaaagcagg tgaccaaatg tctcctgctt
    10021 ttatgaattg atttcgtccg atcaatgatg atcatgttta ttttttgagg tcagttcttc
    10081 aaatttttct ttgacatctt caaccgcttt ttcgatatct ttttcaaagt gattgggctc
    10141 attaggcttt gtttcctctt ctacagaatt tgaaccagat aaagctgttt ctttattttc
    10201 tgtttcacca gagtttgtgc cagctaatga ttcttctttg tcttctgttt caccagaatt
    10261 agttcctgct aaagccactt cttcttctgt tttaggggcc attgaacctc ttaatcgttt
    10321 gtgtttttca ccgtcataat ttgtcatgtt ggttcctcct agtagttcta tactttttca
    10381 tcgtacaata agacagttta tgcgtcaaaa aacaaacccg attcttacaa atatgtcata
    10441 tttttccttc tgattcttga atgcttttga ttccttcttc caaaattttc atttgtaaga
    10501 ttgtttcttc atctttgatg actttttctg cgccattaac taatgtctcg taaatccctt
    10561 cgtagacacg gctgtaatca ccaacctcgg aaattacttt ttcttcatga taactccctt
    10621 gttcatctat ataggtcaat acaccataat gttcgggcaa atcgatacca aagtcttcat
    10681 gatcaggcat gtaaaacatt ttcagatgtt cttcttgtcg atcttttgtt tgtttcacga
    10741 acatcccttt ttttccgtaa acgacgaaac ttggacgttc tttgatgcgg aaataactgg
    10801 attttatcga aattttcata tttccataga aaaaatccag atcgaagtaa tcattcattc
    10861 gtcctttccc taaaagttga cgtacatcat agctgatatc atctggttcg ccgaaataag
    10921 aaagaacttg atctactgta tgacaagcat gtccatataa atagctgttc gcagtagaaa
    10981 attcatgcgt attttctggt acttccggtc tgaaataatc ataatgcatt tctacttcta
    11041 aaatatcccc taattttccg ctttcgatta ctttttggac tgtcaagaag tcagaatcga
    11101 accgtctgtt ttggtaacat tggataaata gacctttttc ttttgctaat gcaaataact
    11161 cttctgcttc ctttgacgtt tctgcaaaag gtttctctac tagtacattc ttaccatgtt
    11221 cgagtgccat ttttccatat tcataatgaa attgactagg tgtagtgatg acaactaatt
    11281 ggatctcagg atcatcccaa agatcagaca gctgatcagt atattcgatg tcttcgaatg
    11341 ttttccacac tggttcagat ggggaaaaaa tttttttgat attgattttg ttcgtaagtt
    11401 ttcttgaaaa aggcaaatga tagcgatttg tactctttcc attgccaaga taagcgattg
    11461 taagcatgag tggtgcctcc ttttattgat gttttcagta tatcctagaa aagcgcagaa
    11521 agtatttata atagctattc tggtaaaaaa gttcagaaaa acacttgtta tcattcggtt
    11581 tggtgaaatt aatgttcaaa gtaaggataa aaatcaaaaa gatagtagcg gaaccaagtg
    11641 tttatcagta agatagaatt tggaggtcag atcatgctta ttgaagaaaa actaacaaaa
    11701 caagagttgt ttaccacgac agagaaacgg attgtcgatt acatccgtag gaatatcgaa
    11761 gctgcagtct acatgacgat tgaagaatta gcaaaagcta cgtatacttc ccattcagct
    11821 atcattcgtt tatgcaaaaa aatgggattc agcggattta aagaattccg tttcgagctg
    11881 gggcgggaag ttcatcaatt gattgctcag ttcaatcaaa cgattgatcc taattttcct
    11941 ttttcaagtg aagatagcac tcagcaaatt gcaaagaaaa tggcggattt atcgatccag
    12001 tcaataaaaa aagcacaatt gcagattgaa catgaagatt tagatcaaat cggtaaagta
    12061 ttgaacaagg cacagcgaat ctttcttttt gcaaaaggtg attctcaaat cacggcaagg
    12121 aaattccaga ataaaatggt caagttgaat aagtttttga tcatggctga agaatacagt
    12181 gattcttctt ggaatgcagc caatttgact agcgaggatt gcgccatttt tattagctac
    12241 agcggtcgca tccatcatta tgaacgaata atgacctatt tgaagcatgt agaggcccct
    12301 accttgctga ttaccggaaa tcagcattca gaaatggcca aacaggctga tatgtgtctt
    12361 gttatatcgc aggaggaata tgattttgct aaagtagcga ctttctcctc tcaaattgcc
    12421 ttcgattacg tattaaatac gttattctca gtgatatatt ctcaaaattt tgaagagaat
    12481 atccgcaact tgaaaaacaa acaagaattg atgcaaaaag gccttcttaa agaatatcgg
    12541 aaaacttaag aaaagatcct catttagtaa taagaaagat agtgtttgat aggctctctt
    12601 tgatatcatc ataaaaacag gctgaagttt tctttttcaa acctcagcct gtttgtttac
    12661 ggaacgattc gaatagatat attatagaaa tgaggaatag ctaacttctg tttcttattt
    12721 tgaacgattg acgaaaaaga tatataagaa ggaggaaaat cagtatacat gcagctaaca
    12781 atccatacag gaaaaatcgt ttgttctcgg atactttctg tacttctttt tgaacttctt
    12841 ttgcatgata aggaacccgg tgccctctga caagtaagcg atgactgttg accatataag
    12901 gtgtacaagt taggagtgtc acgaaatcct gccctttttc gattctcaga tcttttgtat
    12961 cagtcggttc taccgtcttg atctgatcga cttgataagc gagtgtccgt ccattgattt
    13021 cgatatagaa agaatcacct aaagtcagtt tcggtaagtc tgtgaacaac tttgcttgag
    13081 gcagtcctcg atggctggaa ataacggcat gtgtattttc tccccccgta ggataagacg
    13141 ttccttccaa taatgaggca cctttttcta gcagcacagt attcgtttca tcaaaaattg
    13201 gcaaatggac attgatctca gggatcgtca aaataccgat cgtatgtttc tcaaaatagg
    13261 ttctatcctt tttcgcaggt tcttttttct tagtaaaggg atctgcacct ggatttccac
    13321 cttcttttgc taattgctgg tttttctttg tcatgttttc ttggatttta gccatgactt
    13381 cttcattttc tttgaccgct tgttgctgat aatgagagat gatctgctga tccagataat
    13441 tattcagcgc gtcgctaaca aatggataag ctagtatccc tattcctgag agaagcaaga
    13501 aaagcatcag cagatctaga agtttccttt tctttggttt catttgtgtt ctccttgtga
    13561 tgactgtttg ataataaaag aaaaaggagc atttgcgtaa tccgctgctc ctttttccaa
    13621 ttcttgcaaa aagactaaat ctgactgtgc ttgcgtctag caaagtacag tccagcaatc
    13681 agtagaagga ctactcctgc accgatatac acatagattc ccttaccgcc tgttgaagga
    13741 agtgtacctt tgtgtttatt tggtattttt tcaggagaaa tcaactgtcc tgctgttaca
    13801 taagattgtt catcgatagt aaatgctaca cggtttgtta atggaacata tttttctggc
    13861 gctttcgttt cttccagata gtacgtgcca tattttagac ctgtcacatc gattaaaccg
    13921 ttacttgtgg ttgtaaaaac cgttgctgat tctttcgccg atacccagct gacggctttt
    13981 gtggatgggt cgatcgataa atatttcgct gtgtcactat cttgatcacg aacgacgaat
    14041 tctgctccag caagtgtttg gtctgatgta acgtcaccat ctactttaac aaatcgtttg
    14101 cccccagtaa cgacatcgac tgacggtggt gtttgatcat ttgtatgccc gttatcgaca
    14161 tttgcttggt tagaaaatcc tttggttgga tctgcttttt cgttcaaatg catatagtaa
    14221 acgaatttca atgtaccgcc aggagttaat gaaggaatat aattcggatc aactgaaacc
    14281 gtgaatccgt ctgtttgctc agtgacagaa taatttactg ggtcgatttc tttatttcca
    14341 tcatataagg cataagcata cgttcctgaa gaatcattat caaatgttaa agcagcgtca
    14401 tgagtatcga taagtttgaa tgttgtgtac ttgttttgcg ttccttcttt atcagcgata
    14461 cctaatggga tattgacaga aatctcatat tgaatttttt caccgattgc gacatctttt
    14521 ccattcaatt gaggtgttgt tttatcaact ttagacgtat catttttgat ggtcttttct
    14581 actggtgttt ggctatttgc caggatctca aaaggcgtga ttgtttgctt ttcaatcatt
    14641 tccgcattat caggagcttt tacttcttct aaattatatt ttccaacttc taaatgatta
    14701 acgatcaact ggcctttttc aatagatgcc tcggcaaagt cattgttgcc gatgtcataa
    14761 gaatgaccag taatgaaatg tttggctttg gtttgatcag ttgtccaagt gtacaatcca
    14821 tctgtgacac tttggatgta ttttttgacg cttggtgttc cttcttcttt agaaataata
    14881 aattctgctc catttagtgc ttcgttttcg gcagtaccga tttttgtaac tttcaacgtt
    14941 ccatcattac cgactgtatt tttagggtag agatggatag tatctagttc ttctgtcccg
    15001 tatttataag agccatctgc ttgtttgatc atctcatata cagggaaagc taaaaccatg
    15061 tttgcggcag ctgacactcc gtcttttggt tcttctttga tcgtgtagac tgcatctttc
    15121 ccattttgtt ttttaggtaa agataaagtg acatttccat cagcatctgt cgttcctgaa
    15181 gcaactggtg taccaggagt caaagactgg actgcttgtt ttgctgcatc cacggacgct
    15241 cctttatctc gttgcgcata aaattcttga gtgacgttat aaactgaaaa tgaaatatcg
    15301 gctaatcctt ggtattgatc gaattcgctc atttctttcc cgctgttttg gattaaagga
    15361 tcgggtaaat cagtcatttt ctttttatga aggatgactt gagctgctgt ctcctctgca
    15421 aacacttttt ttgcgccgaa gctgtttgtg agtaatggta aaataaggaa aaggactcct
    15481 aacataacgt ttattttttt atgatttttc attttttcac tcctgttctt tttttgataa
    15541 acaaccagat tgcaaagctt gcgaatacca taccaacaac gcctaaaagt gtatttttcg
    15601 tttcgtttgt ttttggaaga aatggacggc tattttcttt tttattgact actttcaata
    15661 tactgtccgc ctctttatcc tcagaaagaa aagttgaaat ggtaaacgga atttctgttt
    15721 cacttaaaat ataaccagaa ggtgcttcga tctcttttaa tcgataactt cctgttttca
    15781 acccggaaat tgaaaaagcg ccatttttat cggaaatcag gtgaagcgca tctgattcgt
    15841 tctttgtcca cttgtatccg ttggctgttt gctggaggta atttcctgct ttgtttttaa
    15901 taaggaatac tgcgccagct aatcgtttat ctggtttatt tgtctcgatt ttgacaaatt
    15961 gcttactccc tgtatataca gagactttct ttgtactgac accaaaacca aaatccagtg
    16021 ttccgttgtt gttgatttcc ttgttcgcct gtgctgtact gcttagctgc atctgataag
    16081 aaatggtcag tttttttcca gcatattttt ccaacgaagg gatcgaaaat aaaacatcaa
    16141 atccgtgagg tttcttgtgt atgacaaagc cttctgtcag cttttctcca ttcgatgaaa
    16201 tcgttaaact attaggtaaa aacgttaatg cttcatccgc actatctgac aattcgaact
    16261 tttgatagtc aaggatattt acaggaattg tcgtatgcaa ggaataagtg attttttcac
    16321 caatcgtaaa atcatttcta ggctcttcta ataccttctc aaaaggtgga tcagggtagt
    16381 cgttttcttc atttttagga tacaaatgga tagttgaaag tttttgattg ttttgtccat
    16441 gtacaggaag aacaactacc atattctctg ccttttcttt gacgacttct ggtactttgg
    16501 attcaatgaa aagatacgct ttatctcttt tttcttgatc tttagcggcc agagaaaaag
    16561 aagcgatacc atcttcgttg ttaagagttg tagttgttgc ttctgcggta aacataccgg
    16621 aagacgcacc gttttttgcg atctctgctt gtgcttcttc taccgttttg cccttttccc
    16681 gtagatggta aaaagaatct gtgacatcat aaacttggaa tgtgacacca tttaatcctc
    16741 gatacgtttg taataaagct ttttcttgtc cgtcatttgg atgatttttc ggcagttgcc
    16801 cgtttgggaa aagcaatttg tgtaaagtaa tctgaaccat ttctgtttct gtttctgttg
    16861 ctacctgagt aaaagctggt ttaaatagta acaagaagag acacatacta agccaaccaa
    16921 gttttttcat atttagctgc cccctctttc ttttttcaaa aaaagatacc aaatagaaaa
    16981 cgcacaacca atcatcccta ctagatagat tcctaaacgg ccaattcctc ccgtttcagg
    17041 taatggtact tttgcctgat tcgtgatgtc taaagaaatc tggttgtttt tggctcctgg
    17101 tgacagaaca gattcatgat cttgtccatc cacttgaatt gacccatctt cgtgtataac
    17161 tatagtaact ggctctttta gaccttggta tccttctggt gtaaaagttt cggtcagcgt
    17221 ataagttcca ggttttaaat tttcgaatag aaaggtatct gtttcttgtc catcttttgg
    17281 cgattctaag tccatttctg gtccctgcag tctgaatttt gctcctttta atgtctgtcc
    17341 gttgtcttct tttttgtgta ccgttagatc aaacgctttt agtcgatttt gatgagtgag
    17401 cgtccataca ttatttgtca tctcggtatt ttttcccgtg taaatgattt ttccatattt
    17461 atcgatttgg aattcataat ttcccggcga agtatcatat ccggcaggac cagctgtttc
    17521 aacgactcga tatttaccag gtgtgagtga atcaaaactg gcaagacctt tttcattcgt
    17581 tgtttggctg tcaattggct gataagtacc atttgcttct tttttctgta gggaaaatgc
    17641 tgctccttta agatttatct ctttgtccgt cccttgcatc gcatacttac gtactgctac
    17701 aggaacttca acaaaaggat tttcaatagt caactggatc gtatcatcgg aagtagtgac
    17761 tgtttttcca tcaatggtta ccgtaccatc agaagcgatc ttgatttccc aagtagtctt
    17821 tttgcttaat ccatgccctt ctggagcttt tgtttctgtc agcgtatagg tcatttcttt
    17881 ttgcaatttg acattttctg gaagagaata ggttccgtcg ccatgatctg ttagtaaagt
    17941 atcaatagaa tctcctgtta atttgaaaac agcgccaata agatcctttt cacctgtaga
    18001 ggaatttttc gttattttca agtttaacgg tgtgaattgt ttagtatttt tccatgtcat
    18061 tgcatctatt tgttgagaat cgtatcctgg tacaggtaat tctttgattg tttggtaact
    18121 gaatgcttga ccttgattat tgtattgagg tagtgataat atctcttgat aactttctcc
    18181 gctatttgca gataatttgt caatgtctgc acgttcccac gtatttgttg tatctttagc
    18241 tggtttaatg attcgaatat atccgttttt ccaagctgca gcatttgtcg tatgttcccg
    18301 ttgaatctca aaagtaactt gatctggacg atcagctaga ttgttgtcaa attcttccca
    18361 taactttttg atgtgaagac tgactccggg agctttagca gatggtatac cgaattcagc
    18421 taattcattg gtatcaatgt ttggctggaa agttgtcctg ccgttcattt gataccagaa
    18481 atttggatgg aagtcctcat tttctgtttg gattctcact tggtaatgga tttggatttc
    18541 ttggtctttt cctaaataga tctgattgct cttgatggta tttccttcta tggaaataga
    18601 tggagtgacc gttgtaggac ttgtccccac actcttgact gaaagtgtac caggctgata
    18661 aatgaatggt tctgcgattg gatcatttat ttgtccattg cttacagttg ctgagatctg
    18721 tacagctttt ttggctaggt attcagagat atctgttgca tgatcagctg attcatagta
    18781 aagatcgcct ttttcatctg atgaaaccat ttgtcgcata cgagactcta cttctgcttt
    18841 tgagagacca gctgccggat cgctttgaag ttggatgcca agaccatgta tttcgattcc
    18901 tcgttctttg agtgccatcg cttctccgat ggttgcgata aacgtactgt cgattcttct
    18961 ggatagattg ttttggtcag gtgcatcata gattcttgag attagagaag tatttcccgg
    19021 ccgatcttgc gtattagaaa actgagttcc gtaataattg ctgcttgatt gtgcgtgtac
    19081 ccgctgtact ttataggaaa atgttggtac accatccgtc agcaaaacga tcactttttt
    19141 atgtccattt ggaacggata gcatgcttcc tgcatctctt agtgcttttt gtgtaaaagt
    19201 accaccattt gtccgtgaag gtgtaatgga ttttacttga tttttcactg aatcaaatga
    19261 acccatctgt actgcaccgt tactgtagct atatccttcg cttgagtagc cgacatatcc
    19321 catattgatt ttgtctgtga taccgctatc tgctaaagta tcgacaaaac gatcgacacc
    19381 aatctttact tcaccgatcc gattattgtc gttcatactt cctgaccagt ctacgaccaa
    19441 tacgagatca agaggcgtga tatctttttg tacatttcca cgagcattca aataaacatc
    19501 aaacaatccg ggtgtacttg tttcctttgc atactttcgt atcgcatagt ctgcttgatt
    19561 accggttcct ccatattcga tatacgaatg ggtccgatca ttaggatctc cattccaaga
    19621 atttatacca tcccattgat ttgttccgtt tttattgcct tgatgatcaa gaacatttgt
    19681 atttcctgta ggttgccaac tggctttagg ataagtgccc tgatcatctg ttgtatattc
    19741 tgtaggaatc gtttgattag atggcacact cacagctggc aaggataatg taggcaatgt
    19801 aaatgggctg acagaatttt cgttttcttt tgatgattct tcttctattt cgtctggctt
    19861 tggtactgac acatctgtat ctttcttatt ttctgtctca tcaataatgt catattcttt
    19921 attatcgacg atcagcttgt ttgcctggct gtggtaaagt gctggatcga ttggaaaact
    19981 cagatcgata gtttgtttag tgtttgatga gatttccaac gtaattttcc cgctatttac
    20041 ctgataagca tagccatctc cttcttttct ttccacatca cttaagtaat tttcgactag
    20101 accaatttct ttttctatct tttcagcact ggtgttattc gcatttagct tgatgttcag
    20161 tgcttttttt ccagactgat ccgaaatgac ttcagctgaa attgtttcta aaactgttgg
    20221 atcatctgta gtgtctgcta ttgcagataa tggcacaaag tttgataaac tcaatattat
    20281 cagcatgaaa ataactttca gtttcttccc tgtggtcgtc atttttcccc tcctttttct
    20341 ttttttctgt tttgacgtga atagtgaaaa ttattaaaat aaacaggcgt tacataattt
    20401 tatttatttt aatatgataa ttattatacg aagggcgcta aagggaatca caccaatttt
    20461 cataccaatt ggtttcattt atgaagttga ttccttcgat taatgagcgt ttcattgaaa
    20521 gaaaagggtt gagggagaaa aacatatgat caggttgttg gaaggaaact atagaaagat
    20581 ggaaaaaatc attcgaattc tgtccatttc tgacaagtgg atgactaaaa aagaacttgc
    20641 caaacgaatt ggtagttcag aatctacatt tgtaaggtat atcgaagaga tcaaaatccg
    20701 ctggggaacg gatgtaacga ttcaatcttc acaaaaattg gggtatcgtc ttgaacattt
    20761 gaatgtacca gcttatttaa atgttctgat ggaaatgtcg caaagttcta caaacagtcg
    20821 tttattaaat gagattattg tcaatccggg taaaacgatt gagtattatt gtgaaaaaat
    20881 ttccatcagc cggtcttctt ttacacgtaa gttgaaacaa tgcaatcaaa ttttagaaag
    20941 ctatttatta aaagtgatcg ttgaccaagg ttttcagtta actagcctta aaagtgagct
    21001 ccagttgcgt atctttgtta cctttttctt tttaagttac tacggaagac gcacgttgcc
    21061 atttccaatc gaaaaaaaga agatcaaaga aattatggtc aagaataatt gtcagatgaa
    21121 tttgtttcag gaagaaagcc catatgagca atctttcttt attatgtatt ttgttgtgca
    21181 tctcatgaga gaatcccaag gaactccatt tgaatggaaa gctggagact cttatgtaca
    21241 gttgataaat atcgataaaa aagacttctt aatcttgaaa aatctcttag gaaaattgaa
    21301 gtacagcagc tatataaaag tagtgaacta ttttgtcaat acgatttttt cagaattttc
    21361 ggagaaagaa aaaaatgaaa tcagtgagca gatacgaaaa aatatgtcct actgttcgtt
    21421 ttcatccgct gattgcaaag aaaaaaacga agaatatgtt gttcaattat taacgcaaat
    21481 ttattttttc tcccgtattt tcccatacga tacgactcat ttgacgatcc gtgcaactta
    21541 ctttgcacag cagatgcaac accgccagat acgtctatat gctggatttc gtgcagaact
    21601 tcaattttat tccactctac tgaatcagaa tctgatttat tatttatcta gctatatctt
    21661 ttggatgctt atcggtaatc ctgaaattca ccattatact tctgataaaa aaatactgat
    21721 tatcagcgac ttaagtcttc gacatagtca atatattgaa gaatatatca gtgatattct
    21781 tgctgtgcat aaaattcact ctgagacaac tgctattact gaagatcagc tgtcgaaata
    21841 caatcttctc gagtatgatc tgatcgttac gaatcaaccc attttgaatg cccatgttcc
    21901 tcatatattg attgatgata gtgtgtcatt cgcaaatgaa gaagaactca tccgcttatt
    21961 cgagttatga ataatcaaaa aacagctatg tataaaccac cctgataaaa atttcaggac
    22021 aaatacacat agctgtttct ttttattgta ttccttcagc aatccaacga gatttatagc
    22081 gaaagtcttt taacaactct tcttttgtcc aattatttgc aatgagatcc gtcaaagtgt
    22141 tcattacgag ggtcatggaa aagtatagga aactaactgc ttcttccgac gattgaatgg
    22201 agaaatgatt gattcttaat aattccagcc atttttcgtt cattggagaa tcagccgata
    22261 gttctctacg tttggttgtc tgctgatcat ttcgctgggt taacaagtat aacctttgcc
    22321 aatagatact ttcgtgatct aagtcgcagc accactctaa atatttttcg atccccaaaa
    22381 agaaatgttg tttgttttcg ctaatgaatt ttaagatatc ttgatggact atgtgagaac
    22441 tgcttttgat cgtatacaaa taagcatctt ccaaatcttg aaaatattta taaaaagcac
    22501 cgcgcgacat ccccatcttg tcaacgattt ctgagacttt gacttggctg atgtgctgtg
    22561 agcaaaatat ctccagtaat aggtcatcga ttctctgttt ctttgcgttc gataaattga
    22621 aaaaagtttc ttttggcatc cacatctctc ctttatctct gattatacgt ggatagccct
    22681 tcattgtcat gaaggattca ttccttcttt ttctcttctt ctgtcaagta cccatcttcc
    22741 attcgataaa cgcgatcact ccattgaatc atacggacat catgagtaac cataatagtc
    22801 gctttttgtt tctcatgagc ttctttaact agtaatttga cgacttcata tgcatgatct
    22861 gtatccaaac tagctgttgg ttcgtctgct aagatcaagc ttggctcatt atataaagcg
    22921 cgagcaattg ccactctctg tctttctcca ccagaaaggt ctttaggata actattttta
    22981 agtgactgga tatctaaaga ttgcagcaag gaatcgattt ttgttgaatc ttcttttttt
    23041 gaagtaactt ggtcaactag ttcaaattgt tcctttaccg ttaaaaaagg aatcaaatta
    23101 gagctttgga gaataaatcc tatttcttta aagcgtaaat ctgctcgttt tttttctgga
    23161 agctcagtta gtgagtgacc gttgatcact atttctccgg aagtcggtgt ttgcaatcct
    23221 cctgaaatgg taaggaaagt acttttacct gaaccagaag gaccaatgat gctgacgaat
    23281 tctccttcct ttacataaaa atcaattgct ttcaaagctg agacctttgt atgaccagaa
    23341 ccaaatgttt tggtaacttt tttcatcgtt aatatttttt tcatttttat taacctccta
    23401 ttgctgtaat tgggtctact ttggttatcg tacgtatcga aaacaatccg cctagcattg
    23461 cgacaatgat caaaatccca ctatatagca accattgtga ccaaaaaaca gcaaaaggca
    23521 tcgcgcttgg taaaatcaga ctagtcaaat aagcaaacaa aagagcaagc agaacgccta
    23581 ccaccccaac aataaaggac tgtgctacta aagatttagc gatgaagaaa tttttgatcc
    23641 cttgtgcttt cattactcca aaaatagcag tcttctgaag tgtcatcacg tacatgaaaa
    23701 taccgaccac ggcagcaacc actaagaaca aaaagtatat catcgcattt aaggtcatat
    23761 tttgggcaga atagccagga atactttcaa tgaatgtccc aatagttaat aactgtagac
    23821 ccttcgatgc tttttctttc gaaattttag ctgaatcctt tattacgatc ccattgatcg
    23881 gctgttcttc ttcagaagca aacggctgtt gtccatactt caaagcagtc cacgtatcta
    23941 gggacgcata gatgacggga gaaactgtgt aataagtttc aggaaagata ccgacaatcg
    24001 tcaaaggatc gtcgtaactg ccaattttga tcgtatctcc aattttatag cctgaatcag
    24061 ccagattttg agagatgatg atttcattct ttgttttaaa ttcatgccct ttcgtgagtt
    24121 ttggtaataa aaatgcttct ttcgttgtgc cgaaaactgt aacattttct ttgttttttc
    24181 ctttgattgc tccgctgtac aatccgattg gtgctttctc tcctccttca atgtatttca
    24241 gatctcctct tgttaattgt gaagctgcaa atacttgatt tgcatcctcc gacaaaacaa
    24301 tctcttgtgc gtcccaatca tcgattgctt ttttaaactc ttctgaaagt ccattcgcta
    24361 gtcctgacaa catgaatacg acatacgcaa tcaagaacat aataccgacg atcaagccat
    24421 atcgtaattt agaatagcga atttctttga tagctagaaa catttgtttt tcctccctat
    24481 gatagattga tagtgacaaa atgtcacttg ttgaatagtg taactctaaa gtgacaattt
    24541 gtcacttgtg ttgttttttt cttatcaata aattgtctaa gacatctttc gataaaacct
    24601 taacatgagt cataaaaaat ttatttaagc cttatttatg ctttccatga taaactaaag
    24661 tgaccaaatc aatgaggtgg gaaaatgatt aaactattag tagtagaaga cgataaagta
    24721 ttatccaata atataaaaga aatactcgct gcaatcggcg agataacaca agtttatgat
    24781 ggtagtgaag gactgttcga gttggaaagt ggagtttatg atcttgcaat cttggatttg
    24841 atgatgccgg aaatgaatgg atatgaagtg ttagcaaaaa tacggaaaga aaaaatacag
    24901 actccagtat tgattttgac tgcaaaagat agtttggaag ataagattga aggatttgaa
    24961 cgaggtgcag atgattatct aacaaaacct ttctatcgag aagaactgat catgcgcgtg
    25021 aaagcattat tgaaacgctc gctaggtctc tttgatgaaa atgttctgaa gtataaggat
    25081 atcagttgtg atttaaaaag caatgaagtc ttgtataaag aacaagtttt gcccatacaa
    25141 ggaaaagagt ttgaattgct cgtttatttt ttacaaaata agggaatcat tttgacaaaa
    25201 gagcagatat ttgaccggat ctggggattt gattctgata cgacgattac agttattgaa
    25261 gtttatatga gcaacttacg aaaacatctg cgaagtactg gattggataa agaaatcaaa
    25321 accttacgta atgtcggtta tatactgcaa agggtgtgat gcaatgaatg gagatattac
    25381 acgaaagcaa cgtagtcgct tttttgcgac aaatgtagca gcatttgctt ttatcttttt
    25441 tcttttggga ttcattactt tacagatatt aaaccaatct gcatacagag aaacagatct
    25501 gtcattagag aaaataaatt ttgattctcg aacagtacaa ttagagatac aaagatatca
    25561 gcaaggcgat ccctttttaa acccgactca aacggaacaa aacagaacaa acatggaaca
    25621 gccattaatg gaaccatcaa atgaccgttt taacactcaa gtgatcttgt ggtctaaaaa
    25681 tggtgagata ttgaataaag cagctattgg tgggcgtttc aaccaaatca aagaattaaa
    25741 aatggatacg aaaaatctga ataccatcca aaatattgaa ttaaatgaag acagtggctc
    25801 atctgccctc tctttccgtt cgattacgaa aaaagcggag acgaatcaag atggcgttgc
    25861 ctacatccaa attttagaaa atacaaatca aatcaagaat tcattagcga cttttcgcac
    25921 gattatgatt ttgtgtatga ttgttttttg gattctctct attggtatca gttattactt
    25981 gtctagtcta agcatgcgcc ctattattct ttcttggaga agacaaaaag aatttgttga
    26041 gaatgcttct catgaattgc gcacaccgtt aacgattatt caaaatagtt tggaacatct
    26101 ttttaccaag cctaatcata ctattattga agaatccgaa agcatcgctc aagcattgtc
    26161 agagacgagg cgcctcactg gattgacttc ggatttgctg acaatcgctc ggaatgattc
    26221 tgatcagcag ctgctttcaa agcaaatgat caatacacaa gaatatatcg aacaacttgt
    26281 taaaccattt caagagatgg ccatcataga tggaaaagag tttattctcc ataacgatgc
    26341 aacattagaa gtaccaattg atgaaaaaaa aatccatcaa gttttggtta ttttgctgga
    26401 taatgcatta aaatatacga atccagaaga taagatcatt ttggattcat ctgttaacca
    26461 taaagaatgg ctgattcacg taaagaatac tggcccttct atcagtgaag aagatcaagc
    26521 aaggattttt gatcgatttt atcgggaaga tcaatctcgg gcgaaagaga ctggcggata
    26581 cgggcttggc ttggcaattg caaaacaggt aatcgaacag catcatggac agatttctgt
    26641 atccaattat cttccacaag gagtcgattt taaagtccaa ttgccgttga agcctaaaaa
    26701 ataatgctta tccctaaaaa agggctgtga caaaaatctt gtcacagccc ttttttgttg
    26761 cgaataaacg gtgttccaga agtagcttct tcggaaataa gccgaaattt ctttaagctg
    26821 accacttctg tcacaacctc tttagcttac gcttacgatt ttttattgcg gtcttcaatg
    26881 tgtacatcac cgcttgtgtc aatatccaat tcttctcggc taacatcttc tgtcactttt
    26941 tcaacatctt catgttcttc tttatgaatc ccaacttctt cacgaataac tgtatgtttg
    27001 cttacatcta cttgctcttc tttgattggg atagtaatcg tgtcatcttc caatttttcg
    27061 tctgttccct gtgaagattg atctgttact ggtttgcgtt cgatgacgat ttcttcacgt
    27121 ttgaccggta cttctaccgt ttcagtatcg ttgaccacat gtttgtggat gtcgacttct
    27181 ccagttgtca catcatgtgt atttacatct aatttttctt cttttagttg aattgtatca
    27241 tcatctagat ttggtttgcg atcgatgtct tcagtcttgc ttgtacccat tgtatcagtc
    27301 tcgccaggta cgtccatcgg atcagttcct ggtgtcatat tcaatggatc cgttgtattt
    27361 tgaaagcctg tcgtgccacc tacgtccatt ggatctgttc cagtgccagt ttctaatcca
    27421 gtccccattg tgcttgctgg gctaacagta tcttgtgtat ccattgtatc ttctgttgtt
    27481 tctggctgac gatagccttt tactgtgatg acaaattttc catcagaaat atctcctcta
    27541 tattgatata aaggatcatt ttctggtgta gccgtctcag aatcataggt atcaaaagaa
    27601 aaagtatctt ttattttttc ccataaagaa cggtcttctt caccatccat ccgattttca
    27661 tctgtttcaa catctatacc agacaatccg tcaaatccta gtctagatgc tgcttcatca
    27721 gtagtataaa taacgatatc atctcgttca tacccttcgt tacgcaaccg ttcaattact
    27781 gataatgctt cttccctcgt actatagctt ccgatgaccg ttaaattttt ctcttccatg
    27841 taaattcctc ctagcaaata gatttatgat tggtatattg ttact
//
LOCUS       DAZABD010000036        27686 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000036 DAZABD010000000
VERSION     DAZABD010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27686
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.036"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..924)
                     /locus_tag="ACHALG_001618"
     CDS             complement(<1..924)
                     /locus_tag="ACHALG_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN2587012.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLR"
     gene            complement(963..1697)
                     /locus_tag="ACHALG_001619"
     CDS             complement(963..1697)
                     /locus_tag="ACHALG_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN2587013.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIIEEFDNNILLLTSSIEEQLEEMIDEGIHSQVEDMNYPEEAVNEIIDN
                     INKLKQTISSIGKKYTKKKMKGGDTI"
     gene            1932..2207
                     /locus_tag="ACHALG_001620"
     CDS             1932..2207
                     /locus_tag="ACHALG_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN2587014.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMEQYKG"
     gene            2305..4302
                     /locus_tag="ACHALG_001621"
     CDS             2305..4302
                     /locus_tag="ACHALG_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587015.1"
                     /translation="METPMINIPINVDKERLLEKPVIEDEKKRIRKRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNKSEEFLETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKALSINLVHNSAFAIQKSIRYSENLEKFKIVFLLKEAITDYSKMIAVYEYLKKI
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHDDHNPSASIFTSKTGHSIFYCHSSNCGISENFIGVVMLILEKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMEDKLFRTINNYLEEMRRVYDALLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKWNKLLSFMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLKKVHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNFRSELSKEKQLILDEAHEVLQDLSWQGTYLMTEKELINTVC
                     ERLRNVGNNSVENALKENRGYFVKQGFTLERASKDIKKLYNVKASSSFLVYLAKGAGI
                     DEVIKELIASGVELVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(4378..4713)
                     /locus_tag="ACHALG_001622"
     CDS             complement(4378..4713)
                     /locus_tag="ACHALG_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN2587016.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            4839..5054
                     /locus_tag="ACHALG_001623"
     CDS             4839..5054
                     /locus_tag="ACHALG_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN2587017.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPINELFSDVFNKVNS"
     gene            5091..5453
                     /locus_tag="ACHALG_001624"
     CDS             5091..5453
                     /locus_tag="ACHALG_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFN2587018.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGADEVKKSTEQTFS
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            5499..5825
                     /locus_tag="ACHALG_001625"
     CDS             5499..5825
                     /locus_tag="ACHALG_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587019.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKATLDNGSAININVEYYPKKDRVYVEIPYT
                     EVLMLN"
     gene            6026..6802
                     /locus_tag="ACHALG_001626"
     CDS             6026..6802
                     /locus_tag="ACHALG_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587020.1"
                     /translation="MMKKNYLKTGIFLVIGTILVIQLSGCRNKDKDFYGYWSGTAEKI
                     PVDYNFKITKDRVIELQKNGEMVDFKYTLRHKKFDSTTFRSIMISKDELVKAIPELKE
                     TTGISNITFMPIDGEKDKLTVLVNLDEHLSTATLYRTTKDESPIGKVEKKNDTISRQT
                     VDKKIDTFIKTYAAIPPTWKLVNEDKGITINTDTEIPTDAQSLSDLLANLTGVSNAVS
                     EQVGKDIPLRLLENGKKDYSVVFKNGKVTDYNEIFEKVIE"
     gene            8558..9343
                     /locus_tag="ACHALG_001627"
     CDS             8558..9343
                     /locus_tag="ACHALG_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006701865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylase"
                     /protein_id="HFN2587021.1"
                     /translation="MVEDMVMVEEEQGRLIKSKKRVQKHGEVFTPQRTVKDMLDLPGI
                     KEACEELQTTFLEPSAGEGAFLVEVLTRKMAMVAREYNESLEQYENYSLLALSTLYGV
                     ELLEDNAQTCVMNMFQVFNDLYREQSKKHGTAMKRKVQDSAKTLISRNIKQGDFLTKR
                     TPDDNPIIFSEWTPKTLRKGQKKITVNRTEYSLEDIYSGVTREFGEAYSALPVFEQLD
                     LFSFDDEVDEEVSNDPILRYVAMPITEVYKEEMEEIDDDSVEI"
     gene            9321..13718
                     /locus_tag="ACHALG_001628"
     CDS             9321..13718
                     /locus_tag="ACHALG_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019788126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="HFN2587022.1"
                     /translation="MTTALKSKAKYFDYLPKYGSKLEHFLGIPNLVDAINTYRVTVNQ
                     IPNEVAIGTRRYSEVAARKIASNVGIDQQSMKYVDVLRELRVRNNITPTIADLFHKLR
                     KYGNVAAHENGIIPVSEAVESLVTLDTLLRVFARNLSGDKSLNPKEEITDEMFVITYN
                     TFDRKLIYIQSAKDADGTWKMYQGLEKIGDASVPENYEADFTPNSEYLREYASRRIKQ
                     YMTTAGVPFVLHWAQLAVNKKNQFFRDHDVHEVLKRSGYNPQTIGEDDTGKPNEWFSV
                     TVDVAKKAIQAVKDGKGSLDSEKDDFDEFKINFRPEQKDAIEQTKTVFKTKDEMLWNA
                     KMRFGKTLSSLQVIKESQYKKVLIMTHRPVVSDGWFTDFKKIFTDGSYIYGSKQDGEK
                     IQNLVDTEKPFIYFASIQDLRGSEWAGGKAGDKNYEFLAIDWDFIIIDEAHEGNETEL
                     ANNVKAKLRKEHTKVLELSGTPFNLFDKYDEDNIFTWDYTMEQEAKEKWSVEHPNEPN
                     PYEGLPKVSMFTFEIPNKFDYFDENKAFNFREFFRVKEDDETKLLHEGDVRKFLDYIT
                     TNDEKTNFPFSKPEFRENLRHTLWLMPGVKEANAFERVLETHPIFKDYNIANVVKTGD
                     SEHASDSDLELVRKAIGNPAKSKTITLTVRKLTTGVNVPEWTGVFFLSNTESPTSYLQ
                     AAFRAQTPFNHAELGIKKNCYIFDFAPDRALKIMSGSVGLTSKKGKVNSSEQKKKLER
                     MLNFLPILGKQGNVMKEFSVDRMLTALKKAYAEKAVRTGFEDSSLYNDNLLNLDQADL
                     TKFEDLKKIVGSSKPTKVKDFIVSDNGMSEEEYEKASRAEKKPKRQRTKEEQEAIDRA
                     KRLREQRNKMISILRGVSIRIPMMIYGMDIDIKDDVTIEMFLNLVDEASWNEFMPEGL
                     SKGRFREFIEYYDADVFVEAGRIIRRRAKSYDELDVIERTRKIGELFSTFKNPDKETV
                     LTPWRVVNMHLGNTIGGLSFFDEEYQNSTENGQPIVRWRKTDCTDDIFKENRKILEIN
                     SKTGLYPLYAATSMYYVQLLKQSDINAGRVDSEAIWEKVLSEQIFVISKTPMAKTITK
                     RTLIGYNAWKVNVEYSSELVTSMKRSINDGLTNLKGVYKNVKFDVIIGNPPYQEKDGG
                     SGASATPLYNLFVETSVKLSERYVSMITPSKWFAGGKGLDKYRDFMLNSSSLKVMVDI
                     VNGKEIFPNTSIGGGVSYFLWDKKYNGLTQYTNQHDGVSNTQERKMNEFPVFVRYNEA
                     ISAIHKIGEHASVGDFVISRNPFGLPTKIRGEEVQDSRNSIKVISSGGVGFVPLEDIT
                     KGQDMVGKYKVISSSLTSEHAGEPNKNGMFKVISTNRVIKPNEVVTDSYLVLFSADTE
                     TEAINYLKYLRTRFYRFLLLQAVTSIHLSKDKFMFIPVQDFTSGSDINWNVGIEEVDM
                     QLFKKYSFDASEILFIDQTIVPILHEEYY"
     gene            complement(15158..15823)
                     /locus_tag="ACHALG_001629"
     CDS             complement(15158..15823)
                     /locus_tag="ACHALG_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN2587023.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDF
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            15949..17985
                     /locus_tag="ACHALG_001630"
     CDS             15949..17985
                     /locus_tag="ACHALG_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HFN2587024.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERVISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            17989..18627
                     /locus_tag="ACHALG_001631"
     CDS             17989..18627
                     /locus_tag="ACHALG_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN2587025.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            18655..19083
                     /locus_tag="ACHALG_001632"
     CDS             18655..19083
                     /locus_tag="ACHALG_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587026.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            19540..20451
                     /locus_tag="ACHALG_001633"
     CDS             19540..20451
                     /locus_tag="ACHALG_001633"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFN2587027.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            20668..21105
                     /locus_tag="ACHALG_001634"
     CDS             20668..21105
                     /locus_tag="ACHALG_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587028.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            21200..22084
                     /locus_tag="ACHALG_001635"
     CDS             21200..22084
                     /locus_tag="ACHALG_001635"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFN2587029.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            22308..25475
                     /locus_tag="ACHALG_001636"
     CDS             22308..25475
                     /locus_tag="ACHALG_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFN2587030.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            25543..26040
                     /locus_tag="ACHALG_001637"
     CDS             25543..26040
                     /locus_tag="ACHALG_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HFN2587031.1"
                     /translation="MILLLGFILWQTPVHASEFNFAVDFELPANQIDKNKTYINVRME
                     PGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESKSKFDETLSYDMKNLAK
                     TVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKENVSNKSTVGVVNQFSQV
                     KAILI"
     gene            26059..26523
                     /locus_tag="ACHALG_001638"
                     /pseudo
     CDS             26059..26523
                     /locus_tag="ACHALG_001638"
                     /inference="COORDINATES: protein motif:HMM:NF023225.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
     gene            26520..27278
                     /locus_tag="ACHALG_001639"
     CDS             26520..27278
                     /locus_tag="ACHALG_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFN2587032.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(27364..27678)
                     /locus_tag="ACHALG_001640"
                     /pseudo
     CDS             complement(27364..27678)
                     /locus_tag="ACHALG_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
BASE COUNT         9449 a         4285 c         5574 g         8378 t
ORIGIN      
        1 tcttaaccaa tcattaacag tgtttggaat gtatagagtg tttttaactg ttgtaaaaac
       61 atgttgctct ttagagctag tattgtatcc aagttttaaa tactcttctt tttgctgtaa
      121 tttccagttg ttgaggatag aaagagtctc agtatctaaa gagataactc gtctagaagc
      181 tcttgttttc ggagattgaa ttataacttt accaaattca tctacagcaa gagttttatt
      241 gacatgtagt tctttattaa acgtgtctat gtctttccac tgtaaagcga gtacttcact
      301 ctttctcata cctgtgtatg ccaataatct gaagaaggcg tattgtttca tgttaccaaa
      361 atctttaaaa gcatccagta aattattaag ctcctctttt gagtaaaatt tacgattttt
      421 ctcatactct ttttttcgtg gaagaagtgt tttttccata ggattattgt caatatattg
      481 cattgcaacc ccgtagcgta aaatttgtgc agtctgtttt ctcatataat cgtaactttc
      541 atatttactg tgccattcat ctaccacttt ttgacaatat gggacagaga tattcgatag
      601 tttaagctta ccaaatgctg gaagtatttg attatttgcg tatcttactg agattgcgat
      661 tgttgaagct ttaacactgg tacgctgatg agttatccat tcattataca ggtcttcaaa
      721 tgtcatatca ggtgctttct ttttaacacc gtataatatt tgatgtctgt actcgtcata
      781 atctttttga gcttcttgtt tgctactaaa acctcttctc gtagtattta ttttttcacc
      841 agttcgtgga tcggtggcaa tgtgtccttt atacatccac gccacttgac cgtttttctt
      901 tttgtacttt gatatttttg ccatgaaatc atctcctctt agtttacaag gagtacttac
      961 cactagatgg tatcacctcc cttcattttt ttcttagtgt attttttccc aattgatgaa
     1021 atcgtttgtt ttagcttgtt tatattatca ataatttcgt ttacagcttc ttcgggataa
     1081 ttcatgtctt ccacttgact atgtatccct tcatctatca tttcttctag ttgttcctct
     1141 atactgctag tgagcagaag aatgttattg tcaaattctt ctataatagc attgatgaat
     1201 aattcttcta tttttaagat attgaaaaga ctaatttttt catttttaac tttattacaa
     1261 gtcttgttag caataaattc ttgcatctct ggttctaatt tggaatatct atccagccat
     1321 gtatctaaat tagagtgtac tggtgggaac tgttgacgaa gatgctggaa caggttttta
     1381 tctggaaatg ttataccatc tagtacaatg ctatctctat aaagaataat tcccaaaata
     1441 tattcttcta cattgccaca aactaactcc tctatactca tatttcccaa atcagcaatt
     1501 cgggataatc gctctggtat tggtaaatag gttccctttt cccattcgta gatagttttt
     1561 ttgtcaatca attgaccatt gttaatcata agaccaaatg ctggttgtgt taatttttta
     1621 ctctttctta tagcttttat ttttgttccg agtatctttt ttcgttctaa ataatttatt
     1681 ggtggacgct ttatcatatt gaccacacct cctaaaatta gtatacctca aaaagggggt
     1741 gttgtaaata aattcacaca acatattgac accccttttg tatcgaggta ttatgtgtat
     1801 gtcgatgaac tgataatcat tttttatttg attaatcggt taataacaac aaaatacttt
     1861 tattttgatc aaataatacg aagatgattc ttagaaaatt tgttcaagta atcacgaaag
     1921 gagggggaaa tatgtctcta caaatcattg atagcattgt ttctatacaa gttgaacaac
     1981 aagtgaaaga gataattaca gtgctaaagc ctatgctaga agacattatt tctggtgtta
     2041 ctctagagta tatggactat cagcaggcaa gtgaatatct aggtgtatca gtaggaacga
     2101 ttagaaaata tgtttctcaa tacgggttac ctgttataaa aattgatacg gtcattagat
     2161 tcaaaaaatc tgatattgat agtttcatgg aacagtataa aggttgagta aacaaataag
     2221 actaaaacat atagtaataa agagtttgtg tttattatct gtatgtttta gtctttaaca
     2281 gaattttata aggaggaaac acaaatggaa acaccaatga ttaatattcc aattaatgta
     2341 gataaagaaa gactattaga gaaaccagtt atagaggatg aaaaaaagag aatacgaaaa
     2401 agatgtttag ataactacag agaagttaca agagatgagt ttgtagagat actaaattca
     2461 tcacaatcat ttattccaag taagacaaag aataaaggca acaaaagtga agagttttta
     2521 gaaactagag taattattct ggatgtcgat aacactgtta aagatgagaa ggggaaggta
     2581 gtagatttaa gtaaagatga tagtagatac ttaagcatag aaaaggcttt atctatcaat
     2641 ttagttcata attctgcgtt tgcgatacaa aaaagtattc ggtactctga aaatctagaa
     2701 aaattcaaga ttgtctttct attaaaagaa gcaattactg actatagtaa aatgattgct
     2761 gtttacgaat acttgaaaaa aatcataccc gggtgtgatg acaatgtaaa tgcttcgaat
     2821 agaatgtttt ttggaggata taagtcagat gcagtgataa taattaatga agacaacatg
     2881 ttagatatta ctgagcttcc gattgatttt accatgacat gttctaaaaa ttgtcaaagt
     2941 gattcaggga gagagtatga actagtcaat gaaactgatt ttgttaagtt gataaaaaat
     3001 gacgacaaag aagaaatgaa acagtggttt aaagactgcc tgtttgattc ctccgacatg
     3061 tcttttaatg agatttatga aagattgtta accattgata tgaataaatt acttaagtca
     3121 agtaaaaact taagatgtct gtttcacgac gaccataatc catctgctag tatttttacc
     3181 agtaaaactg ggcattccat tttttattgt cattccagta attgtggtat cagtgaaaat
     3241 tttattgggg ttgtgatgtt aattctagag aaagatagtc gggtagaaac atttaattgg
     3301 ctattagaga atctagacct gtatcctaca cattttcgaa aattgaaaga ggaatcaaaa
     3361 gtactttttg acactcttga atctatggaa gacaagcttt tcaggacaat aaataattat
     3421 ctagaggaaa tgcgacgtgt ctatgatgcc ttgctttctg atgttaattt ttttgatgat
     3481 agtaaggaaa gtgtgacttg tattttatca ggagaacaac tagctaaaaa aatgagttct
     3541 tactatggta aaacttatga catagataaa tggaataagt tactatcatt tatgacattt
     3601 ataggattga taaaaaaatt agatgatgaa gatgttcctt tgaagatgct cgaataccta
     3661 aataggttaa aggaagagaa agcgttagga aactttaatc ataagagaag caatgtctat
     3721 cgattagacg tttttaatgc aacttctgtg attcaaaaat tgaatgaaga tatccttcca
     3781 cttctgaaaa aagttcattt tacatatcaa tatttttctt atgaatgggt taagatatgt
     3841 tttaacgaaa aagaggctaa aagagtattt cctcaaaatt ttcggtcaga actcagcaag
     3901 gaaaaacaat tgattttaga tgaggcacat gaagttttac aggacttatc gtggcaaggg
     3961 acatatctaa tgacagagaa agaacttatt aacactgttt gtgaacgtct acgtaatgtt
     4021 gggaataaca gcgtggaaaa tgctttaaaa gaaaacagag gctactttgt taaacaagga
     4081 ttcactcttg aacgagcttc taaggatatt aagaaactat acaatgtgaa agcaagcagt
     4141 agttttttag tctacctagc aaaaggagca gggatagatg aagtaataaa agaattgata
     4201 gcatctggtg ttgaattagt tcctctttat caatttacaa gtgtaacaag agatgatgga
     4261 cggagagtaa ggaagagaaa agacccaatt gtattaaact agacaggtta tccatagctc
     4321 ttaatcttat ttcatctcaa tacacccacc gttaaagttc ggtgggttat ttatttttca
     4381 atctttcaat agctgattgc gttcattttc ttgccttttt aatagcttca tatagtcaat
     4441 tgttcgaact gcatcttcag tgctaagaga acgatagaga gccaaaaact cttcctcata
     4501 atctacgaca acattgtcta gaaaatggct tacaggggtt cctagggctc tagaaagagc
     4561 tgttaagcta tcgaggttag gctctgttac agaattttcc catcttgcta ctgcctgttg
     4621 tgtagagaag attttttcag ctaattcttt ttgtgtccac ccgagagctt ttcgcttttc
     4681 tctaatgacc tttgcatatt gtttctgttt cattaataca cccccatctc agtatagtac
     4741 aacaagttgt tgtatttgta atggtatttg aagtttttat attttatgag ttgaaataca
     4801 tcatattgtt gtatataata aaacaggagg ggagaatcat gaaaaaagta agtaatctca
     4861 gccgatacag aaaagaaaaa ggactttctc aaacggagtt agcaaaaaaa atgaatgtta
     4921 cacagcaatg tgtaagttcg tggcaaacag ggagaacgat tcctaaaccc tatcaaatga
     4981 aaatgctttc tgagatatta agtgtaccaa taaacgagct gttttctgat gtgtttaata
     5041 aagtaaacag ttaactatta agcaattgaa tgattttagg aggagaagac atgtattgta
     5101 gaaaatgtgg taaagaaata acagatgaaa gtaagtattg ccagttttgc ggagcattac
     5161 aagaaacaga tgagacaact ggagcagacg aagtaaagaa aagtactgag cagacattta
     5221 gtgaaatgaa ggaacaaatc gtacatggac aagagacagg gaagctgtat ttgattattg
     5281 gttggatttc tatggtagta tcgttagcat ttattcctgt tgtatttggt gctgttgctg
     5341 ttattatggg ctacttatat cgagaaaaag atgagaaaat gggtacgatt ttgatgatag
     5401 caggggtagc tggagcaatc tttggtgtac ttttagggat gtcaatgtac tgataagagg
     5461 gataagagca aattattttg gagataggag ataaaaagtt gaataaagta aatatgaaca
     5521 agaagtttgt cattataggt atcgttgtct tggctattat aggtatcatt ttttcaaaaa
     5581 tgacaggtac atctggatta gagaagtcat ctaaaccatt ggtgacacag attttacaag
     5641 aacagtacaa cttaaatagg tcatgtgatg atgtgacgat tactcatgaa aatggagata
     5701 acacataccg ggctaaagct acgttagaca atggaagtgc tatcaatata aatgtcgagt
     5761 actatccaaa gaaagaccgt gtatatgtag agataccata tactgaggtg ttaatgttaa
     5821 attgatttaa atgtattatg tataccgtcc aaacaggaac tcctaaaggt gtaagcttac
     5881 taaggagttt ttttgattcc ctgtaaataa aatactaata tagatgagaa aaataatgtt
     5941 ataataattg atggaaattg tttttaaaga gctttaaaaa ttgggttctt acattgttga
     6001 ttaaatgctt taaaagagga gctttatgat gaaaaagaat tatttaaaaa caggtatatt
     6061 tttggttata ggaactatac tggttattca attatcaggt tgtagaaata aagataaaga
     6121 tttctatgga tattggtcag gaactgctga gaaaattcct gtagactata attttaaaat
     6181 aactaaagac agagtaatag aactacaaaa aaatggtgaa atggtggatt ttaagtatac
     6241 tctaagacat aaaaaatttg attcaactac atttcgttca atcatgatat caaaagatga
     6301 attagttaaa gcgattccag aattaaagga aaccacggga ataagcaata taacgttcat
     6361 gcctattgac ggtgaaaaag ataaattaac tgtgttggta aatttggatg aacacttatc
     6421 aacagccact ctttatagga ctacgaaaga tgagagtcca attggtaagg tagaaaagaa
     6481 aaacgataca atatcaagac aaactgtaga taaaaaaatt gatacgttca taaaaacata
     6541 cgcggctata ccacctactt ggaaacttgt aaatgaagat aagggaataa cgataaatac
     6601 tgatacagag attccgactg atgctcaatc tttatcagat ttattagcta atctaacagg
     6661 agtcagtaat gcggtgtcag agcaagtcgg taaagatatt ccacttaggt tacttgaaaa
     6721 tggaaaaaaa gattattctg tggtatttaa gaacggaaag gtaactgatt acaacgaaat
     6781 atttgagaaa gtcattgaat aatggttttt ctatcttttt attaactacc ctaccccgtt
     6841 gttttttgac cgtgtttcga gaggaatcat tagaaagcat aggctaacta ggggtttctt
     6901 tttcttttat tttttgggaa tccgcttaag tgaaatgtat ctgaaatgaa acggagataa
     6961 agtgattttt tgctgtaatg acaaggtttt agctgtttca gataccatgt aacccaaaat
     7021 agtttattga gaaaatagga gagtatttga tactaactag tagggaaaaa tgagataaaa
     7081 agtaaatgga atcatttcag gtaatctgaa ataaaaaaat atacagtcct gtggcacttt
     7141 acctatggga ctgtatattt tttactctca gcgttttttg actccgtgtc tgactccgtt
     7201 ttattgattt tatatgttat tcagtgaccc tttaacaggt caatttttat gtttttgtct
     7261 tcgtatgact cagtttgata cctagtgatt tccatggtaa tacgggatgt tgaacaagct
     7321 agagaagttc tgcaggggtt aattgcaaac aattcagata aacaaattac aatagattat
     7381 ttcaagaggg aaattaaaaa atcagaggaa aaatatttag atttaaagtc ggatatccta
     7441 ttagcagacg atataggttc actaactgtc gaagaagata gcctattcta cgtctattgt
     7501 gcattgactt cgtaaaagaa gacgtctttt ccaggtatac agttctttta tctacttcat
     7561 atgaaaattt gggtacaaac gacaaggaag tcagttttct ggtcagacta tcgtacgaac
     7621 atcttgttgt ggtatttaca ctcagctttg atgatgagta caagagtttt tcagcgaaca
     7681 agacagaaca tcgaaagagg tttccagacg aagttttaca ggctgggctg aagtattatc
     7741 aagaaggact caccattaag aagtctttat cgagaatata agaagataca ggtatagaag
     7801 tcagtcggtc aacattctgt gattacctaa acagcaagga gtaacaggtg aacgggaaaa
     7861 agagcacagg gagggctatg ttcactggct gaagaagcaa gataaacctg tttatgactt
     7921 gttctttgaa ataaagaaag ctttagatag tcagtcatta gagaacttca ttgatgggta
     7981 tgatgaaaag ttatctaatg agcaaatgat tgcgataatg aaagaggtgt ttcgtcagta
     8041 tgggttatca aataaacgta ggaaagaggt ttttgaccac gttaagacaa taatgagctt
     8101 ttaccacacg accctcggct aaatctaaag agggcgaaaa agggacgagt ggtaaaattt
     8161 aatgggaata gctaaaagta aataataata agtcaaagac tgcctagttt ggtggtcttt
     8221 tttcatgttc ataaaaataa aataggacag acgaacatct tcgctaactc ccctgtgttg
     8281 caagaggcaa actctataat tctccagtta agctatattt tgttctttat tttgagatag
     8341 aggaaaaaac aaaaatgtgt taaaattgaa cataagcata attagaaatt gtcgagtttt
     8401 ttcaagtaaa aatcttgact tgtaaggagg aaaagggcat gatgacacgc aatcacagta
     8461 ctagccagca gtctgcccaa aagcccatat atgaaagtga taaccgattc gtagcatccg
     8521 tttcggttat ttgtgttggg aagatgggtg atttttagtg gttgaagata tggtgatggt
     8581 tgaagaggaa caaggacgac taatcaaaag taaaaaacgt gtccagaagc acggtgaggt
     8641 atttacaccg caaagaactg tcaaagatat gttggatttg ccaggcataa aagaggcttg
     8701 cgaggagctg cagacgactt tcttagagcc ttcagctgga gaaggtgctt tcttagttga
     8761 ggttttgact agaaaaatgg caatggttgc tcgtgaatat aatgagtccc tagaacagta
     8821 tgaaaattac tccttactgg cgctttcaac tctatatggc gtggagttac tcgaagacaa
     8881 tgctcagacc tgtgtaatga acatgtttca agtattcaac gatttgtaca gagaacaatc
     8941 gaaaaaacat ggtaccgcaa tgaaaaggaa agtccaagac agtgcaaaga ctttgatttc
     9001 taggaatatc aaacagggag attttcttac aaaacgaaca ccagatgata atccgattat
     9061 ttttagtgag tggacaccta agacgctaag aaaaggacag aaaaaaatca ctgtaaatcg
     9121 aactgaatat tctttggagg atatttattc aggggtaaca cgagagtttg gagaggcata
     9181 ctcagctctg cctgtatttg aacagctcga tttgttttca tttgatgacg aggtcgatga
     9241 agaagtttca aatgatccaa tattacgtta tgtagcgatg ccgataactg aggtttataa
     9301 ggaagagatg gaggaaattg atgacgacag cgttgaaatc taaagctaaa tacttcgatt
     9361 acttacctaa atatggttct aagctagaac acttccttgg gattcctaat ttagtagatg
     9421 caattaacac gtaccgtgta accgttaatc aaatacctaa tgaagttgct attggaacta
     9481 gacgttattc ggaggtagca gcacggaaga ttgcttcaaa tgtgggtatt gaccagcaaa
     9541 gtatgaagta tgttgatgta cttcgcgagc ttagggtcag aaataatatt actcctacga
     9601 tagctgactt atttcataag ttgagaaaat atggaaatgt tgcagcacat gaaaatggaa
     9661 ttatacctgt ttcagaggcg gtcgaatctt tagttactct tgataccttg ctaagagtct
     9721 ttgcacgcaa tctgtctggg gataaatcgt taaacccaaa ggaagaaatt acggatgaga
     9781 tgtttgttat cacctataac acctttgacc gtaaacttat ttacatccag agtgccaagg
     9841 atgctgacgg cacatggaaa atgtaccaag gactggaaaa gattggggat gcatcagtac
     9901 cagagaatta tgaggcagac tttactccta atagcgagta tttaagagag tatgcaagta
     9961 gaagaataaa gcagtacatg acaacagcgg gggtaccttt tgtccttcat tgggcgcaat
    10021 tggctgttaa taagaagaac caatttttcc gtgaccatga cgtgcatgaa gtattaaagc
    10081 gttcgggcta taatcctcaa acgattggcg aagatgacac agggaagcct aatgagtggt
    10141 tcagtgtaac agttgacgtt gccaagaaag caatccaagc ggttaaagat ggtaaaggta
    10201 gtcttgactc agaaaaagat gactttgacg agttcaaaat taattttaga ccagaacaaa
    10261 aagatgctat tgagcagact aagactgtat tcaaaacgaa agatgagatg ctttggaatg
    10321 ccaagatgcg ttttggtaag acgctttctt ctcttcaagt gattaaagag agccaataca
    10381 aaaaagttct tatcatgact catcgacctg ttgtaagtga tggttggttt acggatttca
    10441 aaaaaatctt cacagatggt tcgtacatct atggttcaaa acaagatgga gaaaagattc
    10501 aaaaccttgt tgataccgaa aaacctttca tctattttgc aagtattcag gacttacgcg
    10561 gttctgagtg ggcaggtgga aaagcagggg ataaaaacta tgaattctta gcgattgact
    10621 gggattttat tattattgat gaagctcacg aaggaaacga aacggaatta gctaataacg
    10681 taaaggctaa gttgcggaaa gagcatacca aggtcttaga gctttcgggt acaccattta
    10741 accttttcga taagtatgat gaggataata ttttcacttg ggattatact atggagcaag
    10801 aagctaagga gaaatggtca gtagagcatc ccaatgaacc taacccatac gaaggtttac
    10861 cgaaagtatc tatgttcacc tttgaaatac caaataaatt tgattatttc gatgaaaata
    10921 aggcatttaa cttccgagaa ttctttaggg ttaaggaaga tgatgaaaca aagttgcttc
    10981 atgaaggtga cgtacgcaag tttcttgatt atattactac aaacgatgaa aagactaatt
    11041 ttcctttctc aaaaccagag tttagagaaa acttacggca tactttatgg ctgatgccgg
    11101 gggttaaaga ggcaaatgct tttgaaagag tgctcgaaac gcaccctata ttcaaagatt
    11161 acaatattgc aaacgtagtc aagacaggtg acagtgagca tgctagtgat tcagatttag
    11221 aattggtgcg taaagctatt ggtaatccag ctaagtctaa gacgataacc ttaacagttc
    11281 gcaagcttac aacaggagtt aatgttccag agtggacagg ggtatttttc ctttcaaaca
    11341 cagaaagtcc tacatcttat ctgcaggcag cgttccgtgc acagacacct tttaaccatg
    11401 cagagcttgg aattaagaaa aactgctaca tctttgattt tgcacctgac cgtgctttaa
    11461 aaattatgag tggaagtgtc ggactaacta gcaagaaggg aaaggttaat agttcagagc
    11521 aaaagaaaaa actagagcgc atgctaaatt tcttacctat ccttggtaaa caggggaatg
    11581 tgatgaaaga atttagcgtt gaccgaatgc ttacggcact gaagaaggct tatgcagaaa
    11641 aagctgtacg gactggcttt gaagacagct ctctttacaa tgacaaccta ttaaatttag
    11701 accaagctga tcttaccaaa tttgaagacc ttaaaaaaat tgttggctca agtaaaccaa
    11761 ccaaagtgaa agacttcatt gtttcagata acggtatgag tgaagaggag tatgaaaaag
    11821 cttcgagggc agaaaagaaa ccaaaacgtc aacgtaccaa agaggaacag gaagcaatag
    11881 atagggcaaa gaggcttcga gagcagcgaa ataaaatgat ttccattttg aggggcgttt
    11941 ctattcgcat acctatgatg atttatggga tggacattga tattaaagat gacgttacta
    12001 ttgagatgtt tcttaatttg gttgatgagg cttcatggaa tgagttcatg ccagaggggt
    12061 taagtaaagg gcgattcaga gagtttattg agtactatga tgcagacgtt tttgtggagg
    12121 caggacgcat aatccgtaga cgtgcaaagt cttatgatga gttagatgtt attgaacgta
    12181 ctaggaaaat aggtgaatta tttagcactt ttaaaaatcc agacaaggaa accgtattga
    12241 ctccatggcg agttgttaat atgcaccttg gtaacactat cggtggactt tccttctttg
    12301 atgaagaata ccaaaattct acggagaacg gtcaaccaat cgtgcgatgg aggaaaactg
    12361 actgtacaga tgatattttt aaggaaaata gaaagattct tgagataaac tcaaaaacag
    12421 ggctatatcc tttatatgca gcaacgagta tgtactatgt tcagctcctt aaacaaagcg
    12481 atattaatgc tggaagggta gattcagagg caatatggga aaaagtatta tcagagcaaa
    12541 tttttgtcat ttctaaaact ccaatggcta agacgataac gaagaggact ttgattggtt
    12601 ataacgcatg gaaggtaaat gttgaatact caagtgaatt agttacgagt atgaaacgtt
    12661 caatcaatga cggtttaacg aatttgaaag gagtttataa aaacgtgaaa tttgatgtga
    12721 taattggtaa cccaccttat caggagaaag atggaggttc aggagctagt gctacaccgc
    12781 tatataatct atttgtagaa acctctgtta agttatcaga acgctatgtt tcgatgatta
    12841 ctccttcgaa gtggtttgca ggtggtaaag ggttagataa gtaccgtgac tttatgctta
    12901 acagctctag cttgaaggtg atggtggata tagtaaatgg gaaagaaata ttcccaaaca
    12961 ccagtattgg tggcggtgtt agctatttct tgtgggataa gaaatataat ggattgacac
    13021 agtatactaa tcaacatgat ggtgtatcaa atacacaaga gcgaaaaatg aacgagtttc
    13081 cagtgtttgt gcgatataat gaagccattt ctgcaattca caaaattggg gaacacgcta
    13141 gtgttggtga ctttgttata tcacgtaacc catttgggct tccaacgaaa attcgagggg
    13201 aagaagtgca ggattccaga aattcaataa aagtaatctc tagtggtggg gtaggctttg
    13261 ttccactgga agatatcact aaaggacagg atatggttgg aaagtataaa gtcattagta
    13321 gctcactgac tagcgaacat gcaggtgaac cgaacaaaaa tggtatgttt aaggtgattt
    13381 ctactaacag agttattaaa cctaatgaag tagttacaga ttcataccta gtgctatttt
    13441 ctgctgatac cgaaaccgaa gcaattaatt atctgaagta tttacgaaca cgtttttaca
    13501 gatttttatt acttcaagca gtgacatcta ttcacctatc caaagataag ttcatgttca
    13561 taccagtaca agattttacc tcaggttcag acattaactg gaatgtaggt attgaagaag
    13621 ttgatatgca gttgttcaag aaatattctt tcgatgcaag tgagatattg tttattgacc
    13681 aaacaatcgt cccgattctt cacgaggaat attactaaga gaacaagcta acaaatgagg
    13741 cttagaaccc tgttttcttg attaattgcc ccactttaaa tcaagattta actgtctatt
    13801 atcaacaggt caaggtaaaa taaaggatgt tagcaagtaa agagattgat ttgagtctac
    13861 atcgaacaag ggaataatcc aataaaaaat gatttagaat atcaaaataa caaaaaagag
    13921 gactacctgt attagtcctc ttttttgacc agattttgac cagtctattt tacattctaa
    13981 tatattcata tgagctaatt ttatgattaa atggtaagtt tttccttgtt tgttttagtt
    14041 taaactagtg tgtaattcaa aaatctatat ggaagggtct gtggggtaat acttaaataa
    14101 aggctaggaa cctgataaca tcaaggttcc tagccttttt ataataaaat gaagcgaaaa
    14161 taatttcttt taaagagccg agattatcag ctatataaga gtgaatttga tgcaaaaaac
    14221 agagccgaaa cagtcttggt gttcgtagct ctgcttatat gtttcgaatt taagacaaag
    14281 agttggcaaa agatgttgat aaggaagata agataactgt gttatataaa ctaggtaact
    14341 aactgtaaca agctaatttt aagagattct tattagaaga cgtttaggaa tataactaag
    14401 aaaatgatta tacaggtaat tgtcatttca ataatgtttt ctaattttcg cgtctttgtt
    14461 ttaagaaaaa ggccagaatc agttggaaaa aagttcgtac gatagtgaag aggggcaaaa
    14521 tttcctacta atattcccac taacaagaga taaaacggcc aggttggtgt gattcctcta
    14581 taaaaaaatg cccatgctac cgaaatccca tagattaaca actgaacaat ttttacaatg
    14641 aaaaaattgt tctgccatta ctcaaagtct cttcctcctt gtatcgttta tttgtttcat
    14701 tttatcatta tgataggcgc ttgaacaatc cattttgtat tttattgagt actcttggat
    14761 aaactaccag cgattatcat atttatttat gttactgtaa gccatcatat aaaagtaaat
    14821 gagcagatca tagaaatata aaaaagaact aaaattgatc taaattatgt gcagcttgac
    14881 tacgtaccaa tctatattac ttactagatt ggtcgttaat ctattcggat cgaatgagca
    14941 aaatattttt gtgaatgtcc atccttgatt atgattccaa aaagaatcat tgggaggctg
    15001 tggcgtaaat attatactat aagccagaaa tacctatcat tctgaacttt tttatttagg
    15061 aactttcatc tcaaggcttt ttcaattaat ttgtacccct gttttttgga taagatcatg
    15121 tgcattgatt ttttctatct ctacgttcat ctcctactta atgcctatct ggctcctgag
    15181 ggcttctttt taaggcgtcg tcatagtctt ggctgaggat ttcttggtaa tctgagggaa
    15241 tggcttccca agtgagcagg cgaacgttca tgacgtgttt tcctttgtgt ttgattacag
    15301 catggttttt ctctttttct acctctccag cgtgaaaaga cagccatgta ccatggccaa
    15361 agatatcatt acatacccaa cgttcgatgg aattgtccac gtatcctaca gggagtgtgg
    15421 tatagccgat ttgtggtttc aaaaaaggat tgaatcgatc gatcgtttca tttttgtatt
    15481 gataattgat aagaaaaaga tagaccaaac aaaagacgag cagaaagaaa gatagtgtct
    15541 ttttgagtga gaagggttgt tttgatgaat aagatttctt gttaacaaga ggttcatctg
    15601 gcaatgttgg ttctattgat tcggtagctg attcaataga aggaaaatca atcggcagac
    15661 ctaaagtatg atgtaaaagt gacagcattt caaagtctgg cattgtacga gaattttccc
    15721 aactagaaat cgttttatcg gaaacaccaa ttttttcgcc taattctttt tgcgtaagct
    15781 tacaatttct gcggtaagtt cgtatttttt ctccggtgtt catcttcttc tcctttcgat
    15841 tctacagtat atagatttat tctacatgac ctatacactt gatagatacg tttttttgat
    15901 gaaaaacggt atgattttat caaagaatca tttaagtaag ggagagtggt gataatcaaa
    15961 aaaaagttca cagttttact cacaatcata gtgaccggta tcatgagtgg ttttgccttg
    16021 gttagtataa ataaacaggt actctatcgg acaaatgaat ttttccatca aacagatact
    16081 tatactccga ttcgattgcc acaacatttt attgggcaag aagaatatga gcagctaatc
    16141 atggtatttg aaaatgtctc atcagaaact gagctttctt atatgaaaaa aacagtaaat
    16201 gaatccaatg aagtagatac atcttggaac ttcaagatac agccaaagct tgaaatgaca
    16261 ttgtattatc aaggggatgt caaatcttct cacgagaagg taccctattc taatacctcc
    16321 tttcgcgtaa cgtccattga agaatcgatc aaaataaaag attttgaagg tgaatttttc
    16381 ttgatgacga gagaaaaaga agcctatgaa cgggtcattt cactattcac tgatcgattc
    16441 aatcaagcat ttcacactga ttatgccact aatatttttg aagactttga aaatagctat
    16501 tcgtattcgg ttatttcaaa tacagacgta tataattatc gagtgtattt aactattgga
    16561 ttgattcttt tattcctttt gttatcatcc tttttcttat tgatgaataa agaaatcagt
    16621 ttattgttga ataatgactt ttcattacat caagtattgt ggtgtttatt ggggaaaaag
    16681 aaaatcatta ttacattcat aattgcagga atcattggtg gttttatttt tatgtattat
    16741 aaggagcaat ttctttattt gttccagatg tacgttttct tgttcagcgg aatatatggt
    16801 atctctttga tttctctttt attagttgaa aaaatcgctt cgttaagaga agcgttcaac
    16861 tatatccaat acattctttt tacatttgga ttgttgtttt ctcttctttt gaccgttgcc
    16921 aatgtcgaat tagcaactat cgttttttct tcttcacagt tagctgtttc ccatgaacca
    16981 ccaaaagaaa tcgctggtga ggactatcat gtattttatc cagtaaccat cgggaaaaac
    17041 cacgtggatt ttatttattc caatcgtttt gcatcagcag cagatcagga attatatgaa
    17101 actttgaata aaaacggaag tatattagtc aatgtatcag attacttgaa tgaagaaaat
    17161 gaacagtttg gcagagggat aaaaataaat ctgaattatc ttaagaaatt tcctttgatt
    17221 gatgtctctg gaaggttgat ccaaataacc gaaaaagaaa cgcaccctgt tatattgatc
    17281 cctgaacgat atagacatac agatttaaaa aatgacctgg cgcaaataac agaatattat
    17341 caagaaatca gtgaaaaaga accgaaattt ctatttataa aaaacaatca acccatttat
    17401 acatttattc cttctatccc ctggatcgaa cattatccgg ttgttgaaat actgacattg
    17461 aaaaacagta tgtatttgga aagaaatatt ctcagcggag aaatctatcc accgttaaag
    17521 atcaagatag gctctttgtc gaaagaaagg ttatttgagc tgattgaaga aagtcagtta
    17581 cgtgataact tgcagttatc atttccgtat actcagactg aagagatagc tgtcaaagtg
    17641 ttaagcagat catttcatta cttgatacag atatttttac tcactttctt ttctttcttc
    17701 ttgttgagct atgtcatgtt atgcatatat tttgtctaca attgtcggaa aatcgctatc
    17761 tttcgttcaa gtggttattc gctctttgaa acgtataaag atttcttcat gatgaacctc
    17821 atcaaatggg gaacgacaag tgtaatcttc ctttttctta tagaaagaga gcctaaatat
    17881 ctttttaata tttttttctt tagcttcatt gattttattc tttcagttgt ttttattttg
    17941 tctacagaaa agaaaagtca gttattgctg atgaatggag gttaacaatt ggagccgttg
    18001 atagtgataa aaggtgcgaa aaaaatcatt cgaaacaaaa cgattcttca tttggatcgt
    18061 ttcactgcgg atgctgggga tcttgtgact gttgttggag aaagtggagc aggtaaaagt
    18121 acgttattga atatattagg attgaatgat cagttcagtt caggacaatt ctatttgttc
    18181 gatcaagaga cgaaacaatt aactgggaga gagaagcgct tgataaaaag aaataccata
    18241 tcttttcttt ttcaagattt tgggttgatt gaatatgaaa caatcgattt taatttagat
    18301 attggattga agttttcgaa tttaaatgtt tcagataagc gaacagcaaa gtatgaggca
    18361 ttgaaaaaag taaatataaa caagaagcta agtacgccta tctacacatt atctggtggt
    18421 gagaagcaaa gaatcgcctg tgcgagagtc ttattgaaga atagcaaaat cattttagcc
    18481 gatgaaccga ctgggtcatt ggataaaaga aataaggata aagtagttga aatcttatcg
    18541 caacaagctt cccaaggtac gttagttatc gtagtcacgc atgatccata tctgaccaaa
    18601 ataggaaaca agaagatcat gctttaaaaa tcaaaaagga aaggatgtcg ttttatgaaa
    18661 aaagcagtta ttatttgtgc actagggata ggttttgttg gaggaaacgc tatgtcatct
    18721 gaagtggaag caaattcagt tcacggtgaa atctattaca aatctttaaa gccaggagag
    18781 attcattttg atgctaaagg taatattatt aagtcaaatt taccagtaga ccgggaaatt
    18841 tctagaacag caattaatca tagctctgga agatgggttt attatagtga tacaacttgt
    18901 acgagaaaaa caggtcattc taaccactat agtcgtgtct ataatcgtca cggtgcaaaa
    18961 gcgaaagttg gaaactctat tcgatcagca aatgcaacaa aaggagtatg ggcctatgca
    19021 agtgcgacag gaaatagaac tgacacattc tcatgttggt acaatccaac cggttggtat
    19081 taagtatcca aaagtttata taaaatagaa aaaacatata atgaaacagc gttaaaagtt
    19141 atttttggta gttttaatat attcattttt agttgtataa aaatatatca taataaaaac
    19201 aacaaaaaaa tgtctggaaa atcgaacata aaagtgtctg atttgaatat cccagcagcg
    19261 gaagaagaaa aacaaaatcg ataacatttg ttctaagaat tagttacgtt aaatacggaa
    19321 cgcaagatag acttgaaaaa cggtaacaaa agaagtagta tattaaggtg tgatgtgcta
    19381 cttgacattt attctgctga gcgaactttc tggattgtgt agccttatat ctattcagga
    19441 cgtatcgagc agattagtaa aggtgttctt gatgattttc atcttgaaca cctttgtttt
    19501 tagcaattcg caaaaaacga aaagtaggag aattgataaa tgaaaaaaag atttttagca
    19561 cttgccatcg tactaggcac tggcttattg tcaggctgta cgaatgccgg tgaaaaaaca
    19621 gccgttagtt ataaaggcgg tacaatcagc gaacaagaag taatggatag cttgaaaaaa
    19681 atgcaaggag cagattccgc tgtgcaacag ttgatcgttt atcaagtatt tgaagataaa
    19741 tatggagatg acgtatcaac caaagaaatc gattctcaat atgatcaaac aaagaaacaa
    19801 ttgggtgatt catttgattc acaattgaaa tctgcaggtt acacagaaca aacattcaaa
    19861 gacagtatca aacaaagtct agcctttcaa gaaggtctaa agaaacatat caaattgacc
    19921 gatgaagatt taaaaacggc ttgggaatct ttccatccag aagtggaagc tcaaattatc
    19981 caagtagcaa gtgaagatga tgcaaaagat gtgaagaaag ctgctgataa gggagacgat
    20041 ttctctaaat tagccaaaga caagtcaact gatactacga caaaagaaga tggcggaaaa
    20101 gtgaaatttg attccactac tacaacggtt ccggcagaag tcaaagaagc agcctttaag
    20161 ttgaaagatg gtcaagtttc tgatgtgatc acttctacca atgcttcaac ttatactacg
    20221 gaatattatg tggttaaaat ggtgaaaaac caaaacaaag gcaacgacat ggataaatac
    20281 aaaaaagaat tgaaagaaat tgcaacagac acaaaattaa gtgacagtac cttccagaat
    20341 aaagtaattg gcgaagtatt gaaagacgct aatgtaaaaa tcaaagacaa agactttgaa
    20401 aatgttttga gtacattcac ttcagattct agtacagcaa gtagtaaata agcggtataa
    20461 ataaaaaata tgaactattt aaatcaataa aactgttcat ctaaatcttt ttattagaac
    20521 taaatcaaca gtagtttaat tactacttga tttagttctt tttattttaa aaattttttt
    20581 tacttagaat ccagcgaccc tagatatttt tctgtataat cttaaataaa gaaaacaata
    20641 tcaaaaaatg cacgtagaag tagataaatg gagtggtttt gcaaatttct tttatttcta
    20701 gaagttattc acgtatacgg atttgttttt ctcacaaata aaaaggcagg aagtacaatg
    20761 gatcatttgt atataaaata tattcatcaa ttttccgttt tggtgttagg cacactaatt
    20821 atagcgggga ttgtcgcatt tattattcaa ggccgtgaca tattaacagt acatcaagag
    20881 tttttcagtt atgcatttgg tattcctctg atcgctataa ggtctgataa caaaaagtgg
    20941 aagaaacgct attacattct cttgctattt ttgtgttttt atctgccacc taagattttt
    21001 actttattag gtatacaatg cctatcattt tcttttattt cctatatcat tgttttaatt
    21061 ttcctcagtt tcatagtatg gatgttttat cttgtcaaaa aataaatgaa tgtttcatga
    21121 acagtacttc atcacagcct aatttttttc tttatgctat cgttatatta aagaaatcaa
    21181 tcagaaggga aagaaagtga tgaaaaaagt tttattagtg gtaaacccca gttcaggcgg
    21241 tgaacaagca aaggaatttg agcaattagc gatagcaaaa ttggaatcgg tgtttgatga
    21301 agtggtcgtc ttgcatacaa aaaaagcagg ggatgcaaaa aactttactc gcgaagcggc
    21361 tacggaaggg tatcatagtg tgtttgtcat gggcggagat ggaacagtca acgaaggaat
    21421 cagtggaatc gctgaacaag aacatcggcc aaatttcgga tttttcccat tgggtacggt
    21481 gaatgacttg gctcgagcat tagggatacc gctagaacct gaggaagcga tcaatcattt
    21541 ttcgattgaa tcagtcaagc cattggatat tggaaaaata aatgacgact attttatgaa
    21601 tgtcgtcgca attggctcta tccctgaatc aatcaatgat gtagatgcgg agaagaagac
    21661 gaaatttgga aaactagctt atttcatgtc aggaatcaaa cagttagctt ccacacaatc
    21721 ctactcgttt cacgtagaag ttgatggaaa gaaggaagaa attgagagca gtaccttgct
    21781 gataggacta actaactcag tcggcggttt tgaaacatta cttccaaatg caaaagtgga
    21841 tgatggtaag ctccatttgg tttatttaaa agattcttct ttgatcgata cgcttaaaac
    21901 attgccagac ctattaaaag gagtagatga atctacgaaa aatctagcct accttacttg
    21961 tgagcaatta gttgtgtcat tagctgatga ggaagaattg acgacgaatg ttgatggaga
    22021 tgaaggggat aaattacctg tgacaatcaa agtattgccc tcacatttac aggtttattg
    22081 ttaaaatgga ggttgtgacc gaggagttgg cgggagaaca ccgcttgttc gggataaggg
    22141 actgtgacaa gacttttgtc acagcctttt ttttgtacaa aaaagaaatt tacacctaat
    22201 agaaaaattt ttccataaaa atacatattt taaatatgaa agtacaattg gaaatagatt
    22261 aaaacgataa aataaaattg tcaaaacgtc agagaggatg gtggagaatg aatcaatgtg
    22321 taaaaaagct gattgcgtcg attttgtttg tcttccctct cttgatggtg acatccgttt
    22381 ttgccgatgt agagaaaaca aacgaatcaa cgcaatcaga gaatcaaagt tttgacaaga
    22441 ctactttttc agaagaatcg attacttctg aaactacaga atctcaaagt gaggcttcta
    22501 ctaataaaag gcaagaagaa aataagacaa gtgagacctc agaagtagct gaacaaacgt
    22561 ctgatagcag tgaagcggca ctggcaagag ctgtttcagc ggatgggtac agcgaagctg
    22621 ccacaacaga agaactggcg cagcttctcg cagacgaatc agtaactaag atccgactga
    22681 ttcagccttt gactttagac agggaattag agatcaaacg agatatcgtt attgattttg
    22741 gtggattcgc tcacaatttc ggcacacacc atatttatat caatgagata cctcagtcga
    22801 tcgagttcca gaattttaaa ggaacagctg cacatccagg tggattgaca ccaaatatcg
    22861 atggaaatgc cattatcatt gcttatatga ccgatttttg gggaagcaga tatcatttta
    22921 cgggagaaat aaaatttacc gggacacttg atttgtatga tgggtccaaa ttaggactga
    22981 tttacgctcc tcgtgcaact gtcacattag atggtgtttc cggtgtgttg gatgtccaac
    23041 cagtgaagga aggactgaat gccgctccgg gtaaagccta tttttgtaga agctatcaat
    23101 tgaatgtgat caatggtagt caactttatg gaccgtatct agggaaattc tacggttttc
    23161 tagattcagg agatgagact ggtagtggaa aaacagcgcc aggaatccat atcttaagcg
    23221 gttcaaaggt ctctcttgat tatgatcggg cagatggcgt tagcgatgaa ggagaggcag
    23281 tcgatacgct acctggtaat gtcatgtttg aagtcagtgg ttcgggaagc gaattttcgg
    23341 taaatacaaa gatcaatatt accaatgaca acaatcgcgg aatcatccaa atgagaggtt
    23401 caggatcgaa agtcactgta tcgaatggtg ggaaaatcac catcaataca gcaactacgg
    23461 gcggatttcg cttgcaagga gattctagtc aaatccatgt atcaagtggt ggacaaatct
    23521 cggtcaaaat ggctggagat gacgatcaac ccgggaacaa tgggatgcgt tttgtaggaa
    23581 aaggcctttc tttgatcgta gaaggagccg gatcagttat cgacatcgat aagcagagtg
    23641 ggcgttccag cgcagttcgt tttgagaatg gtacgcaaaa gctgacagta accaatggag
    23701 gctcattgag ggtaagaaat acaggtgatg gcaaatctta cgtgaacaga ctcaatcaag
    23761 gaaatcaagc gatccaattt tatgacgctt ctggttttct agaaagtcca ggttcagctg
    23821 attttacagt cactggagaa aaatcaaata tcgatatacg cgcagattcc ggagcaactg
    23881 tagatacaac tggagatatt gatttaaatt ttacggcagg tgaaaaaact tatttagtca
    23941 tggtcgggaa aacaggggaa gataagaccg gcatcatcag tggggatgtg ttgaatgtgg
    24001 acttagaatc tcctacctat tttgatttcc aaaacaaacg gattggaagc gagaccaatg
    24061 gcataggcgg ctgggtattc caaggaaatg gagattcttc tttaaagatc gcccattcag
    24121 aaatcgcttt atggcgaaaa acaggcgcaa cattcgataa ctttgaaaaa gacccggatt
    24181 atttttctga tatgacagac ttatcgatca atgggaccga cttaggcaat ttcgtatcaa
    24241 gccctagtgc aggtttaaca aatgaatttg ctcagacagg tgccggaatg aaaaattata
    24301 atcgaatcag tgccaataat cagctcccga tgattgaatc cttaagagtg ccaacgaatg
    24361 cagataaaaa aatctatggg catgtcgttg ttcccgaagg tgtcgaggca aagccaagag
    24421 atgcttggac aggcgaagta gaagtgactt tacgtattac ttacgcagat acgacgaaat
    24481 catctgtaga aattactgct ctcacgcaag gacaaacaga tgaaaatgaa ggggcaggat
    24541 ataacccatg gggagaaggc gaacaaggcg gcttgttcga agttcaagta cccaatgatg
    24601 agagattaaa aacggaggat caagtgcaag ttgttgctgc taaaaaagta agaggtggcc
    24661 aagaagtgac agaactggac tctgccaaaa cgacagtcga tgtgattcct cctgaacctg
    24721 ctaatttaag taccacattg atcaatgcag cttctcgcac gatcacaggt acaggtgaag
    24781 caggagcctt ggtttcattg aaaaaaaatc aagctgtttt gtcagaaaca actattgatg
    24841 atacagggga atttgttctt tctatcccag aaaatcaact ttttgcagga gatgtattag
    24901 aaattgtatt aaacgaccaa gcaggagaag ctactgctaa gggtgtgcag aacaagccaa
    24961 gtaccaacga tgaaattggg aatcacaatc caagtgatac gccagttgtt tatcatgatg
    25021 ccccgccttt tgcacctgct acgaagatcg tagtagaagg tggtctctca tttcttgccc
    25081 cttccaaact cgattttggt cagatcaaag caactggttt gacacaagaa gcatttggcc
    25141 aaatggatat acaggaaaaa ttatggattt atgatcagcg agaaacgaaa aatccgtgga
    25201 tcttaacgat aaaattatct cagcctttca caaatgatgc aggattttct atgcaggatg
    25261 tcctatattt caagaaagga gaggagtatc aattagtgaa tgaagaagaa ctggaaattg
    25321 tcagaggaca aaattcaacg aatgtgcaag cagattattc agaatattta aataacggtc
    25381 gggcgtttaa acttgtctta accaaagaaa atcagatagc tggtaattac tcagcagaga
    25441 taacatggac attagcagat gcgccgacag aataaagatc aaggaggatg attgatgaat
    25501 cgaatatgga ataaaagtca tcgaaacaac tgctctttac ttatgatcct tctccttgga
    25561 ttcatccttt ggcagacacc tgtccatgcc tccgaattca attttgcggt agattttgaa
    25621 ttaccggcta atcagattga taagaacaaa acatatataa atgtaaggat ggagcctggg
    25681 aaaacacagg aaatccccta cacggtcttt aaccgaacag ataaatcagt aacagttgag
    25741 acattgatcc actccgctac gacgaattca aacggtgtga tcgaatatgg agaatcaaaa
    25801 agtaaatttg atgaaacatt aagttatgat atgaaaaact tagcaaaaac agtgggagaa
    25861 ataactgttc ctcctcaaag tgcggtcact gagacacttt cactgaaagc gccagaaaaa
    25921 gagctggctg gtgtggtcgc aggagggatc acgtttcaag aaaaaaaaga gaacgtttct
    25981 aacaaaagta cagttggtgt cgtcaatcag ttttctcaag tgaaagcaat cttgatttga
    26041 aatagcgaaa aagagtcttc cccagatttg aaattaaaaa aagcttatgc ggatcaaagc
    26101 gatggaagaa atgtgattaa ggcgaatctt caaaatccaa atgcttctta tatgttcggc
    26161 ttgactacat cggttgaaat ctacaaaaaa gaaacaaaag agccttacct ttcaataaaa
    26221 aaagaaaaaa tagatgtagc acccaataca aattttgatt tacttgtccc attagaagga
    26281 aaaaaattag ctccgggaca ttatcaagcg gttgtgaagg gtgcttggaa agaaaaacgc
    26341 tggaatctcc ataccgattt tgaaataact gatgatgcag cggaccaatt gaatgaaaag
    26401 gatattgatt taaaaaatca gccagaaaca tttgactggc gatggctgat acttgttgga
    26461 gtcattctta ttggcttatt tgctggatta tttattttaa aagaaagaag gaaaaagaaa
    26521 tgaaaaaaac aattgcatta agtaccgttc tagctggaag tctattatta tgtacacaaa
    26581 gtgtttttgc tgagaataag agctcagcca ctacagaagg aacgatttct gtgaccaaac
    26641 cagatgctgc aaatcctgaa gacgttgttc cagttcctcc tgttgatcca aatgatcctg
    26701 atactccttt tacacctgac gaaccaaatc agggaacatc cggattactg acgattgacc
    26761 aagctccaga tcttgatttt ggaacaatca agttaggtac agcaaaaaca gtatatgctg
    26821 cattacaaag cggaacggac agtactggag cacaaacaca agtacagaat ttcgtacaag
    26881 taacggataa aagcggaaat tatgccggtt ggaaattatc agtcaaacga acagaattta
    26941 ttggtacaac atctgatacg aataaactaa ctggttcaaa ggtgaaattc aaaaacgccg
    27001 tgattcgaaa tagcgcaact aatagcagtg gaatgccatc taccgtttct ggaactggcg
    27061 ctgaaggaat cgaaatccca gcagatcagg cagtagatct agtaactgca ggagcaaatg
    27121 aagggattgg cacatggatt tattcattag gagagaatgt ggaacaaggg agacaatcag
    27181 tggaattatt tgttcctgta tccaattatg ctgtagacaa ctacgtttca acattcacat
    27241 ggtcgttaac agatgctcct acagaagagg aagcgtaaaa tcatatcaac gacttcaggg
    27301 agtggagccg attcaaggcc cactccctga aatctgctat gcttacgtct cagctcttat
    27361 tcatcaactc gaagcagttt tttggaagtc atggaaacgt tcctttgtct tttcaaagat
    27421 ctttcgacca acgagtaagg agtcaccgtt gaaaaattct aacacataat gagcagaatc
    27481 aattttctgc aattgcgata agttgataca gtaagatttt aaaagtaaaa agtaagcata
    27541 cttttctaac tctgtcttca attgttttaa agaagtattc actaatttat cttctgtagt
    27601 tgttttgacc ataacttggt ttttcaaacc tttgatactt tcaatatata aaatttcccg
    27661 aggatacagg acgagacttc agtcaa
//
LOCUS       DAZABD010000037        27373 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000037 DAZABD010000000
VERSION     DAZABD010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27373
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.037"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            160..3735
                     /locus_tag="ACHALG_001641"
     CDS             160..3735
                     /locus_tag="ACHALG_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFN2587033.1"
                     /translation="MISNKKRWLKVALLVAFCQIAFLASMTTQAEEIVQETTVSQVSP
                     TSNEVEVQSTLPKEAVKEETPSEVVPETEAPSTNEKTVLDETIGKSQQPGDSPVVAEE
                     TQVEAHEAQAIPEKEKSLEESGSTEINEGTEPVQLEDSKEVTTPIESTKEEMDSNTSL
                     SSEKEEKTVLLTEPREPELKKVEEFVDTKKEAVSPTSSKEDTLIVQQTARSARVRRAT
                     NTAVLGGRKIDWGENIGVISSANNPNFTLNYMVRFEINGQVAFCIEPGVAAAEGQVYT
                     ATALENYLKDLNIRKKTSLISYLGYVTSKDKSDEQYIAAQFMIWEVLGTKIPIKNIRI
                     DYDRRKAEIQRLLNNFDVRATFHNQTHTVKIGETLKVKSGNDFLQRVKDIQKPKGVNV
                     SIHDNEVWITITKEAPEKAKIHFNQIEALGVPLAYRRPGAQTIGVLNPFVPGDSVVNL
                     TILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQANGKKIKEITLKNINGKVQAEI
                     KDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNTIAKDQVIKGTIDLVKVANKD
                     LVDSTNPDNKPKLAGIEISLTSKTTGKVVKTVVTDNDGYATFGKNTVVFDTYILSETK
                     GKEGYKLFEPFEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKVIALAGTQFKIW
                     DKYANNGKGGYVSMALPNDTEVSDIFTTNEKGYFVTTDTLKYGKNRYELREIKAPEKY
                     VLNKTPYVFSVTDDTNTIQLIYFANRLAKANVEVFKYDTSTNFDQKTAIAGVEFDLFK
                     KDETGTTFVGTFKTDKKGKINVKDLLVGDYFFKEVKPLAGYLALDKDVPFTVTVDKDG
                     TVIALEVENKRIPPKIGTTAIGKYTNQQKVTPLKKVTITDTVKYSNLIIGKTYTVNGV
                     LMDKEESITAGKDVPLLVNGKQITASKVFVAKNPNGMISLDFVFDASALAGKEIVVFE
                     NVYRDGRLVASHTDIHDKGQTVRIVNPMIHTKATGQNGEKQLQALPNQKVKEWVQLND
                     LIVGQTYKVLVQGYITPDGVKLDGTSAEKIFKADKTTLEFLFEFLVDGRKLAGKGLSF
                     AEWLEAQSEPKDENDYEVRFEQIANHNVDLKSEDQTVYFVEVPKVPEPKIPEKSQPFK
                     EVKQASVVPMLPKTGSEDTVLWMIIGVLFVLAAGLTFYELRKVKD"
     gene            3835..4773
                     /locus_tag="ACHALG_001642"
     CDS             3835..4773
                     /locus_tag="ACHALG_001642"
                     /inference="COORDINATES: protein motif:HMM:NF012948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFN2587034.1"
                     /translation="MKKKERKKRMKKLFFACLFSSVVLAGGINVFADEIGITPVTPPV
                     IEETTPVVPIPDISKIDESVPDQPTPVDPDISSEVTEPTTPVVPDITENSENETPVVS
                     PSTGETTNDKDTTPTTSESYSGTSSVDNDSKIDDFSSSSTTIDTPKETEKKNDLGAPK
                     NLTPENHIKTVSEKEMTVSVSSDGKIVQGTGTEKSIPIVTSNLEEINHVPTPETPLQV
                     AIGQAIGGVQDGVPLVQDNQGNLVEDLSIPVKKLPSGNIEIKTIDGKTKVLPKTGEEL
                     QISLTILGSLLTAVTGGIFYGKKQWRHLRHMFKKTI"
     gene            4790..5140
                     /locus_tag="ACHALG_001643"
     CDS             4790..5140
                     /locus_tag="ACHALG_001643"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587035.1"
                     /translation="MGLSYVAISQEEVLGKCFNQKHVGSTGERDRRGNMKKVEWEIFQ
                     MNSEKMGEIEVRVAVGGAPKAMRYNAEVLLANPRISRRADVRNIGNDGETNKIARNRF
                     YLLVDRVEVKGGND"
     gene            5140..5571
                     /locus_tag="ACHALG_001644"
     CDS             5140..5571
                     /locus_tag="ACHALG_001644"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF961 family protein"
                     /protein_id="HFN2587036.1"
                     /translation="MALEFDQGIVSGLVKETLGEIYFVKAQRPRMLYEDNENTGEIKE
                     RPVEFSSAAQQTTFVVNFPPETNLDELNFGDVVELEEVEFRFWISIDRESMSNRAEND
                     LKVSALRYKKTGKNVFQTSSSTRATYVKEKQDSNKESAKKE"
     gene            5839..7158
                     /locus_tag="ACHALG_001645"
     CDS             5839..7158
                     /locus_tag="ACHALG_001645"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFN2587037.1"
                     /translation="MFQYKGIRIRNRHKYASERFIFYLMLPVLLLMSGFFSLFYTTIT
                     WEWCCIYWIIAFGLAGLFAYMCLYFYFYNVYQRLVHKGKLAKMIHSRRLILERVLKNG
                     RRKVVYYPKIFYHYKNGLIHVRLPLDLGPFQDRFNEMAGQLEEAFFCDLVEEQREKNY
                     ICYTFLYDVEKNRIGIRDITVKDGIITLMKNVQWAFNKAPHAIIIGSTGGGKTYFIMS
                     LIYALLPYGTFDICDPKRADLASLEKIPIFKDQVFYGGGILSCLINLAKETANRYDFL
                     REQPEYELGYDYTYYHLTPHFLVIDEWAAYYASLDTKEKKKAMGCLNQIALMGRQCGV
                     FLILGTQRPDTKYLDGAIRDNFGLRVTLGRVSKAGYKMIFEHTVKSLFNKPIKGRGYI
                     DSGTDQVREFYAPFIDITRFNFFAEFKQLSDQLMAEKIKETKEGKNV"
     gene            7151..7315
                     /locus_tag="ACHALG_001646"
     CDS             7151..7315
                     /locus_tag="ACHALG_001646"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587038.1"
                     /translation="MYEEIPATCGTRLGIKDLMIDSFLAIVFSEKRLSFFINKKVENR
                     WRLERMYPTS"
     gene            7854..9029
                     /locus_tag="ACHALG_001647"
     CDS             7854..9029
                     /locus_tag="ACHALG_001647"
                     /inference="COORDINATES: protein motif:HMM:NF014537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFN2587039.1"
                     /translation="MDGTQLKQMRLSRNLTQKLVADTLGWPQSFLSMIENDQRSCSKE
                     QEEALCEFYANFSSFTSLEIKFDYVRVRVPTNDTKTVVEEILGMDFNSFFYAETRLYG
                     YIEMYQKGMIRVLNSKKGDDKGVLIELSGQGCRNYESVLDERNDSWYQFFTRCVSFNG
                     VPRRIDIAVDDFEELFSLHEFAQKLNRGEFDSKFRTWRFQEEKQLLDNLSSGLSVYLG
                     SSQSLMYFCFYQKNYEIAKKQKLPLEEVEVKNRYEIRLRDAKAVSFIEQYLNKVDFSD
                     LTMGILANQLIMFEGEKNANVWKPWGKLIQNVGKVNLTITPEKPNYNRKLMYLKTQPA
                     RTMKIVKTVDEILGLNRFGEILEETELREQDEKIVEDEVRAVYEYIQENPDAIHYLL"
     gene            9533..9730
                     /locus_tag="ACHALG_001648"
     CDS             9533..9730
                     /locus_tag="ACHALG_001648"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587040.1"
                     /translation="MGQYFLTGVWYVLKLVIILGIAYICFRILLSGEGMPTPKDFIIS
                     VLNFVADQIQVIIHWAQQRFG"
     gene            9772..9900
                     /locus_tag="ACHALG_001649"
     CDS             9772..9900
                     /locus_tag="ACHALG_001649"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN2587041.1"
                     /translation="MIENFEYKLSRLPKKKGATQKELANKLGVNVQSVSNVEEDKS"
     gene            complement(10125..10673)
                     /locus_tag="ACHALG_001650"
     CDS             complement(10125..10673)
                     /locus_tag="ACHALG_001650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587042.1"
                     /translation="MAILQPFGEPIERTEFIQHYMKLFVQVIKHTHQIDEFYSKEIEY
                     LLAEKQKIALLYDYFVEMYDRAPDYFYLSDTLTTNFLAKEYLFASHTKNFMCVEHFVN
                     TYLHLLKTQKICTFEAFQTDYLFILDREAYHAKQAFEKQNQAIEGYPELRIQNNSFLQ
                     QRLLKQLINGFHQRNKGYQKDQ"
     gene            complement(10728..11021)
                     /locus_tag="ACHALG_001651"
     CDS             complement(10728..11021)
                     /locus_tag="ACHALG_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587043.1"
                     /translation="MLRTNAIPIVDSYDRNTNNYLGSFEQTDENILNYVAGLSPFQSV
                     RLVEHTTDTLILTTIGYFFDHVSDQQWLQQILPKLIAKQTGKKTIEAVKIFFY"
     gene            complement(11221..11562)
                     /locus_tag="ACHALG_001652"
     CDS             complement(11221..11562)
                     /locus_tag="ACHALG_001652"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587044.1"
                     /translation="MKANTPYKVGDIFYSSWGYEQTNVNFWQIIKLTEKTAWFRPIKK
                     QTVKTYTSMSGETMPIPNEFIDYPLARTKDSIVRKRINPALYPNELYGNRSWDTFYRY
                     DNQPVYESSYY"
     gene            12063..12212
                     /locus_tag="ACHALG_001653"
     CDS             12063..12212
                     /locus_tag="ACHALG_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587045.1"
                     /translation="MKKKVIPYCPKPKNAFERGLFKYFEGKKGQDHKEENQKKKSKKK
                     SEEFF"
     gene            12487..13287
                     /locus_tag="ACHALG_001654"
     CDS             12487..13287
                     /locus_tag="ACHALG_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFN2587046.1"
                     /translation="METIIQIMKKYDFSEMETRVYTTLLEKGNLTGYEVSKISGVPRS
                     KVYNILEKLLKKNLIVVNKSEPKLYHAISANEFLEKLEKSVKNDLSFLTKNLGMIKEK
                     DEEEMLWKVDGIEYVLDKVEHLVKNAKESLLIQVWHENLTDSLLKTLQQAEKRVDKFV
                     LILFSSTHEYDLPLEKYYIHGFETDKLADFGARWINIVADEQEVVFGTINEELQSTDV
                     TWTKNHAMVNLAKEYVKHDAYTLKVIAESSEELKAKYGEDFEGIRKIY"
     gene            13303..14181
                     /locus_tag="ACHALG_001655"
     CDS             13303..14181
                     /locus_tag="ACHALG_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFN2587047.1"
                     /translation="MIRNILLTLIVAINLYFIIYFLRDLFKNKQNFKEEPGDMRLLPF
                     TSFIIFFLSTFGVSDFAIGTVLYPKLKWVSMKKLPGTLNTQCVVPVAVMALSYITAIN
                     VGIKTLAVCIICQIIGAYLGPRFVVKLPEKTIKVFVGIGLVIAALLIFAGQMNWIPSN
                     GTASELYGGKLILAGFLLFVYGALNNIGIGSYALTMVTVYLLGLNPVAAFPIMMGACT
                     FSVPIGSVQFIKFNEYSRKITLFTSTFGVLGVLVAVFLVKSLDTYVLKWIVILVLLYS
                     AYTMLGSQLKKVSAAN"
     gene            14194..15174
                     /locus_tag="ACHALG_001656"
     CDS             14194..15174
                     /locus_tag="ACHALG_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFN2587048.1"
                     /translation="MLFLKKEDIIKSFSMREAIDADKKALSLYSAGKASVPLRTNIDV
                     PKSNGQSLYMPAYVEGGEGALGVKIVSVYPENIKKNLPSVPATMIVLDPETGMVSACL
                     DGTYLTQLRTGAVQGAATELLAREDAKIGALIGTGGQAQSQLEAMLTVRKLEEVRIFD
                     IDFDRASRFAEEMMQQFSVTMRPTKTSQECVEGADIITSVTTSKRATFSAEWVKKGAH
                     INGVGAYTPEMCEIPREIIKAADVVIFDTMDGVLKEAGDFISPLQDGYIQRDSYHGEL
                     GQLINEELIGRTSGEQITIFKTVGSAVLDVVVATEIVNKAKENNLGKLIY"
     gene            15696..15914
                     /locus_tag="ACHALG_001657"
     CDS             15696..15914
                     /locus_tag="ACHALG_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587049.1"
                     /translation="MNFFQNVFTDFVGDFKYLIMLAMLGGAAYIAFERKVSKAIPLIL
                     VLAVAVWLVGDTSGVFNWILDRMRSWGR"
     gene            15916..16323
                     /locus_tag="ACHALG_001658"
     CDS             15916..16323
                     /locus_tag="ACHALG_001658"
                     /inference="COORDINATES: protein motif:HMM:NF024062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpE family conjugal transfer membrane protein"
                     /protein_id="HFN2587050.1"
                     /translation="MNKIRNWTKIWYIERVFYKLWDGVALPRPVTQTFAIWFGIGFFG
                     GIFRIFPFVMEHTITRQFFCPWLFAFLMTRKQFQGRKPYNYLASFLLFQCRSKESFHG
                     QWVKPIEKKEIKRGIRCVVRYGGEKDATTKTSA"
     gene            16298..18835
                     /locus_tag="ACHALG_001659"
     CDS             16298..18835
                     /locus_tag="ACHALG_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFN2587051.1"
                     /translation="MRLPKLVPKIPFYYLEDNLVFGEEDRVYAYYEFPMYSYSFVGED
                     QAYQLQQAIMRLIRQSHSSHVRFFFCTNEEKIEKTIKASKELVRSEGELKTIAYQHLE
                     GVQHILEELHGKYIQNVCWYVGIELVLEESAPKEAHWMKEFRIATKDFFHRSNARLFG
                     DFETIPNEKIQRYKRLENLLYNRISKYFRLRKTTPEDMVFLVEHLEGKKEQSLDEVMY
                     RPNSFKEETNTYTYHYDVLNLASAGITEKKNYLILEQGTEKEYITYLSLSRMTRDLVF
                     PFQSEIYYFLQSELDFPIDSWLDVNLLDNRLALAKVRGKKSDLSDIYESGMSVGKDVG
                     NNVREAYQSANELEQKLEDTREDLYVLSLNVRVSGKTYDEMLKRVSDVRNFFDVYSMY
                     LEVPCNVQLFLHFGSYPAGRRVIEDYRQYVGAEFIASLGIGATQQLGDGCGIPIGFNA
                     ETGIPVFIQPWLAAQGIKGTVTNTLAKALIGSLGGGKSLTENLLLFWSVLFGALAFCI
                     DPKSERSNWSEDLPYFAPYLKTVNITSEKENIGLLDPFQLMRRKKDQESLALDVLTYI
                     TGVSIRDEDRFPLLQRAVKTVSDFEGTKGLLLVIQELYAQEAAIAQRLAKHIESFKEL
                     SIAGLLFGDGTNQQGLNLDAKCNLALVQDLTLPDSETAPEDYNSSEILSIAIMMSLAT
                     YALDFIKQNRDIYKAVGLDESWAWLNVAQGKILGNKLVREGRSRNAGIDFSTQNTDDL
                     GDEKMRSKIGLKFIFRSNDREEITKALNFCGISVTEENIQRVKNLENGECLFCDIHGN
                     IGILYVYYWFEDLFHAFDTRPPMEKNYEEEEYKDEILEKLVEEIEVN"
     gene            18836..20857
                     /locus_tag="ACHALG_001660"
     CDS             18836..20857
                     /locus_tag="ACHALG_001660"
                     /inference="COORDINATES: protein motif:HMM:NF046089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFN2587052.1"
                     /translation="MKQWYSKVSFSITRRKLVFISLVIMMGVFFFHPIFVEAAGWIKE
                     TLGGKNAVYDFETYPLDNYNLDFFVDTSWNWLPWKWGEGISNTVIYGIFAVTNILWML
                     NVYLCYFLGFLVQEAFELDFLSELIGSLSHSIQNIAGMNRAGTLSHGLVPFFGGLLIA
                     LAGCSVVYQGIVKQHPTQAIQQAVTFLFTFICCGIFFMNTHTYLEKANEVQKEINSEM
                     LTIAKDLLPEQKGKPDAIKAIRENLFAIQIKQPWLVLQFGDSDIKTVGEERVINLLKE
                     SPYDEGEQRNKLVEEEVTKQKNGNLSVQKVYLRFGMVFLTLIVNIVISMGVLALVVIL
                     IASQVLFLLFIGFLPVAMIFSLFPNSGSLLGNALQKVFQLLFTKMGILLILTITFSIS
                     HELNHLSKNKGMIWTSFLQVTLWFSVSTRVNELLGWMKIGGAQTRAGDRTGRFLRGMM
                     LGTMGQKMIRGIGENFVGGAAGGVADQMISKSMSGQRSITTTPLIERVGKKVSAMTEL
                     PAMIESRAEQWKRTIQYVPTNAQYKAQKMKYDYFSGRMNESERQTRARNLANERQKNF
                     YEARKDALQLEQQERKQQAMRSMAHQTGKTQVTSGKLQTRKSAMIGRTEQFPTIEKRS
                     NISNVAKNKKLSKQEHPIHERSTNRKIETPVRWTSTSCQNARKGRTKKE"
     gene            21004..21792
                     /locus_tag="ACHALG_001661"
     CDS             21004..21792
                     /locus_tag="ACHALG_001661"
                     /inference="COORDINATES: protein motif:HMM:NF024056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFN2587053.1"
                     /translation="MVFSVCFGVYNNLTSQDTHTIHETKVIDKQLKDMTGIESYVKNF
                     AFLYFTVSLESDQQINRSKAVESYLADGLQMDNEIDKDIETNVKVKNVSIWAIEEHES
                     KKHTFVVIFHVLLEIEKEFKERVYKVEVYRNELSYAIVKLPIVASNVKKATIKVDTRF
                     ETTSISRKEQEELEKFLYTFFRIYPKANAEELKYYGTTLLPIQQPLAFEELSNLQVAK
                     KEGRYEIHCNILYQDQETHLWFTMYYEMSMKQLENGNFRIEAIH"
     gene            21825..22883
                     /locus_tag="ACHALG_001662"
     CDS             21825..22883
                     /locus_tag="ACHALG_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002386462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HFN2587054.1"
                     /translation="MMSCLFLLFAIISMSSGESKHMDSAVGVTHTGEYIETLPIFSEI
                     KGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRNDTNFFGITWFPG
                     CPYPQGSARGIGGSEGGFYMKFPNLNACYDYYGFMVASQSNFNQCVGNKDPGSCLLIL
                     GRGGYAAAGITEGSPYYQGAMSIIKSYNLTEYDAFAIKHWNSLPTKKTSLIAGKSKGN
                     AKVLDRIIGRPVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGEDYNWATYGWQVI
                     MNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSNSGQSFSTVEQNAEQGRIMAR
                     YQRSYNQAKIRSLVRKVK"
     gene            22934..23446
                     /locus_tag="ACHALG_001663"
     CDS             22934..23446
                     /locus_tag="ACHALG_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF0163 family protein"
                     /protein_id="HFN2587055.1"
                     /translation="MKNYYFFFLSCFCLFGLTSCGWIFSEEKTEVSLISKEKMIQAES
                     SSKESKRNQEDKTECNISEEIQYLLKNFGRKWVNYTDIYERNQSVKNYLTDRCIKENG
                     IDNDPHVQYKTIGRIHTITQDVIQSDQYLLFGEETTNEMTRFVLLQIKVDQEVQKIDK
                     IKIYYVRSAY"
     gene            24607..25167
                     /gene="amaP"
                     /locus_tag="ACHALG_001664"
     CDS             24607..25167
                     /gene="amaP"
                     /locus_tag="ACHALG_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFN2587056.1"
                     /translation="MRKRLKLLLILVLFFVFFPLAHTLITMQSVVSLPFYLLPFTSYP
                     YIGNSMSQFLFWVSVCLLILLFIILIIVLFYPKANNYIEKKTASGKIAIQKKAIENFV
                     LIAAKEEPFIVDPTVKSVIKKKKIKIYIRGKIRKAFQTQERQTAFIRKVNTNLNELFG
                     AEQTILTEIIFKDYHEKNTNTKSKVL"
     gene            25177..25359
                     /locus_tag="ACHALG_001665"
     CDS             25177..25359
                     /locus_tag="ACHALG_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFN2587057.1"
                     /translation="MAEFISKYKYPFIFGCLGLLLGILIFTLGFIKTVILTVLTICGF
                     YLGNYLEIVGFFDKQN"
     gene            25415..25993
                     /locus_tag="ACHALG_001666"
     CDS             25415..25993
                     /locus_tag="ACHALG_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFN2587058.1"
                     /translation="MENKTNNTKTEIKKNDVAKTSEIIKGELVFEDKVIQKIIGIALD
                     EIDGLLTIDGGFFSNIAGKLVNTDNTTSGVDVEVGKKQVAVDLSIVAEYGKDVTTIYD
                     KIKEVISKEIKKMTGLDVIEVNVDVVDVKTKEQHENDSVTLQDHLSDAASTTGEFASK
                     QFEKSKEALGVASEKVSDGVENVKEASEPRVE"
     gene            26335..26982
                     /locus_tag="ACHALG_001667"
     CDS             26335..26982
                     /locus_tag="ACHALG_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN2587059.1"
                     /translation="MYKAANDLFNRLKLEKKELILKYILDEFGRVGYEATSVRKIASK
                     ADLSVGSLYQYFNNKEGMLTVAIEHVFVILDEYVSTINDAPLKLKDRLEIMFEMILKL
                     GKQHPELIIFYNKIPSDKKMITEWVKQFYNNRSFFDVYWSTVKELQNEKEISTKIDPV
                     IYTFIIDTMMLSGELLQNTQYQEIKAKVFLEDSIKSSLILKENVIETLTVLAKGQ"
     gene            26987..>27373
                     /locus_tag="ACHALG_001668"
     CDS             26987..>27373
                     /locus_tag="ACHALG_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN2587060.1"
                     /translation="MSTNKKDNKKEEIIFTAMKHFSKFGYEKTKLLDIANEAKTSTTT
                     VYSFFKTKHDLYEAAVEYDLNIINFELERIVSESSTLDEYIINIIERVRLKSDKKQLL
                     LNFYMLVSTNSVQIKNLEIIEEFERKK"
BASE COUNT         9606 a         3870 c         5490 g         8407 t
ORIGIN      
        1 ataaatactg ataaaagttt tatagacaaa gatgttcata gcgaacatct tttttattta
       61 ttttgagaag aaacagagca tgaaatgatg tcttatttcc atccatatag tctatttcaa
      121 acagaagaga acaattaaaa agaaaggaga tttcttgtta tgataagtaa caaaaaacgt
      181 tggttaaaag ttgctttact ggtagctttt tgtcaaattg ctttcttggc ttcaatgaca
      241 acacaagcgg aagaaatagt tcaagaaaca actgtttctc aagtttctcc tacttcaaat
      301 gaagtagaag tgcaatctac tttaccaaaa gaggccgtta aagaagagac accatcagaa
      361 gttgttccag agaccgaagc accttcaacg aacgaaaaaa ctgtactcga tgaaactatc
      421 gggaaaagcc aacaaccagg agatagtcca gttgtggcag aagaaaccca agtagaagca
      481 catgaagctc aagcaattcc tgaaaaggaa aaatcattag aagaaagtgg ttctactgaa
      541 ataaacgaag gaacagaacc ggttcaatta gaagattcaa aggaggtaac gactccgatt
      601 gaatcaacga aagaagaaat ggattctaat acttctcttt cttctgagaa agaagagaaa
      661 acagttcttt tgacagaacc tcgtgaacct gaattgaaaa aagtcgagga atttgttgat
      721 acaaaaaaag aagctgtttc tcctacctct tcaaaagaag atacattgat tgtacaacaa
      781 accgctcgaa gtgcacgggt gcgacgtgca accaatacag cagttttggg tggtcggaaa
      841 attgattggg gcgaaaatat tggtgttatt tctagtgcca ataatcccaa tttcacttta
      901 aattacatgg ttcgttttga gattaatgga caagtagcat tttgtattga acctggtgtc
      961 gcagccgcag aaggtcaagt ttatacagct actgctttag aaaactattt gaaagattta
     1021 aatatacgaa aaaaaacaag cttgatctct tatttaggat atgtcacaag caaagacaaa
     1081 tccgatgaac aatacattgc ggcacaattt atgatttggg aagttttggg cacaaaaatt
     1141 ccaattaaaa atatccgtat tgactatgat cgtcgaaaag cagaaattca acgtttgctt
     1201 aataattttg atgtaagagc tacttttcat aatcaaacac atacggtaaa aattggcgaa
     1261 acgttaaaag taaaaagtgg caatgacttt ctacaacgtg taaaagacat tcaaaagcca
     1321 aaaggtgtta atgtatctat tcatgataat gaagtatgga ttacaatcac aaaagaggca
     1381 ccagaaaagg caaaaattca tttcaatcaa attgaagcat taggcgtgcc attagcgtat
     1441 cgtcgtccag gtgctcaaac aattggggtg ctgaatcctt ttgtacctgg tgactctgtt
     1501 gtcaatttga ccattttgaa acaaggaaac attcgtgtct ttaaagaaga tgcagaaacc
     1561 ggtgtgaaag cacaaggtgc agcttcttta gaaaatgcag tgtatggctt gtatcaagca
     1621 aatgggaaga aaatcaaaga aattacattg aaaaatatca atggcaaagt acaggcagaa
     1681 atcaaagatc ttgatcctgg taaatatgtt ttaaaagaga tcaaagcacc aaaaggttat
     1741 gtactttctg atgaagcaat tgaaatcgtc gttgaacctg gaaaaactgt caatacgatc
     1801 gcaaaagatc aagtgatcaa aggaaccatt gatttagtca aagtcgcaaa taaagattta
     1861 gtcgattcaa ccaatccaga caacaaaccc aaattagctg gtattgaaat ctctttgacg
     1921 tcaaaaacaa ctggaaaagt ggtgaaaaca gttgtgacag ataatgatgg ttacgctact
     1981 tttggtaaaa ataccgttgt ctttgatacg tacattcttt ctgaaaccaa aggaaaagaa
     2041 ggatataaac tatttgaacc attcgaagtc acaatttctg aaaatggaca aaccttccat
     2101 tatgttttag aagacaaagt aatcgaacaa cgtcttaaag tggtgaaagt ggatcaagaa
     2161 acagggaaag tcattgctct agctggtacc caattcaaaa tttgggataa atacgcaaac
     2221 aatggtaaag gtggctatgt atcgatggca ttaccgaatg atacagaggt ttcagacata
     2281 tttacaacaa acgagaaagg gtactttgtc acaacggata cattgaaata cggaaaaaat
     2341 cgttacgagt tgcgagaaat caaagcacct gaaaaatatg ttctaaataa aacgccttat
     2401 gtcttttcag taacagacga caccaatacg attcaattaa tctacttcgc taaccgttta
     2461 gcgaaagcca acgtagaggt tttcaaatat gatactagta cgaattttga tcaaaaaacg
     2521 gcgattgctg gtgttgaatt tgacctattc aaaaaagatg aaacaggcac aacctttgtt
     2581 ggtacattca aaacagacaa aaaaggtaaa atcaatgtga aagacttatt ggttggcgat
     2641 tacttcttta aagaagtgaa accattagca gggtatctgg ccttagataa agacgtacca
     2701 tttactgtaa cggttgataa agacggtaca gtgattgcac tagaagtgga aaacaaacgg
     2761 attccaccta aaattggtac tacagctatc ggtaagtata caaaccaaca aaaagtgacc
     2821 ccattgaaaa aagtaacgat tacagacact gtaaaataca gtaatctaat catcggaaaa
     2881 acttataccg tcaatggtgt tttgatggat aaggaagaat ctatcacagc aggaaaagac
     2941 gtaccgttgt tagtaaatgg taaacaaatt accgcaagta aagtgtttgt cgcaaaaaat
     3001 cctaacggaa tgatttccct tgactttgtc tttgatgcta gtgcattagc tggaaaagag
     3061 atagtcgtat ttgaaaatgt ctatcgtgac ggacgcttag ttgcgtctca tacagatatt
     3121 cacgataaag gacaaacggt tcgtattgtg aaccctatga ttcatacaaa agcaacaggt
     3181 caaaacggcg aaaaacaatt acaagcttta ccaaatcaaa aagtgaaaga atgggtacag
     3241 ttgaacgact taattgttgg tcaaacgtac aaagtgctcg tacaaggtta tattacacct
     3301 gatggtgtga aattagacgg tacaagtgct gaaaaaattt tcaaagcaga taagacaact
     3361 ttagagttct tatttgaatt tttggtagat ggacgtaagc tagcaggcaa aggattatct
     3421 tttgcagaat ggttagaagc ccaaagtgaa ccaaaagatg aaaacgatta tgaagttcga
     3481 tttgaacaaa tcgcaaacca caacgtagac ttaaaatcag aagatcaaac ggtttatttt
     3541 gtagaagttc caaaagtacc ggaaccaaaa attcctgaaa aatcacaacc ttttaaagag
     3601 gtgaaacaag cttcagtagt accaatgttg ccaaaaacag gatcagaaga tactgtatta
     3661 tggatgatca ttggtgtgct atttgtttta gcagcaggtc ttacatttta cgagttacgc
     3721 aaagtcaaag actaagtagg acaaacaagc ggttagatgc ctaatcgctt gtttggtttt
     3781 aaaaaagtta ttgcataagt aacctttgaa aagataagaa atgatctttg ttttatgaag
     3841 aaaaaagaaa ggaaaaagag aatgaagaag ctatttttcg catgtttatt tagttccgta
     3901 gttcttgctg gtggaataaa cgtttttgca gatgaaatag gtattacacc agttacgcca
     3961 cctgtgatag aagaaacaac acctgttgtt ccaattccag atatttctaa aatagatgag
     4021 tctgttccgg atcaaccaac tcctgttgat ccggatatat cttcagaagt aactgaacca
     4081 acaacacctg ttgttcctga tataacggaa aattcagaaa atgaaacacc agttgtatcg
     4141 ccttctactg gagaaacaac gaatgataaa gatactacac caactaccag tgagtcttat
     4201 tcgggaacta gttcggtaga taatgattca aaaatagacg attttagttc ttcatctact
     4261 acaatagata caccaaaaga aacagaaaag aaaaatgatt taggagctcc caaaaattta
     4321 acacctgaaa atcatataaa gactgtatcg gaaaaggaaa tgactgtttc agtcagttca
     4381 gatggaaaaa ttgtacaagg aacgggtacc gaaaaatcaa ttcctattgt gacaagtaat
     4441 ttagaagaaa ttaatcatgt cccaacacct gaaactcctt tacaagtagc gattggtcaa
     4501 gcgattggtg gggtacaaga tggtgtacca cttgtacaag ataatcaagg gaatttggta
     4561 gaagatttat ccattcctgt aaaaaaacta cctagcggca atattgaaat caaaacaatc
     4621 gatgggaaaa caaaagtatt gccaaaaaca ggtgaagaac ttcaaattag tttaactatc
     4681 cttggttctc ttttaacagc agtaaccggg ggcatttttt atggtaaaaa acaatggcgt
     4741 catttgcgtc atatgtttaa gaaaactatt tagtaaggag agagtaaaaa tgggtttatc
     4801 ttatgttgca attagtcagg aagaagtatt gggaaagtgt tttaatcaaa aacacgttgg
     4861 ctcgacaggt gaacgagatc gtcgaggaaa tatgaagaaa gtagagtggg agatttttca
     4921 gatgaactct gaaaagatgg gagaaattga agtacgtgta gctgtcggtg gtgcgcctaa
     4981 agccatgcga tacaatgcag aagtgttact ggcaaatcct agaatttctc gtcgagcaga
     5041 tgtacgaaat attggaaatg acggtgaaac aaacaaaata gcacgtaata ggttttattt
     5101 gttagtcgat cgtgtggaag taaaaggggg aaatgactaa tggcactaga atttgatcaa
     5161 ggaattgttt caggattggt aaaagaaaca ttaggagaaa tctattttgt taaggcacaa
     5221 cgtccacgta tgttgtatga agataatgaa aatacaggtg agattaagga acgtccagtg
     5281 gagttttcaa gtgctgctca acaaacaacg tttgtggtaa attttccacc agaaacgaat
     5341 ttggatgaat taaattttgg tgatgtggtg gaattagaag aggttgaatt tcgattttgg
     5401 atttctattg atcgcgaatc aatgagtaac cgagcagaga atgatctgaa agtatctgcg
     5461 cttcgttata aaaaaacggg aaaaaacgtt tttcaaacgt cttcctctac tcgtgcaaca
     5521 tatgtaaaag aaaaacaaga ctcaaataaa gaaagtgcaa agaaagagta atggtttcca
     5581 ttgctctttc ttattttaaa gctagaagaa gtagctgatg tggagaaaat tgaattggct
     5641 ggctatatta ttgggataaa tttttgaatt tttaagaatt tagatagtgg ttagatacga
     5701 ttgattcttt gtgtattttg tattatttaa gtattgtgtg gtatttacat taagttaata
     5761 ttgatgttgt tagtgttaat gaaggttttc ttttatccaa tacattagtc actctatgat
     5821 gcgaggagat gagaataagt gtttcaatat aaaggtattc gtattcgaaa tcgacataaa
     5881 tatgcttcag aacgatttat tttttatctt atgttgccgg ttctgttgct aatgagtgga
     5941 tttttttctc tcttttatac aacgatcaca tgggaatggt gctgtattta ttggataatc
     6001 gcatttggtt tagctggtct atttgcgtat atgtgtttgt atttttactt ttataatgtt
     6061 tatcagcgat tggttcataa aggaaaatta gcaaagatga tacatagtcg tcgattgatt
     6121 cttgaaagag tattgaaaaa tggacggcgg aaagtggtgt attatccaaa aattttttat
     6181 cactataaaa atggcttaat tcatgttcga ttgccattag atttaggtcc ctttcaagat
     6241 cggtttaatg agatggctgg gcaactagaa gaagcctttt tttgtgattt agtagaagag
     6301 caacgagaga aaaactatat ttgttatacc tttttatacg atgtggaaaa aaatcgtatt
     6361 gggattcgag atataaccgt aaaagatggc ataatcacct taatgaagaa tgttcaatgg
     6421 gcatttaata aagcaccaca tgccataatt ataggaagta ctggcggtgg gaaaacgtat
     6481 tttataatgt ctttgatcta tgcattatta ccgtatggta cgtttgatat ttgtgatcca
     6541 aaacgtgcag atttggctag tttagaaaaa ataccaattt ttaaagatca agttttttat
     6601 ggtggtggta ttttatcttg tttaataaat ctagctaaag aaactgctaa tcgatacgat
     6661 tttttaagag aacagcccga gtatgaatta gggtacgatt atacttatta tcatctaact
     6721 cctcattttt tggtgattga tgaatgggca gcatattatg catcgttaga tactaaagaa
     6781 aaaaagaagg caatgggatg tttgaaccaa attgctttaa tgggtcgtca atgtggcgtg
     6841 tttttaattt taggtaccca acgtccagat acaaagtatt tggacggcgc gatacgagat
     6901 aattttggtt tacgtgtaac actgggaaga gtatccaaag ctgggtataa gatgatcttt
     6961 gaacatacag taaaatcatt atttaataag ccgattaaag gtcgtggtta tattgattcg
     7021 ggaaccgatc aagtacgaga attttatgcg ccatttatcg atataacaag atttaacttt
     7081 tttgcggaat ttaaacaact atctgatcaa ttaatggctg aaaaaatcaa ggagacgaaa
     7141 gagggaaaaa atgtatgaag aaatcccagc tacatgtgga actcgattag gaataaaaga
     7201 tttaatgatt gactcttttt tggcaattgt tttcagtgag aagagattaa gcttctttat
     7261 caataaaaaa gtagagaatc gttggagatt agaacgtatg tatccaacga gttaaccgat
     7321 tggttacagt tgccttatga caattatcga aggcgaatat gacgggtttt tacttcaaat
     7381 aaaagtgctc gatcctatag tgaccaaatt gtcgaaaaag cagcggaaca gcctgttttt
     7441 tatggacgat tcaaaggatt ttcctgttct tatgttaaaa gtaaagaccg gtctcgactg
     7501 tacctacaag tagaggatat taacttgtcc tttgcgttgt cagaaaatta aatttgtcct
     7561 cattagaata cggaagatgc tgtgcgtgtg ctccacaagc aaacaagcct cagggcggaa
     7621 ggacgcggaa gcgtcctagc gttagcgaaa gccctgaggt gcagggtgcg ttgtggggcg
     7681 tgcgcgtagc gcacgcctaa aacccccata ttctaacagg ggggtagaat ttatattcgg
     7741 tggcaaagat gggtgattga aacattgata taacactatt ttgcttcaaa cattttgtga
     7801 tatttttctt cgctttgtaa cataaaatat cacaaaagaa aggaaagata tatttggatg
     7861 ggacacaatt aaagcaaatg cggttaagtc ggaatttgac gcagaaatta gtggcagata
     7921 cattaggatg gccacaatct tttttatcaa tgatagaaaa tgaccaacga agttgctcga
     7981 aagaacaaga agaagcactt tgtgaatttt atgcgaattt ttcaagtttt acgagtttag
     8041 aaattaaatt tgattatgtc cgtgtacgag ttccgacaaa tgatacgaaa acagttgtgg
     8101 aagaaatttt gggaatggat tttaactctt ttttttatgc agaaacccgc ttatatggtt
     8161 atattgaaat gtatcaaaaa ggcatgatcc gtgtattaaa ttctaaaaaa ggtgacgata
     8221 aaggagtgtt gatcgaatta tctggtcaag gctgtcggaa ttatgaaagt gtactggatg
     8281 aacgaaatga tagttggtat caattcttta cacgatgtgt ctctttcaat ggtgtgcctc
     8341 gaagaattga tattgcggta gatgattttg aagaactatt ttctttacat gaatttgcac
     8401 aaaaactaaa tcgtggcgaa tttgattcaa aatttcgaac ctggcggttt caagaagaaa
     8461 aacagttgtt agataatcta agtagtggct tatctgttta tttaggtagt tcacaaagtt
     8521 tgatgtattt ttgtttttat cagaagaatt atgaaattgc aaaaaagcag aaacttccgt
     8581 tggaagaggt cgaggtaaaa aatcgttatg aaattcgctt acgtgatgca aaagcggtca
     8641 gttttattga acaatactta aataaagtag atttttcaga tttaacgatg gggattttag
     8701 ccaatcagtt aataatgttt gagggagaga aaaacgcaaa tgtgtggaaa ccatggggaa
     8761 aattgattca gaacgttgga aaagtcaatt tgacaataac accagaaaag ccaaattata
     8821 atcggaaatt gatgtattta aagacacaac ctgctcgtac aatgaagatt gtaaaaactg
     8881 tggatgaaat tttaggattg aatcgatttg gagaaatttt agaagaaaca gaattacggg
     8941 aacaagatga aaaaattgta gaagatgagg tgcgtgctgt atatgaatat atacaagaaa
     9001 atccagacgc gattcattat ttgctataaa gcttgcgata agatgggtga aacatgatta
     9061 aaacagaaga ccaatgatgg ggagagtata tgattgcttg ttaacaatta ataaaagaaa
     9121 cgatcctatg tgttgtaaat gatctacagt tatttaaaga atatttagat tatcaacaat
     9181 caatcaaaat gttacatgta gaaatagggg aagtattaag ctaccgtatt agtgattcgt
     9241 taaaatattc gttgacaaag atttatttat atgcaacaca agtatgtgga tatacgaaaa
     9301 agcagtaaaa caatgatgaa atatgacgat ttgccaagca agatatccta gatttacaac
     9361 aagtattgga agaaaaactg tatctatata tgaaaaatag aggggaaaag aaatgattcg
     9421 atttttattt tctgtaggtt ggcatttttt gaaattactg tttcttttag tttgtattgt
     9481 ctgtagtatt atttggttat taacacttta aagaaaggaa tatgttggaa aaatgggaca
     9541 gtattttctt accggtgttt ggtatgtatt gaaattagtg attattttag gtattgccta
     9601 tatttgtttt agaattttat tatcgggaga aggaatgcca acgccaaaag attttatcat
     9661 aagcgtgttg aattttgtgg cggatcaaat acaagtgata attcattggg cgcagcaacg
     9721 ttttggataa aaaaatatat ttaaaaaatt taatgtttag aagtgggcta tgtgatagaa
     9781 aatttcgagt ataagttatc tagattgcca aagaaaaagg gagctacaca aaaagagctg
     9841 gctaataaat taggtgtcaa tgtacaaagc gtttctaatg ttgaggaaga taagagctaa
     9901 ctacatttag taatttagaa aaaacagttt ctttctttga tgcttcatcg attgaattat
     9961 ttggtacgaa tagggatcga ataattgcag atgttccatt aatattagaa gatatggata
    10021 atttgtttga aaaaattcga atgatcttta tgactgaaaa agtaatcaaa caattaaaaa
    10081 aaagtttctg tttttgatgg aaaaaaacta tcaaaaagaa tcaattattg atctttttga
    10141 tagcctttat ttcgttgatg gaatccattt attagctgtt tcaacaatcg ttgttgtaaa
    10201 aaagaattgt tttggatgcg taattctgga tagccttcaa tggcttgatt ttgcttttca
    10261 aatgcttgtt ttgcatgata tgcttcacga tctaaaataa atagataatc tgtttgaaat
    10321 gcttcaaatg tacagatttt ttgagttttt aacaaatgaa gataggtatt gacaaagtgt
    10381 tctacacaca taaagttttt tgtgtgtgag gcaaaaagat attctttagc taaaaagtta
    10441 gtagtaagtg tatcagataa ataaaagtaa tctggtgcgc gatcatacat ttcgacaaaa
    10501 tagtcataaa gtaaagcaat tttttgtttc tctgctagaa gatactctat ttctttggaa
    10561 taaaactcat caatttgatg agtgtgttta ataacttgaa caaataattt catatagtgt
    10621 tgaatgaatt ctgttcgttc gattggttca ccaaagggtt gaagtatggc catcattctt
    10681 tctcctttca ctcaagggaa aaatcccttg agaatgaata catgtattta gtaaaaaaat
    10741 attttgactg cttcgatggt tttttttcca gtttgttttg cgataagttt aggtaaaatc
    10801 tgttgtaacc attgttggtc agatacgtga tcgaagaagt agccgattgt tgttaaaatg
    10861 agtgtatctg ttgtatgttc aactaaacgt acagattgaa aaggacttaa cccagcaaca
    10921 tagttcaaaa tattttcatc tgtttgttca aaagatccta aatagttatt ggtattccga
    10981 tcgtaagaat caacgatagg aatagcattt gttcttagca ttgcaatctc tccttgatta
    11041 gaaatactca gttatcattt tttattgata gatttagaag caagaactct taatttatgc
    11101 gttgttggta aattgtcaga ccgttcttca cctaagattt catatagagt tattggtttc
    11161 attgttcttg ctccttttat ttttgttatt tttaacattg actaagaggg aaattccctc
    11221 ttagtaataa ctgctttcat aaaccggttg attatcatag cggtaaaatg tatcccaact
    11281 gcgattaccg tataattcat tgggatataa ggctggatta attcgttttc taacaatgct
    11341 gtctttggtt cgtgcaagcg gataatcgat aaattcattt ggtattggca tagtttctcc
    11401 actcatagaa gtataggttt ttacagtttg ctttttgatg ggtcgaaacc atgctgtttt
    11461 ttcggttaat ttaataattt gccaaaaatt aacgttggtt tgttcatagc cccaactact
    11521 ataaaaaatg tctcctactt tgtatggtgt atttgctttc atgatctatt cctccaataa
    11581 tgtttgattt gccttatttg atacttagcc gaatcaacca accattttcg gcagcaagtt
    11641 gtctccgagg tgggcgacct tttgggagat ttcaatttct ttttgtttct attcacctca
    11701 ctttttgttt ttggttggtc attttcgtat agctaagcgt tcgtcggcgt gctttgggtt
    11761 cacaacgtgt ggtaagagaa aacaagggaa tgctgtagga aaaagttttt tatgaaatga
    11821 tcaaaaaagt ttttccgccc cttgttttgt cttaccatcc gttgtaaaag caccgtcgcc
    11881 gatgatcgct tagattgggt atatgaaagt gaccaacaaa aaaagtatgg gaataaaaag
    11941 aaatcgcatt gaaatttctc aaaaggtcaa gatggtttta ataaaaactt tcttttaata
    12001 catttgttat tttgataatg tggtgatata aaatagatca aatagttaaa tgaggtgttt
    12061 atatgaagaa aaaagttatt ccttattgtc caaaacctaa aaatgctttt gaaagaggtt
    12121 tgtttaaata ttttgaaggc aaaaaaggac aagaccataa agaggagaat caaaaaaaga
    12181 agtctaagaa aaaaagtgag gagttttttt agaaagaata aaactggaac gtaaagattt
    12241 acaagtaatc agtaacccgt accttttatt tttaaagagg tcgtttttct gtaaatcgtt
    12301 gtaaagaatg actaagttgt attatttttt cacaaagatt tatattataa tgtttctttt
    12361 taataaaaag cttgttttta agctaattat tatatgtgtt ttattgaact aactcttgac
    12421 agatcatcaa aaagaattta tagttgttac cgtagtagct actaaaaata aagtgaggat
    12481 taatcaatgg aaacgattat tcaaatcatg aaaaaatatg atttttcaga gatggaaaca
    12541 agagtgtata ccacactatt agaaaaaggt aatcttacag gatatgaagt aagtaaaatc
    12601 tcaggtgtgc cacgctcgaa agtatataac attttggaaa aactattaaa aaagaatttg
    12661 attgtcgtta ataaatcaga acctaaactt tatcatgcca tctcggcaaa tgagttttta
    12721 gagaaattag aaaaaagtgt taaaaatgat ctttcttttc ttaccaagaa tctagggatg
    12781 atcaaagaaa aagatgaaga agaaatgctg tggaaagttg atggcatcga gtatgttctg
    12841 gataaagtag agcatttggt gaaaaacgca aaagaaagtt tgctcattca agtttggcat
    12901 gagaacctca cggattcact gctaaagaca ttgcagcaag ctgaaaaaag agttgataag
    12961 tttgtactaa ttttattcag ttctacccat gaatatgatc ttcccttaga aaaatattat
    13021 attcatggct ttgaaacaga caaacttgct gactttggag caagatggat aaacatcgtc
    13081 gccgatgagc aagaagtcgt gtttggcacc attaacgaag aactgcaatc aaccgatgtc
    13141 acttggacaa aaaaccacgc catggtcaat ctagcaaaag aatatgtcaa acatgatgca
    13201 tatacactaa aagtgattgc agaatcttct gaagaactta aagcaaaata tggtgaggac
    13261 tttgagggaa tcagaaagat ttactagtag gaggaaattt ttatgattcg gaatattttg
    13321 ctgacactaa ttgtcgcaat caatctatat tttattattt actttctaag agaccttttc
    13381 aaaaacaaac aaaatttcaa agaagaacct ggggatatgc gtctgttgcc cttcacgtca
    13441 tttatcatct tctttctttc aacatttggc gtttccgact ttgctatcgg aaccgttctt
    13501 tatccaaagt tgaaatgggt cagcatgaaa aaactacccg gaacgttgaa tacccaatgt
    13561 gtcgtaccgg tagccgtcat ggcattgtct tatatcactg ccatcaacgt agggatcaag
    13621 acactagctg tttgtattat ctgtcaaatt atcggtgcat atttaggacc aagatttgtc
    13681 gtgaagttac cagagaaaac gatcaaagtg tttgtcggca taggattggt cattgctgca
    13741 ttattgattt ttgcaggaca aatgaactgg attccatcaa acggaacagc cagtgaattg
    13801 tatggcggaa aattaatctt ggcaggattt ttactctttg tctatggcgc attgaacaat
    13861 atcggcattg gttcttatgc gttaacgatg gtaactgtct acttgctagg cttgaatcca
    13921 gtggcagcct ttcctatcat gatgggtgct tgtacattct cagtaccgat cggtagtgtt
    13981 caattcatta aatttaatga atacagcaga aaaatcacac ttttcacttc aaccttcggt
    14041 gtattggggg tattggtcgc tgtattccta gtcaaatctt tagataccta tgtcttgaaa
    14101 tggatcgtaa ttttggtctt actatacagt gcatatacaa tgttgggcag tcaattaaaa
    14161 aaagtttcag cagctaatta aggagcgatt caaatgttat ttcttaaaaa agaagatatt
    14221 atcaaaagtt tctcaatgag agaagccatc gatgcagaca aaaaagcatt gtcattgtat
    14281 tcagcaggaa aggcaagtgt gcctcttcgc accaatatcg atgtgccaaa aagcaacgga
    14341 caaagcttat atatgccagc ttatgtagaa ggtggcgaag gtgcgttagg tgtcaaaatc
    14401 gtttccgtgt atcctgaaaa catcaagaaa aacttaccaa gtgtaccagc aacgatgatc
    14461 gtccttgatc cagaaacggg tatggtatcc gcttgtttgg atggtaccta tttgacacaa
    14521 ttaagaaccg gcgctgttca aggtgcagca acagaacttc ttgcaagaga agatgctaaa
    14581 atcggcgcac tgatcggaac cggcggacaa gcacagtcgc aattagaagc gatgttgacc
    14641 gttcgtaaat tagaagaagt tcggattttt gatattgatt ttgaccgtgc cagccgattt
    14701 gctgaagaaa tgatgcaaca gttttctgta acgatgcgcc caacaaaaac cagtcaagaa
    14761 tgcgttgaag gagcagatat cattacaagt gtgaccactt ccaaacgtgc gacattctct
    14821 gctgagtggg tcaaaaaagg tgcgcacatc aacggagtcg gtgcttacac accggaaatg
    14881 tgtgaaattc cacgagaaat aatcaaagcc gcagatgttg ttatttttga taccatggac
    14941 ggtgttctaa aagaagctgg ggacttcatt tcaccgctgc aagatgggta tattcaaaga
    15001 gacagttatc atggggaact aggccaactg atcaatgaag aattgatcgg tcggacaagt
    15061 ggagaacaaa tcacaatctt caaaacagtt ggttcggctg tattagatgt ggtagttgcg
    15121 acagaaatcg ttaataaagc taaagaaaat aatttgggaa aattgattta ttaaataaaa
    15181 agaccggaag taaaccttta attagtacat ttataaaaag agaacaaaaa agactgtaat
    15241 caaaatgcga ttacagcctt tttttgtttt atttgggagg agatagatcg tgtttatgac
    15301 tgtatcagat catttaaagg tacttggttt tatcccttta tctagtagag ggaagtatca
    15361 attgtatttg ttagatttac caccctagct tttcactggt gattgaagga acatacgata
    15421 aaaaaagtgg taagtatttt tatgatttct attctatgaa acttgatcct aaaacaaaga
    15481 aaagtacgca gcaccggatt tattgtacgc atgttcctgt aaaagtaatg tatcgtcgtg
    15541 tgagaaatta tttatacttc ttacgtaaaa aacagcaatc aaggaacgga aaaaatgcat
    15601 aatgaggaat atcaagtgaa atttttatca gttttgctct gtggtattgt gtatcgtcat
    15661 tagttcaatg agtattaaca gggaggaaaa agcaaatgaa tttttttcaa aatgtgttta
    15721 ctgattttgt tggggatttt aagtatttga ttatgttggc gatgcttggt ggtgcggcat
    15781 atattgcctt tgaacggaaa gtaagcaaag ccattccatt gattcttgtg ctagcagtag
    15841 ctgtttggtt agttggagat acgtctggcg tattcaattg gattttggat cgtatgcggt
    15901 catgggggcg ataaaatgaa caaaatacgc aattggacaa agatttggta tatagagcga
    15961 gtgttttata aattgtggga tggggtcgcg ttacctcgtc ccgtaacaca aacatttgcg
    16021 atatggtttg ggattggctt ttttgggggg atttttcgaa tttttccatt tgttatggaa
    16081 catacgatta ctcgacaatt tttttgtccg tggttatttg cttttttaat gacacgaaaa
    16141 caatttcaag gaaggaagcc ttataattat ttggcttctt ttcttttatt tcagtgtcgt
    16201 tcaaaagaat cctttcatgg ccaatgggta aagccaatag aaaaaaaaga aatcaaacga
    16261 ggaattcgtt gtgtagtacg ttatggaggt gaaaaggatg cgactaccaa aactagtgcc
    16321 taagattcct ttttattatt tagaagacaa tttagttttt ggagaagaag atcgtgtgta
    16381 cgcttattat gaattcccaa tgtattcata tagcttcgtg ggagaagatc aggcgtatca
    16441 actccaacaa gcaatcatgc gtctgattcg tcaaagtcat tcttcgcatg ttcggttctt
    16501 tttttgtaca aacgaagaaa aaattgaaaa aacgattaaa gcctcaaaag aattggtgcg
    16561 ttctgaaggg gaattaaaaa cgattgccta ccaacattta gaaggtgttc agcacatttt
    16621 agaagagcta catggaaaat atattcaaaa tgtttgttgg tatgttggga ttgagctggt
    16681 attagaagaa agtgctccca aagaagctca ttggatgaaa gaatttcgta ttgcgacaaa
    16741 agattttttt caccgatcca atgcccgttt gtttggagac tttgaaacca tcccaaatga
    16801 aaaaattcaa cgatataagc gattggagaa tttattgtat aaccggatta gtaaatactt
    16861 tcggttacga aaaacaacac cagaagacat ggtgttttta gtggaacatt tagaaggaaa
    16921 aaaagaacaa tccttagatg aagtgatgta tcgacccaat agctttaaag aagaaacgaa
    16981 tacgtatacg tatcactatg atgtattgaa tttggccagt gcaggaatca cagaaaagaa
    17041 aaattatcta attttagaac aagggacaga aaaagaatat atcacgtatt tatctctttc
    17101 acgtatgacc cgtgatttgg tatttccatt tcaatcagag atttattatt ttttacaatc
    17161 ggaattggat tttccaatcg attcttggtt agatgtgaat cttttagaca atcgtttggc
    17221 cttggcgaaa gtacgtggca aaaagagtga tttatctgat atttatgaaa gtggtatgtc
    17281 ggttggaaaa gatgtaggaa acaatgtacg cgaagcgtac caatcagcca atgagttgga
    17341 acaaaaatta gaagatacgc gagaagattt gtatgtatta tcactaaacg ttcgcgtgtc
    17401 agggaagacc tatgatgaaa tgttaaaacg tgtgagtgat gttcgcaatt tttttgatgt
    17461 ctattctatg tatttagaag taccgtgcaa tgtacagctg tttttacatt ttggaagtta
    17521 tccagcagga aggcgagtga ttgaggatta tcgtcaatat gtaggcgcag aatttattgc
    17581 tagtcttggc attggtgcga cacaacaact aggagatggc tgtggtattc cgattggatt
    17641 taatgcagaa acaggaatcc cagtgtttat tcaaccatgg ttagcggcac aaggaataaa
    17701 aggaacggtt accaatactc ttgccaaagc attaattggt tcgctaggtg gtgggaaatc
    17761 cttaactgaa aatctcttgt tattttggtc ggtgttgttt ggcgccttgg ctttttgtat
    17821 cgatccaaaa tcggaacgtt caaattggtc cgaagatttg ccatattttg ccccctactt
    17881 aaaaacagtt aatattacca gtgagaaaga aaatattggt ttacttgatc catttcaatt
    17941 gatgagaaga aaaaaagatc aagaaagttt agccttggat gtgctcacct atatcacggg
    18001 tgtaagtatt cgggacgaag accggtttcc tttgttacaa cgggcagtaa aaactgtttc
    18061 tgattttgag ggaacaaaag gattacttct agtgattcag gaattatatg cacaagaagc
    18121 agcgatcgca caacgcctgg caaagcatat tgaaagcttc aaagaactat ctattgcagg
    18181 cctattattt ggagatggca cgaatcaaca agggttaaat ttggatgcca aatgtaatct
    18241 cgcactggtt caggatttaa cgttaccaga tagtgaaact gcaccagagg actataactc
    18301 ttctgaaatt ttaagtatag caattatgat gagtttagct acatatgcgc tagattttat
    18361 taaacaaaat cgagatattt ataaagcggt tggcttagat gaaagttggg cgtggttgaa
    18421 tgttgcgcaa ggaaaaattt taggaaataa attggttcgt gaaggaagat ctcgcaatgc
    18481 agggattgat ttttcaacac aaaacacaga tgatttaggc gatgaaaaga tgagaagtaa
    18541 aattgggttg aaattcattt ttcgttcgaa tgatcgcgaa gaaattacca aagcattaaa
    18601 cttttgtgga atctctgtca cagaagaaaa tatccagcgt gtaaaaaatc tagaaaatgg
    18661 agaatgtctg ttttgtgaca tccatggtaa tattggcatt ttgtatgtgt attattggtt
    18721 tgaagattta ttccatgcat ttgatacacg accgccaatg gaaaaaaact acgaagagga
    18781 agaatataaa gacgagatat tagaaaaact ggttgaagaa attgaggtga attagatgaa
    18841 acagtggtat tcaaaggttt cattttcaat tactcgccga aaactggttt tcataagtct
    18901 agtcataatg atgggtgttt ttttctttca cccaatattc gtagaagcag ctggttggat
    18961 aaaagaaacc ctaggcggca aaaatgccgt atatgatttt gaaacatatc ctttagataa
    19021 ttataattta gatttttttg tggatacgtc gtggaattgg ttgccgtgga aatggggtga
    19081 aggaatttct aatacagtta tttatgggat atttgccgtt accaatattt tatggatgtt
    19141 aaatgtctat ctgtgttact ttttagggtt tcttgtccaa gaagcctttg aattagattt
    19201 tctttcagag ttgattggtt cgttaagtca ttcaattcag aatattgctg gaatgaaccg
    19261 tgcagggaca ttgagtcatg gattagtccc tttctttggt ggattgttaa ttgctttagc
    19321 aggttgttca gtagtttatc aagggatagt gaaacaacac cctacgcaag cgattcaaca
    19381 agcagtgaca tttttattta cgtttatctg ttgcggcatt ttctttatga atacgcacac
    19441 atatttagag aaagccaatg aggtacaaaa agaaatcaat agtgaaatgt taaccattgc
    19501 caaagatctg ttacctgaac aaaaaggaaa accagatgca attaaagcca ttcgtgaaaa
    19561 tctatttgcc attcaaatca agcaaccctg gttagtattg caatttgggg atagcgatat
    19621 aaaaacagtt ggtgaagaac gtgtaatcaa tttattaaaa gaatctcctt atgatgaagg
    19681 agagcagcga aataagcttg ttgaagaaga agtaaccaaa caaaaaaatg gcaatctttc
    19741 ggtacaaaaa gtgtatttgc gttttggtat ggtgttttta acgctcattg tcaacattgt
    19801 cattagtatg ggtgtattgg cattagtcgt catcttaatt gcgtcgcaag tattgttctt
    19861 gttatttata ggatttttac ctgtagcgat gattttctcg ttgtttccaa attcaggatc
    19921 gttattaggt aatgccttac agaaagtctt tcaattattg tttacaaaaa tgggcatttt
    19981 actcatttta accattactt tttctattag tcacgagtta aaccacttat ccaagaacaa
    20041 aggaatgatt tggaccagtt tcttacaggt gacgttatgg ttttcagtct cgacacgtgt
    20101 caatgaatta ttaggttgga tgaaaattgg tggcgcacaa acacgagctg gggatcgaac
    20161 gggacgtttt ttacgtggca tgatgttagg tacaatgggt caaaaaatga tacgaggaat
    20221 tggtgaaaat ttcgttggtg gtgctgccgg aggtgtagct gaccaaatga tttccaaatc
    20281 tatgagtggt caacgaagta taactactac acctcttata gagcgagtag gaaaaaaagt
    20341 ttcagctatg acagaattac cggcaatgat tgaaagtagg gcagaacaat ggaaacggac
    20401 aattcagtat gtcccaacca atgcccaata caaagcacaa aaaatgaaat acgattattt
    20461 ctctgggcga atgaatgagt cggaacgaca aacacgagcg cgtaaccttg cgaatgagcg
    20521 acagaagaat ttttatgaag cacgaaaaga tgcattgcaa ttagagcaac aagagagaaa
    20581 acaacaagcg atgcgttcaa tggcgcatca aacaggaaaa acacaagtga cttctggtaa
    20641 attacaaaca cggaaaagtg caatgattgg tcgaacagaa caatttccta cgatagaaaa
    20701 aagaagtaac atttcaaatg tagctaaaaa taagaaattg tcgaagcagg aacatccgat
    20761 tcatgaacga agtacgaatc gaaagattga aactccggtt cgttggactt cgacttcttg
    20821 tcaaaacgcg agaaaaggta gaactaaaaa agaataagga ggagggctat gtttcctcgg
    20881 aagaaaaaac tgtctattct ctctgaacaa gaaacaagac taaaaggaaa gaaagacaag
    20941 aaaaaagaaa gctataaggg agcgcgaaaa aggcgcttcc ttttttttgt aggctggagc
    21001 tttttggttt ttagtgtttg ttttggagtt tataataatt taacttctca agatacgcat
    21061 acaatccatg aaacaaaagt cattgataaa caattaaaag atatgactgg aatagaaagt
    21121 tatgtgaaaa attttgcatt tttgtatttt acagtgagtc ttgaaagcga ccaacaaata
    21181 aatcgatcaa aagcagtaga aagttattta gctgatgggc tgcaaatgga taatgaaata
    21241 gataaagaca tagaaacgaa tgtaaaagtt aaaaatgtct cgatttgggc aatagaggaa
    21301 catgaatcaa aaaaacatac atttgtggtt atatttcatg tcttattaga gatagaaaaa
    21361 gaattcaaag agcgtgtgta taaggtggag gtatatcgaa acgaattatc atatgccata
    21421 gtgaaattgc ctattgtagc gagtaatgtt aaaaaagcaa cgattaaagt agacactcgg
    21481 ttcgaaacaa cgagcatttc tcgaaaagaa caggaagaat tagagaaatt cttatatacc
    21541 ttctttcgta tttatcctaa agcaaacgca gaagaattaa aatattatgg gacaactctt
    21601 ttaccaattc aacagccttt agcctttgaa gaactatcga atttgcaagt cgcaaaaaaa
    21661 gagggtagat atgagattca ttgtaatatt ttgtatcaag atcaagaaac gcacctttgg
    21721 tttaccatgt actatgaaat gtctatgaaa caattagaga atgggaactt tcgaattgag
    21781 gcgattcact agtgaaaaag atttttttat ttagtggcat ctgtatgatg agttgcctgt
    21841 ttttattatt tgcgataatt agcatgtcta gtggtgaatc gaagcacatg gattcagcag
    21901 ttggtgttac tcatacggga gaatatatcg aaaccttacc tattttttca gagatcaaag
    21961 ggcgtggtcc gataacagat gaacatgccc aagcagcagt tggtgcagct gtaaagtatc
    22021 agttattacc gagtgtgatt ctttctcaat taggatatga atcagcttat gggcagtcac
    22081 aatctggaag aaacgatacg aacttttttg ggattacgtg gtttcctggt tgtccctatc
    22141 cacaaggttc tgcacgtggg attggtggct ctgaaggtgg tttctatatg aagtttccga
    22201 acctgaatgc ttgttatgac tattatggat ttatggtggc gagtcaaagc aactttaatc
    22261 aatgtgtggg caataaagat cctggaagtt gtttattgat tctaggtcgt ggaggttatg
    22321 cggcggcagg tatcaccgaa ggcagtccgt attatcaagg cgcaatgagt atcataaagt
    22381 cctataactt aacggaatat gatgcctttg cgatcaagca ttggaatagc ctaccaacca
    22441 aaaaaacttc gcttattgca ggaaaaagta aaggcaatgc gaaggttttg gatcggatca
    22501 ttggacgacc cgtaaataat ggggaatgtt atggtggtac ggcatggtat gtatctcaac
    22561 taggtggccc acagttaatg ggttctggac acatgtttgc gatgttaata ggggaagatt
    22621 ataactgggc aacctatggt tggcaagtga tcatgaatcc aaagcccagt gacttacaag
    22681 caggagatgt ggtcaattgg caagcaggtg gtgcattgtc tcctggaatc tatggccaca
    22741 cgggtatcat aacagatgtc tcaaatagtg gacaaagttt ctccaccgta gaacagaacg
    22801 ccgagcaggg gcgaattatg gcacggtatc aacgcagcta taaccaagcg aaaattcgaa
    22861 gtctggttcg gaaagtgaaa taaaagaatt ctttcgatga aaatttttag tataaaaggg
    22921 agaaggatcg attgtgaaaa attattattt tttctttcta agttgttttt gtttgtttgg
    22981 acttactagt tgtgggtgga ttttttctga agaaaaaaca gaagtttcct taatatcaaa
    23041 agaaaagatg atacaagcag aaagttcaag taaagaaagc aaaagaaatc aggaagacaa
    23101 aacagaatgc aatatttctg aagaaatcca atatttgtta aagaattttg gaagaaaatg
    23161 ggtgaattac acggatatat acgaacgaaa tcaatctgta aaaaactatt taaccgatcg
    23221 ttgtataaaa gaaaatggga ttgacaacga tccacatgta caatataaaa caattggccg
    23281 tattcatact atcacacaag atgtaatcca atcggatcaa tatttattat ttggagaaga
    23341 aacaacaaat gagatgactc ggtttgtgtt acttcaaatc aaggtggatc aagaagtgca
    23401 gaagattgat aaaatcaaga tttattatgt aagaagtgca tattaaaaga aaaatgacgt
    23461 tgttttattt actagaaatt atagtttaac gaaaaggaag aagctgaact atttaagatg
    23521 tttaaagaat atgaagaatt ttgggaataa atcaattaaa aggaaagaga taagaaaata
    23581 atgtacaaaa aataggagga ggaatgagat agaaacggaa aaagtagaag tactttggtt
    23641 aaaacaagta ctagagttga aaaatgtctt tttgaaccat gatttagtga atcatgggat
    23701 gaatttgtga aaaaatgatc ctaagtagat acacggacgc aaatttctat cgctacatac
    23761 aaaagcggat gagcttgtag agcaaattta taatcagtta gatgtaattt cagaacaatt
    23821 gattatgatc gatgggttcc tgtataaact ctataggaaa cacagttgaa ttggataaat
    23881 agattcagaa tttcaaagca cctggttatc ttgattattc taatatacat ttaaaggggc
    23941 aactccttcc cctgagctac ccctagagag tgatatagga atattcccaa gtctattata
    24001 agaataccta cacgttatct caacaaattt gctcttacac tcatagcttt taccttataa
    24061 aattaacgtt cattaccatt aaaactttca gaaaaataat ttatactttt attttttatc
    24121 gaaaaaaatt ctaaatagag tatttttcat ttccgaacaa attatgatcc tttcttgaca
    24181 aatatagaag ctacaatgtt tactagtttt gaataagaaa gatagtgctg tgtcttttat
    24241 aattgattgt ttgatttttc atgaaaaaat gctcgagtta aaaaaacaaa ttgaacgttc
    24301 attttatttg acttagtagc tccttcttgt taacattgtt tcataaacca tttaatttta
    24361 taaattaagt atgtctttat ctttctgaaa tgtgagatgc tactttaaaa ttttagggat
    24421 attaattgat taaatttatt gtattaattt acctaaataa gtcagacaac tgaaaggagc
    24481 atagattata gaaatcaaag caccacaacc tttggagatt ttttaataga tatatatttt
    24541 atcgtaaatt ttcaaggaat gataaagtaa gcgaatttgt tcaaaacgta gataaagagg
    24601 tgtattatgc gaaaaagatt aaaattatta ttaatccttg ttttattttt tgtatttttt
    24661 cctttagcac atacattaat tacaatgcaa agtgttgtta gtttaccatt ttacctactt
    24721 ccatttacaa gctatccgta tattggtaat agcatgtctc aatttttgtt ttgggttagt
    24781 gtatgtttat taatactatt gtttattatt ctaattatcg tgttattcta tcctaaagcg
    24841 aataattata tagaaaaaaa gacagctagt ggaaaaatag caatccaaaa aaaagcaatt
    24901 gaaaattttg tattaattgc tgctaaagaa gaacctttta ttgttgatcc aacagtaaaa
    24961 tctgtaatta agaaaaagaa aatcaaaatc tacatacgag gtaaaatacg taaagctttt
    25021 caaacgcaag agcgccaaac agcatttata agaaaagtga atactaattt gaatgaacta
    25081 tttggtgcag aacaaacaat attaaccgaa ataattttca aagattatca tgaaaaaaat
    25141 acaaatacta aatcaaaagt tttataggag aaagcgatgg cagaattcat atcaaaatat
    25201 aaataccctt tcatttttgg atgtttggga ctattgttag gaatattgat ttttacgtta
    25261 ggatttatta aaacagtaat attaacagtg ctgactattt gtggttttta tttaggtaac
    25321 tatttagaaa ttgttggttt ttttgacaag caaaattagt taataaccgt ttttacggta
    25381 agaaataaat ttctaatttt taggaggaaa aataatggag aacaaaacaa ataatacaaa
    25441 aacagaaatc aaaaagaatg acgtggcaaa aacttccgag ataattaaag gagaactagt
    25501 ttttgaagat aaagtaattc aaaaaataat tggtattgca ttagatgaga ttgatggact
    25561 tttaacgatt gatgggggct tcttctcaaa tatagctgga aaattagtaa atacagataa
    25621 cacaacttct ggagttgatg ttgaagtagg aaaaaaacaa gtcgcagtag atctttcaat
    25681 agtggctgaa tatggcaaag atgtaactac aatttatgat aaaataaagg aagttatctc
    25741 aaaagaaatt aagaaaatga ctggcttaga tgtaattgag gttaatgtag acgtcgtaga
    25801 tgtaaaaacg aaagagcaac atgaaaacga ttcagttaca ctacaagatc atctttccga
    25861 tgcagcttct actactggag aatttgcttc aaaacaattt gaaaaatcga aagaagcttt
    25921 aggagttgca agtgaaaagg taagtgacgg tgtagagaac gtaaaagaag catctgaacc
    25981 tcgcgtagaa taacaaatct tttttattta atgtacaatc tataaaatta aaagactgta
    26041 tctattgttc ttctatatat agtgttaata tagaaaatac aatttaaaat tagggtccat
    26101 aatattttgt gttgatagaa aatcgacttc gattttctat caacactttt tcttcgtttc
    26161 aaatcaaaac aaaaagagac tcacttttgg taaaatttaa gatgttaatc attttgtgtg
    26221 tcggttaatt tttatcaatc taagatttaa ttatagatca ttaaaaatga gcggtcgttt
    26281 ttatatactg aatatggtac actaaattaa aatacctttt ttgaggagat cattatgtat
    26341 aaagcagcga acgatttatt taatcgatta aaattagaaa aaaaggaact cattttaaag
    26401 tatattttag atgagtttgg gagagtaggt tatgaagcca cttctgttcg taaaattgcc
    26461 agtaaagcag atttaagtgt aggatcatta tatcagtatt tcaacaataa agaaggtatg
    26521 ttaacagtcg cgatagaaca cgtttttgtt attttagatg aatatgttag tactataaac
    26581 gacgctcctt taaaactaaa agatcgttta gaaataatgt ttgaaatgat tttaaaatta
    26641 ggaaagcaac atcctgaact catcatattt tataataaaa ttcctagtga taaaaaaatg
    26701 ataactgaat gggttaaaca attctataat aatagaagtt tttttgacgt ttactggagt
    26761 actgttaaag aactacaaaa tgaaaaagaa attagtacta aaattgatcc agttatctat
    26821 acatttatta ttgatacaat gatgttatct ggagagctgc ttcaaaatac acaatatcaa
    26881 gaaataaaag ccaaagtttt tctagaagac tctataaaaa gttcattaat attaaaagaa
    26941 aatgttatcg agacgctaac tgtacttgca aaggggcaat aaatttatgt caacaaacaa
    27001 gaaagataat aaaaaagaag aaattatatt taccgcaatg aaacattttt ctaaatttgg
    27061 ttatgaaaaa acaaagcttt tagatattgc taatgaagct aaaactagta ctactactgt
    27121 atatagtttt tttaaaacaa aacatgattt gtatgaggct gcagtagagt atgatttaaa
    27181 tataataaac tttgaacttg aaagaattgt tagtgaaagt tccacactcg atgaatatat
    27241 tataaatata attgaaagag tacggctaaa atctgataag aagcaattat tgttaaattt
    27301 ttatatgctt gtttcaacaa attctgttca gattaaaaat ttagagatta tagaagagtt
    27361 tgaaagaaaa aaa
//
LOCUS       DAZABD010000038        27101 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000038 DAZABD010000000
VERSION     DAZABD010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27101
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.038"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(57..509)
                     /locus_tag="ACHALG_001669"
     CDS             complement(57..509)
                     /locus_tag="ACHALG_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN2587061.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(974..1342)
                     /locus_tag="ACHALG_001670"
     CDS             complement(974..1342)
                     /locus_tag="ACHALG_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HFN2587062.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            1492..1974
                     /locus_tag="ACHALG_001671"
     CDS             1492..1974
                     /locus_tag="ACHALG_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HFN2587063.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            2011..3021
                     /locus_tag="ACHALG_001672"
     CDS             2011..3021
                     /locus_tag="ACHALG_001672"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HFN2587064.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(3136..3849)
                     /locus_tag="ACHALG_001673"
     CDS             complement(3136..3849)
                     /locus_tag="ACHALG_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587065.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(4022..4629)
                     /locus_tag="ACHALG_001674"
                     /pseudo
     CDS             complement(4022..4629)
                     /locus_tag="ACHALG_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313657.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase family
                     protein"
     gene            5113..5340
                     /locus_tag="ACHALG_001675"
     CDS             5113..5340
                     /locus_tag="ACHALG_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFN2587066.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            5356..6522
                     /gene="hemW"
                     /locus_tag="ACHALG_001676"
     CDS             5356..6522
                     /gene="hemW"
                     /locus_tag="ACHALG_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFN2587067.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            6756..7799
                     /gene="hrcA"
                     /locus_tag="ACHALG_001677"
     CDS             6756..7799
                     /gene="hrcA"
                     /locus_tag="ACHALG_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HFN2587068.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            7893..8456
                     /gene="grpE"
                     /locus_tag="ACHALG_001678"
     CDS             7893..8456
                     /gene="grpE"
                     /locus_tag="ACHALG_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFN2587069.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            8507..10336
                     /gene="dnaK"
                     /locus_tag="ACHALG_001679"
     CDS             8507..10336
                     /gene="dnaK"
                     /locus_tag="ACHALG_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFN2587070.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            10487..11653
                     /gene="dnaJ"
                     /locus_tag="ACHALG_001680"
     CDS             10487..11653
                     /gene="dnaJ"
                     /locus_tag="ACHALG_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFN2587071.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(11735..12364)
                     /locus_tag="ACHALG_001681"
     CDS             complement(11735..12364)
                     /locus_tag="ACHALG_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN2587072.1"
                     /translation="MGQLSDNQQLCQERINPLLELLERVFSYYGQALLTVHRQQIIIL
                     VNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSFLHAKQVCEWIAF
                     HQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLFKKTLACFSKNNG
                     SIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLLVQT"
     gene            12376..12549
                     /locus_tag="ACHALG_001682"
     CDS             12376..12549
                     /locus_tag="ACHALG_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587073.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            complement(12594..13331)
                     /locus_tag="ACHALG_001683"
     CDS             complement(12594..13331)
                     /locus_tag="ACHALG_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFN2587074.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(13333..14835)
                     /locus_tag="ACHALG_001684"
     CDS             complement(13333..14835)
                     /locus_tag="ACHALG_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFN2587075.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            15094..17088
                     /gene="uvrB"
                     /locus_tag="ACHALG_001685"
     CDS             15094..17088
                     /gene="uvrB"
                     /locus_tag="ACHALG_001685"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFN2587076.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            17098..19914
                     /gene="uvrA"
                     /locus_tag="ACHALG_001686"
     CDS             17098..19914
                     /gene="uvrA"
                     /locus_tag="ACHALG_001686"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFN2587077.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            20177..21442
                     /locus_tag="ACHALG_001687"
     CDS             20177..21442
                     /locus_tag="ACHALG_001687"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HFN2587078.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            21446..22177
                     /locus_tag="ACHALG_001688"
     CDS             21446..22177
                     /locus_tag="ACHALG_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HFN2587079.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            22312..23196
                     /gene="rapZ"
                     /locus_tag="ACHALG_001689"
     CDS             22312..23196
                     /gene="rapZ"
                     /locus_tag="ACHALG_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFN2587080.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            23193..24191
                     /gene="yvcK"
                     /locus_tag="ACHALG_001690"
     CDS             23193..24191
                     /gene="yvcK"
                     /locus_tag="ACHALG_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFN2587081.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            24225..25160
                     /gene="whiA"
                     /locus_tag="ACHALG_001691"
     CDS             24225..25160
                     /gene="whiA"
                     /locus_tag="ACHALG_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HFN2587082.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            25703..26353
                     /locus_tag="ACHALG_001692"
     CDS             25703..26353
                     /locus_tag="ACHALG_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587083.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            26540..>27101
                     /locus_tag="ACHALG_001693"
     CDS             26540..>27101
                     /locus_tag="ACHALG_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HFN2587084.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
BASE COUNT         8933 a         4570 c         5721 g         7877 t
ORIGIN      
        1 aaaaaatagc gctagaattt ccaacatctc tggaatttct agcgcttttt aattgtttaa
       61 gatacaaggg aaatatctgt ctggatcaat aatttttcat tgatccattg actaaagttt
      121 acgacataat tgaagttacc accgattagt atctccgcat gattgtcttt ataaagatcg
      181 tattgtaaaa gacggtcttt tttatttgcg taaacatcgt tatggtactg gactaattgt
      241 tctcggaatt gattgattgc tgtaaaatca cgatattgaa ggttgaaacg catcgtgatt
      301 ggtgcattta gcatgaatcg ctgcatttct tttgctgctt cctctgcttg atctccgtat
      361 aggaactcaa aaagattacg ttcagaaaat ttccattgcc cgccaacctt tgttcctttt
      421 agttttcctt catgaagata atttctgata gaacgtgtag taacacctaa ttgttcggct
      481 aattcattta ctgagtaaaa atctttcaat gttcaccctc ctatgtgaat ggttctactg
      541 acttagttga tatttgctta atcacgaaat gtagaaagaa cgattttaat taaggtcatt
      601 gtaaaaatca agtcagaaaa ttctaacttt tatttctcta aagaaattcg ttctctccta
      661 aagtgatatt atatttaaat atacctgaaa ataatgggca gtattatgaa agatcactga
      721 tattttctcg tattttttcc ttattcaaaa gttttgcagg aaaattgctg aaaaaataat
      781 tttactaagt agaaaattca aagccattca atcagtgatt tttttcagca caagaaagac
      841 tccttataaa aatagcagat actttgtcaa ttaagtgttt aaccagggat gataaaaagg
      901 ataggattga acaaaacctg tataaggtga tgaatagttt cctcgccagt aaaaggttta
      961 ttcgtataaa gctttatcgc caaaatttat catgaccttt tttatcttca atattaaatg
     1021 aaatcaattg ttcaatcaat tcccagttga ttacactttt ccaaggaact ttgatgacgt
     1081 tatccgtata tacgtagcct gcttcttgga tatttttttc aagggcacga atcgctgctt
     1141 tttccggtgc gatagaaaaa tgatttttag caaaactaaa tgcaataata aaggtaccat
     1201 ggtcagtaaa cataggttgg ttccatttta cagttgtgtc tagctgggga tacgtttctg
     1261 ttatcttagc aaaaaggtct ttgatctttt gttgatgttc tttttctttg atttgattga
     1321 tgatcggctg aaatttttcc attctaaaac gcctccatta agtggtttga tttattttac
     1381 cagaaaaaca atagttatgt ataaaaaacg aacaaaacac aagtaatgtt gtaaccgcta
     1441 tatttatatg ataagatgtc tcaaataata aaatagaaaa gagacataaa aatgattgac
     1501 ttggcaaaga aattcttaca cgaaaatata tattttgagt tgtatcatca tcgagccatt
     1561 tatacaaatg aagatgcttt ggtaataaag aaagaacaag gatttacagg tacagagacc
     1621 aaaagcttat tcttaaaaga taaaaaagat aataactacg tatatctgac ttttactacg
     1681 aaacaaacgg atttcaaagc attagggaag atcgtcggta aaaggctttc tattgtttca
     1741 tcagaacgca tggaagaaca aacgggacaa aagcagggcg ctgtttctcc gtttggttat
     1801 gaaaaacaga ttccgattat tattgatgaa gagctcttat caaatgaaaa acttgttttt
     1861 gcaccaggta gaccagatca aacgatggtc atccaaacgg ttgacttaaa caaaatcatc
     1921 cagctacttg agatgccgat atttttgatg ccggcaaacg aaaaaaacaa ttaaccaagt
     1981 aaaatagtgc atacttggag gtgaagcaac atgataagtt atccattatt agaaagaaat
     2041 gcaacgattg gggttactgc accatcctca ggcgtatcag aggaacacca ttcgttactc
     2101 gaatcagctt taaagagaat ggaagaaaaa tctttccaag tagttactgg aaagacgctt
     2161 tggacgcaag ataaggaaaa atcaggtcca gcaagaaaac gagcagatga attcaatcaa
     2221 atgatgcaag atgaaaatat ccaaatgatc tttccgactt ggggaggaga attattgatt
     2281 gagattctcg attttgtcga ttttgatgct ctacaaccaa aatggatagt aggttattca
     2341 gatattagta cgttgctact tgctgtcaca ttaaaaacag ggatagccac tgcacatgga
     2401 actaacctta ttgatttaag aggagagtac ttggatgaaa cgacagcaat gtgggaaagt
     2461 gtactgaaaa caagagaagg agaatcgatt ctacagcaat cttctgaaaa gtatcaaaaa
     2521 gtgtggcccc atgagcattc atcatcttct gttttccatc tcactgaacc aacaaattgg
     2581 caatcagtca gcggagatgc agtaaggatg aagggacgat tattaggtgg ttgtattgat
     2641 acgatcaggc atttaatcgg tactccttat ggagatgtcc aatcttttaa aagaaagtat
     2701 aatgatggaa agccgatctt gtggtttcta gaaaactgtg agctatcgtc agttgattta
     2761 agaagatcac ttgttcagat gaaactggca ggctggtttg atggcagctc ggggatcatg
     2821 ttcggtagaa gtgcggcaga ggatttgtta gattataaaa tgaaggatgt atacgaagat
     2881 ctgtcaaagg aattaaaact accgatcttg tatgatattg attgtggtca tgtaccgcca
     2941 caactgacat tgattaatgg tgcctatgca gaagtagcgg tagaagatag aaaagcgact
     3001 gttttgcaaa cgtttattta aaaaattttt tcctgccttg gagtctatca aagaccaata
     3061 aatctaaata gaaaagaaag tgaaagaagc tgacaaaatg ctgtcagctt ctttttgtat
     3121 aacatattag aaagtctaat tatctataac tgtcacatcg attaactgtt tcacgtcatt
     3181 gggatttcct gtaggaatag ctactacaga tgcattatca atgatttctt gagttgtttc
     3241 tctaatgaac gctggataag atgtttgatc tgcaggcatt tgccaatgtg aaggaactgg
     3301 ataactagtg attgtcccgt catggttact tgaatacact aaaactccct ggtaactgta
     3361 tttcccagtg agcataacca catctttcgg atagactgca ctccctgcat cataaggggc
     3421 gataggtgtt cctgcaggat aatgaataac atgtaattcg aaatcacccg atgtatctct
     3481 aaaagtatta cctctaatag ctagccaaac acgtgcgtac tcaatttgta gatcagaata
     3541 tcctgctaac ggatctattg actgagaaga ctgtgaggag gttgtgtcta ttgttgaaga
     3601 agaaatattt gtctcaactg tactactaga tgtagaagaa gtattgggct ctgagacttt
     3661 actgcttgtt gaagatgtat tcgtttcagt agaagctgat ttctgagtac ttgatttttc
     3721 agctgaactg gtttgtctga ttgaactttt gtctgtggtc gcagatggat cactattttc
     3781 tttactagga gacgtacatc ctacaaggaa taaagagacc aacaataaaa taaaacctaa
     3841 ctttttcata taaaatacct cattattttt ttaataccag tatagcataa tcctgaattt
     3901 ttgaatccgt aaaaccttgt ctatgaatat aaatagagtg tatcttcttt gataaatgtc
     3961 agaaaaagtg tgacaaataa tcatcagtaa aattacttgt cacactattg ataaggagta
     4021 tttaattgaa gagaatgatt aggagaaagg atctccgtac tttttatagt aatcatagaa
     4081 tccaagtttg tatagacgaa tcgttttttc tcttatttct tctgtttcaa aatggactgc
     4141 ttgaagaatc aagtctgtga attctatagg agctgttcca ttcgcagtaa tcaaattacg
     4201 atctttcaca gcttgttgtt ctataaaatc ttctttattc tgataatttt ccgattctaa
     4261 ccaaggaaat tgcgcatttc cggtgtgttt gtaaccttgc aataatccgc tctgagctaa
     4321 ataatcaact gccccacaaa tagcagctac tggtacgtct gtttttaaca atttatcgat
     4381 tatttgatat aaatgatgat tttttatgtc ccatgaattg ccaccgatca taataaaaag
     4441 gtcaactttt tcaggtattt gttcgattaa gtaatcgatt tttgttttta atcctccaat
     4501 ggaagtgacc tctgattgga tagaagcagt tttgttttgc catttctcat tttggtttag
     4561 tgtgctagta atataagcac cttcccaatc ggcatattcc tctaatagaa acgtaatcgc
     4621 agttttcatt ttcaagctct cctttactat ctataacaag gacaacagtt ttaacctaca
     4681 gacattcatt aaaattgggt agcgacagcg aaaacaacaa ttcttaagtt ttagacagag
     4741 tatacagata gtcaacttaa ttaaatacga actaaataaa aatgacaggt aatcattttt
     4801 gtttaccagc attataacat tttgcataca tggatgacaa aagaaccaat tcccacttgc
     4861 ttgagtacaa tattaaaaga aactggaagg caaaaagtaa ttatagaaac gaaaaataaa
     4921 tcatatcggg cactcagaca tgaaaacaac cctagagata tataactagg taaccagttt
     4981 aatgaaagaa aaaatcatcc aagaagtcga ttcttggatt ttttagtgga tttgttcgcc
     5041 aaatacgttt agtatgcgta tatgagaggt tgtaagcaaa gcgttgtggt ctgagcactt
     5101 aaggaggaat ctgtgaaaat agcttttcat atttttgcag attttgactt aatgccgaag
     5161 gtcagctttg cgattaccgt ttatcgagag gctgtaaaag caccgtgtag ctccgatgac
     5221 aataagcaag catttttaga gctaggtttc cttgattcta aatatgcggc ttatttcaat
     5281 gagttggtgc ttgaacgcga ttttaagaag gtcgaaaaca ggcacgactt ttcgacgtaa
     5341 ggagaaagaa tttcaatgat agaaacaata aatcccgcag aaaacaaggt ttctgcgtac
     5401 attcatatcc cattttgcga gcatatctgc tattactgtg attttaataa agttttttta
     5461 gaagggcagc cagtagatga gtatgtggaa atgctgctga atgaaatgaa aataatgcta
     5521 gaaagtcatc cgacagcgaa tttagaaaca ctttacgtag ggggcgggac accaacttct
     5581 ttatctgcta aacaacttga tcgactgtta tcaggtgcta gagagatcct gccttttcaa
     5641 aatggtaatg agtttactgt agaagcgaat ccaggtgatt tgacccgtga aaaacttcaa
     5701 gtaatgaaaa actatggcgt caatcgttta tccatgggag tgcagacatt tgataatcgc
     5761 ttattgaaaa aaattggacg taaacacacc gcagaagatg tgtatcaaac tatgcaattt
     5821 ttagaagcag aaaattttac caatgtaagc atcgatttga tttatgcatt accgggacaa
     5881 acactagaag gatatctgga tactttggaa aaagctttgg ctttggatct gcctcactat
     5941 tctttgtact cattgatcct cgaaaacaaa acaatgttca tgaactgggt acgacaagga
     6001 agactcgaac tgcctgatca ggaaacggaa accagaatgt ttgaagagac gattgaagca
     6061 atggaaaagc atggacgtca ccaatatgaa ataagtaact ttgggctctc cggacatgaa
     6121 tctcaacata acctcatgta ctggaacaat gaccactatt ttgggtttgg agctggtgct
     6181 agcggttatc ttggagatat tcgttaccgc aacaaaggac caatccaaca ctatttacgc
     6241 ccattacgag caaatgaacg cccggtattg gaagaagaaa aattgacacg aaaaaatcaa
     6301 atagaagaag aaatgttctt aggactaaga aaaaaaacag ggattactaa aaaacatttc
     6361 tatgatcgct atcaaaaaac aattgaatcg gtgtacggtg atgtgctaag ggaattagag
     6421 caagaagggt tacttgttaa tgaagaagac cgtgtttatt tgacaccaag agggaccttt
     6481 ttggggaaca atgtgtttga gcgctttttg tttgaaaaat agtttaaaga atatttgttt
     6541 tttatctgaa tttaaataaa aaaagttcaa aaatacaata aaggactatt gaaacgttga
     6601 tataactgga tttacatcaa ttatttagaa ttagcactct tgtgtttaga gtgctaaaaa
     6661 atggagtctt tctcttgaca ggagaagggc ttcttgctat atttataagt ggattagcac
     6721 ttagaatata tgagtgctaa tgagaggtga ggaagatgtt aacaaagcga caaaacgaca
     6781 ttttacgtct gatcatccaa cactatacga gtagtggcgt gcccgttggt tccaaaacat
     6841 tgatgtctga gggagtcaag gccagctcag caacgattcg caatgatatg aaagcattag
     6901 aagatgaagg attgctgttg aagactcact cttcttctgg gcggattcca tctgctttag
     6961 gctatcgtta ttacgtggat catttgttaa aacctgctcg cgtagcagaa gatgatttgc
     7021 tgctgattcg tcagtctcta ggcagagaat ttcatgaaat caatgagatt attaagcaat
     7081 ctgctgagat attatctgag ctgacaagct atacgacttt ttcattggga cctgaaataa
     7141 aagaccgccg actgactggc tttcgtatcg tacccttgaa taatcgacag attattgcta
     7201 tcgtcgtaac agatatgggg aatgtagaaa gccaagtgtt cactcttccc gaatatatgg
     7261 gaagtcagga actagaaaaa atggtacaga ttgtaaacga ccgtctagtc ggagatcctc
     7321 tgatgacggt gtaccataaa cttcgtacgg agattccgat gattcttcat aaatatttcc
     7381 aaacgacaga agggatgtct cagttatttg acaccgttct tggtcatgtt tttgaagata
     7441 aggtttatgt cagtggacaa atgaatctct tggattatga gccccgtcaa gatcttgatc
     7501 agttcaaatc gatgttttct tttatgaaag actcagacgg acttacacaa atgatcactc
     7561 cgatggatag caatattcat atcaagattg gtgctgaatt aggcaatgat ttattgcaga
     7621 atatgagtat gattcaagca agttacgaga tagatggtca tggaagaggt acaatagcct
     7681 tgttagggcc tactagtatg ccttattctc ggatgttcgg tctagtcgat gtttatcgaa
     7741 gagaactagc agctaaactt gctgattact accgttcgct tgacttgtca aactcgtaat
     7801 gtgaataacg aagctatttg cagctgatcg ttctctcttt cttagtaaga gcaatcaaac
     7861 agctgactga ataatgaaag gaagactgta tcgtgaaaga aaatcaagaa gaactagata
     7921 aagaaatgac agatgctcag ccagaaccgg aaatcgatgt cgaagctgca gaagattcgg
     7981 gtatttcaga agctgaagcg gaagaattcg aaacagcgaa attaaaagct gagttagaag
     8041 aaatggaaga taaatacttg cgggctcgtg ctgaaatcgc aaatatggca aatcgaggaa
     8101 aaaatgagcg tgagcaatta caaaaatacc gttcacaaga tttagccaaa aaattacttc
     8161 catcaattga caacctggaa cgtgcattag ccacagaagt gtcagatgat caaggggcag
     8221 gtttgaaaaa aggcgtggaa atggtcttgg aaagtttgcg taacgcactg gaagaagaag
     8281 gaattgaaaa aatccctgct aaaggagaag cctttgatcc taatcttcat caagcagtac
     8341 aaactgttcc agcgactgaa gatacaccag ccgatacgat tgtagaagta ctacaagaag
     8401 gctacaaatt acatgatcgc gtgttaagac caaccatggt cattgttgca caataattgt
     8461 gcggatataa aacttacact ttcaattaaa gaaggagacg ttaattatga gtaaaattat
     8521 cggtattgac ttaggtacga ctaactctgc agtagcagta ttggaaggaa acgaagcaaa
     8581 aatcatcgct aacccagaag gaaaccgtac aacaccttct gttgtatcat tcaaaaatgg
     8641 agaaatccaa gtaggggaag ttgcaaaacg tcaagctgtg acaaacccaa atacgatttc
     8701 atcaatcaaa cgccatatcg gtgaagcagg ttacaaagta gatgtagaag gtaaatcata
     8761 tacaccacaa gaaatctcag caatgatcct tcaatatatc aaaggattcg cagaagacta
     8821 tttaggtgaa aaagtcgaaa aagcagttat cactgtgcca gcttacttta atgatgcaca
     8881 acgtcaagca acaaaagatg ctggtaagat cgctggtcta gaagtagaac gtatcgtcaa
     8941 tgaaccaact gcagctgctc ttgcctatgg tttagataaa acagatcgcg acgaaaaaat
     9001 cttagtattc gaccttggtg gcggtacttt tgacgtatct atccttgaac taggagacgg
     9061 tgtctttgat gtattgtcaa cagctggcga caaccatcta ggtggggacg actttgataa
     9121 caaaatcatc gactacatgg tagcagaatt caaaaaagaa aacagcattg acttgtctca
     9181 agacaaaatg gctttacaac gcttgaaaga cgctgctgaa aaagcgaaaa aagatttatc
     9241 tggcgtatca agcacacaaa tcagcttgcc atttatcaca gcaggagaat ctggaccatt
     9301 gcatttggaa atgacattga cacgtgcaaa atttgatgaa ttaacagctg acttagtaga
     9361 acgtacaaaa gtaccagtac gtcaagcgct aaaagatgca ggtttatcac aatcagaaat
     9421 cgacgaagtt attttagtcg gtggatctac tcgtatccct gcagttgtag aagcagtacg
     9481 taaagaaaca ggaaaagaac caaacaaatc agttaaccct gatgaagtag ttgctatggg
     9541 tgctgctatc caaggtggcg tgatcactgg tgatgtgaaa gatgttgttt tacttgacgt
     9601 tacaccattg tcattaggta tcgaaacaat gggtggagtg ttcactaaat tgattgaccg
     9661 caatacaacg attccaacaa gtaaatcaca agtattctca actgcggcag ataatcaacc
     9721 agcagtagac atccatgtat tacaaggtga acgcccaatg gctgctgaca acaaaactct
     9781 aggaagattc caattgacag atattccagc agctcctcgt ggtattccac aaatcgaagt
     9841 aacattcgat attgataaaa acgggattgt aaatgtatct gcgaaagatc taggtacaca
     9901 aaaagaacaa aaaatcacga tcaaatcttc ttcaggtcta acagacgaag aaatcgaacg
     9961 tatggtaaaa gatgcagaag caaatgcaga agcagataaa gctcgtaaag aggaagttga
    10021 cctacgtaat gatgtagatg cattgctatt ctctgtggac aaaacattga aagaattaga
    10081 aggcaaagtg gacgaagaag aagtgaaaaa agcagaaaca gctcgcgatg aattgaaagc
    10141 cgctgttgaa gcaaacaaca tcgaagaaat gaaaactaaa cgcgatgcat tgaacgaaat
    10201 cgttcaaaac ttgactgtta aactatacga acaagcggca caacaacaag ctcaagaaaa
    10261 cccagaagca gctcaaggcg gagcagatga tgtcgttgat gctgattttg aagaagtcga
    10321 tggcgacgat aaataatcag ttcactgaat ttcgagagaa ggccaaagtt gctactttgg
    10381 ctttttcttt tgaaaaatag tttgaagtat cggtttttgt ttcaaacaaa atatgatatg
    10441 ataacaagcg acgtgaaaat gatgagaacc aataggagga aagcctatgg caacaaaacg
    10501 tgattattat gaagtcttag ggctctcaaa aggagcatca gaagatgaaa taaagaaagc
    10561 ttatcgtaag ctttcgaaaa aataccatcc tgacatcaat aaagaacctg atgcggaaga
    10621 aaaattcaaa gaagtatcag aagcatatga gattttaagt gacccgcaaa aacgcgctgc
    10681 atatgaccaa tacgggcatg caggcacaga tccaaattac ggtgctggtg gagctggtgg
    10741 cggatttggt ggattcggcg gcttctctgg tggcggcttc ggtggttttg aagatatctt
    10801 cgactctttc tttggaggag gcggtcggtc agtcgatcca aatgcgcctc gtcaaggtgc
    10861 tgatttgcaa tatacggtcg acttgtcttt tgaagaagcg atttttggtg tagaaaaaga
    10921 aatcaagtat aatcgggaag aaatctgtca tacttgtggt gggaacggtg caaaaccagg
    10981 cacacaacca actacttgtc ataaatgtca tggtgcaggt acgattaatg tagaacgcca
    11041 aacaccactt ggacgagtga tgagccgtca aacttgtgat gtttgtcatg ggacagggaa
    11101 agaaatcaaa gaaccttgtc caacctgtca tggctcagga catgagaaga aagcacattc
    11161 agttaaagtg aatgtaccag ctggtgtaga agaaggacaa caaatgcgtt tagctggtca
    11221 aggagaagct ggagaaaacg gcggaccatt tggtgatttg tatgtggtct tccgtgtaga
    11281 agaaagtgat atctttgatc gtgaaggttc tgaaatttac tatgaattgc ctttaagttt
    11341 tgtacaagca gctttggggg atgaagtcca agtaccaact gtccatggag atgtgaaatt
    11401 gaagatccct gctggaacac agacaggtac gaacttccgt ttacgtggaa aaggcgctcc
    11461 gaagttacgt ggtggtgcaa caggcgacca acatgtcaaa gtaaaactga tcacaccgaa
    11521 aaatctaaat gaacaacaaa gagaagcatt acgtgcattc gctgaagctg gcggcatcaa
    11581 agttgaagaa cagcaagaag acggtttctt cgataagttt aaagatgctt ttggcgggaa
    11641 aaagcggaag tagtttttct agtaaaaacg ctaagtcatt aataattaaa aaccagacga
    11701 acaatcatat gttcgcctgg tttttttgta gttgtcatgt ttgtaccaac aaaaaagcca
    11761 gctttagaat atgataatcg tcatagtttc taggggtata accagtgaga gaatggaact
    11821 tgttcaatcg atattgcaag gtgtttttat gaataaataa ttcttctgag caatttttga
    11881 tgcttccgtt atttttactg aagcatgcca aagtcttctt gaacaaatgt acttcttctt
    11941 ctgtcaggct tttcaaaata cgttttacat ataatgttcg ttgatctgac gggattgctg
    12001 gtaaaacgat cccgaggtct aaatcttcaa aaaagcggat ctcgttcaca gattgatgga
    12061 aagctatcca ttcgcaaact tgtttggcat gaaggaaaga ttgcggtgtc tcttgctctg
    12121 tataacaaag cgacccaatt ccaaagttta gctgtaactg atacatttta ttaaactttg
    12181 tctggatttt atcaagcaat gatagcaatg aggctctgga aatccgattg actaggataa
    12241 tgatttgttg tctatgtaca gtgagcaaag cctgtccgta ataagagaag accctttcga
    12301 gcaactccaa taaaggattg atccgttctt gacacaattg ctgattgtca gaaagttgtc
    12361 ccacaactac ataatatgga gaagaaaaat caagctcaat taagtgaggg tggtatcgac
    12421 tatgtacttg atatcatcaa tgaaccgatg gtactttctt atgcgatgga acaggtagag
    12481 cgattgcttg tttgtgcagg aaaacaagcg atgcggctga tacgattagg tgagaaaata
    12541 agaagataaa aatacacggt cccttcatgg cgaccgtgca tttttggttt ttattaaata
    12601 tttaatactt tattcaagaa atcttttgtc cgttcatttt gcggattttc aaagatttgt
    12661 tgaggcgttc catcttctaa aaagtttccg tcgtcaatga acatgacgcg attggccact
    12721 tctttagcga accccatttc gtgcgtaacg ataaccatcg tcataccttg ttctgctaac
    12781 tttttcatga cgttcaagac atcaccgacc atttcaggat cgagtgcaga agtaggctca
    12841 tcaaaaagca tgatgtcagg gttcatcgct agagcacgag caatagccac acgctgtttt
    12901 tgtccaccag aaagcatttc aggatacata tcttttttat cttctagacc aactgtctgc
    12961 agtaatttga ttgctttttt ctctgcttct tctctagatt ctttcttcag gtccatcggt
    13021 gctaaaatga tgttttccaa aatagaaaga tgagggaata ggttaaagtg ttggaatacc
    13081 attccgatat gttgtctcac caagttgata tctgtgcttt tatccatcaa gtgggcgccg
    13141 tcaataataa tttcaccgtc agaaggttct tctaaacgat tcaagcaacg caagaatgta
    13201 ctttttcctg aacctgaagg gccaatgata cagacgacat ctccttcgtt gatggataca
    13261 ttgatgtcgt ttaatactgt gttatcgccg tattttttga ctaaatgctc aactaagatt
    13321 ttttctgcca tcttacttca ccttcttttc taaaactttt gctaattttg tcaatgctgt
    13381 gatcaaaatc aagtacatga tcgcaatgat caagtaaaca tacgtacttt gtgttgtacg
    13441 tgcaacaatg atcttaccag tttgcagcaa ttcgactaca ccgattgcgg aaatgatcgt
    13501 tgtgtctttt aatgagataa cgaactgatt gacgaatgat gggatcatga tgcgtatcgc
    13561 ttgcggcaaa atgatttttt gcattgtccg attataagaa agacctaaac ttctggaagc
    13621 ttccatttgt ccgacaggaa cggcgttgat cccgcctcga acaatctctg cgatataagc
    13681 acttgcgtta agagtcaagg tgataacacc agcaaggaag tcaggaatcg taaatccaac
    13741 gataccaggc aacccaaaga aaataaagaa cgcaagaacc atcattggaa ttcctcggat
    13801 gatatcgaca taaatactag caattgtgcg aagagcgcgt attggtgcta cactgaataa
    13861 tccaaaaatc acaccgatta ctatagccaa agcaaaggag atcaaagtta ggagaattgt
    13921 tctccaaagt cctttcaaca atgcttgata gttgttttgg ataagtccaa gaaatgttga
    13981 ttcatcagca gtggaagact cactggaagt ttctgaacca tccgctacat attctttaat
    14041 aatcttatcg tattcgcctg ttctttttaa ttctttcaat ccttcattga acatattaag
    14101 aagctcagga ttttgtcctt ttttaacggc aaatgcatat tttccgccag attctttttt
    14161 gatcggtgtt tccaattctt gtccttgttt gatcgcataa ccgattaccg gataatcatc
    14221 catcattgca tcgattgcgc caacttttaa cccgtcgtac aattgatcag cagcatcaaa
    14281 atatttgata gaatagccat attcagattc atggtcgctt aaaaattcag cactttcggt
    14341 accaactttt gctccgactg tttttccttt caaatcttca taagacttga tcgaagtatc
    14401 tccttttttg acagctaatt ggatcccgct atcaaagtaa gaatcagaaa aatcatagct
    14461 tttctttcgt tcatctgtta cagtcattcc ggcaatcatt ccgtctgctt gtcctgattc
    14521 caaagcttgc aaagcagcag agaaaccacg gtagtccatt cggatgttaa atccttgcag
    14581 tttggcgatg ttttccatca attttacatc gatgccttcg tactcgccct ttgcgttttg
    14641 aaattcaaat ggagcaaaag cagagtcact ggcaattacg tatacgtctt ttttgggctc
    14701 tattttttgc attgtctcgc ttgtgcttga ctctgccgtt gtagtgttgt cagcttgtgc
    14761 aacatgacca aacgcaagtg tcacaagtcc tgtcattaag aaaacaaatg agtgaaataa
    14821 atgctttctt ttcataatca acctcctaaa tttccaatat tttattatta taggcgaaaa
    14881 gtgctgaatc agacaagact gtaagctcga aaaaatgaaa aaaatgcgag tttctaacat
    14941 tttatgttat aaaacataac atgtataata agataaaaaa gtggcgaaag tatgttcggt
    15001 gtggtataat tttttagtaa ggaatgcctt tgctttcctt ttctttttat tggtcgtaaa
    15061 atgaaaagga atgaatgaaa aggaggacga agaatgattg agagagatac ctcccgacac
    15121 tttgagcttg tctcgaaata ccaaccggct ggtgatcagc ctgaagcgat taaccaatta
    15181 gtggatggcg tagtcggcgg gaaaaaagca caaatcttat taggtgcgac tggaacaggg
    15241 aaaacatata cgatttccaa tcttattgag aaagtgaata aaccaacact gattattgcc
    15301 cataacaaaa cattggctgg tcagctgtac ggggaattca aggaattttt ccctaacaat
    15361 gctgttgaat attttgtttc ttattatgat tattatcagc ctgaagctta tgtgccttca
    15421 agcgatacgt atatcgaaaa agattcaagt gtaaatgatg agatcgacaa attacgacat
    15481 tcggctacta gttcattatt agaaagaaat gatgtgatcg tcattgcatc cgtttcatgt
    15541 atttttggat taggctcgcc ttttgagtac caaaaacaag tagtttcgat tcgacaggga
    15601 gcagaactag atcgcaatca gttgattcgt gacttagtca gtatccaatt cgaaagaaat
    15661 gatatagatt ttcagcgcgg tcgttttcga gtgcgcggtg atgtcgtgga gatttttccg
    15721 gcttcaagag atgaacgtgc cttacgtgtg gagttttttg gagatgaggt cgaacgtatc
    15781 cgagaagtca atgcactgac aggtgaagtt ttaggagaaa cggaacacgt agcaatcttc
    15841 ccggcgactc actttgtgac gaatgatgaa catatggagc atgccgtagc gaatatcaaa
    15901 gcggaattag aacagcgttt aactgtcttg cgaaatgaaa acaaacttct ggaagctcaa
    15961 cgtttagaac agcgaaccaa ttacgatatt gaaatgatgt tagaaatggg ctacacgtcg
    16021 ggaattgaaa attattctcg gcatatggac ggacgtaaag aaggtgaacc gccttatacg
    16081 ctattggatt tctttcctga agattttttg atcgtggcgg acgaatcgca cgtaaccatg
    16141 cctcaaatca gaggaatgta taatggtgac cgagcgagaa aacagatgct agtagattac
    16201 ggtttccgtt tgccatctgc tttagacaat cggccacttc ggttagaaga gtttgaaaaa
    16261 catgtcaatc agatcattta tgtctctgcg acgccaggac catatgaaca tgaacaaaca
    16321 gataccgtta tccaacaaat catccgtccg acagggctgc ttgacccagt gatcgaagtt
    16381 cgtccaatca tgggacaaat cgatgatcta gtaggcgaaa taaatgaacg agtcgaaaaa
    16441 gaccagcggg tgtttgtaac gacattgacg aaaaaaatgg cagaagacct cacagattat
    16501 ttcaaagaat tagggatcaa agtcaaatat ttgcatagtg atatcaaaac gctggaacga
    16561 acagaaatca ttcgagatct tcgattaggt gaatttgatg tgttagttgg aatcaatttg
    16621 cttcgtgaag gattagacgt acctgaagtt tcccttgttg ctatcttgga tgcggataaa
    16681 gaaggctttt tacgtagtga acgttcctta gtccaaacga ttggacgagc tgctcggaat
    16741 gaagaaggta aagtaatcat gtacgcagac aaagtaacag attctatgcg tctagcgatg
    16801 gatgaaacgt caagacgtcg tacgattcag cagaagtaca atgaagaaca tgggatcgtt
    16861 ccaaaaacga tcattaaaga aatccgagat ttgatttcta ttacgaaaga atcggaagat
    16921 gatacaaaag aagcggtaca agtctcgtac gaagaaatga cgaaggaaga aaaagacacg
    16981 ttattgatga aattagaaaa agaaatgaaa gatgcggcaa aagctttgga ttttgaaaca
    17041 gccgcgaatg tgcgagatat gattttagaa ttaaaagctt caaagtagga ggacaacatg
    17101 gctaacgata aaattacgat tcatggtgca cgtgcccata acttgaaaaa tatagatgta
    17161 acgattccac gagataaatt cgtggtcgtt acaggtttgt ccggttctgg aaaaagttcc
    17221 ctagcatttg atacgctgta cgctgaaggg cagcggagat acgtagagag cctttctgct
    17281 tatgctcgtc agtttttagg acaaatggat aagccggacg tagatagcat cgacggattg
    17341 agtccggcga tttctattga tcagaaaaca acaagtaaaa atcctcggtc gacagtaggt
    17401 actgtgacgg aaatcaatga ctatttacgc ttgttgtttg cacgtgtagg acatccgatt
    17461 tgtcccaacg atcatatcga gattaccagt caatcagtag atcaaatggc agatcaagta
    17521 atggctttgc ctgagcgcac aaagatccaa gtgttggcac cgatcgtggt aaagaaaaaa
    17581 ggacaacata aaaaaatatt cgaacgaatc caaaaagagg gatacgtccg cgttcgagtc
    17641 gatggtgaga tctatgattt aagtgaagtg cctgagctgg agaaaaacaa aaaacatgat
    17701 atcgcgatcg tcatcgaccg gatcattgtg aaagaaggga ttcgttctcg cctatttgat
    17761 tctttagagg cggccctgcg attagcagac ggctatgctc tggttgacgt gatcggtgaa
    17821 gaagaaatgt tgtttagcga acactatgcc tgtccttatt gcggatttac tgttggcgaa
    17881 ctagaaccaa gactgttctc attcaatgcg ccttttggag cttgtccaga ctgtgatggt
    17941 ttaggtgtca aattagaagt cgatcaagat ttagtgattc cagatagaac gaagacactt
    18001 agtcaaggtg ctatcgttcc atggaatcca atcagctctc agtattatcc tcaaatgtta
    18061 gcgcaggctt gtgaaagttt cggtatcgat ttagatacac cattcgaaga attaccggaa
    18121 gagcatcagg agatcatctt gaatgggaca gatgatcttt tccatttcca ttatgaaaat
    18181 gattttggtg gcgttcgcga tgtagaaacg actttcgaag gggtattgaa gaatatcgag
    18241 cgtcgttatc atgatacgaa cagtgatttc acgcgtgagc aaatgcgtct ttatatgaca
    18301 gaattaactt gtcagacatg taaaggttat cgattgaatc cccaagctct atctgttcaa
    18361 atcaatggaa cgaatatcgg gcaaacaaat gagttgtcta ttcaaaaagc agtagatttc
    18421 ttctcaaact taacgttatc tgaacaagag aaagtaattg ccaaaccgat tttaaaagaa
    18481 gtaaatgaca gactttcatt cttggaaaat gtgggactag actatctaac tttgagtcgc
    18541 gcttccggaa cattatctgg gggagaagcg cagcgaatca gattggctac acaaattgga
    18601 tcgaatcttt ctggagtttt gtatatttta gatgaaccat cgatcggatt gcatcaacgc
    18661 gacaacgatc gactgattgg ctcattaaag aaaatgcgtg atcttggcaa caccctcgtt
    18721 gtagtcgaac atgatgaaga tacgatgtgg gcagcagatt atttgattga cgtcggacct
    18781 ggggccggag aacaaggcgg acaaatcgtg gctgcaggca ctcctgaaga agtagctaat
    18841 gatgaaaatt ctttgacagg gaaatatctt tcagggaaaa aatcaatccc tgtaccaaaa
    18901 gaacgccgta aaggtaatgg gaaagcaatc aagatcactg gtgcttcaga aaacaatctg
    18961 aaaaatatta gtgttgaatt tccattagga gaatttgtag cagtcacagg tgtgtctggt
    19021 tctggaaaga gtacattggt caatagtatc ttaaagaaat cgttagcgca aaaattaaat
    19081 aagaattctg ctaagccagg taaattcaag acaatttccg gctacgaaag tatcgaaaag
    19141 atcatcgata tcgatcaaag cccaatcggc cggacgccga gaagtaatcc agcgacttat
    19201 acaagtgtat ttgatgatat ccgtgggtta tttgctcaaa cgaacgaggc aaaaatgcgg
    19261 ggttataaga aagggcgttt tagtttcaac gtaaaaggcg gtcgttgtga agcttgtcgc
    19321 ggggatggaa ttattaagat cgaaatgcac tttttgcctg atgtctatgt tccttgtgaa
    19381 gtatgtcatg gtaaacgata taattctgaa acattagaag tgcattacaa aggaaaaagc
    19441 attgctgata ttttggaaat gacagtagag gatgctgtag aattcttcaa gcacattcca
    19501 aagattcatc gcaaactgca aacgattgtt gatgttggct taggttatgt gacgatgggg
    19561 caaccagcaa cgacattgtc cggtggtgag gcacaacgga tgaaacttgc cagtgaattg
    19621 cacaaaatct ctaatggaaa gaatttctat atactagatg aaccaacgac aggacttcat
    19681 agcgatgaca tcgcccgctt gttgcatgta ttacaaagat tagtagatgc tggtaacaca
    19741 gttttagtga ttgaacacaa tctagatgta atcaaaacag cagattatat cattgattta
    19801 ggaccagaag gtggagaagg tggaggaacg atccttgcga ctggaacacc agaagaaatc
    19861 attaacgtaa aagaaagtta tacaggtcac tatttgaaaa aaataatggt ataaagagga
    19921 tagatagctg agatagttaa aaaaacataa aaaaatgaat ttttgcttgt tttattaata
    19981 tcagtgttct agaatcatta catggtagtt ctttctacac tgattggtat agataaaaac
    20041 ttcctataag ttcaatccgt tcaaaaaaga attgagcaat cggaggactt tcctttgatt
    20101 gcttttttct ttatggtatg atattccttg tgtagttgga aaagaaaaac aagagattat
    20161 gagtaagtga gagataatga tgaacagtat tgaaaatgaa gaatttatcc ctatattatt
    20221 aggaagcgac atgaacgttt atggtatggc acgttctttt aatgaagcat acggcaaaat
    20281 ctgccaagca tatgcttcgg atcagttagc ccctacccgc tacagtaaaa ttgtcaatgt
    20341 agaagttatt ccaggatttg ataaagaccc tgtatttatc gagacgatgc ttcgtttggc
    20401 aaaagaaaga tattctgata agagtaaaaa atatcttttg attgcttgtg gtgatggata
    20461 tgcagaattg atctcccaac ataaacaaga gctgtcagaa tattttatct gtccatatat
    20521 tgattattct ttatttgaaa ggctgatcaa taaagtaagc ttttatgaag tttgtgaaga
    20581 atatgatctt ccgtatccta aaacattgat cgtcagagaa gaaatgttag taaatggaca
    20641 tttggaacag gaattgcctt ttgaattccc agttgctttg aaacctgcaa acagtgtgga
    20701 gtatctttct gtccaatttg aaggaagaaa aaaagctttt attttagaga ctagagaaga
    20761 gttcgattta attttaggcc ggatatacga agccggttat aaaagtgaaa tgattgtaca
    20821 agattttatc ccgggtgatg atagtaatat gcgtgtgcta aatgcgtatg tagatgaaga
    20881 tcatcaagtt cggatgatgt gccttggtca tccactatta gaagatccta caccagcatc
    20941 gattggtaat tacgttgtga tcatgccaga ttataacgaa aaaatctatc aaaccattaa
    21001 ggcatttttg gaaaaaatcg aatatacagg ctttgccaat ttcgatatga agtatgatcc
    21061 tagagatgga gaatataaat tatttgaaat caatttgaga caaggtcgta gcagtttctt
    21121 tgtgacattg aacggcttga atctggctcg ttttgtcaca gaagatcgag tgttcaacaa
    21181 accctttgtt gaaacgacct atggaacgaa tcaatcagat aaagcaagat tatggatggg
    21241 tgtgcccaaa aaaatctttt tagaatatgc tcgggaaaat gaagacaaaa aactagctga
    21301 gcaaatgatc aaagaaaatc gttacggtac tactgtattt tatgaaaaag accgatcaat
    21361 caagcgctgg ttgttgatga aatatatgtt ccataactat attccaagat tcaaaaagta
    21421 ttttcatgtg aaagaaggct agttcatgga gaattttttc agtattttag gcggaatggg
    21481 cacgatggcg acagaaagtt ttgttcgctt gatcaaccac cgaacgaaag ctacaaaaga
    21541 tcaagaatat ttgaattatg tcttattcaa tcatgcaaca gttcctgatc gtacagctta
    21601 tattttagat cgatcagaag aaaatccaat gccgtttctg ctggatgata ttgagaaaca
    21661 aaatttattg cgaccaaact tcattgtgtt gacttgtaac accgcacact acttttttga
    21721 agagttacaa gcggcaacgg atattcctat cttgcatatg ccaagagaag cagcaaatga
    21781 acttgtccgt cagcatacga cagggagagt agcgattcta ggaacagaag gaagtatgaa
    21841 agctggaatc tatgaacgcg aagtcaaaaa tcttggtttc gaaacagtga ttcctgatac
    21901 tgcgcttcaa gaaaaaatca attacctgat ctatcatgag atcaaggaat cggattattt
    21961 gaatcaagag ctctattacg aaatcttaga ggaagcggta gagcgcctaa actgtgaaaa
    22021 agtcatccta ggttgcacag aattatcttt gatgcatgaa tttgctgaag ataatcatta
    22081 cccagtgatc gatgctcaat ctattttagc ggatcgaacg atcgaacgtg cattagctga
    22141 aagaagcgag gcattggata cagcatcgga aaagtaaaaa aattggattt ggggcaaata
    22201 tcctaaaaga tatttggtcc aaatcttttt gtaaaagaaa aagaaaactg gtaatgatac
    22261 gcccgggatg atataatttt attatgcaca atgtgagagg ggttcgtaaa tatgacagat
    22321 aatttgcaat tagtcatcat aacaggtatg agtggtgcag ggaaaacagt agctatccaa
    22381 agttttgaag atatgggcta tttttgtatc gataatatgc cgcctcgatt gattccaaaa
    22441 ttctgggaat tgatcaaaga atcaggtaaa gtcacaaaaa tggctttagg agtggatttg
    22501 cgttctcgtt ccttctttga agaaatccaa aacatgctga tcgaaatcga aaatacgaac
    22561 ttcatcgaca cttctatctt atttttagat gcttcagacg aagaactagt ggctcgctac
    22621 aaagaaacaa gacgagcaca tccaatggcg atggacggtt tgatcactga agggatacgt
    22681 aaagaacggg cgatactaga cgacttgaaa gcccaagctt ccgtgatcat tgatacgaca
    22741 acattgacac cacgacaatt acgtgaaaaa atcaatgagt cattcaaatc aaagggagac
    22801 caaggatttc ggatcgaatt tgtatcattt ggttttaaat acgggttgcc gatcgattcg
    22861 gatattgtga tggatgtgcg ttttttgccc aaccctcatt atatcccgga actaagacca
    22921 ttgaccggaa aagatcagcc agtctatgat tatgtgatgt cctttgatga aacgcaatca
    22981 ttctaccata aatttttgga tcttctggaa actgtattgc ctggatacat taaagaagga
    23041 aaaagcagct tgacagttgc aatcggctgt acaggcggtc aacaccgttc tgtggcattg
    23101 actgaacgtt tggcaaatgc tttagggaaa aactataaag tcaatgtgac acatcgggac
    23161 aaggataaaa ggaaagagac ggtgaaccgg tcgtgaaaat gaaaacctac cggataagaa
    23221 aaccaaagat cgtagtggtc ggcggtggca ctggtctgcc agttatcctg aaaagtttac
    23281 gaaatcaagg agcagatatc acagcagtag tgactgttgc agatgatggt ggaagcagcg
    23341 gtgcaatcag agaatcgatc gctatggctc ctcctggtga cttgagaaat gtcttagtgg
    23401 cgttatcgga tatgccacaa ttctatgaag atattttcca ataccgtttc aaaaaggaag
    23461 atcagttttt agccaatcat acgatcggta atctgatcat tgccgcagtt tccgagatgc
    23521 ggggaagcac ttatgaggcg attcaattat tagcaaaaat gatgcatgta gatggacatg
    23581 tttatccttc ttcagaaact cctttgacct tgcatgctgt ttttaaagac ggaacgactg
    23641 cagtaggaga atctaaaatc gcgatcaacc gtaaaaccat tgaccgtgtg tatgtctgca
    23701 acacaaatga taaaagtgaa gccaaagcgg ctcgaaaagt cgtttcagca attatggacg
    23761 ccgacatggt cgttttggga cccggaagtt tattcaccag tatccttcca aatttagtga
    23821 taccagaaat cggcgaagca atgctcaaga caactgctga aaccgtctat atctgtaata
    23881 tcatgaccca aaaaggggaa acggaacatt tcacagatgc cgatcatata cgtgttttac
    23941 acaagcattt gaatcagcct tttatcgata cagtattggt caatacggaa aaagtgcctg
    24001 aagattatat ggacccagag atctatgatg agtatctagt acaagtcgca cacgacttca
    24061 acggattgag aaatgaagga tgtcgagtga tttccaccga ctttttagaa ttgaaagatg
    24121 gtggtgtctt ccacgatgga gacaaagtag tagaagaatt gttccgttta gttttcggtt
    24181 caaaatatta agcaatttat tgttggagaa aggaggagaa taggatgtca tttgctgcag
    24241 atgtgaagaa agaactaact ggtttggaag ttcatcgaga acatgcgaaa gcggaattag
    24301 ctgctttgct gcgaatgaac ggctctttag gtattgtgaa ccagcagttt attttgaacg
    24361 tccaaacaga aaatgctgca attgccagac gtatctattc tcttttgaaa gatcattatc
    24421 aggtacgaag tgagctactc gttcgtaaaa aaatgaaatt aaaaaagaac aatgtctata
    24481 tcgtccggtt gaagcaaggc acaaaagatg tgttgatgga tcttgatatc atggatggta
    24541 tgatgttcaa tggacacgtt tcggacgaga ttatgggaaa ttcgcaaaaa atgcgttctt
    24601 acttacgtgg tgcttttatg gcctcaggtt cagtcaataa tccggaaacg agtcgttatc
    24661 atttggaaat attttctatt tatgaacagc ataaccaaga catttgcgat atgttaaatt
    24721 attacggtct acatgctcga acgcttgaac ggcgcaatgg ctatatttcg tatttaaaag
    24781 gagcagaaaa aattgctgat ttcttaacat tgattggtgc aaccaactca atgctccgat
    24841 ttgaagatgt tcgaatcgta cgggatatga gaaattcggt caatcgtcta gtcaattgtg
    24901 agacagcaaa tctgaacaag acgattgatg cggcttctaa acaaatcgaa aacatcgaat
    24961 tgatcgaagc aaaggtcggt ctacatgccc ttcctgaaaa acttcaggaa atcgcagaac
    25021 ttcggcttca acatccagag gtcagcttga aagaattagg ggaaatgatt ccctcaggtg
    25081 caatatcaaa atcgggaatt aatcaccgaa ttcgcaaaat aaatgaattt gcggaaaaac
    25141 tgcgtgaaga agcagtttaa tccaaagaaa aaactgatac atgaagcagg agatgcgaat
    25201 gtatcagttt tttttaaaag ctatacttgc ttttcttccg tcaaactata attccaactt
    25261 tactggagta acataatgtt gaaaggcttc tattgttttt taatacaatc gtataaaaag
    25321 cggggagaca actgaatgaa gattttagtt ttatacataa acttttgctt aatatagaag
    25381 tcctgatata gataggatca agtatttatc tataactgga gattaattaa aaactggaag
    25441 gaactgctcg atcaaccttg agaactgttt ttcttacttc tggtcaagat tctcgtggtt
    25501 ggtggataag gatggttgat aagttcattg gattagctag tcgaaaacaa ccaggtgtaa
    25561 ttaatgtaga agttttaaca aggggatgga gtgatttatt cagtgataat cataagttca
    25621 taaatagtaa ttgggatgta tggttggtat acgttatacc tgaacttgaa ctatcaagat
    25681 ttggtttaga atttgagaat cgatgtttat aaagggaggg caaaaaatgt gcttttttac
    25741 aacttttact atcattatga ttgcagtact ttttattttt ctagatatag caaagcgaaa
    25801 tacagcattt ttattatatc gggttttact tcgagcaggt ctcataacat ttatatcgat
    25861 cgttggattt tttctattca cagttattgt atttatttgg agaactacag caccaccatt
    25921 gccagagatt acatatggag aatttccgtt tagtcttgaa tatgaaatag atgaggaaag
    25981 aattttgatt gaagatacgg tgattgcaga atttatagga agttttagag ggaatgtgac
    26041 aactcaacct agacgaagtt ggagtataaa acttttaaat aatagtggag agttgaaagc
    26101 atttcattat gaattcttaa taatagaaag tgaagatgta acaatcactt taagtagtgg
    26161 ctctgctgga tattttatga atgatggtgg aagcaagcca cagctcacag tccgtgatcg
    26221 tattgcaggt tttatgcatt tttctcacta tgacttagaa gaagctcgta aaatacttgc
    26281 agaatatggg atcacattaa tcaattggga atatgcgcca ccgataagaa attttttcaa
    26341 tgattattat tagtcttata aaaaacagaa tgtaaaattt atgtgcgcga gtatttagtt
    26401 ttccattttt tgtttgatag atatatagca ttgttgaagc atgggatagt ggtgccaatt
    26461 ttatcaatga ggttggtcag gcagttaaca taattggtga tgctataaat ccatttaatt
    26521 ggaattggag gaattaggtg tggaaacagt gagattatgg atattcatga aaaattttcg
    26581 ttcggctttg agatatgtta ttctggaatc aaaatcaggt tattatctga ttgacacaga
    26641 tcactatact ttttggggag gctacctttt tccgattttt aattggataa tcccccataa
    26701 ggttatgaaa attaacttgc ctgcagaaga aataaataga ttaatactca acaaagattt
    26761 acaaaggaaa agagagaaag aacattctaa tgttgcttgg ataggtccag catttgcagt
    26821 agtgacttct tcaattgtcg gtcctatgct agagagtatt tatagtgatt tttctattgg
    26881 aatacgttat ttatttgtta ctttaagtat agctgttctt ttgtgcataa agggttatgt
    26941 tagcaagaag aataaagata ttttaacact aatcggtgta aacaatttat ctagtactaa
    27001 aattatactg tttcctgaat ctataaaaga aggatcaagg ttgttgataa tttttataat
    27061 gcttatactg ttttctgttt tatttggggt gatgtttata g
//
LOCUS       DAZABD010000039        26858 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000039 DAZABD010000000
VERSION     DAZABD010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26858
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.039"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..660
                     /locus_tag="ACHALG_001694"
     CDS             <1..660
                     /locus_tag="ACHALG_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFN2587085.1"
                     /translation="YISLLCKEEVRELPKSNSAVGIDLGIIDFAILSDGQKIDNNKFT
                     SKMEKKLKREQRKLSRRALLAKQKGINLFEARNYQTQKRKVARLHEKVMNQRTDFLNK
                     LSTEIIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSSFVSKLQYKADWYGRKIIKV
                     DKWFPSSQICSNCGHKDGKKSLEIREWTCPICHTHHDRDINASINILTEGLRLHSMGL
                     A"
     gene            822..1010
                     /locus_tag="ACHALG_001695"
                     /pseudo
     CDS             822..1010
                     /locus_tag="ACHALG_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
     gene            1287..3347
                     /locus_tag="ACHALG_001696"
     CDS             1287..3347
                     /locus_tag="ACHALG_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HFN2587086.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            3430..3999
                     /locus_tag="ACHALG_001697"
     CDS             3430..3999
                     /locus_tag="ACHALG_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN2587087.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(4174..4980)
                     /locus_tag="ACHALG_001698"
     CDS             complement(4174..4980)
                     /locus_tag="ACHALG_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587088.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            5118..6632
                     /locus_tag="ACHALG_001699"
     CDS             5118..6632
                     /locus_tag="ACHALG_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HFN2587089.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(7035..8018)
                     /locus_tag="ACHALG_001700"
     CDS             complement(7035..8018)
                     /locus_tag="ACHALG_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HFN2587090.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(8023..8670)
                     /locus_tag="ACHALG_001701"
     CDS             complement(8023..8670)
                     /locus_tag="ACHALG_001701"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding oxidoreductase"
                     /protein_id="HFN2587091.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(8755..9387)
                     /locus_tag="ACHALG_001702"
     CDS             complement(8755..9387)
                     /locus_tag="ACHALG_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFN2587092.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(9403..9954)
                     /gene="coaC"
                     /locus_tag="ACHALG_001703"
     CDS             complement(9403..9954)
                     /gene="coaC"
                     /locus_tag="ACHALG_001703"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HFN2587093.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(9951..10688)
                     /locus_tag="ACHALG_001704"
     CDS             complement(9951..10688)
                     /locus_tag="ACHALG_001704"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HFN2587094.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(10685..11674)
                     /locus_tag="ACHALG_001705"
     CDS             complement(10685..11674)
                     /locus_tag="ACHALG_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFN2587095.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            11932..12474
                     /locus_tag="ACHALG_001706"
     CDS             11932..12474
                     /locus_tag="ACHALG_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFN2587096.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            12491..13576
                     /locus_tag="ACHALG_001707"
     CDS             12491..13576
                     /locus_tag="ACHALG_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN2587097.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            13588..14394
                     /locus_tag="ACHALG_001708"
     CDS             13588..14394
                     /locus_tag="ACHALG_001708"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN2587098.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            14395..15222
                     /locus_tag="ACHALG_001709"
     CDS             14395..15222
                     /locus_tag="ACHALG_001709"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN2587099.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            15219..16292
                     /locus_tag="ACHALG_001710"
     CDS             15219..16292
                     /locus_tag="ACHALG_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN2587100.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            16720..19416
                     /locus_tag="ACHALG_001711"
     CDS             16720..19416
                     /locus_tag="ACHALG_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFN2587101.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            19481..19981
                     /locus_tag="ACHALG_001712"
     CDS             19481..19981
                     /locus_tag="ACHALG_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFN2587102.1"
                     /translation="MIKPMFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLS
                     SIQLLTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITK
                     TFLEKEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVN
                     EEEDFF"
     gene            19993..20487
                     /locus_tag="ACHALG_001713"
     CDS             19993..20487
                     /locus_tag="ACHALG_001713"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFN2587103.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            20508..21317
                     /locus_tag="ACHALG_001714"
     CDS             20508..21317
                     /locus_tag="ACHALG_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFN2587104.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            21314..22135
                     /locus_tag="ACHALG_001715"
     CDS             21314..22135
                     /locus_tag="ACHALG_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFN2587105.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            22186..24285
                     /locus_tag="ACHALG_001716"
     CDS             22186..24285
                     /locus_tag="ACHALG_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HFN2587106.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            24282..25400
                     /locus_tag="ACHALG_001717"
     CDS             24282..25400
                     /locus_tag="ACHALG_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFN2587107.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            25439..26317
                     /locus_tag="ACHALG_001718"
     CDS             25439..26317
                     /locus_tag="ACHALG_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFN2587108.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            26463..26660
                     /locus_tag="ACHALG_001719"
     CDS             26463..26660
                     /locus_tag="ACHALG_001719"
                     /inference="COORDINATES: protein motif:HMM:NF016526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFN2587109.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
     gene            26644..26858
                     /locus_tag="ACHALG_001720"
                     /pseudo
     CDS             26644..26858
                     /locus_tag="ACHALG_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320737.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         8553 a         4653 c         5630 g         8022 t
ORIGIN      
        1 tatatttctt tgttatgcaa agaagaagtt cgcgaactac ctaaatctaa ttctgctgtt
       61 ggtattgacc taggtattat agactttgca attctttctg atggacaaaa aattgataat
      121 aataaattta cgtccaaaat ggaaaagaaa ctcaaacgtg aacaacgtaa gttgtcaaga
      181 cgtgccttat tagctaaaca aaaaggtatc aatctgtttg aagctagaaa ttatcaaaca
      241 caaaaacgta aagtagcacg attgcatgaa aaagtaatga atcaacgtac tgattttctg
      301 aataagttga gtacagaaat tatcaaaaat cacgatatca tctgtattga agacttaaac
      361 acaaaaggca tgttgcgtaa tcataaacta gcaaaaagta tttcggatgt ttcttggtct
      421 agttttgtgt ctaaattaca atacaaggct gactggtacg gtcggaaaat catcaaagta
      481 gataagtggt ttccatctag ccaaatctgt tcgaattgtg gacataaaga cggcaagaaa
      541 tctctcgaaa ttcgagaatg gacttgccct atttgtcata ctcatcatga ccgagatatc
      601 aacgctagta tcaatatcct gaccgaaggt ctaaggctac actctatggg attggcttag
      661 atcaaataaa caagaaccgt aggaactacg gggatagctt ggtaaataag aggcacctct
      721 gttagtaaag taatacgcta tcaagtatgc tctcttccca agaagctccc acttcaagcg
      781 taagaaaccg ttaggtttag ctaagtggtg agtagttcac aaattacaat attgtgattg
      841 agagtcgaat cacttctcgt acgaatattt atttatctga ttgtttactt gaaaaattta
      901 ataaagaaca gataatttgg aaaaatccac cgactccaga tgactgggaa tattttggaa
      961 ataccatcat cagagaaaaa aatagacatt taactgaaaa aatcagctag tatttatgga
     1021 tggcagcttt gtctcacata tttattagct gatttatgtt tttttcaaag aaaaggtaac
     1081 tagatataac tagatataac tagatatatt acttaacaaa attaaaaagt acataagtta
     1141 ttattccgat tttcttgaaa caataaaaaa tcaaaaaaaa tactctccta atagtccatc
     1201 ttcttttgat tttatttata taatgaaatc atcttatgaa aattactact tcatgtttag
     1261 acaagagtag ggaggactaa gaataaatgt gttcaaaaag acgttcaaaa caactaaata
     1321 aacacgcttg tcgtttagat aaggaacggt cgtgttctga aaaacactac gttaagttta
     1381 tgatgcgcac gatgctgatc agtttatctg taggtgtcta tatgcatgta cccgttcagg
     1441 caacgacaca aaccatcaag gtcgtacgta cctctgtttc tgagaccact gattccccta
     1501 tcactattac tcatactttc actgatgcaa atggaaaaca gctacttcct gcattgactt
     1561 cctataataa agtcagttat cctccaacga taaaccacta ctggttaatt cctaacaaaa
     1621 cattatggac atcggaaacc ggaagcagtg tgccgctttc tatcttttcc gatatgactg
     1681 atcctacaaa aggtgatcag ttaaaacaga tcactgatat tttgaatcat gctttgacag
     1741 atataaagga cggtggaagc gcacaagttt cttatgtata tgaaaaagat tattcacagt
     1801 ttgaaagaaa gaaaatcgat gttctggcag ggacaaaagt ttcgatatct gatctggtag
     1861 aaaggctgaa gaacgcagat ggaacacaag gagatatgtc caaagtaatt tcaaacaatg
     1921 ggaatatcgt ggatacgact aatacaggaa catctactat ttccttgtcc tattttgatc
     1981 caattgcttt aaaaacaatg actgcatctg ctgtgatcca tgttcgtgct gatcaaacag
     2041 ctatcaaagg aaaagatctt cacttaagaa caggagagag ttacgcccct aaagatcttg
     2101 ttgaatatgt aacaaaaagc gatggaactt caggaaataa agaagaaatc aaaatgaacg
     2161 ggacagtaga tactacaact ccgggagaat atcccgttgt attaacttat atagactcga
     2221 aaacatcaaa tgtggcagaa acaacagctg tagtttatgt agaagcatct gctgcttcat
     2281 cgactgtcat cactcatttt atagatagtc aaacaaataa agaacttcaa gcagaaacac
     2341 atatgagtag tgtaaatcgt attcctgatt tgcgcctacc tgctgaattt tctgatcgag
     2401 tgccaaatat agatttatcg acttatgaac aaaatgggaa agtcacttct atttctgatt
     2461 ggatgcaagc aaacggatta gacagaaaaa gtcactggtc ggagatcctt gaagtacttc
     2521 actcagaact gatgaataat aggaagcaat caggagggga aattaccttt acttatgttt
     2581 atataccaga caatcgttca ttagaaggtc atgatcttat tgttcatcca aacgaaaaaa
     2641 taaacaaaga agagttgatc caatcagcaa caaattcctt aggtaaaccc gtaccgttaa
     2701 aggatgtatc ggttacaatc aatgaagtac cattggagga tggttatttg accaaacaaa
     2761 caggtgattt tacgattatg tatagttatc atgacccgta ttcactccaa gaactccatg
     2821 ctcagtcact attacatgtt ttaccattaa acaaacagat agatcaaaaa gaacaaaaaa
     2881 gacaagaaga aagcttgtca gaaaaaaaac aacaaatggc ccatgcagtt tctccaacag
     2941 ggaaagtaaa acaaacaaga agcctagagt tagcaagaat atctagaaaa atctcagaat
     3001 ctaataaaac gataaggcaa gaagctcatt tggccccaca agtttctgga ttggaaaatg
     3061 aaattttaga gcctaaaata aaacaacaat cagaaaaaga atttgttgct aaacagtcgg
     3121 aagatcagaa ggaatcagat gaccagaagg aaacgaagaa aaaacgcttg tcaactattg
     3181 attccaccga tgagcatctg cagccgcaag taccacttcc tagtccggga acacccgcac
     3241 ctggtggagg tattccttct tctgctttag gagatttcat gcgaggtatg gcaggaacat
     3301 gggtatacgc taatcgaaat gaatttgacg cagatgactg gttctgattt atatctctat
     3361 ctgttatagt caaataagta gctgagtcat atgttatttg gcataatgga gacgaggagg
     3421 gatagaaaaa tgttagataa aacgttacct tacgcagaaa tctggatgac aagaccactt
     3481 caacaaaatg ttccaatcac gcctttagcg gaaggttttg aatttcgatt ttatcaaaaa
     3541 gaagatgaat ctgcttgggc ggagattgag acttccgttg gggaatttca aacgacagat
     3601 gaagcacttg agtattttca acaaaacttt gctccttatc ctgaagaact tgaaagaagg
     3661 atgttttttg tagagacaaa aaaaggagaa aaagtagcaa cctgtaccgc ttggcgaaaa
     3721 aaatcaagag agcaagtgta tccggttttt cattggttag cagtgaaacc agattaccaa
     3781 ggaaaagggc tcgcaaaagc attgactgcc agagtcttgc aagaatttcc taccctccat
     3841 acggacggac caatctatct tcacacacag acatggagcc atcaggcgat tgctctttat
     3901 aaaaaaatgg gttttacttt tattgctgaa aatctagacg gcacacctaa tccagattat
     3961 gaaaaagtta tgcaggtttt aagtaaatta gataaataac gaaatggtga aataagtcct
     4021 cagtattggg ataaaaaacg aaatagtcat tcctcatatc ttactttttt gagttaatac
     4081 tatcggataa ctaatggcat gccatacatt caaaaaagaa ggggcagtga caaaagtatt
     4141 gtcactgccc cttctcttgt tttttagcat gttttatcgt ttgatttttt taggaataaa
     4201 attcttgatg aacgtaaatt tttggtaatc gtttcgacta tagaggtaac caaatacact
     4261 gaaaacaact gctccaataa aattaacaaa taaatccttc atagtatcca tgattcccac
     4321 gtctaagtaa ccattttgga tgattgtttt atctgtttta ccatctgcat tttgactttc
     4381 aatgattgtc cgagtgatat ctggaacatg atagacagtg tttccgtctt tacttaactc
     4441 cacactattg atttgctgga tgatcgtatc tttttgcata tcgaattgca gtaatttatc
     4501 tgctgaaaat tccacaaact cccacataac accaactgtc atcgaaaaac agaaggaaac
     4561 aactgctagt aataaaggag tcaagttcat ggtttttgta tttttattga gcaaataaac
     4621 aagggaaaag ccgacacccc cagctaaaaa tccgctgaat gcatgtagaa aagtatccca
     4681 cattgaaaaa tagccataaa aatcacttaa ctctcctaaa actgttgaac taaaaataaa
     4741 agataacaaa atgatttcta gcatatttgg aatctgtatc tttaatatct tttccagaaa
     4801 ataagggatt tcaaataaaa ttaatgtcag taataaaagc attgtatttc cccagtgttg
     4861 gttccagacc tgatccacta gcataataac gacgatgata cgtaataaaa tatagattcc
     4921 gtttgtgatt ctacctgcag tttttgtact gttcgtaaaa aaggttttca aatgattcat
     4981 aaaataactc cttcatgtat atccttaatt atactaagaa gcgtgtgttt accaaagcta
     5041 tatgacttaa agaaaaacag agttgatatt gtgaaaaaaa tcacttaata tagtataagg
     5101 agaaaagggg gatacaaatg aaaaaattag ttgctggttt attaacagcc gttgcacttc
     5161 ttgctatgag cgcatgcggc gcagatcaga aaaatacagc gcagacaagt tctagtacga
     5221 aggaatctgc cgaagtatca tcaggcgctt ctgaacaagt atatacagat ccaaaagaaa
     5281 tgaaagaatc ttatgatgtc gtgattattg gagctggtgg tgcagggatg gcagcagcta
     5341 tcgaagcaaa agatgctggg ttgaatccag taattttgga aaagatgcct gttgccggag
     5401 gaaacacatc taaatcatca agcggtatga atgcttcaga aacaaaattt caaaaagaag
     5461 aaggaatcaa agactcgaat gatgccttct atgaagaaac gctaaaaggt ggtggaggaa
     5521 cgaatgataa agaaatgctt cgatactttg tcgatcactc tgctgatgcg gtcgattggc
     5581 tagatcaaaa cggtatcaaa ctggacaact taacgatcac tggagggatg agtgaaaaga
     5641 ggacacatcg tccgtctgat ggctcggcta ttggtggata cttagtggag gggttattga
     5701 ataacgtcca tgagcgggaa atccctattt ttgtcaacgc agatgtgaca gacattaaga
     5761 aaaatgggga aacggtcaat gaagtgactg tacatgttca aggagaagaa ccaaaagagg
     5821 tcaccggaaa agcaatcgta gttaccacag gcggttttgg tgccagcaaa gaatttattg
     5881 aaaaatatcg tcccgattta gctgattata ttacgacaaa tcaagaagga tcaactggag
     5941 atggtatcac catgattgaa aaattaggtg gacaaaccgt tgatatggat aaaatacaaa
     6001 ttcatccaac tgtgcagcag gatgaggggg tcttgattgg tgaggttgtt cgcggagaag
     6061 gagcaatctt ggtcgatcaa gaggggaaaa gatttgtcaa tgaaatggat acaagggata
     6121 aagtatctgc agcaattaca gcattgcctg aaaaatcagc ttatttgatt tttgaccaag
     6181 gcgtgcgtga tcgtgccaca gctattgaat tttatgacca aaaaggatat gtcacaactg
     6241 gaaagacaat ggaagaatta gcagaaaaaa tcgatttacc aaaagaaaca ctagcaaaaa
     6301 cagttgatgg atggaatacg gatgttgcgg agaaaaaaga tgctcaattc aaccgtgcca
     6361 cagccatgga acatgactta tcgtctccga attattatgc gatcaaaatc gcaccaggaa
     6421 tccattacac gatgggcggt gtgaaaatca atacgaacac tgaagtatta gataaagaaa
     6481 accagccaat tagggggtta tatgctgctg gtgaagtcac aggtggctta catggaaaca
     6541 accggatcgg tggaaattca gttgcggaga tcattatttt tgggcgacaa gcgggcatac
     6601 aagcagctcg atttgcttcg gaaaatgaat gaaatagaac gtaaataaaa agaggttgtg
     6661 aaatcagcgt tttgagctaa taccgagggt cagctgattg aacactgttt agtgaagagg
     6721 ttgtgaaaaa gctgccctgc atcaagtaat aagaacagcc aagtaaaaac agcttcttat
     6781 gtttttaact tggttggact tattaccgca gatgcaagct tttgaacacc gtttattcgg
     6841 aaaaagagcg tgggacaaaa gtaaagaaca cttttgtctc acgctctaaa cacgtataaa
     6901 cggcggaagc agaagaaatt ccttcggaaa taagctgaaa ttcacaaaaa tttgaaaagc
     6961 aattttcgtg aatttcttct tatttcttgg agttaaacgc ttctgtccca gcctcttttt
     7021 tgatcagcat gattttattc agttatcttc aaaatgattt ttccgatgtt tttgtttgct
     7081 tccatatatt catgcgcttg tttgactgcg ctgaattgga atgtacggtc aatgattggc
     7141 cgaatcttgc cattttctag aaaaggccca gctttttgca taaaagtttg tgcgagctct
     7201 gctttatact gatcacttct aggtgtcaat aatgtaccaa gcagttggac acgtttccca
     7261 atcaatacac caaggtctat ctcaggcacg atggtcccac ctagcatacc aatcaagacc
     7321 caacgtccat caacggctat agaagctaaa ttcttttccc agtaagaagc gccgatgaaa
     7381 tccaaaataa catcgactcc ccgaccttca gtgatttttc gtacctcttc ttgaaagtct
     7441 tgttttttat agttgatcaa ttcatctgca ccaagttctt tacaaaaggc gagtttttct
     7501 tctgatcctg cagtaacgat gattttcgcg tgagaaagct gtctagctaa ttggatcgct
     7561 gctgttccca caccacttgc tcctgcatgg atcaagacag tttcgccttc ttgtaatttt
     7621 ccaagccagt aaagtgtttg ataagcagtc aaaaaaactt ctgagattcc agcagcagaa
     7681 acatagtcta gcgtatcagg gagaaggatt gcacgatcgg caggcatcac agcatattcg
     7741 gcataaccac ctagattgac taatccgtat acttttgtcc caggagtaaa agtggagata
     7801 tcacttttat tctcaactac ctctccagct acttcgactc ccaaaatagg gtagggaggt
     7861 ttcaaagaga ctgattctct tttcataata tctgttcgat tgacagctgc tgtatggact
     7921 ttgatcagta attcgccttg ttttggttta ggaacaggca cttcaatttg ttctaataca
     7981 tctacagaac caggatgctt gattgaaatg gcacgcatgg atctattcct cctttgccca
     8041 ttcttggaat gctgtattca actctttgct accttcagaa agtactagag tcgtacgaga
     8101 aatatccgtt tcttccacta gagcggcaag tacagcagca actgtagagc gagtgatcgt
     8161 ttttgatgcc ttctcattag gcttagttaa ttgaatggaa gtaacttctt catcgttagt
     8221 caatgtgact ggttgaagaa tgacaaaatc taagttagtt cgtttcagcc attcatctgc
     8281 ataatgttta gtaatataat aatctgtcat ggactcaggc cattttgctg gttcatctgc
     8341 ataaacagca ctgaccatca gatagcgaga gacatgcgca ttttcagctg cgatcactgt
     8401 tttgattgcg ccatccaagt ctacttgcag aagattcttt ccttgagagc cggcagcaaa
     8461 aatcaataca tcgatgcctt tgaatgcttc ggtcattttt tccacggacc aagttaaatc
     8521 aaaagagaca tacgaaacat tctctttttt tactaatgtt tgggaagcaa catctctcac
     8581 ccctgcaaaa atttcatggg aacttggagc taaatctttt atcaggtgtc ggccaatttg
     8641 tccgtttgca cctacaacaa aaattttcat ttggatcatc ctttcgacgt caaatctttc
     8701 cattcaatag taacaccaaa agagaaaaaa atcagagatc ggctacgatg actatcagtt
     8761 agtaagagaa gggtgcttga agtatcttaa caagacattg catactgctg cagtcgcaag
     8821 acctgcgacg attccttcag gtataccact tgtaaaaata accgctagaa tagctgaata
     8881 aacagcattt aaatttgttc cgatgacttt ggcataatct tgctggaata aaaagtagat
     8941 catattcatc actaaaatcg tattggtcaa tgaaccggaa agtccagcta agaataaaga
     9001 aacggaatct gctttttgtt tcattagttt gcgcagacct ttataaacga aataaggaac
     9061 caccccaatc aagatgcgag gaataaacgc gatcagcaag gctttccagc ttccatgttc
     9121 tgttccgatg actgggataa acggagaaaa gacaaaagac aaaggtgttt ggataactgt
     9181 gctacggatc atgctgatca gtccaaatgt accgccaaga aaagcaccta gtcttggtcc
     9241 taaaacgatc gaagcaatga ttacaggaat atgcatggtt gttgcattga ttggaccgat
     9301 agggataaaa cctaaaggcg taacagctaa taaaatcaaa atagctaaaa acatcgcagt
     9361 taatgtaaag ttttttgtat ttttcataag tactcctttt ttctaagcat ttctttcatt
     9421 aagagtcagc atgacctgtt cgatgatcgt ctgaatatca gctaaggcac cttttccaaa
     9481 atctccacaa gctaaaagag attctcttgg ttcaatttcc tgatagccga cagatctcaa
     9541 tgtttccaga ttacgctgag tgatcgggtt ttgatacata ttcgtgttca tggcaggggc
     9601 aatcaatttt ggtgttccca ctggaagcgc cattgcgact gttgaaagaa gatcgtctgc
     9661 aatgccgttt gctagcttgc cgataatgtt tgctgtagca ggcgcgatga gaaacaagtc
     9721 agcttgtttt gctagttcga tatgattgat tacctgcgga tttgattctt gcatgacgtc
     9781 tgtatggact gcgcgcttcg acagagattg caaggttaat ggcgtgataa aatgtgtgct
     9841 gcttttagac ataatcacat ctacctgggc acctaatttg acgaattgac ttgtaagatc
     9901 agcgcttttg taagcagaaa tacttccagt aacgcctagt aagatctttt tcattttgat
     9961 tcctccagtc taattttctc agtaatcaat gcagcaatct cagatttact ttgagcttct
    10021 tccactgttc catctttaga taagagatag ccgtggtgtg tctcgccatg gatttccgtc
    10081 aaatcatttg cgaaaataaa atcagttttg ttatttatca gactattttt tgcgactgcc
    10141 agaagagatt cctttggtac atctactaat agtttgaaac caatgatttt tgcttttggc
    10201 tgccattcac gtagataaga aatgattttt ggtgttttct tcaaaacgag cacaagatgc
    10261 tcggtgttag aagagatctt tgaaacagtg gtatctgtct gttcattttc cgcaaaccaa
    10321 ttgtccagtt cttcttgcgt tgaagaagtg ggtaggtttt ttgccagctg ttcttctgaa
    10381 aaactaaaag caggagtaaa atcactgact gccatactgt ggataatcgc atcgtagtgt
    10441 tcttttttcg ttagttcttc taattgtaaa aataagtcca gtgcagattc gatcgtataa
    10501 atcgttatat tggagtgtct tttcggtttc agtgcttgtt tcgtagtcac gtaatcgatt
    10561 gtggtatccg gattggcagc taaataatca gctaatgctt gccctaatcg tcctgtcgaa
    10621 tgatttgtaa tagcacgtac ttgatcgatt ttttcagtcg ttcctccagc tgtgattaga
    10681 atcttcattt atggtcacct acatttacta gtgttcttcc ttggtgtgtt ccagcaagta
    10741 gtttagatgc tgtttcaggc aattgatcca gagtaatctc ttggacgacc agttcatctg
    10801 caatctgcaa atccgccatc tgttgccata aagaaagacg ttgatctatt ggtacattga
    10861 cagaatcaat tccaatcaac tggatgtttc gcagaataaa aggcaagaca gtcgcgttta
    10921 gtttgattcc cccagcattg ccacataaag ctactgcacc gttataagag atcaatggaa
    10981 gaagactgct tagctgctcg ccacctactg tgtcgatgac atagtcgatt tgctgttttc
    11041 ctaaagcttt tgttgtctct ggcagaaatt catcaggtcg tacgacttgc aaagcaccta
    11101 gactttttag ccatgtgaca gactcttctt ttctggaaaa ggcaatgatt gactggaatc
    11161 ctagtttatg taacagggct atcgctgtac tgcctacacc gccagaagca ccagtaacga
    11221 caactttttt atcggaggtc ataccttgac ggattaaagc gttcacacac aacatagcag
    11281 taaaccctgc tgtaccaagg atcatcgctt gacgcagcgt catatttttt ggtagaggaa
    11341 ctaaccagtc tcctggtact ttttgatatt ggctataacc gcctggatgg gtgacgccaa
    11401 gcccaaaacc agtagcaagt acaagatctt cttttgaaaa acgtggattg ttactttgga
    11461 cgacagttcc ggataaatca atccctggag tcatcggata tgttcgtatc acctgaccag
    11521 actcgctcat tgccaatgca tctttataat tgacatcaga ataggcaatt tttaccaaga
    11581 cttcattttc agccaactca gtaaatggca gatcactgat tgctgtatgg aaaacaggtt
    11641 cctttattac ctgaaaagat cgaaaagcgt tcattcgtga gacactcctt ttaaaatttt
    11701 cttttatcat accataaagc ctactctttt attcttgttc ttccgtaaat ttagacaaaa
    11761 attaggtgaa aaataaagaa tcaaacaact agaatttatt ccaaaagata aagaaatatt
    11821 cagcaaaaaa aattttacaa atggttgcct ttttcaataa atttcgtata ctaatacgag
    11881 tggtagtaaa caggaagttt actattagaa aacttcgatt cggaggaaat tatggaaatt
    11941 ggtgagaagt taagaaattt gcgcatccaa aagaatttga cacaagaaga actaggcgaa
    12001 cgcacagatt tgtcaaaagg gtatatttcc cagttggaac gagatttaag ttcaccatca
    12061 atggaaacat tcttttctat tcttgaagtc ttaggtgtga ctccagaaga attttttcat
    12121 caagaagcag cgaatctgca agtggtttat tcaaaagaag atcatacggt ttatttcgat
    12181 gaagaaaacg ggtatgaatt ggaatggctc gtggctgatt cgaatgaaaa agaaatggag
    12241 cctgtcttgc tgacttttga aggtgcagga gaatataaga cgtttgaacc ttcattatct
    12301 gaaacgttta tttttgtatt agaaggggaa ttagagttaa gcttaggaga gacggtctat
    12361 gtggcaaaag caggacagtc gatctattat caagcgacaa aacaacatca attaaggaat
    12421 catctcaaaa aacgaacaaa agtattgatt gttgcaacgc aatcatattt gtaatatagg
    12481 gggtaaaaac gtggggaaac caatcatttc atttgatcaa gtagtgaaac gctatgatga
    12541 tgaaacagta ttaaaaaaag taagttttga gatcgaacaa ggaaaatttt atacactgct
    12601 tggtccttcc ggctgtggta aaacaacgat cctacgtatt atagctggat tcaccgaagc
    12661 tacagaaggg gacatctatt ttgaagggaa acggttgaat gatttgccag ctaataagcg
    12721 acaagtaaat acagtcttcc aagattatgc actttttcct catatgaatg tttttgataa
    12781 cgttgctttt ggattgaaga tcaagaaaat gtccaaatca gatatcgaaa agaaggtaaa
    12841 agatgctttg cgtatggtcc agcttcctgg atacgaaaaa cgggagatta gtgaaatgtc
    12901 tggtggtcaa agacagcgtg tagcgattgc acgagcaatt gtaaatgaac cgaaagtatt
    12961 gctattagat gaaccgttat cggcgcttga tttgaaacta cgtacagata tgcagtatga
    13021 actgcgggat ctgcaacaac gattaggaat cacgtttatt tttgttactc atgaccaaga
    13081 agaagcgttg gcaatgagtg atgagatttt tgtcatgaat aaagggaaaa tcgtccaaag
    13141 tggtacaccc gtagatattt atgatgagcc aatcaatcat ttcgttgctg attttgttgg
    13201 ggagagcaat atcgtggacg gtgtaatgat cgaagataat ctagttgagt ttgttggtaa
    13261 gcaatttgaa tgtgtcgatg gcgggatgcg tctaaatgaa ccagttgaag ttgttttacg
    13321 cccagaggat ttgacgatca cgacaccaga taaaggaaaa ctagtcgtaa cggtcgatac
    13381 tcagctgttt cgtggtgtcc attatgaaat catctgttat gatgaacagc aaaatgaatg
    13441 gatggtccat tcaacgaaaa aagctaaaga aggaagcaaa gtaggattgg cctttgagcc
    13501 agaagatatc catgtcatgc gtttcaacga gtctgaagaa gaattcgatg cacgtcttga
    13561 cagctacgaa gaataggggg gacaagaatg aaaacaatgc gcaggatcta ttccgtccct
    13621 tatattttgt ggctcggttt attcgtgatc gcgcctgtat taatgatcat ttatcaatct
    13681 ttttttgata tgaatgggca attcacgctg aataattatc tatcttattt tacgtcgggg
    13741 acatcgcttt ctatgacgat caattctgta tggtatgcat ttttgatcac gtttttcacg
    13801 ctgatcatca gttacccgac tgcttatttt ttaacaaaac tgaagcacaa gcaattatgg
    13861 ctgatgctga ttattttgcc tacatgggtg aatctgctgc ttaaagctta cgcatttatt
    13921 gggattttca gtgtgcacgg cagcgtgaat catttctttg attttatcgg gatcgggccg
    13981 aaacagatat tattcacaga cttcagtttt ctgttcgtgg caacgtatat cgagattcca
    14041 tttatgatta tgccaatttt caatgcattg gaagagatga atccttcttt gatcagtgct
    14101 agtagagatt taggcgcgaa tagctttgag accttccgac gagtgatttt tccgttatca
    14161 ttaaatggtg tgaaaagtgg tgtccaagca gtatttattc cttctctttc tttattcatg
    14221 ctgactcgtc tgattggcgg aaatcgggtg atcactttag gaacggctat cgaacagcat
    14281 ttcttggtta cacaaaactg ggggatgggt tctacaatcg gtgtgatttt aatcattgct
    14341 atgttcatcg tgatgctcct gacaggcgag aagaaaaaag gaggtcgctc ataaatgaaa
    14401 aaatttaaat ggtcgtatct ttatttaggg atcgtctttt tattcttata tgcaccgatc
    14461 ttctatctga ttttttattc cttcaatgaa ggaggaacga tgaacagctt tacaggattt
    14521 acttgggaaa attatgaagc tgtttttgaa gatacacgtt tgatcattat cgtattgaat
    14581 acgttcatgc tggctttctt atctgcattg atcgccacga tcatcgggac atttggggct
    14641 atgggaatct attacacgaa aaaacgacgt gcacggacag cgttgttgag catgaataac
    14701 atcttattag tctctcctga tgttattatc ggtgctagtt tcttgatttt ctttacacta
    14761 ctaggattcg gattaggttt tcagagtgta ttgctttccc atattgcttt cagcattcca
    14821 atcgtcgtct tgatggtctt gccaaaattg caggaaatga acgattcaat ggtcgatgcg
    14881 gctagagacc ttggtgctaa taatttccaa gtagtcaaaa atatcatttt gccttatctt
    14941 tctccaggaa tcatagctgg atattttatg gcatttacct attctcttga tgattttgcg
    15001 gttacatttt tcgtcacagg aaatggcttt acgacgctct cagtagagat ctattctcgt
    15061 gcaagacaag gaatcagttt agaaatcaat gcgctaagtg cgttagtttt cttgttctcc
    15121 atgatattgg tcattggata ctactttatt agtaaagaaa accgtccaaa acggctgaag
    15181 aaaaacagaa gagtacgcga gggggtggca cggctgcgat gaaaaaatta caatctctgt
    15241 ttttaggtat tctcatcatt attgcggtgc tgctgcttgg tgttcgtcaa cttgaaaaaa
    15301 caagtggcat gagtggagca aaggtactta ccatctataa ctggggagat tatatcgatc
    15361 ctagtttgat cacgaaattt gaaaaagaat atggatacaa agtgaattat gaaacatttg
    15421 attctaatga agcgatgttc acaaaaatcc agcaaggtgg gacaaactac gacatcgcta
    15481 taccaagtga atatatgatc caaaaaatga tcaaagaaaa actagtactt ccattagatc
    15541 attcgaagat caaaggatta aataatattg atccacgatt tttagacttg gattttgatc
    15601 cagataatca gtattcgatt ccatattttt ggggaacttt agggatcgta tataacgata
    15661 aattctttga tgcaaatcaa attaagcatt gggatgattt gtggaagccg gaattaaaag
    15721 acagtttgat gctgattgac ggtgcacgag aagttatggg gctttctttg aatagtttag
    15781 gctattcttt gaatagtaag aatatggcac agctgacaga agcagcaaat aaattgaatc
    15841 atttaacacc aaatgtcaaa gcaatcgtgg cagatgagat caaaatgtat atgatcaatg
    15901 aagaagcctc cgtggcagtg acattttctg gcgaagctgc cgatatgatg tgggaaaatg
    15961 aacatttaca ttatgtgatc ccaccagaag gatccaatct ttggtttgat aatatcgtta
    16021 tgccaaaaac cgtgaaaaat aaagaaggtg cctatgattt catcaacttt atgctagaac
    16081 cggaaaatgc ggcacaaaat gcagagtata ttggttattc tacaccaaat gaagaagcaa
    16141 aagcgttatt gccaaaagac atttcgtcgg acgaacaatt ctatccatct gatgacacga
    16201 tctcccatct tgaagtctac aaagatctag gatcgaaata cctaggcatc tataatgacc
    16261 tattcttaga attcaagatg tataggagat aatggcaaaa ctcagtaaga atggggactt
    16321 atcaacgttc taaagagcta aaaatcgtcc tttttctatc tgagttttcc aatcttctac
    16381 caaaagcgga tgaaacgccg ctttttttgt gttattttat tttttctacc acgtttttct
    16441 accagagaaa agtggacaaa accattgtaa aatagggccc gagacgggct agtaaatcta
    16501 agattgaaaa agttacagaa ggataaagta attggaaact atgcaaatat acttaaccct
    16561 tctgtaatct attaataaaa ctaatatatg aatccagttc aatatttgta gctatgaaaa
    16621 aacataaaaa tcatattaga taaaaactaa attggtagat aaattatttt gtcagatatc
    16681 tgaggcaaat attcgactat ttgtgtttca aagtaaaaat tggaatacaa atatagaata
    16741 gacatattgc agccgatagt ttattatgct tttagacaca ttaaactgga ggaaaaaatg
    16801 aaaaagagcg ctaaagatcg agtttttgat gagctgatac gactaacagg gaatatgtct
    16861 tctgtcactg ccgagaaaat ttcgaagaat ctaggtatct ctcgtcagaa tgccagccat
    16921 tatttgacga gattagtgga agataaaaag gtcgagaaat taccgggaaa gcctgttttg
    16981 tggaagcctt tagatgaata tgcggttgta gataatacgg agcaaataaa cgaagccttt
    17041 cattctgtag ttggtcatga cggaagtctg agagaggtca ttcaaaaatg tatagccgct
    17101 gtcaaatatc cgcctaacgg actcagtgta ttaataaatg gagccacggg tgttggaaaa
    17161 agttttttag ccacaaaaat ttttgaatac gctatccatg agcaaattat tgaaaaagat
    17221 gcaccatttg ctatcttaaa ctgtgccgat tatgcggaca atcccgaact gctttcggca
    17281 acactgtttg gatacaagaa gggggccttt acgggggctg aaaaggatac cgatggttta
    17341 ttggcaacgg ccaacaacgg ctatcttttt ctcgatgagg tccatcgcct atcgaaagaa
    17401 aatcaagaaa agctctttct atttattgat actggaaact atcgtccagt cggtgaaaat
    17461 gtaaattggc attctgctaa ggttcgtttt attttcgcca cgacggaaaa aggtgaaaat
    17521 tatttattag acacctttga tcggcgtatt caaattagtg ttatgttgcc aacttttgaa
    17581 gatcgcccca ttcgagaacg attagagctg attcaattat tttttcaaaa tgaagcggat
    17641 gttttacaaa aagatattat tgtttcaaga gaagccttgt ctttaatgtt atctcatccc
    17701 tttagtggga atattggtaa gctgaagaat atcattaaaa tcagttgtgc ggatgtgtat
    17761 agtcgaacac aagaagaacc attaacgatt gggaaaaatg agatcttgat tcagttaaat
    17821 atgctggaat ccgaagtaga aagcttgccg attgctagtt tattgataaa gaaaaatcaa
    17881 aagataaaaa cggatcgaat caaaccgttt gattatgatt cgattttgga aaaaattact
    17941 cagctgataa atgaaggtaa tttcaatgaa aatttttctt cgataaagaa tgaagtacag
    18001 catttggtgc atcacattga taaggattct tttttatcaa tgagtagaaa attgatgatt
    18061 cagatgttta gaaaggtctg ggtatccgtt ttaggaaaaa cgtacggatt gacgacgagt
    18121 ggacctgttg cagaaattat tagcagatcc tatgtcattt ccccaagcta taagacagac
    18181 ttaaccggga taaatcgtac attagctgca aatctacctc ggacgactta cttatgtaat
    18241 cagttcttgg agcatttacc accgctctca gaaaaggacg agaactttct aagactgctt
    18301 attgtcgtga ctttatctga ttatgtagat gaatccatcg agctgaaggg cctgttgctt
    18361 gctcacggag atagtacggc tagcagcatt caagcagtag tgaatcaatt atgcggcaat
    18421 tatgtttttg aagcgttgga tatgccagta gagagtaatg tgtcagagat tgtcgaatcg
    18481 acaaggaatt tcgtgaaacg gcaagtgaat acagatagtc tagtgctgat cgtagatatg
    18541 ggctctttaa atcaaattta cacacagatc aagaacgatt taaaagggga gttacttctt
    18601 ctgaataatt tgacgacttc cattgcactt gatattggct tgaagatgac gatgaatgcg
    18661 ccatttaaac agattgcgga ggatgcagca gacaagtatt caatcaatgt tcaatacttt
    18721 gaaggctttt ctcaaagtag caacattatc atttcctgta tgtcgggttt ggggatttcg
    18781 gacaaattga aagatgtttt tcgtcatcat ttatcggatg aatccatcga agttttcacg
    18841 aaggactaca gagaactacg tgaattaatc gaccaaaatg atgaaactta ttttgacaag
    18901 acaaaattgg tcatcacgac ttcggatctt ccacgttcct tttcaattcc taatattaat
    18961 gtttatgaca tcttagacgg tgatggagca gccaatttgg agcatatttt gagtcctgaa
    19021 atgaagccgg cagaatttaa ccgcttgatt caggagctag taaaattctt ttcgttggaa
    19081 ggaattgcgg accgtttgaa ctttctgaac ccaacgatcg tgatgagtga agtggaaaca
    19141 gtcatttcta aatatgaaaa ttactatcat ttcactttaa atggccgtgt aaaactaaat
    19201 ctctatatgc atacggcatt gatgatggaa cgtttattct tggctcggac agaaaaatct
    19261 gaagaaccga attatgattt gaaaccagat gaagatgaat tttattatgt ttctagaagt
    19321 atttttcaat caatggagat gaaatataat tttaaagtca atgcttatga gctttcgctg
    19381 ctctatgaat tattaggacc gtatattcaa aaataaagtg tctaaaaaag tgtctaatct
    19441 gtttttttta ggttagacac tttttttaat gtgaattaac gtgattaaac caatgttttt
    19501 aaaagttggc acaggaattg cttatataaa agtgaacaga aagaaaggaa ttaaccatat
    19561 gaagaaaatt ctaatagcga ctcacggtca cttagcttca ggctttctta gcagtattca
    19621 attactgacg gggaaggttc aagagattac ggtgattaat gcttatgtgg atgagtgtga
    19681 ttttgaacag gagttgaatg tatttgtaaa ccaggtgaac gagcaggatc agatttttgt
    19741 ttttaccgat ttatttggcg gaagtgtcaa tcagaagatt accaaaacct ttttggagaa
    19801 agagattgct gtaactattt tgtctggctt taatctgcca attctgctcg aaatagtatt
    19861 ggcaaccaag gaattaacaa aggaggatat tcaggaactg gtaaataaat gccaacagga
    19921 attaaagatg accattcttg atgaggtaca tgtagtaaat gaagaggaag atttttttta
    19981 ggaggaagaa aaatgattaa agcagtgcga attgatgaac gattaattca tggacaagta
    20041 gcgatgactt ggacaaagag tctaaattta accgggttag tagtagcaag cgatgaggcg
    20101 gctaccaacg atattcaaaa gatgacattg aagatggctg caccatcgaa tgtcaaagtc
    20161 attattaaaa cagttgatga cgcgattcat ttgttaaaag atccgcgggc gaaggacatg
    20221 cgcttgatgg ttttagtccc taatgtcaaa gatgcggtaa aagttgcgaa agctttccca
    20281 acggaaatcg aattaatgaa cgtggggaat gctgggaaaa tgacaccgcc atcggaagat
    20341 aagaaaacct tatcaaagga agtgatgtta acgacgtcag aaattcaaga cttaaaagaa
    20401 ttagttgaac tttatccaga taccgttttt cagcctacac cggcgatgga aaaacgttca
    20461 gctatctcta ttttgagtga tttttagaaa aaggaaggag aaaaattatg tatgatgcat
    20521 ttgtagtcgc tttggccgtt tttattggag ttgctggaca tgaaatgttc gggatgtcca
    20581 tgctgagtcg tccgattgtt gttgctccgt tagcaggagc gttgatgggg gatttgcaga
    20641 cggggttgat tgtcggggcg tcactagaag ccatctttat gggtgtcgtc aatatcggaa
    20701 tctctagtac ggcggagcct tctttggctg ctgggctggc tacagcgttc gcaatcaaaa
    20761 tgggaggaaa cgtggatgcg attattccca ttgtctttcc actagcagta ttaggattac
    20821 agttaatgaa tatgattttt tcttttgtat gtggaccgat ggctgctaaa tttttcaaat
    20881 ttgcaaaatc tggtgatcaa aaaggaatca tcaatctgca cttctttatg tggtttgtcc
    20941 actatgcgct ttatgctctt attccattct ttgccgtttt atttggagca gatgttgtca
    21001 aaagcgtctt ggataatatt ccagaggtca tcatgaacgg attaacagtc gctggaaatc
    21061 tgctgccggc agttgggatg gcgatgctgt tacggatgct ttggagtaaa aatattgctg
    21121 tgtggttttt ctttggggcc gttgttatgg cgtatctcga gttaccattg attgcaattg
    21181 ccgcagttgg aacgattatt gcaattgtta tagcccaaag agatctacaa ctaaaaaata
    21241 tatctgttca tgcagttcaa agcgatgctg ctgtaaatgc aacgcttgac caagaagagg
    21301 aggatttctt tcaatgaaat tagctgatgg aatttctaaa gatgagaaaa aattggttcg
    21361 gaagatgttt tggcgctcga tgactatgta tgcttcggtg aatccggtga cgatgggcgg
    21421 cggtggtttc gcctattcta tgatgccctt cattaatcat ttctacaaaa gtgaagaaga
    21481 tcgagctgcg gcgctggaac gtcatacaca gtatttttcg acgaccattc cttgcgcgtc
    21541 ctttgtcatg gggattgcgg catcgatgga gaaggaaaat tctgaaaaag ctggttttga
    21601 cgtggaaagt atcaacgcga tcaaaacgag tttgatggga ccattggctg gaattggcga
    21661 ttcattgttc tggggagttt ggcgtgtcgt taccgcggga ctagcgatca acttagccaa
    21721 tgccggaaat attttggcac caattatctt tttagttatg ttcaatatac ctaattactt
    21781 gatgcgatac tatggcggcg ttttaggcta ttcattaggc tctaaatata ttgaaaagct
    21841 gtatgcgaac gggttaattg aaattctgac aaaagctgct agtatcttgg gtctgatcat
    21901 ggtaggtgca atgacgatca gtatggtcaa attcaatacc gttttgagct tttcaatgaa
    21961 gggcgaagaa ctaatgaatg tccaagaaat cctcgatagt atttttaaag gaattgtacc
    22021 gttattggta acattgagct gtttcaaatt actagataaa aaagtcggcg tcatcaaaat
    22081 tatttttgga atggtcgtct tgggaatttt attgtcatta cttggaatcg catagtagga
    22141 agctgcagct actaggctgc agttttttta gcaaggaggg aaaagatgac tattaatgaa
    22201 gtaatttatt cacccaaaca cccatttttt aatgctgagt taaacgacgg aaatgtgact
    22261 acttcaaacg aatacagcta ctatcggaat attcctgatg taaagggatt gaccttcgat
    22321 gtaagtaatc aaaagacatt agcaaacctt gatgtttgcg ggacgattca aacgttgacc
    22381 ttttatcgag aaaatttact aactgaggaa aaaccaggtg tttgggtcaa taaacagctc
    22441 tctcaaacca attcgttatc cttaaaggtt gaagtaaacg ggattgtcta ccatttgtca
    22501 gaatcggatc atcgtattga agtcgatgtg attcaagact gtttgcctag atatattcat
    22561 cattattcga cgtttaaggt cattgtagta tcctttgctc ccatactggc taataagcgt
    22621 ttaagtatgc ttgttcaaca ggtttacatt gtgaatgaaa cacacgaaag ccttcaagtt
    22681 tcagtgaaag atgtgccttc gtatcaagag aagtattcgg atcagcaaaa tgtgttgatt
    22741 tctcaaaaag gaaataagga cacgatcgca caacaggaag tggcaagctt cgccattgct
    22801 tttgtggacc caaatgcttt tgaagaagta atgacttttc aggaatctga tatcgaaaaa
    22861 tggctgaagg aaacattgaa ctattttgct cagctatttg ggcagctgtc aatgccaaac
    22921 gatagagttg tccatctcta tcgtcgtgca ctttatcaaa gcttttctag tttcggtatg
    22981 aatcgacagg gacaggtcgt cggcagcaat tggggcagct atcctgctac taatcgaatt
    23041 tggaataagg atatgtatta cgcatcattg ccattcacca tgtttgaacc ggagttatgt
    23101 caaaaaacgg ttctatggtt tgatcaatat ggcgttaagt ttcctggttc aaaatttaca
    23161 ggaggtatca atcattctct gagtaattcg ttatccagtg cgttactggc tagtctttat
    23221 tttgaacata ctggggatac aaaattcttt gagcggtgtc cgcaggttct actgaatgct
    23281 tcaagaatta ttgaggacat attagctcaa cgtcggtcag gggaaccaat gctttttcca
    23341 tctgtttggt tatcagacgc ctttgcactt ggaaagtatc ataccggcag caatctttgt
    23401 ctttggaagg cctgtattgg acttgcagaa atttttgaag ccctagaaca actgagtctt
    23461 agcaagcgat acaaaaacat tgcctgtaaa ctaaaggaat caatttgtaa acaaatgact
    23521 atcgatggtc cttttggtga gcaatttttg gagggaatcg gtgatgagga gaaaacaacc
    23581 tattcagtga agcactatca aaaaccgatt cttgagcaag ggttaatctt tttaagtgat
    23641 gtgattgtgg atggaaaaat taatttattg atgcatgatg gagaagaatc agacactacg
    23701 ttgatgcctt tttatcaatt tttagataat aaagatcatt tatatcagaa caccatggcc
    23761 ttttctgcta gttctttcaa tccaacctat agcgaggaaa tcaaaggcat cacttgggga
    23821 ttagaatccg gcgccacttt tcctggcttt atcactgttt taatgagcga gctgcacaac
    23881 cctcaagctt tcgtaacccg attagaagaa ctaatttgtt tggcagattt agacggttcg
    23941 tggtggtggt ggccgtatcg cttagaggct caacaaggag aagtcgtgcg agactttggc
    24001 tgcggcaaat gcggctgggc atcgggtctt tttgtcagcc taatgatcag tcaatatttc
    24061 ggattgaaat tcaaaaaagg agtattgcaa attgatcctg ttgataattt aacgttttct
    24121 tggaagaatt tgaagttcgg tcaagccttc atttcgattg aatgtacgca atccgagttg
    24181 agtatttcaa atttgtcaga aaagccattg aaaataagtg atacgaaaaa aattcttcac
    24241 gtagaaaagg gaaaaacgat tcacattcaa aggagaaaaa gatgagtata ctaaaaaata
    24301 ttacccaatt taaaaaaatc cagagtcgag caatcactcc cgaaaatcca acaggcgaga
    24361 aaggtaaagc tgcgatgttg ccaagcgagc taggaccaag ccgcaaaggt cgtggcgcaa
    24421 ttccgctgac ttctggtgaa gaaactgtga ttgcggatat tactggaacg ggaataattc
    24481 gtcatatgtg gatgacgatt cgtgatcgaa cagaatttgg cagttttgtt ttacgtgatt
    24541 taatcttaag aatttattgg gatgggtcgg atagccctgc tgttgaaagt cctttagggg
    24601 atttcttttg taatgggttt ggagagcgtt gcgaggtgaa ctcgttgccg atagtggtta
    24661 atcctacagg gggctttaat tcctatttcg aaatgccttt ccaaacatcc gctaaaatta
    24721 cgatcgagaa tttacatccg aaggatatgt cctcattttt ttatacgata aattacgctt
    24781 tggtggacga attggaagaa aatagcctat attttcacgc aaattggcat cgggaacgca
    24841 ttaccaaatt acaggaggat tataagttaa ttgatcgatt aaagggtcaa ggctattacg
    24901 tgggtactta ccttgcgctt acctgcctag aacgttattg gtggggcgaa ggcgagttta
    24961 aattctacat tgatggggat agtgagtatc cgaccgtaac ctcaacaggt tcagaagatt
    25021 attttggcgg ggcgtgggcc tttcatgagt acgatgagtt cggacgtcca catgcgaaaa
    25081 actattcaac agccttttta gggtatcctt accaatcaaa tagagatgcc acaagagatc
    25141 gttttgaaac gggaaaattg aatgccgttc atgcctttgg tgacgacgca atgccacgac
    25201 atggtcttta tcgttggcat cttcaagacc ccatctgttt taccgaagat ttatcggtca
    25261 ctttacaaca aatcggcaac gatgatgttc gcttatttga gcggcaagac gatgttgcta
    25321 gtgtcgctta ctggtattca acagaagctt caggaaatac taaacgtcat ttcactgatc
    25381 gagaacgctt gccgcgttaa tgaagtgttt ttcacatatt cgaagggagg aacctatgat
    25441 gatatatggg gcaattgaag ctggagggac taaatttgtc tgtgcagtcg gtactgagga
    25501 attaacgatt ttggaaagag tcagttttcc cacaacaact ccagttgaaa caatgaagca
    25561 ggtcattgat ttttttaaac cttttaagga acaattggca ggcatcgggg tcggctgctt
    25621 tggtccaatt gatattcaat gcaaatcaac gagtttcggt catatcacat caacaccaaa
    25681 acttgcttgg caaaatttca attttgtagg aacgctaaag caatatttca atattccgat
    25741 tgaatggaca acagatgtca atgcggcttg ctatggtgaa tatgttgctg gttctggcaa
    25801 agggttagca ggtgttgcat acttcactgt gggaacaggt atcggaggtg gtgccttgtt
    25861 caatgggact ttcgtggaag ggttcagtca tccggaaatg gggcatatgt tgatcaagcg
    25921 acatccaaag gacaattttt caggaaactg tcccttccac caagattgtt tagaggggat
    25981 ggcagcagga ccagcgattg aaaaacggtt gggtgtaaaa ggccagaatt tattggctga
    26041 cgattcgttt tggcagattg aagcctttta ccttgcccaa tgtgcataca atacaacact
    26101 gatgttttcg ccagatagaa ttatttttgg cggtggtgta atgaaacaag aacacatgaa
    26161 gaaaaaagtt caggataaat ttgttgagct tataaatgga tatgttgaaa taccaccaat
    26221 tgatagctac attattacgc ccgaattagg tgataatgcc ggaataatcg gcggattagc
    26281 attggccaga aaagcggtaa gaaataagca accataatta atgaatgttt cattgatact
    26341 catgtcaaaa gtatttaacc gagtaatcaa atgatcaaat gaagttggat aaagcgaaaa
    26401 tgaattcaaa taaaaaggct ctaagtcaaa tcgtgttgtt ttcattaaat cactgaaagg
    26461 aaatgcttct gcgtttcctt ttttcttttt gttgtgcgct gtataccttg aggatattca
    26521 ctggtggcgt acacttaaat agtaatatat tgtgttactt tatgtctgtg gtaatagtta
    26581 tagttgctta ccaatgtatg ttcatttttc gaaatgttga cttcataatc cttggcttaa
    26641 tgtttgaatt tttttggtga tagatttttt cagccgtata aaataaatcg taattctatt
    26701 atagagagtt gtgattataa aaagatagca agaaaaaaaa gaaagtttct atacatttta
    26761 aatattttat tcctatttct attgctaaaa aagtagaaat tctatattta cctttaactc
    26821 tcgcttctct tgcaaatgta gttagcattt taatcgat
//
LOCUS       DAZABD010000040        26547 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000040 DAZABD010000000
VERSION     DAZABD010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26547
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.040"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            283..522
                     /locus_tag="ACHALG_001721"
     CDS             283..522
                     /locus_tag="ACHALG_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587110.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            complement(884..1144)
                     /locus_tag="ACHALG_001722"
     CDS             complement(884..1144)
                     /locus_tag="ACHALG_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587111.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(1329..1826)
                     /locus_tag="ACHALG_001723"
     CDS             complement(1329..1826)
                     /locus_tag="ACHALG_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFN2587112.1"
                     /translation="MWTEKQKKDTYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(1956..2666)
                     /gene="budA"
                     /locus_tag="ACHALG_001724"
     CDS             complement(1956..2666)
                     /gene="budA"
                     /locus_tag="ACHALG_001724"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HFN2587113.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(2679..4343)
                     /gene="alsS"
                     /locus_tag="ACHALG_001725"
     CDS             complement(2679..4343)
                     /gene="alsS"
                     /locus_tag="ACHALG_001725"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0034077 - butanediol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HFN2587114.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(4548..5210)
                     /locus_tag="ACHALG_001726"
     CDS             complement(4548..5210)
                     /locus_tag="ACHALG_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFN2587115.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(5220..6035)
                     /locus_tag="ACHALG_001727"
     CDS             complement(5220..6035)
                     /locus_tag="ACHALG_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN2587116.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLYESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(6289..6732)
                     /locus_tag="ACHALG_001728"
     CDS             complement(6289..6732)
                     /locus_tag="ACHALG_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HFN2587117.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(6866..7204)
                     /locus_tag="ACHALG_001729"
     CDS             complement(6866..7204)
                     /locus_tag="ACHALG_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFN2587118.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(7192..7569)
                     /locus_tag="ACHALG_001730"
     CDS             complement(7192..7569)
                     /locus_tag="ACHALG_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFN2587119.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKEIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            7793..8986
                     /locus_tag="ACHALG_001731"
     CDS             7793..8986
                     /locus_tag="ACHALG_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFN2587120.1"
                     /translation="MTTYQRKVLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(9152..9532)
                     /locus_tag="ACHALG_001732"
     CDS             complement(9152..9532)
                     /locus_tag="ACHALG_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN2587121.1"
                     /translation="MIHLLLETERWCSIEELQNELKVSSKSILNYLTDLEELFQKYPD
                     KVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQHPEKNLEDYADAQY
                     TSVSTVFRYAKLLVKYFEMTDVKE"
     gene            9785..9979
                     /locus_tag="ACHALG_001733"
     CDS             9785..9979
                     /locus_tag="ACHALG_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587122.1"
                     /translation="MVAGSLLSGCGATTSTTQSTSSDQASSSSTTISESTADSSKEQE
                     QIEKKKEEKQKAEKAALEKK"
     gene            9976..10470
                     /locus_tag="ACHALG_001734"
     CDS             9976..10470
                     /locus_tag="ACHALG_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587123.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQSA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
     gene            complement(10632..11804)
                     /locus_tag="ACHALG_001735"
     CDS             complement(10632..11804)
                     /locus_tag="ACHALG_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFN2587124.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(12010..12825)
                     /locus_tag="ACHALG_001736"
     CDS             complement(12010..12825)
                     /locus_tag="ACHALG_001736"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFN2587125.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            complement(12975..13421)
                     /locus_tag="ACHALG_001737"
     CDS             complement(12975..13421)
                     /locus_tag="ACHALG_001737"
                     /inference="COORDINATES: protein motif:HMM:NF027869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFN2587126.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            complement(13418..13933)
                     /locus_tag="ACHALG_001738"
     CDS             complement(13418..13933)
                     /locus_tag="ACHALG_001738"
                     /inference="COORDINATES: protein motif:HMM:NF037639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
                     /protein_id="HFN2587127.1"
                     /translation="MDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFT
                     SSKDGTFSVHGLSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEP
                     KANPTNNGKTPVQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKK
                     QQKGNNRREIK"
     gene            complement(14006..14920)
                     /locus_tag="ACHALG_001739"
                     /pseudo
     CDS             complement(14006..14920)
                     /locus_tag="ACHALG_001739"
                     /inference="COORDINATES: protein motif:HMM:NF027869.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
     gene            complement(14917..17244)
                     /locus_tag="ACHALG_001740"
     CDS             complement(14917..17244)
                     /locus_tag="ACHALG_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBG domain-containing protein"
                     /protein_id="HFN2587128.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            17853..17999
                     /locus_tag="ACHALG_001741"
                     /pseudo
     CDS             17853..17999
                     /locus_tag="ACHALG_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332517.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
     gene            complement(18087..18380)
                     /locus_tag="ACHALG_001742"
     CDS             complement(18087..18380)
                     /locus_tag="ACHALG_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HFN2587129.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            18417..18602
                     /locus_tag="ACHALG_001743"
     CDS             18417..18602
                     /locus_tag="ACHALG_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587130.1"
                     /translation="MLEYSGFFIKAVTLKKDIIIVLFFLLLLIGRFILIHYSPLPIIK
                     EKTGSGCSNYMLRKKNP"
     gene            complement(18783..19961)
                     /locus_tag="ACHALG_001744"
     CDS             complement(18783..19961)
                     /locus_tag="ACHALG_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN2587131.1"
                     /translation="MENMSRFKKHITATGKITWYFQAFRTTRRGFNSKREAQLAYLEM
                     EKQHRNNQKRIASNDKFSVVSQKWFEYYRSLNEQKVATYDKREEQVKILNRWIGDEKL
                     SSLSSEELQKFLFILKEKGIDGVDVGYAKNSLQSLVQVLNMVFNYCKKKNLINENPMK
                     FVKMPKYQLTVKDLKESVNSLEDKFLTVDELRTFLNYGIIYEELPMSVLFHVLFYTGC
                     RVSEALALQLQDIDFGRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVPVTPLVMEK
                     LHELIEVLNKTKRNSNFVVDETYLFVYLDPGKCGVPYRREYVNDHVKRCVERCGINKE
                     FHTHLARHTMASLVAEYCSWDVLKDRLGHTDSTTSKIYRHLTSTEKLKPLAAFSSLED
                     "
     gene            complement(20064..20375)
                     /locus_tag="ACHALG_001745"
     CDS             complement(20064..20375)
                     /locus_tag="ACHALG_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN2587132.1"
                     /translation="MLKNNNKSNTSELENYLFYLESIEDSINKIKRINELPLVLTNKD
                     LKEQFQISDSTLNRLIKLDNFPRCWFGIRGHYFREDIMEWVKKGKEEYFCDQFRLLRI
                     L"
     gene            complement(20731..20976)
                     /locus_tag="ACHALG_001746"
     CDS             complement(20731..20976)
                     /locus_tag="ACHALG_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN2587133.1"
                     /translation="MVNKQKKRQYLLVPLSIILSALEEDPISIKMILKHYHSYIAKLC
                     LTNGFNEVGQFITYVDEYMLRQLENKLIEAILKFKIN"
     gene            complement(20970..21368)
                     /locus_tag="ACHALG_001747"
     CDS             complement(20970..21368)
                     /locus_tag="ACHALG_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFN2587134.1"
                     /translation="MQKKTEKSIQHQFDAYCKKVLRNEAKSIRKRNIRTKVNEEPLTY
                     LNEGEYSQTHSDEQDAYFLFGMEILISDQKLSAAIDQLSDIRRKIILLYYFAGFNDAE
                     IGKILNMSTSGIWYQRTKAVEQLKMRYDLW"
     gene            complement(23483..23950)
                     /gene="tnpA"
                     /locus_tag="ACHALG_001748"
                     /pseudo
     CDS             complement(23483..23950)
                     /gene="tnpA"
                     /locus_tag="ACHALG_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002458050.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(24699..25229)
                     /locus_tag="ACHALG_001749"
     CDS             complement(24699..25229)
                     /locus_tag="ACHALG_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFN2587135.1"
                     /translation="MNNKKSPVYDIKSVPIEKVFPNDYNPNSVAPPEMKLLYDSIKAD
                     GYTMPIVCVYDDSEDKYIIVDGFHRYRVMLEYEDIFDRERGCLPVSIIDKPIDQRMAS
                     TIRHNRARGTHDVDLMSNIIKDLHELGRSDNWISKHLGMDPDEILRLKQITGLASLFK
                     DHDFSEAWEPDEQLIE"
     gene            complement(25309..>26547)
                     /locus_tag="ACHALG_001750"
     CDS             complement(25309..>26547)
                     /locus_tag="ACHALG_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009269766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HFN2587136.1"
                     /translation="YVSFSGGKDSGLLLQLILDFRDTYYPSRVVGVFHQDFEAQYSAT
                     TKYVEETFTSLSSNLIERYWVCLPMATRTALSSYEMYWFPWDDKKKESWVRPMPQHEY
                     VINMDNNIMTTYRYKMHQEDLAKQFGRWYKQSHGDKKTICLLGIRADESLQRYSGFIN
                     KKYGYKNQCWITRQFKDLWAASPLYDWTTEDVWHAYFKFGFKYNELYNLYYKAGLNIS
                     QMRVASPFQDYAKDSLNLYRVIDPGIWVKLVGRVRGANFASIYGKTKAMGYRTVSLPN
                     GHTWKSYTQFLLCTLPKELRDSYIIKFNKSINFWHNIGGGLEEETIKELKEHGYKIKR
                     NGISNYTIFKNSRIIFLQKIPDDTDDIKTTKDVPSWKRMCFCILKNDHICRFMGFGLT
                     RQQQKRLDYIKNKYKDINDL"
BASE COUNT         8020 a         5097 c         4233 g         9197 t
ORIGIN      
        1 ctatcctcct agcaactatt taaataccca aaaaaattac ctctagataa tatcatacct
       61 acgttttcac ttataaattt tttcataaaa aaagcgttta gaggcataat cgttaataca
      121 gatggtaacc aatgtatctg gtttgaactg ggatatcacc ctctccatat atgtattact
      181 aagactaata gatactataa aagacttgat agttttgcat tgattttgcc taatactgag
      241 cataggtgta tgctaaacga tactataagg agtgtgtaca taatgaaaag aattctgttt
      301 atcagtttat caaatttatt gttacttact gcttgtggga cttctgcatc aaagaacata
      361 ccttcagata cgcaaatttc gaatagtatc gattcaacaa tttctacaaa tgagaaaaat
      421 tctccggtag aaacttccga tataaaagtt tctacctctg caaaaaacag ggtaaagaaa
      481 ctgtcagcac gtctccttta tttcaaacaa actctgaatt agcttcttca tcttcttcag
      541 caaataatga tgatagtttc gcgtttactc ctaactaaat gcaaccttta caaatgcttt
      601 acgataacaa gcctgagcta aatgatcctg atatcagttt tgcatttttt gaaatgattg
      661 gatcggatga tctttttaaa actactagtt tgtctatccg aaaataggac agttctggca
      721 gtataggttt ttatcgagtt tctccaccag gcctcgttac agagacagat tctctaggcg
      781 aaccttttga ataaatcaaa aatcatgaac aatcactgcc aactaaataa agtgccttca
      841 aaagcttggt cttctcgctt ttgaaggcac tttatttaaa tctttatgaa actaaagtat
      901 gttcatattt gttgctatag ttatatagat attctgttgt ttccaactca taaacgtgag
      961 tatattttgc cactactttt ccgcattttt ctaatatttc attgcgatcg acgtcatgat
     1021 attcgtctac gcagatcaca acacaatttt ctaatttact gattacttct ccaaccactt
     1081 tttgtttcaa gccaataggt tggcaaatat atttttttcc taaaatgata gtttcgttat
     1141 tcatatttca tccatcctta atgtttttcc attatttaaa atgataatac cattaaaata
     1201 tgattatgaa aatcaataac acttgtgatt ttataaaaaa ataaaaaaac cgaaaaacag
     1261 aaatgcccac tgtctttccg gctcttttta ttcttgattt ctcttctttt ttgtttcaat
     1321 agacgatttt agttattaat aaatgaaaaa gcatttatat taaaatttgc taataaaaga
     1381 tctacatatt tttctagaaa tgcttgatcg atttcatcat accctttcgt tactccgcaa
     1441 tcgatatcta aaacaccgat taaattacct tcttttacca ttggcactac gatttctgat
     1501 tgtgcggctg aatcgcagga aatgtaattt gcatgggttt tgacatcatc aacgatcaat
     1561 gtttcttgtt tttcagctga ttctccacaa actccttttc ccatcttgat acgtgtgcaa
     1621 gaaactcgcc cttggaacgg tcctaaaatc aattcattac catcgaataa ataatatcct
     1681 gcaaagacag tctcaggcaa agtctcattc aacaatgcag atgaatttgc taaatttgct
     1741 aaccagttat cttctgcttc taataatcct tgttgctgta ataataaaat ttcgtatgta
     1801 tcttttttct gtttttctgt ccacatgata aaaaccgctc ctttttgatc tatggagaag
     1861 attatagctc agtttttttg taaacgctag gtttcctcct caaaaaaacg agatacgtca
     1921 ggttaatcac cttgaacgca tctcgctgtc tttttctatt ctgcttgttg gatttctgct
     1981 gctaaatcag cataatcaat cgtttcgtga acaaaagtcg gatcatcaac tggaaaatgc
     2041 tgtacaaggg acgagatgcg gctaatctca acttttcctt cagccacttc aaaatccatt
     2101 acatgtccgc caaatgtatg tgtatcatcg ataaaatgca agtgaaatcc tgctgcagca
     2161 actccttgga ataattcagg cgtaaagaac ccgactaatg ttccttggac attgctttgt
     2221 tggaattctg gttgagactc tgaaattttt gctagtcttc tgtaaggttt ttcttgcttc
     2281 ggcatgatac ggatgtgcat atttttgaat aatccagtta tcttcactgc caaaaatagg
     2341 ttttccccgg cttctcttac taatatgtct tttttcaatt gttctccatc tgcagttttt
     2401 gagacagaaa aactagtatc tggcgtaaaa tctgtaattg ctgcataggg ggtaagttca
     2461 cttccatcta attgggcaac tgagccatct gatcttcctt gataagctac gccatctaaa
     2521 aagatgactt caccatcaag cccatgcaat gttcccaatc ccagagttcc tttttctaat
     2581 atatgagcaa tcgtttctgt tccatccatc aagccagcca tcaaagctcc taatgtcccg
     2641 tgctggtata agattttttc agtcatgtcg acacttcctt aatagaattg atctggtaag
     2701 attgtttctc ccaatttctc attatcacgg taatcaatcg gaatatcaat aattacgggt
     2761 ccttctgtcg caaatccttc ttctaaagct ttttctaatt cctctacact tgtagctcgg
     2821 attcctttag cgccaaaagc ttctgcatat ttcacaaaat caactggacc aaagtctaca
     2881 cctgacgccc gctgatattt cattttttct tggaactcta ccatattgta gtgtccatca
     2941 ttccaaatca aatgaacgat attcaatttt ttacgtaccg ccgtttctaa atcctgggca
     3001 gagaataaaa atcccccatc gccggaaact gatacgattt gtgtatttgg acgcaccaat
     3061 gcagcagaaa tcgcccatgg caatgcaaca ccaagcgttt gcataccatt actgaataac
     3121 aaatgacgag gttcataact tctaaagtgg cgtgccatcc aaatataatg actacctaca
     3181 tctacggtaa cagtcatatc atctgttact tttgactgaa gcgtgttaat aacttctaaa
     3241 ggatgcaaga tacctgcttc tccttttgat tgaacaatgt cccgctccgt taattctgct
     3301 tgaagagatg ctaaatattc ttttgcatct tctgacactt gttgaggctc taatgaacct
     3361 gtcaataaat caagagtggc tgagatatca ccgatcaatt ctctttcagg ctgcataaaa
     3421 ggatcgattt cagctggcgc ttcatcgatc acaatgatcc gcgcatcttt ttccgcattc
     3481 cagtttcttg cttcatattc gattggatca tagccaatag caataactag atcacttcgt
     3541 ttcaaaagca tatctcctgg ttggtttcta aataaaccca cacgaccaaa gaaatgcgct
     3601 tctaattcac gtgagatcac tccagctgct tggaatgtct ctacaacagg caatgctgtc
     3661 tttccaacta attgcctgat ggctaacgtt tctttttcac ttgatcctcg catacctact
     3721 aatagtaccg gtagttttgc ttctctaatg cgttcagcta ggtaacggat atcatctgca
     3781 gaagcagaac ctaatttagg atcgctcaat ggcttgatga cattcccttg gacaggtgca
     3841 tccactacat cttgcgggat actgataaag ctcgcgcctt tttttgaact cttagccata
     3901 cgataagcat tggcaatcac ttctgaaatt gtttctggat cttggatttc tgcactgtat
     3961 tttgtgattg gctggaacag tgctgcatta tccatgcttt gatgggtcaa cttcaataga
     4021 tcgcttcgtt ttacttgtcc ggcaattgct aaaactgggt caccttctgc tgtcgctgtt
     4081 accaaacctg tagctaagtt actagcacca ggaccacttg ttgcgataac tactcctggc
     4141 tctcctgtaa tacgtccgat tgcttgtgcc ataaatgcag cattttgttc gtgacgagtc
     4201 acgattaatt caggaccttg atcctctaat tcattaaata caccatcgat ttttgcacct
     4261 ggtataccga atacataatc aacatgatga ttaatcaaac tttctactac agatttcgaa
     4321 ccttgtttca ttttttcgtc cattgaacat cgtccttttt atgctttatt tttaattatt
     4381 agttagaaat aatcacaaat aattgataca gtttctaaac ctgtttcatt caaaagtata
     4441 gcacgtttta tttcatttgt ttagccatat gtttcaaaaa atgaaaaaga atgacagttt
     4501 ttacataaaa actgtcattc ttaattgaaa gtttcaattt ataaagtcta cagatccata
     4561 gttctcatca atttacgatt gttgaagtaa atcaaaccaa atacgataga aggacctagt
     4621 agaatacacc attggggctt tacgaaaaag aagagaaaca aacttgtcaa tagtaaaaaa
     4681 tctagtaaac tcacatacca ttttttcaac gtatagtaga acgctatccg tagaacttga
     4741 tttggtttcg acgctggatt tctcacctga aaataccagt agttgatgct aaaggaaact
     4801 gataaacaag caagtaaaac aaacaacgga atcaaccatt ttccataaga aaaacgtata
     4861 aaaaagaaac aatcaaaaat cattatcaca ctaactatcc aagcaaatag ccaataaaaa
     4921 aatcccttta gccaaaattg gcgaaacgtc tttatgaaca aacgtactgg tgaaaaactt
     4981 ttttcttttg ctaattcttc tgtcatacct atcatcacga tgcaacctgg accaataaaa
     5041 agtaaagaaa aaataaagga aaagacattc cttccatcta tcgacaaaag gaacgcagct
     5101 gctatataca agatattgac acaccaaaaa caaaggctac ttgccaatga ttgatacaac
     5161 caataaaaga attccatata aacatttttg tcaaaacgct gctctttcat aatcacctcc
     5221 tattccttca ttcctgatga agcgatccct tctacaaaat atttttgcat cgctaaaaat
     5281 ataaaagcaa caggaagaac agaaacaact gaggcagcca tgattaatgc gtactctgca
     5341 tcaaatagcc caataaacat tttcaaccct aactggattg ttttattttc cgtagaagac
     5401 aaataaatga atggccccat ataatcgttc catgtattaa cgaatgtgat gatcgtcaaa
     5461 ctggcaatcg ccggttttgt aagaggtaaa atgatcttgc gatagattcc ccactctgat
     5521 aagccatcga tccgcgcact ctcatataaa gaatctggga tactactgta gtattgcttc
     5581 aacaagaaca caccaaaagc attgaacgct tgtaatagca ccaacgacca caacgtatat
     5641 gttaaaccca cttttctcat cataatgaac tgcgggatca tataagactg ccaagggaca
     5701 gcaatcgtgc cgatatatgc taaaaataaa atatcgcgcc ctttaaaatt tagtttagaa
     5761 aacccgtaag cagcaaaact agaagtaaac aactgaatca acgtgatcac aacgcttaaa
     5821 aaagctgtat tcttaaaaaa tgtcactaaa ggaatacgag accaaataac cagataattc
     5881 tctaaatgcc aagttttagg gatccattga atcggaatcg tgaacacttc attatttgct
     5941 ttgaatgaag cagaaaccat ccataaaaaa ggaatgatcg tcaataaagc taatacaatc
     6001 agcagaatca tacccaacca ttgaataaag ggcatgtttt ttgaacgaat ttttgctttt
     6061 tttctttcat ttcgaacacc tccaaagtta taatcttcat ttctttgtaa tgattacaat
     6121 ccagaacaac gtagctgatt catttccgtt cttcttatgt ttgtaagcgc ttatttaaca
     6181 agttatgtaa atctctttat ttttttgatt taaaaatgaa aaaatacaaa agcaagactg
     6241 tcatacgtct cgcttttgta ttgaaatttc ttcgattaga gatacttatt actgttcaaa
     6301 agcttctttg aaacggtgaa tcaattcatt gactacaggt gataaaatcc gattcttttt
     6361 ccagactagc atgcagttcg tttcaatcat tgggtcaagc ggaacgaatg tcactcgctt
     6421 tgaaagatga cttacagcac cttctatgac taaagcagca cctacctgat tctcaattag
     6481 cgcaaataca ttgaaaatca aattgtacgt tcctgtgatc agcaactgct ccttttttat
     6541 gcctaaccag tcagcaaaca ttcgctgtat ttctggacgc tttgagatca aaagaggtac
     6601 attcgcaaag tcttcttttg ttatcacgtc tttttttgct aaaacagaat tttctaaaat
     6661 gagcaatccc catttttcct ttcttggtaa caaaatcgtt tcgtatttct ctgtatctac
     6721 tggatctagc aaaatcgcta aatctaacag gcttttttct agtcactccg tgatatcatc
     6781 acttgttccg ctgtggatat gaaattgaac gctgggataa tcacggatca acttttctag
     6841 taatagtgcc aatgtattcg aattatcagc ttccacacag ccgatcgtaa atcggccgct
     6901 gaataattgt ttttttctgt tctcgaattc ttgagtggtt tgattcgtta aatctaaaat
     6961 ttcttctgct cggctttttt agaaaataac ctgcttctgt caagatcatt ttgcggtttt
     7021 cgcgtacaaa cagttctgtt cctagttcct cttctaactc cttcagttgt cggcttaagg
     7081 ttggttgtgt aagatgtaga acttctgcgg cttgagagat tgttccttct tctgcaattg
     7141 tccaaaaata ttttagtaag cgtaactcca tagcgatgct ccttgatttt tttacttgat
     7201 acattccatt ttatttctag ataacacacg atcatatttc ttgatttttc cttgtaaacg
     7261 ttccaaacac tcttgcattt cagcaatttc tttcgtcaaa agttcacttt cttcaatcaa
     7321 aagcttacgc cgctcaaata cagttgcttc tccttcgcta gataggcgaa tatattcaat
     7381 caaagactcg atcgataatc cggcatttcg catacagatc gtaaagtcga cccattttag
     7441 gtcttcttct gtataatcac ggattccgcc ttttgtccga ttaactggtg gaatcaaccc
     7501 gattcgttca taatagcgca atgtatcggc cgaaatgccc ttttcttcac tgacttgttt
     7561 gatattcatc tgatcactct ttctcttaat ttcttgccac tttacttatg tattttattt
     7621 attctacaac tttaagtgta ctccaagtca atactcattt ctattaaaac taagctggat
     7681 tatctatatc ggttgtttgt gtattatttc aaataaaaaa gtcttgtttt tttattcttt
     7741 tttggatata cttttaacaa tttagggaaa ttttaggaaa aggggtataa aaatgacaac
     7801 gtatcagcga aaggtattat tatcgacatc ggcgggaatt gctttagaga atatggatat
     7861 tatgttttta gctttttcat tatcttcaat gattacttct tttcatatta gtggtacgca
     7921 agcgggtttc atttcaacga tcactaattt ggggatgctc attggaggta ttttttttgg
     7981 catcatagct gataaatatg gacgtgtgaa agtattttct caaacggtcc ttctgttttc
     8041 gattgcttct ttattgatgt attttgcttc aaacatctac ttggtttatc tctttcgctt
     8101 tatcgcaggt gtaggagcgg gaggagaata cggggcgtgt atgtccttga tttccgaaag
     8161 tttttcaaaa aaacaaattg gacgagcttc ttcggttgct ggtattggtg cgcaggtcgg
     8221 tgcggctttg gctgcgattt tagctgctgt gatcattcca acgtttggtt ggaagatgtt
     8281 gtatgtcgta ggtgtactgc cggtgttgat ggtcttgttc attcgtcgcg gcttgactga
     8341 accaaaggaa ttcaaatctg tacaatctgc cgaaaagaaa accaaactta gcagactgtt
     8401 tgaatcaaaa catcttgctt ggcaaacatg tggccttagt atcatggtaa ctgtccaaat
     8461 tgccggctat tttgggttaa tgaattggct gccttcaatt atgcaaaatc gattagggct
     8521 ttcggtttct gggtcttcat tgtggatggt cagtactatt ttaggcatgt ctttggggat
     8581 gatgactttt ggcataatca tggataaatt ggggcctaaa ctagcctttt ctttgttttt
     8641 aatttgttca tccttatctg tctatctact tgtgttagcc catagccagt ggagtttggt
     8701 agcagccgct gtgatagtcg gctactttat caatggcatg tatggtggat atggcgcagt
     8761 gattagtagt ttgtatccaa cagaaatccg cgcgacggcg aataatttca ttatgaacct
     8821 tggacgagct gttggtggtt tttcttctat cgtcatcggt ttcttgatgg atcattatag
     8881 tttaactgcc gtaatcttgt ttttgagcgg tatttaccta gtcagtctgc tggtcttgac
     8941 aactcttact ggtgttacag cattaaggga aagcttgcag cattgatagt tgagtctgta
     9001 aatgacaaga aagaggtgtg gcaaaagctt gcatctgcgg taataagacc agccaaatca
     9061 aaaacatgag acactgtttc cgtttggctg gtcgtattac ttgatgcagg gcagcttttt
     9121 caactgctaa tttccttcta tctggcggcg actattcttt gacgtccgtc atttcaaagt
     9181 actttactaa taacttagca taacgaaaaa ctgtggatac actcgtatat tgagcgtctg
     9241 cataatcttc caaatttttc tcaggatgtt gatacatgaa ttcaatcaaa tgataattat
     9301 atgattttct gtagaaatga agataaataa tgtagattgg gaagttctcc tgcttttcca
     9361 tagaaaagcg ccgattattc tcattcttca aaatgacctt atcaggatat ttttggaaca
     9421 attcttctaa atctgttaga taattcaaga tagacttact tgataccttc aattcgtttt
     9481 gtagttcttc tattgaacac caacgttctg tttcaagcag taaatgaatc atttaatcgt
     9541 ttattatata aatgtcaata atctaataat aaaataacgt acatatttta ttattgcata
     9601 cttaataaca gaactatagc aaaaaatccc gtgtatcaac acgggattag attaagtttc
     9661 ctcaatcact attcataaca caattattat aacaagtaag taaacaattg aaaaggacat
     9721 ttattgaaaa cggttgtttt ttacttaagg aggaaattta gcgaaaaaat tagttgcatt
     9781 gtttttggtc gcaggaagtt tgttgagtgg gtgtggtgct actacgtcta ctactcaaag
     9841 cacaagttca gatcaggctt ccagcagcag taccacgatc tccgaaagta cggcagattc
     9901 atcgaaagaa caagaacaaa ttgaaaagaa aaaagaagag aaacaaaaag cggaaaaagc
     9961 tgcactagaa aaaaagtgac agaagcggaa tctgctttgt cacaagctga agcaaatcct
    10021 acaaaagaaa actatgcaac tgcttcagca gctatccaag cgctacctga aaacaaacaa
    10081 gagttgacaa gccgtcttgc tgcaattgat tccacaatca aagcaaaaga agcagaagaa
    10141 gaacggaaaa agcaagaaga aattgcacga caacaagcag aacaagcgca acaagaagca
    10201 atcgcacagc aacaagcaga acaggcgagg caagaagcaa tcgcacagca acaagcagaa
    10261 caggcgcgac agcaatcagc agctgaacag gcctctcagc aggcacaagc cgcagctcct
    10321 gagcaaaata atgaacaaac tgtctatgtc acaccgaccg gttcaaaata ccatacacat
    10381 aaatgtggaa atggtacgta tacccctgcc actttaagcg aagctcaagc tcgtggttta
    10441 accccttgct ccaaatgtta tggaaactaa ttcaaaagtt atactataat aataaaattc
    10501 ttctgaaaca taaaaaaata gaagatggcg ttccagatag taaaccaaaa ataacagttt
    10561 acacgtccat ttcgtcagct tctctctttt ttcatttagt ctgttccctc tgcttttttg
    10621 gacatcttca ttcattcttt ctcaatcaaa tagtacacct ctttttcttt tcgcagttta
    10681 tatcttcctt ttttcaattt cattgaaaac tgtttcgaat gtttatgttt tacatggatt
    10741 ttaagcagga gctcctcatg ttgcaagcga tagttcctgc cttttaaatc agaaaatctc
    10801 actagtccgt attcatcaga agctttcaca aatggaactt tttctctagt aatatagtgt
    10861 ttgccgttga gcgtttttac agaaaagacc attccttcga ttggtcgtcc gtttgtgttc
    10921 tttacataaa aagataaagg atagtttcgc ttagcgatgg ccaaagaatc taaataagcg
    10981 atgagtagcc aaactaagcc gattgatcca agtagtatac cgataaacca aataaaaaat
    11041 ttccattttt tccattgatc aattccttgg atcgcttttt ttgcctcttt tgcttgataa
    11101 ggaatacgat gccccgtcac aagcagtcga tgtgtattga ccatataagg agtacaggta
    11161 agcaaagtca cgagatctct gcctttttgg atacccaaag aatctacttc atcaggtaag
    11221 accacttgga ttttttctac ttgatacgcc aatgtctttc cattgatctg gatgaaaaac
    11281 tggtctcctt ttttcaactt aggcaaatct gtaaataatt ttgcttctgg gagtccacga
    11341 tgcccagaaa gaacggcatg ggtgtttttc ccgccagtcg gataagaact ttcttccaat
    11401 aatgacgttc ctttttgcaa aaatatttcc gttgtctgat cgaatatagg taagctgaca
    11461 ttgatttttg ggatttcgat gactccaagc atatgttcca taaaaaaggc attactaggt
    11521 aaatctttta atgcttttgc atcaactgct tggttgaaac tgcctatccc tggtgatacg
    11581 ttttgctctg ccaactgttg gtttttcttt gtcatttttt cctgctgttt ttttatttcc
    11641 tctgaattct tattcgatgc ttgcttttga taatggatga tcatttgctg ggcaagaaaa
    11701 tcattcagtg aatcttctac aaaaggataa gaaaagatcc cgatcccaac gactaaaatg
    11761 attgccatga agatgtccag tattcttatt ctgcttttat tcatcatgtc cacctatttt
    11821 ctaatatgta tatgaagagg ttgtgaaatc agcgccttga cctgagtatt agcgaagcaa
    11881 acggcaaaat agttcctcct attttgccgt ttgttgagcg aataccaaga gaccgaccac
    11941 ttgtcacaac ctttctcccc atttgaatca ctatcgttta tttatcgtat gatttttgtt
    12001 tccaatagtt taagcttctg tttcttgatg gcgttttttg aaatagatca ctgctcctcc
    12061 aactaaagca gtacctacca agatgaatac gatgatcccc ataccaccag ttgaaggcaa
    12121 gattccttta ggttggttat cgatcgttgt ttcatcagaa gttgttggat catcatctac
    12181 tgtaaattca gtgtacgcag cttcattctt aggtaatgcg tagccttctg gtgcttttgt
    12241 ttcttgaagg cagtatgccc cttgtttcag cccttcgatt gaaaatttcc cttctgaatc
    12301 agaaatcaat ttcacttctt cccagtcaga acctgtgact tcatctgacc aagcatatgg
    12361 gtattctcct gaggcaaggt ttgctatttt tttcattaat ttaacagttg gatcttttgc
    12421 tcttagaata gaaaattctg catcggctaa tttttcttgc gtgttcacat cttgtttgat
    12481 gaagtggaaa tcaccgtagt tttggacatt ttctacagta atcgtgttat ccgtagttgt
    12541 attttcccga ttgactacac cgtttggatt cccaagattg gcttcatctg cgccatggat
    12601 gataccggtt gtttgttgca attttccgtt ttttactata aaagtagttt ctttttgtgg
    12661 attggttaat tgataaccat ctggtgcttt tgtttcaact aaagtataat tgccgtctat
    12721 taatccattg acactgatgg atccttcagt atcagttgtg aatgtcaacg ctgactcagc
    12781 aggatcatag agagcgacac cagtgttttc atcaaaaccg gtcacgaatt gattctctga
    12841 atttttgatc tggaattgcg cccctgctaa cttatctacc cctgaaaagt tcccagcttt
    12901 ctggattttg atattgcttt ctttgatgac atttttagca tataggtgaa tcgtgtctaa
    12961 aggtttgtca gggtctaggg taagcttaac acgatatttg gcgacgtttc gacaccgggc
    13021 tttggtgttt ctacaaagac gtatacagca tctttcccat cagatttacc tggaagagtc
    13081 aggttagcta atccttcacc agaagtcgtt acacttgcga agggtgtagc gtctgctgga
    13141 actgctgtgt ctttacttgg atagtacgta gcagtgatgt catatacgtt gaattcaacg
    13201 ccgttcaacg gttcgctatt cggaaatgtc aattccgttc cgttgtttat ttgattttct
    13261 ggtgctgaag tgaatttcaa tttatgcaaa ttaaccgtaa cattttctgg tgtaggtgat
    13321 acggctgcag aaacctctgc agttcctagg ccgagaacga aaggaagcag catgatagca
    13381 gttcctaaaa acgtccatat ttttgttttg atgtttttca ttttatttcc ctcctattgt
    13441 ttcctttttg ttgtttttta taaaaccaag ctgtccctgt tgaaaggact agcaataacc
    13501 ctaaaattga gaaacctcca agcatctctt cgcttgtttt tggaaagctt cctccatttt
    13561 tttcatttga tggaccattt gtctgcatct gaactggcgt ttttccatta ttcgtcggat
    13621 ttgcttttgg ttcgttgatg atttccaatg gtataggatg aagcggatca taactgcctt
    13681 tttcaacttc aaagagaatg gttgattgac tcaatatata tcctttaggc ggtttaatct
    13741 ccactagttc gtaacttcca aaagacaatc catggacaga aaatgttcca tctttactgg
    13801 aagtaaaaat cgttgctctt gaatcatctt tggctaccca atgatttatc ccgttttctt
    13861 gcgctaagta ttggtcattt tttttgacta gaaattgagc atctgctagt ttttgtttcg
    13921 tttgccgatc cactttgaca aattgtttgc ctcctgtttg gatggcaaga tgtgtttcta
    13981 ctgtttgatt gtcagtcgtc aaacgtacat cgttatcaaa tgtcgttttt tcactgtttt
    14041 ctttcaatgt catttggtaa cggatcgtta atttttttcc agcaaacgaa ctaagatctt
    14101 ttccagaatc tgataagaca aaaccatgtt ttattttttg tgtgtgaaat gtatgaatct
    14161 tgtctccacc aatcaaaaaa tcgatcgttt ctggttctag ccataactga ggatcagctg
    14221 tatctttgat cgtataggtt cctatctcgt tgatgttctc aggaattgtt acggtgatga
    14281 gataagggac tttatcccca tctgcaaaat ctgtctgctt attctctact gtttttgtta
    14341 atgtgagtgc agcaggtatt ttttgttcgc tctttggata caagtggatg gtcgtcaatt
    14401 tatgattaga tgaatctgtc acaggaagca caacgacaaa tggtgctgat ttttctttta
    14461 cttaatctga agtctttatt tccgtaaatc ggtaaacagc gtcacgacct tgctcatctt
    14521 tgtccgatgc actaaaagaa gcaatgccct cttcctcgtc tactgttttt gtcacatttt
    14581 ctgccagtat tttttcacta tcactttttt gtgctagttt tctttgtgcg tcttccacac
    14641 tacttccttc tgaccgcagc ttgtaaaatt catccgagac atcatatata gcgaaagtaa
    14701 cgccgttcaa cccttttact ccttgaaagt tatccgggat atttattccg tcgttttgac
    14761 tgatacttgg catcttatcg ctttcgaaca ttcgattatg caaagtaaat gtaataggtg
    14821 attctctagc tgacacagtc ggagaaccta taaaaaagac cagtaggata aaaaagagaa
    14881 cagcaaatat atccacacgt acattttttg tttttttcat cgctgttcac ctgctttttt
    14941 cttacgatgg ataaaataaa agaagccaga aaaaataaag atcagtccta aaaagacaaa
    15001 gggcagtaga cctatcccac ctgtcgaagg aagcaagaga gtcggattga tcgtcaactt
    15061 agcggtatct gttgcgataa tcgtataatt attgtttaca cttccttcgc ctaaactaat
    15121 cgttactatt tgtgatccaa tatctgtccg gctgttaact gttagggtat aatccaatga
    15181 ttcgcctaag acaacaccgg taacgtggag atttccaacc gaaatcgagt gactttgggc
    15241 atcataatta aaggcagcgt catcgacagt aatcactgcg ttagctttat agatggtata
    15301 agttcctgat gtgttggcta tatcagtaaa tgacaacttg ggatttgctg ctttgacttt
    15361 gtctattccg ctattcgtta gtttaattgt atagctgcca gcattaacca tattttttgc
    15421 tgattgtgca tctgctgtgt aatccatact gctaagtgcc acttgtactg gagtaagcga
    15481 actagtgaca ccctctgcta gcttgacatg gattgacggg acacctgctt ttccatcata
    15541 tgtcttactg ccactgagct ctaccgcacc gctgatttgc ttaatcgtat agacatcaga
    15601 tgtctgcacg atagtaaata aatagtttgt tctggcagct agtttagcta gtccgctttt
    15661 tgtcaattgc caagacgtat aggttccaat ttcagtaggc gcagttactt caactccctt
    15721 ttcattcatc gctttgaaat cagttggatt ccatgtgatt tgatcaatac tgagatcatt
    15781 gtttaaaagg gatgttgatt ctgttttatc attaaaggca tatttaacga tcggtacaac
    15841 agaaagacca tcatagtact tactgccatt tgcattgatt gatactgttg catttgtctt
    15901 ataatatagg atgacttcag aaatctgttt tccatttgcg tcctttacga ttacatttcc
    15961 atttgtatct aaactagcta aaacactcat tgctgattgg ttctttgttt ctatcgctga
    16021 aacagctgta aaggtatatc cgttgatagt aggcgtatta gtcgtcaggt cttgtgcagc
    16081 acttccgtaa gtaagattca ttattttaga aggagcaatc atctgtccaa aatagtcttg
    16141 atacttaata gtgattggtg tttgtttgcc tgtataggtc accgtatcga cgttactttc
    16201 tagggctgtg aaagaaccgt ttgtttgtgc aaaagaatag cccgttactg ctgttggagt
    16261 gataaccact ttgtcaccca catggattcc agataatgta gtcccgtacg ttccgatttt
    16321 tgctgctcca ccattgactg tataagaaaa agttcctgtc cgattcgcat tgacctgaac
    16381 cgttgctgct tgataggttg gaaaatttga aacatttccc ttaccatcaa cgatctgccc
    16441 atttttgatc gagtaccccg tgaatggaat atcatttgct aactgtatac cgattgaaaa
    16501 agtactcata cctcctccgg caggaagccc tccccatgtt gagggtgtaa aagaataact
    16561 tttatccgca ttgggtgtaa ctgaatcgat cgtatttcca gcggtagcca cccccatata
    16621 gatacttttt gggaatgtga tagtagtacc atttactgtg gagccaataa acagattatg
    16681 gatatctgga cggttatcaa taaaagcgat tgctttggtt acgccttgta acgcagtttt
    16741 atccgcactc atcgatccgt taaccgcaac aatgactcca tctttggcat atatcactgt
    16801 atatccatct ggtatggtat gtccgttgaa attcgcagtg gccgcaacag tactaaatgt
    16861 ctggcttcta cctgtttctg aataagcttt ctcccctgct gcttttgctg ctgtgatttc
    16921 tttttctgtc ggatcaatca gttgagtcga ggatgtggtt gaggttgtct cgttgttgct
    16981 agactgattg gtgctttcta tggactgtat ttcagaatag ggtggactat ttgtctcctc
    17041 tgtcgtttgg ttagcaaaat ttgacgtttc tgtataaacg tttacataaa tacttgataa
    17101 gctccctaag aaagtaaaaa tcaagatgat cgaaatcata atgggatgta agtattttct
    17161 tttagataca gctttgagtc tagaaacaaa atgtttttga catatacgtt ggattttatt
    17221 ttgagttttc ttaatcattt tcatccctcc tctcaataaa aaatcatcaa ataatccttt
    17281 acacgttatt ctaaatttaa tttttattat tttattgatg tatagaaagt ttggggcttt
    17341 ttttctcttt ttattctaag gttattttag tcttgtcctt taatacaaag attttatttt
    17401 taaataaaaa atactctttt tttacgatta ttgaaaaaaa aagataaaac ttgattaatt
    17461 gaagtattgc ttaaatttgg aagctatgag ggaagaataa aagagttagg acgggattat
    17521 ctactatttt tctttgtttt aaatatttgt tatctttgaa cataaaagag aaatgtgtcg
    17581 actgcagtgt tgtgtccctc atcacgaata aacggtgttc aattagctga tcttgtattc
    17641 acttaaaaaa tggtaaagta cgagttgcta ttccccattt tcaattttct aacgctcagg
    17701 acaaaatact gttttcacaa cctcattgga ataaaagcat atatattgtt tcaagaacga
    17761 aatcttgtta tctttttaaa gggcatctgt ccaaaatcta attttaggag gtacgttttg
    17821 gtatttattc gtacaaataa aagagaatta tctttattca tgtaacgcat ccaattgatt
    17881 ttttcatgta cgctcctgga acgattttac tgacgattgc ttatttaact gcgaatcgct
    17941 cattagcttt tatcatagct atccatattt tgaataacgt tcttggcttt gtattataga
    18001 tacagatgac aaaaaaagtt caagtatgcg gtgtagaacc aatatcgcat acttgaactt
    18061 ttttcaatga gtataaatta aaacttctaa ttttcaattc ccatcatctg atatgcccga
    18121 ccgtctgaac ttcttttgaa aaagccaaat gtgattagat aacgtctttg ttcgacataa
    18181 tcgccactag aaaatacgtt cagcagtatc gaattaacct ctgtttctgt ataagtcact
    18241 ccatggtcaa agcgtttcaa gacttctttc aataggatgt atttcttttt tgttgaactt
    18301 ggtacttgtt ttacttttcc atcattcatg aatgtgtcta aaactttcat cttttcttgc
    18361 tccgtataag tataaaccat cttctttctc cttttgattc taagaatact ccatttatgt
    18421 tagaatattc tggatttttt attaaagcgg ttactcttaa gaaagatatt atcatagtgc
    18481 tctttttttt actactactt ataggtagat ttattctaat ccattattct cccctaccaa
    18541 taattaaaga aaaaactgga tcagggtgtt caaattacat gctaagaaaa aagaaccctt
    18601 gaataccaag ggttctttct cagtctgata acaatgatta acgacggatt tctttgatac
    18661 ttgtgaaaag tctctacagt attgatataa aaggattttt attaactctg actacacttt
    18721 gactaatctg ttaaaaaatc gttttttaag aatcctgtaa taccagtact ttcaccgtct
    18781 ttttaatctt ctaaagaact aaaagcagcc agaggcttta acttttcagt tgatgttaaa
    18841 tggcgatata tttttgaggt tgtactatct gtatgaccta aacgatcctt caaaacatcc
    18901 caactgcaat actctgccac taaactagcc atagtatgac gagccaaatg ggtatgaaac
    18961 tctttgttaa ttccacatcg ctctacacaa cgcttaacat gatcgtttac atattcacga
    19021 cgataaggaa ctccacactt accaggatca aggtaaacaa ataaataagt ttcatctaca
    19081 acaaaattgc tatttctttt tgttttattt aatacctcaa ttaattcatg caatttctcc
    19141 attactaatg gtgtgacagg tactcttcta gcagaactaa cagtttttgt agtttcaatt
    19201 cgaaaatctt tgctttttcc ttttactgct gtttgtttat aaaacaagat ttcatttcgt
    19261 ccaaaatcta tatcttgaag ctgtaaagcg agcgcttcac tcacacgaca tccagtataa
    19321 aacaatacat gaaaaagcac agacatgggt aactcctcat aaattatgcc atagtttaaa
    19381 aaagttcgta attcatccac tgttaaaaac ttatcttcta aactgttgac agactctttt
    19441 aaatctttaa ccgttaactg atatttaggc attttaacga acttcattgg attctcattt
    19501 atcaaattct ttttcttaca ataattaaaa accatattta aaacctggac aagagattgt
    19561 aaactatttt tggcataacc aacatcaacg ccatcaattc ctttttcttt taaaataaaa
    19621 aggaattttt gtagttcttc tgaagataga ctgcttaatt tttcatcacc aatccatcga
    19681 ttaagtattt tcacctgttc ttctcgctta tcatatgttg ccaccttttg ttcgtttagt
    19741 gatcgataat attcaaacca cttttgacta actacactga atttgtcatt actagcaatc
    19801 cttttttgat tatttctgtg ttgtttttcc atttcaagat aagcaagctg agcttcacgt
    19861 ttagaattaa aacctcgtct cgtagttcga aaagcctgaa aataccaagt aatttttccc
    19921 gttgcagtaa tgtgtttttt aaaacgactc atattttcca tttgtttcta cctccaatta
    19981 tgaaggaacc caacaaccgg aaactcttga aaatctttct atttatgaaa taattataca
    20041 atatacagac gaaaaaataa attctacaat attcgtaata aacgaaactg atcacaaaag
    20101 tattcttcct ttcctttttt tacccactcc ataatatctt ctctgaagta atgcccccta
    20161 ataccaaacc agcatcttgg aaaattatct agttttataa gacgatttaa ggtactatca
    20221 ctgatttgaa attgttcttt tagatcttta tttgttaaaa ctaacggtaa ttcatttatt
    20281 ctctttattt tattgataga atcctctata ctttctagat aaaataaata gttttctaat
    20341 tctgatgtat tgcttttatt attgttcttt aacatgagtg tctcttccct tctattatca
    20401 atcgattgat ctatcagcta cttttgtcaa aagtacatag gaattttacc tctccccaag
    20461 tttacggcga gccttacgga agtatcatta tcccaacttc aaatcatcgc tttttattga
    20521 ccataaaaat tattttagaa tatttatcgc tccttcttta taataagaga tcatggcgca
    20581 actaaaatat gctccttgaa gtgttaacgt actgatagaa tatatttagt tttcaatgaa
    20641 caatagtttt ttgtacataa aagaatcttc taatagttaa gagattagta gattctttta
    20701 aaaaaagtgg aaagggaact ttttagttag ttagttaatt ttaaatttca atatagcttc
    20761 tattaattta ttttctagct gtcttaacat atattcatct acataggtaa taaattgacc
    20821 cacttcgtta aatccatttg ttaaacataa cttagctata taactatgat aatgtttgag
    20881 tatcatcttg atcgaaattg gatcttcttc tagagcagat agaataatgg ataatggaac
    20941 caatagatac tgccgctttt tttgtttatt taccatagat catacctcat tttcaattgt
    21001 tcaactgcct tcgttcgttg ataccagata ccactcgtac tcatattgag aattttaccg
    21061 atttcagcat cattaaaacc tgcaaaataa taaagtagga taatctttct tcttatatcc
    21121 gataactgat caattgctgc tgataacttc tgatctgaaa ttagtatctc cattccgaat
    21181 aagaaatagg catcttgttc atcagaatga gtttgggagt actcaccttc atttaaatat
    21241 gtcaatggtt cctcatttac ttttgttcta atgtttcgtt ttcgaataga cttagcttcg
    21301 tttcgtaata cttttttaca ataggcatca aattggtgtt gtatagattt ctcagttttc
    21361 ttttgcatga taaatatgct cctttatccc ctttccacta atacaacgat cagagagtgc
    21421 ctttttttcg aaaaaaaata caaaataaaa atgagctact tattttactt ctccatatcc
    21481 aataacacct tgtatttcac aaaaataaat agacttttgc agttatgctt agcttattag
    21541 taattacaat tattttttga aaaactatgt aatactatca atctcatata tgctgaactt
    21601 ctatcgtcgg taaacaaaaa aacctatcga tatactaata ctggctacaa atctcatatt
    21661 caaattaaag agttttttat aattaaagtt atatcgtcta tgagtagaaa acacgtattt
    21721 ttgtttcata cgctatgcaa aaaaactgat taaaataatc aataaaataa ataggaaatg
    21781 atataatggc tagaaagaaa agaattcctg aaaccgaaat aattagctga atcaattctg
    21841 aatacctata aacctgaatt ctgtcgatga catgaaagat actttaaaat acgtctttaa
    21901 ttttttcttt aaaaagatgc ttcaaagaaa actgaatagc cattttctgc ccattctaaa
    21961 gagcctaagg aacacaataa ccatcaaaat gactgtgaaa ataaacctct taaaactaaa
    22021 gttttggtga aatagcattt aatgttccta aaaaacgtaa gattctttaa aacgaaacta
    22081 atttctaagc agaaacgaga tgtagtcaac atcaaagaga agattcgttc catgtataaa
    22141 cgagtagtaa ctcaaaaaga tatcatctca accgttgaaa ttatttatag tcatagtatt
    22201 tccaatagaa tgattttaga taccactgac actgccctta tcgaattaga agattggcaa
    22261 tcctgaccat tgaccaagcg tatgctcttc tatttgacga ttgtatattg cttttcaaga
    22321 aaactagaca aactataact aatgtaaatg ttaaataatt gcatttaata gcaatatttc
    22381 gggtaagcca aataaacgtc ttatttttcg tttttttgac aaaacgttca atggatatac
    22441 ataggggaat ggagattcaa ttcaatttaa ttttatttct tcaaaggata tttgatgctt
    22501 tagaagatga aaaatgatcc ggacaagttt ttttgcaaca tgagtgataa ctacactata
    22561 gtgttttact tcaacctttt ttatacagat atgctttgaa tattggcaag aatcaagcag
    22621 ctaatttggt agcttgtatt aaagcccctc gcaaggaagt atagccacgt taatacagaa
    22681 ggatctaaac cagcaaatgc agttgtccaa gaaatttaaa attttatctg agataaaatg
    22741 acactaccta atcgattttt tacgcctgtt agtaattagt gaatttattt tcgttataac
    22801 tgatcgattt ctatgtcgat tatagctatt gggagttcaa tggtttcaat tatttggaac
    22861 aactctaatt cgagactaga agtcgaaaag ccaattgttt attttgaagc tacttttata
    22921 ctatcaattg tttcagaagt aagttttcat ccacaatgct ttgttagaac ttaaaataag
    22981 tttttgtttc gacttctact aatcttttta ggcgggggat aatctttaaa catattgtaa
    23041 atgtatttac tatgattatt tgctttacct acaattagcg ctatctcagg aaacataata
    23101 tctagcaatc gaacaaattg tgttctcagt tgagctcgtt tctctatagt acaagaacga
    23161 tatctaataa aaaactttag ctcttgatta aaaagtgcaa ttactattta acttccacag
    23221 tttagttgac aaaccctcga gatatgaaaa aacgctacgt gcttcgtctc taaatgctac
    23281 acatataacc ttcgatagat ttcaaaatca atgggcactt actctttgat gtttcaattg
    23341 ctaaaatgct ggattttcat taaaagtaac acatccaccc gtaaaaacgg gtggcatttt
    23401 acaactttag aagaaggcag tactcacata ctccataaat agagctttct aaaaaatact
    23461 ccaccattct tcgctcgcta ctttatttcc ttttcttcat tttaaatgga ttaacgtatt
    23521 ctcgtttact tatcctatcc aggattcaat catctgcttc ttgctttcat atatattttt
    23581 caatggtttt ctgattcaga ctgattgtac tcacgtaata gccctttgac cagaaacttt
    23641 tatttccata tttatagact aagtttccat actgctcatg aataaacatc gcacttctta
    23701 ctttcaagta gcccatgaac tgcaagatac ttattggggg cggtactttt accaacatgt
    23761 gaatatgatt tagcattgcg gctgcttcga ttgtttctac ctttttcccc tcacaaaact
    23821 tccacaaaat tcttccaata tcttttctta attccccata gattactttt cttctatatt
    23881 tgggtgtgaa gactatgtga tccttacaat tccatctagt atgtaataga tttttgtcgt
    23941 ctttagacac agaaaaactc ctttcgataa aataagcggt tgatagacac ttattagtat
    24001 atcgaaaaga gtttttatag ctaaagctat tccatccacg cgtagaaacg ctcgatttcc
    24061 ttgtttcata cgccctacga acaaaactag ctaaaatcat taattatcac aatgaatccg
    24121 gctctataat ttatgggact gaagaatcca ttctgattca tcagtcccat tttgtacaac
    24181 tcatcagtcc tcgttaagga agtgaacttc attaacaaaa taaatatcaa ctatatttga
    24241 cttagagcca gtatttatgt gcgaaagaac tacactcaag taatcaaagg gaacaaaatc
    24301 aaaactaggc gattgtcacg gtacttaatc atataataat caacaagtct tctcctttag
    24361 acttgaaaaa tatgcgattt accatctgta acaaatataa gtttattcaa ctatgtatct
    24421 gttaaaaaat catttaattt ccaaatagac aaaaaaattg cagcgttaaa aactcttaac
    24481 tacttagctc ccacagttta gttgacaaac cccatatatg ccactcatat atatatttct
    24541 ttcgttttta attattttag gtatgtctta ttctttctta tacacgattg ctttataatc
    24601 tattgatttc tatttgtttt atttagtata tatttcctca tttattctca gcatctaatt
    24661 taagtgcaca actatatctt tagggttccc acaaaacatt attctattaa ttgttcatcc
    24721 ggctcccaag cttcactaaa atcatgatct ttaaataaag aagccaatcc cgttatttgt
    24781 tttaaacgta aaatttcatc aggatccatt cctaaatgtt tggaaatcca attatcagaa
    24841 cgtcctaatt catgcaaatc ttttattata ttactcatta aatctacatc atgcgtacct
    24901 cgtgcacgat tatgtcttat tgtgcttgcc attctttgat caataggttt atcaatgatt
    24961 gaaactggca agcatcctcg ttctctatca aaaatatctt catactctaa catcacccta
    25021 tatctatgaa atccatcgac aatgatatat ttatcttctg aatcatcata cacacataca
    25081 ataggcattg tgtaaccatc agctttaata ctgtcgtaca ataatttcat ttctggtgga
    25141 gcaactgaat taggattata gtcatttgga aatacttttt caatagggac tgatttaata
    25201 tcatacactg gacttttttt attattcata aaagcacacc ttcatcttat cttaatcttg
    25261 ttccatacaa taaaagctat taaaatttat cttcatacaa caacaccatc acaaatcatt
    25321 aatatcttta tatttatttt ttatataatc taaacgtttc tgttgttgtc tagttagacc
    25381 aaatcccata aatcggcaaa tatgatcatt ttttaaaatg caaaagcaca ttcttttcca
    25441 gctaggaaca tctttagttg tttttatatc gtcagtatca tcaggtattt tctgcagaaa
    25501 aataattctc gaatttttaa atattgtata attagaaatt ccatttcttt taattttata
    25561 tccgtgttct tttagttctt ttattgtttc ttcttccaat cctccaccaa tattatgcca
    25621 aaaattaatt gatttattaa atttaattat ataactatct cttaactcct taggtagagt
    25681 acaaagtaaa aactgagtat aagacttcca tgtatgtcca ttaggtagcg aaacagttct
    25741 gtatcccatc gctttagttt ttccataaat actggcaaaa ttagctcctc tcactcttcc
    25801 caccaattta acccatattc caggatctat tactctgtat aaatttaatg aatcctttgc
    25861 ataatcttga aatggtgaag ccacacgcat ctgggatata tttaagcctg ctttataata
    25921 taaattatac aattcattat atttgaatcc aaatttaaaa taagcatgcc atacatcttc
    25981 cgtagtccaa tcatagagcg gtgaagctgc ccaaagatct ttaaattgac gtgtaatcca
    26041 acattgattt ttatatccat actttttatt aataaatcca ctatatcttt gtagagattc
    26101 atccgcacga atccctaata aacagattgt ttttttatct ccgtgtgact gcttatacca
    26161 gcgaccaaac tgtttggcta aatcttcttg atgcatttta taacgataag ttgtcatgat
    26221 attattatcc atgttaatca catactcatg ttgtggcatt ggacgtaccc aagattcttt
    26281 tttcttatca tcccacggaa accaatacat ttcatagcta cttaaagccg ttcgagtagc
    26341 cataggtaaa catacccaat aacgttcaat taaattcgat gataatgatg taaaagtttc
    26401 ttcgacatac tttgttgtag cactatactg agcctcaaaa tcttggtgaa aaaccccaac
    26461 tactctagaa ggataataag tatctctaaa atctagaatt aattggagca acagtccact
    26521 atcctttcct ccagaaaaag atacata
//
LOCUS       DAZABD010000041        26150 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000041 DAZABD010000000
VERSION     DAZABD010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26150
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.041"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            227..1231
                     /locus_tag="ACHALG_001751"
     CDS             227..1231
                     /locus_tag="ACHALG_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN2587137.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            1232..2176
                     /locus_tag="ACHALG_001752"
     CDS             1232..2176
                     /locus_tag="ACHALG_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN2587138.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            2176..3138
                     /gene="opp4B"
                     /locus_tag="ACHALG_001753"
     CDS             2176..3138
                     /gene="opp4B"
                     /locus_tag="ACHALG_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFN2587139.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            3165..4079
                     /locus_tag="ACHALG_001754"
     CDS             3165..4079
                     /locus_tag="ACHALG_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN2587140.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            4105..5886
                     /locus_tag="ACHALG_001755"
     CDS             4105..5886
                     /locus_tag="ACHALG_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFN2587141.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNDAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            6234..6920
                     /gene="rnc"
                     /locus_tag="ACHALG_001756"
     CDS             6234..6920
                     /gene="rnc"
                     /locus_tag="ACHALG_001756"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFN2587142.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            6940..10521
                     /gene="smc"
                     /locus_tag="ACHALG_001757"
     CDS             6940..10521
                     /gene="smc"
                     /locus_tag="ACHALG_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFN2587143.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLLKEAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEVLAKEQPKMYQLMNQVQQLRARQKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCSVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            10530..11339
                     /locus_tag="ACHALG_001758"
     CDS             10530..11339
                     /locus_tag="ACHALG_001758"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFN2587144.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            11351..12349
                     /gene="ftsY"
                     /locus_tag="ACHALG_001759"
     CDS             11351..12349
                     /gene="ftsY"
                     /locus_tag="ACHALG_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFN2587145.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            12536..14803
                     /gene="gshAB"
                     /locus_tag="ACHALG_001760"
     CDS             12536..14803
                     /gene="gshAB"
                     /locus_tag="ACHALG_001760"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HFN2587146.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTGRNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFADQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDGIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(14892..15344)
                     /locus_tag="ACHALG_001761"
     CDS             complement(14892..15344)
                     /locus_tag="ACHALG_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HFN2587147.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            15494..16444
                     /locus_tag="ACHALG_001762"
     CDS             15494..16444
                     /locus_tag="ACHALG_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFN2587148.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLAT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFPQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            16437..17396
                     /locus_tag="ACHALG_001763"
     CDS             16437..17396
                     /locus_tag="ACHALG_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFN2587149.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFAWALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     GFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            17393..18148
                     /locus_tag="ACHALG_001764"
     CDS             17393..18148
                     /locus_tag="ACHALG_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN2587150.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAIQIMQILKKLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            18171..19124
                     /locus_tag="ACHALG_001765"
     CDS             18171..19124
                     /locus_tag="ACHALG_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFN2587151.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQAETALTIK
                     DSNGDQIEVPKNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPNLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(19647..19943)
                     /locus_tag="ACHALG_001766"
     CDS             complement(19647..19943)
                     /locus_tag="ACHALG_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HFN2587152.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            20298..20654
                     /locus_tag="ACHALG_001767"
     CDS             20298..20654
                     /locus_tag="ACHALG_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFN2587153.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            20664..21563
                     /locus_tag="ACHALG_001768"
     CDS             20664..21563
                     /locus_tag="ACHALG_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFN2587154.1"
                     /translation="MGNLAIISDLHVDINKFGEPELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFANKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            22083..22811
                     /locus_tag="ACHALG_001769"
     CDS             22083..22811
                     /locus_tag="ACHALG_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFN2587155.1"
                     /translation="MKKEIVLTAATMLFSMVASTTFVSATEVYPKEYNTEGTITFEAG
                     DEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYAA
                     ADVMNDGSRKPTYVQVTDRRGTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAVS
                     LVDPTYMPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSVP
                     GKSVKLAQQYSTKLVWTLEDTPNN"
     gene            22956..24983
                     /locus_tag="ACHALG_001770"
     CDS             22956..24983
                     /locus_tag="ACHALG_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HFN2587156.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISVVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENENLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDVEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            25091..26150
                     /locus_tag="ACHALG_001771"
                     /pseudo
     CDS             25091..26150
                     /locus_tag="ACHALG_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
BASE COUNT         8709 a         4559 c         5557 g         7325 t
ORIGIN      
        1 tttttatcct tcttttttta atctttttga cacgtaaaag tcatttttct gataattctg
       61 gcgataaaca gaaattatgt tgcaaagtaa agcggttttc tatataatta ttaataattc
      121 attacacaaa gataagaata acctttgtta taaaacgatt tattctgtgt aatgtccttt
      181 tttattaaga aaaacttcca tttaggaagc agagaggggt attcgattgt ccgataacca
      241 attattagaa gttcagaacc tacatacagg attccgactc aaagatgact attacgatgc
      301 agtagatgac gtttcattta ctttagacaa aaacgagatt ttagcaatcg ttggggaatc
      361 tgggtgcggg aaaagtacct tggctactac gattatggga ttacttgatc ctaataacac
      421 aaaaattaca ggtgagattt tatataacga tctgaattta atcgacctga acgaaacgct
      481 ctataataaa atccgcggaa atgatatcgg aatgattttc caagatccgt tgtctgcatt
      541 aaatcctctc atgagaatcg aagaccagat caaagaagga ctttcctatc acacaaagat
      601 gaatgcagaa cagcgtcaag ctcgtgcact agaattgcta gaacaagtag ggattcctaa
      661 tcctgcgaga gttggacgcc aatatcccca tgaactatca ggcggtatga gacaacgtgt
      721 catcatcgca atcgcaatcg cctgcaaacc tccaatcgtc attgcagatg aaccgacgac
      781 agccctagat gtaaccatcc aagcacagat tcttgattta ctgaaagacc ttcaggaaga
      841 aacaaaaaca gggatcatct tgatcactca tgacttagga gtcgtagcag aaatggctga
      901 tcgagtggca gtgatgtatg gcggacagtt cgttgaagta gcaggtgtaa aagaactgtt
      961 tgatcatcct aagcatcctt atacacgctc tttactgaac tctataccac aagaaggaaa
     1021 tcatgatgca gaactccatg tgatcgaagg tgtcgttcct tctctgaaaa acatgcctcg
     1081 taaaggttgt cggtttgcac cacgtattcc atggatacca gcaagcgatc atgaagaaga
     1141 gcctatcctt catgaagtcg caccgaacca cttggtacgt tgctcctgct ataagcattt
     1201 ccactttaga gacgaaaaag gagaggtgta gatgacagaa ttaatcacaa tcaaagacct
     1261 gaaagttcat taccctattc gcagtggatt cttcaatcga gtaacggatc atgtatatgc
     1321 tgtagatggt gtggatttta ttatcgaaaa aggaaagaca tatggtttag tgggagagtc
     1381 tggctctgga aaatctacta cgggaaaagc cgttgtggga ttagaaaagg taacagccgg
     1441 acaaatcatg tatgaaggaa aagatgtaac gaagcgaagc aaccgcaaaa aaatgggtta
     1501 taacaaggat gtgcagatga tcttccaaga ttcaatgtcc agtttgaatc ctaaaaagcg
     1561 agtgttggat atcattgctg aacccatccg aaattttgaa cggttaagcg atcaagaaga
     1621 aaagaaaaag gtaaaaggac tgctagatat cgttgggatg ccggaagatg cgctatataa
     1681 atatcctcat gaattttcag gcggacaacg tcaacgtcta ggagtagccc gtgctgttgc
     1741 taccaatcca aaactgatcg tagccgatga acctgtatct gctctggatc tatctgttca
     1801 agcgcaagta ttgaatttca tgaaacgaat ccagcaagaa tacggcctca gctatttatt
     1861 catttcacat gatttaggtg tagtaaaaca catgtgcgac aatatcgcaa ttatgtacaa
     1921 aggtcgattt gtagaaatcg gtacaagaga agacatttat aatgacccgc gacatatcta
     1981 tacgaaacgt ctgctttcag ctattccgca aatcgatgta gaaaataggg aaagccacaa
     2041 agaaaatcga cgaagagtag aacaagagta tatccaaaac caaaaagaat actatgatgc
     2101 gactggtcga gtctacgatt tgcgtacgat cacaactaca cacaaagtgg cgttgaaaga
     2161 tggaggtgca agctaatgtg gaaaacgatt ctgcgacgtg ttttattgat gatcccgcaa
     2221 gttattattt taagtgttct gatcttctta ttagcccaag cgatgccggg ggatccattt
     2281 accggtttga tcaatccaaa tcaggatccg gcagtgatcg aacagatgcg acaagcagct
     2341 ggcttgaatg acccatggta tgtccagtac ttccgttgga tcggaaatgc tttgcaaggc
     2401 gattttggtc aaagtttctt atacaaacgc tctgttgctg acttgatcgg cgaacggatc
     2461 atcaacactt tatatctatc gatcttgaca gttgtcctta cttatttgat tgctgtgcct
     2521 ctggggatgc ttgctggccg gtatcaaaat tcaatcttgg ataagattgt cgtaatctac
     2581 aacttcttta gttttgctgt tcctttattt atcttcggct tgatcatgct atttgttttt
     2641 ggttatcgtt taggctggtt cccaacaagt ggatcacaaa cactaggttt gcaaggtgga
     2701 tttatcactc aatggataga ccgtttgaaa tatattctgt taccatcaat tacccaagca
     2761 tttttggcga ctgccgtcac aatccaatat ctgcgaagtg aagtaatcga ttcgaaatcg
     2821 ctcgattttg tcagaacagc ccgttccaaa ggggtaccga cgaataaagt attcagtcga
     2881 catattttcc gaaatgcagc gttgccgatg gcttctcaaa tggggtatga aatcacttcg
     2941 ttgattgctg gttctgtagt tatcgaaaag atttttggtt atccgggagt cggaaaacta
     3001 tttattgatt ccatcggcca acgggactat accgtgatca catccttagt attgatttta
     3061 ggaatcgcga cgctagtggg gacattactt tcagatatca tcatgagtat cgttgacccg
     3121 cgtattcgga ttcaataaaa ccaatcagaa aggagaatgg atcgatgagt gaaaaaaaca
     3181 atacagcagt tcaagaaagt attcctccta tggggatacg aatgattgct cgagaattta
     3241 aaaaagataa aattgctatt ttttcattag gtttgctagt ggttttatta ttagtgatct
     3301 ttatcggtgc gatgctgacc gatcaagaca aagtgatgta tgttagtatc ttcgacaaat
     3361 acgctgcacc aggaacagtt tcgacacaag gacaaaagtt cattctggga gcagatgaag
     3421 gcggacgtga tgtattagga caattgatca taggtgctag aaactctgtt acgatcggat
     3481 ttgcgatcac gatccttact tcgatcatcg gggttggatt agggatcatc tctggctact
     3541 acggagggat cattgacaac atcttgatgc ggatcgttga ttttatcatg attctaccga
     3601 tcatgctgat cattatcgtc ttcgtatcga ttatttccag atacaatgtc tggtccttta
     3661 tattcatcat gagtgcattt tattgggtag ctaaagcccg attattccga agcaagacat
     3721 tatctgaagc aagaagagat tatgtcagtg cctcaaaaac aatgggaacc aatgatttta
     3781 aaatcatgtt ccgcgaaatc atgccgaatc taagttcatt aatcattact aatttgacga
     3841 tgaactttgc ggcaaacatc ggtatcgaga caactttgac tttcttaggt ttcggcttgc
     3901 cggcaaatgt acctagttta ggtacattga tcggttatgc aagtaacgga gaagtcttag
     3961 tgaatcggca atggatctgg ttacccgcat caatccttat tttagttttg atgttgagta
     4021 tcaattatgt tggacaagca ttcaagcgtt ctgcagatgc gcgccaacgg ttaggctagt
     4081 atagaagcga agggggaaat aggaatgaag aaaacgattt ttggatttgc agcgctgatg
     4141 tctgctgtcg ttcttgcagc atgcgggggc ggaggtacag atacctctgg aaacaactct
     4201 ggaggcaacg gcaataacaa agaggaatta acttttccaa ttgcaacaaa aaataatgat
     4261 gatgcaatcg aagacggcga attagaagct gcggtcgcaa cggacagtca gtttaaagga
     4321 ttgttccagt gggagtttta ccaagattca tatgatgcag cttttatggc tccttctcat
     4381 gaaccgctat ttgcaaacga tgacgatttc agaatcacag acgacggagc tgcaacgctt
     4441 gatctagatg aagatacgaa aaaagcgaca atcaaagtaa aagacaacgt caaatggtca
     4501 gatggtgaag atgtaacggc ggacgatgtg attttccctt acgaaatcat tgggaataaa
     4561 gactacactg gtatccgtta tgacgacact ttccgtaaca tcgtaggtat ggaagaatat
     4621 catgatggaa gtgccgacac aatctccgga atcaaaaaaa tagacgacaa aacagttgaa
     4681 atcgaatata aagaagtaaa cccaagcatg cttcaatcgg gtggtggtat ctggtcgtat
     4741 gcagcaccaa agcatacgtt agaaggtgta gcaatcaaag atatggaatc aagcgatcaa
     4801 gtacgtaaaa atccagtgac ttttggccct tattacatga gtaatatcgt tgcaggagaa
     4861 tcagtagaat acttgccaaa cgagcactac tggaatggta cgcctaaatt gaaaaagatc
     4921 acgatgaaat ctgtaccgat ggcaagtgca actgaagcat tgaactctaa attatatgat
     4981 atgattttcc aaatgccaac agatacttac gatacctata aagaaattgc tggctataca
     5041 aacttaggcc gtgaacaaac ttcttatact tatctaggat tcaagttagg taaatggaat
     5101 gcagatacaa agagtgtgga atacaatcca gattcaaaaa tggctgataa atcattacgt
     5161 caagcaatgg gctatgcact agataatgat gcagtaggtg aacgtttcta tgcaggtttg
     5221 agaagcaatg cgacttcatt gattcctcca gtattccagt cataccatga tgatgagatg
     5281 aaaggattca cacaagatct agacaaagcg aaaaaactat tagatgatgc tggctacaaa
     5341 gatgtggatg acgatggttt aagagaagac aaagatggaa ataaactaac aatcaacttt
     5401 gcttcgatgt ccggtgggga aacagcgcaa ccattggcag attactatat ccaacaatgg
     5461 aaagaaattg gtttggatgt ccaatacaca acaggtcgtc tgatcgaatt caattctttc
     5521 tatgataaat tagaaaacga tgatcctgaa atcgatgttt accaagctgc ttggaataca
     5581 ggaactgatc caaatccaag tggactatgg ggaccaaatg ctgcgttcaa ctacactcgt
     5641 tatgaatcag aagaaaatac aaaattgatc aacgatatca gttcaagcaa agcatttgat
     5701 gaagagtacc aaaaagaagc atatcaaaaa tggcaacaat atgcgtttga agaagcattt
     5761 gcgattccaa cgttgttccg taatgaagta ttgccagtca acgaccgtgt caaagactgg
     5821 gattggtctt atgatgtacc aaacccatgg gcaaatgttt ctgttacagc agattcacgt
     5881 tcataatcac taaaaatgac cgaaaaaggg cgatgtcctt tttcggtttt ttgatatttt
     5941 tctgtttatt tctctaatat tcaggtcaag gcgctgtttc catgacttct ccaataaacg
     6001 gtgttcaaag tccagtttct cggtattaag tcaaatttat gcaaaaacat ggaaagtcat
     6061 ttttacaaaa ttttttcttc atactcgaaa ctaaacgaat ttttcacaac ttctcgtcaa
     6121 tcactaggat tattaaagct ttacttacct tttgaccgaa ccatttcaaa tttgacaaaa
     6181 aaccagtata ataaggaagt atcgaaaaaa tatccataaa aggaatgtgg aaaatggaca
     6241 accagctgac aaaagaacta aaagaaaact ataacatcgt ttttcacgat ctaaatttac
     6301 ttgaacaagc ttttacccat tcatcctatg tgaatgagca tcgcaatctg caattatcgg
     6361 ataatgagcg acttgaattt ttaggagatg ccgttttaga actgatggtt tctcagtatt
     6421 tattccgttt atatcctcat ttgccagaag gaaaattgac aaaaacacga gctgcgatcg
     6481 ttcgcgaaga cagcttgtcg aaatttgcca aagaatgtca ctttgatcaa tatgttctgt
     6541 tgggcaaggg agaagagaat tcaggaggac gtacgcgacc agcattgctt tgcgacctat
     6601 tcgaagcttt tttaggcgcg ttatatcttg accagggatt cgacacgacc catgcattca
     6661 tcgaaaaagt gatttttcct aaagtcaaag caggtgcttt ttcacatgag atggatcata
     6721 aaacaaaact acaagaagtc ttgcaaaaat caggtgatgt atcaatcgaa taccgattga
     6781 tcaatgaaga aggtccagct catgaacgag tattttggat cgaagtttat gtagatgatc
     6841 aattgattgg cactggacaa ggaaaatcga aaaaattagc agaacaagct gcggcggaaa
     6901 atgcattggc agcactgtaa aagtaagtga gggaaagctt tgtatttgaa acgaattgaa
     6961 atagccggtt ttaaatcatt tgcagacaag acagtcattg attttgaaaa tagcgtcaca
     7021 gcagtggttg gaccaaatgg cagtggaaaa agcaatatta cagaagctat ccgctgggta
     7081 ttaggagaac aatctgctaa aagtctgcgg ggcggaaaaa tgccggatat catctttgcc
     7141 ggttccgata cccgtaagca gctgaatatt gcagaagtca cagtgatttt agacaatagt
     7201 gatcattatt tgccattgga gtacaatgag atcagtgtaa cccgccgtta tcgacggact
     7261 ggggaaagcg agtttttcat caataaacaa agctgcagat taaaagatat ccaagaattg
     7321 ttcttagatt ctggattagg aaaagaatcg ttctcaatca tttctcaagg aaaagtagaa
     7381 gcgattttca gtagtaaacc ggaagaccgt cgtgggatct ttgaagaagc agctggtgtc
     7441 ttgaaataca agcagcggaa aaagaaagca gaacagaagc tttttgaaac agaagacaac
     7501 ctcagccgcg tccaagacat catccacgaa ttggaagaac agttaactcc tttggccgcc
     7561 cagagtgagg cagcaaagga gtttttacgg ctaaaagaaa cactgactca aaccgatgtc
     7621 tctttgatgg ttgccgaaat caagacagca aaaaaagatt gggacaacaa acaagcacag
     7681 cttgcgaaat tcaatctaga attaggcaaa ctatctgagt cgatccaaga acaagaatcc
     7741 attttggcga agcagcgtaa agaaaatgcg caagccgacc gtctgatcga aaaaaatcag
     7801 caagtattat tagatctttc ggaaaaactg aagcagacag aaggacaaaa agatgttttg
     7861 caggagcgga caaagcatac acaaaaaagc tcgcaagaat atcaaacttc attagcagaa
     7921 gcacaaaaaa aggttaaaca ttttgaaaag cttcaagaaa gcttgttgaa agaagcagca
     7981 gaaaaagaaa cagagatcca aaaagctgaa gcaaatctca taaaaacaca gcaagaatta
     8041 gaaaaatacc aaaaatcaac gaaagaatta ttggcagaat taagagatca atatgttgat
     8101 ttgatgcaag aacaagcagc tgtaggaaac gaactgaagt atctagaacg tcaatatata
     8161 caagaaacag caaaaagcaa gcagacgtta gccaagcaat cggaagtaga agcatcagta
     8221 gaccgattga tcttacaaaa agaagaattg acacaaaagc aggcgcagct aaaatcatct
     8281 ttaactgaaa caaaagagaa gttagaaatg atccaacaaa acgggaaaaa gttccaagaa
     8341 gtattagcaa aagaacagcc taaaatgtat caattgatga atcaagtcca gcagctgcgt
     8401 gctcgtcaga aaagccttca agaaatccaa gaaaactatt ttggttttta ccaaggggta
     8461 cgattggtct tgcagcataa acagcagctt tcgggtattg tcggagcagt ggctgaactg
     8521 atcgatgttc ctgcagattt cacgttagct atcgaaacag ctttaggtgg ggcagcccag
     8581 catgtcatcg ttgaaaatga aaaagatgct cggcaagcca tcacgtattt gaaacagcaa
     8641 agaggaggaa gagcaacatt ccttccactg acgacaatca aaccaagaca attaccggct
     8701 catatattga cacaagcagc agctgtagaa gggtttatag ggattgccag tgaacaagtc
     8761 agttatcccg atcaaatcca aactgtggtt caaaacctgc taggaacgat ccttttagcc
     8821 aaagacttaa ctagtgccaa tgcaatcgcg caaacgatcc gctatcaata tcgagtggtt
     8881 tccttagaag gtgatgtgat gaatgccgga ggatccatga caggtggagc gaataaacga
     8941 ggaaaccaag gcagtttgtt tgtacaaaat caagaattga aacagttgac tagtgaattt
     9001 gaagaagcag ataaacaact ccaagcacaa gaaaaaaaag ttcaggagct gcaacaagaa
     9061 acagctcgtt tagccgaaga acaagaagtt ttgcgtacac gaggcgaaca attacgtttt
     9121 gaagaacaag aagcgacaaa tcagctgcaa aacatcataa atgaacttga acgttttgaa
     9181 aaagaaaagc aaatctcaac ttttgagaca cgtgaactgc agcagtttat cgaagattac
     9241 gaaaagcaaa cgaacgaatt gaaagacaaa caaacagatc ttgaatcgca acgacaaaaa
     9301 atcgacgaag aaatcaaatc gttaagtcaa gaaagcgacc agatggaagc cagaagagca
     9361 caagtacaaa gtcaaaaagc acaagaacaa gcagatcttg ctgtcttgaa agagcagttt
     9421 aatcatctgc agatccagct ccgcggtgcc cgagtccaaa aagcagaggc aaccgaacgt
     9481 caagaagcga tagaaaagca gttggcgaca ttgacagcag acttttctga tcatgaagtc
     9541 acagaagaaa gtttggaaaa acaaattaac gaattatctg ctcagcgaga aacattaaaa
     9601 gccgagttgg caaaagccaa agagcagcga gatagaaggc aaaaggagat cgataagctt
     9661 gaagcagtct tggcagagcg taatcgtgag caaaaagcac gactgtcgga acaatccaaa
     9721 ttagaagtcc aaaaagaccg agctgaaatg attttagata atcatttgac ctatctgcag
     9781 tcagaatatc agctgacatt tgagaaagct tcccaagact atcaagaaac aaccgatatc
     9841 gaagacagta gaacaaaggt ttcatcctta aaagaacaga ttgaaaaact gggaccagta
     9901 aacttaaatt caatcgaaca atacgaacaa gtcagtgaaa gacatacatt cttagcaaca
     9961 cagcgagatg atctgttagc agcgaaaaac caattgtttg aaacgatgga tgagatggat
    10021 gatgaagtgc ggacaagatt caaagaagta tttgaagcta tccgtcaaga attcaaaatt
    10081 gtctttccaa atatgtttgg cggcggtaaa gctgaattag tactgacaga accaactgat
    10141 ttgctgaaaa caggtattga gattgaagta caacctcctg gcaaaaaact gcaaagtcta
    10201 agtttgcttt ctggaggaga acgagcactt acagccatcg cgctgctctt ttctattatc
    10261 cgtgtctgtt ctgttccgtt ttgcatctta gatgaggtag aagcagcgtt agatgaagca
    10321 aatgtgaaac gttttggtcg ttacttgagt gatttccaag atgacacaca atttatcgta
    10381 gtcacacacc gcaaaggaac gatggtcgca gcagatgtac tgtacggagt gacgatgcag
    10441 gaatccggtg tatcaaaaat cgtttctgta cggatggaag atatcaatga agaggcgaaa
    10501 atcaacgaaa cgaaagtgta ggaacgaaaa tgatcaaatt agtagcaatc gatttggacg
    10561 gtacattatt aaatgaacaa aagctgattt cagatgagaa taaacaagca ttagcacaag
    10621 caaaacagca aggggtcaag atcgttctat gtacaggacg ccccttagct gcgatggcgc
    10681 attatctgca agaattagga ctggtagatg aaggtgacta cagcatcact ttcaacggcg
    10741 gtttggtaca aaaaaatgat actggtgaaa tcatggaaaa gaaagtgatg gaagtatcag
    10801 atatccaacg cttatacaaa ctggctcagc aattggatct gccactagat gttttgtctg
    10861 acaatgtggt tttacagctg cctagtgccc caaggaagca atccttgtac aatgtgttga
    10921 ataagctgct gcaattccaa ccagcggata taagcgatat tacagatgaa tttgtgctta
    10981 ataaggcagt aatcgcttat gaccaagaag aattagatcc taaaatcaaa gaaataccag
    11041 cagaattcca tgaaatgtat gagatcatca aaacgagaag tatgcttttg gaatttatgc
    11101 caaaaggtgt tacaaaagct tatggtattt ctttattggc aaaagatctt ggtttgaaac
    11161 aagaagaaat catggcaatc ggtgatgaag aaaatgattt accgatgatc caatacgctg
    11221 gactaggcgt agcgatggag aatgctgtgc ctttcgtcaa agaagcagca gatttcgtga
    11281 cagccagcaa tgaagaaaat ggcgtagcca aagtgatcca aaaatttgta ttagagtagg
    11341 ggggagtcaa atgggatttt ttgataaaat caagcgtgct tttactggcg aagagaaaac
    11401 agaagaagtt gaagtacagg agaagtacga caagggttta gaaaagacgc gtaaaacatt
    11461 tggtgaacgg atgaatgagt tgtttgccaa ttttcgaaca gtcgatgaag acttttttga
    11521 agaattagaa gaaacactta tcggtgcaga tgtaggtttt gaaacagcga tacgaatcac
    11581 cgaagcatta cgtcaagaag tgaaactcag aaatgcgaaa aaatcggcag aagtccaaaa
    11641 tgcgatcatc gagaaacttg tggatctcta tgaagcggaa ggaatcaatg aagtaaatga
    11701 actaaatatc caagaagacg gactaacagt tattttgttt gtcggtgtta atggtgtagg
    11761 gaaaacaaca agtattggta aacttgccca tcagttcaaa aacgaaggta aaaaagtatt
    11821 gatggcagca gcagatacct tccgcgctgg agcaatcgac cagttagttg tctggggcga
    11881 acgcgctggt gttgaagttg ttcgtggcaa tgcaggcggc gatccagcag ctgtcgtatt
    11941 cgatgctttg gaaagagcaa agacagagca ggcagatgtt ttactagttg atactgcagg
    12001 ccgactgcaa aataaagtga acttgatgaa cgaattagaa aaaataaaac gagtaatcca
    12061 gcgggaatta ccagaagcac cccaagaaac acttcttgta ctagatgcaa caactggtca
    12121 aaacgcgatg gttcaagcaa aacaattcaa agaaacaaca gatgtgaccg gtcttgtttt
    12181 gacaaaatta gatggtacag caaagggcgg tatcgtactt gctatccgta atgaattgca
    12241 tttaccagtg aaattggtcg gtcttggtga aggaatcgat gacttggagc cgtttgatcc
    12301 aaatgatttt gtcgttggat tgttcaaagg attactaaaa gaagaataac ccatgtctaa
    12361 aaagcaggag ctgtgacaaa agttgcagct ccttgtttga atgattgttt ttaacatagc
    12421 tgtgtttttg atgccttcat ttttatactt atttgtaagc ggttctcaaa caatttcgtt
    12481 ctttttctta gactttttga ttataattag attcatatga attggaggtc acttaatgat
    12541 gaattttaag caattattat tgcatgtcaa tgcgcgccct tttatcgacc aagctcgatt
    12601 tggtatagaa cgggaaggac agcgggttga tcttgcagga aatctagcaa aaaccgatca
    12661 tccagcaatc tttggcgatc gatcctatca cccctatatc caaacagatt ttagcgaaac
    12721 acaaacagag atgatcaccc ctgttaccga ttctattccc gaattatttc agtatctcgc
    12781 tgctgtttat gatgtgactg ctcgttctat accgaaagaa gagatgatct ggccattaag
    12841 tatgccacct gccttaccgg aaaaagacga agagatcatt attgcaaaat taaaaaattt
    12901 cgaagatgtc ttgtatcgac gttatttagc aaaagaatac gggaagcgaa aacagatggt
    12961 aagcggtatc catttcaatt ttgaatttgg cgatgagttg ctaagaacat tgttcagcca
    13021 tcaggaagaa tttcaagatt tttccgaatt caaaacagaa ctttatttga aaacagggag
    13081 aaactttatg cgctatcgct ggatgatcac ttatttattc ggtgcttcac caatgagtga
    13141 gaagaactac tttttagatg aatcacatcc gcaagaacct gttcgcagta ttcgcaacag
    13201 tgcgctgggc tatacgaatc atccaaatgt gaaagtttcc tatgcttcta tgaaacagta
    13261 tttagcagat atcgagcgaa tgatcgaaga agggaaactt tcggaggaga aagaatttta
    13321 tacaccgctt cgtttccgag gtggaaagaa agtcgcagat ttagcaacaa caggtgttcg
    13381 ttatattgaa ttgcggaata tcgatttgaa tccttatgca agattgggta tcaatccaga
    13441 gcaagttcgg ttcttacaat tattcctgat gtacatgtta tggacagaag aaaaagaaga
    13501 ctgtgaccaa tgggtagcag aaggtacgac tcgaaataac aaagtagcac tagaacaacc
    13561 atcagatcaa acagaatttc atcaagaagg tagagaaatt cttgaaggga tgaagcaaat
    13621 gctggttgaa ttggattggt tggattctct ttatttagta gaggaagcat tgactcaaat
    13681 ggatcatcct gaacaaacgt tagcagcgaa actctatcaa gaagcacagc tatcgagtca
    13741 gcaagaagtt gctgtggcat tgggacatca atattataaa gaaagccatg aacgtcccta
    13801 tcagttggcc ggttttcgcg aaatggaact ttctactcag atatttatgt tcgatgccat
    13861 ccaaaaaggt gtccaagtca aagtattaga tgagtcggat caatttttgc gtctgcaatt
    13921 ccaagaccat gtagaatatg tgaaaaatgc aaacatgaca agtaaggaca gctatatcgt
    13981 cccacttatc atggaaaaca aaacagtaac aaaaaaagtc ttaaaagaag ctgggttccg
    14041 ggtaccagga ggcgcagaat tttcatcaat ggaagaagcc gtaaaagctt atccgagatt
    14101 tgccgatcag gcatttgtga tcaaacctaa atcaaccaat tacgggttag gtatcacgat
    14161 tttcaaagaa ggagctagtc tggaagatta tcaagcaggt ttagccattg cttttcgtga
    14221 agacagttcg gttttagtag aagaatttat gccgggaacg gaatatcgtt tctttgtgat
    14281 cgacggagaa gtccaagcca tcatgttgcg agtccctgct aatgttattg gtgacggtat
    14341 ccgaacagtg aaagaactag ttgaagaaaa aaatagtgac cctttgcgtg gaacgaatca
    14401 tcgtgcacct ttagaattga tccaattagg tgaattagaa caattgatgt tgaaagaaca
    14461 agggttaacg atcgaatcag ttccccaagc caatcaaatt gtttatctaa gggagaactc
    14521 aaatatcagt acgggcggtg attcgatcga tatgactgat gaattttctg aagcttacaa
    14581 aaaaatcgcc gtttctgcgg tagaagcact aggagctaag atcagtggta tcgatttgat
    14641 tataccagat aaagaaatcg atcctacaac tgataaaaaa gcatatggga tcatcgaagc
    14701 aaatttcaat ccagcgatgc atatgcatgt ttatcccttt gcaggaaaag gcagaagatt
    14761 gacgatgaat gtattgaaat tgttatatcc tgaagtattt taaaagaaaa agcttttgac
    14821 agattgtcct atttgatgtc aatctgatca aaagcttttt ttgcatgtaa aaatttatgt
    14881 gttttttcgt tttaaaataa acgatcccag aagctttttt tcttttttgg tttttctttt
    14941 tcagctggcg aagagttttc ttctgggtat tttttttcga tatcaatcac aggttcattt
    15001 actgcttctt ttgctgttaa gagcaatccg aattcctcag gatttgcagt gacgtaatcg
    15061 ttgactacag taaatcggct attgtttttc gttgctagct ggatatattg gttttggaca
    15121 gaaaggggta gagccccatt caagagtatg gaagcatctg gatgtttttt gagttctttt
    15181 aaaaagtttt ctttgttttt tggttggatc atttcagcaa cagtcatact aagatagcag
    15241 cgttccctga atgtccctaa atatttatgc tgttcttcag ggttcactaa aggtgtacca
    15301 taaagtccct tatccaaatg gctttttaca tcatcttgtg ccataaaaaa taccctcctt
    15361 cttctctttg tctttcttac gattatagca tattcccaat aatacctaaa acatatgaaa
    15421 aagcggttgc ttttgttcaa cgattcactt aaaataagaa tgaaaatcat tctcaattag
    15481 aaaggtggat tggatgcgct acggacgact agtagcatta ttagttattt taagtagtat
    15541 ttcattgttc atcggagtat cgaatgtaca attgtcagaa ctgctccagt tagatgggca
    15601 aagcttacaa atcttgctag cgactcgttt gccacgaacg atttgtttga tccttgtggg
    15661 agcgaccagt agtatttgtg ggttgatcat gcaacatttg acccaaaata aatttgtttc
    15721 accaacgact gcgggcacga tggatagtgc gcgtttaggt attttagtgg ctatgatttt
    15781 cctacctggt gcatccttga ttgtccgatc cttgacagca ttttgctttg cttttgcagg
    15841 aacattgctt tttttatccc ttacgcgatt gttcccccag aaaaatcaat tgatgcttcc
    15901 gttgattgga gtcatgttag ggaatatcat tggttcagtt gctacttttt ttgcttatca
    15961 attccagttg gtacaaaata tgacttcatg gcttcaaggg aattttgcga ctgtgatacg
    16021 aggaaattat gagttattgt atgctgttgt tccattgttg ctcttggttt ggctatttgc
    16081 ttaccgattt acgatcattg gtctgggcga atcgtttgca gtaaatttgg gaatagatta
    16141 ccagaagatg cagtttttag gcattggctt agtatcattg gctagtgcag tcatgttgat
    16201 catggtagga agcattcctt ttatcggagt gatcattccg aatcttgtct ctagaatata
    16261 tggagatcaa gtgcacaaaa caattggcgt aactgcttta gcaggtagtt tgttcttagt
    16321 attttgcgat cttgtcgctc gcgtgttgat ttttccttat gaaataccag tcagtgtgat
    16381 cgttggaatc atcggtgggg ctatcttcct ttatcttctg ataagggggc gacgatatgc
    16441 ttaaaaaaaa gccatctatt tttattggta tgcttgttct tgttttattt gttacaggaa
    16501 tgtacttgac gatccataca tatggaaact ggcattttgc atgggcattg aggagtaaga
    16561 aaatttttgc atttatcctt gtctcgctag ctgtcagcag tgccacaatc agctttcaga
    16621 caattacgca taaccagttt ttgactcctt cagtattggg acttgagtcg ttatatgtcc
    16681 ttgtccaaac ggtccttttc ttctttatcg gaggagtgac gatgcttcag cagacaggta
    16741 caggtttttt cttattgaac gttgggctga tggtcgggtt gagtctgttg ttatttttct
    16801 ttcttttgaa aaaaatgaga gcgaatcttt ttttattact gatgtcaggg atgattttag
    16861 gtacgctttt tggtagtgtc agtacctttt tacaagtcat catggatccg aatgaatatg
    16921 atttgcttca aggcagatta tttgcaagct ttggcaatat ccagagtcag tatcttttgc
    16981 ctgcttcttt gatcattatc ggctgctggt acctgttatt tcgatatcga catgtgctag
    17041 acgttttcca tctcggaaat gaacaagcag taaacctcgg gatagacaca gatcgttttg
    17101 aatttttgat tttatgttgt gtcagtcttt tgacagggac atcaacggct cttgtgggtc
    17161 cagttacttt tttagggttt attgtcgcaa atatcagtta tcgtgtcttt tctacttatc
    17221 gtcatggtca gctatttatc ggaagctttc tggtagctat gctgtttctg acaggaggac
    17281 agctcgcagt agagcagatt ttcggctgga atacaacagt gagtgtcgtc gttgaatttg
    17341 tcggaggaat ttattttgtc acaaaattga ttcgcgaaag gaagagtatc ctatgattga
    17401 tttacaaaag gtcaataaac tgtaccatgg aaaatcagtg gttaaagatg tacagttaac
    17461 gattcctgaa aagaagttga ctgcttttat cggtccaaat ggtgctggaa agtcaacggt
    17521 gttgtcgatg atcagtcgtt tgataccaaa agatacaggt gaaatctatt tagaccataa
    17581 cgaagtaaag gcttggaaat ccaatgaatt agccaaaaaa ttatcgatcc tacgacaatc
    17641 gaatgaaata aatgtccaaa tcaccgtgaa agaattagta gaatttggac gatttcctta
    17701 tacgaaagga aaattgacga caaaagacga ggaaatcgtt caggaatcac tggaacacct
    17761 tgggttagaa gaaatagcgg atcaatcgat tgacactctt tcaggcggtc aactccaacg
    17821 tgcgtatatt gcaatggtac tagcgcaaga cacggattat attttactgg atgaaccttt
    17881 gaacaatcta gatatgaatt ttgcgattca aatcatgcag atcttaaaaa aattagtaca
    17941 agaaatcggg aaaacaattg ttatcgttct tcatgatatc aactttgctg caagttatgc
    18001 agacgaaatt gttgcgatga aagatggaca gttattcacg caaggaacga cccaagaaat
    18061 gataaaaaaa gatgtattgg atgaattata cgaaatggac atacggattt gtgagattga
    18121 aggccgcaga ttctgtcttt atttctaaaa ctaacattag gagagaagag atgaaaaaat
    18181 tagtagtagg tatcttgatt ctagcatcat ttggtttagc agcatgtgga aatagcaaca
    18241 acaccagtca ggcagataca aagaaaagca gtacgcaagc tgaaacggca ttaacgataa
    18301 aagacagtaa tggcgatcaa atcgaggtgc caaagaatcc tgaaaaagtc gtcgtattcg
    18361 acaatggttc tttagatacg atggatgctc ttggtgttgg agataaggta gttggagcag
    18421 ctacgagcag ccttcctgag tatctttcct cctataaaaa agtggaatca gcaggtggga
    18481 tcaaagaacc agacttagaa aaaatcaatc agcttcagcc aaacctgatc atcatttctg
    18541 gccggcaaag agattttcaa aaagatctaa gtcaaattgc tccgaccatt ttcctttctt
    18601 tagatgcgaa aaatccatgg gaatctttcc agcaaaatgt cactgcttta ggggagatct
    18661 ttggtaagca agaagaagct aaaactcaat tagaggaatt atctagtgca atcgatcaga
    18721 cgaaaaagaa agcagaagct actgacaaaa aagcactagt cacacttgtg aatgaagggc
    18781 aattatcagc ctacggatct ggttctagat tcggtttcat ccatgattta tttggctttg
    18841 aacaagcaga tgatcaaatc gaagcatcca ctcacggtca aagtgtttct tatgaatatg
    18901 tgctggaaaa aaatccggat atcttatttg tagtagacag aacaaaagct attggtggag
    18961 acgacagcaa agatgatatt tctgccaatg aactggtagc acaaacgaat gctggtaaaa
    19021 atcagcagat catctccctt gaaccagatg tctggtattt aagcgggggc ggtttagaat
    19081 caatgaaact gatgatcgaa gatgtgaatc aagcatttaa atagcataaa taacagagga
    19141 ttaaactctc atactatgta acaataatgt ttcttacatt cttaaatagc aagagcctat
    19201 cctcaaagaa gttatcaaaa accatccgta aaatcgtatt gatgatttta cggatggttt
    19261 ttatttattt gggaaatctt ttttcaacgt gaattcattc tctacatcat acgggttgat
    19321 tctagaatgg ggagtggcaa aaaatagtgt gatccatttt ttcagcatca agatcgcctc
    19381 ctttgatttg aatcaaaggt acaaagcgaa acttagataa aacttaaatt tagatatttt
    19441 tattttgtac cgatctcttc tttaaacgat gttccatctg ctgcttctcg gcaataagtc
    19501 cactagacta gaaaatatga gaagctattt tctgtccagc gttcttatta cttaaagcaa
    19561 atcgtagttg tcaaaatctc ttctaaacga tgttccatca gctgaccttc ggtattagct
    19621 caaagcactg atttcacaac ctctatttag gcatgtgtct cagcaagcgg ttgatagttg
    19681 atgatctgca tttttcgata aaaatcggcg ggaagcttac tccaccgttt taataattga
    19741 tagaaataac tttcttctgc tttgatttta tactcgctta atatcaatcc aaaactattc
    19801 gttttttttg aagctaattt tttataaata tccaatgctt tggttttatc ttcattgaca
    19861 aagagtgttt ttacttcaat ataagaaccc tcgatatttt gtgtttcttc gataaaatag
    19921 atgtttttta tatattcctt catagtgatt cctccaaaga agtcttttat ttgataagtt
    19981 tactctaccg aagagttgtt aataatccgt gaacaaaaag taattctttt tccaatagtt
    20041 gttaaaggtt ttttattttt tatagtattt tttgttattt aagaaaggaa taaggttaaa
    20101 caaataacag gttgcttttt gggtcttttt ccgttcaaga aactgaacga agaaaaggga
    20161 aagttgtctc ttttgtgata agagaaatca taataaacga atgacttact ttccaataaa
    20221 ttgagaacag tcagaaccag acaaattagt tgcatctgat gaataaagtg attattctat
    20281 gacatgaggt gatacagatg aaattagaag tgacaaaaga agcacaagaa gtattgaaga
    20341 ataaattaga agcagacgat caacttttat tagatatcga aaacggtgac ggtccatttg
    20401 cagaaagcca agtcagttgt caattagaca cggcatttcg attgatcatt gttaaaaaag
    20461 atacacaaac agatctaagt ctttattctg tagtggctga tacaccagtt ggaccaatca
    20521 aaatcaaaga cagtgcaata ctttacatgg atgatccatc cacacttgct ttagaaccaa
    20581 cttataatag tttacaatta aaaggaccaa gtggcttgag aaaaggcaac ttgcaagttg
    20641 ttcgaaaaga ctagagggat aaaatgggaa atttggcaat catcagcgac ctacatgtgg
    20701 acatcaataa attcggcgaa ccagagctaa tgcagctttg gcaagttttg caaaagcaca
    20761 aaatcactcg gcttcatcta gccggagata cggctaataa agtagatcgc tgcctagcgg
    20821 ttgtcgaatt tttcgccaat aaaggattat ctacgacatt tcatttcggc aatcacgagt
    20881 tggcagatgt gcccaatgaa cagatgatgg accattttcc agacccacat tttttaaatg
    20941 aacggtatat tgctttaaat gagcagaccg ttcttttagg gatgaatggc tggtacgact
    21001 atcagttttc cgaattgaaa gattacgagg caatcgtacg aatgaagcgt ttgtattggt
    21061 atgatcggtt catccagcgt gggaaaacag atctcgaggt caatgcagaa atgcttgcag
    21121 ctgcaaaaaa tttgctagac cagctggaag caaaaaaact tgaggtgatt ttatccactc
    21181 atttcgttcc taaacgggca tttatcatgt atcaatctgc tccttatgag cgttggaaca
    21241 aattaaatgc atttttagga tcagaatcat tcggagaatt acttgaccgt tattcaaata
    21301 tcaaacaagt agtttttggc catacgcatc gtcgctttga agataaatcc atcggtcaca
    21361 cgatttacag ctgcagacct tttggttatt attatgaatg gcagctgacc cgagattttg
    21421 tcttgaaaga acagttgcta gacagctatg atcctatgaa attacgtgtc ctcttgcgtg
    21481 cccatcaccc agcttttcag gcctatcaaa cacaacattt gcaagaggaa tttgaacaag
    21541 cgatgacaat cgttccgtat tgagacagca ttagtaatgt gtaaaagaaa ataccttgta
    21601 aaacataagg ataagtaaga gcagtagatc catttatcgt ggatatgctg ctttttttgt
    21661 ggaaaataat cagcgaatgt ttatttgatc ggatgatttg taactcgcag agtactagat
    21721 gcaagcgttt attctttttt tgaacgataa tcacaaaaaa aaatataata tacatttttt
    21781 atttaattat aattaatatt gattgaagtc taggcagtca agtcacattt tgatagatag
    21841 gagggggact tacgaattat tttaaaggct atgacagctg tatttctaga ttgtgtttgc
    21901 atcaagttgc aataaaaact agaaatcaaa tcgggctgat aatcatttat aactaatcga
    21961 tacataggac caaatatagg tctatgttct tttatttcat aagtatcgta aaacagaatt
    22021 ttgctatgga ttaatccaat cgactgatat gtttagtaaa taaagaaaga ggaatgtcaa
    22081 tgatgaaaaa agaaatcgta ttaacagcag caacgatgtt attcagcatg gtagcaagta
    22141 cgacttttgt ttctgcaact gaagtatacc caaaggaata taacacagaa ggaacgatta
    22201 catttgaagc aggagatgag ggtgtaactc ctcctgttga tccagagaat cctgatccca
    22261 ataaacctgt cgatccatca gatcctccaa gccctggaac aggaggggca ttatcgattg
    22321 attatggatc aaaattcaaa tttgggactc aaaaaatttc tactgcggat aaaacgtatt
    22381 atgcagctgc tgatgtgatg aacgatgggt ctagaaaacc gacatatgtc caagtgacag
    22441 ataggagagg taccttatct gggtggaaat taagtgtctc tcaaccagaa cagtttaaaa
    22501 ctgccagtgg ggatgaacta gttggagcac agctgaaatt tacgaaaggg caggctgttt
    22561 ctttggttga tccaacatat atgccgcaaa cggtgaattc tgaattaacg ttgacaccgg
    22621 gaggaaacaa tacgttagca attaatgcta aatcaggaac aggtgtgggg acttgggtct
    22681 atcgatttgg tgcaaatgaa aatgaaaatc aagatgcggt acagctttcg gtacccggta
    22741 aatctgtcaa gttagcgcaa caatattcca ctaaattagt ttggacatta gaagatacac
    22801 ctaacaacta gtttactgaa aagaactgtt aaaaataaat acgtagtcat attgtcttgc
    22861 gaggtaatcg agcaaaaatt gtcaaaggct aggctaaatt tgtttggata cgaatagttc
    22921 atctaggaat ttaacgatca ggaggaaaag atatattgaa aaagaggagg atcgtgctca
    22981 ttttaagcag tctcttagtg actaatatct ttccgatcat agggtactct ttggaattga
    23041 atcagttgga gcatggaaat agaagagacg aagaaagcaa actcacttcg acattcgaca
    23101 gtttaataga atccaaccag acacaagaaa gcagtgagga tttaagtatt tcgagtagtg
    23161 gagagaccac tgacaacgta gaagctgagg gttcggacaa acaaggactg gaagatgcac
    23221 caatttctgt ggttgagctg cctgacgatc ccaatattat ttctatcgat aaaatttttc
    23281 aaacacctat cggtactagc acgtccatat tggaaaatgg gaagttactg cagttgaacc
    23341 cagcagaaag gtctcaaaga ggagctattt ggtcaaataa gaagattagt ttactctcgg
    23401 attttacatt taaatcttat ttatacttag gaaatcaacg agatagagcg ggagatggca
    23461 tgacctttac attgactggc gatcctagaa tggctagtaa tcccagtcaa gttattggat
    23521 cgccaggtat gggaattggc tcatattcta ctcgttctgg ccaaccttac attgcgaatg
    23581 cgctctcgat tgagtttgat acgtataaaa atagtggttc ttctgatcga atggatcgag
    23641 agataactgc tgataataga gggcatggac atgtcgcttt tgtcacgcct aaaccaaata
    23701 ataataatta ttctggagag catactggcg tgacggtagc tcctacattt ctttctaatg
    23761 gcacatggcg cttgttaata attaaatggg acgcggagca aagaagtctt acttatgatt
    23821 tggaaggtgt cggtcgctct tctcggatta tacctgatct cattaggcag tttggttcta
    23881 cagaagtaaa ttgggggttt acttcttcaa caggtacttt ttttcaagaa aatgctgtcg
    23941 ctgtcacgcg acttccatcg aatgtcacat caacagctga tgtaaaagtc aacgatagtg
    24001 attatggggc catcgctgaa gctggaggaa acgataagat aactatcaga aatacattga
    24061 aggtcgaggg ggattttctt gaatataaga aaatgcagac tagtatcgac ctcccacaag
    24121 aattaactat tcccgagcat atcttgatca atggaaatca aattgatgcc aaagatatct
    24181 tgataaccgg aaatcgagtc acgctaaatc tgacagatta ttttattgct ggagatacgt
    24241 taataatcga attggagaca actttgcagg aagtaattcc tgaaaaagta ctcactatgc
    24301 attttgaata ccttgaaaac gagaatttga ttgagcaatc gaatgaaata aacctaaaga
    24361 tcgcaaagct gaaggagata gatatccatg tttattataa agataaagac agtcagattg
    24421 atattgctga aatgaaaaca atcactggga aaataggaga gaagtataaa gaaactccta
    24481 ttgatgttga agggtatgtc tttgactcgg attcagcgaa tacagaaggc atcttctccg
    24541 aaaatacaga ggatatctat ttttattaca gaaaaggcaa actttatctc tcaactgctc
    24601 ctcaatatgt caattttgga aaacataaaa tatccagtag accattaagt gaattcggac
    24661 agttttcagg tgggttgaaa attatagatg aacgggcttt tggaagttgg aaattacaat
    24721 tgaaacaaga gaaagttctt acaaatggag attttgagct tccagaagct ctttcctttg
    24781 ttacctctga aaatacaacg gttataggga catccgctgt tacgattttc gaaagtgatc
    24841 aaaagggtga aagcgatttg tcctctcttt tagattctac agctcatcgt ggaatcagaa
    24901 tagatattcc tgtagaaaac caaagaatcg gaacctttga ggggaaacta tcttggatac
    24961 ttgcagatgt cccaggaaat taacaggaag agaagaatat aacaaaatta ttgagtttca
    25021 agaagggcac aggtaccata ggctcttgtg tcccacagag acaagatttt gttatagtct
    25081 tctcttacat atgattaaag gagcgatttt cttgagacca aaacaacaat tatttataac
    25141 actcctagtt attttagggg cactttttaa caatatatca gtgtcagcta gtgcatttaa
    25201 tttttcagtg acgccgataa cttcagagaa tcaaatcgac aagagaaaaa cttacttcga
    25261 tcttcaactt gatccggatc aggaagtaga agtaaaagcg gaattgagaa atgatacaga
    25321 aaaagaagtg aaaatcgata tttctgtgaa tagcgcaacc accaattcaa atgtgatggt
    25381 tgaatacgga aaaaacgaga ttgaaaaaga catgagcctg atctttgatc tggtagatta
    25441 tgtgagctat ccacagatag tgacgctaaa acctaaaagt gtccaaaccg ttgcttttca
    25501 tgtcaggatg ccgaataagc gatttgatgg tgtattggct ggtgggatca cattccaaga
    25561 aatccagacg gaaaaggacc aaacagaaac taaggatcaa agcttatcga ttgagaatga
    25621 atacacctat attattgctt tgttgatgcg acagacactg aacgaagttg ctccgaacct
    25681 tactcttcat taagttaaac cagatcaaat caatgcaaga aatgtcatct tagcaaacgt
    25741 tcaaaatgat caaaaaacct atatcaatca agtagttatt gaaacaaaaa ttacaaaaaa
    25801 aggacattct gaagttttat atcaagagga aaaagaagcg ttgcagattg cgccgaatac
    25861 aaatttttct tttccaactg ctttaaatgg tcaaccgcta accccaggtg aatatcacct
    25921 tacaatgact gttttaggaa atgaaaatga aaatggaaaa ttttcgagaa aaaaaggaaa
    25981 tactacgatc aactatacca atcaatgggt atttgaaaaa gattttacaa tagacgaaaa
    26041 agtagccaag gaattgaaca cgacggatgt tacgattaaa aaagacaata attggattta
    26101 tatcttagta gcttcacttt tcttattgtt tattttatta tgcatacttt
//
LOCUS       DAZABD010000042        25644 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000042 DAZABD010000000
VERSION     DAZABD010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.042"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(153..551)
                     /locus_tag="ACHALG_001772"
     CDS             complement(153..551)
                     /locus_tag="ACHALG_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFN2587157.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(548..1321)
                     /locus_tag="ACHALG_001773"
     CDS             complement(548..1321)
                     /locus_tag="ACHALG_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFN2587158.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1318..2001)
                     /locus_tag="ACHALG_001774"
     CDS             complement(1318..2001)
                     /locus_tag="ACHALG_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFN2587159.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2168..3055)
                     /locus_tag="ACHALG_001775"
     CDS             complement(2168..3055)
                     /locus_tag="ACHALG_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN2587160.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3052..3921)
                     /locus_tag="ACHALG_001776"
     CDS             complement(3052..3921)
                     /locus_tag="ACHALG_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN2587161.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4058..5344)
                     /locus_tag="ACHALG_001777"
     CDS             complement(4058..5344)
                     /locus_tag="ACHALG_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN2587162.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5617..6195
                     /locus_tag="ACHALG_001778"
     CDS             5617..6195
                     /locus_tag="ACHALG_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFN2587163.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6195..7124
                     /locus_tag="ACHALG_001779"
     CDS             6195..7124
                     /locus_tag="ACHALG_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN2587164.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7121..8857
                     /locus_tag="ACHALG_001780"
     CDS             7121..8857
                     /locus_tag="ACHALG_001780"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFN2587165.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(8898..9857)
                     /locus_tag="ACHALG_001781"
     CDS             complement(8898..9857)
                     /locus_tag="ACHALG_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFN2587166.1"
                     /translation="MTYTHLTPTELVMIEAYFNQSHSVSKVAHLVQRSRQTIYNVYRF
                     LKSGGSAISYYEQYKKNKRNCGRRPIVLPDGQQEYIQKKVAQGWTPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDPHDLPMKGKRKPNGHKEKRGKQAFRRSIQDRTTDYIQFNHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPAGRQAIDIENRLNQWFQSVPKHLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNK
                     VSETFIQSVASKRNHIPRKSLHYRTPLEVFLSYVGKDDLSSLI"
     gene            complement(9985..10821)
                     /gene="pheA"
                     /locus_tag="ACHALG_001782"
     CDS             complement(9985..10821)
                     /gene="pheA"
                     /locus_tag="ACHALG_001782"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFN2587167.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(10818..11327)
                     /locus_tag="ACHALG_001783"
     CDS             complement(10818..11327)
                     /locus_tag="ACHALG_001783"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFN2587168.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(11320..12615)
                     /gene="aroA"
                     /locus_tag="ACHALG_001784"
     CDS             complement(11320..12615)
                     /gene="aroA"
                     /locus_tag="ACHALG_001784"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFN2587169.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREIGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(12628..13719)
                     /locus_tag="ACHALG_001785"
     CDS             complement(12628..13719)
                     /locus_tag="ACHALG_001785"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFN2587170.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(13736..14902)
                     /gene="aroC"
                     /locus_tag="ACHALG_001786"
     CDS             complement(13736..14902)
                     /gene="aroC"
                     /locus_tag="ACHALG_001786"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFN2587171.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(14904..15968)
                     /gene="aroB"
                     /locus_tag="ACHALG_001787"
     CDS             complement(14904..15968)
                     /gene="aroB"
                     /locus_tag="ACHALG_001787"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFN2587172.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(16001..17023)
                     /gene="aroF"
                     /locus_tag="ACHALG_001788"
     CDS             complement(16001..17023)
                     /gene="aroF"
                     /locus_tag="ACHALG_001788"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HFN2587173.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(17682..18656)
                     /locus_tag="ACHALG_001789"
     CDS             complement(17682..18656)
                     /locus_tag="ACHALG_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HFN2587174.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(18637..20520)
                     /locus_tag="ACHALG_001790"
     CDS             complement(18637..20520)
                     /locus_tag="ACHALG_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HFN2587175.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(20538..22763)
                     /locus_tag="ACHALG_001791"
     CDS             complement(20538..22763)
                     /locus_tag="ACHALG_001791"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFN2587176.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEEKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            22878..23912
                     /locus_tag="ACHALG_001792"
     CDS             22878..23912
                     /locus_tag="ACHALG_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN2587177.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(23979..24992)
                     /locus_tag="ACHALG_001793"
     CDS             complement(23979..24992)
                     /locus_tag="ACHALG_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFN2587178.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKHLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(25070..25258)
                     /locus_tag="ACHALG_001794"
     CDS             complement(25070..25258)
                     /locus_tag="ACHALG_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFN2587179.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
BASE COUNT         7359 a         5541 c         4591 g         8153 t
ORIGIN      
        1 agcatatttt gaaaagaaag tctgatgctt agagtagaat ttcataaaaa tttactattt
       61 cataagtatt cacgagaaaa atacaaacaa aaaagcggaa ataacgctca tccgccttct
      121 tgtttgtgtt tatttcctct tcaaacgctt gtctatgatt cagacttttc tctatcgtta
      181 ttttcatcta aaagttcttg cattccgata atttctcgat attgatgaat cttagtgata
      241 ccaatgactt tgtctacatc aatactaaaa caaataccat tacctggaat atcgatgttg
      301 atgccttgac caatcgcatc cattacttta ttcgtcaaac tatcgggtac taatgtcaac
      361 acaatatctt tttctggcga aatgtccaaa cctaaaaatt tccctgaatc atgtacacca
      421 gaacctcttc catgtaagat cgttccgcct gctgcacctg cttcttttga aaagtcgata
      481 acatcttcgc ccattcctcc gtcaacgatc gtcacaatca tgtgaagatt caatgctaca
      541 agatctttca tttgctttcc cccaattttt gcaatctttc tttttcgatt ctgtcttttt
      601 catcttttcg tttataaata tatcctagta ctaaagtgga taatattggt gtcaatgcaa
      661 tcattgcaac cacaccaaac ccgtcaatga tcggatctct tccttcaatt tgtgcagcga
      721 ttgccacgct cattgataaa ataaatgtcg aacacatcgg gcccgttgct actccaccgt
      781 tatcaaaagc cattgctaaa aagatcttgt ccacttttcg tcctaaaata aatgctagcc
      841 cgtatcctgg gatcaagaaa taataaagag agaatccgac gagcaaacgc cacatagaca
      901 atcctactgc tgcgccgatc ccaattgaaa ttaccatcag catgattttc gatcggattc
      961 tcccctcgct caattcttca acctgtttga tcattacatg aatcgccggc tcagcaaacc
     1021 ctactaagaa ccccattaaa aagccaaggg gaaccagtat ataattgtaa ggcaatcccg
     1081 caatagcact gcctaaatgc tgtcctaccg gtacataagc catattgact ccgtgcagga
     1141 aaaggatcaa cccaatagtc gttatcaaga aacctttcat aatgtcaaag aaacgctttt
     1201 tgcgcatacg aaaactaatg atattcatta aaacaaatag gattacgata ggtgccactg
     1261 ccaaaatgac ttccatggta acgatttcga ttccatctat cacttgctgc cagaaactca
     1321 ttggaagatc acccccaaaa tcatcacggc aagaatcggc cctaacgaag caataccgac
     1381 cattccaaaa ctgtcatttt catttttctt ttgtttgatc atactagtca tccctccggc
     1441 aagggccaag ataaatggaa cagttactgg accagtagtc actccccccg aatcaaaagc
     1501 aatcggcata aattcggaac tggtgaaaaa tgaggcgatc aaaacgccta tgtagcctat
     1561 agccatcaat tgataatagg atagcttgaa tacgacccga agcagcgcaa aggctagaaa
     1621 tactccagtg ccgacgctga cgatcccaat cagcaacact ctgccaatct ctccgtcaga
     1681 tatatcatat acttgttgtc ccaaaacttg cacggaaggt tcagcaatcg tgatcacgat
     1741 cccgatagca aatcccaaac tgaccaaaac ggtcaaactt ttctttttga tcatatattt
     1801 ccctactaga tctccgactt ccatcataga ataatctgct ccaaacaaaa agagcgtcat
     1861 tccgatcatc atgattgccg caccaaccag aaatgaagcg aggtcttctc cttctaacgg
     1921 tgcaaaaaca aacgttaaaa tgacaatcag gacagtcatc ggcaatatag cagcaatcac
     1981 ttctttaaaa ttttttagca tgtatcctcc tcctttactc tttaagttta ccttaaaatg
     2041 aaagagtttt cgagcacaaa cttaaaacgt tatcatttaa tttttctcaa cataaaataa
     2101 cacacgtccc aaaagtatct ttttacttgg aaaaatgact ttgaaggaac gcgcttattg
     2161 tgttttttta ttttacagag ccgatcattc cttgaacgaa ttgcttttgc aaaacaaaaa
     2221 atacgatggc agttggtaaa gtagcgatga ctaatccagc catgatcatt ccatagtctg
     2281 gtgtatagga agcacccata gcagataaaa caagcggaac cgtccgttta tcaggagatt
     2341 gcaaagctac taagggccat aaataattgt tccagctact catgaatgtg atgattgcag
     2401 ccgcggcata ggtattttta gccgtcggca tatacactct aaaaaaaata cttagttctt
     2461 ttagaccgtc taatcgagcg gcttctacta gatcttttgg aaaagattta acattttgtc
     2521 gaaagaagaa gatcaaaaaa gcagtgctaa cagatggcag gatgacaacc aagaaactgt
     2581 tgattcccaa aaccgttccg ttcaattgtg aaaacattcg atataaaggg atcatcaatg
     2641 ccgcaaaagg aaccatcatc gatagcagta gaatattgaa aattaggtct tttcgtttac
     2701 tttgataaat ctcaaatcca tatcctgcca ttgacgaaat cagcaatgca aacacagtgg
     2761 tcaccaaagc gatcatcgct gaattccaaa gggaacgagt aaaattcagc tcattggaaa
     2821 acaaattttg aaaattgatg atcaagttac tgccaatctt tatttttccc gatgtgatat
     2881 ccacgggtgt attcgtcatt cccaaaacca tccagacaaa agggaaaatg gaaataaatg
     2941 ataggatgct taatgctgta tactttaata ctgacccaat tgtcgaacga agttctaatg
     3001 cttgtttttt gacgttgggg cgttcgattg tttctttttc taccatattt ttcatcgact
     3061 ttctcctcct actttcgttt ggattatcga caatatcaca ataaaggaaa caataacaaa
     3121 ggaaactgct gccgcatatc caaaattagg cgtgaattta taactcaaat tatagatgta
     3181 ttgagatagg gtggtcgttg catttgctgg tcccccgcca gtaatatttt gtacttcatc
     3241 aaaaagctgc aatgtcccga tcgtcgaagt aatggaagta aacaaaatga ttggttttaa
     3301 atttgggatc gtcactttga taaactgctg ccatttagaa gcaccgtcaa tagatgctgc
     3361 ttcataaatc tccgaatcaa tattttgcat gcctgaaagg aaaaaaatca tattgtatcc
     3421 agtccaacgc caagtgatcg aaagaatgat cagaactctc gcccaaattg gatgagtcaa
     3481 ccagccaatc ggtgtgtcaa tcataccaat gcccaacaac aagttgttta caaggccaga
     3541 ttgagaaaat agacttttca tgatcagaga ataactgacc atcgaagtga tacatggaag
     3601 aaagatagcg gttcgaaaca accctttata tttcaacttt ttatcattta aaaggttgga
     3661 aataaaaagt gctagaaaca acatgatcgg tacttgtaca agtaaataca ggaatgtatt
     3721 aaaaaaagct tttttaaaag ttgcatctgt caacatccgc tgataattac ctaacccgtt
     3781 aaatgtcata ttgacagggg gaccactttg aaaagacgtg atcaaagcca aaaccatcgg
     3841 aataaaaacc atgattgata tcaaaatgac tggcaagatt aaaaaagcat gcccgttttt
     3901 gaataaatct ttctgcttca ttcttttccc tccaattcta tcattgatta tctacatgaa
     3961 aagacaagaa agacaatatc tgtaaggcag acgtgatctt ttttgtcttt tcgtttgtct
     4021 gatgaaaaag cgaagaaatt cactttttta ccaccgatca attagccaat tgtgcttcta
     4081 cttgtttttg tgtatctgct agtaccttgt ctgtctcttc tccattgatg attcgatgaa
     4141 gagcttcagc tacgactgct tcaattgcat aggtctcatg tccgtaattc acttctggta
     4201 tttcagatgt ccatgttgaa aaatcgtcaa agatcttttg accactgtaa aattcagaag
     4261 cttcttgata agcttcttgt tcattcgcgc taagcatcgt agatactagt ccgatttctt
     4321 tcgcaagagt gcccatcagt tcttcattcg aagcaaacgt tgcttctagg aaatctttcg
     4381 ctgaatcttc tccagctact cctttgatca cataccagcc agcgccgcca aggttagaag
     4441 cttgcgcttt ttgcagattt tctggaagtg caggtattgg tgcaatcttc cattttccag
     4501 attggttttc tgctccttgg atcgttgaag aataccaagc gccagttggg ctagaagcta
     4561 ctgctcctga ttgtatcgca gttactccag cattccagtc agaggtttgt tcgactagat
     4621 cttctttcaa aagagtggca aacaattcaa gaccatactt taaagatgca ttgtcttgga
     4681 tcgtgactgt ctccccatct tcagatgtat accattctcc tgcctgttgc ataatcatcc
     4741 ttactcgacc aaggtcagaa ggattgactt cagcgatttt ctttcccgtc ttttctttga
     4801 catctcgcgc tacttgaata tactcgtccc acttcagatt ctccatatcc tcttctgtat
     4861 aacctgcttc tgccatcaga tccgtacgat agaagtttgc tgttacgccc gaatcgaaag
     4921 gaacaccgta tattttatct cctactttgt ttaccgcaaa tttatacggt gcaaaatgat
     4981 cttcctttac aatagcagac aaattagcga atgcatctgg ataagaagtc aaatagcctt
     5041 gtatcctata atcttcaatc aagacgatat tgggcaatcc ttctgtattc ccgctcgcta
     5101 aagcggtgtt cagtttctga acgatatcat cctgagacat cgtaacaact tctatctctg
     5161 tttcttcgtt ctcataaact ttttttgctt cattcactgc tttgatattg aatgtctcat
     5221 cccacgccca gacagtgact ttttcttctt tgtctgctgt actactagca cttccgcatc
     5281 ctgttaagaa acctgatgct gccgctaaac aaacgactcc agcaattaac tttctgatgt
     5341 tcatttttat ccgctccttt tccatccatt agttgtttac gtttgctaca ttcatcatac
     5401 caaaatttga aaacggtttc tttattattc agatacttca aaaatttctc ctaaaataat
     5461 aaaaaaatac aaatcaacta tgacaaaagg aataatctta gcatctagac caaagatcga
     5521 aatggtgagg tttctttaaa ataaaaatat ccttgcattt tctcctagaa ataagtattt
     5581 ttaaattaca agactattct taaggcgggt aaaaaaatgc caatacgcgt aattcttatc
     5641 gatgatgaac agacgatttt agatggaatg aaacagttga tcaattggtc tgagcacggg
     5701 tttcaattaa tagggacttt ttccagtccg ctgaaagcgc ttgatttttc tttggtacat
     5761 gcaatcgata tcgtggtcac agatgtaatg atgcctgaat taaatggaat cgaattatcg
     5821 cggctattaa aacagcaaca acctgaaaca cagatcctta tcctctctag ctatgatgaa
     5881 tttgatatgg ttaaagattc ttttaaagaa ggcgtcgctg attatctttt aaagcctcga
     5941 ttgagtcctg atttttttct aaattcctta actgccgttt cacaaaaaat caaaaaaaag
     6001 cccaacaaaa gcccaatcac caatcgttat gagcagatct gcgagaatct ttcacagcag
     6061 attgctggac aaactcgtcc attgcttgac cctcttcttt ttgaacacac cttgtttttc
     6121 tgttatatac cgaaaaacgg ccattcagtt caaaacaagg ctctaaacaa aaacagtcga
     6181 tcacccattc attgatggat agcgacgatt cgtttactat ttatcctgtt tctacctttt
     6241 cggaagaatc cggctatgta atcaatactg attccaaaga tacacttagg aagtttgttc
     6301 aaaagtttcg tcaagaagtt tttccaggtg atttttttgt tgtttctgat tcttttgcaa
     6361 tccaagatat caatgaaata ttccaagaga tcagaaattg caccaaagga caacgtttct
     6421 atcataaggg aaagaacctt gcttatcagc atgaacttct tccgttatta accaaccacg
     6481 agcagcaagc aagtagttat ttaacaaaaa tcgttcataa agactacgct ttagctgtca
     6541 aagaacttga gaatttcttc acgacgatat tggaacagcc tattgatcca gcttatttga
     6601 aacatgaggc aatcaatcgt ttctatgcat tactcaacgc tttggctgac gagcatgttg
     6661 ggaatgaaga actttctttg ctcaagatcg cccttccttc tttattagcc gatgctgagt
     6721 atttcgaaga tttttctatt cttgtcaaag aaacacttga gaaactcacc cacttttctg
     6781 caaatgcaaa aaaagacaat ttcgatatct tgactttgat ctacgagttc gtccaggaaa
     6841 attatgagaa agaaatcaat ttacaacttt tatccgaaaa atatcatttt tcatatagct
     6901 atctctcagc catctttact gaaaaatttg gtatcaactt ttcaaaatat ctaaaaaaag
     6961 tccgtatcag taaagccaag ctctttttga cggaaacacg gcttactttg acggaaatct
     7021 gtgagaaagt cggctatacc gaactgggtt atttctccag agtttttaaa gaagagacag
     7081 gaatgacacc ttctcaatac cgtaaaggaa cttttgtaaa atgagaaaaa aaatcaaaga
     7141 gcatgaactc tttgccaaac ttctgttcat tttgttcatc ggtctttttc tgcaagccat
     7201 cgtcatttct ttgtttattt atcatcgatc aagggatgcc tatatccagc tgttcaatca
     7261 atcgaatgat gtggtactaa aaaagattca atctgaattt gaaagtttga atgatacaat
     7321 cgagaataca ttagccgctt ttgattctaa tccagctgtc aaaagctatt tttccaatga
     7381 tccagcccaa catatggaac agcttcagca actaagaaca atccaaaaaa tgaatgactc
     7441 tctttcaaaa atccatccaa tgatcgatta cgatgtattg atctttggag aaaatggaag
     7501 aacttttgtc ggaaacgaca tgctcactgc cgtatctgcc gattcttttt ttcaatctgc
     7561 gattgctcag agagtgaatg aacgtgctgc tgatacacaa atgctttttg cacaccacgg
     7621 attgactctt cgcgataaga aagcacccag cgtcttcttt gtcaaaaaac tgaaaaatac
     7681 attgaatcac gtttacggat atgctgtttt atcgatctca tccaagcaac tagcgaatct
     7741 ttttcaatct gtagtaaatc ctgagatatc gaagatctcc ttcatgaacg aagaacagac
     7801 gatcatcgca tcaaatgaag aaaatacaat tgggagccga tcaatgattt tcaaaaaact
     7861 aacagtaggt gaaacaacga tttcaaatgg acagcagttg acccgtcttc ctctgtaccg
     7921 ccaggattgt acattactca gtcaagtcga tcttcattct ttatctaacc aaatgggcgt
     7981 gatttttccg attattttgt ataatatcgt gacacttgct tttgctggct cattggtatt
     8041 tatgtatttg gatcgacaca caaaatcgat ctaccgcttg attgattcgc taaaacggct
     8101 agaggaagat ggcttaaaag ctactgttcc tgtacaagga agccatgaag tccaattgct
     8161 agggaatacg atcaatcaat tgctaagtaa tgctcagcat cattatgaac agacgatcca
     8221 agacgaaaag aagaaacgtt cgcttgaaat ccaaactatg caagcacaga tacagcccca
     8281 ttttatttat aatacgctga ctagcttgaa atttttgatt tggcagaaaa aaacagatga
     8341 agcagttcaa ggaatcgaca gtttcattac tttactaaga agtacgattg ggaaaaaaga
     8401 agaagtgatt ccagtcaagg aagaaatcaa aagcgtacaa agttacatcg atcttttgag
     8461 tatgcgatat ggcgaaagaa tctcggctaa aataatgatt cctgatgaac tgcagtcatt
     8521 atctatgccg aatatgatcc ttcaaccaat tatcgaaaac gcctatcttc atgcttttca
     8581 caataaaacg caaggattca tcacggtgta cggcaaaaca aaaaatcagc ttcttttatt
     8641 cgaagtgatt gataacggcg atggcttcga tactgaaaat accaaacaaa aaaatgatca
     8701 tttttcagga atcggcatca gccatgtaga tgagcggata cgcctaatgt atggcgatgc
     8761 atatggctta acgatccaat cagtgattgg gacaggaacg actgtaacga tttgtctacc
     8821 gcttcttcct aaaaatcaat cagaatatca aaaataaaga atagccaagc gctgattttt
     8881 ttattttatt tcgtttgtca aattaagcta gacaaatcat ctttacctac gtaactcaaa
     8941 aatacttcca atggtgtgcg atagtgtaat gattttctag gaatatgatt tcgttttgat
     9001 gccaccgatt ggataaaggt ttcggatacc ttattaaaat ccatagcttt atataagcca
     9061 tctttacgta ataagccatt cgaatgttca tttagtcctc gttgagaagg tgttcctgga
     9121 tcagcgaaat aaatcgaaat atcattggta ttactaatct ttttccaatt cgagaactct
     9181 tttccacaat caaatgtgat ggatttaaac aagtgtttcg gtacagattg aaaccattga
     9241 ttcagtctat tctcaatatc tatagcttgc ctaccagcgg gtttcaaggt gatgatcact
     9301 ttagataatc gctcaactaa cgtgatcacc gcacttttat gttttccacc gacaatggtg
     9361 tcaccttcta ggtgaccaaa ctcatgattg aactgtatat aatcagttgt tctatcctga
     9421 atcgaacggc gaaaagcttg ttttcctctt ttttctttat gtccgtttgg ttttcttttc
     9481 cctttcattg gtaaatcgtg agggtcaaag gttttatctt taaacatccg atatagtgta
     9541 cgtaccgaac aagcaatagg aaactctgct cgaccaataa ggacatctgg agtccaccct
     9601 tgagccacct ttttttgaat gtattcttgt tgcccatctg gtaacacaat cggacgtctt
     9661 ccgcagttac gtttgttttt cttatattgt tcatagtagc ttattgcgct tccaccagat
     9721 ttaagaaaac gatagacatt ataaatggtt tgtcgagaac gttgtaccag atgggctact
     9781 tttgaaacag agtgtgattg attgaaataa gcttctatca ttacgagttc agttggtgta
     9841 agatgggtat aggtcatttg cactcactcc ttataattct ttcgacggaa ctattttgag
     9901 tgtagcacaa atgacttttt tagttgtcta gcttaatttt acaatcggcg ttaagttaaa
     9961 taaatcaatc agtattcttg actttcatct acgcgtctct tctactgtca gcactggata
    10021 acagcccaat atttgacttt ctcctccgag catcttgatt tcttggagcg cattctctac
    10081 taatgtcatt ggacggtcca acaacaaatc gatcacgaaa aaatattctc ccaagctagt
    10141 tttcaatggt cgggattcaa tcttacttaa gttgatttcc cgccagccaa aagctgccag
    10201 cattttatga agcatccctg gccgattagc aggtagtgtc aagatgacac tcatttttac
    10261 tggagctgac tgctggctgg tcatctttcg gtcaccgacg atccaaaaac gtgtctggtt
    10321 caactcatta tcttggatat tctcagctaa gatctccaat ccaacatgct gtgctgtttc
    10381 taatgaagca atcgctgctg cgtcttcttt cggatgttct gctacataca ttgctgcagc
    10441 agttgttgat tctgttgcta ctaaaggaac attaggataa tgcgtttcta aaaactgttg
    10501 tgattgtgct agtgcttgtg gatgtgaaag gatttttgta atctttgttg ctggattgcc
    10561 taaaagctgc tgcttgatag gaagaacgat ttcactttta acttcgactt ctggatgttt
    10621 ggaaagaaga tcgattgtgt gatgaacagc tccttccaat gaattctcta caggaacgac
    10681 agccagatcg acttgtttgc gaaacaaagc gcgcaaacaa acagggatgg aagcatacgc
    10741 agtcaattgt tctgtaggaa atagctgaca agccgcctga taagtgaatg aactttctgg
    10801 ccctagatag ctgacgttca tatgcttttg atcctttcta taatggtatc gatgatcttt
    10861 tctggtgttt gattggtcgt gttgattcga tgtgtcgcac tttcttcata ccaagaaagt
    10921 cgttgtgcca ataatgcttt tatttcttct cttgtttttc caagagctaa aggtcgaata
    10981 gacgtttcat cttgttggat acgatcaact agacagtccg cttctgcctc taagtaaaga
    11041 acaatcggct gtgtttttaa aaaacgccga ttttctgcac tttggacaat ccctccacca
    11101 gtcgcgataa ttttgtcttc atttgacagc tttcttaaaa tatctgactc cacttttcga
    11161 aagctttttt caccgtaata gtgaaaatat tcggcaatcg acaattggat atgttcctca
    11221 atctttgtat ctaaatctac atactctttt tgtaaacatt gagctaaccc tttaccgatt
    11281 gttgtttttc cagcgcccat gaaaccaatc agcaaaatgc tagccatgag cttctcctcc
    11341 tgacacaagt tctgctaaat cgtcaaaaaa tgccggataa gagattgaaa cagcttcagc
    11401 tccttctagt tcgcaaggtc catcagctat caaagcagct acttgcagca tcataccgat
    11461 tcgatgatcg cctctgctgg acacttttgc gccatgcaga ggtgtcggcc catggatgat
    11521 cagaccgtct gctgtcgctt ttatctttgc tcccattttc tgtaattctt ctgccaccgc
    11581 atcaattctg ttggtttctt ttacttttaa ttcttctgcg tctttgatga tcgtgattcc
    11641 ttctgcttga gtagccacta aagctagaat gggcaattca tcaatcagac gcggaatgat
    11701 ttctccacca atcgttgctt tcttcaaatg actcgtcttg acacttaaat cagccgattg
    11761 gttctgttca ttgtgattcg tctgggtaat cgctgcgccc atctcctcca acacatctaa
    11821 gataccagtt cttgttggat tgaccccgac attttttaac aacagttggc tttccggaac
    11881 aagaagtcct gctgctagaa agaaagcagc tgatgaaata tcacctggaa cagttatctg
    11941 ttgaccagtt aatttttgcg gtccggtcac tatgatcgtt ttatcttcca ctgtgatcct
    12001 tccgccaaat tgacgaatca tttcttccgt atggttacgg gaacgttctt tttcaacgat
    12061 tacggatgtg ccttctgctt gtaatgccgc aaacagcaaa gcagatttga cttgcgcact
    12121 ggctacaggc atattgtatc ggataggtga taaagagcgg gttcctgtca cagtgattgg
    12181 tagatactgg tcattttctt ctccttgcaa gtcagcgccg atctcgcgta aaggttccat
    12241 cactcgcccc attggacgtt tagacaacga agcatccccg aacagagtag tcgtaaaagg
    12301 ctgtccagcc aaaataccca tcaataaacg tatggtcgta ccggagtttc ccatgtcaat
    12361 tgattcggtt ggttggacaa atgagtggct accgcgacca tgaatcttga tcacctcttc
    12421 ctcttcttcg atctcgacac ctaattgttg gaaggcatgt aaagtactca gacaatcttc
    12481 tgcgcgcaaa aaattttgta tagtcgtcgt tccttcagca attgctccaa acatgatgct
    12541 gcgatgggaa attgatttat ctgctggtat cctaacagtc cctcttaatc catgtatttg
    12601 ttgtaataat tgcatttatc cagcctccta tttgatccag caatgataac tggtattaga
    12661 cataatgatt ctttttgctt gtaacaaatc ctgttggttt ttaaaactga tttgcagtat
    12721 tcctaaaata tcttccctgg tctcttggat cttcaaatta atcaaagaaa ctcccgcttt
    12781 tccgatcagt ccagttactt ctgcaataac gccaggttca tctggcacat cgataaataa
    12841 atcaaaaaag gcaggaatcg cgccttgctt atggacaggc aactggtctc gactttcttt
    12901 tgcccgattg aaaaaagaaa agatttccga ttggttctca gtcatgatcc attctgctat
    12961 ttgtgtcatt tcttgttgcc aatctgccaa tcgttccaat aatatctgct tattgctcaa
    13021 caaaatatct gtccacatga caggatctga ggaagcgatc ctagtgatat ctcgaaagcc
    13081 tcctgctgcc agttgtttgg ctcgcggata ctcttgattg aattgttctg cctgattgac
    13141 taagccagct gccagaatat gtggaaaatg acttagcatt cctgttatac ggtcatgttc
    13201 tcttgctgta agagttacat attttgcttt ggttccgcaa aatagttctt gcagtttttc
    13261 aacatctttt ttcgtctttt ccaaaggtgt aaagatgtaa taggcatttt caaatagttt
    13321 agcatctgct gcctgtatac ctgatttatg agaaccagcc attggatgtc caccgacaaa
    13381 ccgaagattt ttctgctcag cggatgtcac gacttgctgc ttcgtactgc tgacatccgt
    13441 aatgatgacg tgatcagaca agttgattct ttcaaggaca tcaagataag ctaaggttgt
    13501 acggacggga acggctaata agataacgtc tgcttgttct gcacctgttt caaaatcact
    13561 tgcaacgtaa tcaacgatct gctgttctac tgcttgctca cgggtttttg actgactatc
    13621 ccaaccaact actatcagtt ctggattggt ctgacgaata caaagtgcca tagaagcacc
    13681 aatcagtcca agccccacga taaaaacatg ttgtgccatc ttatttgccc cctttttagt
    13741 aatgtttcaa gtagtcacgg tactccttca ctgcagcttt caactcaaca agcgaatcac
    13801 ttgggaattt tgacaatagc tctgtcgcta ccacagtggc tacaacattt tccgcaacga
    13861 cgctagctgc aggaactgct gtactatccg aacgttctac acttgctttg taaggttctt
    13921 ttgtatcgat atcaacactt tgaagtggct tgtacaatgt aggaatgggt ttcatgaccc
    13981 cgcgaatgac aagtggttcg ccattggtca ttccgccttc gaagccgcct agattattgg
    14041 tacgtcggaa atagcctctt tcttcttccc ataggatctc atccatcact tctgagcctg
    14101 gtaatgaacc agaagtgaat ccgaccccaa attctacgcc tttaaaggca ttgatactaa
    14161 ccacagcttg tgcgattttg gcatctagct tcgtgtccca ttgtacgtag cttcctaatc
    14221 cagctggaac gcctccaacg agcacttcta caattcctcc gatcgtatct ccatttttct
    14281 ttgtctgatc gatcaaatct tttattcttt gctcaactgc tggatcaaca actcgtactt
    14341 ctgacgcttc agaaatttct ttgatttctt gaatcgagat atttccaggt atttctgctt
    14401 ggattccacc aagacaaatc acgtgtcctg ctacgatgat gtcaagttct tccagaattt
    14461 tcttggcaat tgcgccgatt gctacacgca tggtcgtctc tcttgcagag gaccgttcaa
    14521 gcacattccg aagatctcga tgatggtatt tcataccgcc taccaaatcc gcgtgtcctg
    14581 gacgcggacg tgcaactcgc cgcagtgatt ggtctttttc aggcacttct gctacagaca
    14641 tgacagttgt ccaatttttc caatctttat tttctaccac taaagtgaca ggtgaaccaa
    14701 ttgttttgcc gtgacggatg ccagacgtga tccgaacacg atctttttcg atcttcatcc
    14761 ttcctcctct gccgtaacca gcttgtctgc gagcgagttc gtgattgatt tcttcttctg
    14821 acagaggtaa tcctgctggt agtccttcaa taatggctgt taattctggt ccatgggatt
    14881 ctcccgcagt catataacgc atcttacagt cctcctaagt aatctttgaa tgtctctgtg
    14941 ggaacaggaa cgatttttgc ttgcccaatc ttttctagca agatgatatt caattggctt
    15001 cctcttgctt tcttatcatg ggttatcgcg tggaataatt catctggatg acttagatcg
    15061 tacgtaatcg gcagatggaa tttttgaagt atcctttcta aagcagctgt cgatccagtt
    15121 ggagacaagc cgattttttc tgcatgagaa gtgatggcta ccattccaat agctactcct
    15181 tctccatgac tgactacccc atatcctgct gtattttcaa ttgcatggcc aattgtatga
    15241 ccaaaattta aaataagtcg atttccttgg tcaaagacat cttcttccac tacttttcgt
    15301 ttgatttcca acgtagcagt aatgattgga acagcatgtt ccagaagatc tttttcgtct
    15361 ttcagctcgt tcagctgttt ccaaagcgtt gcatctgcta tagctgctga tttgatgatt
    15421 tcagcgattc cttctcgtaa acgacgtacc ggcaaagttt ttagaacctc aggatcaatc
    15481 aatacaccag ctggctggct aaatgttccg acaaggtttt tcgctttgtt tgtattgact
    15541 gctgtcttcc cgccaatact agaatctact tgtgcaagta gcgtagtcgg aatttgaacg
    15601 aaatggatcc cacgcatgaa cgtcgaggcc acgaatgctg ctagatcacc caccattcct
    15661 ccaccaagtg caagtatccc atcgctgcgg gtaaaatcat tatctgctaa aaaatcgtat
    15721 aactcagctg cttgttctaa agatttactg gtctcacccg ccggtacgat tgaaaggact
    15781 ggttcaaaat tagcttgtct caattggaac atgatttttt ctgcatatag tggagcaacg
    15841 tttgtatcag tgatcacagc tactttttga ggtctccaaa tcgtttgaag ccatgatcca
    15901 gcctgttcta gtgcgccttt ttcaatatga atatcgtatt tttggttatc aacatgtaca
    15961 gtaagcattc gtttcacttc tttcttttaa aaagctggaa ttaatcgagt gtctgaatct
    16021 tttccattgc ttctttcatg atcgcaactt ctttcatcat gcgtgagtaa gtcttttcat
    16081 tcaacgattg gggaccgtct gaccaagcat ttgcaggatc aggatggatc tctacgatca
    16141 tgccatctgc tccgcttgcc acacccgcac gagccattgg tggtacaagt tcccaaattc
    16201 ctgtaccatg acttggatca acaatcacag ggaaatgagt taattttttc atcaatggga
    16261 cagcacttaa atcaaacgta ttacgtgtcg ctgtttcgta agtccgaatc ccacgttcga
    16321 taaaaatgac aggagatttg tcttcgactg caatgtattc tgcagcatta agccattcat
    16381 tgatcgtacc ggaaattcca cgtttcaatc caacaggttt acctgtttta ccgacagcag
    16441 atagcagttt aaaattttgc atattacgtg cgccaatctg taggatatcg ctgtactcag
    16501 ccaccatatc gatatgccct tcatccatca cttcagtaat cactttcatt ccaaattcat
    16561 cagccgcttg acggatgtat ttcaatcctt cttcttctaa accttggaaa gcatatggag
    16621 aagtacgtgg tttgaaggca ccacctcgca gaatttttgc gcccccggct ttggccattc
    16681 gcgcacattc tctgatttgg tctaatcctt cgatagaaca aggaccagcc atcgtcacga
    16741 aactgccgtc accaattttt actccgtcta catccacgac tgtattgcca ggatggaatt
    16801 ctcgagaagt caatttgtat gtgtttaaaa tacgcacagc attttctact ccttcatagc
    16861 gtgtaaatgc tccttcttgt aaacttcgcg tatcaccttt tagtccaatc actactcgtt
    16921 ctgttccttg atcgattgag actttgaatc cttctttttc gatatgatcg acgacttttt
    16981 ttatttgttc ttccgttgct tcttttttca taattactat cattgttttt cccgctttct
    17041 tttgtttact tatctatctt ggctgatttt ctctactttt tcaaggtggt tttacattaa
    17101 ttcctactaa atgaccgtgt cctgttgttc tcattttcgc tccttctttc ctacttatga
    17161 cccagataat caacaaaaaa agatgtccct gtaggaaatt gcctctacag tgaacatctt
    17221 caacgagcct taaaaagcgc tgcaaaaata taaaatagcc actatagatg aatttctcta
    17281 cgtggctgta agtcgtgttt aaaaatcttt agccatcata gatacaaacc tggtttgttt
    17341 gtacccacga agtcatgaat acaaacctat ctaaaatagc taaagtaata cgtattcagt
    17401 tttgtaagtt ttctagattt cataaaaaat cgctccaaac tgatgtaaga attgtaccta
    17461 gtatagacat aaaaaaatcg actgtcaatc atttttatga aacttttttt aattttctga
    17521 tagttaactt tgtttttcag aaaaataaca attggtttta ttgaagaaaa tagtcatgaa
    17581 caaaagtatc tgagaacttt tttactctct tgttcatgac atatagaata aagcagcttt
    17641 gtcttgtaaa ctccgttttt caaataataa tctaaagatt gctactgaat aataaagccg
    17701 atttcatggt cctcttcgct gctgattcgg taaggtgact caacatccgc tccccagcta
    17761 ttgatacccc cgactcctct tacttttgca aaaatcgtaa gaaccgttcg tctttctggc
    17821 ggtagttctt ctatatgtgt tgcctgttcc agttcttctg ctttgaatgg taaagcggaa
    17881 aaagcaaatg gcgtatcctg ctgttcaaat cgcaacgaaa aatcttcttg atctttatcc
    17941 gtattatcca acgtttgatt tcttgttagt tctacccatt tcgtttccat atgcatgccg
    18001 cattcttggg gaacaaggta tggggaaacg gggagtcctg ccactttata aataccttcg
    18061 acaccaccag ccatccgatc cggatatgtt tcccctgata ggccttcata ctcaaaaaat
    18121 tttgctttcg ttggcataat gaaccttaaa cccaatgcgg gtaattccgg taatcctttt
    18181 tgtccataat aatgcatatt gacaagcatt ttaccctctc cagcgatttg gtaggaaatt
    18241 gtcacttggg tttttggtac tgtgttgatt tccagtgtaa aaatgatttc aaccgtttca
    18301 gcgtattcat tacttgaata ttgattattt actggagcaa tcggcaaatc gatcttttta
    18361 ttgtccacca gtacttctat ttctttgact tttggataca atccggctcc taaccacata
    18421 ccggatttaa atccaaatcc gctacctctg tcattatcgg tcgttgctcg ccaaaaaact
    18481 ggaagtgttt cccggtatag ccattctttt ccgtttttat tgagcgattc cagtccgcct
    18541 cttgtataag aaaaaatata atgcgtattc cccgatttga cacctaatgt acagtcgccg
    18601 aagacgattg caactttttc cgtatccata tgacttttat ttactgtatt tttcataata
    18661 atatcttacc tcctgcatgg cgggtttttc ttgtccatct gcaaaaacga tcccattccc
    18721 tgagaattca tagtcacttg gtttatcgtc aaagtcaccg ccatagcgca tcacttctct
    18781 gcctgtgatt ggatcagtca cttggatcgc ctgatcttta aaatcccata tatagcctcc
    18841 ttggtacatt tcataacgat caagcaacgt catgtaagaa tccattccac ctaacgaatt
    18901 ccccatgtca tgcatatatt cacataagat gaacggtttt tccgctttat tttcaaggta
    18961 gtcaatgatc tcttctggtg gcgcatacat gcagcttttt acatctgaaa tcgaatcttc
    19021 gaatgctgga tttggaaaat tcccttcata atggacaagt cgatcggggt ctactttttt
    19081 gaagtaatcg ttcatttgtc gaatgttttc ttccgcataa gattcatttc caagagacca
    19141 aaaaagaata cttggatggt tttttaatgt ttcgaaattc gtttttgctc gatcaagaac
    19201 agccgcctgc cactgaggta ctgcacctgg tacgttgtaa gaaggctcaa atgcgcccat
    19261 tttttgccat gagccatggg attcgaggtt cgtttccgcc atcacatgga ttcctgcttg
    19321 gtcgcataaa taataccaag tgatctggtt ggggtaatga gatgtccgta cgccgttgat
    19381 ctttgcattt tttatgatag ccaaatcttt ttccatatct tctatagtga tggcgcgtcc
    19441 ttttttcgga tgccactcat gtcgatttac cccgttgata atcatccgtt ttccattgaa
    19501 gtagatgatt ttatctttga tttcgatttt acgaaaaccg aaggaatagt caatatactc
    19561 cgttactctt ttttcttcgt ctatcgtctc caattcaagc ttatataaat tgggctgaaa
    19621 atgcccccac agtgccacat tttcaaatcc atcgatgcga atcgtcattt gctcttttaa
    19681 cggatattgc tgttgataga tttccttccc atcaggatct agtatcgcta cattcagctg
    19741 actgtttttg gctacttgct caaaagacaa atctacttca agtttgccat tttgacattt
    19801 gtcatctaag cgaggtttga tccaaagatc ttctaaatgg ttttgtcctc gagcaactaa
    19861 gaagacatct ctgaagatcc caaaaaatcg gaaaaagtcc tgatcttcca agaatgcagc
    19921 tgtacttctt ttgtaaacct ccacagctag aatattttct tcttccaaat aaggtgttaa
    19981 atcaaattca gacggagtga acgaatcttc ggcatacccg ataaaatgac cattcaacca
    20041 aataaacatt gcttgttcta caccagcaaa atgaatcgag atttgttttc cctttagacc
    20101 ttcttctaat cgaaaagtcg tcaaatacga accaaccgga ttgtctttag cagctgaaaa
    20161 gatcccttcc gcctctttct cagggcttgt cgtgaaaggg ggacggcgaa atacatgtcc
    20221 ctcccacgga tacatcgtat tgatgtaatg gatcttgtca taatttgcta attcaatatg
    20281 actaggaaca gtgatttcat caaaagaaca ggcatcaaaa tcttttttat aaaatgtttt
    20341 tggtcgtacc gaaggatttg gcgaataaca aaaacgccat gatccattta aagactgttt
    20401 aagggaggat gtggtttcta cttcttccct tgttgcataa caaacatgat cgctatgtgc
    20461 atttgattgg ttcactctaa aaacttgtgg atcatctaac cattttatat ttgctttcat
    20521 acttttttcc ttctctctca taactttttg aaatgaagca gttctgtttg gaaatcacct
    20581 tgaaaatcgg ggaagtaaag tccaactgtc atcaattcgg cccccgaaaa tatccgcttc
    20641 gaggtctgtt cctgataaag tgcctcatca tctaacccca tcagatatac ttcatggaac
    20701 gctggttgag ctgaagctag tatcctccct aaaaacacaa tagcttcatc ttgtttagga
    20761 gacacaaaca tccatgcggc aatattttct tcaaaaggac tcgccaaacg ataataatgg
    20821 ccatactgga tcaccggacg aatcgtttga taggatataa tttggtcttt tacttgcttt
    20881 tcttcttcag gagacagctt tgtcaaatcc agttcataac caaaaacagc gctcatagct
    20941 gtttctcctc ttgttttcaa cgaagtagat ctgtcggtct gatgatttgg tacagctgat
    21001 acatgagctg tgatggacga aatcggataa gcaaggcttg ttccatattg gattttcata
    21061 cgctccactg catccgtgtt atcacttgtc catgactgcg gcatataata aataaacccg
    21121 gcatcaaacc gtccacctcc accagaacag ccttcccata agatttccgg ataatcagtt
    21181 gtcaaatgtt caagtagttc ataaagcccg agcatataac gatgcgctac ttctccttgt
    21241 ctctgtgggt caagggtaat cgaatagaca tctgaaagcg aacggttcat atcccatttg
    21301 atgtagtcaa gaggaatcgt atctaaaatc tttctcattt gtttctcaat cgtctgccga
    21361 acatctaacc ttgtaaaatc cagtacatgt tgtgatctgg aagcagaagg cattctacct
    21421 ggctcctgca taagaaaatc aggatgtgtt cgatacagtt cagaatcaac cgagatcatt
    21481 tcaggttcaa accataaacc aaatttcaat cctttgctat gagcataatc tgctatttca
    21541 cgtaatccat ttgtcaactt gccttcatac tcgaaccagt ctcctaatga agaattgtcc
    21601 gcatctcttt ttccaaacca gccatcatct aatacgaaca tttcgatgcc tagctccttt
    21661 gcttcatcaa tgatcgcctt gattttttca ctattgaagt caaagtaagt ggcttcccag
    21721 ttattgacca gaatcggccg ttcagcaaat tgatgacgac ctcttgcgat tctttcacgt
    21781 agcaactcat ggtggatctg actcattttg ttcaatccct gacttgagta agaaaggatc
    21841 atttcaggtg tttgaaaaga ctggcctgca ttcaattccc acgaaaaacg atggctgttg
    21901 atcccaccga cgactctcaa ttgattgatc tgatcttttt caagttcgaa agaatggttg
    21961 ccggaataaa caaattgaag tccgatcgct tctccagtat tttctgtcgt atgatgatga
    22021 actaaagcaa taaagttatt catatggtga ctgctcgttc ctcgacggct ttcaaatact
    22081 tttgttccag ataaaacaga ttgccgtgaa atctgtcttt cattgacatg tgctcctggc
    22141 aaagcaatga cttcatcaaa tcgcctagta tgggcaaagt ctagctggaa actagccgct
    22201 ttttctagtt tgattgtttc tccggtttca tttcttatct ttacagatcg tgtgatcact
    22261 gctctatcag taaatatggt atagaacaaa tcgaaataaa tcgctgtttc tctatcctta
    22321 agtgaaatca ccaacgtttg tgcctctgac ttgtccatca cataagtttg aggcaagcct
    22381 tcgatctctg gcttcccatc tgtaatataa tggctgtcat atctcagatc cagtaagtct
    22441 gatccattct tgcgctgaat gatcgatgcg ggcgttcgaa agtccatgct gccatgagaa
    22501 gagtattctt gcggtagtgt atctttcgac agagaccggt caggatttaa gggaacattt
    22561 cctgaaaatc cacgatctct tcggggataa tttttattgt ctttgtactg cttgactggt
    22621 ttgccaaaat aaatatgcgc caatacattg ctttcttcta tctttatcag ataagaaaga
    22681 aatgtatttc gtaaatggaa gactcggctc ttttcatcaa ataagatgaa atttttatct
    22741 gtttttctat gcgtaatatc catcatttca ctcccaacaa aacggaaacc gtttactttt
    22801 ttttataatt cctgtatact gtttactaaa caattttgtc aataaaaatt ttttattttt
    22861 ttgaggagtg tgtaaaaatg gttggaatta gagatgtcgc acgaaaggca aatgtctctc
    22921 ctgctacagt ttcccgtgtg ttgaataacg atcagacgat ccatgtggct cagactactc
    22981 ggcaacggat agtagaggca gctgctgcgt tggattatga catcagtact cgtaaatata
    23041 tcaaaaagag aatgccttcg attggtgtga tctctacgat cggcaaagcg agcgaagaac
    23101 aggatatcta ttacaaacat ttgcgagtag ggatggaaga agaagctcga cgtctgcatt
    23161 tagggatgaa tcgaatctat aatctatctg ataatcctaa acaatgggaa gatctagatc
    23221 agttaggtgc ggtgatcgtt gttgggactg ttacgaaaga atcgattacc gccttgttaa
    23281 aacaaaacat gcaaatagtc gtcgtggata accctgacat ccagcaagag gtcgatatgg
    23341 tgtatgcaga tcttgaacgg atgactaaaa ccgtactgga attgttctta gaaaatggtc
    23401 ataaaagaat agcctatatc gggggatata aagtcgatat ggatgaattc ggtgggaagc
    23461 agatttctat aaatgaaaag aggatgcgtg cctatcagca ttttatgatc gatcatcact
    23521 tagaaaaata tatgtcttat catctaggtg gctggacaga aaaacaaggt gaggtacttg
    23581 cgactaatct attagaaaat gaatcccctc ttcctactgc cattcttgtc gggagcgatc
    23641 caatggcaat tggtgtctat cgggcattga aacaaaaaaa tatccaaatt ggagaagaga
    23701 ttgttgtagc aagctttgat aatatcgaga caatcgcaaa cctctcccct ggcttgacca
    23761 gtgttcaaat caatgcgaaa tcaattgggc aagcagctgt tcgaatggca ttggagcgaa
    23821 ttgacggtat acgggacgaa ccaatcattt tgacttatcc tgcacgattg attgttaggg
    23881 aaagttttat cccaagaaca aaaaggtatt gaaagagagg ttgtgaaaag ccgcttcgtt
    23941 tcttcatact caaaacgaaa cggctttttc acaacctctt acttatactt attcacaaaa
    24001 tccaatgtct gctggtaaca attttgcgat tcttttagcg tgtaatcaaa ttggtattca
    24061 tgggtaatct ctttttttgt atctgtataa aacaaggatt gcacgggtac ctttaattct
    24121 gtcaaccgat tttctaatgc ttgaccttgc tcctggaatg agtatgcatt tccgtcagtt
    24181 atatacgttg gcggaaattc cttagaaaca tggtcagcca cactagcttc tttcaattgc
    24241 tcactcgttt tccaatcttt tgttccaatc aacgaccacg caacggtttt gacgaaaaac
    24301 ttcataaatc gatcatccga atgctgctgt gctgttagct tcaaatcaac aggtccgcaa
    24361 taagaaatgg ttcctttcag actatctttt ggaagtagat gtttgatttt catttgttta
    24421 gcatacgcat cattcgtttg tatagctgca tattgcaatg cgatttgtga acctgcacta
    24481 tctccaccaa aaaataattg ttccatgtct agcaatttgt cgtctttttc ttcagctaat
    24541 aaatctttga tcagttcatt cacttgaaga acttgattag ggtattctga atcaggcgct
    24601 aattcataat tcatcgcaac cactgcgatg ttggcgtctg aaactaattt tgtcgcaaat
    24661 tctttgacac ccgatttatc tccgccgacg aatcctccac catgtaccca aaatagaaca
    24721 ggaacaggcc ccttgctgtt ttttggatag tagatgtcat aagtattctc tttatacttg
    24781 gactcatacg tcaaatctgt ttttttcgcc acgttttttt ctgcttgatc gaacgttttt
    24841 ttatcagtga tttgaacagt atttgaaaac atatggttga tcaagaatgc tccaggtctg
    24901 ggggaaagct ggaaggcaag aaagattgca aacgcgatcc caagaatgat tccgaagaac
    24961 catccgataa atttaagaat tttctttttc ataaggctcc ctcagtagtt agtattaagc
    25021 attctgatta taccatagtg ttatttccta tcaagctctc ctagatgatt taatgatttt
    25081 ccccaaactt cttctgcaat gcttttgaca aaagaaattt tttccattgt tcttcaaaca
    25141 aaacattttc ttcttctgtt gaagcaaatc cacattgagg acttaagcac aaacgatcca
    25201 aaggcagata tcgtgtcgct tcgtagatcc ggtcaatcac ttgctggtgc tcttccattt
    25261 cgccattttt cgttacagtc aaaatctaag acaactaatt tatcttcaga gactttctct
    25321 aaaagaacaa aactcccagc acgttcacta tcgtattcta aataataggc acggacattt
    25381 tcttgagtaa aataatgggt ctgctacgtc gtcatccccc ctgatgccgc caaaatcgaa
    25441 taataatttc cgcgtcaaac atgtatattt agtgttgaat catttgagaa tatgaaaata
    25501 aaaatctttt tgatcaatca gttaatcaaa ttgaaataac gaaaaatctg agacagaatg
    25561 acgttcattc tgacccagat ttgattttgc acaactcaac agtccttatt ggcaaatcgc
    25621 caaaataatg tagactaact aaaa
//
LOCUS       DAZABD010000043        24271 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000043 DAZABD010000000
VERSION     DAZABD010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24271
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.043"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..572
                     /locus_tag="ACHALG_001795"
     CDS             <1..572
                     /locus_tag="ACHALG_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFN2587180.1"
                     /translation="ASRIAIMSEGRVIQFDTPENILRHPANDFVEELIGEDRLLQAKP
                     NATRVGEVMLNTAITITPEKSLQEAIKLMREKRVDTLLVTDNSNVLKGFIDVETLNKK
                     RGKVSSVGDILNKHVFYVKESAYLRDTLQRILKRGLKYVPVVDEQNKVVGILTRASLV
                     DIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            576..1217
                     /locus_tag="ACHALG_001796"
     CDS             576..1217
                     /locus_tag="ACHALG_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN2587181.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDFIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            1214..2131
                     /locus_tag="ACHALG_001797"
     CDS             1214..2131
                     /locus_tag="ACHALG_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HFN2587182.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            2128..2799
                     /locus_tag="ACHALG_001798"
     CDS             2128..2799
                     /locus_tag="ACHALG_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN2587183.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(3340..3828)
                     /locus_tag="ACHALG_001799"
     CDS             complement(3340..3828)
                     /locus_tag="ACHALG_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HFN2587184.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(4029..4295)
                     /locus_tag="ACHALG_001800"
     CDS             complement(4029..4295)
                     /locus_tag="ACHALG_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFN2587185.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(4295..4558)
                     /locus_tag="ACHALG_001801"
     CDS             complement(4295..4558)
                     /locus_tag="ACHALG_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFN2587186.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            5079..5219
                     /locus_tag="ACHALG_001802"
                     /pseudo
     CDS             5079..5219
                     /locus_tag="ACHALG_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(5361..7184)
                     /locus_tag="ACHALG_001803"
     CDS             complement(5361..7184)
                     /locus_tag="ACHALG_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFN2587187.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(7782..10487)
                     /locus_tag="ACHALG_001804"
     CDS             complement(7782..10487)
                     /locus_tag="ACHALG_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HFN2587188.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(10793..11740)
                     /locus_tag="ACHALG_001805"
     CDS             complement(10793..11740)
                     /locus_tag="ACHALG_001805"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HFN2587189.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            11930..12199
                     /gene="rpsN"
                     /locus_tag="ACHALG_001806"
     CDS             11930..12199
                     /gene="rpsN"
                     /locus_tag="ACHALG_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFN2587190.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(12375..12737)
                     /locus_tag="ACHALG_001807"
     CDS             complement(12375..12737)
                     /locus_tag="ACHALG_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFN2587191.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(12748..13869)
                     /gene="alr"
                     /locus_tag="ACHALG_001808"
     CDS             complement(12748..13869)
                     /gene="alr"
                     /locus_tag="ACHALG_001808"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFN2587192.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(13885..14235)
                     /gene="acpS"
                     /locus_tag="ACHALG_001809"
     CDS             complement(13885..14235)
                     /gene="acpS"
                     /locus_tag="ACHALG_001809"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFN2587193.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(14365..15876)
                     /locus_tag="ACHALG_001810"
     CDS             complement(14365..15876)
                     /locus_tag="ACHALG_001810"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFN2587194.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(16198..17550)
                     /gene="murF"
                     /locus_tag="ACHALG_001811"
     CDS             complement(16198..17550)
                     /gene="murF"
                     /locus_tag="ACHALG_001811"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFN2587195.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLAATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(17703..18779)
                     /locus_tag="ACHALG_001812"
     CDS             complement(17703..18779)
                     /locus_tag="ACHALG_001812"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFN2587196.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            18975..20720
                     /locus_tag="ACHALG_001813"
     CDS             18975..20720
                     /locus_tag="ACHALG_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HFN2587197.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(21000..21437)
                     /locus_tag="ACHALG_001814"
     CDS             complement(21000..21437)
                     /locus_tag="ACHALG_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFN2587198.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(21421..22794)
                     /locus_tag="ACHALG_001815"
     CDS             complement(21421..22794)
                     /locus_tag="ACHALG_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587199.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(23116..23604)
                     /locus_tag="ACHALG_001816"
     CDS             complement(23116..23604)
                     /locus_tag="ACHALG_001816"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN2587200.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(23626..24228)
                     /locus_tag="ACHALG_001817"
     CDS             complement(23626..24228)
                     /locus_tag="ACHALG_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFN2587201.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         6959 a         5163 c         4487 g         7662 t
ORIGIN      
        1 tagcaagccg tatcgccatc atgagtgaag gccgtgtgat ccaatttgat acaccagaaa
       61 atattttacg tcatcctgca aacgatttcg tagaagagtt gattggagaa gatcgcctac
      121 tacaagccaa gccaaatgcg acaagagtcg gcgaagttat gctaaataca gcaatcacga
      181 ttacaccgga aaaatcatta caagaagcaa tcaaactgat gagggaaaaa cgtgtagata
      241 cgttgttagt taccgataat tcgaatgtat taaaaggatt tatcgatgta gaaacgttga
      301 ataaaaaacg aggaaaagta tccagtgtcg gcgacatttt gaataaacat gttttttacg
      361 taaaagaatc cgcctatctt cgtgatacct tgcaacgcat tttaaaacgc gggctgaaat
      421 acgtgccggt cgttgatgaa cagaacaaag tcgtaggtat cctgactcgt gcatcattag
      481 tggatatcgt ctacgatgtg atttggggag aagatcccgc aactactgat gttgcagagt
      541 cagctactcc tgaagaatct aaggaggtct gacctatgca gcagtttttt catcagcacg
      601 gttcagaaat tctctcaaaa ggttttgaac atctttatat ttcaggactt gctttgatca
      661 ttggtatcat cattgctgta ccgcttggtg tcttactgac acgttttcca aaagctgcca
      721 cgattgtgat tggcttaacc agtgccttgc agacaatccc ttctcttgct ttattggcat
      781 tgatgatccc gctatttggc gtaggtaaat tacctgcgat tatcgcactc tttatttatt
      841 cattattgcc gattttacgt aatacgtaca ttgggatgaa aaatgtcgat tggaattatc
      901 gggatgtggc aaaaggcatg gggatgactg agttccaatc cattttttcc gttgaattgc
      961 ccattgctat gccaacgatc atggctggta ttcgtttagc tgccgtttac gtgattgcat
     1021 gggctacact tgcctcttat atcggcgctg gtggtttagg cgattttatt tttagtggac
     1081 tgaataatta ccaaccagat ctcattttgg caggtacgat ccctgtaaca attttagcac
     1141 tattggctga ttggcttcta ggtctcttgg aacaccggct gacaccgatc gcacttagag
     1201 aggagaatga agaatgaaac gtatactaaa aatgaccatg gttcttttct ctcttcttat
     1261 acttgcgagt tgttccttcc ctggtcttgc cagcaataca gatgaagaca ccatttcgat
     1321 cacaggtgga atcacttcag aagcacagat cttagcaagt ctagttgccg gaatggttga
     1381 acattataca gataaaaata ccgcaatcat caacaatctg gctactacaa ctatcaacca
     1441 tcaagcgatg atgaacggag atgcttcaat ttcagcggct cgctacacag gaacagattt
     1501 aacgactaca ttgaaccttc ctcctgaaaa agatccgaaa aaagcgtttg ctaccgtgaa
     1561 agatgaattt gagaaaagat acggtcaaac gtggttcccg tcctatggtt ttgaaaatac
     1621 gtatgtcttt cttgttcgaa aagacaccgc acaaaaatac catctatcta aagtcagcga
     1681 tctgaaaaat gtagcggatg aattagtagc tggtgtagat acttcttgga tcaatcgtaa
     1741 aggagacggt tatgacggct tccaagaaac ttatggcttc tccttcaatt cgatattacc
     1801 tatgcagatt ggacttgttt atgacgcagt cgaagcagga aaaatggata ttgttcttgg
     1861 ctactctacg gatggacgga tcgccagtta tgatctagtc atgttggaag atgatcgtca
     1921 attcttccca ccatatgatg ctgctccagt tgtagacaac aagatattag ctgatacgcc
     1981 tcatttaaaa gaagccttaa ataagttagg tcataccatc agtacggaaa aaatgcagga
     2041 attgaactac gaagcagata ataatctagt ggaaccatca gtagtagcac aacgtttctt
     2101 acaagaaaac cactattttg aagggaagtg aagcaattgg ataaaatgaa tgtctttcaa
     2161 caatttttct attatttcca ggaaaatggc tcctatatct ttgcacaatt tatccgtcac
     2221 ttcctgattt ctatctatgg tgtcctcttt gcagcagttg tcgggattcc ggttgggatc
     2281 atgatttcta ggagacgtaa attagcaaac tgggtgattc gtttggcgaa tatcatccag
     2341 acgatccctt ctctagccat gatctccatt ttaatcattg gattaggcct gggagtcaat
     2401 gtcgtgattg ttactgtatt tttgtattcc ctcttaccga tcatcaagaa tacgtatacc
     2461 gggatgatcc aagtagacaa aaacatcctt gatgtcggca aaggcatggg gatgactgct
     2521 agacaacgac tattcatggt agagttacca ctctctgttt ctgttatcat ggcaggcata
     2581 agaaacgcgt tagttgttgc catcgggata accgcgatcg gtgcattcgt cggtgctggt
     2641 ggtcttggag atatcattat ccgaggaacc aatgcaacag agggcacatc gattatttta
     2701 gcaggtgcat tgccaactgc attaatggcg atcatcacgg actggctttt aggtattttg
     2761 gaaagacggc tagatccagc cagccgtaca tcacgctgat gttattgtaa aaaacaaaag
     2821 cttctgcgac catttgttgg tcgcaaaggc ttttttagtt ctgtcatttt taaggcaata
     2881 atccactctg attcttccct ccttatgggc aaagagttgc ttttcttagc tatttatcca
     2941 ttccgtatac tccaacttca tactgccaac gggttcatat ctgcatccac cgactaagtt
     3001 tttttcttat ccgctaaggc attttgccaa tccattttcc catcactcgt catggaattc
     3061 ataccacatg atctctaaat actcgtctgc ttgttctttg aattgggcat caattcttac
     3121 atcctttggc gtggctttca tttaagcctt ttccatcagt cctcgattgt tcgatgcctt
     3181 cagctgcatc atcaaaactg gtgactgccg tatcggtaac ttcgatcatc ttttatacaa
     3241 tcgggaactt gccatcctca tccttggcta aaaacatatc tagccattcc agcttctcgc
     3301 catgggaggt ttgcggttcc taaaaccggt acacgattct tatggactgc atccgtcgca
     3361 actagacttg gcgatacgat gatccttcac cagaagagca cccccgtaaa ccaattgatc
     3421 gatatattgc caataagaaa agacattggc gtaaaagata tcgatccctc gtgatgcatt
     3481 gccgttgata ctgctattca tggccgaaag cgccacgact ttaacttctt ccgtttgagt
     3541 gacattcatg ttcggcaatg tcgctttgga tacccgctct gctaaaggaa cgacactggt
     3601 attgtatttc gtatctgtat cttttccaaa agaccatgcc aacgaatcct ctgggaacca
     3661 ataagacgac tccggttgat tgtccattgc ttttttcaac atacttttgg ctgtttctcg
     3721 ataaacgaca tttccaattg tcttcgcctc actttggcga taatcgatgg tcgttgccac
     3781 tgccaacact aacaacgatg ctccaatttt actgattttc cttgtcacac ttcttcctcc
     3841 ttgttttaca taaacccgtc aacattccat tcctaaactc aaacaatgac ccttcaaagg
     3901 tggagatata ggaacaaatt aaagaaatca tgcaataaat ccaagaaggt tggttttaga
     3961 acaaaaaaag agtctccttt ttggggagac tctttgttaa ctatctaaaa aggttggctc
     4021 ttctcacttc actcataatg tgtccaggca gaataaattt ctacaacttt attttcctca
     4081 tcaactttgt aaaccactcg atgctgtcta ttcagcctgc gtgagtatct gccttcatgt
     4141 gtaggtctca gcttttcaaa agactgcgtg ggcatatatg gatcttcctt caatgtctga
     4201 atcacttctt caaattgttt ctttaaattc gtttgcttga tttttcttaa atccactttc
     4261 gcagagtttt tgattgcgac tgtatagtta ctcatcaaag agcttcccaa tcaatatcat
     4321 cgatattcgt aaagctactg tcgtcttttt cgcggtctcg tactttatct aaagtacctg
     4381 tttgctcaag aaaaagggtt tcttgaatcg ctctccagtc ttccagccca atcaatacag
     4441 cattgtttcc actacgatca ctaataatct cgatttcagt atgattttca tttacctctt
     4501 tcaataactg ataaaaattt ttacgagcag tgctaggatt catcgttgta ttagccattg
     4561 tctctcctcc tttttctgat ataattatag cgtacttgat taagtacgtc aaaaaaatac
     4621 aataaagcac ttaaattcag caaacttttc ctctttttta catagagaaa aagacccctt
     4681 tttagggagg atctttgtcc acgctcactt cttactcctt tacgactagc ggtagccaaa
     4741 cgtaaagtaa ttggtaacaa cgtatcatct ttagcaaagg tcgaatccaa agcctgtgag
     4801 tctatgcaac aacaccaata cacgaaggtt cctcgttgca gtgtgacttg attgatatct
     4861 tgaataacct gaggatacgc acgaaagatt tgtaacggtt attcaaaact gattttggcc
     4921 gtcaagtctt ctactgcctc aaaaatgatt gcctctttcg tctcctgtca ttcatgttca
     4981 ctatcgattt gatacttgct tagaccgttg ggtttgtgaa tcaccaatta aaaatggcac
     5041 tgagttatgg taacaaaaaa tggtcacttg acgaggctga gattcctact tatgaagaca
     5101 catggcactt aagagtcgag gtaaaggaag aagctgaaaa tataggaggc tgtacattat
     5161 ttggcaaagg attcggaaat tatgatcaag acatcaaaat caaaatgttt tacagctaaa
     5221 taggaagaat aataacattt catacgctgt tcccatcttt aatggcttat agagggcacc
     5281 tttagctgag aataagacaa aacagaccct ctagcttcaa ttaagagcta gagggtctgt
     5341 ttttgatttt aaaacttggt ttatttcttc aaatggtagc tataggtgct gacaacgata
     5401 ttttcacgcc aagacaatcg caatcttaac cgcaaacttg tttggttatg gagaatattc
     5461 tgccagcgtc tgtttggtac aaattgagga atcaacacag tagttgtata gttttgtttc
     5521 gctgcatttt tacttacgat atctacataa cggataattg gattttcaat cgagcgataa
     5581 gaagagtgga cgattgaaaa acggacatct gggaaatgct ttttaaattc tgcttcgatt
     5641 tctttttctt tttcgacatc ttcgtctagc gaaacatgca tcgcaacgac ataatcaccg
     5701 atcgaacgtg cgtaattcaa cgcaccgata tttacccgtg tcacattccc gactaataca
     5761 agaacggtat tcccgtcata atcatgtagt tttgcatcat tttctaaacg aagctgttct
     5821 gctacttttt tataatgatc atggatctta taaaaaatga acataacgat tggcataatg
     5881 atgaagaaag gccaaatgtc ccctaaccga tagacaaaca aaatcgcaat gattgcatat
     5941 gagatgaacg cacccgtaat attggcaatc gatttgctta accatctttt tccttctttt
     6001 ttccatttga cgaccatccc tgtttgagat aaagcaaatg gaatgaacac gccgatagag
     6061 tataacggaa tcaagcgttc tgttgagcca tggaaaataa acagcaatac gatcgaacct
     6121 agcgctagtg tgataatccc attggaatag cctagtcgat ccccacgatc ttgatacatg
     6181 tgaggcatga atttatcttt tgccagattg tatgcaagaa ctggaaaagc agaaaaaccg
     6241 gtgttagcag caactgctaa aatcagcgct gtagcaaatt gaaggacgta atacaaaata
     6301 ccatgtccaa agacagcttt accaatttga gacaaaacag tgacttcttt ttcaggaacg
     6361 atcccatacc aatagttgat aaatgtgatc cccacgaaga agaaacctaa gatcaatgcc
     6421 atcatcgtca atgtagcagc ggcatttttt gctcttggct tcttaaagaa tggtacagca
     6481 ttactaatcg cctccacccc agttaaagag gaagaaccag aagaaaatgc tcgtaaaatc
     6541 aaagcgatag aaacacccgg cacagccgct cctggaagtg cagtagcttg caatggcaca
     6601 gcacctgtca cgatattata taaaccaaca acgatcagta acgagatcac aaggataaat
     6661 gtgtacaccg gaaataatag gaacgatgca gattccctca gcccacgaag attcagcatc
     6721 atcagtagca aaacgattgt cacagaaata gcgacttgat gaccgtagag tgctggcacc
     6781 gctgatgtga tcgcttcagc acctgctgag acagatactg ctactgtcaa catgtagtcg
     6841 atcaataaag agccaccgga aatcaagcca gcattctttc caagattctc actgctgacg
     6901 acatatgctc ccccgccatg aggataagca tggatgattt gacgatatga tagcgttaat
     6961 gaaatcaata aaataataac aaaagcggca atgggtagcg aataccagat ggctgcagca
     7021 gacaaagcga ctaaaacgac aacgatttgt tccgtaccat atgcaataga tgataacgca
     7081 tcagacgaca ataatgccaa agcagcaaat cgggttaatt tgtgctcatc attttcagca
     7141 gattttaaag gcttacctac taacaacctt tttaagtaat ccaatttcta aactccttta
     7201 cacacaattt tacttcaagt tttatagtcg aaaatttata gtaaacaaga tgttatagta
     7261 cgctcactga ccttaaaagt caataaactt taagaaaata attggaaaaa aacagaaaaa
     7321 tatcaagcgc tttttcaaac gctttaatac agcaaaaatc taacattttt ttatgggctt
     7381 tttctttgaa ttctataata aaaggataga acttgactta gaaaatcaag ttctatcctt
     7441 tttgatatct taacttttgg aagagagatg taaggctgct ttctacccat cattcttact
     7501 actcgaagca tagcgcagtt gtcacaacct cttgataaac ggtgtttaaa agcttgcatc
     7561 tgcggtaata agctcaacca aattaaaaac ataagaagct gtttttattt ggtcgttctt
     7621 attacttgat gcagagcagc ttttttacaa cctcttggta cacggtgttc aatcagctga
     7681 ccctcggtat tagctcaaaa aaacggcaaa atatgaggaa ctatttttcc atttttttct
     7741 ctaatactca ggtcaaaaca ctgatttcac aacctctttt tttaatgaaa aatatggctc
     7801 tcatgcgccg cttctttgaa tttcgtcatt ttttcttcga tgaacggata gattgccgtt
     7861 cctacgattc caaagacaac aaacaaaccg atcatgatcc agattgcttt cgtagcattt
     7921 ggccaataaa ttcctcctgc agattcccgt aacaagttta ccgcatgagt aaatggcaag
     7981 aacggattga tcatttggaa gaatttccct gagacttgga tcggatagtt cccgcctccg
     8041 ccagagatcg acagtactaa gatgataata gcgatccctt taccgacatt atcgaataat
     8101 gcagccaaga catagacgat catcatgaac gctaaggcga tcaagacagc aaatgacacg
     8161 ctgtagaccg gattttggac atctactcct agtaagaaaa tattccctaa tgtaacgatc
     8221 aagctttgcg caatcgccat tgtcaaaaat gtcagcatac gagcaacgaa ttgttctcgt
     8281 ttcgtaaaat tgtttcggtc tttctttcct aaatagtatt ctgtcgtagc aacacttgaa
     8341 agaagaacag cgccgaccca cagacatagt gcagtataaa acggggtgct agctgaaccg
     8401 ttatttgcga ttggataaag cgcattggtt tgtagctcaa caggttgtgt aaagaagtca
     8461 ctttcttttg ttgcgtctag tttcaataat ttgatgactt cgcctagatc gacttcttgt
     8521 tcgccttttc ggatcgcgtc tgctgctttt tggataccag ctttgatatt tggccagtca
     8581 ttgttgatca agtccgcagc agcgttcaca gctgtttcga cttctggcat tttttcattt
     8641 actgttgtca atgtaccggt gatttcattt tttagtgctg gccaatcatt ttggataaaa
     8701 tcagcggcca atcctaattt ttgctgaatg atcggtaatt cattgttgta taggtctgcc
     8761 ccttcattga tgccattgac gatggtatcc atatttccat tcagcattgt attggcatca
     8821 tggatttctt gtccaattgc aggcatttct ttttgatatt tttctaaaat tgccactgca
     8881 ttcgttacag ttgctttcgt tgagctgaga agcgaatcaa agtcgatctg gtccgctttg
     8941 ctcagtgcgc cttgtgcagt cccaatcgta tcgatgactt ggtctagtag agaagtgaca
     9001 gtggaactga tttgacctgt attgatacca gcaatgatgc cattgatatt attaacaacg
     9061 gcatcaattt gatctagcaa ttcatcaata gccttcgtgt cttcattttg aacagctgtg
     9121 ttcaaagcag ataggcggtt ttgcagattg cttagcaagg gttttaattg atttagctga
     9181 tcaataattg gctgtaattt cgtatttcca gcacttgttt gcaatttagt caggaactcg
     9241 accagtttat cgattgcatt ttgttgttga gatattccgt caataaagcc gttcaataca
     9301 ttgctgatgt ttttcttttc atcatcagtc agcttaccat cttcaatagc tgttcttaca
     9361 gtatctatta acgaacctgt cgttgttgcc acttgtccaa ttgacttcaa tgtaacatct
     9421 acactgtttg tgatgcttgg tatcgcatct ttcagctgag aggcacctga ttttgtttgg
     9481 tttgctagat cacttgcctg attttctaaa tttttcacat ctggcaagat atcttgtact
     9541 tgttggatga tcgtcaagcc ttgttttgct tcatcgattc cttggttcat tgtctcttgg
     9601 atcgaagcaa aatcgccgtc gatctctgct agttgcttac cggcattttg gatctctggg
     9661 attttttctt gcaaatcaag gatgattttt gcttgatctt tcaactcagg cattttgtca
     9721 ttcaatgcaa cgactttttc acccatctca tctactttag ggatgtaatc cacgaattca
     9781 tttgctttat tcagtttttc cttgatttca ggtagttgac tttgtaattc gaggacttgt
     9841 tttgtgtagc cgtcgatcgt gtccaaattt tcatctgttg ataaaatcat atctttaact
     9901 ttattgatac ttactaggtt cgtatcgatg tcgtaaccta tctcattgaa tactttcaat
     9961 aaagtcgaac tggctgtttt gataaattca tttgtgattt gcgattggat cgaacttgct
    10021 cctttatcgg tgattttcgg tgcaatcgca ttgatttttt catttaccgt atattcgatt
    10081 tttggtttct tgatatcacc actagtaaaa ctcagcagat cttctgagaa atcctttggc
    10141 aagtaaatac ctgcgtaata ctttccggaa cggactccgt cttctaattg ttcttttgaa
    10201 tcaacgaatt gccatcctaa ttgtttattt ttatgaagcg attcgatgac ttgctcccca
    10261 atggccactt ctttaccttg aaactctgct ggcttgtctg cactgtaaac agcaattggc
    10321 agctctccag tatttccata tggatcccat aatgctttga tgttaaacca cgcatatagc
    10381 gaagggatga tcatgatcgc gatgatcaga agtgttgcaa tcgggttttt gaaaatccgt
    10441 ttccagtcaa gcaaatataa tcgaagcgta tttttgattg actgcatagt tctccccctt
    10501 cttctttttt cagctgaatt actttaccat gtatttcata cttttttcaa tcggcttttt
    10561 tcaaaaaagt cgaatttttg gacatatcac ggcaattgtc tcattgtaat ctcttacata
    10621 ttgagaaata attaaggatt acagccttta ctttatcatg aaataaaagg gaaagataat
    10681 tttatgccaa agtttttatc tattatacgt atacgcaaaa aagctaaaac attttttgtc
    10741 tttgccccta caaaaatgtt tcagcttttt tcgtttttta ttcctgtatt tcttattttt
    10801 cgaatgacag gatgattttc ccagcaggag gatgtccttc aactagctga tgtgcttgga
    10861 tgacatgctt aagtgaagca gacagattca ttgaaataaa aacatgaaag gctccttttt
    10921 ggtaggcttt gattatgcca gcaaaggctt ctgcatctag atattccttt cttggaacaa
    10981 acgattcata aaagccttcc tttttttgcc gcaaatctag atctgggagg tcatttaatg
    11041 caacatacct gccgcctggt ttcatgatct ggatgccggt ttctcctttg atacttcctt
    11101 tcgtcgcatc gattacaagg tctgcctgat cagcaaattg cagacctgga tttgttttat
    11161 cataagcagc aaatgctgaa gctcctaatt tttttacctt ttcctcattt ttactggatg
    11221 cactggttaa gattcggatt cccttttcat ggagaagctg gatcagactt gacccgaccg
    11281 cacctgatgc tcctagtatc attacagtat ctgtcggctg gatttccgtc aaatggttga
    11341 ttagattata agccgtaatt cctggcgtga ccattccggc tgcttcttcc caactcattt
    11401 tatctggaat ttttgctact tttgctgatg gcaacacgac ttcttctccg taagtcccgc
    11461 cgactgcatg gacagctact cgatcaccta catgaacagt tgtgacatct gaaccaatct
    11521 ctgtcacaat accagcgcca tcattacctg gtacataagg gaatttcaat gttcgtatct
    11581 ctttcatttc tcctaaacgc aaggcaacat cataaggatt cacactgaat gcttttattt
    11641 ctacacgaac atgtccagaa ccgacttctc tgggatgagc atcgatttct tcaaaaacat
    11701 cttgtgcttc tccgtaaccg ttaattgcaa atcttcgcat ggtcaccatc tccctttttc
    11761 tttaagttta cgtttatgtc tttattttga caaaaaaaat gtcttttcgt tttggcgcat
    11821 gattcaaaac aaaatagttg tcttttttcg cgtaacgtgt taaattattt atctaaaagc
    11881 gtaaagatta cgatttacgc taataaaaaa taattggagg aatttgttga tggctaaaaa
    11941 atcaaagatt gccaaagcaa aaaaacagca agaaacgatt gaacgctatg ctgctttacg
    12001 ccaggaatta aaagcgcaaa aagattacga aggcttgcgt aaacttcctt tagatgctcg
    12061 tccagaacga ttgaaaaatc gtgatttaat cgatggacgc cctcgcggat acatgcgtaa
    12121 attcgggatg tcccgtataa aattccgtga attagcacat aaaggtttga ttccaggagt
    12181 aaaaaaagcc agctggtaat ccaagtctgg ctgtaatgta aaggaggagg gcgcatggac
    12241 cactccttgg atcatcaaaa aagatacgtt cgcttcttct agcagaaatg ctggtactag
    12301 aagcagacgt atcttttttt acgcacagct ggtccttttt tatatgtaaa ggctgcatgg
    12361 ttacttcgct atatttattt agtaacagga gtcagtgctt cgatacccac actaattttc
    12421 aatgctcggt caacttctgc cataatgggt cgatccaaat ggcagacctt ttctttcaat
    12481 cttgatttat cgatcgttct gatctgctcc aacaggataa cggagtctct ttctattcct
    12541 gttccagtag ctttgatccc aatgtgagtc ggaagcttag gtttagccat ttttgccgtt
    12601 atggccgcaa caatgatcgt cgggctaaaa tggttaccta gattattttg aataaccaaa
    12661 actgggcgtg ttccgccttg ctccgatccc acgactggag aaagatcagc aaaataaata
    12721 tctcctcttt ttaccatagg atcctcctta ttcttgatat tctcttggta tccgctgtcc
    12781 taatccgcaa gccacttcat agtggatcgt acctaattga tctgctatat cctgcatagt
    12841 gatttctttg tctgcatttt tgccgataag cgtcactttt gttccgacag gaaattcttg
    12901 aggcaatcgg atcatcagtt gatccataca tacacgccca atcgtttcac agtagttccc
    12961 ttctaccaat agagaaaaac cctgcatctt tcgtggccag ccatctgcat aaccgatcgg
    13021 tatcgttccg atccactctg cttcaggagt gatatatgtt tctccatagc cgattccttc
    13081 acctgctggg agttttttga cttgaatcaa ttcagaaacc agctcaagtg ctggctgcaa
    13141 tggataaact tccggtagtg cgtgcccaga gggattaagt ccgtacattg ctactccata
    13201 acgaatcata ttgccgattg tttcatcatg ccataaagcc gttgcactat tgctgacatg
    13261 tacatatcga ggaagttcgg gaagtttttt cagcacttct ataaagcgtt ctttttgcag
    13321 attccaatat gtatcatctg cctgatctgc tgtagaaaaa tgggtaaata aaccttccca
    13381 ttctaataca cgactttctt tgatgaatgc agctgcctct tttacttctt caggtgtaca
    13441 aaatccgata cgacccatcc cagtgtctgc ttttagatga atcgaaagag gcgtctctag
    13501 cttacttttc tctaaaacag cagctgctgc ttgcaaccat tcccgtgtac ctgcagttac
    13561 tgataaatca tgagcaacag ctacagtcag tgcgtcgtaa ggaaccatat tcaagataag
    13621 gatgggctga gtaaatccag cttctctcaa ctcgactcct tcatctaaat ttgaaacaca
    13681 aaagcccgaa gctccacctg caactgctgc ctcagctgtt tcaatagctc catgaccgta
    13741 tccgttcgct tttactactg caaacaattc ttttccttgc ggaagtcttg caacttcatt
    13801 tgctacattt tcagttatag cttttttatg tatcactgct ttcgttgggc ggtgccaagc
    13861 gacaaccatc gtttttcctt ctttctagtc ttccaaaata atttgtgcaa aagctgtttc
    13921 atttgtatga gtaatactga cccatacatt gccttcatga ggtgaacgcg tcacaacagg
    13981 ctgaccattt tcatttttta aaatctcgac atcttgaaag gtaacttttc cgatcccagt
    14041 tccccaagct ttagagaaag cctctttgca agcgtaacgc ccgcctaaaa attctacttg
    14101 gcgatgaaaa ggcaaggact ggaatagttt catttcatca gaggtcaaga ttcgtgcaat
    14161 gaattttggt ttttcttcga tcagccgagt gatcctagga agttcgactg catcgatccc
    14221 aattcctttt atcatatgct tttctctttt caataaattt agaacactct cattctacca
    14281 aattataacc attttgccac aaataagtcc acaaataaca aaaatgaaag tttctcttaa
    14341 aaaagagaaa ctttcatttt atgtttaatc gttatttgaa cggattacaa atccgcgttt
    14401 tttatcattg tgacgtttac cgccatcttt ttggctgtta ttatttttgc tataaccgcc
    14461 ttttgatttg tttccacgat aattaccgcc gtcacgacga ttacggttgt ttccgccacc
    14521 acgtttgcca tttttattgt atcctttttt ctgcattggc aatgggcgtt ctggtgtgat
    14581 cttcactggc actgcatcag ctggatcttt tgctgtcgtt tttagcaaaa gtgcaactaa
    14641 atcttgtgca gaatattctt ctaataattt atctgctgtt tgcaggtatt tatctagacc
    14701 attttctgca agttttgttt cgatttgttc aactgcagca cctaattgac ctttgaatgc
    14761 ttctttttcg gttggtggac gtaaagtcgt catccgtttt ttcgtcaagt tttcaatcac
    14821 atgcaagtag tccatttcat ttggtgtaac gaaagtcaca gacataccgc cttttccggc
    14881 acggcctgta cgtccgatac ggtgaacgta gctttctgga tcttgaggga tatcatagtt
    14941 gtacacatga gtgacacctg aaatatctaa tccacgtgca gcaacgtctg tcgctacaag
    15001 gatatctaaa tgaccgctct taaatgaacg caatacgctc attcgttttt gttgagacaa
    15061 atcgccatgg attccttctg cacggtaacc gcgtgcttct aatccgcgtg ccaattcgtc
    15121 tacacggcgt tttgtacgtc cgaagacgat tgttaattca ggtgtttgaa catcaaacaa
    15181 gcgagtcatg atatcgaatt tttcgtattc ttttgcgcgg acatagtatt gatcgatcaa
    15241 gtcagctgtc atttctttcg ctttgatctt cacatgatgt ggatttttca tgaatttaac
    15301 gccaatattc ttgatagctg gcggcatcgt tgctgagaaa agtaatgttt gtctttgatc
    15361 aggaacttgt gaaatgattt tttcgatatc ttccaagaag cccatattca acatttcatc
    15421 tgcttcatcc aaaactaatg tttgaacggt tccaagtttc aatgtgtggc ggttaatgtg
    15481 gtcaagcata cgtcctggtg tacccacaac gatatgtgga cgatctttca agccacggat
    15541 ctgacggcca atatctgctc cgccgtaaac ggcttgtaca cgtacttttt tgtcgcgccc
    15601 taaacgatat aattcttctt gtgtttggat agctaattca cgtgttggtg cgataactaa
    15661 accttgtaat tcgtgacgat ctggatcgat tttttctaac attggtaatc caaatgcagc
    15721 tgttttccct gtacctgtct gtgcttgtcc aataacgtct tttccagcta atgctagtgg
    15781 aatcgtttct gcttggatag gcgttgcttc ttcaaaacca gcgcgttcga ctgatttcaa
    15841 caattctggt gataattcta gttctttaaa tttcaaatga atcctcctat atggttgtta
    15901 actgaggatg tagctttttt gctaccccgt cgatttgttc ggtcaaagtg tattgtttct
    15961 ttttgacagt ggccacgcac gtttttatcc attcttagcc cctgtggcac caccgaaaaa
    16021 tcactttgcg aagtttaggc attagaaaag ccaaacgggt cgcgttcagc tttatcatta
    16081 ttatgacatc tggcttcaac aagttgtttc tatggcaaaa ccagtccaat ttctcttcat
    16141 ctttcggcaa taggttattc taacatagtt tttcgttttc tgcaagttta cagattttta
    16201 tttcatttgc tgaagttgtt ctaccacttc aaataagccc atgctgttgc tgccttttag
    16261 cataatacta tctgttgctg caagattttg tttgatagca tcaatcaatt tattcttttc
    16321 tttttcaaaa tagaacacag gtagttgggg gtatttttgg ttcaattctt cttttagtgc
    16381 atgcatttgt tccccataaa ggaaaatcat ggcgaatttc tcaggatcga tatgttcact
    16441 catttgcgca tgcatattta aagactcttt ccctaattcc agcatatctg ctaacacagc
    16501 gattctgcgt ccttttgttg gcagattttt aaaagtatct aataccagtc ccatggctgt
    16561 tggatttgca ttgtagacat cgctcaaaat agctgcacca tttcctgccg ttagccattc
    16621 ggttctgttt tgagtgatag aaacatgggc gagcccttgg cgtatctcag gtatggacag
    16681 tccaaagaag cgcccgatcg tatacgcaat caaagcattc gttacattat aacttccagg
    16741 cagcgggatc gtaaatgttt ctccttctac tacaaaatcg gtttgtgctt ttccttttgc
    16801 tgtcacttcc gcatgaacat cgccatttcc aataccgaat gttgtgactg tttgtactaa
    16861 tgttgccgtt aaaggtgtca gcaacggttc atctgcgggc acaacgagta atccgtctga
    16921 tacaagtccg tcagtgattt ccattttggc tttagcgatt ccttctcgcg acccaagatt
    16981 ttctatgtgt gcttcaccga tcattgtgat tgctgcagct tttggttgag ccataagtga
    17041 aagttccgtg atttcattgg cgtgatccat gcccatttcc aaaacgatct tttctgtatc
    17101 ttggggcatg tgcaaaatcg tgtaaggaag accaatatta ttgttgtaat ttccttgcgt
    17161 cttatatgtt ttgtattttt cagacaagac agacgcggtt agatctttcg tggtggtttt
    17221 accattgctt cctgtaattg caacgacttc tgcgcctact ttttccaagt aataggttgc
    17281 caattgctgc atggctttca aaggatctgc tacaggaata actggaaaat tttttggagc
    17341 ttgttcaaca ggcaagctcc aaaatgaagc agatgctccg tttgtgatcg cttgctggat
    17401 aaattcatgt ccatctcgcg taccagcaag aggaacgaat aaacttcctt cggttatctt
    17461 ccggctgtca aattcgacat tggtaatctt tgtcagtgca gagcattcgc tgcccacggc
    17521 ttgtgcaatt tcttgtattg ttagattcat tgagatctct cctaaaaaag gttgtgaaaa
    17581 aagcgtttgg cctaaacggc gggttcgcat gactctcact tccaagtaaa tttctctatt
    17641 cgtatttaga agctagaatg aacgcggtta cgcaatcact ccagcctctt ctttatttct
    17701 ctttattcat ttttttcaaa aaaagattga cgctgatggt atcgattcat tcctaactgg
    17761 atcagttctt caatcaaatc accgtatttc aagcccatat tttcccataa gagtgggtac
    17821 atactgaact ccgtaaatcc tggcatagag tttaattcat tcaggaataa ttcattttta
    17881 tttgtcaaaa agaaatcgca ccggctcaat ccgcttccac ctaacatcgt gtaagctaac
    17941 ttcgcgtact cttgcgcttt ttgataaact tcttctggca cttcggctgg aatctgcatt
    18001 tcgattttat tattgatata ttttgcttca taatcataga atgctacgtc ttttacgact
    18061 tcaccaggca aagtcgtccg aacgtcttca tttcctaata cagcaacttc gatttcgcgc
    18121 gcttcaattc cttgttcaac gattgctcga gaatcatact gataggctgt tgctaaagca
    18181 ttttgcagct cttctcggtt ttctgctttt gtaatgccga cactagaacc catattcgcc
    18241 ggtttgacaa acatcggata aagcaaagaa ccttcacatt gatcaaatac ttttttagga
    18301 ttttctttcc attgattctt aagtactggt acataaggaa cttgcggcac accagcagct
    18361 tgtaaaatat acttggtcat gattttatcc atggcacatg cactggtcaa tacgcctgcg
    18421 ccgacataag gcatattcaa tgtctctaag aagccttgga tcgttccatc ttccccgttt
    18481 ggcccatgta aaactggaaa aacgatggct ccttcttctt tgatttcgcc cggattgatc
    18541 acttttcctg taaagccttc ctctgtctgt ccacttgggt cccatgaaag atgcaagaca
    18601 tctttgctgg caggtttttc tgttagtaat ggacctttga cccattgtcc ttcttttgta
    18661 ataaatacga gttgaacttg gtaataatta taataaatgg catttaaaac tgaaaatgcg
    18721 gaaagaatgg acacttcatg ctctgcgctg cgtccgccat atagtaaagt aatcttcaaa
    18781 agattgtcct cctgtattct aaatcgttca gtgatttact ctcatatttt agcacaaaac
    18841 aaaaataaac gaataactta tttataaaaa aacgagccag cttctaaaag aacgagcgtc
    18901 atttattttt tgtcagctag attatttgtc ccgtttttta taaaaagtta tgataaagaa
    18961 aaggagggat gatgatggcg caaacaaatc cacgcagccg cttggcagaa atcattcgag
    19021 tgttcatcaa ttacaatgta gtgccaaact ttgtacagca aaaaaatccc gaacaggtaa
    19081 aaaaagcctt tgaagaacta ggccccacct tcatcaagat aggacagatg ctttctgttc
    19141 gagaagattt gctttcttct gccttcactc aaacattcaa aacattacaa gatagtgtac
    19201 ctagcgacac attttcaacg gtcaaaaaga cgatcgaaac agaactttct ctttcattat
    19261 cagatatatt cgatgatttt tcaaaatcgc cttttgcctc tgcttctatg gggcaggctc
    19321 atcgtgccaa gctgaagaac ggtgacgcgg tagtcgttaa gatccaacac cctaacatcg
    19381 ccgaagagat ccgacttgat cttcaactat ttgaacgagc gatccctttg atcaaataca
    19441 ttcctgaaac tagcgtagtt gatctaaaag gggtactcca agaagtaaaa cgttccttga
    19501 tcaatgaaat ggacttctta aaagaaagtc aaaatggcga acaattctat caaaagaaca
    19561 atggctggaa agagatccgc agccccaaga tttatgacgc tttttgttcg aaaaaagtga
    19621 tcgtgatgga agaaatgtct ggtaaaaact tgaatcattt aatgaacgcc gaaaacaaaa
    19681 ctgaaacttt tatcactgga attcaaaaca agcagctgaa acaagaagtt gcaaagcttt
    19741 tagttgagaa ctttatgaaa caggtgtttg atgatggttt tttccatgct gatcctcatc
    19801 cagggaatct gctattccat gtactaacaa aagaagaaca gacacaagct agccgaaaaa
    19861 ctgaaacagt ccatgaaaaa gagttcggtt cctttgcttt tcgagcctcc acttctgcag
    19921 aagaccctgt tgcgccatat accatcaact acatcgattt tggtatgatg gggcatcttt
    19981 ctgcaggact taggcaaaaa ctcacacaag ccgttttagc gctgtatacg aaagatgctt
    20041 atcgaatcga aaaagcggtc cttcgacttt gtcagcaaga aggctcgttt gatgaaagtc
    20101 ggttccacca agaactgaca agttttcttg aacagtatta tgattctcct attgatgaaa
    20161 tcaacttgca agaagtattc acacatgtcg tgacgatttg tcatcaaaat aatctgcagt
    20221 tcgatcgtga tatcacgctt ttgctgaaag catttgggac attagaaggg gtgatccgcg
    20281 tcctcgatcc tgaagtttcc ctcatggaag tcgcttcgcc ttttgctcaa cattattttc
    20341 tcactcatct ggatgtagaa gatacattga aacaaagcgg attagactta ttggaaggga
    20401 tgaaggctgc gcctaagatc cctcaacagc tccaccatct attagaaatg tggacttcgg
    20461 gacaaggaaa agtcaatttg gaattgaaaa agcaagacaa attattgagc aggatcgaaa
    20521 gtatgatcaa tcggcttgtt ttcggtatga ttttagctgc attgatcgta gggtcctccc
    20581 tgcttgtcca agcagcacct gtagaaaacg cagaggtcgt ttccttatta gggatattta
    20641 cgtatgctat tgccgcattc gtcattattt ttttggcaat cgatgcattg atccaaatgt
    20701 ataagaagag gaaaaagtag aaaaaggttg tgacagaaag aatcagcttc aagaaataag
    20761 aaggggtttt acataaattg tttctcaaat ttttgtgaaa attcggctta tttttcgaag
    20821 aggctacttc tgaaacaccg agaataaaaa atgtgaaaaa gctgttctgc acccagtaat
    20881 aagaacaatc aaaaagggaa catggaacac atttatcctg tttccctgtt ccctttttga
    20941 tgtattcgag cataaataaa ataccttcgt tacctactat cttctgtact tggtaaacct
    21001 tagtcaattg ccttttttaa cgcttgacga aaagacatat gatctttctc cgtaaagggt
    21061 tttggaccct ttgtccttat gccagctttt cttaactttc cactatgata acgttgttct
    21121 agaagcgtgc cgatattcgc ttcgtcaaat tcttttccag agtgatggaa cactcgcaca
    21181 cccttcggca atacaagtga agctaaacca taatcttgcg tgatcaaaaa gtcattcttc
    21241 ttaaccatac cgacgatttt ataatccgca ctatcagctc cgcggtctac gtagacgatt
    21301 tggacatgat tagcgtattc tttggtcgta taatggtcca cactggtcac gataaccacg
    21361 tcaagaccat aggattctcc taattttatg acatcttctt ttactggaga accatctcca
    21421 tcaatgaata gcctcattct ttttcgcctt cttccataaa tgataaacag ttactaatcc
    21481 gctcagtaaa agaccaacat tgatccattt tgccaagata agcagttgta cactttctat
    21541 cggcactgat tcatggttca gcgtgatcaa taatagatgg attggctctc ccatttcttt
    21601 tgctaaaaca aacaaaagcg tcatcaataa aaactggtgg atcacactcc ctttattttg
    21661 agtagtatac ttgacaacga tccggcgatc cattggaata gctgtatcag tttgataata
    21721 ttccttattt agccagctgc ctgttactac gtgccagaaa ctaagcagaa agacacctat
    21781 cgcaaaacca tttggaatca agaggaccaa taaagaagaa ctcagtccaa gcaattgcat
    21841 agccaatgga gaccatgtta aacgtttcaa aatccatggt ccgactattt ttgctgcaat
    21901 caagcctgct gcataaggca aatacataaa aatcgaaagt ttttctaatc cgtacagtat
    21961 gccaacataa aaactgctaa ataaaaaaag gaaattccct agcattccat ttagaaaagt
    22021 aaacagattt agccagcttg gcaacttcca ttccttcttc tggtgtccta aaacaaacac
    22081 gaacaagatg aacagcagca agaaaccata aatagcaaga tcaaaaagcc tttcatcgaa
    22141 taacaatcgg gcatttctca aaagaaacaa taagccaaag aatatcaaaa ataaaacaag
    22201 ctctcgcgta gctaccagat ttcttttgat gtctttgaaa tccagttcgt aacgcggata
    22261 atgactaacc gtatggtagg ccatgacgta aaaaagtgta taaatagtaa agcttgctgc
    22321 aatctgagtc ggtgcaggca actcaatcgc ttgatacagg agaaatagcc agatagctgc
    22381 aaataagtac tttttcccat ccatattgat aaaaccttgc gtcttttcgt gaaaattgac
    22441 cgtggtatta gcaggtacaa gccatgctgc actcagccct agcaaaacac ttgaaaaata
    22501 aaataaagga aaataaaata cgcctaatat tccaagcaat gaaccagcac caccaatcag
    22561 cagagcgatc atcagcaacg tgtaactatc tagaccaaat cgtagactgt tgactaaaaa
    22621 tagtccagtc atccgaaagg cataaaacag cgtaaatggc aaaatcgtca gccaattatg
    22681 cgagtctgtt agtcccaaaa ataaaagata aggaataaaa ataaccgtgt tacttaagat
    22741 aaagggcgtt gtttgtgtaa aattacgccc aaatcgtttg agtttttcga acatgacaat
    22801 ggctccttaa acagtcttat aactaatctg ctcttcgatt ttagcattaa aggggaaata
    22861 aaccaatttt acagaatata ttcttgtaaa aaaaaataca gaaagaagca taagcttctt
    22921 tctgcatttt tacggtactt gattactcaa gcagacaagc ccttctccac aaaagtcttg
    22981 cccgacaccc ctgcatccgg caggtgataa agatagaaat caattatcct gtgcctccaa
    23041 aatcaattag gagctctgta aaccagagtc cttctcgacc cgtcgcataa atttacttta
    23101 acacagttcc tatgtttatg cgagagcttt tttcatttta taatcgacca tttgaaatcc
    23161 ttgaccgata tctgaaataa gttcttctgt aatctcaaat ccttcatgct tataaacctc
    23221 gattgcttga tgattcccct gattcactcg caaaaagatc tctctttcct ttgtttgcgc
    23281 agcaagttct tcataccagc gaaaaactga agaagtcaat ccttgaccac gtactttatt
    23341 tagcaaatat aatttactta aataaatttt tccatttatc tcttcataag cagtgtaacc
    23401 gacagtcttt tcttcgcttt ttaataaaaa gtaggacaca ccttctgtca gttctttttg
    23461 aatctgtttt tttgactgat aagttttcag catgtaatcc acttgttttt gaccaatgat
    23521 cggcacaaaa gcttccggcc atacttcttg tatgactgcc atcagctcat ccagttcatt
    23581 ttctgttact tttatttttt ccatacaccc acgtcctttt ttacattata aatgaccttg
    23641 cgcttgatgg aataaaaaga gcatgatcat gctatacaca cccaccgacc atggtttcaa
    23701 gaaaatcaca atctgcataa attccctaaa agataatttc gtcaaaccag ctactagaca
    23761 gatcaagtcg tctggcgcaa agggaagcag caaggtaaca actagtactt ttttaatatc
    23821 tttcgttttt cttgtcagca attcatcaaa tttctgatac ttttttggcg ataaaataag
    23881 ctggacaaag gctacgccat agtaacggac aagcaaaaat ccgattgctt ccccaatcac
    23941 taggccaata caactatata aaagcccaaa tccattgcca aacatcagca tgcccgctac
    24001 tgaggagatc cctcccggca aaataggaat gatcacttgg atcacttgca acagaacaaa
    24061 tatcatcact gcatacttgc caaattgctg gataaacgct tgcaaagacg tgctcgatcg
    24121 aaaaatccca tgatgataag cataaagaat caacacaaag aaaaaaaaca gaccaataat
    24181 gggcatgatc tgaagacatt tttgtaaaac tgatcctttt ttagacatct atattcccca
    24241 catctttttt catttattaa aactgcctcc t
//
LOCUS       DAZABD010000044        24120 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000044 DAZABD010000000
VERSION     DAZABD010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24120
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.044"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            131..3658
                     /locus_tag="ACHALG_001818"
     CDS             131..3658
                     /locus_tag="ACHALG_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFN2587202.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3655..7377
                     /gene="addA"
                     /locus_tag="ACHALG_001819"
     CDS             3655..7377
                     /gene="addA"
                     /locus_tag="ACHALG_001819"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HFN2587203.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7446..7787
                     /locus_tag="ACHALG_001820"
     CDS             7446..7787
                     /locus_tag="ACHALG_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFN2587204.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8096..9139
                     /gene="pheS"
                     /locus_tag="ACHALG_001821"
     CDS             8096..9139
                     /gene="pheS"
                     /locus_tag="ACHALG_001821"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFN2587205.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9144..11564
                     /gene="pheT"
                     /locus_tag="ACHALG_001822"
     CDS             9144..11564
                     /gene="pheT"
                     /locus_tag="ACHALG_001822"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFN2587206.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11734..12744)
                     /gene="trpS"
                     /locus_tag="ACHALG_001823"
     CDS             complement(11734..12744)
                     /gene="trpS"
                     /locus_tag="ACHALG_001823"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFN2587207.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12998..13735
                     /locus_tag="ACHALG_001824"
     CDS             12998..13735
                     /locus_tag="ACHALG_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFN2587208.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13749..14564
                     /locus_tag="ACHALG_001825"
     CDS             13749..14564
                     /locus_tag="ACHALG_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFN2587209.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14577..15224
                     /locus_tag="ACHALG_001826"
     CDS             14577..15224
                     /locus_tag="ACHALG_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFN2587210.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15240..15896
                     /locus_tag="ACHALG_001827"
     CDS             15240..15896
                     /locus_tag="ACHALG_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFN2587211.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16058..16879
                     /gene="racE"
                     /locus_tag="ACHALG_001828"
     CDS             16058..16879
                     /gene="racE"
                     /locus_tag="ACHALG_001828"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFN2587212.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16882..18237
                     /gene="rph"
                     /locus_tag="ACHALG_001829"
     CDS             16882..18237
                     /gene="rph"
                     /locus_tag="ACHALG_001829"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFN2587213.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18238..18756
                     /locus_tag="ACHALG_001830"
     CDS             18238..18756
                     /locus_tag="ACHALG_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFN2587214.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18863..19363
                     /gene="cbpB"
                     /locus_tag="ACHALG_001831"
     CDS             18863..19363
                     /gene="cbpB"
                     /locus_tag="ACHALG_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0180001 - cyclic-di-AMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP-binding protein CbpB"
                     /protein_id="HFN2587215.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19643..20563)
                     /locus_tag="ACHALG_001832"
     CDS             complement(19643..20563)
                     /locus_tag="ACHALG_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFN2587216.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20689..21741
                     /locus_tag="ACHALG_001833"
     CDS             20689..21741
                     /locus_tag="ACHALG_001833"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HFN2587217.1"
                     /translation="MIKCGENNEGGKYMQIDWNNLGFSYIKTPWRFIAKWKDGQWEEG
                     ELTEDNYLTIHEGSPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLM
                     PMVPEKMFVDAVKQVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVF
                     CTPVGAYFKGGLKPTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCV
                     YLDPATHTKIEEVGAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIE
                     GDIFIDDLDQFAEAGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGI
                     QFGDIEAPEGWIEVVE"
     gene            complement(21851..22606)
                     /gene="fabI"
                     /locus_tag="ACHALG_001834"
     CDS             complement(21851..22606)
                     /gene="fabI"
                     /locus_tag="ACHALG_001834"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFN2587218.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            22826..23266
                     /gene="fabZ"
                     /locus_tag="ACHALG_001835"
     CDS             22826..23266
                     /gene="fabZ"
                     /locus_tag="ACHALG_001835"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFN2587219.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(23381..>24120)
                     /locus_tag="ACHALG_001836"
     CDS             complement(23381..>24120)
                     /locus_tag="ACHALG_001836"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFN2587220.1"
                     /translation="SISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAESTTASSVD
                     LNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAKDGYYNGLT
                     FHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARSQDPNSNGS
                     QFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGMDVVDAIAN
                     IETDSSDKPKEEVKIDKINILQEAKQK"
BASE COUNT         7980 a         4213 c         5105 g         6822 t
ORIGIN      
        1 tttgcgtata cttaaaaaag tagaaatatc agaaccttaa aaggatataa tttgaagaat
       61 atagaacaaa tgttcagtat tttttgatat aataaaggag caccgtatca tgtgttgaag
      121 gaggaaaagc atgtctttac aatttattct tggaaacggg aaaaaagatc accgtcaagt
      181 cttgatcgac gaagcatccg cttggctcga ccaggatgca agtaaccaag tatttttcct
      241 tgttcccaac tacagcaaat tcgaacagga acaagaaatt ttagcggaga tgcgaagaag
      301 aagcggaaaa aaaactttca gcacaacaaa cgttcaagta tttagttttt atcgtctggc
      361 ttggtatttg ctgcaacaga caactcttct tacaggaaat gaattatcag aatcaggatc
      421 tgcaatgatc ttgcgacaga ttttagaaaa aaatgaggaa gaattaacaa tctttcgcgg
      481 agaaatcaat aaaagagggt ttattcgtca attacaagaa ctctacagtg aattgcgtac
      541 aggaaatatt caaccagaag acctgatgct tttatttggt gattcaccta atgcagaaga
      601 ccagcaactt aaaatgaaag acttgaagct gattttttca gcttatgaca gcgaattgac
      661 tcaacgtgca ctaacaaatg aagatgcact atctatacta gcaaattata tgcagacaca
      721 agatttcaac aacgtcaaat ttattgtcag tggtttttct cgaatcaatg gtcaagagta
      781 tcaattactg caggcaatga tggaaaaggg gcaattggtt gtcgatttat tgctggatcg
      841 tccttataca gcggatcttc cagaagtact tacgctcttt catgagactg gaaagcttta
      901 tttccgtctt tttcaaagtg caagagaacg gaaagtccct atattagtgg accgatttgc
      961 accagagcag actcgtccga cagagcttca actgctgcaa acaatgtggg aagagacatc
     1021 tagccaaaag aaagcggcag tacacaaaat agggggacaa gagcatctcc atttgtggga
     1081 agctgaaaat ccaactgaag aagtccgaca attagctgtt gaaatcagac gactagtcag
     1141 tgaagaaaac taccgttatc gtgatatcca agtgctgaca aaaaatatgt ctttatatgg
     1201 aaatatcctt cggccgatct ttcaagaaat ggcaattcct ttttatatcg atgaagaaca
     1261 aatgatggaa aatcatccgc tgatcgaatg gatcaacagt ctgttcgctc ttgaccgcta
     1321 ttcttatcgg ctaagtgata ttatgcgttt tttcaaaact gaattatttc tgtcgacgga
     1381 agcagaaacc gaacttgatg aatggaaaca agagcgagat gaattcagga aaaaactgga
     1441 catcactgaa aatgtagcac ttgcttacaa ttatcaagga acttattgga ctcgtgaaaa
     1501 agactggcag tttgtggcgt atgattttga agcagaacag attgaagacg tacagtattt
     1561 ggaagaacag tcaaatacga ttagacgatt atttcagaaa gctgtaccag tatttttgaa
     1621 aaaaataaag aaaagcaaaa caggacaaga ggccgcaaat gttttttatc aatttttgat
     1681 ccaaagtggc gtacagcaac aattattgtt ttggcgtaat caggaagtag acagaggaaa
     1741 cttagaagcg gcaagaaacc atgagcagac atggggagcg ctgattgatc tgttggatga
     1801 atatgtagta atttatggtg aatcggcttt tgactggtca ctgttccaag aaatcattac
     1861 aagtggtcta gaaaatctaa gttatggtaa aatccctacc gcaatcgacc aagtgcgaat
     1921 caatcgtttg gaattagttc gaccaaatca agctgccgtc acttttgcat tagggttgaa
     1981 tgatcaagtg tttccagacc gaaaagagac aaaaggattg ttgtccagtg aagaacgaga
     2041 agtattaaat caggccttgc cagatggaca gtttttattc gacccatcaa aggaaagtat
     2101 ctcttttgag cctttccaag cctatcttgt ttttctttcc ggaacagatc gattatatct
     2161 tagttatgca caaagctatg acacagatgg ttcgctgaag atgtctcctt atttgcgtag
     2221 aatcacagaa tatttatctg ttcccgtcga gagaaaagag catttgacga tcgaaagcga
     2281 ccctaattgt tatgtaggga cttacagaag cggaatcaat acagtcaata ggatttatcg
     2341 attagcttta gatgaaaaaa aacctgtacc aaagcactgg caaaagctca aagaattagt
     2401 actgaattcg tcatggcgaa attttgcttt acagattttt gaaagtcagg aacatttaaa
     2461 tgttcctgtt tcgatgccag aaaaaatggc ggaagagctt tatggaaaag agatttatac
     2521 ttcaatttca cgtatggaga atttttataa ttgtgaatac aaatacttca tgcaatttgg
     2581 tttgaaattg aaagaacgaa ctgtttatgg tctaactcca gcagcaactg gtgattttta
     2641 tcatgaatca ttggatcgtt tcttcaagct actgttttca aaccaattat ccttagtgaa
     2701 tatgtccgat gcgcagcgta gagactttac tgaaaaagtg ctgcaggaag tattcggaga
     2761 acttcggttt gagatattgg atagttctgc aagaatgaac tatatccgct accagttagg
     2821 acagacgatt caaaaagtga catgggcttt gcaaaaacag agcaaaaata caggcatgca
     2881 tcctcttcag acagagattc tatttggtca gattgctgga gcaaaaggta tcacaggtct
     2941 ggaattaccg ttgagtaatg gtggaaaact ccatgttcga gggaaaatcg atcgaatcga
     3001 tgtagcttct gaacaagaag atacttggct aagcgtcgtt gattacaaat ctagcggacg
     3061 ttcattcgat gtcacagaag cttattatgg catggctatg caattattga cgtatctgga
     3121 cgtagcattg atggatgcgg tacgtttgac tggaaaggcg gctgttaaac cagcaggatc
     3181 atattattta catgttcata atcctgtatt gacggagaca gatgatgtag agaagaatac
     3241 attgaaaaaa tttagttatg atggcgtttt tgtcgatgat cccgctcttt tagaagtact
     3301 agatcattca ctggaagcaa aagaacaatc actgatctat ccagtgaaaa aaaatgcgaa
     3361 agatgaatac caaaaaggta gtttcagtaa agacaaattt ttcacggaag aagaactgca
     3421 actattcatg gcgcataatc gggaaaatat gcggttagct ggagatgcga ttacttccgg
     3481 agaaatcaaa ctaaatccag cttataaaga taaacagcgg attgcctgcg aattttgtcc
     3541 attcagaagc atctgtacgt ttgatgtgat gctgaaagaa aataattacc accgtctgga
     3601 gaaaatagat aaggaagaag cactgcgtag aatattgaaa aggagtgaag aagagtgaat
     3661 caaattcctt taaaaccgca aaatgaacga ttcacagatg atcagtggca agcgattttt
     3721 gatcaaggag ataatctatt agtctctgcg tctgccggat caggtaaaac tacagtgctt
     3781 gttcgaagag tgattgaaaa actaaaaatg ggttttgata tcgatgaatt attgattgtg
     3841 acatttactg aagctgccgc aagagaaatg aaagaaagaa ttcaagaggc attacaagaa
     3901 tccgtcaata gcgaaagtga ccctgtacgt agacagcatt ttactaagca gttagtttta
     3961 ttgcccacag cgaatatctc aaccttacat gctttttgcc tgacagttat tcgacgttat
     4021 tattacttaa ttgatattga tccagtcttt cgcatgttaa cagatgaaac agaaacgatc
     4081 ttgatgaagg aggatgtgtg ggacgagctg agagaagcac tttatgcaga aaatgatgaa
     4141 cgatttttcc aattaacaat gaatttttct aatgatcgtt cagatgacgg cttgacgaat
     4201 ctggtgtttt ctctttatga gtttgcccgt gccaaccctg acccccaaaa atggctagaa
     4261 cagttaagcg ataattatcg tttgcctgag gggctagcga aatctcgatt atatcaaaaa
     4321 caaatccgtc ctttagtttt ggcagatata taccaatgtg tacagttata tgaacagatg
     4381 actcagttag cacaaggtga aggattagaa aaaatgcatg agcaagtagc tggagaacaa
     4441 cagcagatca agaatatcta tgaagcgttc tcgcaagatc ggttagaaga agcttatgct
     4501 ggtttggaac aactaacttt ttcaactttt aagagcagtc ggaaagcaga attgaaagaa
     4561 atttccaatg aagtcaaggg catgagggat aaagcaaaaa aactgattca gcagataagt
     4621 aaaagttatt ttcctgtttc cccaagccaa atggaagaat taacggacaa agcactaccg
     4681 ttagtcgaag aaatgacaaa agtcacgcaa agcttcatgg acggtttcag tatgcgtaaa
     4741 cgggaaaaag gtgtgctgga tttcaatgat ctggaacatt tagcgttgca gattctcaca
     4801 gaaaaaacga aagatgtatg gctgccaagt gaagcttcaa aacattatcg aaaaaaattc
     4861 aaagaagtaa tggtagatga atatcaagac gtgaaccaat tacaagaagc cattttatac
     4921 tggctaagag aacccgatga tacaaaagga aatatgttca tggttgggga tgtcaaacaa
     4981 tccatctatt ctttccgttt agcagatcct agtttattta tcgggaaata cgagaatttc
     5041 tcaaagaaag aaggcggacg tcgaatcgtt ttggctgaga acttccgctc tagaaaagaa
     5101 gtcctttctt ttacgaatct tatttttgaa caactaatgg atccagcagt gggtcaaatc
     5161 aattatgacg aagcagccaa actgatccaa ggattttcag actttccaga gaatgaacag
     5221 tttgagccgg aaatcatgat ctatgaaaaa gaacaggaag aatcagagat tgagattccg
     5281 acggatgata tactggaaga caaaacagaa ggcgaacttt ttatgacagg actaaaaatt
     5341 cgtcaattaa ttgattcctc ctttatgatt tatgataaaa aatcaaaaaa aagtcgtccg
     5401 atagagtaca aagacattgt tctcttgaca cctacaaaga agaacaactt aacgatttta
     5461 gagattttca aaacattgga tattccatta gagatgaatg atgctcaaaa ctattttcaa
     5521 gcgacagaaa ttcgcacgat gatctcgctg ctacaattaa tcgataatcc ctatcaagat
     5581 attccgcttg cggctgtatt gcgctcaccg atcgtgggtc tgattgaacc agaattagcc
     5641 agcattcgat tagctaaccg tgcacatact tactatgatg ctgtgcttgc ttatcaagca
     5701 tcaaacgaag atgaattagc agctaagctg gaacattttg gcaagcaact agagcattgg
     5761 cgagaacttg ctcgccgttc ttcgatcact gacttgttat gggatatcta ttatgagacg
     5821 ggatatctag agtatgtggt cggtctgcca gcaggggcac agagacaagc caatctttat
     5881 gcattggttg atcgtgccaa agcttacgag caaagttctt tcagaggatt ataccagttc
     5941 gtgcgtttca ttgaaaaaat gcaagaaaaa gataaagact tagcagaacc agtcatttca
     6001 atagaagata atgctgttcg cgttatgacg atccatgcaa gtaaaggctt ggaattccca
     6061 gttgtatttt tgttagatat gacaaaggag ttcaacttac aagacttgcg caatcggtat
     6121 gcttttgaag aaaaacttgg agcaggaatt cgctatatgg atcctgaaac acgagtgtta
     6181 tatgatacgt taccgtttca agccatcaaa ttggcaaagc aaaacaaatt gttgtcggaa
     6241 gaaatgcgta aattgtatgt aggattgact cgggcagaac aaaagctttt cattgtcggt
     6301 tcctataaaa ataaagagca aatgatccaa acgtggtctg aagcagctga tcatgaggag
     6361 ttagtttttg atcctgcgct acgtttgaag ggcagaagca gcttgatgaa ttggattggt
     6421 tacggattga tccgtcatcc tgaaatgcag aaatacttag aggaagaaat ttcgacttct
     6481 cttcttcaac attcaaatgc acagttttct atttcatgga tgaatcagca atcgatcatt
     6541 gaacaaagac agttgcttgc tgaaaaagaa ctggttaact tagatcagca gatgaaagaa
     6601 gatgaaactt tattagcaga ttcgttacaa aaacgtctgg catatgaata tccttatcaa
     6661 gcttcttcac aaaccacaag ttatcaatct gtatctgaaa tcaaacgatt attcgaagat
     6721 ccagatgata cacaagaatc acgtttgact ctggaaagca gtcagaacaa agcagcatcc
     6781 cgtcaatttc gttatacgca agaacaacta gcagaaccaa aatttttgca aaaagatcgg
     6841 caagtgtcag cagctgctgt tggaactgct acgcatgctt tgttacaatt actgccatta
     6901 gagatgccaa ctacagagag tatccatcaa aaattacagg aattggtaag gaaacggtta
     6961 gtagacgaaa aagtcgcgaa aaaagtagat gtttcttcta ttatatggtt ctttcaaaca
     7021 gaacttggtc aacagctgat tgccaacaaa gaaaacgtaa aacgagaaca acctttttcc
     7081 atgctgcttc ctgctgacga agtatttcaa gactatccaa atcaagaaga tgaactatta
     7141 atccacggaa tcgtcgatgg atacctagaa gaaaaagatc atctcaatat ctatgatttt
     7201 aaaacagatt ttattttaca tccggatgat ccagcagaaa tagatgcaat cgtccaaaaa
     7261 tatcaaggac agctaagact ttaccaacaa gcaatgtcag aagctttgaa taagccagta
     7321 gaaaatgttt tcctaatttt gcttcgagtg aaacaaatta taaacattaa caaataatta
     7381 taattgagca aacttgactt atgttttgta aggattatgc tatactctgg gaagaggtga
     7441 gtatgatgga gctaacacag acagaattcg ttttatgccc taaatttgaa aaaacattct
     7501 ctattttagg aaaaaaatgg aacggattga ttatcgatgt actactcgaa aacggtccgc
     7561 aacgctttgt tgagcttgcg gcaaaaatcc cgatggtcag tgatcgagta ttagtcgaac
     7621 gcttgaagga attagaaaaa gaaggaatag tcactcgaaa ctttgcctgt agcgaaaaga
     7681 gccgttcaga atatctcttg actgaaaaag gagcagactt gcaaaaagcg atgcgtcaaa
     7741 tacaagtttg gagcgaaaaa tggatcacag aggaagaatg cggttgattt tgtaaaaaaa
     7801 atcttgtaaa ctatagtcca tacgaataag taaagaacga tgacgaaaaa gagtagtttg
     7861 gctaaaccag ttcagggaga tctgtcgcag actgaaagca gatcctgtgt ttgaaaaatg
     7921 aagttcactt tccgagttcg tctgataaaa tcagaccggc aattgtcgtt aacgaagtaa
     7981 gagtgcggtg taagtcattt atccgcaaat taggatggta acgcgaagac ctcgttcctt
     8041 aacaggaatg aggtcttttt gttttgtatt tggaatttaa aggaggaaca agcacatgtc
     8101 tctacaagca caattagaga gcttgaaaga agcaacgctc gtcaaaatca cagcggcaca
     8161 agatttaaat gaattaaatc aaatacgtgt tgaaacgtta ggaaaaaaag gaccgatcac
     8221 agaagtatta cgtgggatga aagatctaag tccagaagag cgaccaaaag tcggaagttt
     8281 cgcaaatgag atccgagatc ttttgacaga agagatcgag cgaaaaaaac aagtattaga
     8341 agaagaagcg ttaaatgcag ctttggcaaa agaaacaata gacgtgacac tacctggtag
     8401 aaaagtaaaa catggtacac gtcatatcgt gacacaaatc atggaagaaa ttgaagatat
     8461 ttttgtgggt atgggctatc agatcgtaga aggatatgaa gtagaatctg atcattacaa
     8521 tttcgaacgg atgaatctgc caaaagatca tccagcaagg gacatgcaag atactttcta
     8581 tatttcagac gagatattga ttcggacaca tacttcacca gtccaagcac ggacaatgga
     8641 aaaacatgat ttctctaagg gtgctctacg aatgatctcg ccagggaaag tcttccgcag
     8701 agatacagat gatgcgaccc acagtcatca gttccatcaa attgaagggc tagttgttga
     8761 taaaaacatc acaatgggcg accttaaagg aacattagaa gtcgtaatga aaaaaatgtt
     8821 tggggaagat cgtgaaattc gtttgcgtcc aagttatttc ccatttacgg aaccatcagt
     8881 agaagtagat gtcagctgtt tcaaatgtgg tggtgccggt tgtaacgtat gtaaatacac
     8941 tggatggatc gaaattttag gagctggcat ggtacacccg aatgtgttga agatgtcagg
     9001 aatcgatcca gaagaatatt caggctttgc tttcggcctt ggaccagacc gcgtagctat
     9061 gttgcggtat ggagtgaatg atatccgtaa tttctatcaa aatgacttac gtttcttaag
     9121 tcagttcaag ggggagtaag aaaatgttag tttcatataa atggctcaat cgttatgtag
     9181 atttatcgaa tgtaacacca aaagaattag cggataagat gtctgtgaca ggaatcgaag
     9241 tagagggagt aacggttcca gaagaaggct tgaaaaaaat cgttgtcggt gaagtcaagg
     9301 aatgcgtacc acatccagat tctgatcacc tttctatttg tcaagtcgat attggcgaag
     9361 acgaactgtc tcaaatcgtt tgtggagcgc ctaatgtgaa ggttggaatc aaagtgattg
     9421 ttgcacttcc taactcacgt atcgctggta atgtaaaaat caaaaaagga aaaatgcgtg
     9481 gccaagtatc aaatggcatg atttgctcat tacaagaaat tggctattca gatagtgtta
     9541 taccgaaaga atacgctgaa ggtatctatt atatgccaca agaagctgta aatggcgatc
     9601 cagtttttcc ttatttggat atggatgatt cgattattga attatctatc acaccgaatc
     9661 gtgcggatgc attgtcaatg cgtggcgtag cttatgaagt tggggcaatt tatcgtcaga
     9721 ctcctacatt cgatgacgaa aagctggtag aatcctcacg tcctgctagc gataaaattc
     9781 aagtggaagt tgaagataaa gaagcagttc ctgcttatca aatcagaatc attgaaggag
     9841 taagaatcca gccaagtcct caatggctgc aaaatctttt gatgaacgaa ggcattcgtc
     9901 caatcaacaa tgtagtagat gtgacgaact atattttgct tttattcgga caaccactac
     9961 atgcatttga ctacgacaaa ttgaaatcaa aagagatctt tgtccgccgt gcaaaagaaa
    10021 atgaaacatt agtaacacta gatggagaat ctcgggaatt gacaactgac aatctcgtca
    10081 ttactaacgg caaagaacca gttgctttag ctggtgtcat gggagggtta gactcagaga
    10141 tcacgaatga aacaacaaca gttgcactag aatctgcttt atttgatcca atttcgattc
    10201 gtcgtacagc aaaacaattt aatttgcgta gtgaatcctc tgcacgtttt gaaaaaggaa
    10261 tcaaccatgc gactgttgga gaagccagtg aggttgctgc agcaatgatc gtttctcttg
    10321 cgggtgggga agttctacaa ggcgcggtca aaggctcaga gatcaaagca gaagacgtat
    10381 cagtgaagat ttcacttgaa cgcatcaatc aatatttagg aacatctctt gtcgttaaag
    10441 aagtaaatga gatttttgaa gcacttggat ttagctatga agaaaaggag tatgtttatt
    10501 ccgtagtagt accaccaaga cgctgggata tccaaatcga agcagatatt atcgaagaag
    10561 ttgctcgtat ttatggatac gaccagttgc catctacact tccaagtgga gaaacggttg
    10621 ctggaagctt gtcaaaagaa caagaaacta ctcgtaaaat tcgtacaatc tttgaaggaa
    10681 gcggattgag cgaagcaatc agttatgcat tgacaacaga agaaaaatca agacagtttg
    10741 ttataaatga aagtaatatt acacgtttgg actggccaat gagcgaagaa cgttctgtat
    10801 tacgaatgaa tttgatcagc ggactattag atgatatcag ttataacact gcccgaaaaa
    10861 atacagagat tgctttatat gaaattggtc gggtgttctt ccaagaaaat gatccacgta
    10921 cacatctgcc gcaagaagta aaacatgcag caatcgctat ttctggtatt tgggaagaaa
    10981 aagattggca gacgaaagct caaaatgtag actttttcac catcaagggt cttttagaaa
    11041 acttatttga acaactaggc atcactgaag atgtccatta tcaaacagtg acagaaatga
    11101 aggaaatgca tcctggaaga actgctgcga tttaccttgg cgaaaaattt attggttttg
    11161 tcggtcaagt gcatccaacg attacaaaaa gttatgatat tccagaaaca tatgttgctg
    11221 agtttgattt atctgctata gtggaagctg ctcaaaaagg gatcgttttc gaagcagtga
    11281 caaaattccc agcagtcagc cgggacatcg ctttgcttgt aaaagaaact gtgacaaatc
    11341 aggaattaac agaagttatc cgttctgctg ccggaagatt tttaacagat atccaattat
    11401 ttgatgtcta tcaaggagaa aacattgaaa aaggacataa atccatggcg tatagcctga
    11461 cattcgtcaa cccagaagca acattgacag atgaagaaat caataaagca atggaaaaag
    11521 tcgaaaaagc gttggtagaa aacctagagg caagtattcg ctaagaagga aaatatagta
    11581 agtaacgatc aagtagcttt tgaagaccat acatttgaaa aaaagatgtg acacaacttc
    11641 gtcacatctc tttttttgat tttaaaaaag gaatagcaca gagacgagat aaaaaaacag
    11701 gcctttacag cctgcttttt tagagtaaat gaattaatgg aagtaattga ttcccattgc
    11761 tgattttact tcagaaagtg tttgtgcagc tactttagca gcttcttcac tacctttacg
    11821 taagatttcc ataacagctg aaggatcttt ttccaattct tctcggcgcg cacggatcgg
    11881 tgcaaattca gcttccaata catcaatgag gtatcgtttg atctttacat caccaagtcc
    11941 gccgcggcgg taatgagctt tcatttcttc aattgcttct ttatctgttc cgaatacatc
    12001 caagtaggtg aagaccatat tcccttctac ttgtccagga tcttgtacat ggatgtggtt
    12061 tggatcagta tacatactca tgactttttt ctgtaagaca tcggcaggat cggagatata
    12121 aataccgttt cctaacgatt tactcatttt gccatttcca tcgattccag gtaatctacc
    12181 cattcctttt ggtgggaata cgccttttgg ttccaccaag acttcaccat atgtatgatt
    12241 gaagctttgt acgatttcct gtgtctgctc tagcattggt ttttgatcct caccaacagg
    12301 tacaagattc gctttgaatg cagtgatatc tgctgcttga gaaacaggat agataaagaa
    12361 tcctgtaggc acgctttcac cgaatttttt ttgttcgatt tctgttttga cagtcggatt
    12421 gcgccgaacg cgtcctacgc tgactaagtt caaataatac atggttaatt cagctaattc
    12481 cggaatttgt gattggataa ataaagtaga ttttgcagga tctaatccca cggctaaata
    12541 gtctaaagca acttgcaaga catttgatga aaccttctca ggatttttag cattatctgt
    12601 cagtgcttgc atgtcagcaa tcatgacaaa cagttggttg tcttcatctg cctgcatagc
    12661 cactcgggtt tttagagaac cgacgtaatg ccctaaatgc agcttcccag ttggtcgatc
    12721 tcccgttaaa ataatatttt tcaaaaaaga cacctcttta cgatttgtaa gtctgagcac
    12781 ttaatatctt accgtttttc ctgaaaagaa acaacagttc ctaaggaaaa aacaaggaaa
    12841 gaggataaaa taactaatag attagaaatg gaagaaatat ttatgggaga ataaacgttc
    12901 taagaaactt tacaaattac taaactatca gatataatgt attttattat acatctttaa
    12961 aagaaatctt actaaataat aatgggagtg taattctatg gcaatgatta aatttgatca
    13021 cgtggataaa tattatggca agttccacgc attaaaaaat atcaatctcg aattcgagaa
    13081 aggagaagtt gtcgttgtca ttggtccttc tggttcagga aagagcacga tgcttcgctg
    13141 tatcaatgga ttagagacga tctcttcagg taagctgctg attaatgata ctgacttgca
    13201 tgataaaaag acaaaactga cagaagtccg aaaaaacatc ggcatggttt tccaacattt
    13261 caatttgtac ccaaataaaa ctgttctgga aaacatcaaa ttagcaccaa tcaaagtgtt
    13321 gaagcaagac caggcaactg cagtcaaaaa cgcggagaaa tttttgaaaa ctgtcaatat
    13381 gttagataag aaagattctt atccttccat gttatccggg ggacaacagc aacgggtggc
    13441 aatcgccaga ggtttagcga tgaatcctga gatgctgctg tttgatgaac ctacttctgc
    13501 tttggatccg gaaatgattg gtgatgtatt agacgttatg aaaaaattgg ctcatgacgg
    13561 tatgtcaatg attgtcgtga cacatgaaat gggctttgcc aaagaagtgg cggatcgtgt
    13621 tatcttcatg gctgatggag aagtattgga agacagcaga aatgtgagag actttttcga
    13681 aaatccaaat gaagtccgag cacagcaatt tatcagtaaa gttatcaatc attgatagga
    13741 ggaaaaagat gcgcaggtta aaaaaaatac gcctgatttt ccttttaggc attgcttttc
    13801 ttgcactagc cggatgtaaa ggaaatgggt tggcaaatga agatatttta gagcgaagca
    13861 aatctaccaa tgaaatcatc tggggtgtca agtatgatac tcgattattt ggaatgatgg
    13921 atattgagag cagaactgta caaggatttg atgtagatat tgcaaaagca atcactaaaa
    13981 agatccttgg tgacaatgga aaaacagaat ttgttgaagt cacttctaaa actcggattc
    14041 cattattgaa aaacggaaat atcgatgcaa ttattgcaac gatgacgatc acagatgaac
    14101 gaaaaaaaca ggttgacttt tcagatgtct atttcgatgc tggccaagct ttgttggtaa
    14161 agaaaggaag ccaaatcaaa agtgtagatg atttgaatgc ttctacaacg gttctagcag
    14221 tcaaaggttc tacatcggct gcgaatatcc gacagcatgc accagatgca aaaattttag
    14281 aattagaaaa ctatgcagaa gcatttactg ctttacaatc tggtcaggga gacgcgatga
    14341 ctacggataa tgcgattttg ttagggattg cagatgaaaa tccagagtat gagttagttg
    14401 gtggaacgtt tactaatgag ccttacggga ttgcgatcaa caaagggcaa gaaaacttct
    14461 tgaaagctgt taaccaagcg ctagaggaaa tgcatgcaga tggcacatac gataaaatct
    14521 atcaaaaatg gtttccaaat gaaactgaag gaaaagtaga gtaaggggga gaggcaatga
    14581 gcgaattgat acaaacttat ggaccaatct ttgtcgacgg gttcaagatc acattgtttt
    14641 cgagtgtctt agcacttgtg tttagtttga ttatcggaac gttaatggca atcttccaat
    14701 tatcaaagaa taaagtagtg agcgccttag caaaggcata tgtagaattt tttagaaaca
    14761 ttccattatt aatcatcgtg atgttttttt atgttgtcgt accgatttat tggtttaatc
    14821 tagatggatt ccaagcaggt acgattggat taacgatcta tacatctgct tttattgctg
    14881 aaacagtacg ttcagggatt caaaccgtac caaaaggtca aacagaagca ggactatctt
    14941 ctggttttac ctattcagaa atcatgcgtt atattgtttt accgcaagca ttcaaaatcg
    15001 ttatcccgcc attaggcaac caatttatca acctcgtaaa aaactcatct attttagcaa
    15061 tcgtagctgg tttagatttg atgtatcaag gagacttgat cgctagtgaa acattcaata
    15121 ccttcgatac gtatatcatc gttgggttat tttacttagt tatcacgcta ccgttatctt
    15181 acttaatggt ttatcttgag aaaaaatggg cggttcgttc atagaaggag gggaaataca
    15241 tggattttag cggcgctttt tcatggatga atatccgttt tttattggaa ggattaaagg
    15301 ttactgttga agtttcattg ttttcgatca tttttagttt cttgatcggt ggtctgacag
    15361 gtgtgattcg ttttgcgaat atccctttct tttccaaaat ccttggtgta gtgattgata
    15421 ttatacggaa tcttccatta ttattgatca ttttcttcac ttactttgcc ttacctcaaa
    15481 tcggcattca gatgaacatc ttctggtccg ctgtcgcagc attaacgatt tttgagtcag
    15541 ctatgttgtc ggaaattttc cgagctggac tcaatgctgt tccaaaagga cagatggaag
    15601 caggattatc aacgggactg acttatgtag aatcgatgcg aacgatcgtg ttaccgcaag
    15661 catttaaatc aatgatcccg gcaattgtga gccaattgat ttcactgatc aaagatactt
    15721 cattagcagt aatcatttct ttacctgaac tttctcatca ggcacgtatt atttatggac
    15781 agaacaccaa ttatgtgctt ccgatgttca tcatcatgac acttatgtac ttcgtggtat
    15841 gttacgcgct ttctctgctt tccggttact tggaaaagag acggtacagt tattaataga
    15901 tgattctttt agcggttgtg gcaaactaaa gtcacaaccg cttttcctct ttccattcga
    15961 aaaaaagaga tttcgattgt tttaagcaag taagagcact tgaaaattca cgctaaaaag
    16021 tacacaatag ggaggataga atttattgaa aaaaaggatg atacgattga cagataatcg
    16081 ccctatcgga tttattgatt caggtgtcgg cggcttgact gtagtaaaag aagccctgaa
    16141 acaattaccg aatgaaaata ttttatttgt aggagacaca gcacgctgcc catatggccc
    16201 tagacccgcg gaacaggtaa tccagtatac ttgggaaatg acggattatc tggtggagca
    16261 aggaatcaag atgctggtga tcgcctgcaa taccgcaact gcggtggctt tagaagaaat
    16321 caaagctgct ctttctattc cagtcatcgg tgtgatcctt cccggtacta gagcggcagt
    16381 aaaaaaaaca caaaataaac aagttggcat tatcggtacg attggtacgg taaaaagtca
    16441 agcttatgaa aaagcactga aagagaaagt accagaattg actgtgacaa gtcttgcttg
    16501 tcccaaattt gtttcagttg tcgaaagtaa tgaataccat tcatcggtgg cgaaaaaaat
    16561 tgtggcagaa acattagctc ctttaaccac taaaaaaatc gatacattga ttttgggatg
    16621 cacccattat ccattattac gccccatcat tcaaaatgta atgggagaaa atgttcaact
    16681 gatcgattct ggagcagaaa cagtaggtga agtatctatg ctgttagatt atttcaattt
    16741 gagcaattca ccgcaaaatg gtcggacatt atgccagttt tatacaactg gctctgccaa
    16801 acttttcgag gaaatagctg aagactggct tggaatcgga cacttaaatg tagaacatat
    16861 cgaattggga ggaaaataag gatgcgtcat gacgggagaa aagcccaaga gcttcgcaaa
    16921 ctggagcttc aaacaaatgt tttcaaatat ccagaaggat cggtagtgat ccgttttggt
    16981 gatacaacag ttatttgttc tgcaacaata gaaaattctg ttcccccttt tttgagagaa
    17041 acaggaacag gatgggtgac agcagaatat agtatgctgc ctcgagcaac gagtacgaga
    17101 aatcgtagag aaagcagcaa agggaaactt tctggtagaa caatggaaat ccagcgtctt
    17161 attggtcgtt ccttgcgtgc agtagtagac ttggaaaaat taggagaacg tagtatcatc
    17221 gtagactgcg atgtgatcca agcggatggc ggtactagaa cagcaagtat cactggggca
    17281 tttgtggcat tgaaattagc cattgaaaaa cttttaagag aaaaagaatt gtcagaagat
    17341 ccaataaaag aacatcttgc tgcagttagc gtaggaatct taccagatgg gacttgcgtc
    17401 acagatttgg attaccaaga agattccgca gcacttgtag acatgaatct tgtaatgaca
    17461 gaatctggaa aattcgtgga gattcaagga actggagaag aagcaacgtt tgatggtgaa
    17521 cagttgaatg aaatgctctt ttttggaaaa aacgcgattg aagatttgat caaagagcaa
    17581 aaacatgctt tgctaacaga gtttgctcaa aatgatgagc ggatagaaga aacaaaaaca
    17641 atcatcattg cgacaagaaa tccaggaaaa gcagaagagt ttcgaaatat gttcaaagaa
    17701 gcgggatatc atgtaaaaac attacttgac tatcctgaat tgccagatgt ggaagaaact
    17761 ggcagcacat ttgaagaaaa tgcccgatta aaagcagaaa cgattgcaca attgcttgat
    17821 cagccagtct tagcagatga ttcaggtttg aaagtggatg ctttaggcgg tatgccagga
    17881 atctattctg cacgttttgc aggggaacaa aaaagtgatg cggggaataa cgccaaatta
    17941 ttatatgaat tgacagatgt gccggatgaa aaaagaacag cacaattcca ttgtacgtta
    18001 gtattcgcgg cacccaaaaa agacagcctt gtagtagaag cggaatggcc cggtcgagta
    18061 gcccgtatac catctggcga aaatggcttt gggtatgatc ctttatttat cccagaaggt
    18121 aagaaacaaa cagcagcaga attatcgagc gaagaaaaaa acaagatcag tcatcgggct
    18181 caagcaatga aaaaattgag tgccgagtgg aaacaatggc tggaaggaga gagatagatg
    18241 cggtatttag tagtaagtga taatcatgga gacagagata tcctcgttga cttagtagat
    18301 cgttatcgtt ctgaagtgga tatctttttc cattgtggtg attcggaatt agaagcttct
    18361 gacccattat gggatacatt ccaagtggtt caaggtaatt gtgattatgg accaggattc
    18421 gaacagaaaa aagtgatcca aactggtcag gatacagtat tcatgactca cggtcattta
    18481 tcgaatgtcc gatttggttt gacacagtta gcaatcgaag ctcgagctgc aactgccgat
    18541 atggtgtttt ttggtcatac ccatcaaata ggctgtgaga tggatcagaa aatcctgttt
    18601 ttgaatcctg gtagcatttc tcagcctaga ggcccgatcc aaatcccatc atatgctctt
    18661 atcgaaagtt tagatgacca aatttctgtt caatactata accgtgccca tcaggcagtt
    18721 gaagagttgg ccttttcttt ttcgagagag cgctgaataa aaaaactcta atttttaata
    18781 aaaataagct ttattttatg aattttgcat ccggtttcgg taaaatagta gtgttgaatg
    18841 aaatagaaat ggaggccatc agatgattgg acctatagta agagaaatgc tgctggaaaa
    18901 tcaggaaacg tttttgattc ctgcagaaaa tgtggcaaat gttatgtatc atcatccatt
    18961 gtcccatggt ttacttgtgt tatcgaaggt cggttattcc aaaattcctg tattaggaaa
    19021 agatgatcac tttgtaggac tagtaagttt atcagatgtg gtcaataaaa tgattgactt
    19081 gcaaacaatc agcatggacc cgttagaagg actaacggtt gctgatgtga cagaaacaga
    19141 tgttccgaca attgatgaaa attgggaact agaagaagta ctccatttgt tagtcgattc
    19201 gcctttttta ccggttgtca cagaagatcg gattttcaaa ggaatcatta caagaaaaga
    19261 gctgcttaaa gccgtaaact tcatggtgca tgaattagaa cgtcgtaata tattgtctcc
    19321 gaaaatcgaa gaattaaaag aacaaatcga tatcgtgagc taaagaacga gaaactaaaa
    19381 gataatcaaa aaataagtag aaaaaacagc gtaccagttt tgactttatc ttgaaaaaga
    19441 ggctgggaca aaagcgttta actccaagaa ataagaagga attcacgaaa attgcttttc
    19501 aaatttttgt gaatttcagc ttatttccga aggagttgct tctgcttccg ccgtttatac
    19561 gtgtttagag cgtgggacaa aagtgttctt tacttttgtc ccacgctctt tttagtgaga
    19621 ttcgttgcat cgtttgtcca aattattttc cgacagcaat tggactgttc ggaatcgtga
    19681 aaccagaagc tgtcaattct ttgacatagg agctcaagaa ctcttctttt aaggcatatt
    19741 gttttccgtt caatgcgtag cagaccgtac gaatcgcaaa attgctgttt ccaagatcca
    19801 ctaatccaaa gatagaaggt tcagtttgga gctcttcttg gtatttttca gctaatgtac
    19861 gatttactcg atcaatgatc tcatagattt tgtcatatcc ttcttcagga acaatccgaa
    19921 tatctaaaag tacttgcata tttgcacgag agagattgct gatcgtggtg atgtttcgat
    19981 tcgggatgaa atggacagta ccatccgttg ctttcagctg aagggtacgg ataccaacag
    20041 atacgacggt tccttcaatc gttaagtttg ctagcttgat gtagtcccca acatccatct
    20101 gttgttccat gataataaaa aatccggtaa taacatcact catgaaccct tgggcaccaa
    20161 gaccgattgc cacacctgca attccggcac cagccaaaag tgatccgatc ggcacaccga
    20221 tagcagataa aattgcatag ataaaaaaga aaccaagggt ataatgaaaa atagtattta
    20281 aaagactatg taaagtcttt aagcggctct cgtttagaat ggctttttta gaatactgat
    20341 taaaagactt atcaatcaga tattttccca cacggtgaag aatccaaaaa aggacgatca
    20401 aaaaaatcag atataaactt ttttggataa aggcagccaa aatggcgtcc caatcaatcc
    20461 cgtcccaaaa ccgctgaaac gcatttactt tgctgacagc ttgatctgtc aatgtctcgc
    20521 cagaagagtc tgtcgtaccg gcagttgcta ataatgaaaa cataaaatcc tcttttctaa
    20581 aatttgattc tttctgtatt gtaccaagaa gttttcaaaa aaggaaatag caaaaataac
    20641 tataatgaag gaaaaaagct gaaaataaaa tgacaaataa tatggataat gataaaatgt
    20701 ggagaaaata acgaaggtgg taagtatatg caaatcgatt ggaacaatct aggtttttct
    20761 tatatcaaaa ctccttggcg ttttattgcc aaatggaaag acggacaatg ggaagaagga
    20821 gaattgacag aagataatta cctgacgatt catgaaggct ctccagcgct tcattatggt
    20881 cagcaatgtt ttgaaggtct aaaagcttat cgtcgaaaag acggcaaggt caatctcttt
    20941 cgacctgaac aaaatagtcg acggatgaat caaagtgcta gacgattgct gatgccaatg
    21001 gtccctgaaa agatgttcgt tgatgctgtc aagcaagtgg taaaagcaaa tgaagatttt
    21061 gtgccgccct atggaacagg cgctacatta tacttgcgcc ctttactgat tggggtcggt
    21121 gaaaacattg gtgtccatcc agctccagaa tatttatttg tcgtcttctg tacgccagta
    21181 ggagcgtatt tcaaaggtgg actaaaacca acgaatttta ttgtatctga ttacgatcgt
    21241 gctgctccta aggggactgg tgcagcaaaa gtcggcggaa attacgcagc gagtttactt
    21301 ccaggaacag aagcaaaaga actggattac tcagattgtg tttatcttga tccagccaca
    21361 catacgaaaa tcgaagaagt aggtgcagca aatttctttg gaattacaaa agacggaaaa
    21421 ttcatcactc cgcaatcgcc ctctatctta ccaagtatca cgaaatattc cttgttgcag
    21481 ttagctgaag aacgactagg actgcaagca attgaaggag atattttcat tgatgatcta
    21541 gatcaatttg cagaagctgg tgcatgtggt acagcggctg ttatttctcc aatcggtggg
    21601 atttatcaca ataacaatct tcatgtattt tatagtgaaa cggaagtagg accagtaaca
    21661 aaacggttgt atgatgaatt gacaggtatt caatttggag acatagaagc accagaaggc
    21721 tggattgaag tcgtggaata atttcaaagt aatgagttga aaaaaacgta aaaagaaaaa
    21781 atcaggaaga gactttaaaa agttttttcc tgatttttat atgttttact ttaaagaatg
    21841 caacaaaagt ttatgaaaga tggacacctt tgtcaacgta gataatatca ccgatgattc
    21901 cgctggaaag tgggctgatt aagaaagcac aagcattacc gacttcttcg atcgtcacac
    21961 cttgctgatc aggcgtacga gattcagata actggatcag tttttgatag tctttaacac
    22021 ctgtgacagc taatgttttg attgccccag ctgagattcc attgacccga attcctttag
    22081 gcgagaactc agctgccaaa taacggatag cagcttcaag agaagctttc gcaataccca
    22141 tcatgttata attcgggatt gcgcgttctg aacctagata agtcatgcta acgatcccgc
    22201 tgtttggtgc aagaaacggt tgtgcgtatt ttgatacagc aattaaagag tagctgctga
    22261 tatcttgagc taattgatag ccgtctctgg aaatatcaga cacatttccg gacaattctt
    22321 ctttgttggc gtaagcaact gcatggacaa ggccgtggat ctccccagca tattcaccga
    22381 tggcattcat tgctttttcg atgctttcgt cgctagaaac atcacattct acagtgaatg
    22441 cttcttcgcc gaccaatttg actaatgatt ttttcattcg ttcattttgg taagtataga
    22501 taatttctgc cccttgtttc aacattgctt cagcacaacc ccaagcaatg ctttttttgt
    22561 tagcgacgcc cataatgacg attttttttc cttctagaaa tgacataatg actctccttt
    22621 gtttctgata aaaatttgat agtcgtaaaa aaatagagaa taatagctaa aataacttct
    22681 tgatttctta attattagtc aaaatgtttt gattgtcaaa ctattttttc ttaaattaaa
    22741 aaaagattgc taaatattta ggaatatgat actttgatag tcaaagtaat gaaaatggca
    22801 attgcctgaa agaaggagtt taattatgtc ttacgtatta tctgctactg aagtaatgga
    22861 attgattcct aatcgttatc ctatttgtta tattgattat gtagatactt tagaaccagg
    22921 aaaaaagatc gttgcgacaa aaaatgtaac aatcaatgaa gattttttcc aaggtcactt
    22981 tcctgacaat ccagtgatgc cgggtgcgtt gattctagaa acactggcac aagcaggttc
    23041 gatacttatc ctaaaatcag aagagtttct aggaaaaaga gcttatatcg gtgggatcaa
    23101 taaggccaaa ttccgtcaaa aagttgttcc aggagatgtg ctgaaatgtc attttgaaat
    23161 caccaaaatg aagggatcag tcggtacagc gttatctgca gcatatgtag atgataaaaa
    23221 agtctgtgag tgcgagttca cgttcatcgt caaccaaaat gcttgactat aaaacctgat
    23281 atagcaaaac aaaaggaagc ttacttgctt tttatgtaaa aagaagacgc ctgaatccta
    23341 gcatacgttg tcatctatgc aggattcaga cgttattttt ttatttttgt tttgcttctt
    23401 gcagtatatt gattttatcg atttttactt cttcttttgg tttgtctgaa ctatctgttt
    23461 cgatatttgc aatagcatcc acgacatcca ttccttcaat cacttggccg aagacagtat
    23521 aatggccatc tagtgaagga taaccgccgt ttttataagc gtcgatgatt gcttgaggat
    23581 agtcgtcttt taaaagacca tcatgcatat catctgtatt ttgaacgata aagaactggc
    23641 tgccgttact gttaggatct tgtgagcgag ccatagataa cgcacctctt atattataaa
    23701 ggtggttgtt gatctctgtt tcaaatcctt ctccccaaat gctttcccct ccagtgccat
    23761 cgcctttagg atcgccacct tggatcatga aatctttgat gacgcgatgg aaagtcagtc
    23821 cgttgtagta accgtctttt gcatgtgtca taaagttttc aaccgctttt ggtgcgtgtt
    23881 cgggaaataa tttgattttg attgtgcctt tattcgtcac catttcgacc agatcttcat
    23941 tttcggcgac atctttcgtc aattgtggta attccaatgc gttcaaatcg acgctactgg
    24001 cagtcgtgct ttccgcagta gatgtacttt ttgaactttc tgtagacgaa ctagaagaag
    24061 tcgtgttgtt tgcagaacaa ccagcagcaa acagcaatgc tgctgctaaa ctaatactta
//
LOCUS       DAZABD010000045        22440 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000045 DAZABD010000000
VERSION     DAZABD010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.045"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            144..542
                     /locus_tag="ACHALG_001837"
     CDS             144..542
                     /locus_tag="ACHALG_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFN2587221.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            526..1221
                     /locus_tag="ACHALG_001838"
     CDS             526..1221
                     /locus_tag="ACHALG_001838"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFN2587222.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(1815..2702)
                     /locus_tag="ACHALG_001839"
     CDS             complement(1815..2702)
                     /locus_tag="ACHALG_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFN2587223.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            2887..3726
                     /locus_tag="ACHALG_001840"
     CDS             2887..3726
                     /locus_tag="ACHALG_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFN2587224.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            3922..4896
                     /gene="sgrA"
                     /locus_tag="ACHALG_001841"
     CDS             3922..4896
                     /gene="sgrA"
                     /locus_tag="ACHALG_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HFN2587225.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            5194..5550
                     /locus_tag="ACHALG_001842"
     CDS             5194..5550
                     /locus_tag="ACHALG_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587226.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            5595..6710
                     /locus_tag="ACHALG_001843"
     CDS             5595..6710
                     /locus_tag="ACHALG_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587227.1"
                     /translation="MKNYHLSCSLKKGNPIEGEEYRIRYFFHLMYWQSFDENHSDILS
                     SIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSITYVPEEMNKFVHPL
                     ITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYLEEDLLDTPDKVQES
                     WGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGTKKKVDAFGKSAEDR
                     DFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCMLVRDIFIKHEQPVIF
                     YIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYPIDKKAYDQTNTQIF
                     YWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6736..7176)
                     /locus_tag="ACHALG_001844"
     CDS             complement(6736..7176)
                     /locus_tag="ACHALG_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFN2587228.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            7336..7842
                     /locus_tag="ACHALG_001845"
     CDS             7336..7842
                     /locus_tag="ACHALG_001845"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFN2587229.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            8010..9923
                     /locus_tag="ACHALG_001846"
     CDS             8010..9923
                     /locus_tag="ACHALG_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFN2587230.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            9928..10929
                     /locus_tag="ACHALG_001847"
     CDS             9928..10929
                     /locus_tag="ACHALG_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFN2587231.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(11131..11337)
                     /locus_tag="ACHALG_001848"
     CDS             complement(11131..11337)
                     /locus_tag="ACHALG_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HFN2587232.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(11983..12831)
                     /locus_tag="ACHALG_001849"
     CDS             complement(11983..12831)
                     /locus_tag="ACHALG_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFN2587233.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            12995..13897
                     /gene="gnd"
                     /locus_tag="ACHALG_001850"
     CDS             12995..13897
                     /gene="gnd"
                     /locus_tag="ACHALG_001850"
                     /EC_number="1.1.1.343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase
                     (NAD(+)-dependent, decarboxylating)"
                     /protein_id="HFN2587234.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            13899..14900
                     /locus_tag="ACHALG_001851"
     CDS             13899..14900
                     /locus_tag="ACHALG_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-isomer specific 2-hydroxyacid dehydrogenase
                     family protein"
                     /protein_id="HFN2587235.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            14897..15181
                     /locus_tag="ACHALG_001852"
     CDS             14897..15181
                     /locus_tag="ACHALG_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFN2587236.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            15214..16011
                     /locus_tag="ACHALG_001853"
     CDS             15214..16011
                     /locus_tag="ACHALG_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFN2587237.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            16025..16858
                     /locus_tag="ACHALG_001854"
     CDS             16025..16858
                     /locus_tag="ACHALG_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFN2587238.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            16876..17733
                     /locus_tag="ACHALG_001855"
     CDS             16876..17733
                     /locus_tag="ACHALG_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFN2587239.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            17755..18264
                     /locus_tag="ACHALG_001856"
     CDS             17755..18264
                     /locus_tag="ACHALG_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFN2587240.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            18301..18702
                     /locus_tag="ACHALG_001857"
     CDS             18301..18702
                     /locus_tag="ACHALG_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587241.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            18814..19167
                     /locus_tag="ACHALG_001858"
     CDS             18814..19167
                     /locus_tag="ACHALG_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587242.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            19201..19644
                     /locus_tag="ACHALG_001859"
     CDS             19201..19644
                     /locus_tag="ACHALG_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFN2587243.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            19661..20746
                     /gene="uxuA"
                     /locus_tag="ACHALG_001860"
     CDS             19661..20746
                     /gene="uxuA"
                     /locus_tag="ACHALG_001860"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFN2587244.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            20739..21452
                     /locus_tag="ACHALG_001861"
     CDS             20739..21452
                     /locus_tag="ACHALG_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFN2587245.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(21659..21808)
                     /locus_tag="ACHALG_001862"
     CDS             complement(21659..21808)
                     /locus_tag="ACHALG_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587246.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            22204..22404
                     /locus_tag="ACHALG_001863"
     CDS             22204..22404
                     /locus_tag="ACHALG_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFN2587247.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         7738 a         3449 c         4638 g         6615 t
ORIGIN      
        1 tccatacatc cattgtactc tgagaaatca cacaatatcg aaacaaattc cgttaggaat
       61 gtgttattca ataaatttta taatctagaa acattttgaa taaaaactat tagacaaatg
      121 atattttacg aggaggaaca gagatgacga tctttgaaac ggttgtaaca gatgttggta
      181 tagaagcaga agtattcagg aaagaaaaaa taatgatttt atttggagat gaagcaccgg
      241 aaacattaac agattattgt tataagattg aattgaatca ggtaagaaaa gaaataactt
      301 gtgatatgaa gctctgtttt gataaacaaa tgtatgtaat cacagcagta ggagatgttg
      361 tccaaactaa tttgaatgaa ttaggacata tcacgataaa atttgatgga tctcatgagc
      421 cggaacttcc aggaacaatg tatgtagaag ataaggaaat gccgcattta agaaagggga
      481 caatcattaa aatcgatcaa gaggatcaga atgggggaaa gctatatgga aatcatgctt
      541 gatacagcaa atacaaaaag tatttattct tgtcaacagt ttttgaattt aacaggcgta
      601 acaacaaatc ctacaattct gaaaaaagaa ggagcgtcaa acttcttctc gcatatgaaa
      661 gaaatcaaaa aaataatcgg acagattcct atccatattc agacaatagg aagaacggaa
      721 gaagaaatga tagaggatgc tcgagttatc atagaagaac tgggaaaaga tacgtttatc
      781 aaggtgccgg tgaatcaagc aggccttcaa gcgatcaaaa agttgaaaaa agaaggttat
      841 ttgattaccg gtacagctat ttacacagaa ttacaagggt atttagctat taataatgga
      901 gcagattata ttgcacctta ttataacagg atgcaaaatc aaggcattga tgctatgaaa
      961 gtgattgatt cgttgcggac tgagatcgaa cgtacgcaaa gaccaacaaa aatattagca
     1021 gctagtttca aaaattgtga acaagtcact acagccattc gatctggagc tcatgcgata
     1081 acgttaggta aagagctact agatcaagta ttagagatgc cctcgattga taaagcagtg
     1141 aggggcttcc aagaagattg ggtgcaaact ttccaagcag aaacgattgg tgaactaaaa
     1201 aaggtcgctg attacttctg aatcaatttc tccataaaat aaacgatagc cttctcgaaa
     1261 actcgaggag gctatctgac gattcaatat tcattaattt aaagtttggg tgactattac
     1321 taactattac ttatttctca tttaaacgat gtattcccta acctctaata catccttgta
     1381 actctgaagt tacgccagtt ttgaataaat ttcataaaaa caaatgaaaa gtgtcgcata
     1441 aaaatatctt tcgtgtttct ctagtataac agaataagaa gaacaagtcc agaagggata
     1501 tttttagtct tttttaaaat aacttttcag tcgagtttga gtttatgata gttgtttttg
     1561 aagcgatacg gcaagtgaaa aaatagggaa gcatgaaaga atctacttaa tggcgcattg
     1621 attcatgtga tagaaataaa aaactttttt aaattatatc ctcatgattg taacaaatcc
     1681 agtgatttgt tttggtacaa ataaaaaaag gccccgatga agttgtggca aaagtggttt
     1741 gcctcaaaaa taagaaggaa tttccaaaaa tcacttctca aattttgcca catccccaat
     1801 tgggtcaaat ttgtttattt gaatatattt tttacataag gatctttaat agttacattg
     1861 tcttttttca tcactttacg gattgtttta tccatgaatt ccgtatcaag agtttttgct
     1921 tcggtagcaa ttttttctag ctcagatttg tatttgtctt tattagaacc tttatcctgt
     1981 ttttttacca tttttactag ataatagctt tgctgataag tcgttggatc tgttacagga
     2041 atgatatcag aaacttcccc gttttttagt ttaaatgctg ctttttttac ttcagtagga
     2101 atctctggat tggttgaatc aaaattcatt tttccatcag tttcctttaa tgttttattt
     2161 tttccataag cttgaacaag cttggagaaa ttttctcctt catttgcttc tttttttgct
     2221 ttatctgctt cttctttagt cgaaaataga atgatctgtg tactaacttc tggatagaat
     2281 tctttccaag cagtgtctag atctttatct gttaatttta cattggcttt caaaccgtat
     2341 tggaaagcca gcatctgttt aaagagtttc ttatatgtct cttctgtttg gccaactgct
     2401 tttaattgtt ccttgtagtt tggaccgagt tttttgattt gttcattata ttcttttgtt
     2461 acttcctttg aagaaatctc ttttccgtat acatcatcaa atgctttgaa gacaatcatt
     2521 ttttttaata gtgtttgatt atttgattta acttttgggt tatcataaaa atcctgaact
     2581 gtaagtgttc cacctttaaa cgttgcaatc gtttcattgg acttgctaca acctgagaat
     2641 agtaaaattg cacatgctga aaggcacata attagtagtt gttttttcaa aaaatttccc
     2701 atcctttcct tattaaaaac agtctaacag aagaattagg gagacagtct ttcattttcg
     2761 taaggttgct gtagatattc ttagttcgta aaaatctatt ctctctatga tggagaacat
     2821 ttacatagtt ttcacgagac aagaaagaaa agggttgtta aacttaatat aaaggaggga
     2881 tgaaagatga tcaggattat tcggttgatt ggatctgttt taatagtctt agttggtttt
     2941 gttttaggaa ttcttgtcaa taatgcactt gcggatatgt ttattggaaa tagtgaatgg
     3001 attggagcaa ttgccggtac ggtcggacat ttagctgttt ttttactagc tttatttctt
     3061 gctagtaagg tcgaaggaaa aagaatagaa gattatggtg tttctgttcg aggaaaagac
     3121 tgggggtatt tagcgggagg tctcgttgtt ggaattggag tgtttctgct tattactagt
     3181 ccattatatt tgacgggggc gtatcggtta gattcaggaa acgcaaacat tgtgccgctg
     3241 ataacgagtt ttattttatt tattgcagtg ggggcatcag aagagctttt attccgtggc
     3301 ttttttcagc atcagatgct tagatttggt ccgttaatag caatgattgg atctgctgca
     3361 ttatttgctc ttttgcacgg attgaatcct aacatgacat ttctggcagt tttcaatatc
     3421 tttttagcag gttgcttttt cagcgcactt atctacagta caggaagttt atttacggct
     3481 attggtgctc atattacttg gaattggaca caaggagcta ttttgggtat accagtcagc
     3541 ggaacgcaag aacaaggttt tttctccact ctgatccaat cgcaaaatac aataattact
     3601 gggggaaact ttggggctga agcatctata tcaactacat tattcttgtt agtcttgatt
     3661 gttggtttgt tactctatgc atataaaaca aagcggatga agaaatacac tgctgtttat
     3721 caataaaaga tgaagaaagc aaggctgata tcggtcttgc ttttttaagt ctgtcattga
     3781 ttaaacgaaa aagtaaacaa attcaagcga atacacgaca tcttttgtta gattttcaca
     3841 actgactgtc gtgttctcat cattttcatt ttatataatt cctttatcaa atagaaggta
     3901 attatatgga gagtgataga aatgaagaaa acagcgaccg tattatttgt tattcttggg
     3961 ctgagtataa ttgcagcaat gattggaatc agtacttctg caaatgaacg ggcaaatgag
     4021 aatctttcat ttactgtgaa aactgatcgg attgtttatg acatgacaca acaagtcatt
     4081 actattccag tcaaaccaaa caaaagcgtg aatgctagtg atgtacatgc tgttctaaca
     4141 tatggttggg atggtaatgg aagttcagaa aaagttattg gggaagttta tttaaaagac
     4201 gtacaatgga ccgctggtat tgagtataca ataatgattt ctgcagaact atcgattgat
     4261 gagatcaaga gtaaagataa agttgatttg attgtttttt atgatggaca gatgacgatc
     4321 acggagaatt tgaaaccaag cagttggaca gttgttggac cgccaagcag tacggatagc
     4381 acaagtagtg aaagcagtac agaaaacacg agcggtgaaa gcagtacaga gagtacgagc
     4441 agtgaaagta gcacagaaaa cacgagcagc gaaagcagta cggagagtac gagtagtgaa
     4501 agtagcacag gaagcacgag cagcgaaagc agtacggaga gtacgagtag tgaaagtagc
     4561 acaggaagca tgagtagtga gagtagcaca ggaagcacga gcagcgaaag cagcacagga
     4621 aaagtaaata atgaacttga tttggtacct atcataccaa ctaaggaaca aaagagaaat
     4681 gcagggagca acctaaaaag tgtgctaaaa cagcctgatt ttgtttctcg tggaattgat
     4741 catcaagaaa aagcgaaaga aacagataat agaaaaagcg atttaccaga aactggtagt
     4801 agaaccttga acaagctaat tacatggatg ggtgtgttac tcattttgat tgtaggagca
     4861 agctattttc gttctctctt ccatcgagtg aaataaagaa taaaagcaac agatctaaaa
     4921 ttgaataaaa cgatagattt ttgagtgaat atttcattcg agagtgctgt attgcctggc
     4981 gaagttgtag ctttgccaga tctatttttg tttactaaca tatttgagaa acaggaaata
     5041 acaggaacac aaatggaaaa taagagcaag aataattgac aatgcacact gcgcatggta
     5101 gtatctctga aaaatcagta acgaatctat ttgtacgtta ttcatgtagc tgtcggaata
     5161 agtctaaata atagataaaa aggagtatgt actttgtttg aagaatttat tgatatcaat
     5221 gaaaggcaag tatatcaatt tttgaactat tgttatgaaa gagacgagaa attatatgtg
     5281 gtgaaggata tcgctttaga tctaaactac accctggcta agatgaattc ggtgattcaa
     5341 caagcagaat cgttttgcga aaggtatcca gaatataaat tatcttttct ttcagaaaac
     5401 aagatgataa aagtcgagtt ttcttctcag tttttactga gtaaagttta ttctatactt
     5461 ttagagggaa cgattggtta tattttactg gattcacttt acaaaggtac ttatcaatcc
     5521 ttagaaaatc ttagtcaaaa aattatataa gtttgcgtac gattcaaaga aaattaagag
     5581 aacttaatac tattttgaaa aactatcatt tatcttgcag cttgaaaaaa ggtaatccga
     5641 tagaaggaga agaataccgt atccgatact tttttcactt gatgtattgg cagtcattcg
     5701 acgaaaatca ttctgatata ttatcaagta taaaaaataa acgtcaacta ttagaaaaat
     5761 ttgcgaaaca tgcgccatat acgcgtagta tagatagaaa gaaatggatc agcctactaa
     5821 gaatttcgtt gcaacgaatg aaacaaggcc actcgattac gtatgtccca gaagagatga
     5881 acaaatttgt gcatccattg ataacaaaag aagagtttac gcattacata ctagagcctt
     5941 tttttgaagc aaacttctgg tcgatgaatc acatcactcc aacagagcaa gcttaccttt
     6001 attttatgtt tggtgtaatg aacacctatt tggaagaaga tttgttggat actccagata
     6061 aagtacaaga aagttggggc tggattgaat cgtctattgt agaaaatttt gaaaaaaact
     6121 tcaagatttg ttttacaact tcagaaaaaa attatctata tgtcaatttg gcaatgatcc
     6181 atttgtattc aagggttttt gggacaaaaa agaaggtgga tgcttttgga aaatcagcag
     6241 aagacagaga ttttcagcat atatttccag tgatgtatcc aatcatgcaa cagtttttta
     6301 gtggggtagc tcgcagaatt ccagagctta atacttatta tgaaaagaac agacggttga
     6361 tttttcaata ctgtatgtta gtcagggata tttttataaa acacgaacaa cctgtcatat
     6421 tctatattca atcaaagtat ggtaaaacac aggaattatg ggcaaagaaa aggatattat
     6481 ctatgactga acgcccaata aaatatagtg catccgctga tcagttacct gatattgtca
     6541 ttagcgatta tcctatcgac aaaaaggcat atgatcaaac gaatacacag atattttact
     6601 ggaatggaca acctacaaaa aatgattggg cgcgtctgaa agctgaaatc ataaaaatca
     6661 ggaatgagaa aaatagatcc atattggaaa aattggattt cgttaattaa gaaaagcaga
     6721 ttgtttatgc caaaattaag acattgcaaa aagattattt tctttaaaat cgacaagatg
     6781 accagaaagc aagagttcgt tgagctcttg ctttctttta gttttagaaa ggaacatcct
     6841 gattttatct tcgctaaatc cagtcaaaag ttttgcagat tggtcgtaga taatcggttt
     6901 tttcaataaa gaaggggatg tacgaatctc aatatatagt tcactcaaac tgagttcatc
     6961 tattctgtga acaagatttt tataactaat agaatgagta gagagcattt cggtcacgcc
     7021 gttgtttgtc aattttagta agtggataaa ttcatttttg gttagtggtt gacgaattaa
     7081 gttctgttcg ataaacggta tatcatggac gataagccaa cgttttgcgc gtcttgaaga
     7141 cgagcagcca gaacctgtat acaatttaat catcatacat tcctccaata gctaagtatt
     7201 tatgaaataa taacatgaag aaatagtcga aaagtgccat ttagaggtgt aaaataagaa
     7261 aatgttgtca cgtataatgg aactagctag ataagaaata ctattaaatc aagaaaaaag
     7321 aaagaaggca caagtatgtc aaacacagcc gacgcaatag tagtgatcga tttacaaaaa
     7381 ggggtatgtt ttgattcttc gccgatacac gaattagaaa cagtgacagc attagtgaat
     7441 aaaagaatca aaacatacga aacgattggg aaaccagtta ttttcgttca acacgaagat
     7501 gctcaattag tgccacaaac aaaaccatgg gagctattgt cggaattatc tattcctgag
     7561 cacagttatt ttgtgagaaa aacccatgcg aattcatttt ataagactaa tttgagtgtt
     7621 ttattaactg aattggactg tcattcgatc gaattatgtg gggcacaagc agagtattgt
     7681 atagatacga ctgtgaagtt tgctcatgga ttaggatacc agcttatgat gtgtagagga
     7741 gcaacgacaa cttatgataa tccttttatg aaagcaaagg aaacaatcgc tttttacgag
     7801 aaaatttggg ataaacgatt tctgacattt ttggagcaat aagttgtttc ttcgtttaaa
     7861 aaaagcagaa tggatactgg tttatccatt ctgctttatt acgtaaaatg agaaaataat
     7921 cctgttctat ttttatggga taaaccattt attttttgta taaaaaatgt ttgcgtttac
     7981 atataattct cctagaaaca taaaaaggag tgaatagctt gagaacaaaa caagtattaa
     8041 agggagcgtt ggtcatctta tttgctgtaa gtacaagcgt cccgcaatgg acagtatatg
     8101 gagagagcat ttcttccgaa tcggaatctc gggaggtaga aaccacagaa actactgatg
     8161 ctggaaatga aacgaaatcg agtacttcac aaacagaaac aaatactgaa acaacggttg
     8221 agacatctgt tactagcaca gatcaagaaa cgaccgctac cacagataac caacaaacag
     8281 aagaagcaac agctacaaaa atgtcaaacg acgaatcctc ttctgctaaa gtaagtgaca
     8341 aagagaatgg gacagaaaat gatgatatcg gtaaaggtga aaatttcaat ggtaaagaat
     8401 atttgaatta cttcggtaaa cagatgacag aaatacttaa tggaccaaca gaagacatca
     8461 agtcaaaatt aatagagtta ttagtcactg agcttgctgt taatgattat ggtgtagata
     8521 aagagattat caaacatctg attgctactt atctattgta cgatatcgct tttgcaaaaa
     8581 acagtgtgtt gatgtcgatg cttggaggta cagatgatgg gacaacgcct acagaaaaca
     8641 caggcttcta tccaaataca aaacaacttc atcaaactgt actaaacaaa caagatggca
     8701 aaatgatgga tgtatatgca tattatgtcg atcaaggttc agataaaaca gtcatgatcc
     8761 atggcggatt tcgagggaat tggaataacg ggatcgttac agaagaatac aatgattttt
     8821 ataaggccgg gtataattta ttatttgttg attccagagc gactggtaat agcggaggcg
     8881 attatgtgac ctatggacaa tatgagtcag atgacgtcct ttactggata aatcaagaag
     8941 ttcgagagcg gccatctcaa aagattttac tttatggtgg ttcgatggga gcagctacga
     9001 tgatgtctgt attagcgaaa gatattcctg tcaatgtcaa aggaattatt gaaaattgcg
     9061 gatttgcatc aattgatgaa caactacgtt ttacgtacag ccagacagta gcccccgcac
     9121 taccggatgc cataaaaaat cagttagaca tcattgggga tcaagagcat gaagatttat
     9181 ttatgggcct tttgaaacaa tactattttg atcaggagat gcatttggat acaaaagctg
     9241 ctctacctac gattggaatg tcagattcat tgcctaaatt gattatccat ggaacagcag
     9301 atgatgtagt acctgtgtct aacgcgcaaa aattatatga gttatcagga gggtataaag
     9361 atcttttatt agtagaagga gcagggcatg gaaaggcaca ggaagttgac catgctgcct
     9421 acacgaaaca tgtaacggac tttttgaaag tagtgttcca tgatcagata aacgtaaagt
     9481 atgtggatga aaacaataaa agtctactca aagaccagga cgaaatccag ctttacggag
     9541 cttatggaga aaattatatg acagagcaaa agacattcga aggatacgaa ttagcaaatg
     9601 ttgaaggacc gactgaaggg atttttaatg agacgatacc aactattatt ttctattaca
     9661 aaaaaatccc agtagctcca ccaaaaaagc aagatccgac agagaatgca gacaataagg
     9721 gaaagaatga acctcgagag gatcaatcaa gtgatgatac gcaaccgatt cagatgacag
     9781 tgaaagtaca aaaaaagaca gatgcaaaaa aaagaatgtt gccgaaaact ggagaaaaga
     9841 aacattcgct tctcatttat tcaggtggat ttttatctgt tagcacgatt atcacatgga
     9901 tttggcgaag aaagagaggg taaatgaatg aaatggttca agcgaatagt gattgcagta
     9961 ccagttatca tcgtagttat tttagtcact gtatttttga ttaatcaaat cacaccaaag
    10021 ccgattagct tgcttgttag aaaacaattt gacacaagtg aaaaagaaca agtatatgcc
    10081 cgaccagatg atttccagca aaaaatcgaa caaatcgata taaaaaaaga aatatcctat
    10141 ccttcaaagt acaaaaataa taatatggat atctacacac cgaaagtgga aaagaaaaaa
    10201 ttaccgattc ttttctggat gcatggaggc gcatatgtag gaggggataa aaatgattgt
    10261 cgtgattatt tggaatatct atgttcagac acgcagcaaa tcatcgtaaa tatagattat
    10321 gaacgatcac cggaagcaaa acatcctaca ccagttatcc aattgaacga agccattcaa
    10381 gcagctaaaa aagagataaa agatcaagca gattggtcga atatttcgat tggtggaaat
    10441 tcggcaggtg cacaaattag tggagaatat ttgttagcac ttcaaaatga agaaattaaa
    10501 aaagctgatc atcttgatcc gactttggag aacaagcaga ttacgaagtt tatttctttg
    10561 tctggtctgt tggatccttc tgcacttact caagtaagtg ataaaatctc tagctttctt
    10621 tatgaaaaat gtggatgggc ttactttgat aataaggact ttgaacaatc agagaaggtc
    10681 aaaagtttag ccttggtcag acatgcggat cgttggacgc aaaacacatt cttaacggat
    10741 ggaaatacaa atacgtttac gaaacaaatg gaagaagtaa cttctgtatt tgagaaagcg
    10801 ggggtgaaag tcacaaaagt agattatgcg gagaaaaatg ctgttttgaa ccatgaatat
    10861 cagtttgatt tcagcaataa acaagcgcaa gaaacatatc aaaagttagt aatgtttttc
    10921 aaggagtaaa gtcttatgat gcaaaagagg ttgtaatctg tcacttagct ccaagtcaca
    10981 aggagcaatt tcaataaaca agcttttcat ggtttatgaa tattgtaact tgtgactgag
    11041 gagttagaga tggcaagggg caccttttat tcgaaatttg ggaatatcac acgattattg
    11101 tcacgatctc ttttacgtaa attttataag tcagatttgt tgttttgatc gtttaaaaaa
    11161 agcaacaaat ccgaagcctg cggaggcaag aagggacaaa aagccgagtg gtaagcaaaa
    11221 gaatgctggt tccactaata ttccgttgtt attgatataa ggctgggaac aaagatgaat
    11281 agcaaataaa atcaatgcac tagcgaaaaa aagcaaagaa atatggatag ctttcataag
    11341 cagcacctcc ttttttataa ttgtatgata aatagattaa gaatctataa atgtgacaag
    11401 tttgttagga tgtgaataaa atgatcatct agcgaacttg aaacaaagat ggctaatgat
    11461 aagcaattaa tgattcagca atcagatagg tacaaagttt accatcatac gtctagacca
    11521 ataatagaaa atatgggaat ataatagaaa agatttagga tacccccaaa agaatttccc
    11581 caaaaattcg atgtatccta aactttttta ttttacataa caatcatatc aactgtgaaa
    11641 taaaattaca aatgataacc taaaatgagg ccaaaagcca gtgttatcaa ggaaatactt
    11701 aattcaccaa aatttttttt aattttttag aaaataagaa acaaaaataa taaaaaaaag
    11761 aaaagataaa ttttttacta ctgtatacta actttgttat cgcatctaac aagtaactcg
    11821 aataatacca atacaacctt ctccaaagat gcgatgacat acgttctaac ccaccgacta
    11881 ttgatgttct gtaccaccct tgcagaacca aaaaagccac taccaaatat ttggtagtgg
    11941 ctttttttgt tttatggatt tttcgctttt tctgttatgg tgctattctt tgatttcaga
    12001 aatagtatgt cgaactttag cgatagaacg acgtgattct ttttcgttgt aaaacatcaa
    12061 aatcacatat agagagtcca aaatgcttag ttgagaaata cgggaagcta aagcttcaga
    12121 tcggaattct gtttcttcag aaatagaaat aaaaacgaca tcgccaagtt tagcaaggga
    12181 agaattagct tggctagtaa taacgattgt ttttgctcca gtttttctta cggtttcagc
    12241 aatatggatt gtttcttttg attgccctgt gtgagaaatc aaaacagcac agtctttttc
    12301 cgtcaataaa gaagcttcca tcaattgaat atggtaatcc gtgctgtgag aagttgcgat
    12361 aggggaacgc aaaaatttat gataagcgtc agttgctaca atctctgaac cgccaacacc
    12421 gaaaaaatag acacgtttag attcattgat aatagtagcc gcttttttta gatccttttg
    12481 ttgtaacaac ttttttgtgt catttaaagt tgaaatacta gactcgaaca ccttttgcgc
    12541 catagtgagc tcagtatctg ttttctgaat attttcgtgg atagaaatag cagatagatc
    12601 attttcttgg atcaacatgg ccattttaaa atctttaaat ccattataac caagtttttt
    12661 tgtaaattga aaaaaagtag agtcagcaat gcccagttca cttgctaaat tgctaatagg
    12721 actatgactt actttgcttg gattttctaa aatataatcg gcgatagcct gttcttttgg
    12781 gctgaagtcg ttgtacattg ttttgatttg tagtcttatg gagacgctca tgagaattcc
    12841 tcattttcat aataagatta tcattttttc cgttctcatt aatgttaaag atacaaggag
    12901 agtaggaaaa aatcaaacaa aaaatatttt tgtaaaatct atttacaaaa aaattttttt
    12961 gtataatgag ggtataagaa atggaggcgc tatcatggaa attggtgtaa ttggtttggg
    13021 aaaaatggga ttgaatttag ctttaaactt aaaagagcac catcacaaag tacaaggact
    13081 cgacatttca agtacagctg ttagtcaggc aagagaaaat gacattcctg cttatcaaga
    13141 gatctctgaa tttctagcta cttttgagca gaaaaaaatc atctggttaa tgcttcctgc
    13201 tggagagata accgaaaaca tgttaagaaa actttttcca aaactcggac caggagacat
    13261 tattattgaa ggcggaaact cttattataa agattcaatc cgtcgagcag aagagtttca
    13321 gaaaaatgaa atcggttact tggattgtgg gacttctgga ggaatcgaag gagcacgaca
    13381 tcaagcctgc ttaatgattg gaggagataa agaaactttt ctatccgtgg aacaattatt
    13441 caaagatgta gcaattgaaa gaggatatct gtatgctggt gcatccggaa gcggacactt
    13501 cttgaaaatg atccataatg gaatcgaata tggaatgatg caagcaatcg gtgaaggatt
    13561 ccaacttgta caaaaaagtg agtatgcttt tgacttggaa caagtcgcac gtgtttggaa
    13621 tcatggttcg gtgattcgcg gctggctgat ggaaatcatg gaagatcagt taaacgaaca
    13681 tcccaattta agtaattatc ggggaatcgt ttctgcatca ggtgaagcaa aatggacggt
    13741 tgaaacagcg ctagaaatgg ctgttccagt tccagtaatt gcaactagtt tgttcatgag
    13801 gaatctttca caggaagaag atagtttttc agcaaaagta gtttcagcac taagaaatgg
    13861 gtttggcgga catgaaatag ttgaaaaggg tgagtaaaat gccagattat catatagcaa
    13921 tcgttaattc cagcagtttc ggaagagtct ttccacacca cttggaaaga ttaaagtcag
    13981 ttggagacgt tcatttcttt actgttgatc aagagattcc aggaaaagaa ctagcggagt
    14041 tacttaaagg attcaatatc attattgcaa gtgttacgcc attttttcga cgagattttt
    14101 ttgaaaacaa agatgaactg ctattgattt cccgacatgg tattggttac aacaatattg
    14161 atttaaaagc agcaaaagaa caccagacaa tcgtatctat catacctgct ttagttgaac
    14221 gaaatgctgt agcagaaaat aatataacta atttgttgtc catgcttcga cgcacgacac
    14281 aagcacaagc aagagtcaag gacgatcgat gggaagatcg ggcggagttc gtcggtagga
    14341 cgctgtttaa caaaacagtt ggtgtgattg gtgcaggaaa taccggaagc ggagtaatcg
    14401 aaattttgcg taacggtttt cgctgtgatg ttctagctta tgatccttac aaatcagatt
    14461 tatacatcga aagctttggt gcaaaaaaag tagaacttca agaattactg gaacaatcag
    14521 atatcatttg tttatgtgcg aatttgacag aagagaacta tcacatgatt tctgataaag
    14581 aagtcgatca gatgaaagaa aatgtttatc tatctaatag tgcaagaggc gctctagttg
    14641 atgaagaagc gatgattaat ggtctgaaaa gcggaaagat cgcaggatat gccacagatg
    14701 ttttagaaga agaaccggga cgtcaagatc atccgtattt agcgtttgaa caagtagtga
    14761 ttacgccaca tatatcggcc tacacaaggg aatgtttaga agcaatgggt gaaaaatgtg
    14821 tcagagatgt cgaacagatc agtcagcacc ttcttcctga acgttctgtc cagacaaaga
    14881 gcaactatat aaaaggatga tgaagaaaat ggtgaacggt ttaaaagtga aaacaggccc
    14941 tcaattttat ttatatgaag aaggtggtat ttcaaaagtt tctgatctct tgaaatcata
    15001 cggcgcgaaa agagtactgg tcacccacgg aacggtatca tgggaaaagg cgctacctaa
    15061 attagtattt ttgaatgacg aaacgattca atttttttat catcgttatt ctggagaatg
    15121 tagttatgct gaagccagac gaatagctac aatcatcaaa aaaatgaaat cgatttcgta
    15181 gttggcgttg gcggaggaaa actgaccgat ctagtgggtt atagtgccaa tcttgcaaga
    15241 atcgatttcg gagtcatccc tactttagct agtaattgtg ctccatggac acctcttgct
    15301 gtcatgtata aggagaatgg acaagcagaa gggaaaacag aacactttta cagacaagct
    15361 gcttttttta taacagatcc ccaattagtg attgattccc ctgttccata tttcattgcc
    15421 gggttagcag atacactagc aaaatggtat gaatctgagc tcatcttaga acaagatttt
    15481 ttgcaagatg aatcattttt gaagctagca caagatactg cgaagatctg caaagaagag
    15541 attttgaatc attctgctaa agcaatccaa gatatggcac aaagaaaact gacatcggag
    15601 ttcaaacagc tttcagagat tatctttgct gttgctggtt tagttggagg attaggagat
    15661 aaatacgcaa gaaatgcggc agcacatgct atgcatgacg ctatcagtaa atatattccg
    15721 gaaagccatc gttttctaca tggagaaaaa gtagcgtatg ggattttcta tcaactggca
    15781 ctagaaggtc gctgggaaaa aatagacgag ttatatcctt tgtatcggga attagaactg
    15841 ccggtttctc tagaacaaat gggattattt ccgaaagata aacagataat agataaaatg
    15901 gctgaattta ttgattcgaa agagaaagtg catttgattc ctatagaggt aacacagaaa
    15961 tcactgatac agtcaatgat ggaactggaa ggttatatca gaaagatctg aaggaggagg
    16021 acacatggaa acattaagtg tagtacaaag tttgttgatt gctttatggg tagccgcggt
    16081 tatgtcccgc tggttaggtg gaggagccac gttgacctta cgtttttctc ctttgatgac
    16141 tggattagtc gtcgggttga tcatggggga agttgcacaa gcgatgattg ttacagccgc
    16201 tttgcaaatg atttatatgg gggttttttc tccaggagga tcaatgccag ctgaaccatc
    16261 tattgcagca gctatcgcag taccagtcgc attattaggg gatctgtcgc cagaagcagc
    16321 gattgccgtt gcggtgccag tcggattgct cggaagttac ttgtatcagt ttcgtttttt
    16381 catcaatact tttttaggta aatatacaga caaagctgta gaagatttga atacaaaagc
    16441 tatggcccga tcaattattc tttatccaac ggttgcctca tttttattat tcgttccgct
    16501 tgttttcatt gctttatatt taggggcgcc attgatcgct gatgtaatca aagctttaga
    16561 gggaacagtc gttatccaca ttcttgaagt agtaggtgga gggttagcgg caatcggtat
    16621 cgcgacaact gtatacgtta tcggtcgaaa agattatctg gtctttttct tccttgctta
    16681 ttttatgagt gttgttttga aatctttaga tattacgatg gttacttatg ccatttttgg
    16741 gattattatc gctttgatct ttgtccagtc gcaaaaaggg aaacaagtac agcctgccac
    16801 aagcaataac aattcagcat cttttgatga cgaagatgac tatgatgacg gattttaatt
    16861 taggaggaat gggaaatggc tgaaataatg gataatcaaa caggtgctcc tgaagaaatc
    16921 acgaaaaaag atgtaacaaa agcctatctg agatggcatt ttgcaaacga aattcctcac
    16981 tcttttgaac gttacttggc gccatcacta ctatacggaa tgatgccaat cttgaaaaaa
    17041 ctttataaag atgaagactc tttaaaagca gcatataaac ggcagttact gttcttcaac
    17101 actcaattaa gctggggtgg cggtgtgatt actggcctaa tgtcttctat ggaacagcaa
    17161 agagcagaag aagagtatga agaaaaagag atcttgatgc aggatgactt gatgtataac
    17221 acgaaagcgg gattgatggg ggcactagca ggaatcggag atgcaatcga ttcaggaacc
    17281 gtccaatata tttttatcgc aatcgcggta ccttgggcac aacagggaag tcctttaggc
    17341 gcactttttc cttttatctg ctttgggttg tatcaggttt tactaggggt atttttcgca
    17401 agacaagctt tcactatggg ccgtaatgct acaggcctaa tgcaaaacac tggtgttcag
    17461 acagcgattg aaatgctatc agttttaggg ttgtttatga tgggggtgct tgcgggaaac
    17521 tatgtaaaag tagaatcttc attgaaattt gcgatatccg gacgtgagtt tgttatccag
    17581 gaaattcttg atcaaatcgt gccagggata ttacctttag ctgtcgtaat gggtgtgtac
    17641 ttgttctata cgaaaaaagg attgaaagtc acacaagcat tattatggct gactggaata
    17701 ttaattgtgc tagcaggaat cggtatttta taaaaagaag ggaacgtgaa taagatgggt
    17761 gtagtcaatt tagcgcgtgt ggatgagcgt ttgatccatg ggcaagtaat ggtaacgtta
    17821 tctcaaaaaa gtggagtgaa ttccattttt gttgtagacg aagttgtagc aaaagataaa
    17881 tttatgagag atctttacaa aaatgcagga agccgtacag gacaaaaaac aattgtcatt
    17941 acaccagaga aagctaaatt ctattgggat gaatatcaat ttaaagaata caattgtatt
    18001 ttgatcgcaa aaacagtcca aacgatttac gacttagtaa aacacggaat ccccatggaa
    18061 gaattgaata ttggagggat tgctcaaaaa aatcctgata cagatctttt agtgacgaaa
    18121 tctgtttatt taaataaatc agatgctgaa aaattgaaag aaatgaatga acaatatggc
    18181 gtaaagaata tttattttca agccactcca tctgcaccta aaacaggatt aaaagatgta
    18241 ttgaacaagt ttgatttgtc ataaacaaat gcttagaaaa aagataaaga gggcgaaaaa
    18301 atgaagaaga acgagctgtt tcgtgattgg gaatttcgtt atcgctatat ttaccgtaag
    18361 cgccgtacaa aaaagagtaa acagcgtttt ctttcagcat tagtatcaga tatttacagt
    18421 atgagaacag atgtgacagt cattgcttac gatacgctag cctaccgatc aaaaaatatt
    18481 tatgttggag atatcgaaaa agcagagaaa gttatctgta cttattacga tacacccgtt
    18541 catgcattag gaagttattt catgtttgat tggaaagacc aaaggaaaaa aacaatctac
    18601 tctatattat tgtccttcat tttgttgttc agtttaggtt ggtggggaat gatgatatat
    18661 aacaaaaatc ctcatcacgt attcgatctg ctatccgtat aaacaggtat cactgtacta
    18721 gcatttggga gctacttttt cttattggga aaagcagctc gcggatggcc tagcagacaa
    18781 acttttattc gaaatacttc atccatatta acgatgctag aaatgatccg tacgattgat
    18841 gatccgagtg tagcgtatgc gtttgttgat gaaggctgtt atggagaaaa gggcttagac
    18901 agcgtgagat cgggtatgaa aaaagaggct attctttttt atctagactc tgttggggca
    18961 gatacaccgc tacagttttc gggaaattat ttttcaaata aagaacagtg gctgaaacaa
    19021 gttgataagt tgaaagaaaa aaacgtcaat tatattttta gtgcaagaaa aaagcaagcg
    19081 cagttcttct atttgaccaa aacagattta cgagggaaaa catttaattg gcaaaatgcc
    19141 aatcagatta ttgctctttt tcgataagta aaaagtgcaa aacaaggagt gagaagacta
    19201 atgttggaaa tcatgatcgt tacacatggt aagttgagcg aaggactgaa agatgcggcg
    19261 gaagtaattg tcgggacatc gagccatatt gaaacaatgt gcttagagtc tggggaggac
    19321 atccaaacat tcggagagaa ggtcaaagaa aaattaacta tgatcaacac agcagaaccc
    19381 atgctagtat ttgttgatct aattagcgca agtccgtaca atcaagcgct cttggcagtc
    19441 aatgaactga ctgaagaaaa gaaagcagcc acttatgtca tagggggcgt gaatctgcca
    19501 atgttgttag aagcaatcaa tcatcagatg cttcacacac cgatagaaga agcggtggaa
    19561 caaattgtca aacaaggaaa agataattta actgtttggc atgcttcagc ggaagaaaaa
    19621 aaattagatg aggacgattt ttagaaggaa ggctgaaaag atgaagtggg gatttagatg
    19681 gtatggtgaa aaaggggatt cgatcccatt aaaatatgtt cgccaaatac cgggaataaa
    19741 tgggataatt ggaactttgc tgactaaaat gcctggagat gtttgggaga tttcagaaat
    19801 tgaggagttg aaagcatctg ttgagagcca aggactggaa ctgcttggaa ttgaaagtgt
    19861 agcgatccac gatgcaatca aggcagggac agatgaacga gattactata ttgataacta
    19921 tatacgaaca atcaaaaact tgtctcgatg tggggttcga ttgatttgtt atagcttcaa
    19981 accaattttt ggatgggcga aaactgattt gtttaatgaa aacaacgatg gaagctattc
    20041 tcttttatac gatcagaaaa tcgtcgatca gatggagcca ggagaaatgt atcaattgat
    20101 acacagccag tcaaagggat tcaaattgcc gggttgggaa gaagaacggt tgaaaaaatt
    20161 cgctcagctg gaaaaactgt atgaaggaat gaccgaagaa gaattatttg aaaatctgac
    20221 ttatttttta gaaaaagtga ttccagtgtg cgaaaaagca gatgtaaaaa tggctatcca
    20281 tccagatgat ccgccatggg aactttttgg ttttcctaga gtgacgaaaa atcttggcga
    20341 cttgaagaaa attctaaatg cagtagattc tccatataat ggactaacgc tgtgcacagg
    20401 atctttaggg gcaaatccgg aaaacgattt agttgaaatc atccatgagg taggttcgcg
    20461 catccatttt gtccacttca gaaacgtgga gtttttagga gaaagaaagt tcaaagaaac
    20521 ggcacatccc tctacagaag gctcccttga tatgtatcat attatgaaag cgttggtgca
    20581 agaagggttc gacggtccta tacgccctga tcatggtaga accgtgtggg gtgaagtggc
    20641 tatgcctgga tatggcctgt atgatcgtgc aatgggactc acttacatgc aaggtctata
    20701 tgaggcgata agtaaggaaa aaccactggg agagcattat gaataaaatc aagctcttca
    20761 tctttgatat ggatggcctt ttattagaaa ctggacgttt agcttatcgt gcttatgtaa
    20821 aatcagcaca aaagtatgat tatgaaatgc gtaaggaagt ctattattta ctaactggcc
    20881 aaacagaaat ggcgataaga gaacaaatgg gcattttgta cggagaagat gttccatata
    20941 tcaaatggcg agaagcaatc aatcaatata aagaaaaaat cgtgaaagaa gataagagag
    21001 tatatacgaa aaaaggagca gaagaaattt tatcttttgc aaaagaaaga ggaatccata
    21061 caatcgtggc ttcgtctaat acacgggtaa aagtagaaat gtatttaaaa atggaaaatc
    21121 tatatgcctt gtttgatcaa atcatttcag gggatgatgt aaaaaaagga aagccggaac
    21181 cagagatctt tttgaaagct tgttcaaaaa tgaatattcc tccaagtgaa gcacttgtat
    21241 ttgaagactc gattgcaggg atcgaagcag caaggcgagc gggcattttg tcttttctca
    21301 tcaaagatca tttgataggg ttgcccgatc ataatgggaa gcataagttg aaagtagatg
    21361 taagtttata tgaatccaat aaagcagact atgaatttta tgacctgcat caagcaaaac
    21421 gatttttaga aagtaaaggg ttgtatctct aataaagagg actattttct agtcctcttt
    21481 atttattgtc ctgcacgact gagggaacag atgaaaaaga agtaattttt tattttgaag
    21541 tatctacctt atttgtagtg aacaatttag aacaaacaca aaacgcatct actgttatct
    21601 accagtagat gcgtttaaag agaagaacga gaatcgtcca tatgagggtt tccactcctt
    21661 atgcagattt ttttaattct tgcactgctt gttcgatcgt aacacctgtc gctacatgat
    21721 tggtattttc tttgtcaaaa acgacaaata gattcaaatc tgtatccaag gttactcgat
    21781 attgtatatc tttgttatag aatgtcatag ccattctcct ttctttaaat acttgctgaa
    21841 aggcaacgaa cgcgttgata acgaaattcc atttataagt ataccaccaa ttgaaaggtt
    21901 ttcatagtta agaaatttta aagtcaaaag ttgcagataa aagcaaataa acaagaaaac
    21961 aaaaagtgaa tcaatcgcgg ttattcaact ttttcttcga ttcgaagaca atagaagaca
    22021 atgaatttta tatttcattg cattatcctt gtgttattag aaaaagcatg atatattaaa
    22081 tgagtaatct attttgaaac gtaatttgag cgatatatct acatagataa aactcctttt
    22141 gcctatcaaa tatttattgc aacgaaaaaa cgtacattga tacgtatcag gaggaattta
    22201 tttatgaata acggtacagt aaaatggttc aacgcagaca aaggttttgg ctttatcact
    22261 ggtgaagatg gaaatgatgt atttgcacat ttttcagcta tccaaggtga cggcttcaaa
    22321 actttagaag aaggccaagc agtgtcttac gatgttgaag atggtcaacg tggccctcaa
    22381 gcaacaaaca ttgttaaagc ttaataaaaa ttgactttta accgtctttt cgaaagaaaa
//
LOCUS       DAZABD010000046        21626 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000046 DAZABD010000000
VERSION     DAZABD010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21626
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.046"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(588..1613)
                     /locus_tag="ACHALG_001864"
     CDS             complement(588..1613)
                     /locus_tag="ACHALG_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN2587248.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            1888..3573
                     /locus_tag="ACHALG_001865"
     CDS             1888..3573
                     /locus_tag="ACHALG_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFN2587249.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            3587..4870
                     /locus_tag="ACHALG_001866"
     CDS             3587..4870
                     /locus_tag="ACHALG_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN2587250.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            4940..5809
                     /locus_tag="ACHALG_001867"
     CDS             4940..5809
                     /locus_tag="ACHALG_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN2587251.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            5806..6642
                     /locus_tag="ACHALG_001868"
     CDS             5806..6642
                     /locus_tag="ACHALG_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN2587252.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            6668..7735
                     /locus_tag="ACHALG_001869"
     CDS             6668..7735
                     /locus_tag="ACHALG_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFN2587253.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            7732..8559
                     /locus_tag="ACHALG_001870"
     CDS             7732..8559
                     /locus_tag="ACHALG_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFN2587254.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            8604..9629
                     /locus_tag="ACHALG_001871"
     CDS             8604..9629
                     /locus_tag="ACHALG_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFN2587255.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            9626..11809
                     /locus_tag="ACHALG_001872"
     CDS             9626..11809
                     /locus_tag="ACHALG_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HFN2587256.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            11809..12939
                     /locus_tag="ACHALG_001873"
     CDS             11809..12939
                     /locus_tag="ACHALG_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFN2587257.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            12936..13613
                     /gene="pgmB"
                     /locus_tag="ACHALG_001874"
     CDS             12936..13613
                     /gene="pgmB"
                     /locus_tag="ACHALG_001874"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFN2587258.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            13697..14224
                     /gene="msrA"
                     /locus_tag="ACHALG_001875"
     CDS             13697..14224
                     /gene="msrA"
                     /locus_tag="ACHALG_001875"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFN2587259.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(14425..15111)
                     /locus_tag="ACHALG_001876"
     CDS             complement(14425..15111)
                     /locus_tag="ACHALG_001876"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFN2587260.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            15191..16729
                     /locus_tag="ACHALG_001877"
     CDS             15191..16729
                     /locus_tag="ACHALG_001877"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFN2587261.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            16849..17316
                     /locus_tag="ACHALG_001878"
     CDS             16849..17316
                     /locus_tag="ACHALG_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFN2587262.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            17850..18278
                     /gene="rplM"
                     /locus_tag="ACHALG_001879"
     CDS             17850..18278
                     /gene="rplM"
                     /locus_tag="ACHALG_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFN2587263.1"
                     /translation="MAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPTFTPHL
                     DTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLIETSVK
                     GMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            18294..18686
                     /gene="rpsI"
                     /locus_tag="ACHALG_001880"
     CDS             18294..18686
                     /gene="rpsI"
                     /locus_tag="ACHALG_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFN2587264.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(18774..20000)
                     /locus_tag="ACHALG_001881"
     CDS             complement(18774..20000)
                     /locus_tag="ACHALG_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN2587265.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(20072..20374)
                     /locus_tag="ACHALG_001882"
     CDS             complement(20072..20374)
                     /locus_tag="ACHALG_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003013637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN2587266.1"
                     /translation="MTIAANNDTSVRLAVNWGEVKFLCAYPKQFKNFITTILGDDFIE
                     SRVYLNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKDFMNTFKIT"
     gene            20922..>21626
                     /locus_tag="ACHALG_001883"
     CDS             20922..>21626
                     /locus_tag="ACHALG_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFN2587267.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFI"
BASE COUNT         7034 a         3827 c         4476 g         6289 t
ORIGIN      
        1 aaaaaagagg ttgtgacaaa ttattgtcac aacctctttt ttggtttgtc taaaataatt
       61 tgtctaacta gaagaaaagt gggaaaggat cttaacaaca tcggttcgta tccaattaag
      121 gtatcggaac gatcatcttc caagagaagt caatcaacaa aagtaaagat agatcatttt
      181 ttgaatgcaa aataaattga tgggaagata gagcggttat ttttaatatt ttgctagcta
      241 ttattgaata attcctcgaa taaaacaaaa ccagtagaca tgtctgttat tctatttatg
      301 ttttagagat aagtatcccc aaataacatg gattgaaata agggtaaatg tgtttaaaaa
      361 aacgaactat aaaaacttat acacaacgtg gttaaaaata gttttttctt ccctgtggaa
      421 aaaatagttt aacacaaaca ttatagttat ccacagctat ctaaaacaga tggaaaataa
      481 gaaaaattcg ctaaaatacc gggagggggt aacgagggat gagtgattga aaaaagaaca
      541 gagtaactga cttatcctca gtgtttctgt tcttttataa ttctgttcta catttcttgt
      601 tgttggatcg aatctgtgag agtaatctcg gttgaaagaa tgattctttt ttcttcaggg
      661 tattggtggt ggattagttg gaataaaagt tctgtggctt tgattcctaa ctgatttgtc
      721 ggaatgtcta tccgagtcaa ctgtggcgaa aatagggagg acaataaggg atcactgaat
      781 cctgcgatcg aaagatcatc aggtatctgc tttccagatt gttctgccac tttgtaaata
      841 ctcaatgatt ttaatgcatc ggtagaaaaa atcgcagttg gtctttctcg tagcttcatt
      901 aaatcattca ttgctgccat gctatcttct aagcgaaagc cgccgtctat aatccattca
      961 ttacggatat ctaggtggtg atcaaataag cattgacggt aaccattgag acgatcattg
     1021 gatacataaa cttctgtcgg agcatggaga cagccgatat gacggtgtcc attcttgatc
     1081 agtgtttcca ccaaatcgta actatcctgg aaattatccg tgtccactga gtagatgttc
     1141 gtgtagtcgt ttccgacttt tccgattacc acaataggaa tattgtattg atccaattca
     1201 aaaagaagtg tttcatccat cgtagagctt aaaataataa ttcctcggat cattttttct
     1261 tggatctttt tcgcgcattt ttctagctct tcttctggtg tttttgccgt ttgtagaatc
     1321 aagtcgaagt tattttgttc agaaacggca gaaatggatt gaataacatc tgaaaaaaaa
     1381 ggattgccat aggtcgtatg agaagtacga tgagataatg ctagaatcgc gtcaaaacca
     1441 tttcctgaca gtcctcttgc aattttgctc ggttcataat tcaattcttt gattgcttga
     1501 agtacacttg cttttgcttt atcagagata tttggctggt tatttaatac tctggagaca
     1561 gttgattttg agacattagc ttttttcgca atgtcataaa tagtaggttt caattttata
     1621 tgcacctcct ttttgggagc gttttagaat aattccatag tacaatagac attctttttt
     1681 gtctatgatt ttgaaaattt aggaatagct tgcatgatca tgagagacca tattttttta
     1741 agaagctatt aattattgat acataagctt tcttatttgt atttttgtgt atatcaaaat
     1801 tatttaaaaa aatgctattg acagtaagcg ttttccataa tatgatcaaa tcgttgggaa
     1861 cgctcccaaa aaagaaaggg gaatccgatg tatacaaaac ttttacaagc gatttatcgc
     1921 gactcagatt tgtctgagaa aaccgcctct atgattgaac aaaaagtcag tgtttatcag
     1981 cagaagaaag gccaggcagg caaactgcat tgggacgaag aagatatttt cttgattact
     2041 tatggggatc aattttttga agagcagcaa ccaactttgc gtacatttaa gaaattttat
     2101 caaaagtatt tggcagatac atttagagtt gtacatttct tgccattttt cccatataca
     2161 tctgatgatg gtttctccgt tgtaaattat caggcaatca acgaccgttt gggcacatgg
     2221 gaagatatag aagaaatgag aagcgaaaca cggttaatgt tcgactttgt ctgcaatcat
     2281 atgtctgccg aaagtacttg gtttcaggaa tatttaaact tgaatgatac gtacaaagat
     2341 ttttttattg atttgccact ggatacagat acaagcagcg tgactagacc aaggaccagc
     2401 ccgttactta caacgttcat tgacaaaaac gggaaagaaa aacacgtatg gacgactttt
     2461 agcgaagatc aagtagactt gaattttaaa aatccagaag ttttattgaa aatgatcgat
     2521 attcttttgt tttacttaga aaaaggcgct tcatttattc gcttggacgc tgtgggattt
     2581 ttatggaaag aactaggaac ttcttgtata cacctgccag aaacgcataa aatcatccaa
     2641 ttattccgtg caatcgctga tgaagcagct ccaggaacgg tattgatcac tgagacaaat
     2701 gtgccacaca aagataatat cagttatttt gggaatggcg agaatgaagc acagatggtc
     2761 tatcagtttt cgctaccacc tttgaccctt caagcgattc gttcgggtaa tacaagtaag
     2821 ttgaaagaat ggttaaaaac catcgatttc gcatcgaaaa aaacaacttt ttttaatttt
     2881 ttagcctccc atgacggtat cggattgaat ccggtacgag gtattctgcc ggaaaaaacg
     2941 atccttgaat tagtggaaca gctgaagaaa gagggtgccc gagtaaacta caagaaaaac
     3001 ccagatggaa cgaccagtcc atatgagata aacgctacgt atctagatgc gttaagttct
     3061 ccaacagata cgacggaatg gagaagcaaa cgctttttag ctgctcattc atttttggta
     3121 acgttaccag gcgtgccggc aatttatatt caaagtgtgc tgggaagtcg aaatgattac
     3181 aaaggcgtag aaaaaacagg agagaatcga aaaatcaatc ggcaaaaatt tttgttagca
     3241 gatattgaga aagagttgca gacagatcca ttaagaacag cagtatatgg tgaattgacc
     3301 cagatgctat ctattcgaaa aaaagaaaaa gccttccatc cgaatagttt gatttactta
     3361 ctggactcag cagattcttt tatcagtttt attcgcgaag cgaaggaaga gaaagtctta
     3421 gttatccata atttttccaa tcaatcagca ggttatcttt taccaaaagg agattaccta
     3481 gacctgtttt ctggacaaat tatccattgc agtcaagaac gaaagttgat attccagccg
     3541 tatgaatata tgtggctgaa aataataaaa taagaggtgg aaaaaaatga aaaaaattat
     3601 tgttactacc tgtctgctag ctactggatt tcttcttact gcttgtggtg aaagtcaaag
     3661 ctctaacgaa tcacaacaga caacgattca attcatgcac tcttctgtcg aacaggaacg
     3721 attaagtgtg atcaatgaac tggttgctga ttttgaaaaa gaaaatccag acatcaaaat
     3781 caagcaagtg ccggtagaag aatcagcgtt caatacaaaa atcgttacgt tagctcgttc
     3841 cggtaaatta ccagaagtaa tggaagtcag tcaggatttt gctaaagtga tggataaaga
     3901 tgaactgatt gatcagacgg cagtccaaaa cgttatcgag gaagctggag aagataatta
     3961 ttatgacgga gccaaaaacc ttgttcgttc agaagatgga aaaagctata ttgctgcacc
     4021 aattagcggc tgggttcaag gtatttggta tgacaaagaa aaattatcag aagctggttt
     4081 ttcagaacca gaaaattggg atgatatttt aaaaattgct caacatttca cagataaaga
     4141 gaataaacaa tatggaattg ctatgcctac tgctgaagga acaatgagtg agcaagcgtt
     4201 ctctcaattt gctttgtcaa atggagcaaa tgttttggat gatgaaggcg aggtaacgat
     4261 cgatacggaa aagatgagag aagcattgtc gttttatcaa aacttatccc aatacacgat
     4321 gccaggctct aatgatgtga cagaaatcaa agatgctttc atgaatggaa cagcaccaat
     4381 ggcaatctat tcaacttaca ttttgccttc tgtttatgaa gcggggaact cagaaaatat
     4441 cggttttgct atcccaacac aaaaagatca agctgtctat ggcacagtat ctggattgac
     4501 gatttcttct ggtctagacg agaaacaaaa agaggctgct gaagcattca cagctttctt
     4561 atctcaaccg aaaaacatgg aaaagtgggt cttgatgtct cctggtggtg ctcaaccagt
     4621 gaataaacaa gtagtcgaac tggatggtta caaagaaaat gaagtcatca agtcttttgg
     4681 cgagctgcca tctgagattg ctagtgcttt tgatgaagta caagtctttg gactagtcgg
     4741 ggaaaagaat ttcacgaaaa tgggagatat cacaagctca ggtgtgatcg gaaaagcagt
     4801 gaaccaagta actgtcggaa acgaagatgt cgatcaagct ttagctgacg cacaaaaaaa
     4861 tacgaaataa caagaagttc tgccaatggt gttcgaaaaa agtagatagc tattggcaga
     4921 ttatcagggg gaggtttcaa tgaatcgtcc aaggaaacat tctgacttaa aattagccat
     4981 gggactttta gcaccaagca tgcttttatt agctgtttta gtgctgattc caatgctttc
     5041 caatattcag atcagttttt tcgatatgcc gctgaatcct aactcagacg ccgtttttat
     5101 cggattaagc aattatcttg aaattttaag tgatagtgaa ttttatcgtt ctttaggttt
     5161 aacagttgct tacacattct tagttgtagc aggcagtaca gctttaggat tagccgtagc
     5221 tattttgctt aaccgcaagt tcagatttag gaaaacggtg cgttcactga ttttattaag
     5281 ttatgttacg ccatctattt ctttgatttt tgcttggaaa tatatgttca ataatagtta
     5341 tggaatcgtc aatgatctgt tagtagatag gttgcagctt ttcaacgaag caccgctgtg
     5401 gttcgaccag ccagttgcta gcttctttat ggtcgttttg tttgctatct ggagatacta
     5461 tccgtatgcg tttatttcat ttttggctat tttgcaaaca gtcgacaaaa cgctttatga
     5521 agcagctgaa atggacggtg caaatgcttg gcagaagttc aagatcgtga cactgccggc
     5581 aatcaaacca gtactagcaa ctgtgattgt tttacgatct atctggatgt tctatatgtt
     5641 tacagatgtc tatctactga cgaataaagt caatatctta ggcgtttatt tgtatcagac
     5701 agcctttgcg ttcaatgatc ttggaaaagc agcagctata tcgattctat tgttcatcat
     5761 tattgcagcg gtcgtcttgc tctcacgaaa gaaggtggac ttgaaatgaa aaataaaaag
     5821 agcaggaaaa tcttatttta cgcgacgatt ttgttgctat tagctgtcaa tttgtttcca
     5881 tttttgatca tgctgatgac atcattcaag gattcacaag aagcaattgc aacgacacaa
     5941 actatttttc cgaaagaatg gacttttgaa cattatcagg atattttcaa tgctgagatt
     6001 tttccattca tcatgtattt caaaaatagt ttagtgatct ccttgatagc ctccgtaact
     6061 gcagtagtga ttgggatcag cggggcatac gctttgtcaa aactacgctt caaagctcgc
     6121 tcgaaaatca atgctagctt ttacaccgtt tatatgtttt ccggtatttt gatcgttgtt
     6181 ccattattta aaatcatttc cggtctaggt ttgtatgata ccaatacagc actgattatc
     6241 acgatggtcg ttcaaacact tcctactgcg atattcatgc taaaaagtta ttttgacacg
     6301 attccgactg atattgaaga agcagcaatg atcgatggtc ttagccggat ccagatcatt
     6361 attcggatca ttataccatt ggctatttct ggaatcattt ctgtcttcgt ttattgcttt
     6421 atggtttcgt ggaatgatta tttatttgct tcgattttcc tgtcgacctc cgaaaaattt
     6481 actttaccaa tcggtttgaa cacattattc agtacaccag actacatttg gggacgaatg
     6541 atggccgctt cattggtgac cgctttacca gtcatcatta tgtatgcgtt atctgaacga
     6601 tttattaaag gtgatttagt cgaaggcggc gtaaaaggat aaaaagaaaa gaaagaggaa
     6661 agaaaagatg aaaaaattag ttgcattaga accaagaata gccggattca aagattacga
     6721 agaccgtgag atcgaaaaga acgaagtacg tatccgagta attttcgcta ctccaaaacc
     6781 aggaacagaa attgtcgact ttagaggatt aagtccattt atcgatgaag aattttcaga
     6841 agaatggcgc ctattcttgc caagagaaga aggaagccaa cgcggtgtgg tatttggcga
     6901 atttcaatta ggaaatatga ttgtcggtac gatcgaagaa acgggcagtt tggtgacaga
     6961 gtatcaaaaa ggagagttag tctgcactta tggacccatt cgtgaaacgc tgattacaaa
     7021 cggtgttgat aattataaac tacgtaaact tgatcggttg gatcaatgga aaaatgcagt
     7081 atgttacgat cctgctcaat tcgctttagg tggtatccgg gaagcacatg ttcgagcagg
     7141 tgatgctgta gcaatcttcg gtttaggcgc tatcgggcaa attgcgattc aattagctaa
     7201 aaaagcaggt gctactacca tcgttgggat cgatcccata gcgcagcgta gagaattagc
     7261 agaaaaacac ggtgccgatt acacgctaga tcctatgaaa gcagatgtcg gttttcgtct
     7321 aaaggaattg acagataaac gtggagtaga ttcagttatt gaaacgagtg gcagtgctgc
     7381 cgcattacaa gcttctttaa tagggatcgg ctatggcggg atcatttctt atgttgcctt
     7441 tgctaaacct tttccagctg ggtttaatct tggtcgcgaa gcacatttca ataatgcaaa
     7501 aatcgtcttt gctcgagcat ctagtgaacc aaacccagat tacccaagat ggaaccgtaa
     7561 acgaatcgag gacacatgtt gggaattatt gatgaacggt tacttggatt gtgaagaaat
     7621 cattgatcca gttgtgccat ttgcagaaag cgcagaagct tacatgcgtt atgtagacca
     7681 gcacccagaa gaaagtatca aaatgggcgt tcaagtccga ggaggagaac aatgaaattc
     7741 ggtacgcaag atcaagcatt tttcccaaga gacattttag aaaaatttag atttatcaaa
     7801 gactgtggat ttgaagaata cgaaatcgac ggtaagttgt taatagaaaa tatcgacaca
     7861 gtcaaacagg caatcaaaga aacagggatc ccagtagtga cagcttgtaa cggctacgat
     7921 gggtggatcg gtgactttat cgaagaacgg cgtcttaatg gtgtacgtca aattgctgag
     7981 attttgcgtg cactaagcga ggttggaggc aaaggaatcg ttatcccagc agcttgggga
     8041 atgttcactt atcgattacc gccgatggtt tcaccccgaa gcaaagaagg agactataaa
     8101 gcagtcagcg aatcacttcg ttatttagat aaagtagccg aagaaacagg tacggttatt
     8161 tatttggagc ctttgaaccg ataccaagac catatgatca atttgttagc ggatgcgcgt
     8221 cattacattg actctaatca attgaaacat gtgcagatca tcgctgattt ttatcatatg
     8281 aatattgaag aagatgatat ttcaaaagca ttgactacgc aaaaagagta tgtgggtcat
     8341 atccatttag cagataatca tcgctaccaa ccaggatctg gcgcaattga ttttactcgc
     8401 cattttcaaa cgctacttca aaatgagtat gaaggttata tggtatatga atgtcgtttg
     8461 cgaggagaaa atgaagtgaa aatttatcag gaatcattgc aatacttgaa aaactgtctg
     8521 gaaacagcgc gtcatttaaa tgatttagag aaagaataga gaaagaataa caataagtaa
     8581 ataagcaaag gagagagaaa taaatgaaga aagtagcaat cataggcgct ggacaagtag
     8641 cagaaaaagt acatgctgct tattatcaaa aaagatcaga tgtcttagaa ttggccgctg
     8701 tggttgatcc agtcagccag cgagcagaag acttttgccg acgtacagga ttttctcatg
     8761 cgtatacatc agttacacaa atgctgaatg agatttctcc cgatatcgtg agtgtctgta
     8821 cacccaatcg ctttcacttc gaacatgtat tggctgccct cgaagctaat gcttcagtga
     8881 tgtgcgagaa accaccagct atgacagctg atcaagcaag aaagatggca gagacagccg
     8941 ataaaaacgg gcagattttg gcttatggat tccagcatcg tttttctgac gaagcaacaa
     9001 tgatcaagaa tcatcttgat gttctcgggg atatttatta cgtagaagcg aatgcgcttc
     9061 gtcggtctgg tgttccaggc tggggaacgt ttatcgacaa agaacttcaa ggaggcgggc
     9121 ctttgatcga ttatggcatt cacatgcttg atacagcgat gtatattact ggatttccta
     9181 aagtaaaaag cgttcgggcg tacagcttcc aaaaaattgg tacgaaaaaa aattcaggga
     9241 aattcgggaa atgggatcca agcaaattca cagtagaaga tgcaattttt ggcacactgg
     9301 aattagaaaa cggcaagatc atccaactga atacttcgtt tgctttgaat atcagagaag
     9361 acaaactgaa tgtggctttt tgtggcgatc aggcaggtgc taatctgtat ccaggcgaac
     9421 tattcgaaga tgtagatgga aagctggttt ccttacaaaa gaccacgcca gttaatgacc
     9481 cacatgaact aagtatggct cattttgtga atcgggcgct cggtgaccag actcagatga
     9541 ttgcagatgg ctggcaagga tatgtcatcc agaatctggt ggaaaattta tatcgttcgg
     9601 cagaaacagg agaagttatt accttatgaa agtagaagaa aaacagtttg atttagagta
     9661 tatcaataag tatgcgagtc agatgaccat tggaaatgga ttttttggta tcagaggaag
     9721 ccaggaggaa gactatcgga atcaagtaag gggaatgttt gcagctggtg tatataaccg
     9781 acctttagga gcagaatcag ccgagttagt gaatctccca gatgtcatca gatgggaaat
     9841 aaaaatcgac ggtgaacatt tttctcttga gacgatgaaa gttcttgcat acgagcgggc
     9901 actagattta gaaatgggag agcttaaacg ctatgcgctc attgaaactg ctcaaggaaa
     9961 aaaagtggaa gtcattactt gtcgtgttgc tgatcaaaat aatctccatc aggcagccct
    10021 gaaagtggtc atcaagccgt tgaccgaaga ctgccaagta gatgtgaaaa cgggtatcga
    10081 tggacagcaa accaattttg gtacacagca gttggttgaa aaagaactgc gagtatttgg
    10141 tgacgatttg atcagtgcat cctaccagac aacagaaagt aaaattgata ttgggattgc
    10201 agctaaattc aataaagcag gtgtattcca agcaaaaaat cgccggatca ccgcttcctt
    10261 ttcagaaaag atcagaaaaa atacgtcctt tacgctggag aagatcatcg tgctctctac
    10321 ctctcttcag caaacagacc cgtttaagaa aagtattgcc caagcccaga aaagcgttag
    10381 ttatacagaa attttagagc agtcgatgtc ttattggaca gatttttggc atcagcatcg
    10441 gataacgatc caaggcgatt cttctttcga tcagttagcg atggacttcg ctgcttacca
    10501 tttacagatt atgactcccc gcaatgatcc aagtgttagt gtaggggcga agggactaac
    10561 tggcgaaggt tataaagggc atgttttctg ggatacggaa atttttatcc tgcctttctt
    10621 tttatatcag tttcctgaag tagcaaagaa tttgctgctg taccgttatg atcgattaga
    10681 aggcgcacgg caaaaagcaa gagattatca ctatcaaggt gcacttttcc catgggaaag
    10741 cgcacacact ggtcaggaag agacacccaa atttgctgca ttgaatatca aaaccggaac
    10801 aagacaagaa gttgcttcag ctgccgcaga acatcatatc agtgcagacc ttgcttatgc
    10861 cgttcaagag tatgtccaag caacagagga tcaatcgttt ttagaaacaa agggaaaaac
    10921 gctcattcat gaaacagcag ctttttggct gagcagagct gtaaaagtcg atgatcattt
    10981 agagatacac agagtcattg gtccagatga atacacagaa tatatcgaca ataatgcata
    11041 tacgaactat ctggcgcatt ataatgtggc cttagctgtt aaatatcaag ctggcgatcc
    11101 gctttttttg aaagaatgtc aaaatttttt agataagtta tatttgccag tgcctaacaa
    11161 agaacagctg attccgcaag atgacacgta tctcagtaag ccagttattg atttgaccac
    11221 ttacaaagaa cgtcctggca gccagtctat tttacttgat tatgcccgta gcgaagtaaa
    11281 tgaactccaa gtcagcaagc aagcagatct tgtcatgctg ttatacttat tacctgagta
    11341 tttttctcaa ccaatcgttt ctaagaattt gcattactat gaaaacaaaa caatccacga
    11401 ctcatcgttg agtaaagcga ttcacgccat agaggcttgc cgtatccaag aaatggaatg
    11461 ggcgtatcat ttattccaag aagcgtgcct gattgattta ggaccagatc cgcattcatc
    11521 agatgacgga attcatgctg cttcattagg agcactatgg ctagcagtta tatttggttt
    11581 ttcaggtatt acagtaacga atggaactct ttctatttct ccaagacttc cttcagcatg
    11641 ggaaaaaatt tcttttcctt ttatttggaa aaatcacagg ttgctatttg ttttgacaaa
    11701 aaaacaaata gtaataaaaa aagagacgaa agaaacatta cctatttttg tcaatggaga
    11761 ccaataccag ctggaaaaag aactgatctt ggaacggagg ggaaaataat gcgtattctg
    11821 attgcgccag attctttcaa agaaagtctg acagccttag aggcagcaca agccattcaa
    11881 tcaggttttc aagaagtttt tgttcaggct gaatatgatc tgctacctat aggcgatgga
    11941 ggagaaggaa cggtttcatc attagcagac agtttaaggt tgaagcggca aacagcagtc
    12001 gttactaatg catttggcaa aaacaaagaa atccagtacg cttcagacgg tcaaactgcg
    12061 atttttgaaa tggcagaaat cgtcggattg aaagatgtcc cactaaacca acaacagcca
    12121 cttaggttat ccacgcgagg agtaggagaa ctgatacgac aattgttgca acaaggtgtt
    12181 cgacacatca tcatcggcgt tggcggaagt agtaccaacg atggcggcat cggcatggca
    12241 gaaggtctag gttatgcttt ttacgatcaa gataatcaaa aagtagaagc actgggagaa
    12301 aatctaggta aaattgctac agttgataaa acgaacgtgc ctattgagct gattaaagcc
    12361 aaaatcactg tcattactga tgttactaat cttttatgcg gaaaagaagg cgcttctttt
    12421 gttttcggac cacaaaaagg cttgaaagaa aatgaattag cagtggctga ccaagccatg
    12481 catgattttt atatgagatt tgcccctcaa gtaatcgagg catcgggcag tagtgctggt
    12541 ggtggaatgg gtgcaggact gttagcattt gcttcaggga aaatagtctc tggcatcgac
    12601 tacgtattgg atttactgga gttcgataaa cgggtacagc aagcagacct cgtaatcgta
    12661 ggcgaaggac gtctagatgg acaaagtcta aaaggaaaag cgccagttgg gattgctaga
    12721 agagtaccag agggcgttcc tgtgatagct atttgcggaa gtgttggtaa aggaagcgaa
    12781 aaagcagcag atgttggcat ttcggccgtt tttccgatcc ttcctgcagc tgtgccttta
    12841 caagaagctt tacagcaagc cactgataat cttcatcgca cagctaaaaa catcgcatct
    12901 ttacttcaag tcgttcattt tcaaggaggt aatcgatgaa aatcaaagca gcactattcg
    12961 atttagatgg cgttcttgta gacacagctc gttatcatta cgaagcgtgg ctggttttag
    13021 ctaaccagct gtccattcca ttcactgaga aagaaaacga gcaactgaaa ggaatatcca
    13081 gaaccgagtc actagagcga ttgctgtcat ttggaaaaat ggaacagaaa ttctcagaaa
    13141 aagagaaatc agcctttgct gagcaaaaaa acaatctata tcttcaagcg attcaaaaaa
    13201 tggatgaaac atccgttttg cctggtgcaa tagctgttct tgagtacttg aaaaaaacaa
    13261 acatcaagat cggattggga tcagcaagca aaaatgcacg cctcatttta gaaaaaacga
    13321 atttgacaag ttattttgat gtccttattg acggcacgca agtatcaaaa gcaaaacctg
    13381 atccagaagt ttttctcaaa ggagcacagc aattgaatgt accgccaaat gcatgcttgg
    13441 tcattgaaga ttcagaagct ggttgtcaag cagctttggc tggtaacatg catgtattgg
    13501 gaataggtga aaacataaat cttccttctg ctgaatatgt gattcctgat ttaaccgttt
    13561 ttgatcaagt acgttcgttt tggcatttat cagaagccgt atcacgtata taatagtttc
    13621 cgttttttct gtcacaagca aaacgttatc ctccttttgg aaatcaaggt atgatgagaa
    13681 aaaaggggga ttttatatga caaaaaaagc agttttcgca ggcggatgct tttggtgtat
    13741 ggtcaaacca tttgaagagc agccagggat catttctgtg acatccggtt atactggcgg
    13801 gcatgtacca aatccaactt atgaacaagt aacaacaggc acaacagggc atacagaagc
    13861 agtagagatc gaatatgatc cagaagttat ttcttatgat cagttggtgg aaatctattg
    13921 gcagcaaacc gatccgactg acgcattagg acagttcgct gatcgcggtg attcttatcg
    13981 tccagtgatc tactataatg atgaagaaga acgaaatatt gcagagcgtt caaaacaagc
    14041 attgcaagac agcgggcgat tcgataagcc aatcgtgaca aaaatcgaac caagaagcga
    14101 attttatcct gcagaagatt accatcagga ttattacaag aaaaacaagc tccattacaa
    14161 tctttatcga gaaggttctg gacgtgcagg tttccttcgt cgacattggg gcgtcaagaa
    14221 ataagaagcc gaaaaagcta cttatggaac accgtatatc agaggttgta aaaccagcat
    14281 tttgagctaa tactgagggt cagctgattg aacaccattt agtggagagg ttgtgaacct
    14341 gtcgcctagc tccaagtcac aaggatttgg taagagaaca ctatatatca gaggttgtga
    14401 caagtcgttt gtcacaacct tttttcaata cctaagatga aggacaatca gcaacgcact
    14461 gctcaaaaaa ggaatgaaag gcagcttttc caactttttt ttatgacaga taaagtaaac
    14521 agaaaaaacg gatattccaa acagactagc aagaaacaag atctgaagca actcttctag
    14581 agacaagcca ccactccaag aaagcatcaa cagagtatcg ccagctccga gataggattg
    14641 accatagcga aggcacacac cgcctacgat aaacgttaac agcacacttt gccattggaa
    14701 gacgccggcc tgcaaccaaa aaatccataa aattgcccaa gaacagaata gcgtgtgaga
    14761 atggaccatg aagtaaaata aatccatcaa cgaaaatagc aaagccgaaa gcatccatat
    14821 gaacgtcgaa aaatttcttg cggaagaaaa agtaaagaac caggcaaaaa gaccaccaca
    14881 taaggcttct gccaacaaat agtagaaggg caaccgacac ctgcaatagc ggcagcgaaa
    14941 tcgttggacg atgatcgaga gaatcggaat caattcatac caagaaagcg aacgacagca
    15001 tttgacacaa tgagatctag gataaataat agagtgacct aacggaatcc gctgggcggt
    15061 taaacataaa aatgatccga tacagcaacc aatgataaaa taaagtaaca cgccatctcc
    15121 cccttttcaa aaccaagata cgcaagctta aactaaattt ttgtacatac ataaaatttg
    15181 aggtgaaaaa atgaacgttt caatcggatt agatatcgga accacacaaa ccaaagcagt
    15241 tgctttcaca gatgaagccc aagaagcagc atccgcttat tttcgctacc ctttgatcca
    15301 agaaacacaa gggatggcag aacaagatca ggaattgatc tttcaaggtg tctgcaccgt
    15361 tataaaagaa gtaattaaac agttgccttc agacacaatc attcaatttg tctccttctc
    15421 ctctgcgatg catggcctga tattgttgga tgaagagaaa acaccgttat ctcgaatgat
    15481 cacttgggca gataaccgag cagcggacga agcagaaaaa ctgaaatata ccgtgcttgg
    15541 caaagaattt tatcggcaga ctgggacgcc gattcatcca atgacaccat tgaacaaaat
    15601 caagtggctg caaaacagcc aacctgaatt attcaaaaaa actgcttatt ttgtcggtat
    15661 caaagactat atcttttata gattgtttgg cgaactgatc agcgactacg gaacagcttc
    15721 aggaactgga tattttgata tccatgaatt ccaatgggca gaagaagtgc ttgactactt
    15781 gaacattcga atagagcaat tgccgcaagt ctttccttcc acctatcaag taacaggtct
    15841 accatcaaaa acagcagcag aactaggttt gccaaatgat atatcatttg ttttgggagg
    15901 ggcagacggg ccgctttcta atctagggtt aggtgctttt ggtgatacga tcgccgcttt
    15961 gaccgtagga acgagcggag cacttcgttt tattactact cagcctcagt tccatccaga
    16021 aatggagacg ttttgttatg ttctggatca gaaacattgg gtagtgggag gagcgactag
    16081 caatggtgct ggcatctttg attgggcagg gcatacattt ttacaggaag tcgttcaatc
    16141 tgctaaagaa aaaggagaaa atccatatga tgcactgctt tcggctatcg cttttgtgcc
    16201 agcgggtgcc aatggtctga tttttcatcc ctatttactt ggtgagcgag caccgctttg
    16261 ggacgcagat gcgtttggca gttttatcgg tttacagcgg caacatacag aaaaagaaat
    16321 gatgaaggcg attctagaag ggatctgtct caacctttat cggattttaa aaggagtttg
    16381 ccaggaaaat cagctcacag agatccgtgc tacaggcggg tttgctcaat ctccgatttt
    16441 tcgtcagatg atggctgatg tactgggtta cccgcttgtt ttccctactg tcacagaagc
    16501 atccgcatta ggagctgtga ttttaggctg gagaagcatc ggaaagatcc aaagtatcag
    16561 tgaagcacag gaattgatag aattgaaaga gcaagttgga gtacaagaaa ctgatcataa
    16621 aaaatatcag aaaatctatc cattatttgt ttcgacacaa aagcatttag cacagactta
    16681 tcagcaattt gctcaactgc gagaagaatt agctgattct gaaaaatgat tggctttacg
    16741 gataaaaaag cgaactgaaa atcgttctca ggtaaatgat tttgttgtac ttttcattga
    16801 aagtctacta ttattttaag tgtaagaaaa tgttaggagg caagaaagat gaaattccaa
    16861 caaaccaaag aagtattaaa tcaattagtt gcagatctaa gccaaatgtc tgttgttgtt
    16921 caccaaacac attggtacat gagaggacca ggtttcctta cattgcaccc aatgatggat
    16981 gagttcatgg atgatttgaa cgctcagtta gatgaagtat ccgaacgctt gatcacatta
    17041 gacggttcac cttattctac attgcgtgaa tttgcagata acacaaaaat ccctgatgaa
    17101 attggccgct gggatcgtac tattccagaa cgtttggaaa cacttgttgc aggctatcgt
    17161 tatctagcag atctttacca aaaaggaatc gaagtttctg gagaagaagg cgacgatcca
    17221 acacaagata tctttatcga attcaaaaca gccacagaaa aacgtatctg gatgatccaa
    17281 gctcatttgg gcaaagcgcc agagatcgac gcgtaaaaac tgttgttgaa cttgaccgaa
    17341 aaagaggttg tgaaatcagc gttttgacct gagtattaga gaaacaaacg tcaaaatagt
    17401 tcctcatatt ttgccgtttt ttgagctaat accgagggtc agctgattga acaccgttta
    17461 ccaagaggtt gtaagcctgc cgttcagctc cgagtcacaa gaaacaattt taaaaaacag
    17521 cttcttatgt tttatggata ttgtgacttg tgaccgagga gttggcagga gaacaccgtt
    17581 tattcaaaat aaggggttgt gacaagactt ttgtcacagc ctctttttgt ttttcatttt
    17641 gacaggaaac gaccctttta tataaaagaa agaaaagaag taaagtttca tataaatttt
    17701 tcaatcaagc agaaaaacgt tgacattgac tagattagcg ggtaatatgt taaatggtat
    17761 tgtttgcccc acaaagagcc ccggaaactc gaagtgtatg tcaaacatca gaacttaaat
    17821 tggaggaaaa taacgtgcgt acaacatata tggccaaacc aggcgaagta gaacgtaaat
    17881 ggtatgtagt agacgcaact gatgttccat tgggacgtct ttcaacagtt gttgcatctg
    17941 tactacgtgg taaaaataaa ccaactttca cacctcattt ggatactggc gatttcgtca
    18001 tcgtcatcaa tgcagacaaa gtgaaattaa caggtaaaaa agcgactgac aaaatttatt
    18061 accgtcacag caactaccca ggaggattga aatcaatcac tgctggcgaa ttgcgtgcta
    18121 aaaattctcg tcgtttgatc gaaacttctg taaaaggtat gcttcctaaa aacactttag
    18181 gccgcaaaca atttacaaaa ttgaacgtat acggtggagc agaacatcca catgcagcac
    18241 aacaaccaga agttttagac atcacaaact taatctaata ggagggaatt tcattggcac
    18301 aagttcaata tatcggcaca ggccgtcgta aaaacgcagt tgctcgcgta cgtctagtac
    18361 caggaactgg caaaattact gtaaataaaa aagatgttga agaatacatt ccacatgctg
    18421 acttgcgtga agtaatcaat caacctttcg ctgttactga aacaaaaggc gcatatgacg
    18481 tatttgttaa cgtaaacggc ggaggatacg ctggacaatc aggagctatc cgtcatggta
    18541 tcgctcgtgc attgctagaa gttgatcctg acttccgttt agctcttaaa cgtgctggct
    18601 tacttactcg tgacgcacgt atggtcgaac gtaaaaaacc aggtcttaag aaagcacgta
    18661 aagcttcaca attctcaaaa cgttaatatc ctttatatat caacgtttct ggcacttttc
    18721 aattatttgg aaagtgcttc ttttgttttt aagaacacat ttaagaacac atatcaatca
    18781 cacttattca atcctctctg aaaagcttca aaaacagcct tatcaactat atctggtgtg
    18841 ttcacatata tctttgttgt agaaatatct gagtgtgtta aagcatttga aatttgattc
    18901 atatctgctc caccttgtct agctatagtt gcataagtat gacggagttt gtgtggagat
    18961 aaatgaacta agtcaggatg ccttctttta attgaattga ttcgatagtt taggtaatcg
    19021 gcatgaacag gacaatttac ttctccagat ggtttagtat aagtaaagag atactgatct
    19081 aacgtttgct taatttttaa tttcaacaat tgatcagctt gtaccgattt ccattcactt
    19141 agcaatgtca ttacaacttc tggtacttga atgacggtat cttttcgacc ttttgtaaac
    19201 ttttttcttt gatatttatc taaagtatgt ttaagacgga ccgtttgttt ttcaaaatca
    19261 atatatttcc attgcaaagc gtaagtctca ctttttctat caccaagata cagggtaatc
    19321 ataaaaagtg tgtaatcatg aaaagtaagt aaatgattat taaaatcatc atttactgct
    19381 tctatccaat tagtaagttc ttttgatgat agtgcttgct taccttcttg tattcttttt
    19441 tcttctaaag catttttttt aggtgcagtg atactttgaa taattttagt agttcgatta
    19501 aattcgatat aatttagtat ttctgcaata tcgaacatgg aacgaacata tccttttatg
    19561 atttttatat ttgcatattc tttcgatttt ttggttaatt catctgatat tatctcagta
    19621 ttgatattta gataattcat tgcgtattca ccaaacatag gtaaaatatg caaccgaaat
    19681 atatcttttg tattactaat ggttatatca ctaggaggcc tgtttgatct aattgtttga
    19741 cctaattcat atctaggaag ccatttagat tcgtaaaatt ttttaaacgt tatttttcct
    19801 tttaattcta atgagtttgc attctcctct ctcaaaactt tttctatttt cttcttcata
    19861 tcattttcgg ctttaatcgc caattgccta tttggaaatg ttttttgata cttttctgat
    19921 gcaattcctg ttatttcttt aaaccttttt ggatatctca gattagtttt atatttccca
    19981 tttgaaagct ttgtaggcaa aatcctcact ccttaaaaaa agtaaggcag tatatttcac
    20041 taattttagt atatgcggcg agaagaaaat ttcaagttat tttaaatgta ttcataaagt
    20101 ctttaatatc ttctacgtca aagcgaacta caccaccaac tcttattact gggaaattat
    20161 atatacgaat ccacttatct agagtattat tatcgattcc tagaaatgca caggtttgtt
    20221 ttttattcag gtagactctg gattctataa aatcatctcc taaaatagta gttatgaaat
    20281 ttttgaattg ctttgggtaa gcacatagga atttcacctc tccccagttt acggcgagcc
    20341 ttacggaagt atcattattt gctgctattg tcatggcatt ttgttttccc agaacaaaaa
    20401 gaaatctcta agtatttgtc gctaatcagg tcatggcgta cttgagtttc tagctcgaat
    20461 agcagggatc gttattcaaa aattgtattt aattgtcaat gatcaaaaat agtttttttc
    20521 ctattcacta ttaataacga taaacatgta taaaaagaca cactacgaaa aaagttaaat
    20581 ggagtttact gattttcata tttgtatatg gaacagacat agtcttgaat aatttttata
    20641 aatgttttga tgcttatcaa cttattcaac ccgctatcca cttcaagtat aatttttcca
    20701 tacgccaata aagtagtttt aggagcatgt tctcatttga gaacgctcct tttttatata
    20761 ttttgaaagc tacttactat acgcgtagtt tcaaatagtt ttgtcattct aatcttcagc
    20821 aacagaaaaa aatctaaact ataaaattta gggttgacta aaatataatt tttgtatacc
    20881 atatgatcgt gataatcatt atcatttaga aggagaaagt tatgttagaa gtgaaaaagc
    20941 ttaatgtttg ttataacgat tttttagcat tgaacgagat ttctttaaaa atacaagaag
    21001 gtgctttaac aggtattatt ggtccaaatg gtgctggaaa atccacattg cttaaagcaa
    21061 tgttaaatat tatccctcat cagggagagg tctccattaa taatgaagat atcaataaaa
    21121 aactggtgaa gattgcatat gtagagcaaa aagctgatat tgattttact tttccaataa
    21181 aagtaaaaga atgtgtatct atgggaacat atgccggaat gaaagttttt caaagaatta
    21241 aaaatgcaga atggcaaaga gtttcaaagg ctttggaaaa ggtcgatatg ggaaaatatt
    21301 ctaatcatca aataggagaa ttatctggag gacaatttca acgtgtactt ttagcaagat
    21361 gtcttgctca aaatgcggat ttcatatttt tagatgaacc atttgtaggg atagacttag
    21421 ttagtgaacg aataattatg gatacgttaa aaaaattgaa acatcaggga aaaacagtgc
    21481 taattgtgca tcatgattta agcaaggtaa agaagtattt tgataatatt attcttttga
    21541 atcgaaatct aattgctcac ggaagtgtag aatctgtatt taatgaagaa aatttaaaaa
    21601 aagcctatgg agatactata tttatt
//
LOCUS       DAZABD010000047        19572 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000047 DAZABD010000000
VERSION     DAZABD010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.047"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            40..1524
                     /locus_tag="ACHALG_001884"
     CDS             40..1524
                     /locus_tag="ACHALG_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HFN2587268.1"
                     /translation="MKKLSQKRLAQIIIDSRKSLKMTQQQLAVATGINRALISRLESL
                     DFIPSIQQLESLAEVLNFEPTDLFINESNKSNRTKHQSKNIAVAGTGYVGLSIAILLA
                     QHNQVTAVDIIPEKVKQINNKKSPIQDDYIEKYLAEKNLNLIATTDGATAYKKADFVV
                     IAAPTNYDSKQNYFDTSAVESVIELVLAVNPKAIMVIKSTVPVGYTESIREKYNTKNI
                     LFSPEFLRESKALYDNLYPSRIIVGTDQNDKTLLEASHTFADLLQEGAIKKDIETLLM
                     GFTEAEAVKLFANTYLALRVSYFNELDTYAEMKELNTKDIINGVCLDPRIGTHYNNPS
                     FGYGGYCLPKDTKQLLTNYNDIPQNMMSAIVDSNRTRKNFIADRVLQKTGYYSYDDDN
                     TFDLSLEKEVIIGVYRLTMKSNSDNFRQSSIQGIMKRIKAKGATVIIYEPTLKDGSTF
                     FGSKVINDLEKFKSISHSIIANRYDSILNDVKEKVYTRDLFQRD"
     gene            complement(1639..1773)
                     /locus_tag="ACHALG_001885"
                     /pseudo
     CDS             complement(1639..1773)
                     /locus_tag="ACHALG_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209342.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1846..2972
                     /locus_tag="ACHALG_001886"
     CDS             join(1846..2074,2074..2972)
                     /locus_tag="ACHALG_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFN2587269.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PNQSTGLTGKEAADLRKENARLKEELEILKKAGSHILSKDLKSLVRFIEKWCKEYTVS
                     LLCRLLEIPRSVYYFYKNKSLTATEVRNNQLKKKISTIFFDHKQRYGATKIHQVLLKE
                     GISVSLKHVQKLMKQLNLRSIVVKKYRPQRPVQPVVLKENILNQDFSTKTICEKWVAD
                     ITYIPTKKNGWCYLSSIMDLHTKKIISYTFSKRMTVDCVLQTFNQAKQRYHIPEGMIL
                     HTDLGSQYTAIEVENWLENNKIRHSYSRKGTPYDNAGIESFHASLKKEEVYTTTYSDF
                     EEANRSLFSYIEGFYNRNRIHSSIHYLTPQEFEDLETEKMS"
     gene            complement(3016..3258)
                     /locus_tag="ACHALG_001887"
                     /pseudo
     CDS             complement(3016..3258)
                     /locus_tag="ACHALG_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686363.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
     gene            complement(3282..4478)
                     /locus_tag="ACHALG_001888"
     CDS             complement(3282..4478)
                     /locus_tag="ACHALG_001888"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFN2587270.1"
                     /translation="MKKKIFFDMILNIVATSIPTVVLQLLILPSLSNYMDGNAYGLMV
                     TILAVLNVVPSTIGNVLNNVRLLHEESYKKDNEQGDFQILLTIFQAINLLIMVTVSIY
                     YLGTKDITGILLLIAVGILWLGREYHIVAFRLKLDYIAILVNNLCLVAGYGIGYIFFM
                     KNGYWQIIYLLGYILSYLHIVIASPIVKEPFTKTKRLRIVFNDSAFLLISNVLARAIN
                     YADKILLYPLLGGTMVSVYYAATVFSKVVSLAITPINSVALSYLSKINKKTNSLFKWT
                     YIVGIVVCGIGYIMTIILSRPVLSILYPKYVDAAMQYIWITSGTIVVSVLTSMITPFV
                     LRFFDIKWQIWINGITTIMYIIISLSLLKLGGLMGFCIGALLANIIKLLITTYVYLKK
                     TSRSSE"
     gene            4668..5987
                     /locus_tag="ACHALG_001889"
     CDS             4668..5987
                     /locus_tag="ACHALG_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HFN2587271.1"
                     /translation="MTSLHKNQVKFNSNLTISHTGGRLSSDSGLVLVQEVMNTFGFSH
                     VAKQWLHIKDKRVYFTHDNLAILEQLIMQLIAGYSADSSANLLRQDPVFQAVLGKKEL
                     ASQSSISRFLDRFTEENMDQLQALNQSLIDKARLIRNDTELIIDVDSTHSDTFGRQEQ
                     TDYNAHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKAFIDPLLHHYKSTLPH
                     TEILVRGDSGFATPEVYESCEATESQYVIRLKSNRRLSQLAEHSVLYGDNKKWEDREI
                     QYFSLPYQAQSWSKPRRVCIRSIREAGELLFHHAFIVTNLSDNVSPEVIFSLYGKRGT
                     MENFIKEAKSGFYFDKTDSPRFLENHVRMMISVLAYNLVNFLKTIGFEQVNRGMTIHS
                     IRLTLLKVAGKLVKTGRQVYLKLSSYHVYQTEFYKVFERLRRSRQWI"
     gene            complement(6184..6900)
                     /locus_tag="ACHALG_001890"
     CDS             complement(6184..6900)
                     /locus_tag="ACHALG_001890"
                     /inference="COORDINATES: protein motif:HMM:NF014571.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046353 - aminoglycoside
                     3-N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAC(3) family N-acetyltransferase"
                     /protein_id="HFN2587272.1"
                     /translation="MDEIGVKKGDVLIVHASWRALYMLDMCPEDVIRLLLDKIGKEEG
                     TLLMPAYGEDNSSFDLNKTKSVAGVLSESLRNYPGTMRSCFPKFSMCGYGKHAEEILS
                     KHKNSLYQFDENSPYSIATKEYNAKVLLMGMGKRPHKITVFHCASYALKNSIDYYKEC
                     YSNYKTAYVLSDVGGTVQYLDRMPGYNNNKHVFKKLFESIPNKCIVSKKRLNLIIFNA
                     IDAYNVAWEFCKNGGKIYTT"
     gene            complement(7006..8361)
                     /locus_tag="ACHALG_001891"
     CDS             complement(7006..8361)
                     /locus_tag="ACHALG_001891"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008755 - O antigen polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polymerase"
                     /protein_id="HFN2587273.1"
                     /translation="MLTNIKNLLLKPNKVLQSIYIIAFRVLLDYMYCNIVSPLYSYIG
                     MTAEQSTFYYMVSWIMVICYILMMFTIIDVEKNGAVVLSIILICSAVPTTTIVAFLGH
                     NLEFAVLNVLYWFFMVAFYKHKGRKIVLGKNTIKRSSLLIYAVFLFFAAVLLLICVAY
                     TGIHVSFNLYDVYGTRAAFKSDKLPTILQYLFSACIIVFPVIIVYGLNKKKRILALAA
                     TICQLLAFFADGRKSTIFVLIATILGYYFIHNLTARMIPAIMMGVTVIGFIEKICIGT
                     ENFINLVARRLFLVTAYLQYAYFDFFSNNVKDYFRQSIVGRLGFSSPYTKDIPTLVGG
                     QYYVNNSYANNGLFADAYSNFGAVGVVILPILIVCALKLLDRVCEGLTSGICMGIIIV
                     SAYTLLSSSFFTVMLTHGFLLGCIIIYLVPRFSYQRDVVVENEGMKGKYDDKKSITKY
                     S"
     gene            complement(8355..9482)
                     /locus_tag="ACHALG_001892"
     CDS             complement(8355..9482)
                     /locus_tag="ACHALG_001892"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFN2587274.1"
                     /translation="MKILHINCNYILSSLHQCLIEKLNAAGFSNDVFVATYDKKRSIV
                     NTKEYVKIEECFNKWDRINFDFKQAKILKKIEEDFDLEKYDCIHAYTLFTDGNSAMKL
                     SDKYGIPYVVAVRNTDVNDFFAKRFLLRNRGIEIMRKAQKVFFLSNVYRETVLNNYVP
                     EKYRNEIYEKTVVIPNGIDDFWFENQPEDTIKKSEKKDFIRLVYAGRIDRNKNIPTTQ
                     KAMKILKKKGINSELLVIGKVDDKTELKKIISEDSTCYEDAKPKEQLIDAYRNADIFV
                     MPSFTESFGLVYAEAMSQGLPVIYSKGQGFDGQFAEGVVGYHVDAHDPEELCENIIKI
                     LKRYNEFPEACMQASKKFRWDNLSKEYVKIYQEIMENKNSC"
     gene            complement(9505..10824)
                     /locus_tag="ACHALG_001893"
     CDS             complement(9505..10824)
                     /locus_tag="ACHALG_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HFN2587275.1"
                     /translation="MSLYEKIINGEEKIAIVGLGYVGMPIAVAFAKKAKVIGYDLNEK
                     KIELYKSGIDPTNEVGNEGVKLTTVEFTSDEKKISEAKFIIIAVPTPVNTDHTPDLTP
                     VIGASEIVGRNLTKGSIIVYESTVYPGCTEDVCIPILEEKSGLKCGKDFKVGYSPERI
                     NPGDKVHRLENIHKIVSGMDEDSLNEIKNVYDLVIEVGTYPVSNIKTAEAVKVVENSQ
                     RDINIAFMNELAMVFDRMGIDTNEVVDGMNTKWNALGFRPGLVGGHCIGVDPYYFTYE
                     AEKLGYHSQIILNGRIVNDSMGAYIADTAVKTMVVAGQSPKKSKVVILGLTFKENCPD
                     TRNSKVEDIIKQLKTYDIDPVVVDPWADEKDAFREYGVKLSELENAKNADCIIVAVAH
                     NEFKKMSLDEIKALFKDCPDNEKVLLDVKGLYSVSDLKKSGMNYWRL"
     gene            complement(10845..11612)
                     /locus_tag="ACHALG_001894"
     CDS             complement(10845..11612)
                     /locus_tag="ACHALG_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013851740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFN2587276.1"
                     /translation="MEKGLVSVIMPSWNTGNFIAESIQSVINQTYEKWELIIVDDCST
                     DNTDEVVGTFADKRIKYLKNEKNSGAALTRNRALREAKGEWIAFLDSDDLWKPKKLEH
                     QLKFMKKHNLIFSYHEYVKIDEDSQPLNIYVSGPPIVTKRKMYNYGYPGCLTFMYSAK
                     AMGLVQIKDIKKNNDYAILLKLCKKADCFLLKENLAEYRIRKKSISHDKLSKKLKSHY
                     DLFHMCDEKIAPVALWYACWNMFFGVMKKRKYEKIIK"
     gene            complement(11622..12272)
                     /locus_tag="ACHALG_001895"
     CDS             complement(11622..12272)
                     /locus_tag="ACHALG_001895"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018659149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFN2587277.1"
                     /translation="MRTEEVRPYYDSLKKKKISLALKRGFDVFAASVMLVTFSPVLIT
                     ISIMIVRDSKGGVFYRQERVTQYGRKFKIFKFRTMVANADKIGTQVTVSNDSRVTKVG
                     EKLRKYRLDELPQLINIILGDMTLVGTRPESTHYVKKYTSEMYATLLLPAGVTSEASI
                     KYKDEAELLDKADDVDKVYVEKVLPEKMKYNLESIRKFSFINDIGTMIKTVFAVIR"
     gene            complement(12333..13553)
                     /locus_tag="ACHALG_001896"
     CDS             complement(12333..13553)
                     /locus_tag="ACHALG_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HFN2587278.1"
                     /translation="MNKLEKRQIPFSPPDITEKEIEQVSEALRSGWITTGPKTKELER
                     EVADLCGVNRAVCLNSQTACAEMTLRLLGIKEGDEVITCAYTYTASASVVCHVGAKLI
                     LIDTQRDSLEMDYEQLENAITEKTKVIIPVDLGGVPCDYDRIFSIVERKKGLFHPVNK
                     IQESIGRVIVMADAAHAFGATWKEKPVGSIADFSNFSFHAVKNFTTAEGGAAVWKDIE
                     GIDNEEIYHQYQLLSLHGQSKDALAKTQLGAWEYDIIGPWFKCNMTDVVAGIGLAQMK
                     RYKGLLERRKEIIGKYDSAFKPIGIEVLNHYTENHQSSGHLYITRVPGITLEQRHEII
                     VKMAEVGVACNVHYKPLPMMTAYKNMGFDIKDYPNAYKRFENEITLPLHTKLTDEEVD
                     YVIKQFSRIVKEYI"
     gene            complement(13567..15384)
                     /locus_tag="ACHALG_001897"
     CDS             complement(13567..15384)
                     /locus_tag="ACHALG_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HFN2587279.1"
                     /translation="MFVLTRRRKIATLVFVDSLLLIAANIAAAKFMKPFVAIPMDLIL
                     ISIGLSIGFYLFYGSLFKVFTRINRYTNLREIIAIFGSLSASAVSSIIILLFINRRYS
                     LRLVIFAYLLSLLLIIGSRLIWRIYVETKNMRYVSADSAKNTLIVGAGEGGRILYNSF
                     LGSKTAQDIHVVGFVDDDPNKRNTYLSGKKVLGALKDIPELIEKYDIQMVTIAIPSLS
                     RKKLRRIFELVESARVKVNTMPSIEELASGKISVSKLKTIDVVDLLGRDEVKLDIESI
                     KDQITDKVILVTGAGGSIGSEICRQIIQFNPAKLLLLGHGENSIYLIDRELRTHHQNC
                     PTEIVPIIADIQDREKINEIMEQYHPDIVYHAAAHKHVPLMEYNPKEAVKNNIFGTKN
                     VAEAAKAARVKNFVMVSTDKANNPPNVMGSTKRIAEMIVTGLNEESCTKFSAVRFGNV
                     LGSRGSVIPVFREQIAQGGPVTVTDFRMTRYFMTIPEASRLVIQSGALAKGGEIFILD
                     MSEPVKIVDLAKNMIRLSGYSEDEIEIIETGIRPGEKLYEELLLDKERNDEAVYEKIF
                     VGNIKGYSMQTVMDFVKSLPQDDEQLAKEIVTFANASNK"
     gene            complement(15397..16161)
                     /locus_tag="ACHALG_001898"
     CDS             complement(15397..16161)
                     /locus_tag="ACHALG_001898"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HFN2587280.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTMEVPLYAEQLFFELLELGKTPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYKQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPIYEEVKKKKFGLF"
     gene            complement(16189..16887)
                     /locus_tag="ACHALG_001899"
     CDS             complement(16189..16887)
                     /locus_tag="ACHALG_001899"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HFN2587281.1"
                     /translation="MARTQKQKATQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPREGKSTTSANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAS
                     GLSTALSSSGSVVDVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(16899..17678)
                     /locus_tag="ACHALG_001900"
     CDS             complement(16899..17678)
                     /locus_tag="ACHALG_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HFN2587282.1"
                     /translation="MEETISLKEIFETLRKHLATIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQAISTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGSAPARPKATNDKTAPSRRSRSRV"
     gene            complement(17694..18638)
                     /locus_tag="ACHALG_001901"
     CDS             complement(17694..18638)
                     /locus_tag="ACHALG_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFN2587283.1"
                     /translation="MARRRRKSRGKKIFLIILAVLVVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFTIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(18684..19463)
                     /locus_tag="ACHALG_001902"
     CDS             complement(18684..19463)
                     /locus_tag="ACHALG_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587284.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRITYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEKIRNEKISVAYATKAQTVAEQMGYSNISY"
BASE COUNT         6014 a         3938 c         2926 g         6694 t
ORIGIN      
        1 aaatcatttg ttattaatag taacaaaaag gagtatatca tgaaaaagtt atctcaaaaa
       61 cgtttagctc aaatcattat tgattctaga aaatctctaa aaatgacaca acaacaactt
      121 gcagtagcta ctggtatcaa ccgcgcctta attagtcgat tagaatctct cgattttatc
      181 ccttcaatac aacaattaga atcattagcc gaagttctaa attttgaacc aacagatcta
      241 tttataaatg aatcaaataa atcaaacaga acaaaacatc agtcgaagaa tattgctgta
      301 gctggtactg gttatgtagg tctttccatt gctattcttc ttgctcagca caatcaggta
      361 acagcagtag acattattcc tgaaaaagta aaacaaatca ataataagaa atcgccaata
      421 caagacgatt atattgaaaa atatctagct gaaaaaaatc ttaatttaat tgcaacgaca
      481 gatggagcaa ccgcttataa aaaagcggat tttgttgtca ttgctgcccc aacaaattat
      541 gatagtaaac aaaattattt tgacacttct gctgtagaat cagtaattga attggtatta
      601 gcagtcaatc caaaagcaat aatggttatc aaatcgacgg tacctgtagg ctataccgaa
      661 agcattcgtg aaaaatacaa tactaaaaat attctattca gcccagaatt tctcagagaa
      721 tctaaggcat tatatgacaa tctataccct tcgcgaatta ttgttggcac ggaccaaaat
      781 gacaaaactt tactagaagc ctctcatact tttgcagatt tactccaaga aggagcaatc
      841 aaaaaagata tcgaaactct tctcatggga ttcacagaag ctgaggcagt taaattattc
      901 gctaatactt accttgcact tcgagtatct tacttcaatg agctggatac ttatgcagaa
      961 atgaaagaat taaataccaa agatattatc aatggagtat gtctcgatcc aagaattggc
     1021 actcattaca ataatccctc ttttggatac ggtggctatt gtttaccaaa agacacaaag
     1081 caacttctaa ctaactataa cgatatacct caaaatatga tgagtgcaat tgtcgatagt
     1141 aaccgaacaa gaaaaaactt tatagcagac cgcgtgcttc aaaaaactgg atattattct
     1201 tacgatgatg acaatacttt cgatttgtct ctagaaaaag aagtgattat tggagtctat
     1261 aggcttacaa tgaaaagtaa ttcggataac ttccgtcaat cttctattca aggtattatg
     1321 aaaagaatca aggcaaaggg agccactgtg ataatttacg agccaacact taaagatggt
     1381 agtacatttt ttggttcaaa agtaataaat gatttagaaa aatttaaaag cataagtcat
     1441 agtattattg ctaatcgata tgatagtatt cttaatgatg taaaagaaaa agtatacact
     1501 agagatttat ttcaacgaga ttaatatagt gatctatttt gatactataa ttcttaggct
     1561 ctttgtcaaa tgatgttggt gaagagatat cctagaatga ttttgaccag aaagaaaaaa
     1621 tttctttctg gtcatttttt attttatgag caatgctttc acatatcgaa tcatgattcg
     1681 caatttaaag ttataaaagc ttcgaaactc ataggcattt cgttttaata cctcgatatg
     1741 attattcaaa cactccaaag gcccatttgg tgttagtatc aaatcttaga caacttttcg
     1801 tagagacaga aataaattaa acttatctac gagaggatgg attttatgac ccgatacgaa
     1861 gaaaatttca aacagatgat tgttgagctg aatcaaactg gacgttctgt tcgaggattg
     1921 gcgaaagaat atggcttatc tgaagcaacg atttacaaat ggaaaaattt atatttacca
     1981 aatcagtcca caggactgac cggaaaagaa gcggctgatt tgagaaaaga aaatgctcgt
     2041 ttgaaagagg aacttgaaat attaaaaaaa gccgcagcca tattctctcg aaagacctaa
     2101 agtcacttgt tcgattcatt gaaaaatggt gtaaggagta cactgtttct ttgttgtgtc
     2161 ggttattaga aattcctaga agcgtctact atttttataa aaataaatcg ttaacagcta
     2221 cagaagtcag aaataaccaa ttgaaaaaga agatttcaac gatctttttt gatcataaac
     2281 agagatatgg tgcaacaaaa atccatcaag tgttgctaaa agaaggaatt tcagtatctc
     2341 ttaagcatgt acaaaaactg atgaaacaat taaatctaag gtcaattgtg gttaagaaat
     2401 acagacctca acggcctgtt cagccggttg ttttaaaaga gaacatctta aatcaggact
     2461 tttctactaa aactatctgt gaaaaatggg tagctgatat cacctacatt ccaactaaga
     2521 aaaatggttg gtgttactta tcttcgatta tggatttaca cacgaaaaag attattagct
     2581 atacattttc taaacgaatg actgtcgatt gtgtgttaca aacattcaat caagcaaagc
     2641 aacggtacca catcccagaa ggaatgattc tacacactga tttaggtagt caatatacag
     2701 caatcgaagt ggaaaattgg cttgaaaaca ataaaataag gcattcctat agtcgcaaag
     2761 gaacaccata tgataatgca ggaattgagt ccttccatgc atcattgaaa aaagaagaag
     2821 tatacacgac gacttactca gacttcgaag aagcgaatcg atctttattt agctacattg
     2881 aaggatttta taatcgtaat cgaatccata gttcgattca ttacttaact ccacaggagt
     2941 ttgaagattt agaaacagag aaaatgtctt agcagtctct actaaaatat gtccaagata
     3001 ttgactcaaa tccagtgcag caataactac aaaatccgca tccttatacg cagcctctgc
     3061 atctaacgta gctgtcagct ttaactcttt ttcctttagg tatttttcaa tatactcatc
     3121 ctgaatcgga ctaatccttt tattaatctt ctctactttt tccggaacaa tatctactgc
     3181 cattacctca tgattttgag ataataatgt ggcaatagaa agtcccacat atccagttcc
     3241 tgctactgct attttcataa aatatcccca ctttctacta tttactcaga acttctactt
     3301 gtttttttca aataaacata tgtagttatt agcaatttaa ttatatttgc caacaatgct
     3361 cctatacaaa atcccattaa tccgccaagt tttaataaac taagagaaat aattatatac
     3421 attattgttg taattccatt aatccaaatt tgccatttaa tatcaaaaaa gcgtaggaca
     3481 aacggagtaa tcattgatgt caaaacagat actactattg ttccggaagt aatccagata
     3541 tactgcatag ctgcatccac atatttcgga tacaatatac tcaataccgg tctacttaaa
     3601 attatagtca ttatatatcc aataccacac acaacaattc caactatata cgtccattta
     3661 aaaagggaat ttgttttctt attaattttt gaaagataac ttaatgctac actattaatt
     3721 ggtgtaattg ccaagctaac aactttgcta aataccgtag ctgcataata tactgaaacc
     3781 atcgttccgc ccaaaagtgg atacagcaat atcttatcag cgtaatttat tgccctagct
     3841 aatacattag aaatcaataa aaatgcagag tcattaaaga cgattctcaa tctttttgtc
     3901 tttgtaaaag gctcttttac tatcggacta gctattacta tatgtaaata acttaaaata
     3961 taacctaata aatatataat ttgccaatat ccatttttca taaaaaagat atagcctata
     4021 ccatatccag ccacaagaca taaattattg actaaaattg ctatataatc cagttttaat
     4081 ctaaatgcca caatatggta ttctctacca agccataaga tgcctactgc tataagtaat
     4141 aatataccgg ttatatcttt ggtacctaaa taataaattg ataccgttac cattatcaac
     4201 aaattaattg cctgaaatat tgttaataaa atttgaaaat cgccctgctc attatccttt
     4261 ttataagatt cctcatgtag caaccttaca ttatttaata catttccaat tgtagatgga
     4321 actacattta aaaccgctaa tattgtaacc atcaatccat aagcatttcc atccatataa
     4381 tttgataatg atggaagtat caacaattgc aataccaccg taggaattga tgttgcaaca
     4441 atattaagga tcatatcaaa aaaaattttt ttcttcatat cattcttccc ttttcaaata
     4501 tttacttggt atcctgaata attcatactt tgacttcaaa ccgtctgaaa ctgcctaacg
     4561 gcagtcttca ttttcttttt cattttcacc tgttaagtga tgcaaaaaga accccattct
     4621 gatatggtaa aggtgtcgaa accaaccata agaaaggagt tcttctcatg actagcttac
     4681 acaaaaacca ggtaaaattc aattcaaatt tgactatttc acatacaggt ggtcgcttat
     4741 cgagtgattc gggtttggtc ttagttcaag aggtgatgaa taccttcggc ttttcacacg
     4801 tagctaaaca gtggctccat attaaagaca aacgtgttta cttcacacat gataacttag
     4861 cgatattaga acaactcatt atgcagttaa ttgctgggta ctcggcagac tcatcagcta
     4921 atctattaag acaggatcca gtctttcaag ctgtactcgg taaaaaagag ttggcctcac
     4981 aatcgtcaat ctcacggttt ttagatcgtt tcaccgagga gaacatggat caactccaag
     5041 cattaaatca gtcgctgatt gataaagcgc gtttgattcg caatgacacc gagttaatca
     5101 ttgatgtcga ttccacacat tcggatacct ttggacgcca agaacaaaca gattataatg
     5161 cccattatca aacctacggc tatcacccat tagttgcatt tgacggattg accggagatt
     5221 tcctaaaagc tgaactgcgt tcaggcaatc agtacacgtc taaaggggta aaagccttta
     5281 tcgacccgct gttacaccac tacaagagca cgttacctca taccgagata ttggttcggg
     5341 gtgacagcgg cttcgccaca ccagaggtgt atgaatcttg tgaagcaact gaaagccagt
     5401 acgttatccg attaaagagc aatcggaggt taagtcagtt agctgaacac tctgttcttt
     5461 atggggataa taaaaaatgg gaagaccgag aaatacagta tttttcactc ccttatcaag
     5521 cacaatcctg gtcaaaaccc cgtcgcgttt gtattcgatc aatccgtgaa gcaggagagc
     5581 ttctttttca ccacgcattc attgtgacga atctatccga taatgtctcg cctgaagtca
     5641 tattctctct ttacggcaag cgtggaacaa tggaaaattt catcaaagaa gcgaaatcag
     5701 gcttttattt tgacaaaaca gatagtccgc gtttcctgga aaatcatgtc agaatgatga
     5761 tcagtgtcct ggcttacaac ctcgtcaatt ttttaaagac cattggattt gaacaagtga
     5821 atcgggggat gacgattcat tctatacgat tgacattgct taaagttgcc ggaaaactcg
     5881 ttaaaacagg tagacaagtc tatctcaaac tgtcgagtta tcatgtgtat caaactgaat
     5941 tttataaggt ttttgaacgc ctacggcgat ctaggcaatg gatttaggct agttatcgta
     6001 aaaattttta acctattttt ccaaggggtc agtctgccct taaatagaca aataattttt
     6061 attatagcct tcttccgtat atatcctgtt cgaaaacaca ttattttgga agaatgtatc
     6121 tgcctaattt aaatataggc actttgttca aaaaaatagc ttaaatttag agctatgaat
     6181 tattcaggtg gtatatatct ttcctccatt tttacaaaat tcccaagcaa cattatatgc
     6241 atctatcgca ttaaatataa ttaaatttaa tcttttctta ctcacgatac atttatttgg
     6301 tatgctttca aataattttt taaaaacatg tttattgtta ttatatcctg gcatacgatc
     6361 taaatactgt accgtacccc ccacgtcaga aagcacatat gctgttttgt agttagaata
     6421 gcattcttta taataatcta tgctattctt caaggcatac gaagcacaat gaaaaaccgt
     6481 aattttatgt ggtctttttc ccattcccat taataatact ttcgcattat actcttttgt
     6541 tgctatacta tatggagaat tttcgtcaaa ctgataaaga ctgtttttgt gtttgcttaa
     6601 gatttcttca gcatgttttc cataaccaca cattgaaaat ttagggaaac aacttctcat
     6661 agttccagga taattcctta aagactcact caatactcca gcaacagatt ttgttttgtt
     6721 taaatcaaac gaactattat cttctccata tgccggcatg agtaatgttc cctcttcttt
     6781 gccaatttta tctaatagca accttattac atcctctgga cacatatcaa gcatatacaa
     6841 tgctctccat gatgcatgta caattaaaac atcccctttt tttacaccaa tttcgtccaa
     6901 atattgtcct aactcttcct tgaaattttt ctatatggaa ttttcttttc aaaatgataa
     6961 tagtgtcttt ctattccatc tgctacagtc tcattttctt taattctaag aatacttcgt
     7021 aatacttttt ttatcatcat actttccctt catgccctca ttttccacta ctacatcacg
     7081 ttgataacta aacctcggaa ctaaataaat gattatgcag cctaacaaaa agccatgtgt
     7141 aagcataacc gtaaaaaagg atgaacttaa cagtgtatac gctgaaacaa taattattcc
     7201 catacagatt ccacttgtta agccttcaca aactctatct aacaatttaa gcgcacatac
     7261 aatcaaaatt ggtaaaataa caactcctac tgctccaaaa tttgaatatg catctgcaaa
     7321 caatccatta ttagcataac tattatttac ataatattgt ccacctacaa gagttggtat
     7381 atcttttgta tatggtgatg aaaagcctaa tcttccgaca atactctgtc taaaatagtc
     7441 ttttacatta ttgctaaaaa aatcaaagta tgcatattgc aaatatgctg ttaccaaaaa
     7501 taatcttcgt gcgactaaat ttataaagtt ttctgttcct atacaaatct tttctataaa
     7561 tccgatgact gtaactccca tcataattgc tggaatcatt cttgcggtta aattatgtat
     7621 aaaatagtat cccaatattg tagcaattaa aacaaatatc gttgattttc ttccatctgc
     7681 aaaaaaagcc aataactggc atattgttgc tgccaatgct aagattcttt ttttcttgtt
     7741 taatccataa acaatgataa ccggaaacac gataatacat gctgaaaata aatattgcaa
     7801 aattgttggt aacttatcac ttttaaacgc tgctcttgtt ccataaacgt catataaatt
     7861 aaatgaaaca tgaatcccag tatacgcaac acatatgagt aataatactg ctgcaaagaa
     7921 aagaaacact gcatagatca gtaatgaaga cctttttatt gtatttttac ctaaaactat
     7981 ttttcgtcct ttgtgtttat aaaaagctac cataaagaac cagtataaga catttaaaac
     8041 tgcaaattcc aaattatgac ctaagaatgc tactattgtt gttgttggaa cagccgaaca
     8101 tatcaatata attgataaga caactgctcc atttttctct acatcaatta ttgtaaacat
     8161 catcaatata tagcatatga ccataatcca agaaaccata taataaaacg tcgattgttc
     8221 tgcagtcatg cctatataag aatataacgg tgaaacaata ttacagtaca tataatccaa
     8281 taacactcta aaggcaataa tatatatgct ctgtaaaacc ttatttggtt ttaataaaag
     8341 attttttata tttgttaaca tgaattttta ttctccataa tttcttgata tatttttaca
     8401 tattcctttg ataagttgtc ccatctaaat tttttacttg cttgcataca tgcttcagga
     8461 aattcattat agcgttttaa tatttttatt atattctcgc ataattcctc aggatcatgt
     8521 gcatcaacat gatagccaac aacaccttcc gcaaattgcc catcaaatcc ctgcccttta
     8581 gaatatataa ctggcaaacc ttggctcatc gcctcagcat atactaaccc gaatgattcc
     8641 gtaaaagatg gcattacaaa aatgtctgca tttctataag catcaatcaa ttgttccttt
     8701 ggctttgcat cttcataaca agtcgagtct tctgaaataa tcttctttaa ttccgtttta
     8761 tcatctactt ttccaataac taatagttca ctatttattc cttttttctt taatatcttc
     8821 attgctttct gcgttgtagg tatattttta tttctatcaa tccttcccgc ataaactaat
     8881 ctaatgaaat cttttttttc agattttttt atcgtatctt ctggctgatt ttcaaaccaa
     8941 aaatcatcta ttccatttgg tattacaact gttttctcat atatctcatt tctatatttt
     9001 tctggtacat agttgtttaa aacggtttcc ctatatacat ttgacagaaa aaaaactttt
     9061 tgagcttttc gcattatttc aattccacga ttcctgagca aaaatctctt agcaaaaaaa
     9121 tcatttacat cagtatttcg tacagcaacc acatatggaa taccatattt gtctgataat
     9181 ttcatagccg aatttccatc agtaaataac gtatacgcat gaatacaatc atatttttcc
     9241 aaatcaaaat cttcttctat ttttttcaaa atttttgctt gtttaaaatc aaaattaatt
     9301 ctatcccact tattaaagca ttcttctatt tttacatatt cttttgtatt aactatactt
     9361 cgttttttat catatgtagc tacaaataca tcatttgaaa atcctgcagc atttaatttt
     9421 tcaatcaaac actgatgcaa tgatgacaat atgtagttac aattaatgtg caggattttc
     9481 atacttataa ctcctctgct aaaattataa tctccaataa ttcattccag actttttcaa
     9541 atcagatact gaatacaaac cttttacatc aagtaacact ttttcattgt ccggacaatc
     9601 tttaaacaat gctttaattt catccagcga catttttttg aactcgttat gtgcaacagc
     9661 aacaataata caatctgcat ttttcgcatt ctctaattca cttaatttta caccatattc
     9721 acgaaatgca tctttttcgt cagcccatgg atcaacaact actggatcaa tatcatatgt
     9781 ttttaattgc tttataatat cctctacctt gctatttctc gtatcagggc aattttcctt
     9841 aaatgttaaa cctaagatta caacttttga tttttttggt gactgtcctg ctacaaccat
     9901 cgttttcact gctgtgtctg ctatataagc tcccatacta tcgttaacaa ttcttccatt
     9961 aaggattatt tgactatggt atcccaactt ttcagcttca tatgtaaagt agtatggatc
    10021 aacacctata caatgtcctc caaccaatcc cggtctaaat cccaatgcat tccattttgt
    10081 attcatacca tcaacaactt catttgtatc aatacccatg cgatcaaaaa ccattgctaa
    10141 ttcattcata aatgcaatat ttatatctcg ctgactattt tcaactactt ttaccgcttc
    10201 tgcagttttt atattagata caggataagt gccaacttca attactaaat catatacatt
    10261 cttgatttca tttaaagaat cttcatccat tccagataca attttatgaa tattttccag
    10321 tctatgcacc ttatcgcccg gattaatacg ttccggagaa tatccaactt taaaatcttt
    10381 tccacatttt aagccagatt tttcttccaa aatcggaata cacacatctt ctgtgcagcc
    10441 aggatacact gttgactcgt aaacaataat ggaacctttg gtcaaatttc ttcctactat
    10501 ttcagacgca ccaattacag gtgtcaaatc tggtgtatga tctgtattaa caggagttgg
    10561 aactgcaata ataataaatt tcgcttcgct gattttcttt tcatcagaag tgaattccac
    10621 agtagtcaat tttactcctt catttccaac ttcatttgta ggatcaattc ctgatttata
    10681 taattcaatt ttcttttcat tcaaatcata acctattacc tttgcttttt tcgcaaaagc
    10741 caccgcaata ggcattccca cataaccaag tccaactata gctatttttt cttctccatt
    10801 aattattttt tcataaagtg acatttttct tctcctcatc ttcgttattt aataattttt
    10861 tcatactttc tttttttcat aactccaaaa aacatattcc aacacgcata ccataaagca
    10921 actggcgcta tcttttcatc acacatatgg aataagtcgt aatggctttt cagttttttg
    10981 cttaacttat catgtgatat agattttttt cttattctat attcagccaa gttttccttt
    11041 aatagaaagc aatcagcttt tttacacaat ttcaataaaa tcgcatagtc attattcttt
    11101 ttaatatcct taatctgaac caatcccatt gcttttgcac tgtacataaa tgttagacat
    11161 cctggatatc catagttata catttttcgc ttcgttacaa ttggaggacc agaaacatat
    11221 atattcaatg gctgtgagtc ctcatcaatc tttacatatt catgatatga gaaaatcaga
    11281 ttatgttttt tcataaattt aagttgatgt tctaactttt taggtttcca taaatcatca
    11341 gaatctaaaa atgcaatcca ttcacctttt gcttctctta atgctctatt ccttgtaaga
    11401 gctgccccac tatttttttc attcttcaaa tatttaattc ttttatctgc aaaagttcct
    11461 acaacttcat ctgtattatc agtagaacaa tcatctacta taattaattc ccatttttca
    11521 tatgtctgat tgattactga ctgaattgat tcagctataa agtttccagt attccacgag
    11581 ggcattatta ctgatactaa tcctttttcc atatacactc cctacctaat cactgcaaaa
    11641 actgttttta tcattgttcc tatatcattt ataaaactaa atttcctaat actttcaaga
    11701 ttgtatttca tcttctccgg aagaaccttt tctacatata ctttgtccac atcatctgct
    11761 ttatccaata actctgcttc atctttatat tttatactcg cctcagatgt aactcccgcc
    11821 ggtaataaca acgtagcata catttctgat gtatacttct ttacataatg cgtactttcc
    11881 ggtctagttc ctacaagcgt catatcgcct aaaataatat ttattaactg cggcaattca
    11941 tccaaacgat atttacgaag tttttcgcct acctttgtaa ctctactgtc attggatacg
    12001 gtgacttggg tgccaatttt atcagcatta gcaaccattg ttcggaattt aaatatcttg
    12061 aattttctcc catattgtgt aacacgctcc tgacgataaa acactcctcc tttagaatct
    12121 cttacaatca taattgagat agtaattaat acaggagaaa atgttacgag cataacagat
    12181 gcagcaaata catcaaatcc tctttttaaa gccaaactaa tttttttctt ttttaaactg
    12241 tcataatatg gacgaacttc ctccgtccgc ataaattttg gtaatttctc ccattctcgt
    12301 aacacctgta tctcccattt ctgctattac atttaaatat attctttcac aattctacta
    12361 aactgcttga tcacataatc cacttcttca tctgtaagtt ttgtgtgaag cggtaaagta
    12421 atctcatttt caaatctctt atatgcatta ggataatctt taatatcaaa tcccatattt
    12481 ttgtatgcag tcatcattgg caacggctta taatgcacat tgcaggcaac acccacttct
    12541 gccattttca caataatctc atgcctctgc tccaacgtaa ttcccggaac tcttgttata
    12601 tacaaatgac cagatgactg atggttttcc gtatagtgat ttaatacctc tattccgatt
    12661 ggcttaaatg ctgaatcata tttccctata atttcttttc gacgttcaag caagccttta
    12721 tatctcttca tctgtgccag tccgattcca gctacaacat cagtcatatt acatttaaac
    12781 caaggtccaa tgatgtcata ctcccatgct ccaagctgcg ttttcgcaag tgcatctttt
    12841 gactgaccat gaagtgataa caactgatac tgatgataaa tttcttcgtt atcaattccc
    12901 tcaatatctt tccaaacagc agcaccacct tctgcagttg taaaattctt aactgcatgg
    12961 aagctgaaat ttgaaaaatc tgcaatactt ccaactggtt tctctttcca ggttgcacca
    13021 aaagcatgtg ctgcatccgc cataacaatc actctgccga tgctttcctg tattttattt
    13081 accggatgga ataagccttt cttacgttct acaatagaaa aaatcctatc ataatcacat
    13141 ggtactccac cgagatctac cggaataatt acttttgttt tttcagtaat cgcattctcc
    13201 aactgctcat aatccatttc aagagaatct ctctgcgtat caattaaaat aagttttgcc
    13261 cctacatggc atactacaga cgcacttgca gtatatgtat acgcacatgt aatgacttca
    13321 tcgccctctt taattcctaa taaacgcaag gtcatttctg cacatgctgt ctgtgaatta
    13381 agacaaacag cacgatttac tccacataaa tctgcaacct ctcgctctaa ttcttttgtc
    13441 ttaggcccag ttgtaatcca tccggatctt aatgcttctg aaacctgttc aatttctttt
    13501 tctgttatat ctggcggtga aaatggtatc tgacgttttt ctaatttatt catttatatt
    13561 ttcctcctac ttatttgaag catttgcaaa agtgacgatc tctttagcca attgttcgtc
    13621 gtcttgcggc aggctcttga caaaatccat cactgtttgc atggagtaac ctttgatgtt
    13681 tcccacaaag atcttctcgt aaactgcttc atcgtttctt tccttatcta acaatagttc
    13741 ttcatatagt ttttcccctg gacggatacc tgtttcaatg atttcgatct cgtcttcgct
    13801 gtatccgctc aatcggatca tgtttttggc tagatctacg attttcactg gttcgctcat
    13861 gtctaaaata aagatttcgc cgccttttgc taatgcacca gattgaataa ctaaacgact
    13921 agcttcagga atcgtcatga aatatcgggt catacgaaag tctgttacgg tgactggccc
    13981 tccttgagca atttgttctc tgaatactgg gatcacacta ccacgagaac caaggacatt
    14041 cccaaatctg acagctgaga atttcgtaca gctttcttca ttcaacccag tcacgatcat
    14101 ttcagcaatt cgttttgttg aacccatgac atttggagga ttgttagctt tatctgtcga
    14161 taccatgacg aagtttttca ctcttgctgc tttagctgct tctgctacat tttttgtacc
    14221 gaaaatattg tttttgactg cttcttttgg attatattcc atcagtggta catgtttatg
    14281 agcagctgca tggtagacta tatctggatg atattgttcc atgatctcat tgattttctc
    14341 gcgatcttgg atatctgcga tgatcggtac gatttctgta ggacaatttt gatgatgcgt
    14401 gcgtaactca cggtcaatca agtagatcga gttctcccca tgtcctaaaa gcaatagctt
    14461 tgcaggattg aattgaatga tttgccgaca gatttctgaa ccgatcgatc ctcctgcgcc
    14521 ggtgacaaga atgactttgt ctgtgatctg atcttttatg gattcaatat ccagctttac
    14581 ctcatctctt cccagtaaat ccactacgtc aattgttttt aattttgata cgctaatttt
    14641 tcctgaagca agttcttcta tagaaggcat cgtgttgact ttcacacgag cggattcgac
    14701 taactcgaag attcttcgta atttttttct tgataaggaa gggatagcaa tcgttaccat
    14761 ttgaatgtca tatttttcaa tcaattccgg aatatctttt aatgcaccta atactttttt
    14821 tcctgaaaga tacgtatttc ttttattcgg atcatcatct acaaacccga caacgtggat
    14881 atcttgagcc gttttggatc caagaaagct gttgtataaa atacgcccac cttcgccagc
    14941 accaacgatc aaggtatttt tagcagaatc tgctgataca tatctcatat tttttgtttc
    15001 gacatagata cgccagatca atcggctacc aataatcaat aatagcgaaa gcaggtaagc
    15061 aaaaatgact aatcgtaaac tgtatcgccg atttatgaac agcaatatga tgatacttga
    15121 gacagcactt gcagatagtg agccaaaaat cgcaatgatt tctcttaaat ttgtatagcg
    15181 attgatccga gtgaatactt tgaataaact accatagaat aaataaaaac caatcgacaa
    15241 accgatagag attaaaataa ggtccattgg tatagctaca aacggcttca tgaatttagc
    15301 tgctgcaata ttcgcagcaa tcaacaacaa gctgtccaca aataccaatg tggcaatttt
    15361 tcttcttctt gttaatacaa acaccctaca ttccccctaa aataaaccaa acttcttctt
    15421 ttttacctct tcatagattg gataactgat ttcatccccg ttgatcagat cctttgcgac
    15481 ttgtttcatc tgtactactt tttccttacc aaagtccttg gcaatctgct tatagcactc
    15541 ttttaagtaa aaggtgcgct tagagacccc atgagcatca gaagcagcca tctgcacgag
    15601 attatgtttg accatcgttt tggctgtttt ctggatgtct ttgccaaata ccccaacata
    15661 acttggcgca gtgagttggg ccaaacatcc catatctagg aacggaagca aatgatttgg
    15721 gtcttctcga aaatgcgcgt tgcgttctgg atggacgatc actggtgtct ttcctagttc
    15781 taacaactca aaaaaaagct gttctgcata taacggcact tccattgtcg gaaattcgat
    15841 cagaagatat gtatcatcta aatctgtaaa taaaatttca tctcgatgga tgtcttcgat
    15901 gagcgttccg gtaatacgta cttcttgtcc ttccaataac gtcaaaggta actgtctttt
    15961 ctctaattcg gcttgtaacg aagcgactag agaaatgact tgtgattttt cattgctgta
    16021 ttttccatta ttgtggtgag gtgtgcataa aatatgcgtg atgccttgct gaatcgcttt
    16081 ttcagccatt gcaatacttg cttctaagtt ttctgccccg tcatcgattc ctggtaggat
    16141 atggcaatgt agatcgatca tttctcttgt ctcctctctt cttatttctt aatttccgta
    16201 ataataacca gcatctttgc tgtgttctgc tccgttatat acgacaccta agactcgtgc
    16261 ttgtaccatc tctagcagtt cttttgcttt agtcaaggat tctttacgtg atgtattttc
    16321 acgtacgacc aataaagtgc catccgtttt tgaagacatg atctgtgcat ctgttacggc
    16381 tactactggt ggcatatcga atatcaccac atcaaataac tgtcttgctt cggctagaat
    16441 ctgatccatt cttggcgagc ttaacaattc tgatgggttg ggtggcttag gcccactagg
    16501 caaaatcgac aagttctcga ctggcgttcg ttggattaca tctactacac ttcctgatga
    16561 actaagtgct gtacttaatc cactggcatt gttcagctgg aatgtcttat agacgacggg
    16621 tttacgtaaa tctgcatcga ctaatagaac tcgttgacct gatttagcaa aaacgactgc
    16681 aatgtttgct gaagtggtgg atttcccttc tctaggacct gatgaagtca cgacaatcgt
    16741 tttgatttgc tgaccagctg cggaggcaaa ctggatattc gtccggatcg tacgatattg
    16801 ttcggcgatt ggggaagatg ggtttgttaa tgtgatcaaa ctgactgcat gagtttgtgt
    16861 tgctttttgt ttctgtgttc gtgccatttt ttttcctcct agactcttga gcggcttctg
    16921 cgtgatgggg ctgttttatc gtttgtagct tttggtcgtg caggagctga tcctttttta
    16981 cggataccag cattcaattc tttcgctgac atttctggaa ctgttcctaa gattgggaat
    17041 cctaatgtat ccgtcacata tttttcatct ttcactgtcc gatcaagtaa ttcgaataag
    17101 aatgccagac ctacgcctac catcattcct aacactaagc caatcactag attcaattta
    17161 ttatttggcg aaacaggtgt cgtattggca actgcacctg aaataatcga gattttatcg
    17221 acattcatca cgtcttttgc gttctcttgg aaaacttgtg aagttgtatt agcgatctgt
    17281 tgagcagtca ctgcattcgt gcttattgcc tggatcgaga acatttgtga attttgtgac
    17341 tgattgacgg agatcgcatc tttgatttgt ccagcagtca ttttcacatt gtcttctgtt
    17401 tctaaacgat ctttgacttc atttagtact aaatcactga cgatcatatc tttatacgta
    17461 ttgatcattt gcaggttcgt attgacatca ttgacatttg ctgtgtctga ttgtggcagt
    17521 gtcacgatca attgtgcttg tgaactgtat tttggtgtca agatgaaaaa tgttacgatt
    17581 cctgagatgg ccaagccagc aaacattgag atgatgattg ttgccaagtg tttgcgtaat
    17641 gtttcaaaaa tttctttcaa gctgattgtc tcttccattt tttctccact ttcctattta
    17701 ttctctagat tcatttgatc ttttaattct tttttcacac gatcaagttc ttgatcactg
    17761 actttttgat aggagatatt atcttgcatg aagccttctc cttgcagctg atcttgcttg
    17821 atcgtaccaa atgcctcccg ataatctaaa gcgatttttt tcatttcatc aaagctcatg
    17881 tctgttttca tattcgactc tagagcattc aaaacggatt gatagttgct gacaccattt
    17941 agactcaata ctttttttgc tacgccttct acgatctttc tttgacgctc ttgtctacca
    18001 tagtcaccat tgggatcttc ataacgcata cgagaataag ataatgcctg atccccgttc
    18061 aatgtgattt taccgattgt aaagtcatag ccatcttgtg agaaagtgag gtcattattg
    18121 acttcgatac caccaactgc atcaacgagt tcctttagcc ctttcatatt gatagagaca
    18181 tagtgatcga ttgggatgtc tacatatttt tgtacggtat ccatcgccat ggcagctccg
    18241 ccgaatgcgt aagcgtggtt gatcttgtct tttgtgtcac gacctacgat gtcgacatac
    18301 gtatctcgag gaatgctgac gatcgttgtc tgtttgtctt caggactgac tgtggcaacc
    18361 atcatcgtat ctgatcttcc ttgatcgacc cgacctaaat ctccagtatc aatccccatc
    18421 aatagaacag agaaagattc ttgtttgtcc agatcaactg gtttcgttcg ttctccttgg
    18481 gttcgttcca ctgattcgta agttttctca atagaatgtg agagatccag atataatttt
    18541 gctcctactc ctacaattgc taatactaat acgacaagaa ctgctaaaat aatcagaaat
    18601 atttttttcc ctcttgattt tcttcttcgt cttgccatag tttcctccgt ttgctaacct
    18661 tgcttgaatc aacgttttaa ctgtcaatag cttatattgc tatagcccat ttgctcagca
    18721 acagtttgtg cttttgttgc gtatgctacg cttatttttt cattccggat cttctctatt
    18781 tgactgagcg ccgtcaaata ttgatcgtaa gtagcggtgg cagtcacttg accatccttt
    18841 aacatcgtat cgaataactg atctagttgc gtgacttgcg cgatctgagt atcagcgaat
    18901 cctagatatt gcataacaac cgttttccaa ggactatctt taaaaaagct catattttca
    18961 cgtacacgag cgacatctgc actggtcacc ttttctttga tgatgacatc gtctacagct
    19021 tgctgccagt ttgatacatt tttagtaaat aaatcgttga cagctgtctg gatcttcagt
    19081 tttgattctg cactatctaa ctgtgtcacg attgctttct tctcatcttg gattgcaaca
    19141 gcttctttag gcaattctga ttctttgatg tcgtaatcat ctgctgaaag tttgacagag
    19201 gatagcttat tggacaaact tgtgattgtt tcttctgtta tttcagggtt tagaaaaatt
    19261 ttttcttttg tactatacaa cttgtctact tcttttgata aagaagtcaa ggtatctttt
    19321 tgattggtga tggcagattc tgcataagtg atccgttgct gatgttgccg ctgattattg
    19381 atatggtatc caaacaaaca gatcgcaata gcacctgcac ctaccgccac agcagtcaat
    19441 gacttgacga atggcttttc catagattca ctcctaagta ttgatgatag ataaattaag
    19501 atataattca cttacataaa aatgacaact ttttatttta aaaaacataa ttaaactaat
    19561 atattcacag aa
//
LOCUS       DAZABD010000048        18967 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000048 DAZABD010000000
VERSION     DAZABD010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18967
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.048"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2303)
                     /locus_tag="ACHALG_001903"
     CDS             complement(<1..2303)
                     /locus_tag="ACHALG_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFN2587285.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTGLTTGEDGKLAIDGLAPGAYQLVET"
     gene            complement(2975..3243)
                     /locus_tag="ACHALG_001904"
                     /pseudo
     CDS             complement(2975..3243)
                     /locus_tag="ACHALG_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            complement(4211..4972)
                     /locus_tag="ACHALG_001905"
     CDS             complement(4211..4972)
                     /locus_tag="ACHALG_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFN2587286.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
     gene            5072..6793
                     /locus_tag="ACHALG_001906"
     CDS             5072..6793
                     /locus_tag="ACHALG_001906"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN2587287.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            6808..8592
                     /locus_tag="ACHALG_001907"
     CDS             6808..8592
                     /locus_tag="ACHALG_001907"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN2587288.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(8973..10526)
                     /locus_tag="ACHALG_001908"
     CDS             complement(8973..10526)
                     /locus_tag="ACHALG_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HFN2587289.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10874..13288)
                     /gene="leuS"
                     /locus_tag="ACHALG_001909"
     CDS             complement(10874..13288)
                     /gene="leuS"
                     /locus_tag="ACHALG_001909"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFN2587290.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     misc_binding    complement(13439..13645)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(13715..15733)
                     /locus_tag="ACHALG_001910"
     CDS             complement(13715..15733)
                     /locus_tag="ACHALG_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFN2587291.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     gene            16103..16759
                     /locus_tag="ACHALG_001911"
     CDS             16103..16759
                     /locus_tag="ACHALG_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFN2587292.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            16759..17715
                     /locus_tag="ACHALG_001912"
     CDS             16759..17715
                     /locus_tag="ACHALG_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFN2587293.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            17715..18281
                     /locus_tag="ACHALG_001913"
     CDS             17715..18281
                     /locus_tag="ACHALG_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFN2587294.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            18717..18947
                     /locus_tag="ACHALG_001914"
     CDS             18717..18947
                     /locus_tag="ACHALG_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587295.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
BASE COUNT         5566 a         3805 c         3365 g         6231 t
ORIGIN      
        1 gtttctacca gctgatatgc tcctggtgct aaaccgtcaa ttgccagttt tccatcttct
       61 cctgtagtta aaccggtttg tagcgtttct ccttctcggt tctgtaactc aaaaacagct
      121 ccttgaagga cttcaccact gccttcagca tgtttaatta gttcaacacc acccgtatca
      181 aggatgtttt caatggttaa cgtcacttct tctatggagt attcatttat aaaaacatca
      241 tgtaccgtcg catctaaaag atacccttcc ggtgcttctg tttctcggac ttgatagcta
      301 cctacaggaa gcggttcact taaaaattct ccgttttctg ccgttatacc ttcttgtatc
      361 acttgtgaat cgcttgataa aatttcaaat tttgctcctg ccagcctttg gccattctca
      421 tcagttttaa ttactttaat acgcccatcg attttccttg ttcttacagt actcgtattc
      481 aatcgattgt cactattcac aagcgttgca aagtttaaaa tttgatcgcc gaccgcatac
      541 gcctctacat tgaccttaaa agagagggta aaacttgtcg gaccaccaat cgtattattc
      601 gcactgaaac gaactattcc attattgact tcggcatcaa tgaaagaggg aatatctgat
      661 agaaatgaca caaattccat gccttcttcc aaaacatctt caataacaag cgtatttctt
      721 gttccagaaa acatcgagac atgcagagta tacgttagtt cattttcatt gagattgata
      781 acatttttgt ccacactttt tcttaaagag attgccggaa gattactttc agggattctc
      841 aagcggtagt ctcggctcat tacctgatta ttgtaggtca tactcgctcg attccgagaa
      901 aacccatcct cttgtcttcc ttcattgaac aagtcgacat cggtcacttc aagagcatat
      961 tcaataaaat acgattgacc ctctgctaga ttaccaaagt ccacaacaag tgtattcgtt
     1021 tccttattaa aagatacttt gtccacaaat tcctcggaaa cataatgagt tctaacgttt
     1081 gcttcacctg gattcgttac ttctgcgatc cgaaccccac ctgaaatagg ttctctattt
     1141 ccagtggaac tccatgtagt cggattcggt gccaaagttg ttccttgcgg tagtacatcg
     1201 gtaaccacta caccgtcttt tcgtgatcct tcataattta cctgcaaaat aaatttatta
     1261 ctgttggtca cgtcttgccc gtcaatcagc agattcccaa gatttccttg gctgtcgatt
     1321 tctctagagc catagtataa tttcgaaaaa atgggtcttg atgaaggaat aggcgtaatt
     1381 gaaaattccg tgtttgctgc tcgctcagca taagtaagac tcacttgaaa gtcattattt
     1441 ggctcagtac cgcctgattg aaagaaagca acttcaaacg tgatactgaa tgtatacgac
     1501 tgagcggctt catttaatcc ttcataatca aaggtaaatg gaaaaacaac gtctgtgcct
     1561 tcttggtaaa atgccagtgg ctttaaaggt gaatcatcag cgacctccac tctaatgaac
     1621 tcaattaagc caatcgatgc tctaggaact cgtacgacca tctccccata ttcttcaaga
     1681 atatcgccag cgcctccttg aatgtatccc tttaacgttt gtgtatgcct gctcggcaaa
     1741 gtggggcgct cagcagagat gccgatgtca taattcaaga gctcctcttg tctttcaatc
     1801 tgagattcct gcgttataga tggctcagat gatctggctt cattaaaaga ttcacttaag
     1861 ctttcttctg gtatattaaa agattcactt gtgctttctt ctggtacatt aaaagactca
     1921 cttgtgcttt cttctggtac actgatttct attgagaccg tatttgattg aatatcaccg
     1981 cttcttgcgg tcaattcgta tttacctgaa tgctgggaag aataagtaat cagacgttcg
     2041 taatcattga gttgcttcac ttccccttca atcaaatcct cactcttatc aatgaccaat
     2101 gcatttagca ggtctggtgt tcctctgact cgcaactgga actgctcacc caaagcagcc
     2161 tcctcttgat caacttcgat cgtcagcgaa cgctctggct gttctgctaa agactcagct
     2221 agaaccgtta tgttcccaaa ttgattgatc acaataagca taactagaaa catacttaac
     2281 agtttatgaa atttgttcct cactattact cccccttata attttgacta aacaacaagg
     2341 tacccgataa ttttctaaaa agattcccca actcaaaatg gctgcgttac ctccctcgta
     2401 ttttcggttc aactcaagcg ttgcaatgta agctgtaaac aaagctaatg attgccgatt
     2461 gtcttctgtt gattgattcg atagctttgt atgactttta gttgtccgtg tggcttaacc
     2521 ataattatga tttcacgatt cctcctgcca gtgccttgca caagctggag cgtaacccta
     2581 atagttattt tgttaatgta cttatatact tttatacttt tccattagaa acaatcacta
     2641 aatcgttgat accaaggtca gactctagaa tttcatcagt gagtccctgt attggttaac
     2701 cttcatcaat cccaaaaaaa tatatcaatg cttagcatca aaatgaatat atagtttaga
     2761 atacttggga ttcatcgata actgatcgct tatttggcca tattatttgg cacttggttc
     2821 aactaaacgc atttaggatg ttccttcatt ctcgtcatgg atgttctcaa accgtcatta
     2881 ctaataaact gtccaccgaa tcgatggttc atttcttgca ttttccacgt ccaattactc
     2941 tttttgctct ttatttgggc gtagtttcac tttcttaact tgtatcgtct tccatcaact
     3001 ccttaatcat cttcttagct ttattggctc ggtttccttg taaaaggtat ctacaaatag
     3061 tgacaatctg tattaaatct tcatcatttt ctgtaatgtc aataatttct atattctgta
     3121 ccatatattg caaagggttt cactaagttc ataaccgaag cgaattaaac aacttttgta
     3181 tagaataact accttttcta tttttccatt ggtaattcta tctattaatt ggtttaatcc
     3241 ttttgttgta gttgattcca ctaccaagat gattctctcg aattttaaag cttgaagatg
     3301 tattgctatt tttgctaact ggcgtatttg caactatttt tcccttctat tcttccttca
     3361 ttgattcccc tctaaaatta ctatccataa aaaattgcat aaatgacaat agtatttata
     3421 ttagtcatac tgctttttaa cacagcaaat tccagaaaaa cattcgttac atatttgtga
     3481 atttttccaa aaaataacat ttttataaaa cgacgaaaaa taaatcacta ccagtcaact
     3541 tctataatat taccaaataa caaactaact atccatcaat aacgtgtttt attttttaaa
     3601 atttctataa aaaaaacaat cattcttaaa aagaatatat aaaaataaca ataacctttg
     3661 tgatttttct tgtttgaaga atgttagaag aaaaatctct aaatacttat aattatttta
     3721 taagcaaaaa aatgctatcg gtagtatcac cgatagctga ggaaagtcac agtagggact
     3781 atttgattaa aaaatatgtt tattcaatcc atctatttat ttttgcctta ttaacatatt
     3841 gtaataatgt aagcaacaat gtattttaag tgtaatgctt ttatgcacct atttaattta
     3901 tcattctcat ctgatacaat ctcactcata acaatatgaa tttttcttct cattagtctc
     3961 agttcaccat gaattataca taacacccaa tacaattatt tttccatact tttttaagag
     4021 gacaattaca tcgtatatat cgctaagaat tgcaaccatt ccatttttaa agacatattt
     4081 cctctaaaaa actaaaattc tatctatcca cgactaataa tcccccacat taaacccgta
     4141 atcgaaccag aaaatttttc tttataacta aaacaaccat cattttcgag ttatcttaaa
     4201 cacatatttc ctattttttc atttctatcc atgcatgata atattcagtt ctgatattgt
     4261 tttcatccgt attcgtcaat agatactggc cgatcacttc tcctgttttc catccttttt
     4321 catacattct ttctctgatt tctaaaatat tattacgttc cgcatggttc atctcggatt
     4381 tcaataagaa tcgacgataa acagccgtat cttttttctc ccaaatcacg tcttgagtaa
     4441 ataaatgtac ttcactaggg tcgataaaaa atccatacat cgtctgtgaa ctggaagctg
     4501 gcatactcat catcccaaag cttgaatagt taggtagaaa ttttttgatt tccacaatat
     4561 cttcaaactc cgttgagatc atacagtcaa aagggatctc ttctacagga aattcttcct
     4621 cgtttgtatc gatcatcaat ttcaactgtt ttttttgctc ccttatctct ttttgttttt
     4681 gtttcatcac caccagttct ttatggattc ttctttctgt ttcatcaagt gtctcatata
     4741 actccgtcaa tgtttttcca tataaattct tcatctgttt gatcggtata tctaattttc
     4801 tataaaaaac gatatcataa agcataaaaa tatcatcgat atcaaacaat cgataatcat
     4861 tctcttcttg tctacttgaa cggattaatc cttgttcatc ccaatagcgg atcgttgatt
     4921 ttggcaaatc aaatagttcc gatacttggc caatcgtata catttttttc attttttctc
     4981 acctcttttt cagtttattc ttttttctat tgaccttcaa gttacttgaa ggtatagaat
     5041 cgaaaagtat caggaaaaaa ggaggaataa tatgtctatc ttaaaaacat tcttacagaa
     5101 aaacaaagga ctcgctctcc ttacctttgt gatgatctgt ttagaaatcg ccggtacatt
     5161 aggtgtacca aaattagtag caaagctgat cgacacaggt atagcaagtg gcgaagcctc
     5221 agttataaaa aaaattggag ttgaaatgct gcttgtagcg tttctaggaa caatcgctgc
     5281 agttgcctct agttactttt cagcattggc ttccgctagg tttggattcc aaacgcggga
     5341 gaaatatttc cgtaaattcc aacagctgtc aatgaaggat atcagtcgtt ttagttctgg
     5401 atctcttttg acaagaatga cgaatgatgt cgataatgtc cagcaaatga ttgttttatt
     5461 ttgccaaatg atcttaccag caccggttat ttgcgttttc accattctca tgatgtttcg
     5521 ttattctcta ttactaacat gggttacatt gatctctgtt gttttttatg tatggattgt
     5581 ttatcgactg atgaaacgag gggcaccttt atctttgagt atccagccaa aaatggatcg
     5641 gatcaccatc acattgagag aattctttac tgggattaat atgatccgtg cctttgataa
     5701 tcaatcatat gaagaaaagc gaacgaataa gagctttgct gactatgcta atcaaatgat
     5761 caaagtgaac cgaatatttg cttgggtcac tccaattgct tttttgctca tgggaatcgt
     5821 ttatgcttct attttgtggt ttggcggaaa tcttgtagca gaaggagcat tacaaatcgg
     5881 tgtcgtgact gctgtagtcg aatactctat gctgacactt gcttacttga tgatcgcagc
     5941 tatggtattg gtcattatcc ctaaatcgtt tgcctcatta cgacggatag aagaaatact
     6001 gcaagcagag atcgaaattg aggaagagac gatcaaaaca gtagctgaac caacctttga
     6061 tcagacaaat gcagctgctt ttgaacatgt cacatttaaa tatactgaaa tctctgatcc
     6121 agttctagaa gatattgatt tcacaatacc taaaggaaaa acaacagcga tcgttggcgg
     6181 gaccggatct ggaaaaagta gtttggcgaa acttctcttg agacttagtg accctacgat
     6241 aggaaaagta acccttggtg gtattcctct tactcaaatg actcaagaac agatccgctc
     6301 ccacatcagt tatgtccctc agaaggcttt cttattcagc gggacaattt taagcaactt
     6361 gcagatggga aatgaatatg cctcaacaga agagttgtct aaagcgatca agatcgctca
     6421 attagaagaa ctagtggaca cccttcctga cggattggac agctttgttg ctcaaggcgg
     6481 ttctaattat tcagggggcc agaaacagcg gatatgtatt gctcgagcgt tgatcaaacc
     6541 agcagagatc tatgtattcg atgatagttt ctccgcttta gactataaaa cagatgcagc
     6601 attaagagca gcccttcata aacacatgtc ggagaaaact ctcgtgattg ttgctcaacg
     6661 cctcagtaca atccaacaag cagataacat cattgtttta gatgaaggaa aaatcgttgg
     6721 acaaggtact catgaagaac tactgtctaa ttgccaaact tatcaggagt ttgcaaaatc
     6781 acaaggaata tagaaagaag ggatacgatg gaaaagaaaa aaacgttgaa cacgcgtgct
     6841 ttgatacgtt tttggaaaat gatcaaacct gaacataaat atttttacgg attgtttcta
     6901 tgcagtttat tcgggaacct attagtcgta gccatgccct tgatcatggg aatcgggatc
     6961 gatgatctct tatcagaaat aagaaaaaca ggtattggac agctttcttt cagtgatatc
     7021 aaacaagcat tgttgttccc tgtcttattg ctgatcggct gttcagtctt aagcagcatc
     7081 acttcattta ttcaggaacg gacaatggca gcgttaagcg aaaagatcac attgagggtg
     7141 cgaaaagaaa tcacaaaaaa attcaaaaca ttaccgatgt cgttttttga caatcatcaa
     7201 gtaggtgaca tcattagtcg gacaactacc ggtctcaatc aactttctca agtgttattg
     7261 acaggtatca atcaattttt tacttctgtc gtaacaatag ctgctgctgg tctgatgctg
     7321 ttttatatcg aaagcaagct tactttgctc gtcttattgc tgatcgttgg cagttcaatt
     7381 gttacaacta agattgcgaa caaaaacaaa cagatttctg aacagaatca gtatgaactg
     7441 ggtgttttaa ataatcgaat ggaagaaatc ttagctggta atctagtggt caaaacattc
     7501 aatcagcaag ctcatgcaga agctgctata cacgaagtaa ataaaaagca atacaacgct
     7561 tttaaacgtg ctcaatttat gaactgtgct atctatccgg ccattcgttt cattaatcaa
     7621 ctcgccttca tcataagtgc ggtattcggt gctatgcttg ttctatcagg tggtgtgacg
     7681 atcggcttct tacaagctta tcttcagtac atcaatcaga tttctgaacc aatttctacc
     7741 gcttcttatg tgatcaactc gatccaatcc gcaatggcag caatcgatag gatatttgag
     7801 atattagatg aaacagatga aattccagac cttccagatg caagactttt agaacaaccg
     7861 aaaggaaaaa tagctttcaa aaatgtacag tttggttaca catcagataa aatattgatg
     7921 aatcaagtcg atttttcagt aaagccaaaa caaacggtag cgatcgttgg tccaactggt
     7981 gcaggaaaaa cgactttagt caatctgctg atgcgttttt atgaactgaa taaaggaaag
     8041 atcgaattcg acggtcttga tattaccaag ctttcacgtg gagaactacg tcgttctttt
     8101 gggatggttt tacaaaatac ctggctgttt gaaggaacgg tagctgataa tatcgcctac
     8161 gggaaaatgg atgccacaag agaacaaatt atcgaagcgg ctgaaatcgc acaatgtgat
     8221 cactttattc gaacactgcc agacggatat gatacgatca tttctagcga aaacggcacg
     8281 ttatctcaag gacagcaaca attattgaca attgctcgag tcattctagc gaatcctcct
     8341 gtcgttattt tagatgaagc aacttctagc gtagatacac gaacagaagc attgattcag
     8401 caagcaatgg aagctgtgac aaaagatagg acaagttttg tgattgcaca ccgtttgtca
     8461 acgattgaaa atgcagacat gatccttgta atgaagaatg gcgatatcgt cgaaaaagga
     8521 acacattctg aactactcca atcaccaacg ctttatgcca gtctttataa tagtcaattt
     8581 cagatagcgt aacaaacatt atctaaaaag ctatcgatca tgtatgatta gtccatgatc
     8641 gatagctttt ttaaatgtac tagtggtgcc actagaaaca caaagaataa tactaatgat
     8701 actttttgga tagctccttc agtttcgttc ggtaagtagt gacaaaagac ttgtcacaat
     8761 ccctttttac gaataaacgg tgttcaagag acaacctatc ggcaataaga cacgatcttg
     8821 taaaatacga gaagctatac ttcaaaatcg cttcttgttg ctcttgtcaa ctccgaatca
     8881 cgagttacac tattcaacaa atatgagaag ctgttttttg aatttgtttc ttgtgactcg
     8941 gtgctgaacg tcaggctcac aatctctctt ctttaatctc cttgttctac ttgcgatagt
     9001 tgctgaacga attgccatgt ttccccgtta tcagttgaac ggaagaggca ggcaaacatt
     9061 ttccctgcat cgattggtcc ttttttcaac aaataaaatt ttgtctctaa cgtattcgcg
     9121 cttatttgag taacttcatt tggtgattga aaaagatcta tcccccctgt ctgatagttt
     9181 tccggaatcg acagaactga ttctttgaat gagcgtcctc cgttgtttgt ataaaacaaa
     9241 ctttcacgtg tcgaaacaaa acctaaagaa gttgtaacga agctgacatt ttgtactggc
     9301 tgattgatca ttgtacttcc actttttatc caagtgattc cgccatccat agtagtgtaa
     9361 atgtacaaag tttctgcaga catcccactg gctgatgtac agataaatag accatcgttg
     9421 ccataaaata ccgcttttcg ataacgtatc ggctgaactg attgggtgat aatagacgtt
     9481 tgccatgttt taccattatc tcttgaagtc agcagacgta catcatcccc agaagaatag
     9541 ataaaccatg agaaatcggc ggaaatatca tatgtgttgt ccatccaata accttcagga
     9601 atcagccccg ttgtcagggt gtattcgcca aaacgcaacc attgcgaatc aatcggcaca
     9661 aatacccaat tagcaccact atctgttgtc atatagagag agtgcgtctc tgtatcaagt
     9721 ttgagatcat ttatcttttg tgttgtcaaa tttacctttt gcttaggatc agctaacaac
     9781 ccttcatttg tcttttgtat atgcgtatcg ttagttgagt tcattgtcat gtgagaagca
     9841 gcatttatat gagaagacat ttttggctga gtgtatctcc cataatagat tgttccagct
     9901 aaaagaatga ggaaaaaacc agtgactaat gtactcatct tagttgtcag gtatccacca
     9961 ggcgaagact ggcttttttc ccatctgggt tcgttttcta gtaaaaaaat atggaatgtg
    10021 ttcattccga attttgattt gctttcgcct aagtagatag gtaaatcatc aagtgatctc
    10081 agcaataact ggcaatcact tttttgtatc cgaaaagctt gactcgtcga agtcttctca
    10141 aaataaagat agtcatcatc ttgagaagtt ggtttattat gtaaaaactg ttgtgctctt
    10201 tgactaagcc attgatcgaa atgacgcgac aattgatggt aacggagcca aataagtaga
    10261 agaaaaacaa taagcacaac aattgttatt ccgccaactc cccacgaaaa gtgatacaaa
    10321 ttatttaatt tttgcatccc ttcagctgaa cggactactg cactcatatg gcgatatagg
    10381 aacgatacag ctccgaaaaa aagcaaacca gagaaaaata ctaagcttaa aagcttgaac
    10441 attattcttt tcgctctttt tttcgctttc tcgataattt tttgtttagc tgataatact
    10501 tcgataataa atagttcttg atccatttgt ctctcctttt ctcatacttc attcattatg
    10561 cgtcaaaatc catacaatca ctagtcccac taataaaaaa tttatgaatt tggtatgtac
    10621 tctcaatttc aggtagttta tacatcaaaa aatactaaga ttaatagaca aaaatttgat
    10681 gattacagag atctcttaca agaccattca gaagaaaaag acattgaacg tgtttgagaa
    10741 catgaaaatc aaaacctttt tgatcaatca attaatcaat ttgaaataac aaaaaatctg
    10801 agacagaatg aagttcattc tggcccagat ttgattttgc acaactcatc agtccttatt
    10861 gacaaagagc caattaatta gcaacgatat tgactaactt gtttggtacc gcaataactt
    10921 ttctgactgt tttgccttca atatattctt gcactatttc attggctaat gctgcttttt
    10981 ctagctcatc ttttcctaag tctcggggaa cattgctttt cgcacgaact ttaccgttta
    11041 cttggaaaac aacttctact tcgtcttcaa caagtgctgc ttcatcgtat gttggccatg
    11101 gtgcgtaaga aataccacca tcatttccaa ggattgccca tagttcttca cccatgtgag
    11161 gagcaatcgg tgcgagtagt tgaacaaatc cttcgatata ttcataaggc agagcgtcca
    11221 ctttataggc ttcattgata aagaccatca attgggagat tgctgtattg aaatgaagat
    11281 tttcatagtc ttctgtcact tttttcactg tttgatgata cactttatca agcttgccat
    11341 cattcaatgt agtgatacgg tcacgcattt tattgttctc atcaacgatc aaacgccata
    11401 cacgatccaa gaatttacgg cttccttcta agccattttc gctccaagca atcgaagcat
    11461 ctaatggccc catgaacatt tcgtacatgc gcagtgtatc tgcaccgtaa gtttcgacca
    11521 catcatcagg attgactaca ttaccacgag attttgacat tttttcgttg ttttcgccta
    11581 agatcattcc ttgattgtat agtttttgga atggttcttt tgtaggaaca acaccgatat
    11641 cgtataagaa tttatgccag aatcgtgcat atagtaaatg aagtaccgca tgttctgctc
    11701 caccaatgta gatgtcaact ggtagccaac gcttcaactt ttcataatct gctagttctt
    11761 ttttgttatg agggtcgata taacgcaggt aataccatga gcttcctgcc cattgtggca
    11821 ttgtattcgt ttcgcgacgg ccttttttac ctgttacagg atcaacgaca ttgacccact
    11881 cctcgatatt tgctaatgga gattctccag taccgcttgg cttgatgttt tttgtcttag
    11941 gcaaccgtaa tggtagctct tcttctggga cagctgtgac tgttccgtcc tcccaatgga
    12001 tgattgggat tggttcaccc cagtaacgtt gacgggagaa tagccagtca cgtaagcgat
    12061 agctgacttc ttttttccct acgccatttt cttctagcca ttcattcatt ttatcgattg
    12121 cttcttgttt gttcatgccg tctagaaatt cagagttgat gtgcgtacca tcttctgtgt
    12181 aagcagcttt ttcaacatct ccaccttcga taactggaag aatttctagt ccgaaagttt
    12241 gagcgaattc aaagtcacgt tcatcatgtg ctggaacagc catgatggct cctgtgccgt
    12301 atgaagataa tacataatcg gcgatccaga ttgggatttc cttgccatta actggattga
    12361 ttgcataagc accagtaaat actcctgttt tttcttttgc aagatccgtt ctctttagat
    12421 ctgatttttt agctgtctct tcgatataag cttctacagc agctttttgt tcaggggtcg
    12481 taatttctct aacaagatct agttcaggtg caagaaccga gtaagttgca ccgaatagag
    12541 tatctggtcg tgtcgtaaat acggtatagg atttatccgt accagccact ttaaattcaa
    12601 tatttgctcc tactgaacga ccgatccaat ttctttgcat ttctttaatg ctttctggcc
    12661 aatcaactaa ttctagatca tccaataagc gatcagcata agcagtgatt ttcagcatcc
    12721 attggcgcat tggttttctg actacatcat agccaccacg ttcacttttc ccatcgatca
    12781 cttcttcatt tgaaataact gttcctaatt caggtaccca gtttaccgct acttctgctt
    12841 cataagctag acctttttca taaagcttcg taaagatcca ttgtgtccat ttataatact
    12901 ccggatcagt tgtattcact tcgcgattcc agtcataact aaatcctaaa gaattgattt
    12961 ggcgtcggaa tgtttcgata tttttttggg taaactcagc tggatcgttt cctgtatcta
    13021 gcgcatattg ttcagcaggc agaccgaatg catcccaccc cattggatgc agcacgttgt
    13081 atccttgact tcttttcaca cgtgaaagga tatccgttgc cgtataacct tcaggatgcc
    13141 ctacatgtag cccttgaccg gatggatagg gaaacatatc cagtgcgtaa aattttggct
    13201 tttcaggatc atcatgggtg ttgaaagtat tcgtttttgc ccagtatttt tgccattttt
    13261 tctcaatttc tttatgattg taactcattt gctgtcctcc tgttctattg aaaacacttg
    13321 tttctattat aaatataacg aaaaaaccgg cagaatctgt ttatttttat ttccttttgc
    13381 tttgttttct caaacttcac aaaagaaata aaaaaatcct ataaaaagat tgctcttttt
    13441 ataggacgta atctacgcgg taccacctaa ttttacctta gtgaaaaggc ctcgaacgtt
    13501 gtaacggccg tcaccgccgc ttgcttactc gggtattcag caagggcaac tcaaaggcga
    13561 gttcaaaagc atccgtgcac attctcacca gccatgcgct ctctgaaact tcagtctttt
    13621 tactacttcc ttttcaagtg ttgtcatatt taagtagcct tatagtaaca aatatttttg
    13681 tggaattcaa tcgttatttc ttcgaacagc tattttattt agctacaatc aacgtttgac
    13741 ctggataaat aaagttgttc ttaatattgt tccattcgat tagttgagcc attgtaattc
    13801 catgactgtc agcaacactc caaacagatt ctccagattt cacagtatag gttttcgaac
    13861 cagtaactga ttgttggttt gttgattgag atgaaccttt cttcacaatc acttgctgtc
    13921 ctggatagat gaaattattt ttgatattat tccattcgat taactgatcc atcgtgatat
    13981 gatgtttgtt cgctacgctc caaactgact cgcccgcttt cacggtatac atcgtattac
    14041 cagtggatga tgtacttgtc gtagcagacg tattcggtgt atttggtttc gctgcagaag
    14101 tagttgaacc tgtatttgta tttgccgaag ttccaccttt aacgatcaat tgctgtcctg
    14161 gataaatgaa gttgtttttg atattattcc attcgattaa ctgatccatc gtgatgccgt
    14221 ttttatttgc cacgctccaa acagattcac ctgctttcac ggtataagtg ccttgagacg
    14281 aactgctgcc agtattaact gaactgctgt tatttgtatt ggttgacgaa cctgtattac
    14341 ctgacgtgct gtttgttgat ccactgtttg aaacagtcag ttgttgtcct gggtagataa
    14401 agttattttg gatattgttc cattggatta gctgagccat cgtaattccg tatttatttg
    14461 agacgctcca aacagagtct ccggctttaa ctgtatattt cgttcctgaa gctgtattag
    14521 aagaacttga gctggacgaa ctagagccac ttgtattccc agtatttgag ccagtatttg
    14581 ctccagcatt agaagaaccg ccatttgata cgattagctg ttgtcctgga taaacgaaat
    14641 tgttcttgat attgttccat tcaatcaatt gagccatcgt aattccgtga tcatttgaga
    14701 tcttccaaac agaatcacca gattgaacag tgtatttcgc accagttgtt tgtcctccgt
    14761 ttgatgtacc agcatttcct gaagagttgg tattgttacc ggaattattc gtcgaagagc
    14821 cattttcctg gctacctttg attgtcagtt tttgtcctgg ataaatgaaa ttgttttgga
    14881 tgttgttcca ttggatcaat tgatccattg tgataccgaa tttatttgag attccccaaa
    14941 cagaatcacc agattggaca gtataagtgg tcgaagttgc tgaacctgag tttgtattac
    15001 cagaattgct tgaaccgcca gtactacctg cacttccagt attattgcca cctgtagtcg
    15061 ctccaccgcc tgcgtttccg ctagctggtg tatcatattg ggtcaatccg taagttgcga
    15121 tgatattatt taatttgcct gcgtaaccag gatcagtcgc ataacgccct gtcaaacaag
    15181 ctgtcgcatc tgtataggat ttcgtgttgc tcttccaagt accggcatag taataaccgt
    15241 tgccaaatga agtatttcta agtacatagg cattgtcatt gaaagattca gcaaaagaag
    15301 gatattgtcg gaaaggctcc tttttattta cccattgatt atttaaaaat tcaagcgtat
    15361 ccatgtagac ggtttgtcct tgatagctgc cttttatccc aaaaagatta taataaggtg
    15421 cttgagataa cgcactactg ccccaaccgc tttctacaac tgcttgtgcg atcatcactg
    15481 atgcatatag atcgttggct tgagcgattg gttgagcata agtagcaatt tctgtgataa
    15541 atgccgctgt attgattctt gcgccgaact gaacaggtgt ttcatccgct tgaacaggaa
    15601 ccggctgtac taatggggca gcgacagaac agcctaccat cgcagttccc acgattgctg
    15661 cccctcgttt tgcttttcga aaacgtttgg tctgttctaa acgtctccgt tcttttcttg
    15721 caatgttttc catcttctat cctccaactt caatttatct attattaata attataactt
    15781 ctcttaatat ttattaaaag ttatttaaga gctttgttgt tttttgtaat catattgtaa
    15841 tttaccgtaa tgaacaactt ctgttcattt tcaagttctt cctacgtcta cgttcttgtt
    15901 taaaaggatt tcgaaaaaca ctgattttat ttccctaaca tttcatttgc attaccaaaa
    15961 aatgtctttc cgattacagt tatttttttt ataagaatat tggttattca aagaggaatc
    16021 atacattggt tttatctctt taccgttttt ttcgctggac caaaaatgct ataataagtc
    16081 attgcttcaa aggaggaatt gtatgaaata taaaacatca tttcaggtgg ctggtagctt
    16141 ctttcttgtc ctgtttttca ttttgggctg gatcgtaaag ttccatctgt cgtggctttc
    16201 tggtttcgat cagtggttta caggcattgt cagagcttct tatcctgatc tgaacagcta
    16261 ccaactcttt atgactaaat tcggtaatcc tcttaccgtc atcattttat ttgcttgtgc
    16321 ggctttatgg atgtggtata agaaaaaaag ggcagaaaca ttttggttcg tttctggatt
    16381 tattgtaatt gctggaatca tcaacccggt gatcaaacta tttttcatgc gagagcgtcc
    16441 ttcattggcg cacttagtag ttgaaacaag ttacagtttc cctagtggac atgcagcagg
    16501 cagtatgatt ctttacggtt ctttactatt tctgcttcca gcttttgttc aaaaaagaac
    16561 cttacgcaca ttgcttcaat tgctgatgat gtttattata gtaagtgtcg ggataagccg
    16621 catttatctg ggcgttcatt ttcctactga tatcatcggc ggctacttac tcagtctttc
    16681 ttggctgtgc ctgacttatc cagtgtatag agaaaaaaga aaacaaatag ataactctta
    16741 cagaaagaag gacttttaat gacttttcct tattcagaag gaaataaaag atatcattct
    16801 tggaattacg ctttacgaaa tgagtttggc gggaaaattt tcaaggtgcc gatcgatgga
    16861 ggatttgact gtccgaatcg agatgggact gttgcccatg gcggttgtac tttttgcagt
    16921 gtatctggtt cgggagacat gatcgttgca cctgaagacc ctcttccaat ccaattccag
    16981 aaagaaatcg acatgatgca tcaaaaatgg cctcatgtcc aacaatacat cgtttatttc
    17041 caaaatttta caaacaccca tgcgccagtt gaaataatca aagaacgatt tgagcaagta
    17101 gtcaatttac caggtgtcgt aggattatca gtcggtacac gaccggattg tctgccagat
    17161 gaagtagttg attaccttgc tgaactgaac gagcggatgt atttatgggt agaacttgga
    17221 ttgcaaacaa cttttgaaga aacttcaaaa ctgatcaacc gagcacatga ttatcaaact
    17281 tatttagatg gcgtggctaa attacgcaaa cataatatcc gtgtttgtac gcatttgatc
    17341 aacggattac caggcgaaag tttagaaatg atgaaagaaa atgtcagacg caccatttta
    17401 gattcagata tccaaggaat caagctccat ttgatgcatc tgatgcgcaa aacgagaatg
    17461 ttgcgggact atcatgaagg tcgccttcaa ttaatgagcc gtcctgacta tgtcaatgtc
    17521 atttgcgacc agctggaaat gataccaaaa gagatcatca tccatcgttt gacaggggat
    17581 gcgccgtggg attccttgat tggaccaatg tggagcttga aaaaatggga agtcttgaat
    17641 gcaattgatg aagaacttct tcgcagagat agcttccaag gaaaatatga tgtacggaaa
    17701 aaggtgagcg tctgatgttg caaactgctt tgcatttcag ccatacatta ctgcaagaaa
    17761 tcttgcaacc aggggatcac gtcgtagatg caacgatggg caatggccac gatacggttt
    17821 ttctagcaga acacatcgga aaaactggtc atgtctatag ttttgatatc cagcaacaag
    17881 caatcgatgc tactcgtgaa aggctagaac agcgtcagtt agaagaacgt gtctcattgt
    17941 ttctacaagg ccacgaaacg ttaggagaag tgatagctga acaacaaaat ctcaaagcag
    18001 ggattttcaa tctagggtat ctcccaaaaa gtgataaagc aatcatcact atgccagaga
    18061 ctacacgcac agctatggaa gaaatattga aacgacttgt gcctcgcgga cgattgatcc
    18121 ttgtcgtcta ctatggtcat gaaggtggag aaaaagaact ggatatggtt gattcttttt
    18181 gttccaaact gccacaggaa acatataatg tgctgaatta tcggtttatc aaccaaaaga
    18241 accagccgcc tattttatat tgtattgaga aaaaaagata aataaaaaac gcaaaacata
    18301 aatgatatgt tttgcgtttt tttctctaat actcaggtta gagcgttgat ttcataacct
    18361 ctttattcac ggtgctcaaa agcttatatc tgcggtaata agtccaacca aatcaaaaac
    18421 atgagaagct gtttttgatt ggctgttctt attacttgat gcaggacagc tttttcacaa
    18481 cctctttatt caaatacaaa ttgaaattga acacattttt ttgacatata acagatatat
    18541 aaaagaatgg caaaaacatt gatatataag catttttaaa agattttata tttttgtatg
    18601 tcattcatat atcaatagct aacttaatat cagtcaaatt atgccccttt tttgcccctc
    18661 aaatatcgct ttacaacaag aacgtaagtt cgtataatcg ttttgaggtg atttttatga
    18721 tggaagaatt tattagaaaa aatatcagtg atgaatatgc agacttttat gatcaaagca
    18781 gtaaaaaaga taaattccag atggatgttt caatcttagc tatattagct ttttccgaaa
    18841 ataaacaacc tgtaactgca aaaaaagaaa cagtattctc tgaaggcaaa ataaaaactc
    18901 gatatatatt agaggtagaa actaatttta aaaacagatc ggagtaatgg ttatgctttt
    18961 taatgag
//
LOCUS       DAZABD010000049        18415 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000049 DAZABD010000000
VERSION     DAZABD010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.049"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..459)
                     /locus_tag="ACHALG_001915"
     CDS             complement(<1..459)
                     /locus_tag="ACHALG_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFN2587296.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEG"
     gene            complement(631..990)
                     /gene="comGG"
                     /locus_tag="ACHALG_001916"
     CDS             complement(631..990)
                     /gene="comGG"
                     /locus_tag="ACHALG_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGG"
                     /protein_id="HFN2587297.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            complement(962..1387)
                     /gene="comGF"
                     /locus_tag="ACHALG_001917"
     CDS             complement(962..1387)
                     /gene="comGF"
                     /locus_tag="ACHALG_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="HFN2587298.1"
                     /translation="MKKQVIPAFTLIECLIGLMILSSLLLTFNLLIQQTIQVSEFLQR
                     KDQKEWLIFLAQLEEELKSSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQ
                     PMLTSITELQLLEKEHTISFYVHFENGEDGYAIWTKNDQ"
     gene            complement(1350..1697)
                     /locus_tag="ACHALG_001918"
     CDS             complement(1350..1697)
                     /locus_tag="ACHALG_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587299.1"
                     /translation="MVAIQKELNKTGFVLLESLTALMIASVCVYLVSFGQLFLLQAEK
                     KSGEELLFIRMIYEDIKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAI
                     SIEKTSNSSIYAD"
     gene            complement(1669..2139)
                     /gene="comGD"
                     /locus_tag="ACHALG_001919"
     CDS             complement(1669..2139)
                     /gene="comGD"
                     /locus_tag="ACHALG_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HFN2587300.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            complement(2114..2392)
                     /gene="comGC"
                     /locus_tag="ACHALG_001920"
     CDS             complement(2114..2392)
                     /gene="comGC"
                     /locus_tag="ACHALG_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus major pilin ComGC"
                     /protein_id="HFN2587301.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            complement(2389..3438)
                     /gene="comGB"
                     /locus_tag="ACHALG_001921"
     CDS             complement(2389..3438)
                     /gene="comGB"
                     /locus_tag="ACHALG_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HFN2587302.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            complement(3395..4324)
                     /gene="comGA"
                     /locus_tag="ACHALG_001922"
     CDS             complement(3395..4324)
                     /gene="comGA"
                     /locus_tag="ACHALG_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HFN2587303.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            4456..6210
                     /gene="ade"
                     /locus_tag="ACHALG_001923"
     CDS             4456..6210
                     /gene="ade"
                     /locus_tag="ACHALG_001923"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFN2587304.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            complement(6308..6970)
                     /gene="trmB"
                     /locus_tag="ACHALG_001924"
     CDS             complement(6308..6970)
                     /gene="trmB"
                     /locus_tag="ACHALG_001924"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFN2587305.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(7040..7822)
                     /locus_tag="ACHALG_001925"
     CDS             complement(7040..7822)
                     /locus_tag="ACHALG_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HFN2587306.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            complement(8026..9237)
                     /locus_tag="ACHALG_001926"
     CDS             complement(8026..9237)
                     /locus_tag="ACHALG_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN2587307.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            complement(9230..9967)
                     /locus_tag="ACHALG_001927"
     CDS             complement(9230..9967)
                     /locus_tag="ACHALG_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN2587308.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            10105..10539
                     /locus_tag="ACHALG_001928"
     CDS             10105..10539
                     /locus_tag="ACHALG_001928"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFN2587309.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            10532..10876
                     /locus_tag="ACHALG_001929"
     CDS             10532..10876
                     /locus_tag="ACHALG_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFN2587310.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            11056..12066
                     /locus_tag="ACHALG_001930"
     CDS             11056..12066
                     /locus_tag="ACHALG_001930"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFN2587311.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(12135..13079)
                     /locus_tag="ACHALG_001931"
     CDS             complement(12135..13079)
                     /locus_tag="ACHALG_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFN2587312.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(13063..15783)
                     /locus_tag="ACHALG_001932"
     CDS             complement(13063..15783)
                     /locus_tag="ACHALG_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFN2587313.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            complement(15780..17000)
                     /locus_tag="ACHALG_001933"
     CDS             complement(15780..17000)
                     /locus_tag="ACHALG_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFN2587314.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            complement(17281..18126)
                     /locus_tag="ACHALG_001934"
     CDS             complement(17281..18126)
                     /locus_tag="ACHALG_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFN2587315.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
BASE COUNT         5668 a         3663 c         3039 g         6045 t
ORIGIN      
        1 tccttctgtt tggtaccccg cattcgatag gtttaaaaga actgttaata acaagttgcc
       61 cattcctgcc gcaatatcca gaatcttaac cggtgctttt ttatttgtgt atagttgttc
      121 aataagaaaa acaaataaaa aaccgatact atcaggagtg agttgatggt tcgcctgcaa
      181 atgttctgtc tggctgccct tcaataaaag taattgagac agtctgcgcc attcttctgg
      241 ctcgaaagaa agtttcttca actcttcata aagagctgtg atccgttgcg ttgtctcttt
      301 tgtcggcact ccatccacca cacgtacttg ataatcatct attaaatttt ctgcattttc
      361 gacataggct tctaaaaagg aagttcccaa tgcattctgt aaaagctgaa tagcttccag
      421 gttttgatta aacccttgtt ccattttttc tggtaacatg gtgcacctca tttctagata
      481 tattataaca attttttcac ctgtttaatt ctatcgaaca attgacaaaa tttcaatcgt
      541 tttcactgtt ttttatttgt gaaacggcaa acattttttt attaaaaaca gtgttataac
      601 agaaaagagg gcgttttcac gaacattcca tcattctgta ctagtatctg tatcattttt
      661 tacttcttct gtgaaccgaa agataaacgg tgagacttcc acctctaaga atagggactg
      721 ttgtacccac tgatatgtta cagatccagt tgtgtagtaa aaaactcctt ccgaactttc
      781 ggcaagcgta gactgttccg ataaagccat ctgcttcatt atttttgcta tataataatt
      841 tttgattcgt atattaagat ccattgacaa acggtagctt tctaatgctg ccatcaataa
      901 aaacgtacat aaaagaaata aaagcaagac agaaaataaa atagagccct tataacattt
      961 tctactgatc gttttttgtc catatggcat atccatcttc tccattttca aagtgtacat
     1021 aaaaagaaat tgtgtgttct ttttccaata attgaagctc agtgatactt gttaacatag
     1081 gttgatgtcc gccggaagtt cttcttttac gtatcattgc ctgatatctt tcgatgatat
     1141 actgtttatc ctcgatcgtg tagtgcagtt gctgattttt cacagagaca ttgtgactgc
     1201 tcttgagttc ttcttctaat tgtgctaaaa atattagcca ctctttttga tctttacgct
     1261 gcaaaaattc acttacttgg atggtttgct gaatcaaaag attgaatgtg agcaatagac
     1321 tgctcaatat catcagtcca attagacatt caatcagcgt aaatgctgga attacttgtt
     1381 ttttcaatac tgatcgcttc tttctccata ttgacaaaca ctttttcgat tactccatta
     1441 ttatcgacaa tttggatatc gtatgggatc atttcttccc ttcgctctgg tcttttgtga
     1501 tacatccgct gcgtcttgat atcttcataa atcattcgaa taaatagcag ttcctcccca
     1561 cttttctttt ctgcctgaag caaaaacagc tggccaaagc tcactaagta gacacagaca
     1621 gaagcgatca taagcgctgt aagggactcc aacaaaacaa atcctgtttt attcaattct
     1681 ttttgtatag cgaccactcc ctatctggaa ttgatacgta atcgtccgct ctttttcttc
     1741 ccaataaaat tgataagctt ttagtgaact ttcattcccg gttctagcag caaaagtaat
     1801 agaagcatgg cgtttcaagg taatctcatc aggaatatct aatacttccc aatttatttt
     1861 atctggattc ggaacatgaa aaatgatttg attctcttca ctattccaat aaaagcctgt
     1921 ttggatctga ttcacaatag cgattttttg cgtagaatag attcgttttt caaattggcc
     1981 gaaaaattga tagactgcca attcattctt ccatgcagaa aatgcgagaa ctggaaaaga
     2041 aaggatcgtt gtaagtaata aaatcagtaa gagtgattca aacaacgtat atcctgatgc
     2101 taaaacctta ccttcaaggc cgctgataga tttctgcatg ttctcgtttg atatagcctt
     2161 cattcaacaa ttcctccaca gtaggaactc ttccattatt ttgaagcgca tagagctctt
     2221 tttgtgtgtc taccaactgg atgatcgctt cacgactttc ttggtctaca tgattcctat
     2281 aacgcgacaa attagggacg aacaacaaca ctaatacact gatgatcagt aagacgatta
     2341 gcatttcgat caatgtaaaa ccgctataat aagatatttt tctctttttc atggcaaaac
     2401 ctcctctatg cttccgtaaa caggtaataa aacagcggca taaactaata aaatcaaagc
     2461 cgctacgccc aaaaaaatga ttggctgaat aaaatgcatc cagtgatgaa gacgtttgaa
     2521 aagctgtttt cttgtccatt cgctgtaaaa aatcagttct ttacctaaac ttccctttgc
     2581 ctctccctgt aaaacaattt tactgaattc ttctgataga aaacgataat tctggaactg
     2641 ctcagccaat gacatacctg cctccaatcc ttgaatcaac ttagaggcaa gatgctgaat
     2701 cagactttct tgtttcgttt cctttaagca gactatgatt tgccttaatt cgaaaccttc
     2761 atgaaaaagc tttccaatct ctagtgacaa ataagccgat tgatacaagg aatatatcgg
     2821 tccaatcaat aattgtctgc ttatccattc actctgatca acaggagaca acttcatcag
     2881 acgatacctt actagtaaag ccaatagagt aaccgctagg atgaatagta gaacatacca
     2941 atgaaagact tgtatcagct gaatacccca atgctcttgc atgaccattc ctgaaccaat
     3001 aagctgaggc aaaacgaaca accgcatact aaacaagata cctagcaaaa aggtgagcaa
     3061 tagcatcgga tatgcaagca gctttttcaa atcattgcga aatccttcaa caagccgaaa
     3121 ctgtttggca atttccaaca aagttgtcgg cagattccca tgacgttcag ctaactcgat
     3181 ctgtaccagt tgctctgctc catatcctat ttgcgaaagt acctcactaa acgcttgccc
     3241 ttcctttagc tgatgttgga tatttgccaa aataacaggt ggaaacgaac ggatcgtgag
     3301 taataaataa agggattctt gcagactaaa accagataaa agcagttctc ccatggtttg
     3361 tgcaaactgg ttttgctggc ttcttgttaa tcctttagaa tagttctgac tgccaaatat
     3421 tttcctgagc ttcttcataa ctttcctccc atgatttttt tactgattct tcctccataa
     3481 attgataact ccataaaaca gaaggatggt gattggcatc ttccaaaagt tcttgataaa
     3541 tgacaccagc taaacattca gatagctctt cttttttctt tgtcagttca tggattctga
     3601 ccaatgtacc ttcaagattg cgtgcatgta cggttgcaaa aacccggtgc cctgttagtg
     3661 ctgctcttac cgcagcatgt gcagttgatt catctcttat ctcgccaatg attagcaagt
     3721 ctggacgatg ccgtaatgcg agtctcaaaa gtgtatcata agtctgtgct attttcggat
     3781 tgatctgtag ctgcagaaaa aacggttctt ctatctctac aggatcttcg atagtaatca
     3841 cttgcaattt ttcttccaaa gccaaccgat acattagaga agtcttgcct gatccgactg
     3901 gtccgctgaa aaggtataat ccacgtctga cggttttctg taaaatagtt gcaaaatctc
     3961 gagaactaaa gcaacgatgg ggtgtttcaa aagtaccgta taatagccga atcactaaac
     4021 tctctcgttg aaggtaatct cctacagaag acaaccgcag acgaacttcc tgtttttcta
     4081 aagaataagt aaatgcacct aactgagctt ttcttttttc tccgatatcc atctgtccca
     4141 aatacttaaa tcttgagatc agttgctgtc catctgccga agagactttc ttccagaatg
     4201 ttcgtttttt tccatagcga aaatagatgt aaaatacttc tgaagctggc aatatatata
     4261 cgtcttgggc atgtttggca aaactttcac ttatcatttg atccgataat cgttttattt
     4321 ccattattct cccctccaca aaaaacatac gtctttttga tctttttgtt tcaaataaaa
     4381 tgaatttaca aaaaaatatt ggcaaaaacc gaatttatgt tatacttata aaaatttatc
     4441 ttgggaggca ctccattgga aacaacatta aggaaatata tcgaagtagc aagtaaaaaa
     4501 aagcctgctg acctagtgat caagcatgct aagatcgtca acgtctttac gaaagaaatc
     4561 atggaaaaag atgtagcaat ctgcgaagga atgatcgtag gaatcggtga atacgaagga
     4621 aaaagcgtac ataatgctaa tgggcaatat ctagtgccag gattcatcga tggccatgtg
     4681 catatcgaaa gctcactttt atcaccaagc gaattttcaa aagtatcctt gcttcatgga
     4741 gttactactg tagtgacaga tccccatgaa ataggaaaca ttgctgggag tacaggtctt
     4801 gagtttatga tagaagatgc aagacaaaca ctcatgaata tcttttttat gctaccttct
     4861 tgtgtacctg taaccccttt tgagaccaat ggtgcaaatt taaatgcaaa aaaattagcc
     4921 ccttttttac aaaaacccga agttttaggc ttagctgaag tcatgaacta tcaagcagta
     4981 gcaaataatg aaacagacat cctagaaaag atccaattga tgcaccagca taagaaaaaa
     5041 attgacgggc atgcagctgg tatcggcatg gaagagttaa atgtttatcc tgcagcaggt
     5101 attcggacgg atcatgaagc tacaactgca aaagaagcta aagaacgttt ggatcttggt
     5161 atgtatttaa tggttcgaga agggaccgtt gcaaaagatc tggcatcgct actaccggcg
     5221 atcacacctg aaaattcaag aagatgcttc tttgtcaccg atgataaact gatcaatgat
     5281 ttagtaaaag aaggatcaat cgatcatatc gtccgtcaag ctattgcact tggaatcgat
     5341 ccattacaag ccattcagat ggcaacattg aatgcagcag aatgttttga tttgaaaaca
     5401 ctaggtgcga ttgcccctgg gtatcaagct gatttcttct taacagacga tcttaccaag
     5461 ctgccaattc atgaagtttt ctataaggga acattagttg ttcaatctgg gcaactccaa
     5521 gaccatttat ttgctgatca ttcaaattca tatacgtgtt gtctgccaaa actaaacgct
     5581 caaccattaa atccacattc gttagagctg cttctttctt cttcacaagc ccatgtgatc
     5641 gaaattatcc cgaatagtct agtgactaaa gatttggtag aaaaagtgga ccgacaaggc
     5701 gaacgattca ttccttcaat cgaaaaagac caattaaaga ttgcggtgat cgagcgtcac
     5761 catcaaacgg gaaacattgg attaggcata gtgaaaggat ttcagctgaa acaaggagcg
     5821 atagcgacaa cgattgccca tgactcgcat aatctagttg tcgttgggac aaacgacgag
     5881 gatatgctgt atgctgctaa tcttttgatt caaaaaggtg gcgggatgac cgttgtcaac
     5941 aaacagcagg aaatagcttg tgtttctctt cctataggcg gaatcatgac acaggaacct
     6001 ttttcgatcg tctatgaaca gttgaatgaa ttaataacta aagcacatca actaggagca
     6061 gctaaaacct ttgatccttt tttgacactt tctttcttaa cattatctgt catacccgaa
     6121 ttgaagatca ccgacaaagg attattttca ttttctcgct tccaattgat tccaccttgt
     6181 ggaaaaaccg atgaaataaa tacattttaa atcaaataaa tcccagaaaa tacattacat
     6241 ctgtaatgtt cattttctgg gatttattta tggatattaa tgtttttatg aaagcagaaa
     6301 gttagtttca cagacttttc tttttggtct tatcttgaaa ttgagcttct actcgataaa
     6361 tacgttgacc ttttgaagaa aatttttctt catactccgt cataatattg ccttcaaatt
     6421 gactttgatg taaatctaac catacctgtt tgaggatcat gccatattga gaaaaacttg
     6481 ccagagaata ctcaaacaat ccttgattat ctgttttgaa atggatttct ccatttggac
     6541 gaagaatctg ttcatctaca gcaagaaaag tcttatatgt caatcgccgt ttttcatgac
     6601 gtttttttgg ccatggatcg gaaaaattca gatagatttg atctacttcg ctgtcagcga
     6661 aatactctgt caacgctgat ccatccacat gtagaagttt caggttagga agcggttgtt
     6721 cgatcaactt gtctagtgca atcgaaacaa cactgacctg catttctact ccaatataat
     6781 tgatttcagg atgggctttt gccattccag tgatgaactg gcctttcccc ataccaatct
     6841 caatgtggat tggatgatcg ttttcaaaca attcattcca ttttcccttc cacaatgttg
     6901 gatcactaat tacaaaattc gggtgagccg ctaacatttc cgctgctccc ggacgatttc
     6961 ggacacgcat tatttatctc cattcctttc ctctaattaa aaaggtctgg ccaaatctcc
     7021 gcagagtttg ggccaaacct cagccactga aaattcgttt taattgtaaa atttcttcgt
     7081 tcatccgtcg atgatcttca cgtttgcaat atctggtgat ttcttggagc aaattcatgg
     7141 ccgcatacca atgaaatttt tcaagcattt cgtcagtcgg gcgtatgcca taactgatca
     7201 gccactgatt ccagctagac agtggcacat aatttcctaa aatcgttgca atatccaccg
     7261 cagggtctgc aaacatgatt gaatcccaat cgactagata taaataattt ttacatacta
     7321 accaattacg atggtttaca tctccgtgta ctactgataa gttttcctta tctattgacg
     7381 ggatctgctc acgaagaaaa cgatggacaa tttgcagaaa ttgatttttc tggagttctt
     7441 ccggtaattc tttttcatat ttttgcagca tcttctccgg cgtcatgaca cgaccaccga
     7501 tacgggtcag catggtttta agagaatttg aatgatgtaa atggtacaaa acatcaatca
     7561 cgtcgtttct ttgaccaatc tcattaggag tcagcaagcg accttccagc cactcttgtg
     7621 ctgtcaaagt atcgccattc cccgttcgtt ttgtccaaac aagttttggt gtaattcctt
     7681 cccttgaaag agcagccaac atcggagtcg tgtttcgttt aataaagact ttgtcctcat
     7741 cacgcatgcc tatgtatgtt tttcctgtgt cgcctttgat gggacgtaac cgccactctt
     7801 ggtctaactg aaactccatc atgcattctc ccgtcctctt actctttgtt ttgcagtact
     7861 tattattcta gtcgcctgaa cactacacgt caagcacata tacgttcttt ttcatttaat
     7921 tgccattcaa aaaaccagct tttctcaaaa aaaatagaac gaacagcatt cttactgtca
     7981 acgctctatt tttcattctc atttttaccc tacacagctt tgttttcagt ctgacatctt
     8041 cttcaaacga gttggtacat atagtttaac aaagagcata gtaacaatca aataagccgc
     8101 tagcggaacc catcgctgct gcccatctaa tacgactgca gcaatcaaac caaaaataac
     8161 cgacgcgata attaataccc atgtgagtag atactgaatc gcctgcttct tctgattctt
     8221 atggattgga taaagctgaa cgagaaccat atagcggaac tggttcgcta aaggtaacag
     8281 ttggaaaccg atcaaatata aaaataagaa accgataatc agactaaaat aaaagtcgac
     8341 tactccggca atcaataacc cacctataat tactaatcgg aaataaagac cactaaaatc
     8401 agaacctctt gccaatcttc ttgcaaacaa gtaaagataa gtgttttttg attctctttt
     8461 aatatgagtc aatataggat ccaaatagcg tcttcttttg atctttgccg taatttcagg
     8521 aacatctgtg aataaattga taaattgata gatctgatgc aacctatgtt cttctacatg
     8581 caccattttc tcccaatcca atcctttttc ttgttgcttc cacaaaaacc agtaatatag
     8641 acttgcctgt actaatgcac ctgctattcc tgcccatatt agccaataaa atgatagaaa
     8701 caaaaagatc agagaagaaa aaaaccagcc taatttccaa taacgaataa cttgttgatc
     8761 tagtcgaaac aattctgctc tcttgatttg cagatgactg aatttcaaac tccataatat
     8821 gatcagatag acgaaaaaca tcgaaaagga ttgctccgta ctaaccacca ctaatggcat
     8881 ggccaatccg catgccaata tcaaaaagat caacggaaaa atgcaagaat agcgaaaagc
     8941 tcgttccaaa taggtttgca tttctttttc tttaggaagt aaaaaaacaa tatctgctgc
     9001 ctctgcaaaa gtagccatct tacccacgaa taaacagagc atccaaaata cagagattat
     9061 gagccctcca attgcaaacg gtggctgcag ggttttcaac caattagaat aatataatcc
     9121 cacaccgcca atcaagaaaa gacaaactaa aacaaaatga tcgttcaata cataacgcat
     9181 atatttcatc attttctttt gatgtcttgc taaacgtttt tgaaaaaaac tactcatgtg
     9241 cctgctcctt tgttaaagca atatagattt cgtccaaaga agaatccggc agctgaaatt
     9301 cttttctcaa catctcaaga gaaccgtctg ctcgtatctc tccgttatgt aagaccacaa
     9361 aacgatcaca atatttttct gcagtagcta aaatatgcgt agacatcaaa ataccggctc
     9421 cttctttttt catttctacc atcaattcaa gcaatgcatt gatagctagt ggatccaagc
     9481 ctaaaaatgg ttcgtcgatg atatataaag aaggctcgat caaaaaggcg cataagatca
     9541 tcactttctg tttcattccc tttgaaaaat tagctgggaa ccagtcaaga cgattagata
     9601 accgaaaagt ctccaacaac ttttctgctc ttttcattgc tatatccata ggtatatcgt
     9661 aggccatcgc agtgacttcg atatgttctc tcaatgtcaa ttcttcatat aaagaaggag
     9721 tctctggaat atacccaatt tgtttgcgat attcttccgt atttttcttt aaggatagat
     9781 gattgatttc tatcgttcct tttgaagctt ccagcagacc gataatgtgc ttgatggtcg
     9841 tacttttccc tgctccgttc agtccaatca accctactat ctctccaggc tgtacatcga
     9901 aactgatatc ttttaacaca gggagatgtc cgtacccccc tgtgacatga tcgataatta
     9961 aactcattct ttttcctccg ccatttattt ttatttatta taccacgaag aaaatgacgg
    10021 tattttgttt ttttccttac aatagcttta acttatggta aagttaagct gaagtattta
    10081 ttctttatag aaaggatgtt ttatatggaa aactgtattt tttgcaaaat catcaatcaa
    10141 gaaatcccaa gctataaaat atatgaagac gacaaggtct atgctttttt agacatctct
    10201 caagcaacga aaggacacac attagttgtg cctaaacgtc atgtagcaga tatttttgaa
    10261 tatgatcccg aacttgcagg agaggttttt tcgcgtgtgc ctaagattgc tcaagcattg
    10321 gaaaaagctt ttccagaaat gaaaggtctt aatatcttga ataacaaccg tgaactagcc
    10381 tatcaatctg tttttcattc acatatccat ttagtccctc gctacagcaa agaggatgat
    10441 ttttctattc actttgtcaa tcatcaagat agttatggat cggaagaact aaaagctatt
    10501 caagaaacaa tcgtaaagca ggtgtcctgc gatgattaaa aactttttca aaggactgtt
    10561 atttggagct gctgctggca ctgtcggagg tctgttattg gcgcccagga gcgggaatga
    10621 aacaagaaag aaactgatcg aagaattgga agaagcaaca actctgacaa atgatttaaa
    10681 taacagcctg aatcatttta aaagcgcatt gatcgaaaca aaagaaacag ctgaatctgt
    10741 gattcctgct ttccaagaag caatcaaaaa agatatcgaa gattttaaat tccaagccga
    10801 accacgtatc acacaaatca atgaacaagt agaaaaacta aaagctgatc tgccagagta
    10861 tcctgaacaa atataataat gattattttc tcaacagatt taactatata agtgagaaaa
    10921 gtttgcgtat ttcctaggaa tgtgatgaaa ttttgaattt tgtttgaaac taccacgaca
    10981 acgtttttaa aggaaatggg atatggtata gttaatatat aaaaataata actaattaca
    11041 ggagtgcaaa ttttaatgaa aaaaaaatca attattttgg cagctactag tgcgttggct
    11101 gttttaactc tagcagcttg ttcaggcgat actaataaag atatcgcaac gatgaaaggc
    11161 ggaacgatca ctgtatctga tttctatgat gaagcaaaac ttgaatcatc gaaccaatcc
    11221 ttagtacaac ggatgatcat ttataaagta ttcaataaca aatatggcga taaagtaacc
    11281 gataagcaag tggatgcaga atacgacaaa caagcgaaat cattaggtga tacttttgaa
    11341 tcacaattag aagctgctgg atatacgaaa gatacttata aagaatatat tcgtaacaat
    11401 ttagcttttg aagctggctt gaaagctcac gtagatatta cagatgacga tttgaaaact
    11461 gcttggaaat ctttccatcc agaagtagaa gcacaaatca tcaaattatc aagtgaagat
    11521 gaagcaaaag atgtgaaaaa atcagcggat gatggagatg atttctctaa attagccaaa
    11581 gacaaatcaa ctgatacaga gacaaaagaa gatggcggta aagttaaatt cgactcaacg
    11641 acaacaacta ttcctgcaga agtaaaagaa gcagctttca aattgaaaga cggtgagatc
    11701 tccgatgtta ttactacaac aaatcctact tcttatgcga cagaatacta cgttgtcaaa
    11761 atggtgaaaa accaaaataa aggcaatgac atggataaat acaaagatca actaaaagat
    11821 atcgctactg aaactaaact aagtgacaat gcattcacaa ctaaagtaat cggtgaagaa
    11881 ttaaaagatg caaatgtaaa aatcaaagat gatgcctttg aaaatgtgtt gagtgcattc
    11941 acaacaactt catcaagtac gaaagactca agtgaaacta cagcttcaac aaagagttct
    12001 gacacaaaat caactgattc aacaaaagaa tcttcaactg aagaaacaac tgattcatca
    12061 aaataaaaga aaacaaaaaa agcgaagact tatgtctcgc tttttttgtt ttgaatgaaa
    12121 aaaataaatt tttgctataa atcttttggt acatagaaac ttcggttgtc cataccgaaa
    12181 atccgatcgg tgtattttcc tggttctgtt tgacgaatcg aagtcaacat catctgcatc
    12241 gaagcatcga tattgtcgat ttgatgtaat atttctgctt ccattattct tggacgtaca
    12301 ggtgagccat attctaataa gccatggtgt gctaaaacca tatgacgtaa tacgagaaca
    12361 tcttcgtcag cttcgctgat tttcaacgca agacaggctt tagttatttc ttcgtcaatc
    12421 aataccagat gaccgatcaa atttcctgca agcgtatatt ctgtcgacat aggaccggaa
    12481 agttcaatca cctttcctaa atcatgcaaa atgataccag catacaacaa tgctcgattc
    12541 agctcaggat attcatcggc gattgatttc cctaagtgaa gcattgtcaa agtatgataa
    12601 gctaatccac tggcaaatgc atgatggttt ctttttgctg ccggaaactc aaaaaattgt
    12661 ttttgatatt tattaagtag atagcgaaca atacgattcc aatgcgcatt ggtgatctca
    12721 aacaacaatt gattgatttc ttcttccatt tcttctttct tcaaaggggc tctttccatg
    12781 tataaactag gctctcctgg ctcatctacc cttgttaagc gcatatgaag aatctttact
    12841 tgagggtttc cttgataaac ctctctttta ccgtttagaa aaacgactgt gccggctgta
    12901 aatttattga tttcctcctc cgaagcatcc cagtatttac catcgatagt acctgaagta
    12961 tcttggaaag taaaagcaat aaattttttt ccgtttttag cgattcttac atctgcactt
    13021 ttgatcagga caaattgttc aaactgctca tcgattacta aatcacgaat ttttttcatt
    13081 atttgctcct tctaattgtt ttacgatttg gttcatctca tggtaatagc ttaccatttc
    13141 tttatcagaa gaaaaacaaa ttatttgata cttttctcca aatttagcta aaagttcagc
    13201 taattgatac tttcgctgat gatcataatg aagccagcca tcatcaatga tgatcgggca
    13261 tatcgaacgg ttagcttcta atgagaggta agcaaatctc atcgccataa tcagctgatc
    13321 tttcgtacct gtagataact catagatggg aaattctgta tatccgtctg ttaaagtcag
    13381 tgtatcattt ttcagtaata tcttctgata atttcctttt gttaaaatac ctaaataatc
    13441 acctgctgct cttaacaact ggggtaattg tctttcactc atctctgttg aaagatcacc
    13501 taagaatgtc ccggcgattt caagtgctcc ccattcctcc agtaattcct gtaattttgc
    13561 tttttgccgg ctagcttgtt gataaagttc gtccaatgtg ccgtctcttt gcaactcttc
    13621 tacttgaagt tggagtcgtt gccgattttc acttgcttct ttttcttctt gctggagttg
    13681 ggattgcttt tctttcagtt ggcgcagtct tacagataat ttctcttttg tcagctcttc
    13741 aggaaatagt ggagtaagca tttccgataa ttcattgtat cttttcatct gctgttcggc
    13801 ttgtttggct ttttgtaaaa agacaggtac ttccgaagga taagataagc ctgcttgagt
    13861 caattgttcg ctgtattctt gtatccatac tttttgtttc tttttcaatt gattgatttc
    13921 ttgcttcaat aaagtatttt gctgatagct tctggcaaat ttcatctgtt ccatctcttg
    13981 tataaaacgg gaaagcaata ccattttttc tgaaattgtc ttatcttgta tcggtaacca
    14041 ttctttaaca aactcaaatt tttggtcgat ttgtcgtagt ttttcttgta tttcctgttc
    14101 tcttttatgt ttccgctcaa atacgccata taatttctga taatctatga cttcctgtcc
    14161 atattctttc atgagtttga gcgtatccat ctttcctaag tgccgatgat ccgcaaaacg
    14221 atatacttct tgctgaagaa tctgtttttc ttctccagtt ctcttttgtt tttcttctgc
    14281 ttcagctaat tgagctaagg ctagatcaag ttgtcctagt ttttcttgcc acattccttt
    14341 tatctcagag aaatcatttt tagttttaat ttttggttgc cttttgtaga agaaatagcc
    14401 aatcatgcct gcaccaatta gtaaaaggat cactttcaag ggcattggta caaatatacc
    14461 tgcaatgatt attgctccgc cagccatcag catatacggt ttagatgaag agactggtgc
    14521 tatctctgac tgattttgta ataatgctgg ataggtctct tccaagtcat tcacttctgc
    14581 ttcaatcatc tcttgctgtt ctttagctat ttggagacgc acagaaatct ggctttctag
    14641 atcagcaatc cttctttctt cagccagaag atgcttccat tcttcttcat ataaaaattc
    14701 ttccggtaac ggatttttct gccaatgcca cctttgttcc aaaatcttca attctgaatt
    14761 tgtttctcta gtctcaattt gtaatctttg ccattcttcg tttaattgtt ccgctgtaaa
    14821 ttgaagttgc tggagatctt ttagttcctg ctcctgctct aaataaaaat aatatctggc
    14881 tgactgctga tccatcccgc tatgcttttc cagtgttgct tctaattctt ccaaacgctc
    14941 atttgtttgc tgataccgtt tggagaaaga ctccagatct aaaagaagtt gctcatcagg
    15001 aagacctgta gtgctgtttt ttttgagaga ttgccattct tcatataaag aaaaattaag
    15061 ttcttgctgt ctggctaatg aaaattgttt gttcgcctct tttaattgtg ccaataactg
    15121 ctgctgttgc gactcgtatt cctgttcttg ttggatcaac ttgccaaaat aagcttcctg
    15181 ttcttgtttt tgatgaattt tttcttgcag ctctttccat tcatttaatt tttgattgat
    15241 tagtaatttt tggccttttt tcttgaataa ttgttgtgct ttctgataat attcttgccg
    15301 cttttggaat acttgagaac tgcccgtgat gcctaaagaa atcagcgcat catgtagctc
    15361 tttttctctc aaatgatcta gttccatcaa ctgctcttgc tggaaagtaa atacctgctg
    15421 gaataactct ttcgtcaaag gtgcgatcag tttttctagt aactcatcac ttcccacttg
    15481 atcccccagc cacaccttcg attttccgcg attttgctgc ttgtagcgct cgacagcaaa
    15541 ttcaccatat acaggatgtt taagccatat tttgccgcca taggctgagc cgtcttttgg
    15601 tgtataatcc ttttttttac ggccttttga agggaaacca aataatattg cttgaataaa
    15661 ttgataaatc gttgattttc ctgcttcgtt tcccccatag atcagctgat tgcctgaata
    15721 aaagtcaaaa cttttttgac gccattttcc aaaagcagca atctcgatcc ttaaaatctt
    15781 cattcctgat cctcctcaaa taaaaaatct tgattaagca gctgcttggc tttttcaagt
    15841 gtttcttctt gatattccgg cagttcgctc aatattctag ccgcttcttg atgactatat
    15901 acttcattta aaatttgttg gaaatcttga ggtgtctgat agtattgaaa taactgttcc
    15961 atcaatttag cagaagcagc aagtggggtc ttcgtataaa aagtattctc tatgagagaa
    16021 atacgccaaa taaagaaatc atttatccta tctgagaatt cctctaacaa atagtctaat
    16081 agctctcctg attgtatccg ttccaaaaca tctgtattga gatggtcata atcttttaat
    16141 tttatctcaa ttaaactaaa tccatcctct acttttgaaa gctgctgacg aatgtgaaca
    16201 atgacctctt ttgtcgttct tgctttcctc aatgatattt cctttgtctt ccaatagacc
    16261 tctgctactt taatcggatg tatttggcaa cttcctttac ttaattcaac tagtagaata
    16321 gatgtagaag cttgttcctt tttagtatga ccttgaggcg cacctggata aacaattgtt
    16381 tgcttttcat ttaagaccgt tggcacatgg atatgcccta aagcccaata atcgtatccc
    16441 ttttcttgca tctttgaggg ttggaaagga gcatagtttc ctttttgaga gacgccaggt
    16501 tccccgtggt acaacccaat atggtaatct gtgagttctc tagaaggaaa ttccatcact
    16561 ttgtcttttt gaatccacgg ttgacgataa ctaaatccac tgagagagac agtttctcca
    16621 ctttttatcg ttattttttt tgtctctacc gtttcggatg taaataaatg gacattttta
    16681 ggaaaggaga accaataccg ctctttttga tagaaatcat gatttccgaa tattataaat
    16741 acaggtatat ggttcttttc taaccgtttc atctgttctg aaaaatgttt ctgtattttt
    16801 aatgaaggac gattttgatg aaaattatct cctgcaagta aaacaaaatc aacgttattt
    16861 attattgcct gttcaatgat atttgataaa acggtcagat tcgtcttcaa tagtttttcc
    16921 tgtacagttt tatcaagagt tgtcagtcct tcaaatgacc gatccatatg taaatctgcc
    16981 gcatggataa atttcaccat taaaaatttc ctcctttttt tcttttatca taccattttc
    17041 aatgtttttt ttcatcaata accgaaaatt tgttcgtatt atgcaaagaa attacgataa
    17101 atggccgact tcaaaaaata agtttattcg aaaaaaagat tgtgacatga tttttgtcac
    17161 aatcctaaat atctatgtta tattcgcttc gtttgacact gaagaagtac tatttgtact
    17221 ggaatttatg ctccattttt ccgtatttag aattctatgt tctatactac cttaattagt
    17281 ttatatggta agaattcttt caaatgcttg agatactccg tctttatcat ttgaaacagt
    17341 gacataatca gcttttttca tgatttcttg atgactattt cccattgcaa aactaatatc
    17401 tgcactttca aacatagata agtcatttaa actatctccg atcgcgattc tcaaaggttt
    17461 ctttaaattg tagattgatt ccaaacgatc aaacaccaca cctttagata cattttgagg
    17521 tacgatttca atatttacag gtgccgattc agtaacagac acatttagca tctttttcaa
    17581 atggttaaat atattagctt tcaattgacg attaggaata aaaataaaaa atttgtaaat
    17641 gctgatagca tccttttcta taatttcctc aatattatta taagggattg aacaaataga
    17701 tttttcatac tcaagctcat agtttagctc tttgatagaa aattcactcc ctacctcgtg
    17761 aaaaattttt ttcattttat ttttataatt ctgagattca taacttccga gatttgtata
    17821 tattttggtt ggaaatttcg ataggacaga atcaattttt tttacatcat ttttatcgaa
    17881 ggaagactca aatatctttt tttctccatc aaatccaaca gaaccattta accctactgc
    17941 tggtatcctt aaaccagatt ttttgagaat attattaatg tcttctactt ctcttccaga
    18001 acaaatgaat ggaaaacagt cttccggtaa atgtttcatt gttcttgcat ttttttgact
    18061 aatgctctgg tcagaattca aaagagtacc gtctaaatct ataccaatta gtttcatttc
    18121 atccatgtct cctatacgtt gattataaat tcactgctta ttgtaagtct aaatttctca
    18181 caaaacatat actaattata cctaatttaa gtaagtacaa acatcatttt aagaatagga
    18241 aaggacattt atatactatt ctctgtattt ttgatttata aataaatatg aattgaatcc
    18301 aaatcagcta tattactatt ttataataac caactataaa aaacgaagtt aggtgaaaaa
    18361 ctaacttcga tattcataaa agactctatt agttcaactt agcaaaagat ttaaa
//
LOCUS       DAZABD010000050        18371 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000050 DAZABD010000000
VERSION     DAZABD010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18371
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.050"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..125
                     /locus_tag="ACHALG_001935"
     CDS             <1..125
                     /locus_tag="ACHALG_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFN2587316.1"
                     /translation="FFIQSVASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
     gene            complement(181..891)
                     /locus_tag="ACHALG_001936"
     CDS             complement(181..891)
                     /locus_tag="ACHALG_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HFN2587317.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGDESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(909..1405)
                     /locus_tag="ACHALG_001937"
                     /pseudo
     CDS             complement(909..1405)
                     /locus_tag="ACHALG_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(1442..1894)
                     /locus_tag="ACHALG_001938"
     CDS             complement(1442..1894)
                     /locus_tag="ACHALG_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFN2587318.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWSFVKKGNKRIY"
     gene            complement(2029..2634)
                     /locus_tag="ACHALG_001939"
     CDS             complement(2029..2634)
                     /locus_tag="ACHALG_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFN2587319.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(3084..3704)
                     /locus_tag="ACHALG_001940"
     CDS             complement(3084..3704)
                     /locus_tag="ACHALG_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFN2587320.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(3706..4575)
                     /locus_tag="ACHALG_001941"
     CDS             complement(3706..4575)
                     /locus_tag="ACHALG_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFN2587321.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANKAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(4598..5005)
                     /locus_tag="ACHALG_001942"
     CDS             complement(4598..5005)
                     /locus_tag="ACHALG_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFN2587322.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDRHKLAELYEQVGLKFSELHENDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(5024..6313)
                     /locus_tag="ACHALG_001943"
     CDS             complement(5024..6313)
                     /locus_tag="ACHALG_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFN2587323.1"
                     /translation="MSVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINDQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     ILLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(6340..6642)
                     /locus_tag="ACHALG_001944"
     CDS             complement(6340..6642)
                     /locus_tag="ACHALG_001944"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFN2587324.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(6667..8760)
                     /locus_tag="ACHALG_001945"
     CDS             complement(6667..8760)
                     /locus_tag="ACHALG_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFN2587325.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLEEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(9249..9977)
                     /locus_tag="ACHALG_001946"
     CDS             complement(9249..9977)
                     /locus_tag="ACHALG_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HFN2587326.1"
                     /translation="MGLCLFALPTQGFAVAGGHGGGTSSGRSSGRGSFGGRSYGGSYF
                     GSRSYGRDSYGYYGPSTGPSSYEQTGFLALAVLSVGGVSAFSIVKNKRQKKRLKSEIL
                     SRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFAEHQKVLQKNKAD
                     GVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLISGSKTQRQYFSQ
                     IWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(10122..10835)
                     /locus_tag="ACHALG_001947"
                     /pseudo
     CDS             complement(10122..10835)
                     /locus_tag="ACHALG_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
     gene            complement(10836..12743)
                     /locus_tag="ACHALG_001948"
                     /pseudo
     CDS             complement(10836..12743)
                     /locus_tag="ACHALG_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
     gene            complement(12857..14189)
                     /locus_tag="ACHALG_001949"
                     /pseudo
     CDS             complement(12857..14189)
                     /locus_tag="ACHALG_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            complement(14155..15633)
                     /locus_tag="ACHALG_001950"
     CDS             complement(14155..15633)
                     /locus_tag="ACHALG_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFN2587327.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLKNPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(15938..17773)
                     /gene="tdc"
                     /locus_tag="ACHALG_001951"
     CDS             complement(15938..17773)
                     /gene="tdc"
                     /locus_tag="ACHALG_001951"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFN2587328.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEQVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     EQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKEIAKAMEKKLNEIIQ"
     gene            18000..>18371
                     /locus_tag="ACHALG_001952"
     CDS             18000..>18371
                     /locus_tag="ACHALG_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFN2587329.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERPVS
                     CGMRTLYRLFSKGIFDIDTLPM"
BASE COUNT         5281 a         3906 c         3122 g         6062 t
ORIGIN      
        1 aattttttat tcaatctgtc gcatctaaac gaaataatat tcctagaaaa tcactggatt
       61 atcgaacacc tttggaagta tttttgagtt acgtaagtat tgatgatctg tctaacttaa
      121 tttgacaatt aagacaaaaa aaaaacggca atgaatgccg ctcttcttat ttcgattctt
      181 ttataattct gcttctgatt catctttggt tagtgtgtgt ctaacactag ttacgccaaa
      241 tgtttgatcg attttttcct tcaacgcttc gattgcctgt gcgccacgtg ccgcttgttc
      301 agcattgatg gattcaatga ccagcaccgc tgcttgtgtt tcttccgtca gcatcgttct
      361 ttgggcttca cgccagttga gacagcgaca gatcgctcct tgttcatcat agtagatgat
      421 ttcttccgga agtgccggag catcggaatc tgcacctaaa ggcaggaatg attcgtcacc
      481 ttgggctttt cctaaatgca gatttcctgc gatcttttcg atatcttctc cgccgcaagg
      541 tacgccaaaa gacaaggaaa tactattata gatatctaca agcggattga ttgggaaaaa
      601 ttcccgttct tgatttgcac gtttcagtaa agcttcaata gaagaacgtg cacctttttt
      661 agtcttgaat gtggtaaatg cctgacgcca ctgagcaatg acttcgtttt ggctgaatgt
      721 ttcttctgta aggtaatttc ttgcttcttt tgctgcctca ttcaataatt gatgatacgt
      781 ttcttcactt tcttttgtta cgtggttatc gataccattt acggataaga tttcgatggt
      841 tgcttcagga aatacttccc agaaattggt atccactatt acttttttca tctgttcaca
      901 cctcttgatt agaataattg cttgatgaat agatagtctg tttggggatc atctcccata
      961 taaaaaacat gttcgctgta tttgataaat ccttttttac gataaaaagc gagcgcaaca
     1021 tcgttgtgtt cccaaacacc gagccaaatg gctgtttttt gcttttcttt tgctttttca
     1081 atagctgtat ctagtaaaaa tgtaccgata ccttgatgtt taaatttttt tcgtacatag
     1141 attcgctcga tttctaaaaa atgatcagcc atttcctcag attgactcgt tcctgtattt
     1201 agtttcaggt aacccgcagg ctcttgatcg atactcacta ggaaaaaatc tgtttcttta
     1261 tcttttagct cttctgtaag tttttctagt gcataagctt gtgtcaagta atcagctaaa
     1321 tgctccggta attatctttt ccaaatgtat ctgtaaatgt ttcgatacta atttgttgta
     1381 gtaagtctac atctgaaggc gtcatttttc gaatttcgta tttattcacg gcttcttcct
     1441 ttcaatagat ccttttattg ccttttttga caaaactcca atcttcgcta acattcttgc
     1501 atactttatt taaaagatat tccaattgtt ttgcttcttc gtcagaaaga ccatttaatg
     1561 cgactgcatt ggagtagttg ttttctcgaa tgatgaaagg atagatttct gctccttttt
     1621 tagtcggata gagtttcttg atttttttat tttctttatc ttccaaacgt tcgatcatcc
     1681 cattggattc taatttcttg attgctcggg cagtcgttgt acgatctact ttgatcattt
     1741 ctgctagttt ttcttggatg attccaggat tttcgcaaat acgtactaaa taaagatact
     1801 gtcctcttgt taaatcgact tctttgaatt cgatattgct aatagagtct agcgctcggg
     1861 caatcattcc gatgtctcga aggatttcag ccatttcatc ccacctttat tttgtttgta
     1921 agactagttt acctcttttt gttgcaaatg caactatttt taataaaaaa atcaagttct
     1981 tttttaataa ctagtaaagt atattctttt cttcttttac gaaaaagctc aatcacaata
     2041 gatctggttt ttgttttcta aaataagtaa ctgtttcttc gttgttcgct tttctgatag
     2101 ctttggcaac tcttctatac taaaaaatcc catgttggaa gtttcattat tctcaataaa
     2161 cttggcctct tcaccgtgga tcgcacaagc aaaaacaagt ttataatact gaaaaagctg
     2221 tggaatatct ttttgtttat tcgtatcaaa aactgctcgc aactgagtag tttctactgt
     2281 taatcctgtt tcttcataga cttctttacg cacattttct tcaggagaca atcctatttc
     2341 agcaaatcca ccaggaagtg accattcttt cgtccgttta tcttcgatta gtaggacctt
     2401 gccatctttt ttgatatagg cacgaacatc tacttttggt gttggatgtc cttctccaat
     2461 cgttgtgagc gcttttattt cttcgaaagt ttcttttcct atttcactga tcagttccaa
     2521 tgaaatcgtc cggatctcct caaaacgctc tttatcaaat acatcttttc cataatatag
     2581 accagcttga gaaagtgcga gtagtctttt gtattgagcc aacttcttgt tcattctctt
     2641 cccttctttt ttagcaaatg tatgactatg taatcaagta tagaagactg gtttaattac
     2701 ggcaaataaa tataatgaaa tctgcaataa ataaaagaat gttaaaaaac tcgagaacaa
     2761 aagacattcg acttctctgg ggctgtgaat aaatggcgtt caaaagcttg catctgcggt
     2821 aataagcccc atcaaacgaa aaatatgacg agctattctt gtctggctgt tcttattaat
     2881 tgaggcagga cagctttttc acaacctccc cactaaacgg tgttctccta ccaactcctc
     2941 ggtcacaagt cacaatattc atcaaacatg agaagctgtt tctttgaaat tgctccttgt
     3001 gactcggagc tgaacgacag gctcacgacc tctcggtaaa cggtgttcaa aagccaattt
     3061 ttcgatatta agccatttct ttgttactta ttgtcattga tttctttttc taatttatcc
     3121 aatagtggag tgtaatccac ttgagtatag tctgttacat caaattcata tagtgttcct
     3181 aactggaatt tgtcatattt tcggtcggcg atgatctttt tgaatgcttc tttcgtaata
     3241 tgattggtcc ctttcgttcg atcttccaat acgtcaccat agtgataatg atccatgata
     3301 tagctcaaat gacgatctga ttcacgatca cgtttgtacc gacgttgcca taacacttca
     3361 aagtctgctg ttaggcgaat cgtgatcacc tgatatccgt atctatctgc taattcttgg
     3421 agtcgtgctt tttgcttatc actaaaagga tactcggaca caatcacttt ctttcctgct
     3481 tccatataca aatcaagtgc tttgtaatag aaaggataaa ctttcgtcct ttcttgttcc
     3541 gcccgttctt ccaaattgtt aaacccgatg gattctgcat agtattcctt gatttcatcg
     3601 agcgtgatgg catacatatc tggaaatcgt tcccgtagtt tattcataag atatgttttt
     3661 cctgtaccag gactaccagc cagaagaatg aaatactttt tcatctcatt tatttcctaa
     3721 gttaattgga ctttccaagt tcataaacag tccaatctta tgcttgacaa tttcttttac
     3781 tgctttattt gctggaatca cattatggcg aagagacttg ttgatttctg tttcactaaa
     3841 ggtttcttta gctactttcg tccagttgac taaaagctcc gttccaacat tgaatttgcg
     3901 gatcccgtta ttgatcaatt caggataatc ttcttctttc acacctgttc ctccatggat
     3961 aactaggggg acatcaacta ctgcatcgat ttctttcagc aagggaatgt ttacttctgt
     4021 ttttgatttg aattgtccat gattcgtacc gatagcaata gctaatgcat ccacgcctgt
     4081 agcttctaca aatttcactg cgtcttccgg tcttgtatat accttgtcat tttcagcgac
     4141 atggattcct tcttctgtac cgccgattgt tccaagttct ccttctacgg aaacaccgtg
     4201 cgcatgggca tattctacta ctgcttttgt cttcaagata ttttctttga aaggtagatg
     4261 ggacccgtca tacatgacag aagtatatcc gctatcgatt gcttctttga tatcatcaaa
     4321 gtctcgggcg tggtctaagt gtaagactgc atcaaccatt tcttcttctg caatagctgt
     4381 cattacattg actaacacat gcatgccgat atatttagcg gtatcgacac ttgtttgaat
     4441 aatgattggc gaacccattt cttttgctgc gcctagcatg gcaggaagca tttctaggtt
     4501 atgcgtatta aaagctccaa ctgtaaaatt caactcttct gctgtttttg ttacttcttt
     4561 caatgttgta tacatctttt attgccacct tatcttttta tttgtttcct ttgatcgtta
     4621 cttgttttgc aatttgtcgc atctcatcca ttttgcccat gtagtaatcg atcccttgat
     4681 catcaccagc acgagcagca tctgcacgct tctggttata gaggctcatc agttttttat
     4741 acccatctcc gatcgtctgt ttattttcca agctcttttc taaatattgg atggcttgat
     4801 cgttctcatg caattcagaa aatttaagtc caacttgttc ataaagttcc gctaatttat
     4861 gcctatcttg ggtagtggaa atctcttgtt tcaattgtgt aatctctgat tgaatctttt
     4921 cggtcgcttc tggcgatagg gctttttctt cttttgccag aacttttttc ggttcttttt
     4981 cttttttctt aaaaaatcca aacatcattt tccctacttt caattaaaac gctttcaaaa
     5041 ctgggcttaa gatccaggca tataagagtg ctgcggctac tgtatctaca tcggttgctg
     5101 tagcattgat gaatcccatc gaattaaaga tcgttactag caaagcgggc aataatgtaa
     5161 taaagaaacc atgagcaata ccgccaatga tcgctcctcg gcgtccgcca actgcgttgc
     5221 caaatatccc tgctgtccct ccggcaaaga aattggttag cataccaggt aaaatcatcg
     5281 ctaagccgaa catgggcaaa gtaaacatgg caataatcgt accaatcgtt gtcgtaatga
     5341 aacctaaaat gacggcattt gggctataag gaaagaaaac tggacaatct aaggcaggga
     5401 tcgcatcagg aactaatttc atagctatcc ctcgaaatgc cggcacgatt tcacctaaca
     5461 gtagacgtac accagctagc aatatataca cacccactac gaattggatg gcttgtaaaa
     5521 aggcaaacat gatatagttt tgatctcctg ataattcaga agcgaatggt tcgcctgcaa
     5581 ataaaacagt gatgatatat agcgggacca tcacgaccat gacagacaaa taagtatctt
     5641 gcagaaattc gaaatgtgtc ggcaacttga tatcttcgat cgacttttta ttttcgcctt
     5701 tctcaccaaa aagcttagca acaccggctt caaataagta cccgatcgta caaaaatgac
     5761 ctaaagcaat atcatttgat ccagtgattt tacgaatgat cggttgcgct actgccggca
     5821 tagcaatggc gaaacaagct ccgataaaac caccgaccaa gatcaatacg atccctcgca
     5881 gaccggcaaa atagccaaag actgtcgtca tcgttgccat ccataaaatt gcttgaccag
     5941 ttaaaaaaat gtacttccat tttgtgaaac gagccaaaat aatattgaat acaaaaatgg
     6001 ctaagaacgt cagtgcaata tctctaccta aacccaaatc attcattgct tgatcattga
     6061 tagattcaat agaagggatg atcccttgca tatgaaatcc ttcagtaaag attttcccga
     6121 aataagttaa acttccaacg atgatgctcg aaccggcact caatacttga aatcctagga
     6181 gtgtttttaa cgtgccagag atgacttgtc ctgctgattt cttctgcagt agtagaccca
     6241 acatagcaat caatgcaatg gtgatcgatg cctgagtcaa aatattttcg atgataaaat
     6301 tgataacgct catgttttta ccctcttttc tttttgtatt tagataactc ccttatcttg
     6361 cagaactggg gtgatttttt cttcaatttc attttttgaa acaatatttt tcaaatagat
     6421 catcgccgtt ttaggatcaa ctgcgaactt ttcgaactgc gaacgaaaat tttctgctgt
     6481 gatgatcaca tctggtttca tggaaactgc ggaagaaata tcacaatggt ccagtttggc
     6541 ttctactcct aatttgttca aaacatcctc agcagacatc tgtgctgcaa aactacttcc
     6601 taaacctgcg ccgcatacga ataaaatgtt caatttgttc atctttgttc tcccccgtat
     6661 catttattat ttctcaacta ctgaaaaaag cagttgtttg aaatcttcga tatttgtgca
     6721 ttcaatcaat cgatccaact tctcttcgtt gttgatcaat tcaattagtt ctttcatgat
     6781 gttcagatgg gagtaggagt ctactgctgc caaacaaaaa ataatcttta ctggatccat
     6841 ttcaggatta ccgaaatcga ttggttgctc aatcgtagct acgctgactc ctaatttatt
     6901 gaccccatct tctggtcggg catgggctag tgccatgtgt ttgccaatca cgatatacgg
     6961 tccgtaatca ttcactgcac gaatcatcgc ttcgacatag cgctcttcga caatgttttc
     7021 tttaaccaat ggtctagcag ctagttcaat cgattcctcc caagtagtgg ctttttcttt
     7081 gatcatgata ttgctgtccg tcaaaatatc ttttaacata ggctggatct cccttttgtt
     7141 gatttctaaa ttgtttgttt cgaataattt ttctaacttg ccatagatga ttcgatctac
     7201 tcggccacca ctttctttca tgacatctat tatttgagtg aataactccg tcgattcatg
     7261 actgttaggc tctaaccgct gtagttcttc atttcttttc aaaaagtctt ctataaccaa
     7321 gatattcgtc tcagttaaaa tcggatcgac aactaaaagc ggcttatttc gataattcaa
     7381 ttgaaaggtg ctgaaaacca gattgacatc caatttatct actaattcga tttcttttga
     7441 acttaaaact gcagtaatct cgatctgagg aaacttttct ttcagattct ctgctaacag
     7501 atttccagta gccatcccat ggttacagac aacgactgat ttataaacat aattcaagtc
     7561 tcgcttgatt acactggcga tcgtcgaaaa gtggatgaca agaaaagcta tctcgtcctc
     7621 cagtatcttg ccaccaatta cttcttcgat cgtaggaaca aaatgagtga ctgcgcggta
     7681 gattgggaaa taatttgttc ggatgttttc tttcaatgga ttcatgactt ggatatgatt
     7741 cttgattctt acaatcagtg cagccatgtg cttatagaga ttttcgaata aggcttcttc
     7801 ctttagatgg aaaggtacgt gtgtctcgtt ctctacaaat tggatcaatt ggatcgttag
     7861 taattgagct tgtacccact cgattgaatt ggagatatct ctagcattga acgtctcaat
     7921 agtgaaacga agataatttc tttctacttg cgggacattg tccaattgaa attcctcgat
     7981 catgtggtca ataaatgcag aaaagctact gtcttttatt tctgtatttt tccaattaat
     8041 ggcagagata aattcatgtt tcctgattcg ctggatccag atcaatgtaa aaagcaggat
     8101 ttgtttttta taaatatcat cttcttgttt caacaactta ttggaataga tctcctgtat
     8161 ttttttgatt tcctccaaag gaaaataatg gagaaagatt ttttgagccg gtgttaattg
     8221 tgcatcttga ttcataaaga agtcggtcat tcccagattc tgattgataa aatcatagat
     8281 catcgtacgg atagatcgtt ctgaaccttt ataaaccagt ccttgctttc cataactgac
     8341 gatctctatc ccataactga ccagtgttgc tcgtaaccgt ctcatatctt catctaatgt
     8401 acttttagag atttgaaatt cttcttcttt tttgtttaga tggatcggga ttcggctgta
     8461 agcaacggct aaaactagat caaaaatacg ttcgcgccga ttcaaaaagc cgtctttcac
     8521 atcttttatt tcagtttcta atcgagcttg cttttccttt tccagcttca acaaaaaacc
     8581 tttgttgcga attgtacgga tctccggaag ctttttactg ctcaaccaga tattcgcttc
     8641 aagaacttcc tgacgaatcg tccgctgact gacattgaac tcacttgcta gatgttttgc
     8701 ttttactgga tattgggctg tcatgattct gtgaagtatt tcaattgttc ttgttttcat
     8761 agaaatgatc tccttctgat ttcaagtata tggttccgct tacataataa aaagtagata
     8821 tttctgccac ttgaagtgga aagaaagtaa gcaaagcaaa caaaaaataa gccatatgct
     8881 ttttcatctc ctcaacgaaa aagacatacg cctattatta caaaaatttt gcccatctat
     8941 acaaaaaaga ggttgtgaaa aagctgtcct gcatcaagta ataagaacaa ctaaactaaa
     9001 atagtgcttc ctattttttg tttggttgtt cttattatcg cagatgcaag cttttaaaca
     9061 ccatttagtg gagaagctgt gacagagatg ttcagccttg agcgataaga agcgattttg
     9121 aaaaatcgct tcccatattt tacaaaatcg tgccttattg ccgataggct atctctggaa
     9181 caccgtttat tcagaaaaag gagctgcgac aaggcttttg tcacaactcc gttcataaat
     9241 atttcctgtc aatccagcat aatcggttga ataaaatcgg cttgccatct tttttctttc
     9301 tcagaaaaat caaaatacca tatctgagaa aaatactgtc gttgtgtctt acttcctgag
     9361 atcaatcgcc catcttttcg atcgactgaa taatcacagc atctgaaatc aattcggatg
     9421 gcaaaactat tttcgtggat ctgtcgataa tttcctactt cctgaatagt tattttttta
     9481 gtatgatttc ttacaccgtc tgctttgttt ttttgcagaa ctttctgatg ttcggcaaac
     9541 agcttgtctg tataacagct tctggctttt tcaggagttt cttcttccca agcggattgg
     9601 atcttcatga atatcttttg gatttccaaa aactgtgctt tcttttcttt agccgttcct
     9661 gacatgcggg acaatatctc cgatttcagc cttttctttt gtcttttatt cttgacgata
     9721 gaaaaagcag aaacccctcc tactgacaaa acagctaatg caagaaaccc cgtttgctca
     9781 taactagaag gacctgtact tggaccataa tacccataag aatctctgcc gtaagaacga
     9841 ctgccaaaat aactgccccc atatgaacga ccgccaaatg aacctctgcc cgaacttcga
     9901 ccgctagatg tacctccacc atgaccaccc gcgacagcga atccttgggt cggcagagca
     9961 aacaaacata ggcccactaa caaaatgatt gctaaataat tgattttttc attgtcttcc
    10021 tcgttatctt ttattacaaa aaactatttt atcatagcct tgctaaaagg aagagtcaat
    10081 aaccagatgc ataaactgtt tttttagcaa ttttgtctta tttagtcatt gagtaacatc
    10141 ggacagatgt atgtgtcggc agtccataat tcctctatct tcagcatgaa caagcttgca
    10201 gacaagagat ctgcgcttcc tccaggagag atttggtttt cctgacaaaa gtgcaacagt
    10261 gctgtaaatg gacgatttgg taaagcactt tgactagcag catgagcaag tttttgcatt
    10321 tccactaacg ttttctgatc actgcgatgc aatacgcatg tgtcatctag cgaactcatc
    10381 aaaactaaca acatatgcat ttgtttctgt acatatgaat cattatctag ctccttagct
    10441 gcttggattg caagcctgat atgcggatac cctaatatcg cttcttcata tgcaccattt
    10501 acgccatatt tttgcttcac cctatgacca tgagtggttt ttttatgacg atattttgta
    10561 tcaggaaaag cagcaagctt gctgacatca tcaagtattt tcgataaaga aaaaggctgt
    10621 tccatactga ttttttgtgc aatgacactg atcaatagcc ccattgccca aatcgctcct
    10681 ttatgggtat tgatgccatt tgtagcaatc agcattgcct tttccgcttt tcgcccaatc
    10741 tcagcaattc tttctcttaa ctcctgatca atagcataat gccatgctat ttgagccatt
    10801 tcttcaaaaa aaggagttag cgtcttagca gacttatcca aaatgcgccg accttctttt
    10861 acggcttttg cgatcgtatg gaaattatca tctcctaaaa ccatatcagc tgcctgtttg
    10921 cttacttcac tacctttgat tcccatagcg attcctatat cggctctttt caaagcaggt
    10981 gcatcattta cgccgtctcc ggtcattccg acgatttctc catttttctg caaagctttc
    11041 acgattcgca gtttgtgttc tggtgtcgtt cgagcaaaga cgtcgacttt tggtacttgt
    11101 cgaatcaact cttcttcaga cataagatcc aaatcgatcc cttctaaaac tttagcagta
    11161 tgttttaagc caatttgttc cgcgatcgct tttgctgtat ctttgtgatc ccccgtaatc
    11221 attttcactg agattcctgc ttgttggcat tcctttacag ctttgactgc actttctttt
    11281 ggcggatcaa tcaacccagc aatccctgca aaagttaatt gggttaaaga ctcatgcgtg
    11341 agttcctgtt tcgaatctac ttttttgtaa gcaaatccta aaacacgctg accttttttc
    11401 gcaaacgctg tggctgtttg accccatgct tcttattctg aagaagaaag agttgattta
    11461 gccaataata cttctggcgc acctttgacg taaatcaccc ctcctttttc ctccgcatgc
    11521 atcgttgcca tatatttgta ttcagagctg aacggaattt ttgcttctat ttttttcaaa
    11581 ggcagatcgg ctgtttcggt gtggcgaatc agtgcaagtt ctgtcggatt gccacttaaa
    11641 ttttctgttt tctgttcttc tttcgtcttt aattcctgac aatttttcat aataaacagt
    11701 acgcgagttt cttcctttgc tgctacatcc acaactgtca tttcattttt tgtcaatgtc
    11761 cctgtcttat ctgaacagat cactgtcata gaacctaatg tttctacaga aggcagtcct
    11821 ttgatgatcg ccttttcttt tgccatttca tgtactccga cagataggat catggtcaac
    11881 acagctggca atccttcggg taccattgac accaccaaag cgatcatagc tgaaagaagg
    11941 atatgccaat ccatgccata gcggaatgtt gtaaaaacaa ctaagaacaa gatcaaacaa
    12001 agaatgcctt gaaaaatttg tttgttcaat cggttgatct tcttgatcag aggcgtcgtt
    12061 tgttgttcga ccgattgcag tgcttgattg attttaccga tttctgtcgt gtttcctgtt
    12121 tctacaacga caccgactgc agaaccagat tgtacaagcg ttcctgaaaa agccatattt
    12181 ttttggtcac cgattgattc ttctgctgcc aaaacggaat gatttttttc agatgcttct
    12241 gattctcccg tcaaaatcga ttcttctact agtagatcat gtacttcgat caaccgaaca
    12301 tctgctggaa cgacgtctcc ggcacgtaag acaatgacat ctccttttac tagttctttc
    12361 gcagggattt tttcttgcat cccgttcgat agtactatcg cttcttgtcc catcagctgc
    12421 ttcaaaccat tcaacgattc ttctgctttt ctttcttgcc aataactaac gagtccgttg
    12481 acgatcacta cgaataaaat aatacttcct tcgatgtatt cgctcgaagc aaatttcaaa
    12541 aaagaggcaa tgatcaacac gatcatcagc agatcagtaa agtgttttgc tatttttttc
    12601 catcgttttg tctgctgtct tgcttctatc tcattatatc cttgatcagt caggcgtttc
    12661 tgccgttctt cttgcgacaa gccttctgtt gatgtttgat agtttttcaa tacatcttct
    12721 accgtcaatc tgtaccagtt catcctatcc cctcctgttt aattgatact gttattatac
    12781 gcagggaagt tttttcaggc gtatcgatta tttcaggggg gtctgaaata attcgttttg
    12841 cagaatgtat ctgggcctaa ctgatatttc tattgcttat tcttttatat acaaaattta
    12901 agaatacaaa ccagataacc aataaaaaag gaataaagcg tgtcgtgctt tcgaataaca
    12961 agctgaaagc aattgaagta aaaaacacca acaaagcaag atctttatat ctttgtgtag
    13021 tgttccgttt cttttcttta ataaattgaa catgtgcaca caagatcgaa gaccacacaa
    13081 atagaaaaca actcgttgtc atgcctgcca aaagctgaaa aaacaatcga ctatcagcta
    13141 caaaaaaaat gtagacaaag accgacaaaa aataacatcc ctgtaattaa aataccgtgt
    13201 aaaggtacgc ctttcttaga aagcgcacaa aaccaatggg gcaaactttt ctcttttgct
    13261 gtttgatata aaatcctgct agtactgtag actgcactat ttgctgagga caaagaagca
    13321 accaaaatca ttagattgac aattgctgct ccgctttggc aatggatcac ttgcagcata
    13381 gtcactaaag gactttcttt taaatctaaa caatgccatg gaacaacaaa taaaatcgac
    13441 aaaatcgttc ctatatagaa aaagaggata cgccaaggca gacagttgat tgcatttttc
    13501 aaattctttt gtgggtcact cgtctcacca actgttaatc cgatcatttc aattccagca
    13561 aaactgaata aaaccatagg aaatgaagca atcattccag caaagccaaa cgggactaat
    13621 tcattgagct gtccaaaatt tgctttgatt ccaatagact gggtatgaaa atctgagaca
    13681 ctcaatccta tacccaataa gataaaaagg aaaatcccca agatcttgat gattgagaaa
    13741 aaagattcca attcaccaaa ccacttgact gcaacaaggt tgactgccat taagatcatt
    13801 agtgcgctac ctccgcttat caaagctggt acatgtacaa accagaaatg gatatacaat
    13861 cctattgctg ttaattccgc aagtccagct ccaagccagc aaagccaata agtccaattg
    13921 acaaaaaatg cccacttttt gcctaaataa tagtcaacga aatcactgat agagttaaat
    13981 gtactatttg ctaaaaataa atcacccatg acccgcatca aaccataaat agccaaaccg
    14041 catatcaaat aaacgagaat aacagctggc cctgctgatg caattgcctt tgctgaacct
    14101 aaaaataatc ctgttccaat tgcgccacct aatgcgatta actggatatt tcgattagtt
    14161 agagtccgtt tcattgccgg tttttccatc aagttcacct ttttcttttt caagcagcat
    14221 ggcgacttcc tgttcccaag aaggtttctt tattttttca atacagagtg gtactacaaa
    14281 caaaatgaca ctgatggctg tgactgcaat tagattgatc catgtcccag ctttcatcgt
    14341 cagtccgaca gaaaatccga ttgccgcaaa taaaacaata acgacagtcc aaactccaag
    14401 attgctcttt ttctcttttt ttccaattct aaatggtcga ttcaactttg catgtgtata
    14461 acgcaatctc aaaactgcga tccccatgat gacatagacg atacagtaga taacagtagt
    14521 tgaatttacg agcatcaaaa atgcttgatt gacgccagga atcagcaaat aaaaaagtgc
    14581 aaagattgag ataatgacag actgagtcaa aataacggat ttggatacgt caaattgatt
    14641 cgtcttccaa aaattgaatt ttggtggata cgcacctttt ctagcacttt ctgtcactgt
    14701 ctttgctggt cctgaggccc aagcagataa ttggactgct acaccaatga agacaagtaa
    14761 actaaacaga ttcacaataa gatgtggcaa accaagaatt tggcaataaa tcgaaataga
    14821 ttgcgcgata ttattcaatt ccatttgtcc ttttggtacg acatttgcta caactaaagc
    14881 attcaaaata ttgacgaaaa atataaaaat taacgcggca aatactccgc gaatatacgt
    14941 tttaactgga tttttcaacc gagtgatata cacagaggac atttcgatac cagtaaaaat
    15001 aaacatgatc ggcgcaaaat aaacaaatga ggatgcagtc gtagtatctg gtattaattt
    15061 gtcccatgaa aaactgccta atgttccggt tggtacgatc ccaactttga tccacgcagc
    15121 aaaccctaat aagagcatca tcgtcaaagg cacgtacaat cctaaccaaa caccgacttt
    15181 tccgccgatt tttgccatat caaatttcaa attcaaaaaa gtgatgatcc aataaacgac
    15241 taaaatgaca actaatgtaa atttgctgtt caaaccaagc ggcacattat cgataatatt
    15301 accaaacaac ggagcaagtg ctgaagcgac catcaccatc ccaggaaaca tttggaccca
    15361 taacagccat gagacaacga acccccattt ttttcctaga gcattcgata cccaaagttc
    15421 tggaccacct ttttggggaa acattccggc aaattcacca gagatcaatg agattggaaa
    15481 agcgtacaat aatactgcaa ccagcatgta aaaaatcatc gtccagctcg ttgctgctac
    15541 atctggaata tttcgtacac ttgctacaag ggcgcaggtc attccgataa aggtcatggt
    15601 cgtgatttgc tttgatgaac cagtatcttt catctttatt ccttctttca atagcgacat
    15661 atgtttttgt tttcttttat ttttgttcaa gaacagaaat gacaaggttt catcgattca
    15721 gtacaaaacc cagtatcacc gaaacatcca ttgaacgatc ttagtgaact gggtttgata
    15781 catgaaacga acgatccaac ttatagaacg ttttataaaa aagaatatct tggcgaggaa
    15841 tatatgaaaa acagggggaa atacaattgt tttgatgaag caaataattg tctgttctcc
    15901 atatgtctct tgttattctt tcacacaaaa atcagttcta ttggatgatt tcgtttagtt
    15961 tcttttccat tgctttggcg atttcttttg tataaaaatt gaaaattcga tcatcattca
    16021 aataaggagt catgatagcc gcacgtagca gagtgatttt ctgttctttt ttccattctt
    16081 ctttcgttag acccattctt tcaacaaaac gaatcggaga atcaccgtaa tcttcttgtg
    16141 taaatgtagt atgagaagta ataaaccgtt caccataaac atcgccatcc atataagaag
    16201 accgatcgaa cattttttca tttaattcat tgatagcgtt caaatctgta cagccttgtt
    16261 ctttgaatac ccaattgacc atattaaaat caggatgatt caatggatag acttcgattg
    16321 tttttccttt aacagtaaaa gtcagatgat cgagaaattc tctgaatctt tgagctgcct
    16381 cgattgaagc cccaatcaat tgaccatatc cggtcacatt caatggcaat acacgatgtg
    16441 ctgtccagac tgcggcggcg gttgctccag cttttgatcc ttccaaaata taagctccta
    16501 acatatctgg agcctttact gatttttcaa acacatatgg cgcgaaataa gaaatgatat
    16561 ttctcatatt tttatgtttg attacgattc ctccagcagc ataagggaca tagcccatct
    16621 tatgaggatc tatcgtgaca gaatccgctt ctgaaatagc tctgaaccct tcatatacct
    16681 ctttgtcaat cgttacgcag tggtggaaaa catgatgttc ttcaaagaat tcagctaagg
    16741 aagcatacgg cacaaattct cctgcttcgt ttagaaacag tgaacgcgca tagccgccat
    16801 atgcggcatc aacatgtaaa tagaaataga tcccttcatc ttttaacctt tctcttaatt
    16861 ggacgatttt atctacactg tctacttgac cttcttctgt cgttcctacg acagcaacca
    16921 cacctagaat tgggattttt tgttcagcta attcacgaat tgttttttcc aaagcattaa
    16981 tatccattcg ataatcctct tgaactggga tagcaaccat ctgatctaag cctactccgc
    17041 aaatatctaa cgccttcatc caagaatagt gttttgtttg aggcaccagc catttcccta
    17101 atctttggat attttttccg ctacgggaag aagctgcttt gacctcatcc atttcttcgt
    17161 ccgtaaagct ttcggtcatt tccaaaattt cttcaacaga taaattcaaa agttcccact
    17221 cagacatttt tttcacttgt tcaggcagca cttccttgac agctaaagga atcgatttga
    17281 tgcagcgggc gtaccataat ccttcaagat tagcaataga accatctgct gcaatgtgtc
    17341 cccatccgtc tgtcatattg aataatgaag caaaatcctg tcctacttct gcttccattt
    17401 gcgaagttgc catagaagat tccaatgcaa cattatttgg gttccacagc atcgcatagt
    17461 tgtaagctaa taaagcaggc atcaatgttt cagcattcat ttgtccccag taacggcctg
    17521 ctgaatgcca tgggatcgat cctgcgcgta atcgctgact gacttcatcc aagacctcta
    17581 tcatatggtc gcgggtggct agataatctg gagtgagctt atctccttcg ctaattgcag
    17641 gcatatctga aggaatataa ttttctctcc atccaagatg ttcatctacc atcttgttca
    17701 acaacatttt ataaactggt ccattttcag ctttgtcgcc tataaagacg gccttgatat
    17761 ccatatcttt catttttctt ccctccatat tttgttggtc aatgtctcgc agatacatgt
    17821 gtttatctgt aacacctctt cactatagcg ccaaaaatga gaaatttttt tttataacat
    17881 caaatccacg tagattttta caacgcgaat tgtaaaatta agttagaaaa ataaaaaggc
    17941 atttatggta cactcaaatt gtatttccga cgaaagaaaa caaaggagtg taaccataaa
    18001 tgacctacaa acatcttacc atagacgaac tgacaatgat agaatcatat tatcttcaac
    18061 ataataagcc ggttgaaatc gctaaccgaa tgggacgtgc tatacaaact atttataatg
    18121 tagtcaataa gttcaagcaa ggcaagactg ctcttgatta ttggcaccag tataaagaaa
    18181 ataagaaaaa atgtggtaga aaagtcattc aattacctgc tcatgaagta gattacatta
    18241 aagagaaagt cactcttggt tggacgcctg acgtcattat cgggcgaaaa gaaaggcctg
    18301 tttcatgcgg tatgagaaca ctttatcgtt tattttctaa aggaatattt gatattgaca
    18361 cactaccgat g
//
LOCUS       DAZABD010000051        18125 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000051 DAZABD010000000
VERSION     DAZABD010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18125
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.051"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(24..722)
                     /locus_tag="ACHALG_001953"
     CDS             complement(24..722)
                     /locus_tag="ACHALG_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HFN2587330.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(715..2067)
                     /locus_tag="ACHALG_001954"
     CDS             complement(715..2067)
                     /locus_tag="ACHALG_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HFN2587331.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2290..3690)
                     /locus_tag="ACHALG_001955"
     CDS             complement(2290..3690)
                     /locus_tag="ACHALG_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFN2587332.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3851..4588
                     /locus_tag="ACHALG_001956"
     CDS             3851..4588
                     /locus_tag="ACHALG_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFN2587333.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4713..5192
                     /locus_tag="ACHALG_001957"
     CDS             4713..5192
                     /locus_tag="ACHALG_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HFN2587334.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5189..5956
                     /locus_tag="ACHALG_001958"
     CDS             5189..5956
                     /locus_tag="ACHALG_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6681 family protein"
                     /protein_id="HFN2587335.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6186..6755
                     /gene="lepB"
                     /locus_tag="ACHALG_001959"
     CDS             6186..6755
                     /gene="lepB"
                     /locus_tag="ACHALG_001959"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFN2587336.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6859..7461)
                     /locus_tag="ACHALG_001960"
     CDS             complement(6859..7461)
                     /locus_tag="ACHALG_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFN2587337.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7567..7797
                     /locus_tag="ACHALG_001961"
     CDS             7567..7797
                     /locus_tag="ACHALG_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587338.1"
                     /translation="MPISFVKDREEKGKCVREILLDLPEWFGLPESTEKYIEESSKLP
                     LWCEKRKEEYLGFITLSQTSEDTAEIYSIVWE"
     gene            7788..8036
                     /locus_tag="ACHALG_001962"
     CDS             7788..8036
                     /locus_tag="ACHALG_001962"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN2587339.1"
                     /translation="MGIKKDYHHQGLGTKLFKEAEGYAAKHYKYLQVKTVDEGHYSIY
                     DQTICFYESLGFSRLEVFPNLWDEWNPCLVLVKKLEQK"
     gene            8142..8726
                     /locus_tag="ACHALG_001963"
     CDS             8142..8726
                     /locus_tag="ACHALG_001963"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFN2587340.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8729..9802
                     /gene="prfA"
                     /locus_tag="ACHALG_001964"
     CDS             8729..9802
                     /gene="prfA"
                     /locus_tag="ACHALG_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFN2587341.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9795..10634
                     /gene="prmC"
                     /locus_tag="ACHALG_001965"
     CDS             9795..10634
                     /gene="prmC"
                     /locus_tag="ACHALG_001965"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFN2587342.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10627..10806
                     /locus_tag="ACHALG_001966"
     CDS             10627..10806
                     /locus_tag="ACHALG_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587343.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10828..11853
                     /locus_tag="ACHALG_001967"
     CDS             10828..11853
                     /locus_tag="ACHALG_001967"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFN2587344.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12028..13272
                     /gene="glyA"
                     /locus_tag="ACHALG_001968"
     CDS             12028..13272
                     /gene="glyA"
                     /locus_tag="ACHALG_001968"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFN2587345.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13425..14372
                     /locus_tag="ACHALG_001969"
     CDS             13425..14372
                     /locus_tag="ACHALG_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HFN2587346.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14506..15135
                     /gene="upp"
                     /locus_tag="ACHALG_001970"
     CDS             14506..15135
                     /gene="upp"
                     /locus_tag="ACHALG_001970"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFN2587347.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15274..15516)
                     /locus_tag="ACHALG_001971"
     CDS             complement(15274..15516)
                     /locus_tag="ACHALG_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587348.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15637..15709
                     /locus_tag="ACHALG_001972"
     tRNA            15637..15709
                     /locus_tag="ACHALG_001972"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15670..15672,aa:Thr,seq:cgt)
     gene            complement(15828..16466)
                     /locus_tag="ACHALG_001973"
     CDS             complement(15828..16466)
                     /locus_tag="ACHALG_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HFN2587349.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(16589..17221)
                     /locus_tag="ACHALG_001974"
     CDS             complement(16589..17221)
                     /locus_tag="ACHALG_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFN2587350.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            17572..17871
                     /locus_tag="ACHALG_001975"
     CDS             17572..17871
                     /locus_tag="ACHALG_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFN2587351.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT         6215 a         3162 c         3571 g         5177 t
ORIGIN      
        1 tcacattccc ctttttcgaa taactaggct gtttgtcctt caagataggg cagatccaat
       61 tctttaccgt aacgttgttc taaagctagg cggatcaaac gagctgccag gttctcattt
      121 cttgcaagga taggtccatg gaaataagaa ccgaagacat ttcggtaaat gacaccttca
      181 gattggtctt caccattatt tccttgacct tgtaccactt tccccaaagg tcgttcacct
      241 tcacctaaga aagtccgacc attgtggttt tcaaatccat aataagtttc attgaattct
      301 tcgttatgga taacgatatc accgataaac cggttatttt cctgacttaa cgtgtagtga
      361 tccagtgcac tgattccttg gatcttttct ccattagctc ccatgtagta gtgacctagc
      421 aattggtagc caccacaaat ggctaacatg acgccatcat tttcgatata gttgatcaaa
      481 gaatctttct ttttctggat gtcttttgaa atgattaatt gctcaaagtc ttgaccaccg
      541 ccaaagaaaa ccaagtcata tttatcagca tcaaaaggtt catgtatact aacgatctcc
      601 gtatggcaag aaacccccat tttttctgct atatatttca acatcaaaag gtttccgtta
      661 tcaccatatg tgttcatcaa atttccataa aggtgggcga tattgagttc ataattagcc
      721 acggttcatt cctcctttga taaatccttg agcagctagt tctttacgaa gctgcaatac
      781 agcagtgtaa gtagctaaaa tatagacatg ctcagttggc aattctttga ttttttcgat
      841 tacttctggt aattcttttg tttctgtcaa tttattttct ggaatcccgg caacttttag
      901 acgtaaagtc atatctgtat gacggtctcc tccagaaata acagctggga tatccatatc
      961 ggcaaagttt tcaaaattac catcccaaat ccagctgaca tcgattccgt ctgcgtaatt
     1021 ggcatttaat aaagccacta aagaaaatgg ataaggagct agaccaatca tatcgattac
     1081 ttgattgata cctacaggat ttttgacaag tactaatgta cattttttcc cattgatatt
     1141 gatgacttct tgtcggccga atactttctc gtcatatcct aaaccttgtt tgattttatc
     1201 aggatcgaca ttgaagtact ctgccactgc agtagctgct aaagcattgt agacattata
     1261 cataccgcca acttcgattc cgtattcatg accatcgatc acgaaatctg ctgacacatt
     1321 gtccatccgt accatatcag ttaatcgata atccaattca ggacgtttga agtcacaatt
     1381 tggacagtaa tatttcccta agtttgcata agtgatcatt ttataatgca agatgtgatg
     1441 acaatttgga cataagacac cgtctgtatt gtaatgagcc atttgttcac gatcttcttc
     1501 atggtcaaag ccataatatt ttctaggatt gactgtttcg atagaattga agattggtga
     1561 atcaccgtta ctgataatcg ttgcatttgg tgactttgcg gcaccgtcta caatcatttt
     1621 ataggtcgta tagatttctc cataccgatc catttgatca cggaaaatat ttgtaaataa
     1681 gaacagttct ggtttcatat attctgtgac tttgctcaaa cttgcttcat ctatttctag
     1741 gacagcaaac tttttctgtc ctttttttgg ttttgcctgc aagaaagtcg atacaattcc
     1801 ctgtaccata ttcgcaccag tagggttggt tagaacttca tcgaattgct ggcgcaaaat
     1861 attgactgtt aaagcggtag tcaatgtttt accgtttgtt ccggtgacaa cgacgacttg
     1921 gtagtctttt gccaaactat ctagtatatg tggatcgact ttcagcgcta gtttacctgg
     1981 taaacttgat ccgcctttaa aaaaagtttt taatgcccat tgcgacgttt ttccaactgc
     2041 aatcgcaaaa tgactgcgta ttcccattat gtaacctcca aaaacgattt gctgacgaat
     2101 aaattcaact aatcatacca tatgttgaaa aaaaggttaa attgcttttc ttattcttaa
     2161 gaaaaggtta cggctgaaag tgcatgaaat cggacaaaat cgcatgattt aacaaaaaaa
     2221 cagcttgaaa caaaatgatt gtttcaagct gctgtgattt tattattccc tcttacctat
     2281 attcccattt taaccgataa taattttctc gcttggatat tcatagccta gatctcgtgt
     2341 ttcttttggc acgaataaca tcaatgtata tagcattccg atccgcccaa taaacatcag
     2401 tgtagcaatc gtgattttgc ccacaacaga caagtctccg gtaatcccta aagataaacc
     2461 agttgttcca aacgcagaag ttacttcgat gatgatcgag atcagcgttt gttcttctgt
     2521 agcagataaa aaaacgatac aaaagaaaca cataccaaga gaaagcataa agacaacgac
     2581 tgattttcgc acatcatctt gatcgattct ccgtccaaaa atattgatgt tatcttcact
     2641 tttgaggaag gaatatagat acaaaccgat gattgctaca gtagttgttc gaatcccgcc
     2701 gcctacagaa ctaggactgc atccgataaa catcagtaaa gaaaaaatga tcagtgttgt
     2761 gatttgaaaa tcacccaaat cgtgaatctg cagaccagca ttacgagtcg taatcgaata
     2821 aaacatcgaa ttgatccatt tgacactcat attggaatct tggaataaat ggtccttttc
     2881 caatagataa atcaagacag ttcccgaaac aaacaagata acaaaagcta acacagctaa
     2941 tttggtaaac aaggaaaaac gaaacggaag cttcgcattg gaacgtttgt ataatagcca
     3001 ttcccggcac tccatcaata caggaaaacc aattcctccg acaaaaatca aaaacatgat
     3061 caggatcaag aaaaaatagt catgtgcaaa gggcttgatg gaatccccag tgacgtcaaa
     3121 cccagagttc gtgacagccg atatcgcctg ataaaaaccg taaaaaacgg catcacgcca
     3181 acgatcaaaa tagcctctgt aataaaaata aatagaaaag aatgtcccaa aaagtaattg
     3241 aaaccagatt aaaatagcaa aagtgatccg aatcagtcgg acgataccag acaaacgcgg
     3301 ttgattcata tccgtcatga tcagctgtcg ctgtttcagt gtgatccgtc gtttagaaaa
     3361 aatgacaaaa gcagtagaaa tcatcatgat ccccaaacca ccaacttgaa ataatatttc
     3421 cagcagtata ataccgttat cattgaacac agaattgata tcaaaagtcg atagtccagt
     3481 cacactcact gtactgattg ccatgaacag catgtccaca aaagaaacat ggctatctgg
     3541 ttcgcgaaaa atcggcatat aaaatagaac gagtgataag accgtcatca atatataata
     3601 aaaaacgatg atttggatcg atgagaaatg attggcagca aacttctgtc ctcgccgctg
     3661 cagccttcga aagaataaat caacatgcac agtactatcc tcctgacaat agtcgtttct
     3721 accagtatac aaggggaatt aggaatactc aaagaaatta tcaaacagat aacaatacag
     3781 ctttcgatta aaatagaaat ggcttataat aaggaaaatc ctagaaaaaa gaaggaggag
     3841 gaataacaaa ttgattaatg ctcaacaaat cgtagaaaaa atgaaaaatc aaaaaataaa
     3901 ttatgataca gttctgaaaa aaatgatcat gcaatgggag aaagaagaag taaagcctaa
     3961 gatattaata catacatgct gtgctccttg cagtacgcac acattggaat tcatgtctca
     4021 atatgcagag atcacgttat ttttttcaaa ttcgaatatt catcctaaaa gtgagtattt
     4081 gcgaagatta catgaacaaa aacgatttgt agaccaattt aatgagaaaa ctgaccatca
     4141 tgttgcgcta atcgttgatg aataccgtcc aaatgaattc accaaaatgg ttttaacaaa
     4201 ccatcttgaa aacgaaaaag aaggtggtgc tcgctgcagt gcatgcttca atatgcgatt
     4261 agatcttgtt gcgcaaaaag cgcaagaact aggatacgat tactttggaa gtgccctgac
     4321 catctcgcca aagaaaaatg cagctttgat caatcagatc ggaatggata tccaaaagat
     4381 ttacgggaca caatacttgc caagtgattt caagaaaaac aaaggctatg aacgctcttt
     4441 gcaaatgtgc aaagagtatg atatttatcg ccaatgttat tgtggttgcg tgttcgctgc
     4501 caagcagcaa ggatgcgatc tgaaagaaat caatcgggaa gcaaaagctt acttaaaaac
     4561 atcaaccgta gtttttgagg aaaattaaag aaatctttcg gaaaaacgta agaaaaaaat
     4621 aaagaattta tggtagaaat aaggtagact gtataatttg tccgtagtaa agtaaggaaa
     4681 caagaataaa tttcaatgaa ggaagagata agatgcgaaa agtagcacct attttgaagc
     4741 caatcgctgt attaacaatc tcgacgattg tcgcgactac tacaggtatt gtgaatagtt
     4801 ccagctaccg tgcaccaagt tcaagttcta gtactgtaga agaaacaaca ccaactactt
     4861 cagtctcgac atctgaacca acaacaacgg aaagtttgaa tgaaaccact acgacaagta
     4921 catgggacga tacaacaact agttcgtcgt atgatgagac atatacaact gacagctcaa
     4981 gcgaaacaag taatacggta ccagatggca ctccatcgga ttcaaccaat ccaagtacaa
     5041 gtccggatac aagtgctagc tccgaaacga taccaagcag tacgactgta gatgataaca
     5101 cgcaaaatga tgatgcagca cgtacagcac agcaaaacca agattcagga aatacccaaa
     5161 cgtataacca gcaagcaggt gattctcaat gacattttta ctagacctta tcaacggggt
     5221 gcataaattc cttggctatt tagacatcag tcctaaatat cttaaccgtg gatatacgat
     5281 tttaagtgtg attcccacgc tttatttatt gagaattgtt tatggacttt ggcaaaacca
     5341 aaactatctt caatttttct tatatggact tgcatttcta gtgcttgttt attttactgt
     5401 tttaaatgta ttttattact ttttagataa aaacacgaaa gccgatgtca ctcagctttt
     5461 tgtgaagtat ttacctgatg aagcgttcaa catccagaca gaagaatcga tagataatgg
     5521 aaattcgatc gataaagtga atacacaaga ggtcatggtt tcttatgacg aagattatca
     5581 attgaaatta gccgagaata tgcggtattt gattggaaat ggtgagatca aaacaaatga
     5641 actcggtcgc atggatggat ttctggtcga tagaaataca ttgtatcctt attactatgt
     5701 caagaaaaca ggagagaaag agtatgttct aaatattgga cgaagctact cggacatgga
     5761 aaaagtaggg accattcgac ttgactcaac agaagacaca ttggaacctg tcggattgtt
     5821 catcgtcggc ggtgattttg tcaaagacgg gttacgctat catgagcctt atcgtctgaa
     5881 attgattgca aaaaaggcac agtcgcagga agaaccagtg gtttatagtc gtagccaaag
     5941 aagaagacat tcataatgtg atgaaacgcc gtgtatcagt ggttgcgata aaaacgttta
     6001 ttcggaataa gggggatgtg acacgtcttt tgtcacgttc cttttttata taacgagaaa
     6061 gataagacga acacatcact tattctttcc tttcatttat aaacattgta gaataaagaa
     6121 agtacaacga agtattgtga aaaaacgtaa taaataacga ggaaaataca agtaggaggt
     6181 aagctgtgaa agaatttttg aaaaattggg ggattttgat ccttgtatta gcggctattt
     6241 tattagctag agtgtacgtg tttacaccag ttactgtaaa cgggcattcg atggacccta
     6301 cattaagtga tggacaacga ttgatttcat ctaagatctc aaactatgaa cgaatggata
     6361 ttattactac aaaggaaccg ggggatgaag aacggatgat tgttaaacgg atcatcggta
     6421 tgcctggaga tacagtcaag atggaaaatg accagttgac cattaatggc aaaaagtatg
     6481 atgaacctta tctggatgag ttcaaaaaag aattttcaga agataagtta caaggagaat
     6541 atgcttatag ctcaggattc caagcacaag cagaaagttc atccaccttt acgagtgact
     6601 ttgaatatac tgtgccaaaa ggaaagtatc ttgttctagg cgataaccgc ttgatttcaa
     6661 aagacagccg aatgtttggg ttagtcgaca aagatatgat ccaaggaaaa gttgttttcc
     6721 gttattggcc tctttcagaa attaaaatca tgtgatgaaa aaataaaaag aagctgtgac
     6781 atgttttttg tcacagcttc tttttccgaa aattccttct tatttcttgg agttgctcac
     6841 tttttttgca atctcatttt aagctttttg gctgatggat aagaaaagcg aagcgttttt
     6901 gttcataaat agcttttccc cacgatggaa ggtatgacta gtagcagaga caaagcccgt
     6961 tttttccaat cgattattca attcttcttg gctaaagcca ttatggactt tcggatgact
     7021 aatttgttca ttcttgtcaa agtctacgat gatcaattga ccgccaggag ctaaaatttc
     7081 ataaagtttt gtaagaatat tctccgtatc aggaatgtgt aaaagtacaa gtgatagaag
     7141 gatgagatta gcttggacag caggaaattc gacagaagca tcagcgtgga tcgttcggac
     7201 atttttcaga tctgctgctt ggatcttttc ttctgccatt ttcagcatag tttctgaagc
     7261 atcggcaatg atcagttctt tgaatctttc cgcaagcggt aaactaacaa gcccagttcc
     7321 gcctccgtaa tccagcagga ctttttggtg gctgtcgcgc ggtagataag tagttagttc
     7381 ttcgcgaata atcttggcta atgcttgacg gtcaggggaa tcataatggt tagcgatgga
     7441 atcaaaaata ttttttgtca tcaaatagct ccttacgtaa aactttgaaa ttttaactta
     7501 ttatagcacg tgaaaagaaa gaaagcatgc tatactcaat tcaaaaaggg agaatctaga
     7561 aagaatatgc cgatatcgtt tgtaaaagat agagaggaaa aaggaaagtg tgtccgagaa
     7621 atattactag acttgcctga gtggtttggg cttcctgaat caacggaaaa atatatcgag
     7681 gaatcaagta aactaccttt atggtgtgaa aaaagaaaag aagaatattt aggctttatc
     7741 actctttcac agacaagtga agatactgcc gaaatctatt ctattgtatg ggaataaaaa
     7801 aagactatca tcaccaagga ttaggaacaa aactttttaa agaagcagaa ggttacgcag
     7861 caaaacatta caaatatttg caagtgaaga ctgtagacga ggggcactac tctatatatg
     7921 atcaaactat ttgtttctac gaatcattag gattttcaag gttggaagtt tttccaaatt
     7981 tatgggatga atggaaccca tgtttagttc ttgtcaaaaa attggaacaa aagtgagcaa
     8041 tagatttaat actaatctgt gcttgatgaa aattctcact gtaaaaaatg ctgaaaatca
     8101 agtataatac cataggaaca aactggaagg tgaaaaagag catggcacaa ctatttttta
     8161 agtatggcgc aatgaatagc ggcaagacga tcgagatcct aaaagtcgca cataattatg
     8221 aagaacaaaa taagccagtt gtcttaatga caagcggtat tgatacaaga gatggtttag
     8281 gcgttgtttc aagccgaatc ggactgaaac gtaatgcgat gccgatattt gaagaaacag
     8341 acgtatttga cgtaattcag aaaatggaaa gcaaacccta ttgtgtgctg atcgatgaag
     8401 cgcaattctt aaaaaagaaa catgtcattg cttttacgag aattgtggac gaattggata
     8461 ttccggttat ggcatttggt ttgaaaaatg attttcgaaa cgagttattt gaaggatcaa
     8521 aatatttatt gctttatgca gacaaaatcg aagaaatgaa aacaatctgt tggttctgcc
     8581 acaaaaaagc aatcatgaat ttacattata ttgatggaaa ccctgtttat gaaggcgatc
     8641 aagtgcaaat tggcggaaat gaagcttatt accccgtttg tcgaaaacat tactttcacc
     8701 catcaatgat tacagaagga gaataagcat gtacgatcaa ttacaagcaa ttgaagaccg
     8761 ttatgaagag cttggagaac ttttaagtga cccagaagtg atttctgata caaagcgttt
     8821 tatgcagctt tcaaaagaag aagcaaacac gcgcgaaaca gtcgaagttt atcgtcgtta
     8881 taaagaagta gtacaaggaa tcaccgatac agaagaactg cttggtgaaa aactggatga
     8941 tgaaatggct gaactagcaa aagaagaatt atctgaatta aaaaaagaaa aagaagtctt
     9001 agaagaaaaa atcaagatct tgcttttacc aaaagatcca aatgacgata aaaatatcat
     9061 catggaaatc cgtggagcag caggcggaga cgaagcagca ttattcgctg gcgatctttt
     9121 caatatgtac caaaagtatg ctgaagcaca aggctggaaa acagaagtga tggaagctaa
     9181 cgttaccgga atcggtggat ataaagaagt gatcatgatg atcaccggag acaacgtata
     9241 ttcgaaatta aaatatgaaa gtggtgcgca ccgcgtccag cgtgttcctt caactgaatc
     9301 gcaagggcgt atccatactt ccaccgcaac agttgtcgtg atgccagaag ctgaagaagt
     9361 agagatcgat attgaagaca aagatatccg tacagatatc tatcatgctt caggtgctgg
     9421 cggacagcac gtcaataaaa cagcatctgc tgtacgtttg actcacttac caacagggat
     9481 cgttgttgct atgcaagatg aacgttctca aatcaagaac cgtgaaaaag caatgaaaat
     9541 cttgcgtgct cgtgtgtacg ataagatcca gcaagaagca caaagcgagt atgatgccaa
     9601 tcgaaaatca gcagtcggaa ctggggatcg ttcagaacgg atccggacgt ataatttccc
     9661 gcaaaatcgt gtgactgatc atcgtatcgg attaactatc caaaaattag atcagatttt
     9721 agcagggaaa ttagatgaga tcgttgatgc attgatcatg tatgatcaaa catcaaaact
     9781 ggaagctatg caaaatgggt aaaatgacct atcgagaagt cctgtcacgg gcttcttctt
     9841 ttttagagga tcatgggaaa gaagcatata gtatccaatt tttatttttg gaacgaaaac
     9901 aatggaaaaa acttgattgg ctccttcata tgaacgaaga gatttcagaa gaagaacagc
     9961 ggttgatcga aaccgatctg cgacttctgc ttgcagatca tccacctcag tatttgctag
    10021 gatatgcaga tttttatgat catcggttaa aggtaactga agcaacactg atccctcgac
    10081 cagaaacaga ggagttagtc gaatggtgtt tagacgagac gcctggtgta ccgttagaag
    10141 tgatagatat tgggacaggg actggagcaa ttgctattag tttgaaagca gcaagaaaaa
    10201 attggcatat atctgctgtg gatctttcag aagaagcatt agaagtagcg aaagaaaatg
    10261 ctcagaaaga aggaacgaaa atcagttttt atcatggaga tacattagga ccagtaatgg
    10321 atcagcaatt tgatgtgatt atttctaatc cgccgtacat cagtcggaat gaatgggaac
    10381 tgatggatga aagtgtccgg tcattcgaac ctaaaatggc cctttttgct gagaacgatg
    10441 gattagctat ttatgaaaaa atagcagttg aagcaccaag cgtactaaaa tcaaatggaa
    10501 agattttttt agaaatcgga tttcgccaag gtgaagcagt aaaaaaaata tttcagcaag
    10561 catttccaga taaaaaagtg gcgataaaaa aggatctttt tggcaatgaa cgaatgatcc
    10621 aagtttatgg ttgaagttgt ggatgaaaaa ttttttattt gtttttttat tataaaaaaa
    10681 cacttatttg acaaggatgt aaacgacttt ttggtttatc aacaagttta tccactattg
    10741 acaaatagat tatccacaaa cagtggaaaa cttgaggata aactcagaca aaataagaat
    10801 gtatgaaaaa ggggttgtgg ataaactgtg gaaacgaaac gatatgatga aacagaattg
    10861 caagaggcag ccgaagcact gaaagaagga gaacttgttg cttttcctac cgaaacagta
    10921 tacggattgg gcgccaacgc tcttttacca aacgctgtca agaaagtgtt ttctgtaaaa
    10981 gggagaccgc aagataatcc attaatcgtc catgtcgcct cttttgaaca agtaaaagag
    11041 tatgtggaca actttcatcc ggcagcagag aagattgtga agaacttctg gcctggacca
    11101 ttaactttga tatttaagat caaaaaagac actttgccct cagttgtgac gggaggatta
    11161 tccacagctg catttcgtat accggataac aaaaaaacac tagaagtaat cgaactttcc
    11221 ggtgttccgc ttgtcggtcc aagtgcaaat acgtcgggaa aaccaagtcc aacgactgca
    11281 gatcatgtat atcatgatct acaaggaaaa attaccggga ttattgatga tggagctacg
    11341 cgaattggtg tagaatccac tgttcttgat ttaagtgatc caacagccat gcctatgatt
    11401 ttacgaccag gtgcagtaac gaaagaacaa atcgaagcag tcatcgaatc tcctgttgct
    11461 attgatcagc atcttgtaaa agaaaatgaa acaccaaaag cccctgggat gaaatacaaa
    11521 cactactcac ccgatacacg tgttctgatg gtcagagatg gcgattggtc aactgcagtt
    11581 caatgggcaa aaaataagaa gatccgtgca ggtgtcatag caagccccga aattgcggat
    11641 caagtgcgga cagatactgc agctgtttat atgtataacg acaactcagt agaagcggcg
    11701 gctaaaggat tgtttgcagg tctaagagga ttagatgaac caacacttgg attagatttg
    11761 atttttgtac aagtgtatcc agaaactgga ttggggaatg cttacatgaa tcgcttgaaa
    11821 aaagcagcag gacaaaatta ttttgaaaaa tgatcaattg gttattgtcg cattctttta
    11881 gtatgataca atgctatcaa gtaaatcttc tatcagtgaa cgaaaaacta agcgtaacta
    11941 atcgtaatag aagagatacg aatatttggc aatttgtttt tgatgggaaa aataaaattg
    12001 taggaaaata aaaaggagct gttgtttgtg gtagattaca aaacgtttga ccctgatctt
    12061 tgggctgcga tcgccaaaga agaagaaaga caagaacata atttggagct gattgcctct
    12121 gaaaactttg tctccgaagc agtgatggct gcacaaggaa gtatcttgac aaataaatac
    12181 gcagaaggtt atccagggca tcgctattat ggtgggtgtg aatttgtcga tatcgttgaa
    12241 aatttagcga ttgaccgtgc aaaagaatta ttcggagcga aatttgcgaa cgtccagcct
    12301 cactccggct ctcaagccaa cacggctgct tatctcgctt tggtagaacc aggggacacg
    12361 atcttaggaa tggatctttc cgcaggcgga catctaacac atggatcacc agtcaacttc
    12421 agtggcaaaa cctatcactt tgttgcttac ggtgttgatc cgacaacaga agttattgat
    12481 tataatgtgg tacgtatttt agctagaaaa catcaaccaa aattgatcgt agcaggtgcc
    12541 agtgcttacg gacgaacgat cgactttgcg aaattcagag aaatcgcaga tgaagtaggc
    12601 gcaaaactga tggtagatat ggcacatatc gcaggattag tagcagcagg acttcatcct
    12661 aatccagttc cttatgcaga tatcacaacg actaccactc ataaaacatt gcgtggccct
    12721 cgaggcggca tgattttgac aaacgatgaa gcattagcaa aaaaaataaa tagtgcggta
    12781 tttcctggta ttcaaggcgg ccctctagag catgtgattg cgggtaaagc agtagcattc
    12841 aaagaagcat tagatcctgc atttaaagaa tatagtgaac aaatcatcgc caatgcaaaa
    12901 gcaatggtca aagttttcaa tcaagcaatt ggaaccagag tcatttcagg ggcgacagat
    12961 aatcatctga tgttgatcga tgttcgcgag ttaggtataa atggaaaaga agcagagagc
    13021 attttggaca gtgtgaatat cactgtaaat aaaaactcga ttccttttga aacactaagt
    13081 ccattcaaaa caagcggtat tcgaatcggc acgccagcaa tcactactcg cgggttcaaa
    13141 gaagaagatg ctgtaaaagt ggctgaacta gttgtcaaag cattacaagc aaaagatgat
    13201 aacgctcaac tagacgaagt caaaacaggt gtacgcgaat taactgaaaa atttccgttg
    13261 cataaaaaat aaaaatttaa attccattag taaacagaaa agttcaatgt tgggcttttc
    13321 tgtttatttt ttgggaaaac ggattctttg acaatatcgg tgatgctctc tagtatatga
    13381 ggtgtaattt tagtcttcac actaaatctc agggaggaaa agtcatgact gatcgtatct
    13441 cgttagaaaa ggaagcagta gcattcagtg atgcaaatgc gcctcatccg cgtatctatg
    13501 aactagcacc ggaagaagga agagttttac tagaagaagt acaagaatca cctgtgaaca
    13561 aatattccgt cgatattgaa gacttgatgg tggatactgg cgaatgggga aaactcaata
    13621 ttcgtctcgt acgaccagaa gggaatatgg ataaattacc agttattttt tatattcatg
    13681 gagcaggctg ggtttttggc agtgctcaaa cccatgataa attgatcaga gaacttgcgg
    13741 ttcgtacgaa ttccgtcgtt gtctttccag aatatagtcg atcaccagaa gcaaaatatc
    13801 caacagcaat tgaacaaaat tatgctgtgt tgcaacagct gaataaatta gccaagtcaa
    13861 aaaatctgga tctcgagaat ctaatcgttg caggagattc agtaggtggt aacatggcta
    13921 cagtcatgac gatcttaaca aaagaacgaa agggcctccc aatatccaag caattgctgt
    13981 attatccagt cacaaacgct gattttgata cagattccta tcatgaattc ggagaaaact
    14041 attttttgac aagagaagga atgatgtggt tttgggacca atatacgacg aatgaagaag
    14101 agcgtgctga aatcacagct tcgcctttga gagcaaatat aaatgaacta aatgatcttc
    14161 cttcagcaat gattttaacg ggagaagcgg atgtattaag agatgaaggt gaggcttatg
    14221 cgcgcaaact tcgtgatgca ggggttcctg ttacacaagt acgcttccaa ggaatcatcc
    14281 atgattttgt catggtaaat gctatggatc agacaaaggc aacgcgtgca gcaatggcat
    14341 tatctacttc ttggatcaag aaaaaagaat aagctttgct tgtccgcagg aaaacttctt
    14401 ttcaaaaacg gattttctta ggaaaaatgg cgagtttgac tagtcaatca cttgattttg
    14461 ttttacaatt aaaaagaaac gacagaacaa aggagagctt aaaaaatggg caaatttcaa
    14521 gtgatcgatc atccattgat tcaacacaaa ctaactatta ttcgggacaa aaactgcgga
    14581 acaaaggttt ttcgtgaagt agtcgatgaa atcgctatgt tgatggcata tgaagtatca
    14641 cgggatatgc cattggaaga catcgtgatc gaaacaccga tcactgaaac aacacaaaaa
    14701 acgttgtctg gtaaaaaagt agcgattatt ccaatcttgc gcgcaggtat tggaatggtt
    14761 gacggtatct tggaattgat tccagctgct aaagtgggac atatcggtct ttatcgagat
    14821 gaagaaacat tggaaccaca tgaatatttc gtcaaacttc cagaagacat tgatgcacgt
    14881 cagttgtttg tagtagatcc aatgttggca acaggcggat cagcaatcat ggcaatcgat
    14941 gcattgaaag aacgtggcgc gtcgaacatt aaatttgttt gtttggtagc agcacctgag
    15001 ggagtaaaag ctttgcaaga agcgcatcct gatattgaca tctatacagc ttctctagat
    15061 gaaaaattga atgaacatgg ttatatcgtt cctggactag gggatgcggg tgaccgtttg
    15121 tttggtacaa aataagtaaa tacgaaaaag ggtgtgacac aacttttgtc acatcctttt
    15181 ttggtcattt ttaacgtttg atgcagaaca gctttttcac agctttcgtt tgcatataaa
    15241 aaaaccatcc caaaaatgca gagatggtca aattcaaatc ttttttagtt ctttgtagtt
    15301 aacgattact acacccttac tttgcgtcat caagttcgca ttttctgcag tttcatcaat
    15361 ctcaacaaca gcggaatttt tcaattgctt tgaaatcgtt ccagtttgcg acttgccgtg
    15421 aattgtgaac attacgtgag tattgatctt gaatttttca ccaatttccg atggtacgat
    15481 agttgtatca aattttccaa aagttgccat tttcataaaa cctgcctttc acatcaatta
    15541 taccacgttt tatgaaaaat atcatgaaat taaactaatt ttccatcgga ctctttacaa
    15601 tcagccaaat agagtatata atttcttttg gataaggcca gcttagctca gttggtagag
    15661 caacgcactc gtaacgcgta ggtcgtaggt tcgatcccta tagctggcat taaaagagaa
    15721 gacagatata tcaaggattt ttaatccatt gatacatctg tctttttgtt taaaggaaat
    15781 aaagtatgct ttcttagcaa tagagcatgt attatttcta taaaaattta ggagaataaa
    15841 acgactagca atatagctgt gaaatcaaat atcacatgaa ctgcccaact tgtaatgatc
    15901 gagttgcttt taatggcagc ttggttgaat aacaatcttg ccaccccttg tatcaaaagg
    15961 atgtgtgcta cagtgtgtaa aatatttcca ttatcataag tactgtaatg gataaatcca
    16021 aagatgattg ctgacaagaa actagcgaca ggaatgctga acttactaga aaaaatagcg
    16081 agaaaataca ctgagaacaa ttcttctcca agtatcatga taggcaaaat aataaataat
    16141 aacgggctga aagcctcatt tattggattg cctgttaaac cccatttcaa aatttgttgc
    16201 aaaaataccg agactgcggc actgattact acattgataa aaaaatattt ggctatattt
    16261 ttgacaggag cgacaggttt cgagaacatt tttttcgtat cttttttacc aaaaacaagt
    16321 gtaaatgcta taaatccagt gatagttaaa gaaaaaatgc taataatgga tggcgcaaat
    16381 agcaacccta acaaaccgag tacaacgaca atagtgccca atatcccttt tttaagttct
    16441 attttttttc ctaaaatgtt attcatgata ataactccta ctattcagtt tttgaagata
    16501 gagtcagtat acatgaagag ttaaaatagg caatgttttt ttatggatat ttgtattgct
    16561 aaaaatttat cttatatatt cattttgatt atactacttt tatttgtaaa aattttctag
    16621 caaaaccaag tgcttgcaac tgattaagcg tacaattttt tagatgttcc gagcgtactt
    16681 caatcatttc aaaggagttg ctacttaaat cgatattttt taaatcagta tcaaaaaagc
    16741 tagcattttc taaatcatta gtagaaaaca ttattttagt gagggaacag tctacaaaat
    16801 ctgcgcttga taagacagag tgctcaaagg tactttcttt tatgtttgaa ccagataaat
    16861 ttacatagtc cattttacaa ttctcgaata acgtgttccc aatataactt tccatcaatt
    16921 tagtacctgt taatttacaa ttgacaaatt tcacgcgatg caacatgctt tttgtcaata
    16981 tacagcctga aaaatcacag ttttcgaata tacagtcggt aatctccatt ctttgaaaat
    17041 cattggaagc aaaaaatgca tacttgaaaa tagatttgta aataattgga tggcagatta
    17101 tttggttttc gatgacctct ttttgtgaaa ggatattata cagttcaaaa tcttcgtttt
    17161 catagatatg aaaaaaggat cctttattca agcttgcttc attaacttta ggaaaaatca
    17221 tatttaatta cctccatttt cataaagtca tattaacaaa agctaaacca acttacatta
    17281 gcagaaaatg agataggttt tcgccttttt cgataacgat agtgtcgcta tctcttgaaa
    17341 cgttcttaga attgccctga gagacacaaa ttaggcgtaa ttctaataaa aatcttagaa
    17401 taaaaatttt tactgattta atttagaatg tttttaaaat aaaaatcgtt gccataacag
    17461 tgtattgaaa ggttttaaca atccagtaat gatttaaaaa aatctagatg tagcgtatac
    17521 tttgtttata gaatgtagtg ggaagaaata tatttttggg aggaaatcgt gatgtctaaa
    17581 agtgaagaaa tagaaaacat ggtactaaaa ttatatgatc tcgttttaaa tccagagatc
    17641 aaagaaaagg aacgtatact tttgatagac gcaaagacag gcttagaaaa aggtcagtat
    17701 tatcctaaag tcataaataa tttggaacgt agtttacgtc cgttagcaat caggggagaa
    17761 ttgtctaaac cagtatcacc gttttatatg gagatatcaa caataggaaa atttgaaaaa
    17821 gaattaggtc gaggaatggc ttcggcaccg attacttttg ggcatttata aaaacgagtt
    17881 attcatctca atgaattaaa aagtatagtc aatctcataa tgcaggctag gttcatttat
    17941 ttgaacagag tctgtatttt tttattctaa ataaagaaac agatgtttct ttatattgta
    18001 ttttatcctt taattaacac aaaaacagct agtataaact aataatgtta attaaaagtg
    18061 tattttatga aactatcgtt atttattaat gttgcatttt tgtaagttaa aaattggaaa
    18121 tatag
//
LOCUS       DAZABD010000052        17913 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000052 DAZABD010000000
VERSION     DAZABD010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17913
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.052"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(363..1913)
                     /locus_tag="ACHALG_001976"
     CDS             complement(363..1913)
                     /locus_tag="ACHALG_001976"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFN2587352.1"
                     /translation="MSDKNTYACRSQRHKQESDTYENPDQLNETVTSKTANENKQSRI
                     PKKNSNYSKYIFALFTALILVTVATAGYIVYKLKQQEAEAKAKYETAAKNLMASIQEE
                     QDQSRISTKIDTISDGENKCLVYRPVDESAVPFKNANQLLDELAQKQQKKHCEKEVLT
                     VARIKATAISSKVGQYSIEADSFIWDRSKEHFKNPDNISEKAIYVSEKTGKEITNKDL
                     IPDEGSLLGIQQVIQQSILDHAEKPEKIIDAVLTMDRISYDSKMTYTPDELTIILPNN
                     SIGTSKVTLNYKDIAPFIDTDLVSQESIKDALPALDENKKYVALTFDDGPNNSSTLDL
                     LNILKTDNVKATFFMLGQMVDQNPDVAKQAHDEGHEVACHSYSHPQLNALSTDELRSE
                     INEANKAIFKATGVLPRNIRPPYGAIDKKSAETIGMPIIQWDIDSQDWKSKNPRTINN
                     VVKQNVYSGAIILIHDIHPESVKAIPNMISMLKNEGYEFVTIDQLLSGKQKTLHQYFG
                     MNDERLVD"
     gene            complement(2396..2635)
                     /locus_tag="ACHALG_001977"
     CDS             complement(2396..2635)
                     /locus_tag="ACHALG_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587353.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSV
                     DTVHRALKKDPDCCFIKVNTGRSRRLAKRYNILIN"
     gene            complement(2659..4182)
                     /locus_tag="ACHALG_001978"
     CDS             complement(2659..4182)
                     /locus_tag="ACHALG_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFN2587354.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLIQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKLNKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNEMHNWPTFYPASLSEHEIAQEMMAH"
     gene            complement(4200..4322)
                     /locus_tag="ACHALG_001979"
     CDS             complement(4200..4322)
                     /locus_tag="ACHALG_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFN2587355.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            complement(4352..5335)
                     /locus_tag="ACHALG_001980"
     CDS             complement(4352..5335)
                     /locus_tag="ACHALG_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFN2587356.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHREERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKHYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQGVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(5359..5508)
                     /gene="rpmG"
                     /locus_tag="ACHALG_001981"
     CDS             complement(5359..5508)
                     /gene="rpmG"
                     /locus_tag="ACHALG_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFN2587357.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(5529..5906)
                     /locus_tag="ACHALG_001982"
     CDS             complement(5529..5906)
                     /locus_tag="ACHALG_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587358.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(5938..6117)
                     /gene="rpmF"
                     /locus_tag="ACHALG_001983"
     CDS             complement(5938..6117)
                     /gene="rpmF"
                     /locus_tag="ACHALG_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFN2587359.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(6132..6401)
                     /gene="rpsN"
                     /locus_tag="ACHALG_001984"
     CDS             complement(6132..6401)
                     /gene="rpsN"
                     /locus_tag="ACHALG_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFN2587360.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            complement(6923..8689)
                     /locus_tag="ACHALG_001985"
     CDS             complement(6923..8689)
                     /locus_tag="ACHALG_001985"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN2587361.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAACL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKVVDQGTHSELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(8649..10403)
                     /locus_tag="ACHALG_001986"
     CDS             complement(8649..10403)
                     /locus_tag="ACHALG_001986"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN2587362.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(10513..11082)
                     /locus_tag="ACHALG_001987"
     CDS             complement(10513..11082)
                     /locus_tag="ACHALG_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HFN2587363.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(11100..12515)
                     /locus_tag="ACHALG_001988"
     CDS             complement(11100..12515)
                     /locus_tag="ACHALG_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN2587364.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNL
                     FLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGGIKK
                     DIKLRVEDSTLIEQIFNDITK"
     gene            complement(12526..13194)
                     /locus_tag="ACHALG_001989"
     CDS             complement(12526..13194)
                     /locus_tag="ACHALG_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFN2587365.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(13324..13908)
                     /locus_tag="ACHALG_001990"
     CDS             complement(13324..13908)
                     /locus_tag="ACHALG_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN2587366.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(14239..14472)
                     /locus_tag="ACHALG_001991"
     CDS             complement(14239..14472)
                     /locus_tag="ACHALG_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFN2587367.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(14487..15437)
                     /locus_tag="ACHALG_001992"
     CDS             complement(14487..15437)
                     /locus_tag="ACHALG_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein"
                     /protein_id="HFN2587368.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(15434..16276)
                     /locus_tag="ACHALG_001993"
     CDS             complement(15434..16276)
                     /locus_tag="ACHALG_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease subunit"
                     /protein_id="HFN2587369.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            16598..17785
                     /locus_tag="ACHALG_001994"
     CDS             16598..17785
                     /locus_tag="ACHALG_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFN2587370.1"
                     /translation="MYFLGIDIGKRTHVASIMNEEGKVLLKGFSFPNTTEGAESLIER
                     MVDYSGAPSDFVIGMEATGHYWLSIFSYLHESDYLIHVVNPLQTDGWRKGTEIRKRKN
                     DIIDSVLIADLMRYGSFVETVLSDENVFSLKQLSRYRTYLVGTASDFKRKIIAVLDQV
                     FPEYATIFTKQGVFGKASKELLLAFSAPIDLENISSETLAQYLAELSRNRIKAEKADQ
                     LKQAASNSFGVKFAQEAFTFQLRSMIEQLKFIESQIKETEREIKHIMDTLNSVILTIP
                     GIGPINGATILGEIGDIQKFSNPKKLVAYAGIDASVTQSGQYEATHNVMSKRGSPYLR
                     KALFSAALVASQCDPVFSAFYQKKVSEGKHHLTALGAVSRKLCYVIHAILTKNEPYII
                     VSD"
BASE COUNT         5645 a         3311 c         2548 g         6409 t
ORIGIN      
        1 cttttgactt actattagta ttaataacta gtttaattat gttaaaatta aatcaactca
       61 ataaatcttc atcgcattaa ttgcattctg taaactaata aagttatatc tagcttgtat
      121 aaactttttc aaaaaggttt caataatgag tggcattata aaaagaaatg ttttttcaac
      181 caattttgtg atgtgatttt tgatcacaaa acttccagta ctttttttat ttactaaaat
      241 agaactttga ctaataagaa ttagcattct cgatactctt tatcaatttg ttttgcttaa
      301 cattttttct gagagagaat tgcaagtata acttctacac ataccaagca atctaataac
      361 agttagtcta ctagccgctc atcattcata ccaaaatatt gatgcaatgt tttttgtttt
      421 cctgacaata gttgatcgat cgtcacaaat tcgtatcctt cgttttttaa catagagatc
      481 atatttggta tcgctttgac tgattcagga tggatgtcgt ggatcaagat gattgcacca
      541 ctataaacat tctgtttgac aacattattg attgttctag gattttttga tttccaatct
      601 tgtgagtcaa tatcccattg aataattggc ataccaattg tttcagcact ttttttatca
      661 atagcgccat aagggggacg gatattgcga ggcaagacac ctgttgcctt gaaaattgct
      721 ttattagctt catttatttc acttcgtaat tcgtctgttg aaagtgcatt caattgtgga
      781 tgagaatacg aatgacatgc tacctcatga ccttcgtcgt gagcttgttt cgcaacatct
      841 ggattttgat ccaccatctg tcctaacatg aaaaaagtag ccttcacgtt gtctgttttt
      901 agtatattca acaaatcaag tgttgagcta ttatttggac catcatcaaa agtgagagcg
      961 acgtactttt tattttcatc taacgcaggt aatgcatctt ttatactttc ttgcgaaacc
     1021 agatctgtat caatgaatgg cgcaatatct ttataattta gagtgacttt agaagttcca
     1081 attgaattgt ttggtaagat gatagtcaat tcatcaggtg tataagtcat tttactatca
     1141 taagaaattc tatccattgt taatacagca tcaataattt tttcaggttt ttcggcatga
     1201 tcaagtatgg attgctgaat cacctgttga atacctaaga gacttccttc atctggaatc
     1261 aaatccttat ttgtaatttc tttgccagtt ttttcggaaa cataaatggc tttctcagaa
     1321 atattgtctg gatttttaaa atgttcctta ctacgatccc agataaaact atctgcttca
     1381 atgctatact gaccaacttt agaagagatt gcggttgctt ttattctggc aacagtaagg
     1441 acttcttttt cacaatgttt cttttgttgc ttttgagcaa gctcatctaa gagttgattt
     1501 gcatttttaa aaggaacagc actttcatca actggacgat aaacaaggca cttattttct
     1561 ccgtcactta tagtatctat tttcgtcgaa attctacttt ggtcttgctc ttcttggatt
     1621 gaagccatga gattttttgc agcagtttca tattttgctt tagcttctgc ttcttgctgt
     1681 tttaatttat agacaatata gccagcagta gcaacagtta ctagaataag tgcggtaaac
     1741 aatgcgaata tatacttgct ataattcgaa tttttcttcg gtattcttga ttgtttgttt
     1801 tcatttgcgg ttttggacgt tacagtttca ttcaattgat ctggattttc ataggtatca
     1861 gattcttgtt tgtgacgttg gctgcgacat gcataggtat ttttgtcaga catatggcct
     1921 tattcctcca tttatcatca tataaataaa acatagaaat ttcttatttt ttacctggca
     1981 tttttcacta tttttttact tttgccgtag taattataga tgttttctca gcattttata
     2041 cgtacaccaa tttaaaaggt ttttttacat atttcttctt cctctcttat tttttagctg
     2101 aatgtctaaa aaaataatag catcattttc ggtatcagaa aataagttat cataaataat
     2161 atccttacat ttttgtcatg tttttaaata tgttagttga atcccattct aatattacct
     2221 attcttcatt tttactgcta attatctctt ctatatgatt taaaagtgtt gtttttctta
     2281 aatcaaaata actagtagaa ttgtgacata tttttcatag gaaacctctt tctatgcata
     2341 ataaaaagca gatcaaattc cattaaggaa gaattgaact gctttagtgt tacaattagt
     2401 ttatcaagat attatatcgt tttgctaaac gtctacttct tccagtatta accttgataa
     2461 aacaacaatc tggatctttt tttaatgctc tatgaactgt gtcaacacta acatgtaaag
     2521 ttgcagcaat atcagctaat gtactcccat ttaatctcat ttgctttgca aatcgataac
     2581 gccgataagc atcaatattc ttggaatcca tagacaataa tacttttctt accatgtatt
     2641 tcctccaaag attggatttt aatgggccat catttcttga gcaatctcat gctcagataa
     2701 tgaagctgga tagaaagtag gccaattatg catttcatta taaagttttt cttgactttc
     2761 tccaccaaag aaaatatgga aatgctctgc cttacttggt gcaattccat gatcgctaaa
     2821 ttgaatatat ttaaaacgac cagcacctgc atcatcagtt tcaaaattaa aacgaacacc
     2881 acggtttcct ttttcataat tcaaaatttt gtatccaaca tatttgtatg tgtattgatg
     2941 atgttcaccg ttgactacaa aatcaatggt gttgtcttta atgttgatag tatcaatatc
     3001 tgtcttatag ccagtagtat aataatcttt gtactcggca gctgtcatgt ctttattgag
     3061 ttttgctttg taatcgaaaa cttcatccaa aattccatct tttaacaacg gataaacaga
     3121 ttgccattcg cctgtataat cactcaattc gcgatccttc acatcggcat ctttaaagta
     3181 cccattagca actgtctttt catccttcgc ttctttttct ggaactacct cttcaccagc
     3241 aaccatagtt gttttctcta acgcttttag attgtcttcc attactgaaa cataatcttc
     3301 accattatcc atttgttctt ttgtaagact ttcaagagga ttcaatactt ccaattttat
     3361 cccagcttca ttggccaaag tttttgcgat tttatcatta gcattctttt caaagtaaat
     3421 ataattgatc ttattttttt taacatactc ttttaattca gctaatcgac cagaagatgg
     3481 ctcttcttca ggacttagtc cagcaatagg aacttgtatc aatccataat ccaaagctaa
     3541 atatccaaag gctgcatgtt gtgtcacaaa acttttttgt ttcgcttctt taagactagt
     3601 ggtatagtca gcatccaacc gtttcagttt agtcaaatac ttttcagcat ttgtctcaaa
     3661 tacttttgct tttttcggat acaatttaac taactgatcc ttgatatttg aaacttcctt
     3721 aattgccatt ttgggtgaaa cccacgtatg aggatctagt tcatgatggt gatcttcttc
     3781 tccgtgatca tgatcttctt cttcactacc tggcaataaa atcatgtctt ttgttccttc
     3841 tactacatta ggcccagctt tcttccaact ttctttagct tttggtaccc atgattccat
     3901 gttttcgtta tgatatacaa aaacatcagc atcatgagct tttgccatat ccttagctga
     3961 gggctcatag tcatgaggtt ctgatcccgc tggaaccatt aaatccacgt tcgcttcatc
     4021 gccgacgatg tttttggtga aatcatacat tggatagaat gttgttacaa tttctaattt
     4081 tccatcttta tcattcgata cttttgcatc attagtgctg gagcaagctc ccaataatcc
     4141 tagagctgct atcccaataa gtcctacact gattttcttc attctttttc cttccttaat
     4201 tatgctaatt tcttcatttt atttcgttta tgttctttga taatttttaa tgcccttttt
     4261 ctagttttaa tattcgggct tttaagtcgt cgatatgcac tagatatatc cgttttctcc
     4321 attagaaatt acccccaatc aatacttatc attatttgct ttccgccagt aatttatcaa
     4381 aactctgttt gatcctttct tcatccaaca tttttccgat tagaataata gtggtttggt
     4441 ctttttgtcg agtggtgtat tgaaaattaa cctgttcgtt taccccttgc atggcaacta
     4501 taaaatcctg ctcttgtaaa gcaatcaatc ctttaaaacg atacaggctc ccttggttgg
     4561 ttaaaatcag ccagtcaatc cattggagta aaaaatcttt ttttatatct tgatctgaag
     4621 ttaagagaat tgatttaaac tcatgtactt gatggtgttg atgatagtgt ttgccattat
     4681 agtgattttc tgatgaatca ctttcaaaaa ctaagggatg attaaatttt ttcaaattat
     4741 aaaaatcctt tttttgaatc gtttcactat gcgtgaacat tcgaatatcg gataaagggt
     4801 taatactttt gatttctctc tgtaattttt ttagtgcaga taaattcaag ttttgttttc
     4861 tagacacaaa aatgcgatct gccatgacaa gttgcttaag ggcttgtggt tctctttcaa
     4921 taagttcaaa actgtcacta tcaataacag tcaataaact atcgatatat actcttcctc
     4981 ttagttgagg cgctgttaaa agtgtttgaa tgattggttg gggatctgct atacctgtag
     5041 tttcaatgat gatttgtgaa attggttgtt gatgttcttc tattgtttcc agtaaagaaa
     5101 tcaatattgc taataaatca ctgcgaagac tgcaacacat acatccatta tttagttgta
     5161 acacccgttc ctctctatga actaataatt tgtgatcaat cccaacttca ccaaactcat
     5221 tttcaattat aataatttct tccctggggt agggtgagac ttgcaaggct tgattgatta
     5281 gcgttgtttt tccggcccct aaaaacccag aaacgattgt aactggtatg cccattttgt
     5341 caccttttta ccacttagct atttcacttc tttgaaaatt gcccgtctgc gcaattttgg
     5401 cgaatacttt tttaattcta accgttcagg gttattccgt ttatttttgc tggtgagata
     5461 cagacgttct ccagtttcga cacattccaa aataatattt tgacgcataa atcttcctcc
     5521 ttcaatactt atcgatcttc aagatatgct tgaatttgtt gtgcatcttc aaaagttaac
     5581 tcgccagatt ccaattgtga aactaaattt tctttcatat caacaggtat ctcccaataa
     5641 ccatcaatac attgaaataa ctcctcgtca tactcttcaa aaattggata gattcttaaa
     5701 aaatcatcaa atgataagcc ttggtcctca tagtaatctt tgactaatcc attttcaatt
     5761 gcctgttggg tcggtaaacc cgtttcagta ataattcctc tctctaccat cgaacgataa
     5821 aaagtgcatt tatctagcga ttgctgggtc gtttcaagat aatactttaa tccttcttca
     5881 actaaaggtg ggtaaataat ttccattttg attccctcct aaaagttata tattaaacta
     5941 ttttgaagct cctttaatta ctttctttcc attatagtac ccttttaaag atacatgatg
     6001 gctacgtcta taatcaccat tcgcttcgtc aaatgaaatt tctggtttta ctacagtttg
     6061 atgagttctt cttaaccgtt tcttcgcctt agatgttttt cttgctggta ctgccatgat
     6121 atttcctcct tttaccaact tgcttttttt actccaggaa tcaacccttg atgggcaagt
     6181 tctctaaact taatacggga cataccgaac tttcgcatat accctcttgg acgaccatcc
     6241 gtctgatcac gaagcctcag acgattcgga tttgaatctc ttggcaattt agcgagagcg
     6301 gtgcgatctc ctgcggcttt taattcctga cgtttatctg cgtacttttc aatcatcgcc
     6361 atttgtttct ttgctttagc aattttcgac ttttttgcca tataccttta tactcctttt
     6421 attttgattt agacatctca aatcgtaatc gttacgtttt gacactgata ctttaccaac
     6481 aagagttgct cttgtcaaca ataaataaca tgaaataaaa ctcatatgaa tagataacta
     6541 tttgaaaacg aatttatcgg tacatagaga taatcaaatc atttaaaaga atatgcagtt
     6601 taagctctga aaaataagaa ttcactttta ttagtccaat taaacacaaa taccgtttta
     6661 agtagctttt cctctactta aaacggcaat atgaaagaaa ctataacttt ttattcaatc
     6721 ttttatgttt aactggttta tcccaatttt ttaaaacttt gattattcct tatattataa
     6781 gaatagacat taagtgaact aattcaatca tactgattct ttcgtgtatt caaaattaga
     6841 tgctgtttaa taaaattatt ctttttgaaa aagaattata actatatccc aaaaattgta
     6901 ggttaattta aagcctattt catcatcgtg attgagcttc tttttcatag acgaatttct
     6961 cagatagttt agttttcttt atgagatttt tgtatatttc tgaccttcga tacaactcac
     7021 tatgagtacc ttgatcaact acttttcctt gatccatcac tacaatttta tttacctttt
     7081 caatagactt caagcgatgc gatataatta gtactgtctt atcttgtatt aaacgattta
     7141 aactttcctg aatacgtttt tcattttccg catctaaact ggcagtaatt tcatctaaaa
     7201 ttaaaattgg cgcatttttc aaaaaagccc gtgctatgga taggcgttgt cgctctcctc
     7261 cagaaagtga acttccgttt tctccgacca atgtatgata accgtcaggt agtttttcaa
     7321 taaagtcttc gcagttagct aaacaagctg cttttttcac ttcctcatct gacgcttgac
     7381 tatttccaat acgtatattt tccaaaatac ttgtattaaa caaagttacc tcttgaaaaa
     7441 caatagcgat tttgctaaac aaagaagctg gagatacacg tttaatatca tacccatcaa
     7501 ttaaaataca gccttcatca taatcataca gtctagacac taatttcaag atactagttt
     7561 tcccggaacc actagcacct actaacgcag tcacttctcc ttgtttagcc gtaaaagaga
     7621 tatgatctaa aattggggta ttgttatcat atgagaaaga gacatctcgc aactcaatgt
     7681 caaaagattt cagaggacta tctgatccct cttggatact ggtttccttc atagctctaa
     7741 ttcgctgtat tttaggagct aaataaaata tttcaagcac tgcttctttc atagaatcga
     7801 aggaatcctt tatttttatt gcggctagta agtaaccaac gacgtagagt atagtcactt
     7861 cacccgttaa cagtaagtga acacctacta gaatgactac tgctaaacta acatatgaga
     7921 aaatagagga taaggccatg actgaaaaag tacttaattc tacctttaaa tgaatccttt
     7981 cactctcttc catttttttg aaaagtctgt cttgaacttt tttagataga ttaaagctat
     8041 taatctcttg ctgcaattct atagtttctt gaaattcttc cgagttttct ctcaaagtat
     8101 cgtaatattt agtattggct ttcgtttggg atttctttga taacaagatt aagacaaaac
     8161 taaataacgt tggcaaaata acagctaatc ccattttgac attaccaacc aaaagcatca
     8221 ctgaaataaa agggaaaaac agagccatac cgccggcttt aggtattgca tgactcatcg
     8281 catgctcaat accttcaacg tcagacatga tagtttgaga taaatctgat aaattatgtt
     8341 ttgaaaaata ggataatggt aaatttgaca aatcgtctgc aatttgcact cttaaatgcg
     8401 cactttcttt ataagtacta ttatataagt tttcatactc cctatctaac aaccaataca
     8461 tgaaaagcaa agtaacaaac gatacggcaa aataaagcca acggggcttt gtattttcta
     8521 aaactaattc gtctatcagc atcattataa tgaatgctgg taccatatta atgcagtata
     8581 caaaaaaact agaaatagaa gctttagtta aagcttctgc gccctgatca cttaaagcaa
     8641 atttgttttt ataaaaactt ttcattgtca agcctccaat catttgcatt ctcataatta
     8701 gtgaccaatt gtttatacac ttgatgttta gccattaatt cattatgatt tccacgttct
     8761 actattttcc cttctgacaa gacaagaatt tcatctacag atttaatgct ggataatcga
     8821 tgagctatca taataactgt tttttctttc atcagatgtt gaaaagcctt ttgtagttca
     8881 tactcattat ctggatcaat agatgcactg gcttcatcca taataacaat ctttgcatct
     8941 tttaaaattg ctctggcaat agctatacgt tgtttttctc ctcctgacag ataaactcct
     9001 ttactaccaa ttaaggtatg ctctctttcg ggaaattttt ctaaaatcga ttcgcaaccg
     9061 gctaatctta gcgcgtcaaa tactttactt gctggcgctt gaggatttgc tatagaaacg
     9121 ttatcgtaaa tactctgatt gaatagtttc gtatcttgaa agacaaaagc aattgtgtta
     9181 ataattgtgt cttttgaata gtgatttaaa ggcttaccac caattttaat ctgtccctta
     9241 tccacattgt agaaacctga aattagttta gctatcgtac tctttccgct tcctgatttt
     9301 cctactaatg cataaattct tttttgttct aaaactaagt tgaaattttc taaaacacac
     9361 tcttctttgt atgaaaagct gacattttca aatgcaatgt cacaattttt aatctgtgtc
     9421 tcgttcccat attttagttg gtcttgcttc atttctaaat aaagggagtc tagtgtttcg
     9481 actgcatatc ttgcttcaaa caagtgcata cttacataca ttattctcat gaaagaaaca
     9541 aaaataattc cacttaaaaa aaacaacatc aacaagtcaa cagctaatat cttgggagtg
     9601 gtaagagaag ttataaaaaa ggttatagga actatcaaaa tgcaaactat gccaaaaaat
     9661 agccattgaa aaagaacaaa aggtgttttc ccactcaaag aatacttata tgcaaactta
     9721 gcataatcgt taattgcttt attcaaggct ttaaatgatg ttactttagc accaaatatc
     9781 tttataactt gaattccgcg gatatattct accgtttcag agcttaacac atctaacgat
     9841 tcttgatatt tttgaataaa atttgtattc tctgtcattt tctttaacaa gaatacacca
     9901 attactaaca tggcgattag aaccaatcca attttaaagt taatgaaaaa gcctaaggca
     9961 actgataaaa caggtgttac tatggcttga gcactatctg gaatcaagtg agcaattgcc
    10021 atatgtgttt tagccgcgtt gtcatcaatt gtctttcgaa tatatcccga agaatttaaa
    10081 tcaaaaaaac gaaaacttgc ttctgttaaa ccgctaattc ccttttttct caaattagtc
    10141 tctaagcgaa acccaaggat atgagaaaat aagccagaaa aaatataaaa taaagctcct
    10201 aaagttaacc aagcaacagt ttgaaccgca taactcttag ctaaatattc atctcctaca
    10261 ataattagag actgaagaaa tttgtaaata ctgtaatatc catagactgt taatacgaca
    10321 gaaaaatcag aaaataaagt ggctaaccat ccgtagtatt tcattttagg gacataacta
    10381 aataattttt tgtatacttt cattttttcc tcccccgcat tcaatttctt ctcttattcg
    10441 tttacaactg ttaatgcttt ccgtaatagc gtaaattaga gatgtacaaa cagataaaag
    10501 gtaaactacg ttctattttt tgtatttttt taagactttt cttccaatca atacaccaat
    10561 catagataaa cttgctacaa ctaacactga aatgattccc catataccta ccgcatagtg
    10621 gatcattgca tccattgttg cagcagattg tcctttatct atccaatctt gacgaaaacg
    10681 atctgtaaag aaccatatag gaatccactg tccggcagca ctgcctaatt gcattagagc
    10741 ccaagacgca acttgaggaa ttgctcgatc atatccaaat acagatagaa taatatcagc
    10801 taagatcgcc atactaatcg aactgataaa ccaaggtaag tacgccccgc ccattaacgt
    10861 aaaaaataac aataaaacaa ttgtgtaaat agtaaaaatt ccttttctag ggtagttata
    10921 gctaatataa acaaatatga ttccagataa taatccaaaa atcccggggg atatgctgtg
    10981 accaaacgcc ccaccaatac tactaaccat tattgaaaaa aagtaaatta ctagggaaat
    11041 aactgtagct aaaccaataa aaatataatc ttttgttttc atatatattc tcctcaattc
    11101 tatttcgtta tgtcattaaa aatctgttct atcaaagttg aatcttctac tctaagtttt
    11161 atatcttttt ttattccacc attttttaaa taaataattc tattgcatgt tttataaaga
    11221 aattccggat catgagtagc tataactact gcaccctttt cttttaagtc tttaataagg
    11281 tcagcaatcc tttccatatt aatataatcc aacccacttg taggttcatc aaatagaaat
    11341 aaattagttt ttgacaatga agctatagaa accaataatc tttgtttttc tccaccggat
    11401 aaatcaaacg gtaattgatc tttcatttta taaagttcca ttttttttaa aattttttct
    11461 tgctgatctt tactaactat ttcattcccc agggctaact cgctcctcac tgattctgtg
    11521 aaaaattggt aatccatttc ttgcattact aaaaacggcg atgacaaatt ttttttattt
    11581 tcgattatct ttcctttggt agggcgaata atcttcatga gtattcttag taaggttgtt
    11641 ttccccacac cgtttttccc taccaaacca ataacatctc caactttgac atcaaaattt
    11701 atgtctttta gaatattttc ataagtaatt tcttcaactt ttaatacatc tgcacttacc
    11761 ttttcgatat agggaacttc aatcgaacta ttaaaaatgt caaatttcct taatgcgtta
    11821 ttgttttcag tttttaacaa ctgaatagtt gtagttttta cagttctatt tttaacaaat
    11881 agaacttgat caattaattc tttcaaatag taaaaacgat gttcagctat aacaatagta
    11941 aatcctttat tttttaaatc acaaagtaag tcagcaagtt tcttagtcgc tttgaagtcc
    12001 aaatttgaag acggttcatc caatataatt aatttagagt ttagcgataa actacatcca
    12061 atagaaattt tctgccgttc accacttgat agcgaaaaaa tgtttttatc taatatattt
    12121 gaaagatcta ataattcagc taattcattg attcttttat ccatatgctc ttttgtaaat
    12181 ccaaagtttt ccatcgaaaa tactaactca ctcgttgtat ttgttgtaaa gaattgtcct
    12241 ctaggatctt gacttacgta gccaatattt tttgatatac tttcttgtgt taaggattgt
    12301 agcgagttat tttcaaataa aatttcccca accaattttc cgtcataaag tcttggaata
    12361 agtccattca ttaactttaa tagtgtggat ttcccactcc cagaagaacc agtaatcaat
    12421 gtaagactgc ctttttttat tgaaaaatta aggtcaacaa gcgcttgttc cttaacatcc
    12481 ttatatgaaa aagaaacatt gttaaattca aacaattctt tcacctcaaa tcaaaatact
    12541 taagaaaaaa atagtaacgg aaaacaggat gatatagtct aataagctca tgtttgtttt
    12601 atagtaagaa cttcttttca caggagattc tatccccttt gttattgcat tagcggtcat
    12661 ttcttcagca atcatagttg cacgtataag aacgggaact aacagaaatt ccaaatattg
    12721 cagaggctga aaaaaagaca agcgaatgtt tctcattttt aaagattctt taatcattct
    12781 tatttcagaa cgataagtcg gaaagaatct gatcactact gtaatactta acattatttt
    12841 tttcggtaaa tgtatttttt ctaaagagga taataattca ttgctagtat agacactaac
    12901 caacaaccat gagatcatta cgaccggcaa cattcgaaat acaatatata aggatgtcgc
    12961 aaaaaatata gtaaaaaact ctatcttgat agcatcaata attttcatta ctaaaaacag
    13021 aactgaataa atcaagatga aataaataca tgtctttatt ttacgaaaca tcaatactaa
    13081 aactgtagca ataatgaaac accccacttg aaatatattt ccctttccta aaaaaataat
    13141 gaccggaaaa aaaatattta aagtaatcaa ggttaacgga ttgatttttg acactctttc
    13201 acctccaaaa aataagatcg cactgttatc ttaagctgat aaaaatgcac cgttattcag
    13261 ataacagtgc gatcttatca aaaagcataa ctgagaacaa ttctcaatgc aagctttttt
    13321 taattaactc agtaattcaa tccacccagg aacagaaaat tgatacaatc ttctggttaa
    13381 tagcatagtc ttttcttttg aatagctatg cttcaatata tcatagaatg tagcgtagaa
    13441 tgagtaaatg tagaagtgga tttcttcttc attaaagact aaattttgat tgatcattcc
    13501 gatagtcttt aacttatcta gacaacagac tgtctttttc gtcacaaatg atgtaatttt
    13561 ttcaacaata tcttgatatt tggttccagc tgattggaat aaaatcaaat cgaatatacg
    13621 tttattctca aaaatataat taataaatgg atctaattca ttgaagttct ctctccaact
    13681 tttctctata tctatatttt ctaagtttaa gaggtatttg gtaaactctt cattatacat
    13741 attaataagc gcattaatat gcggctgaat aatttcttca aataacgctt ccttagaagt
    13801 aaaatgttta taaaaagcac ccgtagttac gcctgcggtc aggcacagtt gccttatctt
    13861 agtttgctga aatccttctt tttcaaaatg gcttagtcca ctttctatta acttttggtg
    13921 agttataata taatctctgg tttctatggg tacttcctcc taataagatg aattaacttt
    13981 attatcttac acaggacttg aagtttcaat cgttaacgtt tattactgaa ctcattcctt
    14041 agagctaaac taatgagaaa gagcagaata actgatacaa aaagcaatcc tacaagaatt
    14101 aatagactca tctaaatcat tcaccttttc taaaataatc cgagaaagat ttaaaaaaat
    14161 tatatccagc tattaggata agtcctatca taatcactcc aatagccatt ttcttatcct
    14221 ccctaaaaat aaagtctttt acttaaaacc ttttttgtaa tcgtcaataa attttttccc
    14281 tttatcagtt aattgaaatc ctatatctaa atagtcctca tatctaagtt tagccagcat
    14341 ctcacttaca gatgctggac taacacttag aacttttgct aaatcattat tttttaattt
    14401 cttttttctt tttgataatt cctgaattgc tagtaaataa attagtatag tagtcgtttt
    14461 aatttgtacc atacctaccc tccaaattat ttcgaaaggc cttcagcaat tttatccagg
    14521 ttccatttca tcatcgcata gtaactatca ccatcttgtc ctttttctgc aattgaatca
    14581 gtaaaaatcg ttgaatagat aggaatattg gtatcttttg atactgtttt cattggtcta
    14641 tcatccacac tgctttctac gaataaggag ggaacttttg ttgtgcgtaa tttttcaact
    14701 aagtgtttta tttgatctgg tgttccttct tcttcagtgt tgatttccca aatgtaagca
    14761 gaaggcacat tatacgcttt cgagaaatat ttaaagcatc cttcacttgt aacaatcatt
    14821 tttttatcat tcggaattga agcaaattta gatttagctt ctttgtctag agaatccaat
    14881 ttttcaatat acttatctag attttcttta tagaattttt tattatcagg atctttttcc
    14941 gctaactgtt tttcaatatt tttagcgtaa ataataccgt tttctaaatt taaccaagca
    15001 tgaggatctt ccttcccttt ctcactctga ccctctaagt aaataacatc tatgccatca
    15061 cttgctgcaa aatagtcttt gttttcctct ttgttcgcat ttttaactaa ttttgtaaac
    15121 caagcatttc ctccagtttc caagttaaca ccgttataaa aaatcaaatc agcttttgaa
    15181 gttttttgaa catcttcagg caaaggttca tattcgtggg gatctttccc aataggtacg
    15241 atactgtgta aatcaatttt atcttttgct atattttcgg taatatctgc taaaatcgaa
    15301 ttagtaacta ctacttttaa tttttcgttc gaagccactt tatctttgtc tttactattt
    15361 attgaacatg ccccgaatat aaaaagagca atcaaaatcc ccagacttcc cagtactgct
    15421 ttccattttc tcatcataac gatctctcct tctttcttaa aaatttttgt tttggtgaga
    15481 ttagaaaact aattacaaat aagaatgccg ctgttaaaac aatacttgat cctgctgcta
    15541 tgttaaaact ataaccaata aacaatccaa gcaatgatga acttgctccg actgttgcag
    15601 aaataaatat catagttttc aagctatttg catataaata tgctgtagct gctggtgtaa
    15661 ttaacattgc aacaattaat atagttccca cactttgcat agctgttact gctactaaag
    15721 ttaatagaac catcaataga taatgataaa aactgacatt catccctatt gcttttgcca
    15781 ttaatggatc aaaagaagtg attaataatt ctttaaaaaa tagaactact ataaccaaca
    15841 ctaaaaccga aacgccaatt gtaatccact tatcaatatc ttgtacagct aaaatatttc
    15901 caaataaaat atgaaataaa tccgttgagc tatttgcaac tcctataagg ataactccta
    15961 gtgctaaaaa agaactgaaa gtaataccaa ttgctgtatc tccttttatt acactattgc
    16021 tttttataaa agtaataatg atagaagcta atagaccaaa tacaatcgca ccaataaaaa
    16081 aattaatacc taaaatataa gaaattgcta ctcctggcaa aacagcatga gaaatagcat
    16141 cacccataag agacattcct cttaaaataa taaaacaacc tactgttcca gaaacaatcc
    16201 ctataactac agatgtaatt agagcattct gtaaaaagtg aaagtcatac agaccattaa
    16261 taaaattttg aagcatatta ttcccccttg tatattagag ttagggtttt aaccttccgg
    16321 ttttgattgg ataaccctat gaaccatcgt tattttaatt tggtattaac tcatttcttt
    16381 tgagataata tatttattgg attgagaggc taagccgttc cccccttggg tcattgaacc
    16441 cgcactcgag agactggctc ctctctctga taagtggatt gcgtatgagt tagatgttga
    16501 aagtccttac tttactccgt atctcgtaca aggtagtgac gcttttcagt tgagtggatt
    16561 tcaatccaaa attctttttt tgaaaggtca gatatacatg tactttttag gcattgatat
    16621 cggtaaacgc actcacgttg cttctatcat gaacgaggaa ggaaaggtat tactaaaagg
    16681 tttttcattt cctaacacca ctgaaggagc agagtcatta attgagcgaa tggtcgacta
    16741 ttccggagca cccagtgact ttgttatagg gatggaagcc acggggcact attggctgtc
    16801 catcttttca taccttcacg aatcagatta tttgattcac gttgtgaatc cccttcaaac
    16861 agacggatgg cgtaaaggta cagaaatacg gaaacgaaaa aacgatatta ttgattctgt
    16921 tttgattgct gacttaatgc gctatggctc atttgtcgaa acggtcttat ccgatgaaaa
    16981 tgttttttct ttgaaacaac tttctcgtta ccgaacttat ttagtaggta cagcgagcga
    17041 cttcaaaaga aaaattatag ccgtattgga tcaagtcttt ccagaatacg cgacgatttt
    17101 taccaagcag ggcgttttcg gaaaagcttc taaagagtta ttgctggcgt ttagtgcccc
    17161 gatcgactta gaaaacattt cctcagaaac tttggcccaa tacttagctg agttaagcag
    17221 gaaccgaatc aaggcagaaa aagcggacca attgaagcaa gcagcttcta actcctttgg
    17281 agtaaaattt gctcaagaag cgttcacctt ccaactacgt tcaatgattg aacaattaaa
    17341 attcattgaa agccaaataa aggaaacgga aagagaaatc aagcacataa tggacacttt
    17401 aaattctgtt attctcacaa ttcctggaat tggtcctata aacggtgcga ctattcttgg
    17461 tgagatcgga gatattcaaa aattttctaa cccgaaaaaa ctcgttgctt acgctgggat
    17521 agacgcttcc gtgacacaat cagggcaata tgaggcaacg cataatgtta tgagtaaacg
    17581 agggtctcca tatttgcgaa aagccttgtt ttcggcggct ttagtagctt ctcaatgtga
    17641 tcctgttttt agtgcgtttt accagaaaaa agtttccgaa ggaaaacatc atctaactgc
    17701 cttaggtgcg gtttctcgta agctttgtta cgtgattcat gctattttga ctaaaaacga
    17761 gccttatata attgtttctg attagctatt gaacattcaa aaaaactaat ggtaagaagc
    17821 ttatttgttg tacccttttt tcggaattat ctgttataaa atattttttg atttggctaa
    17881 tttttacttg actctatata gttagtctct ctg
//
LOCUS       DAZABD010000053        17832 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000053 DAZABD010000000
VERSION     DAZABD010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17832
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.053"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1282)
                     /locus_tag="ACHALG_001995"
     CDS             complement(<1..1282)
                     /locus_tag="ACHALG_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN2587371.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVK"
     gene            complement(1580..2515)
                     /gene="murB"
                     /locus_tag="ACHALG_001996"
     CDS             complement(1580..2515)
                     /gene="murB"
                     /locus_tag="ACHALG_001996"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFN2587372.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(2588..3508)
                     /locus_tag="ACHALG_001997"
     CDS             complement(2588..3508)
                     /locus_tag="ACHALG_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFN2587373.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            3621..4373
                     /locus_tag="ACHALG_001998"
     CDS             3621..4373
                     /locus_tag="ACHALG_001998"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFN2587374.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            5237..5770
                     /locus_tag="ACHALG_001999"
     CDS             5237..5770
                     /locus_tag="ACHALG_001999"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFN2587375.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(6260..6784)
                     /locus_tag="ACHALG_002000"
     CDS             complement(6260..6784)
                     /locus_tag="ACHALG_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFN2587376.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(6777..7250)
                     /gene="tsaE"
                     /locus_tag="ACHALG_002001"
     CDS             complement(6777..7250)
                     /gene="tsaE"
                     /locus_tag="ACHALG_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFN2587377.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(7373..8353)
                     /gene="pta"
                     /locus_tag="ACHALG_002002"
     CDS             complement(7373..8353)
                     /gene="pta"
                     /locus_tag="ACHALG_002002"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFN2587378.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(8506..9186)
                     /locus_tag="ACHALG_002003"
     CDS             complement(8506..9186)
                     /locus_tag="ACHALG_002003"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFN2587379.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            9312..10181
                     /locus_tag="ACHALG_002004"
     CDS             9312..10181
                     /locus_tag="ACHALG_002004"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFN2587380.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            10207..10722
                     /locus_tag="ACHALG_002005"
     CDS             10207..10722
                     /locus_tag="ACHALG_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related/scramblase family protein"
                     /protein_id="HFN2587381.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(10769..11332)
                     /locus_tag="ACHALG_002006"
     CDS             complement(10769..11332)
                     /locus_tag="ACHALG_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN2587382.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(11387..11566)
                     /locus_tag="ACHALG_002007"
     CDS             complement(11387..11566)
                     /locus_tag="ACHALG_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587383.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(11643..12152)
                     /locus_tag="ACHALG_002008"
     CDS             complement(11643..12152)
                     /locus_tag="ACHALG_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587384.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(12248..12628)
                     /locus_tag="ACHALG_002009"
     CDS             complement(12248..12628)
                     /locus_tag="ACHALG_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFN2587385.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            12958..13707
                     /gene="pnuC"
                     /locus_tag="ACHALG_002010"
     CDS             12958..13707
                     /gene="pnuC"
                     /locus_tag="ACHALG_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFN2587386.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKLTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(13891..15168)
                     /locus_tag="ACHALG_002011"
     CDS             complement(13891..15168)
                     /locus_tag="ACHALG_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587387.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(15247..15891)
                     /gene="pcp"
                     /locus_tag="ACHALG_002012"
     CDS             complement(15247..15891)
                     /gene="pcp"
                     /locus_tag="ACHALG_002012"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HFN2587388.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(16090..17034)
                     /locus_tag="ACHALG_002013"
     CDS             complement(16090..17034)
                     /locus_tag="ACHALG_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HFN2587389.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(17036..17722)
                     /locus_tag="ACHALG_002014"
     CDS             complement(17036..17722)
                     /locus_tag="ACHALG_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HFN2587390.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
BASE COUNT         5184 a         3650 c         3091 g         5907 t
ORIGIN      
        1 ctttcacttg agaaataatg taatcggtat aagcagatcc ttgcgaaaaa tcaatacaga
       61 catgaatcgg tgcttcaaaa tatgataccg gacaaatacc gaccaataaa aagagataat
      121 cataaaataa gcgaatcatg aaacgttcat cttttcccaa gaaatagcgt ttattctctt
      181 gtatgacctt ttggattgct gtactgaata ttggatacgt ctcgaagaaa aactgtagct
      241 gctttttaga cgtaaagcta gtcatctcaa agtcgaatat ccaatttttt cgattgacat
      301 gcatcatacc catacgaagt tcttttcgta tatatagtct ttcttcttca gagatttcaa
      361 tcgaaaatgg taattgatca atcaattcca ctgacatttt ttgactaatc atgtggatat
      421 catgaaaatg agcttccgca agtccaaatg cgccatctaa ttcaaattct tcttccgtga
      481 tcaaaaacaa catgatataa gaaatctcat caatgatctg ctctttaggg atatgaagaa
      541 aagaaatatg tgataaatcc ttgatgatct tcattccttc tgagctctta aagtattcat
      601 ctgaaaagaa acgttttccg attggttgat tcccataggc cctcaacaaa ctaatagtgg
      661 ttagcaaatt gaatttgatt cgttttgttg cagacagttt taaattgaac agatattcaa
      721 aatattgcac gatactcttt gcttcattca tgacgctttc tctaaatggc atagaaaaac
      781 cattaaagac cccgaaatat accccaaata atacgttccg tatgtttatt tcttccccaa
      841 ctaatatgtt tttctttaac tttatttgat gctgttcaag aaaagagact aattctcttc
      901 tttttacata ggctcttgat atgcttaaaa aatgattctc tgcaaatttt tcgatcgtta
      961 cttttgaatc cacgatctcc gttaacagtc gagcaatttt tgatcgttga aagtagattc
     1021 ttcttaaatt tttgatatcc gtcaaattca gacgttcgac atggatctct gcttcttctg
     1081 aaatttggat ctctggcttt gttttgaatt cagtaaattc tttatttaat tctatgatat
     1141 aatttcttgt tttaaattta gacaaaccta atgtttcata taacttaggc agcactacat
     1201 aattcttttt ctcaaattct aaatatctag ctagcagcag tttttctcta tcttcctctt
     1261 ctataaggta atccaacttc atatcctaca cctcaaaaaa tgcctcatac caattacttg
     1321 atatgcagat gaaagttcat cttttcacct atactgattt tatgatggat tatgcttgtt
     1381 ttttcatcag ttattctcat tttttatctt ttttttgaga atattgcaaa aaatggaaaa
     1441 atttatgagg aaatattcta tattttttgt tttattgtgt taaaatgtac aagatttgcg
     1501 ccatttgcgt aaacaaccac aaaaaagagc aggacggcca ccgtcctgct cttttactta
     1561 ttttaagttc cattacttat taattggcta aacttctgct tgtttctgat attgcttgtt
     1621 tttctccaat gatccgcact tctgtttcta aagacacatc aaatttttct ttgattactt
     1681 cttggatgtg cgcaatcaat tcaatataat ctgtcgctgt cgcatgatcg atattgacga
     1741 tgaatccagc atgtttttca gagacttgtg caccgcccca tttcaggcct tgcaatcctg
     1801 cttgttggat cagctgacct gtatagtgtc ctactgggcg cttaaagaca cttccgcatg
     1861 aagggtattc taatggctgt tttgattgac gcaattcggt caactcatcc attcttgctt
     1921 tgattgcttc ataatcccct gtttgcaaac tgaatcgagc ttccaatacg atagcacgta
     1981 attcttggac tttactgtga cgataagaaa ataccatttc gtcttttgtc aaagttttca
     2041 atgttccatc ttcaagcaaa agatccactt cagcaaaaac atctttgatc tctccgccat
     2101 acgcacctgc attcatataa accgctccac cgacacttcc cgggatgccg caagcaaatt
     2161 caaatccagt caatgattct gccaaagctg cataagtcgt atcaatcagt gaggcacctg
     2221 cttccgcaat gaccatcgtt cctttgacat gaatctgttt catttcagtc agcatgatga
     2281 ctacaccgcg aatcccgcca tcttgaacga tcagattgct tgcatttcct aataccatcc
     2341 aaggtatatc atggattcgg caataatcga tgatttgctt tacttcttct tgttttttcg
     2401 ggaatgcaag gacatcagct ggtccacctg ttttcgtaaa cgtatagttc atcaatggtt
     2461 catcaaacaa tatagtaatc gtggctaagc tttcaactat ttcttcttta ttcactgagg
     2521 attttccttt cttttctcaa acgtcctcat tattctatca cgcttaatac aatcttgcca
     2581 gtcttgttca aagttgatga cggtagtgat ctttaagcac ttcctcacat aattcatgac
     2641 ttcttacaac ttctttttgt ttaagcaatt ctgtttccga tttacctaaa cggatctgag
     2701 aaaagaaggc ctctaccatt ggctcaaacc cgcgtttgac tagcgtgttt gtccagtcgc
     2761 caaattcttt cacttttttt ccatcagcat cttgaatgac aagctgggtc agatcgttta
     2821 cttcataagt accattttta cttgttactt gatatttttc tatattagcg ccacttgcta
     2881 aatccatcgt taaaatagcc gtttgatttt gtgtttctaa ttgtaaaatg gcatattcca
     2941 aaaattcctt tgtttctctt atctggtatt ttgttctcaa aatgggttca tctaataaat
     3001 acacagctgt atcaataaga tgtaaaaaca aatcataaag gacgaactct gtcgtttctt
     3061 ttgccgctat tcgatttttt tgcaggtgga tcaaccgctt atctgggata cgtttcaatt
     3121 cttctaccat aggagcaaag cgccggttga aaccgatcat caaaattatt tggttcttat
     3181 cagctaattc ttgcaattca cgaacttcat ttagatggac actcagtggt ttgtcaatat
     3241 aaacatggat accattttct agacattttt tggctaattc ataatgtact tttgttgctg
     3301 cgtggatcat acatgcttcg attttttctt ctaaaagctc atcgatcgtt tcatatagat
     3361 gttggaaccc gtactctttt tgaattcttt cttttgtttc tttatttctt gttgcaaaat
     3421 aaaaatcaag tgttccttgt tttttcgcat aagttggtaa atatgctttt tctgcgatat
     3481 tccctacacc gattacacca atcttcatct actttcctcc tggatcattt agttactttc
     3541 atcttacacc attttttact aaaatgaggt acaatagtat cgcaaaggta aggaaattaa
     3601 ttcgaaggag tgtgctttta ttgaaattta tctcttggaa cgtaaacggg cttcgtgcca
     3661 tcgtaaataa aaatttttta gaagtttttc atgagttaga cgccgatttt ttctgtctgc
     3721 aggaaacgaa actccaagca ggacaaattg atttagatct tccaggttat tatcaatatt
     3781 ggaattacgc ggaacgaaaa ggctattccg gcacagcgat ttttgctaaa aaaccagcat
     3841 tgaatgccac ttacggaatg ggtatcgata tccatgatac agaaggccgt ctgattactt
     3901 tggaatattc cgatttcttt cttgtaacct gttacacgcc aaattctcaa agcgaattaa
     3961 aacgtttaga ttaccgatta gaatgggaag aagcctttta taattatttg gaaaatctaa
     4021 aaaaacaaaa accagtcatc gtgtgtgggg atctaaatgt cgctcatcaa aagattgatt
     4081 tgaaaaactg gaaaacgaat caaaaaaatg ctggattcac tccagaagaa agagcggctc
     4141 tttctcgact gttggataac gggtttatcg atacattccg ttatttttat ccaacccagg
     4201 aaggtgtcta ctcttggtgg aactaccgat tcaattcaag gaaaaacaat gctggttggc
     4261 ggatcgatta ctttttgacg agcaaagatt tagagccagg actagctgat gcaaagatcc
     4321 atacggacat tatgggcagt gatcattgtc cagtagaatt agatttgaaa taaagaggtt
     4381 gtgaaaaagt cgtttagttt cgagtattaa gaaaaatttt gtgaaaatgg ctttccacat
     4441 tttgcataaa tttggcataa taccgagaaa ctggcttttg aacactgttt atcgagagat
     4501 tgtgaaaaag ctgtcctgca tcaagtaata agaacagcca aacaaaaata gttcctcata
     4561 tttttcgttt ggttgggctt attaccgcag atacaagctt ttgaacaccg tttattaaga
     4621 ggttgtgaaa aagctgcact gcatcaagta ataagaacag ccaaacaaaa atagttcctc
     4681 atatttttcg tttggttggg cttattaccg cagatgcaag cttttgaaca ccgtttatta
     4741 agaggttgtg aaaaagctgc actgcatcaa gtaataagaa cagccaaaca aaaatagttc
     4801 ctcatatttt tcgtttggtt ggacttatta ccgcagatgc aagcttttga acaccgttta
     4861 ttgagaagtt gtgaaatcag cgtcctgacc tgagtattag agaaacaaat ggcaaaatag
     4921 ttcctcatat tttgctattt tttgagctaa taccgagggt cagctgattg aacaccgttt
     4981 agtggagaag ttgtgaaaaa gtcgcctact ttcgagtcgt aagaaatttt tttgaaaatg
     5041 gttcctcaca actttgcata aatttgactt aataccgaga agtcatgatc cccatctaac
     5101 tccaaatcac aaggaatgag ttcaaaaact agcttttctc aacaaatatt acataaaaaa
     5161 ttctatgttt tttgttaaat ttatttgctt ttttcaaaag tatgttacag taatttccca
     5221 aggaggtttt acagaaatga attttgttgc catggatttt gaaactgcca atcatcagcc
     5281 ttatagtgct tgttctcttg cgctagtcat ggtaaaaaat agccagatcg ttgatgaatt
     5341 ctatacattg atacagcctg agactccttt tttctggcga aatgtccaaa ttcacggaat
     5401 ccatcaagaa gacgtacgca atgcgccgaa attcccagaa gtctggcaaa ctatccaacc
     5461 atattttcaa aaaaatcgtt tagtcgttgc acataatgct gcttttgata caaaggtctt
     5521 agcgggatgc ttagattatt atcatcttga acagcctaat tatctttctt tatgtacagt
     5581 gaaaagtagc cgtcgcctgt ttcctgaatt tccaaatcat cgattgaaca ctgtgtgcga
     5641 aaatttgaac atccaattag atcatcacca tgatgcattg gaagatagtc gtgcatgtgc
     5701 ggaaatcctg ctttatcaag aaaaaaaatt cgggacagat ccattgaaaa aacttgtgac
     5761 tataaagtaa aagaatactt atgaaacaca caaagaggtc gtgagcctgc cgtttcactc
     5821 cgagtcacaa ggagcaattt caaaaaatag cttctcatgt ttattaaata ttgtgactgg
     5881 tgaccgagga gctggcagga gaacaccgtt tattggagag gttgtgaaaa agctgttctg
     5941 catcaagtaa taagaacagc caaacaaaaa tagcttctca tatttttcat ttggttggac
     6001 ttattaccgc agatgcaagc ttttggacac cgtttaccaa gaggttgtga aatcagcgtt
     6061 ttgacctgag tattagagaa acaaacggca aaatagttcc tcatattttg ccgttttttg
     6121 agctaatacc gaaggtcagc tgattgaaca ccgtttattc aaaaaaaatg gactgcgaca
     6181 cgacttttgt cacagtccct tcgtgtgttt ttctgccttc tcaaaaggat gacagtttct
     6241 cactcttttc ctatttcttc tattcaatca aataactcat tttatataca tctaaaaatt
     6301 cacctagatc tgttcttgcc cctctaggca tgaccgcttc gatttggaag cccatttttc
     6361 ggtaaaggtg tacggctctt tcattacgaa cttgaacgtc aagctccaat cgaaaaagta
     6421 cacccatttc cttcgcccaa ctgatgattt cttctagcat aagagtgcct agtcccattc
     6481 cccaatattc ctgcagaata ctgataccga cttctcccac gtgagacaaa cgaaattgac
     6541 tgtctgcctt gacagatgct gttccaataa tcgtcccttc atagattgca agcagcaata
     6601 aattattatt gctttcgtat atataatcca actcttcttc taaggtttct ggtggtaaca
     6661 ggagttctgc ttcatccaaa actaaaaatt ctgtctcctt attaacttgt cccatcatca
     6721 aaagaatgtc ttttgcatct cctggcactg cttcccgcaa gatgatccca acttcttcac
     6781 tcatgatttt cagcccattt ttctagaatt cgctgtttga aggtttcact ttgctccccg
     6841 tatgagatta gtttcacata ccgcttttct aaatctgtat cacttttttc taaaatcagt
     6901 tccaaataat cctccggcag tgcttcttct aatagatttc cccattcaac aacggaaagc
     6961 ccatcacctt caaaatattc atctaaccct aaatctgctc cgctagctgc tacccgataa
     7021 atatccatat gatacaacgg aatccgacct tgactatatt ctctaataat cgtgtatgtc
     7081 ggacttttga tcatttgcgt tatccctaat ccttgggcaa ttcctttcgt caacgttgtt
     7141 ttacccgcac ctagatcacc agtcaatatc agattgtcac ctggcattgc ggaatgacca
     7201 attgcctctg cgaattgttc tgtcattgtt aatcctgata gttcgatcat ttttcatctt
     7261 ctcccgctct tctttctttt tgccagtata tcgcgtaatg aagttttttt aaagaaaaaa
     7321 tgaactagtc aagaacagca gctgctgttt ttggctagtt cgcgtgtatt tgttaatcaa
     7381 tcaatgtttg tgctgctgtg ataatcgata atttatatac atcttcttca tttgctccac
     7441 gagacaaatc agaaaccggt ttgttcaagc cttgtagaat tggtccgatt gcttcaaagt
     7501 taccgaaacg ctgagcaatt ttatagccga tatttcctga ctgcaattct ggaaatacaa
     7561 agactgttgc ttgaccagct acttctgaat ctggcgcttt cagttgtgca actgaaggaa
     7621 cataagaagc atcaaattgc aattcgccgt cgatcatgta ttgtggcgct agttttttag
     7681 cgattttagt tgcttcaact actttgtcta cttctggcga tttcgctgag ccttttgtcg
     7741 agaagctcat cattgctact tttggttcga tatcgaacaa ttctgctgtt tttgcagaat
     7801 ctacggcgat ttcagccaat tcttgcgcag ttggattcac gttgatcgca caatccgaaa
     7861 agacatattt ttcttgatct cttccacgta ccatgatcat cgctccgctt gtccgactga
     7921 tgccaggctt tgttttgatg atctgaagtg ctgggcgaac agtgtcgcca gtcgaatgga
     7981 tcgctccaga gaccattccg tctgcaatcc ccatatacgt aagcatcgta ccaaagtaat
     8041 tcacatcttt taaaatcttt tctgcttgtt ctttggatgc ctttccattt ctacgttcaa
     8101 caaaagtatc caccatttca tcccacttat cgtaattatc agggctgatg atcgtaaagc
     8161 tagatgtttt gattcctctg gcatgcgctg catttcttat ttcttcttcg ttccctacta
     8221 aaatgggttc cacaagttct tctgctttca accgggcagc tgcgcctaaa atacgagcat
     8281 cagtagcttc cgggaaaacg attttgatcc cacggcgaac aattttaaat cttaaactat
     8341 caaacaattc cacaagatga cctccagacg tttttatatg tattatcata cactattcca
     8401 gaaaaaaaga acagtttatt ttatctgtat cagtcaaaaa tgctggaaaa attaagtttt
     8461 tcacatacaa agcgaactgc actataacag gaaagcgctg ttttattaca ctgtttcagg
     8521 cagttgccaa tcaatcggtt cttctcctaa cgcaaggagc gcatcattcg ttttcgagaa
     8581 gggtttggaa ccaaaaaagc cacgacttgc agataacgga ctgggatgtg gagatgttat
     8641 gatgatatgg cgactcttat cgatcatttt gattttttct tgtgccggtt ttccccacaa
     8701 gataaacacg attggttttt cccgttcatt taatttttca atgatcgtgt ctgttaatct
     8761 ttcccaacct ttcccgcgat gagaatacgc ttgcccttcc cgaacggtaa gaacggtgtt
     8821 gagtaaaagg actccctgtt tagcccatga cactagattt ccatgttgaa caggtgcgat
     8881 tccaagatca tcataaagct ctttatagat atttcttaac gatggcggga tctttacgcc
     8941 aggttgcaca gaaaaagata agccatgagc ttgattttct ccatgataag gatcttgtcc
     9001 taaaatcacc actttaactt cttcataagg agtcaattcc aatgcttcat agatatgata
     9061 catatctgga tggatctttt gggacgcata ttcttttttt aaaaattctc ttagatgcag
     9121 ataataatct tttgaaaatt ctgaggacaa aacttcttgc caactgttat gaatgatttc
     9181 tttcaaaaac tccacctcct aataaaaaga ataactcaca ttttcttcaa tcacaaggga
     9241 caatcttcac cctcagcact tgattaatat ggtaaactag gaacaagaag cagaaaggaa
     9301 cgataatcat tatgatcaaa ttaattgcat cagatatgga cggcacatta ttagatgccc
     9361 atatgagtgt ttctcctgaa aatgccgagg cgatacgttt tgcaaatgat gccggtgttg
     9421 agttcatggt cgctactggt cgaaactatc aagaagctcg cgcagcttta gatgaggtag
     9481 gaatcgattg tgcgatgatc actttgaacg gggcacaggt atttgataaa gaaggaaatt
     9541 cattgtttac cgtgtcgatt ccaaataatc aagcaatctc tgttttagat attctagatg
     9601 ctaacggtat ttactatgaa gtagcaacca acgatggtct ttattctgaa aatcaagcga
     9661 aacgaattga aagttttgct tccatggtgg ctacccattt acctcacctt acgtataaaa
     9721 tggcgattgc catggcctca gctaagctgg agttattgca catcacatac gtggacagta
     9781 ttcgtgctat tttggaagaa aagaatctgg aagttttaaa aattatttgt tttcatacag
     9841 aagggccagc tgtattaggt ccagttggaa agcagatttc tcttttaggc gatctagccg
     9901 ttacttcctc tggtcaaaac aatatcgaag tgaaccataa aaacgctcaa aaaggaattg
     9961 ctgttgccca tgtcgctcac gagcgtggga tttctttaga agaagtcatg acgattggcg
    10021 ataacttcaa tgatgtcagc atgctgcaag tagcaggagt cagtttcgca atgggaaatg
    10081 cagaaatcga agtaaaagat tatgccaaat atacaacgga cacaaacctt gaatctggcg
    10141 taggaaaagc catattgcgc gcgattgcag aagacttgta gttttatcta gaaaagaggg
    10201 atatagatgt cagaattttt cattcaggaa aagcagcttg gacgtgcaac tcggacaatt
    10261 gtcaaaaacg aaaaaggaca ttccctgttt ttgatggttg gtcgctgggg aacaaagggc
    10321 gatgcattgt ctctttatgc catgaatggc gatctcgttg ctcacatcaa gcagataagc
    10381 ctgacattcg gtacgcgttt tgagctgtat aaagagttcg aaaaggtggg aaccatgcag
    10441 aaaatattca attggcccgg tgacttttat tatattcgta gacttcactg gacagtccaa
    10501 ggtgatatct ataatcatca atatcaaatc catcatttca atcagcaaat catgaaaatg
    10561 gataaagcaa cacttttttc aggagattac tatgtattgg atgtctcaaa tgatgaagat
    10621 gctccagttt gtatttgtat tgctgcggtt ttagattact ggctctaccg taaagacaaa
    10681 agtcgcgata agcctgtttc ttaccgactt aacttaagtt aaaaagatgt acctgaaatg
    10741 cggaaatacg catttcaggt acatcttttt agatattctt ttgcatatga tcataaagat
    10801 gaccactgat catccgctga ccgacaactt tgaagtcatg gcgttcgtat aattttttag
    10861 cctttggatt tttttcatcc acacttagac cgatgacgga gcgattagct ttcttcgcca
    10921 atttaggtaa tgcttctaat aatcgcgaac cgatgccttg tcctctaaaa tcttctgata
    10981 cactgataga gtccaaatac cattcgttgg gaaacgtttc tttatcaatg aacaaacgtg
    11041 tagacgcatc tagctgatat ttctctaaga taggtaccag tggctgatcg atgattgatt
    11101 cttcttcatc tgtataaccg taagcaactc ctgcaacttt accgtctatt tcttctacga
    11161 tacctcgtga ataactgtaa cgataatttg gatcagtgat tgcttcttct aaaatctcta
    11221 aagtcttttg ttcccctgtc tgcttcaaaa atggcagttc catatctttt agaatcacta
    11281 agatcaatgg ggcgatttct ggagcatctt cttttctcgc aaaacgaatc atagtttcct
    11341 ctccttttct ccattctaac atgcagaaaa agaaatcgca aaatcgttat tttaaaaaat
    11401 cttcaatctt ttcagtaatg aaataaccat gagtaacgat tcttgccgct tcagttcggc
    11461 aataagggca gacgactggc atttctttat cattatgttc gactacatta ttgacttctt
    11521 cgtatgcaaa tttttttccg caattagggc atttcatttt ttccatgtta tctcaacttc
    11581 ctttcatctt tactctctta gcttactctt atattgtaaa aaagagaagc aatatccttc
    11641 ttctatgcag atatccatac ctgacggtcc tgttctccat ctattaatcg gactttataa
    11701 taaggacgtt catctttgac tagaagtgtc cactctttca cttttgcttt tttcccgtag
    11761 tagctgactg cttccttcat cgctgcttgc ggttgtttga actcttccgt tttttctata
    11821 tcaaaaagat gttctgcggc cttttccgtg tcatttcgta cggttgtttt cccagtggat
    11881 ggattgactg ctatgacttg tccttcacaa aaaaactggt attcatatgt tgcattcgta
    11941 ggattctcca aatctttttc aactgcaaaa tgaatagcag atatgttatg ttcttttgtt
    12001 tcgtttttta cgattgctgc tgcttttttc aatgagatcc gatattcctg gtcactaata
    12061 tcatccatgt gaaaagcggc attgagcagt cccgtcatac cggtaatccc tattacgatg
    12121 agggcagcaa caccaaacat catttttttc attcttcatt ctcctgtttc ttttttttaa
    12181 agaatatcaa gaaatgaacg aatgaaaaat cgaatgaaac gaaaaaaaag tgacaatttt
    12241 gttatcttta ttcgtgcagt tctaatggca atccgtctgg atcaaagaaa aacgtcattt
    12301 tttttcctgt aaaggtatct tctctaatcg gttctgattc gatccctaag gactgcaatt
    12361 ctgcaattgt ttcttcaata tcgtctactt tgaaagcaag atgacgcaag ccacaagctt
    12421 ctggatagtt tggtcgttgt ggcgcattag caattccaaa aatctccaat tcagcacttc
    12481 ctagtttcag atctaattta tagtcatctc gttctttgcg gtaattttct ctgattactt
    12541 caaatcctaa tttattaaca taaaaatcac gtgatttctg ataatctgaa acaatgatgg
    12601 ctatatgatg gatcgttgta aggttcatat gagcactcct attcttcgtt taagctcgga
    12661 tctatattaa tctccagttc cattgtaaat gtgtcagatt gaaaaaaata gctgtttttc
    12721 ctttttataa gaaaaaaggc aaaaaaaaca gaaagtaggt cctccaagac actcttcctg
    12781 ttcccaatat aataaatgaa agtaagaaat tatattttca agaaaacaaa cagaaaatta
    12841 tcataacaca aattatttat aattgaaaga ctagtttttt ttcaagaaaa gcatacaata
    12901 gattgtgtta gtaaatacag aacacactac atttagtgtc ttaaggagtc ctcatacatg
    12961 aagtcatatt ttcagttttt agctcatcag ctaaaaggct ggccgcaaca aaattattat
    13021 cttttctttt tcagtcttgg ctgccaggtc atgactcttg tcaatagtcc catcacttgg
    13081 ctgtccgtta ttacatttat cggtaccacg cttggtgtat tgtgtatttt atccatcaat
    13141 gcggcaaaat cagtcaacgg tattttaggt attttgtctg cgatttgttt tattatcgtt
    13201 gggttttcag caaagaacta tttaagtatc ggtgaacaga ttgcttatat gatcacattg
    13261 gatatccccg tattgttaag taaagaatgg aatcataata tggcttcaaa aatccgaaaa
    13321 ttaactggaa aaagctggtt tatcgcaatc atcagtactt tccttgtcta tcttgtttca
    13381 ggattcctga ttggtcgtta tacagatgat cctcggccgt gggtagatgc cattgctttt
    13441 tctattagct tgactgctgg tatcatcagt ttcttgcgtt acaacaacca atattactgg
    13501 tggttagctt cagggcttgc tcaaatggtc ctttggtata tttcatttac gcatggatct
    13561 gcaagtttgg cgatgttcgt gaacagctct gtttacttgc tgaatgatgt ccttgcattt
    13621 accgtttcac cttggtataa ccaaaaagaa cgtgaacgta tgcaaaaaat ggaagaagaa
    13681 tactacaacg cacaacaaca cgcataaaaa aactgagcgt gggacaaaag taaagaacac
    13741 ttttgtctca cgctctaaac acgtataaac ggcggaatca gaagcaactc cttcggaaat
    13801 aagctgaaat tcacaaaaat ttgaaaagca attttcgtga atttcttctt atttcttgga
    13861 gttaaacgct tctgtcccag cctctatttt ttatagtaat tccttcagat actttcttgc
    13921 ctcatcattg aaacgcagtg attcttctaa cttctgataa tattccaacg cttgtttctg
    13981 agcttcagta aaacgataat gttttccttt ccatttgtcg attgtctctt gcacatttcc
    14041 agattggata tctttcacta ctagttgcca tttttctttt gagactgtac ggtctttctt
    14101 atcagcagat tgtatagctt ctcgttcttt acgcaatact tttaaaaaac catttctgac
    14161 tatccgagga atgctgtcga agaacccttc tgtaaaacca tagcgacaat attgcttcca
    14221 aataaataga tagatttttg gttggatgaa tcgttggttt ttcgcaaaat attccatctg
    14281 agcagtgact tctggtgttc gttgacgttt tgtctttctc aacacggaaa taaatgtttc
    14341 aaaataagca ttaggatgta ctggcacttc ttcatatacg atggattgcc aatctttacg
    14401 taaaaatgcg cgatagttga tcaagaattc ttctacatcc tttactaatt cttctgcaaa
    14461 gtcgtcaaat agttcttttt ttatctgtcg cttttttgac tgcccattga tgtgttcact
    14521 tattttcttg atttcatttt ccaatgcttg ataatccgct atgtaaatcg gcatttctac
    14581 ttggcgaaat tctgcgagcc gctgagctaa ttttatatcc ggtaaatatg gctgtttcat
    14641 ccgacgattt tggtacaaga caaaacagta tttttgccat agtccttcag gaaaatcgat
    14701 attcttctgc ttaagccata ataattcgtc taaggccaga tcaacttttg tcattccctg
    14761 atggatcgaa gctgccactg tcggagagac atgcaggtga tcggcaaagt ggttgatgcc
    14821 tagttttagg atctttccgg tttttggcga tttaacgatg tattgcactt ttaattctct
    14881 accacactca caatacaatt tttgctggct tcgttttgtt cgaaaaaacg gattgatctt
    14941 gaagtcagaa aacacccaat ctgttgcagc taaataatgt tcttccataa aacgagagct
    15001 gattagatat cggcggtgct tatcgactag ttcccgctgt tttttcgtta attttgcata
    15061 gatctcccaa cgttgactgc ttgtgtattg tttagaaatt ttgatgggat agcttttgct
    15121 gatttctttt ggaaaccatt cttcaaagac ggattgccat tcttccacgg ctgtttcttt
    15181 cctttctaat tacaaaaagc tggctactaa cataacgtta gcaaccagcc agtgtttctt
    15241 gctaaattag tgagttgttc cggcatttcc ggaaatatct tctttatttt ctactgctgc
    15301 ttcaactgca taagtcaaag aagaagcaat gtcttctaaa gacatagaag gcgtacccac
    15361 tggacgatcg atcacttgat cgggtgagaa tggcacgtgg atgaacccac ctcgaattgc
    15421 cgggaattgt gtttcgatca aatgaagcaa acgatacatg atatcattgc agacaaatgt
    15481 tcctgctgta taagagatgt acgctggtag accatgatct tgtacatttt ttgtcattgc
    15541 tttgattggc aatgaggtaa agtaagcggc tggaccgttt tcttctaaag ccgtatcgaa
    15601 tggctgattt ccttcattat cagggattct tgcttctgct agattgattg cgacacgttc
    15661 gaacgagact gctgaccgcc cacctgcttg tccgacacag atcacgacat ctggttggtg
    15721 ctgttggatg gcttctttga ctacttgact gcttttttcg aatacggtcg gtacttccac
    15781 tttgatgatt tcagcaccgc tgatttcatc tggcattttt tttactgctt cataggctgg
    15841 attcactttg tccccaccaa atggatcaaa acctgttact agtactttca tctgttgttc
    15901 ctccgtccga ttgatttaac atatggttat cttttatcat accattttcc ttgaacagcg
    15961 taaataaagg aaacaatagt ttaaataaca taacagatca aacttaatat attcttataa
    16021 aaaaatagtg ttccactaca atgttagaag aggttgcgac aattattctg tcacaacccc
    16081 tctcttctat tatttaaata gaatcatata agcaatttgg aaaactagca taaataacgc
    16141 aataaacaac tggtttttga tcactccgta tttgtctttc atctccagca tcgctacagg
    16201 cacgatattg aagttagcag ccatcggtgt cagcagggtc ccacaaaaac cgcaagtcaa
    16261 tgcaaccatt cctattaacg ctggattcgc tccatgacta aatacgaatg gtgcaccgat
    16321 cccaaccgtc atcacggtga tggccgcaaa tgcattcccc atgattaccg taaatatcac
    16381 catgccaaga gcgtaaatga tgatgccgat gttcacattt ccttctggaa cgatcgtacc
    16441 tactgcattg gaaatcactg tcccaactcc agcgcttgta aataccgcac ctagagaagc
    16501 taaaagcatt ggcaacatgc ttaacggccc tactgtgccc agcatatctg ctgcatcatc
    16561 taaaaatgta cttggtcgat tatctcgaga aaagaacatc aagattagca tggctgcaaa
    16621 aacaccgacg cccacgccga ctaaagcacc taaattggta aatagagcaa acaagatcgc
    16681 aaagacaccg atactcaaag ctggaataaa aattttcatt cctagtttgt ctgacatttt
    16741 ttccatatat tcttttgatg gaagccggct ttctcctttg ctgactttct tagcaattgc
    16801 tggtatggtc atcgcaaaga tcaagccacc gttcacgatt gccggaagcc atcttcctcc
    16861 tgcaataact acccctaaga ccgtccagaa aaaggctgac ccataacgat gaggattggt
    16921 tttatctgtt aaatttctta ctccagtgta aatcaatacg agtcccatca cgatataaag
    16981 gatctccagt aatttatcgc ctaataaaat atcaggattt gtaaaaaagt ccatcttatt
    17041 gcccctttcc tggataatat ttcttcgcta atttcttttc tttaagatag aagtagacac
    17101 ttgccacaac taaagccgtg atagccactg gaacttccac taaagcaagc tgtcccaatg
    17161 ttacgtcata tcctagatct ttcagtgttg attggactag caacgcccct gctcctccga
    17221 tgaatagtac ttggccaaag aaccagcaga cattttccat agctgaagcc atacctttta
    17281 gttcttcttc atgcttagga tggacttcta acccttttga ttcgatggtc cccaatgcca
    17341 tcggtaaaat gattggacga acaaatccag caactccacc aaaactcacg ttaaaagccg
    17401 caaagatcat tcgaaagatc ccataaatat ctacaatcat accagcagat actttcttga
    17461 atcgcttgat caaggaagcc gctgattctt tcagaccatt tctttcaagc gttcctgttg
    17521 ccaacataat gatgataaaa atcgccatcc cgcgattgtt tacaaaactt gtacctaaaa
    17581 gttctaacat tccttgaatg ccaagaccgc cagtcaaagc tgtaacaact aaagcaacca
    17641 ttacgatcaa tatcgagtct aatttaaatg caaacccgag aataacaatc aagacgccta
    17701 aaaggctaag ataattttcc atatattttc ctccataaaa tgacaatatt tttttaatta
    17761 taaagcaaaa cacataaaaa atatagttgt tttctcacga aatagacact atttttaaat
    17821 atttttcgtt at
//
LOCUS       DAZABD010000054        17255 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000054 DAZABD010000000
VERSION     DAZABD010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.054"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(194..739)
                     /locus_tag="ACHALG_002015"
     CDS             complement(194..739)
                     /locus_tag="ACHALG_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFN2587391.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(751..1443)
                     /locus_tag="ACHALG_002016"
     CDS             complement(751..1443)
                     /locus_tag="ACHALG_002016"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFN2587392.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1462..1746)
                     /gene="macP"
                     /locus_tag="ACHALG_002017"
     CDS             complement(1462..1746)
                     /gene="macP"
                     /locus_tag="ACHALG_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthase accessory phosphoprotein
                     MacP"
                     /protein_id="HFN2587393.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1759..2322)
                     /locus_tag="ACHALG_002018"
     CDS             complement(1759..2322)
                     /locus_tag="ACHALG_002018"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFN2587394.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2550..3212
                     /locus_tag="ACHALG_002019"
     CDS             2550..3212
                     /locus_tag="ACHALG_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HFN2587395.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3336..4532
                     /locus_tag="ACHALG_002020"
     CDS             3336..4532
                     /locus_tag="ACHALG_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HFN2587396.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4633..6009)
                     /locus_tag="ACHALG_002021"
     CDS             complement(4633..6009)
                     /locus_tag="ACHALG_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFN2587397.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(6046..6912)
                     /locus_tag="ACHALG_002022"
     CDS             complement(6046..6912)
                     /locus_tag="ACHALG_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFN2587398.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(7001..8026)
                     /locus_tag="ACHALG_002023"
     CDS             complement(7001..8026)
                     /locus_tag="ACHALG_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFN2587399.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(8044..8718)
                     /locus_tag="ACHALG_002024"
     CDS             complement(8044..8718)
                     /locus_tag="ACHALG_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFN2587400.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8902..9795)
                     /locus_tag="ACHALG_002025"
     CDS             complement(8902..9795)
                     /locus_tag="ACHALG_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFN2587401.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9869..11566)
                     /locus_tag="ACHALG_002026"
     CDS             complement(9869..11566)
                     /locus_tag="ACHALG_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFN2587402.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11559..12872)
                     /locus_tag="ACHALG_002027"
     CDS             complement(11559..12872)
                     /locus_tag="ACHALG_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFN2587403.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12872..17141)
                     /locus_tag="ACHALG_002028"
                     /pseudo
     CDS             complement(12872..17141)
                     /locus_tag="ACHALG_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333417.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
BASE COUNT         4884 a         3854 c         2974 g         5543 t
ORIGIN      
        1 aaaattataa taagtgtatt ttataaaaag aaaaaatata acttaagttg ttgataataa
       61 agagatttat taacgtaaaa agaataagtt tttttaaaat aacgtataaa aaagagcata
      121 aaacaaagtc gtttgtttta tgctcttctt ttattatttt gtgcttttta ttttatcaaa
      181 tcactttgtt tttttataaa atttctgcgc ctagtgtgga ctcaaaatgt ctcaatgccc
      241 attcatgtcc aacgggatcg aaacttgcca cggcatcttt gaaaatatgg agtttgtatc
      301 ctagattata cgcatcgact gctgtatgca acacacagat atctgtacat acgccggtca
      361 aataaatatc tgtgatctgg cgttctcgca aacggatgtc tagatctgtt ccactaaaag
      421 cggaataatg ccgtttgtct atccaataaa cattttcctc ttgctcatag tcttggtata
      481 aactttttaa agagccgtac aattcccgtc cacccgttcc tatcacatta tgcggaggaa
      541 ataagcggtt ctccggatga aaggaatctt ttggatcatg cgcatcaatg gcaaaaacga
      601 caaaatcctt ttgatcaata aacttctttg tatgagcagt cagctttttt tcaattactt
      661 gtcctgctgt cccagtagtc agctttccat cctctgcaac aaaatcatat gtgtagtcaa
      721 tcgagatcaa tgcgttcatc ttagtcctcc ttatacgaga tgtttgacaa attcgatcac
      781 catttctgct gatcgtttgc ctgcgtcttc gataaattcg tcaaagcttt gtgtcgccga
      841 gtgatctgct gtgtcgctca ttgcacgtac gatcaaaaat gggatattga actgacgagc
      901 tgtttgtcca acagcggctc cttccatttc gcaagctaat gcttcaggga aattcgttaa
      961 gatttctttg attttatctg gcgagtcgac aaatgtatca cctgtaacga tcaatccttc
     1021 ttttgcgttc aaattcgttg cttttgctgc tttaaccatt tcactgcgca aatatgtgct
     1081 tgcttcataa tatagaggca ttcctggtaa ttgtcctggg ttgtagccaa atcctgttgc
     1141 atctgcatca aaataagcta ctttgtctga tataacgatg tcacctactt gtaagccttc
     1201 accgattccg ccagctgaac ctgtattgat caccatgttc acgccgtatt gttggatcaa
     1261 cagacttgtg gtaatagatg ctaagacttt tcctataccg gaacgtacga cgatcacttc
     1321 gtggttacct aatgaacctg aaataaataa agcgcctgcg cgttcccatg ataatggttc
     1381 gcttaggttc tctctcaaaa tttttacttc ttcttccatt gcgccgataa taccgatttt
     1441 catcttttta attccttctt tctatataaa tgcgattgct aaaaaaacca cacataacat
     1501 taggataaca ataataagag atttcatcaa aaaagagttc catttggtcg tcttttcccg
     1561 ttcgctgatc cttgttttcg tgattggttt attctttttg ctttctttac gataaaagct
     1621 ggcaatcttt ttttcttctt gctgataagc tgtttctgct tttcgctgtt tttttaatga
     1681 ctcgcttgct tgtgcttgtt gtcgcttgcg aagttcgctt cgcgtaataa gcggtccttt
     1741 ggccataagc cgatccctct actttccttt ggtcaatagt tcccagtatt gaatcgcata
     1801 aatcgtcttt gcatcacaaa tcaactgatc tttcatcgct tgttgcgcct cttcaagagt
     1861 tagatgatac aactccagta cttcgtcttc atcttgtgct aaaggattct cgaccttctc
     1921 taccccttgt gcatgataaa tatgaaggac ttcatttgca aaacctggtg aaagatacat
     1981 cgaagtaaga tggctcagcg acttcgcacg atagccagtt tcttcttcta attctctagc
     2041 tgctgtcatt tcgggatttt gtccttctcc tggatcgatt tttccagctg gaatctccaa
     2101 aatcactttt tctaaaggtt ttcgaaattg tttgacaaga agcagacgat cttgttcgtc
     2161 aaaagcaatg atcccgacac cgccaggatg aaataccagt tctctcttcg aagttcctcc
     2221 atctggcaaa cgaacttcgt ctacagcaac atcaataatt tttccgctat agatttcttt
     2281 gcgactgatt gttttttctt caaattgctc attgttcagc atatcttctc ctgctttctt
     2341 tgtaatcata actgcttttt tttcattttt caatttttct caaaaactcc ttattagttt
     2401 accatagata aaccttagat tttctctaaa tatagaaaat ccttctttag tccgatactc
     2461 tttatagtag tccgtatgct aaaataagta tgtaatcgaa taacgaaaag aattcaatac
     2521 tcaacgataa atgacatgga gggtttatta tgataaaacg aaattggccg tggcttctag
     2581 cattggtctt gctatttctc tttgtccgaa acaatagttt ttcttttgtt ctaggacttt
     2641 tattgatagg tgtagtttta gttttgtggg gactctttgg ttcgcgcagg aaaaagggaa
     2701 aacaagaaga aattcctgca ctgactgacg aacttgaaac tcactatgaa aaaagcggta
     2761 tgacagcgaa tgaaatttca ttttttcgac aaacaatgaa tcaaacaaaa aatgagatca
     2821 atcaattgca aatgaatatg cagcagacag ccaaacttaa agcaatcgat ctccgccatg
     2881 atccagtcaa agcagcgaaa gctttattca aagaattggt gaaagaacca aaccgactgc
     2941 atgaagcaag tcagtttctt tatacgcatc tgccgaatct agttgatttg acaaataagt
     3001 ataatgagat caatgaccat gagatcaaaa acaaacagac ctatgaaaaa ctagaagaaa
     3061 gcgcgcagat catcgaccag ctgtcagctt taatcgcaca agattatcaa aaattcgttg
     3121 ctgaggatct agatgatatc gacgtagaga tatctgttgc gaaacagagc ttaaagcgtg
     3181 ataataaata tgaaccagaa caaaaggatt aactcttttg ttcataaaag aagactcgga
     3241 acattcagat gatgtctggc tgtttcgagt actttttcta aagtagagac attcatgtaa
     3301 gaatcgagaa cagattcaac aaggaggaat tttttatgga tcataaaccg actgaaacaa
     3361 aagatgttac accagttaac gatacacttg aagatttatt gaacaacccg ttttcaactc
     3421 caacagatac gctgaccact tcacaacaag cggagattga tcagctccaa gagcaacaga
     3481 cagctgctcg tttaattgac aaactaccgg cggaacgcca agaacaagct aaacagctag
     3541 cagcaaaaat cgatgctaat gatgcacagt ctgtcatcag ctacggatcg gctgcccaag
     3601 caaaattaag tgaattctca caatcgatgt tgaatcatgt gcaagcacaa gatattggtc
     3661 ctgtaggaga ttccttaact gaactgatgt atcgtctcca agaagccaat cctgacgaac
     3721 tgcgtgccgg agaagggaac atcttccagc gtgtcttcgg gaaggtcaaa caatcgatct
     3781 atgaagtgac agcaaaatat caaaagattg gtgcacaaat tgacaagatt tcagtgaaat
     3841 tagataaaga aaaagacggt ctgctaaaag ataatctgat gttagaacag ctttaccaga
     3901 aaaacaaaga ctactttgat gcactgaata tttatatcgc tgctggagaa ttgaaaatgg
     3961 aagaacttca aacaacgatc attccagaag cgatgaaaag agcagaagaa actggcgatc
     4021 agatggatgt tcagattgcc aatgattaca cgcaattcct agatcgtctg gataaacgta
     4081 cgcatgatct tcgcttagct agacagatca cgatccagca agcaccacaa atccgtttga
     4141 tccaaaacac aaatcaggcc ttggctgaaa agatccaagc atctatcgct acagccattc
     4201 cgctgtggaa aaaccaagtc gtgatcgcat tgaccttgct tcgtcaaaaa gatgcagtaa
     4261 ctgctcaacg ccaagtctct gaaacaacaa acgacttatt gaagaaaaat tcagaaatgc
     4321 tgaaaatctc agccatcgaa acagcgaaag aaaatgaacg tggaatcgtg gatatcgaga
     4381 cccttcaaac cactcaaaat gatttgatcg aaacaatcca agagacattg cgtatccaaa
     4441 aagaaggaaa agaaaaacgt cgtcatgcag aagtagagct tggacacatg gaagaagatc
     4501 tgaaacaaaa actgttggat ttaacccaat agatcacctt tcgcttgtta ctaaaacaaa
     4561 cgcccaacaa cagtttatta caactgttga tgggcgtttt tcttatcttt cttctaactt
     4621 ctccattgga gtctatttct cattcatctt gctgtgtcca tagccatagc caaaataaat
     4681 gatcagtcca ataaccatta cgataccgaa cacgatccat gtcacagttt gcagacgaag
     4741 catcaataca atacaagtca agatggagac gattggcaat agcggaacga atggcacttt
     4801 gaattctcca gcttttggtt tgccaaacat ttttctcaac ttcagtaagc ctagactaac
     4861 aagcatcaaa taggcgagcg ccatgatatt cgttagttcc gctaatacat tcaatgggaa
     4921 aaatccggca aagaatacag ctgccacacc agcggcataa gtcgcttttt tcggtgtacg
     4981 atgcttttga tcgatttctg tcataaactt aggaagtaag ccatccttgc tgacagcaaa
     5041 cagtaaacga gcaagtgaat acatcatcga gatcgttacc gtcagtagcg ttaaaattgc
     5101 ccctacagcg atcaccgcgc ccactgttcc atgtcctaca tacctcattg caaaagccac
     5161 tgggtcattg actcctagtt cagtatacgg aacgattcct gtcaagatca gcgtcacgat
     5221 gatgtaaagg acagtagcaa tcagtatcga accaatgatt cctcttggca catctttttg
     5281 cgggtttttg acttcctcgg ctgccattgc aacggcatca aatcccaaga aagcaaaaaa
     5341 cacaagagct gcaccatcta gcacaccgga aaaaccaaaa ggcatgaatg gctgccagtt
     5401 atcaggttta acgaaaaata ttcctacaat gataaacaac gcgatgatac caaatttgac
     5461 atataccatg gcattattca aacgtaatgc tttcttggct tcaagcgaca cgatgtaagt
     5521 gacgaatatc actacgagca ccgcaatcaa gtcaatgtat gtaccatttt ccggattgta
     5581 cccgccagtt aatgcttgtg gaagttcggc ccctaaactt ttcaaaaaac cttgcatata
     5641 gccagaccat ccggaagcaa cagaggaaac cgctagtaaa aattccccga tcaacaacca
     5701 ccctgtcagc caggcagcaa attcaccaaa tacaacatac agataggcat atggcccacc
     5761 aatcactggc actcgcgaag caaattctgc gaaacttagt ccagacaaaa tgacaacgat
     5821 tgcagctaag acaaatgaaa gtgacagtgc tggaccagca ttttgattgg ccgcaacccc
     5881 tgtcaccaca aatattcccg taccgatgat cgcaccaatt ccaagcatga tcaaatcaga
     5941 cgtttttagt tcttttttca tctcactcgt atgattgaga ttgatctctt tcttacgaaa
     6001 caaatccatt gaagttcctc cccattttag gttggaaaac agcgattaaa ctgttttcat
     6061 ataccctaaa aggataccac ctaaaatgac taaaaggcaa ccaacaataa cataaatgag
     6121 ctcttttttc gtttttcttt cgccaaggat gaaaatccca cctaaagtag agataatgat
     6181 ccccatttgt gaaagtgaga agccaacagc taaaccaagt gatttgactg ttagaagcat
     6241 gcagatgttt cctaagcccc ataataatcc acagatcatg tttttccaca caaactgatc
     6301 gactttaact ttacggaaag caaagaagct tgcgcctaag atcattccga tactttgagg
     6361 taagataatt gacatcgcat ccaaattcgc ccagttgacg atgatggtat atacaccata
     6421 accaatcgta gagaagatca atgctctaaa acccttacca aaatcaagca ttttattatc
     6481 tgtatctatt tttccttcgt catcttgtcg ggcagtcaaa tacgcaccgc ttacaagaag
     6541 agctaaagca ataataccgt atacataatc tttcgtttgt gtccattcat ggaagaaaac
     6601 ggctcccgca atcgtattca aaattaattg aaaaccagta gacaatggta gccctacaga
     6661 aacacccatg tacttcattc catggaactg tccattttgt ccaacactcc aagctaagcc
     6721 ggataaaaat ccgatcaaga aaatccaagg ggtgattaca ggactaacaa cgaaaaagac
     6781 aactaaagaa aacagaaatg caccgatcgt cattcctagt gtttgttgat tggcatcgcc
     6841 accaatttta ccacttacta aaccaatact accccatgcc aacataggga ctaatgctat
     6901 taaaatttcc ataaccaatc tccttttatt tcacatgata agaaacttat cagcgaaaac
     6961 gttaacaaca caagcacttt ttttcgtttc taacgatttt ctaacgtttt tcattataaa
     7021 aatttaagat tgggaaaaat aaagtcattg ttgtcccttt tccttcagtt gattcagccg
     7081 tcaaatggat acctaatttt tcggccagat tttttgctaa ataaagtcct aatcctgttg
     7141 aatgtgtttt gctaagtcga ccatttctcc cagtaaatcc cttatcaaag atccgtttca
     7201 agtcttccgc tgggataccg atccctgaat ctttaactga aaggctgact ccttgcgctt
     7261 ccttttctat gctcacaatg atgtttccgt gatccggcgt atatttgatg gcattactaa
     7321 gcaattggtt gaagataaaa gcgatccatt tagcatcggt caaaacagta tgatcctcac
     7381 caacgatctc gtagcgaata tttttttgga taaaataatt tgcctgactg cgtatcacag
     7441 gctgaatgat tccttttagt ccatattctt ggatgaggta gtcccgcgag aaactgtcga
     7501 gtcttgcata atacaaaact tgttctacgt attcatcgat ttttgataat tcattttcta
     7561 gtaatgtata cttttgatcg tcaatatcga actcgatcga acgaactagc aatttagaag
     7621 cggaaagtgg cactttgatt tcatggaccc atgagtcaat ataatctttt tggtcttgtt
     7681 gattactgat tgctgattgc attacttctt ggtgttcacg gatcagctga ttgatatatt
     7741 cttctaccag tttttcttct tctgtcctcc caccactcaa ataattttgc aaaggggaag
     7801 ttttttgtat gtcggctaat tttccccacc atcttctctt gctcaagtaa ccacccagca
     7861 gataaaaaat cagaaagacg ccctcaatca aagctaaata agcaattgtg ctcaaatcaa
     7921 ctttgatgtt tggcgccagc cacatcacaa agatcgtcaa tgcaataaaa aacagccatc
     7981 ccaagatcaa tgtccactga tctttcaaat atttaaaaaa attcatctta accgctcatc
     8041 ctttcaggga acgatatatc cctgtcctac ttttgtttcg atatatcctt ggatacctgc
     8101 ctgttcgatt tttcggcgta accgattgat gttgactgtc aatgtattgt catctacaaa
     8161 tcgttcgtca tcccacagtg ctcgcagcaa tttttcccga cttaagatct ttccattgtg
     8221 cttcatcaaa ataaataata gacgatattc atttttgctc aaatcaatga tctctccgtt
     8281 gatttccata cttccgttgt caacattcaa cgtaatgcca ttgtggctca tcatttcact
     8341 agataattct gtgtagttgt aagaacggcg aagtaatgca ttgatttttg caatcagtac
     8401 atcgatttgg aaatgttttg tcacaaaatc atctgccccc atgttcatag ccatgatcat
     8461 atccatattc gtattcctgc tggagataaa aatgatcggt actttcgata cttcacgtat
     8521 tttttgacac caataatagc catccaaaac gggaagatta atatctagta atacaagctg
     8581 tggttcttct ctttcaaagt ctgagaacac ttgattgaaa tcggtcgttc caaaggtttc
     8641 aaattgccat ttctcaagtt cctccatgat caattgtcga atgatctctt cgtcttcgac
     8701 taccatgatt tttgccataa aaaatccctc catctttctt cttgctacta caatatcaaa
     8761 gaagcccttg tgaaacaagt tggcttacct ggaaaatgtc attttagcaa acgttaaaaa
     8821 aaactggaaa tatgcgattt aatcgcgttt gtctcttcca agcagcttaa atttattatg
     8881 ttattcttaa taattggtct attatctgtt atttttcttt cgttgttttt gtcttttaaa
     8941 ttttcgataa caccaataac cgaaaagcgt tagcactagc aatataccta gaaggagtat
     9001 aagtaatcga taaagatggt atttttttgt ctgttctatc tttgaagaga cttcttctgt
     9061 aaagggaact cgtcttcctg tcactagtaa tcggtgagtg ttcacggtgt aaggtgtaca
     9121 agtgataagt gtgactagat cctgcttttc tctaattgct aatgtatttg tttttgttgg
     9181 aaggacaaca attttctcaa cgacttcata agcgagcatc cgaccataca cttcaagata
     9241 gaatcggtca tcttgctcca tttcttcaag atccgtaaag agttttttct cagcaatacc
     9301 gctatgggcg gtaatcacgg aatgtgtact ctctcctcca gttggaaaag aggtgccttg
     9361 caggactgtc gctcctctat ctaacaaaag atcattcgtt tcatcaaata aaggcaagct
     9421 aacattgatt ttcggaatat agatggctcc gatcatatgt tgttcgtaat attcttttcc
     9481 tggattttgg acatttctaa ccgcttgctc aaaaggatct ttgacttgtc ccattcctgg
     9541 gattactgat gtatgagcaa gcgtctgatt tttcttttca tgcactgcta attgttgctc
     9601 atgtttttct tctttatcgc gttcaagctg cttttgataa ttctcgatcc ttctttgatc
     9661 caaataattg ctgagagcat ctgctaaaaa tggatatgta aaaactgcag ctccggcaag
     9721 aaacatcact gccataaata attgtaataa aagactgatt tgggtttgag gtctattacc
     9781 tattttcttt ttcattttct tcaccttctt atttaaaaaa actgcagcaa cctttataaa
     9841 accgattgct gcagcttatt tttgttgctt atacttcaga cttcatttta gatttacggt
     9901 accagctgta cgctccaatc atcaaactta ttccgattag taagaatgcc aagagcccgt
     9961 tcccaccagt agcaggaagt gacccgcgtt tcatattcgg tactctttct ggccctggat
    10021 tttctactcc tacgatctga atttcttcat tatagcttcc ataagagatg ataaattcag
    10081 taaaggctgc ctcacctgtt ggaagtacat aaccttctgg cgccatggtc tctttcattt
    10141 gataggttcc ataatctaac ccttgaacct caaagtatcc atttccatct gcgacaaatt
    10201 ctgttgcttg tccgcttgtt gcccactcaa ttctatctgc tcctgattca taaacagcta
    10261 aaggcgactc ccctttataa aatattgcgt attcagaacg gtcttcattc cacaattcga
    10321 atcttgcatc tttaagcaat tcactgcttg atgcatcata tttttcaaac tttcttccac
    10381 ctgttgtgac tttctctggc ggctctactc cttcttcata atcaaattca tcatttcgtc
    10441 cgatttcaat aaccgctgtg ttgttatgga aatcatccgc ttctagttct gtggaaacct
    10501 tcaaattata cgtgatcgtt aaatctcctc cacgtgcatc tgctaatcta cctaaagtat
    10561 ctactttggc tactccattt tcttctgtca aaatcgtcac gacaaacccg ttgcttacta
    10621 cttctactat ataatcttgt ccttttgtta aaatcgtctc gccggctcgc acttctattt
    10681 cctgatcata ataagttaga ccaactgtag gcgtatctgt gattttgtaa tttttgataa
    10741 cagcgcttcc gttttcaagg acagaatcga tgaaataagg aataggtgct gtgatcgtgt
    10801 aagaagccgt ttttcctctt tctagatcaa tataagaaat cgtttcttgt tctccagctt
    10861 cattaataat cgttacttgt ctaaggtcgc tttcctctac attcatctgt ttatctgctt
    10921 ctctgatttc gtttttcgga tacaaatgca catctgtatt gtatgtgtct ttccatgtag
    10981 aaccatccca cacgccatcc accatatcag gcatcatcac tggcatcata acaaccattg
    11041 gagacgcgat tctggaaata tgagctggcg aataagtttc taagaaaaga tagactttat
    11101 ctctagaaga aggtgcttct tccgtcactt ggacggtaga gaataccgct tctccagctt
    11161 cattcgttgt ttgtttgtca atacttgtca gtcgatattt taaaaaccaa ctttctgtgt
    11221 gccgtttttg aatccattca atcgcttcgg cagaattaag accgtcttct cgttccggtg
    11281 ttaaaggatt atctcgaatc aaatcgtaat atacttccgt tacgtcaaac aaatcaaaat
    11341 cgactcctgg aatattttct tcaccaaaat ccgtagaaac caatccgcta ttcggtcgtt
    11401 cttccggaac agatgagaaa ccttttttgt gtaatgtaat cgtcacttca cttggttttt
    11461 gtgcgacttc ttcggctttg actagttttc cgttcccgaa aatccctatt cccgcagtaa
    11521 cgagtaacag tacactcatt cccgtgacaa tcattttttt attcattttt ttctccccgt
    11581 tcttttttta ttaataaaac agcacctaac aataaaagaa ttcctagtaa agaaatcaac
    11641 gttcttgctt cattcgtttg cgggaatacg gaccattttc ctgattgacc tggattgctt
    11701 ggaccactcg gctctttcgg tttgtccgtt gtttctttta cggtctcgtt ataaacacgt
    11761 ggtgttgcgg attcataggc agagactgga actttcccgg attctaattc agccatctcc
    11821 tgtccgttaa ctactacata ttgtggttgg ccttttgcat ccacccaaaa ttcagggatg
    11881 atgacctcga ttctcttact tgcttcatcc atctcgtaac cttccacacc ttttaattct
    11941 tcaaaataat aggtgccaga aggcaagaaa cgttgtcctg tcgtaacaag gccctcttta
    12001 tccgaaatga atttgcttac atttttgtca ttcaaaggat cattgtctgc tggtttgatc
    12061 cattgatttt tcaagtcatt agcaggagcc atatctaaat aatatttccc cccttctatc
    12121 atctgatata gagcaaaaac agcaccttct aatggttttc ctttttcagt tgttcctctt
    12181 tgacgtccat acttgaagaa gtatggatct cttagataac cgacattttt aggatagata
    12241 tgaatctctt gcagttcctc ggttgattct gtcgggtgat aaattggcaa aatagcagca
    12301 attgggatag ctgtctgatc gatatcaaca tccaatccaa cttctggatc aatcgattct
    12361 tcgaaaatca aataaactgc atcccgatct tggtttttct taggtgcttt gatacgtgca
    12421 atcccgtctc ctaatccgcc aaccgtatct ttcccagttg ttaccgaagt ccctatttgg
    12481 acaagccttt cttctgtgat tagctgacgg atattgtttc tgttcgtttc agcgatttgc
    12541 tggacgaatt ctttaggagt catgttttca gcagcttttt tttcatgata ataatcggtc
    12601 atgtcgtaaa tggcaaatgt cgctccattt agtggcaccg tctgtgaaat gatatctgct
    12661 gtttcccctg cttcctcaga gatagccaag ccatcatttt catggtattc aaattgatcg
    12721 ttataatcga tatctcgaat catccgttta tgcaacacga attccacgac atcttcccct
    12781 gattcttctg ccgacatcgc atgtggaaga aggactgctg caagcggtag taaaacaagt
    12841 aattgaagca tcaaacacaa cagctttttc attaccttgc tccctccttt ttagattgtc
    12901 tgacaaacaa accataggct gctcctgcaa gcgccatgaa gaatgcagca agcaggatat
    12961 agaataatcg tccggatcct cctgtagcag gcaacggatt ttcaggatcg ttcccgacac
    13021 taagctgtaa tctgattcga tttgtctcca aaagttcata ttctaactct tgctgtagat
    13081 caggcgtctc tgcttcttcg tttggataat acgaaatctc aacttttagt gtacccgctt
    13141 cttttcctgg aactacccga tcattttcta tgtcaggttc ttctcgataa ctaatttcta
    13201 aaataaagta acctggaagt tttctatagc tgtcaggtga cttcacttcc ttgatccgat
    13261 agaccccttc caacaagtct gaagcattga atagggaaga atcgtttgga tctggtgcca
    13321 agcctccctc gatgatttca tactctcctg cactattttg tttctcaatt gaaaattctg
    13381 cgccttcaag acgtcggcta ttcatactgt caattttcaa aagttccaaa tcgattggcg
    13441 ctagttcatc ttctaaggtt acttgtagta caccatcgat gatttcatag ccttccggca
    13501 agggagcagt ttcgtcttcg atgaccgtat cttcaatgat aaacttacct tcttcattca
    13561 gtgcaaactg gaacacttga tcagatagcc gatacccatt tggtgcgaca gattccgaga
    13621 cctcatattc accaatagac agatgcagag gttggtcgta atttaaatcg acatactttt
    13681 ctactcctac ggcacgagaa tagaagtatc cgatcccagg caaacgtttc gattcttctg
    13741 gttctaacgg attaccagtg tatgctcgat acagcgggaa ggaagaatcc cccactttcc
    13801 tgatatcgaa acctgcacca tctaatgtat ggccttcatg gtctaatttt ctaactcgga
    13861 tctcccgata atcaaacgta ttgtaagcag tgaatgctac ccataaacga tcctcttcat
    13921 ctgttgtata cgtgagatat tgccaatttt cttcacctgt ttgcttgagt tggatagtcc
    13981 ctatagcatt atcgtaaagt cgcaagatcc atgtgtcatt ttcatcaggt tctttgaaac
    14041 cttctgggtt ctggctctca atgattttgt attctgcata gttggtagtt tcttcactat
    14101 ccttataaac aaatgaatct ggctctccga ttggatgaat aactttctca ccatccacga
    14161 ttacatattt cccgtcttca tctactgaat agaacgtgag tctccctttc tcttcctcta
    14221 cttcgttcac accagaaagc cctttcgcta ctggatatcc gatttccagt ccgttaccat
    14281 ctgtttcata aagttcaaac tctgcgccat tgattggcat attactgcct tcaattagtt
    14341 tattgatatt catgtggatt ggcttgaatt catttcgcat ttccagtttg acatacaact
    14401 catcatactc tgtgccttct atgttaggct cttgtacaac agaaagcttg tgatattctc
    14461 cttcataata ggaattcgga tctttacctt ccggtccgcc gactctcgaa ccagatgtat
    14521 attcatctac aagctcgtag tgacctaaca tctcatcttc gttggctgca tattttttga
    14581 tttggaattg atacgtctca tctactaact gataacgtgg tggtccagcg atctctctga
    14641 tccaaaatat gtcgctgact gctccagttt catctcctaa aatcggcatc ttcgtttcaa
    14701 agatcgctat tccgtccgtg gtagttttac ttgtttcgat tggttcggct tccccactct
    14761 ctggattttg gtcatataat gcaaattcga ctccagccaa cggctcgcca ttttgaccct
    14821 tcttgctgac ttggaaatta cggcctttat gttcgtaacg aatggaagaa gatgggaaat
    14881 acatccgtcc agtagtgata tcattctcat aatcctccag gttctgaata taggccatat
    14941 tgttcactgt gcaaaaattt ccgtcttgag ctcccgcatc aagttttact tggtacttga
    15001 tcctgaccat ttccccttca cctaaagaaa tcggagaaaa tttataagct tggttttccc
    15061 gtccgacata cgttgtcgga gtcgccacta cttctggtgg ggcttgtcca tgacgttctt
    15121 catcccatat ttctggctct tggtacttca tgccgttttc tcctggatct agatggtaaa
    15181 actgaatatc tgccgatctg ccagctccaa atggtgagac attaaaacca gtttcatcat
    15241 aaagtgtggc ttgctggatc gttctcttga aggtagaaga gatatcctcc aaggctgtaa
    15301 caatgtcatt cttgttgttc gcattgtaat atcgcggtgt tacgccatct tcttctaaat
    15361 cagcaatatt tttcaataca tttcgtccaa tagcatctag tcgttgtctc ccgctaacgt
    15421 ttccatcgat tcctaaacca atggagaaga tacttggcgg gttctcttga tacttgtttt
    15481 tcagccaatg atgataagca actgtgttga tcgcagatga cttttcttgc gaagtaggac
    15541 tgccattcgt attgttatta gaagaaccat caccaaccca gtgagtcgct ttattatctc
    15601 cttcttttac ctctccataa cgatagtgta catttacttc atcttctgga cggttaaagt
    15661 cagatgcata ggtaactgga tgagtaaacc ctgcattaga agtattccca gaaaactcct
    15721 caaaatttct gaacaaaacg tcattatctt cagcaatctt atcttccatc acagtccagt
    15781 tgctccaagc tccattagtg ctcgtacgat aacttgactg aatcggtgca taactgaagg
    15841 tcggtgtccc atctcccaca acgattagga ttttttctgg atttctattt tctccgccat
    15901 tgtctttata caatgtttca tagccatttt taagccctag tgtaagcgga gttcctccga
    15961 ttggtgtacg atttaatgct gtattctcac ttcccgtcaa acgattgata tcatttgtca
    16021 atggaaagat gtccgaactg atttgctcat ggttattgat agctgtacta ttatggtaaa
    16081 aattcaccat accgattcga atatccagac tcgggtcaga gagcagatct ttactcattt
    16141 cgtttactgc ctcaatcaac gcagcatttt tagtttgact attggtccca gcggttaatt
    16201 cactcatact cgctgactta tctagaacaa aaacgatatc gattggactg atttcctttt
    16261 tctcgccccc gatgacatct agatagacat tgtaaagggt tgttgcatct tcaggatgta
    16321 gatcagatgc tgtgctgctt ggcagaacag gttctaccca ttttttgatg tacgccccgt
    16381 tataatccaa gtaactgttt tcaaaattcc gttcatttcc ccaaagctga acggtttgag
    16441 ctggaacgtt tctatcttct tcgcctgcag ttgatccatt attagtagca tagtcgtaat
    16501 ttcgccagtt cgctcccccg tttgtataat cactatcatc gatcaagttc tctccgtcat
    16561 atggcgagcc tttgtctcct tgatagcgtt cattgctttg tgtccaattt cctaacactt
    16621 ttgtatagtc attcgttgta aacttcatct attctttcat caaccaccac ttgaagaggt
    16681 agttaccctt cgttcacatc ggggatcttt tctgtttcaa aaatcaatgt gccggtttct
    16741 gcttcttttg aataaacttt tccgacatac cattcttgac catcgatcgt ctcgactttt
    16801 aggtcttctc catcttcatc ataggaggat agacctgttc cacgaacgtt ttttacggta
    16861 ccaatcccgg cggctgccgc atcgattgct aaacgtattc taccttcatt ggcactttcc
    16921 tttttttcaa aagccagatg ccattcaagg gtttcttctt tttcaatata cttcccttca
    16981 atttttgcat agtcctcatc taagaatacc tcatcctcct ttgtttctgt tgactcagca
    17041 gctacatcca gggtcgtcat atgatagact gactggaaat atggtccaaa tagcaaactg
    17101 acagttacta actgcaaaag cttcaatttt aaccctttca catccttata ctcctttcaa
    17161 aaatatttgt cttctttcac aacaatcaac aaaacaatgc ccatctaatt ataatttaat
    17221 ttaattttaa ttttaaatca aatgatatcc ttttt
//
LOCUS       DAZABD010000055        16615 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000055 DAZABD010000000
VERSION     DAZABD010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16615
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.055"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..525
                     /gene="scm"
                     /locus_tag="ACHALG_002029"
     CDS             <1..525
                     /gene="scm"
                     /locus_tag="ACHALG_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFN2587404.1"
                     /translation="TSASSDSTDTTSTTSDSTDTSASSDSTDTSTTTSDSTDTSASSD
                     STDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSD
                     TRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVI
                     GLRITFMSYKKSKR"
     gene            complement(680..1666)
                     /locus_tag="ACHALG_002030"
     CDS             complement(680..1666)
                     /locus_tag="ACHALG_002030"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFN2587405.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(1689..2255)
                     /locus_tag="ACHALG_002031"
     CDS             complement(1689..2255)
                     /locus_tag="ACHALG_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HFN2587406.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(2269..2685)
                     /locus_tag="ACHALG_002032"
     CDS             complement(2269..2685)
                     /locus_tag="ACHALG_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFN2587407.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(3142..5076)
                     /locus_tag="ACHALG_002033"
     CDS             complement(3142..5076)
                     /locus_tag="ACHALG_002033"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFN2587408.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5378..6313
                     /gene="pplA"
                     /locus_tag="ACHALG_002034"
     CDS             5378..6313
                     /gene="pplA"
                     /locus_tag="ACHALG_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular electron transfer flavoprotein
                     PplA"
                     /protein_id="HFN2587409.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            6403..7506
                     /locus_tag="ACHALG_002035"
     CDS             6403..7506
                     /locus_tag="ACHALG_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFN2587410.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            7519..8472
                     /gene="menA"
                     /locus_tag="ACHALG_002036"
     CDS             7519..8472
                     /gene="menA"
                     /locus_tag="ACHALG_002036"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFN2587411.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(9407..10753)
                     /gene="gor"
                     /locus_tag="ACHALG_002037"
     CDS             complement(9407..10753)
                     /gene="gor"
                     /locus_tag="ACHALG_002037"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFN2587412.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(10887..12140)
                     /locus_tag="ACHALG_002038"
     CDS             complement(10887..12140)
                     /locus_tag="ACHALG_002038"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFN2587413.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(12146..13069)
                     /locus_tag="ACHALG_002039"
     CDS             complement(12146..13069)
                     /locus_tag="ACHALG_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFN2587414.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(13290..15782)
                     /locus_tag="ACHALG_002040"
     CDS             complement(13290..15782)
                     /locus_tag="ACHALG_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HFN2587415.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(15789..16256)
                     /locus_tag="ACHALG_002041"
     CDS             complement(15789..16256)
                     /locus_tag="ACHALG_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HFN2587416.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(16392..16465)
                     /locus_tag="ACHALG_002042"
     tRNA            complement(16392..16465)
                     /locus_tag="ACHALG_002042"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16429..16431),aa:Arg,seq:acg)
     gene            complement(16523..16596)
                     /locus_tag="ACHALG_002043"
     tRNA            complement(16523..16596)
                     /locus_tag="ACHALG_002043"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16560..16562),aa:Pro,seq:tgg)
BASE COUNT         4907 a         3475 c         2958 g         5275 t
ORIGIN      
        1 acatcagctt caagtgacag tacagatact acctctacca caagtgatag cacggacacg
       61 tcagcttcaa gcgacagtac ggatacatct accacaacaa gtgatagcac ggacacgtca
      121 gcttcaagtg acagtacaga tactacctct accacaagtg atagctcaga ttcatctacc
      181 actacaagtg atagcacgga cacgtcagct tcaagtgaca gtacagatac atcctctacc
      241 acaagtgata gctcagattc atcaacttca agcgatagta cagacacgtc gtctaccaca
      301 agcgatacac gtcatgcaag tgtctccttc tcaaaaacaa gttcggataa caggacgaac
      361 tttaacttaa ctaataaaac aagcaatgac aatggaaaca aatcaaaaag agcacttcct
      421 aaaactggaa gtcaatcgaa taattggata acactcgcag gcgttatatt acttgtaatc
      481 ggtttaagaa tcacttttat gagttataag aaatcgaaaa ggtaaaaaaa tattcaagat
      541 aaaaaagagc aacgtcgcta acggacgttg ctctttattt ttgcttttta tcataaatac
      601 aagaactaca attcaatttg tcaaaaaaca cgtagatttc cttctgagaa atttatttac
      661 gtatcgaacc tttccctatt cagtctttgc gatccaaaat cagttgggta agggaaaaga
      721 gattctcttt tgttcgaaga gaggtggcag gtaatttctt tatttccttc aaagcctttt
      781 ctgtgtaaga ggccgctaac ttcctagctt cttccacgcc tcctgtatgt tcaacgattt
      841 tgcgtacggc gtcgacatct tcttctgtca ttgccgcttt cttctctaaa taaggtagta
      901 gctttggttt gtttttttgc aaagagtaga tcaacggtaa agaatagacc ccttgacgca
      961 tgtcttctag tacaggtttg ccgatttcct ggctctcttt tgtataatct aaaatatcat
     1021 cgataatctg aaaggccatc ccgatagaaa gtccgatatc tcctgctttt tttgcgaagc
     1081 gctcgcttgt tccactttca aaagcgccaa cagaacaact taattggaac agttcagctg
     1141 tcttacctga aatattttcc aaatattctt ttacagaaag ttcataatta tagcgatcat
     1201 ccatctgccc taattcgcca ttcaatactt tttccatact gcgagaattc aattggagac
     1261 ttttcaagga agtggagtaa tcagacaata gtttaaaaca gcagacaaat aaatagtctc
     1321 ctgcatagac agaaacgaca ttaccaaact gactacggat attaggctga ccacgtcgca
     1381 gatccgcttc atcaacgata tcatcatgga tcagtgtggc cgtatggagc atttcgatag
     1441 aagctgccaa agcgatggct ttgtctcgat cttgctctgg tccaaattgt gagaacaata
     1501 gctgataggc aggacgcaac agctttccgc cggcatggat cgtttctttg atggcttgtt
     1561 cgacaggttt gtttttcagg cggacagttt cttccatccg ttttagtgtt tctgtcaatt
     1621 ctttacttaa agcaggatag ttttcccaca ttggatgtag cttcataaaa aaactccttg
     1681 tgtgacgatt attttttggt aaacattggg ctccattttt gtttggttcc ctgctcataa
     1741 tctttttgga atcgttgaag tgtctgcaca tgtcgtaaaa gataattcgc tgcgatcccg
     1801 attgcaatcc ctgataaaat ccctaaaact gaaaggactg gcaagtacaa catgactgtc
     1861 catgattgtg caatgaaaga agccatcaaa agctgtccga cattatgcat gaaaccgccg
     1921 gccgcactaa ttccgatgat actgacacgt tttggtccta attgcttgac caataacatt
     1981 ccaaaataac taagcaaagc accactcata ctataaagga atgtggaaat cgttccgcct
     2041 agcaaagtag tcaggaatag tcgtagccaa agtagtaaga agctgtcttg ttttttcatg
     2101 gtaaaaatgg caacgatcgt gattagattg gctaagccta gttttgcacc aggcgcaaaa
     2161 gaaaaaggat aagggatcat attttccaat aaaccaatga tcactccttg tgcaacaagt
     2221 agcgaaatat aaatcatttt ttgtaattta ctcatggtgt gtgaaggtct aataaatcag
     2281 actgtcttcg tcacctccat ctgttgaacg gacttcgatc actaatttat gtggcaaaca
     2341 gacgatggtt tcgccatttt tcgttgccca gcctcgacgt acgcagatct ggtcatcgca
     2401 atctgcttct tttatccgta ctcgatcgcc atctatctcg atcaagttat aatctccgtg
     2461 ttcatctttg taagtatatg tgttgctctc ttctccagcc atcaaaggga atgttttgat
     2521 ttctgtgcca tccacacgca gtaccgcttc tttttctgct gttgcggtgt tttgctgaag
     2581 accaaaaatc atgactggta aaaaagaaag aacagtcaaa agcaaaataa taaaaacatc
     2641 ccaaggtttg agcagacttt tttcagcaaa ttctttaagg ctcatttttg ttctcctttt
     2701 tccagattaa ctcacccaat tttagcataa aaacaagagg ttgtgacacc tgtgtcttgc
     2761 tttgagtaat aagaacgaag aatagaaaac agctttccat gttttctaat tctttgggct
     2821 tattaccgag aagcagcagg tggaacaccg tttataacaa gaggttgtga cagaagtggg
     2881 caacttcaag aaataagaag gaatttacga aaattacttt tcaagttttt gtgaaatttc
     2941 ggcttatttt tcgaagaagc tacttctgga acaccgttta taacaagagg ttgtaacaga
     3001 ggttgtgaca cctgtgtctt gctcatggag gcttatctcg ggcatcttat tcctttaatt
     3061 ttacataaaa aggaggctgt gacggtttgt ctcagcccct ttctattgaa atcttgcatt
     3121 caaataacgt tctttatcta tttatttctt gccgtactgg gaacttggtc taccgtacca
     3181 ccaattacct agtgtacgtt gtagccatgg aattacccag cgatccaacc caatcgcacg
     3241 tccggaaccg ttcatcaatg cgaaagcaac aaaaatgaac caaatgttta cccagtaaaa
     3301 catgcctgat aagcagaacg tgatcaccaa tacaatcgtt gtcgcactgc atagccatgt
     3361 gaataatcca gcaatcaatg ctaatgccaa ggcaatttca aagaatacca tgaatttttg
     3421 gaagaacatt gctacatcat tgtttggcat gatcacttgc atgatctttg tgaaccagtc
     3481 tggtgacttc tcaatcacag ccattggttc ttcaccgtat gcataactca aaccaaaatg
     3541 tgcagcttga gtcgctgctt ctccggcact tgcccctgct ccgctggcag ctgtggttgc
     3601 atcaccagca gcagaagcac cactggcaac atctgttaca ttgtattccc atgtccaagg
     3661 gaacacactg tttccagtga accatgatcc ttcaccaaac caagtacttg gtttgaacac
     3721 ttcaccattc ccaatgattt ttttggaagc ttcaacgaac caaactaaac catagaaaat
     3781 acgaagtggc acactccata agacattgcc gtaacgagaa gaatgtcctc gagtcacgtt
     3841 gcgatcatct ttgatatgga aaaactcatg cattaaatat tggaacatat aataaccaga
     3901 acgaatatca aagaagtatt tcaaattcac gatatgtttc attacgatag ctaaaaatcc
     3961 gcttaaatgg atcttatcaa ataggttagc taccccccat ttggcaccaa tagaaaccat
     4021 aaatccttga tagtttcctt taaatttatg cttttctccg cctttgatcg aagccacgat
     4081 attcgctgcg gctgtgtgtc ctgtttgttc agcagcttga acgatctgcg gtgtcggtgt
     4141 tcctgggaat tcttcataaa agactaaatc accaatgata tagatgtttt tatcttcgta
     4201 gccttttgcc tgcatatatt cattggcgat caaccgctgt ccgcgtgctg attctaatcc
     4261 aaagtcagca gcgtcagaag ttcctttgac accagctgtc caaatcagtg tgtgagtcgg
     4321 aatgttgcgt ccgtctttca atttgatatg atcagcagcc acttctacga ttggtgcatt
     4381 caataataat tccacgttgt gcttacgcaa atagcgttca gcttttgcag catcatttcg
     4441 atccaacata ttcaagatcg tcggcattgc ctctacgacc atcagtgtga tttcgtttgg
     4501 atctaattta taatctttgg ctaggcgatt tttccagtcg atcagttccc caatcatttc
     4561 aataccggta aatccagaac cacagacaac gaaagtcagc atggctttac gcagttcatc
     4621 atctggttcc aatgcagctt ttgctactgt cttctcaata tgttcccgaa tccttacaga
     4681 atcttcaaat gaccaaaggg tgaatccgtg tgctttgaca ccaggtgtac caaagtcatt
     4741 cggctctccg cccattccaa ggatcagctg atcaaaagga taggaaccta atttggtctt
     4801 gacgattttt cgttctttat cgataccgat tacagtgtct gtgacaagtt gtacattctt
     4861 ttgacgagca aacaaatgct gcagatcgta ttggattgct tctggttcta cacgtccgcc
     4921 agctacttca tgcaactcgg tcatcatagt atggtacgaa tgacgatcga tcagagtaat
     4981 tgctacatct tcttttttca gtttctttgc caagtatttt gtagctgaaa caccggcata
     5041 tcctgctcct acaacgacaa tattttgctt tgccattgat catccgctcc ttatagacta
     5101 tctatataat gaatatatga taattctttt agtttgatac ttattacaca atctccatat
     5161 attatataga tgttctgttc ttttgtctaa ctccttttgg atttttttca aattttaaaa
     5221 taaagcgttt tttcacgaaa tttgctgaaa gaaaacgttt gaaatattac atgggttatt
     5281 tatcaaaaat agttgactta taggctaaaa agattagaat gtataatgaa tcacaaagaa
     5341 aaattgtgga ctagaaatgg aatggtgggg tattaaaatg aagaaaaaac gttttgtaac
     5401 tggatttgcc gtattagcat tttcagctct agtgttaggc gcttgcggcg cagatgacaa
     5461 cggaagctca aactcttctt ctgaatcatc tacagcacaa tcttcgactg caaaaagcac
     5521 aacagaatca tctgcaaaag ttgtagctgg cggagatttg caagacggca catataaatt
     5581 agaagaaaaa aattattcaa atggttaccg tgcagtcttt gaaatggttg taaaagacgg
     5641 caaaatcact gaatcaaaat atgataacgt caatgaaaac ggcgaatcaa aaacaaaaga
     5701 tgcagaatac aacaaaaaca tggaagcaaa atctggcaca aacccagaaa aatacattcc
     5761 tgagttgaat gaccaattct taaaagcaca aagcgcaagc ggtgtggaag tcgtaactgg
     5821 agctactcac tcatctgaaa gcttccaaaa ctacgcacaa caattgatcc aagctgctca
     5881 agctggtaac acagatacaa tcgaaatcga caatggtgct gatttgaaag acggcactta
     5941 caagcttgaa gaaaagaact actctaacgg ctaccgtgtg caattcgaaa tgacagttgc
     6001 tggcggcaaa gtaactgaat caaactttga ttatatcgac aaagacggta aatcaaaaca
     6061 agatgataca gaatataatg aaaatatgaa agcaaaaagc ggtacagaac caaaaactta
     6121 catccctact ctgaacgacg aattcgtcaa agcaatgggc gaagaagatg gctcacctgc
     6181 taatgtagaa gttgttacag gtgctacaca cagctcacat tcattcatca tgtacgcaca
     6241 acaattagtg aatgcagctg aaaaaggcga cactcaaaca atcgaagtag ataatatcgt
     6301 aacagaaaaa taatcacata atcatactaa taaaagttgg agggtgggac gatagccaga
     6361 ttaggcagtc gtctcacctt tcaaaatgaa gagaggaaaa agatgcggaa aaaactacta
     6421 ttttccttaa tcacactggg catctctttt atggtgctaa ctggctgcag caactcagca
     6481 aataaaacaa ccactgaaag ttcagcagct accaagatat tgaaagatcc atattctgat
     6541 gaacagttct tgttagggac ttacgttcgt atccgtgtgt atgatgaagg caaagaatca
     6601 gcattgaaac ctgccttcga tcgagtaaaa gaacttggtg ataaaatcac gatcaatcaa
     6661 aaaggttctg agatcgatga agtgaatgag caagctggga tcaaaccagt aaaagtttct
     6721 gatgacgtct acacgttagt caagcgggcc tacgaatata gccaagattc tcaaggcgga
     6781 ttcgatatgg cgattggtgc aatcacacaa ttatggcgaa tcggttttga tgacgccaga
     6841 aaaccttccc aagaagaaat cgatcaggca ttgaaattag tggattatca taagatcgaa
     6901 ctaaatgaca aagaaaaaac tgtttatcta aaagaaaaag gaatgatcat cgatttaggt
     6961 gcaatcgcca aaggttacat tacggacgaa gtagtgaaag tcttgaaaaa acaaggagtg
     7021 accactgcca tcgttgactt gggcggtaat gtctatgtct taggccacag cccaagaggc
     7081 gaaaaccaag actggacagt cggcatccaa gatccgaaca tggctcgcgg atctgtttta
     7141 ggttctatta aagaacggaa caaaacatta gttacctctg gaatttacga gcgttattta
     7201 gaagtagatg gcaaaaaata ccatcatttg tttgatccaa aaacgggtta tccgttcgac
     7261 aatgatatcg caagtgtcac gatcataaca gataaatcaa ttgatggtga cgggttgtct
     7321 acagcagtct tttcaatggg tgtgaaaaaa ggactggaat acgtcgaaag cgaattgaat
     7381 aacgggacgg aagcgatctt cgtgacaaaa gacgataaag tatatgtgac cgatccaatc
     7441 aaagacacct tcaaattatc ggaggactcc cattacacga tgggtgaccg ttctgaacta
     7501 aaataaggag tacgaaccat gacactaaaa atctttttag aagtagttga aatacgaaca
     7561 aaggtggcta gtgtatttcc ttttgcggta ggggtattat tctctatcgc tttttttcat
     7621 gagatacatt ggctaaacac ttttttattt tttattggaa tgctagtttt tgatttagcg
     7681 actaccgcta tcaataatta catggattat gaaaaggctc attctgaagt atataagtat
     7741 gaagaaaatg tcattggccg cagaaatcta tctcctgtat tgattcgaaa catgatttta
     7801 gctatgattg catttgtttt tgttatcggc gttattctaa cgatcaatac gggttggctt
     7861 ttacttttga tgggatttat ctgttgtttt attggagtat tctatacatt cggaccgatt
     7921 cctttatctc gaatgccttt aggggaaatt ttcagtgggg tgactatggg ccttggaatt
     7981 tttgccatgg tcgtttatgt caatacattc ggttcagatg tctatgatct gacagtcaac
     8041 ttttcatctg gacaatttga tttggtagga aatctttggg ctatagcagc aatcgtatta
     8101 gcttccttac ccttggtgtt tacgattgcc aatatcatgt tggctaataa tttgcgggat
     8161 cttgaccgtg atattgaaaa tcatcgctat acactagttt tttatatcgg acgtcctgcg
     8221 ggaacagtct tattccagct attgatgtat gcttgctatt tcgtgatttt aatggggttg
     8281 ttgagcggta tctatgaatg gccgatcttg gtcaccttct tgaccttgcc aattgtgaag
     8341 aagcatttgg atcaattcaa gcaaacgtta ccccagccta tcagtttcag ctattccatc
     8401 aaaaatatgg tcttattcaa tgccagttat gtattggggc tattactaac aatcattatg
     8461 caagccattt aacagcatga agaggttgtg aaaaaactgc cctgcatcaa gtaataagaa
     8521 cagccaaata aaaatagctc cttatatttt tcgtttggtt gggcttatta ccgcagatgc
     8581 aagcttttga acaccgttta tcaggaggtt gtgaaaaagt cgcttagttt cgagtattaa
     8641 gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa atttggctta ataccgagaa
     8701 actggctttt gaacaccgtt tatcagaggt tgtgaaaaag tcgcttagtt tcgagtatta
     8761 agaaaaattt tgtgaaaatg gctttccaca tttttgcata aatttggctt aataccgaga
     8821 aactggcttt tgaacaccgt ttatcagagg ttgtaaaatc agtgttttgt cctgagtatt
     8881 agagaaacaa atggaaaaat agttcctcat attttgccgt tttttgagct aataccgaag
     8941 gacagctgat tgaacactat ttgttcggaa aaagtgacta actatgacac aatttttttc
     9001 acagtccctt ttttcacttg atttgattcg taccagcgaa cttgacgaga actttcttgg
     9061 tttggtaact atatggtatt cttttaacat cctcgtttgc ttcttctaat ttatttatac
     9121 aaagtcacac ttttttaaac atctgttctt cgatacattt ctatttcttt taaacatcga
     9181 taccaatatt ttgaacttaa gagtgcatac caaatggatt catttactct tcgtcagtta
     9241 cgaagataat caaaatctcc aacttcccta cttatttcgt tttgagaatt atcgaagcaa
     9301 tcttacataa ataactaaaa aacaaaaaac cagaaaatca gtatactgat tttctggttt
     9361 tacatggttt gttcaataaa aagctgttgg aactgttaat attaaattat cgcatcgtta
     9421 cgaattcttc cgctcctgtt ggatggatgg ctactgtatt gtcaaagtct tctttagttg
     9481 cacccatctt gatagccacg gcaaaacctt gcagcatctc atcgacacca acaccaatag
     9541 catgtaagcc gacgattctt tcttcttttc cgacacagat caatttcatt tcgcattttt
     9601 ggcgataatc atttaatgca aagtacattg gggtaaagcg tgaacgataa actttcaatt
     9661 cattttcgcc atatttttcc aatgcttctt cttctgtcaa accaattgtt gcaattggtg
     9721 gatgcgtaaa gacgactgtc ggaacaagtt tatagtcaag ataagaatct ttctttccat
     9781 taaataaacg ttctgataaa cgtctaccag ctgcgattgc aacaggagtc agatctaatt
     9841 taccgatcac atcaccaaca gcaaaaatat gttcatccgt tgtattttgg tatttatcta
     9901 ctttgataaa cccttttttg tccaattcca catttgtatt ttccagacct aatttttcgg
     9961 tattcggtac acgtccaccg gcaaaaagta cacaatcacc agttaaggtt tcaccatttt
    10021 caaatagcac cgtatattcg tcattgtttt tagtaatttc tttagcaata tgatgtgcgt
    10081 aagtttttat tccagcttct tggtacattt ggatcaaatt gtccgaaagc attttatcaa
    10141 acgttctaag tggtcgttct tttcgatatg cccacatgac ttcactacct aaaccacgaa
    10201 atacacctga aagttcagcc gcaatgtatc cagcacctaa aacgattgct ctcttaggta
    10261 actctgttaa ttcaaaaaat ccatttgaat caattgcgta ttctccacca ggaatggtca
    10321 ttttacttgc acggcctcca gtagcgatca agattttagg agcacgatag gtttcaccgt
    10381 ttacttcgat cgtttgacta tcgataaaag tagcgtaacc ttttatagcc tcgacatgat
    10441 tgctatctaa tcctctttga taagcaccgt gtagaaaatc aatatatttt tctcgtcttt
    10501 ctactagacc ggcgaaatca acatttttta aatctgcttg gataccgtaa cttgaggcat
    10561 ctcgttcaat tgtttccaaa atcgtacttg cttgccacat aactttttta ggtacacaac
    10621 cgacgttgac acatgttccg cccaattcat tcgcttcaat cagcaaaact tttgcgccat
    10681 gcatccctgc tctgtttgct gaagcaatac cgccagagcc gccaccaatt acgatgtaat
    10741 catatatttg cattttgttc ctccttattg aagagatcaa tcaaaacctt tatttaacga
    10801 tcctttttct acagaaaaca agtgtaacac ttttcttgaa ttttgtatgc gaagatgctt
    10861 aaaacaggga tatatcagct ttttccttat ttacgttttc ggatcatatc tccaggtttc
    10921 accggctgtg cgactggcat agttaaaatc atcattggat ttggtgctcg gtcaatggat
    10981 tcgccttctt cattatgcat aacttcaacg gtttgactga aatgactgaa tcctggacca
    11041 taaaattcga tttcatctcc tacagaaaac aagtttctct gacgtatcgt agcaatctgt
    11101 gttgcctcat cataagataa tacttcaccg acaaatttat atactgggat tttacgacgt
    11161 tcaccgaata attgttcgtt ttcagttggt gtattgtagt aaaaacctgt agctaattct
    11221 cgttgtgcta ctttccataa ctcgtcgatc cattcttgct tgcagacata gttttcagga
    11281 tcttccatat aactgtctac agccgcttta tagacatttg acaccgtaga tacataatgg
    11341 atcgatttca ttcttccttc gatcttgaag ctgtctacgc cgttttcaat caattctgga
    11401 atatgttcga tcattgacat atccactgca ctcatcgaga attcttcttc cacttcgcct
    11461 gtatcagtta aactgtttcg ttcttgacca aaaggcatat cgaacaatcc atacttccag
    11521 cgacaagatt gagaacatcc gccacggttt gcatcccgca tcgacatatg gttggacaat
    11581 gtgcatcgac cagaataaga gatacacatg gcaccatgga taaatgcttc gatttcggta
    11641 tccgtatttt tacgaatctc tgccacttcc tccattgaaa cttcacgagc taaaacgaca
    11701 cgttccaacc cttcttcttt ccagaattcc aatgtctcaa aattagttgc tgaagcttga
    11761 gtggataaat ggatcggcag acctggggct tctgtcgcac aaatttcgat caaagctgga
    11821 tcagaaacaa tcactgcaga aataccgaca tcacgcaaac tgcggaaaaa ttcccctgct
    11881 ccatcttcat ttccttcatg ggtgaccata ttagcagcaa cgtatacctt cgcatcatgt
    11941 tcttttgcaa aagcaactcc ttcagccatt tcttcataag taaaattacc tgcacggctt
    12001 cttaaaccat atgcattccc gccaatatat actgcatctg ctccataatg gatcgcggtt
    12061 tttaactttt ccagtgttcc tgctggcgct aggatttctg gtcgtttcaa ttttcttttt
    12121 actgtcattt ctttcatcat actccttatt tgatagcaga aggatcaata taaaagaatc
    12181 ctgtatctaa ttcacggttt tcaggatgca atttactgat ttcgctagtc attttttccg
    12241 ctttttcttg atcccagcta ccttctaaaa tttgtttttt tgcttcggcg aatagtcgag
    12301 tgatgtttac gaatttttcg cctggagtaa ataatccgtc caatttccat gtacagtaat
    12361 gatgctgata aagttcattc aattcattcg agagattcag atcattgctg gcaaaaatat
    12421 gtgtgccatg actatcttca taaatagaat aatgtgtctc ttctttttta ggttccgaga
    12481 taaaaagacc tctttcacga tctttttgtt cgtcttgttt cgtatagtta tagtaattct
    12541 gtagcagtgg acgtttggat tgatggatgc atgtagcacc atacacaagg gtttctacag
    12601 gaatatcgag tttttcttcc atcgccttca tctcttcaaa aggaacttca cgagcaagta
    12661 ctgctccaac tgcgcctttt ttcgcccaga aattgatttg acgtgcgctc gttactaatg
    12721 tttcagcatc ataaatatac ggtatacgca attcaggatt ttttttcatc gtataaataa
    12781 tacctgtatc accaactgtg atcacatcta cgtttatttc ttttaaaaaa gccaaatatt
    12841 caggtacatt tttcattttt tcaggatgaa tgatcccatt tacagcaata ttcgcttttt
    12901 taccatactt gtgtgctaat tctaccaatt gtcgctgttc ttctcgtgta aatgaagcag
    12961 gcagacgcaa tccaaattgc tcttcaccga aatatagtgt atctgcacct gcagcaagca
    13021 actgttctgc ttgttcaata gtttctactg tggttatgag ttcgatcata attttctcct
    13081 cctacaactt cataatagta gcatagctct aaaaactttc caatacttct tctatttaaa
    13141 gtcggatttt tgaaaattat ttcacaaaaa atcaaaataa agttcgatat attctgtcca
    13201 aatataaaaa gagattgtga caaaaatctt gtcacaacct cttaatcaag tcttactatt
    13261 ataaaaattc agctacacag gccaagtaac tatagggaac ctactgtttc ctttttaggt
    13321 ccgcgaacat tcaatgtgat cttacctttt gaagcaccta ctgttacgtg gtcacctaaa
    13381 cgaatttctc cgccaagcaa agcctcgctc aatcgatctt caatttcttt ttgaagtgcg
    13441 cgacggattg gacgtgcacc atattcagga tcaaatccag ctttcccgat cacatcgatt
    13501 gcagatggtg tgattttcac ttgaatatct tgttctttca atcgtttgat aatcgctttg
    13561 ctcatgattt ggacgattgt atggatttca tctttactca atgaatggaa gacgacagtc
    13621 tcatcgattc gattcaagaa ttcaggtcgg aaagctttct ttaactcttc taagatacgt
    13681 ttttgcatgg ctttgtgatc tttagtaaca tcttggacat tgaatccgac atttttttct
    13741 tctcgaattt gtgtcgctcc gatatttgaa gtcataatca tgattgtatt tctaaagtcc
    13801 acttttctac ctttagaatc tgttaaatga ccatcatcca atacttgcag aagtaaattg
    13861 aaaacgtctg gatgtgcttt ctccacttca tctaagagaa tcactgaata tggtttttga
    13921 cgaatttttt ctgtcaactg tccaccttca tcatacccta cgtatcctgg aggagacccg
    13981 attaatcggc ttgtactgta tttttccata aattctgaca tatcaacacg gatcagggca
    14041 tcttcacttc caaacatgac ttctgaaagt gctttggcca attctgtttt tccgacacca
    14101 gttggtccaa ggaacatgaa tgaaccaatt ggacgatcag gatctttcaa tccgcttcgt
    14161 gcacggcgga tcgcacgtga aactgctttg acagcctcat cttgaccaac gacacgttca
    14221 tgcagaagtc cttccagttc taacaagcgt tcgctttctt ttttctccaa ctgctgtaga
    14281 gggacaccag tccactccga tacaactgta gcaacatctt cttctgttac acgatcagaa
    14341 taaccttgct cctgttttgc ttcaattgca gaaacttttt gcagcttttt acgtagtgct
    14401 ttttcttgtc tgcgtaattg agcagcattt tcaaagtctt gtttttggat cgctgcttcc
    14461 ttttcttgta ctagttcatc gatttctagt ttgatcacag ttgattttgt taagtgatcg
    14521 gtttgatcca aacggacttt cgctgctgat tcatcgatca aatcaattgc tttatcgggt
    14581 agttgacgag aagtgatata acgaacagaa agattgactg ctgctcgtac agcttcatct
    14641 gtgatctcca ctccatggtg ctcttcatat cttgtacgta atccttgaag aatcacttct
    14701 gcttcttcag gagttggctc atctacttga atgcgagcaa aacgtcgctc taatgcagaa
    14761 tctttttcga tatatttttg atattcatct agggtcgttg ctccgatcgt ttgtaattct
    14821 cctcgagcta acgccggttt caagatattc gaagcatcaa ttgctccttc tgctccacct
    14881 gcaccaatta aggtatgcaa ttcatcaatg aataagatta cttgaccatc attatagatt
    14941 tcgtcgatga cctttttcat tcgatcttca aattcaccgc gatatttcgt accagcaacc
    15001 aaagatccca tatcaagcat catcaggcgc ttttcttgca tgtcttctgg cacttcacct
    15061 aaaatgattt tttgagctaa cccttcagca atggctgttt tgcctacacc aggttcaccc
    15121 accagtacag ggttattctt tgtccgtcgg ctaagaatct ggataagccg tttcacttct
    15181 ctcgaacgac caacaactgg gtccagcttg ttttcttttg ctaatttcgt caaatcacgt
    15241 gctaatgaat ctagcgtagg tgttccttgc ggtgtttgcc gtttatcttg tacatttaca
    15301 tttctacgac gattcattcc ttttccagca gaatcaccag ctagccccat tttttgtttc
    15361 agaacttgac gggcttttga aaggctgata cctaagttga tcatgattcg agtagccatg
    15421 atactttcat cttttaataa gctcatcaaa atatgttctg ttccaacttt ttgagaacca
    15481 gttttcttca cttcgctgct agctaaagca aaaacttgtt tcattcttgg tgaatatgga
    15541 agaaagactc ctttagggta agaacggata ccaccataac caatcagatg ttcaatttct
    15601 tcataaattt ccgtttcatt caaatccatt tcacgtaata cttttccagc aattccattt
    15661 tgctcgacca ctaaggctag taaaacatgt tctgaactaa ctgattgatg tttcaaccgt
    15721 tttgcttctt cttgtgcaat ttctaatact gcttgggctc tttcagtaaa aagttcttcc
    15781 atctaatctc acttcccatc ttcataactt aaacgttcca gaaatgctcg gagaatccga
    15841 gcacgtgtat gatcttcata ttcattgaaa ttcaatgtgt tttttgcaat tgcactcaac
    15901 atcaagtttc cttctttttt tgtaattatt tggtcttcat aaagtttttg aataatagca
    15961 aaagcatttt tctcactgat tgtatcatca aataactgat ttatctgctc aagtaattgt
    16021 tttttatctg aaatccttac ttttgcaatt cgtatataac caccaccacc acgtttgctt
    16081 tctactagat aaccacgttg gatggtaaaa cgggtattaa tcacatagtt gatttgtgaa
    16141 ggaacacagt taaagaggtc tgccatttca atccttcgaa tctcgatcat ttcactattt
    16201 tccagaattt tctttatata tgattcaatt aaatccgagg tattctgatg tgccatctta
    16261 ttcattccct ttgactaata ttgaccttaa ttataacgaa tttgataata gaaagaaaga
    16321 agaacgcttt ttgttaaata aatcctttag aatcaacaaa aaaaaatacg gaatatacta
    16381 ttccgtatcg acgcgcccaa gaggagtcga acccctaacc ttttgatccg tagtcaaaca
    16441 ctctatccag ttgagctatg ggcgcatatt caatttaaaa aaattataaa tcatttgaaa
    16501 acaaaaagca agataaaaaa aatcgggaag acaggattcg aacctgcgac cccttggtcc
    16561 caaaccaagt gctctaccaa gctgagctac ttcccgttta aaaaataaaa aagac
//
LOCUS       DAZABD010000056        16583 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000056 DAZABD010000000
VERSION     DAZABD010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16583
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.056"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            185..1183
                     /locus_tag="ACHALG_002044"
     CDS             185..1183
                     /locus_tag="ACHALG_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFN2587417.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            1295..1804
                     /locus_tag="ACHALG_002045"
     CDS             1295..1804
                     /locus_tag="ACHALG_002045"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFN2587418.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(1834..2493)
                     /locus_tag="ACHALG_002046"
     CDS             complement(1834..2493)
                     /locus_tag="ACHALG_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HFN2587419.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(2495..3259)
                     /locus_tag="ACHALG_002047"
     CDS             complement(2495..3259)
                     /locus_tag="ACHALG_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HFN2587420.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(3267..3944)
                     /locus_tag="ACHALG_002048"
     CDS             complement(3267..3944)
                     /locus_tag="ACHALG_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HFN2587421.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(3981..5366)
                     /locus_tag="ACHALG_002049"
     CDS             complement(3981..5366)
                     /locus_tag="ACHALG_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HFN2587422.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(5576..5660)
                     /gene="ffs"
                     /locus_tag="ACHALG_002050"
     ncRNA           complement(5576..5660)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHALG_002050"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(5683..6198)
                     /gene="tadA"
                     /locus_tag="ACHALG_002051"
     CDS             complement(5683..6198)
                     /gene="tadA"
                     /locus_tag="ACHALG_002051"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFN2587423.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            6305..7162
                     /locus_tag="ACHALG_002052"
     CDS             6305..7162
                     /locus_tag="ACHALG_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN2587424.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(7243..7578)
                     /locus_tag="ACHALG_002053"
     CDS             complement(7243..7578)
                     /locus_tag="ACHALG_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587425.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(7645..9015)
                     /locus_tag="ACHALG_002054"
     CDS             complement(7645..9015)
                     /locus_tag="ACHALG_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HFN2587426.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(9093..9629)
                     /locus_tag="ACHALG_002055"
     CDS             complement(9093..9629)
                     /locus_tag="ACHALG_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN2587427.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            9938..11419
                     /locus_tag="ACHALG_002056"
     CDS             9938..11419
                     /locus_tag="ACHALG_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN2587428.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
     gene            complement(11522..12229)
                     /locus_tag="ACHALG_002057"
     CDS             complement(11522..12229)
                     /locus_tag="ACHALG_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HFN2587429.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(12308..12646)
                     /locus_tag="ACHALG_002058"
     CDS             complement(12308..12646)
                     /locus_tag="ACHALG_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFN2587430.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            complement(12757..13719)
                     /locus_tag="ACHALG_002059"
     CDS             complement(12757..13719)
                     /locus_tag="ACHALG_002059"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HFN2587431.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            complement(13734..15065)
                     /locus_tag="ACHALG_002060"
     CDS             complement(13734..15065)
                     /locus_tag="ACHALG_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HFN2587432.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            complement(15073..15753)
                     /locus_tag="ACHALG_002061"
     CDS             complement(15073..15753)
                     /locus_tag="ACHALG_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFN2587433.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            complement(15972..16451)
                     /locus_tag="ACHALG_002062"
     CDS             complement(15972..16451)
                     /locus_tag="ACHALG_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HFN2587434.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
BASE COUNT         4906 a         3452 c         2749 g         5476 t
ORIGIN      
        1 atttgcatac ttcataataa taataacata atacctaacc tctcgtaaaa atactagatt
       61 tgttttcttt tatttttcat cactagatac attctataac ttaccgtttc gatttcgaat
      121 tcaaacattt ttgagaagca ttctcaatta aattctgcta tagtgtgaaa agggaggaat
      181 ttttatggaa atctatgaca ttacaatcat cggtgcaggt cctgtaggga tgtttgccgc
      241 tttttacgca ggaatgcgcc aagctaaaac aaaaatcatt gacagtctac ctcaacttgg
      301 tggtcaatta gcaactttgt atcccgaaaa atatatttac gatattcctg ggtatccagc
      361 aataaaagca agtgaattaa tcgatcaatt agaaaaacaa ttaactactt ttaaccatac
      421 attccattta aaagaagaag ttctctcgct tactcgtgaa gacgaagtta tcgagattac
      481 tacaaataaa ggcatccatt attccaaagc agtcatttta gctttaggaa atggttcatt
      541 ccagccaaga aaattgaatt tagataatgc tgaatcattt gaaaaccacg gattggatta
      601 tttcgtgaat gatttgatga agtatgctgg aaaaaaagtc gctattgctg gaggtggtga
      661 ttctgctatc gattggacgt tgatgttaga gcctattgca agcgaagttt acttgattca
      721 tcgtcgcccg gaatttcgtg cgcacgaaca tagtgtatca cgcttgaaat cttcatctgt
      781 caacttgctt actccctatc tcatcgatgg attatcaggc aataatgggg aactgacaga
      841 tatccggtta aaaaaagtga aaagtgacga gacgattgat ttgatgattg attctctgat
      901 cgtcaattat ggattctctt ctaatttaga acatctttct tcttggggac ttgatagtac
      961 cagaaatgcc attactgtaa aatcagatat gtctacttct attccaggcg tttatgcagc
     1021 tggtgacatc tgtacctatg aaggaaaagt gaaactgatt gctactgggc tcggtgaagc
     1081 acctactgca gtgaacaacg ctttacacta tattaatcca aaggaacgaa ctcagcctgg
     1141 tcacagcaca agcttatacg ataaaaatct tcgtcctagt tgatttttta tatctctttg
     1201 catttcaagt taaacccgaa agcagtttct tgcatttttt tcatgttccg ttaacatgaa
     1261 atagagaaag aaaaaataaa gggggagcaa taatatggtc gtcaaaactg agactttaga
     1321 agaaaaggca agagaactat tgaaggaacg tggcgtaact gtagaagata tcgcacaact
     1381 tgtcatgttt ttacaaaaag actatatttc tgatttgact ttggcggatt gtactgaaag
     1441 tgtcaacagc gtgctaacaa agcgtgaagt acataacgcc attatcactg gaatccaatt
     1501 agatatatta gcagaaaaga aaaaattgat gagtccgttg caacatatta tcgaagatga
     1561 cgaaggcctt tacggtatcg atgagatcct agccttgtca atcgtgaatg tttacggtac
     1621 gatcggcttt acgaattatg gttatatcga caaggtgaaa cctggtattt taaaagaact
     1681 aaacagccac gatggcgttc atgtccatac cttcttagat gatatcgtcg gagcgatcgc
     1741 cgctgctgct gccagtcgat tagcacacaa agaaccagaa aaaagaagtc gccttactca
     1801 ataattcaac aaaaaaagaa agaaaacgtc tatttagacg agttctttct tttttgtttg
     1861 tttcaagcta ttttttccta agaaaagaaa aacagcaaaa aataattcta ataatacaaa
     1921 aaataagata aagctatgca tgaaaaattc ttctggaagt acattgatga ttggatcagt
     1981 aacgggatcg aataaccagt catcgttgtt gaaaaacgtt tcatgaaaaa ggataaaaaa
     2041 gcgatcaaat cctatcatca tgaaaaaacc gaaaacaact ggcagtaaca tgccgatttg
     2101 aaacggacga atcaaccgcc aaagccgatc attcttttgc aaatatttga taaacaaaat
     2161 actcgggatc aaagtgatga aaaacacagc ataatccact aaaaacaaaa ttttcacttc
     2221 ataaaagtga tgggctccgc tagcagaaac tgggaaatct ggcaagctga ggatggtttt
     2281 gaagggatta ttgagataat tcattaggtg atcaaaattt ttcagtaacg tctcttgatc
     2341 taaagaagta taatccaaaa tttgcaaatg tccgatatca aatacgtata gtggtcgaaa
     2401 gttgatcgtc acaccaactg ccagactgat cagtgtcaga aataggctga acaatccagc
     2461 atattccatc cactgccatc tcgaattttt catattagaa gctccattca tccagtgatt
     2521 gtttcacata ggtagctggg ataggcaacc caggtacatc ttcttctttt gtaaaacctg
     2581 ataaaacgag caaagtatct atattattgc gtatcccagc ttggatatct gtttcatagt
     2641 tatctccaac catgattact tcttcttttt gcagccctaa tacttcaacg gctttatcca
     2701 taatgatggc ttctggtttt ccgatgtaga tcggcggcgt ttgggttgct gtctctacaa
     2761 aagagatgac tgaccctgct ccaggaagaa ggccgcgttc agtaggtata ttcttatctg
     2821 ggttcgttcc aatgaaagta gcaccttttt gaatagcaag tgttgcttta actacttttt
     2881 cataggtcag ataattatct aagcctacca ctacatagtc aggagcctct tcttcccatg
     2941 taaatccggc agctaaaatc aagtcaatca atcctgcttc tccgataaca tagacttttt
     3001 tcccttttcc atctgctttc ataaaatcga ttgttgccaa agtagctgtg tagactgtct
     3061 ctggagctac atgaatgtca aactcatctg ctagacgatt agctactgtt tctggtgact
     3121 tagtcgtgtt attggtaaca aacaagaaag gtaattttct tttttgcaat tcttccacaa
     3181 atcgtttacc ggccggtatg ggttgtgtcc cacgataaat cgttccgtct aaatcaatta
     3241 aatatccttt atagtccatt catccactac tcttcccttt ttctgatcgt aaatgctgtt
     3301 tttgtttttt ctacttgttt aggttgttct tgttttttta ctttttcaga ggtttttgct
     3361 gactctgttt ttcgattttt aataaccggt tttttgcgat tagatgcgac tggtcctttt
     3421 ttctcatcga tatacgcacg attatggttt tttttctttt ttcggcgctg agttgtcttt
     3481 tcttttttac cgccgattct ctcgatgaca aaatacgcac aaccaaaatt gcaatattcg
     3541 taaagatagt cttccaagga atcgattcgc tgatctggat gcgcccgtcg attatccgct
     3601 tggaaaaatc cttttaggcg tagttgttca taaccccaat ctcctacgat ataatcatat
     3661 cgggctagta catcactgta acgttctcca agcttttctg cattaaatcc ttcacgataa
     3721 tcggacacaa gccgataatg tctttcacct atcaaaaaat tactgtcatc taacagtgtc
     3781 actagctcgc ctttgacttt ttctgcttta ggctcctcaa cgatttcttc caaagcagct
     3841 gtcagttctt cggtgactgt tgtttcctgt tcagctgatg attttttctt agcaatttct
     3901 gttttatgct caacgttctg ctgttcattt tgttttgcag acatgcgttc accacctatc
     3961 ttttttatta taccttgttt ttatttgatt gggtagtgag ctttcaagta atctcctacc
     4021 acacttcgga taaattcagg gaacaaaaat tcattttttc cagcaatctc aatcgttgga
     4081 aaaaatggaa caaacatcaa atgatctacg gtagtaaaag tataattttt attcagatcg
     4141 attggcttgt tcttccaaaa gacttgatgg ttaatagaat catatgtgat tccattatag
     4201 accatctgcc caaaaatctt accgcgaaat cccattccct tcataggata gttacgtaaa
     4261 aagttacgat ttttttctat ttctaaaact aagcggatca gatcatttcc acccaatgtg
     4321 acatttaaca agtgcatcgg atgcggcaac gcagtgtgaa gctgatcctg attgacccgc
     4381 ccttttggaa tttcagttaa aaacagacca ctgtttaata ttgctgcttc cgtctttcct
     4441 cgttctttca ccgcctcaaa ggctacttgg ataaacgaat gctcattgaa gatatctaga
     4501 ctcagagaaa atggcaaatc tgctacttct ttttctttta ataattcatg cccacgttct
     4561 agataactat ttatttcttg ttcatcttct gtaaaagctg tcattgtttc ggtagggatg
     4621 gctttggctg aatgcttaag gatttttttc tcttcatcga tggttaaatg gacttcaccg
     4681 acatactgcc cgtactttcc agcggctgct atttggacat cattgattac gtgaccttct
     4741 ctgaataaat gatgggtgtg cgatcctaaa ataacatcaa ttgctggtat ttcttttgca
     4801 atttggaaat catcctgaat cccgagatga ctcatcaaaa ctaaaacatc cacttgtgaa
     4861 cgtaaatctt cgactaattc tggcaaaata tcataaggat tccgtatgtc ccagccattc
     4921 ggattataag taagcggaaa tggagcagta aatgcaatta gtccgatttt cgtctggtgt
     4981 ttcgttgtaa tgattttata tggtttcgtc catgccggtg gctggagcgt atttttatcg
     5041 aataagttat ccaggatgat atcaaactca gcatgatcgt ataaatgatt caactcttct
     5101 ttggaatttc ctacgccttc attgtttcct atcgtcgctg catcataacc aacttcattc
     5161 atcagcaaga tattttcctg tccattcgtt gcttctgaca atggatgcca acgatccaca
     5221 aaatcaccta gatctactgc aatcgttgtc gttttttctt tcttttcgtt ttctcgttta
     5281 cgttgatcca aaaaccttct gatccttggc caattttcta aatgagaatg cagatcattc
     5341 gtatgtaaaa tcactatttt ttccatgtac cctctcgcct ctctttatct ttatcatacc
     5401 ataggcagct ttttcttcca ttgaaacaag ctctatttca gagtttttcc tgccttgaag
     5461 caaattgtat aagtacaata ttcgataaat cagtctggct aaaaaatcag gaacattcat
     5521 ttttacagta ctataaaaat aaaaaaaaca gacccgaaag tctgttgtcg tttcattata
     5581 atcagacaca tggcggaacc tgcttagtgc tgcttccttc cagacctgac acgcttcaca
     5641 agcccaccat tgtcctagcc tttcggcaaa gaatagtata ccttattttt cattcgttga
     5701 ccagtctttt tttaatttct tttttcgctg gcggagcttt cggaaaaagt cagtcaaaat
     5761 catcccacat tcgtcttcta aaatgccacc ttctacatat gcttggtggt taaaccgctc
     5821 gtcctccaaa agattcatca aggtaccggc tgttccgcct tttggatcat acgcgccgaa
     5881 ataaacttct gctacacgag acaaaatcat cgcgccgctg cacataggac aaggctctaa
     5941 ggtaacaaaa agtgcagcat cttccaagcg ccagcttccc actttttcac aggcttcctt
     6001 gattgcaagc atttctgcat gtgtagtagc atcttgcgtg gtttctctaa ggttatatcc
     6061 tcttccaatc acttttccat ccaatacaac cactgctcca atcggaactt catgaagcat
     6121 ttctgctttc tttgcttctg ccaatgcaag gcgcatccat ttttcttttt ctactgcatc
     6181 aagatttgta tttaccaatt acccttccct gcctttgttt tttcttgttt tactttagca
     6241 tatgataaaa aggctgtaac taactttttt ttctatggta aaataaatac aagaggtgaa
     6301 caagatgaaa atggataaat tacttgctct ttacccacaa gcacagaaaa aaaactctcc
     6361 agcatcagaa gcggatatcc tttcttttgc aattgatgat gcttttatct ggatcaaaca
     6421 agattcatta tctcaacaag aaaccagcct tttgaaagcg ttattccctg taatcaacga
     6481 tcaaaaaaaa cacccatggt atcactatct ttttaaagat gcttcttgtg ttcaagaaaa
     6541 aagctttcgt ttgatccagc ttcacgtcga atcaaaagga gaattcttaa aaaaagaatg
     6601 gcaagatact attttggaat tatttcgcta ttctgctgat ttcttttttt atacagacac
     6661 agatgcgctc ttagtcgaac aaataaaccg tgattatctt gatgctactg aaatcaacgg
     6721 tatttttcta tctttagatg ctgattttga tacgacaacg tctgtattcg ttggttcttt
     6781 ccattcacct ggcaccgacc tcgtcccttt gtttgctgaa gaaagaaaaa tttttttaac
     6841 agagcaaaac aatctttata ctcacagccg tactttttct atgcaagatg tggctttaca
     6901 ttactataca aaagatactg taaaagatag taagttgatc aaaagttaca tagaactgat
     6961 caaacaaaat gaaatggaag tgatcattca agaattatgg aatgaattag gcaatgtcag
     7021 ctctgctgca aaaacactgt ttcttcatcg taatactcta aaatatcgaa tcgaaaaatt
     7081 tcaagaacag tctggtttca atctaaaaaa agccaacgat ttactgtttt gctatctttt
     7141 attgcttcat gaacataatt aaagatagat taaaggggtt gtgacaataa ttttgtcaca
     7201 accccttatt tcgaatgaat agtgttcaaa agctagtttc tcttacatca ttttttcaac
     7261 aaaatgcatt agccggtctt ttacatcttc ggttgaatct ttgattgaat caccataaac
     7321 agagattttc ttttttcctg atttgatttt tgctaacatt cccatcaaag aatccaagtc
     7381 actatctgaa agaccatcta cgatttctag taatttttca tttccatcaa atttgtcata
     7441 gacgaatttt tttactttcg tccgagtagt catatgatgg atcttttcca ttacttttcc
     7501 cgatacaacg actgctgtcg ctacactggc agcggctgct acgcttaatc cgattgttac
     7561 ttttgttgat gttttcataa gtatcagctc ctttttatct atctccatca tagcatagac
     7621 aaaaaaataa acgaactaat ccactcagtg aaatctcaat cctttaggga aaaagttctt
     7681 tactgttcct tttacttgtt tcccaaatcc gacaggtatc ttatctaata ctagcaaaac
     7741 ccatccttga ttcccatcac gttgaaatgt ttctcctgct gtgtaagatt gccattcctt
     7801 ttgagtgatc ggcaagcaag gtatattctc tatctttttt gtagcaagcg ctaatgcata
     7861 gctaggttca aatcgattct ttttgaaatc tcctaaatgc aaacctgtac gtacgacttt
     7921 taaaccatcg agagaagggg ctaactcagg aacttcccaa agatggtcgt tgaacactaa
     7981 aagtcttcca gtagcttcat aatgaaaatc atttgaaaac tccgtccaga gtttttcctg
     8041 ctcttttgtc atttgcactt tacttttttt tcgtgtcttc ttttctttat gcatctgatt
     8101 ttgtccatga aaagtcagct ttgcaacaaa atgtccttca ccctgatctt tatgaggcca
     8161 tatacgtatt gttttttcca aaccagctac gcttccccat tcagaacgtc ctgaactaac
     8221 ggattgagtc aaaggaatct cctcaatcgt tactggataa ttttctacaa gccagctaat
     8281 gatctcttca ttttcttctg gtgcaaacgt acaagtcgaa taaatcagtt gtcctttatt
     8341 ttttagcatt tttattgctg aagacaaaat ctcttgctga cgtttttgac agtatagcgg
     8401 agattcttct gtccactctt ttattgcatt tggatctttt cggaacattc cttctcctga
     8461 acaaggagca tcaaccacga tccgatcaaa aaagcccgaa aaatgaggga caagttctgc
     8521 gggtgcatga tttgtcacta ttgcgttcga aactccccat cgttctatat tttctgataa
     8581 aatcttcgca cgtttcggaa agatctcatt cgtcactaat aaccctttcc ccttcatttg
     8641 agcagctaat tgggttgatt taccaccggg agccgcacac aaatccaaaa ctttttcacc
     8701 gggttttgct gcagcagctg ttccaacgat cattgcactt ggttcttggc tatattcata
     8761 cccagcttga tggaggaaag actttccatt gacagtgcca agaaatccct cattgctgta
     8821 gggagcgggt tgtaattctt cgctatggta agtttggacc atgtccaatc ctgccggttt
     8881 tagtggattc caccgaaatc cttttttcac gcttccttgt tcaagcgcag aaaaaaaatc
     8941 ggatgcttct tcgcctaaaa gaagacgata ttttttgata aattgctgtg gtaacgttgt
     9001 cgcttcctcc ttcattccaa tgtttcgtta gacattttac aatctttttt acgaaaaata
     9061 aaggtggctg atctactttt tcatgttgct gcctaaggtg actgttcctt taagatattt
     9121 tccaagtatt catccgtcac ctcgttttta tcccaaacag cgttcatcat ttcaagaaaa
     9181 tcttgagaag aatcatctga gcgcaaactt cccaatgcat taagccatac caattcaagc
     9241 gacttttctt ctttgatctt cgtgtgacct ttgacatatg agaaaaatgg ccgcttaaat
     9301 agcgaatcca tgtctaaatc agattttgca atgatttcac gaatactttc atggatagaa
     9361 aaaaaatctt tcaataagta gacaatgctt aaaacatcat cggaactgca ttcagctaag
     9421 tagttttcaa aataaacagt gatttcttct aatatcttct cttttaaccc gctgattgat
     9481 ttgaaatgtg tataaatcgg gtatgtagaa caaccgcaga actctgctaa ctctcttgca
     9541 gtaattgact tcattccata ttcttccacg tataccatac ttttgaacag caaatattct
     9601 ttactataat ttgttactct ccccatcatt cttacctccg tctcttttca tctacccata
     9661 aataaataac ataaataatt ctaaaaatat tattgataat tatcaaaaca tatttagtta
     9721 tatccattaa aaatcaattt ttccgcctta aaaacaagaa tatcttgaca aaatacccta
     9781 ggaaataaac aatagaataa acaaggtaac aaaaaaatta aataacgtta tataaaaaag
     9841 aaggcgacac tctgtgagaa taacacagac tgaaatgact ttccactcac agtttttgtc
     9901 taactatata caaaaacaga ataaaaagga ggtatttatg ctaattgaag caagaaaaaa
     9961 agagttactt gttctacaaa tggtcattca aaaaaataat tgttctttac aagaattatc
    10021 caatgactta catattccaa aacggacaat caaagaactg atccgaaaaa taaatgtatc
    10081 actcaagcaa tttttattga ttgatgaatt tatttattct aatcataaag gagagatcca
    10141 aaccaaagaa accgatcaaa ctaaaaagtt agctgttttt tctaagttaa aattatgcta
    10201 cctaaaagaa tccaatcgtt ttaactattt gatgttacta atcaattatc cacaaactgc
    10261 tgtaccaaaa aaatatttac ttgaacaact ttatatatcg ccctcctatc tcgaaaaact
    10321 gacaaaacag ctcaatcatt ccttaaaaaa attccattta cggatcctat ctgtaaaaaa
    10381 ttgctgtact cttgaaggaa atgagttatc tatccgtctc tatctttact tttttctttc
    10441 agatacattt ggaggtaacg attggccatt ttcctcactt gaattaaatg cactaaaaat
    10501 gaacagtaca gatgagcaaa tagaaaaaat ttcaaaacaa gaaaaaaata tctacttgct
    10561 gttaacttta ttcatattac gaaacaaaca acaggcatat tttccaaaaa cttcttctga
    10621 aatcactgaa ttgatggact tattgcagca tacacatgat tattcagaat actttacttc
    10681 tttttcagtg agtcgccctt gtgaagagac agaaacagag agagcctact ttaattattt
    10741 tttctattgt ttttttccaa atcaaatctc ttttcgacaa aaacaggaga ttggtttttt
    10801 attttccaca agcaaaaacc ttatttgtcg acaagtcggt caattgatga cttacttaac
    10861 tcaacagttt cctctcaacc atttagaaaa taaaatacca atttattttt attttcttgt
    10921 gttggcagtt gtttttttcg aactgttggg tgaatccagc agtagttttt ctacactttg
    10981 ctttccagaa gccacgtcca atcattctag ttcttttata caggaaaaga aattaagaga
    11041 aaaaatcgat acaatttttt caaaaaatgg ggaaagaaac caaatcagtc aataccttag
    11101 tcaattgatc caacacttac tacaaacaga aacgaaagag cagttactca tccatttgca
    11161 gataaccaaa cagtttacag gaaattatca tcttcaaagc catttgcagc agttatttcg
    11221 caaagatgct gttaaaatta ccgcatattc agaacatgct gatttgatca ttacagatca
    11281 gcatgagaaa atttcagatg aaacggagac tttctatctg gatacaacgc acaatacagt
    11341 gttatttggc aagttaataa aagcggttca tgaactgtat atgaaaaaag gttatgagaa
    11401 caaaacaaag aggatgtgac aaaattcttg tcacatcctc ttattccaaa taaatggtat
    11461 tcaatcagct aactctcggt attagctcag atcaaagcgc tgatttcaca acttctttat
    11521 tttaatctct tcggttgccg ccgaacaata agatcaagcg caacagctgc aggaatgtcg
    11581 aaagcgctgc tgcaacataa gtcagtgctg ctgcaaataa tacgtgtctt gccatcggta
    11641 cttcttcttc cgtcagtagt ccgccttcac tcaaaatttg gattgctcgc cgagacgcgt
    11701 taaattcgac tggtaatgtc accaattgga aaagtaatgc taaagaaaaa gcgaaaatcc
    11761 cgatattgat taacgtagag ttccagctaa gcaatacacc taccataatc aaggggaacg
    11821 aaatcatcga accaaagtta gctgctggaa caagtgctgc acgaatcttc aatgggacat
    11881 aacccgaagc atcttgtacc gcatgtccgc attcatgagc tgctacacct atcgcagcaa
    11941 ctgaagtaga ctgtgcagtt gcttgagaca aactcaacat cttattacct gaattgtagt
    12001 tatctgtgag atctccggca atttgttgaa caccgacatt atttatctgt tgactttgta
    12061 atatatattg tgctgcttgt gtaccagtca cattgttttt gcttctcact tgatcgtatt
    12121 ttcggaaagt actattgacg tacgctgacg caattccgga aataactaat ccaagaatga
    12181 ccaaaagaaa tgtcggatct aaaaaaccat acattccata accatacatt ttcgtttcac
    12241 tccttttcgt ttactttatt ttatcattag tagtaggaaa agaatgtgaa aagaccttga
    12301 atattcttta attctttttc acaaattccg atttcagttt catcggtcca aatccttcta
    12361 ccttgcaatc gatattatga tcgccttcta ctaaacggat gttcttgact tttgttcctt
    12421 gtttaatggc acctgacgca ccttttactt tcaagtcttt aatcactgtc acgctatcgc
    12481 cctctaacag acggtttcca tttgcatcta ataccactaa tccgtcttct atgacagttt
    12541 cttgttcttc tttgttccat tcgttcccgc attctgggca aatcaacaag cctcgatctt
    12601 catatgtgta ctctgagcca cattctgggc aatttggtaa tgtcatcttc tttctccttt
    12661 aaaatcttta gtgagcacta ttgtaacgga aaaagaagat cttgaaaaga tacatttgtg
    12721 atttttacta caaaaggcac gttttcgaac gaagtatcat tctgtttttc tgctcttata
    12781 cgattggcat atatcttgga tctctttata gatttcctct acttctgcta aatcttttgc
    12841 attcgcacca cttgctaatg gatggcctcc tccatgatgg cgtttagcca attcattgat
    12901 gatcggcccc ttagaacgta aacgtacgcg gtaataccct gaaggctgct gaacaaaaat
    12961 cccccaagcc agcacttcgt caatcactcc aggaagtgaa acgatcgaag ctgtctctga
    13021 atcatcgatt ccatatttat caagaatttc ttgcggaagg attattcttc ctgcaccatt
    13081 ttcatccacc tggatatttt gataaagata accggaaagt ttagcgactt tcatcggcat
    13141 ttgttcaatc tctctgttca aagcacttgc atcaaatgga taatttctca atatcgaagc
    13201 aatctctaat gtacgggacg tcgtggaggg atacaaaaag cgccctgtat ctccgacgat
    13261 tcctgcataa agtaaccgtg cagccttaac gttcatcttt aaagattcag gaaacatcaa
    13321 agcaaattct gcaatcatct cactacagct acttgctttt gtatttaccc acacaagatc
    13381 tccataaggg tcatcattgg gatgatggtc gattttgatc agtttttctg ccaactgata
    13441 tctttgatca ctgattctgg gtgaattagc tgtatcagtc acgatgacta gcgctccttg
    13501 gtagaagaca tcttcaactt cttgcatttc tgctaaaaag cgaagccctt caatcgtttc
    13561 acctacttga cgtatttctt tttctgggaa actttcccgt aatagttcgg ctaatcctac
    13621 ttgtgatcct aatgcatcag gatcagggcg caaatgcctg tggataatga ttttatcgta
    13681 tttttttatt gtatctacta tttcttgctg tactgtcatt tcactcctgc tttctaagaa
    13741 cgttccatta cttgacaaac aacgattgct ttggaaacga tggtgttttc aataaacact
    13801 tcgatatcca gtttagcgga acgtctgccg atttccaata cacgtggacg aatgtctaat
    13861 tcgctttcca attggatcag ccgcaaataa tgtaggttca cttgttcaat caagatattt
    13921 cgtttttggt tcatcaccat tgttttttgt gcgacatcag aaatgatttc gctaagtacg
    13981 ccaaaagaaa tggttccaac gccattgacc atttgtggtg ctactacgaa tttgaactta
    14041 gggttttcca aacgatttcc ttcacggtca gtatcaatcg tatggatatt tccagatatc
    14101 tgatccgaaa tcgtatctgc aatctgcggt tgtcgctgga caagctgcat cgctttcatt
    14161 acatcttgcc gggtaatcaa gccttccaat gtcaagtcat cggaaacgac cggcatgact
    14221 tctagcccat cccagatcat ttgatggcta acagaggcaa tactcattgt tttctttaca
    14281 ctgattggtt ctttcgtcat cacccgatcg atgatctggt ttggcgattt ccctaaaaca
    14341 tcttttgccg taacaatccc catcaaacga tgatggcggt tgacgactgg aaaacgcgaa
    14401 tgctgcgttt cttctgacaa acgctgataa tcagaaatcg tatctgtctg ctgcaaataa
    14461 tgagtctttt cagctggcat atagatatca ctgacaagca tgatatcttt tttgatcaat
    14521 tggtcgctta acgctcggtt gatcatggtt gcgactgtaa atgtatcata agaggtgcgt
    14581 aagatcggca tttcaagatc atcagctagc tttgcaatct cagcagtcgt attgaatcct
    14641 cctgtgatca atactgcggc gccatcttcc aatgctaatt tttggacttc cgcccggttg
    14701 ccgacgatca ttagtgatcc tggcgtaata tagcgttcca tggcatgttt agtcatcgcg
    14761 ccgatgacga atttattcaa gaccttgtct aatcctgccg ccccgcctaa aacgtcccct
    14821 tcaatgatcc tgacgacctc gccaaatgtc aaacgttcaa tatgtttttt taatttgcgt
    14881 tcgatacgga tggtccctac tcgttgaatt gttgaaacta gtccgacatt ttctgcatct
    14941 ttgattgcac ggtacgctgt tccttcactg acattcaatt cttttgcgat gcttcttaca
    15001 gaaattcgat tgccaacagg taaactttca atatattcta aaatcaaatc atgttttgtc
    15061 gccattttct gcctacactt ccagcgtttc cgagacgctc attatttttc cttcactatc
    15121 ttccagtaac tctacaaaag attcgggatc ttgttcaatg acagggaaag tattgtaatg
    15181 gattggaact actttcttgg cacgaattgt ttgcgcagcc tttgctgcat cttctggccc
    15241 catcgtatag ttatcgccaa tcggcaagaa agcgacatca atatcgaatt cttctgcaag
    15301 caatcctaga tcactgaaat aagcagtatc ccctgcatga taaatcgttt ttccttctgc
    15361 ttgaataata attccgctag gctcgcccat atactgaacg ataccgtcta cttcatagct
    15421 tgaactatgc tgtgcatgta cgaattttac cacgccgaat ggaaacgcat gttttccacc
    15481 gatattcata ccgaatgaat ctactcctct gctgttcgca taatcagcaa tctcaacaat
    15541 actgataatt gtggcttgat tttcttttgc aataggaatc atatctccta tgtggtcact
    15601 atgaccatga gtaatcagca accaatctgt ctttacttct tctgcagtta agtctgtcaa
    15661 tggatttcct gtaataaacg ggtcaatcat cacgcgttgg ccattttcca tttcaattgt
    15721 taaacaggaa tgtccatgat acgtaatttt catattttac acctcgcgtt ttctctgtat
    15781 cttttttatt aaaaacgatt tcatcacttg cacatcaatg aaaaaatata gctgctaaat
    15841 atattttcat tggattgata gtacagtatg tacaaagcaa ttgtaacaga taatacagga
    15901 tgcaccaagt accaagcaga tataatctca agtttgaggt ctctgctctg cttagcttct
    15961 cttctcgtcc cttaactaat taaatcagcg taaataggat ggacgatctt ttctaaatcg
    16021 gctactttta tccgaaggct tctcccgatt tcaccgcttg acacaattac ttcttctttt
    16081 tcttttaaag aactgtctgc taaaaaacaa taatcaggat gtgcgagttt tattccaata
    16141 ggggtgtttg ctccgtgggg atatcctgta tgcattaata caaaatccat tccaggaaaa
    16201 ccaactttac gatctttcgt cacttgtctc gtttttttgt aatctaagtg atcgttcaaa
    16261 ggaatcaatg cgatgatcac cccagttttg tttcctttca ctacgagagt tttacatatc
    16321 aaagctggat caatccctct tttttgtagt tcttctgtga aagattcttc taccacatga
    16381 ctaaaatcaa gtggttgata gctgatcttt tgttcttcta aataacgttc gacttttgtt
    16441 tgactcatca ttgattcact ccctttattc tatcgtttat tataaagcga gggatgctgt
    16501 attcaaaaat tatttgtttt tcgcaaaaaa agagcaagac gagcactcta tagagaggtt
    16561 catcttactc tttttgttta ttt
//
LOCUS       DAZABD010000057        16323 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000057 DAZABD010000000
VERSION     DAZABD010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.057"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            5..2377
                     /locus_tag="ACHALG_002063"
     CDS             5..2377
                     /locus_tag="ACHALG_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFN2587435.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERLENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKDYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQTVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2438..3898
                     /locus_tag="ACHALG_002064"
     CDS             2438..3898
                     /locus_tag="ACHALG_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFN2587436.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVRDDRLDHAVAFLWKDPQTNETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKVI
                     YEEVAKEEKITPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEAINLKD
                     ICREVAKELKACERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIYQQEIRKQKRAMLFQQGRQQERE"
     gene            3909..6113
                     /locus_tag="ACHALG_002065"
     CDS             3909..6113
                     /locus_tag="ACHALG_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HFN2587437.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGTIHQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKTVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGEAVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEATRHLGVCPKCREQEVLLYQNVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNGRTSQSVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNYKKRTRRTTK"
     gene            6129..6278
                     /locus_tag="ACHALG_002066"
     CDS             6129..6278
                     /locus_tag="ACHALG_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587438.1"
                     /translation="MRNTTHKQQEIENQKAKIRLEQEKLRANKRRLQQLKNQQRIQKE
                     IELDR"
     gene            6354..6947
                     /locus_tag="ACHALG_002067"
     CDS             6354..6947
                     /locus_tag="ACHALG_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HFN2587439.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEFEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            6960..7859
                     /locus_tag="ACHALG_002068"
     CDS             6960..7859
                     /locus_tag="ACHALG_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFN2587440.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            7862..8347
                     /gene="ssb"
                     /locus_tag="ACHALG_002069"
     CDS             7862..8347
                     /gene="ssb"
                     /locus_tag="ACHALG_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFN2587441.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSSSPSPTTFEQRDVATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            complement(8488..8592)
                     /locus_tag="ACHALG_002070"
     CDS             complement(8488..8592)
                     /locus_tag="ACHALG_002070"
                     /inference="COORDINATES: protein motif:HMM:NF028245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HFN2587442.1"
                     /translation="MPVETALGLMISFGSLIATIIFGILAVVNNNQKK"
     gene            8766..9026
                     /locus_tag="ACHALG_002071"
     CDS             8766..9026
                     /locus_tag="ACHALG_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587443.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIESMQIRNPDHFTRSMGI"
     gene            9463..9669
                     /locus_tag="ACHALG_002072"
     CDS             9463..9669
                     /locus_tag="ACHALG_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HFN2587444.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYVLIDEKVKKR"
     gene            9669..9920
                     /locus_tag="ACHALG_002073"
     CDS             9669..9920
                     /locus_tag="ACHALG_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587445.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            9935..10372
                     /locus_tag="ACHALG_002074"
     CDS             9935..10372
                     /locus_tag="ACHALG_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587446.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFENIEKNGAFYTIESEEKDNE"
     gene            10365..11072
                     /locus_tag="ACHALG_002075"
     CDS             10365..11072
                     /locus_tag="ACHALG_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587447.1"
                     /translation="MNKVVYQLTTEDVSQFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNRLDVYKRRPDTLKKGAELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELTVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGQ"
     gene            11237..11437
                     /locus_tag="ACHALG_002076"
     CDS             11237..11437
                     /locus_tag="ACHALG_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587448.1"
                     /translation="MNKIIPLLFVLFVGEILMRHGAALHLNFVVFLGILVFCLIFWRF
                     VLTLVSLIFKLIAAIFLIGLFR"
     gene            11453..11632
                     /locus_tag="ACHALG_002077"
     CDS             11453..11632
                     /locus_tag="ACHALG_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587449.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            11763..12032
                     /locus_tag="ACHALG_002078"
     CDS             11763..12032
                     /locus_tag="ACHALG_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFN2587450.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            12025..12282
                     /locus_tag="ACHALG_002079"
     CDS             12025..12282
                     /locus_tag="ACHALG_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFN2587451.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            12741..12896
                     /locus_tag="ACHALG_002080"
     CDS             12741..12896
                     /locus_tag="ACHALG_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587452.1"
                     /translation="MEYKFNLNLKEVLPTQGTININGQDNDDDIVIKKIEFIDSDINV
                     FFILKKN"
     gene            12920..13744
                     /locus_tag="ACHALG_002081"
     CDS             12920..13744
                     /locus_tag="ACHALG_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587453.1"
                     /translation="MIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLELK
                     TLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITACE
                     IGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEKRN
                     KLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSFMYRDFYEESIELTPTEPNKSQ
                     ITLSKELERKVESQELAIGISITNVSTYKELYEAKSQDNKDFVENIDKEPTNTVGD"
     gene            complement(13909..14523)
                     /locus_tag="ACHALG_002082"
     CDS             complement(13909..14523)
                     /locus_tag="ACHALG_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFN2587454.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            14761..15183
                     /locus_tag="ACHALG_002083"
     CDS             14761..15183
                     /locus_tag="ACHALG_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587455.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRDGIAIGNPTNDLTVTENYLIVSPLLALAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            15193..15396
                     /locus_tag="ACHALG_002084"
     CDS             15193..15396
                     /locus_tag="ACHALG_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587456.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            15607..16191
                     /locus_tag="ACHALG_002085"
     CDS             15607..16191
                     /locus_tag="ACHALG_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFN2587457.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT         6058 a         2585 c         3253 g         4427 t
ORIGIN      
        1 ggacatggca gaagaaaaag aaccactaga caatagtcgc ttgggaaaat ccaaaagaaa
       61 attagtacga ttgcaaaatg aattaaacga acaaattgag aagatgtttg aacaccagag
      121 aaagaccaac ggtcagccaa tgaacgataa gagaaatggg cattcttggt ttaggcaaca
      181 agaacgttta gaaaacaaag tgcatagtct gagagaagag atcaagcaac aagaaaaaca
      241 ggtagaaaaa cttgaaagac aagaagagct aaaggaaatg ggctataaca agtacggcgg
      301 cctggatatg acaatcgaaa atattccaag aatcaaagaa gaaatcgaac gctttgaaaa
      361 aggtgaatcg actttttcag ccgctacgat ccgaaaatac caacggaaat tggaaacatt
      421 agaacagctg aaagaaagat cagaaaaagg aaaagaaaat cttcttcctg aagtacaagc
      481 aattattgac agcggacgag tgacgcaatg gaaaaaaaat ccaaccattt attttttgaa
      541 agactatcga aaagttgctt tagaactgaa tgaaaacggg ggatttgagg aatcaaaaaa
      601 ataccaagcc acgtcggaag aggaaaaagc agctgttcgt gaattacttg aagaggctgc
      661 gccaactgat tctttgaagg agaaagaaaa cgatcatttc tatcgagtag aattcaacga
      721 aaatgatcca gaaaaaggat tgaaaagtta tggtggggag atcgtaacat tagaattgat
      781 cgatcaactc aaaacgttgg ataaccaata cgccgcagaa gcaggttatt ataaattcta
      841 ttttgaagaa gtgaagaacg gagaagtagt cgatcagatc cgaatggatc taggggatgg
      901 cgtcaacagt aatcaaccca tctatttaga gttagcaaaa ttgtgcgaac aagtacctga
      961 aaaggaacaa aaacaaacgg tcaaaaatga acctgtttcg aaagaacaaa tgcagtcgaa
     1021 agaaacaaaa acaaaacaaa agggctatca aaaacgaacg gcagaagaga ttaagcaaga
     1081 agtcaaatcc ctttcgcaac aggcactgga tgcggtgaaa aagcataccc attctcctga
     1141 agacgtgaaa gaacttttga attttatgtc tcgttttcca gaacgatcct ttagaaatca
     1201 attattgatt gaggaacaat ttccaggtgc aacggcttgt ttaggtcgtg ctgcgttgaa
     1261 aaaagaagga atctatatca aaaaagggga acaagggaac aagatctttg tcagaaaaac
     1321 ggtcaaagga ttttatgaga aaggacgtgg ctttgttcga gaaacagaag ctacaccaga
     1381 agataaaaaa cgaatcgaaa gtggtcaaat cgaagtgatc aaaaaacctt actacacgat
     1441 tgaaaaagtg tttgatatca cacaaacaca gttaaaacct gaagactatc cgaaaatctt
     1501 tccgaaccgt gtctttgatt ttcaattgga taaaaaagga cagtcagaac ttcaagctgg
     1561 gattcaagcg gtagccaaca atattggaat acaaattcga gatatgacag aaagtgaagt
     1621 ctatcaaaga gaattaggac aagcacgggg ggcgtatgtt cgtaacgaat ttactggcga
     1681 agaagaaatc gtcatgaata ctcgaaatac tcctacgcag catttagcta caagtattca
     1741 tgaattagcc catgcaagaa tgcacaaatt ttcagaatta gataccgcaa ctaaagaatt
     1801 gcaggcagaa atgacttctt atattgtgtg caaacatttt ggaatggaca cttcggagaa
     1861 agctattcct tatattgcag catggacgaa gaatggtcag acgttggatg ataaggaagc
     1921 ggcggaacgt gggaaaatca tgaatgatgt cagccgtgtc gcaaatgaat tcattcaaac
     1981 catttctaat gaaatcaatc aatacagaga aagagaacca gagatccaac cagcagaagt
     2041 gaagcaggag cataaaaata ctgtagaaac ggcagagaat aagaatattg tgcccgttgg
     2101 ttcactttgg atcgacaaga aagacgggaa agtaatggaa gaagatacgg gtcgaacgtt
     2161 acatttgaag gatgcggcgt ttattgaaga tccgcagcat aatcaagtga ttttaaaagg
     2221 aaaaagttat ggaaaagaga ttcaaacggc gttaccttca gaagaatttg cgctaggaaa
     2281 tccattccga ttttcacttg aaacagtgtc agaagtggcc aaacgaaaaa atgaaatcta
     2341 ttatgaacaa caattgtcac aaaaagaacg cgtataaaac aagtaaaaaa gactatctga
     2401 aaaacggata gtcttttttc ttttgggagg tttgaaaatg accgaaaaaa aaatgagtct
     2461 tattgatcga tgtaaacaga tcgatatagt ggattttgct cgaaacaatg gaatggctgt
     2521 agtcaataaa ggacgagact atcgcttaga agaccatgat tcatttgttt ttgatcgaag
     2581 aaaacaacgt ttttattgga acagtcaaaa tattagtggc gacattattg aattagctaa
     2641 actttttttc atagacaaag agatccaaga ctctaagcaa caatttaaag cagccacaga
     2701 ttttattcta aagaatgaag acaaaacaga acgagtagaa aatcttcatt ttgagacaga
     2761 aaagtacaaa gatcaccccg ttgattatca accactcaca gaaaaaggaa ggaattatct
     2821 gaaggaagaa cgaaaactac cagagtggct gattgattat gcagaaaaag aagggttgat
     2881 tgccgaacta aagccaaaac atgaacggca aaatttctta gttcgagacg atcgtttaga
     2941 tcatgcggtc gcctttttat ggaaagatcc acagacaaat gagacagtag gggcgagtta
     3001 tcaaggaacg ttcattgatt atgaacgttt tggtgagaga ggcacatata agcatataga
     3061 taaaaattcg acagcgaatc atggctttaa cctaaagatc ggtgacccta agcagttaaa
     3121 attttttgag agtagtattg atttacttag ctatgctgca ctaaatcgtg atcagttaaa
     3181 cgatacgtgg ttagtttcga tggaagggtt aaaacaccat gtgatcagtc attattttgg
     3241 tgaggcagta tctgaattga gaaagaaaca agcctttcct caatccatcg agatttgtgt
     3301 ggataatgac cgtgcaggac atattttcta cgaaaaagaa caattaatgg gcgcagttga
     3361 tccatttacg aatcagaaag tacgttgtga acgaggaatc gctaatgatt ggcaagtgcc
     3421 aaaagaatac aaagtcattt atgaagaagt cgcaaaagaa gaaaagatca caccagaagc
     3481 tattatggcc atacacaaga cagaaaacaa tctgcaatta acgaaccaat tggtttccgc
     3541 tcacaaagta aatgcgtctt ttggtcagca gctttctgtt aacgattcaa tagaagctat
     3601 taatttgaaa gatatttgta gagaagtcgc aaaagaatta aaagcttgtg aacgcgtgga
     3661 tggtacgtac gattttgatc gtttttatca agaaaaaggg gatattaatg ctcagattct
     3721 tttctcttat aaagcagaac aatactacaa aggctataaa aatcatgaac atgaatttgt
     3781 acctgaagta aaaaaggatt ggaatgatca attgaagcat gaaatctacc aacaagagat
     3841 cagaaagcaa aaacgtgcca tgttgtttca acaaggtaga cagcaagaaa gggagtgaaa
     3901 tcgtcatgat gaaaaccgtt attttagcag aaaaaccgtc acaagcgaag gcttatgccg
     3961 atagcttttc taaagctacc cgaaaagatg ggtattttga gatccaagat cgattgtttt
     4021 caggagaaac tgtgatcacg tatggttttg gtcatttagt cgagttagat tcgcccgata
     4081 tgtacgatga aaattggaaa caatggtcat tagagcattt gcctattttc cctacacatt
     4141 atcactatca tgttcctaaa gataagaaaa agcaattcaa ggtggtgaaa cagcaacttc
     4201 aatcggccga caccattatt attgcgacag atagcgatcg tgaaggggaa ctgatagctt
     4261 ggacgatcat tcaacaagca ggcgccgatc atggaaagat atttaaaaga ctttggatca
     4321 actccttaga aaaagaagcc atttatcagg gattccagca gttacgagat gcagaagaga
     4381 cctacccaaa atttgaagaa gcacaagcac gccagatcgc cgattggttg atcggcatga
     4441 acggtagccc gctgtatagt ttactcttgc agcaaaaagg cataccagga agtttttcct
     4501 taggcagagt ccaaacccca acgctttaca tgatttatca attacaggaa aagattagaa
     4561 acttccaaaa agaaccatat tttgaaggaa aagcccaagt aatcgctcaa aatggcgcat
     4621 ttgatgcgaa gcttgatccg aatgaaaccc aagcgacgca agaagcattt gaagattatt
     4681 taaaagaaaa gggggttcaa ttgggaaaac agccaggtac gatccaccaa gttgaaaccg
     4741 agaaaaaaag tgcggcaagt ccacgtcttt tttctctatc cagccttcaa tctaaaatga
     4801 atcagctcat gaaagctagt gccaaagata ctttggaagc catgcaaggg ttgtatgaag
     4861 ggaaatatct aagctaccca cgaacagata ccccttatat tacagaaggg gagtatgcgt
     4921 atctgttgga tcacttagac gaatacaaac actttttaaa ggccgaagcc attcctactc
     4981 ctattcatac accgaatagt cgatatgtga ataataagaa agtccaggaa cattacgcga
     5041 tcattcctac aaagacggtc atgacggcag cggcttttga acaattaagt ccactgcagc
     5101 aggctattta tgaacaagtg ttaaaaacaa cggtggccat gtttgcggaa aagtatacct
     5161 atgaagaaac aacgatcttg acgcaagttc agcagcttca gctaaagaca gtcggaaaag
     5221 taccagtaga tctaggttgg aaaaagctgt ttggcaaaga gagcgacaga aaagaaaagg
     5281 aagaagaacc gctacttcct aaagtgacaa aaggagaagc ggtgacggta gatctacaag
     5341 tccttgaaaa agaaacgaag ccaccacaac cttataccga aggtacgttg atcacagcga
     5401 tgaaaacagc tggaaaaacc gtggatagcg aagaagcaca atccatattg aaagaagtag
     5461 aaggaatcgg tacagaagca acacgagcaa atattattga aactttgaaa caaaaggaat
     5521 atatcaaggt agaaaaaaat aagcttgtgg tgacaaataa agggatttta ctttgccaag
     5581 ccgtggaaaa agagccgctg ctaacaagtg ccgaaatgac cgccaaatgg gaaagttact
     5641 tattaaagat tggtgagcag aaaggaacac aagctacttt cttagctaat atccaaaaat
     5701 ttgtctctca cttgttggaa gtcgtaccgg gacaaatcca gtcaacagat tttggatcaa
     5761 cgttacaaga ggtgaaagca gccagtgaaa aacaggaagc aacgcgtcat ttaggtgttt
     5821 gtccaaaatg tcgggaacaa gaagtcttgc tttatcaaaa tgtggcggca tgtacgtctg
     5881 aagcctgtga ttttaaatta tggacaacga ttgccaaaaa gaaactcaca gccacacaac
     5941 tgaaagagat cattcaaaac ggccgaacaa gccaatcagt cagaggacta aaaggtcaga
     6001 aaggttcgtt tgaagcaacg atcgttttaa aagaagactt tacaacaggc tttgaatttt
     6061 ctgaaaagaa aaaaacgaac tataagaaaa gaacacgccg cactacaaaa taatgaaggg
     6121 gttatcctat gcgaaataca acacacaaac aacaagaaat cgaaaatcaa aaagcaaaaa
     6181 ttcgtttaga acaagagaaa ttacgtgcaa ataaaagaag actgcaacaa ttaaagaacc
     6241 agcagcgcat tcaaaaagaa atcgaattag atcgatagac gttaatcttg ttacaaataa
     6301 actactatga tatatttgat gtagtgtatt tgtaacgaag ggaggggaag taaatgcaag
     6361 ataaactgaa agaattgttg gctattcgcg atggaaattt atctatgaag gaagccagga
     6421 aatttggaat cgcagcaacc actgtacaaa gattggtgaa tcaaggaaaa ttgatcaaag
     6481 ttgatcgtgg ttattatgta gaggatggtc atggaataga tgatttattt tggcttcagc
     6541 aacgattttc caggggaatc ttctctcatg aaacgacttt agatatttac cagctttcta
     6601 cgaatatgcc gaaattcatt cacttaactt ttccccatgg ctataacgtt gatcgttcaa
     6661 ttacaaaaga gcagcagtta caatttcatt tcgtgaagaa agaagtttat gaactaggaa
     6721 aagtagaggg aacttccttt caaggaaatc ctattactat gtatgataaa gagcgtacac
     6781 tttgtgatat ttggaatccg agatatgaat ttgaatatga aatgaaatta gaagcattaa
     6841 aagagtacat ggaagaccca ttgagagatc caagtaaatt aagagattac atgacaaaac
     6901 tacatgtaga aaaagaaatg aaataccaca tgcaaagctt atattgatgg aggaaaaaga
     6961 tgacaccgac acaatttgca gcaaaaacaa gaaatttagc aaagagtaca ggattaaatg
     7021 cacagttagt gcaacggcat tttatgatgg atcgtctaat tgaacaaatt tctgaatcaa
     7081 agtacaaaaa tgacttcatt ttgaaaggcg gctttttatt aggatcaaaa tatggaatcg
     7141 atagaaggac aacaattgat atcgatacga catttagaaa tagcaaatta actgaagaaa
     7201 aattgaagag catttttaat gatttaacta gaaaacccac aaaagaaggc attgttttct
     7261 ctattcaagg aatgaaggaa acaagagaag ccgattttta tcctggattt caaattaaat
     7321 tggtggctaa tttagagaaa gtacgtgtac cgtttaaaat ggacgtgaca acaggagatt
     7381 ctatttatcc tgaagtagat acacactatc accaattaat gtttgaagat aaaaaagtgg
     7441 agattccagc ttatccaaca gaacaaatta ttgcagaaaa gctaagtgct acgttcagct
     7501 ttggcacaga taattcgaga gcaaaagatt actacgactt atttaccatt ccgaaactag
     7561 aaaatatcga tacgaaagag ttatacaaaa gtgttcgaaa tacatttaca aaacgtgggg
     7621 atacaaaacc gttgtccttt tattatgaaa gagaaatgga taagatcaga ggaaatgact
     7681 atttggaaaa gcagtgggaa aaatatcgct cagttaacac gtttgcgaat tccatttcat
     7741 ttacagatac agtcaacgaa attgatcacc taatgaaaag tgtgatcaaa attgaaaatc
     7801 aagaaagaaa acggcagttt aaaagatcac aacaaatgaa tcaagaaatg gagagataaa
     7861 gatgatcaat aatgtaactt tagtaggacg cttaaccaaa gatccagatt tacgttatac
     7921 cgcgagtggt actgcagtag cgacttttac cctagctgtc aaccgaaatt tcacgaatca
     7981 aaacggcaat cgagaagctg attttattaa ctgtgtgatt tggcgcaaac cagcagaaac
     8041 gatggcgaca ttagctaaaa agggcagttt gatcggcgtt gttggacgga ttcaaacccg
     8101 aacctacgat aaccaacaag gacaacgtgt gtatgtgaca gaagttgtgg ccgataattt
     8161 ccaactatta gaaagtaaag cagcgacaga aagccgtgcg cacgctgatc aatcgtccag
     8221 cagtccaagc cctaccacct ttgaacaacg ggatgtagct acgcctaaca acaatggatt
     8281 gaacgcttca caaaatccat ttggcggaca atcaatcgat attagtgatg atgatttacc
     8341 attctgattg taaaagatca ataaagttgt tataatggat acgaaaagag gagttgcgct
     8401 aacaactccc ctaacagcac cgtttaagac ggtgaccagc ttaatattat ttttgaaaaa
     8461 taatcgtcga ccggctaaag ctgacggtta ttttttttga ttgttgttta caactgctaa
     8521 gattccaaaa atgattgttg cgatcaaaga accgaaacta atcattaatc ctagcgccgt
     8581 ttcaacaggc aaaaggcttc tcctttctgt aagattttgg aagtacgcac atacgcacca
     8641 cctcactttc aatcggatag ccaccgtcac aaactttact gtcttgatca ttatagcata
     8701 tctaaaatcg aataaaaatt ctcaatttta gctgtttttg ctataatttt agcgaggtga
     8761 tcgtgatgaa caaatatgtt ggtttgcttg ataaaatccg agtaataaaa acacaaccat
     8821 tattggttcg ctttacttta caaacaattc atgaatcgat taattgtgta gttgcagata
     8881 ttgaaattat cgataaattg ttaattatgg atgatggaaa atacaatatt gccgtcacag
     8941 gtcatttcaa taaacggaat caattagtca ttgaatcgat gcaaattaga aaccccgatc
     9001 attttacgcg atcgatgggc atataaaaga agagctgcag tcctaaatga ggattgcagc
     9061 tctttttgtt ttacatagaa acaggaaaag acctttttag gaagacacaa gtgccttctc
     9121 aattgaaaaa cctttctctc actccaaccg ccaccgccgt tcgagaagaa aggaagtgaa
     9181 cgttgcacgt tcactaagat atggtgtctt gcaaagcaag acaaaataga aaaaacttcc
     9241 tctcaccaaa aggttcgttc ctgttcgcta tctctttatt gatttgccag accggcaaca
     9301 agttgccttt tatcaccaaa aagcgacaag gacaagggta aatggcacgc tgcgcgccct
     9361 tgtccttgtc gcttttcgtt gattcattct ggccaacaaa tcaataaaga aagaactaac
     9421 gaaaggaaat aaaacctttc ctaagaggag gcagaatcaa tcatgcgaaa gaaatttgat
     9481 tattggggtg ttcccttcag cgaattattc ccaaactatc atgcaccaca tactgtcgaa
     9541 tgcgattgtg gagaaagagc gaaatgtatc aaaagctatc gtttgtatca atgtccaaca
     9601 tgcgggaaaa aatatacctt gtcttatggc gattatgttt tgattgatga gaaagtaaag
     9661 aaacggtgat gaaaacacaa cacaaattat tgactgtaaa agaagtcgtt caaattttgg
     9721 tggacaaaga agtcatttct gaaaaagtag cagaagcttt acttattccg cggtactgga
     9781 cgatagaaac ccctttagta gaggatgcac cgactatgct atttgaagac ctagtgaaga
     9841 ttgcaggata tgaactgcat tatcaagaaa acatggaaac ttatgaaatc gaatattggt
     9901 taaccaaatt agagaactaa aaaggagaaa aaatatggct caaaattact atgacgaatt
     9961 tgtcaaatta cctttggata aaatggcaca aaaaatggag gatatgacgt tcctatacca
    10021 tgaaacacgt gtacccaaaa aacattacaa ggaaaaattg tcagtcgcag tggaagaaat
    10081 gattgaatca ggtgtcgaaa tgaatctcat tgcgacgtac tatcgaacac tggaagaact
    10141 gaaaaaacaa aatggaaaat ggtttttcca agccttacta tgcttggaag taggtgtcaa
    10201 accaagcact atcaaacctt ccgaatatca agccttagaa ctgacttatg caaaattcgc
    10261 acagattaag aaagctaaag caatggctag cgactggcta gatacttttg aaaatatcga
    10321 aaaaaatggt gcattttata cgattgaatc ggaggagaaa gataatgaat aaggtggttt
    10381 atcagctaac aacagaagat gtttcgcaat ttttaacaaa caaagaacac ttgaatgaag
    10441 taatcagacg gattcaaaaa gactttagtt gtatggaaga aatgaagtac tttattcaaa
    10501 gagtggaaga acagttgtat atggaaagtg aaacgagtca aacgaaggtt acagtcagcc
    10561 ataatcgttt agacgtgtat aaacgtcgtc ctgacacatt aaaaaaagga gcggagcttc
    10621 tacgactaga aacagaaaaa tatatcatga atgtttacct agaaaggagt gagctggtcg
    10681 aaaactttgt cagctacacg atttgcagga aacactatta ctcagaatca gaatattatt
    10741 caatttttga gagcatggaa ttaatggaag aagaaatgga agagctaaac agcaaagatg
    10801 cgacggtaga cgattattac cttgtattta ctagattttt gacgtgtggg acggaatcta
    10861 tcgtgttaat gggaatggat tatgaactca ctgtacccga agcatttttg agggaatatg
    10921 tagaagaagt agataaccca ggcgacgact ggtacacgac tttaactgat tttttagaca
    10981 cctacactta cgacgataca gaagaaatcc aaaatgaact tgaccgtaaa cgaatgccat
    11041 atcaacgatt tgaaaggaaa aaaggtcaat aaccatttat accgaaaagt gaggatgtgt
    11101 gaaataagaa caaaaaacct tgaaattaag ggaatggttc ttatttcgca ccaatcgatc
    11161 caaccgcccc cgccgcttag gtggtgcgaa ataagcgggc aaagcccgat aacctaagag
    11221 aaagaaggag atccatatga ataaaatcat tcctctattg ttcgtactgt ttgttggcga
    11281 gatacttatg cgtcatggtg cagctctaca tttaaacttc gtagtattct taggaatact
    11341 ggtattttgt ttgatctttt ggcgctttgt gttaaccctt gtgagtctaa tattcaagtt
    11401 gattgcagcc atatttttaa tagggttatt tcgatgaaag gagagagtac taatgccaat
    11461 tgcaatagga aataaacggt tacccgtgac gttggatgaa aagagacaaa aagaattgca
    11521 gcaactaaag cagaagtacg gcaaaagtga atccaggatt atgtgtattg cgttagattt
    11581 attgattgcc caagaaaaag caggatttga ggtaccagca ctcaaaaagt gacgtcactt
    11641 tttatcctaa aaactaaaag tgatagcact tttaattata agaagttaga atattaatca
    11701 tttgcttaat tgtacaatat aatgtacaat tgttttatag aaataaataa ggggtgaaag
    11761 gaatggaagc agtagcttat tcaaatttcc gccaaaattt acgtagttat atgaaacaag
    11821 ttaatgagga tgctgaaaca cttattgtaa caagtaaaga tgtagaagat acagttgttg
    11881 tattatcaaa aagagattat gattctatgc aagaaacgtt gagaacactt tctaataatt
    11941 acgtcatgga aaaaattcgt cgaggagatg aacaattctc caaaggtgca tttaaaacac
    12001 atgacttaat cgaggttgaa tctgatgatt aaggcttggt ctgatgatgc ttgggatgat
    12061 tatctttatt ggcatgagca aggaaacaaa agcaatataa aaaagattaa caagttaata
    12121 aaagatatcg atcgttcccc ctttgctgga ttaggaaaac ctgagccatt aaagcatgat
    12181 ttatctggaa aatggtccag aagaattaca gatgaacata gactgatata tagagttgaa
    12241 aatgaaacga tatttattta ttctgcaaaa gatcactatt aaccaatcgg aagtaaggaa
    12301 agggtcagaa acttaaaagt ttttgatcct tattttattt accctagtca tttaaaaagc
    12361 taatatagct tagtgttgat tgttattaat gaatgtgttt gttacgcgta ttacggatat
    12421 aaggttagta aaatcatttc taaagttgag gaaaagtaaa tataaatggc ttaaatttca
    12481 acaatttgaa gttgaataga tatgttataa tactattgta gtgtgggatg ttagttacta
    12541 aaggatgacg cttatatata tgactgaata gaataagcaa taggtttaat aatctatttt
    12601 aaattttttg tactagtttt agttaattag caaaaacaac aaaaataaac ttctcataga
    12661 atttagctaa aaattaatga tttatttaca tattaaattt ggatacagtt aagtaatttt
    12721 tatatattgg aggagaagta atggaatata aatttaactt gaatttgaaa gaagtactcc
    12781 ccactcaggg aacaataaac ataaatggtc aagataatga tgacgatata gtaataaaaa
    12841 aaatagaatt tattgattct gatataaatg ttttttttat tctaaagaaa aattagctga
    12901 ttttagcaat attgctgaaa tgattgaacg tttaaaagat tttatgatca gtgtttggga
    12961 tgtagcgatg cttgctgacg aatcaaatta tacttcgagc atatacagat tagaagatag
    13021 taattggata gaaatttcat tagagttaaa gacacttcct aattccgtta actatataat
    13081 tagagatata acttctgaag ctcaacaatt agttaattac atgcatggac acgaaagtat
    13141 ccccttttct cttatactcc ttaaaagaag taaaaatata tcaaatacta gagataggtt
    13201 tatttcgata attacagcct gtgaaatagg ggtaaaggaa ttttatcaac aagagaaacc
    13261 tgatttaact ctaatatttg agaatttaca atctccccct gtagacaaat tattagggag
    13321 catgtttaaa gggtacttta atattgagtt tccgaaagaa ataagaaagg aaattaaaaa
    13381 tttcatagaa aaacgtaata aattggttca ttctcctcaa ggaagtaaac caactataga
    13441 agaatgtttt gaatgttata tggcagtgct aaaaacattt aatttttttt ataaaattaa
    13501 tgatagcttt atgtatagag atttttatga agaatctata gaattgactc caacggaacc
    13561 aaataaatct caaatcactt tgtcaaagga gctagagaga aaagtagagt ctcaagaact
    13621 agctatagga attagtataa ccaatgtgtc gacatacaaa gaattatacg aagctaaatc
    13681 acaagataat aaggattttg tagaaaatat agataaggaa ccaacaaata cagtagggga
    13741 ttaacgtaaa taagtgaaga ttatattaac agactcttta tttagttaag taaaaaatgg
    13801 tttgttggaa ttttatgctt tatctgttct gagtaagact taattttata gatagtaatt
    13861 atatgtagga aattaaagat gagaagataa taaaagcagc atgatcaact atttgtcatt
    13921 gaccatgctg tcttttttta ttctatacac ggtttgtctc gtaacattgt agtctttagc
    13981 aatcttagca atcgcaacac catttttaag atcttcaacg atgcttttgt aaacaaggcg
    14041 acgttgggga tcttttgcgt ctgcactgta taatttgggc cgccctttgt agacgccgtt
    14101 tgcttttgcg atcttaattc cttgtgcctg gcgacgtttc gattcagtcc gttcttgttc
    14161 agcgatcatg gccaaaattt ggatgatcag atccttaatg aatttatcca ggagaggatt
    14221 gccaattgct tcagccatga tcggtaaact agtaatgatc agcttcacat ttttcttctt
    14281 caagtaattg actgaagcga tgatctcgtc ataatttctt ccaagtcgat caatagcttc
    14341 cacaataaaa atatcttgat ctcggacgta atctaatgct tcttggaaaa caggtcgatg
    14401 agtaatcgtt gcgccagatt gtttctcgat gaaaattctc tctgcaccga attttttcat
    14461 ttcttcaatc tggcgttgtt cattttgatc gatcgatgag acacgtatgt atgcaactct
    14521 caaaactttt acctactttc tgcaactatt taaaatacac cctaatcata cacgaggaag
    14581 ccatatgtca cttttctttt taaatggcaa tattttttgt aaataaatgt caatcaagaa
    14641 aatttaaacg attatgacat taaaaatata ttttatgaat tttctagtat ataacttgat
    14701 ttatctgtta agatttatta agaaaacgtt ttcttagttt tgatttcgag gtgatagaca
    14761 atgaaaaata agtttatttt atctttaggt attttaacta ttggaattag tgtaactgga
    14821 actacaattc ctacatttgc tgatagtaca aatgaaccaa ctactgtagt agataataac
    14881 caagaattaa ctcctttaga tagggacgga atagccattg gtaacccaac taacgactta
    14941 actgttactg aaaattattt aatagtttct ccattacttg ctctagcagc ttctaaaacg
    15001 atacgagtga caggaacgat gatttttgat aaagttggtt ggccttctag acctcctcaa
    15061 tcacggaatt ggaaagaaaa aagaaatggt tattggtact atggaaaatt gtatttacaa
    15121 agttataaat cagctggaaa caattggatt gcgacttatt caggaacctt aactagaaag
    15181 tagggatttt ttatgccaga atttgtgaac acaccaaatt tttatcaaga tagcgatatt
    15241 ctaagtaaaa ttaatttacc gaaagaagaa tcagctttac aagttcagcg aactttacag
    15301 gagacacaaa aaattcaaaa atcaatttct aatctagaag attctgaaga aaaaagaaaa
    15361 aaaagagacc aaaaaacata tcaaaaagaa aaataatgat tttaaaaaaa gagtgtctag
    15421 tcgacattct ttttttctaa ttgaaagtgt tcataaaatc acccatttct ggacactttc
    15481 tttttttgct ttatttttca aaaaaacaat tcatttcatg ttcataaacc cataacaata
    15541 tctatgttca gttaaactat atggataaag ttatgttaca ataaaattaa tgaaggagtg
    15601 atttttttga aaataggata cgcccgggta tctactggtt tacaaaattt ggatttacaa
    15661 gaagacagcc taaataaatt tggttgtgaa aaaattttta ctgatcatat gagtggggca
    15721 aaaagtaatc gaccaggatt agaaatggcc attgattttg ttcgctctgg cgatacactt
    15781 gtcgtttggc gcttagatcg tttaggtaga aatatggaag atttgatttc aatcgtcaat
    15841 cgactgaatg agcgtggtgt gagttttcat agtattcaag aaaatattac gatggacaaa
    15901 tcaagttcta caggacagct catgtttcat ttgtttgcgg catttgcaga atttgagcgt
    15961 aatttgatcc tggaacgatc cgcagcagga cgagcagctg ctcgtgcaag aggacggttt
    16021 ggtggtcgtc ctgaaaaatt gtctgaacaa gatttggaat tactgaagac tttagtggat
    16081 agcggtacac ctattaagac tattgcggaa cggtggaacg tctcaagaac caccatttat
    16141 cgttatttgg caaaaatgga ggagaaaaat ggaaagacaa attctcatta gtttttgtca
    16201 tttaatgctg aaaatttttg ccatttatct agaaaagtga caatagtatg agaatttttt
    16261 gtaagtgtct actgataact tattctcaaa tgttgtaagg attttattca tttgttaata
    16321 tta
//
LOCUS       DAZABD010000058        16269 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000058 DAZABD010000000
VERSION     DAZABD010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16269
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.058"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..430
                     /locus_tag="ACHALG_002086"
     CDS             <1..430
                     /locus_tag="ACHALG_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587458.1"
                     /translation="DENGQFFVWMRHEHCWCNPFVLKKENEIRTKIANGEWDYLFRWE
                     DESFIANDMDHAFVSFLDNTWGTWYDCYLEKLGFQNIDIDAIECLKEVDQKYEEEPYY
                     LLQQKLANNSVFQVEIWENEDGTYEGLAQEIPFSDRKSSI"
     gene            605..940
                     /locus_tag="ACHALG_002087"
     CDS             605..940
                     /locus_tag="ACHALG_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587459.1"
                     /translation="MKQYLNEWKAIEGSLVTQRIKELPDCLEKDHLFQIREMLKKEQF
                     DPDQFLVVEYPTIGVYCCNHINDGKYFIIQEYGGQLTPFYTTWEMSEEGINNFPCESI
                     EDSISHTEC"
     gene            complement(1197..2780)
                     /locus_tag="ACHALG_002088"
     CDS             complement(1197..2780)
                     /locus_tag="ACHALG_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587460.1"
                     /translation="MFILDDEIKKVKNPRIIPLLEEVRSSFYQGNYRSAIAVNYSALI
                     LDLIDKLSDLANIYEDNTSKKILEDIARLRKSDPNSPKWEMELLEKAYSSTELIDSYE
                     YEDLKYIKDQRNYSAHPVVTYNGNTWELRDITKETCQDVIRKSYEIIFLKNPILGKKV
                     TIEIIQYASRIHAMSVTPEEFHSALKNSYLNKLSEKAKENLCKTAFKFVFQLSYGNEE
                     ADRNRESTFRLLNALIFDNKEFYLNILKKEIAKYRFTAESREEINKSLGENQQITYTK
                     GFFLLTFIATHPELQHDFSDETIQNLQISINDFKPKQQVLTEIEYYYRLRSLAVLGNN
                     NEELQNHLNSLVDPILQQKMRVSSLPSSTLETIYNQYLYFGEKEMFIDFMIEHLTDAS
                     SFRLADKDMEVLPWIYNKLNQEQFNKLLYNLNGNNQFYKNSNFPPFISNLLDFYNQKF
                     QVNLRKHYSGLLYPNAVTMDQGFQLKDKELKKLYELAESKSDMYLDIIDNIINKSEDY
                     LRKEINSEKELLNYVHKIK"
     gene            2902..3486
                     /locus_tag="ACHALG_002089"
     CDS             2902..3486
                     /locus_tag="ACHALG_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587461.1"
                     /translation="MDLKQSFYIWLYPDNPMSIMADIFVVSILLYLFYLIFFHNLIGY
                     MILDWKGMFDIERKPFANNQTYTVFESGDWEENEVDVIHEIGLALSGIQDADVLVSMT
                     TDTGYNAYEVFYLMYFIWRDGQEIINGRAEKTINSNTEISFCMGEGEMIVYKDGSANI
                     KTAFGDIETSIKELREYVQNPYNFQYLAGRINLV"
     gene            3497..3742
                     /locus_tag="ACHALG_002090"
     CDS             3497..3742
                     /locus_tag="ACHALG_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587462.1"
                     /translation="MKKIINQPTNHLIIEGVIFVTMYFTVGNGISNTIIQKNPQYLLF
                     SLYAWIVQMVWVSILMLLFYWIVRVILLLLNKRKKDR"
     gene            3748..4470
                     /locus_tag="ACHALG_002091"
     CDS             3748..4470
                     /locus_tag="ACHALG_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587463.1"
                     /translation="MKSTKLKVVLLSTAALITLTACGTTNSANNNKDTKSSSTMVTKN
                     STTSASTTMSSMTENNAEKYQKYEDINKGQKPDEVYNNFINTVKNDENYGNDPLQNKN
                     LTIVLYKPDCPDCQKAESKIMQEIKEGFSYAPTLMHDITINKPAYEYYAVAVDITDGV
                     PEWVQNIPGLADENNQYHTPTVAVVTPKKVNGTLYWDVMGMTTNTTDEMIESTTKYYM
                     DLGAYKLYQQSEPVMDDQSKVK"
     gene            4601..5344
                     /locus_tag="ACHALG_002092"
     CDS             4601..5344
                     /locus_tag="ACHALG_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587464.1"
                     /translation="MKEISANEAHIPQKNQVGIYKVLHGEGIYKIQLLTGYNNGANGK
                     WLKATSIRELRSEVEKIRLGLTSEEIAKLKGNNSFVIEKEKEKHLLYRELKIEEYYEI
                     DLPEKLRIIDQRSEEKWKTEIYPSLNDYKTSGKPMYNYTGFLTYTQYKYAFRKLKGIK
                     QAEIALYEMKKVDRLKLIQDLVQNTWNQAIVELEKENGKILTNKYLIVNEIEELIGLF
                     SCEKGEVFLKSSPTYSILDSPYQFIQARR"
     gene            5350..5715
                     /locus_tag="ACHALG_002093"
     CDS             5350..5715
                     /locus_tag="ACHALG_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587465.1"
                     /translation="MSSLKDDYIQAKQEYEKIQMLKSEKVELFLTQKLAEDHIANLDA
                     KTLEEYDRQFNQIHHNIILLVEQAKRRFEKAEYDLLDQGIVCLDKGLRRYYGAELKKQ
                     NVDRNALKHEILELIMQRI"
     gene            5731..6261
                     /locus_tag="ACHALG_002094"
     CDS             5731..6261
                     /locus_tag="ACHALG_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587466.1"
                     /translation="MSQNISAVKQLIEEDLETTVQRLAERIHFANYQTISNWSSRNRN
                     PEEIPMKFFYRIGKEYGVDPIKIFEKTMIYYYHGTPNEVLEHLLEEAKEKSSILELSQ
                     NDKVIQVKCVDPTGKIIVGFDIERKSKIDFITQLKKINNLDILTLRPDHEQLFMSFKK
                     QLKEIQKRYNKVFPNA"
     gene            complement(6857..7336)
                     /locus_tag="ACHALG_002095"
     CDS             complement(6857..7336)
                     /locus_tag="ACHALG_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587467.1"
                     /translation="MTDKTSDLPKLAPKTSRVKSKKDGKTKNAVKKKSVKETSSLLLD
                     IPMSSFDDNTDETSVKIATHVGMNNQNDSFYNLLNSLRELEDKKNQVIASKAPDLTTD
                     LDAEIIQIKQDERSVKSLLKTYFDGFDKELAPYSQAILTKPIQAPHISISLQKKSDD"
     gene            7420..8793
                     /locus_tag="ACHALG_002096"
     CDS             7420..8793
                     /locus_tag="ACHALG_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HFN2587468.1"
                     /translation="MKKSKRKIATLLMLMVQFTGIAMSVITINPQVIHADGIATYDAQ
                     FNCTTEGSGSGTDSDGNDTGSGAVTGDVAKNIEDIYKYMNGEYGLSGASIAGILGNWK
                     QESGIDPRAVEGYYGDGQREVDFIKQFTDGLMGIGYGQWTAGRHTALVDWSKKNNGGS
                     WWTTEAQLDFMFKGDGGFVGILKDYALNASDDVVENAVTFHKNWEISADSESAVIAVR
                     GENAKEIWKYMKSKGMDGAKDESKINKISGGSSTGDGSNNSSSSASGTVEDACATTDD
                     ANSGSGSIGKEGKINGKKGQVIANMTYEEALKKYGKAITLPKFKEPDWSTSPFNGIYQ
                     GQCTELTWAYMSQLYDGNQPTTGNGGYVWESYKSAGAKITNKPIVGYGFSASDGWLWA
                     VGDAGHTGVVIAVFEDGSFLCANFNVPPTQAPTRGVSVTLIDGVDGSDNVHFFSGVGK
                     AKFEIEK"
     gene            8816..9343
                     /locus_tag="ACHALG_002097"
     CDS             8816..9343
                     /locus_tag="ACHALG_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587469.1"
                     /translation="MNSKVKDTIIISLLSGMLLALLVGILFFNKQNQDIEAEKHSVHI
                     EDKLTFFKEVKPNEVDGLRKDLENDIQEMLNGSVQNKFADQSGEEFLRKLFIVTGKPP
                     ITDKSSEKEKIEYYKPFTFKLTNFGIKTSSSGLYEALTDFEVQYNGKKVYDTTLTITL
                     NNDKKVTGGAFYAKS"
     gene            9330..9893
                     /locus_tag="ACHALG_002098"
     CDS             9330..9893
                     /locus_tag="ACHALG_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587470.1"
                     /translation="MQNHEITTAKKAIGILSIIVILCCVVLFQKKNAYDNNVKELEDL
                     KSKQIEVVKNNQKVKAMQEAQYKELGNAEIKEQSRKFFNLFYNWNSWEKYTKNMKEIQ
                     KEFPQIVKSSPVDISGTMVGTGNSPISSYTADYYTTNQKGKMVELITQTRNTPTTATT
                     TIWRGVTSIGENQLFYLEDLDSYVRAE"
     gene            10316..11053
                     /locus_tag="ACHALG_002099"
     CDS             10316..11053
                     /locus_tag="ACHALG_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587471.1"
                     /translation="MADRKLEKLLEETWNPKEFSEFFMENFETDLAVIVKDALREQGY
                     PETANYININFTLYTENKGTWDFWATLANKELSDKSDTGIRNFFESNRDDYMYANHQD
                     KLNFRVEFDETPEEFIERQPPKENVAKVLEDRWNSDEIVSTISELGGQYEPLVEAVRE
                     ELRLNKFPDVQNIDVSQIEINVKITNKLDYGSWADIALEKYIYSTLKEFIENRMDIMY
                     LQHPQYLNFGVEIATPLEEWKMEQGLD"
     gene            complement(11070..11486)
                     /locus_tag="ACHALG_002100"
     CDS             complement(11070..11486)
                     /locus_tag="ACHALG_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587472.1"
                     /translation="MNKKRKLSVLITLVSILTILSGILSLKIFNQINTSSNAKDGIVV
                     ENTSNSKETVTTTKKDKSAEITTKNYLIKTVYFDERKTAILKDEEGSELKLPITVSDK
                     FNGTFSLQTAEGSPDNLKVGQTIILGKNDELYSFVK"
     gene            11878..13125
                     /locus_tag="ACHALG_002101"
     CDS             11878..13125
                     /locus_tag="ACHALG_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFN2587473.1"
                     /translation="MNMKKNSLGMVLFASAVLAGLSVPVTSHADEWTANTPDQIQITT
                     GQSSYAFKKGDTIWSTVEKLKSLGYKTTVAEFAELNNVDLAKGEQRTIPIGRTFNISK
                     DTLTVKEADGEVVSQTVQKDKPDPVENTEQENKKSETAKESEPTANTSDKTETSASSV
                     DGASSSNTNNSTSEKEASQGTSESSTTETDTGTSTNNSQSTSNDANESTSTSSSDQSQ
                     TGSSDETDSSTSPSKPEQPTNVVLGVPFISQGNTMLCEGTSLLEALHYKGVATNQDLM
                     TFVKSMPLSPDNNPNNGFSGEWRHNVDGTYQGMNPAPVVSWGVANGGNVVDISGSSVQ
                     GLKDEIVNGNPVVAWVTYAFEPAQHKSMFWGDAIWNRHVVAVDGFKDGFYHVVDPVFG
                     ASWINAGSFETAYNVSQLAVSVR"
     gene            13395..15785
                     /locus_tag="ACHALG_002102"
     CDS             13395..15785
                     /locus_tag="ACHALG_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFN2587474.1"
                     /translation="MKHEKTIILKSKIIYCFATLVMVLSTLIMPFSAIRAEADVTKPT
                     SAKFTETGVKTDGYIRVNMPNHPNEWMISSQFKDSHGNIGYCMDSELPSPTGSGAGSL
                     KYKGAGSDEFYRMFKGGFPSKTAKELGAGNDTEAWYATQLVSWVLAGNFKVSQIVWSH
                     PNHTAAETARVKKAFEKIYDYAKNGKDTPNTEFSITASKTADEGKYHTFTYKTASNKT
                     GNAKLTFTSAKPAGMKIYDADGKEITNNTVKLNSSFTIKVPVTTPSGTLSFKGTANVS
                     TTNPFTFDGRGVYQDAVVMITTSETKDSKSLSAKWTRAVGQVTVKKVDQDGNALKGAE
                     FTLTDNNNAKSVKTTGDDGLATFDLQQGLTYSLEETKVPEGYQGSFKKDGITVNTDGQ
                     KFSYTATNTIKKGKVKVKKVDQDGKPLANAEFTLTDDSSQTQAEKTDENGLASFDIVA
                     NKTYSLEETGVPTGYTGAFKQEGITVKDDGQTFEYTATNTLKKGVVKVHKVDQDGKPL
                     ANAEFTLTDEKGATQVEKTDENGLASFNIVANKTYSLEETGVPVGYTGAFKQEGISVK
                     DDGQTFEYTATNNLKKGVVKVHKVDQDGKPLADAEFTLTDEKGVKEVKKTDATGLATF
                     NIIANKTYNLEETKVPTGYTGAFKQEGITVKDDGQVFEYTAKNTLKKGVIKVHKVDDQ
                     GKALANAEFTLTDDKGVKVVQKTDRNGLATFNIVANKTYKIEETQNPNGYTGKWSKEG
                     ITVADDGQTFEYTVINHLVPETVRTGGIGTSAFLLVFLIAGLSMVLLYYREQHKAK"
     gene            15834..>16269
                     /locus_tag="ACHALG_002103"
     CDS             15834..>16269
                     /locus_tag="ACHALG_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587475.1"
                     /translation="MKSKKIFSNLMLFGTLLGVGLGASSISSSLGLGGQVAYAQQNYN
                     YQGTGTIHIHKVTNTNGDGNPVSDIDNKGKEIALPNGVKPLEGVEFSYVKLTKDEAKS
                     LGWTDSDTTKGQHIDDAKARLLLNKKYSEVSRMKTDSQGNIDI"
BASE COUNT         5955 a         2273 c         3144 g         4897 t
ORIGIN      
        1 agatgagaat ggacaatttt ttgtttggat gcgacatgag cattgttggt gtaatccttt
       61 tgtgttaaaa aaagaaaatg aaatcagaac taaaattgca aatggtgagt gggattacct
      121 cttcagatgg gaagatgaat cttttatagc aaatgatatg gatcatgcat ttgtctcatt
      181 tctggataat acatggggta cttggtacga ttgttaccta gaaaaattgg gctttcaaaa
      241 tattgatatt gatgctattg aatgtcttaa agaagtcgat caaaagtatg aagaagaacc
      301 ctattatctc ttgcaacaaa aattagccaa taattcagtg tttcaggtag aaatttggga
      361 aaatgaagat ggaacttatg aggggctagc gcaagaaata cccttctcag ataggaagag
      421 ttcgatttga aaagagtata tcagcaattt ttatgaagtg aaagcaagct tacaatatag
      481 taatggaaaa ttgtggttag ctggaaggta tattccagct aaccaaatct tacgttttct
      541 gggagagtgg cgttttaaaa caatttccta ggaaaaatag ttaacataaa aggaatgaat
      601 tgttatgaaa caatatttaa atgaatggaa agcgatagag gggtcattag taacgcaaag
      661 aataaaggaa ttacctgatt gcttagagaa ggatcatttg tttcaaatac gagaaatgtt
      721 gaaaaaagaa cagtttgatc cagaccaatt tttagtagtc gagtatccaa ctattggagt
      781 gtactgttgt aaccatatca atgatggaaa atattttatt attcaggagt atggaggaca
      841 gttaactcct ttctatacta catgggaaat gagtgaagaa ggaatcaata attttccatg
      901 tgaaagcatc gaagattcaa tttcacacac cgaatgttaa ttgattataa ctataaaaaa
      961 ggattgaaca ttatgttcaa tccttttttc aatgaaatgt tttccgccat ttcattgttt
     1021 ggtgcgattt ctattttatt atatcaaaat aaagagagat tctaaagaaa gaagagggaa
     1081 atatataaat caagcgacag tatatttatt attaaatgct attgtgagaa tataactaat
     1141 tagtttaatt tgaaagtaaa tatacgctat aaaagtgcat cttgtgttat aaaaaattat
     1201 tttattttgt gaacataatt taacaattct ttctcggaat tgatttcttt tcttaaatag
     1261 tcttcagact tattaataat attatctatt atgtctagat acatatcact tttcgattca
     1321 gctaattcat acagtttttt taattcttta tcctttaatt gaaaaccctg atccatagtc
     1381 actgcatttg gatatagaag cccactataa tgtttacgta aattcacttg gaatttctga
     1441 ttataaaagt ctaacagatt agaaataaaa ggcggaaaat tactgttttt gtaaaattga
     1501 ttatttccat ttaggttgta tagtaatttg ttaaattgct cctgatttaa tttattgtaa
     1561 atccatggta atacttccat atccttatct gctaacctga atgaactagc atcggtaaga
     1621 tgttcgatca taaaatctat gaacatctct ttttcaccaa aatataaata ttgattatat
     1681 attgtttcta atgttgaaga tggtagcgaa gaaactctca ttttttgctg gagtatagga
     1741 tcaactagac tatttaagtg attttgtaat tcttcattgt tgtttcctag aactgcaagg
     1801 gaacgtaaac ggtagtaata ttctatttct gttagtactt gttgtttcgg tttaaaatca
     1861 tttattgata tttgtaaatt ttgaattgtc tcgtctgaaa aatcatgttg taattctgga
     1921 tgggtagcta taaaagtaag taaaaagaac ccttttgtat acgtgatctg ttgattttca
     1981 ccaagtgatt tattaatttc ctctcttgat tcagctgtga acctatactt tgcaatttct
     2041 ttttttagaa tatttaaata gaattcttta ttatcaaaaa ttagggcatt aagtagtctg
     2101 aaagtacttt ccctatttct atctgcttcc tcatttccat aagataattg aaaaacaaac
     2161 ttaaaggctg tcttacatag gttttctttg gctttttcgc ttagtttatt tagatagcta
     2221 ttttttagtg cagagtgaaa ttcttcaggt gtaacagaca tagcatgtat gcgacttgca
     2281 tattgtataa tttcgatcgt tacttttttt cctaatattg gattttttag gaatataatt
     2341 tcataggact tccgtataac atcctgacaa gtttcttttg ttatatctct taattcccaa
     2401 gtattaccgt tgtaagtgac tacaggatga gctgaataat ttctttgatc cttaatatat
     2461 tttaaatcct catattcata gctatcaatt agctcagttg aactataagc tttttctagt
     2521 agttccattt cccattttgg agagtttgga tcactttttc ttagtctagc tatatcttct
     2581 aaaattttct tagaagtatt atcctcgtaa atatttgcta aatcagataa tttatcaatt
     2641 aaatctaaaa taagcgcact gtaattgaca gctattgcag aacgataatt tccctgataa
     2701 aaactacttc taacttcttc taataatgga ataattctag gatttttaac tttttttatt
     2761 tcatcatcta atataaacat ataaatctcc tttttttatt tgtatgacaa gtttaccata
     2821 aaaaaaaatt aaaaagcgag acatatttat gttgttgtgt tatcataata acataaatat
     2881 gtagagtgag atgatgaaaa aatggatttg aaacaatcgt tttacatctg gttatatcct
     2941 gataatccta tgtcaataat ggctgatatt tttgtagtta gcatcttatt gtatttattt
     3001 tatctaattt tctttcacaa tttaatcggg tacatgattc ttgattggaa aggaatgttc
     3061 gatatagaaa gaaaaccatt tgctaacaat caaacctata ccgtcttcga aagtggagat
     3121 tgggaagaaa atgaagttga tgtgatccat gaaattggat tagctttatc aggaatacaa
     3181 gatgctgatg ttttggtatc aatgacaacg gatactggat acaacgctta tgaggtattt
     3241 tatcttatgt attttatatg gcgtgatggt caagaaatta tcaatggtcg tgctgaaaaa
     3301 acaatcaatt ctaatactga aatttctttt tgtatgggtg aaggggaaat gattgtatat
     3361 aaagatgggt cagcaaacat taaaacagcc tttggtgata ttgaaacaag tattaaggaa
     3421 cttagggaat atgtgcagaa cccatataat tttcaatatt tagcaggaag aataaattta
     3481 gtttaggagt aaaaatatga aaaaaattat aaatcaacca acaaatcact tgataattga
     3541 aggtgttatt ttcgtaacaa tgtattttac tgtaggaaat ggcatctcaa atacaatcat
     3601 tcaaaaaaat ccacaatacc tattatttag tctttatgct tggattgtac aaatggtttg
     3661 ggtttccata ttgatgctgt tgttttattg gatagtacgg gttattcttt tattgttaaa
     3721 caaaagaaaa aaggataggt gaataaaatg aaatcaacta aattgaaggt tgtattgtta
     3781 tcgacagcag cattaataac ccttacagca tgtggtacaa ctaatagtgc taataataac
     3841 aaagacacaa agtctagtag tacgatggtt actaaaaaca gcactacatc ggcatcaaca
     3901 acaatgtctt caatgactga aaataatgct gaaaagtatc aaaaatacga agacattaat
     3961 aaaggtcaaa agccggatga agtttataac aactttatta atacagtaaa aaatgatgaa
     4021 aattatggga atgatccatt gcaaaataaa aatctgacta tcgttcttta caaacctgat
     4081 tgtccagatt gtcaaaaagc agaatcaaaa atcatgcaag aaataaaaga aggatttagt
     4141 tatgctccaa cattaatgca tgatattacc ataaataaac cagcttacga gtattacgca
     4201 gttgctgtag atattactga tggtgtacct gagtgggtac aaaacattcc tggtctggct
     4261 gatgagaaca atcaatatca tacgccaaca gttgcagtag taacaccaaa gaaagttaac
     4321 ggcacactat actgggatgt aatgggaatg acaacaaata caactgatga aatgattgaa
     4381 agtacaacta aatattatat ggatttggga gcatataaat tatatcaaca atcagagcct
     4441 gttatggacg atcaatccaa agttaaatag atctttaggg cgtaagccct atttttttaa
     4501 aattaagtga taacaaataa atgatttctg aaacaaacgt tcttactcaa ctactcgaaa
     4561 cacacaagct aattaactag attataaagg agaatgcgta atgaaagaaa tcagtgcaaa
     4621 tgaagctcat attccgcaaa agaatcaggt tggtatatat aaggtgcttc atggtgaagg
     4681 aatttataaa atacagttac ttaccggtta caataatggt gcaaatggga agtggttaaa
     4741 ggcaacaagt attcgtgaac ttagaagtga agtagaaaaa attaggcttg gtctcacaag
     4801 tgaagaaata gctaaactga agggaaataa ttcttttgta attgaaaaag aaaaggaaaa
     4861 acatctactt tatcgtgagc tgaaaattga agaatactat gaaattgatt tacctgaaaa
     4921 attaagaatt attgatcaac gttcagaaga aaaatggaaa actgaaatat atccaagctt
     4981 aaatgactac aagacatcag gaaagcccat gtataactac acaggctttt taacctacac
     5041 acaatacaaa tatgcctttc gcaaattaaa aggcattaaa caagctgaaa ttgctctgta
     5101 tgagatgaaa aaagtggata gattgaaatt gattcaagat ttagtgcaaa atacatggaa
     5161 tcaagcgatt gtcgagctag aaaaagaaaa tgggaaaatt ttaactaata aatatctgat
     5221 cgtaaatgaa attgaagaat taattggttt attttcatgt gaaaaaggtg aagtcttctt
     5281 gaagtcatca ccaacctatt caattttgga cagtccgtat cagtttatac aggcaaggag
     5341 atgagttaga tgtctagctt gaaagatgac tatatccaag caaaacaaga gtatgaaaaa
     5401 atacagatgt tgaaatctga gaaagtcgaa ttatttttaa cacaaaaatt agcagaggat
     5461 catattgcta acttagatgc taaaacttta gaggaatatg atcggcagtt taatcagata
     5521 catcacaata tcattttgct tgttgagcag gcaaagcgaa ggtttgaaaa agcggaatat
     5581 gatctattag atcaaggaat tgtatgcttg gataagggat tgcgaagata ttatggtgca
     5641 gagttaaaaa agcaaaatgt agatagaaat gcactaaaac atgaaatctt agaattgata
     5701 atgcaacgaa tttgaaggga tagaaagcaa atgtcacaaa acattagtgc tgtaaaacaa
     5761 ttaatagaag aagatttaga gaccacagtt cagagattag cggaacgaat ccattttgca
     5821 aattatcaaa ccatctctaa ctggtcaagc aggaacagaa atccggaaga aattccaatg
     5881 aaattttttt atcgaatcgg aaaagaatat ggtgttgatc cgataaaaat tttcgagaag
     5941 accatgattt attactatca tggaacacct aacgaagtgt tagaacatct tttggaagag
     6001 gcaaaagaaa aatcatcaat attagagcta tcgcaaaatg acaaagttat tcaagtgaaa
     6061 tgcgttgatc caacaggaaa gattattgtc ggttttgata ttgaaagaaa atccaaaata
     6121 gattttatta cacaattaaa aaagataaat aatttagata ttctaacgct tagaccagat
     6181 catgaacagt tgtttatgag ttttaaaaaa caattgaaag aaatacaaaa aagatataac
     6241 aaggtgtttc ctaatgcgtg aaaagctcaa agaaatcaga gttttttgga taattaacaa
     6301 agaatgtatc atcttatata ctcttggatt tcttagcatg atcttgtttt ggtggttata
     6361 tttaaaacag taattggaga gggtcaagag gatttttatc aagataagga tgtggaaact
     6421 gcatgtggaa taatagatta aaaacaggat tgttattaat agttatttcc tgcgctatga
     6481 tgattggtat gaggatacaa agggaacaat cctattttga agtttctgct aacaacgtaa
     6541 tagaaaaatg ctattacggt caacattatt ggagtgagga agtcagagaa aatatagatc
     6601 gtgaatatgt tcaaagaatt gttagattac cagtaagtga actaaaaaaa tgatgagatt
     6661 atcagcatat cttttaatgt ttttgaatgt ggtcacgtta atctttgtag taatcggtgt
     6721 gactttcgat ttaatggaat agtgggaaat ctataatgga aaaaagagac acgaatgaac
     6781 atattttttg aatcaatatt gttgatggtt tttgcgatca gcgcttacca gaaatctaaa
     6841 aaagagtaat gattgtttaa tcatcactct ttttttgtag ggaaattgaa atatgtggag
     6901 cttgtattgg tttagttagg attgcttggc tataaggagc tagttcttta tcaaaaccat
     6961 caaagtaggt tttcagtagc gatttaactg atcgctcatc ttgtttgatt tggataattt
     7021 cagcatccaa atcggttgtg agatcaggag cttttgatgc gattacctga ttttttttat
     7081 cttccaactc tcttaggctg tttaacaagt tgtaaaaaga atcattttga ttgttcattc
     7141 caacgtgagt tgcaatttta acagaagttt cgtccgtatt gtcatcaaaa gaactcattg
     7201 gaatatcaag tagtaacgag gatgtttctt ttactgactt tttctttaca gcattttttg
     7261 tttttccatc ttttttgctc tttactcgag atgtttttgg agctagttta ggtaaatcac
     7321 ttgttttatc agtcatttat tagcttttcc tttctattaa tttatgttat tatattatca
     7381 taataacata aaaaagtaca ctttttgggg gcgtagttat tgaaaaaatc taagagaaag
     7441 attgccacat tgcttatgtt aatggtgcaa tttacaggga ttgcaatgag tgtaatcaca
     7501 ataaatccgc aagtgataca tgctgatggg atagctactt atgatgcaca atttaactgt
     7561 actactgaag gtagtgggtc aggaacagat agcgatggaa acgatactgg aagtggcgct
     7621 gtaacaggtg atgtagccaa aaatattgaa gatatttaca aatacatgaa tggagaatat
     7681 ggcttatcag gtgctagtat tgcaggtatt ttaggtaact ggaaacaaga atcaggtatt
     7741 gatcctcgag cagtagaagg gtattacgga gatggacaga gagaagttga cttcatcaaa
     7801 caatttactg atggtctgat gggtattggc tatggtcaat ggacagctgg gcgacataca
     7861 gcattagttg attggtctaa gaaaaataat ggcggttctt ggtggactac agaagcccaa
     7921 ttggacttta tgttcaaagg tgatggggga ttcgtaggaa tattaaaaga ttatgcttta
     7981 aatgcttctg atgatgtcgt agaaaatgca gtaacttttc ataaaaactg ggaaatttca
     8041 gctgattccg aatcagctgt aatagctgta cgtggtgaaa atgcaaaaga aatctggaag
     8101 tacatgaaaa gcaagggtat ggacggtgca aaagatgaaa gtaagatcaa taaaatatcc
     8161 ggaggttcaa gtaccggaga tggatcgaat aattcatcaa gttcagcatc aggaacagta
     8221 gaagatgcct gtgcaactac ggatgacgct aattctggtt ctggatcaat tgggaaagaa
     8281 ggaaaaatta acggtaaaaa aggtcaagta atagcgaata tgacttatga agaagctttg
     8341 aaaaaatatg ggaaagcgat cacgctacca aaattcaaag aaccagattg gtcaacttca
     8401 ccttttaacg gaatatatca aggtcagtgt acagagttga cgtgggctta tatgagccaa
     8461 ttatacgatg ggaatcaacc gactactgga aatggtggtt atgtatggga aagctacaaa
     8521 tcggcaggag caaaaataac gaacaaacca atcgtaggtt atgggtttag tgcttcagat
     8581 ggatggttat gggctgttgg cgatgcaggg catactggtg tagttatagc ggtgtttgaa
     8641 gatgggtcat ttctatgtgc taactttaat gttccaccaa cccaagctcc aacaagggga
     8701 gtttcggtaa ctttgattga tggagtggac ggatcagata atgtgcattt ctttagcggt
     8761 gtaggaaaag ctaaatttga aatcgaaaaa taaattggaa tgaaggacga tatagatgaa
     8821 tagtaaagtg aaagatacaa ttatcatttc tttgctttct ggtatgttat tagcactgct
     8881 tgttggaatc ctattcttca ataagcagaa tcaggacata gaagctgaaa aacacagtgt
     8941 gcatattgaa gataagctga cattctttaa agaggttaaa ccaaatgagg tggatggatt
     9001 acggaaagat ttagaaaatg atattcaaga aatgttgaat ggatcagttc aaaataagtt
     9061 tgcagatcaa tcaggtgaag aatttttgag aaaactattt attgtaacag gtaaaccacc
     9121 aattactgat aagagtagtg agaaagaaaa aatcgagtac tataagcctt ttacttttaa
     9181 gctaacaaac tttggaatta agacatctag tagtgggttg tatgaagcat tgaccgattt
     9241 tgaagttcaa tacaatggaa aaaaagtgta tgataccaca ttaaccatca cactaaataa
     9301 tgataagaaa gtgactgggg gtgcttttta tgcaaaatca tgaaatcact actgctaaaa
     9361 aggcgatcgg aatactttcc ataattgtga tcctttgttg tgtggtacta tttcaaaaga
     9421 aaaatgctta tgataataat gtcaaagaat tggaagacct taaaagtaaa caaatcgaag
     9481 tagtcaagaa taaccagaaa gtgaaagcta tgcaggaagc tcaatacaaa gaattgggga
     9541 atgctgaaat caaggaacaa agtaggaaat tctttaattt attttacaac tggaattcat
     9601 gggaaaaata tacgaagaat atgaaggaaa tacaaaaaga atttcctcag atagtaaagt
     9661 ctagtcctgt tgatatttct ggcacgatgg taggcactgg taatagtcca atatctagct
     9721 atactgctga ttactataca acaaatcaaa aaggtaaaat ggttgaattg attacacaga
     9781 caagaaatac tccaacaaca gcaacaacaa caatttggcg tggagtaaca agcataggag
     9841 aaaatcaatt gttttacttg gaggatttag atagctatgt aagagcggag taaaataaaa
     9901 aaagagctaa cggtaccaac gttagctctt tgtgctgacg atgataacaa aacctaccga
     9961 aaaaggattg tttcatcaca aacacaaaca gaaaagaatt gataaaaaac ccgaacctca
    10021 gaatttttat taatcaattc atttagatta taataaattg agctgaacat tacaatacgt
    10081 atatgttgta aaacgtaaaa aaagtcaaaa gtaaatactt tcttaaaaag tcaaataaaa
    10141 ctattaaaga caatatatat ggtttatttg acataagaaa atggtctaga tgataattgt
    10201 aatatagaat tgtaggctta aataaagcat aaaatacctt ttatgataag gtatagctct
    10261 taattttaag cagtttttag atataagagt gaaagtaaag tgggggagaa taataatggc
    10321 agatcgtaaa ttagaaaagt tattagaaga aacgtggaat ccaaaagaat ttagtgaatt
    10381 ttttatggag aattttgaaa ctgacttagc agtgattgtt aaagatgctc ttagagagca
    10441 agggtatcct gaaactgcaa actatataaa cattaatttt actttgtata ctgaaaataa
    10501 aggaacttgg gatttttggg ctactttagc aaataaggag ttaagtgata aatcagacac
    10561 aggaattcgt aacttttttg aatcaaacag agatgattat atgtatgcta accatcaaga
    10621 taaattgaac tttagagtag agtttgatga aacacctgaa gaatttatag aaagacaacc
    10681 gcctaaagaa aatgtagcaa aggtattgga agatcgttgg aattcagatg aaatagtttc
    10741 aacaattagc gaactaggcg gacaatatga accgctagta gaagcggtta gagaagagct
    10801 aagactgaat aagtttcctg atgttcaaaa tattgatgtt tctcaaattg aaataaacgt
    10861 caagatcacg aataaacttg attatggtag ttgggcagat atagcattag aaaaatacat
    10921 ctactcaacg ttaaaagagt ttatcgaaaa tagaatggat atcatgtatc tacagcatcc
    10981 acagtattta aactttggtg ttgaaatagc tacacctctt gaagaatgga aaatggagca
    11041 agggcttgac tagcctttgc tccatttttt tattttacaa atgaatacag ttcgtcgttt
    11101 ttaccaagaa tgattgtttg cccaactttg agattgtcag gtgatccttc agcggtttgt
    11161 aaggagaacg ttccgttgaa cttatcggat accgttatag gtagctttaa ttctgatcct
    11221 tcctcatctt tgagaattgc tgtttttcgt tcatcaaaat aaacagtctt tattaaataa
    11281 tttttcgtag tgatctcagc agatttgtct ttttttgtag tagtaacagt ttctttagaa
    11341 ttagaggtat tttctacgac tattccatct ttggcatttg agcttgtgtt tatttgattg
    11401 aatattttca gcgacaagat accgctaaga atagtcaata tagaaacaag tgtaattaat
    11461 actgataatt tccttttttt gttcattttt tcttaacttc ctttttgttt tgattagtta
    11521 gatattcctt atctaacaag cctttttagg ctattaaatg aagataaacc ccaaaaaaat
    11581 attgcaatta tgacagtgca atggtactat tattatacaa taaattttgc gaaaggggta
    11641 ggtctaaatc gcaaacaaaa agaaattgaa cctagacaaa tacaacttgt acaccgcaag
    11701 ggaagcaagt ttgattttgg gtaaaggtag aaattatgtt acaaacataa tcaactatta
    11761 tccaaatcgt ttgcctaaag gttcgttcaa gaaagtgggt gacaaaacct atattataac
    11821 ggaagaagga atagaagcat tgcgatcaca aacagaaaat aaaaacaaaa agaaggaatg
    11881 aatatgaaga aaaattcgct tggtatggtt ttatttgcat cagctgtact tgcggggtta
    11941 tcagtaccag taacctcaca tgctgatgaa tggactgcaa acacaccgga tcaaatccaa
    12001 attacaacag gacaatcaag ttatgcgttc aaaaaaggag atactatttg gtcaactgta
    12061 gagaagctta aaagcttagg ttacaagact actgttgctg agtttgccga attgaacaat
    12121 gttgatttgg caaaaggaga acaacggact atcccaattg gacgaacttt caacatcagt
    12181 aaagatacat taacagttaa ggaagcagat ggcgaagtag ttagtcaaac tgttcaaaaa
    12241 gataaacctg atcctgttga aaatacggag caagagaaca aaaaatccga gactgcaaaa
    12301 gaatcggaac caacagcaaa cacttctgat aagacagaaa caagtgcttc aagtgttgat
    12361 ggagcaagtt catctaatac aaataattct acaagtgaga aagaagcatc acagggaaca
    12421 agtgaaagta gcactacgga aactgatact ggaacatcaa caaataactc acaatcaaca
    12481 tcaaatgatg caaatgaatc aaccagtaca tcttctagtg atcaatcaca aacaggttcg
    12541 agtgatgaaa ctgacagttc tacttcacca agtaaacccg aacaaccaac taatgttgtg
    12601 ctaggcgttc catttattag tcaaggtaac acaatgcttt gcgaaggaac atctttgcta
    12661 gaagcgttac attataaagg tgttgcaaca aatcaagatt taatgacttt tgttaaatca
    12721 atgccactat ctcctgataa taatccaaat aatggttttt ctggtgaatg gagacacaat
    12781 gtggatggaa cttatcaagg tatgaatccg gctcctgtag tttcatgggg tgttgctaat
    12841 ggcggaaatg ttgtagatat ttctggttca tcggtacaag gattaaaaga tgagattgta
    12901 aatggaaatc ctgtagtagc gtgggtaact tatgcctttg aacctgctca acataaatca
    12961 atgttttggg gcgatgctat ttggaatcga catgtagtag ccgttgatgg atttaaagac
    13021 ggtttttacc atgttgtcga tcctgtgttt ggggcaagtt ggattaatgc aggatcattt
    13081 gaaacagcat ataacgtatc tcaattagcg gtttcggtac gttaataaaa gagattgttt
    13141 atatactaag tagttaagta tagtctttat ttgtccttgc ttaactactt agaaaacttg
    13201 ttgctcatgg tgggtagaac taaaaggggt gatgctatga atactagagc gttgaaacag
    13261 cttgctttca acgaaaaaag tgattaatag gtgtggtgat tttaacttag gaatgggcgc
    13321 gaaggtcatt accacaccga catatcgtgg ctcattttct tcgcctaact ggtataaaga
    13381 aggagtaacg agagatgaag catgaaaaaa cgataatttt aaaatcaaaa ataatttatt
    13441 gttttgcgac tttagtaatg gtgttatcta cgcttataat gcctttttca gcaataagag
    13501 ctgaagcaga tgtcacaaaa ccgacaagtg caaaatttac agaaactggc gtaaaaacag
    13561 acggttatat tagggttaat atgccgaatc atccaaatga atggatgatt tcaagtcaat
    13621 ttaaagattc tcatggaaat attggatatt gtatggattc agaattgcca tccccaacag
    13681 gaagtggagc cggaagcttg aagtacaagg gagctggcag tgatgagttc tatcgtatgt
    13741 ttaaaggtgg ttttccatca aaaacagcaa aggaactggg tgcaggaaat gatacagaag
    13801 catggtatgc aacacaatta gtttcatggg ttttagccgg aaactttaaa gtttcacaga
    13861 ttgtatggtc acatccaaac cataccgccg cagaaacagc tcgtgtaaaa aaagcatttg
    13921 aaaaaattta tgactatgct aagaatggaa aagatacgcc caacactgag ttttctatca
    13981 cagcttctaa gacagctgac gaaggaaaat atcacacttt cacatataaa acagcatcaa
    14041 ataagactgg taatgcgaag ctcactttta catcagcaaa accagctggt atgaagattt
    14101 atgatgcaga tggtaaagaa attactaaca acactgtgaa attaaattca agttttacta
    14161 ttaaagtacc tgtgactaca ccaagtggta ctttgtcatt taaaggtact gctaatgtta
    14221 gcacaaccaa tccttttact tttgatggac gaggcgtgta tcaagatgcc gtagttatga
    14281 tcactacttc agaaacaaag gactctaaat ctttgagtgc taaatggaca agagctgttg
    14341 gtcaggtaac agttaagaaa gttgatcaag atggaaatgc tttaaaagga gcggagttca
    14401 ctctaactga caataacaat gctaaatctg ttaagacaac cggagatgat gggttagcta
    14461 cgtttgattt gcaacaaggc ttaacataca gcttagaaga aacaaaagtt cctgaaggtt
    14521 atcaaggttc atttaaaaag gatggtatta ctgttaatac cgatggacaa aaatttagtt
    14581 acactgctac gaacacaatt aaaaaaggta aagtaaaagt taaaaaggtt gaccaagatg
    14641 gaaaaccatt agctaatgct gaatttacct tgacggatga ttcatcccaa acacaagcag
    14701 aaaaaacaga tgaaaacgga cttgcaagct tcgatattgt agcaaacaag acctacagcc
    14761 ttgaagagac cggagtacca actggttata caggcgcatt caagcaagaa ggaattaccg
    14821 ttaaagatga tggacaaact tttgaatata cagctacaaa cactttgaaa aaaggtgtag
    14881 taaaagttca taaagtcgat caagatggaa aacctttggc aaatgcagag ttcactttaa
    14941 ccgatgaaaa aggtgcaaca caagtagaaa aaacagatga aaatggactt gcaagcttca
    15001 atattgtagc aaacaagacg tatagtctag aagaaaccgg agtaccagta ggttacacag
    15061 gtgcatttaa gcaagagggt atcagtgtta aagacgatgg gcaaactttt gaatatacag
    15121 ctacaaataa tttgaaaaaa ggtgtagtaa aagttcataa agtcgatcaa gatggaaaac
    15181 ctttagcaga tgcagaattc accttaacgg atgaaaaagg tgtaaaagaa gttaaaaaga
    15241 cggatgcaac tggtctggct acgttcaaca ttattgcgaa caaaacttac aacttagaag
    15301 aaacaaaagt accaacaggt tacacaggcg cattcaaaca agaaggaatt accgtcaaag
    15361 atgatggaca agtctttgaa tatacagcta aaaacacttt gaaaaaaggt gtgatcaaag
    15421 ttcataaagt cgatgatcaa gggaaagcat tagcaaacgc agaattcact ttgacggacg
    15481 acaaaggtgt aaaagttgta caaaaaacgg acagaaatgg cttggcaaca tttaatattg
    15541 ttgccaacaa aacgtacaaa atcgaagaaa cacaaaaccc taatggttat acagggaaat
    15601 ggtctaaaga aggcatcacc gttgctgacg atggacaaac ctttgaatat acagtaatta
    15661 atcaccttgt acctgaaaca gtaagaacag gcggaatcgg tacatcagcc ttccttcttg
    15721 tatttctaat tgcaggattg agtatggtgc tactgtatta tcgagaacag cacaaagcaa
    15781 aataaaaaag tgttaatgga aaagaagtaa atatgaaaaa agaaggctga cgtatgaaat
    15841 caaaaaaaat tttttcaaat ttaatgctat ttggtacttt gctaggagtg ggattgggcg
    15901 caagctcaat ctcttctagc cttggactag gggggcaagt agcatatgca caacaaaact
    15961 ataattatca aggaactgga acgattcata tccataaagt aacaaatact aatggggatg
    16021 ggaaccctgt ctcagatatt gataacaaag ggaaagaaat agctttacca aatggtgtaa
    16081 aacctcttga aggcgttgag ttctcttatg tgaaacttac taaagatgaa gctaaatcct
    16141 tgggatggac ggatagtgat actacaaaag gtcaacatat tgatgatgct aaagcacgat
    16201 tattactaaa caaaaaatac agtgaagtaa gtagaatgaa gactgattca caagggaata
    16261 tagacatac
//
LOCUS       DAZABD010000059        16099 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000059 DAZABD010000000
VERSION     DAZABD010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16099
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.059"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(229..1956)
                     /locus_tag="ACHALG_002104"
     CDS             complement(229..1956)
                     /locus_tag="ACHALG_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HFN2587476.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(1949..3142)
                     /locus_tag="ACHALG_002105"
     CDS             complement(1949..3142)
                     /locus_tag="ACHALG_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HFN2587477.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3329..3973)
                     /locus_tag="ACHALG_002106"
     CDS             complement(3329..3973)
                     /locus_tag="ACHALG_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFN2587478.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4067..4201)
                     /locus_tag="ACHALG_002107"
     CDS             complement(4067..4201)
                     /locus_tag="ACHALG_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HFN2587479.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4203..6334)
                     /gene="feoB"
                     /locus_tag="ACHALG_002108"
                     /pseudo
     CDS             complement(4203..6334)
                     /gene="feoB"
                     /locus_tag="ACHALG_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
     gene            complement(6331..6804)
                     /locus_tag="ACHALG_002109"
     CDS             complement(6331..6804)
                     /locus_tag="ACHALG_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFN2587480.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7205..7429
                     /gene="nrdH"
                     /locus_tag="ACHALG_002110"
     CDS             7205..7429
                     /gene="nrdH"
                     /locus_tag="ACHALG_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFN2587481.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            7588..9747
                     /gene="nrdE"
                     /locus_tag="ACHALG_002111"
     CDS             7588..9747
                     /gene="nrdE"
                     /locus_tag="ACHALG_002111"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFN2587482.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            9797..10762
                     /gene="nrdF"
                     /locus_tag="ACHALG_002112"
     CDS             9797..10762
                     /gene="nrdF"
                     /locus_tag="ACHALG_002112"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFN2587483.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(10851..11990)
                     /locus_tag="ACHALG_002113"
     CDS             complement(10851..11990)
                     /locus_tag="ACHALG_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFN2587484.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12299..13012)
                     /locus_tag="ACHALG_002114"
     CDS             complement(12299..13012)
                     /locus_tag="ACHALG_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFN2587485.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13266..13967)
                     /gene="nagB"
                     /locus_tag="ACHALG_002115"
     CDS             complement(13266..13967)
                     /gene="nagB"
                     /locus_tag="ACHALG_002115"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFN2587486.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14201..15733
                     /locus_tag="ACHALG_002116"
     CDS             14201..15733
                     /locus_tag="ACHALG_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFN2587487.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(15924..>16099)
                     /locus_tag="ACHALG_002117"
     CDS             complement(15924..>16099)
                     /locus_tag="ACHALG_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFN2587488.1"
                     /translation="FMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQT
                     VVFKGKRNLENKK"
BASE COUNT         4982 a         3334 c         2822 g         4961 t
ORIGIN      
        1 acttcgtaca taataaactt taatcacatt agtctttcat acctacgatc gatctctttg
       61 actcgcaccc ataaattcat aatagtttgt cggcgtcttt gaacgtttcg aagatgagct
      121 acgcattctc tttttttata gacaaaaaag atagagacga acatggagct tccaaatttg
      181 gatatttgat ccaattttgg aagctccatt tatttcgtgt ctgtcttttt attttttcca
      241 gctttcgact aattcttggt tctctttgat ccagtctttt gctgcttgct gtggatcttt
      301 tccattgttg atttccaaca taactttttc catatctttt tctgaccaat ggaaattgtc
      361 taatactttg taagcttctg gctggtcttc tttcaatcct tttcgaacca tcgtatggat
      421 ctgctcttcg cttcccattc cgttttcagg atcttctaag tatttcagat catatttggc
      481 aaacatccaa tgaggtgtcc atcctgtaac tacaattggt tcgtgttttt tgatcgcttg
      541 acctagtgca acggtcattg ctcctgaaga agatgtctct actttccagt ctttcaagtt
      601 gtcatatttt tggatcgtgt tttcagcagc cgtaactaca ccagctcctg gctcgattcc
      661 tatgattttc ttacctgcct gatcggttaa ctcatcgatc gaatttacat ccatgtaggc
      721 tggcactgct aaaccaactt ttgctcctgt caaattggca cctaggtctt cgacttgatc
      781 tttatattgt gcatattgtg aaccatgagt tttaggtagc caagctgaaa ccatggcatc
      841 tgactcgcca tttgaaacag atttccacat gattgaatta tctaatggcg tcatcgttac
      901 atcatagccc atttgtttca atacttcacc gactacattt gttgaagcaa cttctgtatc
      961 ccattcaaca taagataagt tgatacgttt tgtttctttg gcagatgaaa aactccctat
     1021 accaatcgca cctaagatca ctactactac agcaccaatg atcagtcctt gacgtttttt
     1081 acttttctgt gttgttgctc ctgcagcttt tttattgttt ggctggttca aatgctgtgt
     1141 aaaacggtca atgatgattg ctaagataac taatgcaacc ccgttgacaa atccattccc
     1201 gacttgtgct ctttgcaatg ctgataatac gccgcgtcct aagccgggtg caccgatcat
     1261 agaggcaata acgaccattg aaagtgacag cattgttgtt tgattgatcc ctgccatgat
     1321 cgtactttta gctaaaggca attctagttt gaatagtttt tgacgacttg tgctcccgaa
     1381 tgaatcagaa gcttctacta attctttagg aacttgtcga atccctaaat tcgtaaaacg
     1441 aacggttggt ggcagagcaa agatgactga ggcaaatacc cctggaacca tcccgatacc
     1501 gaaaaatgca actgctggaa tcaaataaac gaatcctggc attgtctgca taaagtcaag
     1561 gatcggtttg ataatacttt gggctttact gctttttgcc atcaagatcc ctaatggtac
     1621 cccaatgatg atcgagatca cactagataa aagaacaagt gtgacagtat tcatcaaatc
     1681 attccataga ccttggttat aaataaacca tagaccgatt aatgtgaaca acgtaagtcc
     1741 tggacgtttc ttagaaataa agaaagctgc taccgtcaat aacaagataa acaaaggagc
     1801 tggaatcact aacagtaaat ttgtaatccc cgacatcact gcctgtccaa tcgtttgtaa
     1861 aaatgaaaat aggccagaaa aagttgtcgt aaaccattct gtgatatttt ctacccagtt
     1921 tgccactggt aattgatagt tcaatagatt attcatgttc actcacctcg cttgtttctg
     1981 ctaatgcttc aatcacacta cctcggatga cgacaccttt taattttcca tcctctacaa
     2041 ctgcaagtgg agtcggtgag tcataaataa tattgaaaat atcacttacc accatgtctt
     2101 tcccaatcac cgtcacattt tcatctagga agtcgatcaa tggacgtttt tgcttcctag
     2161 cttctaacgc ttgatctgca gtgatgatcc ctttcaggtg tctctttctg tcaacagcca
     2221 tcagcatgct gacttcttca ttacgcatcc ttgtcaatgc aacgtttggt ccatcagatt
     2281 cgatattcgt cgtcaatgca ggtaccatga tgttttgagc cgtaagcact ttggaacgat
     2341 cgacttcttc cacaaattca cgtacataat catttgctgg attggtcaat atttcttcac
     2401 ctgttccgat ctgcatgatt tcgccgtctt tcatcaaggc gattctgtcc ccaatccgta
     2461 aagcttcatt caaatcatgg gtaatgaaaa taatcgtttt ttgaacattg gcttgcaggt
     2521 ccagtaattc gtcttgcatt tctcgccgga tcagcggatc aagagccgag aacgcttcgt
     2581 ccatcagcaa gatctcagga tcatttgcta aagctctggc taggccaacc cgttgttgca
     2641 tcccaccaga taactgacta ggatattggt ctttaaatga aagcaaacta gaattttcta
     2701 atgctttttc tgctttttct tgtcgttctt ctttaggtac accacggact tctaacccgt
     2761 attctgtatt ttccaagatc gttcgatgtg gaaacagacc gaagttttgg aaaaccatat
     2821 tgattttatg acgacggact tctcgcaatt cttctttgct taacttcgat acatcttctc
     2881 catcaatata gatactgccc gaagttggct caatcagacg attcaataga cggatcaatg
     2941 tggatttccc actcccggaa agccccatga tcacgaagat ttctccttct tttacgtcaa
     3001 aattcacgtc atacacacca accgttgcac cggttttctt taaaatctcc gttttcggct
     3061 ggtgatcatt catcatatct aaagcggctt gtgtcttttt accaaatatc tttgttaaat
     3121 gttttacttc tacttttacc aaacaaattc tcctttttta tgtaaattca tttttctttc
     3181 tgaaaaaagt gaccacacta tgttaacatc atgtagtcac tttgtcaaaa atgaaagaaa
     3241 tttgcttgtc caaactgaaa aaaacttggc taaatctttg taaataaaag gtttagccaa
     3301 gttaatattt ttttaatatt tcacacaatc attctgcaag ctgatgctcc gggtaaaaaa
     3361 agtttgtcat acgctgtagt gtatattcat ttccaataaa atgaacgatg tcccctgccg
     3421 taaatttagc ataggggcca ggtgaaacaa gcagttcttc attatgagaa attgcgataa
     3481 ctgtggcgcc agtcgcctgc cagatattca actcgctaat tgaacggtcc agatgcattg
     3541 ctttttctga taaagataat tcaaacggaa gtaaaggatt catcttatgt attcgcttcg
     3601 tttgattgac cagctgatcc aataattgag aaaaatgatt cagctcatct tgttgcttct
     3661 ccacacttgc catgatttct gcttttgtat cttggatgga ttgtacatct tgatattgca
     3721 ataggaaatc ttttgctttt tccttcgaag caacaaatgc cccacttcca tgccgtactt
     3781 ccatgattcc aagatctacc agcacattga tagccttgcg ggtagtctca ggagaagcat
     3841 tgtacgtact ggcaagtgtt gagcgggcat ggatcttctc acccacttta tatcggtttt
     3901 caacgatacg ttcggctaaa tcaacagcaa tctgctggta gcgcgggcgg acgatttttt
     3961 tctttccaga cattttcttt cctctttctt tttttacttt ccctcatctt actatgctct
     4021 agcaaaataa gcaaaaaagg ggagaatagc ctcttgcaac gactgatcat cgcttgatct
     4081 gttccttttt tactgggcaa gctgtttgac agtcatcaca attcttaccg gattttaaac
     4141 gagtatagac aattgcgcct gcacttccaa agattaacac acttaataag aatgtcgcca
     4201 tcctattcct ctccttctaa tgacaatata gtataaaact ggttcttgga ttttggctga
     4261 cggaacaaaa cataaacgag tcctatcatc agtaaaatag caagatacgt ccctaacgct
     4321 acctgtccac cctccaataa gtgtcccaac tgataaatca caaaactaac taaataagct
     4381 aaaccgcatt gatagcctat ggcgatccaa gtccatttct tggcattcat ttcccgatgg
     4441 atagccccaa ttgccgcaaa acaaggcgca caaagtaaat tgaaggctaa caatgaataa
     4501 gctgctgcgg gagtatatgt gtgttgtagc acttgccaga tttctctgcc attttcggat
     4561 acttccgcat gaccaaacaa aataccaaat gtgccaataa tattttcttt agcgaccaat
     4621 cctgtaatcg ctgcgactgt tccttgccag tttccccaac caagcggctg aaaaacaact
     4681 gccaggattc gcccgaaaaa ggctaagata ctttggtctt cagaaacagg ttgagcataa
     4741 aaattataag aagagctgaa ccagatcacg atactagaaa caaagataat cgttcctgct
     4801 tttttaacaa aagacttgct gcggtcatag gtttgtctta ggaccgctga ccattgagga
     4861 aaatgatatg cgggcaattc catgataaaa ggagctgtat ctccagcaaa caaacgtgtt
     4921 tttttcaagc tgattccaga gaaaataatt gcagtcatac caagaaaata ggcagaagga
     4981 gctacccaag attgatgagg aaaaaaggcc cctgctacta aggcaataat cggcaacttt
     5041 gcagaacaag gcatgaaggt tgtgaccatg atcgtcaaac gacgatcttg ctcgttttca
     5101 atcgtgcgac tggccatgac gcctggcact ccacatcctg tagcaatcag cataggaatg
     5161 aaggattttc ctgaaagacc gaatttcctg aaaagtcgat ccatgacaaa agcgatcctc
     5221 gccatatatc cgcagtcttc cagaaagctc aagcataaaa acaatataat caattgcggt
     5281 agaaatccta acactgctcc cacgcctgcg atgatgccgt tcaagatcag ctcctgcatc
     5341 catcccgcta cttgccattc agctaacaat ctgccaacat gttctggtaa ccatgtacca
     5401 aacaatgtgt cattgatcca atctgtcccc attgttccta ctgtttgaat agccagataa
     5461 tagatcagcc acatcacaaa tgcgaagatc ggcaaggcta accaacgatt cgttgcaatc
     5521 tgatcgattt tgtcactcat cgataattgg aaagaatcat ttgacacaac acagagtgta
     5581 catagttggg taataaattc ataacgttca ttgaccagaa tcgtctcact atcgtcgtcc
     5641 aatagtttct ctgttaactg gatcagttgc tcgatctctt tttgctgaga aactgctatc
     5701 tcgtaatcct ctcttacctt ctgatctcgt tcaaaatatt tcattgcata ccagcgtttc
     5761 ttgccgcttt gttctcctag tacatcttca atttcatgta aagctgcttc taaacgtgga
     5821 tcatatgtac agggaacagg aatagaaggc cgcttttttg cagactcctg aatcttttcg
     5881 attagttttt cgattccttg ctttttcaat acgctgattg gtaaaactgg tacaccaaga
     5941 ccataggaaa gcttttctag attgattttt tttccttctt ttttgatgac atccatcata
     6001 ttcagaccaa tgatcatagg aatgtctaaa tccaataact gcgtagttaa ataaagattt
     6061 cgttctaaat tggtagcatc aataatgttg atgatcagtt caggctgttc ctgaaccaaa
     6121 taatctcttg caatcacttc ttctggtgta taaggagaca aagaatagat gccgggtagg
     6181 tcctgaatat gaatgttcgg attttttttc aacgatcctt ctttttttct actgttacgc
     6241 ccggccaatt ccctacatat tgattcgaac cagtcaagag attgaacaag ctagttttcc
     6301 cgctgtttgg atttccgact aacgcgatat tcattctttt tcctcccttt gtatcaagat
     6361 tagttcggcc tctgactttc gtaacgtcag ttcataccct cgcactgtca gttcgattgg
     6421 atctcccaga ggggctagtt ttcggataac tatttttaca tttcgcgtca atcccatatc
     6481 cattaaccgt cgcttcactg cgccttgtcc gtggattgct acgacgatcc cagtctctcc
     6541 tgcttttaat tgatcaagag aagtccagct ttcagaggaa gcagtctttt tattgacaaa
     6601 gatttgacct agtatttccc gacttactgc cactcgatga ctttttacca gtaaaatggc
     6661 attgttgcct gataaattga cgacgacgat tttacttccg gcaacgatcc cgatattttg
     6721 caaatggcgt tttgtttttt cttctgtttc gattcgttcg acttgataaa cgaaattctg
     6781 ctttgcctgc gctaaggtct gcatattgat tcctctcttg tgaaataaat catgtattaa
     6841 aatcattcat tatatgttct aattttctta cgtgttacgc aaataaatga gaatcattct
     6901 cacatagaat actctcggta attgagaatg tcaattatac ttgtgatatt atgagcgaat
     6961 ctttagtatt gatagataaa aatgagaaaa aaatatttct tcatagtttt ccacaatttt
     7021 ttctctatta aatttgctat tttcccacac gatacttgtt ttcagctctt ttttcaaaaa
     7081 cacaaaactt agtattccac agtttccaca acacaatatg tagtgtttgt tgtttgactt
     7141 ttttactaag agttttatac tatctattat agataataag aatgtgttag aggaggcact
     7201 caccatgaac gtaaaaatct tttctaaaaa taattgtatg caatgtaaga tggtcaaacg
     7261 gtttttaaca gaaaatcaaa ttgcttttga agaagtcaat atcgatgaac agcctgaagc
     7321 agtagactgg ttgaaagcac aaggattcca aagtgttcct gtcattactt ctgatgcaga
     7381 aacagttatc ggattccgtc ctgatcagtt gcgtaaactt gccagctaac atgactaaaa
     7441 gaatcaaaac gtgatacatg gatttgaagg gcttgagaca gcaccagttt ttgtcgcaaa
     7501 cttccaaatc cttttttaaa gcttaaaaac aatgcaatga tttgcaatca agcatcatca
     7561 aatacgaaat gaaggttggg cgtacgtatg agtcttaaaa atttacaaga tgtcagttat
     7621 ttcaagctga ataatgaaat caatcggcca gtcaatggtc aaatcccttt gaataaggat
     7681 aaagaagctt tagttgcctt ttttgaggaa aatgtcaaac caaatacgat gacttttcca
     7741 tctattatgg aaaaaatcaa ttacttgatc gatcaagaat atatagaagc agaattcatt
     7801 caattgtatt caccatcatt tattgaaaag ctatatcaat ttttggacga gcaacatttt
     7861 tctttcaaat cttttatggc tgcttacaaa ttttattctc aatatgcgtt gaaaaccaac
     7921 gacggttccg cttatctgga aacttatgaa gatcgagtag ccttcaatgc gctttacttt
     7981 gcgaacgggg atgaagaatt agcacttgct cttgcagacg aaatgatcca tcaacgatat
     8041 cagccagcta caccttcttt cttgaatgct ggacgtaaac gtcggggcga attggtgtct
     8101 tgtttcttga ctcaagtcac tgacgatatg aactctatcg ggcgctcaat caactctgct
     8161 ttgcaattgt cccgtattgg tggcggcgta gggattactt tatcaaatct tcgtgaagcc
     8221 ggcgctccta tcaaaggata tgagggtgct gcaagtggtg tcgttccagt catgaaatta
     8281 ttcgaagata gtttcagcta ttctaatcaa ttaggtcagc gtcaaggagc tggcgtggtt
     8341 tatttgaacg ttttccatcc agacattatt gctttccttt cagcaaaaaa agaaaatgca
     8401 gatgaaaaga ttcgcgtaaa aactctttct cttggcgtga tcgtacctga taaattttat
     8461 gaattgacac gtaaaaacga agatatgtat ttatttagtc catatagtgt cgaaaaagaa
     8521 tatggtgtcc cattctctta tgtaaatatt acagctgagt atgatcatct agttgcaaat
     8581 ccaaacattc gcaaacaaaa aatcaaagcg cgtgatcttg aaaatgaaat ctcaaaatta
     8641 caacaagaat ctggctatcc ttacgtaatc aatattgata cagcgaaccg cgagaatcca
     8701 atcgatggca agatcatcat gagtaattta tgttcagaga ttttacaagt tcaaacgcct
     8761 tctgtgatta acggcaaaca ggaatatgaa gtattaggaa cagatatctc atgtaactta
     8821 ggctcgacaa atatcgtgaa tttgatgcaa agccctaact ttggtcaatc tgtgcgtacg
     8881 atgactcgtg cattgacatt tattacagat gcatctgata tcgatgtcgt accaccgatc
     8941 caaaacggaa ataagctgaa tcatacgatt ggattaggtg ccatgggctt acacacttat
     9001 cttgcaaaag aacaaatgga atatggctca gaggattcat tagactttac aaatatttac
     9061 ttcatgctgt tgaattattg gacacttgtt gaaagcaaca atatcgctcg tgaaagaaaa
     9121 caagttttcc ataattttga aaaatcagct tatgctgacg gcacttattt cgaaaaatac
     9181 gtaactggag aattccagcc aaaatcagaa aaagtcaaag cattgtttga aggcattttt
     9241 atcccaactg ctgaagattg gaatgctttg aaacaagcag taatgaaaga tggactgtat
     9301 catcaaaatc gcttggcagt agcaccaaat ggctcaattt catatatcaa cgatacgagt
     9361 gcaagtattc atcctatcac tcgcatgatc gaagaacgcc aagagaagaa aatcggtaaa
     9421 atttattacc cagcgcctta tctatccaat gagacgattc cgtattacac ttctgcttat
     9481 gatatggaca tgcgtaaagt aatcgatgtt tatgcaacgg cacaacaaca cgtcgatcaa
     9541 ggcatgagtt tgactttatt catgcgttct gaaatcccag aaggcattta tgaatggaag
     9601 acaacaacga agcaaacgac acgtgatttg aatattttaa gacattacgc tttccacaaa
     9661 ggaatcaaat ccatttatta cgtacgtacc tttactgatg atgcagaaga aatcggaagc
     9721 aatcaatgtg aaagctgcgt gatctaatat catagataga ttcgcttatt catattttaa
     9781 ttaaaggagc attctcatgt cagaaacata ttatgaagca ataaactgga atgaaattga
     9841 agatatcatt gataaatcca cttgggagaa gctgacagag caattttggc tagatacacg
     9901 tatcccactt tcaaatgacc tagatgattg gcgtacattg tctcaattgg aaaaagatac
     9961 agtaggacat gtatttggcg gtttgacact gctagatact gtccaatctg aaagtggcat
    10021 ggatcagttg cgtaaagatg tccgcactcc ccatgaagaa gccgttttga acaatattca
    10081 attcatggaa tccgttcatg ccaaaagtta ttcttctatt tttagtacac tgaatacgaa
    10141 aaaagaaatc gaagaaattt ttgactggac aaatacgaat ccttatctgc aaaaaaaagc
    10201 tgagcgtatc aatgaaatct acaaacacgg tacaccgcta gaaaagaaaa ttgccagcgt
    10261 ctttttagaa acattcttgt tctattcagg tttttatact cctctttatt atttaggaaa
    10321 caacaaattg gctaacgtag cagagatcat caaactaatc atccgagatg aatctgtaca
    10381 tggaacctac atcggttaca aattccaatt aggattcaac gagttacctg aagcagaaca
    10441 agaaacgtta aaagactgga tgtacaatct tctttatgag ctttacgaaa acgaagaacg
    10501 ttatacagaa gaattatacg atccaatcgg ctggacagaa gaagtcaaga cattcctacg
    10561 ctataatgcc aacaaagcat tgatgaacct agggatggat ccactctttc cagatactgc
    10621 caatgatgtc aacccaatcg taatgaacgg gatttcaaca ggcacaagca accatgactt
    10681 cttctcacaa gtcggaaacg gctatttgtt aggtcatgta gaagcgatga aagacgacga
    10741 ttatttgatt ggattggaat aaggatttat tttaattcaa aaatttttcg aaaaataaaa
    10801 agagtttagc gtacagatat tttaaatgta cactgaactc ttttttatgc ttattcactt
    10861 gtttcttcca gcacttttgg actgtctaac ttttccgaaa catgttccat tcttctcaat
    10921 aaatagattt tccggatatt taatattatc gtcttaatca ctgcatagac aggtatgcaa
    10981 atcagcatgc ctaatattcc tgcgacactt cctgctccaa tcaataccaa gatgatcgtc
    11041 aaaggatgga tattcattga tttcccaaat aacaacggct tgatcaaatt tccatctaat
    11101 tgttggatca ctaaaatcga caacgccata tacaaagctt gaaatggaga gataaacaaa
    11161 ccgacaatga ttgctggtac cgctccaata aaagggccga catacgggat aatattcgtg
    11221 attccgcaaa tgaccgcaag caacaagcta taaggctgat cgaatattct cattgccaaa
    11281 aagctcatta caccaataat caaagcatct aagatcgtac tgctgatata ggcagataat
    11341 gtttcgttta attcttttac cgtttggcgt aactctttac gaatcgcttt cggaaagaag
    11401 tgagacaacg catcaataaa tttgtgccca tccttgaaca taaatagcaa aataaatgga
    11461 acggtaaata acaaaataaa aaattgcatc ataaatgaaa tgatttttga gacacttgca
    11521 gataaactga ctaatatgat attgaacaaa ttactaagag ataaatttgc tgatgctaat
    11581 tgttcttcca aattgaaatt tttaaactct tcccgctgcg ccaattcatt taaccatttg
    11641 cttgtttcat ctgcatacat tggcaagctc tgggtcaatt gaattgtctg ttccattaac
    11701 tgaggcacta cattcatgac agttaaaaca actaaaatga ttacgaccaa aaacgtcaat
    11761 aaaaaaccga acactctcgg gatccgatgc ttttcgagaa aaacaacgat tggatcgaac
    11821 atataataga taaaaccagc aacaagcaat ggtaaaaaaa ttgccgaaag cattttcaat
    11881 attggactaa aaatattcgg catttgtaaa ataaagtaca cgccgatgat cgtgagtact
    11941 agctccactg tccagaaaaa taattttgat tgtttaaaac gtgattgcat acatctcctc
    12001 caaggtcctt tgatacttct attctatcat ttaacaaaga gttacgcgct gagattttca
    12061 tttgcttaaa aaaaacaaaa aaaataaaca tattttaatg agtagacgag tacttaaaca
    12121 aaaacacggt ttggagtagt gacaaaaatt atactgcagt ccctttttcc aaataaactg
    12181 tgttcaaaag cttgcatctt cgtaataagc ccagccaaac gaaaaatata aggaactatt
    12241 tttatttggc tgggcttatt acttgatgta ggacatctat tttacaacct cagatcattt
    12301 attgcgcaat actgactaaa tgcaactttg tgatattttt gtgtacggac aactcctcga
    12361 tcacatcggc ataagtgagc ccttttggaa gatcaatcgt atagatattt ttgtacactc
    12421 ggtagtcatc aacgaatttt acgtcaaaat tcacatcttc gattacgata tttttctctt
    12481 caaaataatc ggtaagaaaa tctttggttt cttttctatg gataaattgg atttctaact
    12541 ttttagtcgt aggtacatgg atcacgcgtt tcactactgt gagtgcaata gcaatcccga
    12601 taaaactgat gatcgcaatg gaatagtaac ccatgccgat ggcgatccct aatccagcaa
    12661 ttgcccataa agaagcggca gtcgtaagtc ccgtcaccga ttgtttcgtg acgatgatcg
    12721 ttcccgcgcc taagaaaccg acaccactca ccacttgagc aatcagcctt gcttcatctg
    12781 aacgaatcgt tccagcaagc tgtggatact ctaaagcatc tcttaatgca ccactagcga
    12841 tctcaacttg gatcaatgca atgatacatg ctcccataca aactaaaata tgcgtacgca
    12901 tacctgctgg acgattttta tattgtcgtt caaaaccgat tgctccgcca aatagcatag
    12961 cgatccctaa acgtaaaata atctctggaa ttgtcaatac catatcattc atctaaacac
    13021 cctccagtcc aatatatcat gtctgaaagg agcagacaaa aaaagtgcaa tttttacctc
    13081 ttacgaaaaa aggactgtga caaaattttt gtcatagtcc tttttatgaa taaacggtgt
    13141 tcaatcagct gaccctcggt attactcaac aaacggcaaa atatggggag ctatttttcc
    13201 tttgtcttct ctaatactca ggtcaaaacg ctgatttcac aacctcttta gcctactatt
    13261 gaaaattaca acttacttgc tgcttcttca tcgatgatca caacaacatt gtcgtgattt
    13321 tgcaatacac ttgctggcaa atgattcgtt acaggtcctt caatcatccc tttgattgca
    13381 tcagctttgc tttcgccaaa tgccatcaag acgatttctt tacttttcat aattgaaccg
    13441 attcccattg aaacagcttt tgttggtaca tcttctgctt tatcgaagaa tcgtttgttt
    13501 gcattgattg ttgattctgt taagtcaact actgaagtcg ttccgtcaaa tgaagcacca
    13561 ggttcattga aaccgatatg tccattttgc ccgattccta agatttggat atcgattgga
    13621 tgtgcatcga tgatttgatc atactttttg catgctgctt ccagatcttc tgcttttcca
    13681 tctggtacaa acgtttcttt gaatggtttt ttatcaaata attggacatc catgaaatga
    13741 cggtagcttt gttcatctga accgcctagt ccaacgtact catctaagtt tacagaagtc
    13801 atttcactga aatccaaatc gcttgaagtc atttctttat ataaagtaac tggtgtgctt
    13861 cctgttgcca atcctaatac ttttgcacct ttgttcatac cttctttgat gatttcaaat
    13921 gctttttttc cgccttcttc agcgtttttt acacgaatga tttccatagt gattgctcct
    13981 ttatattttg gtatagccca attatagcat tctttttttc tttttacaat aattagtata
    14041 gaccaaattg catttttttc attttcttga caataaatag gaatccgtgt taggtttcat
    14101 aagatggttc aatcaaacaa tccatgttgc atgaaaacca agcaagtgat tcactgtgga
    14161 cgctttatgt ctgcagtttt tttatgaaaa aggagttatt atgagtatac tgacattaaa
    14221 caacattacc cagcaatttg gcgacaaaat cctctatgaa ggtgtcgatc tgcaattaaa
    14281 tgctggcgaa catcttgggc tgattggaca aaacggagcc ggtaaaagta cattgattaa
    14341 aatcatcact ggtgagatct taccagatga cggacagatc cattggcaaa aaaatattca
    14401 caaaggttac ctcgatcaat atgtggaagt cgatgaaaca ttaacaatcg aagaatttct
    14461 aaaaactgcg tacgcaaaag aatacgaaaa ggaagaaaaa attgcttttc tttaccaaac
    14521 atatagcgag accttcgatg attccttgct ggaaaaagct gggaaactcc aaacggaatt
    14581 agaccaagga gtgttttatc aaatcgacac cttagttgct gagatgagca gcgggttggg
    14641 gatcgatgtc ttaggtttag attcactgtt gaaaaatcta agtggtggac aacgttcaaa
    14701 ggtgatttta gcaaaactac ttttagaaaa cccagatgtt ctgatcttag atgaaccaac
    14761 caatcactta gatgatcagc atatccaatg gctgacgcag tttcttcagg attttgacgg
    14821 tgcgtatatc gtgatttccc atgaccaaga atttcttgat cagatcacca cccatatcgc
    14881 agacatcgag tttggaaaga ttactaagta tactggccat ctaaaacaag cattgaaaca
    14941 aaaggaacaa aatcgcgaaa gctatttgcg ccaatatcat gctcaacaaa aacaaatcga
    15001 aaagaccgaa gcttatattc gaaaatacaa agccggttcc cgatcaacga tggcaaaaag
    15061 ccgacaaaaa caattagata aaatcgaacg gctgacgcct ccagcttcag caagtaaagc
    15121 tgtctttgat tttccattct cgcctattgt cacgacatta gcagtggaaa caactgattt
    15181 agtcatcgga tatgatcgac cgctacttgc acccatcaat ttgaccatcc gctttggtga
    15241 aaatattgcc attcgaggct tcaacgggat tggaaaatct actttattaa aaacactgat
    15301 cggtgagatt gaaaaaataa gtggcgattt ccacttccca gataacacaa aaatcaatta
    15361 cttttcacaa gatctgtact gggaaaatcc attagaaaca ccactacaat atttaagcag
    15421 tcaatttcca aaagcgacta tcaaagaatt acgtcgccaa ctagctaaag cagggttagt
    15481 taatcaatta gcaagtgaac ctctcactac attaagtggt ggggaacaaa ctaaggtgaa
    15541 acttgcacag ttgacaatga accaaggtaa tttactgatt ctcgatgaac cgacgaacca
    15601 tatcgatcaa gaaacgaaag aaagcttaca agacgggata cagaaatttc caggaacagt
    15661 aatcatcgtc tctcatgaac aagaatttta tcaagatctt gtgcaacgag tcattgagat
    15721 tgaaccaaaa taaacgtttg attgaatagt tgaaaaaaag gagctgtgac aaaagttttg
    15781 tcacaactcc ttcttctgaa taaatggtgg ccaattagct gaccttcggt attagctcaa
    15841 aaaacggcaa aatatgagga gctattttta tttggctatt cttattactt gatgcagaac
    15901 agcttttcac aacctctttt cgtctatttt ttattttcga gatttctctt tcctttaaaa
    15961 acaactgttt gaaagaagaa cgcacatcct tgaaaaacaa gcccgctaac cgttacacct
    16021 aaccagccat actgctgcca cattgtcgtc cctagcaatg aaccagccgc acctccaata
    16081 aagtacataa acataaata
//
LOCUS       DAZABD010000060        16082 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000060 DAZABD010000000
VERSION     DAZABD010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16082
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.060"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            201..1169
                     /locus_tag="ACHALG_002118"
     CDS             201..1169
                     /locus_tag="ACHALG_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFN2587489.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1162..2193
                     /gene="vanA"
                     /locus_tag="ACHALG_002119"
     CDS             1162..2193
                     /gene="vanA"
                     /locus_tag="ACHALG_002119"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFN2587490.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2199..2807
                     /locus_tag="ACHALG_002120"
     CDS             2199..2807
                     /locus_tag="ACHALG_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HFN2587491.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
     gene            3235..4146
                     /gene="vanY"
                     /locus_tag="ACHALG_002121"
     CDS             3235..4146
                     /gene="vanY"
                     /locus_tag="ACHALG_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HFN2587492.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYELWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            4299..4784
                     /gene="vanZ-A"
                     /locus_tag="ACHALG_002122"
     CDS             4299..4784
                     /gene="vanZ-A"
                     /locus_tag="ACHALG_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HFN2587493.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(5071..5400)
                     /locus_tag="ACHALG_002123"
     CDS             complement(5071..5400)
                     /locus_tag="ACHALG_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HFN2587494.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
     gene            5705..6034
                     /locus_tag="ACHALG_002124"
     CDS             5705..6034
                     /locus_tag="ACHALG_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFN2587495.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            6024..6311
                     /locus_tag="ACHALG_002125"
     CDS             6024..6311
                     /locus_tag="ACHALG_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFN2587496.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KTGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPILLCGTHENFYEQLKKIR"
     gene            complement(6590..7153)
                     /locus_tag="ACHALG_002126"
     CDS             complement(6590..7153)
                     /locus_tag="ACHALG_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFN2587497.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAV
                     RPQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTP
                     HGRLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVDRAVKLYKSQ
                     VYSVKEITEMTGISKATLYRYLKDNRE"
     gene            7796..8749
                     /locus_tag="ACHALG_002127"
     CDS             7796..8749
                     /locus_tag="ACHALG_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFN2587498.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEIIFPAEEKEYIEKRSTEGWTPDVIIGRAERTFS
                     CSISTLYRRFKTGEFNVLHLPMQGKRKPNGYKEKRGKQAFKRNISERKKDYVVFEEEF
                     GHLEGDTIVGIHHKSAVITLVERLSKAIIVLKPEGRKAVDIENSINEWLQSVPKNLFK
                     SITFDCGKEFSNWKSISNTNDIDIYFADPGTPSQRGLNEHSNGLLRKDGLPKEMDFNQ
                     VNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA"
     gene            8727..10202
                     /locus_tag="ACHALG_002128"
     CDS             8727..10202
                     /locus_tag="ACHALG_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HFN2587499.1"
                     /translation="MESFVSLNLTNNTSKKNQPKDIKDIVDTRMYKERDDLNYTLIIN
                     KDGNYSWRPLVLLHPILYVDLVNCLTKEDHWEELKKRFSEFKKDKNINCYSIPVEASK
                     GSKRTDLGETILNWWEYFEQKSLAQNIKYQYCMFTDIADCYPSIYTHSITWAMYGKEK
                     VKNDENKCSGSLGDKIDKKISGMQHNQTNGIPQGSVLMDFVAEIVLGYVDLRLSKELK
                     KLENLKDYKVLRYRDDYRIFSNNRNDLEQIMKVLSEVLYDLNFKLNSSKTKLSDDIII
                     DSIKKDKLYWEEIKASIRMNATNLDGDREIRYSINLQKHLLEIKKLADKHPNSGQLKK
                     ALSEFNKERIIKITSKPNDLLPLIGILVDIMINNPGSLPQCVLIIGQLLNFENESKAR
                     EIVEMIINKYALKPNTEFMMIWFQRLTTIFGEMFDSVLSSEFVSKIKDPVSNQMFSVD
                     WLNEDAKKSFYEGTIKDEEKYIKMSKKIYITEFDDFNDYPF"
     gene            10540..12162
                     /locus_tag="ACHALG_002129"
     CDS             10540..12162
                     /locus_tag="ACHALG_002129"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFN2587500.1"
                     /translation="MTEQIEKNSKSLYQALWNSADILRSKMDANEYKSYLLGLVFYKY
                     LSDNMLRYVSVLLEEETDDLNKAQDLYVEAFKDADIKDDLLYELKDEFSYTIAPALTF
                     TAQVAAIHDGSFQLEDLVQGFRDIEQSSENFENLFEDIDLYSKKLGSTPQKQNKTIAD
                     VMKELEGLNMAGHAGDVLGDAYEYLIGQFASESGKKAGEFYTPQPVAKLMTQIVLQGK
                     EDKKGFSVYDATMGSGSLLLNAKKYSHQPGTISYFGQELNTSTYNLARMNMILHGVPI
                     ANQHLHNADTLDQDWPTEEPTNFDGVLMNPPYSAKWSADKGFLDDPRFSAYGVLAPKS
                     KADFAFLLHGYYHLKDTGVMAIVLPHGVLFRGGAEGKIRKALLENGAIDTVIGLPANI
                     FFNTSIPTTVIILKKDRAKKDVLFIDASQDFEKIKTQNTLRDYHIDAILEAYKTRTIK
                     DKYAYVAEFDEIVENDYNLNIPRYVDTFEEEEVIPLDSVSKSIQETKAELAQAETELF
                     NMLKELNGTTEEAENELQAFISQLMTDGDNNE"
     gene            12155..13408
                     /locus_tag="ACHALG_002130"
     CDS             12155..13408
                     /locus_tag="ACHALG_002130"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002402613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFN2587501.1"
                     /translation="MNKERAIPKVRFKSFSGDWKKLKLSSVTSRVRGNDGRMSLPTLT
                     ISARNGWLDQRERFSGNIAGKEQKNYTLLRKGELSYNKGNSKLAKYGVVFMLDNFEEA
                     LVPRVYHSFKTTNEASSKYIEYLFETKKPNKELRKLITSGARMDGLLNINYDDFMGIK
                     ITIPKIKEQKKIGSLFKQIDGTITLQQQLLTDYKQFKKALLQQLFPQKGESVPKIRFT
                     GFSDDWELKELKEFIGEDVSDGDWIQKEHIHESGEYRIVQTGNIGIGRYIDKPESAKY
                     LNQESFDELKANEINPGDILISRLADPAGRALILPFTSSKMVTAVDVAIIRPNKNFIS
                     HFLVTRMNSSETLNDISKQVSGTSHKRLSRKNLEKIELNVPNIEEQEKIGQLFKKLDE
                     AIAGHEQKLATYQELKKALLQRMFV"
     gene            13430..15973
                     /locus_tag="ACHALG_002131"
     CDS             13430..15973
                     /locus_tag="ACHALG_002131"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HFN2587502.1"
                     /translation="MAITFTSESELEQTLIHQLIGGESQWTYRGDLNTEDKLWDNLRE
                     KLERNNKDVLNDVHLTEQEFHQVKNQLQFANFYQASKWLAGENGIAKVQVQREDASLG
                     TIRLKVLNRADIAGGMSSYEVINQFQSFKQSTDDRDRRFDVTLLINGLPLIHIELKNR
                     AHSYMDAFRQIKKYLVQGKFTGVFSALQMFVVSNGSDTRYIAAAQDTRLNEQFLATWV
                     DANNDPVTSYLAFAQHVLSIPQAHKMVTQYTVIDSDKKALLLLRPYQIHAIEAIKQAS
                     IRQESGYVWHTTGSGKTLTSYKAARNLLHIPSLDKTIFIVDRVDLDQQTTSSFASYAE
                     NDVIQIDETDNVKDLIKKLLSADRTVIVTTIQKLNHLMRRIEGKETTPRYKKLRALNV
                     AFVVDECHRAVSPQKKQEIDRFFRQPLWYGFTGTPIFAENAKKETGNLARTTEEQYGS
                     RLHEYTVKEAIHDKAVLGFQIEYKSTFSDDELDDLVLQVDPDADVRALTVLEKEAIIP
                     KSVYEHEQHMLEVIHSILNKSRRKLGFQNGSGKTYTALLTTSSIAQAQAYYDLFKRVV
                     AGETALDIHEKVKSVLPDFPKIAMTYSLSGNEEASVSHQAKMAEALADYNSLFDTNFS
                     IETMRAYNRNVNDRLARKQEKYLFRKEQLDLVIVVDRLLTGFDAPCLSTLFIDRPVMQ
                     PHNVIQAFSRTNRLFDRSKQFGQVVTFQTPALFEEKVSEALFLYSNGGENAVLAPTFE
                     EAHVAFKEAVAALRDVAVMPDAVDSLTDAQKKRFAKAFQRLDKTFAAIQVYSEFEEAD
                     LERDYQICGEEIAHYHGKYVNVLEEIKENMDSEQPEPLVIDIAGSVAKF"
BASE COUNT         5482 a         2755 c         3176 g         4669 t
ORIGIN      
        1 tttttaggaa aatctcaagg ttatctttac tttttcttag gaaattaaca atttaatatt
       61 aagaaacggc tcgttcttac acggtagact taataccgta agaacgagcc gttttcgttc
      121 ttcagagaaa gatttgacaa gattaccatt ggcatccccg ttttatttgg tgcctttcac
      181 agaaagggtt ggtcttaatt atgaataaca tcggcattac tgtttatgga tgtgagcagg
      241 atgaggcaga tgcattccat gctctttcgc ctcgctttgg cgttatggca acgataatta
      301 acgccaacgt gtcggaatcc aacgccaaat ccgcgccttt caatcaatgt atcagtgtgg
      361 gacataaatc agagatttcc gcctctattc ttcttgcgct gaagagagcc ggtgtgaaat
      421 atatttctac ccgaagcatc ggctgcaatc atatagatac aactgctgct aagagaatgg
      481 gcatcactgt cgacaatgtg gcgtactcgc cggatagcgt tgccgattat actatgatgc
      541 taattcttat ggcagtacgc aacgtaaaat cgattgtgcg ctctgtggaa aaacatgatt
      601 tcaggttgga cagcgaccgt ggcaaggtac tcagcgacat gacagttggt gtggtgggaa
      661 cgggccagat aggcaaagcg gttattgaac ggctgcgagg atttggatgt aaagtgttgg
      721 cttatagtcg cagccgaagt atagaggtaa actatgtacc gtttgatgag ttgctgcaaa
      781 atagcgatat cgttacgctt catgtgccgc tcaatacgga tacgcactat attatcagcc
      841 acgaacaaat acagagaatg aagcaaggag catttcttat caatactggg cgcggtccac
      901 ttgtagatac ctatgagttg gttaaagcat tagaaaacgg gaaactgggc ggtgccgcat
      961 tggatgtatt ggaaggagag gaagagtttt tctactctga ttgcacccaa aaaccaattg
     1021 ataatcaatt tttacttaaa cttcaaagaa tgcctaacgt gataatcaca ccgcatacgg
     1081 cctattatac cgagcaagcg ttgcgtgata ccgttgaaaa aaccattaaa aactgtttgg
     1141 attttgaaag gagacaggag catgaataga ataaaagttg caatactgtt tgggggttgc
     1201 tcagaggagc atgacgtatc ggtaaaatct gcaatagaga tagccgctaa cattaataaa
     1261 gaaaaatacg agccgttata cattggaatt acgaaatctg gtgtatggaa aatgtgcgaa
     1321 aaaccttgcg cggaatggga aaacgacaat tgctattcag ctgtactctc gccggataaa
     1381 aaaatgcacg gattacttgt taaaaagaac catgaatatg aaatcaacca tgttgatgta
     1441 gcattttcag ctttgcatgg caagtcaggt gaagatggat ccatacaagg tctgtttgaa
     1501 ttgtccggta tcccttttgt aggctgcgat attcaaagct cagcaatttg tatggacaaa
     1561 tcgttgacat acatcgttgc gaaaaatgct gggatagcta ctcccgcctt ttgggttatt
     1621 aataaagatg ataggccggt ggcagctacg tttacctatc ctgtttttgt taagccggcg
     1681 cgttcaggct catccttcgg tgtgaaaaaa gtcaatagcg cggacgaatt ggactacgca
     1741 attgaatcgg caagacaata tgacagcaaa atcttaattg agcaggctgt ttcgggctgt
     1801 gaggtcggtt gtgcggtatt gggaaacagt gccgcgttag ctgttggcga ggtggaccaa
     1861 atcaggctgc agtacggaat ctttcgtatt catcaggaag tcgagccgga aaaaggctct
     1921 gaaaacgcag ttataaccgt tcccgcagac ctttcagcag aggagcgagg acggatacag
     1981 gaaacggcaa aaaaaatata taaagcgctc ggctgtagag gtctagcccg tgtggatatg
     2041 tttttacaag ataacggccg cattgtactg aacgaagtca atactctgcc cggtttcacg
     2101 tcatacagtc gttatccccg tatgatggcc gctgcaggta ttgcacttcc cgaactgatt
     2161 gaccgcttga tcgtattagc gttaaagggg tgataagcat ggaaatagga tttacttttt
     2221 tagatgaaat agtacacggt gttcgttggg acgctaaata tgccacttgg gataatttca
     2281 ccggaaaacc ggttgacggt tatgaagtaa atcgcattgt agggacatac gagttggctg
     2341 aatcgctttt gaaggcaaaa gaactggctg ctacccaagg gtacggattg cttctatggg
     2401 acggttaccg tcctaatcgt gctgtaaact gttttatgca atgggctgca cagccggaaa
     2461 ataacctgac aaaggaaagt tattatccca atattgaccg aactgagatg atttcaaaag
     2521 gatacgtggc ttcaaaatca agccatagcc gcggcagtgc cattgatctt acgctttatc
     2581 gattagacac gggtgagctt gtaccaatgg ggagccgatt tgattttatg gatgaacgct
     2641 ctcatcatgc ggcaaatgga atatcatgca atgaagcgca aaatcgcaga cgtttgcgct
     2701 ccatcatgga aaacagtggg tttgaagcat atagcctcga atggtggcac tatgtattaa
     2761 gagacgaacc ataccccaat agctattttg atttccccgt taaataaact tttaaccgtt
     2821 gcacggacaa actatataag ctaactcttt cggcaggaaa cccgacgtat gtaactggtt
     2881 cttagggaat ttatatatag tagatagtat tgaagatgta aggcagagcg atattgcggt
     2941 cattatctgc gtgcgctgcg gcaagatagc ctgataataa gactgatcgc atagaggggt
     3001 ggtatttcac accgcccatt gtcaacaggc agttcagcct cgttaaattc agcatgggta
     3061 tcacttatga aaattcatct acattggtga taatagtaaa tccagtaggg cgaaataatt
     3121 gactgtaatt tacggggcaa aacggcacaa tctcaaacga gattgtgccg tttaagggga
     3181 agattctaga aatatttcat acttccaact atatagttaa ggaggagact gaaaatgaag
     3241 aagttgtttt ttttattgtt attgttattc ttaatatact taggttatga ctacgttaat
     3301 gaagcactgt tttctcagga aaaagtcgaa tttcaaaatt atgatcaaaa tcccaaagaa
     3361 catttagaaa atagtgggac ttctgaaaat acccaagaga aaacaattac agaagaacag
     3421 gtttatcaag gaaatctgct attaatcaat agtaaatatc ctgttcgcca agaaagtgtg
     3481 aagtcagata tcgtgaattt atctaaacat gacgaattaa taaatggata cgggttgctt
     3541 gatagtaata tttatatgtc aaaagaaata gcacaaaaat tttcagagat ggtcaatgat
     3601 gctgtaaagg gtggcgttag tcattttatt attaatagtg gctatcgaga ctttgatgag
     3661 caaagtgtgc tttaccaaga aatgggggct gagtatgcct taccagcagg ttatagtgag
     3721 cataattcag gtttatcact agatgtagga tcaagcttga cgaaaatgga acgagcccct
     3781 gaaggaaagt ggatagaaga aaatgcttgg aaatacgggt tcattttacg ttatccagag
     3841 gacaaaacag agttaacagg aattcaatat gaactatggc atattcgcta tgttggttta
     3901 ccacatagtg cgattatgaa agaaaagaat ttcgttctcg aggaatatat ggattaccta
     3961 aaagaagaaa aaaccatttc tgttagtgta aatggggaaa aatatgagat cttttattat
     4021 cctgttacta aaaataccac cattcatgtg ccgactaatc ttcgttatga gatatcagga
     4081 aacaatatag acggtgtaat tgtgacagtg tttcccggat caacacatac taattcaagg
     4141 aggtaaggat ggcggaatga aaccaacgaa attaatgaac agcattattg tactagcact
     4201 tttggggtaa cgttagcttt ttaatttaaa acccacgtta actaggacat tgctatacta
     4261 atgatacaac ttaaacaaaa gaattagagg aaattatatt gggaaaaata ttatctagag
     4321 gattgctagc tttatattta gtgacactaa tctggttagt gttattcaaa ttacaataca
     4381 atattttatc agtatttaat tatcatcaaa gaagtcttaa cttgactcca tttactgcta
     4441 ctgggaattt cagagagatg atagataatg ttataatctt tattccattt ggcttgcttt
     4501 tgaatgtcaa ttttaaagaa atcggatttt tacctaagtt tgcttttgta ctggttttaa
     4561 gtcttacttt tgaaataatt caatttatct tcgctattgg agcgacagac ataacagatg
     4621 taattacaaa tactgttgga ggctttcttg gactgaaatt atatggttta agcaataagc
     4681 atatgaatca aaaaaaatta gacagagtta ttatttttgt aggtatactt ttgctcgtat
     4741 tattgctcgt ttaccgtacc catttaagaa taaattacgt gtaagatgtc taaatcaagc
     4801 aatctgatct ttcatacaca taaagatatt gaatgaattg gattagatgg aaaacgggat
     4861 gtggggaaac tcgcccgtag gtgtgaagtg aggggaaaac cggtgataaa gtaaaaagct
     4921 tacctaacac tatagtaaca aagaaagccc aattatcaat tttagtgctg aggaattggt
     4981 ctctttaata aatttcctta acgttgtaaa tccgcatttt cctgacggta cccccaaact
     5041 aagatttcat agatagattt tctgataact ttaaaaaaca cgaatagcgg aaaggatgtc
     5101 ttcattcgct aaagagtaat aaactacttt tccatcttga cgtgattttg ccattccatg
     5161 tttttttaaa aaacgtaaat ggtgtgacgt tgtagcgaca ctagcagtaa gtatttctgc
     5221 taaatcacag acgcacattt ccttataggt ttctaaagca tagaaaattt taattctaga
     5281 aaaatcgctg aaacatttcc ctaacacaag taagtttgaa aaatcttttt gatccaattt
     5341 attttttatt ccatttactt tttctttatg tacgacagtc acttggcaaa cttttttcat
     5401 tcatctcacc tcacattcaa acgattattt gaatgaagta tacctatttg atcactcttt
     5461 tgtcaatcta ccttaattcc aatacactat tataagcaat ttcatgtatt gaagtaaact
     5521 tacttgcaag tgcgtttttg attccaagtt aaaaacatat tattattcta cataatttcc
     5581 taatcaaata aaactccttt gaaaattgtt ctaaaggagt tttattattt tgtatttcta
     5641 ctaaaaatag cgtacaatgt aattactaag taattacgaa atgtaaaata atggaggaaa
     5701 taatatgatt acaaaaacaa gaaaacaagg aaattctatt atgttaacgg tccctaaaga
     5761 attcgatgtg cccaatggtg ttgaagtaga agctaagctt gtagagaatg gtatccttta
     5821 tgagtttgta gagcctaaaa aagaattttt tgattttagt gaagatattt tggctgatat
     5881 tctttcagaa ggttatagca aacaagagat cttaaaagaa tttagaaata gaaaaaatgc
     5941 gttaacttct gcatttaaat ctattgctga agatactgtt gcaaattcta aaccgatgac
     6001 aaaagaggag ttggcagcag aaattggctt atgagattct accttctaaa cacgtaataa
     6061 agtatctaaa aaaattaaaa gagaaaacat taaaagaaca atttttaacg ataatttatg
     6121 atgagatagc agttcgtcct cattcgggag aacaaaaaac gggtgattta tcaggaattt
     6181 gggctattgg attcaagtat gcaggtacga catatcgcgt tgcatatgaa attaaagata
     6241 atgcagtgat acctatctta ctatgtggaa cgcatgaaaa tttttatgaa caattaaaaa
     6301 agattaggta agaacccaat gtttcaactt gagaggatag acgacacttt aaaagataat
     6361 ttttaacagc tgagagttgt aataccctat aattttaaac cactaaccta tatatttatg
     6421 aaaaatagaa aaaattaaaa cgcattttaa gcctctttca aattaaaagc ttcatcctta
     6481 tgcacaaaga gggcttaaaa tgcgttattt tatattgttt tttagtaata ataaaaaaat
     6541 cactctaatt ctcccataaa tattatagat tcctaaagat taaaatagat tattccctat
     6601 tgtcttttaa gtagcggtag agtgttgcct tgctaatacc agtcatttcc gtaatttctt
     6661 ttacgctata aacctgactt ttatacaatt ttactgctcg atcaacatct ttctggttca
     6721 ctcgtggacg gccaccttta cgacctctcg cacgtgcact tttcaaacct tctttggtac
     6781 gttcaacaat taaatcacgt tcgaactgac taaatgcttg gaagacagtc atcattaagc
     6841 gtccgtgagg cgtgctggta tcgaagtttt ctttcaagct caccagctta acatggtgtt
     6901 cttcaaaata ggtaacaagg tcaatcaaat cctttgtact acgacccaat cgagaaaaac
     6961 tttctacaat taccgtatct cccggacgaa ttttatcttt taaacgattt agttggggac
     7021 gaaccgcttt ggttcccgtc attttctcag ttaggatttc attacaattt tgttcattga
     7081 gtaagtctaa ttgacgattt agttcttgtg aacgtgtact cactcgggca taaccataaa
     7141 tgtatccact catgcttttc ttcctttcgt actttaattc tggcttgatt gtatcataaa
     7201 agggagttat agatacatag ttaatgcaac aagttatgat acaaaatatg acctctttta
     7261 ttaaataaaa gaggtcattt cattttgtct cataatcgat tgtttttgat acgagtgtgt
     7321 atacgaaata aacaaaaaaa tcttaagtaa atttctagat acctaattta atcctaacta
     7381 gtgtatccta aagttaataa cgagtttatc acaatgaaaa ttataaaatg ctcccatatt
     7441 agatataaca tgcaatattt gaattagaac tacaaaattt attcgtttta taaagtaaat
     7501 aatgttgaga tactaaggaa tagaatatta taaggaattg agctgaatgt attggataaa
     7561 aaaagaataa ctttattaga gatggactat aaaaatgctc gtaattttct tctgaagtct
     7621 gaaagctatt tgaatgctca gctaccagaa tatattagtt ttgacgaaac tttaaaatct
     7681 gctaaaaaag ttttaacttc gccgtttgta taattaagcg agacaaataa aaaagccatt
     7741 tatgttacac tcaaattgta atacccgcta aagcatacaa ggagagtgaa cataaatgac
     7801 ttatacccat cttacaccaa acgagcttgt aatgatagaa gcatattttc atcaagaaac
     7861 tccggttgct atcgttgcga agcagcttaa acgtggacgc caaacaattt acaatgtcta
     7921 taactttctc aaatgtggtg gaacagcact tgaatacttt gaacaataca aagaaaataa
     7981 gcgacgttgt gggagaaccg aaatcatttt tcctgctgag gaaaaagaat acattgaaaa
     8041 aagatcaact gaaggttgga ccccagacgt cattattggc cgtgcagagc gaaccttctc
     8101 ttgttcaata agtaccctct atcgccggtt taagacggga gaattcaatg ttttacattt
     8161 accgatgcaa ggaaaacgaa aaccaaatgg ttataaggaa aaacgtggaa aacaggcatt
     8221 caaaagaaat atttctgaac gtaaaaaaga ttatgtcgtt ttcgaagaag aatttggaca
     8281 tttagagggt gatacgattg tcggcatcca ccataaaagt gccgtcatca cactcgttga
     8341 gcgactttca aaagccatta tcgtcttgaa accagaaggc cgtaaggcag ttgatattga
     8401 aaattcgatt aatgaatggc ttcaatccgt acccaaaaat cttttcaaat caattacctt
     8461 tgattgtgga aaagaattct ctaattggaa aagtattagt aatacgaatg atattgatat
     8521 ttatttcgca gacccaggaa cgccttccca acggggatta aatgaacatt caaacggact
     8581 cctccgaaaa gatggactac caaaagaaat ggacttcaac caagtcaatc aaggattcat
     8641 ctcatccgtt gcgtccaaaa gaaaccatat ccctagaaaa tcactaaatt accaaacacc
     8701 attagaagtt tttttgagtt acgtaaatgg aaagttttgt ctcgcttaat ttgacaaata
     8761 atacttcaaa aaaaaaccaa ccgaaagata taaaagatat tgtagataca cgaatgtata
     8821 aagaacgtga tgacttaaac tatacgctta ttatcaataa agatggaaac tattcatggc
     8881 gtcctctcgt tctactacat cctattctat atgtagattt ggttaattgt ctcactaaag
     8941 aagaccattg ggaggagtta aaaaagaggt ttagtgaatt taaaaaagat aaaaatatta
     9001 actgttattc tatacctgtt gaagcttcca aaggttcaaa aaggacagat cttggtgaaa
     9061 caatactaaa ctggtgggaa tattttgaac aaaaaagctt agctcagaat attaaatacc
     9121 aatattgcat gtttactgac atcgctgatt gttatccatc aatttatact cactctataa
     9181 catgggccat gtatggtaaa gaaaaagtta aaaatgatga aaataagtgt tcgggctcac
     9241 ttggcgataa aatagataaa aaaatatctg gaatgcaaca caatcaaaca aatggtatcc
     9301 ctcagggttc cgttcttatg gattttgtag cagagatcgt tctagggtat gttgatttga
     9361 gattatcgaa agaattaaaa aaattagaaa atttaaaaga ttacaaagta ctcagatata
     9421 gagatgatta tagaatattt tcaaataatc gtaatgattt ggaacaaatt atgaaagtgt
     9481 tatcagaagt cttatatgat ttaaatttta agttaaattc tagtaaaaca aaattatcag
     9541 atgatattat catagattct atcaaaaaag ataagctcta ttgggaagaa attaaggcaa
     9601 gtataagaat gaatgccaca aacttagatg gtgatagaga aattcgttat agtataaatt
     9661 tacaaaaaca tttattggaa ataaaaaagt tagcagataa gcatcctaat tcaggccaat
     9721 tgaaaaaagc actttctgag tttaataaag aacgcattat taaaatcact tcaaagccga
     9781 acgatctttt accacttatt ggtatcctgg tagatatcat gataaataac ccgggttctt
     9841 tgccgcagtg tgtcttaatt atagggcagt tattaaattt cgaaaatgag agtaaggcta
     9901 gagaaatagt tgaaatgatt ataaacaaat atgctttaaa gccaaatact gaatttatga
     9961 tgatatggtt tcaaagattg accaccattt ttggagaaat gtttgatagt gttttatcat
    10021 ccgaatttgt atctaaaatc aaagatcctg tctctaatca gatgttttct gtagactggc
    10081 taaatgaaga tgctaagaaa tctttttatg aagggaccat taaagacgaa gagaaatata
    10141 ttaaaatgag taagaaaata tatattactg aatttgatga ttttaatgat tatccttttt
    10201 aagtgaaaat ttagaatgaa tataaaaaac attacaatct aattttttta aaaaaatgtt
    10261 atatcggaag taatctcttg atttctatac tataagataa gaaaaccact atttaacaag
    10321 gttattcgaa ccttatttta agttacttat tatttaatgt atcgaaaaag gtgaaatagg
    10381 tttgttataa taagaattaa gtaagacatg aattgtctaa gcaaatgtag cttatccatt
    10441 tcattaaaat acatatggtg aaaaaacaag ttcaaatagt aactaatttt ttaaaaaact
    10501 gatttataaa aaatacaaca cgaatgagga aaataaccaa tgacagaaca aatcgaaaaa
    10561 aattcaaaaa gcttatatca ggcactgtgg aattccgcag atatcctgcg ttcaaaaatg
    10621 gacgctaatg aatacaagag ctatttacta ggcttggtct tttataaata cttatccgac
    10681 aacatgctgc ggtatgtctc cgttctattg gaggaagaaa cggacgacct aaataaagca
    10741 caagaccttt acgttgaagc atttaaagat gcagatataa aggatgatct cttgtatgaa
    10801 ctaaaagatg agttttcata taccatcgca cccgcattga cctttaccgc gcaagtggca
    10861 gccattcatg atggatcttt tcaattagaa gacctggtac aaggtttccg tgacattgaa
    10921 caatccagcg aaaactttga gaacttattt gaagacattg atctgtattc taaaaaactg
    10981 gggtcaaccc cacaaaaaca aaataaaacc atcgcagatg ttatgaaaga attagaaggg
    11041 ttaaacatgg cggggcatgc tggcgatgtc ttaggcgatg cctatgagta cttaattggc
    11101 caattcgcat ctgaatccgg taaaaaagcc ggtgaattct ataccccaca accggtcgca
    11161 aaattgatga cacaaattgt tttacaagga aaagaagata aaaaaggctt ttctgtttac
    11221 gacgcgacca tgggttcggg ctcgttactt ttgaacgcta aaaaatattc ccatcaaccg
    11281 ggaaccattt cttatttcgg acaagagttg aatacgtcta cctataattt ggcgcgtatg
    11341 aacatgattt tgcatggggt tcccattgcc aaccaacact tgcataatgc ggataccttg
    11401 gatcaagact ggccaacaga agagccaacg aacttcgatg gggtgctcat gaacccaccg
    11461 tattctgcaa aatggtcggc tgataaaggc ttcttagatg acccacgctt ctctgcctat
    11521 ggcgtattag cccctaaatc aaaagcggac tttgctttct tattacatgg ctactatcat
    11581 ttgaaagata cgggcgtcat ggccattgtt ttgccacatg gggtgctctt tagagggggc
    11641 gcagaaggga agatacgcaa agccttgtta gaaaatggtg ccattgacac cgtcattggc
    11701 ttacctgcta acatcttctt taatacgtcc attccaacga ccgtaattat cttgaagaaa
    11761 gaccgtgcga aaaaagatgt gctctttatt gatgcgtcac aagattttga aaaaataaaa
    11821 acgcaaaata cgctacgaga ttaccatatt gacgctattt tagaggccta taaaacccgt
    11881 acaattaaag acaagtatgc ttatgtcgca gaatttgatg aaattgtgga gaatgattac
    11941 aacttaaaca ttccccgtta cgtggatacc tttgaagaag aagaagtgat tccccttgac
    12001 tctgtttcga agtctattca agagactaaa gcagagttag cacaagcaga aactgagtta
    12061 tttaatatgt tgaaagaatt aaacgggaca actgaagagg cagagaatga gttacaagcg
    12121 tttatttcac aattgatgac tgatggtgat aataatgaat aaagaaaggg caatacctaa
    12181 agttcgtttt aaaagcttta gtggagactg gaaaaaatta aaattaagtt cggttactag
    12241 tagagtccga ggaaatgatg gaagaatgag cttaccaaca ttaacgatat ctgctagaaa
    12301 tggatggtta gatcaaagag aacgtttttc aggtaatatt gcaggaaagg agcaaaaaaa
    12361 ttatacgctt ctacgtaaag gtgaactatc ttataataaa gggaattcaa aactagctaa
    12421 atatggagtc gtatttatgt tagataattt tgaagaagcg cttgttccaa gggtctatca
    12481 tagttttaaa actacaaatg aagcatcatc aaaatatatc gaatatttat ttgaaactaa
    12541 aaagccaaat aaggaattaa gaaaacttat aacatctgga gctagaatgg atggattatt
    12601 aaatataaat tatgatgact ttatggggat taaaataact attcctaaaa ttaaagaaca
    12661 aaaaaaaatt ggatccttat tcaaacaaat agatggcact attacccttc aacagcaact
    12721 cctaaccgac tacaagcaat ttaaaaaagc gttgttgcaa cagctattcc cacaaaaagg
    12781 agaatcagta ccgaaaattc gatttactgg gtttagtgat gattgggaat tgaaagaatt
    12841 aaaagaattc attggtgaag atgtatctga tggtgattgg attcaaaagg aacacataca
    12901 tgaatcagga gagtatagaa tagttcaaac tggaaatata gggataggaa gatacataga
    12961 taagcctgag agtgcaaaat acctcaacca agaaagtttt gatgagttaa aagcaaatga
    13021 aataaaccct ggtgatattt taatatcccg attagctgat ccagcaggaa gagcacttat
    13081 tctgccattt acttcttcaa aaatggtgac tgcagtggac gttgctatta ttagaccaaa
    13141 taaaaatttc atttctcatt ttcttgtcac tagaatgaat tcatcagaaa cactaaatga
    13201 tatttcgaaa caagtaagtg gtacctcaca taaaagatta agtaggaaaa atttagaaaa
    13261 aatagaactt aatgtaccta atattgaaga acaagaaaaa atcggtcaac tcttcaaaaa
    13321 actggacgaa gccatcgcag ggcatgaaca gaaactcgcc acctatcaag aactcaagaa
    13381 agctctatta caacggatgt ttgtttaaag taaagagagg aggaacatca tggcgattac
    13441 ctttacatct gaatcggaat tagaacaaac cctcatccat cagttgattg ggggggaatc
    13501 acaatggacc tatcgcgggg atttgaatac cgaagacaag ctctgggata acctgcgtga
    13561 aaaattggaa cgcaataata aagatgtctt aaatgacgtg catttaaccg aacaagaatt
    13621 tcatcaagtg aaaaaccaat tacagtttgc caacttttat caagcgtcca aatggttggc
    13681 aggggagaat ggcattgcca aagtacaggt gcaacgggaa gatgcctcct tagggaccat
    13741 tcgtttaaaa gttttaaatc gggctgatat tgccggtggc atgtcgtcgt atgaagttat
    13801 taatcaattc caaagcttta aacaaagcac cgatgaccgg gaccgtcgtt ttgacgtcac
    13861 ccttttaatc aatggcttac ccctgattca tatcgaacta aaaaaccgtg ctcattccta
    13921 catggacgcc tttcggcaaa tcaagaagta cttggtacaa gggaaattta ccggtgtttt
    13981 ttccgctcta caaatgtttg tcgtctccaa tggcagtgat acccgttata ttgcggcggc
    14041 acaagatacc cggcttaatg aacaattctt agcaacctgg gtagatgcca acaatgatcc
    14101 ggtcaccagt tacttagcct ttgcgcaaca cgttttatcg attccgcaag cgcataaaat
    14161 ggtcacccag tatacggtca ttgacagtga taagaaagcc ttgctcttat tgcgtcccta
    14221 tcaaattcat gccattgagg ccattaaaca agcctctatc cggcaagaat cgggctatgt
    14281 ctggcatacg accgggtccg ggaagacctt gacttcttat aaagcagccc ggaacctctt
    14341 acatattccg tctttagata agacgatttt tattgtagac cgcgttgatt tggaccaaca
    14401 aacaacgtct tcctttgctt cttatgcaga gaacgatgtc attcaaattg acgaaacgga
    14461 taacgtcaaa gacttgatta aaaaactatt atctgctgat cgtacggtca ttgtgaccac
    14521 cattcaaaaa ctgaatcatc tgatgcgacg cattgaaggt aaagaaacca caccccgcta
    14581 taaaaaatta agagcattga atgttgcgtt tgtggttgat gaatgccatc gggcagtctc
    14641 ccctcaaaag aaacaagaaa tcgatcgttt cttcaggcaa cctttgtggt atggctttac
    14701 cggcaccccc atttttgccg aaaatgcaaa aaaagaaacg ggaaacttag ctcgtacgac
    14761 ggaagaacaa tacggcagtc gcttgcatga gtacaccgtt aaggaggcca ttcacgacaa
    14821 ggcggttctg ggcttccaaa tcgagtataa atccaccttt tcagacgatg aattagatga
    14881 cctcgtctta caagtagatc cagacgcaga tgtgcgtgcc ttaacggtct tagaaaaaga
    14941 agcgattatt cctaaatcgg tttatgaaca tgaacagcat atgttagaag tgattcattc
    15001 cattttgaat aaatcgcgcc ggaaattagg ctttcaaaat ggttctggga aaacctatac
    15061 agccctgtta accacgtcct caatcgcaca agcacaagcc tactatgact tattcaaaag
    15121 agttgtggca ggcgagacgg ctctggatat tcatgaaaag gtcaaaagtg tcttacctga
    15181 ttttccaaaa attgcgatga cgtactcgtt gtccgggaat gaggaggcat ctgtttccca
    15241 tcaagccaag atggcggaag cccttgcgga ttacaattcg ctctttgata cgaatttttc
    15301 cattgaaacc atgcgggctt acaatcgaaa cgtgaatgac cgcttagcgc gcaaacaaga
    15361 aaagtacctc tttcggaaag aacagttgga tttagtcatt gtcgtggacc gtctgttgac
    15421 gggatttgat gcgccgtgtt tatcgaccct ctttattgat cggcctgtga tgcagccgca
    15481 taacgtgatt caagcctttt cacgaaccaa tcgtttgttt gatcgctcga agcaatttgg
    15541 ccaagtggtg accttccaaa caccggcctt gtttgaagag aaagtgtcag aagcgctgtt
    15601 tctctactcc aatgggggtg agaatgctgt tttagcaccc acctttgaag aagcacacgt
    15661 cgcgtttaaa gaagcagtag cggccttacg ggacgtagca gtgatgccag atgcggttga
    15721 ttccttaaca gacgcgcaga aaaaacgctt tgccaaagca tttcaacggt tagataaaac
    15781 ctttgcggcg attcaggtct attcagagtt tgaagaagca gatttagaga gggattatca
    15841 gatctgtggt gaagaaatcg cgcactacca tggaaaatat gtcaatgtcc tggaagaaat
    15901 caaagaaaat atggattctg agcaaccaga gccgcttgtt attgatattg ccggttctgt
    15961 tgcaaagttt taaatctact atcaaataag gtagaataat agaaaaagat agtaggagga
    16021 atgacgatga atcattttaa aggaaagcaa tttcagcagg atgtgattat tgtagccgtg
    16081 gg
//
LOCUS       DAZABD010000061        15979 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000061 DAZABD010000000
VERSION     DAZABD010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.061"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            548..1351
                     /locus_tag="ACHALG_002132"
     CDS             548..1351
                     /locus_tag="ACHALG_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFN2587503.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKDLSDKLGNLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
     gene            1936..2391
                     /locus_tag="ACHALG_002133"
     CDS             1936..2391
                     /locus_tag="ACHALG_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587504.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            2510..3568
                     /locus_tag="ACHALG_002134"
     CDS             2510..3568
                     /locus_tag="ACHALG_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HFN2587505.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLDERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVSKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            3610..5559
                     /locus_tag="ACHALG_002135"
     CDS             3610..5559
                     /locus_tag="ACHALG_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFN2587506.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKSEDKENEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQL
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFK
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            5562..9203
                     /locus_tag="ACHALG_002136"
     CDS             5562..9203
                     /locus_tag="ACHALG_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3659 domain-containing protein"
                     /protein_id="HFN2587507.1"
                     /translation="MKFKKYLLQNLRIKRWVVRGLVIGGVLAVTLGAPFGMNDPTPDN
                     QDSKQETVINKNVAYAESKPAEQGMWTLYARILMGDKEVNDSAAKTGQMNLLGQGGIS
                     ADFPYSDISKQGNSIAGGNAGTQLASTLATYSYYGYIETVSGNAIASQASSILGSIGR
                     TIGSFIGSISLILSNIAESINELLGKFFMSMNIFSLMGWGEGGSDVSNPVSKALTSLL
                     NNLGLNNELFKAIASLSMLFFTAILGYQGMKLLSKDGVNSKGLSPVLEKWIVRIFVLF
                     IFLPVAGSLLGVYINGIQSAKKDGDIPTNVVSDYIINTRLWASTQNLAPSSDTGGDVP
                     DGKPTSEIKFVDTDYEPSKKSAMIRAINTSSYARQNNDLDSNSVGMALLKEWSSNDTF
                     NINTYAGDVNVAENDAYMGRAPAVWTGYNLGDGKEGSAIKKPTNLNGYIWSANQNVTD
                     DNRKPDGKGTAHNFDATQPWGVANNATFSTQSVALLLQSALDTSGAKFYAYNIAPSGI
                     QGSAKNMSTVKTEWRTASLVGHGAMGKFGSWLAMNAESIVLAVLYLACAWAMFNVNIV
                     KAFKREFIRIWEVVLTGSLASGAGVMAISLGVVTTSLIGLQLPIFLITFIRAFGDTAV
                     GFIPNTLVGDGVANIVRSAMILGVGYLLVFKKGEKVNNLINMLIGLPLEVAFGFDERA
                     KLLFGNRNDAVRVVDGVSKAIKKSQGAKNPYYDGGNSSVKDMVAGGLGAVGAQKMAER
                     LNNMGDSNADAKSIADKVIPETPEGMSFNGGGVGSTINNTSFKDLVRQDQLRNNSQQY
                     IDHLGRMVDENGNFVDENGEILKDSKYGKDAKGNFIDEKGRLVDEDGNLIDTNGKPLE
                     DSEDGNIGMDSEGRLIDEKGRLVDEDGNLVDENGRRLLNGDFGHDSNGRLVDEKGRLV
                     DEDGNLVDEFGNKLGKHSDGYNVNGDLVDEDGNIIETRDALVADRNRELEQQRHTPIA
                     VAIPENEEDEITLDQTHQELRNEMSGEDVSDRVHRELGDGASGESQSRELNSNFDNSA
                     IRNDTVLKDRESLTKETDKEQSDVKKKFIPTPNVNQTDKYETNIGSGMSKDNQSIREP
                     ELTNKNNRTNNSKSTSDNSGKSNQSTKKTSTAKSAVEYLSKTGKNVYTSADKALAKAP
                     KGSLAGITYEAKNEAIKIGKGTAKVAKATATGASKTGKVVKKSTMSVKDYIVKYGNQK
                     STENKSEDE"
     gene            9603..9941
                     /locus_tag="ACHALG_002137"
     CDS             9603..9941
                     /locus_tag="ACHALG_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587508.1"
                     /translation="MFDSIKNLVSGMTPDMKTLVQVVLYFLAFVFSLKPGFTAMKFFG
                     DAKWGDGLKYVGAIIAIFIIPVITTVMLYTMGTKTGNDLNQKANIISALIPVVGIYLV
                     SKFPKNELLG"
     gene            10027..10272
                     /locus_tag="ACHALG_002138"
     CDS             10027..10272
                     /locus_tag="ACHALG_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587509.1"
                     /translation="MREESKCKMVFDREKMLAHANEVLMSSLKGTELAKIMNMNVNQF
                     YDYRNGSKKIEKARLETLIKFEKAYVYMLDKQKRTID"
     gene            10292..12448
                     /locus_tag="ACHALG_002139"
     CDS             10292..12448
                     /locus_tag="ACHALG_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587510.1"
                     /translation="MAKFKDITESINYVAETVPVVALSLKPQLLEVYKKEEKEAKAKK
                     AKAKVWKEFDALLTKDLPKKNYMVIEDVQKLNSEGVIDEDDAILDSEKYVVLLKSSLN
                     YVADLPEKFKEKLLAGVRFMDSSGQLLVIPDDPNVFESEVEEATDFYSAVEQLEVATN
                     GLERIAETFNSSEEVEFEAVAENVPDEKEALFVDDNKPTEAENQEVSIEETQIQTETY
                     QQPKIDEKPESQSDKENYVYEAEKITSESDVPKNLDADFLEKNFEQIIADKFQPVVLD
                     EINDPVDLNEEMLSIPELKPLLFLAKESMNARISIANNKINSELNKTKEQLILKGKQM
                     LADELLRIEEATSLNGQNQFTNTLSVAKESYEAEASKIDDLVMEKSNIIRKDYDMAKQ
                     AYLDAELKKLEQRYDAEHLPEVKQQEDEYRKATEESLDQQYQEAIESIKNDALKQSQE
                     AKEVVVERIINSLKPEIEKSLTDVQEKTLEIVEKTKSENEAEFHDTKEKTTDVVVKYF
                     ANKEEVEKQVANNTKELETKLVEQQKTIEEYKDRMKEVKDLAEGYKLNSEKLAEQSEA
                     LREDLSRSRSDLAQMAEKTKSLAVATENQSSSKRDFTSKKMATIISVAAMACGTILGS
                     SYFISHNNQQAQAQTQTSISRVENSIGTNTNSASTNSSYEATPTTEFKVGKDVVISID
                     GKTTKAEVISIDGEKAVVKTPDGNQYKIQAHLSDNN"
     gene            12460..12693
                     /locus_tag="ACHALG_002140"
     CDS             12460..12693
                     /locus_tag="ACHALG_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFN2587511.1"
                     /translation="MREEKIVYPVIQNNQENMTSSQHLQEAQLVYQVNELPQTNETMS
                     KSISLIGLLIVAIVITVGVYRKLNVKGSISDGR"
     gene            12683..13549
                     /locus_tag="ACHALG_002141"
     CDS             12683..13549
                     /locus_tag="ACHALG_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587512.1"
                     /translation="MADNSNYGFKHNLHQKPSETIVRVEWLEKHVNSTYSVTAKRLIV
                     SAIVVAVVVLGSIVYLVSDTIKLKFIFVPTLLMIIGFLILISQYMFIDPSSGSLISGS
                     IRLFKSVVNSARVSMGKKKPFRGTEILKVKQDGTIVYRDGSVALMFNLDGATSLTAFP
                     EEVMAQESKSIQYQNTRDRSLAEIKITSSQPQNTERQTRNMKIQKRNARSNKAVFDII
                     DQQERFVSEYIDGVKTTVIQYLILKSQSKMVLDEGVERLENYTSRGLYYSVTQLSAKQ
                     TKEILSDIYALK"
     gene            13564..15534
                     /locus_tag="ACHALG_002142"
     CDS             13564..15534
                     /locus_tag="ACHALG_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587513.1"
                     /translation="MEIMGRSQLLNFKKKRKTKKEQKTRNSKVVNNTFVSDLTSLVGY
                     QFTPSYIRTGKRWGTIVKLVNTYGMNRNYGFGWMVKAIPEVRVEGVKAYLIEADKPVS
                     QKDQQEIMHENVGGSTNSYENNLEGKNSDAEISKTVMRIMDLNTASLLDSMANKIIDW
                     KLEILLVSDSPNKISEQLIKLQRLYDEKMYGLKLMSYGGDQRDLFDRLLSEPIGNVYE
                     YTSMSSDFAGNDHAIRKGLDDANGWAIGTMSTSYASGVAFMALDDSFTSKVLVASHKN
                     ATIKGYDEKISASSLWGQLIANNAMANHKRTFHIVLNKFDYFGDPTGRTFVAKPSLNS
                     VIEKVNLAEEGLNSLELFGNQKDIGDLYRRNLDKLTQIFYLLAGRTLDSATRVLLKKE
                     LGNFYISQKLWDINYEERSKLLVNSSDTMPVLGDFISYLTNAETKAKKSGTEKEVDRI
                     IVLKDTLRTALDSFSRMFNQRTTLKEIGKSDKLQFYYNLGDLRAETNVMEAQFLNTFD
                     FITSQAKKDDIVMIHGFDKLSIETVGLIKKRLTMLEEDGIRLAFLFDTIGSGEDNEYK
                     EDIETADIFNTDGKIYQSFESDFNYTIIGKMTSGELMKYEKKVAPARKGEDDQKLSVD
                     LRQYLTTGRSNKFQIRRPVDLTNNFVIGEFIL"
     gene            15701..15928
                     /locus_tag="ACHALG_002143"
     CDS             15701..15928
                     /locus_tag="ACHALG_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587514.1"
                     /translation="MKPNIKILDRIFLGRDTEVILIQHEEGFEVSIGIQKLQKPHYCN
                     QLYKNFTDEEKARVFFNEVKGMREQYEVVEA"
BASE COUNT         5921 a         2239 c         3221 g         4598 t
ORIGIN      
        1 ataaatataa ataaaagggg aagctgaagc ttcctccttt tccttttttt caattattca
       61 aagttacaca agcaaaataa actcctagat agtgttagtt ttttttaaga ttttttattt
      121 ttaagatttt gaaataagag aggagtaatc ctcctttatt aaatatctgt taatatatct
      181 gttaaatatc tgttaatata tctgttaggg gaggcttaat ctcttgggag agtaagtatt
      241 aaaggggtat aaaggtaaca cttttggaca gtaaaggtaa caaaagtgga cagtaagggt
      301 aacagttttg gacagcaaag gtaacaaaag tggacatttg aggtatcaca ctaggataag
      361 acgttacctt cggttttctt gatttggaag gcgaaaaaat agaaaattag attagtaaca
      421 aatctttgtt gactgttacc tttagttgtg atttaattta ggtaacaagc agaaaaaatt
      481 gtttccttta attagtacca gattaacatg attaaaggag cgcaaaaaga agattggaga
      541 gtaatcaatg tctcaagaag ttaccgaaaa gattattgat agtgatgtag atttagtatc
      601 agaacaaaca aatgcaattg tgcgttataa caatggtctg aatacaacag ctttgcgagg
      661 atttaagcct gttgaaatgg atgtgttttg ggctatttta tcaaaaatga aacgtcaagg
      721 aacagcagaa gtaacttttg atttcaacct ctttcgttat ttggtcaaat acgacagaag
      781 agaaaaggac agtttttatt ctgtgttaaa agatttgagt gacaaactag gtaatttaac
      841 ctaccgtttt gaagattcag acgtctatga acagctgtgg ctttttcaaa aattccggat
      901 agataaacag aaggaaacag ttacaattaa ggcaaatgag gaatttgaat ttatcttaaa
      961 ctcaattggc aaaaacttta ctcgttttga acttgataat tttacaaaac taagaagtag
     1021 ctacacaaaa gaattgtatc gtcatctcat gcaatacaga aataaagagt ggcgaaatgg
     1081 ttattgggta gtcaaagttg aagattttag aagagcatta agtatacctt caagttatag
     1141 aatgacaaat attgataaaa gaatactaga gcaggcgaaa gaagaatttt tagctcctga
     1201 tgaaaacgga atacaggtat ttgaaacttt tgattatgaa aaaatctatg caaagaaagg
     1261 aagaagagtt gatcggctca gattcacttt tagtgagcca gaatcaaact tgccaaccat
     1321 ttcaatgcat aattggttag aagaagattg agaagaagca ggttgtaatt ttaatacaac
     1381 ctgcttcttt aaaaaagtat gggtaacact tttggacaat aaatgcaaga agtttttttg
     1441 taagcaagtt tcagtctaaa taatatttga aagaatggct aatctataca aattttaaca
     1501 actatgggta acacttttgg acaagtcaag aaaaaaaaca atttttctta atttgttgat
     1561 cgactaaagg taacactttt ggacaacaca gaataaaaag tccggtgata tcagttttgg
     1621 taggaaacat cttaatatct ttgtgagatt gaaggtaaca tttttggaca agtcaactat
     1681 ttgttaaaag ttaatataga gtaattagat aagttttttc ttttcgtctg ataaatggat
     1741 aatattagtt attttattgc taattagtct ttcatgatga ctgaaatagc taaaataaat
     1801 atctcgttgg ataattgctg atactttaag ggatatctaa atttgctgat tgatttattt
     1861 atgttattat gatattataa taacataaat gtgttacgcc cgaacctcat tttaaaaaaa
     1921 tgaaggggtg agacaatgaa tgctattaat tggcatgaat ttgttaacta tatggagagc
     1981 ttactagatg atctaaaaag ttttagtatt gctgatgcag taaagcatgg gttaccagtt
     2041 agtacaacga tgaaattacg aggattgaaa attccagatg aaattattaa gctgagaact
     2101 aaaacaatta ttgaaaccgc aaaagtcgtt gacaatatga accatgaact aaaaggcaag
     2161 aaaacaactt acgagaaaga ttttgaacgt tttttaaaat ctgttattgg aaaatatcta
     2221 ggtaaaaaat ctttcagtga attgagagat gaagagattg tacctactcc aatggttagc
     2281 aaaatccgaa aaaacaaagc cgaggggaat tactatcttg gtacaatagc aaaaatcatt
     2341 gattatttgg aaaataaaga gcaaaagaaa ggaacgaaga aaggaagata atttttgtgt
     2401 taaatattaa tggggaagaa tataccgaaa tgattcaaaa tcccgaacct agaattaccg
     2461 aaaaaacgtc tgattgtttg ctcttttatg aaagggggca gacaaactaa tggaaaaaaa
     2521 cgtcaattca acatctacca aagatcaata cagtattatt ggagggttgc ttggtgataa
     2581 ggataagaga acacgtcacg gattacgcat tatgcttgca acttttctag gagtgctttt
     2641 tttattaaca ggcttgggta gctacattta tcaattagga aactataatt attatctcgt
     2701 gaacaataca acacctatag gctcttcatt ggagtttgct cgatcaggag cagagatcac
     2761 tatttcagat gtgtggacag atcaaaacag agatgtaaca gtagtaaaac tcggttactc
     2821 gcaaaacgct agaaagttgt tatctacgca aggaaaaaac tacaacttat atatggcaac
     2881 gaaagaaaaa gatcgtccta aaaagcttga aatgagctat gggatgttaa gtactgatgg
     2941 ggatgctttc ttattcctaa aaggtaaatt agatgaaaga gcttatcaag tttttattgc
     3001 aaaccaagta tcactaattt ctggtgatga tacagctgac gatgatacca aagtcaatgt
     3061 gaatgaagca gattcagtta caaaagcact ttctcaatat tctatgaatg atgttaataa
     3121 tgaaggagta atttcttttg ggaaaaaagc aaaacctgtt aatactcctg ataatatcaa
     3181 tttcagaata aatccatatt cagattcaac taatgtctat caaggaagct ttcttaccaa
     3241 agatggcgat attgattatt caaaagttgt agcagttaca agtaagaata atgttgtaag
     3301 taagttagaa gatcagttaa atgcttcaaa tactaacatc aagaaaattg aagctacaaa
     3361 agctgagtat ctggaacgtt tacaacaaaa tcctgacgat aaagattcgc aagaaagtat
     3421 tcgtcaacta gacagtcgaa tggagaccga aattaaagca atcaatgata cacaaaaatt
     3481 gattgatcaa tacaaaaatg ctgactttaa caaagattct tttggagaca tgaagacgac
     3541 atttaagtac ctaactctga ctgactagaa gtaaattatt cttattttag aggaagtgaa
     3601 cacaaacaga tggaaaatag taccgagttt tttaagaata ttcaaactga atttaatcat
     3661 gctctcacac caatgaaacc accgaaacat aagttagtgg gacgtgatga cgaactccat
     3721 gagttgtcac tgatcttgaa tagaccggaa acaccgcaag ctatcttgtt aggtgaagcc
     3781 ggaacaggta aaacagcact tgcaagtgaa tgggctgaaa gacagcgaca agatggaaag
     3841 aaagtacaag tatacaaatt atcaatcgga caattgcgtg ttgggggaga tgatgtcctc
     3901 caaacacgtt tagaaacatt aattccaaga ttgaaagaat atgagcgaat tttaaaatct
     3961 gaggacaaag aaaatgaatt tgtcctcttc attgacgagt ttcataaagt tgttagctct
     4021 tttggtagtg gcaataaaat tgggggtgat tcgctaaaag aaccgttagc aaatgaatca
     4081 cttcaattga ttaccgctac tacaagaaaa gaatatgaaa actatatcaa aagagatgat
     4141 gccttaggga gacggttaca accgttagaa ttgaatgaac taaaaagcga tgttgtcgtc
     4201 gatattttga gacaatggct aaatacatcc tctgttaaag atgatcctca ggcttctttt
     4261 aaattatcgc aaaggattcc tgatgaatta cttcaagaaa tcgtacaagc aaacaagctt
     4321 tatcgtgagg atttgtccga accggctaaa tcaattgccg taatggagga tttgattgca
     4381 aaagatcgag tagacggaaa tgcttataat cgagaaagtc tattgcaaat atttggtaag
     4441 tataatattc agctagattc tgattttgat atggaaaaac ttcaagagga attaaagtcc
     4501 ataaaagggc aaccgttagc agtttatact ataacaatgt tgtttaaacg tatgaagctt
     4561 ggatttgatc gttttaaagg tcgaaagcaa ccaaaagcaa ctgctctgtt tacaggatca
     4621 acaggggtcg gtaaaactgc aatggctaaa gccgtggcaa gaggacttta tggcgatgaa
     4681 aataaagttc tcttagttaa tatgccggac tacaaaacaa aagatgatgc aatgcgtttt
     4741 agacgactca taggtactca tgtaaggcat gatccttctg cgattattgt gtgtgatgaa
     4801 tatgagaaag ctggtgtaga aacactcgat gcctttttat caattttaga tgaaggtagg
     4861 ataagtttta ctgaaagagg atgggatgat cgagaagttc acgacactgt ttcattgtca
     4921 aatacaattt tatttgcgac aacaaatgca ggtgcggaga tgttcgattc gttaaacaga
     4981 tatagctcac tatctcgtgt tacaaacaag gaaaattcta atgaatatag cactgaacta
     5041 aaaagagaat accgaaaagt cgcatcaggg atcaaacaag ccttgattgg tgaaggtcta
     5101 aagcctgaag ttattaaccg atttggacga atagttccat tttgggcttt atccgaacaa
     5161 actcttctgg aaattgctta taatgaaatg ttgaagagtg ctgaattagt acgaaaacat
     5221 ttaaatatag atttacgtct tccacctagg aaagaaagag tatatggtca aaatttttat
     5281 gcagacgatc tctccatgca cttagttttt gacatggctt cacaagagga ttctaaatca
     5341 gggggtgcaa ggaatatctt gcatttagtt gaatctgagt ttgaagaagc gattactgaa
     5401 gcaataatta cttatccaaa cgcaaaaata ttcgatgtta agttgagtga agccggagcg
     5461 tttgaaggaa aaggtgattc taatgctgaa attattgcaa agccttccgt tgctgaaagt
     5521 aaagaaaaca agattgtatt ttcgattgga ggggcgtagc tatgaaattc aagaaatatt
     5581 tactacaaaa ccttagaata aaacgatggg tcgtaagagg tctagttatt ggaggagtat
     5641 tagcagttac tttaggcgct ccttttggaa tgaatgatcc aactcctgac aatcaagata
     5701 gcaaacaaga aacagttata aataagaacg ttgcatatgc ggaaagtaaa ccggcagaac
     5761 aaggaatgtg gacactatat gctcgtatcc taatgggaga taaagaagtt aatgatagtg
     5821 ccgctaaaac tggacaaatg aatttgttag gacaaggtgg tatcagtgcc gatttccctt
     5881 attctgacat ttctaaacaa ggtaattcga ttgcaggggg taatgcaggt actcaactag
     5941 catctacttt agcaacttat agttactacg gatatattga aacagtctca ggaaatgcta
     6001 ttgccagtca agcatcatcc attctaggaa gtattggtag gacgattggt agttttatcg
     6061 gatcaattag tttaatcctt tctaatattg ctgaatccat aaatgaactc cttggaaaat
     6121 ttttcatgag tatgaatata ttttctctta tggggtgggg agaaggtgga tcggatgtat
     6181 ctaatccggt atctaaagca ttaactagct tgcttaacaa tttgggatta aataatgaat
     6241 tatttaaagc gatagcaagc ttatctatgc tctttttcac agcaatacta ggttatcaag
     6301 gaatgaaatt attgtcgaaa gatggtgtta actcgaaggg attatcacca gtacttgaaa
     6361 aatggattgt tcgtattttt gtattgttta tattcttacc agtggcagga tcgctattgg
     6421 gagtttatat caacggcatc cagtcagcaa aaaaagatgg agatatacca acaaatgttg
     6481 tttctgacta catcattaac acaagacttt gggcttctac tcaaaatctt gctccatcat
     6541 ctgatactgg tggtgatgta cctgacggaa agcctacatc cgaaatcaag ttcgttgata
     6601 cagattatga accatctaaa aaatctgcaa tgataagagc gatcaatact agcagttatg
     6661 ccagacaaaa taatgatcta gattctaatt cagtcggtat ggctctatta aaagaatggt
     6721 catcgaatga tactttcaat attaatactt atgccggtga tgttaatgtc gccgaaaatg
     6781 atgcttatat gggaagagcg cctgctgttt ggacaggcta taatttaggg gatggaaaag
     6841 aaggaagtgc aattaagaaa ccaaccaatt taaatggata tatttggtca gcaaatcaaa
     6901 atgtgacaga cgacaatcgc aagcctgatg gaaaaggaac agcgcataac tttgatgcta
     6961 ctcaaccttg gggcgttgct aacaatgcaa cttttagcac acagtcggta gctttattgc
     7021 tccaatcggc acttgataca tcgggagcta agttttatgc gtataatatc gctccttcag
     7081 gaattcaagg cagtgctaag aatatgtcaa cagtaaaaac agaatggcgt acagcatcct
     7141 tagttggtca tggagctatg ggtaagtttg gctcgtggct agcgatgaac gctgaatcaa
     7201 tcgttttagc agttctttac ttagcatgtg cttgggcgat gttcaatgtg aatatcgtga
     7261 aagcatttaa acgtgaattt attcggattt gggaagtagt tctaacagga tcacttgcat
     7321 caggagcagg tgtgatggca atttcattag gtgttgttac aacatctctg atcggactac
     7381 aattacctat ctttctgatt acttttattc gtgcatttgg tgatactgct gtgggattca
     7441 ttcctaatac attagttggc gatggcgtag caaatattgt tcgatctgct atgattttag
     7501 gtgtaggata tcttttagtc tttaaaaaag gcgaaaaagt aaataatttg attaatatgc
     7561 ttattggctt accacttgaa gtagcgtttg ggtttgatga acgtgctaag ttattatttg
     7621 gtaatagaaa tgatgctgtg cgagttgtgg acggcgtttc taaagcaatc aagaaaagcc
     7681 aaggagctaa aaatccctat tatgatggtg gcaacagctc agtaaaagat atggttgcag
     7741 gaggtcttgg agcagttgga gcgcaaaaaa tggctgaacg tttgaacaat atgggagata
     7801 gcaatgctga tgctaagtcc attgctgata aagtaattcc tgaaacacca gaaggaatgt
     7861 cattcaacgg tggcggagtt ggttccacaa tcaataatac ctctttcaaa gatttagttc
     7921 gtcaagatca actacgtaat aactcccaac aatatattga tcatttagga cgtatggttg
     7981 atgaaaacgg aaactttgtc gatgaaaatg gtgaaatatt aaaagatagt aaatacggta
     8041 aagatgctaa aggcaacttt attgacgaaa aagggcgttt ggtcgatgaa gatggaaacc
     8101 ttatagatac taatggtaaa ccattggaag atagtgaaga tggtaatatt ggtatggata
     8161 gcgaaggacg tttaattgat gaaaaaggtc gcttagtcga tgaagatgga aatcttgttg
     8221 acgaaaatgg acgcaggttg ctaaatggtg attttggtca tgatagtaac ggacgtttag
     8281 ttgacgaaaa aggtcgttta gttgatgaag atggaaatct tgttgatgag tttggtaata
     8341 aacttggtaa acacagcgat ggatataatg tgaatggaga tttagtcgat gaggatggga
     8401 atatcattga gacgagagat gcgcttgtgg cagatcgaaa tagagaactc gaacaacaac
     8461 ggcatacgcc aattgctgtt gcaattcctg agaatgaaga agacgagatt actttagatc
     8521 aaactcatca agaactgagg aatgaaatga gtggtgagga cgtgtcagac agagtgcatc
     8581 gggaattagg agatggagca tctggtgaaa gtcagtcaag agagcttaac agcaactttg
     8641 ataatagtgc tattcggaat gacacagtac ttaaagacag agaatcgcta acaaaagaaa
     8701 cggataaaga acaatcagat gtgaagaaaa agttcattcc tactcctaat gttaatcaaa
     8761 cagataaata tgaaacaaat attggatcag gaatgtcgaa agacaatcaa tcaattagag
     8821 aacctgaact gacaaataaa aataatagaa caaataattc taaatctact tcagataatt
     8881 cagggaaatc taatcaatca accaagaaaa catcaacagc taaatcagcc gttgaatatc
     8941 tatcaaaaac tggtaaaaat gtttacacct cagcagataa agctttagca aaagctccaa
     9001 aaggtagtct agcagggata acctatgaag ctaaaaacga agctattaaa ataggtaaag
     9061 gaacggctaa ggttgccaag gctactgcta caggagcatc caaaactggt aaagtagtga
     9121 agaaatctac catgtcagtc aaggactata ttgtaaaata cggtaatcaa aaatcaactg
     9181 aaaataagtc tgaagacgag taaaaagaag gagcattgat ttgctccttc tttttacttt
     9241 gatttctaac tgaagatgtc aaggaaaatt tctatttatg caaaaaaatt attttaaatt
     9301 gtctataaat gaattaaata tttactgttc aaagttgtat aattaaaaag ggcttatgct
     9361 ggtaaagaac agaattatta tctaaacaaa aacttgataa accagcattt ttagccaata
     9421 aaaaaagtga aataatttaa tggaaaatct cttttaatca tcgaaaattt atgttattat
     9481 gataacataa agatgtaaat atcgagacgt ctttttctta gttagatcat tgacaagtga
     9541 acaaataaga tttgtcgatg atataattaa cttattaaaa atattaaaaa ggaggttttt
     9601 atatgttcga ttctattaaa aatttggtta gcggaatgac gcctgatatg aaaactttag
     9661 tacaagttgt tttgtatttc ttggcattcg ttttttcgtt gaaaccagga ttcactgcaa
     9721 tgaaattttt tggtgatgca aaatggggag atggattaaa atatgttggg gcgattattg
     9781 caatctttat tattcctgta attacaaccg ttatgcttta cacaatgggt actaaaacag
     9841 gtaatgattt gaaccaaaaa gctaatatca tatcagcact tattccagtt gttggtatct
     9901 atctagtttc aaaatttcca aaaaatgaac tgttaggtta aaagtgaatt gaacaaattt
     9961 ggataatatg ccgatttgtt caatttactt tttaccaaaa aactacgctc caagcgtagt
    10021 ttagaagtga gggaagaaag taaatgtaag atggtttttg atagagaaaa aatgttagct
    10081 cacgccaacg aagtattaat gagcagtctg aaaggtaccg aactggcaaa gataatgaat
    10141 atgaatgtta accagttcta tgattatcga aatgggagta aaaagattga aaaagccaga
    10201 cttgaaacgc ttattaaatt cgaaaaagca tatgtatata tgctagataa acagaaaaga
    10261 actattgact gaaaaaagga gttttacaat aatggcaaaa ttcaaagata ttaccgaatc
    10321 tattaattat gtggcagaaa cagttcctgt agttgcactc tctttgaagc cacaattact
    10381 cgaagtgtac aaaaaagaag aaaaagaagc gaaggcaaaa aaagctaaag caaaggtatg
    10441 gaaagaattc gatgcgttgc tgaccaaaga tttaccaaag aaaaattata tggtaattga
    10501 agatgtacaa aagctcaaca gtgagggagt tatagatgag gatgatgcta ttctcgattc
    10561 tgagaagtat gtcgttctat tgaagtcaag tttgaattat gtagctgatc taccagaaaa
    10621 atttaaagag aagctattag caggtgtccg ttttatggat agtagtggtc aattattagt
    10681 tattccggat gatcctaatg tgtttgaatc agaagttgaa gaagctacag acttctatag
    10741 cgcagtagag caattagaag tagctactaa tggattagaa cgaattgctg agaccttcaa
    10801 ctcttctgaa gaagttgaat ttgaagccgt agcagaaaat gtaccagatg aaaaagaagc
    10861 tctttttgtg gatgataaca aaccaaccga agcagaaaac caagaagtaa gcatagaaga
    10921 aacacaaata caaacagaaa cctaccaaca accaaaaatt gatgaaaaac ccgaatccca
    10981 aagtgacaaa gaaaattatg tttatgaagc tgaaaaaatc acatctgaaa gtgatgtacc
    11041 gaaaaactta gatgctgatt ttttagaaaa aaattttgaa cagatcattg ctgacaaatt
    11101 tcaacctgta gttttagatg agattaatga ccctgtcgat ttaaacgagg agatgctatc
    11161 catacctgag ttgaaaccat tgctgttttt agctaaagag agtatgaacg ctcgcatctc
    11221 tattgctaat aataaaatca acagtgaatt aaataagacg aaggagcaat tgatcctcaa
    11281 aggaaagcaa atgttagctg atgagctttt gcggatagag gaagcaacct cattaaatgg
    11341 tcaaaatcag tttacaaata cactatcggt tgctaaagag agctacgaag cagaagcatc
    11401 taaaattgat gatttagtga tggaaaaatc caatatcatt cgtaaagatt atgatatggc
    11461 gaaacaggct tatttggatg ctgaactaaa gaaactagag caacggtatg atgctgagca
    11521 tttaccagaa gttaaacaac aagaagatga atatagaaaa gcaactgaag aaagtcttga
    11581 tcaacagtac caagaagcaa ttgaatctat taaaaacgat gcattgaaac aaagccaaga
    11641 agcaaaagaa gttgtggtgg aacgaattat caattcattg aaaccagaaa ttgaaaaatc
    11701 attaacagac gtgcaagaaa aaactttaga gattgtcgaa aaaacaaagt cagaaaatga
    11761 agctgagttc catgatacaa aagagaaaac aacagatgtg gttgtcaaat attttgcgaa
    11821 caaagaagaa gttgaaaagc aggtagctaa caacacgaaa gaattagaaa caaaacttgt
    11881 agagcaacaa aaaactatcg aagaatataa agatcgaatg aaagaggtca aagatttagc
    11941 agagggttat aaattaaata gcgaaaagtt ggcagaacaa agtgaagctt taagagaaga
    12001 cttatctcgt tcccgaagtg atctagctca aatggcagaa aaaacgaaat cattagcagt
    12061 tgctacagaa aatcaatcaa gttcaaaacg tgattttaca agtaaaaaga tggctactat
    12121 catttctgtc gctgcaatgg cttgcggaac gatcttggga tcgtcctact ttattagtca
    12181 taataatcaa caagcacagg ctcaaactca aacatctatt tcgagagtgg aaaattcgat
    12241 tgggacaaat acgaactctg cttcaacaaa tagtagctat gaagcaacac caactacaga
    12301 gttcaaagtt gggaaagatg ttgtcatcag tattgatggg aaaacaacta aggctgaagt
    12361 gatttctatt gatggagaaa aagcagttgt gaaaactcca gacggaaatc agtacaaaat
    12421 tcaagctcac ttatctgata ataactaagg agggctatta tgcgagaaga aaaaatagtt
    12481 tatccagtaa ttcaaaacaa tcaagaaaac atgacctctt ctcagcactt acaggaagca
    12541 cagctcgtgt atcaggtaaa tgaactacca caaacgaacg agacaatgag caagtcaata
    12601 tctttaattg gcttgcttat tgttgcaata gtgataactg taggtgttta ccgtaagttg
    12661 aacgtgaaag gaagtatttc tgatggcaga taactcaaac tatggattta aacataactt
    12721 acatcaaaaa ccttctgaaa caattgtcag agttgaatgg cttgaaaagc atgtcaatag
    12781 cacctattct gttacagcaa aaagattaat tgtatcagct atagttgttg cagtcgttgt
    12841 tcttggatca attgtttact tagtttctga tacgattaaa ctgaaattta tttttgttcc
    12901 aacgttatta atgataattg gatttcttat tctaattagc cagtatatgt ttattgatcc
    12961 atctagtggt tcattgattt ctggttctat tagactgttc aaatcagtag ttaattcagc
    13021 aagagtatct atggggaaaa agaaaccatt tcgaggcaca gagattttaa aagtgaaaca
    13081 agatggaaca atcgtatatc gagatgggag tgtagcatta atgttcaatc tagatggggc
    13141 tacatcacta accgcttttc cagaagaagt tatggctcag gaatcgaaat caatacaata
    13201 tcaaaatacc agagatcgtt ccttagctga aatcaaaatc acttcatctc aacctcagaa
    13261 tacggaaaga caaactcgaa atatgaaaat acaaaaacga aatgcaagga gtaacaaagc
    13321 agtctttgac ataatagatc agcaagaacg atttgtatca gaatatattg atggtgtgaa
    13381 aacaacagta atccaatatc ttatcttgaa atctcaatct aagatggtat tggatgaagg
    13441 tgtggaacgt ttagaaaact atacgagtag aggactttat tatagtgtca ctcagttgtc
    13501 agctaaacaa acaaaagaaa tattatctga tatttatgca ctgaaataga ggtgagagat
    13561 taaatggaaa ttatgggtag atcacaacta ttaaatttca aaaaaaagag aaaaactaaa
    13621 aaagaacaaa aaactagaaa ttcaaaggta gtgaataata cctttgttag tgatttaaca
    13681 tctttagttg gttatcaatt tacaccatct tacattagga caggtaagcg gtggggaaca
    13741 attgtgaaat tagtgaatac ttatggaatg aatagaaatt atggatttgg ttggatggtt
    13801 aaagctattc ctgaagtgag agtagaaggg gtaaaagcct atctaattga agcagataag
    13861 ccagtgtcac aaaaagatca acaagaaata atgcacgaga atgttggggg atcaacgaat
    13921 agttatgaaa ataatttaga aggaaaaaat tcagatgctg aaattagcaa aactgttatg
    13981 cggattatgg atttgaatac agcatcttta ctagacagca tggcaaataa aattattgat
    14041 tggaagctag aaatcctatt agtatccgat tctcctaata aaatcagcga acagctaatt
    14101 aaactccaac ggttgtatga tgaaaaaatg tatggattaa agcttatgtc atatggagga
    14161 gatcaacgtg atctgtttga tagactttta agtgaaccaa tcgggaatgt ctatgagtat
    14221 acgtctatga gttcggattt tgcaggaaat gatcatgcaa ttcgcaaagg tttagatgat
    14281 gcgaatggat gggctatcgg cactatgtct actagctacg caagtggtgt agcttttatg
    14341 gcactggatg attcttttac aagtaaagtt ttagtggcat cacacaaaaa cgctacaatt
    14401 aaaggctatg atgaaaaaat ctcagcatca tcactttggg ggcaattgat tgctaataat
    14461 gcaatggcaa atcataaacg aacttttcat attgtcttaa ataaatttga ttactttggt
    14521 gatccaactg gtagaacatt tgttgccaaa ccttcgttaa acagtgtaat agaaaaggtt
    14581 aatttggctg aagaaggtct taactctttg gagctttttg gtaatcaaaa ggacattggt
    14641 gatttatata gacggaatct cgataaacta actcagattt tttatttgtt agcaggcaga
    14701 acattagatt ctgcaactag agttttattg aaaaaagaat taggtaactt ttatataagt
    14761 cagaaacttt gggatattaa ttatgaagaa cgttcaaaac ttttggtgaa cagtagtgat
    14821 acaatgccag ttcttgggga ttttattagc tatttgacta atgctgaaac aaaagccaaa
    14881 aaatctggta ctgaaaaaga agtcgatcgt attattgtat taaaagacac attgagaaca
    14941 gcattagaca gcttctctag gatgtttaat caaagaacga cactgaaaga aatcggtaaa
    15001 agtgacaaat tacagtttta ctataactta ggtgatctaa gagcagaaac caatgttatg
    15061 gaagctcaat ttttaaatac ctttgatttc atcacatcac aagcaaaaaa agatgatatt
    15121 gttatgattc acggcttcga taagttatct attgaaaccg ttgggctaat taaaaaaaga
    15181 ttaacaatgc ttgaagaaga tggaattcgt cttgcctttc tatttgatac aatcggttca
    15241 ggagaagaca atgaatacaa agaagatatt gaaacagctg atatattcaa tacagatgga
    15301 aaaatttacc aaagttttga aagtgatttt aattacacta tcattggaaa gatgaccagt
    15361 ggagagttaa tgaagtatga aaaaaaagtt gctcctgcta gaaaaggaga agatgatcaa
    15421 aaactttctg ttgatttgag acagtatttg acaacaggaa gaagtaataa atttcagatc
    15481 aggagaccag tggatctgac taataatttt gttattggag agtttattct ttaagtttta
    15541 tctttgattt tgagtgtata aggaaaggat aagaaaaaac atgtatattt gagactaaaa
    15601 acagtcttag agaacatggt acgttttgtg aaaagagaac tatagaaatt tcataaagtg
    15661 aaaccttatt gagtgaaata attatagtgg aggttaaata atgaaaccaa acataaaaat
    15721 tttagatcga atatttctag gtagagacac agaagtaatc ctaattcaac atgaagaagg
    15781 atttgaagtt agtataggta ttcagaaact tcaaaaacct cattattgta atcagttgta
    15841 taaaaacttt actgatgaag aaaaagcgag agtttttttt aatgaagtca aaggtatgag
    15901 agaacagtat gaggtggttg aagcatgaat aagttaccag aaaagaaccg tgtatttaga
    15961 ttgaaaggtg atctcagtc
//
LOCUS       DAZABD010000062        15349 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000062 DAZABD010000000
VERSION     DAZABD010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.062"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(129..587)
                     /locus_tag="ACHALG_002144"
     CDS             complement(129..587)
                     /locus_tag="ACHALG_002144"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFN2587515.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(758..1903)
                     /locus_tag="ACHALG_002145"
     CDS             complement(758..1903)
                     /locus_tag="ACHALG_002145"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFN2587516.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPHHELHGVAKQQMSGFSGMISFTLKNEETAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(1916..2833)
                     /locus_tag="ACHALG_002146"
     CDS             complement(1916..2833)
                     /locus_tag="ACHALG_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HFN2587517.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3261..3824
                     /gene="efp"
                     /locus_tag="ACHALG_002147"
     CDS             3261..3824
                     /gene="efp"
                     /locus_tag="ACHALG_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFN2587518.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            3925..4095
                     /locus_tag="ACHALG_002148"
     CDS             3925..4095
                     /locus_tag="ACHALG_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587519.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEREETALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFHH"
     gene            4310..5569
                     /gene="lysA"
                     /locus_tag="ACHALG_002149"
     CDS             4310..5569
                     /gene="lysA"
                     /locus_tag="ACHALG_002149"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFN2587520.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKERSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            5556..6356
                     /gene="dapF"
                     /locus_tag="ACHALG_002150"
     CDS             5556..6356
                     /gene="dapF"
                     /locus_tag="ACHALG_002150"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFN2587521.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDTDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            6359..7240
                     /gene="dapA"
                     /locus_tag="ACHALG_002151"
     CDS             6359..7240
                     /gene="dapA"
                     /locus_tag="ACHALG_002151"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFN2587522.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            7237..7953
                     /gene="dapB"
                     /locus_tag="ACHALG_002152"
     CDS             7237..7953
                     /gene="dapB"
                     /locus_tag="ACHALG_002152"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFN2587523.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            7959..8654
                     /gene="dapD"
                     /locus_tag="ACHALG_002153"
     CDS             7959..8654
                     /gene="dapD"
                     /locus_tag="ACHALG_002153"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HFN2587524.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAGTVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            8670..9671
                     /locus_tag="ACHALG_002154"
     CDS             8670..9671
                     /locus_tag="ACHALG_002154"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFN2587525.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            9668..11029
                     /locus_tag="ACHALG_002155"
     CDS             9668..11029
                     /locus_tag="ACHALG_002155"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFN2587526.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYSKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            10989..12140
                     /locus_tag="ACHALG_002156"
     CDS             10989..12140
                     /locus_tag="ACHALG_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFN2587527.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSGAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFHYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSRSKEERVYNDEFIQSI"
     gene            12115..13284
                     /locus_tag="ACHALG_002157"
     CDS             12115..13284
                     /locus_tag="ACHALG_002157"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFN2587528.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPKHVKQAGIAAIEEDFSHYTGMRGLEELREAACMFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            13460..14839
                     /locus_tag="ACHALG_002158"
     CDS             13460..14839
                     /locus_tag="ACHALG_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFN2587529.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSVSDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            15120..15349
                     /locus_tag="ACHALG_002159"
                     /pseudo
     CDS             15120..15349
                     /locus_tag="ACHALG_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         5042 a         2723 c         3275 g         4309 t
ORIGIN      
        1 aaaagagcgc taaactgaaa actagctatt tgttttcccg tttagaactc ttttcagaca
       61 ataaatattt aataaaatac gaagataaga tagctatatt gcttctcatc tttacgttct
      121 tttagaaact atgcttctac ttcagaccat tcatcgcgtt ttgctaaaaa ttcacgagcc
      181 aaagcttgtg caccttctag tgtatggctg gcagcccagc cacattgtgt ttcattgctt
      241 gcaggtactt cagtagcttc aagaacatct ttcattgttg cttcaagaac atctaaaatg
      301 tcttcgtagc tttcatgatt caatacagtc aagtaaaagc ctgtttggca tcccattgga
      361 gaccagtcta cgatgtaatc tgcgtgattg cggattagtt cggcagtcaa gtgctcaagt
      421 gaatgtagac ttggcatgtc catgtgttct ttatttggtt gtttaaagcg gacgtcgtat
      481 ttaatgatga catcgccatg cgcacccgtt tttcgatcag ctacacgtac ataaggtgct
      541 ttgacttttg tatggtcaag attaaaactt tcgacattca ttctcatttg taagactcct
      601 atctgtttgt tcattcgttt tgatgctttt cactaaagaa ttatttagtg aaatttacaa
      661 aaacgtcttt ctacttgatt ctaacactta tcgtttccaa ggtgtagcaa tctgaaatat
      721 tttttagatt tttctgctaa gtcgtttatt gggacttcta gttcttgatt ctgtcaaatg
      781 cctgagctaa atcgtttatg agatcctgac catactcaat tcctacagaa agacggatca
      841 agccgtcctt tatgccagcc tcttctcgtt tttcttttgg aatagacgca tgagtcatga
      901 cagaaggaat ttccactaaa ctttctacgc cacctaaact ttctgctaaa gtaaatagtt
      961 ggagtgattc aacaaatgga atagcagttt cttcattttt taaagtaaaa gaaatcattc
     1021 cgctaaagcc gctcatctgc tgcttagcaa ctccatggag ttcatgatgg ggtaatcctg
     1081 gatagtagac cttttctact gctggatgag aaaatagaaa atcagctacc acaaaagcat
     1141 ttttttgatg ttcctccata cgaacactta gtgtcttgat tccgcgctga agtaaccaac
     1201 tatcttgggg accaagtacg gctccgatag cattttggta aaatccgatc tgatcggcta
     1261 gatctttatg gttagaagtc actaaaccag ctactacatc gctgtgtccg cctagatatt
     1321 tggtaccgct atggacaacg atatcagaac cgagatctaa aggtctttga aaataaggag
     1381 tagcaaatgt attgtcagca ataactatca atccatgttg atgagccaga gtggcacttt
     1441 tttctaaatc agtaatcttt agaagcggat tgctaggtgt ttcaagataa agtgcttttg
     1501 tattgggttt gatggattgt tctattttat caagattact cgtatctatg atcgtgtact
     1561 cgagaccatt tttagtaaga actttgtcaa atagacggaa tgttcctcca tagacatcat
     1621 ctcctaataa gatatgatcg ccagcttgaa aaagagaaaa aacggcatga atcccagaaa
     1681 gaccagaaga aaaagcgaat cctcgaacac cgccttccaa atcagcaatc agttcttcca
     1741 atgcaaaacg agtaggatta ccagatctcg aatattcata ttgcttaggc tggccgacgc
     1801 catcttgccg ataagtggat gtttgataaa taggtacact gacagctcct gttgtgggat
     1861 cttcactgat gcctccgtgg atcaattttg tttgaatgtg catgtttctt tcctcctata
     1921 agtaaatgtt ttttgataaa tagcggtctg ctgcgtctgg aaaaatcgta acgacacgat
     1981 gaccaggtgg taaacgacga atttctctta atgctgctgc aaaagccgca ccactcgaac
     2041 ttccaactaa tagtccttgt tctcgtgcta actgcctagt atagttaaac ccttctacat
     2101 cagagattgt ctcgatctga ttgattgaaa ggggagaaag aaacggcgga atgaattcta
     2161 caccgatacc ctcaatttcg tgaggggctg gatcaccacc atttaacaca gagccctctg
     2221 gttctacacc gattattcga atgtcgggat atttttcttt caaaaaggta gaagttccag
     2281 caaaggttcc accactccca attcctgcta caaaactatg gatattttct ttgatttcat
     2341 gaaagatttc tggccctaac gtatgatagt aagcagcagg attatcttta ttttcaaatt
     2401 gaagaggcag atagctgttt gatatctcct ctgcaagttt tttacttttg ctgatggcac
     2461 cgatgattcc ttgctcacta ggagaatgga tgatctttgc tcccaatgct ttcatcaatt
     2521 gttgtttttc taagctaaac ttctctggaa caacaaaaac cgttcttaat ttgtatgtga
     2581 gtgcggccag agcgatccca attcctgtat ttccggcagt tggttcgatg atcgtcgttt
     2641 tttcattgat ttttccttgg gcaaatcctt ctttgatcaa ataactgccc aatctatctt
     2701 tgatacttcc tccaggattt aaatattcta gttttgcata aatagcagaa cctttaggca
     2761 tagaatagtg ctgattgtca aagcgataaa gcggtgtgga accaatagct tctgtaattg
     2821 tcgagataat catatgggcc tcctaaaaaa agataataaa aaaaggagct gactattaaa
     2881 tcagctcctt gaaagacata gagaaaaatt gagtagataa aaagataagt tacctggtac
     2941 tcattctatg aagggcaatt aatagacatg acaaatagta tgctgctttt atgcaccata
     3001 caacagcaac gattcaattg tttttggttg ctgactgtca tgtgtatccc tccaagtaaa
     3061 atttttatta acgatagcat gaaaaaaaat aaaagtcaaa ggattgaaag aaaaaaatat
     3121 ttcgatagca tcgtttaccc gtgtttattt ttctgacaaa taggcatgag accttaaaag
     3181 atagagaata ctctagtttt ctggccggtt gtctgataca ataaagttga ttatgataaa
     3241 gaaaagaagg agcaaaaata atgatctcag caagtgattt aagagccggt atgactttcg
     3301 tacaagacgg caaactgatt aaagttctgg atgcaagcca ccataaacct ggtaaaggaa
     3361 atacagttat gcgtatgaaa ctgaaagatg tccgttcagg tgcaacatac gacacaacgt
     3421 tcagaccaga agaaaaattt gaaaaagcac acatcgacac aaaacctgta caatatttgt
     3481 acacgatgga tgatacagct ttcttcatgg atttggaaac atatgagcaa tatgaaattc
     3541 ctgtagaaac agtagaagaa gaaatgaaat atatcctaga aaacatggaa gtaaaaatcc
     3601 aattcttcgg tagcgaagtc atcggcgttc aattaccaac aactgtcgta ttacgtgttg
     3661 tggaaacaca accttccatc aaaggtgcaa cagtcactgg ttcaggtaaa ccagcgacaa
     3721 tggaaactgg cttagtagtc aatgttcctg acttcatcga aacagatgaa ttgttggaaa
     3781 tcaatacagc agaaggttct tatgtaaaac gtgcgtcaaa ataattcgct aacggattga
     3841 taagaatttg aaaacagagc ttgacctctt gaaaggcaga tcatcggatc tgcctttcaa
     3901 gaggtttttt gtggaatcag caaattgaaa aataaaaaag tatttgattt tatttatatt
     3961 ttgaaagatg aatggcaaaa gctagagaga gaagaaactg cacttattta tgaagaaatt
     4021 gaaatgattc ataaaattga aaatatccga actattccga actatattga agcgaaaata
     4081 ttttttcatc actaaatagt gttgaattta tgtttttgtg tataaatagc ttctaatagt
     4141 tcgggtattt tgtttttata accattttta ttcttatttt taaaaaaata tttcgaaata
     4201 tactagacaa ttcagaaaat tttgtttaat attattccaa taaaattcaa atctttatca
     4261 aatgaaattc caataaaaac ggaaaaaaag tgaaaagagg aatgaaaaaa tgacagaatt
     4321 gataattggc ggagtcacag caagcacatt ggcagaaaag tataagacac ctctgtatgt
     4381 gtatgatgaa gaaaagatga gacaaacgat gctgtcattc aagcgaggct ttgtatcgaa
     4441 acgttttgat acaaaagtac tttacgcctc taaagcattc caaacgattg agatgctgca
     4501 tttagttcgt gaatatggat tcgggctaga tgttgtcagt ggtggggaaa tctataccgc
     4561 tttgcaagct gatttcccta gagatactat ctacttccat ggtaacaaca agacgccaga
     4621 ggaactgatc tatgcacttg aaaatgattt gttgcatatt gttgccgata atttgatgga
     4681 agttgaatta ttagcagaat taagccaacg ctatttaaaa aaaatctgta tcatgcttcg
     4741 gttgaatgtc ggagtagaag cacatactca tgaatatatc gtcactgcgc atattgattc
     4801 aaaattcggc atgagctatg aaagtaaaga gtgccaacaa gcccttcagt tgataaaaga
     4861 aagaagctat ctagagttag aaggatttca tgctcatatc gggtcacaaa tttttgatgt
     4921 tactgcttgg ttagctgaaa tcgacaaatt gacgaattac ttaaccgatt ttgaccaacc
     4981 tctttccttg aatcttggcg gagggtttgg gattcgttat acacaaaatg atcagccaat
     5041 cccagtcgaa gaggcactaa aaaaactgat tgaatatacg gaagaagctt tggaaaaacg
     5101 aaaactgacc attcgtcaat tactgattga accgggtaga agtattgttg gagaagctgg
     5161 tacaacacta tacacgattg gtttcatcaa aaagacacca cataaaaaat attactttgt
     5221 agatggcggg atgacagata atatccgtcc agctctgtat caggcagaat acgattgtga
     5281 tttagcagca aaactgtcgg aagaaaaaac agagaaagtg acgattgcag gaaaaatgtg
     5341 tgaatcaggc gatgtggtaa ttaaagaagc ttatttgccg aaagcagaat cgggtgatct
     5401 tttagctgtt tattccacgg gagcttatgg atactcgatg agcagcaact acaatcgagc
     5461 gacacgacca gcagttgttt ttgtcaataa tggccaatca agactgattg ttcgcagaca
     5521 agcattttct gatctactaa ggggggaagt ttcctatgat ccttcataaa tataatggct
     5581 gcggaaatga ttttatctta ttagattacg cagaagacac agattatagt caattagccg
     5641 cttctttatg cacaaaagaa aaatatgata cagacggatt gatcgccgta aaaatcgatc
     5701 cgttggaaat gatctattac aacaaagatg gcagccgcgc gccgatgtgc ggaaatggca
     5761 ttcgctgttt tgctcgatac gtaaatgatc agaacatgat gaatgaacag gaattcaacg
     5821 tagagacatt agcaggcaca agaaaaatta cggtgttgga tccctcacct ttttattgca
     5881 gcgtagatat gggagtgcca gatgattctc cggaaaaaat caaagctaat caaacttatc
     5941 caataaaaaa ccaggaaata gagatcaatg gtgaaaaagt aacgatcact agtttatttt
     6001 taggaacgat tcataccgtt gtttttgttg aaaatgcgcg tgaggaagtc tggagaaaaa
     6061 gaggtgaact gatctgtcat catcctttgt tcaaagaaaa aaccaatgtc aactttgtag
     6121 aagtagtcaa tgaacaagaa ttgatcgtcc gtacctatga aagaggagta ggttggacac
     6181 ttgcttgcgg tacaggttgc tgtgcctctt atgtggttgc taaggaaact gggaaaattt
     6241 cagcagatca ggtagttgtt cacttggaac aaggagactt gatgatctct ggtgaggatg
     6301 cgatacaaat ggctggccca gcagttcatg aatggacaag aatattggag gaatgaagat
     6361 gataaaaggt tcgatcgtgg cgctgatcac gccgatgaat gaagagggat cagtagatta
     6421 tgctggtttg gaaaaattga tccaattcca tttagatgaa caaacagacg gattattagt
     6481 tttaggtaca acaggagaat cttctacgct cacacaatct gaggaggagc agatccttca
     6541 actgacagta aagaaagtgg ctggccgtgt gccggtgatt gcaggagctg gaacgaataa
     6601 tacgaaagaa acgattgaaa aagcgaagca ttttgcttct ttgggggcag acgcactttt
     6661 ggtcatcacc ccttactaca acaaaacaag tgatgcagga ctagctgctc attttacagc
     6721 aattgcagaa gcctcaccga ttccgcttat tttgtataat gttccttcta gaacaggcat
     6781 gtcgattcca atccatgtat tagtaaattt agcagaacat cctaatatca ttggtttgaa
     6841 agaagcctca ggagatatgg cttatgtgat ggatgccgct cggttgattg gagaagaatt
     6901 cttcctttac agtggaaatg atgatttgat cctaccagta atgagtgtag gaggaagcgg
     6961 tgtaataagt gtatgggcga atatccaacc taaaatagtc catgaattag taaaagatac
     7021 gcaggacgga agatggcagc aagcaaaaga aaagcaatta aatgcacttg aactgatcca
     7081 tgccttgttc agtgagacca atcctattcc tgtcaaagca gccatgtcat tattagactt
     7141 accttccgga cctctacgcc tgcctttagt ttctctttca aaagagaaaa agaaacaatt
     7201 agctcagctt ttgttaaaag aaaggacggt tgaaaaatga aaatcggact gattggcagt
     7261 ggacagatgg gaaatagagt cgaagaagtt gcacaagaaa gaggagatac ggtccttctt
     7321 ttacggacgg cacctaaaga agcaactacg cttgctttta cggaactgcc agaactgctt
     7381 attgattttt ctcatccaga taatttagag aaaatccttt cgtatagctt gtcttatcaa
     7441 cttccgttag tcattggcac gacaggttat acacaatcac agttccaaga aataaaagaa
     7501 catgccaaac atgtacctgt gatgtatagt gctaattttt cgttcggcat catggtgatg
     7561 aatcaactaa tcaagcaagc agctgcaatg ttgcaaggct ggcagatcga actgattgaa
     7621 aaacatcata gtcgtaaaaa agatgcacca tctggtactg caaatatgct gcttcaaaca
     7681 atccaagaag tgcaaaagct gcagcccgta tacaactggc aagggaaaaa aagagaagaa
     7741 aaccaaattg gtgtacacag tattcgtgca ggttctttac ctggtgaaca tgatgtattg
     7801 tttgctgcta ctgatgaaat atttacgatc aaacatgaat ctttttctaa tcgaatcttt
     7861 gctgcaggcg cagtagaagc agcggactgg ttgaaagatc agtcaccagg ttattacaaa
     7921 ttagaagatt tattaatgga caaaggggtg taaaaaacgt ggaagctcaa gaaatcatcc
     7981 gctacattca aacggcatct aaaaagacgc ctgtgaaagt ctatctgaat gtaaccgagc
     8041 caatcgaatt taaagaaagt aaagtttttg gtgaaggaag cagcttcgtt gtttttggtg
     8101 actatgagac gattgctccg gttttagaag ctgaatcaga aaaaatcatt gaaatcgaaa
     8161 tcgaaactgc tgcaagatac agcgcagttc ctttgctaga tattaaaaaa attaatgctc
     8221 gtatcgaacc aggtgctatt atcagagatc aagtgtcaat cggcaacaat gccgtgatca
     8281 tgatgggagc gattatcaat attggtgcag taatcggtga aaacacgatg attgatatgg
     8341 gtgctgtctt aggtggacga gcgacagtgg gcaaaaactg ccatattggt gcaggagctg
     8401 tcttagcagg tgtgatcgaa ccagcatcgg ccaaaccggt tatcgtagaa gacggcgtac
     8461 tggtaggtgc gaatgcagtg attgttgaag gtgtccatat tggaaaagat gcagtcgtgg
     8521 cagcaggagc agtggtttta gaagatgtgg cagcaggaac tgttgtcggt ggaatacctg
     8581 cacgtgtatt aaaaataagt gatgatcaga caaaagaaaa tacagcattg attgctgcgt
     8641 tacgcgaatt atagaggaga tggagaaaaa tgcgcgaaat agatacctgt attgtggggg
     8701 caaccggatt agtcggccaa acgatgctca aagtgttaga agaatatgat tttccagtag
     8761 gacgattgaa attattagct tcaaaacgtt cagccggtaa agtttgtgta ttcaagaaaa
     8821 agaaacacac tgtacaggaa ttgacagaaa cttcatttga aggatatgat ttagcgctat
     8881 tttctgcagg tgcttctgta tccaaagagt ttgcgccaat cgcacgaagt cagggactat
     8941 ttgtcataga taattcctca gcatggcggg aagattcgga tattccatta atcgttccag
     9001 aggtcaatct ggaagactat acattgaatc ggctgattgc aaatccaaat tgttcaacca
     9061 ttcaagcggt acttccgctt aaagctttac aagaagcctt tggtatccag cgagtcaatt
     9121 attcgaccta tcaagcagtt tctggttccg ggcaaaaagg cattgaagat cttcatcgta
     9181 cgcaaaatga cgaggaacca gttttgtatc cttataatat tagccaaacg gtgattccgg
     9241 aaatcgatca gcctttggaa aatgggtata caaaagaaga acagaaaatg ataaatgaga
     9301 cccgtaagat tctgcatcag cctgctttgc cagtatcagc aacctgcgta cgtgtgccaa
     9361 tcgaaaatgg acatggagtc tctgtggcag ttcaactgca aaaagagttt acgacggaag
     9421 aaattagaga gtgtctcagc cagtttccag ggattcaatt agaagatgat ctagcagcac
     9481 ataaatatcc gacgagcatt cttgctagag gaacagatat ggtttatgtt gggaggatca
     9541 gaaaagacac ttctttagag aacggattgt tgttctatac gactgccgat aacatacgca
     9601 aaggagccgc tgcgaatgcc gtacagattg ctgcagcttt aatgcagaaa atagcggagg
     9661 tggcaagatg agtaatacgc tgaaagtagc gaaattcggc ggaacatcga tgtctcatga
     9721 agagcaattc aaaaaagtcc gtacaattgt tctttcagat cccaatcgcc aagtgatcgt
     9781 tgtttctgct ttaggcaaac gtacgtcaga tgatgacaaa gtaacagact tactttattt
     9841 gatttatgct taccttcagc atcatgttga ttggacaccc ctttggaaaa ggatttgtga
     9901 tcgtttcatt caagtaagag atgttcttgc cctgcagtta cctattgaag atgaactagc
     9961 acgcatacag aaacaactgg aagacgaaaa gataactgaa gattatttag taagtagagg
    10021 tgaatatctg actgctcaac tgatggctga gtatcttggt taccagttta ttgacgcaaa
    10081 agacctgatt ttctttgact atgatgggaa aatcgatcag gaaaaaacag cacaggaaat
    10141 cacaaaagca tttaaaagat attcgaaaat ggttgttcca gggttttacg gggcgaaccc
    10201 tgcaggaaaa gaaaaattat tgggccgtgg gggaagcgac attaccggcg cgatactggc
    10261 gaagatgttg caagcggaaa gttatgagaa ttggacggat gtttcaggaa tcatgatggc
    10321 tgatccgcga atcatcgatc atcctaaaat catcgaggaa atctctttta gagaactgag
    10381 agagatggct tatatggggg ctaacgttct tcatgaagca gctgttttcc cagttcaaga
    10441 ggctgatatc cccatccaaa taaaaaatac caatgatcct ttcgctcaag ggacaaaaat
    10501 atcgaatcac gaaatagaaa aggaaaatgg actgacagga atagctggac gcaaggattt
    10561 tctttccatc actttattta aacgacatat gtccgatgaa atcgggttta tctggaaagc
    10621 aatgagcatt tttgctaaac atgagatatc aatcgaacat attccttcag ggatcgacaa
    10681 tatcggagtg atcgtctcag cagaagcgat tgccgataaa ttatttctca tcacgaaaga
    10741 attaaaagaa acacttggcg tggaagaaat cgtcgtgatt gaagacttag ctttgatctc
    10801 tgtcgttggc ggaccacata aagaattgat cggtttatct ggaaaagtgt tatcgatatt
    10861 gaacaaatta gaaattcgca catcgatctt atcacaagga gcacaggagt tgaatctgat
    10921 catcggtgta ccgaataacc aatatgaaac agtagtgaag ggtatctatg aagggatggt
    10981 gaatacggat gctgaaagaa tatcgaatgg cactccatca aattcctgaa atcggatttc
    11041 atgaattcaa gacaaaaaaa tatttatatg aacaagtaaa aaacagcggg gccgttatcc
    11101 atgaaatcgg cgaaacagga ctactccttt attttgataa tcagcaagaa gaaacgattg
    11161 cttttcgaac agatatcgat gcactgccag tcaaagaaga aacgaatctc tcttttgctt
    11221 ctaagcatga agggtatatg catgcatgtg gtcatgacgg acatatggcg atgcttttag
    11281 ggttgactga ctttataact aaacacattg aggatcttat ttataatgta gtactgatct
    11341 atcagccatc agaagaaatt tccggtggcg cacaaagcgt tattcagtca ggattgttgg
    11401 ataaatatga agtacaagca atatttggct ttcatctatg gcctggacta gctaagggag
    11461 aagttttctc tcgaccagga cctttgatgg cacaaagcag tgaaacagac attatcatta
    11521 aaggccagtc tgcacatatt gcttctagtg aaaaaggaat cgatagtctg gaggcagctg
    11581 tacgattttt gaagaatgtt tatgattttg accggcaatt accggcagag tggaatcatc
    11641 taatgaaatt tggccaaatc caaggtggaa cgatccgaaa tgttttagct ggagaagtta
    11701 ttgtatctgg aagtatccgt tcttacagtt cagatatgca aaattatttg aagaggggat
    11761 tagaccattt agcagaacag tttcactcta cagctactat ttcttttcac tataatgacg
    11821 gctatccagc tgtaatcaac catagaggct tatatgcagc actgttagat tcaaacaaac
    11881 tacatgaatt agatgctcca gttcttcaag ccgaagattt tggcatatat acgcaacaat
    11941 atccatgtgt atttttcttt ttaggtgtcg gtgatacccc tgctttacat gatccgacgt
    12001 tcgattttga tatggatgtt ttagaaaatg gtgtagaatg gtacaaaacg attcttttta
    12061 cagaaaatct tcttgtaaaa atcgatagca gatcaaaaga ggagcgagta tataatgatg
    12121 aatttatcca atcgatttag tcccaggctt tctagaatag aagtatcaaa aatacgacaa
    12181 ttcgatcagc aaatatcgtc gataccagat gtgatcaaac tcaccctggg agaaccggat
    12241 tttccaactc ctaaacatgt caaacaagcc ggaatagcag cgattgaaga agacttttct
    12301 cactataccg gaatgagagg attagaggaa ctgcgggaag cggcttgtat gtttcagcaa
    12361 cagcgttatg gattaactta tgatccgcaa acggaagttt tgacgacagt tggtgcaacg
    12421 gaagcgattg cctctgcgtt gttatctgtt ttggaagaag gagacaaggt tcttattcca
    12481 gcgcctgctt attcaggata tcagccatta gtggaactag ctggtgctga attgatcccg
    12541 atagatactt ctgatacagg gtttgtctgt cagccggaac aatttgaacg agcatttgaa
    12601 caatatggat cagctgtcaa agcagtcatt ttaaactatc ctaacaatcc tactgggacg
    12661 acattgtctg caaaacaatt gaaagcaatc gctgaggttt taaaaaaata tccggtattt
    12721 gttataagtg atgaagtata tgctgaattg acttatagtg gaacgcatat gtccatagct
    12781 acttatcttc cagaacaaac aatcgtgata agcgggttat ccaagtcaca tgcgatgact
    12841 ggttggcgag tgggctttat ttttgctcaa aaagccctta ttgacgaact aattaaagtc
    12901 catcaatatc tggtgactgc cgctacaacg atgtcgcaaa aagctgcagc tgaagcgcta
    12961 ataaatgggg tgaaagatag cgagaatatg aaagagcaat atcaaacacg aagagactat
    13021 ctggtccagc aattggcgcc tttaggattt gaagtgaccc agccaaacgg tgcattttac
    13081 ttgttttgca aactcccagc tgccattcaa actgactctt ggcagttttg tgtagaatta
    13141 gcggaacagg caaaagttgc gtgtatccca ggaattgcat ttggcccaga aggagaaggt
    13201 tatatccgga tttcttatgc tagcagcatg gaaaaattac atgaggcctg caaacgaatc
    13261 aaagactttt taacagaaca gtgattcata gattttatca aaaaaacaat cgttaatttt
    13321 gcggtatatt ctgcaaaatt aacgattgtt ttttaaattt agttcaaatg aacgcgtttc
    13381 ttttgattca taactaatgt aatgttaagt ttaaattgtt tagaatgatt ttaaaagaat
    13441 attcaaggag gtttcatcaa tggaaaaaat caaagttgtc attgtaggtg catctcatgg
    13501 agggcatcaa tcaatcttgg aactattgac acgatacggt gaaaatgtag atatcacgct
    13561 atttgaagca ggagactata tttccttcat gtcatgtggt atggagctgt atttagaaga
    13621 caaggtaaca gatgtaaatg atgtacgtaa tttcagacca gaaaactttc cgcaatccaa
    13681 tgttcatatt ttaaataatc acgaagtaaa aacgatcgat gccgataaaa aaacagtaac
    13741 ggttactagc gtatcagata acgaaacaaa agaatatcct tatgacaaat tgattttgag
    13801 ttctggtgtc aaaccaaaca atctgccagt accaggagca gatctagaaa atgtctactt
    13861 gatgcgtgga tacaattggg caaccaaaat caaagaaaaa ttaaatgatc cggctattaa
    13921 aaaaatcgct gtcgtaggtg cgggttacat tgggatcgaa gcagcagaag caagccgaaa
    13981 agctgggaaa gaagtcgtat tgcttgatgt gatcgataga cctttaggca cttatttaga
    14041 tgctgagatg acagatattt tagaacaaca cctaaatgaa aacggtattg aagttttgac
    14101 aaatgcaaaa atcgaagctt ttactggtaa cggaaaagtt gaggcaatca aaacaagtga
    14161 aaaagaaatc ccagcagatc tagtcattca agctgccggt gtgaaggcaa atacagaatg
    14221 gttaaaaggg atcgtcgact tagatgaacg tggctggatc caaacaaatg aatacttgca
    14281 aacgaatctt ccagatgtgt atgcagtagg agatgctaca cttgcttatt ctatcccagc
    14341 acgcaaaaaa atgccgatcg ctttagcgac tgttgcacgt agagaagcac gttatgtagt
    14401 aaaacatctt tttgaagaag taccaaacaa accgttcggc ggagtcgtag gttcctctgc
    14461 attaagtgtc tttgactatc attttgcagc aagcggattg aatagcttta cagcagcaaa
    14521 agcaggagta gatgtacgag tgtcttatta tgaagatacg ttacgaccaa aatatgtacc
    14581 agaagaacaa ggaaatccaa aagtgtccat ccaattattc tcggatccaa tgacccatca
    14641 attgctagga ggagcagtat tatctaccta tgatgttaca ggtcaaggaa atgtattggc
    14701 actagcgatc caaaacaaac tgactttaga agaactagca gaagctgact tcttcttcca
    14761 gccaggcttt gaccgacaat ggagcatgtt gaatctagcc gctcaacaag cgttaggaga
    14821 agaaccattt gtagaataag aaaacgaaat atttgaaaag aggttgtgaa aaagctgcct
    14881 tgcatcaagt aataagaaca gccaagtaaa aacagcttct tatgttttta acttggctgg
    14941 acttattacc gcagatgcaa gcttttgaac accgtttatt ttcaaaaaag ggactgtgac
    15001 acgatttgtg taccgacccc caaaagttag accaaaaaat ctaacttttg gaggtcggta
    15061 ttttttatgg ttaaatatac ttttgaactt aaattaaaga tcgttcatga ttatttggat
    15121 ggcaaaggcg attggaccgt aaaaatccag atcaagagat tgaagcggaa atgcttaaaa
    15181 ttcgggagaa gcacaaggat tatggctgtt tacggatgac caatgaacta agaaaccgcg
    15241 gtttctctat caataaaaag aaagttcagc gcttgatcaa gaagctagga ataaaagtaa
    15301 cggcatacac gcgtaaatca cgtcgctaca attcttatcg aggaaaagt
//
LOCUS       DAZABD010000063        15127 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000063 DAZABD010000000
VERSION     DAZABD010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15127
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.063"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(87..266)
                     /gene="rpmF"
                     /locus_tag="ACHALG_002160"
     CDS             complement(87..266)
                     /gene="rpmF"
                     /locus_tag="ACHALG_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFN2587530.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(324..893)
                     /locus_tag="ACHALG_002161"
     CDS             complement(324..893)
                     /locus_tag="ACHALG_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFN2587531.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1079..1981
                     /locus_tag="ACHALG_002162"
     CDS             1079..1981
                     /locus_tag="ACHALG_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFN2587532.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2016..3800)
                     /gene="pyk"
                     /locus_tag="ACHALG_002163"
     CDS             complement(2016..3800)
                     /gene="pyk"
                     /locus_tag="ACHALG_002163"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFN2587533.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3874..4836)
                     /gene="pfkA"
                     /locus_tag="ACHALG_002164"
     CDS             complement(3874..4836)
                     /gene="pfkA"
                     /locus_tag="ACHALG_002164"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFN2587534.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5004..8318)
                     /gene="dnaE"
                     /locus_tag="ACHALG_002165"
     CDS             complement(5004..8318)
                     /gene="dnaE"
                     /locus_tag="ACHALG_002165"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFN2587535.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8549..8734
                     /locus_tag="ACHALG_002166"
     CDS             8549..8734
                     /locus_tag="ACHALG_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HFN2587536.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8796..9992)
                     /locus_tag="ACHALG_002167"
     CDS             complement(8796..9992)
                     /locus_tag="ACHALG_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFN2587537.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10162..11598)
                     /locus_tag="ACHALG_002168"
     CDS             complement(10162..11598)
                     /locus_tag="ACHALG_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFN2587538.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11891..12700
                     /locus_tag="ACHALG_002169"
     CDS             11891..12700
                     /locus_tag="ACHALG_002169"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFN2587539.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
     gene            complement(12886..15039)
                     /locus_tag="ACHALG_002170"
     CDS             complement(12886..15039)
                     /locus_tag="ACHALG_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFN2587540.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT         4425 a         3203 c         2674 g         4825 t
ORIGIN      
        1 atatttttta tttaccgact acaaaaaaat aaacctcaag gctccatggt tagccttgag
       61 gttttacatt ttctaattaa aatgtattaa gcttcttttg acattacgtc cttaccatca
      121 tagtaaccac aatttgcaca tacgtgatgg ctttttttca tttcaccaca gtttggacat
      181 tcgtttaacc ctttgattgt caatttgtag tgtgtacgac gttttgcttt tttagctttt
      241 gatgttcttc tagctggtac tgccattttg ctacacctcc ttgtaaagtc ttgtatctaa
      301 attagacaca atatattcca atcttacttg ttatcgtcgt cttttgattt agaatcaaaa
      361 agttcagata gttttgcaag acgaggatca attttattct cggcttcttc catttttctg
      421 cgctggtatt cttcttcgga aacaacttcc caatcattgc cttttggaag ttctgttgct
      481 gcttcctctt cttttgtcaa tacccgcatt ggaatcgcca ataagatgtt gtcttcaatc
      541 gactctgcta aattcaataa tggaccttca attagcaaaa tctcatctgc agacatttgt
      601 tcatctctac tttggaattg ttcttctgtc atgaatactt cgtctacaga aaaatccatg
      661 ggcagttcaa caggttctag tgaacgtgtt gaaggaactg tcaaagtcgt ctgcacagtg
      721 taatgaacga tatagccttt cttatcaact gagaccaaac cttcgacttc cactggcgca
      781 acatcaagta ttaaattgtc tcgcttcatg agagcggctt tcaagtcgag cgtctcatga
      841 aaagttaacg gtgtctcctg gtattttctc aattccaata aagaccattt catttttcat
      901 cactcctaag caacaaaagc aattatacag ggcaaaaagc atgttgtcaa tgaaattttc
      961 ttgacacctt gcagttcata aaaaaacaga aaaatcttta ttagaaagcc tctgtttgct
     1021 atcatcctca gaatcattta caataatcat tattccatta aaggagcgga tgatcttcat
     1081 gaacacgaaa gatatcgaat actatgtcca aatcgtcaaa caaaaaaatt ttacgaaagt
     1141 ggctaattat ttccgtgtga gccaacctac tattacatat gcattacaac gtcttgaaaa
     1201 agagtttgac accgtactta ttacaagaga tcgatcgcat cacgaattaa tcgtcacgcc
     1261 ttctggaaag cagctgtttc tccatgcaca aaccattctc caagagattt ctactgcaag
     1321 agctgaaatc actcagttgc aaaagaaaaa gcttcgtttt ggcatgcctc ctattatcgg
     1381 caatgaatac tttcctaaac tgtcaagtta ctttatcaag catgaattga tgaatcaaat
     1441 tgagattacc gatggaggct ctcgggactt atacgggatg atccgacaag ggaggatcga
     1501 tttagctgta ttaggctcaa cacagccaat cgtggatcaa tatttagaaa cagagttact
     1561 tttggacaaa cgtttcatga tcgtcgtgcc acctacacat cctctttcca agcaggatac
     1621 tgtctctttc aaagatatag cagatgaaca gtttgtacta ttgaacgaac attatgtcca
     1681 ccccgcagca ttcactaagc tttctaaaca aacacatact catccaaaag ttgtttatca
     1741 aagcaatgac ttgacgattt taaagagtat ggtccgagaa aatgtgggta tcggtttttt
     1801 ggcagagatt gctgtccatc ctgaagatca tttagtttgc ctccctcttt cagatgaagt
     1861 tcagcctcgc ttcttgattt cattggcaaa acgaacacag cagatcgtca cgcctcttca
     1921 agaacaagta gtccaagtta tccatgaatt cgttacagca caaaaaaaca gctcttcgta
     1981 aaagaagagc tgtttttatt ttttattaag ctaaattaga tagctgttgt ttcgccacgg
     2041 taaacgatgc cgcgacgtga atcaacggta atcaattcac cttctgtaat tgttgttgtt
     2101 gcgcctttag caccaacgat tacaggaata tcttgtgcaa ttcctacaac tgcagcgtga
     2161 gaagtcaatc cgccttcttc aacgataaca gcagctgctt tttcgattgc aggcatatat
     2221 tctttgtcag ttgatggtac aactagaacc attccgtctt ttgctttttt tacagcttct
     2281 tcagcagatg aagcaataac tgcatttgca acgacagatt tttgtccaac gccttgagct
     2341 tctaataatt tagaaccaat catttgaatt ttcatcacgt tagttgttcc gcgttcacca
     2401 actggtacac cagcagtgat caagattaaa tcgccttctg atgcgaaacc taattcaaca
     2461 gcttttttag ctgctagttc aaacatttca tcagttgttg atggtttttc agcaactgtt
     2521 gggtaaacgc cccagtttaa catcaaacca cgttttgtgc gttcgtcaaa agtaacagcc
     2581 aagatatctg catctggacg atattttgaa atcatttttg cagtgtatcc tgattcagtt
     2641 gcagcaacga ttgttttcac gcctagattt tttgcagcac gagctacaga taaaccgatt
     2701 gtttcagtta catctgtttt atcgaattcg ttcaattgga atgtacctaa ttctgataat
     2761 gcattttcag cttcttggtc gatacgtgcc attgttgaaa cagcttcaac tgggtagtcc
     2821 ccgtttgcag attcaccaga tagcattgtt gcatctgtgc catcaaatac agcgttagca
     2881 acgtcagaag cttccgcacg tgttggacgt gggttttctt gcattgattc taacatttgt
     2941 gtagcagtga taactgggat accagcagca ttacattttt tgataatgcg tttttgaacc
     3001 attggtacta gttcagctgg gatttcaaca cccatatctc cgcgggcaat cataacaccg
     3061 tcagaaactt tgatgatttc gtcaatgttg tcgatacctt cttgagattc gattttaggg
     3121 aagatttgaa catgagtcat gtctttttct tctaagattt cgcggatatc tagaacgtct
     3181 tgtgctttac gtacaaaact tgcagcgata aagtcgatat cgttgtctaa accaaaacga
     3241 atatcgtcag catcttttgg agtaatacct ggcaaactaa tagaaacgcc tggtgcattt
     3301 acccctttac gtgatcctag catacctgca tttttaactt ctacgactaa ttcacggttt
     3361 gcttcgtctt tttcaatgat ttgcatatcg atcaaaccat catcaaataa aatgtgtccg
     3421 ccaacgtgaa catcgtcaaa taatcctggg taagtaaccg cgattttttc ttttgaacct
     3481 acatgttcag gatccataga gatgcgtgtt tgatctccta cttcaaattt gatgtatcct
     3541 gcacgaccaa aatcagcttc tgttgtgcct tgaacagttg tacggatttc agctcctttt
     3601 gtatctaaaa gaataccgac atcttttcct gtctttttaa ctgcttcacg gaccatagtc
     3661 atacgtgata attgttcttc atggtcacca tgagaaaaat taaagcggaa gacatttgcc
     3721 ccagattcaa tcaattgaga aataatttct actgagttac ttgctggtcc taatgtacta
     3781 acgattttgg tttttttcat ttaaaaaact ctcccattca attttgttta tttaaagctg
     3841 tacacagctt aaatagcttg gtttctttca ctcttaaaat gaaagtgaat tatttagatc
     3901 atatagtgat aagtctggtt tatgtttgtt gttttcaagt gtatcaatga tgtcagacgc
     3961 aacgatttcg ttgtcatgga taccgacaca taatccacct tttccttctt gaagcaattc
     4021 gacagcgtaa gcaccgaatt tacttgccaa cacgcggtcg cgtgcgcttg gtgctccacc
     4081 acggacaacg tgtcccaaga tagatacacg tgtatgatag tcaccaaatt ctgaaagttt
     4141 ctcagcgaat tcatttccgc ccattacacc ttcagccaag atgatcaagc aatgtttctt
     4201 cccgcgatcg cggccttctt ggattttttt agcaactgaa gccatatcaa aatcatgttc
     4261 tgggatgatg atttcatccg ctccgccagc cacacctgac cataaagcga tgtctcctgc
     4321 attgcgtccc attacttcga taacgaatgt acgaacatga gaagtcgctg tgtcacgaat
     4381 acggtcaatt gattctagta ctgtattgat tgctgtatca aaaccgattg tgaaatcagt
     4441 cccaggaata tcattatcga ttgttccagg aatacctact gctgggaaac cgcgttttgt
     4501 caaagccatt gcgccatgat aagagccgtc tcctccgata accactagac cttcgattcc
     4561 gaattttttc aattgttcga ttccttttaa ttgaccttct tcagttgcaa attctggata
     4621 acgtgcagaa tacaagaatg taccaccacg ttggatcttg tcacctacat ctgccacatc
     4681 aagacgacga atatctccag caactaaacc ggcaaaacca taattgatcc cgtatacttc
     4741 cagtccttcg taaatacctt tacgaacaac ggcacgaaca gctgcattca ttccaggtgc
     4801 gtctccgcca ctggtcaaaa ttccgatgcg tttcataata aacattcacc tcattaaaat
     4861 actttatcct aagtatgact taggtttaca accatttttg cgttaaccac gactaacatt
     4921 ctaacattat ttttctcact tgtcttgatt tacggataaa aaaatgaaaa aaaatgaaag
     4981 caagtagtat tttttaccgt aagttatttg aaaaccacat tatcttcgcc aagcagataa
     5041 ataagttgcc cctttatact cttgctgtca tccacccaat aagcatctga cagaactact
     5101 tttcgttgcg ttttttcgtg atacataact actggtatat atcctgggtg tttctgaaaa
     5161 atcacttgta attgtttcat tatctcggga tcttcccgtt cttctgtaat acgcaaatag
     5221 caagtttgtg aaggatactg ggcttctaac tggttaggat cagcaaagct ttctccaatt
     5281 agttgcagtt cctgattata tttgctgatt tcgctccgcc cttgaacaaa gatcactcga
     5341 tcaacggcaa acaaattacg atatttacga taaagagttg gaaaaactgt gacagaaatt
     5401 tctccactac tgtcggttcc ttcaacaaag gccatcagtt ctcctttttt tgtccgaatt
     5461 tctctaactt ccttgatcat cactaataaa ttgactggtt gattagggac gatatctgta
     5521 atcagctgaa cttgtttagc taaacgaaga cgatcaaacc cttctgcagg atgacctgac
     5581 aaataaatac ctaagtactc ctcttccagc ttcaagcgtt cttctagtgt atagtcagcg
     5641 acttcttctt cttttaatgc catgattccc agcaagtcaa gacttccacc actgtaaacg
     5701 atattttgga tttttccttc gagatcaagc atcaattgtt tcctgttagg agataattca
     5761 tcaaaaactc ctactgctgt cagtgattgc agtaactcta atttcagcca acgcttatcg
     5821 atccgtatga ggaattgatc cactgactgg aacgggccat tttgttttct ttcgtttaat
     5881 atatcttcta caaaatcacg ccgaatacct tttatcgctg tcagtccgaa tctgatttct
     5941 tccttattga ccaactcaaa actggaatta ctttgattaa tggacggcga ggaaagtttg
     6001 agtttatttt tacgtgcttc tgcaatatat tctttcaatt ttgatgtgtt atgccgaaca
     6061 gaattcatca ggctggtaaa gaaagcacct ggatgatggg cttttagata cgccatttgg
     6121 aatcccacga atgaataagc aaatgcatgt gaccgattga atccatagtt tgcaaatttt
     6181 tcgatatatt catatacggt cgctgctgtt ttttccgaat atccctgttg taatgctcct
     6241 gatacaaaat gcgacttttc ttcatctaat acgtcttttt tctttttact cactgcccgt
     6301 cgcagaatat ctgactgccc taatgtaaat ccagccatag tcgaggcgat ctgcatgatt
     6361 tgttcctgat agacgatcac tccatatgtg tttttcaaaa tcgggatcag tgaatcatct
     6421 gggtagcgaa tggcttcttt tcctttcttc ctggcaatga aggtatctat attttgcata
     6481 gggcctggtc gatacaatgc attgactgcg gcaatatctt cgatgttttc tggatctaat
     6541 cttcgtaaaa cgttccggat accagcagat tcaaattgaa atactcctga ggtttcgcct
     6601 cgttgaaaca agagcagcgt ttttgcatca tttagcggga tctcttttaa tcggatctca
     6661 tacccttcta ctcgcttaac agctttcaaa gtaaaatcaa taatcgataa gttgcgtagt
     6721 cctaaaaagt ccattttcaa aagaccgatt gcttctacct cattcatcgt atactgggtc
     6781 agaaaagctt catcagaacc gcgttggagc ggaactagtt caagtagatt cttatcgctg
     6841 ataactacac ctgctgcgtg agtcgaaaca tgtcttggca atccttctaa aatcactgca
     6901 gttttgaata aaagttggtt tttttcgctt gcctgaacta attctactaa ttgatgcgaa
     6961 gttttataag cttgggtcaa tgtgattttc aattgattgg gtattgcttt tgaccagcgg
     7021 tttgcttcac tttgcgataa accaaacact ctagctgcat ctctaagaac catttttgct
     7081 gccattgtgc caaatgttgc gatttgtgcc atatgatatt gtccatactt ttctcgaaca
     7141 taattcaata cttcttcacg tcgattgtca ggaatatcca aatcgatatc tggcatcgta
     7201 aagcgttcgg ggtttaaaaa gcgttcgaac agcaaatcat attctaatgg atccacatct
     7261 gtgatttcta acacataggc tactaatgaa cccgcagcag aaccacgacc tgcaccggta
     7321 acgattttgt ttctatgagc aaaagccatt acatcccata cgatcaagaa ataatcatcg
     7381 aatcccattt gatggatgac agataactcg tagtccaaac gttcttggta ttcctttgta
     7441 tagtcagtga ttcggcgttc cacttgcgaa tagcataact ctcttaaata ttctccagct
     7501 tgttttccat taggaactgg ataatgcgga agtaatttct gatggaactg gaattcaaat
     7561 gaaatcgatt cagctaaacg ctgggcgcct attacagctt gggaaagacc cgctttttcg
     7621 aatcgtttga tcatttcttg ttctgaaaga agatagacat ttgctgttag tggtaaagat
     7681 gcagataaat tttctaaagg tatttggctt ccctctttga tatgtttcat cacttcaagt
     7741 gcaaaaagtt cttcttgttg taaactagcg atgggatgta atgcacaaag cgaataattt
     7801 tcttttatca catactgata ccattcaggc aattgatcag gcgtgttttc tgtcaagccg
     7861 atataaaaat ttctagtaaa aacattttca agtaaaaact gccaatgacc gaaaaagtta
     7921 tcctctttat tgaaagcctc agaaacagaa tgatttgccg gaacaacaaa cgctagatgg
     7981 tcggtttctg tcatttctgc aagttccaat ttttcttgga tcatttttcg gctggaaagt
     8041 ttcatcagct gctgataacc tatttcatct tttgcgtacg caagcaagcc ataatttttt
     8101 tcttcataag tataactaag atgtaatcca atgattggct gtactccttc ttctacacaa
     8161 atacgataga attcaggtgc accgtataag acatctttat ccgttatggc taatgaagtg
     8221 tagccttgtt tttttgcttc ttgtacatat tctcgaatac gtatcgtact ttgtaaaaga
     8281 gaataagaag taattgtata aagttgtggg aaaaacatct tgcattccgc cttttttttc
     8341 gttaaaattc actagcgtta aaaggtttga tccttttact ttcattaaga tagcaggcgc
     8401 tgaagttgat tattccactt catttgcttg cttgtaatct gtaggaatga gttctttcgc
     8461 aagaactcaa attttattga gatttggtct cgtttcttta cattcaaaga aaatctggta
     8521 aaattaagat agaaaagagg tgttctttat gcgatatatc gttacattat tctgggctgt
     8581 cgttctaggt caagtagtcg gttatattgg tgctgcatta acaagcggaa catatgattt
     8641 tactttgaca acaatcattt cattcattgc gggtgtcatt atccttttga ttggtgcagt
     8701 tgcgccaaga aaagaaacct ctgcccattc gtaaaaaaac agccaagagc ttgagatttc
     8761 tcaagctctt ttttattttc tactcctcac caccattata atctgctgcg aagaacttta
     8821 cgaaagttga ataaagaaag aagaatattt gacagaacaa acaaacttgt cacaataaaa
     8881 acagcactgt aattataagc atgcgcgact gtcgatccta gcatgggacc taaaacttgt
     8941 ccaaagttgc taaacatctg attgtagcta tagattcggc tgactccttc ttgaggcgta
     9001 atcttactga tcagtgtatt gacagaaggc atcaaagcgc cagttgaaaa gcctaaaata
     9061 aagcgaagta tacctaattg gagcggtgta gtgacgaatg ccataggtaa ataacagcaa
     9121 aatgataaaa tgagtcctcc aattagaact ttttgattcc caatcttatc ccctagtttt
     9181 cctagtttcg gtgaagaaaa gacagcggaa acaccagcta tcgaaacaat caagccgctg
     9241 acaaaaagga tattttctgt acttccgctc aattcacgaa tatataaggt caaaataggg
     9301 ctgatcgtag tgattcctat ctgtaaaatc aaggtcgtaa tgaagagacc gatcaaaata
     9361 gagagatgat ccatttttga aaaaatttct ttggtgctta tcagatcttt tttctcgatt
     9421 ggatggaaat cttctttgac taagaaaatg gtcaataccg ttgtgatcat caacaaagcc
     9481 cctgtgatca agaatacatt ttccatacca aaccactgag ccagtgcacc gcccattgaa
     9541 ggtccgatca gatttccagc aatcgctccg gtagacaatg ttcctaacgc ccatccgctc
     9601 ttttctctag gtgcctgtga agcaatcatc gctgtggcat ttggaatata tccagacaaa
     9661 atcccattcc aaaaccgcat gatcaaaagc caatacgcgt ttggtacaaa agccaaagat
     9721 cccattgtta atgtcatccc tgctgctgca cggatcatca tgagttttcg gccttttcga
     9781 tcagctagat ttccccagat tggtgcaacc acggcagatg caaaagcagt cacagagatt
     9841 gctagtcccg aaaacaattc gatttgatct tttggtgttc ctagttgctc aacataaact
     9901 ggtatgaatg gcataactaa actaatactt gcaccagtga aaaaacagcc aatccacgca
     9961 atcactaaat tttttctcca attaatctcc atctatcatc cctctttcgt ctcttttatc
    10021 atacctattt tcgaataaac aagcaacgat ctcgccagta aaaagagaaa aatcccgcaa
    10081 aggtcatttt atatgtaacg ttctttttat gtaaaacaaa aagagatcag aacaaatact
    10141 gttccaatcc cttctcttta gttaaattgt tgctagaatg atgaagttga taatgaataa
    10201 aatatcaacg atccacaaag caacagaaac ttgattgtat tttcctttga ttactttcac
    10261 aattgtatag aaaataaatc ctgctgcaat cccataggaa atgctatagc acaatcccat
    10321 aaagatcgat gcaaagaagg ccggtaaagc ttcttccata tctagccatt tgatgtctgc
    10381 aaaagaagcc atcatcatta ctccaactaa aatcaatgct ggcgcagttg cttgatttgg
    10441 tacaatggcg atcagtggag agaataaact acttaatgcg aatagcactg ccactactac
    10501 tgaagtcaaa ccagtacgtc caccggcccc gatcccagct gcagattcga cataagttgt
    10561 tgtattcgat gtaccaaaga tagcccccac agaagtagcg atcgcatcgg caaatagtgc
    10621 tttatccatt tttgttttga acccacgacc atcttccaac gcgatctcgt cttcttttga
    10681 gaaaatacct gtgcggcgtc ctgtaccgat aaatgttccg atcgtatcga aagtatcaga
    10741 taaactgaat gcgatgatcg tcatcaatac ttgaggaatc ttggaagaat cactgaataa
    10801 agattgcatg ccttcagaac caaatgctgc accgaaagtt gttcctaatt ctttaaatga
    10861 attacctaac gaattcgttt gccaatcaat ggaacttaaa tcaacaacac ccataaaaat
    10921 accaaggact gtggtcgcta cgataccgat aagaatagcc ccacggacat tttttacaac
    10981 taaaatggct gtcagtacca aaccaatcac agccaataag ataggagcgt tgtcaaagtt
    11041 ggctaacgca gggacgattc caccatttat cgtaacattt gtcgcttttc cgccttcaac
    11101 gacagagctc gaaatagctg actggtctgc tgaaaaagat aagaatcctg catttttgat
    11161 tccgacatac gcaacgaaaa taccgatccc gccaccaatc gcatgttgaa gactttcggg
    11221 aatcgcccga ataatcattt ttcgaatatt tgtgacagta ataaaaatat tgattaatcc
    11281 gcaaataaac accattgcca aggcttgttg ccaagaataa ccaagcccaa aaacgaccgt
    11341 aaaagtgaaa aatgcgttca atcccatacc aggtgcctga gcatatggta catttgcgaa
    11401 caatcccatt accagcgtac caataatcga tgcaatgatt gtcgctagaa atactgcttg
    11461 gaagggcata cctgatgctg acaaaatcgt cggatttacg aataagatat aactcatggc
    11521 aaagaatgtc gttactcccg ccattatttc agttgataca tttgtattgt ttttctttaa
    11581 accgaaaaac ttttccataa aaagttccac tccctgaatt tttttctgat ggaaaactat
    11641 aataataaaa aataagtaaa atagggcaaa tccatcgaac aatttcaaat tatatagacg
    11701 tttcattcgc ttttcaacgt ttttttaaca aaaaaagcga aaataatact taacgttcgt
    11761 gtttagctta tgtttagcta tttatatagc cataactgta gtttttgtta ggaatagctg
    11821 attgctcttg ttaattacct aaaatatgct agactaaaac aaatgctagt tgaggaagga
    11881 aggaccctta ttgaataaaa aattaattgc catcgatctg gacggaacta ccctcaatca
    11941 agattcgatg attacatcta aaacaaaaga gactctaaaa aaagcaatca atgccggtca
    12001 tcatgtcagt attgctacag gacgcccatt ccgaatgagc catcagtttt accaacaatt
    12061 agaattaaca acaccgatgg ttaattttaa cggcgcatta gtacacatcc ctaatcagca
    12121 ctgggatggt gaaagagaaa cgttgatcaa tcgtgaaatc gcttttgaaa tcttatcgca
    12181 aaaaaagcag ctaaatttag attttatcgc tgctgaaaat cgtgatactt tttttattga
    12241 cagttttgat ttctttgatg aaaaaatttt tgcttcttct cgtccaggag agaagaattt
    12301 gctttctcct aaaaatttga cgacaaatcc tacatctttg cttgtccgaa cagacaaacg
    12361 ttttgcagga gctgtatctg ctgaattaac acgtcaattt ggcagttatg tcgatgttcg
    12421 gacatggggt ggacctactg ccatcttaga gattgtttca aaaggaatcc aaaaagcaaa
    12481 aggcgtgcaa gagattgcta actacctgtc tatcgatcaa aaagacgtca ttgcctttgg
    12541 tgacgagcat aatgatttgg aattgttgga ttatgcaggt tggggagtag ctatggctaa
    12601 cggtactgac caactcaaag gcattgcaaa tgatgtcact cctttgagca atcaggaaga
    12661 cggtttagct gtttatctgg aaaaactact taaactataa tagagaatta gattccacaa
    12721 aaaagaggtt gtgacaattt ttgtcacaac cttttttgcc gagtaaacgg tgttcaaaag
    12781 ccagtttctc ggtatgaagt caaatttaag caaaaatgtg agaagctatt ttcacaaaat
    12841 ttttcttcat actcgaaact aagcggcttt ttcacgacct cttttttatt ttgccatgaa
    12901 catatcataa tacgcgtcca ttatttcttt agcaatcgtc aagtttatgt ggtcttgttc
    12961 gtctaataag ttcggcaaga caacactgat ggcaatttcc ggattgtctg ttggtccgta
    13021 tgccacgata ttactattga ctgtcgcaat atctggatga ccttccgcaa ccgtttctgc
    13081 agtccctgtt tttgcggaaa ggtccatttt cgcactggct aatggtttgg ctgttgtgta
    13141 aggatcggta ccatggacaa cttggtggaa tccttcacgg aggatcgcca tattttctgc
    13201 agaaatattg accgtatttt ctactgttgt gccaatttct ttcttcactt cacccaagtt
    13261 tccattttcg tcattgccat agatcgcttt gactaggtgc ggctgtactc gtttcccgcc
    13321 attagcgact gttgaagcat attgagctaa ttggattggt gtatatgtgt caaactgtcc
    13381 aaatgccaaa tcggtaaagt tacctggagc ataccattct ttgccattat ccgcattgaa
    13441 cttcttcata taatcaacag aacgtgagat cccaggagat tcattcggca aatcgatccc
    13501 agtcgttgta ccaagaccaa actctttgaa agagtctctc aattgctgat aagctttagc
    13561 ttcttcatct aaactaggta gtcccattcc aggtgtatag tctagtccta acatctttaa
    13621 cgccactttg atcatatacg tattagaaga caattccgat gctttgactg catcaagagc
    13681 aacttgtccg cttcgattaa agacagagct tttttctgaa gcaccttgca gacgaatcgg
    13741 ctcatcaatc aaaacttgat ttccgctgat cgcgttactt gcccagcctg ctgtcaaagt
    13801 accagccttg actacagacc ctggatcaaa ggcgctagta atcgtgccta aagcattctc
    13861 ggtgatttct ttggaacctt gttcatgact gaaaccagtc attgctagca cttctcctgt
    13921 ctgaggattc atagccactg cataagcacc aggcgaatac tgtgctttgc cgttgttgat
    13981 caatgtttga taattccttt tcaaaatttc ttctactttt gattggaatt ctgcattcat
    14041 agataacatt aaattagaac ctttttcccc agagaagatt tctttttgat tcgaaacatt
    14101 tccttcatta tcaagggaaa cttcatattg ggttttggtt ccttgcagga catcttcata
    14161 ttgtttttcc aaataacttt gtcctacacg gtcattccga gaataacctt ttgctaggta
    14221 tttctctgct tcatctgctg gaagtccttg cttttcagtc gtcacactgc caagaatact
    14281 tttcagtgaa cttgctgcat tatattctcg tgtccagtct gtgccagtcg aaactcctgg
    14341 aagttcagaa gcgcgttctg ccacaacagc taactcttga tcagtaacac cagagttttt
    14401 gatatagaca gtgtttaatt catatgcact attcatccgt ttaaagatgg tagcaatttt
    14461 taattgctcc tcgtttaaat taatctcatc agctgtgact ttattgacca tcgcactata
    14521 ttgttcgctg ttccctaatt gcttttcttt tgccgaaagt ctttttgtgg cttctttcaa
    14581 atgtttggga tcagccagcc aaaaatcttt tttatctcta tctgtcagat tttcatctac
    14641 cggaacatcg atcagcgagt tcagcttcgt tgcgatttca agcaaatctt tagccgtaac
    14701 ttgtgttcct ctcgtatatg tgatggcctg attcgcttta ttcgtaacta aagcttttcc
    14761 tgtggagtcg tagatcattc cgcgaggagt actttcttgg accgtaatcg tagaagtaga
    14821 cttcactcgc gcctccatct cttcgccgtt cacgatctgc aaataaccca atctagcaat
    14881 caacgcaaca aataacgtaa atataatgaa aaaaagaaaa tttaagcgaa aaggaacatg
    14941 ggaccggcga aacgttttgt tcttaggttc ttgtgcacct tttcttattt tttctaacca
    15001 agaagtattt aatatttttt tcataaaatc tttcatcatt tcctatcctt ccgcctattt
    15061 acattcatgc ttcattgtac ccaaaaactt cttaataaaa aagaaaaaat gggcggagca
    15121 agaaatt
//
LOCUS       DAZABD010000064        15063 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000064 DAZABD010000000
VERSION     DAZABD010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15063
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.064"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..319)
                     /locus_tag="ACHALG_002171"
     CDS             complement(26..319)
                     /locus_tag="ACHALG_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HFN2587541.1"
                     /translation="MELTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(347..1012)
                     /locus_tag="ACHALG_002172"
     CDS             complement(347..1012)
                     /locus_tag="ACHALG_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HFN2587542.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(1152..1784)
                     /locus_tag="ACHALG_002173"
     CDS             complement(1152..1784)
                     /locus_tag="ACHALG_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFN2587543.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     ALRPDVILMDLVMEEMDGIESTKAILKDCPKAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNDPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(1791..2858)
                     /gene="liaS"
                     /locus_tag="ACHALG_002174"
     CDS             complement(1791..2858)
                     /gene="liaS"
                     /locus_tag="ACHALG_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     LiaS"
                     /protein_id="HFN2587544.1"
                     /translation="MMGRISKAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLMAISLGVGLIVFLLLSLIQKAKYGKIEEKLRALSSGNYESKLLHQP
                     IPSASDDLYIKDIDMEIAKIKEKMIEVSSELQIMTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLTSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(2855..3586)
                     /gene="liaF"
                     /locus_tag="ACHALG_002175"
     CDS             complement(2855..3586)
                     /gene="liaF"
                     /locus_tag="ACHALG_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HFN2587545.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKYPRTKFQNFQLILGSLVIFFSLVNSPALWMMVVLAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMVQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAIISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKICSS
                     DYDENPRRLKIITNTLVGDVEVIRI"
     gene            complement(3754..4233)
                     /gene="greA"
                     /locus_tag="ACHALG_002176"
     CDS             complement(3754..4233)
                     /gene="greA"
                     /locus_tag="ACHALG_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFN2587546.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGD
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(4369..5544)
                     /gene="mltG"
                     /locus_tag="ACHALG_002177"
     CDS             complement(4369..5544)
                     /gene="mltG"
                     /locus_tag="ACHALG_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFN2587547.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPNGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELYQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEKAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVQKYVNNS"
     gene            5709..6869
                     /locus_tag="ACHALG_002178"
     CDS             5709..6869
                     /locus_tag="ACHALG_002178"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFN2587548.1"
                     /translation="MAKGKHQKKQQISLSFLAVLLLAVSFFSTGIITGWQLTKQPQPQ
                     IYKTEPAQLASNLTSNEQMLASKIDQLLQKSDYIGSIYVRKNNRVILEKGYGYANLRI
                     KESNSPSLYYQIGSIQKAMTALLILKQIQSGKITFDTKLSEFYPQIPGSQQISIKNML
                     YMRSGLHRTASPKKPMSDSEIIQFALHHLKFTGYDTYHYEPLNYTLLTGVLIKLMNQP
                     YETLLKKEIIRPLHLEHTDFYENVKNSPQHAVSYQMSATDDYSKALVEPETDIRNELG
                     TGNISMSVYDLNKFFTSVLSGDIIPKELLFSLWQNNGDKHPYSGGVYSGNDYILAQGN
                     INRFHAVAAMKKDTKDAIVMESNIQSDKNIKLPATDLRNQIYELMEGVNLKS"
     gene            complement(6909..7856)
                     /locus_tag="ACHALG_002179"
     CDS             complement(6909..7856)
                     /locus_tag="ACHALG_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFN2587549.1"
                     /translation="MDTILITGGAGFIGSTLANYLGKENKIVVVDDLSMGKKENLDME
                     KQVTFIEGDVSDPQLMERIMKQYQFAYIFHLAAVASVADSVERPLETHRVNFDSALLL
                     LELVRKYQSGLKRLVFSSSAAVYGDEPTLPKKEESVIRPLTPYAIDKFASEQYVLDYC
                     HLYDVPTSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQLAGEKTEFTLFGDGSQSR
                     DFVYIEDVIQALLLVAKEEKALGQQFNVGTGKSTTLLELIHSIDQILGTELALKYEAE
                     RSGDIRDSLADISKIRSLGYQPKFDILNGMERYLKTEID"
     gene            complement(7989..9380)
                     /locus_tag="ACHALG_002180"
     CDS             complement(7989..9380)
                     /locus_tag="ACHALG_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFN2587550.1"
                     /translation="MLNEKFLKYVKGWYFIYFGFILMLRAIGPYLLLPTRYDTLLFWP
                     AGIAGVVLIIIDAIIAFREKNLKKYDWLLIAFIGAMLVSSLMNLKYGYSENIKLIMWQ
                     LIFFFMIYQFGKDYSSNFFFKVFTWILSLVWFLANLVSLYWFVIQYGIKIPIKYKYYP
                     VRLGWLGNRLFGVYTDPNFGAVISLIVIIIAIYYLVTYKHLNLLFKIFFAINIFIQFS
                     FIALSGSRTTLVCLLIATFVFSFFTSYSSRFLKTNTLKRWLTALVVGLLSIIAMYYVT
                     DITAQAWAHVPHITEIKKDISDSSGTTSSNKVDLKRPDTDGGDVSNLRFGLWKSAIEI
                     FKSKPISGGSPGYYIEYAHDKLPYTLMGKDTLTAHNFIFLVMAATGGLGLLTLLTFLF
                     IQCFKTLRYCFTQPNLVKDKHFFAILMILTIAVSALFITELILVSTIGSFIFWGYLGS
                     IQKTISKEEGVTN"
     gene            9506..9916
                     /gene="tagD"
                     /locus_tag="ACHALG_002181"
     CDS             9506..9916
                     /gene="tagD"
                     /locus_tag="ACHALG_002181"
                     /EC_number="2.7.7.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0047348 - glycerol-3-phosphate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate cytidylyltransferase"
                     /protein_id="HFN2587551.1"
                     /translation="MKRVITYGTFDLLHYGHINLLKRAKQYGDYLIVALSTDEFNWNA
                     KQKKCYFSYEKRKQLLEAIRYVDLVIPEENWEQKVKDIKEYHADYFVMGDDWAGQFDE
                     LEQLTDAQVIYLPRTPEISTTQIKNELNSLNSDK"
     gene            10330..11130
                     /locus_tag="ACHALG_002182"
     CDS             10330..11130
                     /locus_tag="ACHALG_002182"
                     /inference="COORDINATES: protein motif:HMM:NF013753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFN2587552.1"
                     /translation="MRLRKQHYHYQSCGKYFTAHTYLVVPSCFISKQVHYTILEELTE
                     RQALKTLTKRCDLSVTTFQRTLSALSAMLRQMLDYLPHCLLFDEFRSLSISHGKFSFS
                     CMNRETGKLFDILSSRRKKDLIAYFMRFTRKAHMGVRYIVTNMNAPYFSLVKESFPNA
                     QIIIDCFHIGQHLNKFFDRVRKRVMKQLNQKDTSQAKYYRQLKPLYKLLLKSDDELDY
                     TTFKKWQNFQWRYLTESEVVDRLPSISYELKIAKQFIKSCLAIITRNS"
     gene            11239..11385
                     /locus_tag="ACHALG_002183"
     CDS             11239..11385
                     /locus_tag="ACHALG_002183"
                     /inference="COORDINATES: protein motif:HMM:NF013753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFN2587553.1"
                     /translation="MTKLENLRTHIKTLKRFAYGFRSFTNMRLWIFLINRLISYALKA
                     SNSY"
     gene            complement(11452..12879)
                     /locus_tag="ACHALG_002184"
     CDS             complement(11452..12879)
                     /locus_tag="ACHALG_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFN2587554.1"
                     /translation="MKKTVKDIFYNAIYQVFLIVLPLLTIPILSRRIGSTGLGIYGYV
                     FSISQFLMTVIAVGMNPFRIRNIAKSRKDKKALSLQFWNIYFIQFLIGLSVSFLYIAY
                     IYFFDVKYKNLYLIQLIFIVGLTLDISWFFQGIEEFSKVVIRNTVIKLSSVLLIIIFV
                     STKDDLWIYVFITSITNFLGSLVFWLSIHGKVGKPIFNSNVFKQLWKPAFIILTPQLF
                     MQVYTTLDKTIVGYFVDPTELSYYDQSQKIARIILAVLTSITIVMLPKISKAQAEGKS
                     ENILVYTKKSFDYTIIIALILFSVVFINTKEFVPWFFGENFKPMTINMLISCLIIVVS
                     PIGGVFANQFAIAIEKDKEYAYPMIIGAIVSIIGNVAFVPTYGAVAATIVLVVVELLV
                     FFFRVFFVRKEIKISYFFGKNVILFFIVNLTLTTWAYYFLPQLFSSSFLDMAIKSIMI
                     FIIYLPFVYFLFPDIRNEIRKITRK"
     gene            complement(12876..14018)
                     /locus_tag="ACHALG_002185"
     CDS             complement(12876..14018)
                     /locus_tag="ACHALG_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HFN2587555.1"
                     /translation="MKNIKKFFSRNIFPVFIKILPIQKNLIVFSSFGGRNYSGNPRVI
                     SEKIHEKKLNYRIIWLIKKEAIITDLPDYIKRIDSDSFLAAFYLYTAKYWIDDSRKTI
                     KYAKRKKQLYFQTWHGTPLKKIEFDAKDKLPKRYLSYAKKDSESITYLLSGNRYSSKK
                     YVSAFNIVPSKILEVGTPRNDELASSKEQVFDLKKKQINVLFAPTFRNNIEDNGITQL
                     EWLGVDNLREFFKKQQQELNFMTKFHPNVHSKLATDSKSIEYMKKHQITLINDGIPLE
                     MLFEEIDVLITDYSSIFFDFALTKKPIILFNYDEKEYSLERGFYIQLSELPIISVQDS
                     YGLVDVFKNQQEKLLKSSQELLDYIGNYETGASTEKVLELIERDIK"
     gene            complement(14022..15017)
                     /locus_tag="ACHALG_002186"
     CDS             complement(14022..15017)
                     /locus_tag="ACHALG_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFN2587556.1"
                     /translation="MHKISVIVPAYNVEDYLENCLESISNQTHSNIEIIVVNDGSPDR
                     SGEIAEEYKKKDMRLKVIHQVNGGLSAARNSGLDSATGDFVCFIDSDDWIEPDYLELL
                     YFGTQTYGADISVLKIKKISSLEQLKEHTIAERKWEIFDREQAMKNLFINNKIGYSAN
                     NKLFNRNLFDNIRFPLGKLMEDKATTYLLIDHSEKVAVNSSAKYHYYQSPKSILRGSF
                     NPRIFDSFEVHEDILIFIDKFYPELSINVRTRYVYEAIRMMMTMIKNNYTNKEDYLRS
                     INILQRFEKEVKIDPDFSPKLKKLVKFLLINKNLPYYMSSSKIASKFLIKIESVR"
BASE COUNT         4902 a         2864 c         2205 g         5092 t
ORIGIN      
        1 aaaacttgtg tcaccctctc tttttttatt gttttttaaa gtgataggtt ggctcctcca
       61 ggttgggatc ataatcaaca agtaaatcat atcctttaaa gaaccattca tctgcttcct
      121 cgataaaaaa aagtgtccca tccatttcca ctttctttac aggatgttct ggctgttcaa
      181 ctgacattcc aatcgaaaaa ccatcatgaa cctctgtttt gccgtagact tttccaaaaa
      241 aacgtattcc gtaagtttct ggcagatcaa tttctgagtc aaaccacttt tgtgcttttg
      301 gcgttaccgt taattccatc ccaacaacct actttctctt ttctttctat tgattcatct
      361 tatcgtctag aatatccaca tcgcctgtct ctccaatcac cagtagatta tcattttcgc
      421 gaacatattc atctgctgca ggagaagcga taaccgtttg atctactctt cgtatggcga
      481 ctactgttaa gtcaaaacgt tgcctgaaat ttaattcagc taatgttttg ttgaaaaatt
      541 taggattgga gactcggatt tctgctaaag aatatttatc tgacagctct aagtagtcta
      601 gaatattctt tgatactaag ttatgtgcaa tccgtatccc catgtctcgc tcagggtgta
      661 cgacacgatc tgctccgatc ttctctagta cacgggcatg gtactcattt tgggcttttg
      721 ctaaaatatt cggtacgccc atttccttta ccatcagagt aactaagata ctggcttgga
      781 tatcttctcc aatagcgaca attacatgat cgaaatttct tatacctaat gaacgaagtg
      841 tcatctcatc ctgtgcattt gccacaacag catgagtggc tatattcata tattcattga
      901 ctcgatcttc attactgtca atagccagta cttcttgtcc agcttcgatc agtgtacggc
      961 agatgcttcc gccaaatcgt ccaaggccta caattgcaaa attttgtttc accattttcc
     1021 tcctttagta tcttcttttc tcagtataac aaagcctttc caaaaacaaa agcaagcatc
     1081 attattgtaa aaaagccaag aaaaataata gttattttct cagcttttga ttatcctctt
     1141 tatcatcgtt tttatttcac caaaccatgt ttaaacgcat agatagctgc ttgagttcgg
     1201 tcttctacct ctaacttagc taatatattc gacacatgcg ttttaactgt tttcagtgtg
     1261 ataaacaatt catccgcgat ttcctgatta cttttccctt cagaaatcag catcaaaatt
     1321 tcattttctc gattcgttaa ttcttcatgc aatactggat catttcgccg actcattttt
     1381 tccatcattt ttgttgtcac ttcaggttcc aatactcgct cgccgcgttg cgtagccctt
     1441 atagcatcag ctatttcatg tgcggtagac gtcttcaaca aatatcctgc tgctcctgct
     1501 tcaattgcag gataaacttt ttcatcatca ataaaacttg tgacgataat gattttggct
     1561 tttggacaat cttttaagat tgcttttgtt gattcgatgc catccatttc ttccatgact
     1621 agatccatta aaatgacatc tggtcgaagt gccatcgcct tttcatagcc ttgacgtcca
     1681 ttttcagctt ctccaatcac ttcaatgtct tcttggatag ataaataaga agagacgcct
     1741 aagcgtacca tttcatggtc atctactaat aaaactttta tcatctttat ctatgcctcc
     1801 ttcatcaaag ggaccttgat ctccacgctc gtaccttgcc ctttaaaact aatgattttt
     1861 actgttccac caataccagc tactcgttct ttgatattgt tcaatccata acttcccgtt
     1921 ttggtttcat tcatattaaa tcctgtcccg tcgtcaatga tacgtaataa aagattgtta
     1981 tctattttat gcaaatagac ttctaattca ttagccttag catgtcttaa tgtgtttgat
     2041 aacaattctt gaacgattcg gaacaagtga tcctcaatgg aactagttaa ttttacatct
     2101 tctacatccc attttagcga aatctggatt ttattctgca attccttcaa taactgctca
     2161 atgccttgtt ttaaactttt ttcttctaaa ttgactggac gcagatgaag aagcagcgca
     2221 cgcatctcag actgggaagc gttgatgatc tctgccacca ttttcaactg cttacggaac
     2281 atttctggtg tctcactttt ttctgcttgc tccgtcaatg ccgacatcat catcatcgct
     2341 gcaaataatt gctgtgaaac tgaatcatga agctcacgag ctaatcgatg cctttctaat
     2401 tctaaaatct cttctttggt ttgtccatct acatattgag gacggctagt cataatctgt
     2461 aattcactgg atacttcgat cattttttct tttattttag caatttccat atcaatatct
     2521 ttgatgtaca gatcatccga cgcgcttggg atcggctgat gcaaaagttt ggattcataa
     2581 ttgccagaag aaagtgcacg aagcttttct tcgattttcc cgtattttgc cttttgaata
     2641 agtgataaca atagaaatac gatcaatcct acacctaatg aaattgccat caaatggaag
     2701 ataagcggaa cgtataataa acgcgcacgc agcaattctc cccaccaatg actttggtta
     2761 ctggcataaa aataagtgaa aagtgaaaat agaataataa ggaaagccgc aatccccgaa
     2821 taaacagcta gcattgcttt ggatattctt cccatcatat tcgtatcacc tcgacatctc
     2881 caaccaaggt attggtaatg atcttcaatc tgcgaggatt ttcatcataa tcactgctgc
     2941 agattttgat ctgttcgttt tttaaggaaa actgctcttc ttcaaacaag acatttccta
     3001 ctaatgtcgc atgctctaaa cgaatagcca ctcccagggg tactaagatc cgtgttctgc
     3061 ctatcccttt tcttacaatc acaatattat catcttttgg caaaagtgta ttacccaaat
     3121 cgatgatagt atcaccagaa atgatggcaa tgttgatatc atcccattca tagacgtcgt
     3181 ttccaatacg ttggttacca aataattgct gtcttttacg ctcattatta tgtgttttta
     3241 attgctctgt ttggaccatc atgatctgtt tttttcgcca aaaagcattt tttgtcaaat
     3301 ctattcctga aatctctaca cctttcagcc cgataaataa aacggccaat acaaccatca
     3361 tccataaagc aggactgttg actaagctaa aaaaaatgac caagctccct aagatcaatt
     3421 gaaaattttg aaatttagta cgaggatatt tgcgcatagc tagatatata ttgaaaatac
     3481 cgaatattac taaaagcaaa agtccagtat tgtttacgat ttgccaaaca gcaaataaca
     3541 gcagcagtgc ttcgactacc acaaaaaaac gccaagaact attcatcgct ttttctccct
     3601 cctgacacat tctcatttca tcatacctat ttttttgact gttggctata ggactttaga
     3661 tgtaagatcg ttcagtgaaa cctgcaatac tcaaagagaa aagatctaga gtgcgtcatc
     3721 agtaagatga actctctaga tctttcagtc agactacgct tgttctactt tgacgatttt
     3781 aactaacata tcgccacctg gtgtagcaat ggttacttca tcatttttac gtttgccaat
     3841 caatgcttgc gcgataggag aatcattgga aatctttcca gaaaacgggt ctgcttcagc
     3901 acttccgaca atcgtgtact cttcttcatc tccatcaggc aactcgatga acgtaactgt
     3961 tttaccgatc gatacctcat ctgcatctac gccgtcatta tcgatgattt gtgcaaaacg
     4021 gatcatattt tcaattgttg tgattcttcc ttcaacaaat gcttgttcat ctttagctga
     4081 ttcgtattcg gagttttcgg aaagatctcc gaaaccgcga gcgattttga ttcgttcaat
     4141 gatttctccg cgtttaactg tttttagttc ttctaattct ttttctaatt tttctttacc
     4201 ttctagagtc attggataaa ctttctctac catgtgtaac gtctcctttt ttcgataaaa
     4261 taccgcaaaa ggtaatcttt tgaggaatat agtccaaaaa agattaccac gcttttctta
     4321 aaatgtaaat gtttttctaa aaatgatcaa tgactagccc ctgtttttct aagaattatt
     4381 tacatatttt tgcactagtt ccatatgttc ttcatacgtt ttagcgaaat aaacatttcc
     4441 agtgctcgta tctgctacga aataataata gtcattttct gctggttcta atactgcctt
     4501 gattgccttt tcgcttggat tgtcaaaagg accaggccca tagccggtat tggtatacaa
     4561 attgtatggt gaatctacag ctgtatcttc atacgtcacc aattctttat gttctcctaa
     4621 tgcatataaa acagagatat ctgactgaag tggcatattt gccttgattc ggttaaagaa
     4681 gacttgtgca atatttttac gatcattttc ttttacgcct tctttttcaa ctagagaagc
     4741 caaagttaaa acttcttgta cagtcaaatt tttctgtttg atagctgaat aatagttttg
     4801 catgaccgta tttgtcttgt tcaccatctc aatcacaaga tccttaagcg aagtactcgt
     4861 gtagtaatca taagtagctg ggaataagta gccttccaaa cggtattttg tgttctcagc
     4921 tttagaggca ctttccaaca attctgggaa tttttgatac aactcgttaa agaaagtctc
     4981 atcatttact aaatctaaaa attctttctt gtctttacct gttaccttcg ctacccgctc
     5041 agcaatctga tcgatgtcgt agccttctgg aatggcaatt ttcccatctg caacttttgt
     5101 tggctcagat gtgccgcctt cttgcaactg cttgccaatc tcatcaagag tcatattagg
     5161 cgctaactga taataaccag cttgaaagcc agttaagttt ttaaattttg tataataatt
     5221 gaaaacaatc ccacttttaa taatattgtc tttctccaaa atttcgccaa tctgcttatt
     5281 agaagagccg tttgggattt ccacttggac taattggtcg tccgatttat ctaaaggctt
     5341 caacccgcta tctacataac gatatacggt aaacccgaga aatccgccaa taatcaataa
     5401 tgtaagagcg atcactaaaa tgatcttcct cacgatctta tcttcttttt tacgcagatt
     5461 tctccgcttg tcgattatat ctgctgtagc tgccttactg gcttcttttt tgtctttgct
     5521 tgggtgttgt ttgtttcctg acaaaacaac tcctccttac tatcatcttc gtaactctgt
     5581 ttattataca tgaaatttct actgccgtga attattttac caaatattat gtcaaaattg
     5641 taagtggcaa atgctctttt tgaggattac aatgaaaaat agttaaaaat ggatagaaag
     5701 gatttaatat ggcaaaagga aaacatcaaa aaaaacagca gatctctctt tcttttctcg
     5761 cagttttgtt gctggctgtc agcttctttt caacaggcat cattacagga tggcaattaa
     5821 caaaacaacc tcaaccacaa atttataaaa cggaacccgc acaacttgct tccaatttaa
     5881 catctaatga gcagatgctt gccagcaaaa ttgatcaatt actgcaaaaa tcagattata
     5941 tcggttcgat ttatgtacgc aaaaataatc gagtgatatt agaaaaagga tatgggtacg
     6001 caaatctacg cataaaagaa tcgaattctc cttctcttta ctatcaaatc ggttcgatcc
     6061 aaaaagcgat gactgcttta cttatcctaa aacaaattca atctggcaag ataacttttg
     6121 acacaaagct atctgaattt tatcctcaga ttcctggaag tcagcaaatc tcaatcaaaa
     6181 atatgcttta catgcgctct ggtcttcatc gaaccgcttc tccaaaaaaa cctatgtcag
     6241 atagtgagat cattcagttt gcccttcatc atttgaaatt tactggttat gatacgtatc
     6301 attatgaacc attaaattat acattgctta caggggtatt gatcaaacta atgaatcagc
     6361 cttatgaaac tcttttaaaa aaagaaatca ttcggccact ccatctagaa catacagatt
     6421 tttatgaaaa tgtaaaaaat tcccctcagc atgctgtatc ttaccaaatg tctgcaactg
     6481 acgattattc aaaagcattg gtcgagcctg aaacagacat ccgaaatgaa ttaggcacag
     6541 gaaatataag tatgtctgtt tatgacttga acaaattctt taccagtgtt ctgtctggcg
     6601 atattatacc aaaagaacta cttttctcat tgtggcaaaa taatggtgat aaacatcctt
     6661 acagcggcgg tgtttacagt ggaaatgatt atatccttgc tcagggaaat atcaatcgat
     6721 tccatgcagt agcggcgatg aaaaaagaca caaaagatgc aatcgtcatg gaaagtaata
     6781 tccaatctga taaaaacatc aaattgccgg ccactgattt aagaaatcag atttacgaac
     6841 tgatggaagg tgttaattta aaaagctaag tcgagacaaa aaaaccagca ggatgctggt
     6901 tttttgtttc aatcgatttc tgtctttaaa taacgttcca ttccgttcaa tatatcaaat
     6961 ttgggttgat aacctaaaga acggatcttt gaaatatccg ctaaagaatc acgaatatcc
     7021 cctgaacgct ccgcttcata tttaagcgct agctctgttc ctaagatttg atcaattgaa
     7081 tgaatcaatt caagtagcgt agtgcttttt cctgtaccga cattgaattg ctgtcctaaa
     7141 gctttttctt ccttagcaac aagtaaaagt gcttgtataa catcttctat ataaacgaag
     7201 tctctagatt ggctaccatc accaaataaa gtaaattctg ttttttctcc tgccaattgt
     7261 tttttgtatc ggtccactag aatcgaaata actccgctat atggagagtt aggattttga
     7321 tttggaccat aaacgttaaa aaatcggaca gcactagtcg gaacatcata taaatgacag
     7381 taatccaaaa catattgttc tgatgcaaac ttatcgatgg catatggtgt taaaggacgg
     7441 atgaccgatt cttctttttt tggcaacgtt ggttcatcgc cataaactgc agctgatgaa
     7501 ctaaatacca atcgttttaa accagattga tattttcgta ccaattcaag caataaaagc
     7561 gcactgtcaa aatttacacg atgagtttct agaggacgtt ctacagaatc tgccacacta
     7621 gctacagcag ctaaatgaaa aatataggca aactgatact gtttcatgat tctttccatc
     7681 aattgaggat ctgatacatc cccctcaatg aaagtcactt gtttctccat atccaagttt
     7741 tctttttttc ccattgataa atcgtcgact actacgattt tgttttcttt tcccaaataa
     7801 ttcgctagcg ttgaaccgat aaaaccagct ccaccagtaa tcaatatagt atccatcaaa
     7861 ttcactccaa ttatgcattc tcatacttaa taaagttagt ttaccatttt tatattgtaa
     7921 aaactataaa atttgttgaa gttgggataa ctagttttct tttctacttt atatacctaa
     7981 aaatagtttc aattagttac tccttcttct ttagatatag ttttttggat agaacccaga
     8041 tatccccaaa aaataaatga acctatcgta ctaaccaaaa tgagttctgt gataaataat
     8101 gccgaaactg ctattgtaag aatcattaaa atagcaaaaa agtgcttatc ttttactaga
     8161 tttggttgtg tgaaacaata acgcaaagtt ttaaagcatt ggataaataa gaaggttaac
     8221 aatgtcaata gacctaatcc tcctgtagca gccataacta agaaaataaa attatgagca
     8281 gttaaagtat cttttcccat taatgtatat ggtagtttat catgtgcgta ttcaatatag
     8341 tatcctggag aaccgccgga tatcggctta ctcttgaata tttcaattgc acttttccaa
     8401 agtccaaacc ttaaattaga aacatccccg ccatcagtgt cagggcgttt taaatcaact
     8461 ttattgctgc tagttgttcc tgatgaatca gatatatctt tttttatttc tgtaatatgc
     8521 ggaacatgtg cccatgcttg agcagttata tctgttacat aatacatcgc aattatactt
     8581 agcaatccta ctactaatgc agttagccat ctttttaaag tattagtttt aagaaaacga
     8641 ctactataac ttgtaaagaa actaaaaaca aaagttgcta ttaataaaca aactaatgtg
     8701 gtgcgtgagc ccgataaagc aataaacgag aattgaataa aaatattaat tgcaaaaaat
     8761 attttaaata gaagatttaa atgcttgtaa gtgaccaaat aatatatagc aataatgatg
     8821 acaattaatg atataaccgc accaaaattt ggatctgtat atacaccaaa taaacggttg
     8881 cctaaccacc ctaaacgaac tgggtaatac ttatatttta ttggaatttt tatgccatat
     8941 tgaatgacaa accagtataa tgatactaaa ttagctaaaa accataccaa tgaaagtatc
     9001 caagtaaata ctttgaaaaa aaagttacta ctgtagtctt tgccaaactg ataaatcata
     9061 aagaaaaaga ttaattgcca catgattaac ttgatatttt cgctatatcc atattttaaa
     9121 ttcattaaac tgcttactag catcgcgcca ataaaagcaa ttaatagcca atcatatttt
     9181 ttaagatttt tttctctaaa tgcaataatt gcatctataa tgatcaagac aactcccgct
     9241 attccagcag gccaaaaaag taaagtatca tatctagtag gtaatagtaa atacgggcct
     9301 attgctcgta acattagaat aaaaccaaaa taaataaaat accaaccttt cacatatttt
     9361 aaaaattttt cattcaacat ttttcctcca tccacctatt tctcataaac atttcttatc
     9421 ctttttagga taaactggtt ctttcaattc taacaaaaaa atgctaaact aacaatgtat
     9481 attaatgaat ttcgagaggt gaaagatgaa aagagtaatt acttacggta cctttgattt
     9541 gcttcactac gggcatatca atttactgaa acgagcaaaa caatatggag attatcttat
     9601 tgttgccctt tctacagatg aattcaattg gaatgcgaaa caaaaaaaat gttattttag
     9661 ttatgaaaaa agaaaacagt tacttgaagc tattcgctac gttgatttag ttattcctga
     9721 agaaaattgg gaacaaaaag taaaagatat aaaagaatac catgcagatt atttcgtgat
     9781 gggcgatgat tgggctggac aatttgatga acttgaacaa ctaacagatg cacaagtaat
     9841 ttatttacca agaacacctg agatctctac aactcaaata aagaacgaac ttaattcttt
     9901 aaatagtgat aaataatatt ttttaaacaa tgaaagaaca atatatctgg ataagacgta
     9961 aaaaaatggc tctataattt ctaggactga tagaattttc tgtcgatcct agattttctt
    10021 gtaaaaaaaa cagacataca aaaatttcga taaaatagag tcgtctaaaa catcaatctg
    10081 aaaggaagat tttgtatatc catttctcgt tctatcaaag agcacttcac tattaaagat
    10141 aaaaatattg aaattaattc ttttcaaaaa actttttata aggacaagaa agccatcgta
    10201 atcgaaggta cattagttcg cacttttaga cattgtctat tttgtaaaga aactgcgaag
    10261 cagctcgtga aaaacggcaa gaagatttca atgattatct taaatcgcag tgggaataaa
    10321 taaacctatt tgcgtcttag aaaacaacat taccactacc aatcatgcgg aaaatatttt
    10381 actgctcata cgtatcttgt cgttccttca tgttttattt cgaaacaagt tcattacacg
    10441 atattagaag agttgaccga gcgtcaagcg ttaaagacac ttacgaaacg ttgtgatctt
    10501 tcagtaacga catttcaacg gacattatct gctctatcgg caatgctgcg acagatgcta
    10561 gactatttac ctcattgttt actattcgac gagtttcgct ctctttcaat ttcccatgga
    10621 aagttctcct tttcttgtat gaatagagag acagggaagc tctttgacat tttgtccagc
    10681 cggagaaaaa aagatttgat tgcttatttc atgcgtttta caagaaaagc tcatatgggg
    10741 gttcgataca ttgtcacaaa tatgaatgcc ccttattttt cattagtcaa agagtccttt
    10801 ccaaacgccc aaatcatcat tgattgcttt cacattggac aacacttgaa caagtttttc
    10861 gatcgtgttc gaaaacgtgt gatgaagcag ttgaatcaaa aagacacttc acaagctaag
    10921 tactatcgac aattgaaacc tttgtataaa ttacttctaa aatcagatga cgagttggat
    10981 tacacaacat tcaagaaatg gcagaatttt caatggcgct atttaacaga aagtgaagta
    11041 gttgatcgtc taccctcaat ctcttatgaa cttaaaattg ctaagcagtt tatcaaaagc
    11101 tgcttagcaa ttatcacaag aaacagctag ctacttcgag aaacgaccaa agaagttccc
    11161 tcgaaacttc actatctcaa aaagcctttt tcatatctga aaaaggcttt taactagcat
    11221 tttcgaaaaa gtattctaat aacaaaatta gagaatcttc gcacacatat caaaacattg
    11281 aaacgatttg cttatgggtt tcgcagtttt accaatatgc gcttgtggat ttttctaatc
    11341 aacaggttaa tcagctatgc attaaaagct agtaactctt actaactttg ttgttcataa
    11401 atgaccttat cagcccgatt tgacaaagaa ctactttttt acaacattat tttattttcg
    11461 agtaatcttt cttatttcat ttcgtatatc tggaaaaaga aaataaacaa acggaagata
    11521 aataatgaaa atcattattg atttaattgc catatctaaa aatgaagaag agaataattg
    11581 tggaagaaag taataggccc aagtagtcaa agtaagattg actataaaaa ataagataac
    11641 gttcttccca aagaaatagg atatttttat ttcttttcgt acaaaaaata ctctaaagaa
    11701 aaagactaat aattctacta ccacgagtac gatcgtagcc gctacagcgc cataagtagg
    11761 cacaaaagct acatttccaa taatacttac aatcgcacca atgatcattg gataagcata
    11821 ctccttatct ttttctatag caattgcaaa ttggttagca aaaactccac caattggact
    11881 aacaactata attagacaag aaataagcat gtttattgtc atcggtttga aattctcccc
    11941 aaaaaaccat ggaacaaatt cttttgtatt gataaacacc acactaaaaa gaattaatgc
    12001 aatgataatc gtgtaatcaa aagatttttt tgtgtaaact aaaatatttt ctgatttccc
    12061 ctctgcctgt gctttagata ttttaggcaa cataacaata gtaattgatg ttaagactgc
    12121 caatataata cgagcaattt tttgagactg atcataataa gataattccg ttggatccac
    12181 aaaataccca actatcgttt tgtctaaagt tgtgtacact tgcataaaca actgaggagt
    12241 caaaataata aaagctggtt tccacaactg tttaaaaaca ttagagttaa agattggttt
    12301 acctactttt ccgtggatac ttaaccagaa aactaaactt cctaaaaagt tagtaattga
    12361 agtaatgaac acgtaaatcc acaaatcatc tttagttgaa acaaaaatga ttattaacaa
    12421 taccgatgat aatttgataa ctgtatttct aataaccact ttcgaaaatt cttcaattcc
    12481 ttgaaaaaac caagaaatat ctaatgttag ccctacaata aatatcaatt gaattaagta
    12541 taaattttta tactttacat caaaaaaata aatataggct atatagagaa aggaaacact
    12601 caagcctatt aaaaattgaa taaagtaaat attccagaat tgtaaagata gtgctttttt
    12661 atctttcctt gatttagcga tatttcgaat acgaaaagga ttcattccca cagcaatgac
    12721 agtcattaaa aactgtgata ttgagaaaac atacccataa atccctaaac cagttgaacc
    12781 aattctacga ctaagtatag gtatagtaag taagggtaaa acaatcaaaa agacttgata
    12841 tatcgcatta taaaatatat cttttactgt ttttttcact ttatatccct ctctatcaac
    12901 tcaagaacct tttctgtact tgctcctgtt tcataatttc caatataatc aagtaactct
    12961 tgagaacttt tcaaaagctt ttcttgttgg tttttaaaaa catcaacaag gccataacta
    13021 tcttgtacgc ttataattgg taactcactt aactgtatat aaaaacctct ttctaagcta
    13081 tattcttttt catcatagtt aaaaagaata attggttttt ttgttagcgc aaaatcgaaa
    13141 aaaatagatg aataatctgt gattaaaaca tctatctctt caaaaagcat ttcaagagga
    13201 atcccatcat taattaaagt aatttgatgt tttttcatat attctatact tttagaatca
    13261 gttgctaatt tactatgaac attaggatga aatttagtca taaaatttaa ttcttgttgt
    13321 tgctttttaa aaaattcacg taagttatcc acacctaacc attctagttg cgtaattccg
    13381 ttgtcctcaa tattatttcg aaaagttgga gcaaataaga cattgatttg tttctttttc
    13441 aagtcaaaca cttgctcttt actagaggct aattcatcgt ttctaggagt tcctacttct
    13501 aatattttag aaggaacgat attgaatgct gaaacatact tcttagaact atagcgatta
    13561 ccagataata aatatgtaat tgactcacta tcttttttcg catagcttag gtaacgttta
    13621 ggtaacttat cttttgcatc aaattcaatt ttttttaaag gcgttccgtg ccatgtttga
    13681 aaataaagtt gttttttcct ttttgcatac ttaatcgttt tccgactatc gtctatccaa
    13741 tacttagctg tatataaata aaaggcagct aaaaaagaat cggagtcaat tcttttaata
    13801 taatctggta aatcagttat aatcgcttct tttttgatta accaaataat tctgtaattc
    13861 aatttttttt catgaatttt ctctgaaata actcttggat ttccagaata gttacgcccg
    13921 ccaaaacttg aaaatacaat taaatttttt tgaataggta atattttaat aaaaaccggg
    13981 aaaatattcc ttgaaaaaaa tttttttata ttcttcatct cttacctcac agattctatc
    14041 ttaatcaaga atttacttgc tatcttacta cttgacatat agtaaggcaa gtttttattt
    14101 attaataaaa actttactaa tttttttaat tttggtgaaa agtcgggatc aattttcact
    14161 tctttttcaa agcgttgtaa aatatttata gaacgtaaat aatcttcttt attggtatag
    14221 ttatttttaa tcattgtcat catcatcctt attgcttcat agacatagcg agttctaaca
    14281 ttaattgata attccggata aaacttatct atgaatatta gtatatcttc atggacttca
    14341 aaagaatcaa aaattctagg attgaagctt cctctcagta tacttttagg gctttggtaa
    14401 tagtgatatt tagcagatga attgacagca actttttcgg aatgatcgat cagcaaataa
    14461 gtagtagcct tatcttccat taatttaccc agtgggaatc ttatattatc aaataaattt
    14521 ctattgaata atttattatt tgcagaatag ccaattttat tatttataaa taagtttttc
    14581 attgcttgtt ctctatcaaa tatttcccat tttctctcag caattgtatg ttcttttagt
    14641 tgttctaaac ttgaaatctt ttttattttt agcaccgaga tatctgctcc ataagtttgc
    14701 gtgccaaaat atagtaactc aagatagtct ggttctatcc aatcatcgct gtcaataaag
    14761 cacacaaaat ctcctgttgc agaatcaaga ccagaattcc tagccgcact taaaccacca
    14821 ttcacttgat gtatcacttt taatcgcata tctttctttt tatattcttc tgcaatttcc
    14881 ccacttcgat ccggggaacc atcattgact actattattt caatatttga atgtgtctgg
    14941 ttggaaatag attctaagca gttttctaaa tagtcttcta cgttatatgc gggtactata
    15001 acactaattt tatgcactct cttcacttcc taaaaatcat cttttttatt tttacaaatg
    15061 tat
//
LOCUS       DAZABD010000065        14127 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000065 DAZABD010000000
VERSION     DAZABD010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14127
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.065"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(13..3666)
                     /gene="rpoC"
                     /locus_tag="ACHALG_002187"
     CDS             complement(13..3666)
                     /gene="rpoC"
                     /locus_tag="ACHALG_002187"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFN2587557.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(3745..7362)
                     /gene="rpoB"
                     /locus_tag="ACHALG_002188"
     CDS             complement(3745..7362)
                     /gene="rpoB"
                     /locus_tag="ACHALG_002188"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFN2587558.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            8090..9058
                     /locus_tag="ACHALG_002189"
     CDS             8090..9058
                     /locus_tag="ACHALG_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN2587559.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            9076..9981
                     /locus_tag="ACHALG_002190"
     CDS             9076..9981
                     /locus_tag="ACHALG_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN2587560.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            9978..10784
                     /locus_tag="ACHALG_002191"
     CDS             9978..10784
                     /locus_tag="ACHALG_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN2587561.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            10941..11909
                     /locus_tag="ACHALG_002192"
     CDS             10941..11909
                     /locus_tag="ACHALG_002192"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HFN2587562.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(12226..12750)
                     /locus_tag="ACHALG_002193"
     CDS             complement(12226..12750)
                     /locus_tag="ACHALG_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HFN2587563.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            13037..>14127
                     /gene="scm"
                     /locus_tag="ACHALG_002194"
     CDS             13037..>14127
                     /gene="scm"
                     /locus_tag="ACHALG_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFN2587564.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTT"
BASE COUNT         4000 a         3049 c         2789 g         4289 t
ORIGIN      
        1 tttttaaaat atctattttg tttgattgtt cattgcgtct tctgcagcca tttgcgcttc
       61 aatatctgaa atactgtaga cattttcgct tgctacaccg acttcttttg gttccatgtc
      121 acgataacgt tgcataccag taccagctgg gatgatctta cctataataa cattttcttt
      181 caaaccaaga agaggatctt tcttaccgcg gatcgcagca tccgtcaaga cacgagttgt
      241 ttcttggaat gatgcagcag ataagaaact atttgtttct aatgatgctt ttgtgatacc
      301 tagtaagaca ggtcgagaag ttgcaggaac gcctcctgag actagcgtat tgtagttttg
      361 atctttaaag tctgcgatgt ctaataatgt acctggtaag atatctgtat cacctggatc
      421 cattacacga actttacgca gcatttgacg aaccattact tcgatatgct tgtcgccgat
      481 ttctacccct tgcatacggt aaacacgttg tacttcacga agcaagtagt tttcaacaga
      541 taatacatcg cgaacttgca acaattgttt tggatcgatt gatccttctg tcaatggtgc
      601 accacggtgg atcatatcgc cttctgctac tttcatacgt gctgtataag gaacagtata
      661 cgtacgtgta tctgtttttc ctttgattgt tacttctttg gtacgtgttg caggatcttc
      721 tgagatatcg atgacatccc ctgttacttc tgtgatcact gcttgccctt tcggattacg
      781 tgcttcaaag atttcttgga cacgaggaag accttgagtg atatcgtctc cggcaacccc
      841 acctgtatgg aatgtacgca tcgtcaactg agtaccaggt tcaccgattg attgagcggc
      901 gatcgtacca actgcttctc cgacttcaac gtcagagcca gttgcaaggt tgcgtccgta
      961 acaatgtttg cagacaccat gttttgtatt acatgtgaag acagaacgga ttgtcactgt
     1021 ttcgacacct gcatcaacga tttgttttgc gatgtcttcg gaaatcaatt ggtttcctgc
     1081 aatgatcaat tcatttgttt caggatgacg tacttcttta cgggtgtaac gtcccactaa
     1141 acgatcttct aatgattcga tgatttcatt tccctcgcgg atggcttgga tttcaagacc
     1201 tcggtcagta ccgcaatctt cttcacggat gattacatct tgggccacgt caaccagacg
     1261 acgagtcaag taacctgaat cggcagtctt caaggctgta tcggtcatcc ctttacgagc
     1321 cccgtgagtt gagataaaca tttccaagac ggatagtcct tcacggaagt tcgaaatgat
     1381 tggcaattcc atgattcgac cattaggagc ggccatcaaa ccacgcattc cggcaagctg
     1441 tgtaaagttg gaaatgttac cacgagctcc agaatcagac atcatgaaga ttggattttt
     1501 cgcttccaaa ccttccatca gtttttgttg gatgctgtct tttgcagcat tccaaacagc
     1561 aatgacacgt tcataacgtt cgtcatctgt gatcagacca cgacggaatt gttttgtaat
     1621 agtttcaact tgtttatggg cattttcaat gatttcttgt ttttcattca gtacactgat
     1681 gtcagcgatc cctactgtga taccggcatg tgtcgaatgt ttgtacccta agtctttcat
     1741 acggtcaagc atcttagaag tttctgttac tttgaatcgt ttgaagactt ctgcgatgat
     1801 gtttcctaag tttttcttct tgaacggtaa tactaattct tgctctttga tatgtgcagg
     1861 aatatcagta ccagcttcaa caaagtattt gtccggtgtt tggacggtca agttgaagtc
     1921 tgttggttca ttcagataag ggaattcagg aggcatgatc tcgttgaaga tgatcttacc
     1981 aactgttgtg atcatcgtac gttctttttg ccattcagta aatggttttt cacctaatga
     2041 gttagggttc acaccgatac gtgaatgtaa atgaacatat ccgttgcgcc atgcgataac
     2101 ggcttcgttt tgatctctga agaccattcc ttcgccttcg cgtccttctt cttccattgt
     2161 caagtagtag ttacctagaa ccatatcttg agaaggagta acaactggtt taccatcttt
     2221 agggttcaag atgttttgag cagctaacat caacatacgt gcttctgctt gtgcttcttc
     2281 attcaatgga acgtggacag ccatttggtc tccatcgaaa tcggcattat aagcttcaca
     2341 tacaagtgga tgcaaacgga ttgcgcgtcc ttgtactaaa actggttcga atgcttggat
     2401 accaagtcgg tgaagcgtag gtgcccggtt cagcaatact gggtgttcct tgattacgtc
     2461 ttctaaaaca tcccatactt cgtcttcttg acgttcgatt ttacgtttcg catttttgat
     2521 gtttgaagct agttcgcgtt gaacaagctc acgcatcacg aatggtttga ataattcaat
     2581 ggccatttct tttggaagtc cacattggta catcttcaag aaaggtccaa caacgataac
     2641 ggaacgacct gagtagtcga cacgtttacc tagcaagttt tgacggaaac gaccttgttt
     2701 ccctttcaac atatgagaaa gagatttcaa tgggcggtta cctggtccag taactggacg
     2761 tccgcgacga ccgttatcga tcaatgcatc tactgcttct tgcagcatac gtttttcatt
     2821 ttgaacgatg attccaggtg cattcaagtc taacaaacgt ttcaaacggt tattacggtt
     2881 gattacacga cggtaaagat cattcaagtc acttgttgca aaacgtccac cttctaattg
     2941 aaccattggg cgtaaatctg gtggaataac agggataacg tccataacca tccagcttgg
     3001 ttgatttcct gaagcgcgga atgcttctaa aatatccaaa cggcggattg cacgtgtacg
     3061 tttttgtcct tgtgcagatt tcaattcttc tttcaattcg gctgcttctg catctagatc
     3121 aacattatca agtaattgtt taatcgcttc tgcacccata gcagcttgga attcttgtcc
     3181 atattgttca cgtttttcgc gatattcgcg ttctgtcaac aattgttttt tctctaaatt
     3241 cgtatttcct ggttcgatga ctacatatga agcaaaataa atgatttctt ccaaagcgcg
     3301 tgggctcatg tctaatacaa gacccatacg tgaaggaatc cctttaaagt accagatatg
     3361 tgaaacagga gctgctaatt caatatgtcc catgcgttca cggcgaactt tcgaacgagt
     3421 aacttccact ccgcatcgat cacaaacgat tcctttataa cggatacgtt tgtattttcc
     3481 gcaggcacat tcccagtctt ttgtaggacc aaaaatccgc tcacagaaca acccttcgcg
     3541 ttcaggtttt aaggtacgat agttaatcgt ctcaggtttt tttacttcac cgtaagacca
     3601 gcttctgatt ttttcgggag aagccaaacc tatttgcata ctttcgaatt tatttacatc
     3661 gatcaaaagg ggttccctcc atttcattta atggcaaacc gcaagacggc tgatcttcga
     3721 ccgccttgca gctcatagac cggattagtc taaattatct tctgcggttt caaagttttg
     3781 gatgacatcg tctttttcat cttccacttg ttcagcagct ttcttttcta attctttggc
     3841 agtttgctgt tcggcaaatt ttgtcaaagc atcaacagtg atcaaatcgt cgtcttcatc
     3901 gtccatgtcg cgaagttcga tctctgtttc ttcgatatcc aagacacgca tgtccagacc
     3961 tagtgattgc aattctttca ccaatacgcg gaatgattcc ggtacacctg gttttggaat
     4021 tggttcaccc ttgacgattg cttcgtatgt tttcacacgt ccgactacgt catctgattt
     4081 gtatgtcaag atttcttgta aagtataagc cgcaccgtaa gcttccagtg cccatacttc
     4141 catttcacca aaacgttgtc caccaaattg agctttacct cccaatggtt gttgtgtaac
     4201 caatgagtaa ggtccgattg aacgtgcatg caatttgtca tcgaccatgt gagcaagttt
     4261 gatcatgtac atcacaccga cagacacacg tccatcaaat ggttcacctg tacgtccgtc
     4321 gtacagaaca gttttcgcat cgctggccat accggcttca cgaactgttt cccatacgtc
     4381 ttcatcactt gcaccatcaa atactggcgt tgctacgtgg atacctaact gacgtgcagc
     4441 catacccaag tgtaattcca atacttgccc gatgttcata cgtgaaggca cccctaaagg
     4501 attcagcatg atatcgattg gtgttccatc aggtaggaat ggcatatctt cttccggcat
     4561 aatacgggat acaacccctt tatttccgtg acgaccggcc attttatccc cttcatggat
     4621 tttacgtttt tgaacgatat aaacacgaac taacatgttt acgcctggtg acaattcatc
     4681 tccagcttca cgagtaaaga ttttcacatc gtgaacgata ccgccgccgc catgtggtac
     4741 acgtaatgat gtatcgcgga cttcacgagc tttttcgcca aagatcgcat gcaatagacg
     4801 ttcttctgca gataattctg ttaccccttt tggcgtgact ttccctacaa gcaaatcgcc
     4861 atcttggact tctgcaccaa tacggatgat acccatttcg tctaaatctt ttaatgcgtc
     4921 ttccccgaca ttagggattt cgcgtgtgat ttcttcaggt cctaattttg tatcgcgggc
     4981 ttctgattca tattcttcaa tatggataga agtgtatacg tcatctttta ccaaacgacg
     5041 gctcatgatg atcgcatctt catagttgta cccttcccaa gtcatgaaac caactaaaac
     5101 gttttggcct agagccattt cgccttgttc catagatggt ccgtctgcca gagtatcccc
     5161 tttttcgact ttctcaccca aatgaacgat tggacgttgg ttgtagcttg ttccggagtt
     5221 tgaacgacgg aattttgtta catcgtattt atctaatgca ccgttgtcgc ggcgtacgcg
     5281 gatttcagaa gcatcaacgt attcaacgac accgtcatgt ttacataaca aagcagctcc
     5341 tgagtcatgc gctgatttgt attccatacc agtaccaacc catggtgctt gaggattgat
     5401 caacggcact gcttgacgct gcatgttggc acccatcaag gcacggttgg agtcatcgtt
     5461 ttccaagaaa ggaatacatg ctgtcgcaac ggcaactact tgtttaggtg atacgtccat
     5521 gtagtctact ttatcaatag atacttctaa gttttcactt tgagcacgag ccataacaac
     5581 gtcttgagca aatgttccat cttcattcaa tgggctgttt gcttgggcta cgatgtaatg
     5641 gtcttcgata tcagctgtca agtagtcgat ttggtcagta acacgacctg tttcacggtc
     5701 tacacgacga taaggtgttt cgataaatcc gaatttattt actttcgcat aactggacaa
     5761 actattgatc aacccgatgt ttgggccttc aggagtttcg atcggacaca tacggccata
     5821 gtgggagtag tgaacgtcac gaacttcata accggcgcgg tcacgagtca atccgccagg
     5881 tcctaaggct gaaagacgac gtttgtgtgt caattcgcca agagggtttg tttgatccat
     5941 gaactgtgac aactgagaag aaccaaagaa ttctttgata cttgccacaa ctggacggat
     6001 attgatcaat tgttgaggtg tcaatgtttc agtatcttgg atcgacattc tttcacgaac
     6061 cacacgttcc atacgtgcta gcccgatacg gaattggttt tgtaaaagtt caccgactga
     6121 acggatacga cgatttccta agtggtcgat atcatcgaca ttgccgatac cttccatcaa
     6181 gttcaagaaa tagctcattg aagcaatgat gtcggctgga cgaaccgttt ttacagctgc
     6241 ttctggatag ccgtttccga tcacgtttac ttcacgttca gggtctcttg gtgaaaatac
     6301 tttgatgact tggacagtca ttggatctgt gaccacaccg tcttctgatg gatagtaagt
     6361 cactgaattt aggccattat caatgaaagg agctagtgtt tccatgactt ggtgagtcag
     6421 aactgttcct ttttcaacga tgatctcgcc tgtttctgga tcaactaatg tttcagctaa
     6481 tgtcagattc aatagacgtg ttttcaaatc taattttttg tttactttgt aacgaccaac
     6541 gtttgctaag tcgtaacgtt ttggatcgaa gaaacgagca ttcagcaagt tacgtgagct
     6601 gtctgctgtt tttggttcgc ctggacgcaa acgttcgtaa acgtctttca aaccttcttc
     6661 tgttcttgag tcgcttgcgt ttttatgcaa gtctttttcg actgtattgc gtaatgtttc
     6721 gctgtcgccg aagatttcaa agatcgtatc atcggaacca aatcctaacg cacgaactaa
     6781 tacggttaaa ggaatcttac gtgtacggtc gatacgtaca taagagatgt cttttgcatc
     6841 agtttccatt tctagccaag ctccacggtt agggataaca gtagaaccga atccttcttt
     6901 tccgtttttg tcgacttttc catggaaata gacacctggt gaacggacta actgagaaac
     6961 gattactcgc tctgccccgt tgatgatgaa tgttccttgt tctgtcatta atgggaaatc
     7021 accgaagaat acttcttgtg ctttgatttc gccagtctcg cggtttgtca aacgcaatgt
     7081 tacatgtaat ggtgcagagt agtttgcatc atgcgcacga gcttctgcta ctgtatactt
     7141 tggttctttc aattcataat ctacaaattc caaagataga ttgccattga agtcatcgat
     7201 tggcaaaatg tcttcaaaca tttctctaag tccctcatct aagaaccatt ggtaagagtc
     7261 tgtttgaatt tcgatcaaat ttggtaattc caacacttca ctgattcttg cgaaacttct
     7321 acgttcgcga tgttttccgt attttactac gtgtccagcc aagctcttca cccctctaaa
     7381 atgtcattca aaaagactac ccgatcaatt gttacaataa tatttcaaat attattttta
     7441 ggtaacaaca gacatttttt gcggctttta ggcaaaaaaa aaccaaaaat agaacattct
     7501 cttgaaacct tacttcgtga gcttctactt ttgtttcctt atattaaagc cgaaacggac
     7561 aatatttcgt ttctgccttt tcttcatcag atagtttttg caacttcata tactacactt
     7621 attttctata aaagtcaaca cttcctggcg tttcttctgc tttttcgcaa cattttctta
     7681 atgttttcaa ttttatgttt tcaattatct gacaattctt aattgacaga cctttttatc
     7741 tcggttatac tttagaaaat tcattttgtg gaggaacttc tatgcgaacc aacatcaacc
     7801 aaatcgcacc aacacaactg aataactttt actttagcta ctttagatag gtttgtattc
     7861 atcatgatgg atacaaacgt taaacgacag tttgtatcta tgatggctaa agaactttgt
     7921 attgcagcaa aaagccacgt agacaaaatg ctaagtggct gagcagtgaa aatacaaagt
     7981 aaaagtatca agcattctac tttttcaatt tattttccga cttttcaatc catttggaca
     8041 ttttgtctga atggattttt tgtttcccaa aattaaggag gaaaaagaaa tgaaaaaaaa
     8101 tataatcatc ggattaggcg ctgccctgct actagcttta ggaggttgcg cacaaaaaga
     8161 caattccgca gaggcatcag atggacaaaa aacaattggc gtattgcaac ttgtacaaca
     8221 taactcgtta gatgctgcct ataaaggatt caaagaagga ttagcagaag aagggtataa
     8281 agaaggcgat aatctaacaa tcgattatca aaatgcccaa aacaatcaag acaacctgaa
     8341 aagtatgagt gagaaactcg tcaaagaaga tcctgatttg ctacttggaa tcgccactcc
     8401 agcagcacaa tctttattaa atgaaacaac cactatcccg atgactgtca cagcggtcac
     8461 agacttggaa caagcccatt tagtcacatc caatgaaaaa cctggcggca atgtgactgg
     8521 cacgacagat atggtgccaa tcgaaaaaca aatcggctta ctgttaaata tcgtgccaga
     8581 cgccaaaaca atcggcatca tgtataacaa cggtgaagca aactctaaga tccaagctga
     8641 tttggcagaa aaagaattga aaaaagcggg cgtcaaagtc aaagtactga cagcaaattc
     8701 cacaaatgat gtccaacaag tgacgaaaag tctagctaaa gatgtagatg gaatctatat
     8761 tcctactgat aacacgttcg caagtgcggc aagtgttgtc ggtgaagtcg caaaagaaac
     8821 aaaaacacct gtcgttgctg gcgcagttga tcaagtcaaa gacggcggtc tagcaacagt
     8881 aggaatcgac tacgaaagct tgggcaaaca aacagggatc atggcagcaa aaatcttaga
     8941 cggcaagaat ccaagtgaac ttcctgtcga aacagcgaat gatttggaac tgtatgtcaa
     9001 taaagacatg gccaaagcat taggcatcaa tccagacagc atcaaagaag ctgaataaaa
     9061 cacaggagga ttctcatgac aaatttgctc tctcttttct tatcatctgc atcgcaaggg
     9121 gtcttatggg ccctacttgc gattggtgta tacgtcactt tccgtatctt ggacattgcc
     9181 gacttgactg ctgaaggaag ctttccttta ggtgcaggga tcgcggcagt aagtattaca
     9241 aatggctatc atccattgac agcttgtctt ctaggctttt tagggggtgc ggcagcaggg
     9301 cttgtttccg gactgcttca cacaaaattg aaaattcccg cccttcttgc agggatcatc
     9361 accatgactg gtttatattc tgtcacatcc cgtgtgatgg gtgcgccgaa catctcttta
     9421 ttgggtcagc caacgatctt tcattgggtc gagtcattcg gcataacaaa agacaacgct
     9481 gtactgatcg tcggtctgct ttttgctttg atcgttgtat gtttacttgt tttgttctta
     9541 cgtacagaaa caggacttgc tattcgtgca actggcgata atctggcaat gagcgaagca
     9601 aacgggatca acacagataa tatgaagatc atcggttata tgatttctaa tggatgcatt
     9661 gctctttctg gctccttact tgcgcaaaac aacggctttg ctgatttaaa ttctggtatt
     9721 ggaacgatcg taattggact tgcttctatt atcatcgcag aagttttgtt acgtaaccag
     9781 ccactatttc tccgtctctt gactgtcgtt ttaggtgcgg tcatttatcg ttttatttta
     9841 gcgttagtct tcgaactgaa tgttgaacca agtgattcga aacttgcttc tgctttcgtg
     9901 ctagtgatct gtctttcttt cccgcagatc cagcaaaaat tccatttacc tcaaaaaaag
     9961 aaaggcggta gtgtcaaatg aatgacgtct taacattgga aaatctccat catacctttg
    10021 aaaaaggaac gatcaacgaa aatcatgtcc tgaaaggcat taatctttcc ttagatcccg
    10081 gcgacttcgt gacgatcatc ggtggaaatg gggctggtaa atctactttg ctgaacagca
    10141 tcgccggtac attttctgtc gatcagggtc aaattttatt gaatggaaaa aatatcacga
    10201 aaaaatctgt tgtcgaacgt tcgaaatcca tcagtcgcgt attccaagat cctaaactag
    10261 ggacagccgt tcgtttaacg gtagaagaaa atcttgctct tgctatgaaa cgaggaaaaa
    10321 aacgcggctt cttccgtggt gtcaaaccac aagaccgaac atttttcaaa gaccagctgg
    10381 ttcggctaaa tttggggttg gaaaatcgtt tatcctcaga aatcggtttg ctgtctggag
    10441 gacaacgtca agctattacg ttattgatgg ccacattaca gcgtccagaa ctgatcttat
    10501 tagatgaaca cactgcagcg cttgaccctg ctacttccaa aacggtgatg gaattgacag
    10561 aaaagctgat cactgagcag aatctcactg cttttatggt cactcatgac atggaagacg
    10621 cgatccgcta tgggagtcga ctgatcatgc tgcatcaagg aaggatcgtc gtagatatcc
    10681 agcaagagga aaaacagcag ctcacagtgc cccaattatt agcgttgttc aaacaaaaca
    10741 gcggcacggc attaaaagac gatcaagtac ttttatcttc ataatcagaa agaggatggg
    10801 acaaaaaatg aacgcccacc ctcttctttt ttctctttcc ttctttcgtt tttgctttat
    10861 gtatcaaccg ttgtttcttc gaaaactatt ttgttctctc cttcttcctg atagaatgga
    10921 tgggaaagga gcttgatgcc atgacaacga aagacaaggt acttcaatta ttgaaaaaga
    10981 caccagattt tctttccgga gaaaagttgg cacagcaact ggaagtttcc cggacaagta
    11041 tttggaaagc catcaaagaa ttggaaaagg caggctatcg ttttgagcac gggcctacag
    11101 ggtatcgtta cctgccctct gacgtactag atgcaaaaga aatccagcaa gagctacggt
    11161 cgtttattcc tgatttgaaa gtacaaacga tgttgacttc tgaatctacg atgaaagatg
    11221 ccaaactggc agcagctcaa ggagattctg tttcgaagtt atttgttgct gatacacaag
    11281 aacaagctcg tggacgtttt ggacggcctt ttttcgctga acctggacga ggaatctaca
    11341 tgagtctctt gctccatcca gacaaacggt ttgatgaatt gcctcaatat acgatcatcg
    11401 cagccagtgc ctgtgtgaaa gccattgaat ctgtaaccgg tcgttctgct tcgatcaaat
    11461 gggtcaatga tatctacatg gatggaaaaa aagtctgtgg gattttatct gaagcagtca
    11521 gtgatatgga aagcggcagg atcagtcata tcatcattgg gatcgggctt aatttttctg
    11581 ttcctcaaaa tcattttcca gctgatctcc aagaaaaagc catctctgtc tttcccgacg
    11641 gacaagctag tgtcacaagg aatcagctaa tcagcaaaat ctggcaagaa ttgttcactt
    11701 atctttctca cccagaaaaa gcgttggact tctataaaga aaaatcattc gtattgggaa
    11761 aaaccgtgtc gttcaatcaa aaaggaacga cttatgtcgg tcttgcaaaa gaaatcggtg
    11821 atcatggcga actgatcgtc caaacaaatg aacagaagat gagtttgtct tctggcgaaa
    11881 tcagtctcac atctatccgt acgcaataat tatcagattt cacaaaagga cacctgatct
    11941 catacagtgt ccttttttct gaattctctt caaaagactc tccttattct atcagctgac
    12001 agtttctcta tcctctagaa aaaggtattc taaaaatatc tataaagcat ggtacacttt
    12061 ctttaaatga atcaatgcaa aaaacaagag agtcatcagt ttacacgatc aaaagagata
    12121 gcaggccgct tcctttttca atcttataga tagaccatgg gtaacgataa aaacaaaaaa
    12181 gaacttgcga cttattttgc cgtaagttct tttttagcta gtgccttaat ctcttaatcc
    12241 caacaaacgt ctcgcataga tcacctgacc agagtgcata gcagcatcat caataataga
    12301 aacgagacgc attcctcgtg tgactggtgg tgtccagttc tcgtcaatta cttcatctag
    12361 ctcggaaggt tccagccttc ttaagtaaga aatagcaaaa tcagcagctc ctcgcaagta
    12421 tcctagcaga tcttctattt gattagcttg gatctgcttt gcttgttcca gactatgctt
    12481 ccaatcttgt tcgttatctg ctatcgtcat cggaaaccgg ctttcccagt cttgactatg
    12541 ccaaattgga gaagttcctg ctaaatcagc aatctggaaa tctaattctc gagcggtatg
    12601 ccaagccagc caagtcattg atttgatctg cggggctcta tcttccactg gaaaacgatt
    12661 ggcctcctca atagagacgc cttcaaacaa acgttcaaac cgatcgtatg cccgatccac
    12721 actctcgcat agcatatcta aataatccat ttattttcct cctttttatt tatcctactt
    12781 actttttcct aattctagga tacctactta tatgttacta cacaccctta ttttttacac
    12841 tttaacagtc accatcatca atagtttgga gattgaaaga aaaagatagt gtcgaactat
    12901 caattgaaca agaagcaaca atatgaacgt ttcattaata ttctttaaat aaacaatgat
    12961 ttttcaaacg aacagtttaa gactaggaga aaaagagtat aataggaaaa tgtttgaaag
    13021 gaaaggaagg ggaaaaatga aaaaaagggc aagtttacta gtcctagttg ctttgttgtt
    13081 caatatcttt tcgtatggtg tggccgtttc agcggaaaca tacattccta aagaccctgg
    13141 gaatatttat aatgatctaa ctggtaacta tggcttgtta ggaattgcta gtcaattcca
    13201 cgtatttgct aaaaataaga caactgtgaa tgcacataca gacggaaata tagcaacaca
    13261 tgaacttgat gcaaaaaata attttggtac agagcttcat gaaggtttat tgaaccaaga
    13321 aataaattat atacaaaaaa ctgattcttt cgctaattct tcaggcatac ctacaggtag
    13381 cacaagaatg aataaatttg tcgttggcag taacgtggat gtagcacttc aagatggtca
    13441 tcctacagtc aatggatcca ggcttgatca cttaactcca gatgattttt ataaagatcg
    13501 tgctgggcat gaatatattg atttcgagca ggaatttgct aaattgaatg tagcagcaaa
    13561 tgatcttgca acgatcactc cagctaaaac atatactgct gcagattttc cagacatgaa
    13621 caatcggaca atcgatttaa ctgatttatc agataccggg tttttattgg taaatatcga
    13681 tgcagaagtg ttaacgatga atacgccact tcaaatcatt aatccaaatg atcaagtagt
    13741 tgtatttaat gtcatcaaca gttcttctgc attgaacgta cagtcaccaa tcaaatacaa
    13801 tgatcgttca aatcatgaaa cagaagattt ttcggatgcg aatatctctt ggaattttgg
    13861 aaatgagatg actgatttga caatcagtgc tccttttcaa ggaacaatcc ttgcgcctaa
    13921 tgctactatc cgcattactc aaaaccaaga cggaacgatt ataggaacaa atgtgatttt
    13981 agatgcagct accaatcgtt gggatcctaa cgaaatattt atcagcgaca atggtacaga
    14041 tacgacagac acaagcgaca gtacagatac atctaccaca agtgatagct cagattcatc
    14101 aacttcaagc gacagtacag atactac
//
LOCUS       DAZABD010000066        14118 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000066 DAZABD010000000
VERSION     DAZABD010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14118
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.066"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..556)
                     /locus_tag="ACHALG_002195"
     CDS             complement(<1..556)
                     /locus_tag="ACHALG_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HFN2587565.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGA"
     gene            complement(572..1051)
                     /locus_tag="ACHALG_002196"
     CDS             complement(572..1051)
                     /locus_tag="ACHALG_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFN2587566.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKARLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(1057..2081)
                     /locus_tag="ACHALG_002197"
                     /pseudo
     CDS             complement(1057..2081)
                     /locus_tag="ACHALG_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            complement(2098..3204)
                     /locus_tag="ACHALG_002198"
     CDS             complement(2098..3204)
                     /locus_tag="ACHALG_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFN2587567.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(3433..6030)
                     /gene="adhE"
                     /locus_tag="ACHALG_002199"
                     /gene_synonym="adhC"
     CDS             complement(3433..6030)
                     /gene="adhE"
                     /locus_tag="ACHALG_002199"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFN2587568.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(6335..6715)
                     /gene="yajC"
                     /locus_tag="ACHALG_002200"
     CDS             complement(6335..6715)
                     /gene="yajC"
                     /locus_tag="ACHALG_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFN2587569.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(6812..7957)
                     /gene="tgt"
                     /locus_tag="ACHALG_002201"
     CDS             complement(6812..7957)
                     /gene="tgt"
                     /locus_tag="ACHALG_002201"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFN2587570.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            8175..8873
                     /locus_tag="ACHALG_002202"
     CDS             8175..8873
                     /locus_tag="ACHALG_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFN2587571.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(9041..11680)
                     /locus_tag="ACHALG_002203"
     CDS             complement(9041..11680)
                     /locus_tag="ACHALG_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFN2587572.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(12299..13330)
                     /gene="queA"
                     /locus_tag="ACHALG_002204"
     CDS             complement(12299..13330)
                     /gene="queA"
                     /locus_tag="ACHALG_002204"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFN2587573.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            13523..>14118
                     /locus_tag="ACHALG_002205"
     CDS             13523..>14118
                     /locus_tag="ACHALG_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN2587574.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEA"
BASE COUNT         3959 a         3096 c         2543 g         4520 t
ORIGIN      
        1 aagcgcctac gagataacct cccataaaca aagcagagat cgaagcacca ccagcaacaa
       61 gtgaatacat gataaaagca gtacttggag gaatgaccat acctgtcgga gctgacgcga
      121 tattgaccgc tgtcgcaaac ttccggtcat atccttcttc cacttgctga gggatcaaca
      181 ctccaccaat cgcggtagaa gcggcaatgg ccgagctaga gatggaacca aataaagcat
      241 tccctaacac attcgtatga gccaaagccc ccggcacgcg cccgacgaac aacatcgcaa
      301 aatccactag tttcttagct atccctccat tattcatgat aatgcccgag agaacgaaaa
      361 acggaatagc gatcagcgaa aaactattga tacttcccac cattttttga gctgaactaa
      421 aagcagaaac atcaaacggt ataactaaca ataaagtcat catcgatgag aatgcaatac
      481 ttatagcaat cggcatccct aatacaagca acacaacaaa tacaaaaaat aacactaatc
      541 cggcttctat tgccatttta ttcctccatt tttatatttt ttcttgaatt gctggcttgt
      601 tgcttaatgt atcgattaga ttgatcaggc tatagataat gatgagtacc cccgatacag
      661 gtactgacag ataaacatat cccataggaa tacttaaaga aggcgagatc tgccccattg
      721 tcaatgtgac agcattccat ccgccataca ccatgatcat cgcagcaaat accagaaata
      781 aactttgaac aacaacttct aaaattttac tttttttgcc agttaatttt tcacttaaaa
      841 ccgttacggc taaacgtgct ttttgtccca caacatacgc ggaagacaac atggcaaacc
      901 agacaagacc gaatcgaaca agctcttccg tgaccgtatt tggatttcct aaaaccgttc
      961 tggaaaatac ttgccataaa acaacagcga ccatcccaac aagcaaaaca gctcccagga
     1021 tctctaatac cttgtttaaa atcattttca ttacagctac gctccttgct cttggatctt
     1081 ttgataaagg tctttgtagg taggatcgtt tttgaatata tcatgaagtg gggcaactgc
     1141 ctctcgaaac ggtgtattat ctacatcatt gaattggact ccatgttctt cttgtgctaa
     1201 agctttttct tcttcaattg cttttgcaaa agcttcaatt tcaaaaaccg ttgattcttt
     1261 cgctgcttct tcaatggctt tttcctgttc atcagttagc tgttctttga tggcatcatt
     1321 catgatgatg atatctggca cacgtgtatg ctcatcatag gaatagtatt tagctacccc
     1381 gccatgtcca gctgtataca agacaaattc attgttttct gatccgttga tcaaattgga
     1441 ttgaagagac gtgtacactt catcacttcc cataggtacg ggcgaaccgc ctaaaagctc
     1501 taccattttg atagcggttt cgctttgcat cacacggatc ttctttccct tcaaatcttc
     1561 cggcgtatgg atcggaccgt ctaccatata aaagctgcgc tgaccagaat cataataggt
     1621 caatccggta aatcctaaat ttttggtcga ttggtagatt ggctccatta tttcttcatt
     1681 atccatcacc ttaaagaaat gttcttcatc attgaataga taaggaacgg aaaaaatcga
     1741 ataatcttta gagaaacttt ccaacgctga ggcacttact tttgtgaaat ctactgctcc
     1801 tgactgtgtc aattcgatca gttcacgttc tccacccagt tgtccatctg ggaaatactg
     1861 gatctgtacg gaaccatctg ttttttcttt tactaaacgc ccaaattcat tcattgcatc
     1921 aattactggc tgttgcttgc ataagcaaag cgcaaggtaa ctatctcatt tacgtctgca
     1981 tctgcgacag ttgccacact agaattattt ccgcagccgc ttagacctag cagacctagg
     2041 ctaagcgaac ttgctgcaaa cagcagtgat gtttttttca tatgggtttc ctttctttta
     2101 acaataagag acgagcaatt ctgtcaaaca aagcaccgtt aacgactgtc cataaggcat
     2161 tgctgtgatt ccgatattcc gataaaaatc taaactgtcc cccattcctg tcccgattga
     2221 aacgttttga acttctcctt tttcatcgat ttgttcaagc aatccttgga tagcacgata
     2281 agcgacttct ttatattctt gtggtaaata acgtttatgg accgctttca aaataccgta
     2341 agcaaatcct gcagttgcag aagattctac gtaagaagaa ggatcatcca acaacgtatg
     2401 ccataaaccg ctttcagact gataagtttt taatgcacgc acttgtgctt caagtgtttc
     2461 gatcaataat ttgcgcaatg catcttcttt cgataattct aagatttcaa tgatctctgg
     2521 aatagcaatc gtcaaccagc agtttccacg agcccaaaat gcttcggcat agttatgatt
     2581 tccttcaaac gtccagccgt gataccacaa tcctgttcgt ttatccatca agtatttgat
     2641 atggatcata aactggtaac gagcttcttc taaatattct gggcgattca acagtttccc
     2701 gatttttgcc aaaggaagca ctgtcatcat caatgtatcg tcccacaact gattcttatt
     2761 ttccggtcca tatgtggcat gctgtaaccc gtcttcattt gtccgaggca tgtcattcat
     2821 gacccattct gcccattgtt ctaaataagg caaatagcgt gaatctttcg tgtcttcata
     2881 caagtaagcc atagtcaata gcggtgccat tgtattgacg tttttgggcg gagcgccttc
     2941 ttccatacga tcctcaaacc attcattgac cacttgataa gcgcgaggat tttttgtcat
     3001 ctggtaattt ttataaatac catatagacc gacaccttgt ggccaattcc acactgtcca
     3061 gcttttgtca tctactttca gtccatcaaa gtccaacaaa aattcaccat tatcgtcttt
     3121 gatcgttgtc aggttttcaa cgagcaggtc aattttttct ttcactaatt ctttaggtac
     3181 aaaaatctct tctttaataa tcatctttac gccacctttt taaacgcttt cattttatat
     3241 ccttaatata gcagtaatac cgtccactat aaatatcgct ttttgaacaa aaactgtcta
     3301 aaattgacct aaatgttaaa taacaaaagt aactttcagt tacttggaaa ttaaaaaaag
     3361 aacttggaga aacaacgttt tccaagttct ttttcatcta ttttcttatc ttttttctac
     3421 gctttctttt gcttatccgt tgtaagcatc aatgatgatc tgtttcaatt cagagatcaa
     3481 tggttctttc ggattagctg tcgtacattg gtcttcgtaa gctaattcag ccatacggtc
     3541 tacggttgta tctagtgttt cttgtgtgac tccttgagct tttagactca tgttgatgcc
     3601 gacagatttt cctaattcat aaacagccgt tgctaaagct tcgactaatt cctctgttgt
     3661 ttcgcctttc agacctaaga attttgcgat atctgcgtaa tctgtatctg cacggaaata
     3721 gtcatattta gggaacatcg catgtttttg tggatctttt gcattatagc ggataatatg
     3781 cggtaaaaga atcgcatttg tccgtccatg agggatcccg tattccccgc cgattttatg
     3841 tgcgactgag tgacaaatac ctaggaatgc gttagcaaac gccataccag ccattgtaga
     3901 agcgttgtgc attttttcac gtgattccat atcaggtgtt tttactgatt tttctaaata
     3961 gtcaaagact aacttgattg cttgtaggct caatccacgt gtataatctg aagccatgac
     4021 tgaaacatag gattcgattg catgtgttaa cacatccatc ccggtatctg ctgtgacaga
     4081 tgctggaact gacatgacga attgaggatc gacgattgcg acatctggcg tcaatgcata
     4141 atctgctaac ggatatttca catgtgtttc actgtcagtg atgaccgcaa atggcgttac
     4201 ttcagaacca gtccctgatg ttgtcgggat acatacgaat tgtgtttttt ctggtttgtc
     4261 gatcttatat gtccgtttac ggatatccaa gaatttttgt tttgcaccaa agaaagatgt
     4321 gtctgggtgt tcatagaaca tccacattcc cttagctgcg tccattgcag aaccgcctcc
     4381 aagagcgatt actgtatctg gcttgaagtc tgccatcagt ttcgttcctg cgtatacggt
     4441 atttgtcgat gggttcggtt caacatctga gaagacttcg atcttcacat catttttacg
     4501 tcgtgccaat acttcacgaa ctgtatcgca gtaaccgaat tggaccatac cagggtcaca
     4561 aacgatcatg acacgttcaa cgttttccat tttctctaag tacaatagag agttcttttc
     4621 aaagaagatt tttggcggta atttgaacca ttgcatgtta tttctccgtt tcgctacagt
     4681 tttgacattg attaagttta cagcagagac gttacgagaa acagagttct taccataaga
     4741 accgcatccc aaagtcaatg acggaatcat ttcgttatag atatcaccga tgccgccttc
     4801 tgcagatggt gtattcacta aaatacgaca agctttcatt cgaagaccga atttgacatg
     4861 caactcttcg ttttctgaat ggataactgc tgtatgtcct aatcctccaa gattcaacat
     4921 accttcacac aaatcaaacg catggtccgt gttgttagct ttgatcatgg caagaactgg
     4981 agaaagtttt tctcttgata gcggataatc tgggcctacg ccttccaatt cagcaatcag
     5041 cattttcgtt ccttttggta cactgattcc tgccaatttt gcgatttcca tcgcagaatg
     5101 tccaacgatt gatggattga ccgcatattt tccttcgttc atcactgcat cttctaattt
     5161 ttgcaattca tctggtttta cgatataaac ttggtgtgct tggaattctg cttttactgc
     5221 tgcatagatt tctttatcca cgatcacagc ttgttcagaa gcacaaatca ttccattatc
     5281 aaatgttttc gaaacgatca aatcgttcac tgcacgttta atttttgctg ttttttcaat
     5341 ataagctggt acgttacctg gacctacacc taaagctggt tttcctgtag agtaagcaga
     5401 cttgaccatt ccagctccac ctgtcgctaa gacgatcgcg atacctggat ggttcattag
     5461 catagaagtc gcttcgatcg aaggatgttc gatccattgg atacaatttt caggagctcc
     5521 ggcagcgata gcagcatcac gtaccactcg tgctgcttct gcagaagatt tttgggcact
     5581 tggatggaaa gcaaaaacga ttggatttct tgttttcaac gcgatcattg atttgaaaat
     5641 cgttgtggaa gtcggattcg ttgttggagt tactccgcaa accacaccga ctggttcagc
     5701 aatttcaatc aacccttttt ggacatcttc attgatcaca ccaactgttt tatcatgttt
     5761 gatattatgc caaatatatt cggacgcata catatttttg attgctttat cttcgtaaac
     5821 accgcggcct gtttcctcta cagccatctt cgctaatggc atatgttgat ccaaagctgc
     5881 cattgccatt tcatgcacga tatggtctac tttttcttga tcaaagcttt ccatttcttt
     5941 caatgctgca tttgcttttt ctgccaaatc atcaatcatt gcttgtacgt caattgtctt
     6001 cgttttttct ttttctatta ctttagccat cattatcctc ctaatgggtc atggttattt
     6061 gttaaataat tcacatgtat atcatacaat atttattttt ctatgtaaac acttttttgt
     6121 gatttttttc acagttattt ttaaatataa aattgcttta tttacaggtg tttgtataga
     6181 aaaatatata aagttattta ttattattta tatgacacac ccgattattc aatattgagc
     6241 aatatatccc atgctcttat ctctttcttt tttgaataaa aaatgaggta ggacatcttt
     6301 ctgccctacc tcgtctttaa ataagctttg atacttattt ttcttccttc tcttctgtct
     6361 cttccagcgt ttctgttgtt tcagttttta cttcaactgt ctcagctggt tcttctgttg
     6421 tttcaacagt agtcgttgca tcatttgtta cgacagtccc tggtttgacc gttttgattg
     6481 ctgcacgatc gtactcaagg aagatccctt cgcagtctaa aacaactgtc cgtttgtcat
     6541 tgtcgatttc tgaaacaact ccatgtaatc cgccgatcgt tacgactgaa tcacctggtt
     6601 tcatgctatc taacaagttt tgacgttctt gctgttgttt cttttgcgaa cgtgacataa
     6661 aaaaccacat tgctaatagc gcgataaaca tcacaagcat tggtaatgtt cccatttttt
     6721 tcacctcact aattctgtat ctacaaaaaa cactttagca gaaaaatgct gataacacta
     6781 gttttttact taatttttaa aagattttcc tttagaagct ttttgcgttt tctttattga
     6841 acccgtattc ttcaaaaaag gcttctctaa attcaagcaa attatcatcc atgattgctt
     6901 gacgtacttg tttcattaga ttcaacaaga agtataaatt gtggtaagaa gtcaaacgaa
     6961 tcccaaatgt ttcatcacat ttgatcaaat ggcggatata ggcacgtgta tagtttttac
     7021 atgtataaca atcacatttc tcatcaattg gacggaaatc atgggcatat ttcgcatttt
     7081 tcactactag acggccttga gaagtcatac atgtgccatt tcgagcgatt cgagtaggca
     7141 agacacagtc aaacatgtct actccacgga taacaccatc gatcaatgaa tcagcagcac
     7201 cgacacccat caagtaacgt ggtttgtttt cggggatcaa cggtgtagtg aattctaata
     7261 cacgattcat ttctgattta ggttccccga cagataaccc gccgattgag taaccaggga
     7321 aatccatccc gactagatct ttcgcgcttt gacgacgaag atcttcaaac cctgctcctt
     7381 ggatgattcc aaataatcct tgacggtctg gattagcatg agctttcaat cctcgttctg
     7441 cccatcgaga cgtacgttct actgattttt tcacgtagtc gtaactttca tcgaatggtg
     7501 gacattcgtc aaaactcatc atgatatcag agcccaattt attttggatg ttgattgctt
     7561 tttctggtga taaaaacata cgcgaaccgt tcaagtgatt tttgaaatgc acgccttctt
     7621 cttcgatatt ccgcatatca cttaatgaaa aaacttgaaa gccgcctgaa tctgttaaaa
     7681 tcggttgatc ccagttcata aatttatgca gtccgccagc ttcttctact aagtcttcac
     7741 cagggcgtag ccataaatga tatgtattac ttaaaataac gccagcaccc atttctttca
     7801 actcttctgg ggacattgtt tttactgtag ctaatgttcc tactggcatg aacattggtg
     7861 tggggaaagt tccgtgtggc gtgatcaatt cccctaaacg cgcgccagta tgtttttctt
     7921 tcttgattaa acgatagcga atggcaggtt gcgtcattca tttccctact ttctatataa
     7981 aatatcttga caactcccac catcataatc gtttcaagtg ctttttgcaa gttccctcgg
     8041 cttccttcta ctttatttaa tattcttttt tttatccttt ttttatgaat aacaaaaaaa
     8101 agaaaaacat tatgacagaa atattttttg atacatggta tattggtgtt attaaagaaa
     8161 aggagggaaa aatcatgcaa tcttctgcag aattaaaacg ccaagcaaaa gagagtctaa
     8221 aaggacgttg gggacaagct gttttactaa atctaatccc tacattgatt ggtattgcta
     8281 ctattcttct tattatgatt cctattatct tgtttgccac caccatatat gatggatcag
     8341 cgagcgtctc agaagcgaca acttcaggag gcggtagttc aggaggcggt ggcggaatta
     8401 tttccagcat cattagcgct ttgttcatga gcggtatttc ttggacttat cttgatattt
     8461 tacgtggaca aaaagatcag atcgaaccat tcaaagatgc atttcgtgga ttcaaaggtt
     8521 tgtttatcgg cggagtgatc agcttagctg tgctgatctc gatctttaca acattatggg
     8581 gtttgctatt agttattcct ggaatcatta aaagctattc ttattctcaa agctatttta
     8641 tttactacga tatcgtccaa gaaacaggac aaacacctaa agtactggat acgatcactg
     8701 caagtcgaaa attgatggat ggttataaag gacaactttt ctggttagat ctttcattta
     8761 tcggctggca tatcttagca ttagcaactg ctggtattgg ttatctatgg ctcagcccat
     8821 acatttctgc aacaaaagca gcattttatg aacaattgcc aaaagatatt taaaagagag
     8881 tgtgacttgt gaccgagggg gtggcaggag aacaccgttt atcgagaggc agtaaaagaa
     8941 acattcagcc cattagcaaa aaggaggtgt gacaacattc atgtcacacc tcctttttgt
     9001 atagttccag taaaaagaaa atcaacttag aaacagttaa tcattcctct tcttctgctt
     9061 ccggtaatcg atggaacttg atcagttcat tcaccgcgat tggtacgata gataaaatcc
     9121 ctaccaagaa ccagtgattc aaactgatgt gtactaggcc gaatagttct tgcgtaaatg
     9181 gcagcaaagc aacagccgtt gtaatagcta atgccagtaa cgccatttct accaaagctt
     9241 tattggtcgc taaattgact ttgaagatcg attggctgct gcgtacattg aacacatgca
     9301 gaatcgatga ataagctaag atcaagaatg caacagtttg accgacatgc tgacttgcgt
     9361 ctacccatgg gctgatagta tcgacataac tacctaaata ataaccaaac aaggttacga
     9421 tggtgaagac ccctgcattt atggcaattt tttgcaataa acctcgagca aagatccctt
     9481 catttttcgg aatgggtggc tgatccatga tgccgtgttc cgctggctct cttcctaaag
     9541 caaatcctgg aagtccatca gcaactacat tgacaaacag tagttgcaca gctgtaaatg
     9601 gtgctcccca tcctaacagc atcgcaatca aaacaataaa gatctccgaa atattacagc
     9661 ttagcaaaaa gttgatagct ttacgaatat tctgataaac cgaacgtcct tgactgacag
     9721 cgtctacgat cgtcgcaaag ttgtcatccg tcaaaatcat atctgcggca ctttgcgcca
     9781 catctgttcc tgtgatcccc atggcacacc caacgttact tgctttcaac gctggtgcat
     9841 cattcacacc atccccagtc atcgctacga ctgcacccgt acgttgccaa gatttgacga
     9901 tccggatctt gtcttctggc gttacccgag catagacaga gagagctttg acacgcgcat
     9961 ctaattcttc gtctgataat tgatgcagtt ctgatcctga aagtgcttct gacggatcat
    10021 tcaagatacc taattctttc gctatggcac tggcagtcac cacatgatct ccagtgatca
    10081 tgactgtttt gatgccagcc tttttcgctc gcttgattgc tcctttactt tctggacgtg
    10141 gtggatcgat cattccgatc agaccaatca aacgtaagtc tttttccagt gcttcagaag
    10201 aaatatcttg cggttcttcg tcataagtag cataaccaac agcaatcact ctcagtgccc
    10261 gtttaccaaa gcgatcatta acgatcgccg cttgttctac atcgcctgaa ctaaagcgcg
    10321 gagcaagaac atcgaatgct cctttggtga ttgagatgta tttttcgccc atttggtgaa
    10381 ctgttgtcat catttttcgt tcagaatcaa aaggaagctc gtttactcgt ggatattttt
    10441 cttctaattc tgcctttgta tgataattct cttcaacagc ccttacgatg gctacttctg
    10501 ttggattacc tttgataaca aggtcgtcat cttcttgctc gaccgtcaca tctgtacata
    10561 gtgcagccat tttcaatatt tccatcgcgc tgttcgtcat tgaatcttcg atattcgtta
    10621 cttcatcttc tttgctccat acccggcgga cgcgcatttc attttgtgtc agcgttcctg
    10681 ttttgtctga gcagataaca ttagcggttc ctagcgtttc aactgctggc aactgtcgga
    10741 tgatcgcatg tttcctcgcc atcttctgca caccgtatgc aagtgtcaac gtaacaataa
    10801 cagtcaaggt ttccggtaca gctgcaacag ccaaggaaat cgaagtcatg aacatctcaa
    10861 ggagtggttc tccttgcagt tcaccaatca caaatactaa agccgcagcc gctaaagcga
    10921 tcatactgat tcgtttgcct agctgattca atcggacctg taaaggtgtt ttttgcatag
    10981 tattttcgtt caaaagatcc gcgatcttac ccatttctgt agccatgcca atggcagtga
    11041 cgatcgcttt cccacgacct gcagcaaccg tacatccttt aaataccata ttaagttggt
    11101 cacctaatga ctcatcttct tcagcaataa atgctgcatc tttttcaact gcttcacttt
    11161 ctccagtcaa cgccgattct tctacacgta attgtgatgc ttcgatcaaa cgggcatcgg
    11221 cagaaatagc atcaccggac tctaaaacaa gaatatcacc cactaccagt tgctctgact
    11281 caacagtggt cactttgccg tctcgtatga cagttgccgt ttgtttattc atatcttgca
    11341 aggattccag tgctttttct gcgtttcctt cttggacaat cgccaatacc gagttgatca
    11401 caacgatagc gatgatcagc agtccttcaa ataaatcccc atgttctgta gcaaatgcgg
    11461 tataaaagga aatcaccgct gctacaagaa ggatgatcgt tgtaaaatcg gacaagcttc
    11521 gaaagaattt tttgatcatc gattctttcg gcgcctcatc gaatttattc aatccctgct
    11581 tttttcgccg ttcatccact tcttgtgaag acaacccagt atctggatct gcttctgtta
    11641 ccttctttac ttcttctatt gtctgcttgt attcttccat cctattcctc ctttaaaatc
    11701 ttcgtacaaa aaaaggtact tattttacta taacatcctt tttttattta cttcaaataa
    11761 agggcttatc gattatttgt taagaaagcg taaaatttaa agtgcttata tttgcaaacg
    11821 ctaccaaagg atgctattat atagatgtaa ggaaggagga taccatcaaa tagacagaag
    11881 aatttctcga tagaacagtg taagcaaaca aagttgttag aagtacaggt ttcacccaga
    11941 catgcgtgta gagtacaaac ctataaatag tgaatgcgta tggatgtttg gatcacgaag
    12001 tatccgaaac aagaagtaat gcgatacgta acaattgaat aacgtaaacg tctgaaaatt
    12061 gaataggaac aagagatgtt tcaaatgttg aagatgtaac gaaaacaaaa tttttgttta
    12121 acatgaagaa attcaacacc gtgctttgca tgtatgatgg aaagttacaa tcatttctgt
    12181 ttatttgaat ccttccgagg tacaagaaag ccgagcttat cgagagctcg gctttttctt
    12241 tgtcataaaa tctttgtaaa gcttttttgt tattcaaaac tagcggcttt tcataacctt
    12301 atttcacgaa catcgcatct ccaaaactaa agaaacgata gcgctcttca atcgcatgct
    12361 ggtaagcaga taaagtcaat tctcttcctg caaaggcact aactaacatg actaaagtcg
    12421 attttggcaa atggaaatta gttgagaaag cttctaccac tttgaattca tagccaggcg
    12481 taatgaaaat atcagtccat ccgctgtctg ctttgatttc tccattaaat ttcgttccaa
    12541 tcgtttctaa tgttcgaatt gaagttgttc cgacagccac gattttcccg ccatttttac
    12601 gtacttggtt caatcgctca gccgcttctt ccgtcaaacg gtagaattca ctatgcatat
    12661 ggtgttcttc aattttatct acgctgacag gtcggaatgt acctaaccct acatgtagtg
    12721 tcagatagac taattcaaca ccctttgctt cgatctcatc aagtaattct ttcgtaaaat
    12781 gcaatccagc tgttggcgct gcagcagaac cattttcttt cgcatagaca gtttgatagc
    12841 gatctgggtc ttccagacgt tctttgatat atggaggcaa tggcatttct cccaatgatt
    12901 caagaatctc tagaaaaatt ccttcatact caaagtcaat gatccgtccg ccgtgttcca
    12961 attcttcttt cactactgct tgcaatctgc catcaccaaa agagattctt gtcccaactt
    13021 tggcacgttt cgcaggtttg atcaatgtct cccacgtatc tccttgtgta ttcgttagaa
    13081 gaaggacttc taaatgcgca ccagtatctg gtttttctcc atataaacga gcaggcagaa
    13141 ctcgtgtatt attcataact agcgcatcac caggctgtaa ctcttcaatg atttcatgga
    13201 aatgtttatc ttccatctct ccagttttcc gatccaaaac aagtaaacga gaatgatcgc
    13261 ggtcttttaa tggtgtttgt gcgattaatt cttccggcaa gtcaaaatca aaatcttctg
    13321 ttgttaacat atcgtcactc actttctatc atgtgcttca tcattctatc atgtttttcg
    13381 ttctgttggc aaaatcagca ctttttcact tctatttttt gttttcctaa ataaaaaaac
    13441 taccctattt gtattttttc attgttttag gcatactagt aatagatagt tcttcattga
    13501 aaatagaaaa agaggtggca ccatgattga atttcaaaat gtgtcaaaaa tttataaagg
    13561 tggaaaagtc gctgtagagg acgtcaatct ttcatttgaa aagggtgagt tcatttgttt
    13621 tatcggtacg agcggaagcg gaaaaacgac gacgatgcgg atgatcaacc gaatgactga
    13681 tccaacacaa gggaaaattt tgattgatgg aaaatccatc caagaaattg atccagtgga
    13741 attgcgacgt cagatcggct atgttatcca aagtattgga ttgatgcctc acatgacgat
    13801 ccgtgaaaat atcactcttg tccaaaaatt gttgaaagtc agtcaagaag aacggaataa
    13861 aacagctgaa aaaatgattg atcttgtgga attgccccgt gagatgttag atcgctaccc
    13921 acatgaatta tctggtggtc agcagcagcg tattggagtc gttcgtgccc ttgcagcgaa
    13981 tcaagatatc atcttgatgg acgagccatt cggtgcgctt gatccaatca ctcgtgattc
    14041 cttgcaagac cttgtcaaag atttacaaga gcgtttggga aaaacgatcg tatttgtaac
    14101 ccatgacatg gatgaagc
//
LOCUS       DAZABD010000067        14019 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000067 DAZABD010000000
VERSION     DAZABD010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14019
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.067"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(325..2322)
                     /gene="tkt"
                     /locus_tag="ACHALG_002206"
     CDS             complement(325..2322)
                     /gene="tkt"
                     /locus_tag="ACHALG_002206"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFN2587575.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPIGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2424..2675)
                     /locus_tag="ACHALG_002207"
     CDS             complement(2424..2675)
                     /locus_tag="ACHALG_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HFN2587576.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2822..3445
                     /gene="lexA"
                     /locus_tag="ACHALG_002208"
     CDS             2822..3445
                     /gene="lexA"
                     /locus_tag="ACHALG_002208"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFN2587577.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3624..3842
                     /locus_tag="ACHALG_002209"
     CDS             3624..3842
                     /locus_tag="ACHALG_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587578.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(4130..4642)
                     /locus_tag="ACHALG_002210"
     CDS             complement(4130..4642)
                     /locus_tag="ACHALG_002210"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFN2587579.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4656..6953)
                     /gene="recJ"
                     /locus_tag="ACHALG_002211"
     CDS             complement(4656..6953)
                     /gene="recJ"
                     /locus_tag="ACHALG_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFN2587580.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLMHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPVE
                     FLDLVAIGTIADLVSLTDENRILVSLGINAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSN
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     IMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(7095..7538)
                     /locus_tag="ACHALG_002212"
     CDS             complement(7095..7538)
                     /locus_tag="ACHALG_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HFN2587581.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7539..8327)
                     /locus_tag="ACHALG_002213"
     CDS             complement(7539..8327)
                     /locus_tag="ACHALG_002213"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFN2587582.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETDFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8324..9265)
                     /gene="rnz"
                     /locus_tag="ACHALG_002214"
     CDS             complement(8324..9265)
                     /gene="rnz"
                     /locus_tag="ACHALG_002214"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HFN2587583.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9442..10029)
                     /locus_tag="ACHALG_002215"
     CDS             complement(9442..10029)
                     /locus_tag="ACHALG_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HFN2587584.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10250..11563)
                     /gene="obgE"
                     /locus_tag="ACHALG_002216"
     CDS             complement(10250..11563)
                     /gene="obgE"
                     /locus_tag="ACHALG_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFN2587585.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11851..12861
                     /gene="gap"
                     /locus_tag="ACHALG_002217"
     CDS             11851..12861
                     /gene="gap"
                     /locus_tag="ACHALG_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFN2587586.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12946..13881)
                     /locus_tag="ACHALG_002218"
     CDS             complement(12946..13881)
                     /locus_tag="ACHALG_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFN2587587.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         3879 a         3135 c         2464 g         4541 t
ORIGIN      
        1 gagcaatcgc ttttttattg atcaccattt ttcttttagg tctatggtgt cgggcactgc
       61 gggtcttcag ctcttgcatt caaattcctc ctcaaaataa aaaaacttaa taaaaagttc
      121 tcttcttaaa acaaacttaa ttaattaaat tatactttta attctcaaga aacacaaact
      181 aatccgaaga attctttgtt ttgtcattta aatgtaacat agccgaaaac gaaaagtaat
      241 attgatttat tcttctaaca cataaagaac agctgtctcc aacttgcaga cagctgttct
      301 tatcagtcaa acaattgctt ggatctatga tagttgattg tacgtattca cgacattttc
      361 tactgtaaat ccaaattctt ctaaaatttt agtgccaggg gcagatgcac cgaaatgatc
      421 gattgtaatc gttgcgccct ctgttcctac gtagcgttcc caaccaaatg atgcagcagc
      481 ttcgatagcc acacgtttct tcactgattt aggaagtaca ctttctttat attcagaaga
      541 ttgtttttca aagaggtcaa agcttggcat agaaacaaca gaaacatctt tgcctttttc
      601 agccaattct ttttgagctt tcacagccaa atcaacttca gaacctgttg caatcaaaat
      661 accttctggt gtttctcctt gtgatggaga aagaacatac gcgccttttt tcaccatttc
      721 atctgctact tcttttgtag aaggcaatac tggaaggttt tgacgactca atacaaggat
      781 agtaggcgca tctgttgtct ccatcgctac tttccaagca gcacgtgttt catttccgtc
      841 tgccggacga atcacttgaa caccaggcat gcagcgaaca gatgctagtt gttcaatcgg
      901 ttcgtgcgtt ggtccgtctt ctccgacagc aaccgagtca tgtgttaaga cgtaaacaac
      961 aggagtatgt tggattgctg ctaaacgaac tgctggtctt agataatcga caaagacaaa
     1021 gaatgttcca ccgtagatac gagttccgcc atgaagttgg ataccattca tagccgaagc
     1081 cattgcaaat tcgcggacac cgaaccagat gttgcgtcct tcataatgtt caggagtgaa
     1141 atctttatct gcagccacca ttgtattatt ggaacctgac aagtccgctg aaccacccca
     1201 gaagcttggt atagctttcg ataattcttg gatgacttct ttacttgaaa cacggcttgc
     1261 ttgactgctt ccaacttcat aagttggcaa ttctgcatcc cagttttctg gtagtttgcc
     1321 atcaaaagca tcttcaaatt gttgtgctaa ttcaggataa gcttttttat aattggcaaa
     1381 catttcattc catgcatctt cagctttttg tccttcttct atcatcgttt gatggaagcg
     1441 ggcagctact tcttctggaa cagtgaaatc tggatactcc caaccataaa cttctttagc
     1501 catcttgatg ccatcttcac caataggagc accatgaaca gcagatgttc cttctttagg
     1561 agcaccatag ccgatgactg tttttacttc gatcaatgtt ggtttgtctg tttcagcttt
     1621 tgcttcttca atcgctttag agattgcttc taggtcattt ccgtctttca ctaagatatg
     1681 ttgccagcca tatgcttcat agcgagcacc aacgttttct gtgaaagctt ttgaagttgg
     1741 accgtctagt gaaatatcgt ttgaatcgta taaaacgatc aatttcccta gtttcatatg
     1801 acctgccatc gaacttgctt cttgagatac cccttccatc aagtctccgt caccgcatat
     1861 tgcatacgta tagtgatcca tcacattaaa gttttctttg ttatacgttg ctgcaaggtg
     1921 cgcttctgcc attgccatac cgacagccat tgcaatccct tgtcctaatg gccctgtagt
     1981 ggcttctaca ccatctgtgt gatgtacttc aggatgtcct ggtgttttag aatcccattg
     2041 acggaattgt ttcaaatcat cgattgtcac ttgatagcca gctaaatgaa gcaagctata
     2101 caacattgct gacccatgtc ctgccgaaag aacgaaacgg tcgcggtcag cccagttttt
     2161 tgatgtcgtt ggatttactt tcaggtgttt tgtccataac gcatatgcca taggtgctgc
     2221 gcccatcggc aatccaggat gtcctgagtt tgctttttgg actgcttcaa tacttaatgt
     2281 acgaattgtg ttaacgccaa gttggtcaat tttgtcaaac aaaataaccc ctcctattaa
     2341 tttaggtttt ttttaccttt atttcttaca ctttcattct ataagatgtc acacgaaaaa
     2401 gcaatctttt tcagtatttt ttactaaaag ttgtcatttc ggttgtgtaa gccttttttc
     2461 ttttggattt cttttaattt ttccggggtc acgtcattgc cctcaggatc gacgactttc
     2521 attccttcga tatgatgacg cataccggaa cgaaaagctt ccaaatactc tttgcgtaag
     2581 ttgatttgtt cttgttcttc ggcttttgag agaccatttt ccttcttttt acgtgcaagt
     2641 tcattgatcc tctctatttt ttcaggtgac agcatgctca gccctccttt tactctcctt
     2701 catattatat aaaaactgaa aaaaaaacaa ctagataaac taaataaaat acgaacattc
     2761 gtttgattta gcgcttttta tatggtacta tttatttatc aagtgaaaga aggtgtacct
     2821 tgtggcccga caaactgaat caagacaaat cgaagtctta cgatatatcc atgaacaagt
     2881 aacccaaaaa ggatacccgc cgactgtccg tgaaatcggg gaagctgttc agctttcttc
     2941 tacttctact gttcacggtc atcttgcacg gcttgaaaaa aaagggttta tccaaagaga
     3001 tccgacgaaa cctcgtgcaa tcgagttgac aaaacaaggt ttagaaaaga ttgggattcg
     3061 tccaacaact attccagtac ttggcgtcgt aacagctgga gaacccattt tagcagtaga
     3121 ggatgcttca gacttctttc caatccctcc taatctttct tccgaagaag gcagtctctt
     3181 tatgctgaca attcgtggag atagtatgat caatgctggg atcttggatg gtgacaacgt
     3241 gatcgttcgg aagcaagaaa ctgctcaaaa tggcgatatt gtcattgcaa tgactgccga
     3301 ggacgaagcg acttgcaaac gattctataa agaaaaaaac tttttccgtc tgcagcctga
     3361 aaacgacttg ctcgaaccaa tcatcttaga tcaagtatct atattaggac gagtagtagg
     3421 actttatcgt agtcatatct attaattgct actaataaaa gaaaactttt taaaaagagt
     3481 ttgagacgat ggtctcaaac tcttttttcg cccgggttct tcttctttca aatttttatc
     3541 acttccctct ttacaaaccg aacaaatatt cgtatactaa atatacgaac aattgttcgc
     3601 ataaattaac gaggtgagaa ttcatgaaat caatgagacg tcttggctta gtattaactg
     3661 ttttgttttt aggtattatt attggtaatt tcggcatacg tccagcttta cttgtgtttg
     3721 ttccgttatt tactttgtgg ttcatgttgt gggatgaaaa gaagtaccgt caatctgagc
     3781 ggcgcagaat tcatgaagat catttctatc atgagcccta ctacagatcc aggcaatcat
     3841 aataaggatt gactgctaat tattcaaaag acaagaagtt aaacttttac gggggtgtga
     3901 caaaaatcgg tcacatcctt ttttcgaata aacggtgttc taaagccagt ttttcggtat
     3961 taagtcaaat tgcggaaaaa gtgatttttt aaacttctac tggtctttgc ttgtttccat
     4021 ttataaaaaa atagcgaccc tcaaaagtca gatagagaat ctgacttttg agggtcgctt
     4081 taaaatcgct tctttaagaa taacatcaag aatagcttct tttttctgct taatattcca
     4141 ttaatgtaat gatgtcatat cctttgattt tgtcgcgtcc atggagatcc atcaattcga
     4201 tcaaaaatgc gcagcctacg actacaccgc ctaattgttc gatcagttcg atcgtcgctt
     4261 tgattgttcc tccagtagct agcaaatcgt cacaaatcag cacacgttga cctggtttaa
     4321 tggcatcctt atgcaatgtc aatgtatctg ttccatattc taatccataa gtcacttcga
     4381 tggtttctcg aggtaacttc ccttttttgc ggactggggc aaaaccgact cctaactcat
     4441 aagcaacagg acatcctacg atgaatccac gggcttctgg cccaactacc atatcaattc
     4501 gtttttcttt tgcataattt acgatttgct tcgtagcttc ccgataagcg tcaccatctg
     4561 ccatcagtgg ggaaatatct cgaaacacaa ttccttttga cggatagtcg ggaatactgg
     4621 caatataatc ttttaaatcc actctttgtt tcctcctagg ttgttaacca tttctttatg
     4681 gtaggaatat cgctcaaaag caaaaattcc tcaactttga ttttattgag ccgccgctgg
     4741 tataatctgc tctcagtcaa aggatgactt tctgggctag cgactttttg taaaacaccg
     4801 ttctctattg taacaaattt caattcaaaa aacacctgta tcataaaaat taataatttt
     4861 tcagggatac gcaaatattg agctattgct tttattttat gacgtatatc aattcgttct
     4921 tgagaatgga tcagtttaaa caattttgcg tactgttctc ttgtgcctac cccattcaaa
     4981 taggcctcat caggcgaaat ccccatcaga taaacacgat tgaatgaata agaccgaaaa
     5041 acttctttca gatccgctaa atcatctgga caatccataa taactagaga gttcggtttt
     5101 cttttttcaa ttattgcctt tacagaatct agtccttcga aaacaatcgc agcttcctgg
     5161 ataatctcag gcacgaattt caacgtagaa gaatcaaatg ctaaataaag gctaccttcc
     5221 gacgttcggc tctgttcacg gaaacgtttt gcccgccaat caaatacttg cagaccaccc
     5281 acagaaaaat cagataccat cagctgcggt ttcttacgcc cgttccattc attgatcgaa
     5341 agctttccta ccacatctac ctgattattc agcagttctt cttcttgagt accaaaacca
     5401 aaagcgaccg catcaagctg actcgatgca ttggaaagga caaatttcag atgctgttgg
     5461 tttgctccga ttcttttgat attttctgct tggacttgtc gaaataaaaa ctgaggcaaa
     5521 ggattatccg taccaaatgg cgccaatagt ttcagctcat caattcgctc tactgttact
     5581 tcatttggca gcaatacttc atcaattctc aactcaggcc ctctcatcaa gtcaatttga
     5641 tgatcgacta tatattggtt catcttctcc tgtagtatag tgacattttc acttggcatc
     5701 gttaaaccga ctgctgcatg atgtcctccg aagtaagtaa ataaatctcg catctggtca
     5761 agcatttcaa aaagattcaa ggcttcaata ctacgtccag atcctttagc tgatccatct
     5821 tcttttaaag taaggacaag cgttggttta cccgtttcat tcattatttt cccagcaaca
     5881 atgcccaaaa cgccttcatg ccatcctcgg tttaccaata gatggatatg attctcctta
     5941 ttgaccatcg ctaatgcttc ttgtgttatc tgctctacga ttgcttttct ttcttcattg
     6001 atctcattca atttttttgc ttgaacggat gcttcctctt catcaaacgt cgcaagtaac
     6061 gagacggcag gacgtggatc gcccatccga ccgattgcat ttagtctggg agcaatcgaa
     6121 aaaccgatcg ttgtttcgtc tgcgtccctc atttttacgc cgctttcttc aaatagcgcc
     6181 tgaagtccta tccgttctga atgatgaatg gcattgattc ctaatgaaac aaggattcga
     6241 ttctcatcag tcaatgaaac taaatcggcg atcgttccta tcgctactaa atccaaaaat
     6301 tctacaggcg gttcttccaa taatgcagaa gctactttga atgctactcc gactcctgcc
     6361 aaatctggaa atggatagtg ccccgctgga tgacgagggt gaacaatcgc ataagcgtct
     6421 ggtaaattct caggtagttc gtgatgatct gtaatgatca cgtctacacc agcttgctga
     6481 gcgtatgcta ccgcatcatt tcctgctacc ccgttatcaa cagtgatgat caactggatt
     6541 ccttcttcga tcttttcttg gtatacagat tggtttggtc catacccatg ctcaaatcga
     6601 tttggcagga atacttctac ttctgcacca agtaattcca gtgtttcttt cattaccgtt
     6661 gcactggtga tcccatctgc gtcataatca ccgtaaacga ggattttttc tccactgatt
     6721 acagcttctt ggattcggct gattgcttta tccatatcgt gcatcagata cggatcatgt
     6781 aattcttgtt cctttggatg taaaaatcgg ttgatatcct tttcctcatg gtaacctctt
     6841 tgccacagta acttaccgac aaatggagat aatttcagtt cacggatcat ttggagaaag
     6901 gattcagaag gctctgtctc gcttaacttc caatcaaaat ttgcttgtct cactttctca
     6961 ctcctaactt agttattata gcaaaggaaa tcagcaaaaa aaagcagaaa gaaagaataa
     7021 aaacagatca gatccgctag acagatgttc agcaggacct gatcttttgt acgaccattt
     7081 atgaaatatc cctattagtc aaaataattg aagctttgtt tgggcgattg agttttttgt
     7141 tcttccggcg ctttcaattg actgatttgt gcggttttcg cttgtaactc cgcctgataa
     7201 gcagcttcca actccgcacg ttcgtttcta aactcgcgtt gctgttcttc ttttgcttct
     7261 tcaattttct tttgaagttc tgtttgatat tctgacattt cctgagtcag ctgtttgatt
     7321 tgcttttttt gctgccatag tgttgccgaa gcagtcaaaa atacaatcag tgcaccaatc
     7381 agcgcagaac cgatgatcac taagatcaat ggcgcagaaa aggaagtgaa gccaaaactt
     7441 acaggaacat ttttattgtt caatacagca aaaagaacaa taatcaaaac taaaacaaat
     7501 ccaagaatca cattgctttg ctttttcaat tccttcacct actttttgtt aaaaattccg
     7561 ctggctaaat aatcaccaat cgttggaaac agcgtataaa attttgatgc aacttctaaa
     7621 ataaacggtt gattgatttc ccgttttgga tgtctcatat tgcggacgat catttttgcc
     7681 aacttggcag gttctaaaac gatttgtccg actttttcca aatagcttcc gcttggatca
     7741 gccttatcga agaaatctgt ctcgattgga cctggattca ctgtcgtaac agctacgcca
     7801 agtggtttca attctaatcg caaagcgttg gagaagccaa ggactgcaaa tttcgtggca
     7861 gaataaaccg ttgattttgc agttgccatt tttccagcca tagacgcgat attaatgata
     7921 tgaccttgtc ttctttctgc catatcgatc gcaaattttt gtgtcaaaac catcattcct
     7981 aagatgttga cttcaaacat atcgtatgct tttccaaggt cgaatgtaag gaagtcttcg
     8041 aaaatcccaa aacccgcatt gtttaccaga atatcgacgc gcccgacttc ttccatcacc
     8101 ttttcatata attcgtccac gctttcagga ttgctgacgt ctaccttgaa agcataagcc
     8161 tctttgccgc tcaagcgtct gcactcgtct ctgacacctt ctacaaatgc cattcttcgt
     8221 gcgcaactta taacgattgc tccttgcttt gctgcttcat aacagatttg tgcgcccaga
     8281 ccgcctgaac tacctgttac taaaacaact ttatcttcta aattcatgcc tcttcctcct
     8341 cgcgaaatgg aatctcaatg ctatcaaaat ctttcatgat cgtcgtgttt tcaaacactt
     8401 cttgtgcttc tttttcaagc tctaaagctg cttttcctaa atatctcgca ctgatatgag
     8461 taagtagcag ccgttttgcc tgcgcttctt ttgcaacttc agcagcttga tgagtcgtgg
     8521 aatggaaata gttgtgcgcc attcttgctt catctttatt aaatgtgctt tcatgtacca
     8581 gaatatcgct attttccgca agcacaacgg aattatgtgt ttttctcgta tcgcctaaaa
     8641 tagtcacgat tcgtcctttt ttatctggac ctacgaaatc ttttccattg atcgtccgcc
     8701 catcttctag ttggatcgtt tcaccttgct tcagtttgcc gtacagtggt ccagctggaa
     8761 tacctaattc ttttagacga tccacctgca attctccttt atgatcatgt tcaaccacac
     8821 gataaccaaa actgtcgatc ccatgattta gtttttttgt ataaacagaa aattgctgat
     8881 cagtaaaaat cggggctgtt tcatttagct caatgaattt cagcggataa cttaaacgcg
     8941 tttgcgaaat acctaaagag acttttatgt attcctctat cccttttggc ccgtaaatct
     9001 ctaatggcgt atccccacct tgaaatgacc ggctgctgat gagcccggga agtccaaaaa
     9061 tatgatcgcc gtgtaaatgt gtaataaaaa ttttaccgat ttttctcgga cgaatagtcg
     9121 ttcgcaaaat ctgcatttgc gtgccttcgc cacagtcgaa caaccaaact tcattgcgct
     9181 cgtctaaaag tttcaatgct atacttgtca catttctatg ttttgcagga acacctgcac
     9241 ctgttcctaa aaattgtaat tccataatct acctgacttt ctttaagttc ctctttaatt
     9301 ttagggtaaa aagaagggat aatcaaccgt aagtcgagaa tgggttcaaa ttaaaaataa
     9361 cgttctaaag cgatagcgtt ttctttgaaa attactataa aaacagcagc gaacccgttt
     9421 gagatcctct gctgtttttt attaatttga agatccagaa ctttcattgt cgttggaagt
     9481 ttgcggatgt ttgtccttcc aagtcttttg tgcagcttct ttcgcatgat tttcagcttc
     9541 tttcgcatca tgcacatctt tttcgacatc ttcataatcc aagcgggtaa tttctccgcc
     9601 taattccatc attaccaatt gattcagtgg gcgattgtcc tcttcatcag caatcaagat
     9661 cagtcctgta gctccttcac cgattttatt tcctacaaag tcgaacacgg tctgggcatc
     9721 ttttatttct ttggcatctt gtgtcgcacc gatcattcct ccggcaaacc atcctaataa
     9781 aatccctagc ggaccaccta atacaccaac caacattccg attaatccac ccgtagaaga
     9841 tttgttatta ccggtaaagt cgataaaatc ttctattttg aattgatgtg tcccattagg
     9901 atcatggctg acaactgcca tctgttctcc tttaatcgaa cgaacagcat gtaacttttt
     9961 gatttctgaa aatgcctgat atgcctggct ttctacttga aaatttaaaa tgattactcg
    10021 tttgctcatt aaaaacgccc ctttcatacc ttcttgttat ttattttacc tttaattgta
    10081 aaataagaaa agaaaagcac tctttttttc caataaaata gggctgtgac aaaagtcgta
    10141 tcacagctcc tttttccgaa taaacggcgt tcttgaagct gatcacttct gtcacaacct
    10201 cccttaaatc tgaatctata ttttttggta tttgttttta tttgcttgcc tattcgacaa
    10261 attcaaattc aaactcaccg atacgtacaa ggtcgccatc tttcgcaccg cgtgcccgaa
    10321 gagcttcatc cacacccata ccacgtaatt gacgagcaaa acgcatcact gtttcatcat
    10381 gatcgaaatt cgtcatttgg aataattttt cgatcttctc accagataag atccacgttg
    10441 catctgaatc acgatcgatt tggaactcag gaccttctgg attgaatcca tagtgtacag
    10501 tttcttcttc cagttcttct tcgtacaatg ggaattctgg cgttacttct aacaaatctg
    10561 ctgtagcatt caataacgcg tccaatcctt gtcgagtcac tccagaaatt ggaaatactg
    10621 ggatgtcatc agcaaattca tcttcttttt ccttgttcag ctgttctttg aatttcacta
    10681 gattttccgg tgcatccggc atatccattt tgtttgccac gatgatttgc ggacgctcta
    10741 gcaagcgtaa gttataagta gataattctt tgttgatggc aagatagtct tcataaggat
    10801 ctcgtccttc catcccactc atatcgatca catgtaaaat tactcgtgtt ctttcgatat
    10861 gacgaaggaa ttgtgtaccc agcccaacac cttgcgaagc tccttcaatc aagcctggca
    10921 agtctgctgc ggcaaaactt ctgccatcac ttgtagtaac cattcctaga ttagggacga
    10981 gcgtcgtgaa atgataagcg ccgatttttg gacgagcaga tgaaataacg gatagtaaag
    11041 tcgatttccc gacagatgga aaacctacaa gtccaacgtc tgccaatact ttcaattcta
    11101 gttcgatttt tcgctcttgt cctggttcgc cgttttcagc aatttccggt gcaggattcc
    11161 gaggagaggc aaaacggata ttccctcgtc caccgcgtcc accttttgcc acaactagtg
    11221 tttgaccatt ttcaattaaa tctcctaaaa gagcaccagt ctccgcatct cggacagtgg
    11281 ttccttgtgg tactttaaca taggtatctt ctgaaccacg accgtgcatc cctttactca
    11341 tcccattttc acctggctgc gctttgaaat ggcgattgaa ccgaaaatcc atcaatgtac
    11401 gtaacccttc atcgacgatc aagatgacat cgccgccacg accaccgtct ccgccagccg
    11461 gtccgccgtc aggaacgtat ttttcgcgac gaaatgcaac cattccgtct ccgcccttgc
    11521 cagctttcac atcgatcgtt acttgatcta aaaacatgga cataattaag tcctcctatt
    11581 attcatttat ttatttgact gcctgaattt ccacaataaa caaaagtctt acttatttta
    11641 tcggtgaaat ctgattttgt ctatcatttc agcaaaaaga ttgtttacct tcttcttttt
    11701 ttatcatttg gtaaggtttc caaccttttt tctgctacca agtataattt tttgacaaaa
    11761 tattctttgc gtagcagaat aacagtgtat taattgtatt aattgaggat gccgtttcac
    11821 agcaccccta ataaaggagg aacttttgat atgacagtaa aagtaggtat taacggattt
    11881 gggcgtattg gccgtctggc tttccgccgc atcaaagaag tctcagatga tattgaagta
    11941 gtagcaatca acgacttaac aagtccaact atgttagcac aattattgca atttgactct
    12001 actcatggta cttatcctgg cacagttact gcaacagaag actcaatcgt agtggatggc
    12061 gaagcaactc gtgtttatgc agaacccgac gcaagcaaaa tcccttgggc aaaagaaaac
    12121 ggtgtagata tcgtactaga atgtactggt ttttatacat ctgaagaaaa agcaaaagcg
    12181 catttagatg ctggtgtcaa acgagtagtt atttctgccc cagctggtgc tatgaaaacc
    12241 atcgtctata acgtcaatga tgatacatta gatgaaaacg acaaaatcat ctcagcaggt
    12301 tcatgtacaa caaactgttt agcacctatg gcttatttct taaataatga atttgggatc
    12361 gaagttggta caatgacaac agttcatgcc tacacttcca cacaaatgct cttagatgga
    12421 cctgtaaaag gcggtaattt gcgtgctgct cgttcagctg ctgacaatac tatcccacat
    12481 tctactggtg ctgcgaaagc aatcggctta gttatccctg agctacaagg aaaattacaa
    12541 ggacacgcac aacgtgttcc agtcgttgat ggttcattaa ctgaattggt ttcagtattg
    12601 aaaacaaaag taactgctga tcaagtaaac gaagcaatca aaaaacacac agttgacaat
    12661 ccttcattcg ggtatgatga ccgtcaaatc gtttctagcg atgtcattgg aacaactcaa
    12721 ggttcgatct ttgacccaac gcaaacagaa gtaacaactg ctggtgattt ccaacttgtt
    12781 aaaactgttg catggtatga caatgagtat ggatttactt gccaaatgat tcgcttacta
    12841 gaaaaattcg ctaacttgta atttgataga tcaatcgata aaagaagcct ttgactggat
    12901 ccagtcaaag gcttctttct tttcatcccc tacttttaaa ctcgttcaat gaacatattg
    12961 gcttaaaaat tgttggacta cttgctgata tttttcagga tctgtcttgt atgcttccgc
    13021 atgctcggct ccttttacaa catacttttc ttttggtccc ttcgttgcac gatagacctt
    13081 atctaacatt tcaaatggta caaatgtatc ttcgtctcca tgtataaaga gcattgggcg
    13141 ctggtttttc ttcaattgtt ccactgcatt tgcttcaccg aaaaagtagc cagcacggac
    13201 ttttgtcact agactcgtta cttgcataag cggaaatgct ggaagactaa acatatcgtc
    13261 tagctgataa gcaagctcat catgaacgga agaataaccg caatcttcaa tgattgcttt
    13321 cacattatct ggtagttttt ctccgctagt catcataact gttgctgctc ccatgctgac
    13381 accgtataga acgatttctt gcgattttcc attgttttct agcactttgt ttatccactg
    13441 tacatagtct ttacgctctg gccatccaaa tccaatgtaa tccccttcgc tttttccatg
    13501 tcctcttgca tcaggaacaa ggacattgta acccatatca tgaaacatct ttgcgtaatt
    13561 ggtcatcgtt tcagcgtttc ccatatagcc atgagccaca agagctgttt tttcactttt
    13621 cgtttcagca ggaagataaa tcgctgataa tttcagaccg tcttttgaag tgatggtcca
    13681 atcgcttcgg ttttcttttg aactaaacca tttatgtgca tccgtttctt tggatgtctg
    13741 tgttgtacca ggtgtatctt cgtctaaaaa gtctttctca gaaggcacaa cagcataatt
    13801 ataaaaataa ttccctgcaa acactaacgc aacaatcaga gctaaagcga cgcctaaaag
    13861 gattttcatc caaagtttca taaatcatcc atctcatttc tcttttttca tataaccttg
    13921 aattagtata atcaaagaat ctctactatg caatgttcat ttttataacc cgcgaattgt
    13981 aaaattaagt tagaaaaata aaaaggcatt tatggtaca
//
LOCUS       DAZABD010000068        13852 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000068 DAZABD010000000
VERSION     DAZABD010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13852
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.068"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..902)
                     /locus_tag="ACHALG_002219"
     CDS             complement(<1..902)
                     /locus_tag="ACHALG_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HFN2587588.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFSE"
     regulatory      complement(926..970)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            1185..2153
                     /locus_tag="ACHALG_002220"
     CDS             1185..2153
                     /locus_tag="ACHALG_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFN2587589.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            2266..2544
                     /locus_tag="ACHALG_002221"
     CDS             2266..2544
                     /locus_tag="ACHALG_002221"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN2587590.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            2662..2898
                     /gene="secG"
                     /locus_tag="ACHALG_002222"
     CDS             2662..2898
                     /gene="secG"
                     /locus_tag="ACHALG_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFN2587591.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            3001..3756
                     /locus_tag="ACHALG_002223"
     CDS             3001..3756
                     /locus_tag="ACHALG_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFN2587592.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            3759..6116
                     /gene="rnr"
                     /locus_tag="ACHALG_002224"
     CDS             3759..6116
                     /gene="rnr"
                     /locus_tag="ACHALG_002224"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFN2587593.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKFGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKDVKKKKKKK"
     gene            6138..6602
                     /gene="smpB"
                     /locus_tag="ACHALG_002225"
     CDS             6138..6602
                     /gene="smpB"
                     /locus_tag="ACHALG_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFN2587594.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            6904..7623
                     /gene="atpB"
                     /locus_tag="ACHALG_002226"
     CDS             6904..7623
                     /gene="atpB"
                     /locus_tag="ACHALG_002226"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFN2587595.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            7676..7891
                     /gene="atpE"
                     /locus_tag="ACHALG_002227"
     CDS             7676..7891
                     /gene="atpE"
                     /locus_tag="ACHALG_002227"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HFN2587596.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            7982..8506
                     /gene="atpF"
                     /locus_tag="ACHALG_002228"
     CDS             7982..8506
                     /gene="atpF"
                     /locus_tag="ACHALG_002228"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFN2587597.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            8493..9035
                     /locus_tag="ACHALG_002229"
     CDS             8493..9035
                     /locus_tag="ACHALG_002229"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFN2587598.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            9058..10614
                     /gene="atpA"
                     /locus_tag="ACHALG_002230"
     CDS             9058..10614
                     /gene="atpA"
                     /locus_tag="ACHALG_002230"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFN2587599.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            10628..11530
                     /locus_tag="ACHALG_002231"
     CDS             10628..11530
                     /locus_tag="ACHALG_002231"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFN2587600.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            11553..12959
                     /gene="atpD"
                     /locus_tag="ACHALG_002232"
     CDS             11553..12959
                     /gene="atpD"
                     /locus_tag="ACHALG_002232"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFN2587601.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            12973..13395
                     /locus_tag="ACHALG_002233"
     CDS             12973..13395
                     /locus_tag="ACHALG_002233"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFN2587602.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         4575 a         2474 c         3013 g         3790 t
ORIGIN      
        1 ctttcggaaa aaagacgagt atctactttt gtcataattt tactgttagc aggtttctgc
       61 caaaattcat aacgatcaac caatgtttgt ccacgactta ttccctgagt ttccacagcg
      121 gtgaagcttt tgaaaccttc acagatggcc ccctcgatga aataagcaac agctaacgga
      181 tcattgatga cacatcctaa aattctttct tgtttccagt ggaagtcaaa ataaaaacga
      241 gtgatcgctt ttaaatactc tccctcttca ggattcattt gacagcagta ttcgaggata
      301 gtcggtgtca agacgatttc tcttgtcaca tctaacccaa ccatttctat cgtttgtttg
      361 agattctcga atacataaga agctgcttca ggatcacacc agtaattgta ttcagcaact
      421 ggcgaacagt taccatggct tttgtaagtt ccacccatcg agacaaaacg atccatatgt
      481 ttcccaatat ttgggttgac tttcaaagca aaagcgatat tggtcaaagg acccaaagca
      541 atgactgatg tatttgtctt ttctttgaaa taatcagcca aaaaatcaac ggcatggaga
      601 ggttctgcct gttttctcaa gatcatcggg aaatttgttt cccctaaacc atccatacca
      661 tgagtgtctt gtgcactgat gaaaggttgc ttcaaaggtt tttccattcc ttgataaaca
      721 ggaatgtcta aacgatcaca gcgttccagg catttcagca cattctgcgt acctatatct
      781 gcagggacat tcccacaaac tacggtaata gcaactactt cgatatcagg ggatttgaca
      841 gctaatgata aagctaaagt gtcgtcgatc cctggatcgc aatcaataat tacttttcgc
      901 attatttcca atctcctttc tttagagata cttagttttt tattctggga agtttccgaa
      961 ccagtccaag acgagcgtct tgattataat gcacgaaatt agtatactgg cttttattgc
     1021 gaatgtaaat gctctcttac aagtaaatct attgaggaga actttaagta aatgatacaa
     1081 tgaaaaagaa atggaattta cgtgtgccga aaataaagaa tcctatggat cggtacattg
     1141 gataatagat agattatagt ttggcagaaa gcggcgggtg tacaatggtt attttttatt
     1201 tcattcttat tctgataatt atagggatca gttaccattt tctttcttat ttttatgcaa
     1261 agaaattagt aagagcggga gtacagagag gatatgactg gtatgaagac actggtcatc
     1321 gtttgatgga ccctgctgca gtcacgtcag tagaaaaaaa acgacaaaaa ttagaagaag
     1381 agtgtgaaag cttttgggag cagggaacac ccaaaacaat caaaagttat gacgggttga
     1441 aactagctgg tcagatgttc atccatccaa ataagcagaa taaatgggtc atttgcgtac
     1501 atgattaccg aagcacaggg aaaagagaca tgtctcatat aggtaaaaga tatgcagaga
     1561 aaggattcaa cgttcttgta cctgatttac gtgcgcacgg agaaagtgaa ggagaaatca
     1621 tcggaatggg atggctagat cggctagatt taattgcctg gatccaactc atcttagatg
     1681 aacagccgaa tgcgtccatt attttacacg gcggatcaat gggagcttct acgatcatga
     1741 tggcaagtgg tgaaaaactt ccatcagcgg tcaaaggatt tattttagac agcggatatg
     1801 tttctgttta tgcagaattc cgatatatgt taagtaaaat cactgtcttt cctaaaaaaa
     1861 tggtcatgag atatgcgaat cattatgccc agaaatacgc cggttactcc ttaaagcaag
     1921 catcagctac gagacagctt ggcagcaacc atctgccttt attagtcatc catggtgaaa
     1981 gagatcattt tgtccctaca gaagcagcgt acacgattca aaatgctacg gcaggggata
     2041 aagcactgct gcttgttcca gaagcagaac acttggaagc atcgatgaaa gatccgaaaa
     2101 cctattggac agtgattttt tcatttattg aacaaagaat aacgatggat taattttctc
     2161 ttcttttttg taagtaattc gaggataaat aattgtttat agatctggag actgctaaaa
     2221 taaggtagga ataaaaaaat atcagttatt tgaggagtgc ggcatatgga aatcaaagaa
     2281 gaaaaaaatc gtcttgcttt attcaatgat gagcaacaag agattggaga aatgacatgg
     2341 tcagatgcgg gcccagacat catgatcatt gaccatacat ttgtagatcc agcatatcgt
     2401 ggtcaaaagt tagctgaaaa gctagtgtat actggagtag aattagcaag acgagacggg
     2461 aaaaagatca ttcctttatg cccttatgca aaaaaagagt ttgaaaagaa accagaatat
     2521 caagacgtct tacgtcagtc ataagaataa cagctgaaag ttttgaactt tcagctgtta
     2581 ttccatttat atagagttgt cacggacttt tatctatgat aaagttaaag agacgatatc
     2641 ttttgggaag gaggctgcga tatgtataat ttaattttgg gaatcgtgat cgttttatcg
     2701 attgttatgg tcattgcgat tatgatgcag ccaagcaagc aaaatagtgc agcgagtgca
     2761 tttaccggcg gtgcggatca actattcggc aaacaaaaag ctcgcggctt tgaagctgtc
     2821 atgcagcgat caactgcagt tatgggagct atctggatga tcttattatt tgtcctagca
     2881 tatttatctt cgaagtagtt gaataagcct ctcgcaaaac ggcgggcggc tttttttatt
     2941 gcaaagagaa cgcttaagaa gaagttatga aagaaactat ggtaaaatga ggcgaaagag
     3001 gtgagacgag tgagacaatt accaaaaccc ctttatgcaa aacatggaaa gcgagcggtt
     3061 ttgctgcttc acgcttactc aggaagtcca aacgatgttc gaatgctcgc acgatttttg
     3121 gaaaaagcag attatacagt ttatgcgccg ctttttaaag gacacggaac aatggagcca
     3181 tatgatatct tgcaagaaaa agcagaaagc tggtgggcag acactaaaaa agccatccat
     3241 tttttacagt ctgagcagtt ttctgatatt gccgtattag gattatcaat gggcggcatc
     3301 tttgctgtac gagcactaga agaagaatca gtgattggcg gcggtttttt ctgttcgcct
     3361 ttgtctcctg taaaaacaaa cgtgcctgaa aactttgaaa aatacgtacg tcaagtgctg
     3421 aagacagcag gtaaatcaga gaaagaaatc aatgaaaaag cagttgctta ccgctcgtta
     3481 gctgaacaac aattaatgga tatacaagat caggcagcaa tcgtagaaag taggttgtca
     3541 gatatccaac agccgatatt tttagctcaa gctgggaaag atgaaatgat cgaccctaat
     3601 ggcgtatttg aaactgcacg caagttgagc cgtcagcgag tgacacttca atggtatcct
     3661 gaaagtggtc atgtgattac agttggtaca gcaagacgcg aattagaaaa agacgtcttg
     3721 gaatttttag aaaaattacc ttggaatgag gaataaatat gacaaaagaa acgataaaag
     3781 agaaaattct ctttttcatg gaaagcagtc gcaaacgcag tttttctatg gaagaaatcg
     3841 ctgaaggttt agggttccaa aaaagcgatg attttaaatt actagtacaa acgattgctc
     3901 aaatggaaag agaacagagt atagttttca ataaaaaagg aaaagtgaaa cttcctttaa
     3961 aaccaatctt gatcgaagga acatttcgag caaatgaacg aggatttgga tttgtaacga
     4021 ttgatcccga agaagatgac atctatatcc caaaagaagc gactggtttt gcaatggatg
     4081 gagacaccgt agcaatcgat atcatgaagc gagcaaatac agatatggat cgcggagcag
     4141 aggggaaagt agtcgagatt cgagaccggg cgactactca gttagcaggg gaattcgttg
     4201 cttattcaga agaagaaata caagaaacag atctttacgg cgttgttatc ccgaaagata
     4261 gaaagctcaa ccaatttaaa gtatatgcag cagcagaggg agttcgtcca gttgacggaa
     4321 gtattgtgct gatcgaactg acccattacc ctgaaaaagg gtatgcgact agtttagaag
     4381 ggataatcaa gcaagtgatt ggtcataaga atgaccctgg catggatatc ctctcaattg
     4441 tcgtggctaa tggtatccct acgaaatttc ctgatgatgt attgtctgaa gcagatgaag
     4501 ttccagatca aatcagcgaa aaggatataa ctggacgtag agatttaaga aatcagctca
     4561 ttgttacgat tgatggagaa gatgcaaaag atctagatga tgcagttacg gttaaaaaat
     4621 tagataacgg taattatttt cttggagtac acattgcaga tgtttcttac tatgtgacac
     4681 aaggcagtca attggacaga gaagcatatg aaagagggac aagtgtatat ttgactgatc
     4741 gagtgattcc gatgatcccg caacgtttat cgaatggtat ctgttcactg aatccccatg
     4801 ttccaaggct gaccatgagt tgcgaaatgg agattgatcc aaatggacaa gtagtctctc
     4861 atgatatttt cccaagtgtg atccaaacaa cagaacggat gacctacaca gctgtcaatc
     4921 aaatcttaga agatcaagac gaagaaacga tgcaacgcta tgctcagctc gtacctatgt
     4981 tccaagaaat gggtgaattg catcgaatcc ttgaaacgat gcgaatcaaa cgtggcgcga
     5041 tttcttttga ggatcgcgaa gcgaaaatcc ttgttgatgc tgaaggacac ccaaatgaca
     5101 tccagctaag aagcagagga attggtgaac gcttgatcga atctttcatg cttgcggcca
     5161 atgaaacggt agctgaacat tataataaat taaatctgcc gtttatttac cgaatccatg
     5221 aacagccaaa agaagaaaaa atgcagcgct tctttgattt tgcttcagct ttgggcattc
     5281 tggtccgagg aacgaaagga acgatcacac caaaagattt acagcatgtg atcgaaggag
     5341 tagaagacaa accagaagct gcagttatca atactatgtt gctaagaagc atgcaacaag
     5401 cacgctattc agaagacaat tttgggcatt atggcttagc ggcagattac tacacacact
     5461 ttacatctcc tattcgacgc tatcccgatc taatcgtcca tcgtctgatc cgttcttaca
     5521 gtgaagataa aagtgaaagc aatcaaagtt cgtgggctgc ggaacttcct gaaattgcag
     5581 atcatagttc taagatggaa cgtcgtgcag tagaaacaga acgtgaagta gattccatga
     5641 agaaagcaga attcatgatg gacaaagtgg gagaagaatt cgatgggatc atcagctctg
     5701 ttgtgaaatt cggcctgttt atcgaactgc cgaatacagt agaaggattg atccatatca
     5761 atgaattgaa acaggactac ttccattttg ttgaaaatca tttagctctt gtaggtgaaa
     5821 gaactggtct gacttttaaa atcggacaaa aagtgcgcgt aaaagtagtc aaagctgatc
     5881 cagatgaacg agcagtggat tttgaactcg tttccgcaga agaggtcact gcgttggaac
     5941 gtccaaaagc acgcaatgga aaatttggaa aacgagaagg aaaacaagca ggcaaacgct
     6001 cagaaaaaaa tggcaagaag aacaaacgat atagtgatag taagcaaacg ttcggtaaga
     6061 aaaaagggaa aaagccattt tacaaagatg tcaaaaagaa gaaaaagaaa aaataaaatg
     6121 agttggagga aacagctatg ccaaaaggcg agggaaaact aattgcacaa aacaagaaag
     6181 ctcgccatga ttattcgatc atcgacacga tggaagcagg gatggtcttg caaggaacgg
     6241 agatcaagtc gatacgaaac agccggatca atctgaaaga tggatttatt cgcgtccgca
     6301 acggggaagc tttcttgcat aatgttcata tcagtcctta tgaacaagga aatattttta
     6361 atcatgatcc gttgcgcacg agaaagttat tattacacaa gaaacaaatc gttcgtcttg
     6421 aaaacgaatt gaaaaacact ggaatcactg ttgttccttt aaaagtctat attcgtaacg
     6481 gctatgccaa ggtattgatt ggtctggcaa aagggaaaaa atcgtatgat aaacgggaag
     6541 atttgaaacg aaaagatatc gatcgacaaa ttgatcgaac attaaaaaat ttctctagat
     6601 aatttcatta tcctttttcg catattcggt gcttttgctt ttacttaatg ataaaatttg
     6661 ttagcattta atctgataaa acatgattat cacaggtttt ttcttagatc aggagcaccg
     6721 tgcatgggga aaaattcgct tcgtttttga ctgaaataaa aaagtgtcta attttcatga
     6781 aaatgttttg aattttctgt ttcatggtgg tatgataccg tgtggttaga aaccgattgg
     6841 aaaaaaccag atacgcacat gaattgattt tatgtgaatt tcggggaaaa gaggtgaaat
     6901 ggattggatg aacgctcgct aacgtttcac attggaccaa tttggtttga tggtacagtt
     6961 tgtataatgg tgttactgac ctgtttgatt gttttcttct tagcttactt ttttacaaga
     7021 aatcttaagt tgaaaccaac aggcaaacaa aatgcgatag aatgggtcat tgattttagt
     7081 cgaggaatcg taacagataa tctgcctaga aaagaattga gcaatttcca tttgttggca
     7141 tttacattgt ttttatttgt ttttgttgca aacaacatag gtttagtaac aaaaatcgtc
     7201 ttacctaacg aaacaacact ttggaaaagc ccgacagcag acccgtttgt tactttaact
     7261 ttggcattta taatgatctt acttactcac ttattcgggg tgaaaaagtt aggcttcaaa
     7321 ggatatttcg taaattcgtt cttgaaaccc tatagtttta tgtttccgat gaaactgatt
     7381 gaagaattta caaatttatt aacacttgct ttacgtcttt atgggaatat ttatgcgggg
     7441 gaagttttgc taaccttgat tgcaaaaatg atgacaagct taggctggtt ctcattacca
     7501 ttagcaattc cattagagat ggtctggatc gcgttctcct tgtttattgg gagcatccaa
     7561 gcatttgtct ttgtcacttt gtcaatggtt tatatgagcc ataagattga agtagaagaa
     7621 taatactcgg ttaaaaaaca aactactatt tatctatttt aggaggaaaa caattatgaa
     7681 ttatatcgca gcagcaatcg caatcatggg agcagcaatc ggagccggtt atggtaacgg
     7741 tcaagttatc tctaaaacaa tcgaatcaat ggctcgccaa cctgaaatgt ctggtcaatt
     7801 ccgtacaaca atgtttatcg gggtagctct agttgaagct gttcctatct taggggtagt
     7861 tatcgccttg attctagttt ttgccgttta attgaatcag tattaaggca gaaacaacat
     7921 cgtttgttac tgctttttta catggaaact agaaatactc caaagaaagg aaggctcgcc
     7981 tatgctgaat caattggcaa ttgcagaagt tggcaatgcg atgttaggta atatcatcgt
     8041 tgtcagcggc tcatttttga tactactggc tctcttgaag cactttgctt ggggaccaat
     8101 cagcgatatt ttgaaaaaac gtgaagacaa gatcgccaat gatttagatt ctgcagaaca
     8161 atctcgcatc aactcagcga aaatggaaca agaacgcgaa caacaattgt tagcctctcg
     8221 ttctgatgca gctgatatca tcaaaaatgc gaaagaaagt ggagaattaa gccgccaaaa
     8281 tattttgaag gatgctcaag aagaagcagc tcgtctaaaa agcaaagccc aagctgatat
     8341 cactgtagaa cgtgattcag cgctgaactc tgtaaaagac gacgttgcag aactctctct
     8401 tcaaatcgcg gaaaaaatct tgaacaaaga attatctcca gaaatgcatg aatcattaat
     8461 caatcaatac attgaaggtc taggttcttc aaatgaaact agataaatat acagtaggaa
     8521 gacgttacgg gaaagcttta tttgaactgg cgatcgattc aaatcaagca gaagaaatct
     8581 atcaagatct cgtaagttta cgtcaaatct atgaacaagt acctggatta gggaatatgt
     8641 taagtgatgt tcgtctagaa cctcatgaaa aacgagaaat catggacaag cttgtcagcg
     8701 gttatgatgg gatcgttaaa aactttttag aagttgttta tgagtataac cgaatggatg
     8761 accttttgtt catgatcgaa gaatatgaac gtcgatatga tgaacatcaa gggttactat
     8821 tgggaagtgt cacaacagca gtcccacttt cagacgaaca acgaatacaa ttagaaaaga
     8881 atgttgcaaa aacaatggac tatcaatcag ttgaattaaa acaaatcgtt gattcttcta
     8941 ttattggtgg cgcgattgtt gaagccaacc atcgagtaat cgacggaagc attcgcacgc
     9001 aattagaaaa aatgcgtaat caattaaaca gatagtacaa agaggtaggt gaatcgaatg
     9061 gccatcaaag cagaagaaat tagtgccttg attaaagaac aaattgaaaa ttatcaaaac
     9121 gtgctttcag tcgaagaaat cggtaccgta acttatgtag gtgacgggat cgctcgtgca
     9181 catggtttag aaaacgcgat gagtggtgag ttgcttgaat tttcaaacgg ctcatacgga
     9241 atggcgcaaa acttagagtc aaatgatgta ggtatcatta tccttggcga tttcgagtcc
     9301 attcgtgaag gagataaagt aaaacgaaca ggtaagatca tggaagttcc agttggagag
     9361 gccttgatcg gtcgggtagt caatccgcta ggtcaaccaa tcgacggact aggtgaaatc
     9421 gttactgaca aagctcgtcc agtagaagcg atggcaccag gtgttatgca acgtaaatct
     9481 gttaacgaac caatgcaaac agggctaaaa gcgatcgacg cccttgtgcc aatcggacgc
     9541 ggacaacgtg aattagtcat cggtgaccgt aaaacaggga aaacttctat tgcgatcgat
     9601 acgatcatca accaaaaagg tcaagatatg atctgtatct atgtagcaat cggacaaaaa
     9661 gattctacag ttcgtacaca agttgaaaca ttgaaaaaat atggcgcaat ggattacaca
     9721 atcgttgtga atgccggtgc gtctcaacca gcaccattgc tttatatcgc accatatgct
     9781 ggtactgcaa tgggtgaaga attcatgtac aacggtaaac atgtattgat catttttgat
     9841 gatctttcaa aacaagctgt tgcttatcgt gaactttcct tgctgcttcg ccgtccacca
     9901 ggtcgtgaag cttatccagg ggatgttttc tacttacact cacgcttatt ggaacgtgca
     9961 gcaaaattaa gtgatgattt aggtggcggt tcaatgactg ccttaccatt tgttgaaaca
    10021 caagctggag atatctctgc ttatatccca acaaacgtta tttcgatcac tgatggacaa
    10081 atcttcttag aaagcgactt gttctacgca ggtactcgtc cagcggttga tgccggacta
    10141 tctgtatctc gtgtaggggg ttctgcgcaa atcaaagcaa tgaaaaaagt tgctgggact
    10201 ttacgtctag acttagcaag ttaccgtgaa ttagaagcat ttacacaatt cggttctgac
    10261 ttggatgcag caacacaagc aaaactaaat cgtggacgcc gtacagtaga aatcttgaaa
    10321 caaaaattgc atgcaccgct agctgttgaa aaacaagtag tgattttgta tgctttgaca
    10381 catggattct tagatagtat ccctgtagac agtattttag acttcgaaca tgaattattc
    10441 gaatatctgg ataccaatca ttcagatatc tttgaaacaa tccgtacaac aaaagatctt
    10501 ccagaagaag aagcgttgaa cagtgcgatt caggaatata aagacatgtt cttagctaca
    10561 aaaggaaata attcctctac agaagataaa ttgaaatcca ttcaaaatgc atagtgaggt
    10621 gagatgaatg ggtgcttcat taaatgaaat caagacacgt atcgcttcaa cgaaaaaaac
    10681 gagccaaatc acgcgggcaa tgcagatggt gtctgcttct aagttgacaa aatctgaagc
    10741 ttcttcacag aagtttcagg tttatgcaac aaaagtaaga gaaatcgtta cgcatttgac
    10801 tgctactcag ttaaatgata ttgcctcaga aaatcctaga ggggatatca attacaacag
    10861 catgttgctc agccgtcctg ttaagaaaac gggttatatc gtcatcactg cagatggcgg
    10921 tttagtcggt ggatataaca gctcgatatt gaaacaaacg atgtctattt tagaagaaga
    10981 ccaccaatca cccgatgatt acgtgatgat cgcaattggc ggaactggtg cagacttctt
    11041 taaagcacgc ggtatcaatc tagcatatga gctgcgcaat ctatctgatc agccaagctt
    11101 tgatgaagtc cgcaaaatcg taaatatggc aacaactatg taccaaaacg aagtattcga
    11161 tgaattatac gtatgttata accatcatat caattcgcta actagtcagt ttcgtgtaga
    11221 aaaaatgctg ccaatctcag acttagatcc agaagaagca actactttcg aacaagaata
    11281 tatttttgaa ccttcaaaag aagaaatctt gtctcagtta ttgcctcagt atgctgaaag
    11341 tttgatctac ggtgcgatcg ttgacgccaa aacagcagaa catgctgctg gtatgacagc
    11401 aatgaaaaca gcaactgata atgctgctaa tatcattgat gagctgacgg tttcttacaa
    11461 ccgtgcaaga caaggggcta ttacccaaga aattaccgaa attgttgccg gagcttcggc
    11521 actagaatag tattgagatt ggaggaaaaa agatgagttc aggcaagatt gttcaagtaa
    11581 tcggtcccgt tgtcgacgtg gaattttctt tagaccaatc cttaccagat atcaacaacg
    11641 cattagttgt ttataaaaat gacgaaaata aatcaaaagt tgttcttgaa gcagcgttag
    11701 aattagggga cggagtgatc cggaccattg cgatggaatc aacagatggt ttacaaagag
    11761 gaatggaagt cattgataca ggcaaagcaa tctctgttcc tgtaggtaaa gaaacattag
    11821 gtcgcgtatt caacgtacta ggagatacga tcgatttaga aacacctttc ccagaagatg
    11881 cggaaagaag cgaaattcat aaaaaagcac cagcctttga cgaattaagt acaagtacag
    11941 aaattttgga aacagggatc aaagttatcg atttgcttgc cccatattta aaaggtggga
    12001 aagtcggact attcggtggt gccggtgttg gtaaaaccgt actgatccaa gaactgatcc
    12061 ataatatcgc ccaagaacat ggtggtattt ctgtatttac cggtgtaggt gaacgtactc
    12121 gtgaaggtaa tgacttgtat tatgaaatga aagattcagg agttatcgaa aaaacagcca
    12181 tggtgttcgg acaaatgaac gaaccaccag gtgcacgtat gcgtgttgct ttgactggtt
    12241 tgacgattgc ggaatatttc cgtgatgtag aaggtcaaga tgtactgttg tttatcgaca
    12301 acattttccg tttcactcaa gctggatctg aagtatcagc cttgttagga cggatgcctt
    12361 ctgcggttgg ttatcaacca acattggcaa cagaaatggg tcaattgcaa gaacgtatca
    12421 catctacgaa aaaaggttcg atcacatcga tccaagcaat ctatgtgcca gccgatgact
    12481 acactgaccc ggcgccggca acagcattcg cccatttgga tgcgacaacc aacttggaac
    12541 gtaaattaac agaacaaggg atttatccag cggtagatcc attggcttct tcttcaagtg
    12601 ctttagcacc tgaaatcgtg ggagaagaac attataaagt agcaacagaa gtccaacacg
    12661 tattgcaacg atatcgtgaa ttacaagata tcattgctat cttaggtatg gatgaattat
    12721 ccgatcaaga aaaagtgtta gtttctcgtg cccgcagagt ccaattcttc ttgtcacaaa
    12781 actttaacgt ggctgaacaa tttactggtc ttccaggttc atatgtccct gtagaagaaa
    12841 cagtgaaagg attccgcgaa atcttggaag gcaaatacga tgatcttcct gaagaagctt
    12901 tccgaagtgt tggccgaatc gaagacgtcg tagaaaaagc gaaaacacta ggctactaga
    12961 aaggggtgcc ccatggatca atatttaaca gtaaatgtgg taactcctgc cggtttggtc
    13021 tatgatcacc atgcagctat cgtcgttgca cgtacgacag ctggtgagct tgggattttg
    13081 cctaaacatg caccaatcat cgttccattg acgattgatg aagttcgtgt aaaacgaaca
    13141 gattcagata cccacgtcga ttggatcgct gttaatggcg gaatcatgga agttcgtgat
    13201 aatgttgttt caatcgttgc tgatagtgcg gaacgtgaac gtgatatcga tgtatctcgt
    13261 gcggaacgtg caaaacaacg cgcagaacgc caaatcgaag aagcaaaagc aaaacaagat
    13321 acgaacgaat tgaaacgggc aactgttgcg ttgcaccgag caatcaatcg tatcaaagta
    13381 tctaagcact cataaaatga gatgcggatg tttatccgtg tttaagaaca gtaaaaagag
    13441 gttatgacag aagtggtcag cttcaagaca taagaaggaa tttacgaaaa ttgcttctca
    13501 aatttttgtg aaatctcagc ttatttttcg aagaagctac ttctggaaca ccgtatatcg
    13561 gaggttgtga aatcagcgtt ttgtcctgag tattagagaa acaaatggaa aatagttcct
    13621 catattttgc cgttttttga gctaataccg agggtcagat gattgaacac cgtttatttg
    13681 gaataaggga ctgcgacaag acttttgtgg ctctttgtca ataaggactg atgagttgtg
    13741 caaaatcaaa tctgagtcag aatgaaccac attctggctc agattttttg ttatcttaca
    13801 ttgattaatt gattgatcaa aaagattcta attctcatgt tctcaaatga tt
//
LOCUS       DAZABD010000069        13647 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000069 DAZABD010000000
VERSION     DAZABD010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13647
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.069"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1233)
                     /locus_tag="ACHALG_002234"
     CDS             complement(<1..1233)
                     /locus_tag="ACHALG_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HFN2587603.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAIISL
                     IYAKSIKNNIQEIKIVTDKMMKLDKKMSLKVSSNDEVGELKQQINDLYSTLLRTIDDL
                     EFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYKERDIYINECIE
                     IVDSLTKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKITVNNYILD
                     ENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKIF
                     DVKFDLNKENSNGLGLYIV"
     gene            complement(1230..1883)
                     /locus_tag="ACHALG_002235"
     CDS             complement(1230..1883)
                     /locus_tag="ACHALG_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFN2587604.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            2171..2691
                     /locus_tag="ACHALG_002236"
                     /pseudo
     CDS             2171..2691
                     /locus_tag="ACHALG_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            complement(2689..3408)
                     /locus_tag="ACHALG_002237"
                     /pseudo
     CDS             complement(2689..3408)
                     /locus_tag="ACHALG_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010745868.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
     gene            complement(3433..3618)
                     /locus_tag="ACHALG_002238"
     CDS             complement(3433..3618)
                     /locus_tag="ACHALG_002238"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HFN2587605.1"
                     /translation="MTKALIFDFDGTMIDSEWMFYESIHHYLLKPIYWSSSAIKFLLQ
                     TINKVLGRQQNCPFMKG"
     gene            3845..5118
                     /locus_tag="ACHALG_002239"
                     /pseudo
     CDS             3845..5118
                     /locus_tag="ACHALG_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(5225..6256)
                     /locus_tag="ACHALG_002240"
     CDS             complement(5225..6256)
                     /locus_tag="ACHALG_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN2587606.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(6263..7093)
                     /locus_tag="ACHALG_002241"
     CDS             complement(6263..7093)
                     /locus_tag="ACHALG_002241"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN2587607.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(7090..7905)
                     /locus_tag="ACHALG_002242"
     CDS             complement(7090..7905)
                     /locus_tag="ACHALG_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN2587608.1"
                     /translation="MKEMKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLA
                     NYSDIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLA
                     ANFAGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYP
                     SMKEVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALT
                     SSNVNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(7902..8726)
                     /locus_tag="ACHALG_002243"
     CDS             complement(7902..8726)
                     /locus_tag="ACHALG_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HFN2587609.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(8716..9816)
                     /locus_tag="ACHALG_002244"
     CDS             complement(8716..9816)
                     /locus_tag="ACHALG_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFN2587610.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            9994..10779
                     /locus_tag="ACHALG_002245"
     CDS             9994..10779
                     /locus_tag="ACHALG_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFN2587611.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            10812..11195
                     /locus_tag="ACHALG_002246"
     CDS             10812..11195
                     /locus_tag="ACHALG_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFN2587612.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            11271..11402
                     /locus_tag="ACHALG_002247"
     CDS             11271..11402
                     /locus_tag="ACHALG_002247"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFN2587613.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            11371..11607
                     /locus_tag="ACHALG_002248"
     CDS             11371..11607
                     /locus_tag="ACHALG_002248"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFN2587614.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            11619..11705
                     /locus_tag="ACHALG_002249"
                     /pseudo
     CDS             11619..11705
                     /locus_tag="ACHALG_002249"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
     gene            complement(11685..12656)
                     /locus_tag="ACHALG_002250"
     CDS             complement(11685..12656)
                     /locus_tag="ACHALG_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFN2587615.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
     gene            13308..>13647
                     /locus_tag="ACHALG_002251"
     CDS             13308..>13647
                     /locus_tag="ACHALG_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008177227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFN2587616.1"
                     /translation="MMSKNNTNGRNQFAMLTIDDLVSQDHLVRKIDAALDFEFIYPIV
                     EATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGYSFEEKIP
                     HFSTFGKNYVR"
BASE COUNT         4127 a         2981 c         2381 g         4158 t
ORIGIN      
        1 tacaatatat aaacctaatc cattactatt ttccttattt aaatcaaatt taacatcaaa
       61 tattttatcc atattcgaaa ttttattgtt accgtatgaa ttctctatat ataaccaatc
      121 attaactatc ccaatattaa ttaccccatt tacatcagta tattttaccg cattactaat
      181 caaattagat agaataatct ttaaagctgt ttttcctata taaacattct cgtctaatat
      241 ataattattg acagttattt tcttttgatt tgctaatatt tcatactttt ttaatatgtc
      301 ttctaatgta tcatttattt tcaaatattc ttcatcatta ttcaaatttt ctatagaatg
      361 aactgataat atttgagaaa tattttttgt taaactatca acgatttcaa tacattcatt
      421 aatataaata tctctttctt tatattttcc aatattgtat ttcatgtttt ccagtattat
      481 tttaagacta gcaagagggg ttttaagttc atgggatgca cctttaaaaa agtcatattt
      541 taacttttct aattttaaaa tttctttatt tttaaattcc aaatcgtcaa ttgttcttaa
      601 taaagtagaa tacaaatcat taatttgttg ttttaattct ccaacttcat cattagagct
      661 aacttttaaa ctcatttttt tatcaagttt catcatttta tcagtaacaa tttttatttc
      721 ttgtatatta ttttttattg attttgcata aattaaagaa ataatagcag aaaataaaat
      781 tgatattaat aaagaatatg gtaaaaattt aaggcttaaa tctttcgcat ctttttgcat
      841 atcagctgta gaaacaaatt gtagatttgt ttttttacca tcattaagct ttatttctct
      901 ttcttcaatt atcaaagaat tactatcact ttctaaatta acatttatat tatctttgat
      961 ttgtatttca ttcttattat ttttttcttt tatgaatact tttatttcac tactcttgga
     1021 ataaaactcc aaagtttgtt ctatatattt tatttctttt ccattcatat tactagaaat
     1081 ttcatttgcc atattatgaa tcttttcttt tctcgtctcc aaatatgttt taggaaagat
     1141 aaaaaaaatt aatgaatgaa ctaatataat tattattcca agaacagaaa atatttgtat
     1201 gaacattttt ggaaatatct ttaatttttt cattttctct ccaatttata tcctacattt
     1261 cttatagtag ttatacagtc taattgtaat ttttttctaa gttcttttat atatacatct
     1321 attactcgat cataaggagt ttcttcgcta tctttccata cataatcaat aatttgcatt
     1381 cttgttaaca cttgtccatt attatccaat aaacatttta atacttctag ttcttttgca
     1441 tttacatcta tttcttcacc atttatttta gctgtatagc tattaaaatt aaccgataga
     1501 tccttatatt caaacttctc taaatgtcca taattcttct taattaaaga atctattcta
     1561 gcttttaaga ccggcaatga aaatggcttt tctacatatc cgtctactaa attagtaaat
     1621 gcatcaattt tatattcttc atcactaaat gcagtcaaga ttagaattgg taaattgctt
     1681 ttctttctaa tttctttcaa cacttctaaa ccatttataa aaggtatctg aatgtctaag
     1741 ataaccaaat ttatatcgct attaaatttt gacaaggctt ctcttccatc tttagcttga
     1801 ataacagtat atccaaattc tgaaagatat tcacttattc cctctcttat catattatca
     1861 tcttcaacag ttaatatttt catatatacc tccacttaag cctatacact agatattata
     1921 cagcatatta tggctctata atatctagtg ttggtgacgc cttctgttgc caatactttt
     1981 tctttttttt aataaatgca aacagacacc ttgaaatcct ttagaaaaaa ggcttctata
     2041 atattttgtg ttgttggaaa atcgaaggcg attttctact aacacttttt cttcgtttca
     2101 actcaaaaag agactcactt ttggtaaaat ttagtcgacc aaaacatcac cctaccaaag
     2161 gagtcgctct atggataatc atactagaaa attactcgga ttaacagata aacatttatt
     2221 ttttgatgaa gaatggctga ttgaaaaaga atataaaggc gttcaagcgc agtttattaa
     2281 agggagattg gatgactcac cttcccattg tgaacactgt ggatcgattc gtattatccg
     2341 caacggttcc tatacgacac gtactcagat gttgaaagtc aaagaaaagt tgactatttt
     2401 agaattaaaa cgtacccgtt ttctatgcta tgattgtggg cagacctttt ctgcgaaaac
     2461 ggacttagtt gatgaacatc atcaactaac aaaggaactg aagcaggcga ttctcataga
     2521 actgtacgaa aatcaatcac gtaatctgaa aatcagaaga agtaccgtcg gctcaagcga
     2581 tattggaaac tcttactcaa agattccacg acattggagc cgctcaaacg gcattatcat
     2641 cgactgttta aacgaccaat tagtcaaaca gaaatcgtcg atgaaccgtt gtgcacaatg
     2701 gtgtcctgaa cgtacagcga caccttccat atccagagca gtcgccacat cacggggatg
     2761 gatgccatcc aaattaaaag caaccacgcc agtatgatgt tcaggatctt gcggtccgta
     2821 aatggtgata ccgccgatcg cttgtaattt aggtaaaacg taggcaacca gttcttgctc
     2881 ataacgatga atattctcca taccaatctc attcaagtaa tcgattgctc ctgcaatgtt
     2941 cggagtacca gcttcaaatt tccatggcag ctctttccat gaactatcgt aaagattgac
     3001 aaaatctatc atttcaccgc cgaattcgac tggctccatt tgttctaaca attgacgttt
     3061 gccatataat acaccgattc ctgtcggccc gcacatttta tgcccgctaa aggcgaaaaa
     3121 atctgcatcc aacgcttgta catcaactgt catatgcggt gctgcttggg caccgactgg
     3181 actatcctca actacaacag cctgttcaac cctcagccgt ttcaagcaca acaaataacc
     3241 ttctggatct ggtttgccga cctgaagatc atcgatcgtc acaaaggaat caattttttc
     3301 cttaatcatc ggatgtgctt caaaaaaata gtctagctcc cgcagttcag aattgagact
     3361 actccgattt tccagtgctg ctgatccgct gctcaaaatg ttcagcatcc actggtctcc
     3421 caaccgcttg acttatccct tcataaaagg acagttctgc tgccgtccca acaccttgtt
     3481 gatagtctgc aaaaggaatt ttatagccga actgctccag taaataggct tcagtaaata
     3541 gtgatgaata ctctcgtaaa acatccattc actgtcaatc atcgtcccat caaaatcaaa
     3601 aataagtgct ttggtcatct tctttccccc ttacccaaaa caagcaaaag ctgtgtcctg
     3661 ctctctctac agtatcagca taaaaactgc cgtctgaaaa gtcaaaggtg aaagaaatgt
     3721 ggcgctataa tttctaggac tgatagtaaa tcattctatc agtccttttg agcataaaaa
     3781 aacaaaacat acagaagctc cgttagaatt gaatcaccac aacacaatca aaggagcgtt
     3841 ctgtatgcct gatcttcatc ttatcaaact attactagga atcaaagatg gaaacattca
     3901 aatcactcat gtcgagaaga aaaaaataca ttcaatcaac agtatcgttg tttctgccac
     3961 gctcattaaa acgattcagc cctgtcctca gtgtaaacag gtgaatcaac atgggctgat
     4021 cgttaagaac ggaaagaaaa aatcgctggt ccttctcaat aagtgtgcga atcaacggac
     4081 ttatcttgct ttggcaaagc aacggtacct ctgtcgtggc tgtcacacct attttacggc
     4141 gaatacctac attgttgaaa aaaactgttt tatcgccaaa caagtacatt ataaaatatt
     4201 ggaggaattg actgaaaaac aagcaatgac gacgatttct aaacattgcg gcgtttcttg
     4261 gtcaaccgtt tcgcgaacac tcgcttctct gctccccatg acaaacgtga aaataaattg
     4321 gttgcctcgc tgttcattgg tagatgaatt tcgttcattg aaaaatcaag tcggtcccta
     4381 ttctttttct tgtatggatg gcgatactgg gaaattactg gatattcttc cttcacgtaa
     4441 gaagaaagat ctcatttctt attttatgca atttgaacga cgtgcacgtt taaacgtaaa
     4501 aatactcgta acagatatga acgcctctta tgctttgctg ataaaagagt gctttcctca
     4561 tgccaaactc gttgtggatc gctttcacat cgtcaagcac ctcattcgca gttttaagga
     4621 tatttgacta tgagtcatga aaagcttcga ccgaaacgat ccaatacaag ccaaacacta
     4681 tcgacaagtg aaggctttat ctcgacttct gatcaaacga caggacacgc ttgtttatga
     4741 taaatggaca aaatggcgta attttggttg ggcctattta accgaaagtg aagtcgtaga
     4801 acgtctactt tctacttctg atgaactacg aatagcttat gcttactatc aagaaattct
     4861 tcaagctttt tatgacaaag aagcggacat cttctttcag cttgtaaaaa caatgcccaa
     4921 gtcggttcca agagaactgc atcatataaa aaaagcgttt ataaactatg aatccggcat
     4981 tcgtttagcc ttagagttgc cttactcaaa tggcaaaata gaaaatctgc atacccacat
     5041 caaagcattg aaacgaatag cttacgggtt aggatgagaa ccagaatttt cttaatcaat
     5101 catctgatca cttattaaac ataaaaaaac atctaaagca gaaaatcaat tctaccttag
     5161 atgttcttat atcactctat cagtcctatt tgacaaagag ccagaaatgt ttgccgacta
     5221 tacactagct ttgttgtgca aaatgctgag tatcaatttc cacagggatt gctagaatat
     5281 ctttcagctg aatgtataag gcatcgctaa ttttctcttc ccgatcgttc aaccgatcca
     5341 ctaaaatttc tttgccctcc agcgtttgaa aaacataccg ttgaatgttg cctaaaataa
     5401 atgattcctt ccatagaacc ggaatcttaa ccccctgagg aaccggttca aattgaatca
     5461 gctccggtct taaataataa atgcagcgat tgcccttagc ggaaaactcg tgggtatact
     5521 gattcagctc ataccattcg aaacgattat agttgccgat aaattccgca acaaatcggc
     5581 tctgaggatt ctgatacaat tccttcgagg tggaaacctg agccacccgc ccagcttcca
     5641 tcacaaaaat ccgatcactg attcgcatcg cctcttcttg gtcatgagtc acaaaaatca
     5701 tcgtcatccc tagcgattgc tggatagcgc gcaaatcccg ctgcagctgt tttctaattc
     5761 gggcatccaa agcacttaat ggctcatcca gcagcaggac tttcggttca gtgaccaatg
     5821 agcgagccag tgccacccgt tgtcgttgac cgccagaaag aaactggaca tttttatctg
     5881 cctgctccgt catatccacc aatgatagca tctcaaaaac cttttcctga atcacctcag
     5941 gactcagctt ctttaccttc aaaccaaagg ctacattttc aaaaacagtc atagttggaa
     6001 acaatgcata ctgttgaaaa acaaagccga tattgcgatc cttcgtaggc ttatcggcaa
     6061 tattctcatt gtccagcaca atctcaccgc tctttggttg ttccaatccc gtgatacagc
     6121 gcagcaacgt agactttccg cacccgctgg gacctagtaa agtaaccagc tcaccctttt
     6181 caatctgaat ttcatcaata gctaaaatct gtttttcatc aaaaaacaga ttgagatcat
     6241 gtatttctaa aaacattttc cgtcacaact cctttaaggg cttctttttc gactgcttct
     6301 cttccctcgt ctgcttaagg gacagctgtt gcttttgtcg ataagtcagc ataaaggtag
     6361 ttaaagcaat tcccaccata aacagcaaat acaaaaccat cacagcgcta gccaaatggc
     6421 cgttttcgtt catccgccgc agcatataga tgcgtaccgt ctgaaatcga ccgccaatca
     6481 acaggttcgt cagcatgtac tccccaaaaa ccgaagaaaa aatcatcaga gaaactaggg
     6541 tcacaccgat acggatgttt ggaaacagca cctggataat aatcgtagac atcggggcgc
     6601 ccaaggtttc tgctgcatcc actaattcct tcagattaat taagcgcatc tggttgttaa
     6661 gactaagata gacaatcggc aacgccgtaa taaacaatgc acctaccaac actacaaaca
     6721 ttgggatacc agtttcagca tacgttcgaa gcaaagccgt taccaaaatc actcctggta
     6781 aagcatacgg cagcaggagc agcttttcca gccaacgatt caaacgaggg aaatacaaat
     6841 gaatccaaat aatggtggga atcatcaaca acagaatcgt caccaagacc acacctgcta
     6901 ataaaagtga gcgacccacc gcagcaataa attccctgtc cgtcagcagc tgttggaacc
     6961 attgcaaggt aaaatcacta ggtaaaagcg attttgacca tttagtggag ctggcataca
     7021 aaatcgttcc aataatcggc agcaagtaat acaggacaaa gcagaacaaa atcggtttta
     7081 ttaacttttt cacgtctgca ccaccttgcc tttcttttga ataatcaaca ttgccgttac
     7141 cagcaggaca cctaaagcga ctgccaacgc actgcctaaa ttaggcttgg cgaacacatc
     7201 tcccgacact aaagccgcaa ttcgaatcgt taaaagattt acattactgc tggtcagggc
     7261 atacgctgtt tcataggtcc ccatgccatt agcaaataag atgacaaagg tgctggcaat
     7321 cgatggtcgc aaaaaaggca aagccacctt gaaccaagca tacgctgatg aagcgcccag
     7381 caattgtgcg gactccagcc attccttttt cacttccttc atgctgggat acaaaaacaa
     7441 gacccctaac ggaatttgga aataggtata cgtgatggtc aatccccacc aagaatacaa
     7501 attgaagtcc gctagaaatg gcaaaatcat tttcagcaca cctacattcc ccaacaaaat
     7561 aataaaagag aatgccagcg gaatcccagc aaaattagct gccaaattgg aaacaaaggt
     7621 gattttctcc tgcaatttag tcgatagatt cattaagcat aaacataaaa taaacgtccc
     7681 gattaaccca atcacactgg agctgacagc aatcagtacg ctgttaacaa aggcctgtgt
     7741 gtaatatttg ttgctgaaaa tatcactgta attggccaat gtccactggt tagttaaatc
     7801 actgtgcaag ctgcacagaa ccataaaaag cagcggcagc agtaaaaatg cacagaagat
     7861 aaaggaggca ggaaccagaa ccaagcgttt tttcatctct ttcatcccct catcaattct
     7921 tgaatgtcct gcattttctc tgacggtgca attcctaata accagcaaaa caatggagcc
     7981 gccagcagct gtggtacagt ttgttccttc agcttttggc gtttatttga aatcaaataa
     8041 aaaggaactt cacgatgtgc agccaagctg ccgccatgca gtccgtgcgc atccatcccg
     8101 tgatccgcag tcaccacgac ttgataacct ttttgcagcc attcaggaag gtaaatagcc
     8161 aataatctgt ccgcctgatt aaccgctgct gcatattcat gagaatcggc ggtaaatttg
     8221 tgaccgacat catcaatatt catcgaatgt acgaccaaaa agtctggctg atactgattc
     8281 actaaataat gggcatccgc aaataaatgc gaatcagggt aagtgtcctc actataaaag
     8341 atgccatgct gaatcagtcg ctcctcctga tgctggatgc gatcaaccaa acgattatag
     8401 ggggcttgat tgtacaactc gctgtaccag tggtaagcgg cggctcctgt ttttccgcca
     8461 gcttccttaa ctaattcaaa cagcgaccac tctttggatc gctgagcatg gtaattacta
     8521 tagatgccat tttcataggc tggaacgccg gtcatcagta cttcgtataa cggacgtgaa
     8581 ttgctgggca gctcactttt taccttcaat aagcttgctt gctggtgctc aactaaatga
     8641 ttcatgaacc caagctgggc gacggctgtg tcgtagcgac agccgtctag tataactaca
     8701 atcagtttgt caagtctact ttgcataggt cagtacctcc gcctgccaaa gctctggaag
     8761 ctctacagag gtcttgttcc aagcttcatt gtcttcaact ggcaccgccg cttcataagc
     8821 atcctccggc agtaattttt cagctacttc tttaggcagc tctaccttgc gaataggtcg
     8881 cgcatagccc tttgccaaat tgatttgccc ttcatcagat aaaatatatt cccgtgctag
     8941 ctttgccgca ttgggatgag gggcattttt attgattaag gttgtatacc cgctaatgat
     9001 ggagccgtct gttggcacag ttacctcaaa acgctcggag tcaatttggt cacgataatt
     9061 tagtccattg aagtcccaaa caactgccac atcgatttca cctttttcaa gatttgctac
     9121 gctggcatca acggtggaca aacggccttt ttctgccatt tttttgtagt aattcagacc
     9181 tggctgaata tccttttcgt cacctccgtt ggcaatcgcc gcagccaata cagcaaattg
     9241 tgcttggttg gcaatcgtca catcccccac gctaacttga tagtcaccct cttgcagctc
     9301 cttccagcta gtcggtgctt gcttcacatt ctctttatcg gtgataaagg aaatggttcc
     9361 cgtatagctt aacaaccaat ccccatcatc atcctttgcc caatccggaa tttcctccca
     9421 atagttggtc ttataaggta aggttaactc tttgctttca gccaacggac cgaagctgat
     9481 accgacatca ccaatatcgg cggttccatt ttttccttct gattcaaact tagccaattc
     9541 ttctgcactg gacatatccg tatccacgtg acttaagccg tattcttttt caaggtcgtt
     9601 ccaagtgcct acccaatttg cccaagtatc cggcattccc aagctggcaa tctctccttc
     9661 ttccttggct ttttctataa tcgtatctaa agagtcgtct gccgcagccg attgcttcgt
     9721 tgcttcacct gagccgcagc ctgtcagcag gatacttact gttgctgcac aaaataaacc
     9781 gatagtcatg ttacgaattt tttcattcat tttcattcat tcttctcccc tttcgataat
     9841 tccattctaa caaagctttg taatgagaga atcagtttct tgaaaaggtt tcatgaacaa
     9901 tgtaaaggtt gtatatggtt tgtaaatttc gacgaattcc cgttaacctt ctaaagcttt
     9961 tgcggtatga tagtagaaaa aaggaggctc cctatgcagt attatgatca gcatctccac
    10021 acgtattttt cctttgattc agaggaaaaa tttgaaaact atttagccta tcagccggag
    10081 tttttcgttt ctacggatca ctttgattta aacaatccat ctaccggaaa agatgacatt
    10141 ccggattatc cagcctatat aaacaaactg gactctttaa aagaaaccta ttccaccaaa
    10201 tttctgcgag gtatcgagat tggcgttgtt ccaggacaag aagagcggat cgtggattac
    10261 ctcgctcagc atccttatga tttaaagctg gtaagtattc atcaaaatgg ccgctttgat
    10321 tatatggacg atgtggtttt aaccaaagat cccattcaaa ctaccaagga atattttgat
    10381 cagatgttgc aggtcttgac ggattttcca gaaggagata ttttgactca ctttgattat
    10441 ggattacgcc gcttcacctt cacagcggaa gaattcgcgg agcattttga ggctcagctg
    10501 ctaacgattt tcaaaaaggt tgttgaacga gaattggcga tggagttaaa tgccaagagc
    10561 ttcttgaaat acggcaacga agacctttac cgttatgccg ttcctttgta tcaaagtgtc
    10621 ggcggaaagc tctttacctt aggctcggat gctcatgttg ccgaagatta tcagctgggc
    10681 tttaaaaaaa tgagtcagct gttggcagac tgtcaggtag aaaagttgct agtcattcaa
    10741 ggaaaagaac gccgcctgac gccgctgccg attttgtgat tcctatataa tagaagaaag
    10801 tggtgaaaaa aatgagcaac ctgtgggact acaatcaaga ggcacccatc cattatttaa
    10861 ttgcgcgaca ttgggatgcg ttgaaaattg aggcggtttg ccgatccttg ttagcagccg
    10921 ttccaaaaca acaactggaa aattttttgg tggctgacag cttgcagcgg gaaaaagttc
    10981 aggcttattt tgcagccttt aaagatcagc ctttggaata tttgcatgcg cagtttcatc
    11041 ttttttatca agtagccgcc ccagatgact acaacgattt acgaggacag ctgcaattga
    11101 cctttcaagc ggatgaaacc gcctataccg ttttattggg aatggctcgc cttggtgatc
    11161 aggcgaaggt cgagtggcgg attttcgata tttgatggca ggatcaaggc gtttcagttg
    11221 caaaccttcc tctctttcaa ttagaaaaac cgcagcagga agaagtttgt gtgctggtca
    11281 ccgatgctgg tgaaattcgt ctccgcttat ttcctgacca agctcccctg gctgtagcca
    11341 actggcgctc cctagctgag caaggcgttt atgacaacac acctttcggc tcgtgtcatt
    11401 aaggaatttg ttattcaagg tggcgctttg gatggttctg gtgatgaagc tgtttccagc
    11461 tatgagggct ttttcgctga tgaagtgcat cgcggacttt atcattttaa tggcgccttg
    11521 gccttagggg accacggccc ccacaccaac ggcaaccagt tttttattgt gcaaaacaca
    11581 aaggcgcagg ctgacctgct aatgtaaagc aagcatactt ggagcttgga ggactaccgg
    11641 agctggatgg tcgttatact gtttttggac aggtatacca agggttagaa atcgtagcag
    11701 cgatcgcagc tcagccgaca aacagtgaag atgtaccgat tgagccagtg ttcattcgac
    11761 aaattaactt cgaaaaaaca tcgtaaaaag acactcactg acttcagcaa aagctctatt
    11821 cttgagctgg ctgcagctag tgagtgtctt tcatttatcc ataagataaa gcttggattc
    11881 aataaataaa taaatgtttt ggttaaagct gtgaccagca aatgaggaac cgtgcgagcc
    11941 agttgatctg ccaatgcaaa catttcttta ggtgaaggcg ctggcgtgtc tgccaacgga
    12001 tactccagtc ccaatgcttg gtaaccgtgc ataccatagc agtggtacgg tagtaattct
    12061 aacggtacga accgatcaag atctgccaca aaacaagcaa tcgtcagcag ctcggtcagg
    12121 ttcatattga tacctggaac cacaggaact cgaattcgca cttcgcagtt aggatactca
    12181 attaaaaagc gacgaatatt cttcaaaatt tgttggttat ctaagccggt ccattgtttg
    12241 tggagcttgc tggtaaacat ttttacatcc acaaacaatg tatccacata gggagctacc
    12301 ttttggattt cattataagc accacaaaag ctggtttcca gtacggtatt gattccaatg
    12361 tatttcgatt tttgcaaaat ttctttagca aaatccgcct gaaccagtgc ctcgcctccg
    12421 ctaatggtca ctccgccatc cgagtgaaag taaaaaacag catccttctc aatctcatcc
    12481 attacctctt cagcggtcat ggtctggcca aatccttttt caatctgctt gctttgtgat
    12541 tcgggaatcg aacaccaggc acagcgtaat gggcagccct ttaaataaac aacggtccgt
    12601 aaaccagcac catcatgtag agaaccctgt tctattctta atactgttcc ttccatatag
    12661 atttttgaat tacacactag tttaaactaa aacacacaag gaaaaactta ccatttaatc
    12721 ataaaattag ctcatataaa tatactagga tgtaaaatag actggtcaaa atctggtcaa
    12781 aaaagaggac taatacaggt agtcctcttt tttgttattt taatattcta aatcattttt
    12841 tattggttta ttcccttatt cgatgtagac tcaaatcact atatcactag atatcaaacg
    12901 gcgtcatacg gaatcaaaaa catcaaattt gacctgttaa atggtcattg aataacattc
    12961 aaaatcaata aaacggagtc agacacggag tcaaaaacgc tgagagtaaa aaatatatac
    13021 agccttgtag gtaaagtacc acagggctgt atatttttta tttcagatta ccttaaatga
    13081 ttccatttac tttttatctc attttttcct actagttagt atcaaatact ctcctatttt
    13141 ctcaataaac tatttttgga tacatggtat ctgaaacagc taaaaccttg tcattacagc
    13201 aaaaaatcac gttatccaga ctgtagacaa agtccatttt atggatgatg tctacagtct
    13261 tttcttttat aatagattta tgaaaacgaa tgcaaggagt gatagctatg atgtctaaaa
    13321 acaatacgaa tggtagaaat caatttgcga tgcttacgat tgatgatcta gtttctcaag
    13381 atcatctcgt tcgaaagatt gatgccgcgc ttgattttga atttatttat ccaatcgtcg
    13441 aagccactta ctccgattta ggtcgcccaa gtatcgatcc agtgattctg atcaaattgg
    13501 tgtttattca gtatttattc ggtatccgtt ccatgcgaca aaccattaag gaagtcgata
    13561 ccaatgtcgc ttaccgctgg tttttaggct actccttcga ggagaaaatc ccccatttct
    13621 caacttttgg aaagaattat gttcgcc
//
LOCUS       DAZABD010000070        13410 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000070 DAZABD010000000
VERSION     DAZABD010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13410
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.070"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            135..308
                     /locus_tag="ACHALG_002252"
     CDS             135..308
                     /locus_tag="ACHALG_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587617.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
     gene            948..1421
                     /locus_tag="ACHALG_002253"
     CDS             948..1421
                     /locus_tag="ACHALG_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HFN2587618.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1430..1657
                     /locus_tag="ACHALG_002254"
     CDS             1430..1657
                     /locus_tag="ACHALG_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN2587619.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            2293..2664
                     /locus_tag="ACHALG_002255"
     CDS             2293..2664
                     /locus_tag="ACHALG_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587620.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(2920..3162)
                     /locus_tag="ACHALG_002256"
     CDS             complement(2920..3162)
                     /locus_tag="ACHALG_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFN2587621.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(3194..4096)
                     /locus_tag="ACHALG_002257"
     CDS             complement(3194..4096)
                     /locus_tag="ACHALG_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFN2587622.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(4109..4297)
                     /locus_tag="ACHALG_002258"
     CDS             complement(4109..4297)
                     /locus_tag="ACHALG_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFN2587623.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(4311..4874)
                     /gene="amaP"
                     /locus_tag="ACHALG_002259"
     CDS             complement(4311..4874)
                     /gene="amaP"
                     /locus_tag="ACHALG_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFN2587624.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(4912..5805)
                     /locus_tag="ACHALG_002260"
     CDS             complement(4912..5805)
                     /locus_tag="ACHALG_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFN2587625.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(5883..6806)
                     /locus_tag="ACHALG_002261"
     CDS             complement(5883..6806)
                     /locus_tag="ACHALG_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFN2587626.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(6855..7205)
                     /locus_tag="ACHALG_002262"
     CDS             complement(6855..7205)
                     /locus_tag="ACHALG_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFN2587627.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(7238..8140)
                     /locus_tag="ACHALG_002263"
     CDS             complement(7238..8140)
                     /locus_tag="ACHALG_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFN2587628.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(8133..8990)
                     /locus_tag="ACHALG_002264"
     CDS             complement(8133..8990)
                     /locus_tag="ACHALG_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFN2587629.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            9324..10133
                     /locus_tag="ACHALG_002265"
     CDS             9324..10133
                     /locus_tag="ACHALG_002265"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFN2587630.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            10173..10670
                     /locus_tag="ACHALG_002266"
     CDS             10173..10670
                     /locus_tag="ACHALG_002266"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HFN2587631.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(11055..11128)
                     /locus_tag="ACHALG_002267"
     tRNA            complement(11055..11128)
                     /locus_tag="ACHALG_002267"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11092..11094),aa:Ile,seq:cat)
     gene            11315..11638
                     /locus_tag="ACHALG_002268"
     CDS             11315..11638
                     /locus_tag="ACHALG_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587632.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            11802..12056
                     /locus_tag="ACHALG_002269"
     CDS             11802..12056
                     /locus_tag="ACHALG_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587633.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            12126..12371
                     /locus_tag="ACHALG_002270"
     CDS             12126..12371
                     /locus_tag="ACHALG_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN2587634.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            12447..12812
                     /locus_tag="ACHALG_002271"
     CDS             12447..12812
                     /locus_tag="ACHALG_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02328 family protein"
                     /protein_id="HFN2587635.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(12873..>13410)
                     /locus_tag="ACHALG_002272"
     CDS             complement(12873..>13410)
                     /locus_tag="ACHALG_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587636.1"
                     /translation="VISFFKEYLEIINFRLKTKKESVIILVYFQGGNLMIKKLFLLSI
                     LTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGILGRSNCKHRC
                     SNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREVGDEPKLEYA
                     FSFAREDEDNPKTYDILA"
BASE COUNT         3909 a         2477 c         2244 g         4780 t
ORIGIN      
        1 tttcggagaa ttaaaaaata caataaataa ctttagttac tttaatgcgt tcttacttta
       61 tttttatatt atcatttttc ttattcactt ctttggttta tactattctt ataaataaaa
      121 acgaggtatg tcctatgatg aaaagattgt actattcatt gatcataacg attggctatt
      181 tgattgtatc aaatctagga aatatggtat ttggtatcag taaagaattc agttggacta
      241 ctacattatg ggaatctcta ttctttttca tcttcgtatt tttgttacaa aattatcgaa
      301 aaaaataaac atgtgccatc tatctgttgg cacatgtttt aggatttatg gtacttttta
      361 ttcggcaatc cattttattt ttctacttct tcttaatacc tctaacactt ttgatttata
      421 aactaatcca atgcatctac ttgcgcttat taagctattt cagctagata gtaccacccc
      481 atgcgtcaat gcttgatttt ccttttgtat aacattgtat aagcaataaa ggacaaataa
      541 gtggtgatta caccaaacca ttgaataaga aaaattctga tacgtaaata aacatgaact
      601 tgaaaaaatt aatgaatcgg ttagctacta cgtagttgtc ttttttctca tacagttctg
      661 ttaacttaca cttctcttcg ctagataata aaaaatcctc caactgtatt gtccaatata
      721 atcccaggca tctaaactag cattatagaa gagacacaca tagaatctaa aataactggt
      781 tttaaatgtt tcttacaatc aaaaaaagct gttaaatgca ctccacacct tcatactcta
      841 tcatttttta taaacgatat atttttattg tttttcgata aaaaatagtt gttttacaat
      901 aattattctg ctatatttta gctatcaaag ataaaagagg gataaaaatg aaatttaaaa
      961 cattgctatt aaaatttgtc gttttaggta taagtgccgt atttattttg ttcggcgtac
     1021 ttttttatac tcagatcgct acaagcgaaa aaggattcaa ttttgattgg cagaccacat
     1081 tatttgtttc tgtcatttac tttgcccttc tcctagggat cgttattgct tatttcttat
     1141 tgaaactatt gaaaaaaatc gaagataacc aagctttttc cactcaaagc ttaaacatac
     1201 taaaaaaaat cgaacgaacc attttgttta ttggtttcgc cttttttggc ttacttccta
     1261 aatttatgaa agtagcggat ttagacgatg ctccaggttt gatgttggta ggaatcgctg
     1321 tcgtattcat tccttttgca gtatacacat taacaagcgt gctggaaaaa ctgcttgaac
     1381 atgcgatttt acttaagaca gatcatgatt tgacagtgtg aggtgcgaca tggcaattat
     1441 cgttaattta gatgtagtat tagctcaaaa aaaaatgagt gtgactgaat tgagtgagaa
     1501 agtgggcatt acgatggcaa acatctctat tctaaaaaat ggaaaagcaa aagccatccg
     1561 tttttccact ctcgaaaaaa tctgccaagt tttggagtgt caaccaggtg atttgttgac
     1621 ctataaaaat gactcgaata ctataccaga agaataaaat aaagtagaac aaacagtaaa
     1681 taaaaaaaaa tctgaatgat agatgtgtga gacttttaga agttatttac gtctattatt
     1741 cagatttttt atttcgattg aataccgttt tatctataaa taagatatgt actgactcat
     1801 acaaaggggg ccttttcttc tccatgtact atcacttttc ctgatacaac tggtttcctg
     1861 ttacgttgta gttggatctg cttttagtat aatactttat tcattccacc agactgatag
     1921 cagtttgagc agtctaaata atcatgtagt attacaggcc attctgtttc ataccttgaa
     1981 gtgcttaaaa cttcagctaa tggcccattg tgttaactct tactccattc ttttttagta
     2041 agctcccata tatactgtaa agccttctat atattatgtc atgttgcttt ttaacattta
     2101 ctatgtagtt ttgataaaat gatttgaata acagtgatgg acaattcttt tttattttta
     2161 acattatttt gataataatg attatttatt taaaaataca ttagataaca tttgttacga
     2221 cacgaaaaag cttcacaaac aaatatcgtt attttaaaat gagggcgaac tcacacaatt
     2281 aggaggaata gtatgccatt tattgtttac ttttttcttt cattactcgt gatctatatt
     2341 ccattaccaa catttttcct gctcgtcaat cgtctagcag agcaacatga tacgattgct
     2401 gctcttacga aactttgttt acttctattt gtttgtctag attactttac gagctattat
     2461 tggctaaaac agacaagagt aaaaaagaga tattatttct tccttgtctt tttcaatctc
     2521 tttgagtttc tgatccattg ctttttactt catcagtatc atacgatcca tttgtggatc
     2581 atctgggttt tcaaagtggt cgtattattt tgtatgttca cctttcctat ctcaagaaaa
     2641 tttagggagg cattgtttta ttaaagaagc ttctctgtat caataaagac acatttaagg
     2701 aaaaactttt aaaaatctaa ttgggaattt gcccgcataa ataagaggct gtgacaaaag
     2761 tcttgtcaca gcctcttatt ccgtataaac agtgttcaaa agcttgcatc ttcgataata
     2821 tgcccaacca aatgaaaaat atgagaagct atttttgttt ggctgttctt attacttgat
     2881 gaagaacagc tttttcacga cctcttttta aaagtctttt tacagttttc gagcaaagaa
     2941 tgatacgatt agtactaaaa tgatggcacc aatcaatgat ggcacgattg ccattcctgc
     3001 aatacttggt ccccaagaac ctaataatgc ttgaccaagt gctgatccaa ctaaacctgc
     3061 tacgacattt gcaataatcc ccatcgaaga acctttattc gtgattgctc ctgcaattgc
     3121 tccgataatt cctccgacga taagtgacca aataaatccc atgtttcgtt cctcctaatg
     3181 ttttacttcc ttattactga acccgtggtt ctgtattttc ttttactttt tgtgcgccgc
     3241 ttccagctgc tgattttact ttatctgttt gattcgaagc aaattcaccg gtttttttcg
     3301 ctgcgtcagt tactttgtct tgcaccgttt cttgatcttt ttcaaattct tcttttgttt
     3361 tgatgtccac tacgttagcg ttgacttcga tcacttctaa atgtgtcata ttttttactt
     3421 ctttgccgat tacttcttgc atttcttcaa agattttttc gatatctttg ccatattcca
     3481 caatgacatc catatcaact gctacttgtt ttttaccgac ttctgtttcg atcccagccg
     3541 ttacattatc tgtattcaca agttttcctg ccacattaga gaagaaacca ccgtttgcag
     3601 ataataaacc gtctacttgt tcaattgcga ttccgatgat tttttgcaca actttatctt
     3661 cataattcag ctcaccgcgc attgaatctt cctgttcaga atcatgcttt ccttggttat
     3721 ttgtatgatc agaaccaagt tttccttgtc cgtttgtgtg gtcagaacta agtttttctt
     3781 gtgctttcgc atggtcagaa ggatgttttt gctcttccac aggtttagaa ggatcgattg
     3841 catcatgttg ttctggaact ttagattctg gagttcctgg atgatgaggt gcatgctttt
     3901 ctgcatcatg tttcttctct tctactcctt gttgaacgtt agggactact ggatctggat
     3961 gtgcagatac tggtgctgtt ttgttctttt catcgttcac accatttgat tggtttggtg
     4021 tcgttgggtc tggatgtgca gacactggtt tttggttgtt cattttattg ttattatttt
     4081 tgttattgtt attcatggat gtttcctcct aagatttaaa atagtgatct aagattttcg
     4141 tttgtttcag ataaaaaccg atagcgacgc cgatcgctgc catgatcaag atcaataagg
     4201 tcttgaaaaa gcccacggtg atccataaaa cggctaatat caatccaagt accccaaaga
     4261 tgatagggag cttgtatgtc gaaaatagtt ctttcatttt tggtcccccc ctattctact
     4321 cgtttgcggt cattgcttcg tttagctggt tcttcaaagc ctgtgtattt caccgatact
     4381 ttcactggct cttttgttcc caatatgtgt tgtacttcat tttcgatttc tttcatcaat
     4441 gttcctgttt taccaatgag tgctgaagtt cgtttcaact ccccttttac attgacggcg
     4501 atcttgtttt tcgttgcttt gacatttact tttggtgaat ggatgaattc atcttcatgc
     4561 aagtgagaac gaaccattcc ttcaattgct tttttatcca acgtcaactc cccatcacca
     4621 tgtttcagta gaaagtttcc gattgttttt ggataaaaca ggaaaacaag taacgtgata
     4681 attgtcaaaa tagcaaatgc aacggcaagc caaaatacat attgttgcac gtatgaatta
     4741 gtgatggaaa agaatcgaaa cttctctagc tgaaaaggca atggataggt tgcacttaat
     4801 agtatcgcaa acaaaagcac agatagcaaa agtaatgttc cgatcactcc gatcgttttt
     4861 acacctttat tcattttcgc acctcctcat aggggtgaaa cgatcaactt cttataagtt
     4921 gatcgcttct ccaccagtta ctccgtaaat ttgggctgta acataacttg ctttattcga
     4981 tgctaagaaa acataaactg gtgctaattc tcctggctga cctgcacgtt ctagtaaact
     5041 ttgctgacca aattcaggga tcgaatcaga aggttgtcct tcgtctagtt gtaatggtgt
     5101 ccagattgga ccaggtgcta caccatttac acggattcct ttaggtgcaa gctgttttgc
     5161 taaaccgatc gtaaagttag cgattgaagc ttttgttgct gcgtaatcta ataaatgttc
     5221 gcttggattg aatgcttgaa cagaagttgt tgttacgatt gctcctcctg ctgggatatg
     5281 cggaacagct tctttcacta aagcaaacat agcgatgatg tttatcataa atgtgtcttt
     5341 gacttgctca agtggtaact cagcaataga ctgacaagaa atttgctgtc ctgcgtttag
     5401 taccatcgta tctagcccgc caagttcact cacggcttta tctatgattt ctttaggtgc
     5461 ttgttcgtca cgaagatcaa aaggtagtaa gacaatctta cgtcctgcat ctttcacgta
     5521 ttttgctact tcctctgcat ctttttcttc tcctgggaag aattgaagag cgatatctgc
     5581 cccttctcta gcaaatgcaa tcgctgctgc acgaccgatc cctgaatctc caccagtgat
     5641 tagtacacgg cggttttcta gttgattatt gccttcatag gattcttctc cacaatcagg
     5701 aacaggaatc atattctgct gtaaagctgg tgtatcctga tcttgtttag gaaatttctc
     5761 cgtatggtac aagtttcgcg ggtcgattaa ttttggttct gtcattgctg tattcctcct
     5821 agtttttaaa ttgtattgcc aaaacaaatt tctttgtttg atacctgttt taaatagatt
     5881 ctttaatttg tataatcttt ccggtataaa tggtaaccta aaactactgc tgctatcaaa
     5941 gagccaaaga ctacgagtat aaagcctaga gcagaacttt tacccggtaa tcctcctgta
     6001 ttcattcccc aaatccctgc gaacaacgcc ggaacggaaa tgattaatgc agctgaatct
     6061 agatatttca tgatatgatt tagattattg tccatcatcc cgttgaatag atcaccaata
     6121 ctctctaata aatctcgata gatctcgatc attttttctg tttgtcgctg tctcaaatgg
     6181 acatcataga gtagctgttc atcgtctaaa cgtttgatga aagtttcaga attccataat
     6241 tgatccattg tttcccgttg atctcttaag gtatgatcga tatatacgat gtctctttca
     6301 agatctgcct gtttatacag ttcttcattt tccgttgttt tacgagctgc tttatccaaa
     6361 gcgtcaattc gctttttcat ctctcttagt tctttcacgt aatgggtgta aatatctaat
     6421 aatgtatacg tgatgatctt ttcaaacgag tttaaatgat ttccatgctt tgtcagcagc
     6481 cgatccacaa attcggtttt tctatcgata caagtaatca agagattatc ggaaagtaca
     6541 aaagatattg gcgttaattt tttttcgata cgattatctg actgatctag attaatcact
     6601 accaaagaaa atggatgctc tagtttcgtc tcataaaggc gctccatccg agaaacttct
     6661 tccggatatt cggttcctac gaaaatatca tcaggaagct cgaattcttc gattgcttgc
     6721 tcaatctctt cgggctttgc actatcaagt acgagccaat tatatttttt ttcatctgtt
     6781 tctattacat tcttttcttc atccacaaag tagtatctca atgtatacct cctttttgga
     6841 ctttctcgaa actattagct gaagaagcag ttctttaatt tgatattata atcgagtact
     6901 gcgatttcat tttctaatga aaagctgtca agctgtttgt tgaagtccat gttcaatcgt
     6961 ttaccgaaat aattttgctc taatttagat gtatagatgt ccaataatgg atgctcgatt
     7021 ttagtcgcat attctccaaa ttccgaaaca aaaggaatga tttgaaaaaa gctgcctttt
     7081 gcaaaagcac ctttgtccct tagaaatgga tcggaatcat tgataagcgc gagtgcgatt
     7141 tttgtatttt ccccaacctt ttcaattaac gtctctactg catttttaga cattgtaatt
     7201 tccattccta ctccccctct tcagttgttg tcttctatta tttaacaact acatctgttt
     7261 ttggttgaac ttttgcatat tttggatatt tagggaattt agctacacaa cggaaagccg
     7321 tcatccctgc atatttttcg taatatttct tcgttggccc ttcaaaatcc ttgctccacg
     7381 gtttcacatg tcctgaaaaa tggataatgt aaggattgtt gcgagtttct tcgtattcac
     7441 gttcacctgc tgctgttgga tgtttttttg cttttgccat gatatagcct tgtgcattcc
     7501 aacgcggatg aagtggtaac caacggtcat gtaagatagc atttaatgca tcttggtcat
     7561 ggaatcgtaa tttttctgga ttatgttcga tgaaatctaa tactttttgc gtaatatttt
     7621 catctaacca ttttttcaca ttgatcagca tcaaacctga gttgaaataa cgcattgatt
     7681 tagctgaaat tttcattttt tccagtcgtt gatggaaccc agcgtcttca acagctgcta
     7741 cgattgaatc accgaagtca agacgccata atttagagat atcttgtaaa gcgatcatgt
     7801 cgcaatccat atataagata cgttcaactt ctgtgccgcg gaacaattcc ggaatcgcaa
     7861 tacgataata agctgtttct ggaatacgat cgctgattac cacatttgta aagaattctt
     7921 tattgatttt caagaattcc acatctgagt tcattcgtgc gtttttcact gagaagcgta
     7981 aaagctgttt actttcaaaa tcgatatcat cgtcaatcac atagaaccga acaaatttag
     8041 acgggttgca attatctaat actgagacaa ataatgcagc taaatgtggt acgaattttg
     8101 tgttacaact tgcgactact gctatttctt ttttattcat tgatctcgcc tactttcgtt
     8161 agagtactgt gtgctaattt ttttaagtat aaattggtat acggatgatc tttcaacgta
     8221 ttccaaggtt tatcatgtcc tgtaaagtga acgatcgaag gtttttcatt tccttcttta
     8281 tactgacgtt catatttttc atttggtgct ggatgacgtt caaaaatcag cgaagtctgc
     8341 atgttccatt ttggatgaag ttgttcccaa tcttcataaa gtacagcatt caaagcatct
     8401 tggtcgtgat agatgattcg atcgccattt tcactgaggt aatggatagt tttttctgta
     8461 atttcttttt tattccattg atcaatgtca atcaccatca ctcctgaatt gaagtacaag
     8521 tcatcggaat cgattcctaa tctttccaaa gctttcactt ggcctggatc gataatagcg
     8581 ccgattgttt ttccatttaa cggctcatca tatagtttta cgatatcgtc tagtactaac
     8641 acatctgaat ctaagtaaag gactttctta taatcctctt tagctaataa gtttggtaaa
     8701 gaaattctta agtaagcggt agttgtgata tggtcactga ctaggaagtc ttcgtagata
     8761 tctttttcca ctgtcaggaa ttggatactt gcattacttg aatatttatt taccgtttct
     8821 tccaatcctt ctttactata ttctgataga ccatcgtcaa tcacataaaa ttttatgcgt
     8881 cttgttttat tgcaattttc taatgcagtt gcgatcatca cgctcaagta aggagcataa
     8941 ttttcgtctg aagcagttac cacaggaact acgccgtatc tcttctccat caaaatcact
     9001 cctttttcta atctttttct aacctgttct acatctagaa tattcctcat ttggttttaa
     9061 agcaaaatat atgctttgtt ttcattgaat attttaaaat ttttttatca caaaacgcat
     9121 cggtaagcgc taactattcg tttttcatgg ctttttattc aaaggtattg accaaaaact
     9181 actatatata gccacctttt tcttaagaat gttttttgaa aaaattcata atgacaaagc
     9241 aagttagttt cattttttta aattctcatt taatcttaag gtaacaggaa aatagatatg
     9301 taaaaagaaa gtaggaagat aaaatgaaac tagcagcgat tgatttagac ggtaccttgc
     9361 ttgatcgaaa tggaaagata ccagaagaaa atatccaagc acttcggaaa tttgatgaaa
     9421 atggcggaat cgtaaccgtc gctaccggaa ggaacagcat ttctgctaaa gatatttttt
     9481 ccgaattagg aatcggtggc tatttgattt cttccaatgg ttcactgatt gctgaaatga
     9541 aggatggaaa aatggcgaaa gttctcagac gttcaaaaat agatagcgat acactgagaa
     9601 aagccttctt ctatgctcaa gaagcgaaaa tatccatcat tgctagccga gaaacccaag
     9661 atgatcagct gacattcgat gaatcaaatc tagtcaaaga tgacccttat tatgaacatt
     9721 tcaatttaca gaaccattct tttgatcaaa ttttaaatat gttggaagat ccttctctta
     9781 gctatcttaa attagcatta acagataaga gtgaagaaaa actacagaag ctacaaagag
     9841 atctgcaagc agatggtatc caatcagtat tctctgatcc gcatttctta gaaatcacgc
     9901 ctacaggtat cactaaagct cattcacttg tttttctggc ggattacctt ggaatcgaat
     9961 cgagtgacat catggctttt ggcgatcaag aaaacgacat tgctatgttg aattacagcg
    10021 ggttgagtat tgcgatgggg aatgcacagc gacaagtaaa agaaattgca aaccaagtaa
    10081 cactgacgaa tgaagaagct ggtgttgctt atattttaaa aaacttctat taaaaagaat
    10141 aaaatgacga tatgaatgta ggaggaaaga aaatgttagt tattattgat atgcaaaatc
    10201 atattcttga tccaaacagc gaattttata ttccagagtc agatcaattg gcagaacgta
    10261 ttgctcaaag attagctaaa gcacgccaaa ataatgaata tattctcttc acacgggata
    10321 taccagttga actgaaggac aaagaagaag aacgagaaga tttgcaattg atccctcttc
    10381 tttctccaca agaaaatgaa cgagtgatta aaaagtatta ctttacgatt ccacctgaaa
    10441 ctttagctga aatccaggaa actttatttg aaagcaaaga agaacaaaaa gagatcgaag
    10501 tagtcgggat tgaaacaaac ttgtgtgtac tctctaatct gataggcctg cagagtgctt
    10561 ttcctgaagc agactttttt gttgatccaa tgttagtctc cagtagaaaa cacggagaat
    10621 ccgctttaga gctgttgaaa gatttcaacg tctctatcgt cgaatcataa aaatatctat
    10681 cttcatttac gatatttaac acacaataaa aaaagcacgc agaactgttt tagtcctacg
    10741 tgctttttct atcaaagtag attggctttt ttatgacaca gagtgaaacg gcaggctcac
    10801 gacctctttc ttaaatggtg ttcaatcagc tgaccctcgg tattagctta acaaacggca
    10861 aaatatgagg aactattttt ccgtttgtta agctaatact caggtcaaaa cgctgatttc
    10921 acaacctctt ccttaaacgg tattcaaaag cttgcatctg cagtaataag aacagccaag
    10981 taaaaacata agaagctgtt tttacttggc tgttcttatt acttgatgca gggcagcttt
    11041 ttcacaacct cttatgtacc ttgtaggact cgaacctacg accggacggt tatgagccgt
    11101 ctgctctaac caactgagct aaaggtacat cataaataaa atttattttt gttacttttc
    11161 aactatagca cgaatgacaa actctcacaa gtttcaaaat ataagtaaaa taatattgtt
    11221 attcttattt cttttacttg tgtcataact attttgttac tttaattttt atttgcttat
    11281 tttagaaaaa atgttattct tttatttatt ctcaatgtta ggagtgatta ttttgccatt
    11341 tattgcgatt ttattcgatt tagttgcggc agcagcttat tttctccaat taaattatca
    11401 aacctcagga attctgttta ttggaatgat ttttcaaggt gtgattaccc ttttgcttct
    11461 tgtcatgatg ctcagctata aaggaaaaag atatgcccgt attcaaacaa aagtatttgt
    11521 taaatatgtt tccattcgct atgggatcat cattctttcc tttttagtga atgcgatcgt
    11581 cctcttttta tatgtattga attacttaga tatcaatcca ttgatttttt ctcgatagat
    11641 aagacacatt cagcggaaca gttcctgctt atcagcagaa aactgttctg ctttttcatt
    11701 tttcattgat ctattcttct tttattttcc tagcatttca tttgtgatgt atgcaataac
    11761 atgttatctt ttattatatg aatcgttgag gagtccgtta catgattacg ttggtgagag
    11821 ttttgttttg ggtgcctgcg gtcgcactgg tcgcaagtat tgtttatctt atgaattgga
    11881 acaaagaacg gttttattta gctattttga ctcttccggc tatctacttt atgtggaaag
    11941 tttttaatta caactacttt gaacccgata gtgtttttat agaagaatta tcaggactgg
    12001 ttttgtcttt attgatcgtc attctttatt tgatacgttt aaataaaaaa cattgagtac
    12061 tattgattgc atgtgtagct ttctatagtc attataacta ttctactata atgaaaggtg
    12121 attaaatgag tatcgaagaa gctattttac tggatacttt acgttgtcgt ctgaatttct
    12181 taagaactgg ccgatcttta tctcaagtag ctgtttctag cgcgttaagt attcctttat
    12241 tcaaatatcg taagtacgaa gaaggagaag aaattccaag catcattgaa ttaatctata
    12301 tcgctgactt ttacgatatt tccgttgatt acctaattgg tcgctcagaa acagcaagca
    12361 aactgaagtg attttttcca gccttggtgc tggttttttc atttaacgtc taaggttaac
    12421 aatctatata taaaggagac atttctatgc gattatggca tgaaaaattg atttcaaagc
    12481 ttcctcgtca acagctttta ggacaacatc gggaatgctg tgcattaaga ggaaatggct
    12541 ggggaaagaa acatgctact gtggattatg tttttcacta ttctccttat agattattcc
    12601 aatatcatca gcttgtcatg gatgaaatgg aaaaacgtgg ttacagccca gcaccagagt
    12661 ggaaaaatcc tatgtatcgt ggcaaatcat gcgaagcatt ttttgattta cctactgtag
    12721 agcgcaacgt gccgatttat ccggaacacg atgaagttta tctagcagaa tgtttagcaa
    12781 atttggagca aaaaggaatt tatcttacct aatataacta gaaaagcctt ctatctcctc
    12841 ttacttcatt aaaaagcttt aaccatctta gatcaagcta aaatgtcgta agttttaggg
    12901 ttatcttcat cctcacgtgc aaaactaaaa gcgtactcta gttttggttc atcgccaact
    12961 tccctagaca taaatgaaca tctcctgacg tggtttaaaa gtaccgcatc ttcgacgtct
    13021 gtatacgatg cacagcacag attgcttctt ttgatctttc ttttgatttt tctgttgctt
    13081 cttctacacc ttcttcgatt actacatcga tgcttacaat tgcttctgcc taatattccc
    13141 atgcatcgtc gatttaagcc tctgtagtca ctgtgatcgt ttccacattt taactcctct
    13201 gtcgtggctt cagttgattt tgtgatcagt tcagttgcta agccagagtc agaacaacct
    13261 gccattgcta gcgttaatat tgatagtaaa aatagttttt ttatcataag gttccccccc
    13321 tgaaaataga ctaaaataat aacgctttct ttttttgttt tcagacgaaa attaataatt
    13381 tctaaatatt ctttaaaaaa gcttataact
//
LOCUS       DAZABD010000071        13305 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000071 DAZABD010000000
VERSION     DAZABD010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13305
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.071"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..308
                     /locus_tag="ACHALG_002273"
     CDS             <1..308
                     /locus_tag="ACHALG_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN2587637.1"
                     /translation="HFARREKKLYRSLYLEEYGGGKEMQNFSYTYFVNTVKRDPAYVN
                     LSEEQIESLYNGTWIIATGIAALMSSSIIHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(486..926)
                     /locus_tag="ACHALG_002274"
     CDS             complement(486..926)
                     /locus_tag="ACHALG_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFN2587638.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLSGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            1084..1848
                     /gene="map"
                     /locus_tag="ACHALG_002275"
     CDS             1084..1848
                     /gene="map"
                     /locus_tag="ACHALG_002275"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFN2587639.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            1880..2803
                     /locus_tag="ACHALG_002276"
     CDS             1880..2803
                     /locus_tag="ACHALG_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFN2587640.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            2879..4018
                     /locus_tag="ACHALG_002277"
     CDS             2879..4018
                     /locus_tag="ACHALG_002277"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HFN2587641.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            4011..4811
                     /locus_tag="ACHALG_002278"
     CDS             4011..4811
                     /locus_tag="ACHALG_002278"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFN2587642.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            4811..5638
                     /locus_tag="ACHALG_002279"
     CDS             4811..5638
                     /locus_tag="ACHALG_002279"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFN2587643.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            5643..6350
                     /locus_tag="ACHALG_002280"
     CDS             5643..6350
                     /locus_tag="ACHALG_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFN2587644.1"
                     /translation="MRSVCIATFNGGRYLREQLDSILSQLSAEDEVVISDDGSVDDTW
                     DILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWLETKVKKITD
                     YFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYIGAGMAFRSS
                     LKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIETHSKLRQKIS
                     WRVHLITALIKRKWFKR"
     gene            6450..7316
                     /gene="rfbA"
                     /locus_tag="ACHALG_002281"
     CDS             6450..7316
                     /gene="rfbA"
                     /locus_tag="ACHALG_002281"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFN2587645.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            7330..7902
                     /gene="rfbC"
                     /locus_tag="ACHALG_002282"
     CDS             7330..7902
                     /gene="rfbC"
                     /locus_tag="ACHALG_002282"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFN2587646.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            7924..8952
                     /gene="rfbB"
                     /locus_tag="ACHALG_002283"
     CDS             7924..8952
                     /gene="rfbB"
                     /locus_tag="ACHALG_002283"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFN2587647.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            9050..9901
                     /gene="rfbD"
                     /locus_tag="ACHALG_002284"
     CDS             9050..9901
                     /gene="rfbD"
                     /locus_tag="ACHALG_002284"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFN2587648.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            9935..11968
                     /locus_tag="ACHALG_002285"
     CDS             9935..11968
                     /locus_tag="ACHALG_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587649.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPAKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKIDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKEIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            12012..13292
                     /locus_tag="ACHALG_002286"
     CDS             12012..13292
                     /locus_tag="ACHALG_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HFN2587650.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVCTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIATLLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
BASE COUNT         4083 a         2251 c         2821 g         4150 t
ORIGIN      
        1 tccattttgc aagaagagag aagaagttgt atcgttcact gtacttagaa gaatatggtg
       61 gaggaaaaga gatgcagaat ttctcttata cttactttgt gaatacagtg aaacgcgatc
      121 cagcttatgt gaatctttca gaagagcaga tcgaatcttt gtataatggg acatggatca
      181 tagctactgg gatcgcagct ttgatgtctt caagcatcat ccatccaact gatgaacaga
      241 ttgaaaagat gatacaagac agtatagatg ctatcttgaa attcaaagaa ccaatagaaa
      301 tcgattgaat atactcaaaa gttccacaaa tcagtagata acaagagatg tgtcaaaata
      361 aggaaaagtt cgtttttctt acagcgtatg catttttaac tcccgaagaa ctcttccatg
      421 aatgcaatga ataaaagagc cgtacagatt gaataaatct ggatggctct tttattgcaa
      481 gtatattaaa tttttgcgac tagttcttta gcgaaagctt ctaaattttc gatatcttct
      541 tcttcagcat tcagatccac tttaacattt tcggctcctt ttgatgcgcc tgtttttgca
      601 aaagcagcat cgaaatcatc caccgatttg cagaattcat cgtaaaaagt atcaccagat
      661 ccgcagacac cgtaaatttt tcccgataga tccaattctt gcaagtcttc ataaaagtct
      721 acgatctcat caggcagatc gccatcatcg tatgtataag tagctactac gcaaatatct
      781 gcctcctcga attcatctgc atctacttgg gtacattcgt tgatttcgac ttcgatatcc
      841 agattttcaa atgcttctgc aacaatatcg gcgatttctt cagtgtttcc tgtcatgcta
      901 gcatatacga tttttgctaa agccatgatg tgcctcctcg tttataatta tttaacaatt
      961 atgaattata ccaaaacatc attgaaaaga aaataaaacc tagagacacc tggggaatgt
     1021 catttggcta aatgtatgcc gctatgttaa aatgagaggg atttgtaaaa aggagacgaa
     1081 aatatgatta ctttaaaatc agcacgtgaa attgaaatga tggcagaatc tggagcttta
     1141 ttagcagatg tacataaaaa tctgcgtgat tttataaaac caggaatcac aagttgggat
     1201 attgaggtat ttgtcagaaa ctatatcgag agtcatggcg gaattgctgc gcaaattggc
     1261 tttgaagggt atgaatatgc gacttgtatc agtataaatg atgaaatttg tcatggattc
     1321 cctcgtaaaa agcctttgaa aaacggtgat ttggtaaaag tcgatatgtg tatcgatttg
     1381 aaaggcggca tttctgattc ttgttgggcc tatgtagttg gagaatctac acctgagatc
     1441 gaccgattga tggaagtaac gaagaaagct ttgtacatcg gtattgagca agcacaagta
     1501 gggaatcgga ttggtgacat cggtcatgct atccaaactt atgtagaagg cgaaaatcta
     1561 gcgatcgttc gtgattttat tggtcatggc gttggtccta cgattcatga agaaccagca
     1621 gttcctcatt acggagaagc tggaaaaggg ttgcggttga aagaaggcat ggttatcacg
     1681 atcgaaccta tggttaatac cggaacttgg aaaatgaaaa tggatcctaa cggctggact
     1741 gcgtatacaa gagacggcgg actttcttgc caatatgaac atacattggc tattactaaa
     1801 gatggtccaa gaattttgac atcacaagga gaagaaggca cttattgatc tatccagtca
     1861 ataggtacgg gagaggtaca tgaaagtttg gaacaaaata aaacaaaatg aaaatctgat
     1921 gcgctttatt gagacgactc aaaagcgcat cactgattca gaaatgagta cgacctctgt
     1981 cgtcgttgct tattatttac tcttgtcttt atttccattg attattgctt ttggtaatat
     2041 tttaccgttt ctccacatcg atcaagagac tgtactgacc tatttacgag aagtgattcc
     2101 tgagaccgtt taccagttta tcggtcctgc aatcaaagac cttttgacac aaagttcagg
     2161 aggcttgtta tccgtttctg cattagcggc tttatggtct gcaagtcaaa gtatcaatgc
     2221 tttgcaaatt gcgatgaata aagcgtatgg cgtagaaaat agaaaaaatt ttttgatcgt
     2281 ccgtttcttc tcactaattg tcattgtctt gttcatgatt gctattagtg gggtaactat
     2341 cgttcttggg ttgggacagc tgattttgga tgctattcag ccaatcgtac aaatacccgt
     2401 gaactttatc gatcagttcc aaactttgaa gtggccaatc acattggtag ctttatttgt
     2461 catcatgttc ttgatttatt taattgtccc taatgctcag ttgaagttga agacgatcat
     2521 tccgggcacg atcttcgcta ctgtcggctg gatgctgtta tcacaagtgt ttagtatcta
     2581 tgctaggtat tttgcaacga ggatcagcgg ttatcagatc ataggaagtt ttattgtact
     2641 catgctttgg cttaattttg ctgctacgat catcattttg ggcggaatca tcaatgcagt
     2701 tgttcaagaa tacgtgacgg gtcatcaaat caaaaaacgt cgagatccaa gccgaaaatg
     2761 gttcaataaa ttgaaaacta aattttctca taaaaaaaag tagaaacaag agttcttatt
     2821 gtaaatttat atcagtaatt tataataatt atgactctta attaaagtag gtgcccatat
     2881 gcttgtttct tttagtatgg ttataagatt ttttctgaca attttattat cactgatatt
     2941 aacacctgta ttcaaaatca tttcagtaca aacaggtatg gtcgacaaac caaatgaaag
     3001 aagaataaat aaagttccaa tgccttcagc gggcggactg cctatcttta tttcatttgt
     3061 tatatcgact ttattcttat tccgtaaaat tatccctcaa tcgtatattg taccgatctt
     3121 gattgcttct ttagtaatca tagtcacagg agtgctcgat gataaatatg agctgacacc
     3181 taaacaaaaa agtataggga tcttgattgc ttctcttatt atttattttg tagcagatat
     3241 acgtattgat tccttcacgc ttccttttat tggatatatc caactaggct ggcttagttt
     3301 tcccgtaacg attttttgga tcttcggtat cacgaatgcg gtcaatctga ttgacggttt
     3361 ggatgggctg gcggcaggga tttcattgat cggtttaatt acgataggaa tcattggata
     3421 ttttttcctc catgcttcta cagtgtatat tccagttgtt attttctgtc ttgtagcaag
     3481 tattatcggt ttttttcctt acaattttta tccggcaaag atttatttag gcgatacagg
     3541 agctctattc ctagggttta tgatggccgt cctttcacta caagggctga agaacgtgac
     3601 ttttgtttca tcgatttctt tgctgatcgt catgggagtg ccggtaacag acacgttttt
     3661 tgctatcatt aggagaaaag caaaccgtgt ttctttttct acagcagata aaaaacatct
     3721 tcatcaccga ctaatcagtt taggctttac tcataaaggt gctgtcctga cgatttatgc
     3781 gatggctttg atgttttctt tcacagctat ggtcatgaat tatactggaa gaatcggaac
     3841 gatcatattg attattgcca tgttgtttgc tgcaatttta ttatttgaat tgattggtct
     3901 gatcaatgag aaacatcagt ttattttaaa aacattgcga tttttaggga ataaagagta
     3961 ccgtacgcaa gtaaaacaga aatatttgaa gaaatggaac cgtcatatag atgaataaca
     4021 aattaaacca gccgatcctt attagtattg tgacggcaaa cagtgataaa atttttcgga
     4081 cactagataa ttttatttca actgtaaaag atgaaacagc agtaaaaatc cgaatttttg
     4141 acaacaattc acttcccgag tacaaaaaaa gactaacaga gtataggaaa tatccctaca
     4201 tcgatatcta ttttcatgat gaaaattccg gatttggtta tggacataat cacaatctga
     4261 tagctagcga ttctcgctat ggcattattt gtaacccaga tattttagtg gatgaagaga
     4321 cagttacagc attggtcttg ttattgaaag aacatccgga atgtgcgatg ttagcaccta
     4381 agattcttaa tgaagatgga acaactcagc acctcatccg tgagcagctg gctgtttttg
     4441 attacgtttt gcgctttgtg cctttcaaat gggtcaaaaa aaagtttgac aaacgactgg
     4501 ctgtgtttga atgccgagga ttgcctactg atgctaatag ttatgtacgg atgatctcag
     4561 gtagttttat ggtagctgat attgataaat ttaaagagat aaaggggttt gatgagcgtt
     4621 tcttcatgta ctttgaagac aacgacttgt gtttgagttt tgaaaaagca ggttacaagt
     4681 tattgtacac acccttttat gcagttatcc atttttacgg gaagggtgcg catcgaaatg
     4741 caaaactgtt tcgcgtgttt ttgacttcta tgatgaaatt tttcaataag tgggggtggc
     4801 gttttttctg atgaaaccag aaatattagt agtcgttgtg ctttatcagc aactattcag
     4861 tcaatctcca gcttatggac cattaagcaa agcacttagc gaaaagcaaa ttcagcttat
     4921 tatttacgat aatagtcctc aaaggcagca aaatgagctg tttgaaaaag aaaatatcgt
     4981 ttatcatcat gatccgcaaa atcctggttt agcggtggct tataattttg cgttgtctca
     5041 agcagatgaa ggaacacgtt gtctcgtcac tctggatcag gatacgaggc tagtcgacga
     5101 ttattttgaa ctattgcgta aagtaacctt tacagatgaa tgtgttgctg cagttccgat
     5161 gattttttct gggagccgac aagtttctcc cctatattca gatcgttata tcaatcgtac
     5221 agctcaagca gtagaagttg aaggcccaac agctgaacga atcatggcaa tcaattcagg
     5281 tgttgcctgg tctgtcaaat ttctaaaaaa gattggcggg tttaatcctg ctttttcttt
     5341 agattttttg gatcattggc tgttttggaa agtggatcag ttggaaaaaa ctgtggagat
     5401 ccttcctgta cgaatggaac atgatctttc ggttttagac tatgaaaaag tttcaagtaa
     5461 tcgataccgt tctattttac aagcagaaaa tcgattttat catgaatatg atcaaaggca
     5521 tttatccagt caccgacgac aattattgtt aagaacagct aaacagttct tgaccgtcaa
     5581 aaaccggcat atctggcgga tgactttgcg ctcatatgtt aagaattgga aggtgtgaat
     5641 tcgtgcgttc ggtttgtata gctaccttta atggaggcag atatttaaga gagcagttag
     5701 atagtatttt atctcagctt tcagcagaag atgaagtagt catttctgat gatgggtcag
     5761 tagatgatac atgggatata ttaactgaat acgcagaaaa agataagcga atccagcttt
     5821 tttcggggcc tcgtcaagga ttgatcgcta attttggtta tgcgatccag cagacgaaag
     5881 gagatctaat ctttttggca gaccaagatg atgtttggtt ggagacaaaa gtgaaaaaga
     5941 tcactgacta ttttgagcag aatccggatc agctagtagt agtttctgat ttaatcattg
     6001 tggacagcga gctgcaagag atccatgatt cgtatttttc gtttcgaaat tccaaagaag
     6061 gatggcttaa taatgtgata agaagtcact atattggtgc tggtatggct ttccggtctt
     6121 ctttaaaacc cgatattttg ccgatcccgt cagaagtgcc gatgcatgat atgtggattg
     6181 gtttgctggg aggaaagaag actgggttcc taagagaacc gttaactctg taccgaagac
     6241 atgatttaaa cgcgagtgag attgaaacac attctaaatt acggcaaaaa atttcttggc
     6301 gtgttcatct gattacggcg ttgatcaaac gaaaatggtt taaacgttga taaaagatgt
     6361 ttaaatcctt aatatttcca tgacaaatat aacaattgat gagataataa caaagtgaaa
     6421 tttgaaatca aaaaggagag cagatatcca tgaagggaat cattcttgct gggggcagcg
     6481 gaacacgtct gtacccttta acaaaggcaa catcgaaaca attgatgccg atttatgaca
     6541 aaccaatgat ttattatcca atgtctactc tgatgttggc gggaatcaaa gaaattctga
     6601 ttatttcaac gccacaagat acaccacgtt tcaaggaatt atttggaaac ggaaatgatt
     6661 taggactgca gatcgaatat gcagtacaag aaagtccaga tggattggca caagcgttta
     6721 tcattggaga agaatttatt ggagatgata gtgtctgctt ggtattaggg gacaatattt
     6781 actatggtgg cggtctgtct aaaatgttgc aacgagcagc ttcaaaagat acaggcgcga
     6841 cagtatttgg ctatcatgtc aatgatccag aacgctttgg tgtagtagaa tttgatgaga
     6901 atatgcaagc tttgtcgatt gaagaaaagc ctgctcagcc aaaatcaaat tacgctgtaa
     6961 ctggccttta tttttatgac aatgaggtaa tttctatcgc taaaggtatc aaaccttcag
     7021 aacgtggaga actagagatt acggatgtca ataaagcata tttggataaa ggaaaactat
     7081 ccgtagaact tatgggacgc ggatttgctt ggcttgatac aggaacacat gaatcattgc
     7141 ttgaagcatc tacctttatc gaaacaattg aaaaacgtca gaacttaaaa gtggcttgtt
     7201 tggaagaaat tgcttaccgt atgggctaca tcgataagga acaattgctg gcccttgcac
     7261 agccattgaa aaaaaatcaa tacggtcaat atttattgaa tttggccgct gaataaggag
     7321 tccttacaga tgaaagtaac agatacaaaa ttaacagatg tcaaaatcgt agaaatggac
     7381 gtctttggcg atcatcgcgg tttcttcaca gaaagttatt caaaagcaaa atttgcagaa
     7441 catggattga aatatgactt tgttcaagac aaccattcac tatcagctga agctggtgtc
     7501 cttcgggggc ttcatttcca aaaaggagaa gctgcacaga caaaattgat tcgggtagtc
     7561 actggagcag ttttagatgt agtagtggat atccgcaaag gtagcccgac ttatggtcaa
     7621 tgggacggat ttatcttatc tgaacataat catcgacagt tgcttgtacc taaaggatat
     7681 gcccatggat tcgttacttt gacaccaaat gtgaacttcc tttataagtg tgataattac
     7741 tataatgcag aagcagatgg gggcatcgca tttgatgatc cagatttagc gattgattgg
     7801 ccaattgatc catcaagagc gattatgtcg gaaaaagatc accgtcatcc gacattaaaa
     7861 gaatttgaag cagaaaatcc atttgtttac ggagaaatct aagaattaag gagaaatttt
     7921 cttatgaaaa acatcattgt taccggaggt gccggattca tcggctctaa tttcgttcat
     7981 tacgtggtaa acaatcatcc agaagtgcat gtcactgttt tggataaact gacttatgca
     8041 ggaaacaaag agaacttggc tggacttcct agcgatcgcg tagaattggt cgtaggagat
     8101 gttgcagatg cagaattagt ggaccgtcta gtaaaagaag cggacgcagt tgttcattac
     8161 gcggcagaat cccataacga taattcatta aaagatcctt tcccatttgt acaaacgaat
     8221 atcattggta cgtatacact tttagaagct tgccgcaagt atgatgtacg ctatcatcac
     8281 gtatctactg acgaggttta cggcgatctt ccattacgtg aagacctgcc aggacatggg
     8341 gaaggcgaag gagaaaaatt cactgcggaa acaccatata atccttcaag tccatattct
     8401 tctacaaaag ctggttcaga tctgttagta aaagcatggg tgcgttcttt tggcttacgt
     8461 gcaaccattt caaactgttc aaacaactat ggtccttatc agcatattga aaaatttatt
     8521 cctcgccaag taacgaatat cttaagcggt attcgtccaa aattatatgg cgaaggaaag
     8581 aatgttcgtg actggatcca tacgaatgat cattcttcag cagtgtggct gattttgacg
     8641 aaaggcagaa ttggcgaaac ctatctgatc ggtgcagacg gtgaagaaga taataagacc
     8701 gtgatggaat tgatcttaga aatgatgggt caaccaaaag atgcgtacga ccatgtaaac
     8761 gaccgtgcag gacacgactt gcgttatgca atcgattcga caaaattacg ggaagagtta
     8821 ggatggaagc ctgagtttac gaacttccgt gatgggttgg ctgacacgat caaatggtac
     8881 gaagaacatg aagactggtg gaaaaaagaa aaagaggccg ttgaagcagc atatgccaaa
     8941 aatggacaat aatacagaga aaatgtcact gtattaatgg aaaaacacat gtgaggcagg
     9001 cgctcgcctg tctctttttt aaaataaaaa tcaaggagcg gataatcata tgtttttatt
     9061 aactggagga aatgggcaac taggtacaga attgcgccat ttattagatg aaaaaaatct
     9121 gcaatatgtt tcaacagatg caaaagactt ggatatcaca gatgccgaga aaacaatggc
     9181 ttacatcaca gaattaaaac ctgatgttat tttccattgt gcagcttaca cagcagtaga
     9241 taaagcagaa gaagaagcaa aagaattaga tgagaagatc aatgttgatg gtacaagaaa
     9301 tgtagcactt gcagcaaaag ctgtgaatgc tcaattcgtc tatatcagta cagactatgt
     9361 tttcgatgga aaaaacaaaa atgtagaata cgaagtagac gaccaaacga atccactaaa
     9421 tgaatatggt cgtacgaaat tattaggcga aaaagcagta gaagagattt tagaagacta
     9481 ttatatcata cgaacttctt gggttttcgg gatctatgga cacaatttcg tcttcacgat
     9541 gcaacgccta gcggaaaccc gcgacaaact gactgtagta aatgaccagt ttgggcgacc
     9601 aacttggaca cgtactttgg cagaatttat ggtatatgtc atcgaagaaa aagcaccatt
     9661 tggtatttat catttgtcga acgaaaatag ctgttcttgg tatgaattcg caaaagaaat
     9721 attgaaagat acagatgtag aagttgcacc tgttacgtca gaagaatttc cgcaaaaagc
     9781 aacacgtccg cagtattcag tcatgagcct gaaaaagaca gaagagttag gatttgtcat
     9841 accaacttgg caagaagcac ttgcgcaaat gctggaagct gccaaaaaaa tgaataagta
     9901 atttcctttt atcaaataga atttggagca tgatatggaa aaacaaacaa gaagtgcacg
     9961 acgaaaagaa aagccaaaag cgaaccaggc agattcgaat ttggtgcttg acctgtttct
    10021 aaaactccgc tatcttattg gcgttttagt catcgtggtc atcgttactt ttaatttaaa
    10081 cggaagttca cttggtgttt gggataaata tgtgtcgcaa agagacgatg ggaaaaaaac
    10141 tgatgtgatt tttggacaga atcgggaagt gcggtcagac gaatggctgg tgcagacacc
    10201 tttttacctc tcacaagctg aagaggactt tccgttggtg aacgaggatt attcattaaa
    10261 cggacaaaac atgatcattg cgtataattc tcctgcaaag gatattacgg ttatcggaaa
    10321 accattcaac tggggattct ttttcctcgg aagagatcga ggactttcct tttattgggc
    10381 gatgaaatta gtcgtcatgg tcttgcttgg attcgaagta ctgatgatat taacgaagag
    10441 aaaaaaagct ctatcgctaa tcggtgcatt tgcgctgaca ttttctccag cagtccagtg
    10501 gtggttcatg caacatgtgg gcgatttgat tttctttact ttgggactga tgactgcttt
    10561 ttatcattat ttctacaatc atgataaaaa atggctacga acgttgatga tgcttttggt
    10621 ggttattttc ggtattgggt ttattttagt gatttaccca gcccatcaag tgatgctggc
    10681 gtacttgctg attttttatt ttgttggcct gcttatttac tatagcaaga aagtttcatg
    10741 ggatctgttg gatgtagtgt tgattggagg ggcagtttta tttattggcg gagtcatggt
    10801 tcatttctgg ctaacgtcaa aagacgcatt gttagcttct ttgaatacct tatatccagg
    10861 aaaacgggta agtactggag gaaagtggac gattggcgaa tttttttctt tccttacaaa
    10921 ttggaaaatt ccttttgaag atatcaacta ttcgaataac tctgaagtgg ccctgttcta
    10981 tcactttttt ccaactgtat tcctagcaag tccatttgtg ttatttggca aaaaagatag
    11041 cgaacaaaag ttactgggac gtattttgat gattttttgt ctgttttcta tcttttggat
    11101 taccgttggg ttgccaaaat ggctagcaga aatcactctg ctgtcttatg ttcctccatt
    11161 aagagccatc cttactttta gttttgcggc ttgtttatta tcgatctggt ttatcgctta
    11221 tatttggaga gaagatgtta ttcctatttg gtataaattc attttgttgg cagtaaatgc
    11281 atggtcttat ttctatgcga ttacgcattc gaaaatagat aattattttt cggattttga
    11341 aaccatcgca gttgctgtgt tgggaacagt gattttagca tttgcattat ttggctggag
    11401 acgcttgttt tacctcgctt ttgccgcctt agttatcgta tctggctttt tcgtcaatcc
    11461 agtcgtagaa ggtacaggag cgatttttga aaaaacactg gcaaaagaaa tccaaaaaat
    11521 cgatcaacaa gatcctggta aagtttggtt gtctgaagat gaattatata acttcacccc
    11581 aacattggga gtgaaatcct tcaatacagt acgcttttat ccagatatag ctgcatggaa
    11641 ggaaatcgat ccgaaagaag aatacgagaa gtattataat cggtatgctc atacaagagc
    11701 ttttatcgct aaggaagata caaagttcat tttagatcgt ccagatatgt tttcggtcac
    11761 gttgaatttt gaagacgcag aaaaattagg gatcgactat gtgatcagta aacgaccatt
    11821 aactgattac aaccaattat atgaagcgca gttcacgcag ctatatggac cggataaaga
    11881 cggttatatg atttttaaag tagaatatcc ggattatcca gatgtaccac aaacacaaac
    11941 tcaggaaaat cagctagtcg tacagtaata ggctagctga gaataatcct gaaatattag
    12001 tagaaaggag agtggagata attcaagcaa tcgtacaaaa atttgtcaac aaaatgagtg
    12061 tcggtttctt gcttttgttg ctgacgctag tcttttttgt ttgtacagca gggttaggtt
    12121 ttccagcaac tttgtttata tataacaata gttcattact tatcgctttt agtgtggcta
    12181 ttttgctcat tttaaatgtt tataaagcaa aaggcgtcga ttttctttat ctaggtgttg
    12241 cgcttgccct tttcattttc ttttcttact ttcgggaatt aagaaatgag tctttatttg
    12301 gagatttact gtttcctttg attattttat tttgtttagt aattaaatgg atcgagtgga
    12361 caaaatggga tcgcggtatt tatctgattg tctttacggt cttcctcgga atcacgttat
    12421 atcgtgtatt tacagagatc caagtaccag aaggacaaag catctgggct ccaggcaatt
    12481 cgctttctga tatttggatc aatacgaata caatcggaag ttctttgatg actcttgctt
    12541 tattgatcag tggatttgcc agttcttttg aacgatggta tgtacgaatc atcggtgcag
    12601 tagcagttgt tgcagctttt ctcgggattt gggtctgcca atctagaggt gcattagtcg
    12661 cgttgattgt ttttgcgata ttggatcttt tacctaaatc gttgatgcgt gtcattcgtg
    12721 cgccatttat tgcttacacg gttacgattc ttttagcttt gccgatttct tatctggcgg
    12781 ctgtctctga aaaagtcaat ttgtttaccg gtcgagaaga tatttggcat aaattctatc
    12841 agacgctagg agaaaaatct gaacaagttt tactaggaat gaaaacattt atcttccaac
    12901 gaggaaacca atttttagga aaccacaata gttataattc tattttgaat atctatggat
    12961 tgataggatt tgggattgct actttgctgc ttatcctttt tattggtcgc ttaacgttga
    13021 aagcagattt atccaacggg cagatgactt ttatctgggc cttttttgcg gtgatgatgc
    13081 agtcatttat ggaggataca ttgacttcat acccatgggt tccgattatt tatatcctgt
    13141 tggctatggc cgttcatcga tatgatgaac cagaagttgc ggaggaattc tatgttatgg
    13201 aagaggcata tgacgatatg acttatgatg atacatctta tgaggaacaa ccagatagcg
    13261 ctcattcttc caggatccaa cgctatcatt gataggcaca aaata
//
LOCUS       DAZABD010000072        13215 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000072 DAZABD010000000
VERSION     DAZABD010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.072"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            113..853
                     /locus_tag="ACHALG_002287"
     CDS             113..853
                     /locus_tag="ACHALG_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587651.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(1144..1812)
                     /locus_tag="ACHALG_002288"
     CDS             complement(1144..1812)
                     /locus_tag="ACHALG_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFN2587652.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            1946..2233
                     /locus_tag="ACHALG_002289"
     CDS             1946..2233
                     /locus_tag="ACHALG_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587653.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            2649..4187
                     /locus_tag="ACHALG_002290"
     CDS             2649..4187
                     /locus_tag="ACHALG_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HFN2587654.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            4184..5149
                     /locus_tag="ACHALG_002291"
     CDS             4184..5149
                     /locus_tag="ACHALG_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN2587655.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            5136..6458
                     /locus_tag="ACHALG_002292"
     CDS             5136..6458
                     /locus_tag="ACHALG_002292"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFN2587656.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      6521..6625
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            6699..7442
                     /locus_tag="ACHALG_002293"
     CDS             6699..7442
                     /locus_tag="ACHALG_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN2587657.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            7457..8449
                     /locus_tag="ACHALG_002294"
     CDS             7457..8449
                     /locus_tag="ACHALG_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN2587658.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            8461..9198
                     /locus_tag="ACHALG_002295"
     CDS             8461..9198
                     /locus_tag="ACHALG_002295"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN2587659.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            9188..9910
                     /locus_tag="ACHALG_002296"
     CDS             9188..9910
                     /locus_tag="ACHALG_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFN2587660.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(9994..10617)
                     /locus_tag="ACHALG_002297"
     CDS             complement(9994..10617)
                     /locus_tag="ACHALG_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFN2587661.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQP
                     VVQETVQTEKQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYI
                     GRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            11012..11704
                     /locus_tag="ACHALG_002298"
     CDS             11012..11704
                     /locus_tag="ACHALG_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFN2587662.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
     gene            11912..12202
                     /locus_tag="ACHALG_002299"
     CDS             11912..12202
                     /locus_tag="ACHALG_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587663.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(12474..12823)
                     /locus_tag="ACHALG_002300"
                     /pseudo
     CDS             complement(12474..12823)
                     /locus_tag="ACHALG_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            12916..>13215
                     /locus_tag="ACHALG_002301"
     CDS             12916..>13215
                     /locus_tag="ACHALG_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HFN2587664.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKY"
BASE COUNT         4508 a         2191 c         2693 g         3823 t
ORIGIN      
        1 atatttatga aattcatcgc tattttctta aaataatcag cgcattgcta ctgaaaacaa
       61 tgaatctgtg ctattattga gaaaagttcc atttttgaga aggatggttt gaatgaaaga
      121 aatcagacga aaacatttcc gttttcctgc gtacgatgat gagttaggcg taaaattaac
      181 caacgacaag aagcgagtat tatttgacgg acaagaagat cttttgactg aacagaatca
      241 gtccccgttt gaacagatga aagaatttca tttcacgaac acgaacaccg agaaaatccg
      301 tcgcaagcgt cgtgcaacaa atggattgaa gtcagctccg cgtaaggaaa atttggaaaa
      361 gcataaagga aaattaccgg attataccgg ccattaccaa tcagaaatca catcgacagg
      421 acgaaaaaaa ctttttggtg attcctctcg ttctgtcaga caaagtgaaa caaggacaga
      481 gtccaagcat actacacgaa acgcatctgt tgacacgaag gtgcgggagc gttcttattt
      541 tgttcctaaa tatgtacccg catcgattat cccagaccct agagaaagca aaatttctga
      601 ggatcattta atcgaatcga tgaaaaaaac ggatgactct tatctgttgt tcgatacaga
      661 accgacgccg tatcaagaaa aaagaaatgg tgagccgtct gtacgaacat tcaatcgaac
      721 aaaaacgtta gaggaaacaa agcagtcaga agaaaaaaaa tcgaaaaaaa gccatggtat
      781 cttagaacgc tcattacaag gattgatcga tgatcaagga aactctttgg gagaaaatag
      841 ctattttaaa taagtgatat acaaatttat attcggctac agttggaaag gaaacgctgc
      901 ggcgtttcct tattttatac tctatccgaa agtttatatc gtatcctcaa atttttttga
      961 gtttaagcga attttttctc ccaataagta agaaaataat ggcaaaataa aaaaacaaac
     1021 tattttcgca gatcgtttgc aaaaatagtt tattttttta taaagaagat tttactgaat
     1081 aatatcattt tagtttacat aatgatattt ctgtaaatta aataaagtag tgaaaaataa
     1141 gaatcagtgc tctaactgtt caagaggatc aagtggttca aatgtttctg tagtctgttt
     1201 tgcttccaat tggggcaaaa cagatccaat ttccagatac ttcactttat tcaatgtata
     1261 agatagaata gaacagctaa gtaatgtata cagaaaatgt gtgagatcca aataaagcaa
     1321 ggagacaact aaaataccaa aagtaatcaa gaaaagactt ttgccaatct taatatgaaa
     1381 atatttttca atggcttgat aaatgatatc tgttccgcct gtgcttccgc cgaatcgaaa
     1441 gacgagaccg caaccaatgc cagaaagaat acctgtcatc aaactattga tcagtagact
     1501 atctacagca aaatgtatat gaaggctctc ccataaatgt aaaaagaaaa ctagcgcaaa
     1561 tgttccgacg atcgtccgga gaaaagcgtg ttttccaaaa agaaacaatg agataagcaa
     1621 taaaggtaaa ttaaaaagca gtgaagtgac agaaggagcg aatccaaaaa gattatgcaa
     1681 aagcaaagca ataccagtaa ccccaccatc tgccagctta gatggaatat tgattcctgt
     1741 cacagctaat ccgtagattc ctgcgcctaa aatgattccg cacaattctg taaataaatt
     1801 taattttttc atttctagcc tcttttcttt tgaaaatttt cagcaacttc tttattatac
     1861 tgacttatac tcttacttca agaggaaaat gaaaaaaaat tatttttggc tgtttctttt
     1921 tatttatcaa ataaggagtg gaaaaatgcg agcagcagac ttattgacaa ttcttacaaa
     1981 atcacccaaa aaaacagagt atcaaggagt ttttttggct caagaacaaa aactggtgcc
     2041 tgtgacaatg gtccagataa ataaagcaaa tcaatttatc tttttatttg aacacggcaa
     2101 agcaccactt gctatgaaag acatctttct ttttttgatg aagaaccgtg agaaagctgt
     2161 ttattatcag tcaaaagaag aactcattcc tctttatgga gtgaaagaga tagaaaataa
     2221 agtggtgata taagtaaaaa taagaaaaca agacgtttat gatgtcaaaa caaccatttt
     2281 ttacattttt ggcaaaaaag tgtcatgata cagtataata ttcgttgaac aggccaataa
     2341 aggtgttctc ccccacgaga tttctacttt attggctgtt tctttttatt aaaataaaag
     2401 ttgtgtgtgt aaaaaagata tagctgattc caagtgacaa tttcgtatag atttttatgg
     2461 tattcgtagt gaaaaaacga gaaccaaaaa gttgatccga ctttgttgct tggattcttt
     2521 ttttgtcagc atatttagag gtattttttt cttttttttg ttcgtgctgt aaggttaagg
     2581 caagacaagt tagttgggaa aaaggaaaga aatgtttata cttaaagaat aacagaaagg
     2641 aaggtcgcat gaataatttt atacaaacat taagtgaaag aaaaggcgag ttgctggaag
     2701 ctgcatttca acatctgtcc atttctctca tttctttgct gattgcagca ctgattgcta
     2761 ttcctttagc gatttgggca gccaatcata aaaaaatggc agaagtctta ttgcagatca
     2821 cgggtgtgct ccaaacgatt ccttcacttg ccttattagg tttacttatc ccatttgtag
     2881 ggatcggaac tgttccagca ttgattgcac ttgttattta cgctttgctt ccgattttcc
     2941 aaaacacgta tattggtcta gctgaaattg acccgtctat tgaagaagca gcggtagcat
     3001 ttggtatgtc cagaatgaga cgcttgctga aagtagagct tcccatcgca ttacctgtca
     3061 ttatctcagg tatccgaact gccttagttt taatcatcgg tacggctact ttggcagcat
     3121 tgatcggagc aggtggatta gggacattta ttttgttagg tattgaccga aatacacctg
     3181 ttttgacatt gattggtgcg atcagttctg ccttgcttgc aattatattc agcggactga
     3241 ttcgctggtt gcagcataag aaacctcgat attccatcat tactttagca gcagtggtgt
     3301 gtttatttgg cggcgtttct ttatatcagt ctgccttgtt taaaaatgaa accatcacga
     3361 ttgctggaaa attaggagcg gaacctgaaa tcctgatcca aatgtataaa gaattgattg
     3421 aagacaacac aaaaacagaa gttgaattaa aaccgaattt tggcaagacg agttttttgt
     3481 tcaatgcact ggatcaaggt caaatcgata tctatcctga gttcacaggt actgtcctag
     3541 aaagcttggt gaaggtacct gaagcaatga aaagtgctga tttgacaaaa gaaaaaacat
     3601 atgacgaagc agaagaatta ttgaataagc aattcgatat gacattatta aaaccaatgg
     3661 cttaccaaaa tacgtatgcc ttggcagtca agagatcttt tgcgaacgaa catcaactta
     3721 aaaagatttc cgatttggca gctatctctg atcagatcaa agcgggattc acactggaat
     3781 ttattgatcg ttcagatgga tataaaggaa tccaagaagc atatggcttg aatttttcaa
     3841 gtgtacaaag catggagcca agtttgcgtt accaagcaat caataatgga gatgtcaata
     3901 ttgtggatgc ctattccaca gatagccagc tcaaggagta cgatctagtg actctagaag
     3961 atgatcgagg gttattccca gcttatcaag gcgcaccgct gatgaaaaaa gattttgcta
     4021 aagaacatcc tgaaatcgtt aaagcactga ataaattaag cggaaaaata acagaggatc
     4081 aaatgattga tatgaattac caagtcaatg tcgaaaagaa aaagccgaca gaagtagcac
     4141 gaatcttcct gaaaaatgaa ggattagtaa aggaggaaga tcagtgacta aattaatcga
     4201 gttcaaacat gtgcaaaaaa agtatgatgg aaagtatgtc atcgatgacc taaatctggc
     4261 aatcaaaaaa ggagaaatct ttgttttggt tggtccttcc ggtagtggaa aaacaactac
     4321 tttgaaaatg atcaatggat tatctaaacc atcagcagga gacatctact tcaaaggaaa
     4381 atcgttaaat gaatataatc tgcagaaaat gcggtggaat atgggatatg tgctgcagca
     4441 aatcgctttg ttcccgacca tgacagtcaa gcaaaatatt gaagtcatcc ctgaaatgtt
     4501 aggttgggaa aagcagaaaa gagcagaccg tgtagatgaa cttcttcaaa aagttggctt
     4561 atctcctgat atttatcgag atcgtatgcc tagagagttg tctggaggag aacagcaacg
     4621 gatcgggatc atccgagcta ttgctgcaag cccagatgtg attttgatgg acgagccatt
     4681 tagcgcatta gatccgattt caagaaactc attacaagaa ttggttttgt ccttacacga
     4741 agaattgggc acgaccatcg ttttcgttac gcacaatatg gaagaagcaa tcaagctagg
     4801 tgatcgtatt gcatttatga aggatggaga aattatccaa tgtgacacac cagagcagtt
     4861 attgatgaat cctaaaaacg actacgttcg gcatttcttt gatgaaccga aacagacgaa
     4921 agagtggcga gtagaagatt tagtcgtcaa tggatatttc ttaaatgaaa taccagaaaa
     4981 tgctcgtcaa caagtttgct ttgatacgcc tatgaaagaa gtatattcgc ttctttcggt
     5041 gtatccaagc attgtgatag tagaaaagca aagaagtatt gggtcgctga caagtaaaga
     5101 aatctttgct tttttaagtc gagaggagga aggaaatgaa aacatatgat gcaatcatca
     5161 tcggttcagg tgtgagcggc ttaagtgctg cttatggatt gaaagaagca ggtaaaacag
     5221 tactagtggt cgaagaagat ctatggggcg ggacttgtcc taaccgtgga tgtgatccta
     5281 aaaaagtctt gttaagtgca gtagaggcac gtaatcgggt aaaacaattg agtggaaaag
     5341 gttttaacga gatcccaact gccaactggg aagaacttca aaaattcaaa cgaacattta
     5401 cggatccagt cccagaaagc cgaaaaaaac aattagcaga agctgaaatc gatcatcttt
     5461 caggaaccgc tcgttttttg gatgattcat ctattgaagt caacgaagaa gtcttccatg
     5521 cggattactt agttttggca accgggcaaa gacctacgat cttaccagtg gagggaaagg
     5581 aatacttgaa aactagtgca gactttttat cattgcctgt actgcctaaa gaaatcattt
     5641 ttatcggtgg tggttatatt gcttttgagc tagctacgat tgccaatgct gcagggagca
     5701 aagtaaccat tgtccatcat aatcaacggc cattaaaaga atttgaggcg tctttggtag
     5761 aggaagccgt ccatcaaatg gaagcatctg gcattcagtt tgcttttggt gttgaaaccc
     5821 aaaagatcat ttcagaaggt actcgctatc gtttagtcgg aaaagagacg gaattagtag
     5881 cagatatgat tttttgtgcg actgggagac agccgaatac agaatcttta gcacttgagc
     5941 aagcgaatat cgtctttgat aaacacggaa ttgcagtcaa tgattatctg caaacgagca
     6001 atccaaaaat ttttgcgtgt ggagatattg tttcaaggaa aaccccaaaa ttgacaccag
     6061 tcgccacttt tgaaggaaat tatgtagcta agcggataac ggatgcaaca agcgaaccta
     6121 tcaaatatcc gattatacct acgatcgtct acgccagtcc taaacttgct gaagtaggag
     6181 ttacaaaaag tcacgcctct tcgtcagatc aagtggtaga gatggatctt accagttggt
     6241 tcacctatca tcgtgtcaat gaaccagttg ctaaggctga attgaccttt gaccagcaaa
     6301 attatttgat tggtgctgca gtgattagcg agcaagcaga cgaattgatc gatgatttga
     6361 cactcgtcat caatcaaaaa ttgactaaaa aagagttgga tagctatatc atggggtatc
     6421 cgacattggc aagtgatctc tcctatttat tgaaataatc agcatgataa ctaaagcgct
     6481 tgttgctttt tccttcttgt gctacacttt aaaaaatgaa tacctgtttc tggggatgtc
     6541 gtagacgaca tgagagtaag ttgtgaactt agacccattg aacctgtcag ttaatactgg
     6601 tgaagggaga aacatagcag acgcttgaag cgggttacta tgtcgtgccc atttcaagcg
     6661 ttttttgtta ttcatattag aagaaaggag gtaacaaaat gaagaaaaat ctttatccag
     6721 cagtcagtat ggcattcatt ttaattattt ggcagattgt tgtcatagtt tggaaaatac
     6781 ctgcctttat tttacccgga cctgctgctg tattttctgc attagcggca gatgcacaag
     6841 tattactttc ccattctgtc acgacattaa atgaagcagt gcttggtttg gtaatagcta
     6901 gcgttctttc ttttattact gccttggcaa tggattattg gaagttttta gaaatgtctt
     6961 tttatccgtt actagtgatt tcacaaatgt tgccaatcat ggtgttaggg ccattactta
     7021 ccctatggtt tggtttcggt atgcttccaa aagtgatcct tgttgtcttg ataagctatt
     7081 ttccgatcgt agtttctttt tcggatacat tgaggaaagt atcaaaagag caaatcatct
     7141 ttttgaaaac gatgggggca gatacaggga agatttatcg aatatataaa ataccagcgg
     7201 gtttatccgg cttcttttcc ggtttaaaag tggctgctac ttattgtgtc agcggagcag
     7261 tcgttggaga atggctaagc gctcaagcag gcttagggta ttacatgata cgcgtaaaaa
     7321 acagctacca gatcgataaa gtttttgctg cgatcatttg tgtcattcta ttgagtttat
     7381 tgctgaatgg cgcttgttca ttattgaaaa aagggtatta tcgtatgctt tatatttttt
     7441 aagagaaggg gatcaaatga aaaaattatg gcttttatta ccgctactgt tgcttttatc
     7501 cgcttgtggc actgcaaaag aaacaagtag caaacaagaa ataaaggatt tgaaaaaagt
     7561 gacacttgtt ttggattatg taccgaatac taatcatact ggcatctacc tagcaaaaga
     7621 aaaaggctat tataaagagg ctggtttaaa tgtccaaatc atcgaaccag gagataattc
     7681 gacaagtatt ggtctagtag gtgcggacaa agcacaattt ggtgtaagtt atcaagaaga
     7741 tgtcacttat gctcacgcag atggacagaa tataccagtc aaagcaattg ctacagtgat
     7801 caaacataat acttctggat tcgccaccct atcagacagt aacatccatt caccaaaaga
     7861 ttttgaagga aaaacatatg caggttggca aagtccaagt gaagaagctg tcttaaaagc
     7921 tgtgatggaa aaagacggcg gggacttttc aaaactgaca atggtaggaa gcaatggaga
     7981 agggccagaa agcttaggaa aatcctcaga tatccaatgg tactttgaag gttgggatat
     8041 gatcaaagca aaagaagcgg gtattgaact aaactatatc ccattgaaag aattagatga
     8101 acggttagat tactatactc cggtaattat tacgaatgat caactgatca aaagtgatcc
     8161 agaattggta cagtctttta tggatgccac gaaaaaaggc tatcaggaag ctattaagga
     8221 tccaaatgac agtgctaaac tgttgcaaaa atatgcaaaa gaaaatgatc ggacgttttt
     8281 agaagaatca caagcattct tgtcaaaaaa ttatacggat gatcctgaga attggggatt
     8341 gatggaagaa aaagtttggc gcaattatac agcttttatg caggaaaacg gattgatcaa
     8401 gcaagatgta ccttcgcaag aactattcac taatcagttt ataaaatagg agtgatagat
     8461 atgtcaatcc aaatcagcca cttgaatgta gtcattgaaa acctgaaaat attagaagac
     8521 atccatttca ctatccctaa agataccttt acatcgattg tcgcgccaag cggagccggt
     8581 aagtcaacat tgctgaaaac tttaacagga gtccagccta tgacttctgg aacgatcaaa
     8641 gtagacgatc aaaaagtaac tggtctccat acagcattta gttatatgcc gcaagaagat
     8701 atgttacttc cttggctgag cgtctatcaa aacgttacgt tatatcaaaa aatcaatcat
     8761 ctaacaatca atgaagaaca agtaagggat tatctggata tctttgggtt aaacggctat
     8821 gaacattttt tgccggaaca attatctggc gggatgaagc agcgaacggc cttgttacga
     8881 acaatcatga atccttcttc ttacttgctt cttgatgaac catttggagc gttagatgct
     8941 atgaccagag ggcagatgca agactggatg ttgaagctgc ctaaaaaagc aaagcggact
     9001 actttacttg tgacgcatga tatcgaagaa gcaatttatt tatcagacag gattcttgtg
     9061 ctttccgccc gtccggcaca tgtgattgct gagattcaag tgcctgaaaa acgacggtca
     9121 cgtgaatggc tactgcagca atcagagttg aagcaaacga tttatcagtt attggcaggt
     9181 gaattagatg ttaagtgatg ctcattgtca tttagatggg agtcagagtt tagcactcct
     9241 tcagcaggaa cacacgattt tgacgatcat caattgtgat tcgccagaag aatggaagga
     9301 aaacagacag cttgcagcaa gtaagactca agccttaagt tacggtatcc atccttggaa
     9361 agcagatagc tatacgtttg aacaagttga gccttttttg aaaaaagcaa ggatcatcgg
     9421 tgaaataggg ttagataata tttggacgaa tgtaccgatg actacacaaa aaaaagtatt
     9481 tgagcgacag cttgcttttg cagccataaa tgagaaacct gttgtactgc acacaaaagg
     9541 atgcgaaaaa gagatcttaa attatatcca agcttaccct aatcactatt tgatccattg
     9601 gtattcttct ttggactatc aacaagacta tattgattta ggctgttatt tcagcattgg
     9661 tcttgatttg aaaaagaatc cagcagtctg gcaactagct aaagcagtcc caatcgacca
     9721 cttacttatt gaaacggatg gactacatgc agcagagtgg gctttagggg ggattttttc
     9781 agaaaaagat tatgtctcac tgcttgaaga acagttaaag acaatcgctg agatcaaaga
     9841 gctatcagtt gaagaagtta aaaaaaatac ttatcaaaac ttggaacgtt tcattagatt
     9901 aggtaaataa aaaagagatg tgacacaact agtgtcgcac ctctttttgg ctttctttgt
     9961 ataaaagtaa actgtttgat taataaatga atcttagtac caaccgtttg cttcccagaa
    10021 tgctttcgcc gcttcccaag aaccataacg tgaagtaaca tattgttctg ctactctttc
    10081 ttggtttgca gcagagtagt caccatttaa gtaagaagaa tctaattggt aacgtccgat
    10141 gtaacgtccg ttcgttgctg tgtaagaacc gcttgattct ttttgtgcga tccattcttt
    10201 tgcagagctt gtgcttgctg gagtagcagc agtttctgtt acagcaggag caggttgtgt
    10261 ttcagcaaca ggcgctgctt gtttttccgt ttgaaccgtt tcttgtacaa caggttgttc
    10321 agcttggact ggtgcttctt gaacggctgc ttgaggttca gcagctgctt gtgcagagcc
    10381 ttctgttggg attgttaatt gttgtcctga gtaaatcagg ttaatatcag aaattgaatt
    10441 tgattctgca atggcattga tcaaggagtt atcaccaacg tatttttgag aaatcgttga
    10501 tagagtatca cctgattgaa cggtataagc ttcgtccgca tgagcagtcg ttcccatgaa
    10561 gaatgcagcg ccggcagcca aagttgttcc aaaaagtaaa gttttaagtg atgtcatgaa
    10621 aaatctcctt tattcgttaa aaagttttat ttgttgtgtt caacgaggaa tactttaaca
    10681 cgtgaaaata tttcataggt aaaagaagca tgacaattta tgacaaatcc gcgttttttg
    10741 taataaatac agtaatctat gaaatgaaaa gggttacgtt taataaaact tgcttttttg
    10801 tttttgttca ccataaatat ttatttaaga aagtattaac ctgcattttt ctctataaaa
    10861 aagatttttt aaatagataa aaaaggagga ttaaatgagt atttgatagt atttatatta
    10921 aaattacata tgtaatttaa aaacgcttga cattgtgttt gagatcactt acttttagca
    10981 tgtaaaagta ttggattaag gagctatgca aatgaaaaag aaatttatta ctggtgggat
    11041 tgtcgttgca tttctatgtc taattggcta cacaggcacg cggtacgcaa atgaaattat
    11101 tttgtggggt ggagagacag atattgatac aatcaatgag aatctgacac agcttgatca
    11161 tacattgaca gataaagagc aagcgattac agatcttacc gctcgactag cacaaaaaga
    11221 acagacattg caagattcaa aggcagatgt ccaagaatac caaacaaaag taaatgaatt
    11281 agaaaaccaa aaaaatcaat cggcagcaga aaaacaaaac ttagaaagtc aattagtcag
    11341 caaggatgca caactacaaa gcaaacaaaa tgaaatcaat gtcaaactag atgagatcaa
    11401 ccaaaaaaat gatgaaatca atcatctgaa tgcagattgg acagcaagac tacaagaagc
    11461 acaagcaaaa ctttcagaat tacaaaatac tgtaaatggt ttgaatcaac aaatcgcaac
    11521 actgacacaa gaaaaaacaa atgtagaaac tcagttaaca gatacacaaa agaaattaga
    11581 cgaagcaact gctgaaaact caagcttgaa acaatatatt caaaagttag aaaaagcaaa
    11641 agatgaagtg gaagatacag ctgataaatc tcagcaaatt gttgaagaac atttgcgtaa
    11701 ataatctttc ttaacaagta aacctaaatt agtaaagaag caacagtaat ctatactgct
    11761 gcctcttttt tttagtacat gtccttcatc ttcgcaggaa gtgccaatat gattttaaga
    11821 aaggtaatag acaaaaagtc aaaaagataa aacagagttt cttctttaca aagggaatgt
    11881 atgttcgtat aatggtattg aggtgaaggt tatggagaaa aaatatggaa cagtagataa
    11941 gatccgtact ttgcaagtta gtgccagacc gttgatccgt ttcagtttaa atggggtcaa
    12001 ttgtttgatt gcttcccata gtttgaactt cctagctgaa gtagacgaag ggatgaagtt
    12061 agtcgttaca ggttattata ataatagaaa gcaatttgtt gtgagatcct atcatgtgtt
    12121 agggaaaccc aaaattgtgg tagaatatga gaaaagtcta tacccaagaa aaaaggtccg
    12181 acaagatgaa agaataaact agaaaacaag cggctgagac aaaaatgtcc tagccgcttg
    12241 ttttggaaag atggatgcag acgatgggat ttgaacccac acgagcatac gctcacaaga
    12301 acctcaatct atatataact acatgtattt aagtatttgt gatgaatcac taaactttcc
    12361 aaactcattt gagggatatt gcttaaagat tgaacagatc aattcgaagc cctattagta
    12421 ttcgctcaat ggggggatca gaggccataa aaagagacta gtcattacta gtctcaggta
    12481 gtggaattgt aggattttaa acgttcctgg agagtaccgg tatgtaactc gaagaggtga
    12541 ttatcatagt cgtaaaagta aattgaagaa gcctcgcctt tcacacgaga acgtcctggt
    12601 tcgacagtta aacctaacat ttggattttt gagacaaacg aatctatatc ttgttcgtca
    12661 attttaaaag caatatgatt gtatgtcttt ggcagtttat tcgacgaatt ctgcattact
    12721 gcaatccata ggtccttaac taagaaaaat ttttctggat ataatgaaaa tttttttgtt
    12781 gactatcgta gatttctttt gcatcaaata aggtatttaa caattaagta tttaaaagat
    12841 aacttttaag ttatctttaa gtaatgctta aagctatgtt atactctatt aggaaatata
    12901 agaaggagta taaaaatgaa caaacataat agaaaaataa cgaataacaa agaactgatc
    12961 ttgttactaa tagggatttg gggtatcttt ttactcttta atatgtatat gccgactatg
    13021 agagcggatg acgtagttta tgcaagtaga ttagatgaac ttggctattt gggtgcttcg
    13081 atagaacatt ataagacatg gagttcaaga attattatag aactattctt aatgttcttt
    13141 tctaaacact ttatgctatg gaaattatta aattctacga ttatgttagg aattgtagta
    13201 ttactttgca agtat
//
LOCUS       DAZABD010000073        13148 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000073 DAZABD010000000
VERSION     DAZABD010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13148
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.073"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(79..151)
                     /locus_tag="ACHALG_002302"
     tRNA            complement(79..151)
                     /locus_tag="ACHALG_002302"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(116..118),aa:Lys,seq:ctt)
     gene            532..1101
                     /locus_tag="ACHALG_002303"
     CDS             532..1101
                     /locus_tag="ACHALG_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HFN2587665.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1088..1948
                     /locus_tag="ACHALG_002304"
     CDS             1088..1948
                     /locus_tag="ACHALG_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFN2587666.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2138..2842
                     /gene="yycF"
                     /locus_tag="ACHALG_002305"
     CDS             2138..2842
                     /gene="yycF"
                     /locus_tag="ACHALG_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HFN2587667.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2847..4682
                     /gene="walK"
                     /locus_tag="ACHALG_002306"
     CDS             2847..4682
                     /gene="walK"
                     /locus_tag="ACHALG_002306"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HFN2587668.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4679..5995
                     /gene="yycH"
                     /locus_tag="ACHALG_002307"
     CDS             4679..5995
                     /gene="yycH"
                     /locus_tag="ACHALG_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system activity regulator YycH"
                     /protein_id="HFN2587669.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            5996..6865
                     /locus_tag="ACHALG_002308"
     CDS             5996..6865
                     /locus_tag="ACHALG_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HFN2587670.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            6920..7729
                     /locus_tag="ACHALG_002309"
     CDS             6920..7729
                     /locus_tag="ACHALG_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFN2587671.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(7832..8461)
                     /locus_tag="ACHALG_002310"
     CDS             complement(7832..8461)
                     /locus_tag="ACHALG_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFN2587672.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8631..9923
                     /locus_tag="ACHALG_002311"
     CDS             8631..9923
                     /locus_tag="ACHALG_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFN2587673.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(9999..10661)
                     /locus_tag="ACHALG_002312"
     CDS             complement(9999..10661)
                     /locus_tag="ACHALG_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFN2587674.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            10886..11170
                     /gene="groES"
                     /locus_tag="ACHALG_002313"
     CDS             10886..11170
                     /gene="groES"
                     /locus_tag="ACHALG_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFN2587675.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11221..12846
                     /gene="groL"
                     /locus_tag="ACHALG_002314"
     CDS             11221..12846
                     /gene="groL"
                     /locus_tag="ACHALG_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFN2587676.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4371 a         2265 c         2833 g         3679 t
ORIGIN      
        1 aataaaaaaa gaacagcgta aatgttttcg aaatgctttc gcaccaacat ttacctgttc
       61 cgtgggtttg gcacccaatg ggccgtgagg ggctcgaacc cgcgacccgc tgattaagag
      121 tcagctgctc taccaactga gctaacggcc cgtacaaaaa gtacttaata atagtagcac
      181 taagaatttt aaaatgcaag actacattta aagaaaatca gaaaaatctt tacttcctta
      241 taggaaatca ttcatagatt attcacattt gctcgctatt atataagcat accaacaaac
      301 aaagttcttg caagagaaca aaccctaaca cttttttcat tttttactcc tttcccgccg
      361 tctccccggc gggtctcctg ttcttaacga taaaagacaa gaaggcttag ccttcttgtt
      421 ttttgttttc atggaatccc gctaatcaaa tcacctagtt aaagcaacta gattgaatgt
      481 ttatcataag tctgatatca tgtttatgaa atgaaaaaag gagcggttat gatggaaaaa
      541 atgcgtcagc aattaatgga gtgggggaag gaactagaaa cattccgtct gcctcactgg
      601 gaagaactcc ctgacttaga actttatatg gaccaagtca gaacgttagt tgatcgttat
      661 ctttcacctg tgatacaagg agaaaagcat ccattgctta cctcttctat ggtaaacaat
      721 tacgttaaac taggtttgat accagcacca gttaaaaaaa gatataacaa agaacacgta
      781 gcatttttac ttgcaatcac tacgttgaaa caagtattga ctattcctga aatcaaagaa
      841 gggatattgt tccaaggaaa aacagtagga attcgtgaag cctataatct attttgcgat
      901 gaacaagaag cagctgtgtg gatggtcagc caattagcac aaggtaaatc tcatccgcaa
      961 aaattcgacc aggcaacccc tgtagaatat attgctgtaa gagcggcaac tttatcgttt
     1021 gcaatgaaac ttcttgcgga aaaaacgatt gttctagaaa ccgaatattt aaaagaggag
     1081 aaaacaaatg aaaaacaata aaatcgcaat tttagtagat tcaggtacgg atgtgccaga
     1141 agaattgata aaaaaatacg agatgtatat gatcccattg aaaattattt ataaagatca
     1201 ggtatataca gataaaattg acattacctc tgaagagatc tatcaacgtt tgccagagga
     1261 aatccctggt acatctttgc cggatggtga gtcgatcaca gaaatctttg agcaaatcaa
     1321 aaaagacggt tatgaaaagg tacttgcggt aacaatttcc agtggattga gcgggacata
     1381 taatgtggtt cgtttaattg cacaacagca agaagaattg gaagcatatg tactagatac
     1441 gaaaaatatt ggtattggtg caggtttttc tgcgatccaa gcagcaaaat ggctggaaga
     1501 aggagtagaa tgggatcaac tgatcagtaa tctgaatgag ttagtcgaaa gaacaaaagt
     1561 tttctttaat gttgcgacat tggaatatct tcaaaaaggt ggacggatcg gtttagttgc
     1621 ttctattgta ggaaccgctt taaagcttaa cccaatcatc tcatgtaatg aagaagggat
     1681 ttattacaca gtaggaaaag ctaggggacg taaaaaaagt ttggataaaa cggttgctta
     1741 cgtcaaagaa aaaatcggta atgcaaaggt cttcaactta gctgttgccc atggggatgc
     1801 gaaagaagaa gcaattgaaa tggaagcgag acttaaaaga gagttcccac aagcaaacca
     1861 aatctacttt ggccagattt ctccggcatt agtagtccac actggtccgg gattattggg
     1921 tgtcggtgta caagttttag aaggataaat caaaaaaagg aaacactgag gtgaaaatca
     1981 gtgttttcct tttttattga agttagaaca aaaaataata aagtattttg cactttcatt
     2041 ttaattttct cttttgtttt ataggcaaag atgaaacaaa agcagaaata cggtataatg
     2101 agaaggtatg acaataatat ataagaggtg gctctttatg aaaaaagttt tagttgtcga
     2161 tgatgagaag ccgatttcgg atatcgtcaa attcaattta gccaaagaag gctatgatgt
     2221 ttatactgca tatgatgggg aagaagcctt ggaaaaagtt gctgaggtag aacctgattt
     2281 gattttactt gatttgatgc tacctaaaat ggacggctta gaagttgctc gtgaggtacg
     2341 caaaacttat gatatgccga tcattatggt aacagccaaa gattctgaaa ttgataaagt
     2401 cctaggctta gagttaggcg ccgatgatta cgtgaccaag ccattttcca accgagaatt
     2461 agttgcacgt gtcaaagcaa atcttcgtcg cggtgccacg gctgcgaagg aaccagaaga
     2521 ggcagcacct gcggaattga cgattggtga tttgacgatc catccagaag cttacatggt
     2581 gacgaaacga ggagaaacaa tcgagttaac acatcgtgaa ttcgaactac tcttttactt
     2641 ggcgaagcac cttggacaag tgatgacaag agaacattta ttacaaacgg tttggggtta
     2701 tgattatttt ggagatgtac gaacagtgga tgtaactgta cgtcgtttgc gtgaaaagat
     2761 cgaagataat ccaagccatc caaattattt agtgacacgt cgtggtgtcg gttactattt
     2821 gcgtaaccca gaacaggagt aattggatga aaagaaaagt ccgctttttt cgatcagtca
     2881 attttaagat cgctattacc tttattctga ttttattgat ctcgatcgaa attattggtg
     2941 cctactttat acgtggttta gaacgttcca cgatcaatac atttattaaa gatatgaatc
     3001 aaacggtgga atcattagca acaacaatca gcccggaatt gaatcgaaaa gataatgcag
     3061 atgatgaaga ggtaaatgcg aatatcaaaa gatttattga aaacagtgcg actagcgata
     3121 tcatcgaaat acgggtcgtt gacgaaaaag ggatcatccg aggaacaacc gatgtcaatg
     3181 aacaaagcgc tgtgggaaag aaaaatgatt atgttgatat caatgatttc acgactaaac
     3241 gttatgttgc tttagataac gacgataaac gggtgaatat caatgttcaa ccgatcctgt
     3301 ccccgaccgg cgatgccgtt atcggggctc tttacgtcaa aagtaatatc gaacaaaaat
     3361 attcggaaat caataacaca gcagtcattt tctttactgc ctcactaatt gcggcattta
     3421 tttcaatgat cgtatctgtt ttagttgctc gttcgattac acagccgatc ggtgaaatga
     3481 gagaacaagc cattcgaatc gctagaggag actacagtcg gaaagtcaaa gtgcatggac
     3541 aagatgaact tggacagctg gctgatacct tcaatcagtt agctgaacgt attgaagaag
     3601 ctcaagatac aatggaagct gaacgcaatc gtttggatag cgtgctatcc catatgacag
     3661 acggcgtcat tgcgacagat cgcaggggta aagtcattac gatcaatgat atggctgttt
     3721 ccttgctaga tatcaaaaac gaggaagcaa tcggacaatc gattttgaca cttttagata
     3781 tcgaagaaga atatacgttg cggaaattat tagaagatcc aaacgaaatg gtacttgacc
     3841 gttcaaccag tacattggaa agtgatcaga tgatcttgcg agtagacttt tctatgatcc
     3901 gtcgcgaatc aggttttatc agcggattag tagctgtctt gcatgatgtg acagaacaag
     3961 aaaaaacaga acgggaacgt cgtgaattcg tctctaatgt ttcccatgaa cttcgtacac
     4021 cgttgacaag tatgcgtagt tatatcgaag cattgagcga aggtgcgtgg aaagacgagg
     4081 aagtagcacc aaattttttg aaggtaacgc tagatgaaac agaccgaatg attcgaatga
     4141 tcaatgacct cttaaatcta tctcgcatgg atacaggtaa tacccagctt caattagaat
     4201 atgtcaattt caacgagtta gtgaattttg ttcttgaccg gtttgatatg atggtgggaa
     4261 atcaggaaaa aaattataag atccgtcgtg aatttacgca aagagatctt tgggtcgaag
     4321 tagatacaga taaaatcatc caagtagtcg ataatatcat gaataacgcg atcaagtatt
     4381 caccagatgg gggaacgatt acttgtcgct tattagaaac gcataataac gtggttttaa
     4441 gtatcaccga tcaaggattg gggattccta aaaaagatct gaaccgagtg ttcgagcggt
     4501 tctatcgagt agataaagca cgtgcaagag cacaaggtgg gacaggactt ggattagcta
     4561 tttctagaga agtgattaag gcgcatcgag gcgctatttg ggcagagagc aaggaaggaa
     4621 aagggtcgac tttttatatc tcgctgccat atgaacctta tgaggaggaa tggtgggaat
     4681 gagaatctct gaaaaagtcg ttcgtatagg actcattttg atggttgcgt taagcatata
     4741 cttctcttat gcaatctggc tgagcccagc aggaaaaagt tcaatcaacg tagatgagac
     4801 aaacactcaa atgatcgagt ctcaaagcaa tcgaaaaact tccgaagtct ttcttccgct
     4861 tcatgttaca tggcaccatt cgggaatgat ccaagaaacc aacagtgaaa atctcgtgta
     4921 ccgattgcaa ttgatcatcg ataaagcaag atttggaaag ctttctgaag tagtttcagg
     4981 agacaaatca cagtttgaac aggtgaaaaa tctgaactct ggttttgagt tggcctataa
     5041 tgcgcctttt ttgctaagag aatacaagga agcatttgat ttagatttag atgtgtcgaa
     5101 cttggcaaaa gaaagcgaaa atatgtattt tactagaata caattcgatc aaggaaataa
     5161 aaaaatccga tttttgaatt attcgaacca tctcgtatat gaagcaaata tttcgttgaa
     5221 ttggacagat atcaataaag aattaacagc aaaagacaca aaatggacag aaatgacaac
     5281 agaaccatcg acgatcgaca cgcaatatgc tacgaaacat tcgatcaaac tgaaaaagta
     5341 tagttatatc ctgtcacagc aaccctatac actttttaga aatgcttttt tccagaaacc
     5401 agatagcgtg aaaaataatg atgaaagcaa tgaattgatt ttttacggtg ggaatgaaac
     5461 aatgtctatc cattcggaaa cgcaagtagt tgattttcgt ggagagctgc cagagaaaaa
     5521 agaaaatgct agtgtatttt ctgaaagttt cccctatatt agcaaattag gcagttcttt
     5581 agggaacctc agatattttg accgcagtga caatacgatc gattatcgga tattcgtgga
     5641 aggcttcccg gtttttggga cagacagcag aggacagatt ggtgtggaag ttggcggtga
     5701 aacgaatggt tctgtgcaag tgaatatcca aaccagtttg aatacgattc aagtgccgat
     5761 accttcagac gaagaagtag aacttgcaag tacgaatgaa atcatccagc agttaaaagc
     5821 taagggagca gatagcaaca agattggttc agtgatcgtt ggctatacat ggcaaaatgt
     5881 caaagaaacg aatcgtgtcg ttgatctctt acctgaatgg tatgtgaaat ataaagataa
     5941 ctggtattca gctaacgacc ttctggaaaa attacctgga ttggaggcga attaattgga
     6001 tttcaaaaaa atcgaatgga tatttttcgt cgctttttta gggttgaatg tgtttttatt
     6061 cagcagttat catgacgcgg tataccaaga aaacaatgtc attcgctcta atcagactga
     6121 atcaatccaa caacgtctgg ctagcgacga tatcacttac acggggaaga tttcgtctga
     6181 gaacaagcaa ggctattatc ttagcgcgga gcagaccaat cttagcactt cattagcgaa
     6241 ttatcgcaag gctggtaaaa gcggcttgct ggcaagtggt gtgtttttag acggtgatgt
     6301 tttgacgaaa tctttatcca gttcagatgc tgaaaagaac gtgatcgatc cagataaaat
     6361 tagtagttcc ttggaagatt ttttatctaa caaagacaat attttattcg gtaatgatta
     6421 tgtgtacacg aaaaactttt ctcatttaga agaagaacca ctagaaatcc aagcaagcca
     6481 agagtatgaa ggcatcccat tcaatgatga tacagcaaaa atcgttcttt ctttagaaaa
     6541 aaacgataat gattatagca tcacgaaata catgcagacg catttatctg atatcgagca
     6601 gctgcgcgaa aagacggaac tgtatacgga agaggaagca atcaatacat tgtacatcaa
     6661 taacaaaata ccaagaggat caaaaatttt atggcgtcag ctagcttatt cctgtattct
     6721 gaaagtccgg gaaaaaaatg tgtatgttcc tgtttggcat gtagcgatcg aaacaagcga
     6781 taaagtggtc caaattgaga gtgtcaacgc tttcagcaat acgatcgtga caaacaatac
     6841 tgtaccaaaa gtggaagatc aataaaaaag cgagtataat gaaaaaagtt tacaaagtaa
     6901 gaagggaagt aggagtcaaa tggctgaaga aaaaccagct tttaaaatca gtatcctagc
     6961 aagtggaagt tcgggtaatt cactttatat cgaaagtgaa aaaaaacggc ttttggtcga
     7021 tgcaggactt agcggtaaaa aaatcacttc cttgatggct caaattgatc ggacaccgga
     7081 ccaattagat ggaatacttg tgacgcatga acatcgagat catatacatg gcgtgggtgt
     7141 cttggctcgt aagtatcatt tggatgtttt tgccaatcag cagacttggg aagcgatgga
     7201 accactgatt ggaaatgtac cagtggaaca aaaacatcta tttgagatgg ggaaagtcag
     7261 aacattcggc gatatcgata ttgagagttt cggcgtctcc catgacgcag cggctccgca
     7321 attttatcgt ttccataaag aaaatcggtc attcgtcatg ttgacggaca ccggatattg
     7381 cagtgattat atgcgaggtg tcattgaaaa tgccgatggt tatttgatgg aaagtaatca
     7441 tgatcttgag atgttacgca tgggacctta tccgtggaat ttaaaacagc gtattttagg
     7501 agatagagga catctttcca acgaagacgg tgcacttgtc atgactgatg tgatcggtaa
     7561 tcggaccaaa cgaatctatc ttggccatct tagcaaggaa aacaatatga aagaacttgc
     7621 tcatttaaca atggaaaatg ttttgaaaga aaaagatttt ggcgttggac atgattttga
     7681 aatctatgat acagatccag atgaagcaac tgagctattt tcgatttaaa cagttcaatc
     7741 aataaaaata taaaaagcag aagataaaat caatcagggt tggttttatc ttctattttt
     7801 atttttttag catattaaaa aaataaccgt attatcgact gactacactg atgtgctgct
     7861 tttcatgttt ctcatccagt atctcatcta ctaatgcaat cgcgtaatct gccatgctga
     7921 tcgtactgtc gccagcagcg tttctttgta aataatcatc acttaacaaa taagtacctg
     7981 tacgtttgcc atgtggatca aataaagaag ctggactgat ataagtccaa tgtagatttt
     8041 tagattgttt caattttaac aatccttcgt acatattgaa agctgttgga tagaagggtg
     8101 catttttagg tacttggtca atcatacgag tcgtcttttc tgtatctaag aataacgaag
     8161 aggcaccccc aacgacaatt aagcgtgtat cggttccttc aagcatctca atcaaatggg
     8221 acaaagaagt ttgatgcatt tcttcttttc ctttgggagc gttgaaagca tcaacgacaa
     8281 catcaaaaga tgcaagatca gtagttgtca actcaaataa atccttttgt ataaacggaa
     8341 catctgatgg caatttttct ggatgacgca cgattgccgt cacgtctaaa tctctggaca
     8401 atgcttcgtc taaaataaat gaacctgcat ggccagtagc tccaatgagt gcaattttca
     8461 taaaaaacct ccttttttat tcctgtccat tgtaagcagt tgctgaatat tcgtcaaaga
     8521 atccgatttt tgaaaagctc agtgcgcatt cctttcatgg ttcgtgatca ttgggtatag
     8581 ttaaattaag aaaactgatt gataaaaata gaagttgaag gagataagaa atgaaagagt
     8641 ggttagatcg tgccttttta ccaatgataa catctgttgt gaatcttaga cccttaactg
     8701 ctttgcgtaa tggaatgact tacacgatcc caataattat tgttggctcg ctgtttttct
     8761 tatttgctca ttttcctgtg gattacatcg ctcagtggat agacaaaaca ggattttcta
     8821 tgtattttac acaagtatac caggctacat ttgatgtgat agccttttgg atcgtttttg
     8881 ccattgcata cgcttatgca aaagaagaag acatcgatcg cttaccagtc ggcataacag
     8941 ccttgtctgc ttatctactg cttattcagc cactaaatac tcagatagat actgtctgga
     9001 caggtgctga agggatacta ggagcagtta caactgggct catcatcgga tggggctatg
     9061 cacgtttagt aagacttgcg aaagggtgga agcaaagaga gaagatacct gaaaatgttt
     9121 ggcagtcttt tacttcattg attccaatca ttgttatagt cacttttacg cttatcggtt
     9181 cagcggtttt tcaacagttg ttcagaatga ctgttgttca aggagtatgg acatatgtcc
     9241 agcttcccat tcaatcgttc atagacacgt tgtttggtgt gcttttatta ggtttttttg
     9301 ttccgttttt atggttattt ggtcttcatg gatctgcgat ggtcaatggg ttggttagtc
     9361 ccgtcttgca agcgaattca ttagcgaatg cagaaatctt agcctcagga caagagttga
     9421 cggtggcaaa tggcggacat atcgttactc agcaatttct cgatcagttc atgacggtta
     9481 cgggtgctgg attgacgtta ggggctgtat tttttatgac ggtttttgcg aaatcgaaga
     9541 aatacagaga attaggaaat cttgctcttt taccagcctt tttcaacatc aatgaatcaa
     9601 ttattttttc cacaccgatc gtgatgaacc cgatgatggc tgttcctttt attttcgcac
     9661 cggtcgtttc aggattgatt acctattccg ctttatattt tggattcgtt cctttattta
     9721 cagcagtcca agtcccttgg acaacaccgc cgatcctctc gggtttctta gtaggcggag
     9781 tgcctgcagc gattttacaa ggtatcgtgt tgtcaattag ttttttcatt tatttcccat
     9841 ttttgaaaaa aatagattct gcaaactgca aaaaggaaag acagacaaaa aatgatggag
     9901 cagcagaaaa gatgaacgac taaaaaacaa aaatactcct gtcgctttag attgggaaaa
     9961 tcaccaatct aggatgacag gagtatttat agtcatgttt atactagccc tttttggaca
    10021 gctaattgaa caagaacaac taatgtgttc attcctacgt gtgtgatcat cgatgtccag
    10081 atccgaccag ttgctttgta ctctaggctg aagaagaaac ctaggaaaaa gtagattagc
    10141 agatgaccat cattatgagc aaacgcaaac agcaaagagc tgatcactgc tccgacccaa
    10201 aaatttgaat atcgactgat gatccccagt atactgcggc ggaatacgaa ctcttccatg
    10261 atgggtccag caacagttgt cgccaaaata aaagcgggtg cttccatgat catctggatg
    10321 atattttgtg tattctcgga aggggttgct ttgccaaaca atacagattc taacatgaca
    10381 gcgatgcttt gcagaatcaa tgcgacaaag attccaacca atccataaat aacaatcttt
    10441 ggagcagaag ctttttttac cgctgattcg atcgctaagg gttcacgttg cttaaaataa
    10501 aaaatagcta ggacaacggc gcctagtata tacatgactg cagttgctgt gatcaattca
    10561 gacgtagatt ttacaagtcc tatcgaagct aggatcaagg gtgagataaa aattagacca
    10621 tagcaaaaaa tgctgaggaa gctatatttt tttaaagaca tgtaattcct ccttttaagg
    10681 gataacaagc atgcaaggca agcttatatc gattgatttc aattatatca taatttcaat
    10741 aagcagctgt cagaataaaa tttcttttct ttgatttaaa agcttgcaaa aaagagggag
    10801 aatgagtatt attaaaaatg taggttagca ctcgaaggtt aagagtgcta acagattaaa
    10861 aaaagagtta tcggagggat ttatcgtgtt aaaaccatta ggtgatcgcg tcattatcga
    10921 agtcgcaaaa gaagaagaaa aaactgtcgg aggaatcgtt ttagcttctg ctgctaaaga
    10981 gaaaccccaa actgggacag ttgttgcagt cggtgaaggc cgtttgttag aaaacggtga
    11041 aaaagttcct gccgctgtaa aagcaggcga ccaagttatg ttcgaaaaat acgccggtac
    11101 agaagtaaaa tacgaaggaa aagaatatct gatcgtcgca ggaaaagata tcatggcgat
    11161 tgttgagtaa atttggaaga taaaaaaata atcaaaaata atcaatgagg tgaatcaatc
    11221 atggcaaaag aattgaaatt tgcagaagac gcacgcgcag ctatgttacg cggggtagat
    11281 aaattagcag atacagtgaa agtaacatta ggacctaaag gccgaaatgt cgtactagaa
    11341 aaatcttacg gctctccatt gatcacaaat gatggtgtga cgatcgcaaa agaaatcgaa
    11401 ttagaagatc attttgaaaa catgggtgca aaacttgtat cagaagtagc atcaaaaaca
    11461 aatgatattg caggagacgg tacaacgaca gcaactgttc tgacacaagc aatcgttcgc
    11521 gaaggtttaa aaaacgtaac tgcaggtgct aatcctctag gcattcgtcg cgggatcgaa
    11581 ttggcaacaa aagcagcagt cgaagaattg cataatatct caactgttgt tgattcaaaa
    11641 gaagccattg cacaagtagc agctgtttct tcgggttctg ataaagttgg acatttgatt
    11701 gctgatgcaa tggaaaaagt tggaaacgac ggtgtcatca caatcgaaga atcaaaaggg
    11761 atcgaaacag aactagatgt tgtagaaggt atgcagtttg atcgtggtta cttatcacaa
    11821 tatatggtaa cagataacga caaaatggaa gctgttctag aaaacccata tatcttgatc
    11881 acagacaaaa aaatctctaa catccaagat attctgccat tgttagaaca aatcttgcaa
    11941 caatcacgtc cattgttgat tattgctgac gatgtagatg gtgaagcatt gccaactctt
    12001 gtattgaaca aaatccgtgg aacattcaac gtagtagctg taaaagctcc tggtttcggc
    12061 gatcgtcgta aagcaatgct tgaagatatc gctattttga caggtggtac agtaattact
    12121 gacgatctag gtttagaatt gaaagatgta acaatcgaaa acttaggaaa tgcttctaaa
    12181 gttgttgtag ataaagacaa tacaacgatc gtagaaggtt ctggagaaaa agaagcaatc
    12241 gaagctcgtg tgcaattgat caaaaaccaa attgctgaaa caacttctga ttttgaccgc
    12301 gaaaaattac aagaacgctt agctaaatta gcaggtggtg tagctgttgt taaagttggt
    12361 gcagctactg aaacagaatt gaaagaatta aaattacgca ttgaagatgc attgaatgct
    12421 acacgtgccg cagtagaaga aggaatggtc tcaggtggtg gtacagctct agtgaacgtt
    12481 atcagcaaag tttctgctgt agaagcagaa ggagacgtgg caacaggtat caagatcgtt
    12541 gttcgtgctt tagaagaacc aattcgtcaa atcgctgaaa acgctggtta tgaaggatca
    12601 gtgattgttg ataaattgaa gaacgtggaa ctaggcactg gtttcaatgc tgctactgga
    12661 gaatgggtaa acatggttga agcaggtatc gtagacccaa ctaaagtaac acgttctgcc
    12721 ttgcaaaatg ccgcttccgt ttctgccttg ttattaacga cagaagcagt cgttgcagat
    12781 aaaccagaac cagcagcgcc agcagctcct gcaatggatc catcaatgat gggcggtatg
    12841 atgtaagaaa aagcaacttg cttatctaca actgatcatt agtgaaagac agataaaaag
    12901 aggctggaac agaagcgttt aactccaaga aataagaaga aattcacgga aattgctttt
    12961 caaatttttg tgaatttcag cttatttccg aaggagttgc ttctgcttcc gctgtttata
    13021 cgtgtttaga gcgtgagaca aaagtgttct ttacttttgt cccacgctct tttttgtgtg
    13081 taattataat gaaaatagtt atttttaata gttataatat tttatctatt aaaagtaaat
    13141 atccattc
//
LOCUS       DAZABD010000074        12724 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000074 DAZABD010000000
VERSION     DAZABD010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12724
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.075"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(103..184)
                     /locus_tag="ACHALG_002315"
     tRNA            complement(103..184)
                     /locus_tag="ACHALG_002315"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(149..151),aa:Leu,seq:cag)
     gene            complement(526..1419)
                     /locus_tag="ACHALG_002316"
     CDS             complement(526..1419)
                     /locus_tag="ACHALG_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HFN2587677.1"
                     /translation="MKWRGGRQSSNVEDRSGRSAGSGGKIMAGGGIGTIVIAVLFFLF
                     SGGDLNSVTDILGGGQAANYQTENVASDEYTEEKEFSAVVFGHLEDYWNGVFEAEQTT
                     YQDPKLVLFTGSVQSACGNATSQVGPFYCPADQNVYLDLSFATELSQKYGASGDFAMA
                     YVIAHEVGHHVQNELGITQQLDKIRQQVSEKEYNQYSVRLELQADYLAGTFAKYLQGK
                     TYNGQPILEAGDIEEAITAANAIGDDTLQKEYQGYVVPDSFTHGTSKQRVEWFNRGFK
                     YGDLEHGDTFNEKNLDLVYGS"
     gene            1633..3270
                     /locus_tag="ACHALG_002317"
     CDS             1633..3270
                     /locus_tag="ACHALG_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003125786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFN2587678.1"
                     /translation="MKNNYSLIEDRRMQIFKRLINGEHLSYQQLSDEYYVSRSSIAKD
                     IAYLKTLFVKENLLLRFDNSGTYFQGSESQIQRMLKRFILLTVEHGENLSVLTNVSLF
                     EKINAAFRHSITEKQMEIPESYIQSIVVSILLIVERSNQANQLDIEEKRQFGKLFLEF
                     DKYPLIYELLKEIEEQEIYHFSSKEVQYLTYLIVGSGLRFFMKNESIPFSFRGKVRNL
                     IQKVSEGIQIDLTQDSRLEEDLTIHLYQLFLRIEAQTTVVNPLLDEIKQHYPALYGVV
                     WFSLDDFLKAYHIGISEDEIGFVSIHFQAAVERMRQLNKIVFVCPNGVGTSSFISAKI
                     RRILPNVDSIETASVAKLKEMDISAIDFIISTIDLPDVSKPVVRISPMVTNRDMKRIM
                     NHYIDLIIDNEPIADKVKILQDTRMLIAPKIYFERFETKEQALDFLISQNDFSSENQR
                     TDFQRSVLEREALQSTYLDNGFAIPHGNPEFVSKTTFSVLILDRPVDWGNQKVDIIVL
                     LMIREDEAQEVEPVMKLIMQGIEDKEWFISKMLEVKS"
     gene            3267..3698
                     /locus_tag="ACHALG_002318"
     CDS             3267..3698
                     /locus_tag="ACHALG_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFN2587679.1"
                     /translation="MINLKKLLPEDHIETTNQILTWQEAVQLASQPLLNEKAIDQQYV
                     ANMIHSVEENGPYMVLADYFALMHARPGEGVFHQGMSLLVTKNEIDLAGKSVRIFLVL
                     AAKDNQSHLESLQEIMEVFMDQEKYQLILNGDKEQICQLFQ"
     gene            3711..3992
                     /locus_tag="ACHALG_002319"
     CDS             3711..3992
                     /locus_tag="ACHALG_002319"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFN2587680.1"
                     /translation="MKILAVCGFGVGSSMVLKMTIDKVIKELGLKATAENTDLSTAKA
                     TPADVYFTSHELLNDLQASVKAPVYPIKKYMDVAEVKMQMEKFLAEREG"
     gene            3995..5245
                     /locus_tag="ACHALG_002320"
     CDS             3995..5245
                     /locus_tag="ACHALG_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFN2587681.1"
                     /translation="MSFVTDHILKNPPVLLGLIAMIGLIVQRKPFAEVVKGSLTAAFG
                     MVALTAGVNMLTGTIAPINTAVQTQLGVQVADGLSDVTFTAEYGGTVGLAMFFGLILH
                     LLIARFTPVKTIFLTGHMLWWFPFVIVAGGVEGGMSGIPLLILGAVLSACYWSFMPWI
                     MRKYVWDATGDDSFLIGHPTGILSMVSGFVAKRVGNKEKSTEDLKVPENLSFFREISI
                     TGALVMFLMNIVVGIIAPVLVPEGGNLVMFAVQAGLNFGAGLLIMLYGVRLLINQIIP
                     AFQGIAEKVVPGAKPAFDVPILFNYRPNAVIIGFIVAMITSTILVIIVNTTNVFGILI
                     VPLVITSFFECGGAAVIGEGQGGLRGAVIGTIAASFVMVLLVGVSAVLFSTTIQNWIL
                     IFGGNDISLWGIIGRGLGSLFGGI"
     gene            5247..5984
                     /locus_tag="ACHALG_002321"
     CDS             5247..5984
                     /locus_tag="ACHALG_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008379144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar isomerase domain-containing protein"
                     /protein_id="HFN2587682.1"
                     /translation="MYQYVDKIHALLETVEKKEQHAIDEAIRLLTTANLEKHSIYIFG
                     ASHAGILAEEMYYRAGGMMTINAIFGRELMLDRSPITFTSKMERLEGYGTALAEIQPF
                     KEGDVLILHSVSGRNPVIIDLALAAKEKGVKIIALTNKTYSQSVTSRHSSGLRLFEVA
                     DLVIDNHGEIGDAGCQIKGMDQKVGPTSTVIGATILNTIIVEVCQQLKQAGIDYPPIF
                     YSANMDGGDELNQSLFEQYKEAIHYQL"
     gene            complement(6236..7075)
                     /locus_tag="ACHALG_002322"
     CDS             complement(6236..7075)
                     /locus_tag="ACHALG_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003125793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFN2587683.1"
                     /translation="MKAPLLTLNDGNMRPAVGIGTYQIRGGEGLSQLQAAIADGYRLI
                     DSSTNYDNEGIVGKAIRRSGVPRSEFYITSKLPGKYHGYDDALTSIQESLARMGLDYF
                     DAFLIHWPLPKRGKYIEAWQALVTAQKLGLIRSIGVSNFEPEHLDAIIEATGVTPAIN
                     QIEIHPYWVQEQMVAENRKRGIITEAWSPLGRGSAALKESIITDLANKYQKSAGQVIL
                     RWHVDRGILPLPKSRNLSHQRENLAIFDFELTEEEINAINRLNKEDGRIEGQDPNEYE
                     EFE"
     gene            complement(7217..8044)
                     /locus_tag="ACHALG_002323"
     CDS             complement(7217..8044)
                     /locus_tag="ACHALG_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HFN2587684.1"
                     /translation="MFLHQKELQFEAKPDKPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMKKDPAIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDLLSVLLARDSYHQNLWAQAVKELEEKEGTILVPSTFPRNEERLDIAY
                     DFYNFSEGDQSKEGSWGKGKALDGEGEYQWLSTPKVEGKAPKLDPVTPKMYGTPPEK"
     gene            8236..9183
                     /locus_tag="ACHALG_002324"
     CDS             8236..9183
                     /locus_tag="ACHALG_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005470715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFN2587685.1"
                     /translation="MYSYYKIEKNQIRESSEEDYNWLVVKHGNEEEKKELLTLFDLPE
                     DIFIGSDEPEEVSRLEHLRDTKLNNPFLYTLIDLSSNHQERIEKRLEPVTFILSKGLM
                     ITYVSRESNLVERLIEKHGSELRHFEDIIAFATLMIYTHYINGLKEIKKQIDQLDAAA
                     RKTTENNELFNLADTERDMVYLDHTLKDQNLTVNHLMERKEFLDKLDNESLVYDIKLR
                     QKFANKLVTIYRDLLETIGGLFSDMMDNNLNHLMKYLDSAALVVAVPSLIAGLWRINT
                     GGLPGKDNTIGTILVFGLAVLVGILTAVYLSKKDFSKTK"
     gene            9818..10066
                     /locus_tag="ACHALG_002325"
     CDS             9818..10066
                     /locus_tag="ACHALG_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005225422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFN2587686.1"
                     /translation="MHWLWVLIVGAIIGLIAGAITNKGGSMGWIANIVAGLVGSAIGQ
                     ALLGSWGPQLAGMALIPSIIGAIIVVAVVSFFLGRSRG"
     gene            10266..10670
                     /locus_tag="ACHALG_002326"
     CDS             10266..10670
                     /locus_tag="ACHALG_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFN2587687.1"
                     /translation="MKKMYETAMINRGGRDGEVEAPNGSMHMKIDRPGIHSEGTNPEQ
                     LFAAGYASCFNGAVQHMLEENNLESDSEVKARVSLFQLEDGGYQIGVVLEVSLPGLEK
                     DQAEKIAEEAHAFCPYSKATRGNIDVEVTIVE"
     gene            10803..11846
                     /locus_tag="ACHALG_002327"
     CDS             10803..11846
                     /locus_tag="ACHALG_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587688.1"
                     /translation="MTQRVAIMILVLLVIGLLVYYVLKFKHWKQQRIHQDIEKKLKRY
                     PIVQAAWEKAEAKQYNIPGLTETRMVVPETGENEVCQWMTPQGLAFSQDFVFISAYCY
                     DHQHHSIIHVLDRETGQPIKLLILPKRPHVGGLVYDTKRELLWLTITGSATGRVAALR
                     LIDILADTSEETGQPIAYWLTTDLSEIPQASYLTQNNDQLVSGNFTLKGEGQLTFYLL
                     PTIAEMKTAIRRKDKIQPTVTMSRKTSDKIQGVAFYGHYLLASRSYGPYTSELLVSER
                     DSPSRILKRIKFPPYLEQIVVVEDRLAVLFESGAAAYREKANPVLANVLLLDLATLLH
                     ANKRPKKIAATKK"
     gene            11861..12424
                     /locus_tag="ACHALG_002328"
     CDS             11861..12424
                     /locus_tag="ACHALG_002328"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFN2587689.1"
                     /translation="MITVVHKERQEKSSSDVRLWVYLGSISTKIQYGYTDSAKKQGNV
                     KFLRITDIQEGRVNWSSVPYCDISNSKLIDLRLEENDILIARTGGTMGKSFLVKEISE
                     ESVFASYLIRIRLVEKLLSEYVDCFLDSPLYWKLLEKISYGTGQPNVNGTNLSKLLIP
                     LPPLEEQQRMTTKIEMIRRSIRRINFE"
BASE COUNT         3846 a         2343 c         2725 g         3810 t
ORIGIN      
        1 gaatacaatt taactcaatt tatatagctt aagctttttt ctttatttag acaaaagaaa
       61 cacataaaaa agagcagatc atccaagatg atctgctcaa aatgcggaag atgggagttg
      121 aacccacacg agatatactc acacgcgcct gaagcgtgcg cgtctgccat tccgccactt
      181 ccgcgcataa ataaccttag cgcattttga gaattttggc aagaaaaaaa ataagtctgt
      241 cagcggggac tgacagacaa ggagtaaaaa tgaaaaagtt gggttgtttg ttgggttatt
      301 tatatagtaa ccggaaaata tgaaaaaatt gtgatcaacc cttagcgttt taaagaaaaa
      361 aacaggccaa aggttaaaaa aaactaaaat agcaccatga aaaaaaaggg cctgccagcg
      421 gggactggca ggcaaggagt aaaatgaaaa agttagggtt tgttggtatg aataaactat
      481 accgcccaaa tgtgaaaaaa gagtgactaa aaaggaacgt ttttgttatg atccgtagac
      541 caaatcaagg tttttttcat tgaaggtgtc tccatgctcc aaatcaccat atttgaaccc
      601 ccggttgaac cattcaactc gttgttttga agtcccatga gtaaaactat ctggtacgac
      661 atatccttgg tattcctttt gtaacgtatc atcaccaatg gcattagcag ctgtgatagc
      721 ctcttcgata tcaccagctt ctaaaatcgg ttggccgtta taagtttttc cttgtagata
      781 cttggcaaat gtaccggcta aataatccgc ttgtagttcc aaacgaacac tatattgatt
      841 gtattctttt tcagaaacct gttggcggat cttatcgagt tgctgtgtaa taccaagttc
      901 gttttgtacg tgatgaccaa cttcatgagc aatgacatag gccatggcaa aatctccgct
      961 ggccccatac ttttgagaaa gttctgttgc aaagctgaga tccagataaa cattttgatc
     1021 ggcagggcaa taaaagggac caacttgaga agttgcattc ccacaagccg attgaacact
     1081 tcctgtaaat aaaaccagtt taggatcttg atacgtagtc tgttctgctt caaaaacacc
     1141 attccaataa tcttccaaat ggccgaagac gacagcggag aattcttttt cctctgtata
     1201 ctcgtctgaa gccacatttt ctgtttgata attagcggct tgtccgcctc ctaaaatatc
     1261 tgtgacggag tttaaatcac ccccactgaa tagaaaaaag aggactgcga taacaatcgt
     1321 accgatacca ccgccggcca ttatttttcc tcctgaacct gcggaacgac cagagcggtc
     1381 ttccacattg gaactttgtc tacccccacg ccatttcatt ttgtttcctc ctcatttgac
     1441 tgtttacatt tattataaga gaaaacgagg aagatgagaa aagtttggct tgcttgaagc
     1501 gattttgcgg aacaaacgac cgatattgct atcaagggaa agggtgtgca caccttttaa
     1561 tcatttttta atctaacaat tggtcttcat atttgttaag gtatcactag aataaaggta
     1621 gagggtgagc aaatgaaaaa caattattct ttgatcgaag accgacgcat gcaaattttt
     1681 aagcggctga tcaatgggga gcacttgagt taccagcaac tttctgatga gtattatgtt
     1741 tctaggagca gtatcgccaa ggatatcgct tatctaaaaa cactgtttgt caaagaaaat
     1801 ttactgttgc gttttgataa ttcaggcact tattttcaag gaagcgagtc acaaatccaa
     1861 cgaatgttga agcgattcat tctccttact gtggagcatg gggagaacct ttctgttctg
     1921 acaaatgtgt cattatttga aaagataaat gcggcttttc gccattcgat caccgaaaag
     1981 caaatggaga tcccagaaag ctacatccaa agcatcgttg tttcgatctt gctgattgtc
     2041 gagcgctcga atcaggcaaa tcagctggat atagaagaga agcgccaatt tggaaaattg
     2101 tttttggaat ttgacaagta tccgttgatt tatgaattgt tgaaagaaat tgaagaacaa
     2161 gaaatctacc atttttcatc gaaagaagtt cagtacctaa cctatctcat tgtaggtagt
     2221 ggcttacgtt tttttatgaa gaacgaatcg attccgttct cttttcgagg gaaagtcaga
     2281 aatctgattc agaaagtcag tgaaggcatc cagatcgatt tgacacagga tagtcgctta
     2341 gaagaagatt tgacgattca tttgtaccag ctgtttttac gcattgaggc ccaaactact
     2401 gtagtcaatc cattgctaga tgagatcaaa cagcattatc cggcgttgta tggggtcgtt
     2461 tggttctctt tggacgactt tctaaaggcc taccatattg gaatatctga ggatgaaatc
     2521 gggtttgttt cgattcactt tcaagctgca gtagaacgga tgcggcaatt gaataaaatc
     2581 gtctttgttt gtccgaacgg tgttggcacc agttcattta tttctgccaa gattcgtcga
     2641 atattgccta atgtcgatag tattgaaacc gcttctgtcg ctaagctcaa agagatggat
     2701 atttctgcca tcgatttcat tatttccacg atcgatttac ctgatgtttc caaaccagtc
     2761 gtccgcatct cgccgatggt gaccaatcga gacatgaagc ggatcatgaa tcactatatc
     2821 gatttgatta ttgataatga accgattgcg gataaggtga aaatcctgca agatacacgg
     2881 atgttgattg caccgaagat ttattttgaa cgatttgaga ccaaagaaca agcactggat
     2941 tttctgatca gtcaaaatga cttttcgtcg gaaaatcagc gtacggactt tcagcgttcc
     3001 gtcttagaac gagaagcatt gcagtcgacc tatttagata acggttttgc catacctcat
     3061 ggcaatcctg agtttgtttc aaaaacgacc ttttctgtat tgattttgga tcgtcctgtt
     3121 gattggggca accaaaaagt cgatatcatc gtgttactaa tgatacgaga agatgaggcc
     3181 caagaagtag aacctgtcat gaagttgatc atgcaaggga tcgaagacaa agaatggttc
     3241 atttcaaaaa tgttggaggt aaaatcatga tcaatttaaa aaaattactg cctgaggatc
     3301 atatcgagac cacgaatcaa atcctcactt ggcaagaagc agttcaattg gcgagtcagc
     3361 cgctattaaa tgaaaaagcc atcgatcaac aatatgtcgc taatatgatc catagcgttg
     3421 aggagaatgg cccgtatatg gtattggctg attactttgc attgatgcat gctcgtccgg
     3481 gggaaggtgt ctttcatcaa gggatgagtt tgctagtgac taaaaacgaa atcgatttag
     3541 ctggcaagtc ggtccgtatt ttccttgttt tagctgcaaa agacaatcaa agtcatttgg
     3601 aaagtttgca ggaaatcatg gaagtgttta tggatcagga aaagtatcaa cttattttga
     3661 atggtgacaa agaacagatt tgccaattat tccaatagga ggaaaagaag atgaaaattt
     3721 tagctgtgtg tggatttggt gttggaagtt caatggtgtt aaagatgacg atcgataagg
     3781 tgattaagga gctggggctg aaggcgactg ctgagaatac ggatttgtct actgcaaaag
     3841 caactcctgc agatgtttat tttacaagcc acgaattatt gaatgatttg caagcttccg
     3901 tcaaagcacc tgtctatccc atcaaaaaat atatggatgt cgctgaagtc aaaatgcaaa
     3961 tggaaaagtt tttagctgaa agagagggat aatcatgagt tttgtcacag atcatatttt
     4021 aaagaatccg ccagtattgc tcggactgat tgcaatgatc gggttgatcg tgcaacgaaa
     4081 accttttgcc gaagtggtga aaggctcgtt gacagcagcc tttgggatgg tagcattgac
     4141 cgcaggggtc aatatgctga cgggaacgat cgcaccaatc aacacagcgg ttcagacaca
     4201 gttgggggtt caagtagccg atggtctttc tgatgtgacc ttcacagcag aatacggggg
     4261 aactgttggg ttagctatgt tttttggatt gatcttgcac ttattgattg ctcgttttac
     4321 accagttaag acgattttct tgacgggtca tatgttatgg tggttcccat ttgtgatcgt
     4381 tgctggtggg gttgaaggtg gaatgtcggg gattccatta ttgattttag gagctgtttt
     4441 atctgcttgt tattggtcat ttatgccttg gatcatgcgg aaatatgttt gggatgccac
     4501 tggcgatgat tcgttcttga ttggacatcc tacagggatc ttatcgatgg tgtccggctt
     4561 cgtggcaaaa cgagtaggaa acaaagaaaa atcgacagaa gatttaaaag tgccagaaaa
     4621 cctgtccttt ttccgtgaga tttccattac aggtgccttg gtgatgtttt tgatgaatat
     4681 tgttgtagga attatcgcac cagttcttgt cccagaaggt gggaatctgg tcatgtttgc
     4741 tgttcaagca ggcttgaact ttggcgcagg actcttgatc atgctttatg gggtccgcct
     4801 attgatcaac caaattattc cagctttcca agggattgct gaaaaagtcg tccctggagc
     4861 caaaccagct tttgatgtac cgatcctatt caattatcgt ccgaatgccg tgattattgg
     4921 ttttatcgta gctatgatca cctcaacgat ccttgtgatc attgtcaata cgacgaatgt
     4981 ttttggaatc ttgatcgtac cattagttat caccagtttc tttgaatgtg gcggcgcagc
     5041 agttattgga gaaggacaag gcggcttgcg tggcgcagtc attggtacca ttgccgcatc
     5101 atttgtgatg gtcttgttag ttggtgtatc tgccgttcta ttcagcacaa cgattcaaaa
     5161 ctggattctg atctttggtg gcaatgacat atcactttgg ggaatcattg gtcgtggtct
     5221 cggcagtcta tttgggggaa tttaatatgt atcaatatgt tgataaaatt catgcattac
     5281 tagaaaccgt tgaaaaaaag gaacagcatg caatcgatga ggcaattcgt ctcttgacta
     5341 cagcaaatct agaaaagcat tcgatttata tttttggggc tagtcatgcg gggatcttgg
     5401 cagaagaaat gtactatcgc gcgggaggga tgatgacgat caatgcgatc tttggacgag
     5461 aactgatgtt ggatcgcagt cccatcactt ttactagcaa aatggaacga ctagaaggct
     5521 acggcaccgc attagcggaa atccagccgt tcaaagaagg agacgtgctg attttacact
     5581 cagtttcagg tcgtaatcct gtcataatcg atttagcact agcggcaaaa gaaaaaggcg
     5641 tcaaaattat cgctttaaca aacaaaacgt attcgcaatc agtcactagc cgccattctt
     5701 cgggccttcg tctgtttgaa gtcgctgatc ttgtaatcga taatcatgga gaaattggtg
     5761 atgcaggttg tcagattaag gggatggatc aaaaagtggg gcctacttca acggtgattg
     5821 gcgctacgat ccttaatacc attatcgtcg aagtttgcca acaattgaaa caagcaggaa
     5881 tcgattatcc gcccatcttt tattccgcaa atatggatgg cggcgatgaa ttgaaccaat
     5941 cattgtttga acaatacaaa gaagccattc attatcagtt gtaacgctga ttgtacagaa
     6001 gttttaactc agaaaaagaa ctgtgagaaa agcataatcc aaatgaaaac tgatcgtgtc
     6061 agaagttttt acttctcata aaatagcagc aagacttcag tagagccttg ctgctagtag
     6121 atctaaggaa ttcaattggg gcaagtcatc tcgcagttct ttttttgtga ccattccaat
     6181 tcctataatt gatgtgagcc tatgaaatcc ctcaggagct ttatgctgtc tcactttact
     6241 caaattcttc atattcattc ggatcttgac cttcaatacg tccatcttct ttgttcaagc
     6301 gattgattgc attgatttct tcttcagtca attcaaagtc aaaaatagct aagttttctc
     6361 gctgatggct caggtttcgg gattttggta gtggtagaat cccgcgatcg acgtgccagc
     6421 gtaagatgac ttgacccgca gatttttgat atttatttgc tagatcagtg atgatggatt
     6481 ctttgagcgc cgcgctgccg cgacctaatg gactccatgc ttcagtaatg atcccacgtt
     6541 ttctgttttc tgctaccatt tgttcttgga cccagtaagg atggatctcg atttgattga
     6601 tggctggtgt gacaccagtt gcctcaataa tggcatctaa atgttcaggt tcaaagttag
     6661 atacaccaat agaacgaatc aaacctaatt tttgcgcagt cactaacgcc tgccacgctt
     6721 caatgtactt gccgcgttta ggcagtggcc agtggatcaa aaaggcgtca aagtaatcta
     6781 atcccatgcg cgccagcgat tcttgaatac tggtcagtgc atcatcatat ccatgatatt
     6841 ttcctggtaa ttttgaggtg atataaaact cagaacgagg gacaccggat cggcgaatcg
     6901 ctttaccgac gattccttca ttgtcatagt tcgttgacga atcaatcaaa cgatagccat
     6961 ctgcaatcgc tgcttgcaac tggcttaagc cttcgccacc gcgaatttga taggtaccga
     7021 tgcccacggc tgggcgcata ttgccgtcat ttagtgtaag taaaggtgct ttcatataat
     7081 gattcctcct ttttcttata cattctactc taacgctttt ttagctttaa ggtaaccaac
     7141 ttgctcaata agacaagaaa agagcaatga tttcttgtga atggaatcat tgctcttttt
     7201 attcgtttcg atcaatttat ttttcaggtg gcgtgccata catttttggt gtgacaggat
     7261 ctagttttgg cgctttgcct tcaactttag gtgtagaaag ccattggtat tcaccttcac
     7321 catcaagtgc ttttcctttt ccccaagagc cttctttact ttgatctcct tcagagaaat
     7381 tgtaaaagtc ataggcgatg tctaaccgtt cttcattacg agggaaagtg ctcggtacta
     7441 agattgtacc ctctttttct tcaagttctt tgacagcttg tgcccaaagg ttttggtgat
     7501 agctgtcccg agctaatagc acggataaga ggtctttaac tccacggtca tcaatcattt
     7561 catagattcg agcgacttgt aatcgtcctt gtgattctgc atttaaattg gcacggaagt
     7621 ctgccagtaa attaccgctg gcaatgatat aagcgccact ccatggatta ccgacactgt
     7681 cagctggacg tggtcctaag ccagcaacga tcgcatgttg tggattcatt ccagagataa
     7741 ttgcgccaat tgctgggtct tttttcattg cggcttcttg atcttctacc ggtgcacctt
     7801 ctaataaacg agcaaccatt gtagcaagca tttcaacgtg tccaatttct tctgtcgcaa
     7861 tgtccatcaa catatctttg tacttttctt ctccacggca gctccaacct tggaagagat
     7921 actgcattgc tacactcatt tcgccatact ggccaccgat caattcttga agatacttcg
     7981 caattaatgg atttggtttg tctggttttg cttcgaactg tagttctttc tgatgtagaa
     8041 acatagttgt cctcctctaa aatgattttt attgggccca aaaatattct ctcacgttta
     8101 tttagaatgg tctgatcaaa cgcttgcttt atttccgtat taataaaaac cgttgaaata
     8161 gcgctttttc ttgacaaaaa gcagtcgcca ttagatagtt gaagagaagt gaaggagtta
     8221 aaggagagag gcaaaatgta ttcgtactat aaaatcgaaa agaaccaaat cagagaatca
     8281 tccgaagaag actataattg gttggtagtc aaacatggaa atgaagaaga aaaaaaagag
     8341 ctgctgacac tttttgattt accggaagat atttttattg gctccgatga accagaggaa
     8401 gtttctcgat tagaacattt acgagacact aaattaaaca atccgtttct ttatacattg
     8461 atcgatcttt cttcaaatca tcaagaacgc attgaaaaac gccttgaacc tgtcacattt
     8521 attctttcca aaggattgat gatcacctat gtctctagag aatcgaatct cgttgagcga
     8581 ttgatcgaga aacatggaag tgagcttcga cattttgagg atatcattgc ttttgccaca
     8641 ttaatgatct atacccacta tatcaatggg ttgaaagaga ttaaaaagca aatcgaccaa
     8701 ttggatgcgg ctgcccgtaa aacaaccgaa aataatgaac tatttaatct ggcagacaca
     8761 gaacgagata tggtttattt ggatcacacg ttgaaagatc agaatcttac agtgaatcac
     8821 ttgatggaac gaaaagagtt tttagacaaa ttagataatg aatcgttagt ttacgacatt
     8881 aaactgcgac agaaatttgc caacaagctt gtcacgatct atcgcgattt attggaaacc
     8941 atcggtggtc tattctcgga tatgatggac aataatttga accatttgat gaaatattta
     9001 gattcagcag cattagtggt tgcagtgcca tcattgattg ctggactttg gaggatcaac
     9061 actggcggac tgcctgggaa ggataacacg ataggaacga ttctagtttt tgggttagct
     9121 gtcttagtag gcatccttac tgctgtctat ctttcaaaga aagacttttc aaaaacaaaa
     9181 taaaaaagca actttgtcta gcattgaacg ctgggcaaag ttgctttttt tcgtgacttc
     9241 acaatcgcga agactgtcca ataagcagag gggagaagcg agaaatgaaa aatattaaga
     9301 agaaaggccg gggtacaatc cgaacaattt atattttact ttactcgcta ttgcatacgc
     9361 tcacttctat cgttgctttt tctcataatc cttttacata gtgaggggaa gcaggtttca
     9421 aatacaaaag gcagaatcaa aggaatgcca attgttttga tgtttcatca gaaggatgta
     9481 gtgaatgagg tgtgtacgac aatcaggttt ctttgattgc ttttattcat agggaagatg
     9541 acggttgaaa gcagacaatg aaagaaaatc gtacatagtc taggtaccct ttatcagaaa
     9601 ataacttttt gctgatcgaa acgctttcaa agagctgcgg cagttttttt gacctcttgc
     9661 agtgagtgtt aacgattcca aaaaagttga cttatcctta atataacaac gttttaaatt
     9721 ttttttgttt ttttaagagc aaaatttttg tgtattttta agaagaagcg taacataata
     9781 tgtgtaataa atatttcaca gaaagaaaga gaaaattatg cattggttat gggtattaat
     9841 cgtaggagct attatcggtt tgattgccgg agcaattaca aataaaggtg gatcaatggg
     9901 atggatcgct aacatcgttg caggtttagt tggttcagca attggacaag cattgttagg
     9961 tagctggggt cctcaattag caggaatggc tttgattcct tctatcattg gtgcgatcat
    10021 cgttgttgct gtagtatcat ttttcttggg acgttcaaga ggttaatcgt caaatatatc
    10081 agcaatttta cctattatct taaggagcag cttttcgctg cttctttttt tgtatagata
    10141 aactgcttct atcactgatc atcgatgttg tctaactgat gtcgccgaat gcaagcatta
    10201 cctttgtcag attaaaaaat actcgttaca cttaatatag aacgacgaaa gaaaggatga
    10261 tctcaatgaa aaaaatgtac gaaacagcaa tgatcaatcg tggaggccgt gatggcgagg
    10321 ttgaggcacc aaacggaagt atgcacatga agatcgaccg cccaggaatc catagtgaag
    10381 ggacaaatcc ggaacaactt tttgcggctg gatacgcatc ttgcttcaac ggcgcggttc
    10441 aacacatgct ggaagaaaat aatcttgagt ctgattctga agtcaaagca cgggtatctt
    10501 tatttcaatt agaagatggg ggctaccaaa tcggggttgt tttagaagtt tctttgccag
    10561 gacttgagaa ggaccaagca gagaaaatcg cagaagaagc ccacgcattt tgcccatact
    10621 ctaaagcaac aagaggcaat attgatgtgg aagtgactat agtagaataa gggattctta
    10681 atgttactat aaatgagatt atgaaagatc ctataaagaa acgctagtaa aagaaacatg
    10741 tagttacggt aattaatagc ggtatatgta cgattgatcg aatagaacaa ggaggcaaaa
    10801 aaatgacaca gcgggtagca ataatgattt tagtgttgct ggtcattggc ctccttgttt
    10861 actatgtcct aaagtttaag cattggaagc agcaacggat acatcaggat attgaaaaaa
    10921 agttaaaacg gtatcctatt gttcaagcag cttgggaaaa ggctgaagca aaacaatata
    10981 acattcctgg attaacagaa acaagaatgg tggtgccgga aaccggcgaa aatgaggttt
    11041 gtcaatggat gacgccgcaa gggttggctt tttctcaaga ttttgtgttt atttctgcct
    11101 actgctatga ccatcagcat cattctatta ttcatgtact ggatagagaa acaggacagc
    11161 cgatcaaact gctgatttta cctaaacggc cacatgtagg gggcttagta tatgatacaa
    11221 aacgggaact tttatggttg acaatcacag gatctgctac tggacgagta gccgctttac
    11281 gtttgatcga tatcttagca gatacatctg aagagacggg acagccaatc gcttattggc
    11341 tgacaactga tctctcagag attccacaag catcgtattt gactcaaaac aatgatcaac
    11401 tggtatctgg caacttcaca ctaaaaggtg aaggacagtt gactttctat ctattgccga
    11461 cgatagctga aatgaaaaca gcaattcgcc gcaaagacaa aatccaacca accgtgacaa
    11521 tgagcagaaa aacatctgat aagattcaag gtgtggcttt ttatggccat tatctcttag
    11581 ccagtcgttc ttatggtcct tatacctcgg aactcttagt gtccgagcgg gactctccta
    11641 gccggatcct aaaacgaatc aaatttccac cgtatttgga acaaatcgtt gttgtagaag
    11701 atcgtctagc agtattgttt gaaagtggcg ccgcagcgta tcgcgagaaa gccaatcctg
    11761 tgttagccaa tgttttatta ctagacttag caacgttgct tcatgcaaat aagcgtccca
    11821 aaaagattgc tgcaactaaa aaatagtcgg acaactcaag atgatcactg ttgttcataa
    11881 agaacgacaa gaaaaatcat ctagcgatgt tcgactatgg gtttatttag gaagtatttc
    11941 tactaagatt caatatggct atacagattc ggcgaaaaaa caaggcaatg ttaagttttt
    12001 gagaatcaca gatatacaag agggacgtgt aaattggtcc agtgtgcctt actgtgatat
    12061 tagtaattct aaactaattg atttaagatt agaagaaaat gacattttga tagcaaggac
    12121 aggaggaact atgggaaaaa gtttcttggt taaggaaata agtgaggaat ctgtttttgc
    12181 ttcttacttg attcgaatta ggttagttga gaaattatta tcagaatatg tagactgctt
    12241 cttagactcg ccattatatt ggaagttatt agaaaaaatt tcttacggaa ctgggcaacc
    12301 aaatgtaaat ggtacaaatt tatctaaact acttatacca ttaccacctt tagaagaaca
    12361 acaacgcatg actactaaaa tagaaatgat aagaagatct attcgtagaa tcaattttga
    12421 gtaaatataa cggacctagc atccatttta taccagatga tgctaggtct attatttata
    12481 ttttatggaa tttaattgat ttgttctagt gttttaatat tattacgaca gtaaaaaaat
    12541 agtttgattc ttctaacatg aatgaccatc tagaagtgga aatgtataaa agatttactt
    12601 tatgaagttt ggtgttagta tcaaatctta gacagctttt cgtagagaca agaataaatt
    12661 aaacttatct acgagaggat gaatttttat gacccgatat gaagaaaatt ttaaacaaat
    12721 gatt
//
LOCUS       DAZABD010000075        12351 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000075 DAZABD010000000
VERSION     DAZABD010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12351
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.076"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     regulatory      complement(147..313)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            complement(538..744)
                     /locus_tag="ACHALG_002329"
     CDS             complement(538..744)
                     /locus_tag="ACHALG_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587690.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            complement(760..1374)
                     /locus_tag="ACHALG_002330"
     CDS             complement(760..1374)
                     /locus_tag="ACHALG_002330"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFN2587691.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(1521..2204)
                     /locus_tag="ACHALG_002331"
     CDS             complement(1521..2204)
                     /locus_tag="ACHALG_002331"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFN2587692.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(2313..2771)
                     /locus_tag="ACHALG_002332"
     CDS             complement(2313..2771)
                     /locus_tag="ACHALG_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFN2587693.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(2855..4618)
                     /locus_tag="ACHALG_002333"
     CDS             complement(2855..4618)
                     /locus_tag="ACHALG_002333"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFN2587694.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(4839..6299)
                     /locus_tag="ACHALG_002334"
     CDS             complement(4839..6299)
                     /locus_tag="ACHALG_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587695.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(6507..7433)
                     /gene="glsA"
                     /locus_tag="ACHALG_002335"
     CDS             complement(6507..7433)
                     /gene="glsA"
                     /locus_tag="ACHALG_002335"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFN2587696.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(7456..8883)
                     /locus_tag="ACHALG_002336"
     CDS             complement(7456..8883)
                     /locus_tag="ACHALG_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFN2587697.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(8914..10128)
                     /locus_tag="ACHALG_002337"
     CDS             complement(8914..10128)
                     /locus_tag="ACHALG_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFN2587698.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            complement(10214..10441)
                     /locus_tag="ACHALG_002338"
     CDS             complement(10214..10441)
                     /locus_tag="ACHALG_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587699.1"
                     /translation="MKNKFENRSDTRCGSFLYILYEVFFIPYEKISLKIKKDENKRFS
                     IKRRPFIFCLYLCEKRKRAQHIKLLLIVCLL"
     gene            complement(10469..10711)
                     /locus_tag="ACHALG_002339"
     CDS             complement(10469..10711)
                     /locus_tag="ACHALG_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFN2587700.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(10743..11300)
                     /locus_tag="ACHALG_002340"
     CDS             complement(10743..11300)
                     /locus_tag="ACHALG_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFN2587701.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(11313..11501)
                     /locus_tag="ACHALG_002341"
     CDS             complement(11313..11501)
                     /locus_tag="ACHALG_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFN2587702.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(11514..12080)
                     /gene="amaP"
                     /locus_tag="ACHALG_002342"
     CDS             complement(11514..12080)
                     /gene="amaP"
                     /locus_tag="ACHALG_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFN2587703.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
BASE COUNT         3637 a         2547 c         2015 g         4152 t
ORIGIN      
        1 ccaccaatcg atcttttttc accgcatctc ctcctcacac agcaatccac ttgtcaggtg
       61 aggagcgagt ttgtctcatc aaataagtca gtcacaaaaa tataaagaaa aggtcagaaa
      121 ccttttcgaa caagaattct gacctagcat catctcatcg tccgagtttt agcactatac
      181 aacgtaaaga aaacattctt agctattgga aaaagcctta atccaacagt ttctgtttta
      241 cccattggcg actttcgacg tttctgggca acagcctatg tttgtatagg agcctcacct
      301 aacaaggtta ttactatatt ctatttacga gttcatgata gacttttcct aatgattagg
      361 caatggaatt tgaaatgaaa tagtgaaatt taacaatctt taatattttt tagaaaaaaa
      421 taacaaataa gttgaaagaa tcccgttgca aattctttca gttgcaccag gattctttga
      481 taatttttta gttttatgtg tttttttata tgttttttca cttttcggaa taaatattta
      541 tttgttaaag taattaaaca atccgctaat cgtttgatct ggcatttcta tttccttacc
      601 atctaaagta acagtagtaa cgacaaaatg gtcttgcaat ggcggaactt ctaagtactg
      661 cccttgcaga aaatcattca ctgtttcata ttcttttttc atttcttttg aatctgtcga
      721 tggtttatag cttattttta acataactag tgctcctttt tattcgggac gtgcaatcgt
      781 gaaaatttct cctattgctg cataatcatt tcctgccaaa cggcttacag cattcaattt
      841 tcttggatcg atccttcctg tcttatcatc atagactgtt tcatttaaat gatatttctg
      901 tacttctagc aaaaataaat cagctgttac tttattttta ttccgtatct cgacatgttc
      961 atataaggat gtttcaaaac gaacttttgc ttctgtcaat gctggtactg aaatgattac
     1021 tgaatcagtt aaagtcaagt cagtcaggta cagttcgctt tcctcattag gcagattggc
     1081 tgctgtttta tttacttctt ctacgttgtc ctcatcaagg atatggataa cagcttctct
     1141 tgtctcaaga atattctttg ctgtgtcttt tactgcatct tgttttctct ggatcgccaa
     1201 ggataagatg ggtcgattcg aactgacgat attgaagtaa ctgaagggtg cgatattcac
     1261 tatatctttt gctgatttag tcgttactac tgctactggt ctagggatga ctgaaccaat
     1321 cagcaacttg taattttcac gttctgtcaa ttctgaagga ctccgttcaa acatggtttc
     1381 gccttctttc tataaaattc gttaaaaagc agatcttgaa gacaaaatga aaagtaatgc
     1441 ttttttcatc ttcttagaat ctgctttaaa ctatctatca atttttcttt tttgtcaaga
     1501 atctgtgccg tgcgtgtttt ttagttattt gctttacttg cggcttcgac atatctttta
     1561 gtgatcaatt gcggacgggt gataatagat ccaactacga ctgaaaaagc acctaattca
     1621 tatgctttct ctacttgttc aggagagtca gtatgtcctt ccataattac tgggatatcg
     1681 acttgttcta tgatttgatt caataaatca aaatcaggtg tctgcgtctt tcgtgaatat
     1741 tctgtatagc ctgctaaagt cgtagacaaa agatcaaaac catcttcttg cgcttgaagc
     1801 gcttcctcaa aagtcgaaat atctgccata gctaaccgac cgtgttcgtg aatcatttgg
     1861 acaagttcag cagttgtttt ttctgtcgga cgtttacgag aagttgcatc cagtgcaatg
     1921 atttctgctt gcgtctccaa aagttcttcc acttcttttt ctgtcgcagt aataaacact
     1981 tctgacccat cgtaatctct tttgataatc cctattaccg gtaaatcaac gattcgcttg
     2041 atttcattga tatcttctgt cgtatttgct cgaataccaa ctgctccgcc ttcttttgca
     2101 gccaaagcca ttctccccat aataaaagaa ctgtgtagcg gctcgttttc caaagcttga
     2161 caagaaacga cgactccgcc tttgatttgt tccagtgtat ccacaatatc tcctccagta
     2221 atgttatata aaaatacagc ttcatataaa aatgtaaccc ttttctattc aggagacaat
     2281 aatgagctat ttctttcagc agacctttct tactaaaaga aaattacttc aaattcttcg
     2341 tatacgacca gcttctcttc tgtaaattct atgttcaacg cttctttata agcatgttta
     2401 aaaaactcag catggacttt ccgccaataa gctaatgtcc gatccccttc tccttctttc
     2461 caagcatgct ctttcgtgac ttcactaaaa ggcaagatgg tcacatttat atttttgatg
     2521 atacaaactg gttcattttt tccattcagg accacgtcta attgaccaac ttttggtaaa
     2581 ggctcattct cttgtttata cagatcgtaa ccacttgatg taccagtctt tatctttttt
     2641 acaactagtt cgctcaactc gtctgccatt ttttctgtat ccccaaatga aaagaaacct
     2701 aatttttctg attgataact aggatatttt tcttggaact ttttgacata ttcttctact
     2761 tgctgataca taacatccac ccctttgctt tttataatga aaaaaccatc cagatactag
     2821 tataaacaat tatctggatg gtgagcataa tgaattattg aatattcaag acacgcagac
     2881 tatcttcaac gatttgtttt ggtgttaaat gataacgttc ataaagctgc cctacaggaa
     2941 cgctatccgt gaattctttc tctgcaccaa aatttaatac gttcattgaa gaatcaccat
     3001 aataacgact gatattttcg ccaaatcctc ctgacaaact accatcttct aatgtgatga
     3061 ctaattcgtg tgtttctttt aatttatcta atgtttctac gtcattttct gtgatcgttc
     3121 ctggattgat caacgttgct tcgattcctt tttttctaaa agcttttact gtttcttttc
     3181 ctaattcaag aaaaccgcct aaagctaaaa ttgctacttt tcctccatta tttactgttt
     3241 gataggctgg aatactataa tcatttgcga tagctgtacc atgttctaca ccatgtgtag
     3301 gaattcggat tgctacaggc tgcgctgttt gttcgaacgc ccaatctagc atagagatca
     3361 tatcctcttt tgttgctgga gcaagatatt tgatgttcgg gatacttgat aagtaaggaa
     3421 tgtcaaatgt tccttggtgt gttcggtcac tagctgagat cgttccgcca ccgactaaaa
     3481 taatcactgg attctcgttg atcgctaaat catgtgataa ttgatcatat gcccgttgca
     3541 agaaagtact tgattggaag acgactggac gtactccctg agaagccaaa gcagaagcga
     3601 aagtgataga gtgctgttca gcgatcccca cgtcaaaata atgatctgga tgtttttctt
     3661 ggaatgtttt cagtccaaag gcgcctggta tcgctgcatt gactgcggca ataggagctg
     3721 tacttgtttg cattttctta tccaaataat ccatgatgat catattaaat gtttctccag
     3781 aagttacttg ctttgcctcc ccggtggaca gatcaaatgg tacatgccaa tgatatttca
     3841 ttttattatc gattgccggc tgataaccgc gacctttttc cgtatggacg tgaagtacaa
     3901 tcggatgatc aatatctttt acttcttcaa ataaacggat caccgtttcc agatcgtttc
     3961 cattatccac gtaacgataa tcaaatccca ttgctttaaa taggttattg ctgcttttac
     4021 cgttggtttg acgaagctca gctaagtttt tatataaact gccatgattc tcatcaatag
     4081 acatctcatt atcgtttact aatacgatta gattggattg taaaacacca ccattgttca
     4141 atccttcaaa agctaagcca ccacttaggg agccgtcacc gataaatgct accacatttc
     4201 ctgattcttt cttcagatct ctcgctttag ctaaacctgt tgctaagctg atagatgtag
     4261 atgtgtgccc cactgtaaaa tagtcatgtt tactttcatg ttggttggaa tatccgctca
     4321 cttcacggta atgtcctttt tcaaaaccat tttttcgacc agttaaaagc ttatgaccat
     4381 aagactgatg ggaaacatcc caaacgattt tatcctttgg tgaatcaaat acataatgaa
     4441 acgcaatcgt taattctact acacctagat tcggaccaac gtgcccacct acatcgctgt
     4501 ccttttgcaa gattttttgg cgcatctctt ctgccaactg tttcatttcc tcaacagata
     4561 attttttgac atctgcgggg ctattgattg catccaatac atgtaatact gtactcataa
     4621 tacattctct cctccgtact atcttctttt aatgaaaata gatttgttac actttatcta
     4681 ttctactact tagagttgac tctaagtcaa tagcatttta aaaaaatttc agagaaagct
     4741 tgttatataa gtagatcttc gcacgaatca ggactcaaat aaaaaccttc tacagacaat
     4801 taaaaagctt tgtagaaggt ctcctaatta aggtctcatc aagaaatatg tttttttaat
     4861 aggtgaagtt cctgagcaat atgatacaaa tggatgtttg caccttttaa tgagattgga
     4921 tagatctttg tcttttcagt ttctaatcca ttttgatagc tagcagcatc gcacaaaacc
     4981 actctttttt tcgcacatag acattcatca ggcaattctt ctaatgaacg aaataaaata
     5041 ggatttattt cagcgatgcc ttcttccgca tacaaatgaa attgggttcg ataaaaaaat
     5101 tgtttgactg aactttctga aacaacaaaa atttctattt cctgtctccc cgcttgttca
     5161 ctgttgatca aagaaagact tttgacgaat tttcgaatga aattttcatt ccatcttagt
     5221 tggatttgat ttttcttttt ccactcttcc aatatttcta caatttttcc gtacagaagt
     5281 tgttcttttt ctgatagaag atagatcctt tccggtaaaa atacttgtcc atctgcccac
     5341 aacgtacgga caaattgtaa aaaggattgc ttgaaaataa aattttctgc atcgtcgatt
     5401 tcagcgatac taataatctg taaaatcaat tcattcagtg ataggtcatt gagaacaaaa
     5461 tttttataaa caacttcgaa atcttttctt aacagctcca tgttctcatt ggtataattt
     5521 ctactgttga acaaagaaaa aatataatag acatcttctt cttgtaagtc tagaattcct
     5581 ttttgctctt ccaatccttg acagactaat tgaaataacg gcttcgtttc tgcgcttttc
     5641 ttatcttctt ctttcagtcc aaggggaaat gcctttcttc cgattgcgat ctctattcct
     5701 cgtatgataa aggacttttc atcttccgaa tatcttcttt taaaaaaatg agtctctaca
     5761 taatcaatca actcgttaca agcttcttct acatacggat ttttattttc atagccttcc
     5821 caattgacat aacgtactaa agctagcaag atgttccgcg agtcaaattc tgggataatg
     5881 atttctttat ttttcaaata atcattttct ttaaaaaaat ccaatacgat ttttttgatt
     5941 gtatatactt tgctcaaaga aataaattca agatcagcca gttctgtact cgtgaatata
     6001 ccattaaaat aataacttaa aaactttaaa aaataagatt gaccgtacag aaactgtgtg
     6061 agctcagaaa gagcaatacc atgtttgaat acgagtttat acttatgctt ctctcttact
     6121 tgttctttta tacaatattc tagacactcg gctagacttt ctagatcgtg aaggatcgtt
     6181 ggtgtagtca cccttagaga aatagctgtt tgttcgatgt ctatttcttg gaattcaaat
     6241 aattgttcac acagatacac ttttctaaat atatttcgct cgatgtaatt ttccagcatt
     6301 cctgatacac gcctccagtt tttcttatga ttttatttaa tcatatctgt aaatcgtatt
     6361 tttgtttcct ttttatttag aaaagccctt tcaaaaacca ataaaaaaag aaactgggac
     6421 aaaagtcaaa caagctcaca atttcagcct cattgtaggt agctttgagc cgtttggact
     6481 tttaccccag cctctttcaa gaatggttat tcaaagatgt cagcgtctag ttctttgact
     6541 acatctttca acaattggat accagctgcg ctgttaccaa atggatctag tgccgggctg
     6601 aagacaccta tgccgtaacg atttggcaca gctgccatca acccgccgcc tacaccgctt
     6661 tttgcaggta caccaacatg gacggaaaac tctcctgatt catcatacaa accagcagtt
     6721 gtcataattg atttgacgat tgtagcactt tcttcagtaa tgatttgttt gccattccaa
     6781 ggagcaattc ctttatttgc cagcacagca gctaattttg ctatgcctgt agcggtgaca
     6841 ttgagggagc attgtctgaa ataagcatcg agtatctccg gtacgtctcc ttcgatcatt
     6901 tgattacctt tcatatagta agcaagagag cgattgatat cacctgttct tgattcagaa
     6961 tgatagattt cttcattcaa tgtgatatta ggatcatcac agatctcttt cataaatgac
     7021 aagattcgct caaatttttc atctgcatct ttaccggcaa tcaacgaggt tgttgcaatc
     7081 gctcctgcat taacaaaagg attcattgga tgtttgcgat gattgatttc catattcatg
     7141 atagaattga aggcaaaacc ggttggttcc gtgttgattg tttgaaagac tttttctatt
     7201 ccgttgtctt tgattgctaa taacaatacg ggaacttttg acatgctttc gatggcaaag
     7261 cgcacatcgg cgtctcctcc ctcaatcagt tgttttgtat ccaaatcata aatcgccata
     7321 cctaattgtt tgggatccac ttcagctaat gctggaatgt aatccgccac ttgtcctgtt
     7381 tgataatagt ggaaattttt tccaatcaat tgttctaatt cttttacttc catttgaata
     7441 accagctttc attttttatt tttctgcatg ctggaattta tgacggcatg cgtagatcgt
     7501 aaatggaata actaagacga taagataaga aacaatcaga atcgtttcat aaatcccatg
     7561 ctgtccagct gcgatactag caggtggaac aaatgagata caaaaagcaa aaagtgaagt
     7621 caaaagacct aaacaagcaa cgattacttt tacaactttt ccgcctggaa cctgataggc
     7681 tctttcaaga ctgtcatctt taaaaaccag cttcaaatag cccgcgaaaa acataaagta
     7741 caacatcagg tagattacaa cagtcaatga catcgctgca agaaatgaaa gattattccc
     7801 cccgccgctt aatgtaaaga cggctgccca gattgttgca acgatcccat ttgcgatcat
     7861 caaatgagca ggaacaccgt gtgtgttttc ttttgcaaaa tattcaggaa gtaatccttc
     7921 ttctgctgct acgtgaagtg cttttgaagg agaggttacc caagaagcaa tttctccgat
     7981 gacacctaaa caaacaagaa tcgcaagaat tttcgcaatc catgcaagat ttggatcaat
     8041 gtgtcttaat aggaattcta acgattgaat cacccctgta ttaagagaca aaccatgttg
     8101 aggtactgta gtagcgacgg tcaatccgcc aaaagtatct aaaatgattg ccagaataac
     8161 caatacaaac attgctaatg gataatcctt tttagggttt tccatttcat ttgcatgact
     8221 ggcagatgtt tctacgccca tatatgctaa aataaaggaa acaaaaacaa ccagcgtagg
     8281 caatttcgaa aaatcaggaa tcaatgtttt catagacagt atcgtttgta gtggagcgcc
     8341 tgaagcgaca taatggatcc ctaagccaaa caagactaat gctggcaaca agataccagc
     8401 tacaaaaccg atccgagcaa tcaatgaagt gtatttggtt ccttttaact gtgagagtaa
     8461 aatagcccag aaaataatca ataggattgc caatttcaga tatttattgt tttgtatagc
     8521 accaataccc gttgcatagg ataatgcacc agttaagaaa tacagcatgg tcaagtagcc
     8581 aactgttatc tcaaaccatt ggaaaaagat tgcggcaaaa ccgaatcgtt ttcccaatgt
     8641 gcgactgacc caagtataga caccgccttt ttcccatcct ttcaccgttg ccatctctgc
     8701 tgcgcacaat gccacaggga taaaccatag caaaccgcct aataacaaga aaaatactaa
     8761 gctgaagcct gaggtcgcga atgtgggata ttcatataat gaaacgacca ttgagatggt
     8821 catagccaaa aagccaaaga acgttaattt ttttgcagga attttcgttt tgctttgttg
     8881 catgaatctt cctcttttct atttgaattt gtttcaattg agtttgttgc caaagaagaa
     8941 aaagtccaat ccttcttctt gttcttcttt tgtagtagtg atcgttgtaa agtgcatcgc
     9001 cgattttaaa atcactgttg tcagcacata ggcatacacg gcagtaatga tgacaccaat
     9061 cacttgaatc cctaagaagt accagctgct tggattcgtt agtaaatggc tgttagatgc
     9121 aaagatccct atcagaatcg tacctgtgaa tccgcccatg ccatgaacac cccacacatc
     9181 aagtgcatca tcccattgtt tctttttacg aaaatcaaca gcagcatgac agacgatacc
     9241 cgcaatgatc ccaatcagca tggcactttt tgcttctaca taaccggcac aaggcgtaat
     9301 ggctgccaat ccagcaacgg atcctgttag tacgtcgaca aaatcaaagc catttccttt
     9361 tacttttgca atgatcagcc aagtaatcat cgcaaatgca agagcgatga atgttgaacc
     9421 aaatgcattg actgcttgtt catctgctcg aagtgcacca ccggaattaa aaccgaacca
     9481 gccaaaccaa agcagtcctg taccgatagc agctgccata agactgctat gctgacttct
     9541 ttctcctaaa ggaatctttc tttgaccaag tgtgaggata catgctaaag agccgaatcc
     9601 agctgtcgtg tggatgactg ctccaccagc gaaatctcta aatcccatct gatccaaaaa
     9661 tccgccaccc catatccagt ggcaaacagg gaaataaatc agcagattcc aaaaaataac
     9721 aagtataata tagcctttta aattcaatct ccctgcaaaa gctcctgtca tcaaaggaac
     9781 ggtaatcacg ataaacatca gttggtaaag aaagaacatt aaaaatggaa tcgttgcacc
     9841 atgaaacatg ttggggaagc ttgtaacatg tctcatcaaa aagaaatctg ccgggtttcc
     9901 aataacaccg ccgatatccc gtccaaaggc caatccgaat ccaccaaata cccacataat
     9961 cgttgttatc ccaacggaga gaaaagattg agacatgatc gttagtatat tattcttccg
    10021 tgctaatccg ccgtaaaaaa aagctagacc aggagtcatc aggcaaacca ttgctgtaca
    10081 taaaatcatg aatgctgtat ctcctgtatt gataggtaat gtattcattt ttttcgcctc
    10141 ctttctacga ttacgagtat aaaagaaggt gtagttgttt ttttcttctg tttttttgaa
    10201 ttaatacttg catttataaa agacagacaa ttaataaaag cttgatgtgt tgtgctcttt
    10261 ttcgtttttc acacaaataa agacaaaaaa taaacggtct tctttttata gaaaaccgtt
    10321 tattttcgtc tttttttatt tttaaagata ttttttcata aggaataaag aatacttcat
    10381 ataaaatata taaaaaagaa ccgcagcgag tgtcgctacg gttctcaaat ttgttcttca
    10441 tttcaacata agtttttctt caatttgctt ataatttacg tgcaaagaat gaaacgacta
    10501 atactaaaat gattgcaccg atcaatgatg gaatgatagc cattcctgca attgttggtc
    10561 cccaagttcc tagtaaagct tgtccaattg ctgaacctac taaaccagca atgatgtttg
    10621 cgatgatccc cattgatgat cctttattcg tgattgctcc tgcaattgct ccaatgatac
    10681 cgccgacgat taatgcccaa ataaatccca ttttaaattc ctcctaactg acttcttact
    10741 tcttattgta cacgtggttc gttattttct ttgacttttt gtgcaccttt gcctacagca
    10801 gattttgctt tatctgtttg gcgtgaagca aattgtcctg tggattttgc tgcttctgtt
    10861 actttgtctt gtactgtttc ttgatctttt tggtattctt cttgtgtttt gatgtctgca
    10921 acattcacat tcacttcgat aacatctaaa tgtgtcatat tgtttacttc agtactgatc
    10981 aattctttga tttggttgta aatatcttcg atgtctttac cgtattctac gatcacatct
    11041 agatccacag ctacttgttt cttacctact tctgtattga tacctgctgt tacgttatct
    11101 gtgttgacta atttttcagc catatttgag aaaaatccgc catctaatgt caatagacca
    11161 tctacatctt tcaatgcata accgatgatt ttttggatga ctttgtcttc ataagtcaat
    11221 tcaccattca tcatgcggtt ttctcctttt tcatttgcca aaactgatcc atttgttgta
    11281 ttcatgtttt tgttttccat ctttgtttcc tcctatgatt taaaaaattg atcaactatt
    11341 cctgactgtt tcaaataaaa accgattcct gcgccaattg ctgtcatgat tatgatcaac
    11401 aatgtcttga aaaatccaat cgttaaccat aaaacagcta gcagtagtcc aagaccccca
    11461 aagataatag ggagcttgta tgctgaaaat agttctttca ttatttggcc ctcctattcc
    11521 actcgagagt gcttgtagtc tcttgtatct tcttgttctt cataaccgct gtatgtgact
    11581 gccactttga ccgtttcttt tgttccgagt actttagtta cttcttcttc gatatcttgc
    11641 atcaatacac ctgttttccc tactaatgaa gaagttcgtt tcaagtctcc gttgacatgg
    11701 atatggattc gatttttcgt tgaacgtatc cgtactttag gtgaatgaat gaactcttct
    11761 tcgtgtaaat gggaacgtac caacccttcg atcgcttttt tgtcgattgt tagtttgcca
    11821 tcttcccgtt tcataacgaa agttcctctt gactttggat agaaaagaac gactagcaag
    11881 atgataatag caagaacggc aaatactaca gctgcccaga aaatatattg ttgcatgtag
    11941 taattcgtta aagtaaagaa gcgaaatcgt tccaattgtt caggcataat atacggcgca
    12001 ttgatcaacg caatcgctag aaatactgaa agtagcagta aactcatgat aacaccgatt
    12061 atttttaacc ccttattcat tttccacctc ctataaactt atttttaccc tgtaaataac
    12121 tctaccgaca atcacagtat agatagcgaa cagttagcat agcatcagta aataaagcgc
    12181 taccaaacat tgctggatca taacggcgtt ttattgccag tctgatactg aaataacaat
    12241 tgcttactta ttctctattt acttttgatt gctggctata cttaaaggat attattcaag
    12301 attaaaaatt gcaaagatta tgctcagtta ttattacaag attctattta t
//
LOCUS       DAZABD010000076        12340 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000076 DAZABD010000000
VERSION     DAZABD010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12340
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.077"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..154
                     /locus_tag="ACHALG_002343"
     CDS             <1..154
                     /locus_tag="ACHALG_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HFN2587704.1"
                     /translation="SLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQRRFEGTL
                     RYSLGY"
     gene            complement(208..486)
                     /locus_tag="ACHALG_002344"
     CDS             complement(208..486)
                     /locus_tag="ACHALG_002344"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587705.1"
                     /translation="MENKVGYVRYNFFSSTPIMKDFASFNEALAVELSHDRERIHYEK
                     HVIINDLWNTEKTDLLALPDTDYPVFKEIEVKANKYNEIKQNKITNLS"
     gene            complement(601..1218)
                     /locus_tag="ACHALG_002345"
     CDS             complement(601..1218)
                     /locus_tag="ACHALG_002345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587706.1"
                     /translation="MLTMTDINTIRNLRNNHDKSINHIAKELDVNWRTAKKYADDDLL
                     PQPKARKKTGMMYTEKWGGIVSLWLSEDAKLPNKKRRTAEAMTTALKADGFPGSYRTV
                     CVFIQDWKATHYLETLEDQGFERLEHPPAEAQLDFGTMEVCHDGAFKDVKVLVMTFPF
                     SNAGFAVVLPAENQECLLEGMKELFSQSGGVPRKIRIDNMSTAVT"
     gene            complement(1613..2437)
                     /locus_tag="ACHALG_002346"
     CDS             complement(1613..2437)
                     /locus_tag="ACHALG_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFN2587707.1"
                     /translation="MEIKVLFENSRINLNFKSGHGLSLLINYSGKKYLFDAGANKKLV
                     YNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLKILTTFL
                     YVGIPKNEILKMHENLHTIKTKTELAPSFHIIPLGKANSTTKNLYKKMDDKFVYDDFS
                     DELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMIGIPYINNL
                     GLPEQEVMKIGNKLNDSRVEKILTCHCTGSKAFNILKTQLGNKLEAIKTGQHLEIS"
     gene            2780..3196
                     /locus_tag="ACHALG_002347"
     CDS             2780..3196
                     /locus_tag="ACHALG_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFN2587708.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIKESQQSVIDCRKLLVEQLTGGDDL"
     gene            3193..3663
                     /locus_tag="ACHALG_002348"
     CDS             3193..3663
                     /locus_tag="ACHALG_002348"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFN2587709.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            3677..4450
                     /locus_tag="ACHALG_002349"
     CDS             3677..4450
                     /locus_tag="ACHALG_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFN2587710.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAASPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            4464..5276
                     /locus_tag="ACHALG_002350"
     CDS             4464..5276
                     /locus_tag="ACHALG_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFN2587711.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            5330..6295
                     /locus_tag="ACHALG_002351"
     CDS             5330..6295
                     /locus_tag="ACHALG_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFN2587712.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            6432..9155
                     /locus_tag="ACHALG_002352"
     CDS             6432..9155
                     /locus_tag="ACHALG_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFN2587713.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESQILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGGSIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            9168..9800
                     /locus_tag="ACHALG_002353"
     CDS             9168..9800
                     /locus_tag="ACHALG_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFN2587714.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            10008..10232
                     /locus_tag="ACHALG_002354"
     CDS             10008..10232
                     /locus_tag="ACHALG_002354"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFN2587715.1"
                     /translation="MQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEVI
                     PENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            10334..10567
                     /gene="relB"
                     /locus_tag="ACHALG_002355"
     CDS             10334..10567
                     /gene="relB"
                     /locus_tag="ACHALG_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFN2587716.1"
                     /translation="MSQSTITFRIPDDEKELVLEYAKAHNSSLTELYRNAVLDKIEDE
                     IDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            10557..10826
                     /locus_tag="ACHALG_002356"
     CDS             10557..10826
                     /locus_tag="ACHALG_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFN2587717.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
     gene            complement(11005..12306)
                     /locus_tag="ACHALG_002357"
     CDS             complement(11005..12306)
                     /locus_tag="ACHALG_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFN2587718.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT         4031 a         1999 c         2422 g         3888 t
ORIGIN      
        1 ttccttagaa aaattaggtt gtcaaaattt caactcacgt tctgttaaag ggctagagag
       61 tagatttgaa agcatattac tggcttacag tgttctatta agtcgagcac aacgacgttt
      121 tgaaggaact ttgagatatt ctttaggata ttaaaattaa ctagcaccac gggtaaaata
      181 aatagttttt ctatcaagta tattgaacta cgataaattt gttattttgt tttgtttaat
      241 ttcgttgtac ttattggctt tgacttcgat ttctttaaat actgggtaat cggtgtccgg
      301 caaagctagc aaatctgttt tttctgtgtt ccatagatca ttgataatga catgtttttc
      361 gtaatggatt cgctctcgat cgtgtgacag ctccaccgct aatgcctcgt tgaaactggc
      421 gaaatccttc ataatcggtg tagatgaaaa gaagttgtac cgtacatagc cgactttgtt
      481 ttccatacta cctttctcgt ttccgctacg tggattgcac acctgtgtct caaaaccata
      541 gtgcatagaa aatcggagga aaccgtcagt cagcaccgct tgttcagtct ttgtttttgt
      601 ctaagtaacg gccgttgaca tattatcaat ccgtattttc cgaggaacgc cacctgattg
      661 gctgaagagt tctttcatgc cttccaacaa acattcttga ttctcggcgg ggagtacaac
      721 ggcgaatcct gcattactaa aagggaaagt catcactaat accttcacat ctttaaacgc
      781 tccatcgtga caaacttcca tcgtaccaaa atcaagttgc gcttctgctg gcgggtgttc
      841 aagacgctca aagccttggt cttccagtgt ttccaagtag tgtgtcgctt tccaatcttg
      901 aatgaataca cagactgttc ggtaggatcc tggaaagcca tcggctttta aagcagtagt
      961 cattgcttca gcagtcctac gctttttatt tggcagctta gcatcttctg aaagccatag
     1021 ggaaacgatg cctccccact tttctgtata catcatacca gtctttttgc gtgctttagg
     1081 ttgcggcagc aaatcatcat ctgcatattt tttagccgtg cgccaattta catctagctc
     1141 ctttgcaata tgattaatgg atttatcatg attatttcgt aaatttctga tagtattaat
     1201 atcggtcatt gtgagcatac ctttcttacc tccagttaca gtgtcgagac tataacttta
     1261 gaggagtttc aggttgcccg caatggcttt ttatcttaac ttaatcgatt tgcggagttg
     1321 tgtagttttt cgttgcactt gtatgtactt ttattctgcc ataaacagct taatgaatgt
     1381 ccacttgaaa aagtgaataa acgaaataga tgactcgatt taataaggaa gtaaacccac
     1441 acagaaatat aggtgatttt ccttgaatga ccaagatatt gaaagttagt gaaaaaacac
     1501 aaaaaggtag ccctattcac agaaggtctg ccttttgtgt tatcgccagc aaacattaga
     1561 gggggtattt catatagctt ttgtcctctc gacagtccta tcagtttctc ttttaactta
     1621 tctctaagtg ctgtccagtt ttaatagctt ctagcttgtt tcctaactgt gtttttaata
     1681 tgttgaaggc ttttgaacct gtgcagtggc aagtaaggat tttttctact cgtgagtcat
     1741 tcaatttatt tccaattttc attacctctt gctcaggtag acctaaatta tttatataag
     1801 gtatgccaat catatggaag ccaccaatta ggtattttat gggtttagtc ggaaatgttt
     1861 caagagcaat ttccatcata tcgataatgc catgatgact acagccagta aagacaatca
     1921 gaccgtcttc ttgctcaatt acgaccaata attcatctga aaaatcatca taaacaaatt
     1981 tatcatccat ctttttgtat aaattcttgg tggtcgagtt tgctttgcct agggggatga
     2041 tatggaagct cggagcaagt tctgttttcg tttttattgt atgtaaattc tcatgcattt
     2101 taaggatttc atttttagga ataccaacat acagaaacgt ggttaaaatc tttaaataat
     2161 ggggtaaagt tacaccttca gctgtataaa taggggcgtt tcctgatttt aaatatctta
     2221 acccgccggt gtggtcagaa tgaccgtgtg aaataatcac tgcctccaga tcattcaaag
     2281 tttcccccat tttagtcgca ttatagacta attttttatt agcgccagca tcgaagagat
     2341 atttcttccc tgagtagtta attaagagag ataacccatg tccactttta aagttcaaat
     2401 ttattcttga gttttcaaat aaaaccttga tttccactat aacatctcct ttcacatgaa
     2461 cgtgtattca tatattgtag cacattaagt tctatttttt gttgtaatta ccgaaaaaaa
     2521 tattaatgga cgctttttac gctttatttc gtatgtggaa gaaaatttct taggatgtgg
     2581 cgggggattg gggcaaagct aaattccaat tacagtgatc atttaattag acacctttaa
     2641 atacccacac tttcatcaac actataattt agggaaagaa aagtgttggc atgatataga
     2701 aaaaccttta attaacagcg tttcctaaaa ttggcacgca tcttgcttat agaaaagcgt
     2761 aaggatagga ggaatgaaaa tggcaactat aattcttgcc agccatgggg aatttgctca
     2821 aggattaaag caaacagcga caatgattat tggagatagt gtaccaattc atgccttgtc
     2881 agcatttaga gatgaagatg aatctatttt ggtacaaata aaaaaacttt tagccacaat
     2941 agatatagaa gaaacctaca ttttaactga tattctaggt ggtagtgtga ataatgatat
     3001 gctcactatt attaaagagt atgagggatt gcaattaatt actggaatga acttaccttt
     3061 agttatttct attgcaacct ataatggaaa aattggggaa agtgagctag aaaagcttat
     3121 taaagagagt caacaatcag tgattgattg tagaaaatta ttagtagaac aacttactgg
     3181 aggagatgac ctatgattaa gttagtacgt attgaccatc gtttgttgca cggacaggta
     3241 gtattttctt ggagtaaatc attaaatatc aatcgtatta ttattgccaa taaagaggca
     3301 gcgaatgatg aattcaaaaa aatgtctttg aatttatcta aaccgactgg cattaaatta
     3361 acaatttttt cagttgaaca gttactagaa aaaatgccta aaatcgaaac acttaacgaa
     3421 aatatcatgc ttatttttgg taatacaaaa gaaattcgtc aattttgtga agcctatcct
     3481 aaagtacaag aaatcaacta tggagggata atcaaaaaag aaggttccaa gcaatttagt
     3541 aatgccattt tcttgaatga atctgagcaa gaagatgcca aaaaattaaa agctcttggc
     3601 attaaacaat ttattcaaca ggtaccaact tctaaaaaag aagatttgaa tacaatgtta
     3661 taaaggagga atgaaaatgc tcgttcagtc aattggttta gggttaatcg gtgttttttg
     3721 tattttagac tcaaggcttt taggaagaat gaattttgaa cgaccgttga ttgtcagtac
     3781 gcttgttggt ttggtacttg gagacatgga aaaaggatta attgttggag cctctttgga
     3841 attgatgtcc ttaggattag taaatatcgg agctgcttca ccacctgata tgaatatggc
     3901 atcaattatt gctacagcct ttgccatttt atcaaatgcg aatgcagaag cagcattaac
     3961 gattgccatt ccaatttcag tgttaggtca aatactaggt atcttaatgc ggacgatttt
     4021 gtcaaatttg actcacgttg cggatcgctt tatcgaacaa ggaaaattta gccaagcaag
     4081 gagaattcat atactttatg gctcgggatt atatgctctc atgtatttta ttcctatttt
     4141 tctagcaatt tattttggaa cgaatcttgt ttctcagatt gttgatgcga ttcctgagtg
     4201 gctaacaaat ggcttaacgg ttgcaagtaa aattttacca gcatatgggt ttgcgttgtt
     4261 attacaaacg atgttaacga aaaaaatggt gccattttta ttacttggat ttttaattac
     4321 tgcttattct ggattgggaa tcattggtat tgcagccttc gcatgtattg ttgcgtttgt
     4381 catggttcaa gttcaaggta aaaactcaaa tggtaaccaa aatgagccag atccattaga
     4441 tgacttataa ggaggtagat taagtgaaag agaaagatca aaatgcatgg aaatatcggc
     4501 agttcttatg gcgttcatgg gcaattcaag cctcatggaa ctatgaacga caaatgaaca
     4561 tgggttttat gtacggaatt gcaccaattc tagataacat ttacaagaat ccagaagatg
     4621 aagcaatgaa gaaagaagct tacgagcggc atatgatgta ctacaactgt acccctcaaa
     4681 caagtgcatt tgtgttaggg ttaactgctt ctatggagga gcaatactac gaagaccaag
     4741 aaaactttaa tccggaatca atcagtgcag tgaagacttc tctgatggga ccgctatctg
     4801 gtgttggtga ttctttcttt caaggaacga ttcgggtatt agcctttgga ctaggaatta
     4861 atttagccca acaaggtagt attttaggtc caattctagc aatgttaatt tcctttgtac
     4921 cttccttttt agttacttac tatggtggga aaattggcta taacatgggg aataaatact
     4981 tgtctaaact gtatcaagaa ggtttgatgg ataaagtaat gtatgtttgc tccattgtgg
     5041 ggttgatggt cattggttcg atgattgcca gcatgatcgg gattacaaca ccaattcaaa
     5101 tgggtaaagc ttttgtctta caagatgtat tagatagtat tatgccacaa tttttaccat
     5161 taggattaac cttcttcatg tactggttat tgcagaaaaa ggtcaaaaca ggatggatgt
     5221 tggcaatctg tttagtcgga ggaattattt ttagtttgtt aggtatctta caataaggga
     5281 ataaccaata aatttggttg aataatataa ataaggagga attcaattga tgaattcaaa
     5341 agaaattgtt aaagaaatcg tagcgaaaaa agaggtaaaa tcaatcgtat ttgttggctg
     5401 tggcgcttca aaagcggacc tttatcctgc aaagtatttt ctagaccaaa atgcgaagca
     5461 gttacggatt agtcattaca cagccagcga atttaactat gcaacaccag catctgtaga
     5521 tgagtcaact gtcgttattt cagcttcttt aggaggtacg actccagaaa cagttgaagc
     5581 taacgcagta gctaaagaaa aaggtgctac agttattagt ctaacacgaa gcattgactc
     5641 accgttaaca aaagatgcag attatgtaat ttatcatcga tttgcagaga actatgctgc
     5701 taaattagaa aaaacaggct actgtttaga attggcagta gaacttttac atcaagtaga
     5761 aggttatgac cattatgaag caatgcaaga aggatttgct actatctatg atcttgccca
     5821 aaaaagtgca gaatcagctc gtaaagatgc gaagaaattt gctgaacgct ttaaagatga
     5881 aaaagttatt tatgtaatga gtagtggtgc tacacatgaa gtagcatact caacatcaat
     5941 ttgcttgatg atggaaatgc aatggattaa ttctggtacc ttccattcag gagaattttt
     6001 ccacggacca ttcgaaattg tggataaaga cgtaccgttt atattattaa tgaatgatgg
     6061 aaaaactaga aatatagatg ctcgagcatt aactttcctt gaacgctttg atacgaaaac
     6121 agaagttgta gatgccttgg attatggctt gtcttcacat attgctaaag aagtcttaga
     6181 ctactttaac ccattcgtga ttactgcagt atttagagtt tatgcagaag aattgtcaga
     6241 agcaagagaa catccattaa ctaagagacg ttatatgtgg aaactaactt attaaaaaat
     6301 agatcaaact aaataaggtg gcttgagaaa ttaagccacc ttgttttctt caatgtgatt
     6361 ttatacatat ggtaaaataa ctagtacata tggtaaaata actagtacat attgtgaaag
     6421 caggtggcaa gatgcagcga ttaaatgact atctttatca actaacattg cttttgaaat
     6481 ctacgccttc agataattat tcagctcgca gtatttcgca agctctacag ttgaatagaa
     6541 gtacaatcag tagctactta aatgaaggtg ttcgtgaagg gcttttcata aaagtaaaaa
     6601 gttacccggt tttattttta catagaacag ctcttgaaga actacatatc acactgaata
     6661 actccgaaat agaatcaatt gaaagcttgt taacaatttc tcaaaaaccc gcattagatc
     6721 aagtaattgg ctctaaagga agtttgaaag aagcaattga tcaaattaaa acggcagtct
     6781 tatatcctgg aaaagggttg cctttattat tgatcggcgc tagtggatca gggaaaacct
     6841 ttctagcgaa taaaatatat gaatatgcag tagaagaaaa ggtcattcac acatcggcac
     6901 ctttcataga ttataattgt gcacaatatg tgagcaatcc agagttacta tcctctgctt
     6961 tatttggcta cacgaaagga gcatttaccg gagcaagcca ggagcatact ggtttgctag
     7021 aaaaagccga tggaggagta ttgtttttag atgaagtgca tcgcttatct gaagaagggc
     7081 aagagaaatt atttacattt atggatacgg gtgaattctc tcctatgggg gataatagta
     7141 ttcggaaaaa agccgatgtt cgattagtct ttgcaacaac agaaaatata tatacgactt
     7201 tcttaccaac ttttcttagg agattgccag ttattgttaa tctacctaga tttcaacaac
     7261 gtccgtcgtt tgaacgtctg tcattgattg atgaattttt tgtttctgag agccaaattt
     7321 tagctaagga actatctgtt tctgatgcat tgattcattt cttaatgaat agtgattttg
     7381 aaggtaacgt ggggaatatt aaaaatatta tcaagtatgc ttgcggaaac gcatatatcc
     7441 accagaagag tcagcaaaca attcaagtta aattattgga tatgccttta gagtacagtt
     7501 ataaattcaa ggagcaacta aataaaccca gaaagaaaag agcagatcga atctactatc
     7561 cagaagaaac acgacaagtg caactagaaa gaaacaatca gctttttgtt caattttttg
     7621 accaattact tactggattc tctgaagtga ttgaaaataa caaggatatt aaactatttt
     7681 tagaagaaat ggttaccaag gtaacacaag taatggatac gcttattttt caagaggatt
     7741 acgaaaaaga gcagtcttta tacgccattc taaactatca tattcgtcaa agtattgatt
     7801 ttatgcgaga aagctacggg tttgaacaag atggtaaccg ggtagtcgct ttggccaact
     7861 atctatatgt caaggataat caagtgattt ttgaaaatag tcttcaagaa aagaaaacca
     7921 aagatcagct taacgaattt tttgctaatg ttcttgaaac cccttattgg tatgcaaaaa
     7981 aaatcttaag ccttttatct gagtgtttag atcaacctgt gtatgaagag gacattttct
     8041 ttattacgct atatttttat agtttgaatg tcacgaatgt taaaacaggt attaagagta
     8101 ttgtgctagc tcatggttat tctacagcta gtagtatggc gaatgttgcc aaccgtatgc
     8161 tgaataagaa cgtttttcaa gcttatgata tgcctttaga tattacatta gagaaaattg
     8221 aagcaaaatt gattcaattc cttggagatt atcgaacaga ttcaggcttg attttattgg
     8281 tagatatggg ttcactaaac caactaggcg gtttattagt gaatcatcta aatggaccac
     8341 tgttaatcat tgatcatatc aatacaccgt tattattgga agtcggccaa caagtattaa
     8401 acaataaaag tatcaccgaa ataggagcag aaattgaaac aagtcttact ttcaaaaagc
     8461 agttactact tcccaagaaa aagaaaaaga aagccatcat tacttgttgt tatacaggga
     8521 ttggtagtgc gacccagatt caggagattt taacgaaatg cttgaaagat gcagttagag
     8581 aattagtaat tattccgtat gattacaaaa agctgttaga aaacaagaat tttgagactc
     8641 cttttcaatt atacgatgtc atcatgattg taggtacaga agatcccaaa attaatcaga
     8701 ttccatatat tggattagat cgcctgatta atgggggaag cattggcgaa tttgttcatt
     8761 acttggaaaa agagctgact attgaagtga acgatttaca aaaagaatta attttcaatt
     8821 tttcaattag taaaattgtc gaaaatttaa ccattttgga tccagataag gtgctatctt
     8881 ttgtacaaaa agcaattaaa gaattatcga agttattaaa ttttgatttt tctaataatc
     8941 agctgttttt gttgtacctt cactgtagtt gtatgattga acgaattgta cgtaaagaaa
     9001 gtgtggatga gcaagaggac attgaagaat atatgcaagc aaatggagaa ataatggata
     9061 aaattcatag cgcttttttt gagattgaaa gggaatacac aattgaaata ccagatttag
     9121 agttgcgatt attgagcgat attgttcaag gataggagtg aattcgaatg acaatgggag
     9181 taatttttga tatggatggg gtactcatag atagcgagac attttatttt gagagaagaa
     9241 tgaacttctt tcaagaactg gagatagatc caggatcaaa ccagattgat gattatgtgg
     9301 ggaaaacaga agctgcaata tgggaaacat tagttcctga ggacaaatca ttaagagagg
     9361 aactttatcc gaaatatgta gattatcgaa aaacgcatcc aattgatttc gctaaagctt
     9421 tacgaaaaga agtgaaaccg ttacttgact tcctcaagaa gcgggatata ccattggcac
     9481 tggcatcttc ttctccagct gtcgagatag agagtatgct cattcaatgt gaacttactg
     9541 aatatttttc ctttgttata agtggtgagt cattaaaaca aagcaaacca catcccgaaa
     9601 tatatttaaa agcgaaacag caattagact gtgacttctt tattgcagta gaggattcaa
     9661 cgattggtat taaatcagca aaggcagcaa atatctatac ggcggcacta aaacaaccaa
     9721 tgcaaatcga tcagagtgaa gcagacatac aaattgaaag tctacaagaa ctggaacaaa
     9781 ttgttttaga aaaaggttag tgaattaatg gaaataaggc tatatatctg tgctagctat
     9841 tttgactaac caaaaaaact tgctataatt caggtgtagt aaaaggtgct ggtaacacct
     9901 tttacgaaaa tgcttcggca gtgataatgg gattggcttt ttaagttttt tattttgttc
     9961 gcaatcccta aggtcatgtg aatgatccta gggattgcta gaatattttg caacagaacc
    10021 aaaatacgaa tagttatcaa gcagccatat tgactgcggt caatttagga ggggatactg
    10081 atactactgc tgccgtaact ggtgctcttg cgggtattct ttacgggggt gaagtgatcc
    10141 ctgaaaattg gagaaaatcg cttcaaaatg accagcttct taaaacgatc agtcagagat
    10201 tttgcgatac cgtgctatct caaagaattt gaataatgga tatgatgcaa atcttgtttg
    10261 tactaacgtt gtattgacaa aaaggatttt atttagtaca atgttagtac aaatgatgaa
    10321 cggaggtaga atcatgtctc aatcaaccat tacatttagg attcccgacg atgaaaaaga
    10381 actggtatta gaatatgcaa aagcacacaa ttcttcattg accgaacttt atcgaaatgc
    10441 tgtactggat aaaatcgaag atgaaatcga tctacggact ttacggagtg caattgaagt
    10501 ttccaaagag aaaaaagaga gtggtatttc tcaagaggaa atggagaaac tgctaaatga
    10561 attataaagt catctacatg ccgcaagccc agaagcaatt aaaaaaaatg gacagaaacc
    10621 aagctaaaat aattgtcgcc tggatcagaa agaatttaga aaacacgact gacccgcgtc
    10681 agcacggaaa aggtttgact ggaaataaga gtggggaatg gcgctatcga attggaaact
    10741 atcgaatatt ggctaatatt tgtgattctg aaattgaaat ccagatattc tcaattggac
    10801 atagaagtac aatatataaa agataaccgt ttttggaacg aagtagagtt tcgaaagtca
    10861 atgtataatc ttttgaattt tgcataatac cacaggtaga aactacccga tactatcggt
    10921 tggtttctgc ttttttatgg ctctttgtca aataggactg atagagtgag ataagaacat
    10981 ctaaggtaga attccttttc tactttagat gttttttgat tcataggtaa tcagattatt
    11041 tagtaagaaa attctggtct tcattttccg aaagcttcga aagccataag cgactcgttt
    11101 caatgctttg atgtgggtat gcagattttc tattttggca tttgaataag gcaattcgag
    11161 ggctaaacga atgccggatt catagtttat aaaagctttt tttatagaat gcagttcttt
    11221 tggaacggaa ttgggcatcg ttcttacaag tttaaagaaa gtgtccgcct ctttgtcgtg
    11281 aaacgcttga agaatttctt gatagtaaaa ataggcgatt cgtaattcgt cagagatcga
    11341 aagcagacga tccaccactt cactttcggt gagatacgcc caaccaaaat tgcgccattt
    11401 ggtccatttg tcataaacaa gcttgtcctg tcgtttgatc agaagccgag acaacgcctt
    11461 taattgtcga taatatttgg cttgtgttgg atcgtttcga tcaaagcttt tcatgattcg
    11521 tacgcgaata tcctcaaatt tacgaatgag atgtttgaca atgtgaaaac gatccacaac
    11581 gagttttgcc tttggaaaac attcctttat cagagaggca taagaaacat tcatgtccgt
    11641 cacgatgacc tttaccttca atcgtgcgcg tcgttcaaat tgcataaaat aagaaacgag
    11701 agcctttttc ttccgtgaag gaagaatatc cagtaatttt ccagtatcgc catccataca
    11761 agaaaaggag tagggaccga cttgattttt cagtgaacga aattcatcca ttaataaaca
    11821 acgcggtaac caatttcttt ttacttttgt catggggatc agagaagcga gtgtccgaga
    11881 aacggtcgac caagaaacgc cgcaatgctt agcaattgtc gtcattgctt gtttttcagt
    11941 tagctcttct aaaattttat agcgcacttg tttagcgata aagcagtttc tatcgacaat
    12001 gtaggtattc gccgtaaaat aggtgtggca gccacgacag tggtagcgtt gtttcgctaa
    12061 agcaagataa gtcagttggt tcgcacattt attaagttga atgagcgatt ttttctttcc
    12121 gttcttaacg atcatgcctt catgattcat ctgtttacac aaaggacagc gacgaatttc
    12181 tttacttaac gtcgcagaaa caacaagact cttgatattc ttaatgctct tcgattccac
    12241 atttgtgatg aaaatgtttt tatctttgat tccaagtagt agtttgatat gatgaagatc
    12301 aggcatacag aacgctcctt tgattgtgtt gtggtgattc
//
LOCUS       DAZABD010000077        12204 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000077 DAZABD010000000
VERSION     DAZABD010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12204
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.078"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..430)
                     /locus_tag="ACHALG_002358"
     CDS             complement(<1..430)
                     /locus_tag="ACHALG_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFN2587719.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGS"
     gene            491..1744
                     /locus_tag="ACHALG_002359"
     CDS             491..1744
                     /locus_tag="ACHALG_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFN2587720.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLIGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            2066..2293
                     /locus_tag="ACHALG_002360"
     CDS             2066..2293
                     /locus_tag="ACHALG_002360"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFN2587721.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            2416..2742
                     /locus_tag="ACHALG_002361"
     CDS             2416..2742
                     /locus_tag="ACHALG_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFN2587722.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            2810..3523
                     /locus_tag="ACHALG_002362"
     CDS             2810..3523
                     /locus_tag="ACHALG_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFN2587723.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            3833..4684
                     /gene="licT"
                     /locus_tag="ACHALG_002363"
     CDS             3833..4684
                     /gene="licT"
                     /locus_tag="ACHALG_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFN2587724.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            4805..6670
                     /locus_tag="ACHALG_002364"
     CDS             4805..6670
                     /locus_tag="ACHALG_002364"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFN2587725.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            6684..8126
                     /locus_tag="ACHALG_002365"
     CDS             6684..8126
                     /locus_tag="ACHALG_002365"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFN2587726.1"
                     /translation="MQSRFSEKFLWGGATAANQLEGAYDRDGKGLSVADAMPGGKQRF
                     AIIGSEEFDWTIDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPN
                     GDEGTPNEAGLEFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYE
                     RFAQTVLERYSSKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVA
                     SSKAVKIGHELRSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGE
                     YPAYTARTHKKYGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSE
                     GNLLGGVKNPFLEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDG
                     TIIDDYRIDYLRRHIEAMANAVADGVDLMGYTPWGCMDLVSASTGEMSKRYGFIYVDL
                     DDEGNGTLNRSKKKSFDWYKEVIASNGVNL"
     gene            8234..8935
                     /locus_tag="ACHALG_002366"
     CDS             8234..8935
                     /locus_tag="ACHALG_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFN2587727.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQEILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            9074..9586
                     /locus_tag="ACHALG_002367"
                     /pseudo
     CDS             9074..9586
                     /locus_tag="ACHALG_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014566402.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            9597..10103
                     /locus_tag="ACHALG_002368"
     CDS             9597..10103
                     /locus_tag="ACHALG_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HFN2587728.1"
                     /translation="MDYKKRVQQMFLDSGVVLKETELERIEYTDFGLGAIETEGLNLF
                     VYENNERYCGKELVLLPYQTCPEHRHPPRDENEGKKETFRCRKGLVYLYVEGKETKKL
                     SCIIPKGQERYYTAKKEIILHAGDQYTIEQNIKHWFQAGATGAVITEFSSSSDDPSDS
                     FTNPAIYR"
     gene            10514..11089
                     /locus_tag="ACHALG_002369"
     CDS             10514..11089
                     /locus_tag="ACHALG_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFN2587729.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            11089..11529
                     /locus_tag="ACHALG_002370"
     CDS             11089..11529
                     /locus_tag="ACHALG_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFN2587730.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVLAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFEKLPVETYVGEYL"
     gene            11545..>12204
                     /locus_tag="ACHALG_002371"
     CDS             11545..>12204
                     /locus_tag="ACHALG_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFN2587731.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDTFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KY"
BASE COUNT         4055 a         1983 c         2609 g         3557 t
ORIGIN      
        1 gacttccacc tttttgatca ataaagattt tatcgacatt tacgatctgc tgttctttcc
       61 aattgatctg ttctagcact tgacgaattt gttcagataa attcctgtct tttctgatat
      121 atccaatcgg aaggattttc cgattgatga tctctactgc aaaattcgta taagcggtga
      181 aaaaaacaat attactatgt gcattttttt tacgtatttg ttcggcgaca tcaatacctg
      241 tgagtgattg cttcaagtcg atatcgataa agaaaacatc gtgattatca atagtgccca
      301 actggatcaa ctgattgatt tgagagatag aagtgagctg cacgatattc tcgatatgat
      361 gattgaccaa ttctctgatg atccattcgc tgtgaaactg gttatcttct atgataaaat
      421 actttttcat atgcaggctc ctattaaaaa aatatgtgta cattttgaaa caaaacaatg
      481 gaggaataag atggtcgcaa cagtgacact ttatttgacc tttactttga ttttagattt
      541 aggtatctat ttattctttt cgaaacaact gctagtaaaa aacagctttg gttgtatagc
      601 tttgatggtg gtgacacttt tacttcctat ccctaaagtg agctttcctt acctaattgg
      661 cttattattg atgtttcttt tttattttac tacgaaaaac ctgtttatca gctgtttgat
      721 cagtaatctt ttttttgcta tcactggact cagctttttt ctggtttttg atctgatgga
      781 ttatttagga aagagacatc ttggaacagc aagttttttt gcactactta tcgcatttat
      841 tttctatcta ttagtaaaaa gtatagacaa acagacgaga tttctcaact tgctttttac
      901 ttatccaact gaacactatt ttggaggtat ttgtatttta ttaagctggg gcgtgattta
      961 cttggtattg ggaagtgttc acttccgctc atctaattat ctgcttctat ctcttttgtc
     1021 gatagtcatc agtttttttt ctttgttttt ttccattatg ttgatcagtt tccaagtaaa
     1081 agaaagacaa catcaggaat cgcttcaact gtatcaaata aatgaagaat attaccatca
     1141 tctagaggaa ttcaaacatg attataaaag tctactgttt tcattaaaaa ttactcttca
     1201 ggaaaataaa aaagaaacgt tggaattttt agaaatatta gaagatcatt ctgcagctat
     1261 ttttgaaaaa ccgcaaatac aacagctaac aaatattgta tccccagctg tcaggggtgt
     1321 ctttttaaaa tttctagaaa aagcagaatc ggagaaaatc ccgatccaag tagttattcc
     1381 tgtcgaagtg aaaacaatcc cgattgatct ggttgttttt attcgctgtt tatctatttt
     1441 tatttctaac gcattcgatc atcgtgtagc cgatcagagt ccgattacga tcaatctgct
     1501 ggaagaaaaa gagggatcac gttttagtat agcgaacaag gcaaatccaa cgaatttgac
     1561 gctatcggag atggtcaaaa gaggaaaaac ctctaaaaaa aatggcgggt taggtctata
     1621 cagtgcgaaa aaaatgttgg atgtttacga aaatgcagaa ctaaaaatcg aattttcaaa
     1681 acagaatcac tatttttttg tagaaatgta tctggcatct agaaagagca aatcagccta
     1741 ctgacttact gtcaaaggat aagtttgagg aaagtctaca cggagataga gacaacaaca
     1801 cagcatattc aaaagaaaca gaagcgtgat gaccgttgtc acccttctgt ttcttttgct
     1861 atgtagttaa acaaagtagt tgacacttcc gctacttagt attagtacga aagtatactt
     1921 catgatacta agtagataag aagtgaaaag cggaacccac gatcgtcaaa atgctaactg
     1981 ctttgcttac accagataaa gggaagagca atatcgctta ttttaggcgt ggagaagcaa
     2041 gcaaatccct acgatcacaa aaaaaatgct gacccagcag ttaagagagt tagaacaaga
     2101 tgaaagtatc catcgaaaag cctataacca agtcccgcct aaagtagagt actcgctcac
     2161 cgaagaagga aaatcattaa gagagattct agtagcgttg tctgtttggg aagaagaacg
     2221 tatcgaaaaa caaaaagcct caagggaaga agacacgcta ctgcatacac atggtggcta
     2281 cctaaattat tgattttcta tgacatttat tttaaataaa agaaaacagg agttttttta
     2341 aaagcatcat ctatttttta tgtaaaaaaa tggtatgatt aatcaagtaa gaagtgattg
     2401 gaagaaggca agcaaatgaa tagtatggaa aaagtcaacc agtttagaga tgaacgtaat
     2461 tggcgacaat ttcataacga aaaagatttg gctatctcta tttctttgga agcatctgaa
     2521 ttgttagaac tttttcaatg gaaaacgccg gaagaagtaa aagaaacaca attagaacga
     2581 atcaaagaag aactggcaga tgtattgatc tattcataca tgatggcaga caaccttcac
     2641 ctggatctag atgagatcat tgaagaaaaa ttgatcaaaa ataatttgaa atatcctgta
     2701 gaaaaaagcg caggtgtacg aaaaaaatac acagagttgt agaaatgtgt cattcggctg
     2761 ttggaaagag caaaagaaaa acagtaaaat gagtctggag ggagagaaaa tgaaaaaaaa
     2821 aggaaaagca aaaagatggc tagtcaatat cctcttgttc ctcttgttat tagtaggact
     2881 agctttgatt ttcaatgaac agatcaagga ttattttgtc agagagacag gagataaata
     2941 tgctatagcg aatgtaacaa aagaagatct gaaaaagaat aacgataaag atgtcagttt
     3001 tgactttgat gcggtagaac caatgacgac agaaggggtc atgaggtcgc aaatgagagg
     3061 aacagatcta ccagtgattg ctagtatcgc agtcccttca gtttcaatca atttaccggt
     3121 tttcaaaggc ttggataata cgtcactact ttacggagca ggaacattat ctccagatca
     3181 agaaatggga aaaggaaatt atgccttagc aagccatcgt gcaacgaacc ctgaattgct
     3241 gtttacaccg ctagaaaatt tagagatggg tgccaagatc tatttgacag atttagaaaa
     3301 tgtgtataca tacaaaacat tttttaaaga aaaagttgct cctacagata cccaattact
     3361 gaatgaagtc gagggaaaag aaattgtcac actaattact tgtggcgaca tggacgccgt
     3421 cacgcgacta gttgtacaag gagaactgga aagtgttaca tcgataaaag atgcgacaga
     3481 tgatatgaga tctgctttca atctagagac aaaaacattt taggatactg ggacataagt
     3541 cgttacagat taaaaacagc caaaggatat gaatcgataa ggaaaaacca atttgctgaa
     3601 aaaaagagaa acgcaaagag gtgcatctct gatttggttg ttttctagat agacttgtgt
     3661 tccagttttt ttatttttta tatgaaaagg cttgcataaa taaaaattcg atggtatgat
     3721 gattttgaat aagaaatgga ttgttactgt acggcaggca aaaccagaat tcagctaaga
     3781 aacagtaggc gaattctagg tttttttatt tttcttgaga gggcgaagca aaatgaaaat
     3841 tgataaaatt ttgaataaca atgtcgtcat ttcgaaaaat ggattcgggg aagaagtcgt
     3901 ttgtatggga aaaggactag cttttcaaaa aaagattggg gacgagattt cgccagaagc
     3961 ggtccaaaaa gaatttgttt taagagattc ttttgctaaa aaccaattcc agcagctgat
     4021 ggctgatatt ccggcagagg agatcgaatg ggtcaaacaa gtagtcgaac tggctgaaga
     4081 taaactaaaa gttgaatttt cgcctaatat ttatctgaca ttgaccgatc atcttcatta
     4141 tgcgatcaca cgagcaaaag aaaatatcca actgcctaat ccattattat ttgaaacaaa
     4201 aaaattttat ccgaaagaat atcaagcagc gaaagaggct cttttgttcg tgaaacaaaa
     4261 gacgggtgtg gaactttctg aggatgaagc cggttttatt gcctttcatt ttgtgaatag
     4321 ccaacaagga aacgaagaca tgcaagtgac gatgaccgct accacaatgg taagagatat
     4381 actgagtatc atcagtaaat tcttcggcat cgtgttcgat gaagaatctt tgaattatca
     4441 gcgtatcatt actcatctgc agttttttgc ccagcgttat ctcaaagggg aaggatctga
     4501 cgagcaagat gaatttctct acgcattagt tcaaggaaag tatccaaaag cgtttcattg
     4561 tgtcgaacgg atcaatgagt atcttcgaca aacgcaaaaa caggaaatgg ggatcgcaga
     4621 gcagatttat ttaacgattc atattcaaag agtagtaagt gagaaaaaaa gcatccaatc
     4681 ataaggattg ttactgtatg gcaggcaaaa cctggattga aaaaataggt tcacgaatgt
     4741 ttgtgactgt ttttttcaat ctgggttttt attttttaaa taaaaaagaa agaaggaaaa
     4801 aacaatggat aatcaagcag ttggtcgtcg tgtatgggaa gcggtaggag gacaaaagaa
     4861 tgtcaaaagt ttagtacatt gtgctacgcg gttgcgattc agattaaaag atgaatcttt
     4921 agcagatacg caaaagttaa aagaagaccc ggacgtgatc caagtcgtac aaagtggcgg
     4981 tcagtatcaa gtagtgattg gcagtaatgt agcagatgtt tatcaatcga tcgtggatga
     5041 ggaaggtctt gcagtttcgg aggatggaaa agaagagtcc aaaaatattt tgaatcgtct
     5101 gatcgatatt atctctagta tatttacacc ttttctaggg gcgatggcag ccgccggggt
     5161 attgaaagga ttcttatcat tggcaacggt gatgggctgg ctgacagctg atagcggtac
     5221 ttatcaaata ttgtttgctg cagcagatgg tgtctttacc ttcttacctg tcatgcttgc
     5281 ttttacagca gctaaaaagt tcaaagccaa ccagttttta gcagtagcaa ttgctatggc
     5341 acttgtgtat ccagctatca cagcagttgc tggtgcggga gaagctatca gtttctttgg
     5401 gatcccagtg attctttcac catctggtta tacttcttct gtcataccaa tcattctagc
     5461 cgtatgggtt caaagtaaat tagagccatt cgtaaaaaaa gtgattccgc aatttctgca
     5521 aatgatttta gttccgcttg tcgttttagt agtgatggtt ccattaacat ttcttgcttt
     5581 aggaccaatc gggacggttg ccggtaatgc tttaggcggt ctcttcaact cgatttatgg
     5641 atttagtcca atcgtagcag gtctaatcat gggaagttta tggcaagtat tcgtgatgtt
     5701 cggcatgcat tggggattcg taccgatcat gtttttgaat atcgaacaat atgggtttga
     5761 tgtattgatg ccaatgcttc ttccagccat cttggcacaa ggtggggctg ctttagctgt
     5821 agcattgcga acaaaagata cgaaacttcg agcattaggg atttcttcta cagtgacttc
     5881 gttatttgga atcactgaac caactgtgta tggtgtgaca ttacctttga agaaaccatt
     5941 cattgccgct tgtatttctg gaggtattgg tggagctatt atcggatttt ccggagtaaa
     6001 agctttctcc agcagtttgg tcagcttatt gacgattccg actttcatta atacggtgga
     6061 tggtgtagag tcaaatgtga cagtagcagt aatagctaca ggaattgctt ttgttcttgc
     6121 attcgtaggt acattgattt taggatttga cgaacaaaca caagacaatc aattagaaaa
     6181 caaacatgcg aatgcaggag aaccgattac ttcagcacgg cataccttaa aaagtccttt
     6241 aacgggtaaa gtactgccat tgagtgaagt accagaccaa gttttttctt ctggcgtgat
     6301 gggaaaaggt atcgctattg atccagaagt aggagaacta gttgcacctg cagacggaga
     6361 gatcacgact atttttccaa ctggacatgc tgtagggatc acaacgactg acggagcgga
     6421 gattttgatt catatcggga tggatacagt agaattaaat gggaatggct ttgagatctt
     6481 agtcaaacaa ggggatctcg tcaaagcagg agatttattg attcgttttg acatcgaagc
     6541 aattagagca gctggctata gcgtaatcac tccagttgtc attacgaata ctgatgcatt
     6601 tgccgatata cttgaattag accaaaaaga gattattgca aacgaagatg ttctggcaat
     6661 tgtaaaataa ggaggaaatc aggatgcaat cacgtttttc agaaaaattt ttatggggtg
     6721 gagcaactgc tgccaatcaa ttagaagggg cttatgatcg agacggaaaa gggctatccg
     6781 ttgcagatgc tatgcctgga ggtaagcagc gctttgcgat tatcggcagt gaagaatttg
     6841 actggacgat tgatcaagaa aaatatattt acccgaatca tcgaggaatt gatcattacg
     6901 atcgtttcaa agaagatctt gccttgtttg cggaaatggg attcaaatgt tatcgttttt
     6961 cgattgcctg gacacgaatt tttccaaatg gagacgaagg tactcctaac gaagcgggat
     7021 tagaatttta tgatcagctg attgatgagt gcttaaaata tgatattgaa cctgtgatca
     7081 cgatttctca ttacgaaatg cctcttcatt tggcaaaaga atacggtggc tggaaaaatc
     7141 gaaaactgat cgacttctat gaacgatttg ctcagacagt attagagaga tacagcagta
     7201 aagtcaaata ttggatgact ttcaatgaaa tcaattcggc ctttcacttt ccagctttaa
     7261 gtcaaggatt agtaaaaagc aatggagcag gagagtacca aaatattttc caagcatggc
     7321 ataatcaatt cgttgcaagt agcaaagccg taaaaatcgg ccacgaattg cgttcggata
     7381 tccaaatcgg ctgcatgatt atctatgcaa caacttacag catcgatgcc aatccagtca
     7441 atcaggcagc aacaatgatc caaaaccaag aattcaattt tttctgcacg gatgtccaag
     7501 tacgtggaga ataccctgca tacacagcgc gtacccataa aaaatatgga gtcgatccgg
     7561 aaaaattaga gcaaacggaa aaagacttcc agttgctaaa agaatatcca gtggactaca
     7621 tcggatttag ttattatatg tctacggcaa tcaatgagac tgatccacaa gcagcaactt
     7681 cagaagggaa cttgctggga ggagtaaaaa atccgtttct tgaggcaagt gaatggggct
     7741 ggcagatcga tccagaaggg ctgagaatcg cattgaatga attatacaat cgttaccaga
     7801 aaccattatt cattgttgaa aacggtcttg gagccattga tcatatggaa gaggatggca
     7861 cgattattga tgattatcgg atcgattatt tacgtcgtca tatcgaagcg atggctaatg
     7921 cagtagcaga cggtgtagat ttgatgggct atacgccttg gggatgtatg gatcttgtca
     7981 gtgcatcaac aggagaaatg agtaaacgtt acggctttat ctatgtggat ctagacgatg
     8041 aaggaaatgg gacactgaat cgatcaaaga aaaaatcttt tgattggtat aaagaagtaa
     8101 ttgcctcaaa tggcgtaaac ttgtaattaa agcagctgaa tgaaaaagaa cacacttctc
     8161 acatgtgatt tgtgttcttt ttttattttt tatgtgctat tcttttagta atagtaaagc
     8221 aaagggtgac gttatgaaaa ggacacgtgt actatacttg gaagtcgcag ataaaattaa
     8281 agaagatatt ttctcaggaa aatatccagt cggttcgatg ttaccgacag aaagtgaatt
     8341 agaagaactg tttcaagtca gcaaaatcac tgtccgaaag gcaatcgaac tgctggcatc
     8401 agatgaatat gtcgcaaaga aaagtggtcg tgggacgacg gttctaagca atcgtcctta
     8461 caatcgatta tccaaagcgg caagctttac gcaaatcttg gagaactccc gatatgaagt
     8521 ccggaaagaa acgctggatt tgcaattggt ccatataaag aaggaagatc ctttgttttc
     8581 ctttttcggt gagcaagctg tttgttttaa gcgcttatat tatttgaaca atcagcctta
     8641 tatttatttt gttcattatc ttccacaaga aatgatgcaa ttcagcagag aagaatttgg
     8701 caaagaatct ttgtatcgtt tgctgacgaa aaaaggatat actattgact cattttctga
     8761 tgatttctcg gcagtatttt taacagaaga agaacaagaa attctgaaaa cagacgcaca
     8821 gatcggcatc aaacggatac ggaaatcgtc tacgcaaatt ggggaaatga ttgaatattc
     8881 ggaggcggtc taccatacgg agctgcatcc ttaccatatt gattatgaaa cataaataaa
     8941 aaaagaggtt gtgaaaaagc tgccctgcat caagtaataa gaacagccaa acaaaaatag
     9001 cttctcatat tagcttctca tatttttcgt ttggttaggc ttattaccgc agatacccgg
     9061 ctgtttctgg attaccattt attctggata agggggtgtg gcacgatttt cagaaggaat
     9121 ctatgaacca atcactgtat tgattttagc ttcgatggta accccattcg gcttgacgat
     9181 cgcttatttc ttagggaaaa tcattcgtaa aaatatattc aatcgtcaag aaatcgatac
     9241 attgaagacc gcttttccta tggggatctg tcagatcaca gagggatgtt tccctatcgt
     9301 attgaacgac ttggcacgca atgtcatagc aacgggagtc ggtggggcag tcggcggtgg
     9361 attgagcatg ttttggggcg cagatagccg tattccggca tcgggaatgt ttgcagtcgc
     9421 tacgatgacc agaccatggg cattcattgg tgcattgtta gctggttctt ttgctacggg
     9481 aataactatt ttattattga aaaaaccagt tgacccaaat gcagaagtca tccaagaaga
     9541 aaaagaggaa gaagagatat cttgggatga cttgacaatc agttaggagg aacagaatgg
     9601 attacaagaa aagagtacaa caaatgttcc tagattcagg agtagtattg aaagaaacag
     9661 aattagaaag aatcgaatat acagattttg gattaggagc aatcgagact gaaggattga
     9721 atttatttgt ttatgaaaat aatgagcgat actgtgggaa agaactggta ttattgcctt
     9781 atcaaacttg tccagagcat cggcacccac ctcgagatga gaatgaagga aaaaaggaaa
     9841 cctttcgttg tcgaaaaggc ttagtatatc tttatgtcga aggaaaagaa acaaaaaaac
     9901 tttcctgtat tattccaaag gggcaagaac gttactatac ggcaaaaaaa gaaatcatac
     9961 ttcacgctgg agatcagtac acgatcgaac aaaatattaa acactggttc caggcaggtg
    10021 caacaggcgc ggttatcacc gaattttctt ccagcagtga tgatccaagt gatagtttta
    10081 cgaatccagc tatttatcgt taatacaaag gtttgggaac aagctgatga accaaaatga
    10141 tcaaatttcg attatttggt tcgtcggctt ttttatttag agaaacatca ggcgttggtg
    10201 aaacataaca cgagaataaa atggattttt cattaatagg agaaacaagt gtgtgactat
    10261 ttaaataatg cgtgttattg acatgcagga cgaaatccca atttcttcta taagtaagtc
    10321 gtttcaattt tatatttact agttatttat ttttttggtt actgtaaaat agctgaaaaa
    10381 ttattttttt gcaaatacta tatatagtgg tgtttgatct atataccact aaaaaactac
    10441 aaaatctagt cttttatttt aaaaaaacac gtgtagaatg aagatagact tttgagttag
    10501 ggagtgtttt tttatgaaac ttactgtttt ttgcggttcc cgttttggca ataaagaaag
    10561 ttacaaattt attgcgcaaa cgttaggaaa atatatggca caagaagaaa tcgaactggt
    10621 ttacggtggt tcatactccg ggattatggg agtgattagt aaaacagtac tggaaaataa
    10681 cggaaaagtc acaggtatct atccaaatgg cctttttgaa actgagctgc ctgggaaaga
    10741 tgttactaca tttattccta cagagacaat tgatgaaaga aaggtattat tatttgaaaa
    10801 aggagatgct gtcctcgttt tccctggagg tctaggtaca ttggaggaat tttcgcaact
    10861 tctttcttgg atagcgatcg gcctgacacc tgataagcca atcggaatat tgaatattgg
    10921 cggttattac agcggcttgc aacaattatt agaaactttt gcaaaagagg attttatgga
    10981 taaaaaatgg ttggatcaag tgatcttttc caacaatcca ttggaactgg tcaatgaatt
    11041 acgagcagta tctattaaaa atcagttatt attgaaggag gcaaaataat gagtcaaata
    11101 tatttagcag gaccattttt ttcagaggag caaatcgatc gagtaagtcg tatcgaaaaa
    11161 gcgttggagg aaaacaaaac agtcacaagt ttctactctc cacgtcatca ccaagaaagc
    11221 aattatgaac tattcagcgc tggatgggcg caggaagtat acgaaaaaga tatggaagag
    11281 ttgacaaatg cagaatttgt tttggctatc cttgactttg agcatcaaac aatcgatcca
    11341 ggaacggctt acgaattagg tgtggcaact atgttgaaaa aaccgatgat catcgtacaa
    11401 gaagagacgg tacctacgaa cttgatgatt acacaaagtc ttcacacgta tttaaaatca
    11461 gaccaagctg tacgtgaata tgattttgaa aaattgcctg ttgaaactta cgtaggcgag
    11521 tacttataga agaggaaggg ttgtatgtca gtgattttga tttcaggaac gattggtgca
    11581 ggtaagagca gtttgacgga tatgcttgca aaagagattg attctaaacc attttatgaa
    11641 aatgtcgaag ataacgaagt attgccgctt ttttattcaa atcctgagca gtacgcattt
    11701 ttattgcaaa tcttcttttt gaataagcga tttttagcaa tgaaaaatgc tttagtgaac
    11761 gatgacaatg tgctagaccg ctccatctac gaagatagtc tgttgttcca tctaaatgct
    11821 gatttaggtc gggtaacgga tatcgaagta cagcagtacg atagcttatt agatacgatg
    11881 ctaaacgagt tagatgatgt tgctccaaaa aaacgtcctg atttgatggt ccatatcaaa
    11941 gtttctttgg acacgatgct agaacgtatc aaaaaacgcg ggcgtgacta tgaacagcta
    12001 gaaagcgatg aaacgttata cacctattac gaaacactga acacccgcta taatcaatgg
    12061 tatgaggatt ttgatgtatg tcctaaaata caagtggatg gggatacatt cgattttgta
    12121 gaaaatccag aagatgcaaa aaaggtgatt cgtcagatcc aagaaaaatt agctgaattg
    12181 gaaggaaagc ctgctggaaa atat
//
LOCUS       DAZABD010000078        12104 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000078 DAZABD010000000
VERSION     DAZABD010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12104
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.079"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            39..542
                     /locus_tag="ACHALG_002372"
     CDS             39..542
                     /locus_tag="ACHALG_002372"
                     /inference="COORDINATES: protein motif:HMM:NF024954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFN2587732.1"
                     /translation="METERQIRELEKAMNTTDNRRLYERFLAVKLVLEGHTRKEAGHT
                     IGRDEHTVSHYVKNYCHNGIQGLVSKKQSGRPSRLTDEQEAELVQIVAYHTPEEVGFK
                     SRANWTLAIVCDLILREWSYGYTQKGVADLHNFPPRYYEYPGGVIACVTAYANLQHRS
                     KWLSSKK"
     gene            complement(755..1510)
                     /locus_tag="ACHALG_002373"
     CDS             complement(755..1510)
                     /locus_tag="ACHALG_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFN2587733.1"
                     /translation="MDFEQNVRLNFDLLSDNEKDMVFFIRTHKAQVVHMSIVELGEQL
                     LSSKSSVLRLAKKLGFRGYSEMKYSLEQSIVQSVIPPTDLIALLKDEINRTFQLADQT
                     NFQPILEQLKAAKTVVVYATGFTQNNFSKDFVNDLILSGRPAMLISGETNFEMLSHTL
                     TKDDFVIVTSLSGETPSIKSTIKNLNMNRIPLCGVTELGKNFLSEHSDFLLYYETREL
                     PSNLIEGSRSMIGLNILLSILSRKYREFILFDE"
     gene            complement(1617..3287)
                     /locus_tag="ACHALG_002374"
     CDS             complement(1617..3287)
                     /locus_tag="ACHALG_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFN2587734.1"
                     /translation="MNEWWKSSVVYQVYPQSFKDSNNDGIGDLMGIVEKLPYLAKLGV
                     DVIWLTPIFDSPLIDNGYDIRDYEKISENYGTLEDFKRLLEQAHQLNIRVILDLVVNH
                     TSNQHAWFIESQKNKENPYSDYYIWKDPKEDGSAPTNWGSTFGGSAWEYVESRKQYYL
                     HCFAKEQPDLNWENPKVRQSIYKMMKKWFDLGIDGFRMDVISLISKHPDYPDAPNSMP
                     YTKSYYVGASNGPRVHEFLQEMNREVLSKYDSLTVGETPNTNSRQAQLYTFPERKELN
                     MVFHFDHMHLDYGKYGKFSDVRFKLSDLRATLSEWQEALSDGWNSLYWSNHDQPRAVT
                     RFGDENEYRIESSKMLGTLLHMMKGTPFIFQGEEIGMKNVPFSSIDEYQDIETNYFYK
                     KMKEEGESDEYIKRAIYLKSRDNARTPMPWDHSKGYGFSNSDPWISFSPDNQQINVED
                     ALKNPNSVFYHYQKLISLRKNYPIIQHGTYKLINENDSAVFGYIREYKNELLVVICSF
                     SKESVPYELPKSLQGRKGVLLLSNYEQSPSELEDKFILRPYEALVYHI"
     gene            complement(3291..3710)
                     /locus_tag="ACHALG_002375"
     CDS             complement(3291..3710)
                     /locus_tag="ACHALG_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFN2587735.1"
                     /translation="MKKIIFASHHNLAVGLKDTVQYILPNVGEITAIAGYIDNSPIEE
                     KINEVMVRDNEDTDILIFTDMLGGSVNQAFIKYINQPNVYLITGMNLPVVLTILLNTL
                     NKRLTDNHIREAIQEAQDQLVYVNDYLKNIVVDEEDE"
     gene            complement(3723..4532)
                     /locus_tag="ACHALG_002376"
     CDS             complement(3723..4532)
                     /locus_tag="ACHALG_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFN2587736.1"
                     /translation="MNNEGNKLSKKDIRRAAFRYMFMACNIFNYENQQGPAVVYGLEK
                     ALRKIYPNDEDYRASLNNHFKYFNTTTWMANLLLGASLAMEEKDGVSSLDAVQSFKTG
                     MMGPLAGVGDTLVWVLYPTIMGSIAAYMGLEGNPTGAIIWLCLNLFFLFFRFKLFSIG
                     YTSGLKLVTALGSKLSIFTESASIMGLTVVGALIPSVVKMNVAVVFQSGKVKLPIQTE
                     ILDKIMPALLPAVLTIIVYKLIASKKMTVIQIIFSVIIFALILSFFGILKV"
     gene            complement(4525..5325)
                     /locus_tag="ACHALG_002377"
     CDS             complement(4525..5325)
                     /locus_tag="ACHALG_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFN2587737.1"
                     /translation="MEIIQGILIIILAVWVVLDQQGLVITTWFPIMIALFAGLIMGDM
                     TTAMVIGGTFQLMALGVANIGGSSVPNWGLAALVGIYVAIRTTNNIEEAKAVALAVGV
                     PVGMLGIQLDVLAKILNTYVTHAAQKAANKMDFKKMNQILWLGPVIFGLSTAIPTALC
                     VVFGDRIVRLILDFVPKWFTDGLSIAGSMLPVVGIALLLQVMPVKKYMTMLLIGFVLS
                     AYLNMPILGISIVGFALAYYFFTTEIKKAETASPVAANIVDEGDDFDE"
     gene            complement(5340..5816)
                     /locus_tag="ACHALG_002378"
     CDS             complement(5340..5816)
                     /locus_tag="ACHALG_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFN2587738.1"
                     /translation="MGLVLARIDQRLIHGIVVTQWAGATKAKRLMVVDDQVSKDDTQK
                     AAMRMSKPAGTGMSIIDTDTAIINFKLGKYDSHNVLMIVKEPSTLVKLAENGIDIPKV
                     NIGIMFDGEGKQTVKKMVSVDEQEIADLKKLEAMGIPVTFHFVPSDPEEPLDVRIK"
     gene            complement(6211..6690)
                     /gene="rlmH"
                     /locus_tag="ACHALG_002379"
     CDS             complement(6211..6690)
                     /gene="rlmH"
                     /locus_tag="ACHALG_002379"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFN2587739.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAHISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            7119..8405
                     /locus_tag="ACHALG_002380"
     CDS             7119..8405
                     /locus_tag="ACHALG_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HFN2587740.1"
                     /translation="MDRKNVTPKMKKNKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTTSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSGF
                     GGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKDG
                     TKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYANS
                     VTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSKI
                     ASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQE
                     QILKIPSSVTNGVIVTNVQTATPAEKAGLKKYDVITKIDDKEVSSGVELQSALYKKKV
                     GDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(8580..9185)
                     /gene="ytpR"
                     /locus_tag="ACHALG_002381"
     CDS             complement(8580..9185)
                     /gene="ytpR"
                     /locus_tag="ACHALG_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HFN2587741.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(9390..9854)
                     /locus_tag="ACHALG_002382"
     CDS             complement(9390..9854)
                     /locus_tag="ACHALG_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFN2587742.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(9876..10196)
                     /locus_tag="ACHALG_002383"
     CDS             complement(9876..10196)
                     /locus_tag="ACHALG_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFN2587743.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            complement(10339..11418)
                     /gene="pepA"
                     /locus_tag="ACHALG_002384"
     CDS             complement(10339..11418)
                     /gene="pepA"
                     /locus_tag="ACHALG_002384"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HFN2587744.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            11618..11968
                     /locus_tag="ACHALG_002385"
     CDS             11618..11968
                     /locus_tag="ACHALG_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFN2587745.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
BASE COUNT         3646 a         2482 c         2076 g         3900 t
ORIGIN      
        1 aggcgtatac taaagaaaac acttgaggag tggggaacat ggaaacagaa cgccaaatcc
       61 gtgaattgga aaaagcgatg aatacgacag acaaccgccg gttgtacgag cgtttcctgg
      121 cagtcaagtt ggttcttgaa ggccacaccc ggaaagaagc cgggcacacc atcggccgcg
      181 acgagcatac tgtcagccac tatgtgaaaa actactgcca taatggaatc caaggtttag
      241 tctccaaaaa acaaagcgga cgaccttcac ggctgacaga tgaacaggaa gcggaactgg
      301 tccaaatcgt cgcctaccac actccggagg aagtaggttt caaatcccgt gccaattgga
      361 cattggcgat tgtgtgtgac ctgattctcc gggaatggag ttatggatat acgcaaaaag
      421 gcgtggcgga cctacataac tttcccccta gatattacga atatccaggg ggtgttattg
      481 catgcgtaac agcctatgct aatttacaac atagatcgaa gtggcttagc tctaaaaaat
      541 aagccgcatc tttgtcagat acgactgttt agcattgctc attcaattag ttgatgattt
      601 tttcatcaaa tttcagatat agttgaatat ttcctttgtt cagcttatca aatgtcactt
      661 tatcaatgaa tgttcgatag atttccttgt tctcactctt gaaacgattg gctaacaact
      721 tttcaacact tgctcctttg tttcgaaatg gaggctactc atcaaaaaga ataaattcac
      781 ggtactttct agagagtatt gatagcaaaa tatttaatcc aatcattgaa cgacttcctt
      841 caattaaatt gcttggaagc tctcttgtct cataatataa caaaaagtcg gaatgctcgc
      901 tgaggaaatt ttttcccagc tcagtaactc cgcaaagtgg aattcggttc atattcaaat
      961 ttttgattgt tgatttgatg cttggtgtct caccgcttag agacgtaacg atcacaaaat
     1021 catcttttgt caaagtatgg cttaacattt caaagtttgt ttcacctgaa attaacatgg
     1081 ctggtcgtcc cgataaaatc aaatcgttta caaagtcttt tgaaaagtta ttttgcgtaa
     1141 agcctgtcgc atagacaact actgtttttg ccgcttttag ctgttctaat atcggttgga
     1201 aatttgtttg gtccgctaat tgaaaagttc ggttgatttc atctttcaat agtgcgataa
     1261 gatctgtcgg tggaataacc gattgaacga tgctctgttc aagagaatat ttcatttcag
     1321 aatacccacg aaatcccaat ttttttgcta aacgtaagac agaactttta gaagacaata
     1381 attgttctcc caattctaca atggacatgt gaactacttg agctttatgc gtacgaataa
     1441 aaaaaaccat atctttttca ttatctgaga gcaaatcaaa gtttagtcga acattttgtt
     1501 caaaatccat tttctcacct ctcatatata cagaaggaat ttcggataaa acttgcgctg
     1561 ttttatccga aattctttct tattgcccaa attctaacac agtttttacg tgcttgctat
     1621 atatgataaa caagtgcttc gtaaggtcgt aatataaact tatcttccaa ttcagatggt
     1681 gattgctcat aatttgatag aagaagaacc ccttttcgac cttgcaatga tttaggtaat
     1741 tcatagggaa cagactcttt tgaaaaagaa cagatcacca ctaatagttc atttttatac
     1801 tcacgtatat aaccaaacac tgctgaatca ttctcattga tcaatttata cgtaccatgc
     1861 tgaatgattg gatagttttt acgcagtgaa atcagttttt gataatgata aaaaactgaa
     1921 tttggatttt ttaaagcatc ttctacattg atttgttgat tatctggcga aaaagagatc
     1981 caaggatcac tgttcgagaa tccataccct tttgaatgat cccaaggcat tggtgttcgt
     2041 gcgttatcac gagatttcaa ataaatagca cgtttgatgt actcatcact ctcgccttct
     2101 tctttcatct ttttataaaa atagtttgtt tcaatgtctt gatactcatc aattgaagaa
     2161 aatggaacat ttttcattcc aatttcttcc ccttgaaaaa taaatggggt tcctttcatc
     2221 atgtgaagta gtgttcctaa catcttacta gactctatcc gatattcgtt ttcatcacca
     2281 aatctagtaa cagctcgcgg ttgatcatgg ttactccagt aaagagaatt ccaaccatct
     2341 gaaagcgctt cttgccactc acttaacgta gctcttaaat cgctaagctt aaaccttaca
     2401 tctgaaaact ttccatactt tccataatca agatgcatat gatcaaaatg aaaaaccata
     2461 tttaattctt tacgctcagg aaacgtataa agctgtgcct gtctgctatt tgtgttcggt
     2521 gtttcaccga cagttaagct gtcatattta ctcaaaactt ctcgattcat ctcttgtaaa
     2581 aattcatgca ctcgcggacc gttggatgct ccgacataat aagactttgt atagggcatg
     2641 ctatttggag catcaggata atcaggatgc ttgctaatca agctgataac atccattcgg
     2701 aatccatcaa ttccaagatc aaaccatttt ttcatcattt tataaataga ctgacgaact
     2761 ttcgggttct cccagtttaa atctggttgt tcttttgcaa aacaatgtaa gtaatactgc
     2821 tttctactct caacatactc ccaagccgac ccgccaaatg tcgatcccca attcgtaggt
     2881 gcactcccat cttctttggg atctttccaa atataataat cagaatatgg attttcttta
     2941 tttttttgtg attctataaa ccaagcgtgc tgattacttg tatgattgac gacaaggtct
     3001 aaaataactc gaatattcag ttgatgcgcc tgttccaata atctcttaaa atcttctaac
     3061 gttccataat tttctgaaat tttctcatag tctcgaatat catatccgtt atcaattagc
     3121 ggagagtcaa aaattggagt tagccagata acatcaaccc ctaactttgc taaataaggt
     3181 aatttttcaa cgatccccat aagatctccg atgccatcat tgtttgaatc cttgaaactt
     3241 tgaggataca cttggtagac aacactactt ttccaccatt cattcatccg ttactcatcc
     3301 tcctcatcaa cgactatatt ttttaagtaa tcattgacat aaaccaattg gtcttgtgct
     3361 tcttggattg cctcacggat gtggttgtct gtcaatcttt tgtttaatgt attcaataaa
     3421 attgtcaata ccacaggtag attcatcccg gtaatcagat agacattagg ttgattgata
     3481 tattttataa atgcttgatt gactgaacca cctagcatat cagtaaaaat caatatatca
     3541 gtatcctcat tgtctcttac cattacttcg ttaatctttt cttctatagg tgagttatca
     3601 atataacctg cgattgcagt tatttcacca acattcggta aaatatattg gacagtatct
     3661 ttcaatccta cagctaaatt atgatgacta gcaaaaataa tttttttcat tctttattct
     3721 ccttaaacct ttaaaatacc gaaaaatgat agaatcaatg cgaatatgat cactgaaaaa
     3781 ataatttgaa tgaccgtcat tttttttgaa gctatcaatt tataaacaat aattgttaaa
     3841 actgcaggta agagtgctgg catgatttta tcaagaattt ctgtttgaat aggtaatttt
     3901 acttttccag attgaaaaac cactgcaaca ttcattttta caacagatgg aatcaaagct
     3961 ccaacgactg tcaaacccat aattgaagca gattctgtaa atatcgataa tttacttcct
     4021 aaagcagtca ccaactttaa ccctgaagta tacccaatcg aaaaaagttt aaatcggaag
     4081 aacaaaaaga atagattcaa acataaccaa ataatggcac cagtcggatt tccttctaga
     4141 cccatataag ctgcaatcga gcccataatc gttggataaa ggacccaaac caacgtgtct
     4201 cccacaccag ctaaaggacc catcatccca gtcttaaaac tttgaactgc atctaatgaa
     4261 ctgactccat ctttttcttc cattgctaag ctggcaccta ataataaatt cgccatccaa
     4321 gtcgttgtat tgaaatattt aaaatggtta ttcagagaag ctctgtagtc ttcatcattt
     4381 ggataaattt ttcttagtgc tttttcaagt ccataaacaa ctgccggacc ttgttgattt
     4441 tcgtaattga agatattaca tgccataaac atatagcgaa atgctgctct acgtatatct
     4501 ttctttgata atttatttcc ttcattattc atcaaaatca tctccttcat cgactatatt
     4561 ggcagctaca ggtgatgccg tctctgcttt ttttatttct gtcgtgaaaa agtaatacgc
     4621 taaagcaaag ccgacgattg aaattcccaa aataggcatg tttaaatagg cggaaagtac
     4681 aaaaccaatc aagagcatcg tcatatattt tttcaccggc ataacttgta gaagtaatgc
     4741 tattccgact acaggcaaca tgcttccagc aatcgataat ccatctgtaa accacttagg
     4801 cacaaaatct aaaatcaatc gaacaattct atcaccaaaa acaacacaca atgcagttgg
     4861 aatggctgta gataatccga aaatcactgg tcctaaccac aaaatctgat tcattttctt
     4921 aaaatccatt ttatttgctg ccttttgtgc cgcatgagta acatatgtgt tcaatatttt
     4981 agccaacaca tctaattgaa ttcccagcat cccaacagga acacctactg ctaatgcaac
     5041 agcttttgct tcttcaatat tatttgtggt acggatggct acgtagatac ctacaagagc
     5101 agctaaaccc cagttaggaa cggacgatcc cccaatattc gctacaccca aagccattaa
     5161 ttgaaacgta ccgccaataa ccattgcggt tgtcatatct cccataatca gccctgcaaa
     5221 caaagcaatc atgatgggaa accatgttgt aatgaccaat ccttgttgat ctaatacaac
     5281 ccacactgct aaaataataa ttaaaattcc ttggataatt tccattccaa ttatctcctt
     5341 tattttattc tcacatctaa tggttcttca ggatctgaag gaacaaaatg aaaagtaact
     5401 ggtattccca tagcctctaa tttttttaaa tcagcaattt cttgttcatc aactgaaacc
     5461 atttttttta ctgtttgttt cccttcacca tcaaacatga tcccgatatt cacttttggt
     5521 atatcgatac cgttctcagc tagttttact aatgtactcg gttcttttac gatcataagc
     5581 acattatgac tgtcatactt gccaagtttg aaattaataa tagctgtatc tgtatcaata
     5641 attgacatcc ccgttccagc tggcttactc attcgcattg ctgctttttg tgtgtcatcc
     5701 ttactgactt gatcatctac gaccatcaat cgttttgctt ttgtcgcacc tgcccactgt
     5761 gttaccacaa taccgtgaat caatcgttga tcgattcttg ctaatactaa tcccatatct
     5821 atcgtctcct tttttgttat cgtttacatc ccaaagtata tctacgcctt gttactttaa
     5881 caatagtttt agaagtatca gggaaacagt cgttatctta ggaaatagtt tctttattaa
     5941 gagaaatttc ccacaaaaaa atagcccaac catcgattag atgactgagc tccctaaatt
     6001 tacgctatct tttattacta tttacttctt ttttatcgca atccaaattt cgccatcaca
     6061 atccgtcaac tcttttgaga aatcagtaaa ggagagttct gatgcttgaa ccaactcata
     6121 ttctgaagtt ggcaaccact ccgcgtatat tgacccgctg acatccctaa aaaaggtgcg
     6181 aggtggagaa tatttttgat acaactatcc ttatttatga tatggagccc ccgcattgat
     6241 ccggaaggca cggtagattt gctcgacaag gatcagacgc atcaattgat gtggatacgt
     6301 catttttcca aaagagatgt gagcattgct tcttttcatg acggcttcgc tgagtcctaa
     6361 agagccgccg ataacaaaaa caaggtggct ttttccttgg atacctaatt ggtcgatttg
     6421 tttagcaaaa gattcgcttg tgggattttt cccttcaatt gctaaggcat agacgtattc
     6481 ttgttctttg attttcgcta agatccgttc cccttctttt tcttttactt gtctcatttg
     6541 cgcttcgctt aaattctccg gggctttttc gtcaggaact tcgatcaatt cgatttttgc
     6601 ataggcattc agccgtttga catactcatt gataccttgg atcaggtatt tttcttttaa
     6661 ttttcctact gagataattt tgatattcat gttttctctc ctttggtctt catgctcact
     6721 ttgtttatta taatcaattt tttcgtctat atgtttattt tggctatttt tgttaaaaag
     6781 atttccccaa cagttatgca caacatccac aggtttatcc acagaaaaca gtgttatttt
     6841 accttatttc atcacattta ttaggcaaac atacgtttta taagaattta tccactttat
     6901 acacaggctg tggatatgtt ttatccctta tttttccaat cctatccgcg tagttttaca
     6961 cagtttctgt ggaaaaaagc aaatcaaata ttttcatctg aaaacaatgg tttttcctaa
     7021 agtttttcat aaaaagttca cagtctcaaa gtagactaaa gtcaacaaat aagagtatac
     7081 taacagtaga taaatgaatg taattggagg aggaacacat ggatagaaaa aatgtgacac
     7141 caaagatgaa aaaaaataaa aataatagcc tctggcgtaa gttaggtcta gggcttgttg
     7201 gagggatcgt tggaggatta ctgacagctg gtatttttta tgcagcgatg ggcactggca
     7261 acaatagttc tactacaagt ggcaatcaaa attctgctgg tgaaacagtt gtggaaaacg
     7321 tcaaagtcaa tgtagattct gatatcacca aagcagtaga taaagttcaa gacgctgtcg
     7381 tttctgttat caacttgcaa agtcaaaacc aatcaagcgg ttttggcgga ctattcgggc
     7441 agcaagaaga aagtagcagc tcagatgata gtaatttgga agcttatagt gaaggaagcg
     7501 gcgtgatcta caaaaaagac gggaatactg cttatgtagt cacaaacaac cacgtagtag
     7561 atggccagca aggtcttgaa gtcttgatga aagacggtac gaaggtaaaa gctgaattag
     7621 ttggaacaga tgcttattca gatttagcgg tgttgaagat caattcagac aaagtcgaaa
     7681 cagttgcctc ttttggtgac tcaagtgctc tgaaggttgg tgagcctgcg attgcgattg
     7741 gttccccatt aggatctgaa tatgcgaatt ctgtcacttc aggaatcatt tcttccttga
     7801 accgacaagt aacaagtaca aatgaatcga acgaaacagt caatatcaat gcgatccaaa
     7861 cagatgcagc aatcaaccct ggaaattctg gtggtccttt agtgaatatc gaaggacaag
     7921 ttatcggtat caactcaagt aagatcgcaa gtacttctga atctacttct aatgtaagcg
     7981 tagaaggaat gggatttgct attccaagta acgatgtagt gaatatcatc aatcaactgg
     8041 aaaaagacgg aaaagtgaca cgtccagctc taggtatcac aatggtcgac ttatctgccg
     8101 tttctactca gcaacaagaa caaatcttga agattccttc atctgtaact aacggtgtca
     8161 tcgtgacaaa tgtccaaaca gctacaccgg ctgaaaaagc tggattgaaa aaatacgacg
     8221 tcattactaa gatcgatgac aaagaagttt cttctggtgt agaacttcaa tctgctttgt
     8281 ataagaaaaa agtgggagat tctgtcaaaa tcacttacta tgaaggctcg aaagaaaaaa
     8341 caactactgt tgaattgacg atcgatcaat cggcattaaa acaaaatcaa aatagtgaaa
     8401 attaataaaa aagaggttag gttgtggcaa agacttgtca caacctaacc tcttttttat
     8461 aaactatatt tttcttgcca actcctcggt cacaagtcac aagatccaac aaacatgaga
     8521 agctgttttt tgaaattgct ccttgtgact cagagtgaaa cggcagactc acaacctctt
     8581 tattttggaa atgcttctcc gactacggca tcctcaggca atactaagat tccttttttt
     8641 gctggagcat ttggcaaatg caattcacga gcggaacaga tcattccata gctatctacc
     8701 cctcgcaaag ttcctggcca gatcatcatt ccatctggca tcatagctcc tggttttgca
     8761 acgacaactt tttgtccagc ttcgatattg ggtgcaccgc aaacgatttg caatactgta
     8821 ccattgtcta cttctgtctc agtaatcgat aaatggtcag aatcaggatg tgggacgcat
     8881 gtttttacgt acccgacaac gatcttcggt tcatgatcag cttcaagctg ttcttcgaat
     8941 cctgcttctt tgatcaatcg attcaatttc gctaattcct catctgttaa gcttacttgg
     9001 ccgttccctt cgattgttag atgatcggag atatggaaaa agttccaagc aacaacttga
     9061 ccttgttcat ctttgatccg agcaacattc catttgcgtt caacagtgtt ttctgttcct
     9121 ttatcgtcgg ctataataac catcaaggtg tctccgacgt gtgctttgtt ataagcaaaa
     9181 atcatgttct ttcctccatt atcctttgct gagatttatc ttaacaaaaa attgcattca
     9241 ctgcatgaga tttttttcat gctttatttc cgaagaagcc tcttctggaa caccatttat
     9301 tcgtaacaag ggactgtgac aagacttttg tcacagtccc tttactgttt tagttaattg
     9361 attaaaaagg tcgtacaagt tccacttgct taattacttg gctgttcttc ttcgtcagaa
     9421 acgatcaaga catcacaagg tgaatgacgt atcacgtagc tggctacact tcctgtcata
     9481 aagcgttcga ctgcatttag acctgactgc ccaaccatga ttagatcgat ctcatatttt
     9541 tttggcaatt tgactgccat ggattcacga acagaaccat atgcaataat tccttctata
     9601 tttgtgtaat caacagattt cccatattct ttacattctt cgatcatttc cttggctgtt
     9661 tcagtttgct gatcgactag attttgatca ataggtgcat agcccataaa gttataaaat
     9721 tgctgatcga ttacgtttgc tacatatact ttcccattat tacggcgagc cacttccacc
     9781 gcttttttga aagcagcaag tgcttgttca ctaccgtcga tacctactaa aatgttctta
     9841 tattcttgat tcatcttgac ccctcctaac agatgtcagc cttttgtcac actttctagg
     9901 aacgattcga tttcctgttt tgttttacga tctttattca ctagacgacc taattctttt
     9961 ccgtcttgga tcacgacaaa acttgggata ccaaaaatgt tccattcagc ggctacatct
    10021 atgtacttgt cacggtctac ttcaataaat tgccaatcag aaaagtcatt ttcaatttca
    10081 ggcatttgcg gtttgataaa acggcagtct ccgcaccagc cggcagtaaa gaaaaataca
    10141 ctctttccag tttctacgta gcctgctaat tcttctaaac tatttgggat aatcattctt
    10201 tgtcctctac tttctgttta tgtatttttc aattaaatta tacacctttc tttcgaacta
    10261 acaaacattg atcctctttt tataaaaaaa atctggcaaa agcaatcttt tgccagattt
    10321 tatagtttca ctatcgcatt attttccgta tacgatcgta ttcactgttg atttatccaa
    10381 acctttcagt gtttgtatca acatttctcg tgcagcttca aaatcgcgaa tactgaacat
    10441 cgtttgatgc gtatggatat aacgtcccac tacgccaatc actgtacttg ggattccttc
    10501 gttttgtgta tgagcagcac ctgcatctgt accgcctttt gaaacaaagt attgataagg
    10561 gatattgttt gtttcagcga tatccagcaa gtactcgcgc aaacgaggaa gcatgatcaa
    10621 acctggatct tggatacgca tcaatgtacc ttctcctaaa tgtccgaatg tgccattttt
    10681 cgtcactgta tcatctgctg cagagcagtc tactgcaaag aacagatctg gcttgaattt
    10741 attgacagaa gctttagaac cacgcaagcc tacttcttcc tggacattcg cgcctgcgat
    10801 caatgtatgc cctaatgttt cattttgcaa cgcttctaaa gcttctaata ccattgtaca
    10861 gccatagcgg ttgtcccatg atttgctgat gatattcttt ccgttggctg ttttgatcgt
    10921 ttcagcgtaa ggaacgattg tgtctcctgg aaggacaccg taactcattg cctcttctct
    10981 tgattcaaac ccagcatcga acaaaatatc ggttacttct actgattttt gtccgcttgt
    11041 tcctcgcaat aaatgcgggg gaacggaaga agaaatacat ggataattct taccagtaga
    11101 ggttttaagt gtaaaacgct gtgcagaaac aacgtaagga ttccagccgc ctagaggtac
    11161 gactttaaaa agcccgtttt cttggatttg tgtcagcatg aatccgactt catccatatg
    11221 tgctgcgacc attacacggg gtgcatccgc ttcttcatga tggcgcagac caaaaatgcc
    11281 gcccaaacca tctaactgta actcatctac cagcggttcc atatgacttt ccatataagc
    11341 gcgaacatct tgttcaaaac cgcttgttcc ttgtaattct gttaactctt tgattcgttg
    11401 aaatgttttt tcttccatat gctattcgtc taaatttcag actaaactcc tctcagactt
    11461 atattgatct gcggataaaa tatgattttt tctgcagacc gatcgtattt attatagctt
    11521 attttttctg gtagcggtta tttatttcaa agaaaatatg agagtgtggt aaaataagca
    11581 caaggagtaa caagtgagaa gaaggaggta tgtccacatg tatgaagaaa gcgaactaag
    11641 ctattttaaa ggcgggttag cagttggaat cggtctagga ttattgagcg gggtagcttc
    11701 aacgatttgg tatcatcgca aaaaagtatt gtcagcagac caagtacttg aaaatatcaa
    11761 gtcatctttt ctaaaagaag gtgcaatcga aggttcttgg atctcttttg aaaaggaacc
    11821 tattaggaaa ttcgccgttc ggtcgagtgc ttatcgggga ggtctttctc gaatagaaga
    11881 tggcgaaact gtttattatg aatttttagc agatgctcat acaggaaccg tattagaaat
    11941 ttcaagaaaa aaaggtgcaa cagattaata cgtcaaaaaa gtgagagaac gaatacgcac
    12001 ccactattca aaagagaggt atcctatact tcttttatag tgggtaattt ttataaaaat
    12061 agggctattt cctcttggaa tagctagtat tatagttatt atat
//
LOCUS       DAZABD010000079        11306 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000079 DAZABD010000000
VERSION     DAZABD010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11306
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.080"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(82..591)
                     /locus_tag="ACHALG_002386"
     CDS             complement(82..591)
                     /locus_tag="ACHALG_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587746.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(581..1306)
                     /locus_tag="ACHALG_002387"
     CDS             complement(581..1306)
                     /locus_tag="ACHALG_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587747.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(1529..3649)
                     /locus_tag="ACHALG_002388"
     CDS             complement(1529..3649)
                     /locus_tag="ACHALG_002388"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFN2587748.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            3879..5057
                     /locus_tag="ACHALG_002389"
     CDS             3879..5057
                     /locus_tag="ACHALG_002389"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HFN2587749.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            5073..5831
                     /gene="aroD"
                     /locus_tag="ACHALG_002390"
     CDS             5073..5831
                     /gene="aroD"
                     /locus_tag="ACHALG_002390"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFN2587750.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            5854..6339
                     /gene="ybaK"
                     /locus_tag="ACHALG_002391"
     CDS             5854..6339
                     /gene="ybaK"
                     /locus_tag="ACHALG_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFN2587751.1"
                     /translation="MAKKKIVKRNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(6410..7663)
                     /locus_tag="ACHALG_002392"
     CDS             complement(6410..7663)
                     /locus_tag="ACHALG_002392"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFN2587752.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            7780..9129
                     /gene="gdhA"
                     /locus_tag="ACHALG_002393"
     CDS             7780..9129
                     /gene="gdhA"
                     /locus_tag="ACHALG_002393"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFN2587753.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            9241..10587
                     /locus_tag="ACHALG_002394"
     CDS             9241..10587
                     /locus_tag="ACHALG_002394"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFN2587754.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(10895..11281)
                     /locus_tag="ACHALG_002395"
     CDS             complement(10895..11281)
                     /locus_tag="ACHALG_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFN2587755.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         3683 a         2022 c         2301 g         3300 t
ORIGIN      
        1 aaaaaagagt aaaaaacaag cggtataaaa gatcatgaat cgatcttcta taccgcttgt
       61 ttttactata aaatgattga tttagcccgc gggctctaaa cgatcaatat aaggactttg
      121 agcaatagta gcatcatgag taaagagtaa gataccttga ttttcttcat gagctaaata
      181 agaaagcaag tggagtagct cttggatctc caatacacta agttcatcaa atgtatcttc
      241 aataacaata ttgtttcgct tggaaagcaa ggcatgaagt aattgcagct ttactttttc
      301 aaaagacgtc aactcagctg ccgcacaatt aagcaaggaa ggagagatat caagtttttc
      361 ttgataggcc aaacgatctt gtttgatttc tttttccggt acatccaaca ggacatggtc
      421 tttaagattc agataaggaa acatcggcca atttttttgg atgatcgcaa aattatccag
      481 ttttgcaaca agaacctctt ttttcaagtg ctccaatatt ttatgctgtt tttcgagagg
      541 acacaaaata acggtcaagt gttcagcatg aatcaaatat tcaggtttca ttacgtccag
      601 gcctcctact ttgcaaatga tggataaaaa aagtgaaagg gaatagacta agaatgagac
      661 agcatccact gagcccaata agagtttggg caaatccctt aagtatatct gtgaaaaaaa
      721 gatcattttg gaaatcgatg gtaaaaaaag tctgttgatc catcggaatt gtaagtccgc
      781 ttaccgaagt caagatatca cggatatctg gcagttcagt agtctttaag acaagaaaat
      841 tgatcctttg aagaagcgct tcataaactg actggaagct tacgatccac aataaaacca
      901 agatacaaca aagaaagaga ggaaagaaca gctgccagaa aataaaaagg aataatttgg
      961 ttttagaaag accagtcagg tgccaattga tcaattcctg cttgttttgc ctgactgaat
     1021 agtaaaagaa aaagataaaa aggaaggcaa atagaaaaaa taaactgaat atgcctttta
     1081 tataaaatcg gctagctaat tctaaagccg gaagaagctc tgtagatgca gaagcgccaa
     1141 taaatgaaag ccgatcttgg acttgattgg ctaaagccga ttgcaaatgg taaagagtca
     1201 ggcatgctat caataaaaaa gcagaaaagc ttgaaaataa acaatagagt attgtctgct
     1261 ttttgtaata aattaatcga gcagtagcat agcggattat tttcatactt ttcacctcta
     1321 taaaaagtat agagaatgaa tatgaataaa gtatgaaatt cctttagtga aatcacgaat
     1381 taaaaccaga aaatattttg taaaaagcaa gaggttgtga agaagctgcc ctattctatt
     1441 accgtagatg caagcttttg aacactgttt attcgaaaag aggagcagcg acaataattt
     1501 tgtcgctgct ccttttaaat ttattttatc attcggaaga acttgtggtc gttccgtgaa
     1561 gttcttggta cgtttgcgtt ttataaagat caacagttga ttgtccgttg tgttcactaa
     1621 agacactagt tgacttgtca ccaagagatt tttcagcttc ttttaatttc tctaagccat
     1681 tcttgtagtc ataatcactg gtatcaattg ggtctaatcc actattcgta tagaaccgaa
     1741 gaagatcacc attattgatt tggtcagaaa cagctaactg tttatcaact ttttctttca
     1801 gtgaatcaat ttcattctgt acttcctcag taggttcagt aatagcttca ccagtttcgt
     1861 tactatagat cgtaccaccg tagtaagtat attttggtgt aacaaaatca ccatcacgga
     1921 aagaaacaag ctgttcatga tctttcgaga atagatcttg tcctaactga atataatttt
     1981 ttgtatctac acctagtaag tgaagtaatg taggcaaggc atcaacttgt ccgccgtaag
     2041 tgtgattgat tccgccctgt gtttgtccag ggatatggat catataaggg acacgctgca
     2101 tttgtgcatt gtcgtagttt gtccaagtag aacttgtttt tccaactaat tcagctagat
     2161 ttttatttct tgaattcgag acaccgtaat ggtcaccgta aaggacgatg actgaattat
     2221 catacaagcc gctttctttc aaataattga agaattcttg tacggcggta tctaaataat
     2281 tggctgtcgc aaagtaaccg ttgatcgttt catcatttgt attggctaac gggaatcctg
     2341 cttcgtcatt ggtgaatttt gaataaggat aatggttaga tacagcaatg aattttgaat
     2401 aaaatggttg ttgcagacgt tccaagtatt gtacggattg ttggaaaaat ggtttatcat
     2461 gcaacccgta ttggaacgaa ttgtcatcat taacatcata ataagaagaa tcaaagaaat
     2521 aatcatatcc taaattcttg tatgtctcgt ttcgattcca gaatgtacca gcgttcccat
     2581 ggaataccgc actaatgtaa ccttgtgttt gacttaaaat atcaggtgca gcttggaaag
     2641 tattttttcc gcctaattgt gtgaacaagg agccttgatt caagccaaac aatgaatttt
     2701 ccatcagcgt ttccgcatcg cttgtttttc ctgcttttac ttgatggaaa aagttgtcaa
     2761 aactgaacgt gctgttgctg tggtaaagac tattgatgaa aggtgtaact tcatgctgga
     2821 gaccattctc atctgtcaat ttataatcga tcaggaattg ctggaagctt tctaaatgga
     2881 tataaatcac attttttcct ctagcgagcc cgaacaaatc gtcatttggt gctgcataat
     2941 gttcttttac ataattttct acttctttca tatcattggg acttgcttct gcccgcactt
     3001 ggtttgtctg gtaagtttga acaccgtcat aaaccatgaa agcattcaag ccaagatatt
     3061 tgaccaaata atcacgagag aatgttcgag tcagcaattc tggacgatca gcttccgcca
     3121 taaaaagatt tcctgagaaa atcataactg ctaatgtaga gatcgcaaaa gccattcttg
     3181 ctcggatcgg gcgagggtcc aacttgattt tttttgcaat cagcagaaca gtcaagataa
     3241 taaaatcgat ccaatagatc acatcatatg ggcgaaacag tcgaatggca ctttctccca
     3301 gaccactcgc tactttccca gcacctaaaa tcgtgttgat cgtaatgaag tccgtgaatt
     3361 ctcgataata agtgacattc gaaaaaacaa gcaatgtcaa aagaaaataa atgaccatca
     3421 tcgtgatata agcaactcta gggcgtctga tataaagagc aattccaagt aataacaatg
     3481 tagttgcaat agggttgata aacaagataa agtattgaat gccactttcg attccgagat
     3541 ggaaatcaac taaataggca aaaatcgttt tcaaccaaag taaaacgact agtaaagaaa
     3601 aaaatccgag cctcttattc aaaaaggcag gtgtttttat acatttcaag ataatcttcc
     3661 tttctgcact tcttaatcac agaagtgtcc tgaaaccatg ttacacagct cacttattct
     3721 catttatttt actagtatga caatatagtg tcaattgaaa ccagaagttt atgctgtttc
     3781 attaaggttt tctttaagct tcatgaaaaa atgagcagta agacaagtcc tatgtttttt
     3841 gttatacttg ggaagagaaa gctaaagcag gtgaaggaat gaaagtaaaa gtaaaaaaaa
     3901 gagcaagcaa tcgattgaaa aaaggatatc cactgatcca aaaagaagac cttgctcatc
     3961 cccaaaaaga aaccacgcaa tgggtggaat tagttgattc gcaaaaccaa tttatcgcaa
     4021 aaggatatct aggaaagcag aataagggaa tcggttgggt attgagccag cagaacatcc
     4081 catttgatca atcttttttt gaagaatttt ttgtgaagtc aaaagaaaaa agaactttct
     4141 ttttttcaga tgattctact accgcttttc gtatattcaa tggagaagga gacgggatcg
     4201 gcggtctgat tgttgattat tatgcaggtt atgctgtttt ttcttggtat aacgaaacgt
     4261 tatatgcaca tcgtgaatta ttgattcaag cttttcgtga aatctacccg gaaatcatag
     4321 gaggctatga gaaaattcga tttgaatcag taaatctgcc tgagagccag ttcatttacg
     4381 gaaaagaggc accggaaccc ttgatcgtaa aagaaaacgg agtaaaattt gccgtttatt
     4441 taaatgaagg attaatgact gggatctttt tagaccaaaa agaagtacgg ggatcattag
     4501 cagaaggttt cgcttctggg aaaacagttt tgaacatgtt tagttatacc ggcgcctttt
     4561 ctgttgctgc cgcaatggga ggggcaatcg aaacaacaag tgtcgatcta gcaaaacgca
     4621 gtttaaaaaa aacacaggaa cagtttgaag tgaatggttt ggatgtcgat cagcaaaaaa
     4681 tcattgttat ggacgtattc gagtatttca aatacgccaa aagaaaacaa tttttatatg
     4741 acatgattgt attggatcca ccaagttttg ccagaaataa gaaaaaagtc tttcgcgtag
     4801 caaaagatta tggggaactg gtcaaggata gtctggatat tttagctgaa gacgggatac
     4861 ttattgcgtc tacaaatgct gcgaatgtct cgatagaaca attccaaaca atgattgaag
     4921 aggaattcca tgccaaaaat gtttcttttg aacaacttgc tttatatcgt ctgccaaaag
     4981 atttccagac gacggatacc tttttagaag gcaattatct gaaagtgttt gtttatcaaa
     5041 ttaaaaataa ccattagaag gagcgatgca gaatggttcg ggtaaaggga ttgaatatag
     5101 gaagcggtag accaaagatt tgtgcgcctc ttatcggggc cgtccgagag gaagtattac
     5161 gtgaagcagt tcttgcacgt gaagcagggg ccgatttagt cgaatggcga gtcgatcatt
     5221 atcgtgaggt tttttctttt gatgaagtag cagaaacatt ggttcttatc catgagatgc
     5281 ttgatggact gccgttatta tttacgtttc gtaccttagc tgaaggcgga gaacaagaca
     5341 tatcgatccg tcagtatcgc gacctttatg aacatgtgat acacacagga ttagtagata
     5401 tggtcgatat cgagttgttc aaagtagaaa gtctaggaaa aagactgata gaggaaatca
     5461 agtctttaaa cattccttta attatcagta gtcatgattt taaagaaaca ccagcagacc
     5521 ctgtcttgct ttaccgattg aatgttatgg aacattttgg agccgatatc ggaaaactgg
     5581 cagtaactcc caataatgaa cgagatgttt tgcggttgat ggagttgact agacgggcaa
     5641 atgcttttgt ctccatgcct attattacga tgtccatggg aaatcttggt aaaatttctc
     5701 gtctggttgg cggaacgacg ggttccgtta tgacatttgg ttccttaagc agcgattcta
     5761 cttctgctcc aggacaatta ccggtcaaag cattgaagca aattattcaa ttattaaatg
     5821 aaccgttgta gaacaagttt gaggtgatat tttttggcaa agaaaaaaat agtaaaaagg
     5881 aatgcaatcc gtatggtcga acaaaaaaag attccctata cagagcacga atatgagtgg
     5941 gacgaaagcc acctaagtgc gtcatcggtc gctgaacagc ttccagaaag ccaatctcgt
     6001 atatttaaaa cactggttgc tgtgggcaat gtgacggggc ctcttgttgc tgtgataccg
     6061 ggagaagccg aattgaatct aaaaaaatta gcaaaagttt cagggaataa aaaagtagaa
     6121 atgctccatt taaaagattt ggaagcaacg accggataca tacgtggtgg ctgctcgcct
     6181 ataggtatga aaaaactatt tcctacttat ttagatcaaa ttgcagaaag ctatgaacaa
     6241 atcattgtct cggctggcag aagagggcta cagatggagt tagcacctca agatatctgt
     6301 gcattaacga gcggacaatt tgcagatata aaacaataaa tcatacgtat aaaagaaaga
     6361 aggaggccgg gatgttgttt gtccagtctc cttctttctt ttttatacat tatgctcttt
     6421 gcccggcatt cattcctgct acatgccctg tagcgaaagc cgctgtgata ttaaagccgc
     6481 cagtataacc attgacatcc agtaattctc ccgcaaaata cagaccatca atgattttac
     6541 tctccaatgt tttaggataa atttccttta gagaaacccc tccgccagtt acaaatgatt
     6601 tctcaatagg aaaagttttg ttgattgata gcttaaactc tttgcatagt tgaacaaaat
     6661 cttgaatttg tttttgagag gcttgttgtc ctgtcagatg atccatttca agccgctcaa
     6721 gaaagaaaac taacaatcgc tcaggcaata taccatgcca agcattgacc aagttctttt
     6781 tggttgcttt acttttttct gtcaatatat gaataagctc ttgtttggtt tgtgtaggga
     6841 aacaatccag agaaagtgtc actggttctc ccgttttctc taattcttta ttgacaaaac
     6901 ttgaacaacg cagtgcagcc ggaccagaaa taccgaaatg ggtgaaaagt aaatccatcg
     6961 tatgttcagt caatacacga ccttttttat ttaaaacgcg taaagtgatg tcttgtaaag
     7021 aaagcccttg aagtgttttc tcctgaatat acggttcatc cgatatcaag ggggactctg
     7081 tcgcataaag aggcgtcacc gtgtgaccca ctcgcttcac gatcttgtac ccatctccag
     7141 tagaaccagt agatggataa gtccgtccac cagttgttaa gataatggca ggagcatgga
     7201 actcttcgaa ttccgtgcgt ataccgtaaa ttttatgttc tttgtggatc aatttctcta
     7261 ctcgagtaga gaatagaagc gtcacatcta gttctttcag acgattgagc aatgcttcga
     7321 taatcgtttt tgatttattt gttacaggaa acatccgtcc atgatcctct tcttttaaat
     7381 gtacgccttg tgattcaaag aagttcatga tatcgaaatt attccattgc gcaaacgtac
     7441 tatataaaaa ctttccattt ccaggaatat gtgcaatcaa atcatcaaca ggacgattgt
     7501 tcgtaacatt gcaccgacca ccacctgtca tcagtagttt ttttcctgct tttttgtttt
     7561 tttcgatcaa gagtacgcgt gcgccttgtt cagctgcact gatcgctgcc atcatcccgc
     7621 ttgttccagc gccgactacg atcacatcat acagtttttc cattccctca cctcaaggaa
     7681 agtgtagcat atctgaagaa agaaaacaga aaaaaatgtt ttgagccaaa agagaaaatg
     7741 agctaaaatg agggaagata agcaaaggag cgtggaacga tgacaaacgc aacagaatat
     7801 gttcaagcaa ttcaagaaaa gctacatcaa aaggatcaag ggcaagaaga atttttacaa
     7861 gcaatcgatg aattcatgcc cacggttatg tcttttttgg acactcatcc cgaatatatc
     7921 gaaaagaata tcttagaact tttaacggaa ccagaaagag ttattcaatt tcgagtgcct
     7981 tggcaagatg atgaaggaaa ctggcgagta aatagagggt accgtatcca atacaactca
     8041 gctatcggcc cttataaagg tggattgcgc ttccatccta gtgtcaatct aagtatcttg
     8101 aaatttttgg cttttgaaca gattttcaag aatagtttaa ccggtctgcc aatcggtgga
     8161 ggaaaaggcg gaagtgactt cgatcctaaa ggaaaatctg ataatgagat catgcgtttt
     8221 tgtcaaagtt tcatgctgga attgtcaaaa catatcggcc catcgattga tgttcctgcg
     8281 ggagatatcg gtgtaggagc tagagaaatc ggctatttgt tcggcgaata taaacgattg
     8341 aaaaaatatg atactggtgt gttgacaggt aaacctttgg atttttgggg aagtaagata
     8401 cgtacggaag ctactggtta tggattagtg tattatgtca aacatctatt gaacgaagaa
     8461 aaagattcat ttaatcaaaa aacagttttt gtttccggaa gcgggaatgt agccatttat
     8521 gcaatcgaaa aagtgcaaga gctaggcggg aaagttgtta catgttcaga ttctagcggg
     8581 tacatctatg atccagaagg aatagatgtg aaactattga aagaagtcaa agaaatcaat
     8641 agacaacgtt tgacggaata cgcaaaccaa cgtccttctg ctatttatca tgctggagaa
     8701 tctgtttgga cattgaaaga aaaagcggat atcgctttgc catgtgctac tcaaaatgaa
     8761 atagacgaaa gcttggccga aatcttagta gaaaatggga tcaaaatcgt agcagaaggc
     8821 gctaatatgc catgtacagt aaaagctgtt tctgttcttc atgaagcagg cgtgtggtat
     8881 tgtccaggaa aagctgctaa tgctggagga gtagcagtat ctgctttaga aatgagtcag
     8941 aacgtccagc gattgacttg ggaaagagag caagtggatg agcagctgga tcaaatcatg
     9001 gaaacgattt accatacgtg tcgccaaaca gcagccgagt acggcgacga aaaaaatctg
     9061 cttcttggag caaatgttgc tggatttgaa aaagttgcca aagcaatggc agcagaaggt
     9121 ttagtttgaa aaatgaaact ttcttggaaa tattgtcagc attttgttta tttgcttgaa
     9181 aaataataaa aaaaaacgta aagtattacc tgtagagtaa acctataagg aggagaacaa
     9241 atggcacaca ttcattttga ctattcaaaa gtagcaccgt ttgtcaacga acaagagtta
     9301 gggtacatgc aaagccaagt aacagcagcc cacaacgaat tacgggaagg tactggcgca
     9361 ggaagcgact tccgcggctg gatcgattta ccaacgaact atgacaaaga agaatttgca
     9421 cgtatcaaag aagcagctaa aaaaatccaa tctgattctg aagtattagt tgttatcggt
     9481 atcggtggtt cttatttagg cgctcgtgca gcgatcgact tcttgaatca cacattcttc
     9541 aatttattag atagcaacac aagaaaagca ccacaaatct tctttgccgg aaattcaatc
     9601 agctcaacat atattgctga cttgatcgaa gtaatcggtg atcgcgactt ttcagtaaac
     9661 gttatttcaa aatctggaac aactactgaa ccagcgattg cgttccgtgt attcaaagag
     9721 cttcttgtta agaaatatgg acaagaagaa gcaaacaaac gtatctacgc tacaacagat
     9781 aaagcaaaag gtgcagttaa agtagaagca gatgcagaag gttgggaaac tttcgtgatt
     9841 cctgatgaca tcggcggacg tttctctgta ttaactgctg ttggtttgtt accaatcgct
     9901 gttagtggtg cagatatcga tgcattgatg caaggtgccg ctgatgcaag caaagcatac
     9961 tcaagcgaca aactagaaga aaacgaagct taccaatacg cagcaatgcg caacattctt
    10021 taccgtaaag gcaaagtaac tgagttgttg atcaactatg aaccaggaat gcaatacttc
    10081 tcagaatggt ggaaacaatt gtatggtgaa tctgaaggaa aagaccaaaa aggcatctat
    10141 ccttcaagcg caaacttctc aactgatttg cactctttag gacaattcat ccaagaagga
    10201 cgtcgcaata tttttgaaac agttgtgaag gttgaaaaac ctcgtaaatc aatcacaatt
    10261 cctgaacaag cagaagattt agacggtcta ggctacttgc aaggaaaaga agtggacttc
    10321 gtaaatacaa aagcatttga aggaacattg cttgctcata cagacggtga cgtgcctaac
    10381 ttgctagtca aaattccaga aatggatgct tatacattgg gttatacaat gtacttcttt
    10441 gaaattgctg taggtatttc aggttacttg aatggaatca acccattcga ccaaccaggt
    10501 gtagaagctt acaagaaaaa catgttcgca cttctaggca aaccaggctt tgaagatctt
    10561 gctaaagaat tgaacgaacg cctgtaagat aagggttttt cgctagattt aaataatcag
    10621 tgatagaaac atgttcgctt taaaatgaaa actggtaccc atttaggtac tcaaacatac
    10681 tgcatcgttt attcagaata aggggctgtg acaagacgtt tgtcacagtc ccttgtttgt
    10741 atagaaatcg attgattgcc ttgctcttta gtttgtttta ggactttaag aacagtgagg
    10801 ctgacttcga aggttaattc tgtttttttg ttttctagaa tcagtaagca aaaaagcact
    10861 cacttcgagt gcttttttgc gatatattct tagcttaaga taggcctaat ttttctttta
    10921 cagatgaggg taccttatcg ctggtaatta cttttttctc tatgatccgc ttttcgcttg
    10981 ctttcaactg caagatcgtt ccttcggggt aagcagaacc attggcagag tcaccagaag
    11041 taatgacatc gaaatctaac tgtctttttt caccgttttc agcataagca tctacgttgt
    11101 atttaaaacc tcttccaatt acttccccat tatctgcttt gatatcagtt tctgcaggca
    11161 ttggagcttt gactaccgca taataatctt tacctacata agtagactga tagtagtccc
    11221 aacctttcca tcctataaaa gcaatcaata tgataacgat caaccctaaa atttttttca
    11281 tgaaagatct ccttttttat ttttat
//
LOCUS       DAZABD010000080        11207 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000080 DAZABD010000000
VERSION     DAZABD010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11207
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.081"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1603
                     /locus_tag="ACHALG_002396"
     CDS             <1..1603
                     /locus_tag="ACHALG_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFN2587756.1"
                     /translation="LKSKGYDLDFLKQVMPQGGLNFDEEYVTYGNGYSRCVTVYHYTK
                     NPTLFWLANLMNNPHTVATLDVSTDEKQKVIDQINRALDELNDRSDNERKATDRNKSA
                     EEYLELAEYARKLTSNGEISKQVKVRVHVYGNTIQEVDERAQELIENLKGMDYRAVTY
                     LHKTKRDWQSMFTMFDEQAEWFGNIKGQSLPSLNIGGGFPFHHQTLKDPGGTQYGETM
                     TGGPFVWNATYKNSVRLSTNTLLLGMMGAGKSTVLKMIAEAHLAAGDFIWGFEKGKDF
                     IPFLKEYNGIMVRLDGSDGMINPLEIFATRTYDEASSLYDDGSVKDLKINEAASYQTH
                     LDKVVYQVQLVSPQLKGTMKAEFKTYLNRFYEEYGTVPRGFTSSNSRSNTDTQVTGKD
                     PEAYPTFKEFLAFLGQLELPGASQEKKNRKEEMESIVESLCETYGMIFDGHSTIRHLD
                     QQQLVFFDIDSITGLDRGVQQCQMYMAMTLIWNQALIQGRRQRRLIKEKQMEEQDFKR
                     FLAMIDECHNGATCSAVKSCGLQVV"
     gene            2291..4168
                     /gene="ltrA"
                     /locus_tag="ACHALG_002397"
     CDS             2291..4168
                     /gene="ltrA"
                     /locus_tag="ACHALG_002397"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFN2587757.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            4299..4676
                     /locus_tag="ACHALG_002398"
                     /pseudo
     CDS             4299..4676
                     /locus_tag="ACHALG_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     gene            4676..5881
                     /locus_tag="ACHALG_002399"
     CDS             4676..5881
                     /locus_tag="ACHALG_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFN2587758.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDP
                     LPFLNSSKK"
     gene            5897..6541
                     /locus_tag="ACHALG_002400"
     CDS             5897..6541
                     /locus_tag="ACHALG_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587759.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQAANTYKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPTDDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVIDFSYFTR"
     gene            6552..7358
                     /locus_tag="ACHALG_002401"
     CDS             6552..7358
                     /locus_tag="ACHALG_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587760.1"
                     /translation="MSKKKGERQRFKKRKSYLRWLGIFMILGFLILASPIYTNQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIRAKVIKVSDDYFAVKLTHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQLKDQEEIIQKLQEKIDRMTKEKI"
     gene            7380..7610
                     /locus_tag="ACHALG_002402"
     CDS             7380..7610
                     /locus_tag="ACHALG_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587761.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
     gene            8167..8442
                     /locus_tag="ACHALG_002403"
     CDS             8167..8442
                     /locus_tag="ACHALG_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587762.1"
                     /translation="MEVKIRDIDEKTIQRIDRRCIELSERTGTKWSRNRYLKVLIEND
                     FDHELMDHRKDQFDRLLEKFVDIQTYNTKILEEYIATNNQLIALLIE"
     gene            8484..8906
                     /locus_tag="ACHALG_002404"
     CDS             8484..8906
                     /locus_tag="ACHALG_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587763.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAKERVE
                     GKIKANQTKKYSPKIKKPINESVHARVDPFDPTKFLKR"
     gene            8926..10518
                     /gene="mobP2"
                     /locus_tag="ACHALG_002405"
     CDS             8926..10518
                     /gene="mobP2"
                     /locus_tag="ACHALG_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobP2 family relaxase"
                     /protein_id="HFN2587764.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEKERRTPDE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEVENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPDKRSEWHYGDEKRTKLSPYTREKLDELTKYVLTKEPEYQEYIQ
                     ISKSLKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKKQDSQIKPLIESMD
                     QMQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            10539..10874
                     /locus_tag="ACHALG_002406"
     CDS             10539..10874
                     /locus_tag="ACHALG_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587765.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPDCLEKEHLFQIREMLRNEQF
                     DPNQFLVVEYSATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEMNEEGINNFPCKSI
                     EESISLTEC"
     gene            11020..>11207
                     /locus_tag="ACHALG_002407"
     CDS             11020..>11207
                     /locus_tag="ACHALG_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587766.1"
                     /translation="MAYSTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQSGDI
                     LTLNLTTKTFVDAAEDYP"
BASE COUNT         4210 a         1764 c         2371 g         2862 t
ORIGIN      
        1 tttaaaatca aaaggttatg atttagattt cttaaaacaa gtgatgccac aaggtgggtt
       61 gaattttgat gaagaatatg tgacctatgg aaatggttat tcacgatgtg tgacagttta
      121 tcattacaca aaaaatccta ctcttttttg gttggccaat ctcatgaaca atcctcatac
      181 agtggccaca ctagatgtta gcacagatga aaagcaaaaa gtgattgatc aaatcaaccg
      241 cgcattagat gaattaaatg atcgatcaga taatgaacga aaagcgactg accgaaataa
      301 aagtgcggaa gaatacctgg aactagcgga atatgcccga aaattgactt ctaatgggga
      361 aatttctaaa caagtcaaag tgagagtaca tgtttacgga aatacgatcc aagaagtgga
      421 tgaacgggcg caggaactga tcgaaaatct aaaagggatg gattatcgtg cggtgactta
      481 tctgcataaa accaaacgag attggcaatc gatgtttacc atgttcgatg aacaagctga
      541 atggtttgga aatatcaaag ggcaatcact tccatcgtta aacattggcg gaggattccc
      601 gttccaccac caaacgctaa aagatccagg aggcacacaa tatggggaaa ccatgacagg
      661 cggcccattt gtctggaatg ccacgtataa aaatagtgta cgtttatcta ccaatacctt
      721 gcttctagga atgatgggag ctggtaaatc gaccgttctt aaaatgatcg cggaagccca
      781 tttagctgca ggagacttta tttggggctt tgaaaaggga aaagacttta ttcctttctt
      841 aaaggaatat aacggtatta tggtgcgttt agacggatct gacggaatga tcaatccttt
      901 agagatattt gccacacgga cgtatgatga agcatccagt ttatatgacg atggatcagt
      961 caaagatttg aagatcaatg aagcagcgtc ctatcaaaca cacttagaca aagtggtcta
     1021 tcaggtacag ttagtgagtc ctcagctaaa aggaacgatg aaagcagaat ttaagaccta
     1081 cttaaatcgt ttttatgaag agtatggaac agtaccacgc ggctttactt cctcaaattc
     1141 gcgttcaaat actgatacgc aagtcacagg gaaagatcca gaagcttatc cgacgttcaa
     1201 agaattttta gcctttttag gacaactgga actaccaggg gcttcccaag agaaaaagaa
     1261 tcggaaagaa gaaatggagt ctatcgtgga atccttatgt gagacttatg gcatgatttt
     1321 tgatggtcat agtacgattc gacaccttga ccaacaacag ttggtctttt ttgacattga
     1381 ctctattaca ggcctagatc gaggggtaca acaatgtcag atgtacatgg ccatgacgtt
     1441 gatttggaat caggcattga ttcaaggacg aagacaacga cgcttaatta aagaaaaaca
     1501 aatggaagaa caagacttca aacgcttctt ggcgatgatt gatgagtgcc ataatggtgc
     1561 gacctgttct gcagtgaaaa gctgtgggtt gcaagtagtc taaaaactat tgtaataact
     1621 cttataacga gaagtcaaat gagtgggtaa cgccacaaag ggcttccact gatactccga
     1681 catgcaacaa gtagtttatg cagaccaacg ttgtgtgaag ctcggtgaag tcggctgaag
     1741 gataccgtaa ggtaaaagaa atagtgaata ccacgaaagt tctctagagg taaagggtgt
     1801 atccgataag tcgggtgtcc aaaaaaactt aatatggtga gtatgtacaa acgtactgac
     1861 gaactagcga atgtactgtc cgaaaggcat agctcctaga aatgggagag acagtgagca
     1921 aaaaatcact cattgtgcta acttgtggtt gagtaaaagt cgttgttatg aaagaccatg
     1981 ctgcgttaca ggcgtaggag aagttagggg gcaaaagcta gcatctaagt taaggcatgt
     2041 ggatagatat aagggaacag gggaagccat caacatggag agtgcatact cgatgaagtg
     2101 gtagtaagaa atatgtgaat gacaaataac ttactttagt gatggtgaaa gtagagtcac
     2161 gactgatgaa ggttctgtaa tggaactgga ggaacaggct cgattcgtct gtaaaagtca
     2221 tgaaacccgt tcaaccatgg gattctcgta tgactaaaaa ggtcacgagc caattggagc
     2281 gtgataaacc atgacaaaag aaacaagtcc aaaagatagg aagttgcggc atgcagaata
     2341 ttatggcatg acggaaatct tcgataagat atactccaaa agtttgaagg gagataattt
     2401 caagaatcta atgcagatta tcatttctga taacaacatt gtcctagcat atcgtaatat
     2461 caagagaaat cacggtagtg tgacaagagg agaagatgga gtaacgatta aagatattga
     2521 aaatctcaac cgtaatgaat tcgtagaaat tgttaggaag cgattcgcac attataaccc
     2581 tcggaaagta aggcgtgtag aaattcctaa atataatggt aaattaagac cgttgggtat
     2641 tccatcaata tgggatagaa tcgcgcagca atgcatctta caagtattag aaccaatatg
     2701 tgaagcaaaa ttcaataatc acagctatgg tttccgccca aatagatcag cagaacacgc
     2761 aatagcagat tgtatggccc gaatgaatca aagtcacatg gaatatgtgg tagatgtaga
     2821 tatcaaaggt ttcttcgacg aagtcaatca tactaaactc atgagacaaa tctggtctat
     2881 gggtatacga gacaaacagc ttctcttaat cattaggaaa atgttaagag cacccgtagt
     2941 acttccaaat gggaagttga tatacccaac aaaaggaaca ccacaaggcg gaatacttag
     3001 cccattactt gcgaacatca atctcaacga atttgactgg tggatagcaa atcagtggga
     3061 ggaaaaagat gccaaagagc taaaacatca ttttaggaaa aatggagctt gggataaagg
     3121 taatatctat cacaagctca gaaagagtac aactctaaaa gaaatatacc tagtgagata
     3181 cgcagatgat tttaaaattt tctgtagaaa ttataatgaa gcgaaaaaag ttttctatgc
     3241 aactaaaatg tggttacagg agcgtctaaa gttaccaata tctgaagaaa agtcaaaaat
     3301 cacaaatctt tcaaaagaag aaagtgaatt tttaggcttt acgatgaaat tagtgaccaa
     3361 aggtaacaaa aaggttgtta atacgcatat aggtacaaaa gcacaaagca tgattaaagt
     3421 gaaactaaaa aaacaaatca aacgaattca aaaaagtcct aatagcaaag atacctttat
     3481 ctatattgga gtctataaca gtatggttat tgggattcat aattacttca aaattgcgac
     3541 tcattgtagt gtagcaatgc gaaaaattgc aagagaactc agaccaagtt ttgaaatacg
     3601 actacgagaa caaggaatct caaaagaagg acaatacgga aagtacgaag gatacaagcc
     3661 ttacctacgg tcaggacaaa tacgatatat aagagggttt ccaatattac cagtagggta
     3721 tattcaacac agacatccga tgtgtaaaaa aagaaatatt aataaataca ccgaagaagg
     3781 tagggaagcc attcataaga agcaacaaac cgtagaagaa tggaagatat cgtggttgtg
     3841 tagcaatcca gtgcttagta agagagcaac gattgaattt gcggacaatc gaatttcaaa
     3901 attcatcgct caaaagggaa gatgtgcagt aacaggtgag gagcttatct tatctgaaat
     3961 gcattgtcat catattatcc cttatcatga aagcaaaagt gatagctatg aaaacctagt
     4021 gattgttact gaggaggttc atcgggtaat acacgctaca caaagtgaaa cgattgagga
     4081 acttctaaaa tatctaaaat taaatccaaa acaaaaagaa aaactcaatg agttgagatt
     4141 aaaagttggt aacgaagaaa tatcttaata aatatttgta ggacgggaaa acagaatgtc
     4201 ttttatagac gagggaacgc cgtatgcggt gaaagtcgca cgtacggtgt ggactggggg
     4261 aaaagacgga gataatatca aactcttacc tatcagtatc gattaatcca gattttattg
     4321 aaacggtaaa ttatgtcaca aactttcaac gagaaatgcg taaagtagaa gcaatgactg
     4381 ttttagctac acaatcccca caagaaatgg tgccagaaaa tatgtcttct gataacttct
     4441 cgaaactgaa gaaagtcttt gaattttctt ctacgaagat ctttatgcgt atggatgaat
     4501 ctattttacg tcatatagaa ggattagtgg gtaaatcaat gacgaactca gaattagaat
     4561 ccattcctca acaaaatcaa ggagacgctg tgatcaattt tggctcaaaa gaaaccattc
     4621 gtgttcattt tagtccgaac aaacgtcaat taaaattgtt tgatggagga aaataatgga
     4681 aaaccaaaat gaaagtttaa tcaagcagta tgttaagcgt cgtgcaaagc ggcgcttatt
     4741 tttgtggttg tttggtactt ctgcaggttt gattactatt ttgatcactg tgttcgtgac
     4801 cctatttcta atacttgcgg cgggctcgat tgataattcg gattcagatt caagcagtgg
     4861 tggcgaagct tttacaggag aatattcgga aggcttaccg atttataaag aaatcaaagg
     4921 aagaggccct ttttctgatg agattgcgca atatgccgta ggtgcagcag tgaaatataa
     4981 attattacca agtgtgatcc tttcgcaata cggttacgaa tcagcgtttg gtacatcggc
     5041 gtccgcaaga aatgatctca actattttgg gattacttgg tttgatggtt gtctgtttcc
     5101 taaaggaact gcacgaggaa ttggtggcat tgaaggtggc tggtacatga agtttcccaa
     5161 ctcaaaagct gcattttcct attacggctt tatggtagcc acacaatcca attttaatgc
     5221 ttgtgtagga aataaaagcc ctggcgcaag cttattgatc ttaggacgag gcggttatgc
     5281 cgctgcagga attacggaag atagtccgta ttatacaggt tgtatgtcaa ttatcacttc
     5341 taataaatta accgaatatg atgaattcgc catcaaacat tggggtgaag gtggcaataa
     5401 caacggcacg attactggtg aatggacgaa tccattccct ggcagcagtt tagataaaag
     5461 ttccttcagc ggtggtcaat tatttggaac gaatccaggc ggtgaattcc gtccgaacgg
     5521 cttccatgat ggattagatt ttggttcagt tgatcatcca ggaagtgaga ttcatgcggt
     5581 tcatggcgga aaagtcgtgt acgttggcaa tcctggtatt tcaggtttgg gcgcgtgtgt
     5641 gatcgtgatt aattacgatg gcttgaatat ggtgtatcaa gaatttgcaa atagtacagg
     5701 aaattctcga gtgaaagtag gcgatcaagt gaaagttggt caagtaattg ggatacgaga
     5761 tacagcacac ttgcatttag gatttactcg aatggattgg cgtcaagcac aaggacatgc
     5821 attcattgat gatggtacat ggatcgatcc gttaccattt ttgaatagca gtaaaaaata
     5881 gatcaggaga aaaaatatgg aaaataaaac gttaaaaatc gtcttaacag ctgcaattag
     5941 cgttagttta atcggcaata ttgcgttgtt ttggcagctg aaagaggaac aagcagcaaa
     6001 tacctataaa attgaagaag tggctaaaaa cgcgtctgag aagctcaaaa aaaattcctc
     6061 tgaggcacag gcgaagatcg acaagttaaa tcaaaccatc aatgagaaaa accaagaaat
     6121 cgatcagctg aagcaacaag atggcacaaa agcaaaagag gaagtaactg aagcacaaag
     6181 aaagacggca gaagagttcg gtcaattggc cattgataaa tcattaagca gaaatgaatt
     6241 aacggaaaaa ctaaaagatg tcgcaaccca agaagtgatc gacaaattaa gtcctacaga
     6301 cgatgatcac caacatgatg attacggaag ttactccttt acattaggtg acgtgcaaac
     6361 ttacgctcaa acaagcagtg taaaagagga acaaaatttt gtcgtatttg ttgattatac
     6421 aatcggcaat ccgcaattca aagatgtcaa accacaaaaa ataaaaggtg gattaactgt
     6481 cactgaaaaa caagtagatg gtcaatggaa agtcatagac tttagttatt tcactcgtta
     6541 ggaggaagaa aatgagtaag aaaaaaggag aaagacaacg tttcaaaaaa cgaaaaagtt
     6601 atcttagatg gctgggaatc tttatgattc tcggcttttt gattcttgct agtccgattt
     6661 atacgaatca gaaggtagat ataccagatg aggtaagcac gaacgccttt ttaaacacga
     6721 aagagctgac aatggtttca aaagaaaaag gagcgaacca gcaattagtt tttgaatttg
     6781 cgatcgatga tgaagatcaa agcttgttaa aagaactggc taatcttaat tatcgcgtag
     6841 aagtaaagac agctaaaggg gattatcaag caatacgagc caaagtgatt aaggtgtctg
     6901 atgattattt tgcagtgaag ctaacgcatg taccagaaga atatatcgcc atgcgtttaa
     6961 ccattattcc tgaaaaaatt gatccaaaag tggatatgca agaaccacaa gatctgattt
     7021 attacgtgca cgaagataag gttaaagatc aagtgaaaga tgaagattat gaacaacacg
     7081 cgatcaatta caaagtgaaa ggatacaaga aagaacaaaa agcagctcaa aaacaaatgg
     7141 aatctttcca agcaaccatt gatttgaatg aagaattagt gaagaagcta aaagatcaat
     7201 tgccttatca agtcgcagat gatcaggaaa atactgaaaa caagattaat agctaccagc
     7261 aagaaattga aacatcgaaa acgcaactga aagatcaaga agagatcatt caaaaacttc
     7321 aagaaaaaat tgatcgtatg acaaaagaaa agatttagaa ggacaggtgg agtgaaaaaa
     7381 tgaagcgaac aaacttggct gacaaagata ctttcgctga gcagaaagta ttgattaaag
     7441 cctatctcaa agttgtaaaa gctaaagaaa tatgtcgaga agtgaattta tctaacacaa
     7501 caatatctga gttgaagaat ggtcgaaaga atttagatac tttaaaattc agatcatttg
     7561 aagctctata tgtagctgct aaaaaatatt ttacagctga taacaaataa aaaacaggag
     7621 tattttatgt gaaaaaagta tataaaaaat gcttatgaat cagctatatc accttaacca
     7681 tttgttaatg agatgggaga aaacattaac cacgattcta acgcaaacat caagcacata
     7741 aaacatttaa cactcctcct ttctggctga agatttcaac caaacaggaa gacagcgtat
     7801 caattatagc atacactttt tcttaaattc atttttcata tgacaaaata tttccttcta
     7861 atcgacaaag ttagaaggat tttttattgt ctaaatacac aaataacctt aatttttaaa
     7921 gagcataagg aaacgtgtac ccactttcaa aataaagtgg gtagataggc tgtgcctatc
     7981 acgtttctga aaaaaataaa tgaccccaaa attttcaatt tatgtgtagg tttatgggta
     8041 gcaaaaatat gaatgtgggc agcaaaaagt tcatttgtgg gtagtaaaat tcaaatgtgt
     8101 gtagcaatgt gtgtagctac acacattgct acacacgcta taacatatag tgaaaaggag
     8161 atatttatgg aagtaaaaat aagagatatt gatgaaaaaa cgattcaaag aattgaccga
     8221 agatgtattg aattaagtga acgcaccggg actaaatgga gccgaaatcg atacctaaaa
     8281 gttttgattg aaaatgattt tgaccatgaa cttatggatc atagaaaaga tcaatttgat
     8341 cggttgttag aaaaatttgt ggatattcaa acttataata cgaaaatttt agaagaatat
     8401 attgcaacaa ataatcaatt aatcgccttg ttaattgaat aataaaaata gtattgtgaa
     8461 tttataaaag gaggaaatca attttggcaa aagcaacaag aaaacatgta tatctttacg
     8521 atatgcatat cgaagtactt gatgaaataa tttctactca tgaagatata tctaattata
     8581 gtgaagcagt cagacatttg tgtatgatgt atgatccaaa aatcgaagaa aaaataaatc
     8641 caattcaaat taaattgaat gcaatgggaa aagaaatttc tatattaacc gaaatggttt
     8701 cagagtttgc ggatcttcat ttgaaagata caggaatact aattggtaca aattcaactg
     8761 tctatcaaga agcaaaagag cgtgtggaag ggaaaataaa agcgaatcaa acgaaaaagt
     8821 attctcctaa gatcaagaaa ccaataaatg aatctgttca tgccagagta gacccgtttg
     8881 atccaacaaa gtttttaaaa agataaaaag tagaagggga acatgatgaa taaatcgcct
     8941 gcagtcattc tcacttgtca atttaccttg cccaataaaa aatctttttc aacatacatt
     9001 gattatatga caagagaaaa agcgctggaa gaaaaggaac gacgcacgcc ggacgaagaa
     9061 caggaactgc aacgaatcaa gaatgcttta gaggattttt atattcctga aggagaaact
     9121 ttttcggaga gtaagacaga taaagaattc agtgaaacgt cgaatgaagc tcaatcgttg
     9181 atggaacatg gaatcaattt tgacaagtta gaggaacaag attttaccaa atatctttct
     9241 tatatgaccc gtcactatgc gttagaacaa aagaaagaac tgacgcaaag ggaacaaaaa
     9301 gaataccaac ggttgaacca agcaatgaaa aaatatcaaa cagagccaca taaaaaagct
     9361 tcaggaaatc ttcctggagt tttttctatg gcttcagacg aagtgaaagg aagtgatctg
     9421 aaggagatca aacaaatctt ccaaagaggg caacaaaaag gctcaatcat ttatcaagat
     9481 gttgtctctc acgacaattc gtatcttgaa aaaatgggct tgtacgatcc caaaacagat
     9541 accttagatg aagaaggatt aaaagcagct ggaagaaaaa tgatggagac attattcgag
     9601 aaagaacaat tgaatgaaac aggttactgg atggcaacga ttcaccgtaa cactaagcat
     9661 atccatattc attttgcagt tgttgaaaaa gaaaatacgc gtaaacctta cacggaggtt
     9721 gaaaatggtg tgagttatat cgtaccgaaa ggtaagagaa gacaagcaac attagacgca
     9781 atgaagacca attatgtaca tgagttagaa cgtttttctt atgaaaagga tcgaatgatt
     9841 ggtatggaga aacgaaattt attgacgcgg aaaagtgatt tgcgtaacac cttaacaaaa
     9901 gtggtaaaag agccagcgcg ttacgatcga cacgcaatga acttattaaa tgaagtatat
     9961 aaaaccctac ctgacaaacg ttcggaatgg cattatgggg atgaaaaacg aaccaaactt
    10021 tcaccataca caagagagaa actcgatgag ttaaccaaat atgtgttaac aaaagaacct
    10081 gaatatcaag aatatataca gatttcaaaa tcattgaaag aagaagcaaa aagtctttat
    10141 ggggaaagca agagagaatc aaaggatgca gaaaaaaatg ctctctttga tttgaagaag
    10201 cgcacaggaa atgccattct aacagaactg aagaaacagg atagtcaaat aaaaccttta
    10261 atagaaagta tggatcagat gcaatctatt tacaatgaac gatcctttcg aacgcttaaa
    10321 cagttaccaa cgaatgaata caaatatagt tacgaagaat tcatgaagcg caggaaacaa
    10381 ttccaacaaa gagttgtcac gcaacggagt ttgcgtcaat taaatcgaac gattaatgcc
    10441 catgaagaaa aataccacgc aagtaaagcc tacgaagagc aacaacgaag aattcaaatg
    10501 gaacaagaaa gacagtaaag acagtaggag gaatagcaat gaaacaatat ttagatcaat
    10561 ggaaagttat agaaggatca ttaagagaag aacgaatcga acaacttccc gattgtttag
    10621 aaaaagagca tttgtttcag ataagagaaa tgttgagaaa cgaacagttt gatccaaatc
    10681 aatttttagt cgttgagtac tcagctactg gtgtgtactg ttgtaatcat gtgaagggtg
    10741 aaaaatattt tatcattcaa gaatatgagg gcaagttagc gccttattat actacttggg
    10801 aaatgaatga agaggggatc aataacttcc cgtgtaaaag cattgaagag tctatttcac
    10861 tcacagaatg ttagttgtta agtgaataaa agaagtaaaa gagtcctgaa cgtaattgtc
    10921 caggactctt tttttcaatg aattgttttc cgccaattca atgagtggtg cgatttgcga
    10981 ctacattata tcaaaagaaa gagaagggag gaagaagaaa tggcttattc aacgattgga
    11041 gttgttgttg aaaaaagcag agacaatctc gtttttgtga cagaaattca aacgggcaga
    11101 gcttttgtcg tcacagacaa agcggcaaaa gcgtatcaaa gtggcgatat tttaacgtta
    11161 aatctgacta cgaagacgtt cgtcgatgcg gcagaagatt atccatt
//
LOCUS       DAZABD010000081        11150 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000081 DAZABD010000000
VERSION     DAZABD010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11150
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.082"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..305
                     /locus_tag="ACHALG_002408"
     CDS             6..305
                     /locus_tag="ACHALG_002408"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFN2587767.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="ACHALG_002409"
     CDS             378..1739
                     /locus_tag="ACHALG_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFN2587768.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFLSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLLFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGILTMPIYLYGATYLAPILSDSAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="ACHALG_002410"
     CDS             1761..2021
                     /locus_tag="ACHALG_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587769.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="ACHALG_002411"
     CDS             2035..2787
                     /locus_tag="ACHALG_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HFN2587770.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="ACHALG_002412"
     CDS             complement(2881..3486)
                     /locus_tag="ACHALG_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFN2587771.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIEQAREWTDHLLTK"
     gene            complement(3542..4516)
                     /locus_tag="ACHALG_002413"
     CDS             complement(3542..4516)
                     /locus_tag="ACHALG_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HFN2587772.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DCPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4894..5613)
                     /locus_tag="ACHALG_002414"
     CDS             complement(4894..5613)
                     /locus_tag="ACHALG_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFN2587773.1"
                     /translation="MPKYEEIANILRERIRSKEYPPDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5740..7011)
                     /gene="celB"
                     /locus_tag="ACHALG_002415"
     CDS             complement(5740..7011)
                     /gene="celB"
                     /locus_tag="ACHALG_002415"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0019191 - cellobiose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFN2587774.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7164..8561
                     /locus_tag="ACHALG_002416"
     CDS             7164..8561
                     /locus_tag="ACHALG_002416"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFN2587775.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWNHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSRLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKAIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGTIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8642..9082
                     /locus_tag="ACHALG_002417"
     CDS             8642..9082
                     /locus_tag="ACHALG_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFN2587776.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9229..9555
                     /locus_tag="ACHALG_002418"
     CDS             9229..9555
                     /locus_tag="ACHALG_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFN2587777.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9556..10305
                     /locus_tag="ACHALG_002419"
     CDS             9556..10305
                     /locus_tag="ACHALG_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HFN2587778.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10331..10741)
                     /locus_tag="ACHALG_002420"
     CDS             complement(10331..10741)
                     /locus_tag="ACHALG_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFN2587779.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNK"
BASE COUNT         3538 a         1948 c         2291 g         3373 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttcgaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcctgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtctta ttgctgcttt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattacca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcctc acgatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgattcagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaaggaccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcaaaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgggagat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc tcgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccaatc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa aaatgaagta gcatgaagag
     3541 attattcacg ttttttattt gcatcacttg tatcgaaagg acggacatag gcttcaatgg
     3601 cttcacgacg ttcttctaag aatggaggaa gagatagttt ttctcctgct gattctgctg
     3661 gttcatcttc aaggaatcca ggacaatcag ttgctaattc aaacagtacg tgatgagcgg
     3721 ctaggaagta ctcagattgg aaatagaaac gatcgacgaa accagaattt ggcaattcaa
     3781 tcttattgat tacttgaatc cagtattcca atgcttcttt gtctgctaca cgcaatgcta
     3841 agtgatggat atttccaaat ccttcgattg cttttggcaa gtctgtccga tgttcaataa
     3901 tgatgcttgc accatttccg ccttcaccga tttcaaataa gtagaaatct cctgaatggt
     3961 ctgtttcttt gaatcctaag attttttcaa gaatcaagcg gatatgctct aattgactga
     4021 cacggacaaa gacaggtcca agtcctgtga tcgcaaactc tgtcggtaca ttgctgtttt
     4081 tccaaggaat gccagctgct acaccttgat tgttttcgtc agagatcaat tgataatact
     4141 gattatcgaa atcttcaaat tcgatatatt ttttgccgaa acgttcattg attgctgaat
     4201 gagtaacgtc atatttttca aaacgttcca accaaaagtc taatgctgcg tcacttggca
     4261 cacggaaaga agcacgagag atcgtatctg ttcctttttt tccttttggc tgatttggaa
     4321 aatcaaagaa agtcatatct gttcctggcg caccagtatc gtctgtaaag taaagatggt
     4381 aagtttcgat gtcatcttgg ttaactgttt ttttgattaa gcgcattcct aaaatatctg
     4441 taaaaaagcg gtagattttt gtagcgctag atgtcattgc tgtgacgtgg tgaattcctc
     4501 tgatttgtgt atccatgata aatttcctcc tgtcaactta caaaaagtaa gtagttgatt
     4561 tagacatagt atagagggtg catctctatt ttgcaattaa tttgtcttct aaagtatcag
     4621 gaaggaatca agaatggaaa agaggaggga cggatcaata aaataaaaag acttacgact
     4681 agctgtatcg atttcaaaaa aactagcttt aaaaagctaa ctttagaatc attacgggtg
     4741 gtcataagtc tttttatgct gagttatttt ttcatttttg agctaatgct taggatagga
     4801 tagaatgttg atttcacgat ctcttcttgt tacttgaaac tgcttactac tgtcacgatc
     4861 tcttggtaaa cggctttttc acaacctctt catttaagtt gaacgcacat ccagcacaga
     4921 atagttccga acatcaaatc ggtttcggct ggaagaatat tcaatcggct gtccatcttt
     4981 gagatagacg acttgctgaa cttccagtac aggatcgttt tctgcacagt tcaaatattg
     5041 ctgatcatat tcatcacttt tatctgcaga aatagcacga taagcccctg caaaatggac
     5101 attcaattct tgatgaagat gatcatagat agaggctaac aaatgttctt tttttaaacc
     5161 cggcacaaga tggactggca taaaagtatg ctctaaaaca tatggctcgc catctaagat
     5221 ccttaaccgg tgaatattgt agacaggttg ctcggcatcg atcatcaatc gttcttggat
     5281 atattgatta gggaattcaa catcaaaatg aatgatttga ctcttcaaag aacgatgtct
     5341 gcgtttcatt tggagactca aaccttcata ctcgttagcg ggcgatgtgt ctttttgcat
     5401 aaatggatga ttcaagattt tcgttcctga tcctcgctgc gaatagacaa gtccttccat
     5461 tgctaagata ttgatggctt ttttgattgt catccggctc gcaccaaatt ctttgaccaa
     5521 gtccacttga ttaggcaaaa aactatcggg aggatactct tttgaacgga tacgttctct
     5581 taaaatattt gcgatttctt cgtattttgg cacgttattc acctcttcta gagtataacg
     5641 aaagattatg cttccgacaa ctcgaagtag catctggaac attgtttatt cgaaaaaaga
     5701 ggtcgtgaca agtttttgtc acaacccctt aatgatctat taagcaatat ctaaatctgg
     5761 ttgtttatct agtactttca ggaatgggta ccagataaag gcaacgatga caaagtcgat
     5821 gacttgcagc ataccaccaa gccatgaatt tgttgctaag acaccgcttg caatgattgg
     5881 cacagtccat ggaacagaga cgccagttgg tgcagggaca agtccagcag ccattaccaa
     5941 ataattgaac gtcgttacga ttagaggtgc cacaacccaa ggaataagga ttgttgcatt
     6001 caatacgatc ggtagaccga agatcgctgg ttcatttaca ttgaaaatac caggtgctaa
     6061 agctaatcgt ccaacatcac gatactgttt ctttttaaga acaaaggcca tcaataaaac
     6121 gacagctaaa gtcattccag agccaccaat accaacagtg aaggtttcca tgaatggttt
     6181 tgtgatgatg tgtggtaatt cttttccatg ttgataagct tctaagttat caagcatcag
     6241 tgtattccaa atcgggtcca ttacagagtt gacgatgatt tgtccgtgaa gtccgaagaa
     6301 ccataagaat tggacaaaga acaaagcaat caatgttgca ggtaaactac ttcccaaacc
     6361 agttaatggt ttttggatca cttcataaac gacgtcatgt aagttagcat tgaaaatacc
     6421 agtcatcaca gcattgatta ctaagaaaat agttagtgtc actacagcag ggatcaaagc
     6481 ggcaaatgat tttgttactg caggcggaac tccatcaggc attttgatct gccagccgcg
     6541 tttcgtgatt ctgcagtaga tttctccagc caagaaagca gcgatcattc caatgaacat
     6601 tccttttgcg ccaagacgat caagtgaaag cacaccagtc acatcttcac cgccagcggt
     6661 tttcattgca aaaggcgtaa gaatcaagaa gctggcaaaa gacacggcac caccgaaaat
     6721 accttcaacg tcataagatt ttgataaata atagccgata ccaaaagtaa caaaaaccga
     6781 catgatgctc attgttgcat tttgcccatt accgaataag ttcgataaag ttccctttgt
     6841 tgcgtcactg aaaaaaggca agttattgat aacgacaacg atcgaaccga acatcgttaa
     6901 agggaaggaa agcatgaaag catcccgcag cactgtaagg taacgatttt gccctaataa
     6961 attagctaag ggcatgattt tctcagaaag cttgtccata aaaccgttca tttttattca
     7021 cctcaaattt atttgcaatc gattacatac aaagtataac gccaaaaatt ttaaatgtct
     7081 agtcattttc ataaaaagta ttgatttatt ttttagggtt cgttatgatt acgatatcaa
     7141 gaaaaaagag aggaagtaaa aaaatgaaaa cctacacatt cccagaaaat ttttggtggg
     7201 gttctgctgc tagcggaccg caaacagaag gaagagtaag tgatgatggt aaaggagaaa
     7261 atatttggaa tcattggtat gaattagagc ctgaaaaatt cttcgatcaa gtaggacctg
     7321 aaaaaacttc gcaagtttac acaaaataca aagaagatgt tcagttaatg aaagaaactg
     7381 gccataacac atttcgtaca tctatccaat ggagtcgttt gattccagaa gggacaggtg
     7441 aaatcaatca aaaagctgtt tctttttatc gagattattt tactgaattg ctggataatg
     7501 gtatcgaacc ttttgtgaac ctttaccatt tcgatatgcc actaccactg caagaaaaag
     7561 gcgggtggtt atcaagagaa acggtcgacg cttatgtttc ttatgcagca gcgtgctttg
     7621 aactgtttgg agatctggtc aaaaaatggt ttactcataa tgaaccaatc gtacctgtag
     7681 aaggaggcta cttgtatcaa ttccactatc caaatcatat caatatgaaa gaagcggtcc
     7741 aagtaggcta tcatgagaat ctagcaagcg caaaggcaat caaagcctac catgaaggcg
     7801 gacatgatgg tgagatcgga atcatcttga atctgacacc aagctatccg agagatgaaa
     7861 cgaacccaga agatgtaaaa gcggcgaaga tcgctgacgc attcttcaac cgatcattcc
     7921 ttgaccctgc agtgaaagga gagtttccac aagagttgat cgaaattgtc aaagaattgg
     7981 ctataatgcc agagatccat gaaggagacc tagaactcat ccgtgaaaac acgattgacc
     8041 tgctaggaat caattactat cagcctcgtc gaatcaaagc aaaagaaact ccaatcaatc
     8101 acgaacatag ccccatgcca gatgattatt ttgataatta cgacatgccc aataaaaaaa
     8161 tgaatccata tcgtggatgg gaaatttacg aaaaaggaat ctatgatatt ttaacgaata
     8221 caagagaaaa ctatggtaat atcaaatgct atatttctga aaatgggatg ggggtcgaag
     8281 gagaagaacg atttgttaac agcgaaggaa ctattgaaga tgattaccgg attgagtttg
     8341 ttacggatca tttgaaatac gtccatcaag caatccaaga aggaaccaat tgtgtgggtt
     8401 accacatgtg gacatgtatg gacaactggt cttggacaaa tgcttataag aatcgctatg
     8461 gctttatcgc tgttgatttg gcacaagacg gtaaacggac gatcaaaaaa tcaggtcgct
     8521 ggtttaaaac ggtctctgaa aaaaacggat ttgatgcctg atattttttg ataaaacgtt
     8581 cacaaaatgt ttgttctaac actgtaatcg tgctacacta acagtgaagg aggcgtttac
     8641 aatggtagat acttatcgta atattttagt agcaattgat ggttctgaaa aggcagaaaa
     8701 agcatttctt gaggcaatca cgattgccaa acgaaatcag gcaacgttgc acatcctgta
     8761 tgtcaatgaa gtaacaggca attactttgg tgattttgca tttgttacaa caagccttca
     8821 ggaagaattg gatgaggttg cggaaaacca gatgaaagaa catcgaaact tggcaattga
     8881 aaaaggcttg acagatatcg agacatatgt tctttacggt tatccaaaga cactgattgc
     8941 taatttccat gaatcgaaag aaccaatcga tctgattgtc atgggggcga caggtctgaa
     9001 tgcggtagaa cgtgcactag taggttccac tacttcctat gtagtcaacc acgcatcatg
     9061 taacgtcatg gttgtaaaat aaataaatta agggaacaga aagcaaaaaa gcataacgct
     9121 tcttttgctt tctgttcctt taattttttg taaaaaatta ctttacctga tgaagtaatc
     9181 atgctattct aattacaacg acagatggag taatatggag gaaagaaaat gatttcaagt
     9241 gatggaatac gcggatacaa tgatgcgatt attttatcga tccttaacaa aaacgattct
     9301 tatggatatg aaatttctaa aacgattcgt gagaggacaa atgattccta tgtgatcaaa
     9361 gagacaacac tttactccgt ttttgcccgt ttagaaaaga atcaactgat tgtttcgtat
     9421 cctggagaag tgactcatgg aaaaaagcgg acatattata aaatcactga aatagggcgt
     9481 aatcttttag aagaaaaaaa gcaagagtgg ctgacgacga aagacgtagt cgatcaattt
     9541 ttggagggaa attaaatgga tacaatccgt aattatttag acagtctttt tatcggagta
     9601 cctcaaagta ccgagataga taagctgaaa actgatttgt tggcaaatat ggaagaccat
     9661 tatcatgaat tgatgggaga aggaaaaaat gaacaggagg ccatcggtac agtaatttcg
     9721 acatttggtt caatcgatga attattagaa gaactagatg tcgaaaaaaa acatcaagct
     9781 gacgagacgg aaacaaacac agcttctatt tatttaagcg aagcagaaaa ctactggaaa
     9841 gaatacagag cggcaagtct ccaagtggca tcaggtgttc tattcattag cttatctttt
     9901 gcaagttttc ttttcttttg ttcagcaggg tatgtgttca tgggtatttc ttgtctgatt
     9961 ttcggtatag cattagctgt cggttttttt attgcttctg gtatgaaaat cacacgttta
    10021 aatcactttt tgcaccatcg gaaaatacct gaaaaagtat tagcagaagc aaaagaaaaa
    10081 gaagaagagt atcagcgaag ttttggcttc tcactgattg caggaattgg attatgtatt
    10141 ttttcacttt tcccactttt ggcttcttta atgtggtata tggatggtag tatcggtgcc
    10201 tctatatttt ttgttacggt aggaacggga gtcttcttga tcatttatgg cagtctggtc
    10261 cgtcattctt atcgtcaatt cactcaaagt gcttattact ggtgaatgta ttcagatttg
    10321 taaaaatatt ttatttattg cttacccatg taaaatcgtt atagacagat ttcagttgac
    10381 ttctgcgcca ttgtacaggg ataaaaagac gcatactatc ttcattgaat ccgactaata
    10441 atttctttcc gtcagtaatg atgggacgtt gtaacaaagt aaaatgcgag acgatcagat
    10501 cgacaagctc tgaggtcgta cattggtcga tttgatcttt caaattttta taatcaatcg
    10561 agcgtttagc taatagatcg tcaaatccgt tttctgttaa tgacaataaa taaaatatct
    10621 ctttccttgt taaaggctgt ttaggtgtga aaattcgttc tctgtaaggc atttgatggt
    10681 ctttcagcca gtttgccatc agatggtaac tatgtgtgcg agaagaatag atcgtaagca
    10741 taaaaaaact ccttaaatag tccattaatt tgttagatct gttgagataa ataataggta
    10801 aggagctgtg cataagcatt gatcaaaaca acccaaacat cattagaaat aactttctaa
    10861 agtcttaaac ttgcgacgag aaacaactag actaatagaa aaaatgtctt agccatttgg
    10921 cttcctgttt gtttttttgc ttatgctgca gctcctcggg aaagggttct aacaatttga
    10981 agaaggttga tgattgatct agtttaaatt gtgtgaaatt tgaaatctgg tgtttttact
    11041 agatgaatca cctaacaact atgattataa cttatagaac ttctgttttg tacttggttt
    11101 tgttatagtt gttataaagt tttattttat tacagaacat ttttaattaa
//
LOCUS       DAZABD010000082        10844 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000082 DAZABD010000000
VERSION     DAZABD010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10844
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.083"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(135..1715)
                     /locus_tag="ACHALG_002421"
     CDS             complement(135..1715)
                     /locus_tag="ACHALG_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFN2587780.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(2116..3492)
                     /locus_tag="ACHALG_002422"
     CDS             complement(2116..3492)
                     /locus_tag="ACHALG_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFN2587781.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(3620..4708)
                     /locus_tag="ACHALG_002423"
     CDS             complement(3620..4708)
                     /locus_tag="ACHALG_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFN2587782.1"
                     /translation="MFWSLELLIIAALIMISSEINFIFEPIGTFFSTLFAPVLIAGFL
                     YYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQISSLASNMPDF
                     IKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSLGSIVGTIASA
                     TVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTLANYISGQAIE
                     CLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLVTVFNDPVKAL
                     LCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLGIFLGVPFYAI
                     CKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            4880..5314
                     /locus_tag="ACHALG_002424"
     CDS             4880..5314
                     /locus_tag="ACHALG_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN2587783.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(5392..5925)
                     /locus_tag="ACHALG_002425"
     CDS             complement(5392..5925)
                     /locus_tag="ACHALG_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HFN2587784.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(6019..7197)
                     /gene="mutY"
                     /locus_tag="ACHALG_002426"
     CDS             complement(6019..7197)
                     /gene="mutY"
                     /locus_tag="ACHALG_002426"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFN2587785.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(7190..7987)
                     /gene="recX"
                     /locus_tag="ACHALG_002427"
     CDS             complement(7190..7987)
                     /gene="recX"
                     /locus_tag="ACHALG_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFN2587786.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            8094..9476
                     /gene="rlmD"
                     /locus_tag="ACHALG_002428"
     CDS             8094..9476
                     /gene="rlmD"
                     /locus_tag="ACHALG_002428"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFN2587787.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(9650..10228)
                     /locus_tag="ACHALG_002429"
     CDS             complement(9650..10228)
                     /locus_tag="ACHALG_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HFN2587788.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            10550..>10844
                     /locus_tag="ACHALG_002430"
     CDS             10550..>10844
                     /locus_tag="ACHALG_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HFN2587789.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGEYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFF"
BASE COUNT         3230 a         2186 c         1874 g         3554 t
ORIGIN      
        1 acatttctat gattttaata tagtaaaaag agccgtcacg gaaaatccgt cacggctcta
       61 aacaaaatat gagaggctgt ttttgtttgg ctattcttat tacttgatgc agggcagctt
      121 gtgtcaagac ttctttacat caaacttttt aattttacat tgggatattt atccgcaaac
      181 cagcgttctg caaattggtt ctcgaataag aacaacggtt ggtcgaaacg gtcacgagcc
      241 aagatattgc gacttgaact catcttctca tccaaatctt ctggatcgat ccagcgagcg
      301 attttgtgtc ccattggaga catgattact tcggcgttat actcatttaa catacgatat
      361 tggaatactt cgaattgtag ctgaccaaca gctccaatga tatattcttc cgttagatat
      421 gttttataaa gctgaatcgc tccttcttga actaattgat agattccttt gtggaaggat
      481 ttttgtttca tgacattttt tgcagtcact ttcataaaca gctctggagt aaatgaaggg
      541 agttcttcat aagcaacttt tagttttcct tcgaacaatg tatccccaat ctgataattg
      601 cctgtatcat atacaccgat gatatcgcct gctactgctt tttcgacgtt ttctctcgaa
      661 tctgccataa attgtgtgac attgcttaat ttcagttttt tatttgtacg ttccaaccag
      721 acatccattc ctcgttcgaa tgttccagaa cagattcgga caaaagcgat tcggtcacga
      781 tgggctggat tcatatttgc ttggatcttg aatacgaaac ctgaaaattc ttcctcataa
      841 ggacttacca tctgatcatc ttgtgttttg tgtccataag gagctggtgc caattcaacg
      901 aaagtctcta aaaaagtctg tacaccgaag ttagtcaatg cagaaccaaa aaatactggt
      961 gtctgatcgc cacgagcaat tttttcttga ttaaatgcat ctccagcctc tttgattagt
     1021 tctacttctt ctaagacttg ttcatataag gaattttcag ttagaggatg attttcggga
     1081 agcagtccat cttcatccaa ttctactaat cgttcatcct gataattttc cggacgataa
     1141 aactctacac gattatggta gatatcataa agaccttcta gccctttacc cataccgatt
     1201 ggccaattca tcggataaga ttcgatatct aatagctctt ctaattcttc taacaattcc
     1261 aatggttcac gaccatcacg atctagtttg ttgataaaag taaatatcgg gatgccacgt
     1321 ttttttacaa cttggaataa tttctttgtc tgtgcttcga tcccttttgc actatcgatt
     1381 accatgaccg cactatccac ggccatcaaa gtacgataag tatcttcaga aaaatcttca
     1441 tgtcctggtg tatccaagat atttactcgt ttcccttgat aatcaaactg cataacagaa
     1501 ctagtaacag aaatcccacg ttgtttctcg atttccatcc agtctgattt ggcaaaatta
     1561 cctgtttttt tgccttttac ggtcccggct tgacggatag ccccaccaaa caatagtaat
     1621 tgttctgtaa tcgttgtttt cccagcatcc ggatgggaaa tgatcgcaaa agttctgcga
     1681 ttgtccactt gttgctttaa ttttggatta ttcataacct aactccattt cttccattca
     1741 attatttata ttttctctag gcacagattg tccgatctct taaagaaaga caatttcata
     1801 tagggctgtg acaaaagccg tgtagcagtc tctttttccg agtaaacggc gttctagaga
     1861 cagcctatca gcaataagcc cacccaagcc aaaaatacga aaagctgttt ttgcttggct
     1921 gctcttatta cttaatgcag gatagctttt tcacaacctt tgtcaactac tacaactgaa
     1981 tcagtatagc atggattcag cgaatgaaaa agccaccaaa ccataagagt caaatatttt
     2041 tttgtcagct gactaaaaga aaagacgtgt tgcttgttta ttcaagcaac acgtctttca
     2101 taaccaagtt tccaattatc ttcttggctc gtcatcttcg aattcttttc tgaagtatct
     2161 tcgttcttct tcaggttccg cttctgtttc ttccggatca tgaaaaatga cacggatttt
     2221 aaggattcga gaaccttcca tttcttcaga aaccagcgta atattatcga catcaaacga
     2281 taacttttcg ccttcatccg gaatcatgcc taatgctgta atcaaataac cagccatcgt
     2341 atctacatca ctcatatgca gatcactgtc aaaagcttca ttgaactcat caatcagcat
     2401 acgtccttgg atgatatatt cgtgttcatc gattttttca taaagattct caacttcatc
     2461 tgattcatca tcgatttctc cgacgatttc ttctaaaagg tcttctagtg ttgctaaccc
     2521 aacgacgccg ccatattcat ctaataaaat agccatctga ttctgcgtct ttttcatttc
     2581 atataacaga tcatcaataa agaccgtttc aggaacgaat aaaggctcct gcatgatttt
     2641 cttgatatct aaattatcaa agccgaattt atgggcagct tttagtaaat tctttgtatg
     2701 aaggattccg actactttat ctttgtcttc gttataaaca gggattcttg aatagttttc
     2761 ggataaaact tcattcacat tttcttccac agtgtcgttg atatcaatca taaatgcatc
     2821 cgttcgtggg accatgactt ctcgggcaac tttcgtatca agagaaaaaa ccccttggag
     2881 catttctaat tcttcattat tcaatacgcc ttcgttttca agcatataac gcatttcatc
     2941 tctggtcatt tttgaatctt catcatcaaa agtcatcggc gtgatacgcg ccaataaact
     3001 tgtggaagca gaaagcagcc agacgaaggg cttagctact acaccaatca agcgtatcag
     3061 ccctgaagtg aactttgcga cttcttctga tttatttaaa gcaatacgtt tagggtacaa
     3121 ttctccaaaa acaatcgata cataggtcaa aatagccagc acaatgatat ttgcaatatt
     3181 ttttgccgct gtgccgccac ctaataccgg agctaaccgt tccgataaag tattagctag
     3241 tgaagcacca gacaaaatgt tgaccaaagt gattcctact tggattgtcg ataggaaatt
     3301 atttggattc tgcaatattt ttaaaagctt ttgagatttc acatctcctt catcagcttt
     3361 ttgctcgatt cgattcttat tgatcgaaac aacagcaatt tcggaagctg ctaaaaaagc
     3421 attgatcaat gtcaaaacga tcaataataa aatctgcgct aacagcgact gactctcagg
     3481 gtcagcattc ataatcgcat tctctcctta taaaattaat ttttagttta cgatagaaaa
     3541 aaacgtggca ctatagccac gttaaaatgg tatcacaaag caattttctt tgcaataaaa
     3601 acaagtgtac taaaactacc tacgaggtcg tagcaatttt cttcttgttt tcattttgct
     3661 tgtcttcttt gacgattttg attatatagg aaatgatcgt cttgcagatt gcgtaaaaag
     3721 gaacacctaa gaagatacca agcaatccgg caatatttcc agctactaga agaacgataa
     3781 tgatcgttaa tggatggatt ttcagtgatt tcccaattac attaggataa atgatattcc
     3841 catccaactg ttggacgatt agcacaacca cacaacaaag taatgctttt accggatcat
     3901 tgaagactgt tactagaaat gcaggtgcta gtcctagata aggtcctaaa tatggaatta
     3961 ggtttgtcaa tcccgcaatg accccaaata agaaagcata atccacacca attaccatat
     4021 atcctaaaaa tgtgaaggta ccaacgaata aacattcgat tgcttgtccg ctaatgtaat
     4081 tagccaatgt tttattcagt tgccccaata actccacaat ttggtttctt cttttttctg
     4141 ggaaaaactg ttggatgttt ggcactagtt tttctccgtc tttcaacata tagaacaaga
     4201 tgaatggtgc tgtaatgatt actacggttg ccgaagcgat cgttccaacg atcgaaccta
     4261 aactattgga tactccacta aggaattgct gaatgattgt cccgtaagaa atgtctaatt
     4321 gctcaaaata tttattgata tctacttcct tgaacaaagg caattcggct acctctttta
     4381 accagctttc tacctgtttg atgaaatcag gcatattaga tgccaatgag gaaatttgct
     4441 gtactagact tgggatgaca ctaagtaaaa tccaaataat tgctgcaact aaaaggagca
     4501 gcacaattgc gacagcccat attcttttga tttttgcttt catcaacagc ttgacaatag
     4561 gattcaataa atagtaaaga aagcctgcaa tcagtacagg tgcaaataac gtggaaaaaa
     4621 atgtcccgat cggttcgaaa ataaagttta tttctgatga aatcatgatc aatgcagcta
     4681 tgatcaataa ttcaagcgac caaaacatta gtttagactg tctaagtttt tcaaacaata
     4741 gatttcctcc tttctttctt actagtatat attatattcg ttaaaaattc ttttattctt
     4801 tttttatttt tgaaaacttg ccatactttt tatttgtttt attttccagt atactatacg
     4861 aaaggaaggt ggctagacca tgaaagttgt acaaacaaaa gatacaatga gcaatattta
     4921 cttagatgct gtacggatca ggcatcaagt ttttgtagtc gaacagggtg tgcctttgtc
     4981 tcgagaaata gacaaagatg aagcccattg tatccacttc gtcctgtact cagataaaaa
     5041 agaaccgcaa ggaactgtac gtcttttgcc tttagaaaac ggaaaaatga aattacagcg
     5101 gatggcgatt ctttctgaat acagacacca agggttagga aaaatattga ttgaagaagc
     5161 agaaaacttt gctaagaatc aaggctataa tacgatttta ttaggggcac agtccacagc
     5221 tgaaactttt tacgaaaaac taggatatac agcttacggc gatccttttg aagatgctgg
     5281 catgcctcat atttatatga aaaagacttt ataaaaattt atttcgaaaa aagcaaaaaa
     5341 cagcaagctt ttgagaaaaa tctctcactg gcttactgtt cttttttgtg tttacttacg
     5401 cgacaattgc ttgtaacggt tataccaaat atcaatatag ccttccgaga acgggccatc
     5461 tccattattg atccagtcaa ccagaatctt cacattttct tttaggatga agtcaatatc
     5521 gttcgggtaa ttcatcattt tgctgtgcat ctcatattca tcgacatcca atagtcgttt
     5581 ctcgccgtca ggaaatacct tgatatccaa gtcatagtcg atatatttca aggcctcatc
     5641 gtccagcaaa aaaggtgaag ctagattaca ataataggaa accccctttt ctctgatcat
     5701 tgctattaca ttgaaccagt attttttatg aaaataaaca atcgctggtt cgcgcgttac
     5761 ccatcggcgt ccatcagatt cagtgactaa tgtgtgatca ttcacgccga tcatagagtg
     5821 ttcgcttgtt tttaatacca tggtatctcg ccacgttcga tgcaatttgc cgtcgtgctt
     5881 gtaactttgg atcgtcacaa actctccttc ttttggaact cccatagcaa cccttcctta
     5941 agcagcattt tgtgctttgt gtttgggtgt tcattacacc gacatcataa ttattatatc
     6001 aataaaaatg agctttgcct agaaaggatt cctattgttt cgatttttct ctaactgatc
     6061 taccagtttc atttggacct taggaaaaac gaccgaatca aatgcatcag gcttcaacca
     6121 cttcgaagtt ttgttctcgg taaattcttg ttctgctccc tcggttgccc gtccgtaaaa
     6181 aaggagaaca tgccatttca gatgactaaa aatatgagta acttctccta aatgtcgtgt
     6241 ctgccaaaca aagagttcct gcttttcaaa tgggagattt tgctcttctt ctgctactag
     6301 gtcatcaaac agatcgattt ctggctttgt ttcccattct tttttcaatc gctcatactc
     6361 ctcttgtgtc acttccatca ttggaaatgt ccacatattc gccagcaatt tttggctatc
     6421 tctttcttca aaataatacg ctccagaatg attttgaaga gctgcactga tataataaac
     6481 gtcttttggc tttgcttttt ttgtttttac aggaaaactc gtttggattc ctcgtttgtt
     6541 agctaaacaa aaggcttgga tcggacaagt ctcacacttt ggagaagttg gtgtacagat
     6601 agctgaacct agatccatca ttgcttggtt gaattcacct ggatgctttt catcaatgat
     6661 tttccgcatc gcttcatcaa agattttcct gctagaagct tttgcgatgt ctgcttcaat
     6721 gcaaaacaat ctgctgacta ctcgcatcac gttgccgtca actgcaggtt ctggaagtcc
     6781 aaatgcgata cttgcaatcg cgcctgtcgt atatggtcct attcccttca atgagctaat
     6841 ttcttcaggc gtttgcggca tttccccatc aaattctgac atgatctgtt tagcagcagc
     6901 ttggatgttc cgggctcttg agtaataacc aaggccctcc catgctttta aaagcttttc
     6961 ttctggagca tttgctagct cttcaattgt aggaaaccat tccatgaaac gataaaaata
     7021 atcgatcact gtgtctactc tggtttgctg aagcatgatc tcagagatcc agatacgata
     7081 aggatcacga ttatagcgcc aaggcaggtt tcttttttct tgttcatacc attgaatgaa
     7141 ctgatcttga aattcttttg tttctttatc tgtccatttt tcccattctt tattcacttt
     7201 cctcttcctt ttccgtaatg aatcgctgga tcatatccaa atcaaatcct ttttgatata
     7261 agctttgctt tacttttaga tttctttttt taggttcaaa ccttaaattt cttcgccata
     7321 gtttatctcc ttgtttgact aaataatcat attctatttc ttgatctgct tcttgcggaa
     7381 gatcagctat cgcttgttgg atcacttctt gagaaaagcc tttcgtcatc aatgtttgtt
     7441 ggatttttct caataattct ttttggcttt ttccgtgatt ttttccaaac gctttttcgg
     7501 ctgtctgatg tgcattttga acttggtctt caaatgtata ttggctgagc gcttgttcaa
     7561 tgatttcagg tttcaaacct ttttgctgca tctgctgcgc taatttcttt ggtcctttgt
     7621 cacttaagcg catattcgtg cgcacataac tttccccata gaccaaatca tcgacaaggt
     7681 ccaattcttt taagcgggct acgattttat ttcgatcttc ttgaaggatt tccttttctt
     7741 ttaagtatgt tcggatttct ttttccgatc gcaactgata acttaagtaa ttcattgcta
     7801 ggtgaaaacc gaaatcagct ccgctactct tcttgatctc ttggatagtt tcttcgtcta
     7861 attcccgtcc ttttaataat cgatagcgta ctaatacatc ttcagatact tgtaaagctt
     7921 ctccttcaga taaatctacg cgatagaaag ggcctttgcc tttactgatt ctcgtaacgg
     7981 ttaacataaa catcctcctc gaacatatat tctctatcat accacagaaa atccccgtaa
     8041 tcaggtatta tatgttagaa taaaccacag aaaaaagaaa aagggagata cttatggcag
     8101 aaacaattgt caaaactgga caaacggttc ctttgaaaat caagcgttta ggcataaatg
     8161 gtgaaggaat cggttatttc aaacggttga tcatatttgt tccatatgca ctaccaaaag
     8221 aagaagtact agtgaaaatc acgaaagcaa ctcctcgcta tgcagaagga gaattaataa
     8281 aagtcaaaaa aacaagcaaa gatcgcgtag tggcaccatg tcctgtttac tatgaatgcg
     8341 gtggctgtca attacaacac ttagcttatc aagctcaatt ggattttaaa aaggatctgc
     8401 ttttgcaagc attggaaaaa ttcaaaccag caggctttcg atcttatcaa cttttgccga
     8461 cgatcgggat ggatgagcct tggcattata gaaacaaagc acaatttcag ttacgaaaaa
     8521 ataagcagac tcaaaaaatt gaagctggtt tatacgaagc aaactctcat cagttagtcc
     8581 ctataacaga ctgcttggtt caagaacctc gaacacaaaa agtaatgaat actgttgttc
     8641 aactattgaa taaatttgat gcacccatct atgatgaacg gacaaacagt ggaatcttcc
     8701 gcacaatcat ggttagaatc ggtgttaaaa ctggggagtt acaagttgtt tttattactc
     8761 gcagtaaaaa attcccgcaa aaaaacgcaa tgatccgtga gatcaatgaa cgactgcccg
     8821 aagttgtttc aatcatgcaa aatgtccagg cacaaaaaac gtctctcgtg atgggggatg
     8881 aaacgatcca cttatgggga aaagaagcca ttgaagaaca aatcaatgaa gtgactttcg
     8941 atctttctcc tcgtgccttc tttcaactca atcctcagca gaccgaattg ctttatggcg
     9001 aaggaatcaa agcattagat gtccagccta atgaaacgat cgtggatgct tattgcggag
     9061 ttgggacgat cggcttaagt gtagcaaaac aagccaaaga agtacgggga atggataccg
     9121 ttcctcaagc aatcgctgat gcaaaaaaaa atgcggaaag attaggatat cacaacactc
     9181 gatatgaagt cgggacagca gagcaagttc tgcctaaatg gttgaatgaa ggttttcagc
     9241 ctgatggtat catcgtagat ccaccacgta cgggtttgga taacgcattg atccaatcat
     9301 tattgaaata ccctccaaaa aaacttgttt atatctcctg taatgtttct acacttgcca
     9361 gagatctggt taatttaagt aaaaagttca agattgaata tttacagtcc gtcgacatgt
     9421 ttcctcagac tgcacgttgt gaagttgtcg tgaagatgac gagactcgct gactaaatga
     9481 aagaggttgt gaaaaagtct gccctgtatc aagtaataag aacagccaaa caaaaatagc
     9541 tcttcatgtt ttatgaatac tgtacttgtg accgaagagt taacaggagg acactattta
     9601 tttaaaaaaa gggttgtgac atagtttttg tcacagccct ttttgattat tattggatat
     9661 tcacgtctat cacttgataa aatgcatttg tcgtatcgct gatattccat actgctaaaa
     9721 tcacattata tccttttcga tcctgcggaa gtgtgatcgt ttggttgact gtttttggtg
     9781 gaacagttgc tttgtcgtct atttttgtga tcaattcaaa atcactaaat ttcaaaggtt
     9841 gattaggatt ccaagttggt ttggtaatgt agtaatccca agtacttgtc cgatgttgtg
     9901 ctgttaaatt ccaagtaata tttaatgttc ctggattaac catgttttta tgccatctgt
     9961 tagatgtctg ttcatctaaa ggctcaaagc cgctgactcc tgcactagca atttttccat
    10021 caatgaatgt atttttggta gcttcgatac tttgcggttc ccattgtgct cttccgacat
    10081 tttgatttaa gtttcctgct gctgatgttc ctaggtatgc acggcttcct ggagaagtaa
    10141 tataaccatg cgcaaaacct ggaatgctaa aaccgaacaa ccctaaacta actaacccaa
    10201 taaacaataa cgtactaatc tttttcatgt agaaacctcc ttcatttgat aaccacattg
    10261 taaacgcttt attgtattaa cacaaacatt atgcttttat aattttatgt tgaagtaaaa
    10321 aaggtgaaaa aagtaagatg cagattataa cagaagtaga cgagaaaagt acaaatagaa
    10381 aacatgagaa tcaaatcata tgatcttttt caaaaacatc attcatataa acaaatgttc
    10441 aactttttaa taacaacaat tatcaatttt tttcttactt cacaaactaa atacaggtct
    10501 tgctgtgagc cttcatggta aactactatt atagaaagga gttagtaaaa tgaagatgat
    10561 aaaagatatt cgtttttggg tttgtgttat aggaattgtc atattaggct ttttatcagg
    10621 gttgcttagc ggaaatcccg gagaatatta ctattctttg caacttccac catttgcacc
    10681 acctagctgg atatttgggc cgatgtggac cttactgtat atattgatgg gtatttctct
    10741 ttatttgtta cttaatcaca acaacaaaaa acagcgaaac aatcttgtcg gtctttttgt
    10801 gattcagttc atttttaatt ttatatggtc tgctctattt ttta
//
LOCUS       DAZABD010000083        10614 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000083 DAZABD010000000
VERSION     DAZABD010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10614
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.084"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            287..2233
                     /locus_tag="ACHALG_002431"
     CDS             287..2233
                     /locus_tag="ACHALG_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFN2587790.1"
                     /translation="MIERHVNLLKLLSRQEDFKPASFFSDKLAVSTKTIYQDIELLED
                     YVVNYQVTIERLPSLGIKLIGDRKNVEHLISSVENYLEKDIYSPEKRRVEIIKEVLFG
                     DKPVSLTHLSQSFMVSKTSLYNDLRIINKIIGSGEVKLQSSAEGISFTGNEQAIQRAI
                     KQLVFYYANDMESKTFFQVLNMLFDSKVISVIHDLLFNDYSELTEKVSDYYVRSLIST
                     LVIQCNRLLKGHHLTVEEEFLFNSIRYMETYIVANGLIEELKAKLNLPFYDADKEYLC
                     RQLFAHRVTNNLKTSNEEYAMSVHKLIERMSEIEKIDLTQNDRLYQSLLYHVPAMVLR
                     LKKGIKIQNPLLENIKSQYSELFSIVWYAMSILEEEFDVTLTDDEVSLILIHFQIAIE
                     DQSKANNIIIICQYGISSAQLIYNKVRRFLPAKDNVEISTLDKFHLAEIGEVDLIISS
                     LSFDAGGIPLVRVSPLVSKTDYMNIMEAYTKYLIEEPSRFIQNELIPKAVKVPTISKF
                     VQSSLVKTGVQVESKEECLDLMISELEANSLVTEEFRASIFSREKMGNTNLESGVALP
                     HANPSMVIESNIFIVALKNAINWGDRKVKLVIMVSLAEEDVSEIRGVYEELYQFIGQK
                     EMVDRLLQVHDSEKLLRIFQQKGE"
     gene            2236..2691
                     /locus_tag="ACHALG_002432"
     CDS             2236..2691
                     /locus_tag="ACHALG_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFN2587791.1"
                     /translation="MYLDKELCLFQLEVKDQKELFQVMSEQLKNAGCVKDSFLEGITN
                     REQEFPTGLEVNQIGFAIPHTDSAHVNSSQICFASLKEPLVFSDMTDKSHEIPVRLVF
                     MLAMSQPHEQIDTLQNLVSLFQNEEKVNELLACTTKEAFIELVHSAGVY"
     gene            2705..4033
                     /locus_tag="ACHALG_002433"
     CDS             2705..4033
                     /locus_tag="ACHALG_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFN2587792.1"
                     /translation="MDIVLNAFEWFIGLGSNVFLPLIIFVIGILFGLKPGKAFVSGVT
                     VGIGSIGLGLVLSLLSDGLGTAIQQMGEQYGGGLNILDIGVGVGGPLAYSTSLGILMI
                     PISLILNFIFVVLGWTKTLNVDIWNFWFAIFLGMITQSVTGNFWFGLIGAIVAVILQW
                     FLADRTQKTVSEFFGYPGISITHMMGLSGVLFAKPVNWVFDRIPGFNKIDADAETLTK
                     RFGVLGDTVVIGLIIGIVVGIMAGYDVAGIGNLGIMTAAVMKIMPKMVAMFMEGLMPI
                     AEAAKEFTNKKLNGRQVNIGMDAALTVGHPTIMSTSLLLVPISLLLALILPGNKVLPF
                     GDLAFFAFAICLMIPYFKGNIVRSLVGCSLYVVTTLYMATWLAPIITDVFTLASFDVG
                     TAGLVTMLLCGLWPVGLFVLLASYFGVIGIGGLGVVLVGASIYMNKVKKA"
     gene            4067..4351
                     /locus_tag="ACHALG_002434"
     CDS             4067..4351
                     /locus_tag="ACHALG_002434"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFN2587793.1"
                     /translation="MKKLLVICGAGHATSTIAVAKLSKWLEEEGLTDQVKIYQSKIAD
                     ELNKIDEYDAVVSTTIVPDVIKAKVTNGLGLLTGIGTDAIYADVKERLGV"
     gene            4353..4868
                     /locus_tag="ACHALG_002435"
     CDS             4353..4868
                     /locus_tag="ACHALG_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HFN2587794.1"
                     /translation="MRFHHDTLTIETVSGRPSYHDIRKELLDTVDTAGIENGMLVISS
                     PHTTCSLFFEETMHDTNFFGDDYLHVDINNVMETIIPKMTSENQYNSPGPKHIDFGTR
                     LSDPNYPAEKWVMLNTDAHLRSSIYGTNSMTLIVQEGKLLLGEMGRVYFVDWDQLRER
                     TRTINLMIMGE"
     gene            4884..5546
                     /gene="rpe"
                     /locus_tag="ACHALG_002436"
     CDS             4884..5546
                     /gene="rpe"
                     /locus_tag="ACHALG_002436"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFN2587795.1"
                     /translation="MTIAPSLLNAPIAEVKELFEILKEKNVDYLHIDVMDGCFVPDMA
                     FGPSVIKDIRPLTDVVFDVHLMIDTPEKIVTRFVEAGADIVTFHIEATKHAMKVIQLV
                     KEAGAKVGITFNPATPVSFIEPVLSLVDQVLVMTINPGTENKHFIQETVVKIEQLDVI
                     RKQNDYTYDIEVDGKIDNQTIKVCSKAGADIFVSGGFIFNGKNTPQEQIDKLNQSLEE
                     VK"
     gene            5553..6125
                     /gene="hxlB"
                     /locus_tag="ACHALG_002437"
     CDS             5553..6125
                     /gene="hxlB"
                     /locus_tag="ACHALG_002437"
                     /EC_number="5.3.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0043800 - hexulose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-3-hexuloisomerase"
                     /protein_id="HFN2587796.1"
                     /translation="MTNKDQNKNYLEAIILELQNNSKRINSVELEQFSEKIVQAKHIF
                     LAGAGRSGIAMQAFTNRLMHLGFSVSLVGEISSPHSQPGDLLIICSGSGETGSLKNLA
                     AKANQSGVSIALVTMKKDSSIGQLADSVLVLPGITKDENQREEDTFSQPMGSSFEQLA
                     FLTFDSLVLSLMDKTGESSETMFARHADFE"
     gene            complement(6312..7832)
                     /locus_tag="ACHALG_002438"
     CDS             complement(6312..7832)
                     /locus_tag="ACHALG_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFN2587797.1"
                     /translation="MTKMESELLVQIKQLTQLLEIQQQENTLLREQIAEMNRRLFGRK
                     KETPPVDGQIDLLDDSTFNEPEHTGQESQEPITVSSFKRRKRKGLKALSLEGLPEVPI
                     HYELKGADCLCEDCGHTLHDIGATRLREEPLYVPAHIEKQVIYQHAYHCRECEALGET
                     KIKKAPVPKPLINNSLGSSSIVTQSIWEKFVKKVPAYRQEKEWHALGFPLDRKKITNW
                     HIKVSEMVLEPIYNCLHQELSNQEVLHADETSYRVLNTHKEKTFYWLYASGKSEEKQV
                     VLYEHANSRSVDEPKSFLESFSGFLHTDGYAAYGKLPVNNVYCWAHVRRKFFEALGKQ
                     PSPKSHAKIGLDFCDKMFRIERTFNKLSAEERFVARKETLQPVMTEFFHWCESFAYLP
                     KSQLGKAVQYAVNHKDGLQIVLLDGRLELSNNRAERAIKELVIGRKNWLFSKSLKGAR
                     SNGIILSIIQTAVANGLNIRKYLNHLFTEIPNLSSMTPEALRAYLPWNQQIQEICK"
     gene            complement(8075..8260)
                     /gene="tnpB"
                     /locus_tag="ACHALG_002439"
     CDS             complement(8075..8260)
                     /gene="tnpB"
                     /locus_tag="ACHALG_002439"
                     /inference="COORDINATES: protein motif:HMM:NF017528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFN2587798.1"
                     /translation="MPMLIDATALKHIFIVCGKTDLRKGIDGLAAFVIEEYDLDVYDQ
                     ALFLFCGTKKDRFKALY"
     gene            complement(8244..8426)
                     /locus_tag="ACHALG_002440"
     CDS             complement(8244..8426)
                     /locus_tag="ACHALG_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587799.1"
                     /translation="MNKKDIVPVQFSSAAQKSQIDTKPKLACRIKSEDKEIFIYNGMD
                     KYLLYTLLKELNANAH"
     gene            8667..8885
                     /locus_tag="ACHALG_002441"
                     /pseudo
     CDS             8667..8885
                     /locus_tag="ACHALG_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            9380..9979
                     /locus_tag="ACHALG_002442"
     CDS             9380..9979
                     /locus_tag="ACHALG_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN2587800.1"
                     /translation="MKTVEPIRDKKKIDAMKAILASGKYGQRNLVLFSIGINTAYRIS
                     DLRQLKLADVLEISRGRVVVKDRLAMKEQKTAKHNSVYISNKLRKTILEYVQQEFPDQ
                     LLQQDFDGYLFPSRKGTNEPLNRQSLWRIIHDAGTAIGLKDIGPHSMRKTFGYFLYKQ
                     GTKTEIIQSLLNHSSQRETLRYIGITQEDKDTAVKSLDL"
     gene            10043..>10614
                     /locus_tag="ACHALG_002443"
     CDS             10043..>10614
                     /locus_tag="ACHALG_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbiH family protein"
                     /protein_id="HFN2587801.1"
                     /translation="MSTLYIIGNGFDLSHGLKTRYTDFSKWVEKSHSEIFDKIDTMIN
                     ENNEVRSTADDKIGWFNFESSLHELIQSPVFEDYVEESKDYLIGYGHEDWSDSAHHDF
                     QNMVYDYVVSLEELQQLFKEWIRKEIQPTIEHKIPKYQFSSDVLFFTFNYTDVLEILY
                     KQPKENIYHIHGSYDDIIIGHSENFDNFIG"
BASE COUNT         3510 a         1670 c         1982 g         3452 t
ORIGIN      
        1 ataataaaaa aatacgaatt ttagtgacgc cattatattg tttttatttt tcaattgtaa
       61 tatttcatat tacttccata ataaggagta ttttctattg gaatatctta gctcttttaa
      121 ggatttctcc ttttttgttt ctcatttttt aagattattt ggatttcctt tacaacaaac
      181 ttccacaagt attattgaca tagatgtaat tgagggaagg gggttccagt gctagaataa
      241 ataatgaaaa gaggtaacaa atgctagcgt taagaggtga acaagtttga ttgaaagaca
      301 tgtaaattta ttaaaattat taagtcgaca agaagacttt aaacccgctt ctttttttag
      361 tgataaattg gcagtgtcga ccaagaccat ttatcaagat attgaattgt tggaagatta
      421 tgtagttaat taccaagtaa caattgagcg attaccttct ttgggtatca aactcatagg
      481 tgataggaaa aacgttgaac atttgatttc atcggtagaa aattatcttg aaaaagatat
      541 ttattctcca gaaaaaagac gtgtcgagat cattaaagaa gttttgtttg gtgataagcc
      601 tgtttcttta actcatttgt ctcaaagttt tatggttagt aaaacatcgt tatacaatga
      661 cctgagaatt atcaataaaa ttattggcag tggagaagtt aagttgcagt catctgcaga
      721 aggaattagt tttactggta atgaacaagc aattcaacga gcaatcaagc aacttgtatt
      781 ctattatgcg aatgacatgg aatcaaaaac attttttcaa gttttaaata tgttgtttga
      841 ttcaaaagtt attagtgtca ttcatgattt gctattcaat gattacagtg aactgactga
      901 aaaggtttct gattactatg ttcggtcatt gatttctact ttagttatcc aatgtaaccg
      961 tttactgaaa ggtcatcatt taactgttga agaagaattc ctcttcaata gcattcgcta
     1021 tatggaaacg tacattgtgg caaatggttt aattgaagaa ttgaaggcaa aattgaatct
     1081 tccattttat gacgcagata aagaatatct atgtcgtcaa ctatttgcac atcgtgtgac
     1141 aaataattta aaaaccagca atgaagagta cgctatgagc gttcataaat tgattgaaag
     1201 aatgagtgag attgaaaaaa ttgatttgac tcaaaatgat cgattatatc aatccttatt
     1261 gtatcatgtg ccagcaatgg tccttcgttt aaaaaaaggt attaaaattc aaaatccgtt
     1321 attagaaaat atcaaatctc aatattcaga actctttagc atagtatggt acgccatgtc
     1381 catccttgaa gaagaatttg atgtgacttt aacggacgat gaagtttcct taattttgat
     1441 tcattttcag attgctattg aagatcagtc caaagcaaat aatatcatca ttatttgcca
     1501 atacggtatt tcttctgcgc aattaattta caataaagtt cgacggtttt taccagcaaa
     1561 agataatgta gaaatatcaa ctttagataa gtttcattta gctgaaattg gggaagttga
     1621 tttgattatt tcctctcttt cctttgatgc aggtggtata cccttagtaa gggtatctcc
     1681 attggtaagt aaaacagatt atatgaatat tatggaagct tatactaaat atttaataga
     1741 agaaccttcg cgatttattc aaaatgaact gattcctaaa gcggttaaag ttcccaccat
     1801 atctaaattt gttcaatcat ctttagtaaa aacaggagta caggtggaaa gcaaagaaga
     1861 atgtttggat ttgatgattt ccgaactaga agccaattca ttagttactg aagagtttcg
     1921 tgcttctatc ttctctagag agaaaatggg gaatactaat ttagaaagtg gtgttgcttt
     1981 accgcatgcg aatccatcaa tggtcattga atccaacatt tttattgtag cattaaaaaa
     2041 tgcgattaat tggggcgatc gaaaagtcaa attagtcatt atggttagtt tagctgaaga
     2101 agatgtatct gagattagag gagtatatga agaactttac caatttattg ggcaaaaaga
     2161 aatggtcgat cgcttgttac aagttcatga tagcgaaaaa ttactaagaa tttttcaaca
     2221 aaaaggagaa taaaaatgta tctagataaa gaattatgtt tatttcaatt agaagtgaaa
     2281 gatcaaaaag aattgtttca agtaatgtct gaacaattaa aaaacgctgg ttgcgtgaaa
     2341 gatagtttct tagaaggtat caccaaccgt gaacaagaat ttccaactgg attagaagta
     2401 aatcaaattg ggtttgctat cccacataca gattccgcac atgtaaatag ctctcaaatc
     2461 tgttttgcct cattaaagga accactggta ttttcagata tgacagataa atctcatgaa
     2521 atacctgtac gtttagtctt tatgttggca atgtcacaac ctcatgaaca aattgataca
     2581 ctacaaaact tagttagcct atttcaaaat gaagaaaaag taaatgaatt acttgcatgt
     2641 accacgaaag aagcatttat tgagttagta cattctgcag gagtttatta ggaggaatag
     2701 agagatggat attgtattaa atgcctttga atggtttatt ggattagggt caaacgtctt
     2761 tttaccactg attatttttg taatcgggat attatttgga ttaaaacctg gtaaagcatt
     2821 tgtttccggc gttactgttg gtatcggttc tattggttta ggattagttt taagtttatt
     2881 atcagatggt ttaggaacag caattcaaca aatgggggaa caatacggtg gtggcctgaa
     2941 tatcctagat attggcgtag gtgttggtgg acctttggct tactctacat cattgggaat
     3001 tttaatgatt ccaatttcat taattcttaa cttcatattt gtggttttgg gttggacaaa
     3061 aacattgaat gttgatattt ggaacttttg gttcgcaatt ttcctaggta tgattactca
     3121 atctgttacg ggaaactttt ggtttggctt aattggcgcg attgtcgcag taattttaca
     3181 atggttctta gcagatcgta cacaaaaaac tgttagcgaa tttttcggct atccaggaat
     3241 ctctatcaca catatgatgg gattatcagg agtactattt gccaaaccag taaattgggt
     3301 ttttgatcgt attcctggat ttaataaaat agatgcagat gcagaaacat taacaaaacg
     3361 tttcggtgtt ttaggtgaca cagttgttat tggcctaatt atcggtattg tagtgggtat
     3421 tatggcaggc tatgacgtag caggaattgg aaacttagga attatgactg cggcagtgat
     3481 gaagattatg cctaaaatgg tagcaatgtt tatggaaggt ttaatgccaa ttgcagaagc
     3541 ggcgaaagaa tttacgaata aaaagttaaa tggtcgccaa gtaaacattg gaatggatgc
     3601 agcgttaact gtgggtcatc caactattat gtcaacttct ctattgcttg taccaatctc
     3661 attattgcta gcactaattc ttccaggaaa taaagtatta ccttttggag atttagcatt
     3721 ctttgctttc gccatctgtt taatgattcc ttattttaaa ggaaatattg tgagatcatt
     3781 agtaggatgt tcgttgtatg ttgttacaac attatatatg gcaacatggt tggcaccaat
     3841 tatcactgat gtgtttacat tagcaagttt cgatgtgggg acagcaggat tagtaactat
     3901 gttgttatgt ggattatggc cagtagggtt gtttgtttta ttagcatctt actttggtgt
     3961 aattggaata ggtggattag gagttgtttt agtaggtgct tctatctaca tgaacaaagt
     4021 gaagaaagct taatcataaa attaaaataa gaaagaagag atcaatatga aaaaattatt
     4081 agttatttgt ggagcaggtc atgctacttc tacaattgca gtagcaaaat tatcaaaatg
     4141 gttggaagaa gaaggattaa cagatcaagt gaaaatttat caatctaaaa ttgctgatga
     4201 gctaaataaa attgatgaat atgatgctgt agtaagtaca acaattgtcc ctgatgtaat
     4261 taaagctaaa gtaacgaatg gcttgggctt attaacaggt attggtacag atgccattta
     4321 tgcagatgta aaagaaagat taggtgttta atatgcgttt tcatcatgac actttgacta
     4381 ttgaaacagt ttcagggcgt ccttcgtatc atgatattcg aaaagaactt ttagatacag
     4441 ttgatacagc aggtattgag aatggaatgt tagtaatttc gagcccgcat acaacttgct
     4501 cactcttttt tgaagaaacg atgcatgaca caaacttttt tggtgatgat tatttacatg
     4561 tagacatcaa caatgtaatg gaaacgatta ttccaaaaat gacgagtgaa aatcaataca
     4621 acagtccggg gccaaaacac attgattttg gaacaagatt atctgatccc aattaccctg
     4681 cagaaaaatg ggtcatgcta aataccgatg cacatttacg ttcatctatt tatggaacca
     4741 actcaatgac tttaattgtt caagaaggta aacttttatt aggagaaatg ggccgtgtct
     4801 attttgtcga ttgggatcaa ctaagagagc gaactagaac aatcaatctc atgattatgg
     4861 gagaataaac gggggaatag agaatgacaa ttgctccatc attgttaaat gcacctatcg
     4921 cagaagttaa agagttattt gaaatcttaa aagaaaagaa tgttgattat ttgcatattg
     4981 atgtaatgga tggctgtttt gtaccagata tggcttttgg accaagtgtt attaaagata
     5041 ttcgtccact aacagacgtt gtgtttgatg ttcatttgat gattgataca ccagaaaaaa
     5101 ttgtcacaag atttgtagaa gcaggagcag atattgttac cttccacatt gaagctacca
     5161 aacacgcaat gaaggtcatt cagttagtca aggaagcagg agcaaaagta gggattactt
     5221 ttaatcctgc aacaccagtc agttttattg aaccagtatt atctttagtc gatcaagtat
     5281 tagtaatgac aatcaatcca ggaacagaga ataagcattt tattcaagag acagtagtta
     5341 aaatcgaaca gttagatgta atacgcaaac aaaatgatta tacgtatgat attgaagtag
     5401 atggcaagat tgataatcaa acgattaaag tttgttctaa agcaggggcg gatatttttg
     5461 tatcaggcgg tttcatcttc aatgggaaaa ataccccgca agaacaaatt gataaactaa
     5521 atcaatcatt agaggaagtg aagtaaaaga tcatgaccaa taaagaccaa aataaaaact
     5581 atttagaagc gattatctta gaattacaaa ataactcaaa acgtattaat tctgtagagt
     5641 tagaacaatt tagtgagaaa attgttcaag ctaaacacat ctttctagct ggtgcaggac
     5701 gctctggaat tgccatgcaa gcatttacga atcgcttaat gcatttaggt ttttcagtca
     5761 gccttgtcgg tgaaattagt tcaccacatt cgcaacctgg agatttatta attatctgtt
     5821 ctggctcagg tgaaacaggt agtttaaaaa atttagcggc caaagcaaat caaagtggtg
     5881 taagcattgc tttagttacc atgaaaaaag actcgtcgat tggtcaatta gcagattctg
     5941 ttcttgtatt accaggtatt accaaagatg aaaatcaacg agaagaagat actttttcac
     6001 aacctatggg ttcaagtttt gaacaattag catttctcac ctttgatagt ttagtgttat
     6061 ctttaatgga taaaacaggt gaaagctcag aaacgatgtt tgctcgacat gcagattttg
     6121 agtagtagat tttaaagata aaaaaccatt ctctaaatgc taattattca gcaaatatga
     6181 gaatggtttt tttatttgat tagacataac ttaataattc agtagttttg agcgtaagcg
     6241 tgtcatagaa tggtacctaa acacaaatag cccgatttga tacactcaaa tcaggttatt
     6301 tgtggcttat attatttaca gatttcttgt atctgctgat tccatggcaa ataagcgcgt
     6361 agcgcttcag gtgtcatcga agacagattg ggaatctctg taaaaagatg atttaaatat
     6421 ttgcggatgt ttaacccatt cgcaacagct gtctggataa tactcaatat aattccattt
     6481 gatctagcgc cttttaagct tttagaaaat agccagtttt ttcgaccgat cactaactct
     6541 ttgatggcac gctctgctcg gttatttgaa agttccaagc gtccatcaag caacacaatc
     6601 tgaagtccgt ctttatgatt gactgcgtat tgaaccgcct ttccaagctg cgattttggc
     6661 aaataagcaa aactctcaca ccaatgaaaa aattcagtca tcacaggctg caacgtttcc
     6721 ttcctcgcta caaacctttc ttctgcgcta agcttattaa aggtccgttc gattcgaaac
     6781 atcttgtcac agaagtctaa tcctattttg gcatgacttt ttggggacgg ttgtttaccc
     6841 aatgcttcaa aaaactttct gcgaacatgc gcccaacaat aaacattgtt taccggcagc
     6901 tttccatagg ccgcgtaacc atcagtatgc aagaaaccgc taaatgattc taaaaaactt
     6961 tttggctcat caacggaacg cgaatttgcg tgctcgtaga gcacaacttg tttttcttcg
     7021 cttttacctg aagcatataa ccagtaaaag gtcttttctt tatgagtatt caatacccga
     7081 tagcttgtct catccgcgtg taagacctcc tgattgctta attcttgatg aaggcaattg
     7141 tagattggct ctaaaaccat ttcacttacc ttgatatgcc aattagtgat ttttttacga
     7201 tcaagaggga aaccaagtgc atgccattct ttttcttgtc gatatgccgg caccttttta
     7261 acaaattttt cccaaatact ctgtgtcaca attgaagagg aacctagact attattgatc
     7321 aacggtttag gaacaggcgc ttttttgatt tttgtttctc ctaatgcttc acattctctg
     7381 caatgatagg catgttggta gatgacttgt ttttctatat gggcaggaac atacaatggc
     7441 tcttctctaa gccttgtcgc accgatatca tgtaaagtat gaccacagtc ttcacacaaa
     7501 caatcggcac cttttaattc ataatgaata ggaacttcag gcagaccttc taatgaaagt
     7561 gctttgagtc ccttgcgttt tctgcgttta aaagaagata ccgtaatggg ttcctggctt
     7621 tcttgtccag tgtgctctgg ttcattaaaa gttgagtcgt ctaataaatc gatttgccca
     7681 tctacgggtg gtgtttcttt ttttcgtcca aacaagcgtc gattcatttc agcaatttgt
     7741 tctctaagaa gagtattttc ctgttgctgg atttcaagca actgtgttaa ttgcttgatt
     7801 tgtacaagaa gttcagattc catttttgtc atttttacac caccattcta gcgattttct
     7861 tacatctatt ataccatttt atgacaacga aaaacagaca aaatccttga tttaacaagg
     7921 ttttcgtcta atatatcgca ccttttttcg tttcttttag cgtacatttt tcttcgattg
     7981 aaaggccagt cagtaagact tcttttctgg aacgcggcca ttgaaggtac ccactttcaa
     8041 atcttttgta cattaataca aatccatttt gatcctagta aagagccttg aagcgatctt
     8101 tcttcgtacc gcaaaataag aaaagcgctt gatcgtaaac atcaaggtca tattcttcta
     8161 taacgaaagc tgctagtcca tcaattcctt ttcgaagatc tgtttttcca caaacgataa
     8221 aaatatgttt caatgcagtt gcatcaatga gcattggcat tcaactcttt taacaaggta
     8281 tacaaaagat atttatccat tccattataa ataaatattt ccttatcttc cgatttgatt
     8341 ctgcaagcta gttttggctt ggtgtcaatt tgcgattttt gagccgcact tgaaaattga
     8401 actggtacaa tatctttttt attcattcgt ttttctcctt ctatatttga tagtttaatt
     8461 atagaagaaa gataatcggc gtacgagata ctatcctatg acacgcttac atttaattta
     8521 atattttgcc tcaaattagc cacacttttt atagaactaa gaggaaaaat gaagttttct
     8581 tcctattaat atagttgttt gaaatcttaa atatagcgtt ttacgatgta atgggaagta
     8641 tggtgaataa atcaactatg ttcgtacgaa gcatttactc aactaattta atcgaatctt
     8701 tcaataaaca aatcaagaaa tacagccacc gcaaggaaca gttccaaaat gaagagtcga
     8761 tggaacgttt cttagtctcg tcttttgata cttacaacca aaaattccta ggtcgcagtc
     8821 ataaaggttt ccaacaggcc gaaggcgagc tcgaacaaat gctgagtcaa ccgatggaga
     8881 attagaaaca atctacagta gggaagaacc attgacacaa aattattgac gctcccaact
     8941 aaaagtgtca aaataatgat gaaatcattt agttcttata aattagaaaa gagaaaataa
     9001 atattccata tctactttat tggatccgta acatgactaa attctgaata ctttttaaag
     9061 cttatcattt ggtaaaactg aatataagtt aattatatag actgaaaatt ttgaattttc
     9121 agaatagaat aacttaaaag tactgacaaa attttttaaa ggggcaatat taatgttctg
     9181 agataatcaa atcctaccga aaatatcaaa aatccaatgt gacacaatca gattgtgtct
     9241 cattcgaggg aatcacacaa tcccacgtgg ctctaggcag aattgaattt ttgcttgggg
     9301 ctttttcctt atattgattt tgaatgagac ttattatatg ttataatgca attaagtatc
     9361 attggagagg gcgaagaaaa tgaagacggt agaacctatt cgagacaaaa aaaagatcga
     9421 tgcgatgaag gcaatcttgg cttcgggaaa atatggccaa cgaaatttgg ttcttttttc
     9481 gattgggatc aacaccgcct atcgcatttc tgatttgcgt caattgaagc tggccgacgt
     9541 gctggaaatt tcacgcggtc gcgtggtggt caaagatcga ttagcgatga aggaacaaaa
     9601 aacagctaaa cataattcgg tctatatttc caacaaattg cgaaaaacga ttttggagta
     9661 cgttcaacag gaatttcctg atcaactcct tcagcaagat tttgacggtt atttgttccc
     9721 tagtcgaaaa gggacgaatg aaccgctcaa ccgccaaagt ctgtggcgaa tcattcatga
     9781 tgctggcacg gcaatcggat tgaaagacat tggcccacat tccatgcgaa aaacgttcgg
     9841 ttactttctc tataaacagg ggaccaaaac agaaatcatt cagtccttgt tgaaccattc
     9901 ttctcagcgc gaaacattgc ggtatattgg tattacacaa gaagacaaag atacagctgt
     9961 aaaaagctta gatttataat ataaaagtta agtttaaaca tggatagttt taaattagta
    10021 gattgagtgt tgggaggaaa gaatgagtac attatatatt ataggaaacg gttttgattt
    10081 aagtcatggt ttaaaaacta gatacactga tttttcaaaa tgggttgaaa aaagtcatag
    10141 tgaaatattc gataaaatag acacaatgat aaatgaaaat aatgaggtga gaagtacagc
    10201 tgatgataag ataggttggt ttaattttga aagtagtttg catgagttaa ttcaaagtcc
    10261 agtctttgaa gattatgtag aagagtctaa ggattatctc ataggttatg gacatgaaga
    10321 ttggtcagat tctgcccatc atgattttca aaatatggtt tatgattatg ttgtttctct
    10381 agaagaatta caacaacttt tcaaggaatg gatacgcaaa gaaatacagc cgacaataga
    10441 acataaaatt ccaaaatacc aattttcgtc cgatgtacta ttctttacat tcaattatac
    10501 agatgttttg gaaattctat acaagcaacc gaaagaaaac atatatcata ttcatggcag
    10561 ttatgacgat ataataatcg gccactccga gaattttgac aactttatag gaga
//
LOCUS       DAZABD010000084        10586 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000084 DAZABD010000000
VERSION     DAZABD010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10586
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.085"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            23..646
                     /locus_tag="ACHALG_002444"
     CDS             23..646
                     /locus_tag="ACHALG_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587802.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1096..2145
                     /gene="trpX"
                     /locus_tag="ACHALG_002445"
     CDS             1096..2145
                     /gene="trpX"
                     /locus_tag="ACHALG_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan ABC transporter substrate-binding
                     protein"
                     /protein_id="HFN2587803.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2142..3023
                     /locus_tag="ACHALG_002446"
     CDS             2142..3023
                     /locus_tag="ACHALG_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN2587804.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3026..3796
                     /locus_tag="ACHALG_002447"
     CDS             3026..3796
                     /locus_tag="ACHALG_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN2587805.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4075..4929
                     /gene="aroE"
                     /locus_tag="ACHALG_002448"
     CDS             4075..4929
                     /gene="aroE"
                     /locus_tag="ACHALG_002448"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFN2587806.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5107..6270
                     /locus_tag="ACHALG_002449"
     CDS             5107..6270
                     /locus_tag="ACHALG_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFN2587807.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGGQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6387..6797
                     /locus_tag="ACHALG_002450"
     CDS             6387..6797
                     /locus_tag="ACHALG_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFN2587808.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6971..9199
                     /gene="topB"
                     /locus_tag="ACHALG_002451"
     CDS             6971..9199
                     /gene="topB"
                     /locus_tag="ACHALG_002451"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFN2587809.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9370..9654
                     /locus_tag="ACHALG_002452"
     CDS             9370..9654
                     /locus_tag="ACHALG_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587810.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9814..10050
                     /locus_tag="ACHALG_002453"
     CDS             9814..10050
                     /locus_tag="ACHALG_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFN2587811.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10047..10446
                     /locus_tag="ACHALG_002454"
                     /pseudo
     CDS             10047..10446
                     /locus_tag="ACHALG_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
BASE COUNT         3694 a         1770 c         2237 g         2885 t
ORIGIN      
        1 aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat cgagagtaca
       61 agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt attttcgacg
      121 tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt tattgccgga
      181 aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa ccagcaaatt
      241 gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg cgatatcggt
      301 gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt ttaatgatcc
      361 tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt atgatgtaca
      421 tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt tctttgatgg
      481 aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt ttgataagga
      541 gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag aagaagaatt
      601 ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca ttggcgcgat
      661 gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg tttgatcttc
      721 aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt cgattagact
      781 taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt atttcagcag
      841 aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg agactttccc
      901 atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga catgaacatt
      961 gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga ccgctttggc
     1021 cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa agattgactt
     1081 tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat tattttagtt
     1141 tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag tacttcgacg
     1201 aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca tccggcatta
     1261 gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa agaaggagaa
     1321 aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc cacaatgagt
     1381 caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc tgccgctcaa
     1441 tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac ggatcctgtg
     1501 ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg tgtttctgat
     1561 cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa tgcaaaaaaa
     1621 gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc acaagcagaa
     1681 gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc cactaatgag
     1741 attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt tccattagat
     1801 aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc aaatatcccc
     1861 gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat cgggatcaat
     1921 caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag tggaaaagaa
     1981 aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat caatcaatct
     2041 caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc caaaatcatt
     2101 acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta tcagctattt
     2161 cacaaggcat gctttgggca atattaggat tagggatttt catgacttat cggatcttag
     2221 actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc tgtgtgacag
     2281 ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt gctggcatga
     2341 tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt attttagcag
     2401 ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga acaccaaatc
     2461 tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg ttacctgatt
     2521 actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct gtactgctat
     2581 tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac gaaacgatgg
     2641 ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact ttgtcgaacg
     2701 gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca gatgtaaata
     2761 aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa gtgatcttcc
     2821 aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc atttatcagc
     2881 tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag ttgttctccg
     2941 ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg aacttaaaaa
     3001 cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa atggcagaaa
     3061 agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta accttacaat
     3121 cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa gcacactgtt
     3181 caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta aagatcagct
     3241 gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt tccaagaccc
     3301 caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg cacaaaaaag
     3361 aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag attttttcag
     3421 actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag cgggtgaatt
     3481 atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag ctcctgagtt
     3541 actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag cattaatgcg
     3601 attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca cgcatcgaat
     3661 ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga aaatcgttca
     3721 agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc agttctttga
     3781 agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact tgaaaactca
     3841 ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa catatttggg
     3901 tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt ctttgctctg
     3961 acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc gtactttttc
     4021 gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa taagatgatt
     4081 tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag tttatcccca
     4141 ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt agctttccaa
     4201 gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga tatgttaggt
     4261 gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga ggtaagccag
     4321 gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg tttgattggc
     4381 tataatactg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat ttcgatcaaa
     4441 aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac tgttcaagca
     4501 gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac attcgctcaa
     4561 gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa agttcgtgac
     4621 tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct agtcaatgca
     4681 acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat aagcatgatg
     4741 catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg tttattaaaa
     4801 gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat ctaccaagga
     4861 gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat tgaaaagctg
     4921 tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg gtctcaagaa
     4981 aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat tatcaaaaaa
     5041 tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa acgaggagga
     5101 accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa acctctgaag
     5161 cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat ggaaaatgag
     5221 gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt gaaccaatac
     5281 gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa taaaaaattt
     5341 ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc gtggaaaaaa
     5401 atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt ttttattggt
     5461 aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac ctttcatggg
     5521 caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac acgcggagaa
     5581 gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc ccataagccg
     5641 aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg acaaaataag
     5701 atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt agtagaaaat
     5761 tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga aaaagtgatc
     5821 gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa tctggatggt
     5881 gtagatatta aaaaaggggg ccagaagatg gatggacatg ttcttttgca gtacgcacgt
     5941 ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca agtaatgaac
     6001 gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta tgcagctggt
     6061 aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa aaacctcttt
     6121 tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga cggttcgtgg
     6181 aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca aatgaatcaa
     6241 tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag aagctgcgca
     6301 aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc ttatggtaga
     6361 tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa aatgagtcgc
     6421 gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt atcaccacat
     6481 acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa aatctaatca
     6541 cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg ccagaaggaa
     6601 ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca agaaatgaag
     6661 aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag atgccactgg
     6721 atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc atcaactggc
     6781 agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa gcagcaaacg
     6841 gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt atcttttttt
     6901 tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat taagtaacgg
     6961 aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa aagcctatgc
     7021 cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg atccattgtt
     7081 tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg ttgctccagg
     7141 agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt tcccagaaac
     7201 cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa aacgagaatt
     7261 gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg aagccatcgc
     7321 gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt tacgtttatg
     7381 gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc aacctggaaa
     7441 gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt ggctgattgg
     7501 aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg atggcagttt
     7561 ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc aagaagaaat
     7621 caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga caaacaaagg
     7681 gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag agttaacgaa
     7741 ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc ttgaagttac
     7801 gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt tgcagacaaa
     7861 gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc aaggattgta
     7921 cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag aaaatgagca
     7981 tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag aagaagtaca
     8041 gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc aagaacatca
     8101 tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc tttccaaatt
     8161 acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg ctgcagatta
     8221 tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa aaagcatcgg
     8281 aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa aaaagaaaga
     8341 agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac tgaaaagtat
     8401 ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca cagcaatgaa
     8461 aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag aagtagaagg
     8521 aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga agcattacat
     8581 cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt gtaaggcagt
     8641 agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag ggtacttgag
     8701 aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca aaaaattcat
     8761 cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt cagatgagat
     8821 tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag gaagatgtcc
     8881 aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct tgaatccgga
     8941 atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa cgaatctcag
     9001 agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg gtaaaaaagg
     9061 gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt cttttccatg
     9121 gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa gaaaaaataa
     9181 tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt ccgaaaagtg
     9241 agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa cgttggggta
     9301 ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca ttcattttta
     9361 aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct aaagtatcct
     9421 gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc accaacgttg
     9481 attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca taaaacgaca
     9541 agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc ggctatgatc
     9601 attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg ttaagaatac
     9661 gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtcattttt tcgtaagaaa
     9721 aagcaaaata aaagtatata cattgattaa aagtatatac ttggatataa tgtacttact
     9781 atagaatgag aatggtatag agggtggcga aatatgaaaa aaatgaaaaa gtataaaatt
     9841 agaaaatctg gcaatagcga tgtaacaact atcccaccag aagtaaaaga attcatgggt
     9901 gtgcagacag gtgacgcaat cagttatgtt tttcaatctg atggttctgt acgaatgatc
     9961 aaagcacaag aagaacctga tattgattca ttagttgatt cgatcatgaa tcaatatgaa
    10021 gacgcgttaa aagatttggt tgatttatga ggtatctttc gactaaagaa attatcaaaa
    10081 tcaacgcgaa agttatttta ccgtcactcg ccaggcgaaa tgatcggtgt aaaagatgca
    10141 aatgcattag atatggccgt caaacagcct tcacaagcag tattcaatca agaactctat
    10201 cctgaaatct atgaaaaagc ggcgatttta gcaattaatt tagctaaaaa acatcctttt
    10261 cataatggca ataaacgaac tgctcttgtt gccatgttgt tgtttcttca attgaataat
    10321 tgtccagttg ctttttctcg tcaagaagct gttgatttta tcataatgat taccactagt
    10381 tcattggatt tcgattcact aaagtccaag ataaccagtt atttacgaca aaccgcaata
    10441 aaatgaacga attaaaaact ttatttctca attagatcag catgtcatta aggaatatgc
    10501 tgattttttt atgtttaatc atacaaaata gttttttcat aaaaatatgg aaaatatgga
    10561 ttaattttga aataattgat ttaaaa
//
LOCUS       DAZABD010000085        10546 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000085 DAZABD010000000
VERSION     DAZABD010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10546
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.086"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(52..1518)
                     /locus_tag="ACHALG_002455"
     CDS             complement(52..1518)
                     /locus_tag="ACHALG_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFN2587812.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
     gene            1905..2501
                     /locus_tag="ACHALG_002456"
     CDS             1905..2501
                     /locus_tag="ACHALG_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFN2587813.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(2559..3041)
                     /locus_tag="ACHALG_002457"
     CDS             complement(2559..3041)
                     /locus_tag="ACHALG_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587814.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(3147..3344)
                     /locus_tag="ACHALG_002458"
     CDS             complement(3147..3344)
                     /locus_tag="ACHALG_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587815.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            3639..4397
                     /locus_tag="ACHALG_002459"
     CDS             3639..4397
                     /locus_tag="ACHALG_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFN2587816.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            4457..4885
                     /locus_tag="ACHALG_002460"
     CDS             4457..4885
                     /locus_tag="ACHALG_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587817.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            4931..5003
                     /locus_tag="ACHALG_002461"
     tRNA            4931..5003
                     /locus_tag="ACHALG_002461"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4964..4966,aa:Gln,seq:ctg)
     gene            complement(5153..5524)
                     /locus_tag="ACHALG_002462"
     CDS             complement(5153..5524)
                     /locus_tag="ACHALG_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFN2587818.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            5739..5924
                     /locus_tag="ACHALG_002463"
     CDS             5739..5924
                     /locus_tag="ACHALG_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587819.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            complement(6007..6195)
                     /locus_tag="ACHALG_002464"
     CDS             complement(6007..6195)
                     /locus_tag="ACHALG_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587820.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(6219..6482)
                     /locus_tag="ACHALG_002465"
     CDS             complement(6219..6482)
                     /locus_tag="ACHALG_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587821.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            6640..6897
                     /locus_tag="ACHALG_002466"
     CDS             6640..6897
                     /locus_tag="ACHALG_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HFN2587822.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            complement(6968..7159)
                     /locus_tag="ACHALG_002467"
     CDS             complement(6968..7159)
                     /locus_tag="ACHALG_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587823.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(7300..7620)
                     /locus_tag="ACHALG_002468"
     CDS             complement(7300..7620)
                     /locus_tag="ACHALG_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587824.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(7643..8077)
                     /locus_tag="ACHALG_002469"
     CDS             complement(7643..8077)
                     /locus_tag="ACHALG_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFN2587825.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            8412..9050
                     /locus_tag="ACHALG_002470"
     CDS             8412..9050
                     /locus_tag="ACHALG_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFN2587826.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPV
                     QAEQPVVQETVQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTAT
                     NGRYIGRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(9374..9457)
                     /locus_tag="ACHALG_002471"
     tRNA            complement(9374..9457)
                     /locus_tag="ACHALG_002471"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9421..9423),aa:Leu,seq:cag)
     gene            9985..10485
                     /locus_tag="ACHALG_002472"
     CDS             9985..10485
                     /locus_tag="ACHALG_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN2587827.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
BASE COUNT         3355 a         2063 c         1694 g         3434 t
ORIGIN      
        1 aaaaaacagg aactgctgca aataagcaat tcctgttttt atgttcattg attaatgaat
       61 agagcccatt agttttttga ttggttcttt cacagtaaat agcaagatac caccgacgat
      121 tgctactgca ccgataatac cgaagtaagc agattctgtt cccggtgtgt agaagcgtgc
      181 aatttgtgca ttgattgctt gtgaagaagc atcagctaag aaccaaatag cgattgtttg
      241 tgattcaaat gctcgtggtg ctaattttgt tgtcacagac aaaccaactg gtgaaagaca
      301 caattcagca ataaccacga tgaagaaact tcccaacaac cacaatggac ttacttttcc
      361 acttgtacca taaagcatac ccggcaacat caatagcaag tacgataatc ctgcaaataa
      421 aagtccaaga gagaatttca ctactgtaga tggttgacgt tcaccaagtt tcgtccacat
      481 cgttacgaat actggtgtca gaataaagat gaaaattgga ttcaatgatt gataccagct
      541 tgcttggatc gttacaccaa atagcgtaga ttgtgtacgt tcactagcaa ataaagctaa
      601 gatagatgaa ccttgttctt gtaatgacca gaaaatgatt gctgcaagga ataatgggat
      661 ataagcccat actcttggtt tttcttcagc agttacatct tttgaagtca gcattttgat
      721 aaagtaatac aacggtaaga taatccccaa tacactgatt gtattgacaa aaaagtcaat
      781 tgtcagatga ccagttagtt gtgccccgcc gaatactgca gcgataacaa caagtgcgat
      841 gatcaagtta cggatgaatc ctttacgttc ttcagttgtc aaaggattcg gcggtgttgt
      901 acccactccg tctaatgtct tgcgtccttg gatgatgtat tgtaataatc caaagaacat
      961 cccaatagca gctaaagaga accctaaatg atagttatat tcttgaccga ttgtaccaac
     1021 gattagtggt gctaataatg cccctaagtt gacacccatg taaaaaatcg aaaatcctgc
     1081 atctcttcgc aagtcagtct ttgaataaag atgtccgacc atgccagaaa catttggttt
     1141 caaaaaggcg gttccgataa taatcaatgc catcgaaaca aacaatgtac ttacaccaaa
     1201 tggtaaagcc aagacgatgt ggccaagcat aatgaaaact ccaccgaaga atacagtgcg
     1261 ttttgctcct aacactcggt cagataccca tccgccgacg atactagaca tgtagatcaa
     1321 agatccgtag atcgacatga tagctaatgc tgttgctcgt ggtaatccaa gtcccccgtt
     1381 ggcaactgta tcgtacatat aatagataag taatgcacgc ataccgtaat aactaaatcg
     1441 ttcccacatc tcagtgaaga acaaagttga caatcccttc ggctgcccga agaagccctt
     1501 gtcttgctgt ttttccataa tctatacccc aattctttaa attttcagaa aatattatac
     1561 atctttaccg aaaaatttaa ctaaaaaact tatttaagaa atataaacta attttctata
     1621 aaagcaagta atttttaatg gcaatatcat aaaaaaaaga agatagagat ttaataatta
     1681 tatatcagct gtttttttca aataagcaaa aattcctaaa acacaacaat gaaagcgata
     1741 actaagaaaa tccttacata acgatttttc ttattaaaat tgttttttga acttcgctta
     1801 ttttttgtga gcatatctgt ttgcttactt tttgtaaggt gctatacttc atttcgtaat
     1861 caattaaaaa aatgcaaaac gcaaatcaaa ggagaaattt tattatgaca acttttactg
     1921 acactttgaa aaaccgccgt tcaatctatc atctaggacg taatgtttca ctttctaatg
     1981 aagaattaac aactttgatc aaagaagcaa ttaaagaaag cccaactgct ttcaatgctc
     2041 aatcaacacg cgcagttatc cttttcggag atgcacatga aaaactttgg gaaatcactg
     2101 aagaagcact tcgcccattg actccagcag aagctttccc aaatacacaa aacaaattag
     2161 ctggtttcaa aaatggttat ggtacagtat tattcttcaa agatactgac gtggtaaaag
     2221 gtctacaaga acaatttgaa ttatatgcag acaacttccc agattggtca gaacaatcaa
     2281 acggaatcgc aacagctaat acttgggtag cattagttga taaaggattg ggcgcaaact
     2341 tgcaacatta caacccagtt attgacgaag cagttgcaaa agaatggaat atcccttcaa
     2401 actggaaact acgttcgcaa ttagtattcg gttcaccaga aacaccggct ggggaaaaag
     2461 aatacatgaa cgatgccgat cgcttccgcg tatttggata attttatttg aaataaaaaa
     2521 cagaggttgt gacaaacatc ttgtcgcaac ctctgttttt attctggatc aatcataata
     2581 tcaatctctg cctcatcgtt taagcgttgg gacaaaaatg gaactaattc ttctaatgat
     2641 gcagaaaatt tcagcatcgg tcctcgccat ccgtttcgga aaaaattcat ttttacgatt
     2701 ttagaacctt ttggctgata taatacaatt ttattgatct cagaaaaagg aacacctttt
     2761 ttgtcaaagc tattcaaaac aagtccttct tctgtgattc ctctactgtc caacaaaaaa
     2821 ctaataaaaa tcattgctga caagatccct tttgccaatt catctgctgt aggatgactg
     2881 atgagtgtat accaaatgac tagtgacact aagaagggta tcaccaagta gacccatcga
     2941 tttcgcacag tccgataaat aatttctttt cgaaccagcc aaagaaataa aaacatcaca
     3001 acaacgaata aaaacaatag aaccgtaaag aatgttacca tgacaactcg cctcgctttt
     3061 tcttctccag aaaataggaa aagaatactg atttaccttc tcaaagaaat tggacttgca
     3121 gcacgccgca cgtccaattg ttttctttat tcaaatgtat catattttgc aacaaccatg
     3181 cttgcttttt catctaataa ttcttggtcc tctgcagcac attgggcaac tttgaccacg
     3241 gcgcagtttg tcatttttga tacaacttca ccaatgaccg tttttgtaaa accaattggt
     3301 ttgcatgcat attgttctcc taaattaatc atttcttccg tcatcaaaat cctcctaaac
     3361 tgctggaact tttctccaca ttaccactgc tatttctttg gagatttaac tcattgattt
     3421 ttatttacag ttaaaaatca accttacagg cattatagcg tactgttttc gttttcacaa
     3481 ttaaaacagc tgttgtattt agaagaattt ttttcaaaaa attaaaacat attccttaaa
     3541 aactaattat ttcctacata ttaaaatata atacttccat ttccaataaa aatacattta
     3601 taataggaaa gataaacagc tgaaaaggga gatttcaaat gaagaaaaaa acgttcaaaa
     3661 aactattgtt tctagggggc acagctgctc ttttattaag cggtacaagt tattacctac
     3721 atatccattc tccagaacat acaatgaaca ctgcacaaaa aagttcaaca caaaaaacga
     3781 aaaagacaaa agagaaagca gaagttttac tagatgttcc attagagagc cagtttgatt
     3841 ctccttcttt agaaaatggc tgtgaagtca cttcgctttc tatgctttta tctttttatg
     3901 gttatgaaac aactaaaaac cagttagctg agcaattgga ttacgtccct gttttcaatg
     3961 cagatgggac tcatggcgat cctaatgaag gatttgttgg tgatatcagt ggcggagact
     4021 gggctatggt cgtctatgtt cccccagttg cctcccttgc tcagcaaatc gtccaaacgg
     4081 attatcacac ttttccaaaa accgatgctc aactcgatga tatcaaaaaa gcactatcta
     4141 aaggaaatcc tgtctggact tctgtgacta tcaattttga agtcccagat gaaactgatt
     4201 ttatgacttg gaccacaaat aatggagaag taagagtcac tcctcttgtt catgcttgtg
     4261 tagtcactgg atatgacaaa gaaaatattt atgtcaacga tccctatggt gtaaaaaatc
     4321 gagcagtacc gatcaacgat ttttcggcga ttttcactgc aatgggtgga cagatgctaa
     4381 ctttggagaa aagctagtaa ttcaaacaaa aatcccttat actcaagata ccaaaccttt
     4441 gaagggagcg tagataatgg aaaaattcac acataaaaaa atggatccaa atgaaattcc
     4501 gattattttt gtccgtgact gcaagggaaa tgttcaaggt aaagtctcta ttaatgagtg
     4561 gaacgagcgt cgcagacctg ctaccctcaa tgaactggaa atcaaattgt atcgtcaatc
     4621 tcttgtttat tacgcagatc aagagtacga gaaagccacc gatttattga aatttctaat
     4681 tgctcgaaca gagtatactc gctttgaata tattgaacgt ttagcaaaca tctatcatat
     4741 tatgaatgaa cctgtcaaag aatatcagtt attggacacc gtcttgtcag tagcagaact
     4801 gattgccctt cctgctggat tagaaaagaa actagtacgc cgccttttac gagtaaagca
     4861 acaactatcc gatcaagaaa aataacttgc gtttttttag gaaagtgcta tgattatttt
     4921 gttgaataaa tgggatgtag ccaaactggt aaggcacttg actctgaatc aagcaattgt
     4981 aggttcgaac cctgccatcc caatttgatg tgcttgctga aaaaatgaac cccctgtaaa
     5041 agaacaatct ttttgcaggg ggattattgt attattttta ctacttgtat ttagtttaca
     5101 taattaaatt atgaataata aaatcgggcg tattcttaat acaaaaactg atttacccaa
     5161 taattgctaa tgctttctcc ggaacttttt ctctaggaac ttcctcatac gtttctatgt
     5221 gtgctccttt attgctaatt ttcaaataat gattttcttt taacacagaa agaccattaa
     5281 attcaaccgt tcgttctttc ccattggcat cagcagcttt ctgcacatac cttgctgtac
     5341 catattcatt tacttcctct ggtttttgtg tctttacata gacctctcct ttttcaacta
     5401 atggatttag ctgatcaagt ataccaggga tttctccata acttccttca gtataatatt
     5461 tcagtccaaa tagcaaaata ccactaatcg ccgccagtcc aatcaaatat ttaatcactt
     5521 tcatctttct acctccttgt tatatattca gtatatgggt cagttaaaga aattccatcc
     5581 gtcccaagtt ggagattttt ctcctacttt agaagcgaga atcgagtgtt tttttagtaa
     5641 aaatgatcgt tatttgatat tattgacgtg tggataaatc agtttttctc gttttgtcca
     5701 tgtaaaaaat catgttgata gcaaaggatg tgttcaaaat gaatcaagaa gaattatttc
     5761 aaaaagtcaa ggaaatgatt aagaacggca atttcgatgg cgcaaaacga tttatcgaag
     5821 aacataaaga acaattagga ccttataaag agaaagccca aaatctttta aaagatgtaa
     5881 atatcgacag tgtcaaaaac aaatttaaaa atctattcaa ataagtaaaa ctttcacttt
     5941 tgtaaaatca aaggaggaca atgctgctgc atcgtcctcc tttggttctt ttctgaatac
     6001 ttcatactaa aataaacgtg tcggatcgta atgataagtc acattgccgt tcagtgaggc
     6061 ttttcccacc acatatttac gattctccaa cttcagccac gcttctcgaa aagccattaa
     6121 ttcttctctt tttacatcaa tcttttcgat ttttccatcg atcaaatctt gaataagttg
     6181 ttcatatgga ttcatttttt tcctccatct gctgtcaatc aataagttgt aaaaataggt
     6241 tcatatgctt ttttggtatc tagatcttct ctgactttga ctgtcgtata aataattttt
     6301 tctactggga tactataata acttccttca ccattattga atcggtgaac gtctttcttt
     6361 gctaaaatac catttatttt attcgtcagt acacgttgtt cgacactgtt acttacaaca
     6421 tagtaaatca cccgaatatt attgatcaaa tgcaaatcga tttctaacac tacatgatcc
     6481 atgaaaatcc cactccttct ttcactttga ttatagcaag tttctcctct aatttcaaaa
     6541 tcgatacaga ttttaccgga taaatccaca aaactgcttg cgccatttcg cacaaagtgt
     6601 tattttgtat catggacttt atttatcgga ggcaatagta tgatcgcgat cggtcaattt
     6661 gttttttata ttcctttttt tattatgata agcattcttt tctattacat aaaatggaca
     6721 aaaaagaagt tttcagtttt actggcctct ttacctgctg tatattttac ttatcaaatt
     6781 ttttctttta ggcattggga aacaacgtct gtattaataa cacacatcat cgaattgacg
     6841 ctttcagtaa tttttcttat tatttggatc tattttttat ataagaatca gaattgaatc
     6901 cctcgcatgt ttaaaaagaa aacgattaaa gatgcagcaa atttctgccg atctttaatc
     6961 gttttttcta ttctgttata ccaaaaaaat gtttgaatcg gtgaaggatc gttccttgtt
     7021 ttaattctat aaattcttgt gcgatcgggt acttttcttc caaattttct ttgttctttt
     7081 cgatcaggct tttcgcttct tcatattttt tatctgcaca tagtgctttt acttcctgta
     7141 atacttttcc tgcttccatt tgcctcaccc tcttgccatt ttattgcttt aagttttatt
     7201 taagttatac gcattattct agtttcatcc caaataacct tttttcattt ttactcctcg
     7261 aactaatcag ttttctaaag gctgattggt tcgtcttttt tatttttcct ccatttcatt
     7321 ctgctcttct gttaatttat ctaatgcttt gattgatgct gcggctcttg cataatcata
     7381 gctgtataat tccataaatt tatcaggcag aacaaaccac gctggatgat aaccgttagc
     7441 tgtgacaggg aatcctttat cttgttggtt atctccatcc catctcagag caacaccttt
     7501 ttttcctttc cactttacta aagcgatcga gtatcccttc tttgtttgtg tgccgtcttc
     7561 aataattgct aaaatttcta ttttttcttt tggcgcacgg acttcttgag gacgaaccat
     7621 tttctattcc tcccttatct cattacttca cgaccaacac actgcaaggg gcatgtgata
     7681 cgacataaga agcggtcgat ccaactaatg cttgttgtat agcacctttt cctgtcgctc
     7741 ccataacgat caaatcaata ttttcttgtc cagggatgac attggcaatc aaatttcttg
     7801 gatctccagt ttcaactatt gcgtggatct cagcaatgcc gtactctttg gcatcattga
     7861 tttttttcag catggctact tctgttcttt gtttttcatc tgcatagatt tttgaaaagg
     7921 cataggcact atgggtcagc tcaactttgt tgatgattga cacgataaat aagtgggctt
     7981 cattgtcctt tgctaagtca agggcttcag cgaacgcttt gatcgattgt cttgatccgt
     8041 caactgctac catgatgttc tggtatcttt tactcattgg aaccgcctcc tttttctttt
     8101 attgtaacat ctcaagcaaa aatacaaaaa atatacgcaa tatgtaaata aaatgatgaa
     8161 caaaagcaat ttctttcaag ttatttatac atttcttttc tcatcagaac acctgttcta
     8221 atcccattat ttctgcatta tgtgaaattc ctcatcattt ttcacatcga gcgaacatca
     8281 ttctgttaca aaaaacgtgg atttgtcata aattgtcatg cttcttttac ctatgaaata
     8341 ttttcacgtg ttaaagtatt cctcgttgaa cacaacaaat aaaacttttt aacgaataaa
     8401 ggagattttt catgacatca cttaaaactt tactttttgg aacaactttg gctgccggcg
     8461 ctgcattctt catgggaacg actgctcatg cggacgaagc ttataccgtt caatcaggtg
     8521 atactctatc aacgatttct caaaaatacg ttggtgataa ctccttgatc aatgccattg
     8581 cagaatcaaa ttcaatttct gatattaacc tgatctactc aggacaacaa ttaacaatcc
     8641 caacagaagg ctctgcacaa gcagctgctg aacctcaagc agccgttcaa gaagcaccag
     8701 tccaagctga acctgttcaa gctgaacaac ctgttgtaca agaaacggtt caaacggaaa
     8761 cacaagcagc gcctgttgct gaaacacaac ctgctcctgc tgtaacagaa actgctgcta
     8821 ctccagcaag tacaagctct gcaaaagaat ggatcgcaca aaaagaatca agcggttctt
     8881 acacagcaac gaacggacgt tacatcggcc gttaccaatt agattcttct tacttaaatg
     8941 gtgactactc tgctgcaaac caagaaagag tagcagaaca atatgttact tcacgttatg
     9001 gttcttggga agcggcgaaa gcattctggg aagcaaacgg ttggtactaa gattcattat
     9061 ttgaacgttt aaacacattc aaaatatagt aagaggtcat gcgatttatt ctttcgcatg
     9121 acctcttttt gagtttaagt ttagtcttta ttccttaact atcgtttgcc agtatttctt
     9181 gtatcagaat ctataaatct actatttgaa tcaatctatt aggttttaaa ctgtcccacc
     9241 ttcacacaaa ttttttttga tttatttgaa tctggttccg ttgtttaaaa tttttcctat
     9301 attcttttag ataatatgtt attttctttc tcatcgctcc aggtgtacct tctctttata
     9361 aaaaaacaaa ttatgcggct aagaagactc gaacttccac gaggtcaccc tcactagcgc
     9421 ctgaagctag cgcgtctgcc aattccgcca tagccgcatc acatctttta gtttaggatt
     9481 acatgtgttt tttgtcaaga acatattttt gttctttttc atttctgtac gccctccctt
     9541 atgccatcta cacaaataat tcatccgagc aactccatca tctattgagg gttggtgaaa
     9601 aaatctgttt tatccatatg atcgatttca atgttaaacg aacgactctg ggattagtaa
     9661 ctattttatt tttttcttca tttctttttt cctctttttc tcgattcagc tatcttcttt
     9721 catttggata ttgattattt cttctgttga ttactatcct tctggaattc tagacgctga
     9781 agtaattcac gttttctttc ttctagagat cataagtaac gaatgaacga acttcaaata
     9841 atttttttat tgtaattcat atttgtattg ttcgatgatt cttaatacga tatgacacaa
     9901 taaataaaaa taggcatggc tatttacaac aatagtgcta tagtagaaaa caaatgaata
     9961 aggttactta aagaggtgaa tacgatgaaa ctgatcaaac tatgggaagc agatttaaag
    10021 aaagcttatc agctacaaac tagctttgga ccaaatgaga gtggatttat caacaatgct
    10081 tatggctata catttgctga atttttagat tatgtaaaaa aatgcgaaaa aacgtcttta
    10141 ggaatcgatc ttcctgagag ttacgttccc gcaaccgtct ttattttaga aagtgatgaa
    10201 catgagtatg taggaatttt taatttgcgc cataaactga acgaaacatt aagaaacggc
    10261 gctggccata ttggttacgg catctcccct aattatcgta aaaaaggata tgccacacaa
    10321 ggattagcat tagtactaca agaggcaaaa aaaatcggca ttgatgaggc acttttgtct
    10381 gtaaataaaa aaaatcttgg cagtcttaaa gcacaacaaa aaaatggggc tgcgatttat
    10441 cgtgaagatg agaaagaata ctatacgaaa atcaaactga tgtaaaaaag aaggattcca
    10501 aaaggaaagg gatttgaatg tcctttatca atagaacaca tttatt
//
LOCUS       DAZABD010000086        10497 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000086 DAZABD010000000
VERSION     DAZABD010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10497
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.087"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..461)
                     /locus_tag="ACHALG_002473"
                     /pseudo
     CDS             complement(1..461)
                     /locus_tag="ACHALG_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
     gene            complement(713..1072)
                     /gene="rplT"
                     /locus_tag="ACHALG_002474"
     CDS             complement(713..1072)
                     /gene="rplT"
                     /locus_tag="ACHALG_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFN2587828.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(1125..1325)
                     /gene="rpmI"
                     /locus_tag="ACHALG_002475"
     CDS             complement(1125..1325)
                     /gene="rpmI"
                     /locus_tag="ACHALG_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFN2587829.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(1397..1921)
                     /gene="infC"
                     /locus_tag="ACHALG_002476"
     CDS             complement(1397..1921)
                     /gene="infC"
                     /locus_tag="ACHALG_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFN2587830.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(1965..2090)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            2241..2873
                     /locus_tag="ACHALG_002477"
     CDS             2241..2873
                     /locus_tag="ACHALG_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFN2587831.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGNQSYLDAKN
                     MVLRESHIQKLTMQKTGLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTTIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(2875..3780)
                     /locus_tag="ACHALG_002478"
     CDS             complement(2875..3780)
                     /locus_tag="ACHALG_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFN2587832.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWEIALIISLVLSIWMLIALWRRIR"
     gene            complement(3865..4524)
                     /locus_tag="ACHALG_002479"
     CDS             complement(3865..4524)
                     /locus_tag="ACHALG_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFN2587833.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     CVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVS
                     I"
     gene            4840..5784
                     /gene="mvk"
                     /locus_tag="ACHALG_002480"
     CDS             4840..5784
                     /gene="mvk"
                     /locus_tag="ACHALG_002480"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HFN2587834.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            5777..6754
                     /gene="mvaD"
                     /locus_tag="ACHALG_002481"
     CDS             5777..6754
                     /gene="mvaD"
                     /locus_tag="ACHALG_002481"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HFN2587835.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKKGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            6767..7852
                     /locus_tag="ACHALG_002482"
     CDS             6767..7852
                     /locus_tag="ACHALG_002482"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HFN2587836.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            7849..8913
                     /gene="fni"
                     /locus_tag="ACHALG_002483"
     CDS             7849..8913
                     /gene="fni"
                     /locus_tag="ACHALG_002483"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFN2587837.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKDPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAQEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(9162..9965)
                     /locus_tag="ACHALG_002484"
     CDS             complement(9162..9965)
                     /locus_tag="ACHALG_002484"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFN2587838.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIRDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(9985..10308)
                     /locus_tag="ACHALG_002485"
                     /pseudo
     CDS             complement(9985..10308)
                     /locus_tag="ACHALG_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
     gene            complement(10341..10478)
                     /locus_tag="ACHALG_002486"
                     /pseudo
     CDS             complement(10341..10478)
                     /locus_tag="ACHALG_002486"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
BASE COUNT         3107 a         2220 c         2008 g         3162 t
ORIGIN      
        1 aaatacctaa tgacatcaag atcggcaaca caataactgc aatagaaatc actggtccga
       61 ttcctgtcat cgacatgaat ataacagcgg ttacaataca tagtaagctc atcgtgattc
      121 ttggcttgtc ccctccaagt tcaactacct ttcgaatcag ggtagctgcg attccgctat
      181 ctactagaac acgcccgaaa aaggcaccaa agaagacatt taccaagatc gatttggcgt
      241 actcagctgg ccccgtcgta tagatatgcg tcaacacatc aattactcct tgcccttcca
      301 tcgccgagtt tggtgaaaac gtgtttccga ctagcgctaa tcccatccat acagtagcca
      361 tgatgacaaa agccgaccat caagttgtag ccttttacac agtaccagac catgaaaaag
      421 aatgagataa ctaacaaaat accaataata atatcactca tgttattcct ccgtccattt
      481 tgtttttctg ataagcaaac taatatatca gtcaaactca ttctaactta tgtagttagc
      541 gctttcatta aaggatgacc ataaatgttc agttttcaat taaaaaacct taattatgtt
      601 attttagact tatacgttat tgtcctgttt catttgatgg aggattgtaa ataaaaaaag
      661 aggaaagaac ataacttttc gttatcttct ttcctcttta taattgttta gcttatttag
      721 ccaaagcttc ttttgcttgg tcagccaatg cagtaaatgc tgctgcatcg ttaacagcca
      781 agtcagctaa cattttacgg ttgatgtcga tttcagcaac tttcaaaccg tgcattaatt
      841 ttgagtagct caagccattc atacgagctg ctgcgttgat acgagcaatc cacaatttac
      901 ggaagtcgcg tttcttttga cgacgatcgc gatatgcgta gttgtatgaa ttcattactt
      961 gttcttttgc tgttttaaat aaagtgtgtt ttgaaccgta gtaacctttt gctaatttta
     1021 gcactctttt acgacgttta cgagttactg ttccaccttt aacacgtgcc atgtataatt
     1081 cctcctaata aaaataaatc ttcagattct gttatttgta cttcttattt catttttgct
     1141 agttgttggc gaatacgttt gaagtcgcca ctagaaacca tgcttgcttt acgcaattga
     1201 cggcgttgtt tttttgtttt gccgtggaaa cggtgacttg taaacgcgcg gaagcgtttc
     1261 aatccgcctt taccagtacg tttgaaacgt tttgctgatc cgcggtgtgt tttttgtttt
     1321 ggcatgacta tatttcctcc tcaaaatctt tcgttatccg actatcggct gtccaacagc
     1381 cagttagtca ttcaaattac ttactgtcgt ttttcggtgc cagcgtcaag aacatgctgc
     1441 gtccgtccat tttcgctttt tgttccactg ttgcaatatc cgcagtttct tcagctaagc
     1501 gattcaagac tttttgacca atttctttat gggtaatggc acggcctttg aaacggatag
     1561 aagctttcac tttatcgccc ttttccaaga acttacgtgc attacgaagt tttgtattga
     1621 agtcattcaa gtcgatcgta ggacttaagc gaacttcttt tacattgatc actttttgtt
     1681 ttttacgcgc ttcgcggtct ttcttttgtt gttcgaaacg gtatttaccg tagtccatga
     1741 ttcgcgcaac tggtggtttc gcaccagggg caactaacac aagatccaag tttgcttgtt
     1801 ctgcaatatt caatgcttct gcttttgtct taactcctaa ttgctcacca tctgaaccga
     1861 tcaatcgtaa ctctcgtgca cgaatgccgt cgttaaccat catatccttt gctatggtca
     1921 ttcacctcca aatattatga gagaaagaaa agcttaccga ataaacggca ataaaaaaac
     1981 gagttctctt acagaacccg cgcgtacttc acctaataga accagtgaaa actatctagc
     2041 ccagtgacat tatcaactaa ggcgagaagc gggtgcttct gcttttattt ctcaaccatt
     2101 atagtatagc aaaaactaat ctttttgtca atgtttttca tacagtttgt cttccttttc
     2161 tgaaaatgat tagattagca ggctttctag cagtgattcc cgcttctttg ctatactaaa
     2221 gaaaaacgag gtgaacccac atgaaaaaaa tcgaacctat tccaccttct cttcctcttc
     2281 ttacaaaggg agagttttat ttagaagatg aggagatctt ctctggtctt ctgatcaacg
     2341 gaggtaacca aagctatctc gatgccaaaa atatggtatt aagagagagt catatccaaa
     2401 aacttacgat gcaaaaaaca ggtttacgtc attttgaatg cagtaatgtc atttttgaaa
     2461 aatgtgattt ttccaatctg gagtggattg gtggcagttt ccaccaagtc gtgtttcatc
     2521 aatgtaaatt aacaggtacg aattttgccg aaagttatct tcgagactgt actttcactg
     2581 attgtattgc ttcgatggct tcctttagta gtacaaactt gaaagctgtc tccttcgatc
     2641 attgtcaatt agaagacagc gaattttatg aggtcacttg gaaaaacctt tttttgtctg
     2701 acaatcagtt aaccggttcg aactggtttc gaacttctct gaagggatta gactttacga
     2761 ctaatttctt cacaacaatc gctttatctc aagattcttt ggcgggacta atcgtcaatc
     2821 aagagcaagc acttgtgatc gcccaagccc ttggtttgca gatcaaagac tagatcaacg
     2881 aatccgccgc cacagtgcaa taagcatcca aatagataag accaaactga tgatcaaggc
     2941 gatttcccat ccgaaaacgg aatgggtaaa gggtaactgg agattcattc cgaagaatcc
     3001 tgtcacaata gttggaacgg tcaagatcag cgaccagacc gtcaaaaatt tcatcgtgtc
     3061 attcaagttg ttattcagca aattattata agtgccagag agctggtcta aaatctgcga
     3121 agctaagttc gtcatttcaa ctgcttgtcg tgcttcgatc agtgcatcgt ccaactcttc
     3181 tttttccttt tctgacaact tgcgataaat agccagtgcc ttgatttgtt ctaaaagtac
     3241 tacattttgt tttgttcctg taactaagta aaccaaaccg atctctaaat ctgaaagttg
     3301 cagcagattc ttgttcgttg ttttttctct taatttcaga ttaagccgct gacgttcact
     3361 attcacttcc tcgattaaag gaaaaaagga atcagaaatc aaaaacaatg tacgaaacaa
     3421 taaactgtat acactcatgt ctggcatccg ttccaaaagc gatcgaatta gtcgaatcac
     3481 gtactcggta ttcggcgtgg taaatgtaaa caaaccgtct tcttttaaaa taaaagcgat
     3541 aggactagtt tcataatgat tatcgacttt ttgctggtgc ggaacatttg atacaagcag
     3601 taacgcttca tcaaaagaat cgtactctac gcgcgcccgt tcatttttat ctagagaata
     3661 agtcagcatc tcgtcactaa tgccatattc ttcttttaag tttttaacag ctagcacatc
     3721 gttttgctgt acattgatcc aaaaattacg gtcttgatta aaatagtgtt tttcccgcat
     3781 actctatcct cctgacattt gtttccagta taaagaaaaa agtcgctcaa ataaaggttt
     3841 accctttatt tgagcaactg taaatcaaat ggatactttg gtttggcgaa tctctgctaa
     3901 tttttcttcg atcattttca agacttcttc ttttgcttga ggatcttcca caaaattata
     3961 gcggtctcca tcgatttgga tttttggact ttctttgtat tcttcatacc attgatcata
     4021 acgtctgttc aattctttgt aataatcgta tagacttggg tcaaaggaca attgttcata
     4081 cgaacgacca cgtttttcga tacgctctaa catcgtatca aaagatacac gaatatgaac
     4141 taataaatcc ggatgttttt tatgggcagc atagggaagt tcttccatca tgttttccag
     4201 cagttcatca tacacacaaa cttctgtttc tgttgctctt cctagatctg catttagatg
     4261 gaaaagcagt gaatcttcat aaatcgaacg gtccaataca ttattgtcat ctttcattgc
     4321 ttgtttgatg cttgcaaaac gtttattcaa aaaataaatt tgcagcaaaa aagcatactg
     4381 ttcagggttt gcataaaata agggcaagac ttcattatca tcaatcgatt cataaaatgc
     4441 ctgactatca aagtgttcag ccatcatttc tgttaaactt gattttccgg caccaattgt
     4501 acctgctaaa acgatcacgc tcatttacct ccagctcact tcatcactaa atattgtgtt
     4561 taactccaac cttttttaaa tataccccca atatctagta gtgtcaattg tattttttct
     4621 agtcaaagaa gaaaaaaagt caaaaaattc ttaagaaaaa agtgtttttt ctttagaaaa
     4681 cgtattctta gttttacttc tgagatggtt ttagtaaaat aaaggaggaa tgttttttga
     4741 gacccttagt ggacaaaaaa attcgtgaag cagcgcaact cgttttgggc gctttatttt
     4801 ttgcttaaaa atcactttta cgtgagaagg agttagatta tggcaaacta tggccaagga
     4861 gaatcaagcg gaaagatcat attgatgggc gagcacgcgg ttgtttatgg agaaccagcg
     4921 attgcctttc ctttctatgc aacaaaagtc accgcattcc ttgaagagct ggatgcaatg
     4981 gacgatcaac tggtttcttc ctactattca ggaaatttag ccgaagctcc tcatgcatta
     5041 aaaaatatca aaaaattatt cattcactta aaaaaacagc atgacatcca aaaaaacttg
     5101 caactgacca ttgaaagcac gattcctgct gaacgtggaa tgggatcaag cgctgcagtc
     5161 gccacagcag tcactcgtgc tttttatgat tacttagcat ttcctttgtc tcgtgaaata
     5221 ctattagaaa atgtccagct ttcggaaaaa atcgcccacg gtaatcctag tggaatcgat
     5281 gcagccgcta ctagcagctt gcagccgatt tattttacaa aagggcatcc ttttgactac
     5341 ttttctttga acatcgatgc ttttttgatt gtcgctgata caggaatcaa aggacaaaca
     5401 agagaagccg tcaaagatgt cgctcacctc tttgaaaccc agcctcatga aactggacaa
     5461 atgatccaaa aattaggata cttgacgaag caagcaaaac aagcaatcat tgagaattca
     5521 ccagaaacgt tggcacagac aatggatgaa tcacagtcac ttctggaaaa gctgacaatc
     5581 agcaatgatt ttcttaatct attgatccaa acagcaaaag ataccggggc attgggtgcc
     5641 aaattaactg gcggtggacg cggtggctgt atgattgcat tagcacaaac aaaaacaaaa
     5701 gcccaagaaa tcagtcaagc acttgaagat gcgggtgctg ctgaaacttg gatacaagga
     5761 ttaggagtac atacctatgt ttaaaggcaa agcacgcgca tataccaaca ttgctctaat
     5821 caaatattgg ggtaagaaaa atgaagaact catccttcca atgaacaaca gcctttcgtt
     5881 aacattggat gcattttata cagagacaga agtcatcttt tctgatagct atatggtaga
     5941 tgaattttat ctagatggca ccttacaaga cgaaaaagca acaaaaaaag tcagtcagtt
     6001 tcttgacctt tttcgtaaag aagccggcct ctcactaaaa gcttcagtaa tcagtcagaa
     6061 tttcgttcct accgcagctg gattagcctc ctctgccagc gggctagctg ctttagcagg
     6121 agcttgcaat actgctctta agcttggatt agacgatctc tctctttcaa gatttgctcg
     6181 acgcgggtct ggttcagctt gccgaagtat tttcggtggt ttcgttgaat gggaaaaagg
     6241 ccatgacgac ttaagttctt acgctaagcc agtcccttcc gattctttcg aagacgattt
     6301 agcaatggtt ttcgttttga tcaacgacca gaaaaaagaa gtgtccagca gaaatgggat
     6361 gcgtcgaaca gtcgaaacat ccagttttta tcaaggctgg ttagattccg ttgaagggga
     6421 tctatatcaa ttgaaacaag caatcaaaac aaaagatttc caacttctcg gagaaacgat
     6481 ggaaagaaac ggactaaaaa tgcatggaac gactttagct gcccagcctc cattcactta
     6541 ctggtctcct gacagcttaa aagcgatgga tgcggtgcgg caattgagaa aaaaaggcat
     6601 tccatgctac tttacgatgg atgctggccc taatgtcaaa gttttagtgg aaaacagcca
     6661 tttatctgag gtacaagaaa catttaccaa attatttagc aaagaacaag taattactgc
     6721 ccatgctggt ccaggaattg cgattattga ataaagggag atttttatga ttgaagtatc
     6781 tgcaccaggc aaactctata ttgccggaga atatgcagtt gttgaaacag gacatccagc
     6841 agttatcgct gcagtcgatc aattcgtaac agtcactgta gaatccgcac gaaaagtcgg
     6901 aagtatccaa tctgcacaat atagtgggat gcctgtacgt tggacaagac gcaacggaga
     6961 attagtttta gatattcggg aaaatccttt tcattatatc cttgctgcta ttcgtttgac
     7021 tgaaaagtat gcacaagaaa aaaacattct tttatccttt tatgatctga aagtaacaag
     7081 tgagttagac agttcaaatg gccggaaata tggattgggt tctagcggtg ccgtgaccgt
     7141 tgcaacggtc aaagccttga atgtttttta cgcgttgaat ttatctcaat tggagatttt
     7201 caagattgca gcactagcca atttagcagt tcaagataat ggttcctgcg gcgacatcgc
     7261 tgctagctgt tatggtggct ggatcgcttt ctcaaccttc gaccatcctt ggctccaaga
     7321 acaagaaact cagcattcta tcagtgagtt acttgccctg gattggccag gtctatccat
     7381 tgagccattg attgctcctg aagatttacg tttattgatt ggttggacgg gtagccctgc
     7441 ctctacttct gatttggtcg atcaagttca ccgttcgaga gaagacaaaa tggtggctta
     7501 tcagcttttc ttaaaaaaca gtacagaatg tgtcaacgaa atgatcaaag ggtttaaaga
     7561 aaataatgta acgttgatcc aacagatgat tcgaaaaaac cgacaattac tgcatgattt
     7621 atctgcaatc actggggtcg tcatcgaaac gcctgctttg aacaaattgt gtaatttagc
     7681 tgaacagtat gaaggagccg caaaatcttc tggtgcaggt ggtggcgatt gcggaatcgt
     7741 aattgttgac cagaaatctg gcatccttcc tttaatgagt gcatgggaaa aagcagaaat
     7801 cactccactg ccgttacatg tctatagcga tcaaagaaag gaaaaccgat gaatcgaaaa
     7861 gacgaacatg tttcactagc taaagccttt catgataaac aaaaaaacga atttgacttc
     7921 gtacggatca tccataaccc tttgccgcaa atagcagttt ctgatgtcga tctaagtaca
     7981 caggcagttg ggtttacact aagcagccca ttttatatca atgcgatgac tggcggaagc
     8041 gaaaaaacaa aaaaaatcaa ccaagatctt gcaatcgtcg caagagaagc agatttgatg
     8101 attgccactg gttcagtcag cgctgccttg aaagaccctt ctttagccga tacttatacg
     8161 attatgcgac aagaatatcc tcacgggaaa atcattgcca acatcggtgc agggacttct
     8221 gtcgaaagag cccaagaagc tatccgattg tttcacgcag atgccttaca gattcatttg
     8281 aatgcacctc aagagttggt tatgcctgag ggagaccgtg attttaccaa ttggaaagta
     8341 cttattcaag agactcaaac agccattgac gtccccctca tcgtaaaaga agtcggtttt
     8401 ggcatgacaa gagaaacgct caatgatcta gctgctttag gagttcatac agttgatatc
     8461 agcggtcgaa gcggaacaag cttcacccag atcgaaaacg cccgtcgctc caagcgggaa
     8521 ctgagttatt tagctgactg ggggcaatcg acagtctctt cattgcttga agcaaatgaa
     8581 gcagacactt ctatggaaat cctagcctcc ggaggtatcc gcaatgccta tgacatattt
     8641 aaagcccttt gtctcggcgc aaatgcagtg ggtacttcag gaaccgtact gacgcatctg
     8701 atgaatcacg gcgtagaaga aactattata ctgatgaagc aatggcaaga agagcttcgt
     8761 ctgctttata ctatggtcgg cgcaacgaat accgcggccc tacaccaaca atcactgatc
     8821 ttttccggac cagtgaagga ttggtgcgaa gcaagaggga tcgacttagt gaagtatgga
     8881 agacgcacag aaaagagcgt gggacaaaag taaagaacac ttttatccca cgctctaaac
     8941 acgtataaac ggcggaagca aaagcaactc cttcggaaat aagctgaaat tcacaaaaat
     9001 ttgaaaagca attttcgtga attccttctt atttcttgga gttaaacgct tctgtcccag
     9061 cctctttttt cgaataaatg atgtttaatc agctgtcctt cggtattcgt tcaagccgct
     9121 gatttcacaa cctctccact aaacggtgta ctaaagctct tttaaggttg ttttccgata
     9181 taggcaagta tgcctccgtc cacatacaag acatggccgt taacgaaatc agatgcgcta
     9241 gaagccaaga agacagatgg tccagccaaa tcgattggct cgccccagcg tgctgctggc
     9301 gtacgtccaa cgataaattg atcgaatgga tgacgttcac cgttttcttg tgtttcgcgt
     9361 aatggagcag tttgcggtgt agcaatataa cctggtccaa ttccattaca ttggatattg
     9421 tattgaccgt attcagaagc gatgtttttc gtcagcattt ttaatccgcc ttttgccgca
     9481 gcataagcac tgaccgtttc gcgtcctaat tcactcatca tggagcagat attgatgatt
     9541 ttaccgccgc ctttttcgat catatcccga ataactgctt ttgaaacgat gaatggggca
     9601 tttaaatcca cgtcgattac ttggcggaag tctgccgcat ccatttcgat catcggtgtc
     9661 cgtttgatga tccctgcatt gttgactaga atatcaactg aacctacatc ttctttgatc
     9721 tgttgaacca tcgcttgaac cgcttcttca tcggtcacat cgcagacata accttttgca
     9781 tcgatgcctg cttctttata atgcgctata ccttcatcaa ctgattcttg attcaagtta
     9841 ttaaatacga tttttgctcc tgctgtagct aaagagcgtg caatctcaaa tccaatacca
     9901 tagacagcac cggtgactaa tgctactttt ccatctaaac gaaacatatc catactaaat
     9961 tccatcattt tctctccctt atgcttattt taattgatcc atttgcacca tatccatgtc
    10021 tgtataggtg atattttctc cgcacattgc ccagatgaat gagtaattgc ttgtacctac
    10081 acctgaatgg atcgaccagc ttggcgaaat aatggcttgt tcattgtcta cgactaaatg
    10141 ttttgtttca tctggtttgc ccatcatatg gaacacacgt gtatcttcat tggcataatc
    10201 aaaatagaca taagcttcca tacgacgttc atgtgtatga caaggcatcg tattccaagc
    10261 acttcctggt tctaaaatcg tataacccat ttataaatga cagctatcgc tgaagcttct
    10321 actgctgtaa atatccccgt caagatacca ccgataatga tgacaatcaa tagaaggcta
    10381 ggaatcgcct cttttattat ttttccagca tgagccatct gtcctttttc gacaacaggg
    10441 tatttattct ttttagcaac gatgaatgct actagcatga ttcctagcgc ccacaaa
//
LOCUS       DAZABD010000087         9964 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000087 DAZABD010000000
VERSION     DAZABD010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9964
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.088"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            8..81
                     /locus_tag="ACHALG_002487"
     tRNA            8..81
                     /locus_tag="ACHALG_002487"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42..44,aa:Pro,seq:tgg)
     gene            101..173
                     /locus_tag="ACHALG_002488"
     tRNA            101..173
                     /locus_tag="ACHALG_002488"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:134..136,aa:Ala,seq:tgc)
     gene            201..274
                     /locus_tag="ACHALG_002489"
     tRNA            201..274
                     /locus_tag="ACHALG_002489"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:235..237,aa:Met,seq:cat)
     gene            288..361
                     /locus_tag="ACHALG_002490"
     tRNA            288..361
                     /locus_tag="ACHALG_002490"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:322..324,aa:Ile,seq:cat)
     gene            385..474
                     /locus_tag="ACHALG_002491"
     tRNA            385..474
                     /locus_tag="ACHALG_002491"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:421..423,aa:Ser,seq:tga)
     gene            487..560
                     /locus_tag="ACHALG_002492"
     tRNA            487..560
                     /locus_tag="ACHALG_002492"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:521..523,aa:Met,seq:cat)
     gene            579..654
                     /locus_tag="ACHALG_002493"
     tRNA            579..654
                     /locus_tag="ACHALG_002493"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:612..614,aa:Asp,seq:gtc)
     gene            656..726
                     /locus_tag="ACHALG_002494"
     tRNA            656..726
                     /locus_tag="ACHALG_002494"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:688..690,aa:Gly,seq:tcc)
     gene            740..813
                     /locus_tag="ACHALG_002495"
     tRNA            740..813
                     /locus_tag="ACHALG_002495"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:774..776,aa:Ile,seq:gat)
     gene            838..925
                     /locus_tag="ACHALG_002496"
     tRNA            838..925
                     /locus_tag="ACHALG_002496"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:872..874,aa:Ser,seq:gct)
     gene            947..1018
                     /locus_tag="ACHALG_002497"
     tRNA            947..1018
                     /locus_tag="ACHALG_002497"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:980..982,aa:Glu,seq:ttc)
     gene            1041..1130
                     /locus_tag="ACHALG_002498"
     tRNA            1041..1130
                     /locus_tag="ACHALG_002498"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1077..1079,aa:Ser,seq:tga)
     gene            1143..1216
                     /locus_tag="ACHALG_002499"
     tRNA            1143..1216
                     /locus_tag="ACHALG_002499"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1177..1179,aa:Met,seq:cat)
     gene            1235..1307
                     /locus_tag="ACHALG_002500"
     tRNA            1235..1307
                     /locus_tag="ACHALG_002500"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1268..1270,aa:Asp,seq:gtc)
     gene            1311..1383
                     /locus_tag="ACHALG_002501"
     tRNA            1311..1383
                     /locus_tag="ACHALG_002501"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1344..1346,aa:Phe,seq:gaa)
     gene            1388..1458
                     /locus_tag="ACHALG_002502"
     tRNA            1388..1458
                     /locus_tag="ACHALG_002502"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1420..1422,aa:Gly,seq:tcc)
     gene            1471..1544
                     /locus_tag="ACHALG_002503"
     tRNA            1471..1544
                     /locus_tag="ACHALG_002503"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1505..1507,aa:Ile,seq:gat)
     gene            1556..1627
                     /locus_tag="ACHALG_002504"
     tRNA            1556..1627
                     /locus_tag="ACHALG_002504"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1589..1591,aa:Glu,seq:ttc)
     regulatory      1816..1895
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SMK box riboswitch (SAM-III) riboswitch; SAM
                     riboswitches modulate the expression of downstream genes
                     in response to levels of S-adenosylmethionine; SMK box
                     riboswitch shares no known similarity to SAM-I and SAM-II;
                     Derived by automated computational analysis using gene
                     prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            1893..3086
                     /gene="metK"
                     /locus_tag="ACHALG_002505"
     CDS             1893..3086
                     /gene="metK"
                     /locus_tag="ACHALG_002505"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFN2587839.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3216..4688
                     /locus_tag="ACHALG_002506"
     CDS             3216..4688
                     /locus_tag="ACHALG_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFN2587840.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            4771..5559
                     /locus_tag="ACHALG_002507"
     CDS             4771..5559
                     /locus_tag="ACHALG_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFN2587841.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(5626..6294)
                     /locus_tag="ACHALG_002508"
     CDS             complement(5626..6294)
                     /locus_tag="ACHALG_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HFN2587842.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            6409..8121
                     /locus_tag="ACHALG_002509"
     CDS             6409..8121
                     /locus_tag="ACHALG_002509"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN2587843.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8124..9896
                     /locus_tag="ACHALG_002510"
     CDS             8124..9896
                     /locus_tag="ACHALG_002510"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN2587844.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         2900 a         1781 c         2278 g         3005 t
ORIGIN      
        1 aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa ggggtcgcag
       61 gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag ctcagctggg
      121 agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct ccatttcatt
      181 taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc ggttcatacc
      241 cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga cctttagctc
      301 agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc tacaaggtcc
      361 attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag ggaacggtct
      421 tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc tcctttttca
      481 tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga aggtcgtagg
      541 ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg taggggttaa
      601 catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc gccatgcggg
      661 tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg attctcgtca
      721 cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg cctgataagc
      781 gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat tggttttggg
      841 gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg aaaccggcgc
      901 gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc cgttggtcaa
      961 gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt acgggtcatt
     1021 aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa cggtcttgaa
     1081 aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct ttttcatatt
     1141 atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt cgtaggttca
     1201 aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg ggttaacatg
     1261 cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt ggctcggtag
     1321 ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt ctgtcccgcg
     1381 ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt gtcgcgagtt
     1441 cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt tagagcgcac
     1501 gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt ttattggccc
     1561 gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc gaatcccgta
     1621 cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga atgattttaa
     1681 aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga cagttctaat
     1741 gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag aaaactgttt
     1801 tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg gagatgcctt
     1861 gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta tttacatcag
     1921 aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat gcaatcttgg
     1981 atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct gtaacaactg
     2041 gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt caaaaagtcg
     2101 tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt gatggagata
     2161 cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa ggagttgatg
     2221 aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt ggtgctggtg
     2281 accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg cctttaccaa
     2341 tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca aatgaattaa
     2401 cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat caaggacaac
     2461 cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta gataatgaaa
     2521 caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct gaattattag
     2581 atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt ggtcctcaag
     2641 gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt tatgctcgtc
     2701 atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct gccagctatg
     2761 ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa gcagaagtac
     2821 agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat actttcggta
     2881 ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc gatcttcggc
     2941 cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa acagctgctt
     3001 acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac aaagtagatg
     3061 cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc caagtggtgg
     3121 ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt atttatctat
     3181 ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca aatgttactt
     3241 tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt acaatcgtaa
     3301 caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg aactgggtat
     3361 tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa cttgcggata
     3421 aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc ggttcgttat
     3481 tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt caagggatgg
     3541 gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat tctattgaaa
     3601 agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt gtttttggac
     3661 cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt tttatcaatg
     3721 tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa ccaaaaatca
     3781 tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc atgcttgtct
     3841 cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt gtttctgctt
     3901 tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa agagctaaag
     3961 atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc aacttggctg
     4021 cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg tggatgcaag
     4081 gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta tcactagtct
     4141 ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact cattgggtct
     4201 tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt atgcctttca
     4261 gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc ttaggtgtga
     4321 ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag cttggtgtag
     4381 caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt tctatttttg
     4441 gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta acaaatgatg
     4501 cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat gcggatattt
     4561 tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta gttggcttgt
     4621 tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt ggtaaaataa
     4681 aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa agttattcaa
     4741 aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa tatttattcg
     4801 aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac aaaaaaggtg
     4861 attggcacca gttcagagca agagacaacg gatatacctg ttttatattt acttcatgga
     4921 atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta tgtagcaaac
     4981 tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga tacagtttat
     5041 gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca tgaattattc
     5101 ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat gggcggatat
     5161 ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat ctctctttca
     5221 ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa agcatctttt
     5281 tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga tccgcaatac
     5341 ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat ggcttgtggg
     5401 gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca ggaacaaggc
     5461 ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg ggaccaatgg
     5521 attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct taaaaagata
     5581 gctcctatag agatcacaaa catagtgatc tctataggag cttttttact gctcgttttc
     5641 tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg ttagaaagag
     5701 tgctttttca ttcaattctg catgggactg catactttct ttaaatccct ctaattgatt
     5761 ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat ttccgtccat
     5821 aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa tcaataaatt
     5881 gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac gaacaagtaa
     5941 ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag tattgatttc
     6001 taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca tatactgcag
     6061 caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt ccattgtttc
     6121 agccattttt tgcaagagct gttgtttgtt tttgaaatac caatataaag caggtgcttg
     6181 aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt gtgtttcagc
     6241 caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt ccattttatc
     6301 aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt ctaattttac
     6361 agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat gaaattaatc
     6421 ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc cgtagcagtc
     6481 atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga agctattatt
     6541 acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct ggctttgcta
     6601 ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca aggcgtaagt
     6661 gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg aaatatcgaa
     6721 cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca aatccaaaat
     6781 ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat tggtgccttc
     6841 attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct tatcgttacg
     6901 gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat gatcattcaa
     6961 aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat tcgtgtagtg
     7021 aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag tgaggactta
     7081 acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc cttcatgctg
     7141 gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt taaagatgat
     7201 cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat tatgatggca
     7261 atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc gctgaagcgg
     7321 attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc agaacaagag
     7381 ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga ggatacagat
     7441 acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat cgtcggggcg
     7501 actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga tccgacagaa
     7561 ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag tttaagacaa
     7621 acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc tcaaaacttg
     7681 cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc tattgctcaa
     7741 gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga agaacgtagc
     7801 agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt tatcggtaat
     7861 ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc tgaaaagctg
     7921 gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat cgcacaaaaa
     7981 attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag attagtcggc
     8041 gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat ctttgaaact
     8101 caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa tttttctatc
     8161 actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg atcattgcta
     8221 cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa ttagctaatt
     8281 acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct gtaatatgga
     8341 aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt attttgttca
     8401 cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac aaattggaaa
     8461 aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt cgttttacca
     8521 gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc atcacgaatg
     8581 ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag ttagcttggg
     8641 ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc cgcaaagcac
     8701 ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat atggatgaaa
     8761 aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg attgaaggat
     8821 tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct tattctggtc
     8881 tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt atctttttcg
     8941 gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc ttagttgtga
     9001 cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca tctggataca
     9061 gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt gatgaaaaag
     9121 atgaagtcaa accagaaaac ggtaaagcat ttgatggtgt tgaaaaaggt cttgcgttgc
     9181 agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc tcgatcaatg
     9241 tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa acgacgatca
     9301 tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc gatggaatag
     9361 atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt ttgcaagaat
     9421 ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg gacgcttcag
     9481 atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc atgacgttag
     9541 aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca ggtcaaaagc
     9601 agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt ttggatgaag
     9661 caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg gatgaagcaa
     9721 tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg aatgcagata
     9781 gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcat gagttattag
     9841 cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa agttaaatta
     9901 aaagaacctc gttataacgg ggttcttttt atatatttta tgttgtttgt ttttataagg
     9961 ggca
//
LOCUS       DAZABD010000088         9727 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000088 DAZABD010000000
VERSION     DAZABD010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9727
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.090"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..129)
                     /locus_tag="ACHALG_002511"
     CDS             complement(<1..129)
                     /locus_tag="ACHALG_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFN2587845.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDS"
     gene            437..1243
                     /locus_tag="ACHALG_002512"
     CDS             437..1243
                     /locus_tag="ACHALG_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN2587846.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            1255..2475
                     /locus_tag="ACHALG_002513"
     CDS             1255..2475
                     /locus_tag="ACHALG_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN2587847.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     NKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            2465..4051
                     /locus_tag="ACHALG_002514"
     CDS             2465..4051
                     /locus_tag="ACHALG_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFN2587848.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVVQTSIAQPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSEAT
                     ERMVFRFAQQPSFIEFSAEAAGEKLIFIDSNAVVKTVNDCYLFDGKEIPEFALTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENQHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKKTSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSRDEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            4090..6228
                     /locus_tag="ACHALG_002515"
     CDS             4090..6228
                     /locus_tag="ACHALG_002515"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFN2587849.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKIRLSGKMRGKAILDFQTAKHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKTIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRQENGHISLATNSALEMAEGEFIALLDNDDELPPF
                     ALYEVAKVLNIHPELDLIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKKLGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYPNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTAMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPSYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            6516..7907
                     /locus_tag="ACHALG_002516"
     CDS             6516..7907
                     /locus_tag="ACHALG_002516"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFN2587850.1"
                     /translation="MNKNGEWNAARRIFIIIMDVLVLNVSIYLSFLLKFQGEVPARNL
                     ETFQHSAVFISIIFIALNILLGAYVFYSRVISDIVFITVIGQVLIALGIMVVTFAGRW
                     FAFPRGVIVLSFILGTILLSVYRIVVYKLYLRVSRDKKVLIVGLEKDVAPAIQNFQSK
                     KNNKHKVEAVVIDKYYENTKKLIDDIDIVYLASKIPEDEKINIYQLVTKKEKKLFLNT
                     TFENLTMINPNIMNFEDESIIEASGFKIQPEYELFKRLFDIIVSLILLILASPFMLIT
                     AILVKTTSPGPIIYKQVRITKNQKEFSIYKFRSMSVTAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNEENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLLLDLKILLQTIKILFDKMSSQGLDEDENIRS
                     LAEIDFSQEKIYR"
     gene            7966..8775
                     /locus_tag="ACHALG_002517"
     CDS             7966..8775
                     /locus_tag="ACHALG_002517"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFN2587851.1"
                     /translation="MLLSLYDKEKPEYLKEALTSIFEQTVMPKEIVLVYDGPINEKLQ
                     VVVTYFQQKDPHLFTVVKLEKNQGLGIALAIGLEHTKNRLVARMDTDDIMEPQRLEKQ
                     LEIFQKDPEIAIVGSNIEEFTGTLSNVIGRRIVPEYNDEIRVFSKRRNPFNHMTVMFD
                     KNAILEVGNYRPLIGFEDYYLWVRLLKAGYKGYNIQEILVHARAGADMYARRGGSKYL
                     LPGIKGRYRVWKDGLGSLKDFLIVICGHLVISLLPNKIRGKVYESKLRKTN"
     gene            8747..>9727
                     /locus_tag="ACHALG_002518"
     CDS             8747..>9727
                     /locus_tag="ACHALG_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFN2587852.1"
                     /translation="MNQNLEKQTKLSIIIPFYNAKNTLKRAVDSVLTQGYPNIEILLI
                     DDGSTDGSSDIGDEIANLESKVACYHLENAGPSVARNCGIKEATGNLVGFLDSDDYFL
                     PDCFNNVLKDYHRNYPDIIAFGLSKGKNIENANLFVPEKKIGCSSEEAIEQMFRSKAV
                     DFYVWNKFFKRELFNHIVFPENKLYEDMVPMYEAFRAASKIDILPFSGIFYYQNNDSI
                     VYQSFNSKQYDNILQRKILVDEVKKDYPRLINLANARLVDGYLSTGFKITGKNQNQKE
                     KQKYYNQSKREIKSIYSKIRSNNEISIAKKLALFLYLINPKTYFILYKKVL"
BASE COUNT         3356 a         1488 c         1936 g         2947 t
ORIGIN      
        1 agagtcatcc aattttcctt taataaactg cgcttgaacg cctttatatt ctttttcaat
       61 cagccattct tcatcaaaaa ataaatgttt atctgttaat ccgagtaatt ttctagtatg
      121 attatccatt gagagactcc tttggtagga tgacgttttg gtcgactaaa ttttaccaaa
      181 agtgagtctc tttttgagtt taaacgaaga aaaagtgtta gtagaaaatc gccttcgatt
      241 ttccaccaac acaaaatatt atagaacccc ttttttggtt tattgcaatt gtctagcatc
      301 ttctgtaaaa tagaaacgtt agagaaaatc ttgtatcata gaaaattaag aaagaaataa
      361 gaacgcagct cggtaccaaa aatatggtaa aatggcactt gactgtaatt cactgaaatc
      421 gcttggagga acacaaatgt ttaaagattt gtatttattt atgaaagaca tttatcagaa
      481 tagaaaatta ctgctacagt tttcgatcaa tgacttcaaa tcaagatatg caggatcgtt
      541 tttaggaatc ttatgggcgt ttatcaatcc gctatttact gtattgattt attggctggt
      601 atttggtgtt ggactgaaag cacaaatgat cgatgcaaaa tacccattta tcgtatattt
      661 agttaccggg atgattccct ggttcttctt ctcagatgca tttgcatcaa ctacggttgt
      721 ttttcgggaa tatacttatt tagtaaagaa agtcgtattc aatattcgta tcttgcccac
      781 gacgaaaatc ctatccaatc tgtacaccca tttattcttc attctgattg gcatagctgt
      841 cgcgcttata aatggtatat atccttcagc aatgtcttta cagctgattt attatttatt
      901 ttgcatgtgt gctttcttga caggattgac ttggctgacg gcatcgatcc aaccattttt
      961 acctgatatc atgcaattaa ttacgatcgt gctgcagttg atcatgtgga cattaccgat
     1021 attatggagc cctaatcagt ttgattcagg tcttgtaagc atattgaaat taaatccgct
     1081 gtactatgtt gttcaaggtt atcgtgagtc atttttaagt gaagcttggt tctgggagca
     1141 tggaacatat actttgtatt tctgggtttt cacgttagta atgttcgtaa tcggctctgc
     1201 agtgttccgt agattgaaac cacatttttc agatgtgctg taaggagaat agctatgaca
     1261 gaatatgcaa ttagactaaa agatatcaca aaatcttata atatgtacgc aaaaccttcg
     1321 gatcgatttc gtgaagccct caatcctttt aagaaatcct atcatgatgt attttatgcg
     1381 ttgaaaaacg tgaatgtaga aataaaaaaa ggtgagatga tcggtttcgt gggagaaaac
     1441 ggatcgggaa aatctacaat gttgaaaatc attacgggag tactgacacc aacatccggc
     1501 acgatggaaa tcgaaggaaa aatctcagct ttgctagaac taggatcagg attcaatcct
     1561 gagtattctg gctatgaaaa tatctattta aatggaatgg ttctaggatt ttcaagagaa
     1621 gaagtcgatg aaatggtcga tgatatcatt gaatttgctg atattggcga ccatatttat
     1681 caacctgtaa aaacctattc aagtgggatg ttcgttcgtc tagcttttgc tgtagcgatc
     1741 aatgtgaatc ctgatatttt gattgtggat gaagcattgg cagtaggaga tcttgaattc
     1801 caactgaaat gtatggaaaa atttacagaa attaagaact cgggaaaaac gattttattc
     1861 gtttctcatg atattagttc gattcgccgt ttttgtgatc gttcctattg gttgaaaaat
     1921 ggagaagttg tcgaatatgg tgatacgatg gaagtaacga cgaattacga aaactttttg
     1981 aagaaaaaat cagttaaaac agtagatcga aataaaaacc aagtagaacg cttcgcagct
     2041 ccagatatcg tagacgtgac gaaagccgaa ttgttggata aaaataaaaa tccagtggat
     2101 atgctggaac aaggagaaaa actatacgtt aaagtaacct atgaagtaaa agacgataca
     2161 atcaaaaatc cagttttagg aattgctctg cgtacagtcg acaattttta tgtgtgcgga
     2221 ctcaatactc tactagatgg tatcaagatc ccttggaaaa aaggtgagaa tatcttttat
     2281 ctggagtatg aaaagttagg tttgttagct ggtgaatatt attttgacgt tgcggtcttt
     2341 gaagaaaatg caacggttcc tttggtatac aagacaaaat acatgaactt gtttgtcagc
     2401 ggttcttata tcggagaagg catcgtggtc ttagaccata aatgggaaga agggactcat
     2461 agcaatgaaa tatgattttg aaatggacct agatgaacaa agcagtgtgg gaaaaattgc
     2521 cgcacagatc aaaccaggaa gtaaagtttt agagtttggt cccggaaacg gtcgattaac
     2581 gaaacatttg atcggagcaa aacaatgtga agtgagcatt gttgagctag ataaggaatt
     2641 attcgatttt gtcagcgaat ttgcccaaga tggattttat ggagatatcg aaagttttga
     2701 atgggcaaac tattatgctg gacagacatt tgattacgtc ttgtttgcgg atgttttaga
     2761 acatttagta gatcctggaa aaacattgaa gaaagtcaga gaatttttga atgaagaagg
     2821 agagatcctg attacattcc ctaacttagc acataattct gtcatgatcg atttgttcaa
     2881 taatcaattg ccttgggctt cttatggtct cctagatgaa acccataatt ctttttatac
     2941 tcatgatggt ttccaaaaag tttttgaaaa agccggatta ttcattaata ttgaagatta
     3001 cttgtacttg gctgtaggag atacagaatt aaaatcaact tatgaagagt tgccggaagc
     3061 tgtacgctat gatttcaaaa tgcgaccatt tggtgaagtc taccaatact ttttctcatt
     3121 gatgaaacat ccagtagtac aaacttccat tgcacagcca caaaactcta attatgtaaa
     3181 agtactagaa gtaacccagc aaacgaaaca agatgaaaca atccaacaaa taccattcaa
     3241 taatttcaca ggggaaaatg agacactaag tttccccgtg tcagaagcaa cagaaagaat
     3301 ggtgtttcgc ttcgcacagc aacctagctt tatcgaattc agtgcagaag ctgctggaga
     3361 aaaactcata tttatcgatt caaatgctgt agtcaaaaca gtaaatgatt gttatttgtt
     3421 tgatgggaaa gaaataccag agttcgctct aacggatatt tctgggaaag aagtgactat
     3481 ccattgtcac tatcgcttta tcggggaatt gacaccaaca atgaaagaat tgctggaaac
     3541 aattcgtcct atggctgaag tcacaaagca gctttctgag aaaaatgatg aattagaaag
     3601 agaaaatcag catcttcttg aggaaaatac aagactagac catacgttaa aaacaaccac
     3661 aaatcgctat tgcacattgt tggaaagtga tgaatggacg atcaaacata gactaggacg
     3721 acgtaagaaa aaaacatcga aaaaaataca agaaaaagag ctttctatat gtattgatga
     3781 gaaaatatgg gatgcagaaa caaaaatatt aaaaatcatc ggatggggaa tcgctaattc
     3841 ggatcgccag ccattgtcct ataagttatc agctgaccaa tcgccatttt tcgaagcaat
     3901 tcctgtttca cgagatgaag tcaatcaagc agaacagctc gcaaaaggaa cagaagcggg
     3961 atttgaatta cgaatccttt gcgagcaaga acgttcattc ttagtggaag cagtagctca
     4021 aaatggtcaa tcatggatca ttgaaatgta aataatgatt aacttataaa gataaggagt
     4081 aaacgaatcg tgcaaaaaaa agaaattgcc gtatttatcg atcaaatcac tcgtgaacgg
     4141 gccactggcg atttgcttat tgtgggctgg gcaattgatg aagtgaccaa agaaatccct
     4201 acgataaagg tagaaaaaga aaatgtgatt gctgaagcaa cacatgtcgt aagattggat
     4261 atcaatcatt tatataatct tgatgttaag acccaaagcg gttttaagat acgtttatcc
     4321 gggaaaatga gaggaaaagc gatcttagat tttcaaacgg caaaacatca aaatggcata
     4381 gctgtgaaac taaatggtaa atacccttat gatgacggca ttgaatcttc atgggaaaga
     4441 aaaaaaagat tactgaaaaa agggataaat tatgcaagga ctcatggcgt aaaaaaaaca
     4501 atccgccggg tcaaactgga attgaaacca ggatcgatcg actatgcaga gtggatcagt
     4561 cgtcacgaga aacctgatct gaaagagcaa aggaaagaag cacaagggtt tgcataccgt
     4621 ccattgattt ccatacttat gcctgtctat aacgttgaaa tcaaatggct cgaaaaatgc
     4681 attgattcgg tattggatca aacgtatgat cactgggaac tatgtatcag tgatgatgct
     4741 tctactgatc cagctatccg aaagtgtctg gaatcctatc aagcaaagga tgatagaatc
     4801 aaagttgttt tccgacaaga aaatggacat atcagtctgg caactaattc tgcgttggaa
     4861 atggcagaag gagaatttat agcgctctta gataatgatg atgaattacc gccatttgca
     4921 ttatatgaag tggcaaaagt gctaaacata catccagaac tggatttgat ctacagtgac
     4981 gaagataaaa tcgatgcaga tggcaatcgc tttgatccgc acttcaaagc agactggtct
     5041 cccgatacat tgatgggaaa taattatatc tctcacttag gcgtatatcg aacaagtatt
     5101 gtgaaaaagc ttggcggttt ccgtaaaggc tacgaaggct ctcaagatta tgatttggtc
     5161 cttcgtgtga cagaacaaat ccctgcagat catatttacc atattgaccg agtcttgtat
     5221 cattggcgga cgatcccagg atcaaccgca agtaatggtg aagccaaaag ttatatctat
     5281 gattctggag tgaaagcatt gacagatgcc ctgtctagaa ggaatatcaa aggaagcgta
     5341 cgtcctggca gaatttctgg attttatgag attgcttatg acgtcttgca agaggatttg
     5401 gtcagcgtga tcattccaac gaagaatggc tatgaagatc taaaaacttg tgtggattcg
     5461 attatcgaaa aaacgtctta tccaaattat gaaatcatta ttgcagataa tggaagtaca
     5521 gatcctaaaa tgcaggaatt atttgcagaa tacaagcatc agctgaaaga ccgatttatc
     5581 gttgaattaa ttgacattcc attcaattat tcacgtatca acaatctggc agcagaaaaa
     5641 gcaaacggca aatacttttt attcttgaat aacgatacag aagtgattga acctgactgg
     5701 atgacagcga tggtttctta tgcacagttt gatcgaatcg gctgtgtagg agcaaaactg
     5761 ttttatccag atgatacaac gcagcatgca ggagtattat taggaatcgg tggtgttgca
     5821 ggacatgcat tgaataacta tgatcgaaca cattgtggtt atttcggccg attggtcatt
     5881 gacgtcaatt acttagcagt cactgcagct tgtatgatgg tcaaggcagc tgacttcaat
     5941 gctgtcaatg gatttgatga aacattagaa gtagcattca atgatgttga cttgtgtttg
     6001 aaagtttatg aactgggtcg ttacaatgtc tatgcacatc aagcagaatt gtatcatttc
     6061 gaatctaaat caagaggtta tgaagataca ccagaaaaac aaagacgttt tgccggggaa
     6121 ataaaaaaaa tgcaagataa gtggccttct tatattgctc atgacccatt ttataacgat
     6181 aatctaacta aagaaggaat cggcgatttt tcattgagac ctgattgatt aaaagaactg
     6241 tattttcatt tttattgatg aaaatacagt ttttttagta tatgaaaatt gtaacttgtg
     6301 accgaagaga tgataggaag aacacccatt cagtaggaag aatgtgatac gatttttgtc
     6361 caactctctg ttaggcagaa atatacatat aaacatacaa tagtcacctt ttctagtttc
     6421 ttttaattta ttttacaatt tggttattca ctaggttttt aaactttgct atggtaagat
     6481 gatagaggca ttattttatg gttaggagtg tatatatgaa taaaaatggg gagtggaatg
     6541 cggcacgtag gatattcatt atcatcatgg atgtacttgt attgaatgtg tcgatctatc
     6601 ttagcttttt gctgaagttc caaggagaag tcccagcaag aaacttagag acctttcagc
     6661 attcagcagt ttttatttcc attatcttta ttgcgttgaa tattttgtta ggagcttatg
     6721 tattctatag ccgagtgatc agtgatatcg tcttcattac tgttattgga caggtgctga
     6781 tagcattggg gatcatggta gttacttttg ctggacgttg gtttgcattt ccgcgtggcg
     6841 tcatcgtgct ctcgtttata cttggcacga tattacttag cgtttatcgg atagttgttt
     6901 ataaactata cttaagagtc agtcgtgata agaaagtcct tatcgtaggt ttggagaaag
     6961 atgtcgcacc agctatccaa aactttcaat ctaagaaaaa caacaaacat aaagttgaag
     7021 cagtagtgat cgacaagtat tacgaaaaca caaaaaaatt gattgatgat atcgatattg
     7081 tctacttggc ttctaaaatc ccagaagacg aaaaaatcaa tatttatcaa ttagtaacta
     7141 aaaaagagaa gaagctattt ttaaatacaa cttttgaaaa tttaacgatg atcaatccga
     7201 acatcatgaa tttcgaagat gaaagtatca ttgaagcttc cggtttcaaa attcagccgg
     7261 aatatgaatt attcaaacgt ctttttgata tcatcgtttc gcttatttta ctgattcttg
     7321 cttcaccttt tatgctaatc acagcgattt tggtaaaaac aacttcacct ggaccaatta
     7381 tttataaaca agtacgtatt acgaaaaatc aaaaggaatt ttcaatctat aaatttcgtt
     7441 ccatgagtgt aacagctgaa gcaaaatcag gaccggtctt ggcaaagagc aatgatgcac
     7501 gtgtgacacc agtaggcaaa tttatccgtg ccgtacgttt tgacgaatta cctcaaatct
     7561 ttaatgtatt gaaaggcgat atgtcgatcg tcggaccacg tccagaaaga ccatttttcg
     7621 ttgatcagtt caatgaagaa aatccttatt attatttaag acacaatgta cgtgcaggga
     7681 ttaccggata tgcacaagta tacggaaaat atgtatcaga ttataatagt aaactacgtt
     7741 ttgatttgtt gtatatcaaa aagtattcct tactattaga tttgaagatt ttacttcaaa
     7801 caatcaaaat cttatttgat aagatgtcct cacaaggact agatgaagat gaaaatatcc
     7861 gttcgttagc agaaatcgac ttttcacaag agaaaattta tcgctagatg ggaaaaattt
     7921 tgagcaaaga agtaggagag aacattggaa aataaaaata taagtatgct gctttcttta
     7981 tatgataaag aaaaaccaga gtatttaaag gaagcattaa ctagtatttt tgagcaaaca
     8041 gtaatgccaa aagaaattgt attagtttat gacggcccta taaatgaaaa acttcaagta
     8101 gttgtcactt atttccaaca aaaagatcct cacttgttta cagttgtaaa attagaaaaa
     8161 aatcaaggat tggggattgc tttagcaatt ggattagaac atacgaaaaa tcgcttagta
     8221 gccagaatgg atacagatga tatcatggaa ccccaacgat tagaaaaaca attagagatc
     8281 ttccaaaaag atccagaaat agcgattgtt gggagtaata ttgaggaatt tactggcacg
     8341 cttagcaatg ttatcggaag aaggattgta cctgaatata atgatgaaat ccgtgtgttt
     8401 tcgaaaagaa gaaatccctt taatcatatg acagtcatgt ttgataaaaa tgctatttta
     8461 gaagtgggaa actatcgccc attaattgga tttgaagatt attatttgtg ggtaagactg
     8521 ttgaaggcgg gatataaagg gtataatatc caagagattc tagtccatgc tagagcaggt
     8581 gctgacatgt atgctagacg tggaggaagt aaatatcttt taccaggaat taaaggccga
     8641 tatcgcgtat ggaaagatgg tctaggatca ctaaaagatt ttctgatagt tatttgcgga
     8701 catcttgtaa ttagtttatt accgaataaa attaggggaa aagtatatga atcaaaactt
     8761 agaaaaacaa actaaacttt ctataatcat acctttttac aatgcaaaaa atactttaaa
     8821 aagagctgta gacagcgtct taacgcaagg ttatcccaat attgaaatct tgttaattga
     8881 tgatggatct acagatggta gtagtgacat tggtgacgaa attgctaatc tagagagtaa
     8941 agtagcatgt tatcacttgg aaaacgctgg tccatcagtt gctaggaatt gtgggataaa
     9001 agaagcaact ggaaatttgg taggattttt agatagtgac gattattttt tacctgattg
     9061 ttttaataat gttttgaaag actaccatag gaattacccc gatatcattg cttttggatt
     9121 aagtaaagga aaaaatatag aaaatgcaaa tttatttgtt cccgaaaaga agattggttg
     9181 ttcaagtgaa gaagcaattg agcaaatgtt tagaagtaag gcagtggatt tttacgtatg
     9241 gaataaattt tttaaaaggg aattattcaa tcatatagtt tttccagaaa ataaattata
     9301 tgaggatatg gttcctatgt atgaagcttt tagagcggct agtaaaattg atattttgcc
     9361 attttcaggt attttttatt atcaaaataa tgatagcatc gtttatcagt cttttaattc
     9421 aaaacagtat gataatattt tgcaaagaaa aatattagtt gatgaagtaa agaaagatta
     9481 tcctcgactt attaatttgg ccaatgctcg attagtagac ggataccttt caacagggtt
     9541 taaaattact ggcaagaatc aaaatcagaa agaaaagcaa aaatattata atcagtctaa
     9601 aagagaaata aaaagtatat attcaaaaat aagaagtaac aatgagatat caattgctaa
     9661 aaaattagct ttatttttat acttaattaa tcctaaaact tattttattt tatataaaaa
     9721 agttcta
//
LOCUS       DAZABD010000089         9627 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000089 DAZABD010000000
VERSION     DAZABD010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9627
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.091"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..258
                     /locus_tag="ACHALG_002519"
     CDS             <1..258
                     /locus_tag="ACHALG_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587853.1"
                     /translation="LKRKVTDRNKNLLNVVMICYISFLILGLPQAVFPEMFSGDWGIV
                     SYSILTLIAMVLGTSFYNAFVSKHVEEYEVLMYLKEKKTSN"
     gene            465..665
                     /locus_tag="ACHALG_002520"
                     /pseudo
     CDS             465..665
                     /locus_tag="ACHALG_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298761.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1377..1982)
                     /locus_tag="ACHALG_002521"
     CDS             complement(1377..1982)
                     /locus_tag="ACHALG_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFN2587854.1"
                     /translation="MAKIGYIRVSSQDQNLDRQLAMMEDLNIDKLFQEKASGKDIQRP
                     EFQKLLNYIREGDCVIVASLDRLGRDYEDIKNTVTFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAKKAGRYKGRPIEYSENSTDPQ
                     KRLVFKTVVDMLNQGVPVAEIAKENGLSRPTIYKIKKANDL"
     gene            2164..2499
                     /locus_tag="ACHALG_002522"
                     /pseudo
     CDS             2164..2499
                     /locus_tag="ACHALG_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387932.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            2664..2879
                     /locus_tag="ACHALG_002523"
                     /pseudo
     CDS             2664..2879
                     /locus_tag="ACHALG_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397514.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3017..3988
                     /locus_tag="ACHALG_002524"
     CDS             3017..3988
                     /locus_tag="ACHALG_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN2587855.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICEENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            4128..5441
                     /locus_tag="ACHALG_002525"
     CDS             4128..5441
                     /locus_tag="ACHALG_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HFN2587856.1"
                     /translation="MKKRMLAIAAILALTAGGLAGCGSKEKSSGSSDPGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAY
                     TPFIWSNGGDVVNADGTKAEDYFNGKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMMLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASA
                     AFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQLSGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            5484..6350
                     /locus_tag="ACHALG_002526"
     CDS             5484..6350
                     /locus_tag="ACHALG_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN2587857.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLTPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQIGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            6347..7195
                     /locus_tag="ACHALG_002527"
     CDS             6347..7195
                     /locus_tag="ACHALG_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN2587858.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            7209..8681
                     /locus_tag="ACHALG_002528"
     CDS             7209..8681
                     /locus_tag="ACHALG_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HFN2587859.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWMNDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMIAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQERIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPDHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            8698..9570
                     /gene="scrK"
                     /locus_tag="ACHALG_002529"
     CDS             8698..9570
                     /gene="scrK"
                     /locus_tag="ACHALG_002529"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFN2587860.1"
                     /translation="MIYGSIEAGGTKFVCAISDEEMTIKERVSFPTTTPEETIPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKVAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT         3023 a         1652 c         2006 g         2946 t
ORIGIN      
        1 ttaaaaagaa aagtgacaga taggaataaa aacttattaa atgtagttat gatctgttat
       61 atttcatttc taatactagg tcttccacaa gcagtgtttc ctgaaatgtt ttctggagac
      121 tggggtattg tcagctattc tatattaaca ttgattgcta tggttttagg tacttcattt
      181 tataatgctt ttgtaagtaa gcatgttgag gaatatgaag tattaatgta cttaaaagag
      241 aaaaaaacaa gtaattaata tgagaacacc acgcatagtt tttattttga gatattctag
      301 aatgaaacac attaagaacg aaagcaaagc aaaagacgcc agaacttcta ctaccttcta
      361 agttttttca tatttgtttg ttatgctata catagatctg cataattaga cttgcgctgc
      421 ccgttattga agtttcaggc agcgctcttt ttgggggatc gataatggaa gaagaaaaaa
      481 tatttgagaa acgctggcag ctagcatcaa gtgaacaaag agcaagatac aataatttga
      541 tcacctccta ccccactgtt gactagacat ataaggaaaa gaaatactta ctttggctgt
      601 gccagttaga tatagatacc tttgaaacat ttgaagtgat cctagataaa attaaacaga
      661 attgatcaga aacaagcgaa gccttctctc ggttcgcttg ttttgctatt tattagttgg
      721 ctcagggcaa ctctaagctc gtttgaaaga aaaccgatgg ataatcccct tgtttgtacc
      781 actttagaat acatggatgt acgtattgcc attgactcct attagttact gaaaaaaaat
      841 ttaaacaagt tgagtattgc agataaatag ctccagtgag gtgaataact gctccactca
      901 aggcaataga tagctccatc cagggcaaca aactgctcca ctttcatatt taatagatga
      961 ataaccttct gtataataga aaggcatacc tttcggtatg acaacttata cagaatgtta
     1021 ttttattggt tcactatcga aaaattttgg agctttattt ttccggtatt tctcaacgaa
     1081 cgattagttt aactgtggga agctccagac atacgattcg agaagttact tctcgagcag
     1141 acaggaaggg ggtgactgaa ctaaccgctg atatgagcga tacttggctg taggaatatc
     1201 tttttccgga aaaacaaaca acagcacgag gttaccttcc tgtagactgg gaatatgttc
     1261 ataaagaact aatgaaaaag aatatgacat tgaaactcct tcatagagaa tatggggtac
     1321 atggaacata gaaacgaaaa tgcacggtag aaaagcgaat gaatatagta taaattctac
     1381 aaatcattcg ctttctttat tttatatatg gttggtcgag agagaccatt ttctttggca
     1441 atttctgcaa ctggaactcc ttgatttaac atgtccacca cagttttaaa gactaatcgt
     1501 ttttgcggat ctgttgagtt ctcagaatat tcaattggtc gccccttata tcgaccagct
     1561 tttttggcca ataatactcc ttgccgctga cgttcacgga ttttctcacg ttcgttttgt
     1621 gcaatataac ttagcaatga taaaaacata tcgaacatag cagtatctag gagttcattt
     1681 ccagtattaa aatctaaaaa ctgtgcatct agtattttta atgctacttt tttctgtttc
     1741 ataaaggtaa ctgtattttt tatgtcttca taatctctgc ctaggcgatc cagtgatgca
     1801 acaatgacac aatcaccttc tcggatataa tttagtaatt tttgaaactc aggtcgctgg
     1861 atatctttac cactggcttt ttcttggaat aatttatcaa tgttaaggtc ttccatcatt
     1921 gccaattgtc gatctaaatt ttgatcttgt gaagacactc taatatatcc aatttttgcc
     1981 ataaaaactc cttttaaaaa atgatataat ttacatgtaa aatttacttg tagttttact
     2041 gttatcatag catttttaaa aataaatttt aagtctactt ataatttact ttggaggttt
     2101 tgaatgaatg aaattaagcg caataaaaat tcttttggtg tttatttagg agagcctagc
     2161 tcggaacagt taaataaagg tgaagctcgt catgctctat gtcgaaatat attttatggt
     2221 agaaaaggca aactttatca aacttattac gacggtatgg aggaacagct caacgcatta
     2281 ggattagtaa ctaatgctat catatattgg aattcactat atttagaaaa agtctttgaa
     2341 caaattaaaa cggaagggtt cgactgttcc gaagaagtgt tacgcaagca atttccatta
     2401 attagtgatc acattaattt tgttggcaaa tatagtttcc gctatgaaaa agaattatcg
     2461 aatggaatac tacgtccgtt aaatatagag aattattgag aatgttcgtc ttttggttcc
     2521 taccgtgcat tttcgcacgg atgttccaga tactattttt gaatgtataa aattgaaaac
     2581 acaagaatac taataaatca acgattcttg tgtttttaat ttttcactac cgtgcatttt
     2641 cgtttctatg ttccatgtac cccaatcttg aaaaattaat taaagactta gataaagcca
     2701 ttgacgactt agtcgtggtg ctaccggagt atcaatgttt aaccagtatc ccaggtattg
     2761 gtcctgttta ggctgctgga atactcgctg aaattggtcc aattgaacgt ttttctgatg
     2821 aaataaaatt gacaaagtat gccggcctat attggcgaca gagtcaatcc ggtaaatctg
     2881 aatacgaaaa tttttttacg aaaagcttca cgtctaatat ctattttaaa ataaaaatat
     2941 tcctgctaat ttggaatcgt ttccttcttt tggtataata atacctgaaa ttcttttgag
     3001 aggagttgtt cgtgaagtga aaactactat gaaggatgtt gctgcgttag caggagttgg
     3061 tgtgggtacg gtatctagag taataaatgg agtaaaggtg aaagaggtta ccaaagaaaa
     3121 agtaatggct gcaattagtg agcttaatta tgagcctgat gaatatgcca gaggtatgaa
     3181 aacgaatagt agcaatacaa tagctcttat tttacctacg atttggcatc ccttcttttc
     3241 cgagtttgcc tactatgtag aggaagctct cagccaaaaa aattataagc tttttttgtg
     3301 taatgcgaac ggcagtgcgg aaaaagaaaa agaatatatc caaatggtca agcagagtaa
     3361 agtagacgga attataggta ttacctattc agacattgat aaatatgtat catcaaattt
     3421 accatttgtc agtatagacc gccatttttc ggaacaggtt tcttatgtaa cggcagataa
     3481 ttatttaggt gggcaaattg ccgctcaaga actacttact cgtaactgta aaaacttgtg
     3541 ctatgttggg ggtgtttcac cttaccctaa cgaaacaaac aatcgtaaag ctggttttta
     3601 caatatttgt gaggaaaata aaatagctgt gaaggtattg gatatgcctg aaccaattag
     3661 caatttagag gaacaattgc tcgctttttt ttcggataat cctgaaattg atagcatttt
     3721 taccatcaat gatttcatgg ctttggatgt gataaaagcc caaaaaaaac tagggaagga
     3781 agcacctaag gattttcaaa ttattggttt tgatggagtc aagctatcgt tgcaaaaaga
     3841 ctatttacta tcaacgattg tacagccgat agaggaaatg gccaaggttt cagtagaaac
     3901 actcatggcg atgattttaa atgaagaacc gcctaagcgt gtggtagtac cggttttctt
     3961 tcacgctgga gagacgacta aagagtaaaa aaccggttta taacgatttt tttgagatag
     4021 gttgacagaa agtgatttca atgatatctt aaaaccaatt aattggaaac gttaccaatt
     4081 aattgaaaaa taaaacggaa acgtttcaaa aaataggagg aacatcgatg aaaaagagaa
     4141 tgttagcaat tgcagcaatt ttagcattaa cagctggagg acttgcagga tgtggcagta
     4201 aggagaaaag ttcaggtagc agtgatccag gagaaattaa gatttgggtt cagttttctg
     4261 atgaaacgcc tgaaggaaaa gcttgggcag agattgtgaa caattttaat gatgaatacg
     4321 agggtgaata taaagctgtt actgagtata ttcctcgtag cggaagcggc ggtggatatg
     4381 aggataaagt aaatgctgca attacaacca atacccttcc tgatgttatt acgttagatg
     4441 gaccgaatac tgcagcttat gctcattcag gagtcattgc tccattagat gattatttga
     4501 aagatgcgga catggatgat gtattagaca gcattattca gcaaggaacc tacgatggca
     4561 aaatgtatgc gtttggtttc tcagagtcca gtgtgggtgt ctattacaat aagtcgctgt
     4621 tcaaagatgc tggaattaca gaagatcaat tgccgacgtt agaagatcct tggacttgga
     4681 cggaatttca agcaatttgc caaaagctga ctgataaata caaaacgcct gcaattgata
     4741 tgcaaatggc gtcgggagac gaaatgctga cctacgcata tacaccattt atatggtcga
     4801 atggcggcga tgtcgttaat gctgatggaa ccaaagccga agactatttt aacggtaaaa
     4861 aatccgttga agcgttggaa tttattcaag aattggttga aaaaggttac accacaatca
     4921 gtcctattga aaaaggattt gaaactggca aatatgccat gatgctgagc ggttcttgga
     4981 caatggcaga tttagaggaa aactatcaag agattgatta tggtattttg ccttaccccg
     5041 tttcagatgc aactggggag ttagtttcac caaccggaag ctggcagtta gcaatgactg
     5101 agaaaacaga gaaaaaggat gcttctgcgg cctttattaa gtttgcaacc agtacagaat
     5161 ctagtcgtat tatgagttta gggaacagtg ttctacctat cagaaattca acagttgatt
     5221 taattaaaga tgaagtgtcg gaaggaatgc aggtattaat taagcaaaac caactttctg
     5281 gacatgcccg cccagtggtt gtagcttatc cgcaagtatc tagagcattc caacaagcag
     5341 ttcaagatat tagctacttc gaggaaaacc cagatgtaca aaaagtagca gatagtcgcg
     5401 ctgctgaaat gcaacaggca attgatactt cactaaaata gaagtttcta aagggggcca
     5461 atcaagtgtt aaaaaaaccg acaatgaaag acaatttgat aggttacggc tttctagcgc
     5521 cagctttgct gctgctgacg atttttctga tcatacccgt aatcatggta atttattatg
     5581 cctttactga ttattatttg ttaacgccag atgctagaca atttatcggc ttagaaaatt
     5641 ttaagcgact gttcgctgat ccaatttttg taaaaagtat ttggaacacg gctaaatttg
     5701 ttgtttggat tattccggtc caaatagggg cagctttagg aatggcactt attatcaaca
     5761 aaaatcgtaa aggaaatatc ttttttaaag tagccttttt tgcgccagtc gtgatgtcct
     5821 tagtcgttat ttcgatttta tggttgtact tgttaaatcc taatgaaggt ctggttaata
     5881 cgctatttgc aaagattgga attgcttcgc agcctttttt gactagtccc aaacaagcaa
     5941 tgtatacgct tgttttcgta tcggcatggc aaggtgcagg atatcagatg ctgctgtttt
     6001 tagggggaat gcaaaatatt ccgcaagacg tttatgaggc agccgaaatt gatggttttt
     6061 ctaagtttca gcaatttcgc tatatcacca tgccattatt aaagcccacg gcgattttta
     6121 ttttacttac cacattgatt tcggccttta aactaatcat tcagccaatg gtaatgactc
     6181 aaggtggacc aatgaattct actatgacaa tggtttatta catttaccaa actggattta
     6241 cagaccgtat ggtaggctac tccagctcca ttgcgttaat ctttaccact atgattggtt
     6301 taattacaat tgctcaaaga aaattggtaa aggaggatga tccagcatga ccaagaaatt
     6361 tggaccgcta atgatcgtag aatattgttt gctgttcctt ttagcggcat tattcatttt
     6421 cccgatgctg tggatggtag tttcctctat gaagcctgaa gcagatgtct atcaaaattt
     6481 agcttccttc aaagcttttt tacctagttt gaatccagct aattggttta aaacttatca
     6541 agaggttatt tcccggttca gtgtgggaac ttatttatta aacagtattt tttatggttt
     6601 aacctttgcc tgtgggtcaa tcattgtaaa ctctttggcg ggttttgcct ttgcgaaggt
     6661 taatttttca ggcaagaaaa ttatctttgg agctttgtta gctctgctga ttgtcccgat
     6721 ggagacggtt ttaattccgc aatttaccat cgttaattca ctaggcttag tgaatactcg
     6781 cttggcagtg attttaccag cgatggccag cgcgtttaat atctatctgt ttagaaattt
     6841 ctttatcgcg attccagaag aaatcattga atcagcgaaa ttagatggtg caaatattgt
     6901 taaaattttc tttcacatca tgctgccaat gtctaaacca gccatagcca cagtaggtgt
     6961 cttatccttt atctccagct ggaatgatta tatttggccg ttaatggtct taactgacag
     7021 cagcaaattt tcaatgcaag tggcaattac tacgatcaat actacacagc cagtttatat
     7081 caatcaagtg atggctgttt taaccattgc aacgattcca ctgattattg tttatatcgt
     7141 tgcacaaaaa tatattctgc agggacttgg cggttctgga acgggtataa aataggagtg
     7201 aatagtgaat gttgcaaaaa cttttaaagg ccaatgagtt tattgagaaa aatgctcaaa
     7261 cagtcaatca agaataccgg aataaatttc atttgatggc tccgattggc tggatgaatg
     7321 atccaaatgg ttttgtttat tatcaagagg agtatcactt gttttatcaa tattatcctt
     7381 acgacagcat ctggggacca atgcattggg gccatgctaa aagcaaggat ctaattcact
     7441 gggagcattt gccagtagca ctggcaccag gtgaaagcta tgacaaggaa ggctgttttt
     7501 ccggcagtgc catagaaaag gatggcaagc tgtatttgat ttatacagga cacaatgttg
     7561 tggaagggca agttcgacaa gttcagtgtt tggcagtttc agaagatggc attcactttg
     7621 aaaaatatgc cggaaatcca atcatcgcag aagatcactt agcttctgtt gcaacgaccg
     7681 aagatttccg cgatcccaaa gtgtttcagc gaaaagaaga ctattttact gtagtcgcta
     7741 ctaaaactgc agatgctcgt ggacaaattg tgatgttcca ttccaaagat ttactaactt
     7801 gggagtttta ttccgtattg ctggagggaa ctccagaaca aggcattgta tgggaatgcc
     7861 cagacttgtt tgaactagat ggcaaagaag tcttgattct ttctcctatt caaatgactc
     7921 cacaaggcaa tgcttaccaa aatacgaact caactgttgc tttcatcggt actgtcgatt
     7981 ggcaaacagg caaatttcta gtagagaatt ttcatgaaat cgatggcggt ttagattttt
     8041 atgcacccca gacctgtgaa aacgaagcag gccaaagaat aatgattgct tggatgcaaa
     8101 tgtggcagcg taaaatacct acgaatgatc tgggccatgg ttggtcaggt tccatgacct
     8161 tgcctagaga gctgagggtg gctgataatc aattgttgca acggccagtt tcatcaattt
     8221 atcaagtaat tgatccgcag gaaaggattg aatgggcgaa gactgaagag aatcgcttct
     8281 tactgaagga cttagtatat gaccagcact atttacgctt ggaaatcaat ttaaaacagg
     8341 caacgaaact agaacttcac tatgcgaagg gacccgacca tacattactg cttaactatg
     8401 atgttgaacg ggagtgcttt actgcttcca gggaacacat gccgtatgat ttatcaggag
     8461 ctgaaaagga agtgttgaaa aagcgcagtg tgacgattcc tttggaaaat aattgtctaa
     8521 ccatcgaaat tttccgtgat acttcagcta ttgagctttt tctaaatgat cgtctgacta
     8581 tgagtacaac tttttatgag acagaaaaag gacaggattt tcttttgctg gcagacagtg
     8641 aagtaacctt ttcattggaa agcggcttaa ttaatctata gaagggagag ctcagtgatg
     8701 atttatggtt caatcgaagc aggcggcaca aaatttgtgt gtgccattag cgatgaagaa
     8761 atgacgatca aggaaagagt gagctttccg acaacgacac ctgaggaaac aataccttta
     8821 gtcattgact tcttcaaaca ataccaagca gatttagcag ggattgggat tggctcattt
     8881 gggcctatcg atatccatcg cgattccgcc acttatgggt acattacctc gaccccgaaa
     8941 gtggcatggc aaaattttga tttcatcggt accatgaaaa aagaatttcc gatcccgatt
     9001 tcttggacaa cagatgtcaa tgcggctgcc tatggtgagt atgtcttcgg cagtggcaaa
     9061 ggattgagca gtgtcgttta ctatacgatc ggtacgggtg ttggcggcgg tgccttacaa
     9121 gaagggcgct ttatcgaagg gtttagtcat ccagaaatgg ggcatatgct cgtcgtccct
     9181 catccaaaag atgactttgc tggcagctgt ccatttcatg gcaactgttt agaagggatg
     9241 gccgctggac ccgccatcga aaaacgctta gggaaaaaag gacaagaggt tgcagaagat
     9301 gatccttatt gggaaatcga agcctcctat attgctcaat gtgcctacaa tacaaccttg
     9361 atgttttctc cagatgtgat catttttggc ggtggtgtga tgaaacaacg ccatatgctg
     9421 caaaacgtcc acgacgcatt tgcaaaattg gtcaatgggt atgtcgaaac acctgagtta
     9481 agcaaatata tcgtgacccc agcattagaa gacaacgctg gaacgctcgg ttgtttggca
     9541 ttggctcgag aagcggtctt acattcataa acgaaaaaag gctcttcgac atttagtgtt
     9601 ggtagagaaa gatacaactg aaaattt
//
LOCUS       DAZABD010000090         9133 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000090 DAZABD010000000
VERSION     DAZABD010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9133
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.092"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(360..1808)
                     /locus_tag="ACHALG_002530"
     CDS             complement(360..1808)
                     /locus_tag="ACHALG_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016620271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN2587861.1"
                     /translation="MYKEDLLDKDLRLEYTLLKFLYLSTGHIKKSEACQELNITMPTL
                     IKLSDNLKRQLKNEKISFIINRYTLELKVNDSVSLDDLTDILLKKSIKYQIMEYLFHY
                     KKYDAIVLTDKLKISIGTLNRLISECNRSLQHLELKIRNKKLSGSLTQRIYFFYNFFK
                     MGSKKIDSELIDNIIAKLIKKITLSIAQQKQLKIWIYVLMKNISNKAILKNLNPEEQR
                     SISRYQNKSIFQYVQHTYCSQKKICSDDEAKIVTYFICLFLITFGGVPYEEIRLGLYS
                     RNNPVFEITDEVMAAIQSTYNLIKEHSSIDIKSGVFSLIAQIYYFDGVNYSIDKVTLN
                     YYYGLFNNSFRNQFVLDILKYIIEPTNLFNPSKMNYLKKRLIFLIYMLSPKKTYKVRI
                     GVLNMESHLLSDAIIYLLKNELKGKQNVTVLSYTQNEPFDLLISNANLPQLGKNYDQF
                     YQVTVIGADLDLNQVSSIVDQIAYSKYRSFVV"
     gene            complement(1985..2944)
                     /locus_tag="ACHALG_002531"
     CDS             complement(1985..2944)
                     /locus_tag="ACHALG_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFN2587862.1"
                     /translation="MTYNHLTPTELVMIEAYFNQSQPVSKVANLLQRSRQTIYKVYRF
                     LKSGGSAMSYYKQYKKNKRNCGRHPIVLPDEQQEYIQKKVVQGWAPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDTHDLPMKGKRKPNGHKEKRGKQGFRRSIRDRKTDYTQFDHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPEGRQAKDIENRLNQWFRCVPKNLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNG
                     VSETFIQSVASKRNHIPRKSLHYRTPMEVFLSYVTKDDLSNLI"
     gene            3337..3717
                     /locus_tag="ACHALG_002532"
     CDS             3337..3717
                     /locus_tag="ACHALG_002532"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587863.1"
                     /translation="MEERSLSTSTLCIHLYTKAGKLLAVCYNVLKYLESGIYNIHYSA
                     VPIDGNTSGGITPTEKIARKTVRLIVLPNGGLVGKTGAALSSVVLPEGWSWKNANEII
                     AADSSYSLLYTNAKDDTVDETKTI"
     gene            3698..4495
                     /locus_tag="ACHALG_002533"
     CDS             3698..4495
                     /locus_tag="ACHALG_002533"
                     /inference="COORDINATES: protein motif:HMM:NF014967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HFN2587864.1"
                     /translation="MKRRLYEITIPSMYITEEPINIDQSDRNILVLDNMILDGEKATI
                     DLISNKNNDKIDTIMSMRTGSFTFRYSLDSESTITNSELTTLFSISYGAAESNYFSFY
                     IRPKNGKIGIEVRRNGKSYLKEVGTGFNLLNNADWHFITYTFNGVYMQVYLDGILHGE
                     TSFTGLFYNNSWAEHADRLSVGGTVRKNNVQWRLKGLLDTVIVTRDVLSPDEIKTLHR
                     VTDNEAIGEKSSMWDKYDEDIFEYRIPSIVKLPDGTLVAAADARKRH"
     gene            4580..4831
                     /locus_tag="ACHALG_002534"
     CDS             4580..4831
                     /locus_tag="ACHALG_002534"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587865.1"
                     /translation="MPTQPYFTSEYLTTDWNTKHTQSAFSIDPILLADSSGKLYLLVD
                     VFPESRGSIHSKPGDGYTLVDGKYYLTLNDKSKKTIYCS"
     gene            4973..6502
                     /locus_tag="ACHALG_002535"
     CDS             4973..6502
                     /locus_tag="ACHALG_002535"
                     /inference="COORDINATES: protein motif:HMM:NF024488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HFN2587866.1"
                     /translation="MRSKNDANGISHSNSKHNILWTDMTNFLWLFTSEDGGVTWSSPK
                     DITAGLKKDWMGFLGTGAAPGLEIDAKDAEGQSFKRLLFPVYYTKQQGITGPESLGRS
                     SSANVYSDDGGLTWHLGESPNDGRIFVDNQHTNSKDFNTSVTELTENQIVQLNNGYLL
                     QFIRNTGKTVMIAKSIDYGKTWDDVLIGSKIPEPFVNLSVIHYELNNKEYIILSNPVG
                     HPDAESTDMRTRNIRMKGALRIGEVQSDDSIKWVASTMYEPRRFAYSSLVQLDDENVG
                     VLYEYNGHIKYSTFNVKEMIEEGKRFDISYIDSEIEAESGNDKNNIQKGDVLKFTVTM
                     NQPMFIVGERILDFTIGGEKRKAIYVEGDQSEYIVFSYSVTEQDEGIIKVLSTFEGFT
                     VENRYNVALMKDGSIEVGRVLTEVEVAEAAVEAAETASQAGVAKKTEVTADGLVSAAD
                     KVAVDELNEVTEAKTLNASMLELPKTGSVDNSILKVTGGVLFLGSLGLLGYKKRRENN
                     R"
     gene            6765..6920
                     /locus_tag="ACHALG_002536"
     CDS             6765..6920
                     /locus_tag="ACHALG_002536"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587867.1"
                     /translation="MSKEERPIECYILGQDNEYGMIEWLVDDVVRDWCILEFFASTQM
                     GSAGNIG"
     gene            7048..7197
                     /locus_tag="ACHALG_002537"
     CDS             7048..7197
                     /locus_tag="ACHALG_002537"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587868.1"
                     /translation="MKKPKKFNPKPKNDFEKGLFQRFEKACFLEKDDQKYSEKRKKFV
                     KRELH"
     gene            complement(7518..8819)
                     /locus_tag="ACHALG_002538"
     CDS             complement(7518..8819)
                     /locus_tag="ACHALG_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFN2587869.1"
                     /translation="MDHCTRKLLGLTDENLFFEEEWLETVEEEGFRTNLIHAKLSYIP
                     SHCRKCGIKNEGQIIKNGSHKTKVQLLPYRDTKTELRLVRTRFYCKECQSTFNAQTNL
                     VDENCYLSKELKVQIALELTKNTTRKEIADRYFVSDVTVLRVLHTCLKTYHPRFDTLP
                     SVLCFDEFKSMKSCSGKMSFVFMNGQTQQLIGVLENRRLAFLKPYFLKFTRKARANVK
                     YVVMDMNAPYFELVKAVFPNAKIVTDRFHIVQQITRALNQLRMKTMNSFQKTEPTKYR
                     RLKRFWKLLLKHAYDLDSSNYQYDRSFRRPMTQKAIVDELLSYDEQLTRAYETCQLLL
                     YHFKHKDSQSFFDTINSLDQRLPQWFCKKLTFLNKYKLGIQYALKTRYSNGALEGRNN
                     KIKVIKRVAYGYRNFHNFRARIYLIQGLIFQVKQKPVKQSA"
     gene            complement(8970..>9133)
                     /locus_tag="ACHALG_002539"
     CDS             complement(8970..>9133)
                     /locus_tag="ACHALG_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFN2587870.1"
                     /translation="EGITNAMIYPYSNGKIEAKNTHIKTMKRVSYGFKSFENMRIRIF
                     LINQLINVR"
BASE COUNT         3026 a         1323 c         1653 g         3131 t
ORIGIN      
        1 tagaaaaaag ccataaacta aaaaatggtt agttgaacga aaggctattc atgaaataaa
       61 aaaaatcctt catgaagtcg agaaatatga aaaatatgat gaaaaagaac ttgataatga
      121 attaaaagaa ataaatgatt tattactttg attaaattga cacattgtca ataatgaaaa
      181 taggactgaa gaatcaattt tatttagatt aatataaaaa tcaatcaaac attacgttta
      241 attggttttt atattttttc aaatgtattt tacgtttttc atttgcaaca actgttggaa
      301 caactttcat aagatacgaa aagcgcttac gtatttccgc aatcagtata gtgagcttct
      361 taaactacaa aagagcgata tttagagtaa gcaatttgat cgacaatcga tgaaacctga
      421 ttaagatcta aatcggcacc aataacagtc acttggtaaa attggtcata attttttcct
      481 aattgtggga gattagcatt cgaaattaac aaatcaaacg gctcgttctg agtataagat
      541 aaaacagtaa cattttgttt cccttttaat tcatttttta ataagtatat tatagcatct
      601 gataaaagat gagattccat gtttaaaaca ccaatacgaa ctttgtaggt ttttttagga
      661 gataacatat aaattaaaaa tattagtcgt tttttcaaat agttcatttt tgaaggatta
      721 aataaattgg ttggctcaat aatatatttg agtatatcta atacaaattg atttctaaac
      781 gaattgttaa ataaaccata gtagtaattc aaagtcactt tgtctattga atagtttact
      841 ccatcaaaat aatagatttg cgcaattaaa gaaaaaactc ctgatttaat atcaattgat
      901 gaatgctctt taatcaagtt atacgtagac tgaattgctg ccatgacttc gtctgtgatt
      961 tcaaaaacag gattatttct agaatacaat cctaagcgaa tttcttcgta aggaacccct
     1021 ccaaacgtaa ttaaaaatag acatataaaa tacgtgacaa tttttgcttc atcatctgaa
     1081 caaatctttt tttgggaaca gtaggtatgc tgaacgtact gaaagattga tttattttga
     1141 taacgactga tgcttctttg ctcctctgga tttaagtttt taagtatggc tttattactt
     1201 atatttttca taagaacata aatccatatt ttcaattgtt tttgttgagc aatcgatagg
     1261 gttatttttt ttattagttt tgcaatgata ttatcaatta attcagaatc aattttttta
     1321 gaccccattt taaaaaaatt ataaaaaaaa taaatacgct gagttagact cccagataac
     1381 tttttattcc taatttttag ttctaaatgt tgcagtgaac gattgcattc agaaatcaaa
     1441 cgattcagtg tacctatact tatttttagt ttatcagtta atacaatagc atcatatttt
     1501 ttgtagtgaa ataaatactc cattatttga tatttaatgg atttttttaa caaaatatcg
     1561 gttagatcat ctagtgagac actatcgttc acttttaatt ctaaggtata acgattaata
     1621 ataaatgaaa ttttttcatt ttttagttgt cttttaaggt tatcggatag tttaataaga
     1681 gttggcatcg taatatttaa ttcctgacaa gcttcgcttt ttttgatatg accggtagat
     1741 aaatacagga atttcagtaa cgtgtactct aatcttaaat ccttatcaag taagtcttct
     1801 ttatacacga ctttctccct cctgtgtaaa tccttattct ttcaaaatag tttcaatcaa
     1861 ctttaatcct attcattaat ttttttattt actcccgaaa aacaaattgc tttttaacaa
     1921 gaactattta aaaaactaaa aattataact atgtatttga aaaaattata gacaatttcg
     1981 tttgtcaaat taagttagac aaatcatctt ttgtgacgta actcaaaaat acttccattg
     2041 gtgtccgata gtgtaatgat tttctaggaa tatgatttcg tttcgatgcc accgattgga
     2101 taaaggtttc agatacacca ttaaaatcca tagctttata caaaccatct ttacgtaata
     2161 agccatttga atgttcattc aatcctcgtt gagaaggtgt tcctggatcc gcaaaataaa
     2221 ttgaaatatc gttggtatta ctaatctttt tccaatttga gaattctttt ccacaatcaa
     2281 atgtgatgga tttaaataag ttttttggga cacaacgaaa ccattgattc agcctatttt
     2341 caatgtcttt agcctgtctg ccttcgggtt tcaaggtaat gatgacttta gatagccgtt
     2401 caactaatgt gatgaccgcg cttttatgtt ttccacccac gatagtgtca ccttccaggt
     2461 gaccaaattc atgatcaaac tgggtatagt ctgtctttct gtctcggatt gaccggcgaa
     2521 acccttgttt tcctcttttt tctttatgac cgtttggttt tctttttccc ttcattggta
     2581 aatcgtgagt gtcaaaggtt ttatctttaa acattcgata cagtgtgcgt accgaacaag
     2641 caataggaaa ctctgctcga ccaataagga catcaggagc ccacccttga acaacctttt
     2701 tttgaatgta ttcctgttgc tcatcaggta aaacaatcgg atgtcttccg cagttacgtt
     2761 tgtttttctt atattgttta tagtagctca ttgcgcttcc accagattta agaaaacgat
     2821 agaccttata aatagtttgt cgagaacgtt gtaacagatt ggctactttt gaaacaggtt
     2881 gtgattgatt gaaataagct tctatcatta cgagttcagt tggtgtaaga tggttatagg
     2941 tcatttgtac tcactcctta taattctttc gtcggaacta ttttgagtgt agcacaaatg
     3001 acttttttta gttgtctagc ttaattttac aatcggcgca attaaaaata aatccacgtt
     3061 attatgcata tcttgagagc gcttttctcc aataaaataa atatattata ataataaaca
     3121 atgataacac aaaaataata ttaagcaaat taaagcaact ggatattttt tgctttgtta
     3181 tttttgtgaa ataaccgtat taaattctaa tctacgcaaa aagaacctcg ttagaaaata
     3241 agtagctatt ttctaatatc caggattgca ttaaatatga acagttaagt cgagccacga
     3301 ttagtcgaca tattaggaaa aaaagccagt cattagatgg aagaacgttc tctatctact
     3361 agtactttat gtatacattt atatacgaaa gcaggcaaat tgttggctgt gtgttataat
     3421 gtattaaagt atttagaatc tggtatatat aatatccatt atagtgctgt tcctatagat
     3481 ggtaatacaa gtggtggtat tacaccaaca gaaaaaatag ctagaaaaac agttcgtttg
     3541 attgttttac caaatggtgg attagttggt aaaacagggg cggcattatc atctgtggtt
     3601 ttgccagaag gttggtcatg gaagaatgca aatgaaatta tagcagcaga ttccagttat
     3661 tctttgctgt atacaaacgc taaagatgat acagtggatg aaacgaagac tatatgaaat
     3721 aacgattcct tcaatgtata tcactgaaga accaattaat attgatcaaa gtgatagaaa
     3781 tattttagta cttgataata tgattttaga tggagaaaaa gctacaattg atttgattag
     3841 taacaagaat aacgataaaa tcgacacaat tatgagtatg cgtacaggaa gctttacttt
     3901 ccgttacagt cttgattctg agtctacgat aaccaatagt gaattaacta ctttattttc
     3961 tatttcatat ggcgcagctg aatctaatta tttctctttt tatatacgtc ctaaaaatgg
     4021 aaaaattgga attgaagtta gacgcaatgg aaaatcttat cttaaagaag ttggaacagg
     4081 cttcaaccta ttaaataatg ctgattggca ttttattacg tatacgttta atggtgtgta
     4141 tatgcaggtt tatttagatg gtattttaca tggagaaacg tcatttacag gattatttta
     4201 taataattca tgggctgaac atgcggatag attatctgta ggtggtacag ttagaaagaa
     4261 taatgttcag tggcggttaa aaggcttact agatactgta attgttactc gagatgtttt
     4321 gtctcctgat gaaattaaaa cgctccatcg tgtcacagat aatgaagcta ttggtgaaaa
     4381 atctagtatg tgggataaat acgatgaaga tattttcgaa tatcgtattc cttctatagt
     4441 taaattacct gatggaacat tagtggctgc tgcagatgct agaaaaagac attaaactga
     4501 ctggggagat atttctactg ttgttcgtat cagtaagaat ggtggaaaaa catggagtaa
     4561 aaatataaca gtactggata tgcctacgca gccatatttt acttcagaat atttgacaac
     4621 agattggaat accaaacata cgcaatctgc attttcaatt gatccaatac tattagctga
     4681 tagttcaggt aaattatatt tattagtaga cgtattccct gaatctcgag gttctattca
     4741 ttcgaagcct ggtgatggct atacactcgt tgatggaaaa tattatttga cattgaatga
     4801 taaatctaaa aaaacaatat actgttcgtg atcatggaat cgtatatgat tcattaaata
     4861 aaaaactaaa tggcgtattg atgtaggaac atttgataca gcattttcta ctcgtggtga
     4921 tatatatgag caacaagaca atggtagcga aaaacttgtt gggaatatat ttatgcgttc
     4981 aaaaaatgat gctaatggaa tttctcattc taattctaag cacaatattt tgtggacgga
     5041 tatgactaac tttttatggt tgtttactag tgaagatggt ggtgttacat ggtcttctcc
     5101 aaaggatatt actgctggct taaagaaaga ttggatggga tttttaggaa caggagccgc
     5161 acctggatta gaaattgatg caaaggatgc agaaggtcag tcattcaaga gattactgtt
     5221 tcctgtatat tatacaaaac aacaaggaat aactggacca gaatcattgg gacgttctag
     5281 cagtgctaat gtttatagtg atgatggtgg attaacttgg catttgggag agtcgccaaa
     5341 tgatggccgt attttcgtag ataatcaaca tacaaattca aaggatttta atacatctgt
     5401 taccgaatta acagaaaatc aaattgtaca attaaataac ggatatttat tgcagtttat
     5461 acgtaatact ggaaaaactg tgatgatagc aaaaagtatt gattatggta aaacttggga
     5521 tgatgttctt attggttcca aaataccgga gccatttgtt aatttatcag taattcatta
     5581 cgagctgaat aacaaagaat atattatatt atctaatcct gtaggacatc ctgatgctga
     5641 atcaacagat atgaggactc ggaacataag aatgaaaggt gctttacgta ttggagaagt
     5701 tcaatcagat gactctatca aatgggtagc aagtactatg tatgaaccaa gacgttttgc
     5761 atattctagt ctagtgcaat tggatgatga aaatgtaggt gttttatatg agtataacgg
     5821 tcatattaaa tattcaacat ttaatgttaa agagatgatt gaggaaggaa agcgttttga
     5881 tatttcttat attgacagtg aaattgaagc tgaatcagga aatgataaaa ataatattca
     5941 aaaaggagat gttttaaagt tcacagtaac gatgaatcaa ccgatgttta ttgttggaga
     6001 gcgtattctt gattttacta ttgggggaga aaagcgcaaa gctatttatg ttgaaggcga
     6061 tcaatctgag tatattgttt tttcttactc agtaacagaa caagatgaag ggattattaa
     6121 agttctttct acttttgagg gttttactgt agaaaacaga tacaatgttg ccttaatgaa
     6181 agatggttca atagaggtag gtagagtgct tactgaagtt gaggtagcgg aagccgcagt
     6241 tgaagcagca gaaactgcaa gtcaagcggg agtagcgaaa aagacggaag taacagctga
     6301 cggtttagta agtgcagcag ataaagttgc agttgatgag ctaaatgagg taacagaagc
     6361 gaaaaccctc aatgcttcga tgttagaatt gccgaaaaca ggttcagtag ataatagtat
     6421 tttaaaagtt acaggaggtg tattgttttt gggatcttta ggattgttag gttataaaaa
     6481 aagaagagag aataatagat agattgtttt ttagaatgtt aaacaagcaa ttgattaaat
     6541 aattaaatca gagttagaat gaatctcatc ctaactctga tttttagttc tcttactttg
     6601 atttaattga tttatcaaaa agattctaat tctcgtgttc ttaacgattt gaatttatag
     6661 aatacttgtt ttatatagtc taattcacaa taatggactc cttattatta tgaataattg
     6721 ctttttcatc taactaattg tccaacttat ttaaaaaaga tgaaatgagt aaagaagagc
     6781 gaccgattga gtgttatatt ctaggacagg ataacgagta tggcatgatt gaatggttag
     6841 tcgatgatgt ggtaagagat tggtgtatct tagaattctt tgcgagtacg cagatgggga
     6901 gtgctggaaa tatcggttga aaacgtccaa taaaacagac tttgatattc gcaagattca
     6961 tattctatta taaaaagtct tttataacga ttgttatcct gataaattag tgataataaa
     7021 aagttgttta aaaggagtgt taccatggtg aaaaagccta aaaaatttaa tcctaaacct
     7081 aaaaacgatt ttgaaaaagg attgtttcaa cgttttgaaa aagcatgttt tttagaaaaa
     7141 gatgatcaaa aatattctga gaaacgaaaa aaatttgtga aacgagagct tcattaggga
     7201 gaaaaattat agctattgga gagattttgt aatcgtttaa gttaaatata gttgaaagta
     7261 tagagttgca ttcatttgtt tttgatagaa tgagaagaaa gcagaagtca ataatcaaaa
     7321 tgggactgaa gaatcaattc tgattcttca gtcccataaa ttatagagcc ccaaaggctc
     7381 tttgtcaata aggactgatg agttgtgcaa aatcaaatct gggtcagaat gaaccacatt
     7441 ctggctcaga ttttttgggc tcttcgacat ttagtgttgg tagagaaaga tacaactgaa
     7501 aatttagtca taaccagtta ggcagattgc ttaactggtt tttgttttac ttggaagatc
     7561 aagccttgaa tgagataaat tcttgctcga aagttgtgga aatttcgata gccgtaggct
     7621 actcgtttta tcacttttat cttgttgttt cttccttcta atgccccatt actatacctg
     7681 gtttttaaag catattgaat ccctaatttg tatttattta aaaacgtcag cttcttacaa
     7741 aaccattgtg ggaggcgttg atctaagctg ttgatcgtat cgaaaaagct ttggctgtcc
     7801 ttgtgtttaa agtgatagag gaggagctga caagtctcgt aagcccttgt taattgttcg
     7861 tcataactca aaagttcatc gacgatcgct ttttgcgtca ttggtctacg aaaggatcga
     7921 tcatattgat aattggaact atccagatca taagcatgct taaggagcaa tttccagaat
     7981 cgcttcaatc gtcgatactt tgtcggttcc gttttttgaa agctgttcat tgttttcatt
     8041 cgcaactggt ttaacgcacg agtgatttgt tggacaatgt gaaagcgatc ggtcacgatt
     8101 tttgcgtttg ggaagaccgc ctttacaagt tcaaaataag gggcattcat atccatcacg
     8161 acgtatttca cgttcgctcg tgcttttcgg gtgaatttaa ggaaatatgg ttttaagaat
     8221 gcaaggcgac gattctccaa tacgccgatc aattgttggg tctgcccatt cataaagaca
     8281 aaactcattt ttccgctgca ggacttcatt gatttgaact catcaaaaca aaggaccgaa
     8341 ggtaacgtat caaaacgagg atgatacgtt tttaaacagg tgtgtaggac acgcagaacg
     8401 gttacgtcag agacaaaata gcgatcggca atttcttttc ttgttgtatt cttggtcagc
     8461 tccaaggcga tttgaacctt taattctttt gaaagatagc agttttcatc caccaagttt
     8521 gtctgagcat tgaatgtcga ctgacattct ttacagtaaa agcgcgtacg taccaagcgt
     8581 aattctgttt tggtgtctcg ataaggcaaa agttggactt ttgttttatg tgagccattc
     8641 ttgatgattt gtccttcatt tttgatgcca catttacggc aatggcttgg tatatagcta
     8701 agtttggcat ggataagatt ggtgcgaaac ccctcttctt ctacagtctc taaccattct
     8761 tcttcgaaga ataaattttc atctgttaaa cctaacaatt ttctagtaca atgatccatg
     8821 gagatatcct ttcttggtgt agttttgttt tagtcgacta aattctacca gttaggctat
     8881 ctctttttct atcttttttt acaaaaaaag tgtcggtaga aaatccgtag attttccacc
     8941 aacactaaat attgtagaac cattttttgt tatcttacat tgattaattg attgatcaaa
     9001 aagattctaa ttctcatgtt ctcaaatgat ttaaatccgt aggatactcg tttcattgtc
     9061 tttatgtggg tattcttcgc ttctattttt ccattggaat aaggatagat cattgcgttg
     9121 gtgatgcctt ctt
//
LOCUS       DAZABD010000091         8747 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000091 DAZABD010000000
VERSION     DAZABD010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.093"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            8..322
                     /locus_tag="ACHALG_002540"
     CDS             8..322
                     /locus_tag="ACHALG_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587871.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
     gene            877..950
                     /locus_tag="ACHALG_002541"
     tRNA            877..950
                     /locus_tag="ACHALG_002541"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:911..913,aa:Ile,seq:cat)
     gene            complement(1466..1768)
                     /locus_tag="ACHALG_002542"
     CDS             complement(1466..1768)
                     /locus_tag="ACHALG_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFN2587872.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
     gene            2076..3368
                     /locus_tag="ACHALG_002543"
     CDS             2076..3368
                     /locus_tag="ACHALG_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1251 family transposase"
                     /protein_id="HFN2587873.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIY
                     TAQECIHCKHQIASDIVRWGTTTVRLLMNDVSEYRTYLELKKQRFKCKACQRTFVADT
                     SVAKKHCFISEKVRWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRTP
                     LPKVLCFDEFKSVRGVSGAMSFIMMDGQTQRLLDIVENRQLPFLKRYFSHFSREIREA
                     VEWIVIDMYAPYVSLVKKLFPKAQLIIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQK
                     HYQLGKQLKRYWKLLQKDERKLDYTRRLWRPGFKAHLTETDIVDRLLKGSPALRVGYQ
                     LYQDFLYAVKERDYVSFEELLTNNIMLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPL
                     ECLNNHIKVLKRNAYGFRSFYNFKLRIMIRHGNALIFN"
     gene            complement(3495..3861)
                     /gene="ssrA"
                     /locus_tag="ACHALG_002544"
     tmRNA           complement(3495..3861)
                     /gene="ssrA"
                     /locus_tag="ACHALG_002544"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(4032..4805)
                     /locus_tag="ACHALG_002545"
     CDS             complement(4032..4805)
                     /locus_tag="ACHALG_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFN2587874.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            4949..5299
                     /locus_tag="ACHALG_002546"
     CDS             4949..5299
                     /locus_tag="ACHALG_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFN2587875.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            5280..5996
                     /locus_tag="ACHALG_002547"
     CDS             5280..5996
                     /locus_tag="ACHALG_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFN2587876.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            5996..7468
                     /locus_tag="ACHALG_002548"
     CDS             5996..7468
                     /locus_tag="ACHALG_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFN2587877.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKKKSQVQTPV"
     gene            complement(7495..8667)
                     /locus_tag="ACHALG_002549"
     CDS             complement(7495..8667)
                     /locus_tag="ACHALG_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS256 family transposase"
                     /protein_id="HFN2587878.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYYERSFTTRVGTLELKVPRTRDGHFSPTVFERYQRNEKA
                     LMASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKN
                     YPYLMTDVLYIKVREENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWTTFFEY
                     LKERGLQGTELVISDAHKGLVSAIRKSFTNVSWQRCQVHFLRNIFTTIPKKNSKSFRE
                     AVKGIFKFTDINLAREAKNRLIHDYIDQPKYSKACASLDDGFEDAFQYTVQGNSHNRL
                     KSTNLIERLNQEVRRREKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
BASE COUNT         2797 a         1554 c         1607 g         2789 t
ORIGIN      
        1 atagcttttg gatttttaca gtaattttat cttaataata gcaatattat tacttctaaa
       61 tatttggttt tttgataaga gtagaaatgc agggataggt tttagaacca aacgaagtac
      121 gtcttctgaa aaaaagtggg tatattcaca gactattttt tacggagggg ttatttcaat
      181 cagtttactt tcttcaacac tttattcttt taatgttata gacgtttcca tgtcaaattt
      241 tatatctatt attggaatcc ttatttctgc aattattaca caactacttc ttgtgtttga
      301 agagaagtca aaaaataatt aaagcaatta tgccttttag tacaggaatt attacttctt
      361 acaaatgtat agcgaatgtg aagcctgctg ttttaattgt tctagaaaaa cttttgagtg
      421 aacaattatt ttatgttagg atgaattaat ttctctagag tttataatta aaaaacttat
      481 ttgaaggaac atgcttaaac cataattgtg tttttccttc catcagcata aaagttcacc
      541 tcaaaaagag tgtacgaaca aaagttcttt ttgtaaacct taaaatagat tttcctgaat
      601 acgtattggg gatttctaaa tgtttgaatg tttttttgaa tgcaaaatca atgcaaaaaa
      661 ttttgtattt ctctactttt ctctccttga ccaaaataaa aaaacgttga tttaacaacg
      721 tttttcatac atgtttatct gtctcaacca tacatatatg gagacgcata tcttcactat
      781 cactcataaa tagtcacact ccaagatatg cgataacagg tttgttgcca cgcattctac
      841 tggttgattg tttatggctt tatgtggcaa caaccactac ccttagctca gttggtcaga
      901 gcagacggct cataaccgtc cggtcgtagg ttcgagtcct acagggtaca ttaatgtagc
      961 caattgaatc gttctgtgtt agaatttttt gaagagtatt atacaagcta aagcttttct
     1021 tcattgccac tcaaatgagt ggctttttta tgtatccttt tatggattaa tgaaaggatg
     1081 tttcacatag ttatacttct gtatatttga aaagttttac tttgattttt aaatggaaag
     1141 acatttgggt tatattgtga gataataata aagaagagtt taaagcgttc cccaaaaacc
     1201 acttccccat aagtgtgtta cgctttaaac tcttttatat ttgaagccat taaaaagcat
     1261 accatataac tgtaaaaaat aatgggaaaa agacttataa ttggagtggt agttaattag
     1321 tgacttattt ttgattttat agcactgata ctataaaata tagatatcat catattacac
     1381 aatcttaata ctaacttaaa aaatatccct tttataagta tggtgataaa atccgttccg
     1441 ggctaccttt tttaggtagc ctactttaat ctttatacct ttctggatca acgaaagtat
     1501 acttcaaata gtcataacgc cgatgatcgc tccgtgcgtc tggcacatca gtcacgatat
     1561 caaacaaaaa atacacatct ttcttcattc tggtttttgt tgccggaatt ttaaagtagt
     1621 tcttattaga gtaatagaga ttgattaata agctatcttc gattgctaaa aagaaaactt
     1681 ctgaatccca caccttataa aaatctttga caaatctatt cgaaggatca aatttaaacc
     1741 ataattgtgt ttttccttcc atcaacataa tgttcacctc aaaaagagta tacgaactaa
     1801 tgttcttttt gtaaagtagg aatatcattg ataatatgga tggaacatat atattaaaaa
     1861 gtgttctttt tgaatgctat tttgaatgcc attttcattc atttaacgaa aatctgcgaa
     1921 aataaaaaat gctcaaaccc ttataaaatg gctctatact ttttgatgtt ggtggatgac
     1981 accagcatct tttttatttt ccaaaacata tagaaaagac accttgaaat cctttagaat
     2041 taatttggcg aaccaaccca aaaggaggaa tcaagatgtc ttacactcac cttatcaaag
     2101 aaacattaga tattctagac ttatcagtta cttttaacga aaattgtctc acaaaagaaa
     2161 aatacaaagg gcaaatctgt catatttatc gtggaaactt gatttataca gcccaagaat
     2221 gcatccactg taaacaccag attgcttcag atattgtgcg ttggggtaca acaaccgtcc
     2281 ggttgttaat gaacgatgtt tctgaatacc gtacctacct cgaattgaaa aagcaacgct
     2341 tcaaatgcaa agcttgtcag cggacctttg tcgcagatac ttctgttgcg aaaaaacatt
     2401 gtttcatcag tgagaaagtc cgctggtctg tcgtgactcg tctgaaaaaa aatacctcaa
     2461 tgacagagat cgccgctcag aaaaatctat cggtctcttc tgtttaccgt attatgaagc
     2521 gattttatcg tccgttgaat ccttttcgga caccgttacc taaggtactt tgttttgatg
     2581 aattcaaatc agttcggggt gtttcaggtg ctatgagttt catcatgatg gatggacaaa
     2641 cacagcgctt attggacatt gtcgagaacc gacaactgcc ttttttaaaa cgttatttta
     2701 gtcatttttc acgagaaatt cgtgaagcgg tcgaatggat cgtgattgat atgtacgctc
     2761 cttatgtatc gctcgttaaa aagctgtttc caaaagcaca gctgattatt gaccggtttc
     2821 acatcgtcca acatattggt cgtacctttc gcaatcatcg aatcaaagaa acgaaccaac
     2881 tattaaaaag caaagaacaa aaacactatc aattaggtaa gcaattaaaa agatactgga
     2941 aacttcttca aaaagatgaa cgcaagcttg attatactcg acgcctttgg cgacctggct
     3001 tcaaagctca tttaacagag accgatattg tggatcgctt actgaaaggg agccctgctt
     3061 tgcgtgtagg ctatcagtta tatcaagact ttctttacgc tgtaaaggaa cgagactacg
     3121 tatcgtttga agaactactt accaataata tcatgcttcc cgaaggctat cagacgacct
     3181 tgagaacgtt ccagaaattt ctgccacaaa tcaaaaatgc tttacaacaa agttattcaa
     3241 atggcccact tgaatgtctg aacaaccata tcaaagtgtt aaaacgaaat gcatatggct
     3301 ttcgtagctt ttataatttc aaattgcgca tcatgattcg tcatggcaac gcattgattt
     3361 tcaactaaaa aagatcaaga ggaactgatc cacttgatct ccaattattc taagatttct
     3421 ctttaccaac atcatttgac aaagagccta taaaataagg atttaagcat ctagcgaaat
     3481 ctgctgaaat ctcacaatgg agacggcggg agtcgaaccc gcgtccaaac acatcgccac
     3541 ttaaaaatct acgttcatag tcaactcatt taaggattca ctttacagct tgccgagtga
     3601 caggcattct gtatcgctag tctgatgttc tctttttcaa cttacagacg gaaagttgaa
     3661 acgtatccca ctaaagttag gacccttact cgagcacatg ggcgatgccg agaggatcta
     3721 cgctaactgt ttttaggcag ctaaagcgta agagttgttt tcgtttttag cagttatatt
     3781 taactgtaac gtttttacgt agacgtaacc tacgaaacgc aattcaagct cgactgtgcc
     3841 tgtcgaatcc gtaacgtccc cgttagctaa caagggttac gtgtattttt catacaaaat
     3901 tagtatagca tatattggga taaattcaat aaaaaagcga atcatatccc attatttata
     3961 taataactgc ccctttctta agaaaaaata agcgattttc ttaagaaaag aggtagcttc
     4021 aaaaaaagat cttaaatcag atcaaaatac ttcaatgcgt ggacaattcc gccttctgta
     4081 tttttctttg tgataaaggt agaaagttct tttaatccgt cataggcgtt gcccatagcg
     4141 attttgttgt cgcaagcttc caacaaggca aaatcattcg gaccatcgcc aaatccaaaa
     4201 gtaggaactt tttccagctg aagttctttt tgcagacgtt tgacacctgc cccttttgag
     4261 attcctttct tcactgtatc gatcgagtaa ggaccattcc gataaaaagt tagctcagga
     4321 aaatgttcgt gatagtattc gtcacctgat tgagataaga tcagcaacat atttactgtg
     4381 ttttctttaa agcctaaagg atcaatttcc ggaacatctg aatgaatgaa gtcgtaagca
     4441 ttgatcattg tttggctatg accggtacac cagattttct gatcgttata gtaagaaagt
     4501 tcatgaccgt tcaactgtac atgatcatac attttttgac attcttctga agaaaatgtt
     4561 tgagaataga tttcttcgcc gtctactcga atgaaagaac cattcatggt gaccgcagaa
     4621 gtaatgcccg catcttccat gatagagtgg atttctgctt ctgtacgtcc tgtagcgatg
     4681 acggggagaa cattgttttc ttttaatgcg ttcatagctt ttgccacttc gggtgtgatt
     4741 tttgatttcg catctagtaa tgttccatca agatcaaaga aagtgattgc acgataattt
     4801 gacacaaata aaactccttt tttattggaa taagtttatc ttaacaaaga atgaacaaaa
     4861 gacaaacctt tcgtgaagaa ttcctgaaca tattggttca gtcaacaatt tagagtatac
     4921 tataagaaga gaatttagga gggaaagaat gatttttttc agtattggta tcttattctt
     4981 aataattggc agtctttttc ggattctgcc ctcaaaagga aatctgcctt tttatgggta
     5041 tcattcgcct cttgccgcaa aaacagacgc tcattggcgg ctggctcaaa agacaagcgg
     5101 aaactggttt tttcttatgg gcttactgat ggcattgatc ggctattatt taaagacatc
     5161 tgggcatacg aattattttc tgattgaaat gcttctgcta gtatttccaa ttatgcctat
     5221 ttttattatg acagaaaaga aattacagaa atatgacctg gaaactggag ggaacgataa
     5281 tgaatatttt aatgattgaa gataatcaat ctgtttctga aatgatgcag atgtttttct
     5341 taaatgaagg ttgggaagca actttcaagt atgatggaaa agaagggctc gatgcctttt
     5401 tagaaaaccc acaacattgg gatatgatta cactagacct gaatttgccc acactggacg
     5461 gtatgctagt aggaagagag atccgcaaag tttcaagtac tgtgccgatt atcatgctga
     5521 cagctagaga ttctgaaagc gatcaggtga tcggcttaga gatgggggca gatgattatg
     5581 tcacaaaacc tttcagtcct ctgacgctta ttgctcgaat gaaagctttg catcgccgta
     5641 gtgagttggc agaagcaaga gaagaagcag tagtttcaga tgatcatttt gatatcgaga
     5701 ctagtcattt caagatgaat acgaaaacac gagaagcgta tctggacaac cggccaattg
     5761 aaggactgac gcctaaagag ttcgatctat tgtttacttt agcaaaaaaa ccacgtcaag
     5821 tattctctcg ggagcagttg cttgaattag tctgggatta tcagtacttt ggtgatgaac
     5881 ggacagtgga cgcccatatc aagaaattac gacaaaaaat cgaaaaggtc ggaccacaag
     5941 tcatccaaac tgtttgggga gtcggctaca aattcgatga ttctggtgtt gcctaatgcg
     6001 ttatttgtat cagcaactct tagctttctg cggaatggtc gcgttgatct tattgatcgt
     6061 tggagtttct tttacacaac tgacaaaaca aacgattgaa gaaaacaatt accagcaact
     6121 gtttggatat gctgaatcag ttgaaaagac aacgcaaacc tatgccgata cgctcccgca
     6181 gtttagtcaa gatcaggcgt ttcaaaatgc attgctgctg acagagcaag tactgacaga
     6241 acaaaatgtg aactttatat ttatcgataa aaacgaacgt gtcatttatc cgactaatac
     6301 agctttactg aattttacct taacagctga tcaatggaaa aatctgagga atggacgtca
     6361 agaaaaattc acatcgaata aaaatatttt aggacaagca caggcgacct cttacgcact
     6421 tgtgccattt aatttgaatc atgaattcta tggagcactt gtagttagtc aacctgcgcg
     6481 aaatatcgat aacagcgttc gtccaattac actgaacctg ttcaaaggat ttattttttc
     6541 cagtatcgtt gcagttattg ccagctatgc ttttgcagca ttccaagtta agcgtatcaa
     6601 tcggttaagg aatgcgacga aagaagtgac gaatggtaat tttgatgtac agctccccgt
     6661 acatgacaaa gatgaatttg atgagttagc agatgatttc aataaaatga cgaattcatt
     6721 gaaagaatct caagcagaaa ttgaagaaca agaaaatcgt cgaagacaat ttatggcaga
     6781 tgcttctcac gagatgagaa cacctcttac gacaatcaac ggacttctgg aagggttaga
     6841 atacaatgca atccctgaaa atcagcgaga aaacgcgatt aaattgatga aaaatgaaac
     6901 agagcgtctg atccgtttgg tgaatgaaaa cttggattat gaaaaaatcc gtacgaatca
     6961 aatcagtatg gtcattaaaa aattcaatgg aacagaaaca ttgaaaaata ttgttgcaca
     7021 gttagaagct aaagcagaag cagcaggaga tacattgaca ttgaaagccg ttgatgatat
     7081 tgatgtttac gctgattatg accgatttgt ccaaatcatg gtcaatatta ttcaaaacgc
     7141 gatacagttt actgaaaacg gccaaatcac gattacttta gaaaaaggct atcttgaaac
     7201 gatcatcacg attgaagata cgggaattgg aatgtctgaa caacaaatga aaagtatctg
     7261 ggatcgttat tataaagtag atccttcaag aaaaaataca aaatatggtg aatcaggatt
     7321 aggtttacca atcgtgcagc aacttgtacg cttgcataaa ggaaaattag aagtagaaag
     7381 cgaactagga aaaggaacaa aattcgctgt tcgtttgcct gacgtagaaa tcaaaaaaaa
     7441 aaagagtcaa gtccagactc ctgtgtaaaa tgctatacaa tgtttttacc atttctactt
     7501 atcaaaattg atgtattttc ttgaagaata aatccattca tcatgtaggt ccataagaac
     7561 ggctccaatt aagcgattgg ctgatgtttg attggggaag atgcgaataa tcttttctct
     7621 tctgcgtact tcttgattca gtcgttcaat tagattggta ctctttagtc gattgtggga
     7681 atttccttgt acggtatatt gaaaggcgtc ttcgaatcca tcatccaatg atgcgcaagc
     7741 ttttgaatat tttggttgat cgatataatc atgaatcaat cgatttttag cctcacgcgc
     7801 taagttaata tctgtgaact taaaaattcc tttaacagct tctctgaaag attttgaatt
     7861 ttttttagga atggtggtaa agatatttct taggaagtga acttggcatc tttgccaact
     7921 tacgttggtg aaggattttc taatggcaga gactaatcct ttgtgcgcat cagaaataac
     7981 gagttccgta ccttgtaaac cgcgttcttt taggtattca aaaaatgttg tccaggtctc
     8041 ttcgctttcg ccactttgaa tcatgaagcc gataatttca cggtcgccat ctttggttat
     8101 tccaatcgct atatgacagc tttttgagag tactcgattt tcttctcgta cttttatata
     8161 gagtacatcg gtcattaagt aaggataatt tttttctgat aataaacgat tctgccactc
     8221 gttaaccata ggttctagct gttctgttaa gctagaaacg aaggacttag agacggattt
     8281 accacaaagt tcttccacaa tttttgatac tttacgagtt gaaacgcctg atacatacat
     8341 ttccaacatt gaagccatga gggctttttc gtttcgttga taacgttcaa acactgtggg
     8401 tgaaaaatgg ccatcacgtg ttctgggtac ttttaattct agcgtgccta cacgtgtcgt
     8461 aaagctgcgc tcataatagc catttcgttg actttgtcgg ttttctgttc gttcatattc
     8521 ttttgcttga atatattctg ttcgttgatt ttccattagt tgattaaata ccgttgttaa
     8581 aatattttta gaaacgtcat cctttacaga atattcaata atgctttgaa tctcttcgct
     8641 tttcagtgta aaatgtactt gggtcatgta aaagtcctcc tgggtatgtt tttgtcgtta
     8701 aaaacattgt accgtaaaag gactgttata tggccttttt actttta
//
LOCUS       DAZABD010000092         8555 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000092 DAZABD010000000
VERSION     DAZABD010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8555
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.094"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(170..3319)
                     /locus_tag="ACHALG_002550"
     CDS             complement(170..3319)
                     /locus_tag="ACHALG_002550"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HFN2587879.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWSNLRQ
                     KIISNNQAELNDSPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIERENPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIIQIELK
                     QVTAKDGFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGVSSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMESVKKESYLDPTVFEAEEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKESDWLIKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTAWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVQSSTLNNQIQLLEE
                     QIGVYETVKGSLMEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDNLIHA
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(3333..4550)
                     /locus_tag="ACHALG_002551"
     CDS             complement(3333..4550)
                     /locus_tag="ACHALG_002551"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFN2587880.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLGDMMDVTSVKRIHQSDWTNSG
                     IRFLRARDIVSAAKNEEPSDYLYISEEKYNEYSKNSGKVSQGDLLVTGVGSIGVPLLI
                     TDDNPIYFKDGNIIWFKNEHKIDGNFFYYSFINNKIQKYIRDVAGIGTVGTYTIDSGK
                     KTPISLPTYDEQIKIGSFFKQLDHTIMLHQHELDLLKETKKGFLQKMFPKNGAKVPEV
                     RFPGFTGDWEERKLGEVVVFLNGRAFKQQELLDSGKYRVLRVGNFNTNDRWYYSNLEL
                     EEDKYANKGDLLYLWATSFGPEMWNEERVIYHYHIWKLKFLSKELDKQYLYTWLLTDK
                     EKIKESTNGTTMVHVTKGGIEQRTFQFPPDINEQQKIGAFFKQLDDTIALHQRKLDLL
                     KETKKGFLQKMFV"
     gene            complement(4540..6135)
                     /locus_tag="ACHALG_002552"
     CDS             complement(4540..6135)
                     /locus_tag="ACHALG_002552"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFN2587881.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYSISPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEAWLQGALEVFKHEQ"
     gene            6260..7045
                     /locus_tag="ACHALG_002553"
     CDS             6260..7045
                     /locus_tag="ACHALG_002553"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SinI family restriction endonuclease"
                     /protein_id="HFN2587882.1"
                     /translation="MPNKNRTFESILNEGLTTEPASIIFDEVIDDVEDSKYHKNLKEL
                     FYFALNNGPSLFTGISVPDSDNPLIMAKIYLTKWCNKYIRDRNNPALKKPLKTFGEKD
                     AALTTRVAANANIDDQKVLNDYLRGHFLYMSAENMNGSILEEYLAEVLEPEGWIWCAG
                     SVYRAVDFCYLGTSPILLQVKNKYNTESSSSSAIRVGTTIRKWNRLNKSTKISGLDSP
                     IPNWKALIEMTEASKELAAKLTENSYLAYINEKSTRELWTLDD"
     gene            7038..7280
                     /locus_tag="ACHALG_002554"
     CDS             7038..7280
                     /locus_tag="ACHALG_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587883.1"
                     /translation="MTNNILISIYPRYMEKIINKQKNFEFRNFYLTSDPYNIFWIYET
                     IPTKALKYKLIVKNPITKLKKNQHYFLGDDEFEQLV"
     gene            7688..8113
                     /locus_tag="ACHALG_002555"
     CDS             7688..8113
                     /locus_tag="ACHALG_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFN2587884.1"
                     /translation="MHLHRISALAVQIGRYHHSETRAVMEGLEEAIQTTNDCPYRRTF
                     HSDQGWSYQMKAYRNKLKEYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCF
                     EELKSAIDSYIYYYNNERIKQKLNWQSPVQFRKTTVTVV"
     gene            complement(8408..>8555)
                     /locus_tag="ACHALG_002556"
     CDS             complement(8408..>8555)
                     /locus_tag="ACHALG_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFN2587885.1"
                     /translation="NSLRYLEFREGAFDFQTSQQNPRKSLNYRTPLEVFLSCIDKDIL
                     SSLI"
BASE COUNT         2529 a         1711 c         1422 g         2893 t
ORIGIN      
        1 tgcaacagaa ccggtaaatg aatgaaaaag atggaagaat cattattcaa tagctttctt
       61 aaatactccg ttataaaatg tgaacaagta aaaagacatc tatccctatt ttctacactt
      121 ccactttact actatctttg aatcgtacaa ttttcttaac tcttctcgtt tacctcaatt
      181 cattttcaag tggaatcaac tcatcatcta acattttacg ccatgctcgc ttcatttctt
      241 gtgggtattt aaatggtttc gcttctggat gtttggcttt atatccttcg taatccttac
      301 tgttgataat tgctttcata ttaggaattg gatcttcacc tggcatatat tgcagattag
      361 aagcatgtaa ttctttctca gaaacaaacc actcattgga aaaggcatga atcaaattat
      421 cacttgcttg tgtaaaatag cgacgtttaa tgacaaagat atcttcatta ctatctaaag
      481 tccccacatc aaatgcattt aaaatttgtc ggtaaacttc tttgactcct tctgttttat
      541 tcaatttagc caaagctttt tcaatttctt ctcgtgcatc tgaactattc gcagcataag
      601 ttcctagtaa ttgatcaata taagtagaat caatatcata caacttcgtg gtattttcac
      661 tataaaattc gatcgttgaa aaatcttctc cctctttttc tggttcttcc tccatcaatg
      721 aacccttcac ggtctcgtac acgccaattt gttcttccaa aagttgaatt tgattgttca
      781 aagttgaaga ttgcaccatg tcatcattgt aatcatcata agtcactaac gattcatacg
      841 cacgattcaa ttcttgaaat gctttgacgt actcgatacg gatttcaagt ggcgtatgtt
      901 catcaatgga ttccatggat gaaacctctt ttaacttacc atatgcttgc ttgaatcttt
      961 ttttggattc ttggtaactt ggataaatca aacccgattc ttctttagct tctgaataca
     1021 gttttgtcgc atcctcgaca ttcttctcca ttgtcgctgg ctttcggaaa gtaacaatta
     1081 gtccttttgt tttttcagga tacgtacggt tcgtacgtga aaaggcttgg atcaatcctg
     1141 catactctag attacgatcc acaaataggg tttgaatcgt aggggcgtca aaacctgtca
     1201 atagacgatc cactacgatt actaaatcga tttgtttccc aaattgtttg aattccgctc
     1261 tttttcgtgc caaacgatta ttgatgtctc cgttataacg ctcaatgtct gctagtgacc
     1321 aggcagtatc ataatactga ttatattcct caataatttt ttgcatttct tcttgttggg
     1381 cacttgaatc tcggctattt tcttctaatg aataggtaat agcgattcgt gggaaatcag
     1441 gatcctccat cgttcgtcct tcacggatag gctgattggg aaactctttt attaaccaat
     1501 cagattcttt cgtcatctct ttgatggccc gataatactt tttggccata tcaatggaac
     1561 tcgtggttaa gatcgcagac ttcgttggac ggccatttcg aaaatcaaat tttgtatagg
     1621 cattgtctgg acggaacatt ttatgaatga ctttttggat atgttcctct gcttcaaaaa
     1681 cagtagggtc caaataactt tcttttttga cagactccat ttgatcgatc atacgattga
     1741 tctgttccgg tgaatactct gcatatgttt ctacttcttt taatttccga tagattttat
     1801 tctctaatga agtcggctcc acggtgtttt catgctctac ttgaaatcct aaaaccgagc
     1861 catcttctaa tgcattcttg atggtatagg tgtgcaagac ctctccatac tgatcatggg
     1921 tcgtacgtgc caattgccct ttggcttgtt tccgattctc agggaaaatc ggtgtacctg
     1981 taaagccaaa ccaagtggat ttcggaaaca tttttttgat ttcttccata ttttcagcac
     2041 ttaacgctcg atgacactca tcaactacaa acacgatatg ttgccccatt aatttttgga
     2101 agcgattggt tccttttttc tcttcctgtt tctttgcata tttcaacgcc gcatctagtt
     2161 tttgacgggt tgtaatgatc acaacattcg cattggcatc tgccaacaga gtttcactta
     2221 actctttggc acttcctgtt cccacgatca agctattcgc tttggcatta ccagaagaaa
     2281 ccccagtatt aaactccgaa gcaaatttcg taaactctgt ggtggtctga ttatctaaat
     2341 ctttgcggtc cactaacatg atcgttcgat cgatccccga ttttcgtgct aaaagcttag
     2401 tagatacaaa actagtcaac gtctttcctg aaccagtcgc atgccacaca taccctgatt
     2461 gatgtttatt tgcggcaata aacaaggctt gaatcgcatg cacttgataa ggatgtaaca
     2521 ccatgagggt tttattgtct tgatcttcac tgacaatcgt ataatccgca atcaagcgat
     2581 gggcatctgg gatatttagc acttgtttgc aaaactcata taagttttct acctttttat
     2641 tatctttcgt tcgccaacta aataaaaact taggatgcaa atccttcggt aaggcatttg
     2701 caaaataccg agtcgtttgt tcattggaaa ccacaaacaa ctgtaaggtc gaaaagatat
     2761 tgttccgaaa catgccttct tcagcatatt ttttgatttg attaaacgcc tgataaaaac
     2821 catcttttgc agttacttgt tttagctcga tttggataat cggtaagcca ttgatcaata
     2881 aggtgacgtc aaaacgtcga tcccgcgctt ctatgttact tccacgtttt gcaatttgat
     2941 ggaccacttc ataagtagag attcctccac caatgtcttg attggaataa aggataagtg
     3001 agacagaacc caattgcgga ttttcccgtt caatcgtgat ccgagccatc ccattttcac
     3061 cttttaacca tttcgcagca tcaaatgggg ttttcgtccg tagcaataat tcggttttga
     3121 tcgtttcaaa ctctttatcg gtcaaaggag aatcattcag ttctgcttga ttatttgaga
     3181 tgattttttg acgtaaattg ctccaaagat cttcttctga ttttagatcc ggacgatacg
     3241 tccactgatt atgtccttcc ccgaggacac gaatcaactg ttcttcgact tccgcttcct
     3301 cacggtgact aattttcatt cttcttccct ccctaaacaa acatcttttg caagaagcct
     3361 tttttcgttt ccttcagcaa atctaactta cgttgatgaa gagcgatcgt atcatctagc
     3421 tgcttgaaga aggcaccaat tttttgttgt tcatttatat ccggtggaaa ctgaaatgtc
     3481 ctttgttcta taccaccttt agtcacatgt accattgtcg ttccgttagt tgattcttta
     3541 attttctctt tatcagtcaa tagccaagta tatagatact gtttgtccaa ttccttagat
     3601 aaaaatttaa gtttccaaat atgataatgg tagattacac gttcttcatt ccacatttca
     3661 ggaccgaagc ttgttgccca taaatataag agatctcctt tattagcata cttgtcttcc
     3721 tctaattcta agtttgaata ataccatcta tcattagtat taaaattacc tactcttaaa
     3781 accctatatt ttccagaatc aagtaattct tgttgtttaa aagctctacc attcaaaaaa
     3841 actaccactt cccccaactt ccgctcttcc caatctcccg taaatcctgg aaaacgaact
     3901 tctggcactt ttgctccgtt tttagggaac attttttgta agaatccttt tttcgtttcc
     3961 ttcagcaaat ctaattcgtg ctggtgaagc atgatggtgt gatcaagttg tttgaaaaat
     4021 gaaccgatct tgatctgttc gtcataagtt ggaagtgaga taggtgtctt tttaccacta
     4081 tcaattgtat aggttccgac ggttccaatg cctgcaacgt cacggatata cttctgaatt
     4141 ttgttattta tgaacgagta gtaaaagaag tttccgtcaa ttttatgctc attcttgaac
     4201 caaatgatgt ttccatcctt aaagtaaatt gggttatcat cagtgattaa taaggggaca
     4261 cctattgatc cgacaccagt tacaagcaag tctccttgag aaactttacc tgaatttttc
     4321 gaatattcat tatatttttc ttctgaaatg taaagataat cggatggttc ctcatttttt
     4381 gcagctgaga caatatcacg agcacgtaaa aatctaatac ctgagttagt ccaatctgat
     4441 tgatgaattc gcttcacaga agtaacatcc atcatatctc ctaacttacg ctcttcccaa
     4501 tcttccgtaa aaccaggaaa ccggatttct ggtcgtgtat cattgctcat gtttgaacac
     4561 ctccagcgca ccttgtaacc acgcttcatt ttcaggtgtt gtttgcaggg aagagatagc
     4621 ttctaaaagt tctttttcta gcacttgctg ttcttgtcgt atcttcttga tgtcatttcc
     4681 gacatgaacc atatcaattg gttcttcttc ttcgaaagta tcgacatagc gtgggatatt
     4741 taagttatag tcattttctt tgatttcatc aaaggttgcc aaatgagcgt atttctcgac
     4801 atcttttctt tcagcgtagg tttcgaggat tttttgaatg ttttcttccg ataatttatt
     4861 ctgatttttc cctttaacaa attcacgact agcatcaatg aataatacat ctcgagtttg
     4921 acgattcttc ttcaatacaa tcaccgtcgt tggaatcgat gtaccaaaga acaagttcgc
     4981 aggcataccg atcacagcat aaatactgcc gtcttctaat aatttttgac gaatgacccc
     5041 ttctgcagca ccacggaata aaaccccgtg tggcaagacg atagccatag tccctgtttc
     5101 cttcaaatga tagaaaccat gaagaagaaa cgcaaaatct gctttggatt ttggcgctaa
     5161 tttcccgtag cgattaaacc gagaatcatc taagaaggtc gtatctgctg accaattagc
     5221 cgaataaggt ggattcatca ctactgcatc aaatgtgtaa ggttcatctg ttggccagtc
     5281 cttattcaac gtatccccat tgcggagatt catttcttcg gcatctaccc catgtaaaat
     5341 caagttcatt tttgctagat tgtatgtcgt tgtatttagt tcttgcccat gatatttaac
     5401 gttatctgga tgagttaagt agttacgtac atttaacatc aaggaacctg aacccatggt
     5461 tgggtcaaag acactaaaga atcgtcgttc tttttgatcc aacgtcacga tttgtgccat
     5521 catatcagaa accatatgag gcgtgtaaaa ttctccggct ttcttccctg cttctgaagc
     5581 aaattgactg atcagaaatt catacgcatc cccgatcaca tccccatcgt gacctaaaac
     5641 ttccacgtca ttcaatttct tgataacttc ggtaatcgtc acatttcgtt gttgctcatc
     5701 tgttcctagt ttttttgatt ggagatccac atcatcaaat aacccattaa attgattgta
     5761 ggtagatgcc aattgcacaa aggctttgtt tagatcgttc aattggaacg ttgcttgttt
     5821 tgcttgatcc gctaagacgt tgaaaagata ctctggtgag atcgaatacc ccaaaatatc
     5881 aacgattgtg gcaatcaggt cttcttttga ttcttcatct gaaagtaatt cacgatacaa
     5941 cattgtttgt ttgcttactg tgtcatattc ctctaaggat tcatctgcta aaagaaccac
     6001 ttgttccaac aaacggtcgg ataaatattt atagaaaatc agtcccaata aataattttt
     6061 atattctgac gcatccattt tactacggag attatctgcc gcactaaata atttttgatt
     6121 taattctgca cccaaaaaga tacactccta tctcttattt aaagttgcta tataccctct
     6181 tattttagca tactcccttt tagaaaaagc aaaaaaaaaa gaaaaaatct tattcttgat
     6241 aacaagctac gtgacgtata tgcctaacaa aaatagaact tttgaatcaa tcttaaatga
     6301 aggtttaacc acagaaccgg catccattat ttttgatgaa gttatcgatg acgttgaaga
     6361 tagtaagtat cataaaaatt taaaagaact tttttatttt gcactcaaca atggaccaag
     6421 tttgttcact ggtatatcag tacctgatag tgataatcca ttaattatgg caaaaattta
     6481 cttaacaaaa tggtgtaata aatacatcag ggataggaat aacccagcat taaaaaaacc
     6541 tcttaaaaca tttggcgaaa aagatgctgc tttaactact agagtagcag caaatgctaa
     6601 tattgatgat caaaaagtat tgaatgacta cttgcgtgga cattttttat atatgagcgc
     6661 tgagaacatg aacggaagta ttttagagga atatttagcc gaagtgttag aacctgaagg
     6721 ttggatttgg tgcgccggct ctgtatatcg agctgttgat ttctgttatt taggaacctc
     6781 accaattctt ttacaagtga aaaataaata caatacagaa agcagttcga gttcagctat
     6841 aagagtagga acaacaatta gaaaatggaa tcgtttaaat aagtcgacaa aaatttctgg
     6901 tttggatagc cctatcccca attggaaagc attaatagaa atgactgaag ctagtaagga
     6961 actggcagct aagttaactg aaaatagtta tttagcttat attaatgaaa aatccacacg
     7021 agaattatgg acactcgatg actaacaata tccttattag tatctatcca cgatacatgg
     7081 aaaaaattat aaacaaacaa aaaaactttg aatttagaaa tttctatctt acctcagatc
     7141 cttataatat cttttggatt tacgaaacaa tccccaccaa agctttaaag tataaattga
     7201 ttgtaaaaaa tccaattact aaacttaaga aaaatcaaca ctacttttta ggagacgatg
     7261 agtttgaaca attagtttaa aagggaaaat atgcttctat cattacaagc tcgtttggtg
     7321 taagatgggt ataagtcatt tatgttcact ctccttgtat gctttagcgg gtattacaat
     7381 ttgagtgtaa cataaatggc ttttttattt gtctcgctta attatacaaa cggcgaatta
     7441 taaataactc ataaaaaata ttttgacttt caaaatcact tttgctacca gaagttgagg
     7501 cgaagtgttt taagcatagt tgtaccctag tttgaccgtt aaaaataaat aacgatcgct
     7561 agagtgcaac tatgtttgtt tgccctaccg tcagcgtcgg cttggcctct cacatccatt
     7621 accgtcggga ttatctccgt tacataccct tacaaaatac gccctgtaag cattgcatga
     7681 aaccaccgtg catttgcacc gtatttccgc cctagccgtt caaatcggtc gctaccacca
     7741 ttcagaaaca cgtgcggtaa tggaaggttt ggaggaagct atccagacca ctaacgattg
     7801 tccctatcgt cgtacgtttc actcagatca aggttggtcg tatcaaatga aagcttatag
     7861 aaacaaattg aaggagtaca aaatctttca aagcatgtct cggaaaggga actgcttaga
     7921 taattcactt atggagaatt tttttggctt attaaaacaa gagatttttc acgggaaagt
     7981 ttataactgt ttcgaggaat taaaatcagc catcgatagc tatatctatt actacaataa
     8041 tgaacggata aaacagaaat taaactggca aagtcccgtc caatttcgta aaacaaccgt
     8101 tacggttgtg taaaaatatc attttagagc atgaaaaaaa gagtggattt ctccactcca
     8161 aaaaagtcta acttttgggg gtcactacat ttttgatcct ttttatttga ttaattatat
     8221 ccagctggat aataattttg ggggcaacat ttctatctta ctattatctt cctaaagtag
     8281 tagactttct tattgggaaa aattacaaca aaaaggagtg gcttagttga caactccttt
     8341 ttgtttacaa atcaattgga tatctataaa ttgtattttc taacgaattt tatagacatc
     8401 tgatttgtca aattaagcta gacaatatgt ctttatctat gcagctcaaa aatacttcca
     8461 atggtgttcg ataatttaat gattttcttg ggttctgttg cgaagtttga aaatcaaacg
     8521 cgccctctct gaactccaaa tatcttaggc tgtta
//
LOCUS       DAZABD010000093         8407 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000093 DAZABD010000000
VERSION     DAZABD010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.095"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(413..1099)
                     /locus_tag="ACHALG_002557"
     CDS             complement(413..1099)
                     /locus_tag="ACHALG_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFN2587886.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(1235..1429)
                     /locus_tag="ACHALG_002558"
     CDS             complement(1235..1429)
                     /locus_tag="ACHALG_002558"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587887.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(1479..1919)
                     /locus_tag="ACHALG_002559"
     CDS             complement(1479..1919)
                     /locus_tag="ACHALG_002559"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFN2587888.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELEQLFQAYASLDDVNS"
     gene            complement(1923..2768)
                     /locus_tag="ACHALG_002560"
     CDS             complement(1923..2768)
                     /locus_tag="ACHALG_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFN2587889.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVDWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLEKQSKEQWLVIDGSALSLLVANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAVYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            2917..4335
                     /gene="pepV"
                     /locus_tag="ACHALG_002561"
     CDS             2917..4335
                     /gene="pepV"
                     /locus_tag="ACHALG_002561"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HFN2587890.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(4411..5613)
                     /locus_tag="ACHALG_002562"
     CDS             complement(4411..5613)
                     /locus_tag="ACHALG_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587891.1"
                     /translation="MKLFKKIFFAFVLLIASYCVLLTITFFIPQHSIESNAEKSLQMV
                     EKEGMYPSINQGNMLGTRLDNFTDHLMIRKTKADPELNSLENAMSMADYPRYWHGYQL
                     FLRPLLLGMSLGSIRMIYAAILFLLIGLTSYFLTKCSDIYLTTALLISLAIGNAATFF
                     FSMQFSNIWILTLLSVLLFLTKPQLSNTNQQLFLYFFVIGSLANFFDLLTTPVISWGI
                     PVIIIYYVTNKYLMDKRSSLSKQVGSFVFTGIFWGLGYGLTWVAKWILSSIILNKNIV
                     KDAINQILFRTEGNDKYPLHRLEMLKSNIRLMYPKVAILLLLITCLVFLYFAYRKRNS
                     WRPFQLLVLFVLAAVTPYIWYNILANHSQIHYWFTYRTQIITSFAILSAFAFLIPPEK
                     SKDELNYF"
     gene            complement(5600..7387)
                     /locus_tag="ACHALG_002563"
     CDS             complement(5600..7387)
                     /locus_tag="ACHALG_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6020 family protein"
                     /protein_id="HFN2587892.1"
                     /translation="MHKKKENLFPTFFATLFASGFAFFTSYGLGTLNPTVIIGLAMVL
                     LLAFKQYGIELFSIKNKVAAIARLFFSAAFALTLSIGRQIQFQYVSANYTKTTIADGS
                     IFYFLVYFSIFVYSFLFLQSIYLSMPKLETFFTAQNTQAFSSRKYFLFFTVFLVLCWL
                     PYLLALYPGVVLPDSLSSVAQSMGDVPISNHHPVLFTLLVKLFVHDLPTDTINHGVFL
                     FSIFQTLITASAISYSLCWLLKRNIHFFPVLISLCYFAFAPVFPIYALNMQKDTLFSI
                     ACFLFSLAVFDFIFQREKPTILNQIYVIILALLTTYLRNNGIYVVTGTGLICGLIILK
                     QKRYQAFFQVVLGYTVIAGLLIHPLMARYAIPTETAEQLGVPLQQISRTIVMDGDLSK
                     KDKAFMNQLLPIKDYEAYSPSLADSIKWHKNFNNEFLNTHKKEFLSLWARNLPSNIKI
                     YTDAYILETFGFWVPGTKNSYGFLDTRVNTNNYGMKQVDLIEHLTGLSGMKKIIDARD
                     FFGSGTLLWLAIGSALLAIFKKKYRYLLLFLPGMLVFATIMIATPVAFSLRYVFVLAL
                     GLPVYILSPFLLDKREKMKTDIGDTHETI"
     gene            complement(7400..8353)
                     /locus_tag="ACHALG_002564"
     CDS             complement(7400..8353)
                     /locus_tag="ACHALG_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFN2587893.1"
                     /translation="MESREKIAVLIPCYNEEATISTVIEDFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAAHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVKNTITYLYKTKIRDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMCKDYKPLLFFGIWTIFFFLFGLVMGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYQNKEMK"
BASE COUNT         2758 a         1576 c         1569 g         2504 t
ORIGIN      
        1 aataaagaga ctgtgacaaa agtcgtgtca cagtcctttt tttccgaata aacggtgttc
       61 tcctgtcaac tcctcggtca caagtcaaaa tattcaacaa acatgagaag ctgttttttg
      121 aaattgttcc ttgtgactcg gagatgaacg gcaggctcac gacctcttgg aaaaaggtgt
      181 tcaaaagctt gcatctgcgg taataagaac agccaagtta aaaacataag aagctatttt
      241 tacttggctg ttcttattac ttgatgcagg gcagcttttt cacaacctct caataaacgg
      301 tgtgcaatca gctgaccctc ggtattagct caaaaaaaac ggcaaaatat gaggaactat
      361 tttgccgttt atttctctaa tactcaggtc aaaacgctga ttccaaaacc tcttatttga
      421 tgggtctgtc ctgaatatct tcaaaataat cggggtaatt atgaatcacg tattcttgct
      481 cctcgataat catatgtaaa tgcatcgtgc aaagaaatcc gacttcatca aagttatgat
      541 caatcattga ttgaagcatt gtctgatgtt catctaatac tcgaccccag tctaattctg
      601 aaatcgtcaa acgtaaccat ctgaaacggt tcaagtgcgt actataactt tctagccaat
      661 cccaaatttc tcgttttcca gcaatttcat aacaagttcg gtgaaaatca gtatccaaat
      721 caaaaaaagc ttttttattt tgagcgatca ctgcctgatc ggtttgttct agtatttttt
      781 ctagtttagc ttgatcttgc ttggtcattt tcgcactgca ttcttgcagg atcggttttt
      841 ccaacttttc ccgcgtataa cgggctaatg aagcagatcg gatatcgatt tttgaaacat
      901 aagtgccgga ctgtggaatg gtttttacaa gctcttgatt gcgcagttgg atcagtgctt
      961 ctcgaatcgg tgtacgcccg atagctagtt gttcttctaa cactttatcg gagattttac
     1021 ttccaggtgc taactcggaa taaatgattt gtttacgtat agattcataa gcttgctgtt
     1081 gcaaattatt gataaccatt taaccttcct ttctatgcag atataatcta ttataacata
     1141 aatcgcttac aaatcgtgtg ggcataaaaa cacagtctgc aaaaaaagcc ctgataggca
     1201 tgcctattag agcctcgaaa agaaaaatat tgaatcaatc tggtcgtacc tcttggatcg
     1261 gtttgacacg accgccgatt cttttagcaa aagcttcgga ttcttttttg tctttgaaaa
     1321 tgagcagatt gtctaagttt ttttcccgag tgcgacagcc agttttatcg atgtaaccgt
     1381 gcattacttt aacgacatac atggggatca cctgcttttt tcttttcatt atacgaacta
     1441 ctcgacacaa tacaagaaaa atgcttagac ggtctttatt aagaatttac atcatccaaa
     1501 gaagcgtacg cttggaataa ctgttctaac tccggattga ctttgctcag cttcactaat
     1561 cgttgcgaat cagaaagtaa aaaacagtgt ttactactgc cagtagtttt taattttttg
     1621 tcttcagttc ccacaatttc gtagctaaaa tccaaccgtg ttcccgtata cttttggata
     1681 gttgggtgga tgctcacttt ttctccgtaa tggatcattt ctttatagct gcaatttact
     1741 tccagaacgg ggatgatgat cccagattct tcgatttttt tatagggaaa ccctagatgt
     1801 accaataagg ctacccgagc ctcttcaaac cagcggatat aattgctgtg atgaataatc
     1861 cccatttgat ctgtttcata ataaaaaggt tctcgcacat aacctgtaaa tttctccatg
     1921 actcatgcct cgttttccat tgatttttta caaaaataat tcatccactt cggaagtgct
     1981 tcgctgattt gtgtcggcaa aacaatatat tgttcttttg ccagctggtc tccaatgaaa
     2041 ctatgaagat aaacagctgc tgcaatcgtt tggtcatttt gaggaaactg ggctaaaaat
     2101 cctgtgatca tcccagctaa agtgtcaccc atgccgcctg ttgccatagc agcagaacct
     2161 aaaggattgg tgtagaagtg ctggtgacca taaatttttg tctcatgact tttcaaaacg
     2221 agcacagcac cgatttcctg ttgcttcttt ttgttttctt tatcccgttg ttcttgtatc
     2281 ggtagagaac tcaaccgttg ccactccatt tgatgaggtg taagtacagt ttgttcggga
     2341 tagggtagca aaagatgatt agcaacaagt aaacttaaag cagagccatc aatcaccagc
     2401 cattgttctt ttgattgttt ttctagtact tgtttgaaac gttccaaact cttagaagaa
     2461 gtacccaatc cgggaccaat caagagtaca tctgcttgct ttattacgga aagaaactgg
     2521 tcagtatccg tccaatctac aatcattgct tctggcagat gactgtgaag agcactccga
     2581 tttttttcat cagttactac agtcgtgaga cccgctccac tgtgtacggc agctaaagca
     2641 ctcataatga ttgctccgcc atattgttca ttgccgccaa tcaaaacgat acgtccaaat
     2701 gttcctttat gtgaaatctt tggacgaggt gtgatgactc ttttcactag ttgttcgttt
     2761 atcagcatcg aaaaacctcc ttttactcat tatagcgtat atttttttta gagaaaaaca
     2821 gataagaaaa ggaattgtca gattttttga actatttttt tctgtaattt gtggtatcat
     2881 gaacacgaag acttaaataa gtggaggatt aactttatga caattgattg gcaaaaagaa
     2941 gtagaagcac gtaaagaaga cttgcttgaa gacttgaaaa atcttttacg tgtcaacagc
     3001 gagcgcgatg attcaaaagt aacaccagat gcaccatttg gacctggacc tcgcgacgca
     3061 ttgaaacata tgttagctta tggcgaacgt gatggattta aagtaaaaaa tgtcgataac
     3121 tatgcagggc atattgatct tggcgaagga gacgaaacgc tagggatatt tggacacatg
     3181 gatgtcgttc ctgcaggaga tggctgggac acagatcctt atgaaccagt aatcaaggat
     3241 ggaaagatct ttgcacgcgg atcaagcgat gacaaaggtc caagtatggc agcttactat
     3301 gcgatgaaga tcattaaaga acttgacttg aaactatcaa aaaaagttcg cttcgtagtc
     3361 ggcagcgatg aagaaagcgg ctggggcgat atggcttatt atttcgaaca tgaagaagaa
     3421 ccagattttg gtttctcacc agatgctgaa ttcccaatca tcaatggtga aaaaggaaat
     3481 gtttctttag ctttacgctt caaaggcgac aatgcaggag attatgtctt gaaatctttt
     3541 gtttccggac ttcgcgaaaa tatggtgcca ggaacagcaa ctgcagcact tcaagtacca
     3601 agtgcagatg cagctatcgc gatggaagaa gcattttacc aattcatcga agcaaatcca
     3661 gtaagcggaa cgattgaagc ggacaatacg tatgtaaaaa tcgaattagt tggtaaaggc
     3721 gcacatggtg caagcccaca atctggtatc aatgcaggtt ctttcttggc attgttctta
     3781 gataactatg aattcctagg atctgctaaa caatttatcc atgtagcagc tgcttatgtc
     3841 catgaagatt tttatggaga aaaactagga gtcgcttatg aagatgaaaa aatgggcaaa
     3901 ttgacaatga atgctggact atttgctttt gaagaaaatg gtacagagga agcgaacttc
     3961 atcaatatga atttccgttt tccaaaaggt gtaacagtag acggattgca aagtgacatc
     4021 gaacaaacag tggggcaaga aggcgcaaca gttactcgag gtgctcgcgt aatggaacca
     4081 cattacgtac caatggatga tccattggtg gctacattat tgcaagtcta tgaagaccac
     4141 actggagaaa aaggctacga acaaatcatc ggtggcggaa cttacggacg tttgttgaaa
     4201 cgtggcgttg cttatggagc gatgttccca ggatatactg atacaatgca tcaagcaaac
     4261 gaatttatga gtcttgatga cttgttcaga gcaacagcaa tctatgcaga tgccatctat
     4321 cgtttggcga aataaaaata ctaaaaaact ccggctatct ctgacagctg gagtttttat
     4381 gtttaaaaat gaattagtac atcggtattc ttaaaagtaa tttagttcgt cttttgattt
     4441 ctcaggaggg atcaaaaagg cgaaagcact aagtatagca aaacttgtga tgatttgcgt
     4501 acgatacgta aaccaataat gaatctgtga gtgattagct aaaatattgt accaaatata
     4561 cggcgttaca gcagctaaaa caaacaaaac tagaagttgg aacggacgcc agctatttcg
     4621 tttacgataa gcaaaataga gaaaaacaag acaagtaatc aatagcaata atattgctac
     4681 ttttggatac atcaatctaa tattgctttt cagcatctct aatcgatgca acgggtattt
     4741 gtcgtttcct tccgttcgaa agaggatttg gttgattgca tcttttacaa tgtttttgtt
     4801 caatatgatc gatgatagga tccatttagc cacccatgtc aaaccatagc ctaatcccca
     4861 aaagataccg gtaaaaacaa aactgcctac ttgtttgctc aatgaactgc gtttatccat
     4921 taaatactta tttgttacat aataaataat gatcacaggt atcccccaag agatgaccgg
     4981 tgttgtcaac aagtcaaaaa agttagctaa agaaccaatg acaaagaaat acaagaataa
     5041 ttgttggttg gtgttggaga gttgtggttt tgttaaaaat agtaatacag acaataaagt
     5101 caaaatccaa atatttgaaa attgcatact gaagaaaaag gttgcagcat ttcctatagc
     5161 aagcgaaatc aacaaggctg tcgttaaata gatgtcgcta cacttggtta aaaagtagct
     5221 cgtcaatcca atcagcagaa aaagaatggc tgcgtaaatc attcgaatac tccccaaact
     5281 catccctagc agtaaaggtc gtaaaaaaag ctgataacca tgccaataac ggggataatc
     5341 cgccatagac atcgcgttct ccaaggagtt caattcagga tctgcttttg tttttcgtat
     5401 catcaaatga tcagtaaaat tatctaaccg agtgcctagc atatttcctt gattgataga
     5461 aggatacatt ccttcttttt caaccatctg tagagatttt tctgcattcg attcaataga
     5521 gtgctgcgga atgaaaaaag taattgtcag tagtacgcaa tagctagcga tcaagagaac
     5581 aaaagcaaaa aatatctttt taaatagttt catgagtatc tcctatatct gttttcattt
     5641 tttctctttt atctagtaaa aaaggagaga ggatataaac aggtagacct aatgctaaaa
     5701 caaacacata ccgtaaacta aaagccacag gagtagcaat cataatagta gcaaaaacaa
     5761 gcataccggg aagaaaaagg agtaaatacc gatatttttt cttaaagatt gctaaaagtg
     5821 cactgccgat agctaaccac agtaatgtgc cggaaccaaa gaaatcacga gcatcgatga
     5881 tttttttcat tcctgacaat ccagtaagat gttcaatcaa atctacttgc ttcatcccat
     5941 aattattcgt gtttacacga gtatctaaaa agccgtaact attttttgta ccaggtaccc
     6001 agaacccgaa cgtttctaaa atataagcat ctgtatagat ttttatatta cttggtaaat
     6061 ttcttgccca tagtgataga aattcttttt tatgtgtatt caaaaactca ttgttaaaat
     6121 ttttatgcca cttgattgag tctgcaagac taggagaata cgcttcataa tcttttatcg
     6181 ggagtagctg attcatgaat gctttatctt tcttagacaa gtcaccatcc ataacaatag
     6241 ttctagaaat ttgctgcaaa ggaacaccta attgttcagc agtttctgta ggaatagcat
     6301 aacgagccat caacgggtgg atgagtagtc cagcaatcac tgtataaccc agcaccactt
     6361 gaaagaatgc ttgatacctt ttttgtttca aaataatcaa gccgcaaatc aaacctgtgc
     6421 cggttactac ataaatccca ttatttctta gataggtggt cagtaaagca aggatgatta
     6481 cgtaaatctg attgagaatc gttggctttt ctcgttgaaa aataaagtca aaaaccgcta
     6541 aggaaaataa aaagcaagcg atagagaaca aagtatcttt ttgcatgttc aaggcgtaga
     6601 ttggaaaaac cggtgcaaat gcaaaatagc ataaactaat gagtaccgga aaaaaatgga
     6661 tgtttctttt taacagccag caaagagaat aacttatcgc tgaagctgtt atcaatgttt
     6721 gaaaaatcga aaataaaaat acaccgtgat tgatcgtatc agttggtaaa tcatgaacaa
     6781 atagcttgac cagtaaggtg aataagactg gatgatgatt gcttatagga acatcaccca
     6841 tagattgggc gaccgaactt aacgaatcag gcaaaaccac tccaggatac aatgccaaca
     6901 gataaggaag ccaacacaga actaaaaaga cagtaaagaa taaaaaatat tttcttgaag
     6961 agaaagcctg tgtgttttga gcagtaaaaa atgtttccag ttttggcatt gataaataaa
     7021 tcgattgtaa aaataaaaaa gaatacacaa aaatggagaa ataaacaaga aagtaaaaaa
     7081 tacttccatc agcaatcgtt gtctttgtat agtttgctga gacatactga aattgtatct
     7141 gtctgccaat agaaagtgta agagcaaaag ctgcagaaaa aaacaatcgg gcaatagctg
     7201 caactttgtt ttttattgaa aacaactcta ttccatattg cttaaaagca agtagcaaaa
     7261 ccatggctaa accaatgatt actgttggat tcaaagttcc tagaccataa gaagtaaaaa
     7321 aagcaaaacc tgacgcaaat aaagttgcaa aaaaagtagg aaatagattc tcctttttct
     7381 tatgcataaa aaaggcctcc tacttcattt ctttattttg atattcgtgg tagacatggt
     7441 ataaattcaa ttcatattct ttcttggcat ttgtgacgac cgtatccaaa atcaaccctg
     7501 ttactaatga aagaagggct aaagtcatca atccagtact taaaatcgca gaagggatgc
     7561 gtgtaataaa gcttgtcagc atatattcac gaatcacagg aatccccatg accaatccaa
     7621 ataaaaagaa gaagatggtc cagatcccaa agaaaagcaa aggtttataa tctttgcaca
     7681 ttttgatgat catcatgatc actttgaatc cgtcagagaa tgtattcaat tttgattcgc
     7741 taccttcagg acgatcgcga taatcgattg gaatctcaac gaacttgaat tttttatcta
     7801 aagcatgaat cgtcaattct gtttcgattt gaaaaccaga actcataata gggaagcttt
     7861 tgacaaaaat tcgattgaat cctcgatagc cggtcatgac atcgcgaatt tttgttttat
     7921 ataagtaagt aattgtattt ttaaccagat tattcccaaa atcatggaaa ggacgcttat
     7981 tttcatcaaa ataggttcca tttgatagac gatcgccaat gaccatatct gcttcgccag
     8041 aacgtaattt ctcaagcaaa ccatgtgcag cttctgcagg ataagtatca tcaccatcta
     8101 ccatcaaata ataatcggca tcaatatcaa aaaacatttg acggattaca tttccttttc
     8161 cttgacgtgg ttctttcttt acgattgccc caccttgaac ggccaattcg tatgttcgat
     8221 cggtcgaatt attatcatag acataaatat ctgcttctgg taattccctt ttaaaatctt
     8281 caataacagt tgaaattgtc gcctcttcgt tataacaagg aatgagcacg gcaatttttt
     8341 cacgtgattc catttgtcac tctccttata taataaaact aatcttattt caagaatacc
     8401 atataat
//
LOCUS       DAZABD010000094         8401 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000094 DAZABD010000000
VERSION     DAZABD010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.096"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..996
                     /gene="lacD"
                     /locus_tag="ACHALG_002565"
     CDS             4..996
                     /gene="lacD"
                     /locus_tag="ACHALG_002565"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFN2587894.1"
                     /translation="MHKLTAGKKAAMDRLSTKEGVISALAIDQRGALKKMIKALGVEP
                     TIADIEQFKELVSRELTPYASSILLDPEYGLSAAKSRHENAGLLLAYEKTGYDVTVPG
                     RLPDLLTDWSVMRLKKQGADAIKFLLYYDIDEDSKINQQKHVFVERLGSECAAEDIPF
                     YLELVSYDAQNIDVNSFEYAKIKPHKVNRMMEEFSKEQYKVDVLKVEVPVNMNYVEGF
                     TMDKVVYTKEEAANYFLEQSQATDLPFIFLSAGVSAELFQQTLVFAKESGSTFNGVLC
                     GRATWKNGVQPFVESREKGACEWLQTQGRSNIESLNKVIAETASSWHKKIKVES"
     gene            1011..1946
                     /locus_tag="ACHALG_002566"
     CDS             1011..1946
                     /locus_tag="ACHALG_002566"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014293854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HFN2587895.1"
                     /translation="MITTVTMNPSVDISYSLDYLNIDKVNRANQVTKTAGGKGLNVAR
                     VLHDLQGDVLVTGMLGGTHGTFITRELDKAGIKHDFVTIKGETRDSIAILHEGNQTEI
                     LESGPTISIDEQKLFLDKFRKLVGLAKIITISGSLPEGIPTNFYQQLVKIAKDNGVKI
                     LLDTSGISLRKALIGNNKPYMIKPNLSELEALLGKTFSLEYLDEIQKALFDSLFKGIE
                     WIVVSLGKAGAIAKHGETFYKVTIPKIKVFNPVGSGDASIAGFAYAMTKNMNANDMLK
                     MCMATGMANAQERITGHVDLSNVEKHFSRIDVKLI"
     gene            complement(1965..2675)
                     /locus_tag="ACHALG_002567"
     CDS             complement(1965..2675)
                     /locus_tag="ACHALG_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016181193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFN2587896.1"
                     /translation="MTLPKYQLIKNNLKQQITSGAFENGDKFYTESELTELFKVSSIT
                     VIRALNDLEKEGFLVREQGRGTFVSRARKEKLVHFSDIELFPIRNDCVEVLSIKHGNQ
                     SQFLEKLNLPSNASYYCIERIRKNKNTPYIYHETYLPEQYVNPHYPALSYYNSIYQRF
                     KLDYDIHMNEEDFTETNEIRFPTPNEKAKKLQISPNEPTIFQIKVTKRKDTQQILEYV
                     ESYKKWDFYKIKLTSENA"
     gene            2846..4624
                     /locus_tag="ACHALG_002568"
     CDS             2846..4624
                     /locus_tag="ACHALG_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase family protein"
                     /protein_id="HFN2587897.1"
                     /translation="MTTFEIKEEFLLNGKTFKILSGAIHYFRIPPCDWEHSLYNLKAL
                     GFNTVETYVPWNLHEPQKGEFHFEGILDLERFLTIAQDLGLYAIVRPSPYICAEWEFG
                     GFPSWLLREPIHIRRNETAYLEHVADYYDVLMKRIVPHQLDNGGNILMIQIENEYGSF
                     GEEKEYLRAIRDLMSERGVTVPFFTSDGPWRATLRAGSMIEDDILVTGNFGSKAKDNF
                     DSMKQFFKEYDKNWPLMCMEFWDGWFNRWKEPIIQRDPQELAEAVKEVLEQGSINLYM
                     FHGGTNFGFMNGCSARGVIDLPQITSYDYGAPLDEQGNPTEKYYALRKMIHDNYPEIK
                     QLDPVIKPTIEKKKISLTNKVSLFATLDTISQPIQSKYPKTMEELGQNTGYLLYRTMI
                     ERDAEEERLRVIDGRDRSQLFLNQKLQATQYQTEIGEDIQVSMTEETNQVDILIENMG
                     RVNYGHKLFAETQKKGIRTGVMADLHFITNWQQYSLPLENCKTVDYSKGWQPQHPSFY
                     RYEFLLEDVGDCFIDLSKFGKGIVFVNQTNIGRFWEVGPTLSLYISSGFLKVGKNEIV
                     IFETEGTYEPEINLLKAPLYKEMKEG"
     gene            4628..5107
                     /locus_tag="ACHALG_002569"
     CDS             4628..5107
                     /locus_tag="ACHALG_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFN2587898.1"
                     /translation="MSIIGVRIDGRLIHGQVANLWTTKLNISRIMVVDDEVAQNDIEK
                     SGLKLATPAGVKLSVLPIEKAAQNILAGKYDSQRLLIIARKPDRLLKLVELGVPIKEI
                     NVGNMSQTSETRSITKSINVVDQDVETFNQLNAKGVHLIAQMVPSDKADDFMSLLNK"
     gene            5131..6033
                     /locus_tag="ACHALG_002570"
     CDS             5131..6033
                     /locus_tag="ACHALG_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFN2587899.1"
                     /translation="MDILWWQVLLLSLYAGYQILDELQIYSSMNAPVFAGFFSGLVMG
                     DVVTGLYIGGSLQLMVLGVGTFGGASKIDANSGTILATAFSVALGMDPEQAVAAIAVP
                     VASLMIQLDVLARFANTFFAHRIDKNVEDMNYKAIGRNFLLGALPWSLSRLVPVFLAL
                     AFGGGVVEKVVAVLNGDLKWLGDGLSVAGAVLPAVGFAILLRYLPVKKHFPYLILGFT
                     ITALLTTVFSNIQLLGTSVASVVKDFQGVFNGLPMLGIALIGFAFAAISYKNSSNKTV
                     IQSQEDTKSESKTLDGGEIDDDEI"
     gene            6020..6829
                     /locus_tag="ACHALG_002571"
     CDS             6020..6829
                     /locus_tag="ACHALG_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFN2587900.1"
                     /translation="MTKSNYQLTKKDFKQINRRSLFNFQLGWNYERMQGSGYLYTILP
                     QLRKIYGDNTPELKQAMKTHTQFFNTSNFFNTIITGIDLAIEEKEGIKAIDTVSGLKA
                     GLMGPFAAIGDSIFGALIPTIFGALAANMAINGNPTGIFIWIVAQMAVNVFRWKQLEF
                     AYKEGISLVTTMQHRLTALTDAATLMGVFMVGALAATMVNVKFAWAPSIGDVTLNMQN
                     NLDMILPRLLPAGIVGAIYWMLGKKNMTSTKAILIVLVVCVALSALGVISK"
     gene            6845..7249
                     /locus_tag="ACHALG_002572"
     CDS             6845..7249
                     /locus_tag="ACHALG_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFN2587901.1"
                     /translation="MKHNLVLISHGNFCEELKNSAEMIMGSQNNIYTVPLLPSEGPED
                     FKKKFFDIVQGLDEYIVFADLMGGTPCNIISKEIISGGNIPLYAGMNLPMVISFINGE
                     LINVESDFVVDGKNSICDVGKVIVEDLKFEDE"
     gene            7548..7778
                     /locus_tag="ACHALG_002573"
     CDS             7548..7778
                     /locus_tag="ACHALG_002573"
                     /inference="COORDINATES: protein motif:HMM:TIGR01168.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HFN2587902.1"
                     /translation="MYIKNQKQCKYAIRKTTLGVGSVLIGVMLFGTNISAEAAENTSS
                     IASTSNIRERSIESPSSVPVEEISTETVEDQS"
     gene            7778..8287
                     /locus_tag="ACHALG_002574"
     CDS             7778..8287
                     /locus_tag="ACHALG_002574"
                     /inference="COORDINATES: protein motif:HMM:NF012956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HFN2587903.1"
                     /translation="MLENQFVGKKFEIKEGKLSSVAIENKLTDEMLNLSDSKEFVIRF
                     KEDSNGWGFSDDRNEWNLFASSEANLLNEGAINLATDGDLNTIYHSNYGQGTGTVNKL
                     SVTIEFAFPDKKDLKTFIYVLRQNGTNGNIKEFKISIKEDNNEVYKEIKHVHFINDKS
                     SLYMGEKGE"
     gene            8293..>8401
                     /locus_tag="ACHALG_002575"
     CDS             8293..>8401
                     /locus_tag="ACHALG_002575"
                     /inference="COORDINATES: protein motif:HMM:NF040237.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KxYKxGKxW signal peptide domain-containing
                     protein"
                     /protein_id="HFN2587904.1"
                     /translation="MEVKKRFKMYKKGKTWVIAPILFIGVASFTVNNEVA"
BASE COUNT         2850 a         1069 c         1759 g         2723 t
ORIGIN      
        1 ataatgcata aattaacagc tgggaaaaaa gcagcaatgg atcgattgtc aacgaaagaa
       61 ggagttatta gtgctttagc aatagatcaa cgaggggctc taaaaaaaat gataaaagca
      121 cttggagtag agccaacaat tgcagatata gaacaattta aggaattagt ttctagggaa
      181 ttgacgccgt atgcgtcatc aattttgtta gatcctgaat atggattatc tgcagcaaaa
      241 tctcgacatg agaatgcggg ccttttactt gcgtatgaaa aaactggata tgatgtaacc
      301 gttcctggac gcttaccaga tttattaaca gattggtctg tcatgcgttt gaaaaagcaa
      361 ggtgcagatg caattaagtt tttgctttat tatgatattg atgaggattc gaaaatcaat
      421 caacaaaaac atgtttttgt tgaacgttta ggtagtgaat gtgcggcaga agatatacct
      481 ttttacttag aactcgtttc ttatgatgct caaaatattg atgtaaattc atttgaatat
      541 gcaaaaatta agccacataa agtcaataga atgatggagg aattttcaaa agaacaatac
      601 aaagtagatg tattgaaagt agaagttcca gtcaatatga attatgttga aggatttact
      661 atggataagg tagtttatac caaagaggaa gcagcaaatt acttcttaga gcaaagtcag
      721 gcgacagatt tgccatttat tttcttaagt gctggtgttt cagctgaatt attccaacaa
      781 actttagttt ttgcaaagga atcagggtca actttcaacg gtgttctttg tggccgtgca
      841 acatggaaaa atggagttca gccatttgtt gaatctagag aaaagggtgc ttgtgaatgg
      901 ttgcaaactc agggacgttc caatatcgag agtttaaata aagtgattgc agaaacagct
      961 agttcttggc ataaaaaaat aaaagttgaa tcctgaaaga aggtaatttc gtgataacaa
     1021 cggtaacgat gaatccctct gttgatattt cttattcatt agattactta aatattgata
     1081 aggttaatcg agcgaatcaa gttacaaaaa cagcaggagg aaaaggactg aatgttgctc
     1141 gtgtactcca tgatttgcaa ggagatgttt tagttacagg gatgctagga ggaactcatg
     1201 ggacttttat tactagagag ttagacaaag ctggaataaa acatgatttt gttacaatca
     1261 aaggagagac acgagactct attgctattt tacatgaagg gaatcaaacc gaaattttag
     1321 agtctggacc tacaatttct attgatgaac aaaagttgtt tttagataaa ttccgtaagt
     1381 tagtagggct ggctaagatt attacaattt ctggtagttt gccagagggg atacctacta
     1441 atttttatca acaattagta aaaattgcaa aagataacgg agtgaaaatt ttgcttgata
     1501 cctctggtat aagtttaaga aaagctttaa tagggaacaa taagccgtac atgattaaac
     1561 ctaatttgtc agaattagag gccttgttag gaaaaacttt ttcattggaa tacttggatg
     1621 aaattcaaaa agctttattc gattccttgt ttaaagggat cgaatggatc gttgtttctt
     1681 tagggaaagc aggtgcaatt gctaagcacg gtgaaacgtt ttataaggta actattccta
     1741 aaataaaagt attcaatcct gttgggtctg gcgacgcttc aattgcaggt tttgcttatg
     1801 caatgactaa aaacatgaat gctaacgaca tgttaaaaat gtgtatggct acgggtatgg
     1861 caaatgctca agaacgtatt acaggacatg ttgatttatc gaatgtagaa aagcattttt
     1921 ctaggattga cgtgaaacta atctagctat tattagtgtt ttagttaagc attttctgaa
     1981 gttagtttta ttttataaaa atcccatttt ttataggatt caacatattc taaaatttgt
     2041 tgagtatctt tacgtttagt cactttaatt tgaaagatag ttggttcgtt tggcgatatt
     2101 tgtaattttt ttgctttttc atttggagtt gggaatctta tctcattagt ctcggtaaaa
     2161 tcttcctcat tcatatggat atcataatct agtttgaaac gttgatatat agaattataa
     2221 taacttaatg ctggataatg aggatttacg tattgttccg gtaagtacgt ttcatgataa
     2281 atatagggtg tgtttttatt ctttcgtatt cgttcaatac agtaatagga ggcattggaa
     2341 ggtaagttta atttttctaa gaattgtgac tgatttccat gtttgatgga taaaacctct
     2401 acgcaatcat ttctaatagg aaaaagttca atgtcagaaa agtgaacgag tttttctttg
     2461 cgtgctcgtg aaacaaacgt tccacgtcct tgctcacgta cgagaaatcc ttctttttct
     2521 aaatcattta atgcacgaat gacagtaatt gaactaactt tgaatagttc ggttaattca
     2581 gattctgtat aaaatttgtc accattttcg aaagcgccgg aagttatttg ttgctttagg
     2641 ttgtttttaa tgagttgata ttttggcaag gtcataactt agtcctccca ctaactagaa
     2701 taaaataaaa gtgaacgtta atcatgtaca tttgatttta ccacataaaa cataaataaa
     2761 aagcattgat tttttattta aaaggattta atatattaat ataaaaagtt gggtgcatcc
     2821 tattaattga gtaaggagtg agaaattgac tacatttgaa ataaaagaag aatttttatt
     2881 aaatggaaaa acatttaaaa tactgtctgg tgcaattcat tattttcgga ttcccccgtg
     2941 tgattgggaa cattctttgt ataatttaaa agcactagga tttaatacag tagagacata
     3001 tgttccttgg aatttacatg agcctcaaaa aggagagttt cattttgaag ggatattgga
     3061 tttagagaga tttttgacca tcgctcaaga cttaggatta tatgctattg tgcgaccatc
     3121 gccttatatt tgtgctgaat gggaatttgg agggtttccg tcatggctac tccgtgaacc
     3181 gattcatatc cgaaggaatg aaaccgctta tttggaacat gtagcagatt attatgatgt
     3241 tttgatgaaa aggattgttc cccatcaact tgataatggg ggaaatattt tgatgattca
     3301 aattgaaaat gagtatggat cttttgggga agaaaaggaa tatttgcgtg cgataagaga
     3361 tttgatgagt gagcgaggag tgacagttcc ttttttcact tcagatggac cgtggagggc
     3421 tactttacga gcgggaagca tgattgaaga tgatattttg gttactggta attttggttc
     3481 taaagcaaaa gacaactttg actctatgaa gcagtttttt aaagaatatg ataaaaattg
     3541 gccactgatg tgtatggaat tttgggatgg ttggtttaat cgttggaaag aaccaatcat
     3601 tcaacgagat ccacaggaac tcgctgaagc ggtaaaagag gtattagaac aaggaagcat
     3661 taatttatat atgtttcatg gaggaacgaa ttttgggttt atgaatggtt gttcagcaag
     3721 aggagtgatt gatttaccgc aaattacttc ttatgattat ggcgcaccat tagatgaaca
     3781 ggggaatcca acagaaaaat attatgcatt gcgtaaaatg attcatgaca attatccaga
     3841 gattaagcag ttagatcctg tcattaaacc cacgattgaa aagaaaaaga tttctttgac
     3901 gaataaagta agtcttttcg cgactttaga taccattagt caaccgattc aatcaaagta
     3961 tccaaagaca atggaagagt tgggacaaaa tacaggttat ctactttacc gaacaatgat
     4021 agagagggat gcagaggaag aacgattacg ggtaattgat ggcagagatc gcagtcaatt
     4081 atttttgaat caaaaattac aagctaccca atatcaaaca gaaataggtg aagatataca
     4141 agttagtatg acagaagaaa ccaatcaagt ggatattttg attgaaaata tgggacgtgt
     4201 caattatgga cataaacttt ttgcggaaac gcaaaaaaaa ggaattagaa caggagtaat
     4261 ggcagactta cattttatta caaattggca acaatattca ttgcctttgg agaattgtaa
     4321 aactgtggat tattcaaaag gatggcagcc ccaacatcca agtttttatc gctatgagtt
     4381 tcttttagag gatgtcggtg attgttttat tgatttaagt aaatttggaa aaggaattgt
     4441 ctttgttaat cagaccaata tcggtcgttt ttgggaagta ggtccaacgc tttcactgta
     4501 catctctagc ggctttctaa aagttggtaa gaatgaaatt gttatttttg agacagaagg
     4561 cacctatgaa ccagaaatca atttattaaa agcaccacta tataaagaaa tgaaagaggg
     4621 atgaacaatg agtattattg gagtaagaat tgatggacgt ttaattcacg gacaggtagc
     4681 caatttatgg acaaccaaat tgaatatttc tcgaattatg gttgtggatg atgaagtggc
     4741 tcaaaatgat attgaaaaaa gcggattgaa attggctacc ccagcagggg tgaaactaag
     4801 cgttttgcca attgaaaaag cagcgcaaaa tattttagca ggaaaatacg attcacagcg
     4861 tttactgatt attgcacgaa agcctgatcg attattaaaa ttagtagaat tgggcgttcc
     4921 aataaaagag attaatgttg gaaatatgtc acaaacgtct gagactcgat caattacgaa
     4981 atcaattaat gttgtcgatc aagatgtaga aacgttcaac caattaaatg caaaaggtgt
     5041 ccatttaatt gcgcaaatgg ttccaagtga taaagcagat gactttatgt ctctgttaaa
     5101 caaataaaaa tatatgtagg aggagaaaaa atggatattt tatggtggca ggtcttatta
     5161 ttatcattgt acgcaggtta tcaaatttta gatgaactgc aaatttattc ttcaatgaat
     5221 gctcctgttt ttgctggctt tttttctggt ttagtaatgg gggatgttgt aacaggacta
     5281 tatattggtg gtagcttaca attaatggta ttaggtgtag gaacttttgg tggagcttca
     5341 aaaattgatg ctaactctgg tacgatttta gctacagcat tttcggtagc tcttggaatg
     5401 gatccagaac aagctgtagc tgcgatagct gttccagtag ctagtttaat gattcaatta
     5461 gatgttttag cacgttttgc taatacattt tttgcacatc gtattgataa aaatgtagaa
     5521 gatatgaatt ataaagcaat tggtcggaat tttcttttag gagcattacc ttggagtcta
     5581 tctagattag ttcctgtgtt cttagctttg gcattcggcg gaggcgttgt ggaaaaagta
     5641 gtggctgttt taaacggaga tttaaaatgg ttaggagacg gattatctgt agcaggagct
     5701 gttttaccag ctgtagggtt tgcaatttta ttgagatatt taccagtcaa aaaacatttt
     5761 ccttatttaa tcttaggttt tactataact gcattattga ctacggtgtt tagcaatatt
     5821 cagttgcttg gcactagtgt agcaagtgtt gtgaaagatt ttcaaggtgt atttaatgga
     5881 ttgccaatgt tgggcattgc tttaattggt tttgcttttg ctgcaattag ttataagaat
     5941 agttctaata aaaccgttat tcagagtcaa gaggatacta aatctgaaag taaaacttta
     6001 gacggagggg aaattgacga tgacgaaatc taattatcaa ttaactaaaa aggattttaa
     6061 acaaattaat agaagaagtt tattcaattt tcaattgggt tggaattatg aacgaatgca
     6121 aggctctggc tatttgtata ctattcttcc acagttacgg aaaatttatg gggataatac
     6181 gccagaatta aagcaagcga tgaagactca tacacagttt ttcaatacat ctaatttttt
     6241 taatacgatc attacaggga ttgatttggc tattgaagaa aaagaaggaa ttaaggcaat
     6301 cgatactgta tcaggtttga aagctgggtt gatgggaccg tttgctgcta ttggggattc
     6361 aatttttggt gcgttaattc ctacgatatt tggagcgtta gctgcaaata tggcaattaa
     6421 tggtaatcca actggaattt ttatctggat tgttgctcaa atggcagtta atgtttttcg
     6481 atggaaacag ttggaatttg cttacaaaga aggaatatca ttagttacta caatgcagca
     6541 tcgcttaaca gctttaacag atgcagcaac attaatgggt gtatttatgg ttggagctct
     6601 agctgctact atggttaatg taaagtttgc ttgggcacca agtataggtg atgttacttt
     6661 aaatatgcag aataatttag atatgatttt accaagattg ttaccagcag gaattgttgg
     6721 agcgatttat tggatgttag gcaaaaagaa tatgacttct acaaaagcaa ttttaattgt
     6781 attggtagtt tgtgttgctt tatctgcttt aggagttata tctaaataaa caggagtaaa
     6841 tagaatgaaa cataatttag tattaataag tcacgggaat ttttgtgaag aattgaaaaa
     6901 tagcgctgag atgattatgg gatctcagaa taatatttat acagtccctc tattaccttc
     6961 agaaggccca gaagatttca aaaaaaaatt ttttgatatt gtacaaggat tagatgaata
     7021 tatagttttt gcggatttaa tgggaggaac accttgtaat attatttcaa aagagattat
     7081 ttctggggga aatattccgc tgtatgcagg aatgaatcta ccgatggtga tcagttttat
     7141 taatggagag ctcattaatg ttgaaagtga ttttgtagtt gatggaaaaa atagtatttg
     7201 tgatgtaggt aaagtaatcg ttgaagattt gaaatttgaa gatgagtagg atgtgtaatt
     7261 agaatcctta tcttttgaaa aatcttaaaa gttactttct ttaaaagata aggttatcgt
     7321 cagatttagt agttgacgtt aatcttatct ttttttgctc tttgtctcgt atcctcacac
     7381 taaattacca attaaacaaa gagctgtggt tagagcctcg taatactagt tattttgaaa
     7441 gaaatactat tggagggaat actactccac agcgcataca ttgagtataa aataactcaa
     7501 cggaaagcgg gagctgccca taattaataa tgtaaggagg gattataatg tatattaaaa
     7561 atcaaaaaca gtgtaaatat gctatcagaa aaacgacatt aggagtaggt tcagtgttaa
     7621 taggggttat gctttttggt acaaatatat cagctgaggc agcagaaaat acttcaagta
     7681 tagccagtac gagtaatata agagaaagaa gcatagaatc tccttcttca gttccagtag
     7741 aagaaataag tactgaaact gttgaggatc aaagctagtg ttagaaaatc aatttgttgg
     7801 taaaaaattt gaaattaaag aagggaagtt aagtagtgta gccatagaaa acaaattaac
     7861 ggatgaaatg ttgaacttgt ctgattctaa agagtttgtt attagattta aagaagatag
     7921 caacggatgg ggctttagtg atgatagaaa cgagtggaat ttatttgctt catctgaagc
     7981 taatttgtta aatgaaggag caataaatct tgcaacagat ggagatttaa acactattta
     8041 tcatagtaat tatggtcaag ggacaggaac tgtgaataaa ttgtcagtaa ctattgagtt
     8101 tgcttttcct gataaaaaag atttgaagac ttttatttat gtacttaggc aaaatgggac
     8161 aaatggaaat atcaaagaat ttaaaatttc tattaaagag gataataatg aagtgtataa
     8221 agagataaaa catgtgcatt tcattaatga taagtcttca ttatatatgg gagagaaagg
     8281 agagtaattt gtatggaagt aaaaaagcgg tttaaaatgt ataaaaaggg taaaacatgg
     8341 gttatagcac ctattttatt tataggtgta gcgagtttca cagtaaataa tgaagtggct
     8401 a
//
LOCUS       DAZABD010000095         8047 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000095 DAZABD010000000
VERSION     DAZABD010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8047
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.098"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            151..1890
                     /gene="yfcC"
                     /locus_tag="ACHALG_002576"
     CDS             151..1890
                     /gene="yfcC"
                     /locus_tag="ACHALG_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFN2587905.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            2047..3231
                     /gene="iadA"
                     /locus_tag="ACHALG_002577"
     CDS             2047..3231
                     /gene="iadA"
                     /locus_tag="ACHALG_002577"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFN2587906.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3342..3545
                     /locus_tag="ACHALG_002578"
     CDS             3342..3545
                     /locus_tag="ACHALG_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFN2587907.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3646..4308)
                     /locus_tag="ACHALG_002579"
     CDS             complement(3646..4308)
                     /locus_tag="ACHALG_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN2587908.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4321..5823)
                     /locus_tag="ACHALG_002580"
     CDS             complement(4321..5823)
                     /locus_tag="ACHALG_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN2587909.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6371..>8047
                     /locus_tag="ACHALG_002581"
     CDS             6371..>8047
                     /locus_tag="ACHALG_002581"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFN2587910.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
BASE COUNT         2498 a         1428 c         1737 g         2384 t
ORIGIN      
        1 taatcataca taggagaaaa aaaacaaaat agaaaaaagt gattttctaa taaattattc
       61 atgtggaaat gtcattttta ctgtataatt catttgatta tccatttttt attcatcaaa
      121 attactgaaa aaagaaagga agaaataaat ttgaagaaaa caaattcttc aggaaaaccg
      181 aaacgcgact tcactaaatt atcgacaccc catacgtatg tcattatttt tggtgtagtc
      241 atctttgcat ggatactgac ttttgtcgtt ccagcaggaa agttcagcac gcaagatatt
      301 gaatacaaag atgcaaatgg cgaaacaagt actcgtactg ttttacgtca agattccttc
      361 cgttatgctt acgagctgga taagtcgtat gtgtttgatc aattagaaga actgcaagat
      421 catccagcag aacgtgaaaa actggatgtt ccggaaaaag gattggaaaa agtcattgct
      481 gacggagaaa aaaatcttac gcaggaaaaa ctagatgaaa tctccttgac agatgatgtg
      541 ctttacgatc agtacggcga aaatatctat gatacttcta aaaaattgca taaaacagcc
      601 aaaatatggg gaacagatga ttttggcggc tttggtttct taaattttgt ttttgaagga
      661 ctagtatctg gagataagta tggatctgct gtagggatcg ctgcattgat ccttgttgtt
      721 ggaggtgctt ttggtattat tatgcgtacc ggagcgatcg acgcaggtat ctatgcgttt
      781 atcagtaaaa ctaagggact ggagcgttta gctttaccgc tattattctt tgctttttca
      841 ttcggtggcg ctacattcgg gatggcagaa gaggtaattc cattctcaat ggtgatggtt
      901 ccatttgtca ttgcgttagg atatgactcg atcgtagcag taaccgtgac ttatgtcgca
      961 tctcaagtcg gaaatgcgac ctcttggatg agcccgttta gcgtagcggt ggctcaaggg
     1021 attgctggaa tccccgtgtt gtctggtgcg acattccgat tgattatgtg ggtagttgtt
     1081 actgcattag ccgctggtta tatgatgata tatgcagaaa aaatccgtaa aaaaccagaa
     1141 agttcgttga cttacaaatc agatgattat ttccgttctc atatcaaaaa gacatctgat
     1201 gaaaataaaa aattcatgct tgggcataaa ttgatcttat tagagatgtt agttgtcctt
     1261 gtctggatcg tttggggcgt gactcaaaaa ggctattaca ttccagaaat cgcttctcaa
     1321 ttctttgtga tgggattagt tgctggaatc attgctgttc ttttcaaatt agatggaatg
     1381 aaagtcaatg acatcgcttc ttctttccaa tctggtgcag ctgatttagc tggtacagca
     1441 atcgtagtag gtatggccaa agggattctt cttgtcttgg gaggatctga tgcatctgtt
     1501 ccttcagctt tgaatacgat tttgcatggg atcgggacgg cgttgaccgg tgttccagct
     1561 gtcattggtg cttgggcgat gtacatcttc caaagtttgt tcaacttggt tgttacatca
     1621 aattcaggac aagcagcttt gactatgcca atcatggctc cactggcaga tttagtaggt
     1681 gtatccagac aagtagcagt tcttgcttat cagcttggtt caggttttat ggacgctttt
     1741 acacccgtat cagccagctt gattggtgtg ctaggtgttg cacggatcga atgggcaaaa
     1801 tgggcaagat tccaaatcaa gatgcaagga ttcctgtttg ttttaggaac tattttcatc
     1861 atgattgcca ttgcaatagg tctgcagtag tactggagac ctgtaaataa aaaagtaatc
     1921 caaacagtag ataaggactc caagaaccgt cattttacgg taaaatcaaa ggagtaaaga
     1981 tcctatcgtt tggattattt tttaagatcc tatcatcagt tatacttaaa gaaagaggaa
     2041 aaatgtatga aaatcattcg acaaattgaa gtgtttgcac cagattactt aggaaaaatg
     2101 gatgtactga ttgccggtgg gaagattttg gctgtagaag aacagctaga aggcggctac
     2161 gaaggagtgg aagtagaaga actttcaggt gagggaaaag tactaacccc gggtttcatt
     2221 gactgtcatt tccatatttt aggtggagga ggagaaggcg gttatcaaaa tcgaacgcca
     2281 gaagtcacat tgagccaatt gactacagct ggtgtaacga cagttgtcgg ttgtttggga
     2341 acagacggag aaggtagaga tatgactgcg ttgatcagca aagcgaaagg tctggaagca
     2401 gaaggaatct ccacttatgt atacgaaggt tcgtaccgat tgccggtaaa aacagtaaca
     2461 gattctatca tcaaagactt tttgacaatt gataaaatta tcggaatcgg agaaatcgca
     2521 gtatccgatc accgttcttc tcagccaagt tttgaagagt tcgctcgtgc agtagcagat
     2581 gctcgtgtag gaggcatgtt atccggaaaa gctgggatca tcaatgtcca cttaggcgga
     2641 ggaaaaagaa agctagagct attgacacga atcgtagaag aaacagaaat tccgatcaca
     2701 caatttttgc ctactcatgc aaatcgcaca ccagaattat ttgaagcttg cgtggcattt
     2761 gccaaacgtg gtggcacgat tgacttcact gctagcgaag atcctgattt ttgggagaag
     2821 acagatggtg aagttcgctt cagcaaagca ctgaaacgat tgatcgagga agatgtttct
     2881 ttagataatt tcacgatgac ctcagatgga caaggaagct tgccttattt tgatgaaaac
     2941 aatcatttcc tagggttagg agtaggaagt gccaaggcat tgctagtagg gatcaaagaa
     3001 gctgtgcaaa aagaaagtat ccctttggaa atcgccttac gagctataac cagtaatcca
     3061 gcacgtatat tgaaattgga taaaaaagga aaaatcgaaa ttggtgctga tgcagatctg
     3121 tgtatcttgg ataaagaaac gcttgatatt gatacagtta tcgctaaagg agaaataatg
     3181 gtccaagaaa aagaagtcaa agtatggggg actttcgaaa aaagttttta atcaacagct
     3241 ctaactatct tttttagaaa acgagcgttc tgtttgtctg tatctgttgt tgatgttacc
     3301 ctgaaggtag atcaataaac aaactaagag gaggaagcac aatggcagat ttaaaaggac
     3361 gttctaaaga tatgaaagat aaagtagtcg gtgaaggaaa agaagcatac ggcaaagcaa
     3421 cagacgacaa gagcaaagaa acagaaggga aagcacaatc actaggtgct gacatcaaag
     3481 aaaaagctcg aaatttaggc gacgaagtca aagacaagtt ttctcgaaaa gacgaaaaaa
     3541 aataaaaacc acttattact aattgaatca agcaaataaa aaatccgaac catatctgcg
     3601 agacagatat ggttcggatt ttttatttga tcattatcct gttttttaaa cttcgatttt
     3661 ttgaaatttt ttatttttta gttcaaagac ttcatcacag ctttttgcta cttcaatctc
     3721 atgagtcacg atgataatac agcgattttg ttcatgggcg atttcttgga aaagtttgac
     3781 gatatcttgt gaagttgttt catctagatt tccagtaggc tcatccgcta cgattagttg
     3841 atgttcacag cagatagcac gaacgatagc gactcgctgc tgctgacctc ccgaaagttt
     3901 ggtcacctgt ttttttgcta atatttcatc aattcctaca cgagccagtc gatctaaagc
     3961 aacttgtcga taattctctt gcttggtctt agtgatttcc attgctgcta tgacgttgtc
     4021 tagagccgat aaataaggaa gcaagttgta agcttggaaa ataatcgaga catcattttt
     4081 acgataactt cttaacccga ttttgttcag tgaacgatct tgatagtata cctttccttc
     4141 ttttggtgca tctagtccag aaataagcga aagaaaagtc gtttttccag aaccacttgc
     4201 tcctaaaata gcgtacattt ttccagcttc gaaattcagt tctacatctt tataaagcgc
     4261 atcttgcgga tcttgatacc aaaatcccaa ttgttctgtc cgtaacatca aattcctcct
     4321 ttagtttaat aggatttttc ttgggtttaa tcttaatatc cctatagaag ctaaaaagac
     4381 agaaaacaga ctgattccaa atgctactcc ggctaatagt cccaattgtg caggtttgac
     4441 attgatttcc aattcgctga cttgatcaga aacagcaaat ggattattcg tggttccttg
     4501 ttgaccatcg cgttgaccgc cttgttcttg tctgttacct ggcatttgtc caccgccagg
     4561 agcttgctgg ttttgtgtcg tggtttctgt ctgttggtca agtagctgct gtcccacggc
     4621 attcgcgacg acgtttccgc taagcgaagc aactcctaat gcaagaatca tacaaatagc
     4681 cacttcggta aacaattgga gaatgatttt acttctggat tctcctaaag atagcaagac
     4741 gcctaactca tgccgacgct cccgaatggt aatcataatg atcagagtaa gtatgataat
     4801 accggctaca gccactagta aaacaacatt tttagaaaaa cttgccacgt tgtttaaagg
     4861 ttccaacatt gactgataca tggaatcatt tgattgtaag ctaaatgtat ctgtgtcgat
     4921 cagcttctca gcttctttga gaaactcatc cattttatct ggatcagata gagtgtaaat
     4981 agcagaatct atcgtgttat cgtcagatgt cccatttagt tcatttgcaa aagtgtaaga
     5041 agtgtaaagt gtattcgacg gattcatgaa gttgaatttc atacccatac tgccgcttgt
     5101 ttcgctggat tcataaattc ctttgatcgt catttcataa gtggtatctt catcttcagt
     5161 actcgtgatt gtgaaagtat ctccgactgc caggtcattt gtttctgcta gagtagaatc
     5221 aatgactaca ttgtttgtgc ctttgtcgct gtcatctatt ccttcaccat ccgtgatttt
     5281 tgctgttcca tcagaaaaat tactattttg tgcagttgct gacacaccac tgatttgaaa
     5341 gtctgcttga gtcatttgcg gattcccgcc cattcctcca gcaggttcac cgcctgcatc
     5401 agtagtttct gaactgtctt cacttgaatc agaactagtg atggcagtta ttccatcttt
     5461 tgccatagct gaggcggaac tttcaaagga ataactcttt acattatcta attcagctat
     5521 tttttccgcg tccgttaagg aaactggtgt aagagaaaaa ctgcctggat ctggacgttc
     5581 ttcagtggta gaaccgcttg aagaagtttg ttcctgtttt ttaaatgaag cttctcgatt
     5641 ggcacttaaa gtcactgttg ctccaacttc tttttgtgct tgattcgctg cttcttctgc
     5701 agcgcttcga atcgtgaggc cagctagcac aaaaataaga atggctgtga acacggcaaa
     5761 caataacaat gaacgtcccc attttacttt tgtacttttc catgcacgtt tcataaaatt
     5821 catattttgc atccctccta gtagatgcat ttgataatag acaacctatc ttaaagctag
     5881 cttaaatctt atgctgtttg cacaccatca acatgaggtt ctgtatatat aaaaagtcac
     5941 tatagttatt gcaaaaaatg tggtaaaatg aaaaaagtat gtttcaaacc gacgaagaag
     6001 aaatgaaaga ggaatagcaa aagaaatcct agatgctaca gagggatttt tacgtgcata
     6061 gttgttctaa caattgctga tactagcgat ttagtttatt tgttgtataa taatggggaa
     6121 cagctgaata cttatataag tattctggct atagcgtttc gcaaaagatc cattttgtta
     6181 atgaagtata tccaaaataa aatgcccatc caactgtcag aacgcggatg tgctggtaat
     6241 tttcctgctg attctccata agttgagaaa tagtgatttt gcatatgctt cattggaatg
     6301 gctcatatat ttagataaag aagaaaatcg acatctttaa acttggatgt cataagaaga
     6361 ggtgaagttt gtgagtacca ttaaaattat cccattaggc ggcgttcgtg aaaatggaaa
     6421 aaacatgtat gtcgcagaag tgggcgagga tatttttatt ttagattgtg ggctaaaata
     6481 tcctgaaaat gagttgttgg ggatcgacgt cgtaatccct gattttacgt atttagaaga
     6541 aaacattgat cgtgtagctg gtgtcttttt aacacatgga catgcagatg cgatcggggc
     6601 acttccttac ttgttgtcaa aaatttctgt accagtattc gggacagagt tgacgatcga
     6661 gttagcaaaa ttgaatgtca gtcgtaatga agcagccaaa ggatttaagg atttccatgt
     6721 ggtagatgag catacagaaa tagactttgg tgaaacagta gtgagctttt tccgtaccac
     6781 tcatacgata ccagattcag tggggatcaa tctgaaaaca aaagaaggta gtatcgttta
     6841 tacgggagat tttaaatttg accaaacggc tatcccaatg tatcaaacag attatgcacg
     6901 attggcggaa atcggcaaag aaggagtctt agccttacta agtgaatcgg caaatgcaga
     6961 aaatcctcag cctgtagctt ctgaagccca aatagcagat gaagtatttg atacgatcaa
     7021 atattgggaa gggcgtatca tcgtcgcttg tgtagcaagt aatttgcaac gagtgcaaca
     7081 agtattggat gcagcagaaa aagcacaacg aaaagttgtc ttgactggtc aagatttcga
     7141 acgaattatt cgcacagcga tgcgtttaga aaaacttcaa ttaccaagtg aagatctgct
     7201 tgttacgttg aaagacatga aaaactatga tccagaagaa ctgatcattt tagaaactgg
     7261 ccgaatgggt gaacccatca agtctctgca aaaaatggca aacggtacac accgtaatgt
     7321 caagattgaa gaaggagatt tggtttatat cactactacg ccaagcatcg cgatggaaac
     7381 catcgtggcg aaaacggaag atatcattta tcgagctggt ggaacagtga agttgatctc
     7441 tgagaatatg cgcgtttctg gccatgccaa tcctaatgac ttgcaattaa tgatcaattt
     7501 gatgaaacca acatattttg tgccagttca aggagagtat cgtgaattag ctgctcatgc
     7561 agatttggct catgcagtag ggatgccata caaaaatatc tatattactg gacgcggaga
     7621 cgtgctggaa tataagaaca agcgtatgag tgttgcagga actacaagtg cagaaaatgt
     7681 aatgattgat ggattaggca taggtgatat cggaaacatt gttttacgtg atcgaaaaat
     7741 cttgtcagaa gacgggattt tcgtagcggt tgtcactatc agtcggcgag aaaagaaaat
     7801 catttcaaaa cctcaaatta cttcacgggg attcgtttac gtaaaagcta gccgtgatct
     7861 gatgaaagaa agcagcaata tgattgaaac aatcgttgag aaacatttag aaagcaatga
     7921 ttttgagtgg agcaaattaa agcaagacat ccgagatcaa ttaggccgtt acttatttga
     7981 acaaacaaaa cgccgcccag tgatcttgcc agtcattatg gaagctactc aacgtcgaaa
     8041 taaaaaa
//
LOCUS       DAZABD010000096         8029 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000096 DAZABD010000000
VERSION     DAZABD010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8029
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.099"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(174..692)
                     /locus_tag="ACHALG_002582"
     CDS             complement(174..692)
                     /locus_tag="ACHALG_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFN2587911.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(694..1311)
                     /locus_tag="ACHALG_002583"
     CDS             complement(694..1311)
                     /locus_tag="ACHALG_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFN2587912.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(1308..2162)
                     /locus_tag="ACHALG_002584"
     CDS             complement(1308..2162)
                     /locus_tag="ACHALG_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN2587913.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(2159..3070)
                     /locus_tag="ACHALG_002585"
     CDS             complement(2159..3070)
                     /locus_tag="ACHALG_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN2587914.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(3111..4445)
                     /locus_tag="ACHALG_002586"
     CDS             complement(3111..4445)
                     /locus_tag="ACHALG_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN2587915.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            4653..5483
                     /locus_tag="ACHALG_002587"
     CDS             4653..5483
                     /locus_tag="ACHALG_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HFN2587916.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(5727..5976)
                     /locus_tag="ACHALG_002588"
                     /pseudo
     CDS             complement(5727..5976)
                     /locus_tag="ACHALG_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206386.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6167..6361)
                     /locus_tag="ACHALG_002589"
     CDS             complement(6167..6361)
                     /locus_tag="ACHALG_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587917.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(6866..7051)
                     /locus_tag="ACHALG_002590"
     CDS             complement(6866..7051)
                     /locus_tag="ACHALG_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587918.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(7901..>8029)
                     /locus_tag="ACHALG_002591"
     CDS             complement(7901..>8029)
                     /locus_tag="ACHALG_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFN2587919.1"
                     /translation="IKGQVIITQLKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF"
BASE COUNT         2642 a         1546 c         1346 g         2495 t
ORIGIN      
        1 caccaattat acaacattta ttattaaaat taaattataa aattttataa cgttctcatt
       61 taacaaaact taagttatta agacaaatga ataaacaact acgctttcct cgaaacaaaa
      121 aaataaagca caaatgcgca tttacgcact catgctttat tcgtacaatt aattcagata
      181 acggcagcgc ataggatgcc aataacactt cctaaaatag ctcctcctaa aatgtcagta
      241 ggataatgta aaagtaaaaa aatcctcgaa taagcgatca aacaagcaaa taaaaaggct
      301 aatattcctg caactggact taattcgaac aacgcagcta ctgataaaaa ggctagcgct
      361 gtatgtcctg aaggaaaaga agaagtcttc ggtttacttg tgtaaaggac aagccgacga
      421 tttttttcat aaggtctttt ccgtcttgtc gtttttttga taaaaagcaa accaacaaaa
      481 aaatagatca aactgccgat aagaatggct agaccatatt ctctagtatc aggtatcaag
      541 actaaaacag cagcaaggag caaccaaata aaaccaccat tagagatatt actgataaat
      601 ttcaaaaaat tgatttttcg actatccgtt ttccgttgct tggcgaactt gttgataata
      661 ctatcatcga ttttagcaat caaacgcaac atgttattcc tcctttttaa gaaccttttc
      721 ttgcgtacga tggataaaaa gcaggacttg aaaataaaac aagaaactga aacctaataa
      781 agcaaaatag attggtcctg caaaccagaa tgcgacaaga aggatcaacg tgcctgccaa
      841 atagaaaagc gcgcttttga aatcattaaa aaacaagaaa aatgcagtaa tccaagaagc
      901 ttctttttgt tccagttgaa gcaagatttt gtaactaaag agaagcgcgc ttaatagaag
      961 caaaaacaac gtagtataca tcaatgtata gccaattccc ccaccccaat agcggaaaat
     1021 ctgataatcg atcaatagaa gaagaaaata aacacttgct aaactatctt gctttccata
     1081 aagagagaag ttctcacgaa actttttcca gaatagtgaa ccgagctttt tctcttcatt
     1141 ttttttgtaa tgataaatca tatacagaac tgttcgactt gcagttccta atgtgaaaac
     1201 aggcaagacg cacaggaacc atagtagctg taaatggata agacgtggaa tccattcaat
     1261 gatttctaag aaataatcat tggctttttt accaatcgat cgattcatca tccttttgaa
     1321 cctcctgtca aatccatccc ttcaatgaaa taacgttgtg caaaaaagaa cagcaaaatc
     1381 gtcgggatca agacaagtgt tgctcctgcc atcagataat tccattgtgt cgttgcttgg
     1441 ttctgaaaaa cttgcaagcc aatctgtaac gtgtattttt cttgatcttg gatatataac
     1501 agaggaccta agaaatcatt ccaagctgcg ttgaaggaat tgatcgcaat ggtaatcaag
     1561 gcaggtttcg tcaatggaag catcaaatga ctccaaatat acaaatgatt cgctccgtcg
     1621 atttcagcag cttcgatcaa ttcattattg atcgataagt aaaattgacg catcagaaat
     1681 atattgaacg cattgccgaa gaacgatgga acgatcaggg gcaaataagt acctacccag
     1741 ccgatcttgg agaataatac atattgaggg atcatcgtta caaaccccgg gatcatcatc
     1801 gtagaaagaa ccaaagcaaa catcagcttt ttcccaggaa aatcaagctt cgcaaatcca
     1861 taagcaataa aagaatttga aaggacatta ccgatcacta ctagcacagt gatgaataat
     1921 gtatttcttg catagcgcca aaaaggaaag gcggcaatcg tatccagata gttttcccat
     1981 acgatttttt ctgggaaaaa ggtcggagga taacgaacga tttcttccat tggtttcaat
     2041 gaagtaaaca ccatccaaag caatggaatg atcaaaatga ttcctattac tgtcatgagg
     2101 acataactta gtattttttt tgtttgccga ttcacactgg tctttttgaa ataatccatc
     2161 atttgccgtc acctccagaa taatagaccc acttaggtgc aagcttcaaa ttgacaaacg
     2221 tcagaaccaa cacgatcaga aacaggatca ttgacatagc catcgcataa cccatatcaa
     2281 aagctacgaa cgccttgttc cacatatgca gattataaaa aagcaaagaa tttgcaggtc
     2341 cgcctgttcc atttttcgtc atgacataag cttcttgaaa aatttggaaa gaaccgatcg
     2401 tagaggtgat cacatcgtaa aaaataatag gagtgattaa tggcaaagta atacttttga
     2461 actttcccca aacgcctgct ccatcgattt ctgctgattc atataaagat ctaggaacat
     2521 tttgtaatgt agctaagtaa agtagcattg ctcctccaga actccatatt ttcatcaaaa
     2581 tcaacgcagg tttcgtccat tgtggatcaa acagccaagc tggtccaggg atatggaacc
     2641 agcccagaaa tgtgttgatc aatcctgcag atggagaaag cagctgcatc cataagatgt
     2701 agactgctac tcctgataag actgccggta aatagaaagc tgtacggaat acactgattc
     2761 ctttgatttt ttgattgagt actgtggcta aaaacacact gaaaatcgtc gtcagcggta
     2821 cattgaagat gacgaaataa acggtgttgt ataacgattt ccaaaataaa tcatcgtgaa
     2881 cgaacattct tttgaaattt gctagaccaa tgaaattcat cttggatgtc aaattataat
     2941 cggtgaaact accaataact gaaaaaagca taggccctag cataaatacc aagaacccga
     3001 taataaaagg cgagataaat aaataggccc atcttgcttc ttttttcttt cggatagata
     3061 aacgcttcaa attttcttgc ctcctctgta cctagacagt caaaaatgga ttattgattt
     3121 tgcttgacta agttttctac tgctttttgt gcatctgcca atccttcttt tggtgatttt
     3181 gttcctaaaa gaatctggtc aatctgttca ttcactaatg tcgtgtaatc cggagcatct
     3241 aaaggaactg gtgtgatcac cgtattttta aagttttcgt ccgccatttg atagatcatt
     3301 tttcctttat ccgttaggtt gtcattattc actaaattct cattggctgt cttgctcgcc
     3361 atgatatcaa aacttttttc gccgaatttt tcttgtactt ctggagaaga aagatatttg
     3421 atgaattcat aagaggcctc aggatctttt gctccgtatg gtacttccaa gacgaatccg
     3481 ccaccccacg accaatggcc tgaaccttct tctttttctg ggagctgcat cacgccaaaa
     3541 tcaaaatcat ccggtgcatt ttccatcaaa ctagagtaat aattgatatt ttgggcacgc
     3601 atggctacta atccggaaat aaatggatca gatacaccag acccaaattc ggcttctaat
     3661 ttattgatcg tatttttccc ataataatct tgccagtctt ggatccactc caaggcttcc
     3721 actttatttt tagtatcaat cttgactttt tcatctttgt caaaccaact agtcccgtta
     3781 tctgcgttta gcgcccatac atcagcccct aaattccata atggataaaa accgatgcgt
     3841 gtaaattctt ttccttcttt tttatctagt ttacgtgctg tttcttctaa ttcatcccaa
     3901 gttgtcggta gatcttgttc actgattcca gcttctttaa ataacttttt attataaaaa
     3961 atgacttgtg tatctgtatt gaatgggatc gcataaggat caccttcata ttctactgta
     4021 tcccaaagct gcggataaaa atccttagaa gtgtcttttt ctacatattt ggagagatta
     4081 gttgcttgct gtgcttctgc tctttgggca acggaattta tgtcctgtac aataacatcg
     4141 ggtgcatttc cagctgttat tgcagataaa gatttagtcc agatgtcacc ccatggctga
     4201 tacttataat ttacatggat ctcgttttgt gattgattga aatcctcaat gatttcttcg
     4261 atagcttctt ttcgtgcacc ggatccccaa aaggaccaga aatcaatatt ttttcttcca
     4321 tcacttgttt tttcagaaga atttgctgca cttgaactgg catcgcttgc attacttgat
     4381 ccgcatcctc ctaataaaaa caatgctgaa cttgccagca accctgtaaa caataatttt
     4441 ttcatatttt acctccaaat tttaatgtaa ccgatgccgg ttttaatata acgatatcat
     4501 tttgttctga aaataacaat ttataaggta tgattctaaa aaaattatta atgataccaa
     4561 tactttatat aattcgcgag cgctttcttt gaatttttta atctttcgta gcagaatgag
     4621 aatgtattaa aaatagatta ggaggaattt tgatgtttag acaccagaaa gaattgcagt
     4681 ttgaagcaaa accagatcgt ccgaatccat taatcgcgaa atatctgcag gagctgatcg
     4741 gcggccaata tggcgagatg agtgtagcta tgcagtacct gttccaaggc tggagctgcc
     4801 gaggagaaga aaaatataaa gatatgctga tggacattgc aacagaagaa atcggccacg
     4861 tggaaatgtt ggcgacaatg gttgctcgtt tattagaggg tgcgcctgtt gaagatcaag
     4921 aagcagcgat gcaaaaagat ccaacaatcg gtgccattat ctctggaatg aatccacagc
     4981 acgcgatcgt agctggctta ggacctcgtc ctgctgacag cgtgggcaat ccatggagcg
     5041 gtgcctatat cattgcgagt ggcaacttgt tagcagactt tagagctaat ttaaatgccg
     5101 aatcacaagg aagacttcaa gtagcacgta tttacgaaat gatcgatgat cgtggcgtaa
     5161 aagatatgct gtctgttctc cttgcaagag atagctatca tcaaaatcta tgggaatgtg
     5221 cagtgaaaga attagaagaa aaagaaggtt caatcttagt cccaagtact ttcccgcgtg
     5281 acaaagaacg aaaagacatt gcttatgact tctataactt ctcagaggga gaccaaagta
     5341 gtgaaggttc ttgggctagt ggcaaagcac tggatggcga aggcgaatat caatggttga
     5401 aagaacctaa gatcgaaggc gctgcaccaa aattagaacc agtaacacca aaaatgtatg
     5461 ggacacctcc attcaagaaa taaatatcta cccgtagaaa cacccgtttt ttgttccaca
     5521 cgctttgtga gcaaaactga ctaaagtcat taatcataac cacccttcaa aagggtggtt
     5581 ttctctagag ctataagctc aaatcaccgg ccagcgccta aagacgctgg ctttcatatt
     5641 ttatgttcaa gccatattgc ctttgccagt tttgcccacc tcttaaaagg tttatgtact
     5701 actccttagc ccttaaaagg atcgtcttac tctcgtacac tcagcttatc taacgcacta
     5761 tcatgttttt cttgttcttg aatgtacttt ttgatggtcg cttcattttg tcctactttt
     5821 gtcacatagt atccttccac ccaaaagtgt cgatacccaa atttatattt tagatttgca
     5881 caatctcttg atgatctcct gaatattggc tcgatattga ttataaatta tttttcttct
     5941 atactttggt gtgaatacaa tgtggtattt acacatctac tttgtacggg ctaaactatt
     6001 tccctttatt gctccacccg catagcaagt ggttttgtat ttcaatcgct cggcgcgctc
     6061 aattaggtct aaagacaata aatctaaaaa cccgctgctt cactgacaga aacaacgggt
     6121 tttcagaaaa aaatgaaatg aaaaagatct cttacggcaa tctattttat ttttttgatt
     6181 ttttataaat ggaaaatgct tcaagaagtc caagaaataa agccgtataa aaacaagaac
     6241 ctatgaaatc tttatttatt agatattcaa cagaaatccc gataacacta gccaacaata
     6301 tatttatcag aagtgtaaaa taaggatgct ttttatagaa tagaactagt gttgttttca
     6361 tagactctcc tttcttcata tttccaatta atcacaaatt cacggcatta ctagaaaaat
     6421 taaaggggcg cagaagtgga aaaatactct tcgactttcc ctacaaacca ggccatcttg
     6481 tgtttaataa ctataccaaa tctaaggtac ctctaaatct aaattccatt tgtggcggtg
     6541 acctccaatt tttaactctt ctatctccca tccagtatac ataaccttga tacttcgacc
     6601 acttatatat cgaacacaca aatacctatc cattgcagga gtactaccta acttagttgc
     6661 tacttgcatc taaataacag ctgaaaagat aggacttact cttagtaaag tatctgaact
     6721 attaattttt aaactatcat ttttatcctc aactacatgc acatctgttg caacttcact
     6781 ttctgtctca tccagataaa tgattaagga atcgggaatt gcaatatctt cagctgacgc
     6841 tggaatagtt gagctaaatt agtcatcata tctttattat aaaataacca acagcaaaaa
     6901 atacaatgaa aaacaaactt ggaactaatg tatcattaat aaatgttgaa aaattcagca
     6961 actcattact gtatgatttt agcagcccaa ccaacatata ccctataaaa gctaataaga
     7021 tggttaggaa tcctttagaa tttttactca ttcatagaca cttccttaaa taacttttat
     7081 tatagaaggt attgtaaata ataatattga caaaaagtga ctcaaaataa aaaaaaactt
     7141 acattatact ggaatgaact gtattgatag tgtacctaaa agctctactg acataagatc
     7201 gtgtaatcgg taaagtaatt ttattaatta tacgggatgt aatcaattag agaataaact
     7261 aatcataaaa ggtactttta cattcataat tgtcgctttg agattctact ttggttcact
     7321 atctgaccca attgcacatt ttcgatcatt acaagtattg gtgtcgttag aacaactaaa
     7381 taaaaactag cggtagattt ttcagaatct atgctaattt ctgtatggat gttccagtga
     7441 tggcaaattt ctcttttcat tgtttttctt gtcgcatgat atgccttttt gtgttattca
     7501 gttaaactat agaaattctt tcctagacag atcttgatcc caaattttac gtttttgagc
     7561 atcgatatag tttaaaatga cattactcat atctaaagag tttagatttt ttaggactaa
     7621 aactgtcagt tgttcttttt cttctttctg ttaaagtatt gtcgcgactt ttcgtatacg
     7681 ttaagatatg tcgtgaaaaa acaagttttg tgacacaaaa aaacgaatga cttgaggaaa
     7741 tcacctcaat cattcgttta aaatgctata tattctttag tttaagatac ggaaattaat
     7801 gccatcgacc acactagtag aatgaacgac atttacttgg ccttttttta atttcgttat
     7861 gtttagcata taaaaatatt atagagttta cttacaatgc ttaaaacata tactgtataa
     7921 tctgatcaat tttagccatg tcttgcaatg gtaagttttc tatttttttg agttttcgtt
     7981 ccttaaaatc aagagatttt aattgtgtaa taattacttg tccttttat
//
LOCUS       DAZABD010000097         7863 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000097 DAZABD010000000
VERSION     DAZABD010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.100"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(188..475)
                     /locus_tag="ACHALG_002592"
     CDS             complement(188..475)
                     /locus_tag="ACHALG_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFN2587920.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(495..719)
                     /locus_tag="ACHALG_002593"
     CDS             complement(495..719)
                     /locus_tag="ACHALG_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFN2587921.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(1174..1344)
                     /locus_tag="ACHALG_002594"
     CDS             complement(1174..1344)
                     /locus_tag="ACHALG_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587922.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(1359..1892)
                     /locus_tag="ACHALG_002595"
     CDS             complement(1359..1892)
                     /locus_tag="ACHALG_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFN2587923.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(1913..2857)
                     /locus_tag="ACHALG_002596"
     CDS             complement(1913..2857)
                     /locus_tag="ACHALG_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFN2587924.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     FTFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKIN
                     GDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFA
                     YFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELL
                     QQIKQLEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
     gene            3494..3580
                     /locus_tag="ACHALG_002597"
     CDS             3494..3580
                     /locus_tag="ACHALG_002597"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFN2587925.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(3701..4390)
                     /locus_tag="ACHALG_002598"
     CDS             complement(3701..4390)
                     /locus_tag="ACHALG_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587926.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(4397..4675)
                     /locus_tag="ACHALG_002599"
     CDS             complement(4397..4675)
                     /locus_tag="ACHALG_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587927.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(4635..5549)
                     /locus_tag="ACHALG_002600"
     CDS             complement(4635..5549)
                     /locus_tag="ACHALG_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFN2587928.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(5531..5911)
                     /locus_tag="ACHALG_002601"
     CDS             complement(5531..5911)
                     /locus_tag="ACHALG_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFN2587929.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(6219..6374)
                     /locus_tag="ACHALG_002602"
     CDS             complement(6219..6374)
                     /locus_tag="ACHALG_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587930.1"
                     /translation="MKLTKLTISLVIIAGIILYFVLPANQFFLYFGIPTILIVNGVRI
                     FEQRKKM"
     gene            complement(6393..6962)
                     /locus_tag="ACHALG_002603"
     CDS             complement(6393..6962)
                     /locus_tag="ACHALG_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFN2587931.1"
                     /translation="MTIKWIISIAYLVLTFAIPSLGVPSNIYFLFEHSDIFFLVLIII
                     LFHSTFIREFKEVNLKKLFKYSFFSFSVLFLINILNTSFSEGINPNEVNGSLLLFFLN
                     AATYGAFLEEGIFRFCMIDPQANKKQQYISILISSFLFSIVHGGGLSIFFIGIILSFV
                     YIQTKNIWYSIVAHGFYNTIGILIYLISI"
     gene            complement(6962..7417)
                     /locus_tag="ACHALG_002604"
     CDS             complement(6962..7417)
                     /locus_tag="ACHALG_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LMxysn_1693 family intestinal colonization
                     protein"
                     /protein_id="HFN2587932.1"
                     /translation="MKKQLFKTFLLSTSILTFGSAITPSGAVFANENNISDIATYSDD
                     EYYYVLDEPVIQTRTWSKPQSWSIAKGSRTYLGIETHSRGTKLSVSANVFGMKIEIAR
                     NTSASGKFKKYRQNARITVTYRVYRKVDNKYIRTQTSTANTQYIDYIPA"
BASE COUNT         2214 a         1392 c         1304 g         2953 t
ORIGIN      
        1 ttcaccttcg gtgaaattgt tgcaaatcaa taattaggta gagctacatt tttttcgttg
       61 ttcaaagatt cttatgccat ttactattaa taaatctata aatatgataa tttttcggct
      121 ctataattta tgggactgat gaatcagaat tgattcttca gtcccatttt cattttattc
      181 caaagaatta actccaaata ccaatagaag cccatcctct atatacttga gattccttac
      241 taacaatttc ttgtaattct attaaagatt taggtaatcc aaatttatgt tttaaaatat
      301 atcttccaag aaaactactt aatttcatac taacttcacc aacacttttt ccctcactga
      361 tttctttaga ataaagaaag agcttttctt ttaagtcagg atagtcatct atagttgctt
      421 cattgtatac tttactaatt gcatttaaaa gtttttcttc tttagtaaaa tccattataa
      481 taacctccct accactacct tctaggtgcc catggaccat gattaaccca accattaaca
      541 ataatttttc caatttctcc tttagcttga ttccaatcta cccaacattt ttctttgtta
      601 caataaagac catttccata gtaagtagct gcatcaactt ttattcctgt accgatgcca
      661 gctagacaag ttcctaatat tcctagaata acacaatgtt ttaatacttt ctttttcatg
      721 attgtttttc cttttcgttt ttttaaaatt gcttgttata ttaaataatc ttagtaattt
      781 tattatataa cattttatta tataacaatg attaacgtca taaaaagtag ttttgaattc
      841 ataatcgttt aaataaaatt ataagatcag ttatttaaac agctagtaga cataatgtcg
      901 atactaccaa gtaacgcctg taccaaggga tccaggcgct gattttgtga atttttattg
      961 gtctaggtta aaagctcttt ttaaggcttg gttttctgcg gttgtttctt tctaattctt
     1021 tgtgaatatt gatcggtcat tttttaaata cttgttattt ttgtttgtgt ttgttatgct
     1081 ttttacatat ctcatattca tttattattc ttttctaagg ttaataatag gataatctct
     1141 ttcagaaaga ctgctcgtac gagcagtctt ttcttattta tcaagttttt ctacaaatac
     1201 ttcctcattt tgactgttgt agtaattagg attgagtttt agttcccctt ttttatttcc
     1261 tttgttggcc actgataata acattgggct aaagcttaat attcctagta ggatagaacc
     1321 gaatagcaag tgtttttttg tcataagtat ttccttcttc atttccatag ctgccaccat
     1381 tttttccaat tctttgtttg ttcgtccatt gtttctgttg cttcagaaag agtcgttgaa
     1441 ctattctctg aagactcttc cgtataagtc aactgcagtt gctcctgaag tttttcgatt
     1501 tgttggttgg cttgcagggt gagttgttgt tgctggtcga gtagtttttg tatatgagaa
     1561 ttattttgct ctttgagggc aagttgttct cgtaaaaatt cattttcttc acgcaactga
     1621 tctagcaact tgtttgcaac cacgtttttg tttggtatat aagcattttt atcgttgttt
     1681 tgttcatgtt tatgcaactt atctagcaac ctttgattgt ctaagttgcg agcaagttgc
     1741 aatactccag aacgcaatac aaccttataa ccacgctcat ttttagctat catggaagaa
     1801 tctaattttt ggatatgttt cgatattgct tgcctactca tcttaaaaag atctgacaat
     1861 tcctttattg taaaggtttc ttgtggttgc atactggttg cctcctaggt tgctactgga
     1921 ataaattatc taaatcagat aataaacttt ttttctcttt ttccgaaagt gcggactctg
     1981 tttgtttttc aagagctttt ttctcttttt ctagttgttt gatttgttgg aggagttcaa
     2041 gttctcgttt cttctgtttg attttttcgt taattgttgt agcgggttct tcgtattctt
     2101 tttgtttatc agctaaagcc aatgctttat tttggtcttc tttggttggt gcatgtttgt
     2161 taaagtcgtc tgcttttttg atctcaggtt tgaacgtaaa ttgatagcca ataacaggtt
     2221 ttccccgtcc ttttccgtat ttctttttga ttgttagtcc tttgaagatt gcagttagtt
     2281 cttgacgtat aggttttata actcgtttat ctatatcagg ttgcttataa cttttaggaa
     2341 tatctaataa ttcaagaaag tcttctttgg agaaatatgc gaaacctttg gttctgtatt
     2401 gctttaacag acgaaacata gtctttgcat agccactttg tagctcgttg aattgttgta
     2461 aagaatagcg tacccactcg tctaaattat ttaacaaagg cagtgctttt tcgtaaattc
     2521 gtatatctac gtaaggattt tctacgtccc cgttgatttt aaattctgta aacattacaa
     2581 acatatcgta agaaagccca gactcacttc tacgaccaaa gcgtaaagat aagatttttt
     2641 cataagttgc ttttaaatct tctacaaatc gtacatttgc agttgctttg tagttactta
     2701 aatcttttag ttggtcaaac gtaaaacgaa ctgtcttatc ccctttttca cgcattctag
     2761 acacgatgga aaaaaacaaa ttcatttcaa ccggtgtaaa ttttcttaaa ggaattgtat
     2821 ttaattcatg gtggtatttt acaatctcat tagccatata atcacctcaa tccaattatg
     2881 aatgacattt tttaaatgtc aagacattca tatttgacgt tcaaacagac aaaatgtcct
     2941 tcataaacag acaaaatgtc cttcataaac agacaaaatg tccttcataa acagacaaaa
     3001 tgtccttcat aacattctgt atccattggt agagtaagga cacagcttac ggtaaagagg
     3061 tttaaaaaga ggtttaaaaa gagatataaa aagagggggg ctaattttcc cgcctaaaaa
     3121 ccacttcttt tttctttaaa caggttcgcg catttttcaa aactgccaat ccaaaacctt
     3181 gcttcctacg gtcgccaaaa aggtgtgatc gctacgctct cacattgtac tgcgcagtcg
     3241 cctaaaggct cctttgctaa aaacatgttg gtaaagaggc ctttgagcgg ttctgaatgc
     3301 ttatctaagc cgttttaaga cagtcttgga taaaaatcct tatctagctt taaagcgcct
     3361 tacaggcggt cagggcaagc cctaacaacc ctaaaataag cgatacttaa aaacaccaca
     3421 caagtttaat tttttaagat ataataactt gtcgcctatc cgacagtctt gcgactggaa
     3481 ggaggtgtat ctaatgtttg aattgttcat ttgccccctt cttatagaag tttgtgtctc
     3541 tttattcagt tattggttga ataaccgaaa caagaagtaa aatcaataaa ggcgacatca
     3601 acctgaaaaa aagccccaga gaatccgacc tctctggggc tttcgtgtat ctaatgtttg
     3661 atcatttgca tgtttagttt aacatgattg ccgatcaaat tcaattttgc tgttgctgat
     3721 ctaaccattc cttgacactt tttagataat ttaatgccgt ttcttttttc gttggatttt
     3781 gtatgttcgt tgcttgctga agcagtgctt tataacgttc gtaggtaact gattgttgcc
     3841 gttcttttcg ttcctgttca ctagttgaaa aagggattgt atcactgaat aaattataac
     3901 aagcttcttc tgtctgcgta tctttaaaat attgcacgtc ttttttggtt cgctcatagt
     3961 tgtcttgaac ctctttttgc tgtttttctg gcaagttttg ataaagacga gtgagttttt
     4021 ccatgttttt atccgtcaaa cgtttttctt gattcacaaa aatatcgttc atttcctcat
     4081 aacaagtggt aaactcttcc attttttgtg ttaacacttg ctcctttttc tttttatcac
     4141 atgctgcttc atacttttga gtgatatccc ctaataaaat acttcttgaa atacgtttat
     4201 ttccctctaa aacgccgaat aaaccactta aaacgattga taatgtaaat ataacccctg
     4261 gcaataaaaa cctcttaaat cgaattttag agccttttaa tgaatatctt gtttgataag
     4321 ccttatagct aagccaaata attcctaaaa tactggcaaa aatgagtaga tagggtaaat
     4381 agaatgccat ttctatttat tctccccata ccagccaaaa ggctaacagt gtcatgatta
     4441 agcaacataa gggggcaaga atatccattg atcgctgcat aattactttt ttctgttctt
     4501 tatttcgctg gtttaatagc gcatggtgga attttttgac ttgtgaatca attgtagttt
     4561 gtattctttc tttggtataa ggtgcaagaa aagcacgttg tttctttatt tcattttcta
     4621 tgtcttgtaa cgttttagaa cgataattct tgttggtagt ctgctcctct ttcaacgtgt
     4681 tggtcatggt ttcttcgagt cggtctaatt tccggttgtt cagtcgtaag agttcgttgt
     4741 tctcttcgta cttcttttgc agtttcgcga atgctagctc gagctcttcg tagctcgttg
     4801 gttcgttgtt gattgtaggc tcgttctcgt ctgtccagct catgtaaaat cgtctccttt
     4861 tcgtaatcct ttcctagttt gttggatcgt acttttttct ttttgtggat caattcgtaa
     4921 gtaaaatttt tcccacgatt caagcaatga atgccgaatg ttttaagaga atcacaaaat
     4981 tggttatacg ttcgagtttt tttgtttttc attgtttgat caatcgcaaa ccgaatttcg
     5041 tctttccaaa caggttcatt tttttctttt aatttgattt cagacatggt tcgtttttcg
     5101 ggtttcttcc ctcgttcagt gattgttaag tcatgttcaa gacaaatctc gtcatttagg
     5161 tcataacatt tgtttaaaaa tgcttgacca tgggcttgaa atttgttccc agtttccaga
     5221 ttgacggagt taatgacaat gtggttatga atatggtctt tgtctgtatg tgtgtaaatc
     5281 gcgatttggt ggtcaggggc tatttttttc gcatactctg cccctagttg attggctttt
     5341 tcagcggtaa tttctcctgg tcgaaaagac tggatcaata agtgtgcttg tacacgatcg
     5401 tcttttccat acagcatgcg aatttgtttc atttcgcttt tggcataatc aatgtcacag
     5461 tttatccctg ttttgacggt tgctcgtggt tcggcgtaat tgatcgctcg gctacaagaa
     5521 gtggttcggc ttactttaac tgttgccata tcttttgtag ctccttttgc atgattttta
     5581 ggttttcgga taagcgttgg gcgtcgccag gatcaacgtc gtgttggtgt gtgttgcacc
     5641 acttggcaat ttgattgata ttcccgccag ctttggctaa atcgcgcgcg attgcctgag
     5701 caatgtctgg cgccactttc ggtgtcacca gacgcgcgtt ttgtgccttg gctttcacga
     5761 aagccggcac actcatttgc aaagcttcag ctgactgctt cagctttgca aattcggatt
     5821 cgctcactcg aaaactgatt tgttttggct cggatcgata ggtatatttt ttagaatgat
     5881 tgctagccaa atattgattt tgttcgctca taagtcccac cctttcagta cgtcctacgg
     5941 acgtatcgtt cgcaatacca ttgtacctaa aaaggtacaa taagtaaagc tcactaaagc
     6001 tcactaaagg gcttggcaag ctctatattt gctagccaaa tacctacgta tttggtgcaa
     6061 ataaccgctt gtcaaagggg cgcaagctcc cccttgaatc ccccctcaaa acccacgctc
     6121 cacaatacaa aaaaggctag caaatgctag ccactttttg taaagtttcg cttgtttcac
     6181 cttcggtgaa attgttgctt tgcaacgatc aggtagagct acattttttt tcgttgttca
     6241 aatattctta cgccatttac tattaaaatc gtaggtattc caaagtaaag aaaaaattga
     6301 ttagcaggta atacaaaata taaaataata ccagcaataa ttaccaaact tatagtaagt
     6361 tttgttaatt tcatgaagta tgccacctta tcttatatag atatcaaata gattaatatg
     6421 ccgattgtat tataaaatcc atgagcaact atggaatacc atatattttt tgtttgtata
     6481 taaacaaaac ttaaaataat accaataaag aaaatactta ggcctccccc atgaacaata
     6541 gaaaacagaa aagaagaaat tagtattgaa atatattgtt gttttttgtt tgcttgagga
     6601 tcaatcatac aaaatcgaaa aattccttct tctaaaaaag ctccataagt agcagcattt
     6661 aaaaagaata aaaggagaga accatttact tcattaggat ttataccttc tgaaaaagat
     6721 gtatttagaa tattgattag gaataaaaca gagaatgaaa aaaaactata tttaaagagt
     6781 ttttttagat ttacttcttt gaattcacga atgaaggtag aatgaaatag tataattatt
     6841 agcactaaaa aaaatatatc actatgttca aataaaaaat atatatttga cggtactcct
     6901 aatgatggga tagcaaaggt caaaaccaag taagcaatac taattatcca ttttattgtc
     6961 attaggcagg aatataatca atatattgtg tatttgctgt agaagtttga gtacgaatat
     7021 atttattatc taccttacga taaactctgt aggttacagt tattcttgca ttttgacgat
     7081 attttttaaa ttttccactt gcagaagtgt ttctggctat ttcgattttc atcccaaata
     7141 catttgcact gacagataac tttgtacctc gactatgagt ttctatacct aaataagtac
     7201 ggcttccttt agcaattgac cagctttgag gtttagacca agtccttgtt tgaataactg
     7261 gttcgtctaa cacatagtag tactcatcat cagaataagt ggctatatca gaaatgttgt
     7321 tctcattagc gaataccgct ccgctaggag taatagcaga accaaaagtt aaaatagaag
     7381 tagataataa aaaggtttta aataactgtt ttttcatcaa aatcacctcg aatataagat
     7441 acttaagaaa acgctttctt tatataatta gaataatcag gaaatttaat gttgtcaaga
     7501 gggtttattt tataaaagct gttatattaa aggttagtag acataatgtc gactatgcca
     7561 agtagcccct ctaccaatgg atagagacgc tcactttgtg atttttaatt ggtctagcta
     7621 gttactagct tctaatgctt gattttacgc gatttctctt ttttagttct ttgtgcattt
     7681 ttatctgtca ttttttaaag acttgttatt tttgagagga tttgatatgc tttttttgtc
     7741 tttctctagt tcttcacttt gtaatatttt tatgttgatg aataggataa tctcattttg
     7801 ggctgctcgt gagagcagtc tttttttgtt tcagggtacg tcctattctt tggatttccg
     7861 cgc
//
LOCUS       DAZABD010000098         7757 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000098 DAZABD010000000
VERSION     DAZABD010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7757
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.101"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(14..712)
                     /locus_tag="ACHALG_002605"
                     /pseudo
     CDS             complement(14..712)
                     /locus_tag="ACHALG_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
     gene            791..967
                     /locus_tag="ACHALG_002606"
                     /pseudo
     CDS             791..967
                     /locus_tag="ACHALG_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(1143..1712)
                     /locus_tag="ACHALG_002607"
     CDS             complement(1143..1712)
                     /locus_tag="ACHALG_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFN2587933.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1786..2109)
                     /locus_tag="ACHALG_002608"
     CDS             complement(1786..2109)
                     /locus_tag="ACHALG_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587934.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2263..3075)
                     /locus_tag="ACHALG_002609"
     CDS             complement(2263..3075)
                     /locus_tag="ACHALG_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587935.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSELYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3402..4277
                     /locus_tag="ACHALG_002610"
     CDS             3402..4277
                     /locus_tag="ACHALG_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFN2587936.1"
                     /translation="MGKRSTIQLMIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGT
                     FSRVQCTPDLLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLE
                     AMAENHATIDNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDD
                     ELLLLLDKFEKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAI
                     QLGISPRGSLSFIQAAKAYALIHGRNYVTPKDLQELVPFVFSHRLIFYDRSLKEDEKT
                     QMIHALVAQIPIPVR"
     gene            4281..5354
                     /locus_tag="ACHALG_002611"
     CDS             4281..5354
                     /locus_tag="ACHALG_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HFN2587937.1"
                     /translation="MEKAKDYFINFVLFLIYFVIVFFALVFNNSLGWFLLAFFTLYVS
                     WSVFSLSTFLKKLSVTCTVQPVVYRKKSIQVSFSIAKKRAFSFPLPRLQIVFPEIFAE
                     KKQEILILTNKPQEVQVLWQPKERGFHEALTVIFTAYDSLGLFRKRTRRELSFPVTVL
                     PAFHKSQAEALQRVLEKKQQLNAYQKGLDFREHRSYRPGDSFNRIDWKLSAHSQEWLY
                     REYEYQEEEYSPLLCFWGSPSLKFDHLLDLYFSLWHLRKEKQPELLILGDRTFHGKDP
                     GYTYFASLNAGDEKTFLAHLKKQAKKQIVLFIPAHSPEVSEAVETLSKDRTVYLVYFA
                     EKQIMIQEKDKVCSLPGGEWIDD"
     gene            5344..7514
                     /locus_tag="ACHALG_002612"
                     /pseudo
     CDS             5344..7514
                     /locus_tag="ACHALG_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342468.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing transglutaminase
                     family protein"
BASE COUNT         2298 a         1558 c         1418 g         2483 t
ORIGIN      
        1 agggggggat tttttatcgt tgtggaatat ttaaatacca acgcttgtca tgaaaatctt
       61 gtgctccacc cttagtattt ccttctggat cgtttgtcgc acgcatcatg acgtatactt
      121 tcttattagg gaaatcacgc atattgaaag atacatgata gccaacattt cctaaagtat
      181 tataagcttg atttacgtct ggtctataaa tcccatcagc tcttactcta gctagttctt
      241 ttccagtatt gtagtccata atgaaaatat actcgtattt ataattagca atgtgccatc
      301 cagctacatg caagtttgcg ttttcgattt ctccgaactg atcaatgtgg gcgtaatttg
      361 ttccatctgt cagcgtagaa tttgcagcac ctgctcgagt tggatcaatt actggtttat
      421 cctccgaagt agttggatta tcatccgtaa aaccatgagc caaatcatag gctagttttt
      481 ctttacttac acctatttca gaaagataac cgtaaggatc tgtatgatcg ccccaaatat
      541 tttgtgttac ccataaatgc gatttgattc ctggttggtt ataaggagtg tctaatgtta
      601 atggaatacc atatttcatt gctgaatctc ttgccaattc aacgtatact ttatagttct
      661 tttcaaaagt ttctttatca tgtgtgtgtt gtaactcgat ttgcacagga cttttggctc
      721 acgaggttgt cctactctac atcttgctga tagatatgct gaaaggaaaa caccaacatt
      781 atgttttgct ttttcagcta gaacgagaca agcaggaata cctttagaag atatcaaagg
      841 ttttttaggt cataaagatg tttccactac acaagtctat gcccatattt ctcccgaagt
      901 gaaaaaaaga tcaatgaatc aactagaaaa ctatatagaa gagcaaataa aaaagcactc
      961 aaattgagtg cttttttaag ctcactatca ctaaaactat cactaatttt atttataagc
     1021 gaacatgttt ctatcactga ttttttaaat ctagcgaaaa acccttatct taaaggcgtt
     1081 cgttcaattc tttggcaagg tcttcaaagc ctggcttgcc tagaagtgcg aacatggttt
     1141 tcttatagct catgtagaaa aattttcttg atgatgattt tatccatcaa ttgagattga
     1201 ctgttttgct ctcctatgat ttcagctatg attgtccgat tttgcggttt tcttcttaac
     1261 tggtagttga ttttcccagt ttccatcaac tcgctaagca tatccacgac tccgccaaat
     1321 ccggtctttt tcgtaataaa acctagtgct accattcgtt catctagatt ttttgctgta
     1381 atcgtcacac cagtaatgag cgagtgctgt aatgtttggg ccagctcttc catcccttca
     1441 gcattggata cgacggaagc tgttttgtta ataggttctt cgtttatttc ttttggttct
     1501 ttcctctcca ctttcactgt ttgacgaagg acatctagtt cttgcaagag cgtttcgtat
     1561 tgttcttggg tcagcatcac gccagctact ttttcacgat taaatatata tacgccagtt
     1621 cccgcgtctc gcgcttgagc aaatacatcc atcggcgatc gtttcacttc tgtgatcgaa
     1681 gatgtgggaa cttctaattt ctttaattcc aatggatttc ctcctttact aatcgctttt
     1741 tattatagca cagactgact tgttttgatc ttttttccaa aggttttatc ggtttttgcc
     1801 taaccagtaa ttgatatcgg taagaagtct ttcgatacca aatgaaggat ggtacagata
     1861 cgttttttga tcttcgttat ggtctagtgg aaaaaaatga tcgatgatca ctagatctgc
     1921 ataccgaggc ggcttatttc gagtacatac atgtatagag atccgttttc gatgtcttag
     1981 cgttttgttc agtaccccag caagtcttct tgcttcttgg tgttcatagc gaaaaaaaaa
     2041 gacgattttc acttcaggag ggtggacatc taaataccct cgttcctgga gtgttttcag
     2101 taaaagcatc ccccactcta ctgcggggtg tgcaagtgct tgttggatcg ctagccgaaa
     2161 ttgatatagt ttatctttgg aattgggaaa tttttctaat gtttcctgat aataacgaac
     2221 ttcttctatc agccggtcat ggataccaat tacgtaacga agtcattctt ggtaaagtac
     2281 ttttgtcagc tttgatttgc tttcgctctc aaattcgcta ttcagccaaa acagcgaatc
     2341 aagcttttca gtcacaatcg tcaatttccg atagtcttgt gtctcttcta tccgtttcca
     2401 ccgcttttca gtaatctggt tacagctcaa gcgcaaaatt gctagataat ttttttcttt
     2461 tattggcaaa atctgctcct ctctaaatag caattcagga aacaattcaa agaatagtgt
     2521 tcgactgtct cctaaaatct cgatttctgc cggatccttt ctttgcttca ataattggat
     2581 gaacatctca gaaaaggact gctctccatc atctgcaaaa aaatgcggaa aatctttcaa
     2641 aaagcaaaac agcagatatc ttacttttct ttctgaacca aagatctgtg cttttttata
     2701 agaaaaagaa aggtggtact cgccaaacca ctttctcaat cgctcttgcg tagaacgatc
     2761 gtacttctgc tcgtaaggtt cattcaatag cagatttttc agtaaacaaa acgggggtga
     2821 ttggatcaga aaaacaaaca agagataaag ttcgctatat ccttgatcat aaactaactg
     2881 aacggttttc ttatgaagaa tcagctgaat gggacagtcg ctttccgcca aacgtttcag
     2941 atgccgatcc actgttttaa catcgatttt cagttcttca gctaaatcag cttttcgcca
     3001 gatcaaatct ttcattagat tgattagtga aaataaattc tgtgcatttt ttgataatag
     3061 tttttgccat ctcatcgatc atttctcacc tgatttctaa acatattgtt tgatcaaaat
     3121 gggttcactc ctaaaaagat aaaaaactcc ctttctattt atttaaatac gcaaatcttc
     3181 ctcattattt tttattttac tcaatttaat cgtattctca tttcaaaacg aatgtacttt
     3241 aaaaaaacat aataagatca ctttgcttgt aggatgtatt cccttccatt acaatgaaat
     3301 aaatagcgat tttatgagaa ggtgatgtga aaatggaaga aaaaatagaa gaatccaaac
     3361 aaaaaatcga tcagttgata taagaattag aaaaagcggt catcggcaaa cgttcaacta
     3421 tccaattgat gatcgtggcc ctattagcag gtggtcatgt cttattcgaa gatattcccg
     3481 gtgtaggtaa aacgttactg gtcaaagcac tagcaaaagc tgtacaggga accttcagca
     3541 gagtccaatg tactcctgat ctgctgccta gtgatattct tggtttttcc gtatacaatt
     3601 cacaatcaaa agagtttgaa tttcgtccgg gaccgatctt taccacgatc ttgttagcag
     3661 atgagatcaa tcgtacgact cctcgtactc agtcagcact tttagaagca atggccgaga
     3721 atcatgcaac gatcgacaac aatacatacc ctctagacaa tcattttttt gtcttagcta
     3781 cgcaaaatcc aatcgagtac gaaggaacat atcctttgcc ggaagcacag ctcgatcgtt
     3841 ttttattccg actacaaatc ggttatcctt cttttgatga cgaacttttg ctacttttgg
     3901 ataaattcga aaaagtgttg gataacttaa atgaagtatt gaacagttat gaaatagaag
     3961 aaataaaaac aaatgtggcg aatgtctttg tcagtcccga agtggcatct tatgccttgc
     4021 agttagtgtc agcaacaaga agtcatgctg ccatccagct tggcatcagt ccacgaggaa
     4081 gcctttcctt tattcaggca gcaaaagcct atgcgttgat ccatgggaga aattacgtca
     4141 cgcccaaaga tttgcaggag ttagttcctt tcgttttttc ccatcgcctg attttttacg
     4201 atcgttcatt gaaggaagac gaaaagacac aaatgatcca tgcccttgtt gcacaaatcc
     4261 caattcccgt gaggtagata atggaaaaag ccaaagatta tttcattaat tttgtgttat
     4321 ttctgatata tttcgtcatt gtcttttttg ctcttgtatt caacaattct ttagggtggt
     4381 tcttattggc cttttttact ctttatgtat catggagtgt gttttcatta agcacctttc
     4441 taaagaaact ttctgttact tgcacagtcc agccagtcgt ttatcgtaaa aaaagtatac
     4501 aggttagttt ttctatagcc aaaaagagag ctttttcttt ccctctccct cgtttacaga
     4561 tcgtctttcc agagatcttt gcagaaaaaa aacaagaaat attgatcctt acaaataaac
     4621 cgcaagaagt ccaagtactt tggcaaccaa aagagagagg gttccatgaa gcattaaccg
     4681 tcatttttac agcctatgat agtctaggac tttttagaaa aaggacaaga agagagcttt
     4741 cttttcctgt gactgttctt ccagcttttc ataagtctca agcagaagca ttgcaaagag
     4801 ttctggaaaa aaaacaacag ctgaacgcgt atcaaaaagg tcttgatttt cgagaacatc
     4861 gctcttatcg ccccggcgat tcttttaacc gaatcgattg gaaattgtct gctcatagtc
     4921 aagaatggtt ataccgcgaa tacgagtatc aagaagaaga atattctccg cttctttgct
     4981 tttggggttc acctagcttg aaatttgacc atctgctaga tttgtatttt tctttatggc
     5041 atctgagaaa agaaaagcag ccggaacttc tgatattagg cgaccgtacc tttcatggca
     5101 aagatcctgg atatacctat tttgcttctt tgaacgctgg agatgagaaa acttttttgg
     5161 cacacttgaa aaaacaggca aaaaaacaaa tcgtcctctt tattcctgcg cattctcctg
     5221 aagtctcaga agctgtcgaa acattgtcga aagatcgaac agtctatctt gtttatttcg
     5281 cagaaaaaca aatcatgatc caagaaaaag acaaggtatg ttcactgcca ggaggtgaat
     5341 ggattgacga ttaaatggga aaaatacctg cgcggcgttt actcctttat gatactaagt
     5401 gtaggtgctc cgatatttgt agcagctaat caattgcaaa acagctcccc tcttatccta
     5461 actattggta tcctatgtct tattgtctgc tgtatttcta aaaaatgggt acgattgctg
     5521 ctttctttgc cagtaatgat tggctgtttg tttgcctatt ttccttcaaa gacattttct
     5581 ttattatggg tcgtacagtt tttttcgtca gttacagagg aattcttcca tctatcccaa
     5641 ggtaaacttc attatttacc agaaaagaca gctttttttc tcatattgtt gttgatctat
     5701 ttatttatta ctttgaccgt ggattacgac tattggtatg caccctttct tgcgttgatg
     5761 gcttacttca tgatgcttac cgtttttcgg gaaaaagaac tattgtacga aatgattgcg
     5821 gtagtagctg ttttatgcgt acatttggca gcaaggcaat ttttcgccat ccgattgttg
     5881 tcgggaaacg ctcgattcca gtcgtttact acgatcctgc ttatttgttt gttcactttc
     5941 agcgccatct tgcctttata tctcccaaaa gtacaccaat ctcttgagga aacgagtgaa
     6001 ccgatccgag aatatcttaa tgatgaaggg ctttatgata cactccatga gtaccgatta
     6061 accggaagtg ccagaacggg tttcagtgaa aatgatgaac aacttggtgg tccattgtat
     6121 gataatggtg aagttgtttt tacagcgaac cagaaagtaa acggctattg gaagatagaa
     6181 aataagaatc attacaccgg ttctggatgg gtagaagatc cggatgcacc catccaacca
     6241 atccaagaat ttcctgttga tatcacaagc taccaaacag ttatggaaga aaaagaaaac
     6301 attacattga cgctaaaaaa ccaagctttc ttaccttatc cttatggaaa tactaccttg
     6361 actgattttt cattagccag tggacaaacg ctttactatt tgcctgaaag ccaacgattc
     6421 atccctgtcc caaatgaatc gacgcgattg gcgtccttac aaacagaaag attagctttt
     6481 acgagagaag aattaaaaag cagtcagtta gaggccgatg atttagaaaa tcaacagaca
     6541 aatcttcagt tgccgaaagg gttgcctcaa cgcattgagg acttggcgat cgaattgacc
     6601 aaaaatgaag caactttaat tcgtaaagtc gaagctgtcg aacagtattt gaaaacaagc
     6661 gggaattttc gctattccaa aacggatacc ggacatacgc ctaaagaacg cgattatgta
     6721 gattatttcc tcttcgattc aaaagtcggc tattgtgaca acttttcaac ttctatggtc
     6781 gtccttcttc gcacactagg catccctgct cggtggacaa aaggttttac ggatgggatt
     6841 agaacaggta cagatgagca aggaaatgag gaatacagca ttctcaatag tcatgctcat
     6901 tcttggccag aggtgtactt tcctgggttt ggctggcttc catttgaacc aaccccttca
     6961 tttgccaatc caactgtttc acaaccagcc gctgaaacaa cagaaaccgt ccaatctgct
     7021 gaagaacaac caaaagaaag cgaaaatata gcagaaacta ttaccagtga gagacctgcg
     7081 actatttcgt cagataacac gacactcaca ccgtcttata aaatcgtctt attcagcctt
     7141 ggggtgtgtt tatccgtctt gttgttcttt ctattgttat ttagaaaatc tctgcgctgg
     7201 acaagttatc ttatctatct tcattttttt gcttctacta atgcggttgc gtatcaaaaa
     7261 cttcttcacc atttggaaac actggtaccg cgcaccctcg cagaatcttt gcaggcatac
     7321 agccaacgaa tagaacaaac attcaatcta gatcaacgtt tcattcgatt aactgctcaa
     7381 tatgaagctg aaatatatgg gcgacaagca aatccggtaa aattcgataa aaaaacctat
     7441 ttagaactaa cgcatataat aagaaaacgg tatacaaaaa ggttatgaca aaagggagca
     7501 gcttcaaaaa ataagataaa attcctggag aatctacttc tggaatgccg agaaccagct
     7561 gattgagcat cgattattcg taaaaaaaga gcgtggggca aaagtaaaga acacttttgt
     7621 ctcacgctct aaaaacgtat aaacggcgga agcagaagca actccttcgg aaataagctg
     7681 aaattcacaa aaatttgaaa agcaattttc gtgaatttct tcttatttct tggagttaaa
     7741 cgcttctgtc ccagcct
//
LOCUS       DAZABD010000099         7598 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000099 DAZABD010000000
VERSION     DAZABD010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7598
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.102"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(137..427)
                     /locus_tag="ACHALG_002613"
     CDS             complement(137..427)
                     /locus_tag="ACHALG_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587938.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(545..805)
                     /locus_tag="ACHALG_002614"
     CDS             complement(545..805)
                     /locus_tag="ACHALG_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587939.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(947..1354)
                     /locus_tag="ACHALG_002615"
     CDS             complement(947..1354)
                     /locus_tag="ACHALG_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HFN2587940.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKLS"
     gene            complement(1810..2295)
                     /locus_tag="ACHALG_002616"
     CDS             complement(1810..2295)
                     /locus_tag="ACHALG_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587941.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2434..2649)
                     /locus_tag="ACHALG_002617"
     CDS             complement(2434..2649)
                     /locus_tag="ACHALG_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HFN2587942.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2873..3673
                     /locus_tag="ACHALG_002618"
     CDS             2873..3673
                     /locus_tag="ACHALG_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFN2587943.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3692..5560
                     /locus_tag="ACHALG_002619"
     CDS             3692..5560
                     /locus_tag="ACHALG_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFN2587944.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5553..6044
                     /locus_tag="ACHALG_002620"
     CDS             5553..6044
                     /locus_tag="ACHALG_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFN2587945.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6032..6586
                     /locus_tag="ACHALG_002621"
     CDS             6032..6586
                     /locus_tag="ACHALG_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFN2587946.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6604..>7598
                     /locus_tag="ACHALG_002622"
     CDS             6604..>7598
                     /locus_tag="ACHALG_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFN2587947.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2707 a         1213 c         1443 g         2235 t
ORIGIN      
        1 ttgttactat gaataacata ataagaaagt aaagtggatt gatttgagat ctttttatac
       61 ttcaaaaaag ataacaccca actttcaaaa atatgttttg gaagttggga caaacgagtt
      121 aatacatttt aatacattat tttttattca aaaaaaatag cacaatggca aagatcgtaa
      181 gagagatcat aagccccaat gaatgactaa aaagaaaagc agaatcacat gccatatcat
      241 cccatatttc ttcaagaaaa taaataagcg ggaaaaaggg aaggaatagt agaacatcga
      301 tccagcacca tttttttctt cttttgaaat aacataaaac aataaacatg aacgtgcagg
      361 gaagtaaaaa caaagcttta ctcataacta acaaaatacc agctcctttt atttttttct
      421 cccccacatt caaagagtaa caatggtatg tacttaataa tagaaaaatg gatcaacttg
      481 tatgaataac agctgaaaag atctgcacca aataaaaaac ttccagccaa aactgactgg
      541 aagtttattt ttttctgaaa tattctaaca caaatatgaa aaagatggcc gaagctaata
      601 agccgatttc gtagcaaaac aatgagatac cagagtcgta ataataaaat cctctaatct
      661 tatggataaa atagatcatc ggacaagcag ataagagtaa gtatttgtta ggccttttga
      721 ataagtaaaa gaaagcactg gtcattatga taaatggtat ccaaaacaaa agtttactta
      781 agacaatcaa atgatcaact cccatatttt ttaccaagag agatcataac atctttatcg
      841 taaaaacaaa atattttttt gctcattttg aaaatttaaa catactcgtt cttatccaaa
      901 tatataaaaa tcgcccctca ttcatcgaga ggcgataagt tctaatttaa ctcaattttt
      961 ttgtttcttt tggctgataa gctgttttag gtaataccaa tttcagcaca ccgtttttaa
     1021 aggttccagt gattttttct tcatcaacat taggaagata gaatgaacgt ttgtaagaga
     1081 ccgaatgtct ttctttccgt acatagttgc catcttcttt atcttctgta tgggactcat
     1141 gattggcgga gatcgtcaaa gtattatctg aatattcaac tgtcgtgtct tctttgtcac
     1201 agccaggcat atcagcagtt agtttgtatt ctttttcatt ttcgactaaa tctacttttg
     1261 gataacttga aacacctaaa aaatcattga atgctggact aaacacgtca ttgaaatcag
     1321 ggaacatgtc gcgaatgcta ggtacatttg ccatttttga tcatcctttc aataagtatt
     1381 ttttatttat ccctatttac gtaataactc tattttgaaa aaaatgcaag tataatggtt
     1441 agctttgtga atatttcatc taatacgata caaaggctat ctttctaatt tgaaaaaggg
     1501 atcattaaaa taataagaag aaaatatgtc aaaaggtaaa aaagtaaatt cttctatcca
     1561 aatatagaaa aattgaatct agaaaaccgc ccgacaataa atttcattaa ctcagttgac
     1621 cgaaaatgaa gtagatacca aaaatttatc aagagagcaa gtagagaagt gagtatattc
     1681 ataagagaaa tagctgtaac gaattattag gttaatgcaa atgcttattt atactccgta
     1741 taactttctc agaaaaaatg ataccaataa tgacttgcta gtactgtaaa tccgtataat
     1801 atcatctaat caaaatggtg ttccatttgc atctgtcatc gtaatcgatc cttcgggtga
     1861 cactcgataa aaaccaacgc ttccagatcc tccttgggaa cggatttgtt tgctggaagc
     1921 tgtgaacaaa taatctcgat caattttttg ataaaagcta ataacaatat cgtcactttg
     1981 taattcggaa cggtgggcaa taagaaatag aagtgctttg tcagagtttt ctaatggaag
     2041 agaaggtaca gtgctttctt gcatttcaga tgttgaagaa atgctaaacg actgaatact
     2101 tgattggcta tgttgcgaat ctacattacc agtagaagta ttagtaatgg aagtagtaga
     2161 agtactttta gttgaaccaa cctcagttgt tgaaatggct gctgaagatt ctttaacaga
     2221 attctttgtt ttatatccac aggcagaaaa aagtattaca gttcccaaca agcctgcgta
     2281 aatcccttta tgcatgatag ctcctcctaa aatagattat ctttattcag tatatcactt
     2341 aaacaaagaa aagcagatgg tttaaaatga aaaaaccttc ccaataaagg gaaggttcat
     2401 gaagataata acttttttaa taattatagc tttctaatat ctttcatcaa cttttctagt
     2461 aatcattggg aaaaatttca agcagtagat cgcacaaata cctacgacga tataaatgat
     2521 cttcgcaaaa atagttgttg aaccaccagc aatcatagct actaaatcaa gttcgaacaa
     2581 gcctaccaac agccagttca accctcccac aatcaataaa gttaatgcaa tagcatctaa
     2641 agttttcatt agaattctcc tttcttaaat gataaaacaa ttatatatca aaaagagaaa
     2701 cattgcttat ataacgctca ggaattttag caatctatgc aaaaagatga aaattttttt
     2761 tgctcttcca ttagtacaca ttttttagct gaagctgtaa atttcatgtc ttaatttttt
     2821 ttagtgagct tttatactgg atttgtaaga aaaaagaagg agaggatcac atatgtcgga
     2881 ttggttggat attgcaggaa aagtagtaat tgtaactgga ggttcctcag gaattggaag
     2941 aagtatcgtc gaaaatttgt tgaagcagaa tgcacaagta gcgaattttg atgtaactga
     3001 atgccgtata cagcatgaaa acctactatc tttaaaagtc gatgtgtctt ctaaaacgga
     3061 catagaagaa gggatatata aagtaatgaa acatttcaag acgatcgatg gtctagtcaa
     3121 taatgcagga ataaatattc cttctcttct aatagataga aatcatccaa aaagcaagta
     3181 tgaattatca gaacaagttt ttgataagat gatcgctgtc aatcaaaaaa gtgtatattt
     3241 gatgagccaa gcagttggaa gaatcttggt gcaaaaggga tcaggagtga tcgtgaatct
     3301 ttcttctgaa tcaggattgg aaggatcaga aggacagagc tgttatgctg ctacaaaagc
     3361 agcgatgaac agttttactc gttcttgggc aaaagagtta ggaaagagga atgttcgtgt
     3421 ggtaggtgtt gcaccaggga tactggaaga aaccggtttg agaacacaag aatatgaaga
     3481 agctctttct tatacgagag gtataagcgt cgaacagctg agaaacggtt acagtcgaac
     3541 aagtacgatc cctctaggaa ggagtggtaa gctgcaagaa gtggcggatc tagtctgcta
     3601 ttatctttca gaaagatcaa gttatatcac tggagtcacg actaatattt caggaggtaa
     3661 aacaagagga taaaggatag gggggacgca cgtgacgcta gtaaacagat ggtaccaatt
     3721 attgaaatta ctagtcactc acaagaaagt ttcaatccag gaagtgcaaa aagaaatcaa
     3781 aaccagtaat caaaccataa aaaaagatat tggggagttg aatgaagaaa ttgccggtat
     3841 tgcaaaaatc atccaaaaaa acaaacattt tgaacttgaa atttatgatt ttgatttttt
     3901 tgatgagatc atgcaaggaa aattgaaaac agccagtgat ttcaattcgg catccaaaag
     3961 aatcgcttat ttactaaata ggctgatcga aacagaggat ttcttacgta tggatgattt
     4021 gtctgaagaa gtcggtgtca gcagaggaac agtggtcaag gatctaaaga cattaaaaga
     4081 gatgatcaat gattttgatg taaagattac aggcacacca aataaaggac taagaataat
     4141 aggagatgag ctagaattac gactactttt attttatttt gtttctccct acttttcaga
     4201 gacgaaaaca gaacagtttc tagaagaaaa acaagcaatc aaacattttc agaagaagac
     4261 gaatgctagt aaacaagaaa tcaccctttt aacgaaagtt atcggtattt ctttgaatag
     4321 aatctctagt aactatctgt tgaagaagcc catcaatcgt tacagtgggt gttttgaata
     4381 tatccaagaa ttcaatgaac tgatcaatca gttggaagaa atctatgaac taactttgag
     4441 ccaatatgaa aaagatttta tcagttttcc tttgaacatc atcaacacaa gtataacgat
     4501 gataaggaaa gctaactctg atttgcgctt atatttcgac cccatgatga tgcgtattcg
     4561 acgattagat ctcattgatc tggatgagtc cttcttatat caagagatga aacagcacct
     4621 ctctttattg ataaatcggg taatttttcg ttatcgattg actgatttat tttatggtga
     4681 gatagaaaaa aagtatcctt tttcttacca aatcgctagc gaatgcatac aagcattaga
     4741 agctatcttg caacataaga tctctaaagt agagatcagc tacctaacgt tgtatttgga
     4801 gttagcattt cgatatcaaa aagagcattt ttcctctaaa aaagtagcca ttgttcatac
     4861 gacaggaaaa ggaacagcat taatgatcca aagacaaatc aaaaggatat tggggaggga
     4921 tatacaaata acacatgttt cagaagaaca atatgaaaaa gtagatctaa acaaatattt
     4981 cgctgttttt acaacgattc cgttaaaaaa tgtaaatgaa gagacaccgg tgatccattt
     5041 gactaatctt tttgatgact cttggttaag ggcacaatgg caaaaagcaa aaaaaaagcg
     5101 attagccggg tttcattcta ctgttttttt ctatcaaata tggaaagaaa aaaagacgta
     5161 tcaagaatat ttaaacaaat tagtcgctcc tttgatcaga ggctattatg tggatgaaga
     5221 atttgaaaaa aggatcttga aaagagaata tcagcaaagc acatttgtta acaaagaatt
     5281 tgcttttcct cacacaacca accagctatc tcaacacatt gttttatcag tagggctaat
     5341 aactgatcaa ggtttagcag aaacaggagg aaatggagtt atttttttat ttgccatacc
     5401 tgaagaaatg acggctgaag ttgagaatga attatttgag ctatacgata tcctatttga
     5461 actcataaaa atgggaatag atgagtttat tgaattagca ccgacacaag aatcattttt
     5521 aaaactaatc aaaagtaaag gagagagact tcatgaatga tatgtatcta ttcatagttt
     5581 tcgcagcttc agcttatgtg ctgcaagcag ttatcagttg gaagcaacta aagcatttca
     5641 atcaaacata tagcgaactg cgaagaaaag gaaaagtagc gattggtaaa cgacctggga
     5701 aaatccgttc aggcacgatt gttatgctag ctatagacga tcatgatcaa gttttagaag
     5761 tcagaaaaat gcaaggggtt acatctttgg caaaatttca ttcattacct gtctatgcgg
     5821 gtgaagatat ccattacatc gatcgttacc atccctcagt tcgagcggaa gatcgtttga
     5881 cacaagcagc tttagaaaat gccagagaag tatacttacg aatagaggca ggaaatgaaa
     5941 aaagtgaaca accttcattg ccttttattg ctatgctcaa taattttcgg atatggaaaa
     6001 gtacgatcaa agaaaaaagg agtgacaaac gatggatatc ataacaaaat ttgctgaggg
     6061 atttatgagg ctgttccaaa caggtgcaga aacattcata agctggatga caggaatcgt
     6121 accagttgtc ttgatgctga tggttgcaat gaatacgctg attgcttttt taggtgaaga
     6181 ccgagtaacg aaagtagcca aattatcagc gaaaaatccg cttactcgtt accttgtgtt
     6241 accgttctta tctgctttta tgttgggaaa tcctatgtca tttacaatgg ctcgtttcct
     6301 tccagaatac tacaaaccaa gctattatgc tgctcaagct caattttgtc atacgagcaa
     6361 tggtgtattc ccccatatta atcctggaga attgtttgta tggatgggga ttgctcaagg
     6421 gatcgaaacc ttagggctaa attcgatgga gctggctatc cgatacttgc ttgtgggact
     6481 gctgatgaac tttattggtg gttggataac tgattttact actggatttg tatgcagaca
     6541 gcaaggaatc gttcttagca aaaaagtaga gctttcagtt gattagagaa aggagagttt
     6601 taaatgtatg gtgatattaa gatcgtaaga ggaaacggcg ggtatggagg tcctttgatt
     6661 attaggccga caaaagataa acacaagttc atctatgtca caggtggagg tgagaggcca
     6721 atgatcgtag ataaaataga agaactgaca ggtatggagt ctatcaatgg gttcaaaaca
     6781 tcaatacctg atagtgaaat cgcgcttgca atcgttgact gtggagggac tcttagatgt
     6841 ggaatttatc ctaaaaaagg gataccaacc atcaatatcg tttccacagg aaaaagtggc
     6901 ccattagcac aatatatcac tgaagatatt tatgtttcag cagtagggtt agaacaaata
     6961 cagccactaa tggaaactga tgataacaaa gaaacagtaa atactgcatc tccagataaa
     7021 aagtacaaat atgatacttc taaaaagata accgagcaaa aagcagagca gcaaaactgg
     7081 atagcaaaaa ttggaatggg cgcgggaaaa gtagttgcta cattcaatca atcagcaaga
     7141 gaagcagtag atacgatgat ccggacagtg atccctttta tggctttcgt ttcccttttg
     7201 attggtgtga tacaaggttc tgggatcggt aatgtatttg ctaacattat ggcaccttta
     7261 gcaggaaatg cagttggatt aacaatcatt ggctttattt gttcactgcc atttttatct
     7321 cctttgcttg gtccaggggc agttattagc caagtgattg gaacattgat cggtgtagag
     7381 attgggaaag gaaatattcc tccccaatta gcattaccgg cactatttgc tatcaatact
     7441 caaaatgcat gtgattttat tccagtcggt ctaggtttgg aagaagcaaa agctgagaca
     7501 gtagaagtag gtgtaccatc agttctttat tcaagatttc ttaacggcat cccacgagtt
     7561 atcattgctt gggtagcaag ttttggatta tatagctg
//
LOCUS       DAZABD010000100         7359 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000100 DAZABD010000000
VERSION     DAZABD010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7359
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.103"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(62..286)
                     /locus_tag="ACHALG_002623"
                     /pseudo
     CDS             complement(62..286)
                     /locus_tag="ACHALG_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877485.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(840..926)
                     /locus_tag="ACHALG_002624"
                     /pseudo
     CDS             complement(840..926)
                     /locus_tag="ACHALG_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            complement(1505..2698)
                     /locus_tag="ACHALG_002625"
     CDS             complement(1505..2698)
                     /locus_tag="ACHALG_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN2587948.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDGKYLYKYVDAFGNTKYVYAWRL
                     TPTDPTPKGKREKTSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNEQRANVKKST
                     MKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKEKGFSYNTINNHKRSLKASFY
                     IAIQDDCVRKNPFDFKLSEVLENDTKEKVALTEEQEQALLSFIKTDNVYHKYYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKNGTRQVPLSR
                     ETIQAFQRVMKKRPKAEPFVIDGRSNFLFVNHKGKPKVAIDYNALFVRMVKKYNKQHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(2777..2983)
                     /locus_tag="ACHALG_002626"
     CDS             complement(2777..2983)
                     /locus_tag="ACHALG_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFN2587949.1"
                     /translation="MSNAQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEQVIDKLDAI"
     gene            complement(2976..4118)
                     /locus_tag="ACHALG_002627"
     CDS             complement(2976..4118)
                     /locus_tag="ACHALG_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFN2587950.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRFPTTDALEIIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CGGVIRRFDLAINDMCGLLDIPVLSEKYKNGGADCRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRDKKAVQAVEELLLTYNPHG
                     LVFYLITDFVQFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDIADVIESEGVFYE"
     gene            4630..5265
                     /locus_tag="ACHALG_002628"
     CDS             4630..5265
                     /locus_tag="ACHALG_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN2587951.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            5419..5496
                     /locus_tag="ACHALG_002629"
                     /pseudo
     CDS             5419..5496
                     /locus_tag="ACHALG_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345006.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            5810..5857
                     /locus_tag="ACHALG_002630"
                     /pseudo
     CDS             5810..5857
                     /locus_tag="ACHALG_002630"
                     /inference="COORDINATES: protein motif:HMM:NF011335.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5873..>7359
                     /locus_tag="ACHALG_002631"
     CDS             5873..>7359
                     /locus_tag="ACHALG_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFN2587952.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYTSGKLLEALELEQEESIRFHNCSLFPVYH
                     GSAKNNIGIDNLIEVITNKFYSSTHRGQSELCGKVFKIEYSEKRQRLAYIRLYSGVLH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIEIKEPTVIYMERPLKKAEYTIHIEVPPNPFWASIGLSVA
                     QLPLGSGVQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVT"
BASE COUNT         2250 a         1344 c         1373 g         2392 t
ORIGIN      
        1 taggtatttt tattatatat agatttaaca tatgtcggtc ggttgattga tatagctaaa
       61 gttataactc aaccgaagcc gattcatcta cacgaataaa gttgcgagtg ttctcggcta
      121 attcataaaa attgttatgt gttccatata gcaatactgg aataacttta ttttcttgaa
      181 tctcataagc aactctatac acggtgaccg catacttaaa tccattagac caattacctg
      241 ataaacctcc agttttttgc tcttacttaa atggatcaat ggcaatttca tcacaaacct
      301 atagaggatg agtgacttct gcctaattac gttaaaaaat attgaaagta actctatgta
      361 gcaaaaaata tctagaattt catttcctca aaactgatcg atattgtatc taaaaatata
      421 tactacctaa aataggtagt atatattaaa atatgtgctt ttgttagaaa aatcaataca
      481 taaattatca tatctgtata tgtcatttag gtatttcagt tatgttataa tagttttgct
      541 gtctgctacc ccggcctaat ctggcaaggg gaggaggaga tataatgaaa tacctttttg
      601 attttgtttt tgcaatcaag ccacttgcag atgtagtgtg cagttacctc tgcaaatggc
      661 ttgattgcaa aattgccaaa aaagacagta agcctacccg ttaattctat aatatagaaa
      721 cgtaaaaaaa accccatcag cctgcacgct gatggggttt ggtgatataa tgaaatacct
      781 ctttgttact cataattata ccataattag tcacgaatgc aaccaataga aaaaactact
      841 tagaacggta gatcatcgct aataacaatg cattgtccac caaatggatt ttgtgaagca
      901 ttaaatacat tgttattaat tgtcgctgct tttaactagg cctctcccct tctattataa
      961 aagaagatat atatcatttc ttaattacct actaccttaa accaaaattt aagtactacc
     1021 taatttaagt agtacttaaa caatctaaaa aatagttatc ccaattgtta tgcttttata
     1081 atacgattat ttgtaacaaa atgaatatta tataattaaa cactaccata tttaggtagt
     1141 gtttaattat tagcaaaaca aaaaatattt attctattct cttaacttca actaagttat
     1201 gctgcacacc tttatataaa tcatatgcaa ctcattagct agacaaacta caagcatgta
     1261 gttttatctg ttaattatgt actacctaaa taaagtagtt tataattagc aagtatatat
     1321 atttaaaaat ttcatttaaa ttatatatgc ctttttgact atctttttat atcttctcaa
     1381 atgtccgcaa agccttattt tatcggctct acgggcattt tgcttttgtg gtaaacctca
     1441 catatctagg tctatctttt tatattttcg ctatcaaacg tggttaaaat cgtggtaaat
     1501 acttctatgc gattagacgc tgaacctctg attttgcggt atctatggac gcatgagcat
     1561 accagttcat tgtgatactg atgtttgaat gtcccatgat atactgtaaa tctttcgggt
     1621 tcatgttctt gcttgccagc cttgtgcaga acgtatggcg tagcgtatgc ggtgtgatat
     1681 gtggcaaggg attgtccttg tgttgcttgt tgtatttctt taccatacgg acaaataagg
     1741 cattgtaatc aatcgcaact ttgggcttgc ctttatgatt gacaaacaga aaattgctcc
     1801 gtccgtctat cacaaatggt tctgcctttg ggcgtttttt cataacccgt tgaaatgcct
     1861 gtattgtttc tctgcttaat ggcacttgcc ttgttccgtt ctttgtttta ggcgtttcaa
     1921 tataatagcc ctgttccttg ctctttaata actggtggtc gataatcaca acttcattct
     1981 tgaaatcaat atcggctact gtcagtccgc acagttccga gatacgaagt ccagtcttta
     2041 acagtatcag cacatcatca taatacttat gatacacgtt gtccgtctta ataaatgaca
     2101 gtagggcttg ttcttgttcc tctgtcaatg ccactttctc tttggtatca ttttctagga
     2161 cttcacttaa cttgaaatca aaagggttct tccttacaca atcgtcttgt atggcgatat
     2221 agaatgacgc ttttaacgag cgtttatggt tgttaatagt attgtaggaa aagcctttct
     2281 ctttcatgcg taacgcccat tccttagcgt cagagggttt tatcgtatca atgctcctag
     2341 cacccaactt gtcctctttc aataaccgca tgagctgttc ccgttgtttc attgtgcttt
     2401 tcttcacgtt tgccctctgt tcgttctgtt tggcgtagag ttggcaaagc gtcattttct
     2461 tgcctgtgct gtcgataccg tcctcaatat cccgtcttat ctgctgttcc agttcacgaa
     2521 gtgaggtttt ttcccgtttt ccctttggtg tcgggtctgt gggtgtcaat ctccaagcat
     2581 acacatattt tgtatttcca aatgcgtcta catatttata caagtatttt ccgtctgttc
     2641 gttggctctc tccagtatgc aggatacgtc ctttgttatc ccgtcttttt tctttcatgg
     2701 tgtctgctcc tttccttgtt ggaaagagcc ttgatatgac ttgtgttcat cataacacat
     2761 acaaggctca tttgcattag attgcgtcca atttatcaat aacctgttca aactgtcgtc
     2821 gtttaatctg tatgcggttg ccattcataa tgagccagcc agcgtccttg ttttcctctg
     2881 ccaagcgtct taacttgttt tctccgatac ggaaatactt tgacgcttct tcaatggtaa
     2941 gggtgtattt ttcccatacg ggaatatcct gtgcattact cataaaacac cccctcactt
     3001 tcaattacgt ctgctatgtc cgtacacatt tgttccacaa gcatttcctg tttttcagaa
     3061 aggtgggtac attcatcaat cattttcatg tagtttgagc ctgtcagcct gtcgatttcc
     3121 tctatcatca cgattgacgg catgacctgt ctttccagcc attgcagagt gcgttccata
     3181 ttgacaggta cggggttcat ttcaaaaggt aaactatcat gggaaatgaa tatctcccac
     3241 agcggatagt cgggaaattg cacaaaatca gtaataaggt aaaacaccag cccatgaggg
     3301 ttatacgtca ataacagttc ctcaactgcc tgtactgcct ttttgtcacg cagacgaatt
     3361 tcaaaacggt tgataatatc cgtatgtttc tttttagttg cctgttcctt ttgtttttca
     3421 tacaggcaga aatattttgt gcttgctttt gagccgatat aaagggtgct tgctaaattg
     3481 cggttttttc cgcttagttt accgccttgc acattttcat agtttttaca gcgacaatcc
     3541 gcaccgccat ttttgtattt ttcagataaa acgggaatat ccagcagacc gcacatatcg
     3601 ttgattgcca agtcaaaacg tcggataaca ccgccacagt caagacagcg gttcaagaat
     3661 gaataccaat ccctgttctg tgcctgtaaa acatattcca tattgcgtga gcctgcccct
     3721 tttaattcca aaaatacgcc catgtgttca tcatcagaag ccataacctt gatttctccg
     3781 taggcgtaat gctctttata gccataatat ccatagtcat aatggataaa ataatctgct
     3841 ttcatgccaa gcacctttcg gataatttcc aatgcgtcag tcgttggaaa acggacagac
     3901 agataatcaa cgagcagata aaaaggctta tcgcagatat agtaattcag acaacgcagg
     3961 ataatatttt gtgtttcctt gctggcgttg gcttttccgt tctcaaagcg gttgatacta
     4021 tatcttgata catgggtaag acttgcaagt tctccttgtg aaagaccgct tgcaagacgt
     4081 ttattcttca tttcaagtgc aaattctaaa ttgttcattg tattcctcca atcccacaat
     4141 agccttgaaa tacctgtaaa ccactattca aaagaatgtg aaattactat cttcttaaaa
     4201 ctcctcatta ctccttacag ctttggggtt gtatggtttt tgacttgtct tttgtgaaat
     4261 agtgcccccc tgttagataa cgggggcaca gacaaggctc gcaggctcgc cagccgacaa
     4321 agcatttctt ccccttgaca caaggtgtca tggcggtaag cctgagaggc ttccgccacc
     4381 gtgtcagaaa tactttgtca gcaagttctg ttttgccttt tctaacaagt tccatttcaa
     4441 ccgatattca tatctacgga tttaatcaga taggtatttc tttttgaatg tttttatatt
     4501 tcattctatt gtgcttattc gattacaaga acatagtaat tcttatatgc ttatttgtca
     4561 atatcttttt ttgcctttac aaatcatatt aagcatgata gacttataat agcgaagata
     4621 taacgagata tggagggtta tgcaatgaaa ataagccatt taggaaataa tatacagacc
     4681 ataagaaaat ttaggggaat gaaacaacag gaacttgcgg acaaaatcgg tatcaatatg
     4741 cagagccttt ccaagattga aagagggttg aactatcctg cctatgaaac gctggaaaag
     4801 ataatggaag tgctggacgt tacgcccaat gaattgttgt cgggagaatg gaagtatgtc
     4861 aatcaatctg aaaaggaagt ctgtcagttt ttaagagccg aagaacgtct aaatgcagaa
     4921 ctgaaacatg gacactatga taacttcttt gacagcgagg aagaatggct ggaatatgag
     4981 ttagaaaagt tacgggaata tattacagac tatatcaacg ggaaaagcat tgaagcgtcc
     5041 gacctttacc caatcaaaga atttatccag catttgaagt ttcaaaaatt attagaccgc
     5101 tatgatgatt tatacagtat ggatatgttt ggggaaagca tagagggaca taagtataag
     5161 acaccttatc aagtcgtgaa aatgataaac ccgaactcaa aagaagatat ggaactgtta
     5221 cgagaagtat taaggaataa caattttgat gatgaggacg agtaactttt ttcctcttgc
     5281 gagaagatac agcaaacaaa aagcccgatc aagagtgcaa agcaccacca atggtggcaa
     5341 agctcttgac agggcttttt cttttctgtt gtgggcaatc aagaggaaga aagggataaa
     5401 atctgatttg tggtaaatca ggcaaaggca acgcaggtat ctcaatttga tttggtactt
     5461 gaaaagaacg ggagtaattg gaagattata gaataacaaa tattggtaca ttattacagc
     5521 tattttgtaa tcacgtactc tctttgataa aaaattggag attcctttac aaatatgctc
     5581 ttacgtgcta ttatttaagt atctatttaa aaggagttaa taaatatgcg gcaaggtatt
     5641 cttaaataaa ctgtcaattt gatagtggga acaaataatt ggatgtcctt ttttaggagg
     5701 gcttagtttt ttgtacccag tttaagaata cctttatcat gtgattctaa agtatccgga
     5761 gaatatctgt atgctttgta tgcctatggt tatgcataaa aatcccagtg ataagagtat
     5821 ttatcactgg gatttttatg cccttttggg cttttgaatg gaggaaaatc acatgaaaat
     5881 tattaatatt ggagttttag ctcatgttga tgcgggaaaa actaccttaa cagaaagctt
     5941 attatataac agtggagcga ttacagaatt aggaagcgtg gacaaaggta caacgaggac
     6001 ggataatacg cttttagaac gtcagagagg aattacaatt cagacaggaa taacctcttt
     6061 tcagtgggaa aatacgaagg tgaacatcat agacacgcca ggacatatgg atttcttagc
     6121 agaagtatat cgttcattat cagttttaga tggggcaatt ctactgattt ctgcaaaaga
     6181 tggcgtacaa gcacaaactc gtatattatt tcatgcactt aggaaaatgg ggattcccac
     6241 aatctttttt atcaataaga ttgaccaaaa tggaattgat ttatcaacgg tttatcagga
     6301 tattaaagag aaactttctg ccgaaattgt aatcaaacag aaggtagaac tgtatcctaa
     6361 tatgtgtgtg acgaacttta ccgaatctga acaatgggat acggtaatag agggaaacga
     6421 tgacctttta gagaaatata cgtctgggaa attattggaa gcattagaac tcgaacaaga
     6481 ggaaagcata agatttcata attgttccct gttccctgtt tatcacggaa gtgcaaaaaa
     6541 caatataggg attgataacc ttatagaagt gattacgaat aaattttatt catcaacaca
     6601 tcgaggtcag tctgaacttt gcggaaaagt tttcaaaatt gagtattcgg aaaaaagaca
     6661 gcgtcttgca tatatacgtc tttatagtgg cgtactgcat ttgcgagatt cggttagaat
     6721 atcggaaaag gaaaaaataa aaattacaga aatgtatact tcaataaatg gtgaattatg
     6781 taaaatcgat aaggcttatt ccggggaaat tgttattttg caaaatgagt ttttgaagtt
     6841 aaatagtgtt cttggagata caaaactatt gccacagaga aaaaagattg aaaatccgca
     6901 ccctctacta caaacaactg ttgaaccgag taaacctgaa cagagagaaa tgttgcttga
     6961 tgcccttttg gaaatctcag atagtgatcc gcttctacga tattacgtgg attctacgac
     7021 acatgaaatt atactttctt tcttagggaa agtacaaatg gaagtgatta gtgcactgtt
     7081 gcaagaaaag tatcatgtgg agatagaaat aaaagagcct acagtcattt atatggaaag
     7141 accgttaaaa aaagcagagt ataccattca catcgaagtt ccaccgaatc ctttctgggc
     7201 ttccattggt ctatctgtag cacagcttcc attagggagc ggagtacagt atgagagctc
     7261 ggtttctctt ggatacttaa atcaatcgtt tcaaaatgca gttatggagg ggatacgcta
     7321 tggctgtgaa caaggattgt atggttggaa tgtgacgga
//
LOCUS       DAZABD010000101         7168 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000101 DAZABD010000000
VERSION     DAZABD010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7168
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.105"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..531
                     /locus_tag="ACHALG_002632"
     CDS             <1..531
                     /locus_tag="ACHALG_002632"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFN2587953.1"
                     /translation="RSYSKEFKQKIVAEYLQTGYGFSYLAAKYNIPSKTTVNKWVIRY
                     TEGKENETYSPKSEVYNMTGVKKSYEEKLKIVEDYIANRLTYLEAAEKNHVSYSNIYS
                     WVNKYKKHGPIGLEDNRGRGKPTEIQTTEERLNAEVETLKARNKWLEMENDALKKRRK
                     IAGNHKSQGLDKKWNI"
     gene            519..1379
                     /locus_tag="ACHALG_002633"
     CDS             519..1379
                     /locus_tag="ACHALG_002633"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFN2587954.1"
                     /translation="MEYLTIEELKHKYPVIHLCDILGIAKSSYYKWLKREPSETELKR
                     LKLMRAIKGIHEAFGGIYGYRRMTIFLNFFRRAKVNHKCVHRLMRIMGITAVIRRKRR
                     NYVPHKAVHVAENILNRDFHAERPMEKLLTDVTEFRLTNGTKRYLSAIYDLGSKKIVA
                     YKTSHRNDNPLVLDTLKQILGDVKPETTLIHSDRGSQYTSHAFNKMIKDHQIIHSMSR
                     VSKCIDNGPMEGFWGTLKVEMFNLDTFDRPAYLDRKIKAYIAFFNNERVTLDMGLAIP
                     TEEKILQMIA"
     gene            complement(1464..4388)
                     /locus_tag="ACHALG_002634"
     CDS             complement(1464..4388)
                     /locus_tag="ACHALG_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HFN2587955.1"
                     /translation="MKIARGRELLTPEQRQAFMQIPEDEWILGTYFTFSKRDLEIVNK
                     RRREENRLGFAVQLAVLRYPGWPYTHIKSIPDSVIQYISKQIGVSPSSLDHYPQRENT
                     LWDHLKEIRSEYDFVTFTLSEYRMTFKYLHQLALENGDAIHLLHECIDFLRKNKIILP
                     AITTLERMVWEARAMAEKKLFNTVSKSLTNEQKEKLEGIITSQHPSESNKTILGWLKE
                     PPGHPSPETFLKIIERLEYIRGMDLETVQISHLHRNRLLQLSRLGSRYEPYAFRDFQE
                     NKRYSILTIYLLQLTQELTDKAFEIHDRQILSLLSKGRKAQEEIQKQNGKKLNEKVIH
                     FTNIGQALIKAREEKLDVFKVLESVIEWNTFVSSVEEAQELARPADYDYLDLLQKRFY
                     SLRKYTPTLLRVLEFHSTKANEPLLQAVEIIRGMNESGKRKVPDDSPVDFISKRWKRH
                     LYEDDGTTINRHYYEMAVLTELREHVRAGDVSIVGSRQYRDFEEYLFSEDTWNQSKGN
                     TRLSVSLSFEDYITERTSSFNERLKWLAANSNKLDGVSLEKGKLSLARLEKDVPEEAK
                     KFSASLYQMLPRIKLTDLLMDVAHITGFHEQFTHASNNRKPDKEETIIIMAALLGMGM
                     NIGLSKMAEATPGLTYKQLANVSQWRMYEDAMNKAQAILVNFHHKLQLPFYWGDGTTS
                     SSDGMRMQLGVSSLHADANPHYGTGKGATIYRFTSDQFSSYYTKIIHTNSRDAIHVLD
                     GLLHHETDLNIEEHYTDTAGYTDQIFGLTHLLGFKFAPRIRDLSDSKLFTIDKASEYP
                     KLEAILRGQINTKVIKENYEDVLRLAHSIREGTVSASLIMGKLGSYSRQNSLATALRE
                     MGRIEKTIFILNYISDESLRRKIQRGLNKGEAMNGLARAIFFGKQGELRERTIQHQLQ
                     RASALNIIINAISIWNTLHLTTAVEYKKRTGSFNEDLLHHMSPLGWEHINLLGEYHFN
                     SE"
     gene            4534..5109
                     /locus_tag="ACHALG_002635"
     CDS             4534..5109
                     /locus_tag="ACHALG_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFN2587956.1"
                     /translation="MRKIGYIRVSSTNQNPSRQFQQLNEIGMDIIYEEKVSGATKDRE
                     QLQKVLDDLQEDDIIYVTDLTRITRSTQDLFELIDNIRDKKASLKSLKDTWLDLSEDN
                     PYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKL
                     YKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            5323..6018
                     /gene="vanR-A"
                     /locus_tag="ACHALG_002636"
     CDS             5323..6018
                     /gene="vanR-A"
                     /locus_tag="ACHALG_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFN2587957.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            6057..7151
                     /gene="vanS"
                     /locus_tag="ACHALG_002637"
     CDS             6057..7151
                     /gene="vanS"
                     /locus_tag="ACHALG_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000791309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS"
                     /protein_id="HFN2587958.1"
                     /translation="MYIVAIVVVAIVFVLYIRSMIRGKLGDWILSILENKYDLNHLDA
                     MKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKYFDEINTGIDVLIQNEDKQIE
                     LSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVMYLAHDIKTPLTSIIGYLSLL
                     DEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRYNLQTITLTKTHIDLYYMLVQ
                     MTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFNNILKNAAAYSEDNSIIDITA
                     GLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSSDTGGAGLGLAIAKEIIVQHG
                     GQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT         2274 a         1375 c         1401 g         2118 t
ORIGIN      
        1 cgcagttact cgaaagaatt caagcagaaa attgtagctg aatacctaca gacgggttac
       61 ggtttttcat acttagccgc taaatataac attccatcta aaacaacggt aaacaaatgg
      121 gtaattcgat atactgaagg aaaagagaat gagacatatt ctccgaaatc tgaggtgtac
      181 aacatgacgg gtgttaaaaa atcatatgaa gaaaaattaa aaatcgtaga agattacata
      241 gccaatcgcc tgacttatct ggaagcagcg gagaaaaacc atgtaagtta cagtaacatt
      301 tattcctggg tgaataaata caaaaagcat ggaccgatag gtctggaaga caaccggggc
      361 cgcgggaagc ccacggaaat tcaaacaacg gaagaacgtt taaacgcaga agtagagaca
      421 ctcaaagccc ggaacaaatg gctagaaatg gagaacgatg cgttaaaaaa gcgccgaaaa
      481 atcgctggga atcacaaatc acaggggtta gacaagaagt ggaatattta actattgaag
      541 aattaaagca taaatatccc gttatccatc tttgtgacat actgggtatc gccaaatcca
      601 gctactataa gtggttaaaa cgggaaccct cagaaacaga attaaaacgc ctgaaactga
      661 tgcgggcgat caaaggaatc cacgaggcat tcggtgggat ttacggctac cgaagaatga
      721 ccatctttct caactttttt agaagagcga aagtgaatca taagtgtgta caccgcctca
      781 tgagaatcat ggggatcaca gccgtcatcc gtcgcaaaag aaggaactac gtgccacaca
      841 aagccgtaca tgtggctgaa aacatcttaa accgtgattt ccacgctgaa agacccatgg
      901 aaaagttatt gacggatgtc acggaattcc ggttgaccaa tgggacaaaa cgttacctga
      961 gcgctattta tgacctcggg tcaaagaaaa tcgtggctta taaaaccagt caccgcaatg
     1021 acaacccgtt agtactggat acactaaagc agattttggg tgatgtaaag cctgaaacca
     1081 cactgattca tagcgaccgc ggttcccagt acacctccca tgcctttaac aagatgatta
     1141 aagatcacca gataatccat agtatgtcac gcgtctcgaa atgtattgac aacggcccta
     1201 tggaaggctt ttggggtacc ctcaaggtgg agatgtttaa cctggatacg tttgacagac
     1261 cagcgtactt agaccggaaa atcaaggcat acatcgcgtt cttcaacaat gaacgcgtta
     1321 ctttggatat gggattagca attcctacgg aagagaagat tctacaaatg attgcataaa
     1381 cgctacaaaa aaagaccaca catttttaca tgcatggtct aaaatacttt gtttttttac
     1441 ctgtctactt gacagggggc agttcactct gagttaaaat ggtattctcc tagtaaatta
     1501 atatgttccc aacctaaggg cgacatatgg tgtaacaaat cttcattaaa gctacctgtc
     1561 cgttttttat attcaactgc tgttgttagg tggagagtat tccaaatact tatagcattg
     1621 ataattatgt ttaaagcact ggctctttgc aattgatgct gtatggtgcg ttctctaagc
     1681 tcaccttgtt ttccgaagaa aatagctctt gccaatccat tcatggcttc tcctttattc
     1741 aatcctcttt gtatttttct tcttaatgat tcatccgata tataattcaa aataaagatc
     1801 gttttttcta ttcggcccat ctcacgtaag gctgtagcta agctgttttg tcttgaatag
     1861 gaacctagct tccccataat aagggatgct gaaactgttc cctcccttat agaatgagct
     1921 aatcgcaaaa catcctcata attttcttta atgacctttg tatttatttg tccacgtaaa
     1981 atggcttcta gttttggata ctcacttgct ttatctatcg taaataattt tgagtccgat
     2041 aaatccctta ttcttggggc aaatttaaat cctaataaat gagtcagtcc gaatatttgg
     2101 tcagtgtaac cggcagtgtc tgtataatgt tcctctatgt ttagatccgt ctcatgatgt
     2161 aacaaaccat ccaaaacatg aatcgcatct cttgaattag tatgaataat ctttgtgtag
     2221 taagaagaga attgatcact tgtaaatcgg tagatggtgg ctccttttcc agttccataa
     2281 tgtggatttg catctgcatg tagtgatgaa acacctagct gcattctcat accatctgac
     2341 gaagatgttg taccgtcgcc ccaatagaaa ggcaattgta atttatgatg aaagtttact
     2401 aatatggctt gggctttatt catggcatct tcatacatgc gccattgaga tacattggct
     2461 agttgcttat atgtaagtcc gggtgtggct tcggccatct tgctcaagcc aatattcatt
     2521 cccattccta aaagggcagc catgataatg attgtttctt ccttatctgg ttttcgatta
     2581 ttggaagcat gagtgaattg ctcatgaaat cctgttatat gggccacatc catgagtaaa
     2641 tcagttaatt ttattcttgg tagcatctga taaaggcttg cactaaattt ttttgcttct
     2701 tctggaacat ctttttctaa gcgtgcaagt gatagctttc ctttttcaag agaaacccca
     2761 tctaacttat tggaattggc agctaaccac tttaaccttt cattaaagct gctggttctc
     2821 tccgttatat aatcttcgaa tgataaacta actgataatc tcgtattccc cttcgattga
     2881 ttccatgtat cttccgaaaa caaatattcc tcaaaatccc tatattgtct gctgccaaca
     2941 atggaaacat ctcctgcccg aacatgctcc cgaagttctg ttaaaacagc catttcatag
     3001 taatgacgat taattgttgt accatcatcc tcgtataaat gtcttttcca tcgttttgaa
     3061 ataaaatcca caggtgagtc atcaggcact tttcgctttc cagattcgtt cattcctcgg
     3121 ataatctcaa cagcttgtaa aagtggctca tttgcctttg tagaatgaaa ttccaatact
     3181 cttaatagcg ttggcgtata ttttcttagt gaataaaacc gtttttgcag taagtctaaa
     3241 taatcatagt cggcaggacg tgcaagttcc tgagcctctt ctactgaaga gacaaaggta
     3301 ttccattcaa taaccgattc taaaacctta aaaacgtcta atttttcctc tcttgcttta
     3361 attaatgctt gtccgatgtt cgtaaagtgt ataactttct catttagctt tttaccgttt
     3421 tgtttctgga tttcctcttg agccttacga ccttttgata acaaactaag tatttgccta
     3481 tcatgaattt caaacgcttt atccgttagc tcctgagtaa gttgtaataa atagatggtt
     3541 aatatcgaat aacgtttatt ttcttgaaag tcacggaatg catacggctc gtatcttgag
     3601 cctaagcgag acagctgcaa caggcggtta cggtgcaaat gactaatttg cactgtttct
     3661 aaatccattc ctcgtatgta ttcgagtcgt tctattattt ttagaaaagt ttcgggtgaa
     3721 ggatgacccg gtggctcttt taaccaaccc aatatcgttt tattggattc ggatggatgc
     3781 tgcgaggtaa taatcccttc aagcttttct ttttgctcat ttgttagaga tttactaacc
     3841 gtattaaata gcttcttttc agccattgcc cttgcttccc acaccattct ttcaagtgta
     3901 gtgatagcag gcagtataat tttgtttttt cttagaaaat ctatgcattc atgcagtaga
     3961 tgaatggcat caccattttc caaagctaat tgatgaaggt acttaaatgt cattcgatat
     4021 tcactcaggg taaaagttac aaagtcgtat tcacttcgaa tttctttcaa atgatcccaa
     4081 agtgtatttt ccctttgagg ataatgatca agcgaggatg gactaacacc aatctgtttc
     4141 gatatatatt gtatgaccga atctgggatg cttttgatat gagtgtatgg ccaaccggga
     4201 taccgaagaa cagctaattg aacagcaaat cctaaacggt tttcttccct ccttcgctta
     4261 ttaactattt ctaaatcccg tttggaaaaa gtgaagtagg tccccagtat ccattcatct
     4321 tcagggattt gcataaaagc ctgtctctgt tccggtgtaa gcaattctct acctctcgca
     4381 attttcattc agtatcattc catttctgta ttttcaattt attagttcaa ttatatatca
     4441 atagagtgta ctctattgat acaaatgtag tagactgata aaatcatagt taagagcgtc
     4501 tcataagact tgtctcaaaa atgaggtgat attttgcgga aaatcggtta tattcgtgtc
     4561 agttcgacta accagaatcc ttcaagacaa tttcagcagt tgaacgagat cggaatggat
     4621 attatatatg aagagaaagt ttcaggagca acaaaggatc gcgagcaact tcaaaaagtg
     4681 ttagacgatt tacaggaaga tgacatcatt tatgttacag acttaactcg aatcactcgt
     4741 agtacacaag atctatttga attaatcgat aacatacgag ataaaaaggc aagtttaaaa
     4801 tcactaaaag atacatggct tgatttatca gaagataatc catacagcca attcttaatt
     4861 actgtaatgg ctggtgttaa ccaattagag cgagatctta ttcggatgag acaacgtgaa
     4921 gggattgaat tggctaagaa agaaggaaag tttaaaggtc gattaaagaa gtatcataaa
     4981 aatcacgcag gaatgaatta tgcggtaaag ctatataaag aaggaaatat gactgtaaat
     5041 caaatttgtg aaattactaa tgtatctagg gcttcattat acaggaaatt atcagaagtg
     5101 aataattagc cattctgtat tccgctaatg ggcaatattt ttaaagaaga aaaggaaact
     5161 ataaaatatt aacagcctcc tagcgatgcc gaaaagccct ttgataaaaa aagaatcatc
     5221 atcttaagaa attcttagtc atttattatg taaatgctta taaattcggc cctataatct
     5281 gataaattat taagggcaaa cttatgtgaa agggtgataa ctatgagcga taaaatactt
     5341 attgtggatg atgaacatga aattgccgat ttggttgaat tatacttaaa aaacgagaat
     5401 tatacggttt tcaaatacta taccgccaaa gaagcattgg aatgtataga caagtctgag
     5461 attgaccttg ccatattgga catcatgctt cccggcacaa gcggccttac tatctgtcaa
     5521 aaaataaggg acaagcacac ctatccgatt atcatgctga ccgggaaaga tacagaggta
     5581 gataaaatta cagggttaac aatcggcgcg gatgattata taacgaagcc ctttcgccca
     5641 ctggagttaa ttgctcgggt aaaggcccag ttgcgccgat acaaaaaatt cagtggagta
     5701 aaggagcaga acgaaaatgt tatcgtccac tccggccttg tcattaatgt taacacccat
     5761 gagtgttatc tgaacgagaa gcagttatcc cttactccca ccgagttttc aatactgcga
     5821 atcctctgtg aaaacaaggg gaatgtggtt agctccgagc tgctatttca tgagatatgg
     5881 ggcgacgaat atttcagcaa gagcaacaac accatcaccg tgcatatccg gcatttgcgc
     5941 gaaaaaatga acgacaccat tgataatccg aaatatataa aaacggtatg gggggttggt
     6001 tataaaattg aaaaataaaa aaaaacgact attccaaact agaacgaaaa ctttacatgt
     6061 atatcgttgc aattgttgtg gtagcaattg tattcgtgtt gtatattcgt tcaatgatcc
     6121 gagggaaact tggggattgg atcttaagta ttttggaaaa caaatatgac ttaaatcacc
     6181 tggacgcgat gaaattatat caatattcca tacggaacaa tatagatatc tttatttatg
     6241 tggcgattgt cattagtatt cttattctat gtcgcgtcat gctttcaaaa ttcgcaaaat
     6301 actttgacga gataaatacc ggcattgatg tacttattca gaacgaagat aaacaaattg
     6361 agctttctgc ggaaatggat gttatggaac aaaagctcaa cacattaaaa cggactctgg
     6421 aaaagcgaga gcaggatgca aagctggccg aacaaagaaa aaatgacgtt gttatgtact
     6481 tggcgcacga tattaaaacg ccccttacat ccattatcgg ttatttgagc ctgcttgacg
     6541 aggctccaga catgccggta gatcaaaagg caaagtatgt gcatatcacg ttggacaaag
     6601 cgtatcgact cgaacagcta atcgacgagt tttttgagat tacacggtat aacctacaaa
     6661 cgataacgct aacaaaaacg cacatagacc tatactatat gctggtgcag atgaccgatg
     6721 aattttatcc tcagctttcc gcacatggaa aacaggcggt tattcacgcc cccgaggatc
     6781 tgaccgtgtc cggcgaccct gataaactcg cgagagtctt taacaacatt ttgaaaaacg
     6841 ccgctgcata cagtgaggat aacagcatca ttgacattac cgcgggcctc tccggggatg
     6901 tggtgtcaat cgaattcaag aacactggaa gcatcccaaa agataagcta gctgccatat
     6961 ttgaaaagtt ctataggctg gacaatgctc gttcttccga tacgggtggc gcgggacttg
     7021 gattggcgat tgcaaaagaa attattgttc agcatggagg gcagatttac gcggaaagca
     7081 atgataacta tacgacgttt agggtagagc ttccagcgat gccagacttg gttgataaaa
     7141 ggaggtccta agagatgtat ataatttt
//
LOCUS       DAZABD010000102         6905 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000102 DAZABD010000000
VERSION     DAZABD010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6905
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.106"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..173
                     /locus_tag="ACHALG_002638"
     CDS             <1..173
                     /locus_tag="ACHALG_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HFN2587959.1"
                     /translation="RNIFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLF
                     ATLLNYSIYFLN"
     gene            complement(281..1507)
                     /locus_tag="ACHALG_002639"
     CDS             complement(281..1507)
                     /locus_tag="ACHALG_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFN2587960.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(2261..2461)
                     /locus_tag="ACHALG_002640"
     CDS             complement(2261..2461)
                     /locus_tag="ACHALG_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFN2587961.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(2595..3203)
                     /locus_tag="ACHALG_002641"
     CDS             complement(2595..3203)
                     /locus_tag="ACHALG_002641"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFN2587962.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNAAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(3288..3758)
                     /locus_tag="ACHALG_002642"
     CDS             complement(3288..3758)
                     /locus_tag="ACHALG_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFN2587963.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(3806..4720)
                     /locus_tag="ACHALG_002643"
     CDS             complement(3806..4720)
                     /locus_tag="ACHALG_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFN2587964.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKNVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            4866..5669
                     /locus_tag="ACHALG_002644"
     CDS             4866..5669
                     /locus_tag="ACHALG_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HFN2587965.1"
                     /translation="MTTKQLIISSFIRFKDLKETRNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKELR
                     SFLSKASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            5847..6794
                     /locus_tag="ACHALG_002645"
     CDS             5847..6794
                     /locus_tag="ACHALG_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFN2587966.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
BASE COUNT         2149 a         1368 c         1047 g         2341 t
ORIGIN      
        1 taagaaatat tttcattgca gcaatcgata ttacactttt agttattttc ttgtcagtgc
       61 ttatgtatca gttgtggcta catcatcgcc tggcaatgtg gctcatgatt ccttattatt
      121 tatgggtact attcgcaact ctattgaatt attcaattta ttttttaaac taagcgctaa
      181 aaagagacgt cctgtaattt aacacataga aaaagtgcca cctaagttag attttttgtc
      241 taatttaggt ggcactcttt attaaccaaa ctattttttt ctagtaccca cttgttgttc
      301 catactcatt attctggtca taaccataac cattttgttc ctgtccagcg taaggatcat
      361 aactttgagt atcttgactt ggtgtatagt actgacttgt atcttctgtt tgctgataat
      421 aggtattcgg atcttggtac tgatcatatg tgttttctga ataggaagaa taatcggtgt
      481 acccttcact attcgatcca tcaccgtttg tcgaatcgcc atcttccgaa tcatcaaaga
      541 acatcattga agcattctca tttttcaaat taggtaatgt atcgcttgtt tttatattta
      601 attgtttctt cagttcattt tggatcgaaa gaagatcatt ttttcctaaa atttgatagt
      661 agatatcatt gatcatttgt ccttcgcctt gcaactggtc ttgtttgatt gtttcaaacg
      721 caggggtgta gttcgtcgct acatcaagca tgtcatccca atctaagtcc gtcgtcacat
      781 tgttctcaac tgcttttaaa atttttctat aattgcttac gccatctaaa cttaaaactt
      841 tattgacgat tttcgtcact acttcccgct gacgacgttg tcgaccgata tcgccttctg
      901 gatcttcgtg tcgcattcga gaataggcta aacctttttc tccattcaat gtttgttcac
      961 ctactggtac atggacacca tccaaagtaa agtcaatttt attattcact ttgatgccac
     1021 cgaccgcatt gatcaaatct tccatgccat ccaaattgat cgttacataa tgatcaatag
     1081 gaatatcgag cagttgctca attgaatcca tggccatttc gacaccacca aatgcatagg
     1141 catgatttaa tttatctacc gttccataac ctacgatatt cgtataaata tctcggtcca
     1201 aactgatgat cgttgatttt ttttgtttcg gattaactgt tactaccatc atcgtatcag
     1261 aacgtccttg ttccgttctt cctaatccac cggtatctaa acctaatagc aaaaccgaaa
     1321 aaggttcttt gtcatcaatg cttactctct cagaacgttt ggaggatatt cgattagagt
     1381 ttttagaaat tttgtttact gtttggctcg cttctcccat aaatctgatt ccgtatgctg
     1441 ataatccggc aatcagcaaa acaaataccc ctaacaaggt taatagaact ttttgccatc
     1501 gcttcataat acacctctgt ttcacatttt ctaaaatatt gtctcatata actgttcaat
     1561 cagcaaattc actttttaat cataagaaaa ctttaaaaaa catttttgtg taacgattac
     1621 tattttttaa aatttctcaa aattcttaac tatcatacca taccgtgaaa aagatcgaaa
     1681 gaccgaatac acgcagttcg caatcgtttt atcttttttt acgtattttt tccagaaaaa
     1741 gcgaacaaca aggtattttc caaataatcg tgcagtaaaa tgatcgcaca tacctttccc
     1801 atctttttaa cagtctttgc ctaccgttca gattatcatt cgatttcacg aaaatttctt
     1861 cctatgactg gtccttttgt taaacccatc ttctattcgt tttatttcca gctcactgca
     1921 tggctcttaa aatgactcga actagtgatt tcattaccat catcttttaa tggatagata
     1981 tgctcctctt caaagtaaaa cgattttctt ttaaagtaat agaattgaca tggtaatgag
     2041 tatgtatact agtggtttca caaatggtct tttattcaac atccaaaaac tgaaatttct
     2101 ttaaaatcca ttactaacga agaaaatgtt ccttgcttga taattagtta ggacatgtca
     2161 agtgaacaaa tgcatttact tagccaaaaa atctaaaaac ttcataaata gacaaaaaga
     2221 cacgactctc taagaagtcg tgtctttatt caacaaatta ttataattta gtcacttcag
     2281 cagcttgagg tccgcgtgca ccttcaacaa tcgtgaattc tacttcttga ccttcttcta
     2341 ggctcttgaa gccgtctcct tgaattgctg agaaatgaac aaatacgtca tctccgccgt
     2401 ctactgtaat aaacccaaat cctttttcat tgttgaacca tttaaccgtt ccgtgttcca
     2461 taaatgtata gcctccaaaa aagttcttgc aagtaacctt gcaagaacat tatacccaat
     2521 ttagaaaacg ctagcaagct ttcgctcttt tattttccaa attattttta agactgatat
     2581 gacggaggaa atggttattt tgctgcttcg aaacgtttgt ttacttcatc ccaattaata
     2641 acattccaga atgctgcaat ataatctggt cggacatttt tatatttcaa ataataagca
     2701 tgttcccaaa cgtccaaacc tagaattggt gtctttcctt ccatcaatgg agaatcttga
     2761 tttgcagtag aggtaatagc taattttcca ttttccatta caagccaagc ccatccagaa
     2821 ccaaatcgtc cagcggctgc ttttttgaat tcttctttaa aagaagaaaa atcaccaaaa
     2881 gcttcattaa tcgcttcttt tatttctcct gtaggttcgc caccagcatt tggtgccatg
     2941 atttcccaga aaaatgaatg gttagcatgt ccgccaccat tgttacgtac agctgtcttg
     3001 atatctgttg gaatagcgtc catatcagat aataattctt ctattgtttt ttcgcctaat
     3061 tctgggtatt tctcaattgc tgcatttaaa ttcgtcacat aagtattgtg atgcttatca
     3121 tgatgcagat gcatcgtttc ttcgtcaata taaggttcca atgcgtcata tgcataaggt
     3181 agatctggta atgtgtaagt cataaaaaaa ttcctcctca atgttttctt tataatgtct
     3241 acattacaaa gagtaacaaa gagaaggaaa ttcttcaatt gttatgccta gtcgtctttt
     3301 tcactttgac ctacttcttc ttcaaaaacc tcagtttctt taaaaataaa caacttgctg
     3361 aaaatatagt tcgctaggat aacaataatt tgagtgatga ttttcgccac atactcattg
     3421 ctgttcataa cattgatcaa caataccatt gcccccatgt ccaatccgta agaaaggata
     3481 cggtagaaga agaacttacc gcattcaata agtgcatgtg taaaattcgg tgattttgaa
     3541 tggaataccc aaagtttgtt cgttacaaaa gcaaacaata cagaacaaaa ccacgaaata
     3601 gaattggata aggccatact taaatcaaaa tattggcgtg cgagagcaaa actaacaaaa
     3661 tttacaatcg tagccaaacc accaaaaaag agatagacta gtatctccca atatcctttt
     3721 gcttctaaat atccttttaa ctgtcgatac tgcttcatga gccaacctac cttattaaaa
     3781 attcttattt atcatactct atatttcaat aactttcatc tttatttaaa aaaattaatt
     3841 ttttttgatc ctttgccttt gtctctgctt ggatcgtcac atgtgtaata ccatatttta
     3901 attttaataa agaaattata cgaggatata tcttttcaat ctcgcttagt agctgatctt
     3961 caaaatcaat atgtccgtcg aaaataatta tcttttcgct atactgccaa atatggacat
     4021 gatgaacatt ctttacccca tcgattgctt caatttcttg ttccattttt tcatagtcaa
     4081 tagcaggtgc actttgtatc aaaatagcgg cagctttttt gatcaccatt atcgcttcta
     4141 tcaatatata tatcgaaatg atcagtgtaa taactggatc gatccagtag atatcgaaaa
     4201 aacggatcaa aattgctccg ataatcacac ttaccgaaga cagtgtatca cttaacaaat
     4261 gcaaataact agattttata ttcaaacttt catctgcttc ttgtcgtaat aataatactg
     4321 agaacaaatt cgcgagcaat ccaataactg ctactgttag catcaaatga ctatttatct
     4381 tttctggtga agtgaaccgc ttgaatgctt caacggttaa aaacaaggat aatatgatca
     4441 atatgaatga gttgatgaaa gcactcaata tttctaaacg ctggtagcca tacgtacgtt
     4501 ttttatttgc ttcttttcgg gccatttttt gtgccccgta agaaaaaata attgccaatg
     4561 tatcactaag gttatggaaa gcatctgaaa caagagacaa actccctgac ataatccctc
     4621 caataaattc tgataaagtg atcaccgcat tcaaaaccac tactccaaat agtttttttg
     4681 atgatctctg tggctgtgta ggcactattc cttcctccat tttatttata ttggatagtc
     4741 tttagacaag aataacattt ttttgatatt tcaccaatca ttcagatgtt tccctaaagc
     4801 acttgtattt cacttgatac ttcgctagaa ttgcaagtga tgacactact agatgaggag
     4861 tacttatgac aacaaaacaa ttaattatca gttcctttat acgattcaaa gacctaaagg
     4921 agacgagaaa tgtcccattt tggaaagcca ttgtctatct tttagttttg agtataatta
     4981 tggctttgcc aatctctttt caagtttttc aagtcttaaa caacattcaa ttggatgggc
     5041 aaaagattgc tactcgcatt cctgatttta caatccaaaa cgggcagctt caaaccgaag
     5101 aaaaagaagg attcatttac caaacaaact ccattatctt cacatttgat ccagagggaa
     5161 aacgaacaga acaggacatc tctactgatt tgatggggaa ttttgttagt gtcgggatgt
     5221 taaaaaacaa acttatcata gctcttccta atacaggcac gacttctgct ttgttgaaca
     5281 ataaccaatt agaacttccc tacaccaatg aatctttaaa aaacttaaca ggtaaggagc
     5341 tccgttcttt tttaagtaaa gcatctatac cactttggat aaaagtcctg acatttatat
     5401 tttctattta tcctagtttt ctgaatctgc tgatcacttt gctatttacc aacattgctg
     5461 cttttctcta tgcacgcttt cgactaacaa aagctacttt tcttgattgt ttaaaaacat
     5521 tgatctattc aatttcattg cctactataa ttgcaacaat cttgatggtc tttcttcctt
     5581 cttttgacac aagcgccttc attgcttttg caggaatctt tatttttgct caagctgtca
     5641 aaggatgggc aaaaatatcg atcaattaag aaataaagac aactcgttat ttctacttca
     5701 atagaccctt ttagaggttg tgacattttt ttgtcacagc ccctttttta tttgaaaaat
     5761 atttctttct caaaaaacaa gcaaggcgct tcaaagttct tcttgtatgc gctacactta
     5821 gagtaaagaa aggcgggaaa ttaactatgt cagaaagaat catttatgca gatcgtccat
     5881 ttcgtgatga atttatcgaa gaaatccata ctatagcgcc tggttatatc tttaaaacag
     5941 aattacaacc agaggatcta caaaatgtag agatcagttt gggctggaac tcagactacc
     6001 aagaaaaact attagcctct tctaccttga aatgggtcca atctatctca gctggtgttg
     6061 atcagctacc ccttgagact ttttcaaaga aacatatttt actttcaaat ggtagtggca
     6121 tccacagtga atcgattacg gagcatgtct taggaatcat ccttgggtat tctcgtggtc
     6181 tttttcaagc acaaaaagca cagagtgaca tgaaatggtt aggttcatcg gtccattacc
     6241 aatctatcca aggaaaaaaa atactaatca ttggaactgg ccatattgga aaaatgattg
     6301 caaaaaaatg caaagcatta gacatgaact gctatggaat caatacatcc ggtcatccag
     6361 cagaaggaat ggaaaagact ttttctttaa atcaactaaa ggaagttctt cctgaagtag
     6421 atttcgtagt caatatctta cctctaacgg aggaaacaac tgggctcttt gatcagcata
     6481 tgtttgaaca ctttgatcca aaatctgtat tcatcaatgt tggccgtggt gcttctgtta
     6541 agacacaaga tctggtgcag gctttaaata accagcaact ttcctttgca gcattagacg
     6601 tttttgagga agagcctctt ccacaagatc atcctctttg gaaaatggac aatgtactga
     6661 ttacttccca cattgcaggt ttgacacctg attttcaaaa gaaattaatg gctatttttt
     6721 taaccaatct gaaaagctac tttgccaccc atgagcttca aaccaatcaa gtaaaattgt
     6781 ctgcaggata ttaatagctg aaaacaatat tttcaaaaga acaaagagaa ggtgacaaaa
     6841 ctggctctat aatttatggg actgatgaat cagaattgat tcttcagccc catttttatt
     6901 ttagg
//
LOCUS       DAZABD010000103         6829 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000103 DAZABD010000000
VERSION     DAZABD010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6829
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.107"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1382
                     /locus_tag="ACHALG_002646"
     CDS             <1..1382
                     /locus_tag="ACHALG_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFN2587967.1"
                     /translation="CGQFYVNKEVNLEKLSNLLHVCKTTLLNDINNIKKEFEEQIAYT
                     HREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDEPDYLQLTECEFISVSK
                     AYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRLGGFNSWELPESFFERA
                     DRFIESVTENSGRFYDKENKEILLIGFAISFLRQQVCAVTIDSKFIEEIKKRPIYNYV
                     ESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDFQQLHQVFIDNTPEIKE
                     LVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKFYLLNEEQTNLYKEVQT
                     IFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYIVTNSDVHYLIYREALE
                     AVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDAVQYENIIPISINTIDR
                     AIISAVQNK"
     gene            complement(1474..1839)
                     /locus_tag="ACHALG_002647"
     CDS             complement(1474..1839)
                     /locus_tag="ACHALG_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFN2587968.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(1836..2201)
                     /locus_tag="ACHALG_002648"
     CDS             complement(1836..2201)
                     /locus_tag="ACHALG_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFN2587969.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2393..2641)
                     /locus_tag="ACHALG_002649"
     CDS             complement(2393..2641)
                     /locus_tag="ACHALG_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587970.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2640..2768
                     /locus_tag="ACHALG_002650"
     CDS             2640..2768
                     /locus_tag="ACHALG_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HFN2587971.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2825..4519
                     /locus_tag="ACHALG_002651"
     CDS             2825..4519
                     /locus_tag="ACHALG_002651"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HFN2587972.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKMTNTLTQ
                     EEEHVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4619..4792
                     /locus_tag="ACHALG_002652"
     CDS             4619..4792
                     /locus_tag="ACHALG_002652"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587973.1"
                     /translation="MQAFEHRLPRGCEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQA
                     FEHRLPRGYEKVV"
     gene            5170..5970
                     /locus_tag="ACHALG_002653"
                     /pseudo
     CDS             5170..5970
                     /locus_tag="ACHALG_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
     gene            complement(6018..>6829)
                     /locus_tag="ACHALG_002654"
     CDS             complement(6018..>6829)
                     /locus_tag="ACHALG_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS256-like element ISEfm2 family transposase"
                     /protein_id="HFN2587974.1"
                     /translation="MYGYHYTPQTMSKMTKVLTEEVNAFKVRTLNDKYVAIFMDATYI
                     PLKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWNELLQDIYSRGVHDVLLF
                     ITDGLKGMKDTIHQIYPKAKYQHCCVHVSRNIAHKVRVKDRKEICDDFKAVYQASSKE
                     EANTFLGSMIEKWQKTYPKVTQSLIKNQDLLTFYEFPPGIRRSIYSTNLIESFNKQIK
                     KYSRRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTDTLASMFTE"
BASE COUNT         2201 a         1290 c         1252 g         2086 t
ORIGIN      
        1 tatgtgggca attctatgta aataaagaag tcaatctaga aaagctctcc aatctcctgc
       61 atgtttgcaa aaccactttg ctcaatgata tcaataacat aaaaaaagaa tttgaagaac
      121 agatcgctta tacacatcgg gaaaaggacc gttacacttt atatttttca gaacatatac
      181 ctcgatgcaa gatcatgcag caattgtctc aaaattcgtt gtttttaaaa acttgtctgc
      241 tttatttgga agaagatgaa cctgattatc ttcaattaac tgaatgtgaa ttcatctctg
      301 tcagcaaagc ttattcatta aaaaaacaag tattagctta ttttaatgac tgcgggatcg
      361 aaatcgaccg ctactcccct cgattcactg aaatggaaag acgtctgctg cttcttaatg
      421 tgtcgtatcg tttaggcggt tttaattctt gggagctacc tgaatcgttt tttgaacgtg
      481 ccgatcgttt cattgaatcc gtaacggaaa atagtggcag attttatgat aaagaaaata
      541 aagaaatctt attgattggt tttgctatca gtttcctacg ccagcaagtg tgtgctgtga
      601 caatagattc aaaatttatc gaagaaatca aaaaacgtcc tatttacaat tatgtcgaat
      661 cagcatggga aaacactgat ttccaaacct attataaaaa agaagaattc gcctttattt
      721 tgacactatt caatctttgt aattatggtt tccattctta tcagttgatt gcggaagact
      781 ttcaacagct acaccaagtg tttattgaca atactcccga aataaaagaa ctagttgcta
      841 cctttgaatc tcacttcaat caagaattat tcggaaacca accattcgaa cgtgcgctga
      901 ttcatctgat gcgttcggct tgggataact atcaattatt catgccagaa aaattttatc
      961 ttctaaatga ggaacagaca aatttatata aagaagtcca gacaattttt tccagctggt
     1021 ccagccagtt accttatgat ctacggttga atcctaattg catgcgggct tttgtgatcg
     1081 aattgagcgg aattctccgt ctgacaaaag aacatctgac tatctatatc gtcacaaatt
     1141 cggatgttca ttatctgatt tatcgggaag ccttggaagc tgtaactact ttcgattttc
     1201 aagttgcacc aacaatttat tcaagtatat ctgatataaa gaagtatgcg cagcagtcct
     1261 caaaccgggt attatgtgaa cgtacattat atacaccaga tgcggtccaa tatgaaaata
     1321 ttattcctat ttcgatcaat acgatcgatc gagctatcat ttcagctgtg caaaacaaat
     1381 aagggactgt gacaaaaatc atgtcacagc ctcttatttg ttgaagatgc ccatttctgt
     1441 aacaatctct tgatacacat caggctcaca acctcactta ttttggacat ctacgggtcc
     1501 gatctcttta tttaaaaaat aagaaatgat ggttcggata actaccaacg ccgctagttg
     1561 gaaaatatcc tgcaaggtag gtttgataat cgattcgatg atcccagctg aaacaagaat
     1621 ttccaaactt agaagtatat agcctcccaa catcttttta atagcattgt tctcgctgac
     1681 tcggtcgctt tgcacttttg tatggacaaa taaatatttc aaaaaatcct tgattgatag
     1741 acaaacaccc caaaccaata aacagatagc aaagatatca agtcccagta caagtttttc
     1801 aaaatatgga agaagaaact gcatcaactc tgtcgtcatc ctacctctcc tcttctttgt
     1861 ttttctttgt cttctatttc ctgattcaag aaataagaaa tgactgttcg aatgataata
     1921 ataagtgcaa gcttcaaaat atctgtccaa gtcggcttga taactgactc gatgatatca
     1981 gcaacgacta gtacttccaa gcttaacaaa atataacttg ctagcagttt tcttgtatct
     2041 gcattacgag tattacgttc tgcatagttt ttcttgacat gagaccaatc aaaaagtttg
     2101 aatagaacaa gaacgatccc gacaattaag ataccgatag aacaaatatt taaaatagca
     2161 atgatccatt ctagaaattc aattaaattt gagcgaaaca tatacgctac ctactttctt
     2221 ttaatttgtt cctattcttt tattgtaaaa gaaagttacc ttgttcgtct atttaaaagt
     2281 tgttctaatg ataacggttc gcttaaattt ttctttttca ctctcccctt tttctccctc
     2341 catccataaa tcgtggaagt atccaaaaca acgtagcaag ttaaaataag gcttagtccg
     2401 tcatcagctt ttcaaagaag cactgtctcg gtgctttttt tgtacccaaa tttctaaaaa
     2461 tagaaccata cacctcctca aatcttttct tcttacctac cctattttta tttacaggaa
     2521 aatcagacac ttcttctttt ttgtcaaaat ttcctttttc ttctcgtcct atcatttggc
     2581 attctaataa aaacgaacta tccttggata tagaaagaga aaacaaggag gcgtttttca
     2641 tgagtaaaaa attaatcaaa gtcggcatcg gtttaggttt gcttgccctt ggcgctgcat
     2701 atctaggaaa aaagacagga ctattcgaag atgacagtca tttatatgat gaattcgaat
     2761 caatctagca tcctatttag ttgaatcact acggacatac ttggaagaca ttggaggaat
     2821 cacaatgaga aaaagtaaag caataatgat tggtgccggt attgccaata tggcagcggc
     2881 tgtttatttg attcaagaag gcaaatggaa aggggatcag atcacctttt attcattgga
     2941 tgatcacggt tcaaatgatg gtgcaccgac cgacactgta gtggatgaat attggaataa
     3001 aaaccatcca ttagaaaacc aaaaaggcta tattgctcga ggcggtcgca tgcttaatta
     3061 ccgtacctat gtagatttga tggatctgtt aagtcgaata ccttctgtta ctgagccagg
     3121 gatgactgct gaagaagata cgagagattt tgatgcaaaa caccgaacct ttgacaaagc
     3181 acgtttgatg gaaggtggca aaggaatcat tgatggcgga aaactgggat tcaataataa
     3241 agaccgtatt cttctgacta aactgattgc tatgcctgat tcagaagaag aaaaactaga
     3301 caatatcact atcgaagaat atttcaaaga tgatccacac ttttttgaaa caaacttctg
     3361 gtttatgtgg gaaacaacat ttgctttcag aacacgtagt tctgctcaag aattgcgtcg
     3421 ctatatgcat caaatgattt atgaattcac tcaaatcgaa catctagtgg gcgtcaatcg
     3481 aacacgttac aatcaatacg aaagtattat gcttcctttg atcgagtact tgaaagaaca
     3541 aggatgcaaa atcattttga accgccgtgt cactgactgg gaattcaaag aaacaccgat
     3601 gcaagatgaa atcactgtga ctggtttgaa aatgaccaat acactgactc aagaagaaga
     3661 acacgttgtg gtcgatgaag atacagcagt tattttcact aatgggtcca ttacagattc
     3721 agctacttta ggagattacg aaacaccagc tgtagaaaat atggattatg gtgcagcttc
     3781 tagtctctgg aaaaaagcca gtgaacgttt ctacaacttg ggaaatcctg acaaattctt
     3841 tgctgatcga gatgcaagcg aatgggtcag ctttaccctg acgacgaaaa accatttgtt
     3901 gttgaatgaa atcacccgta tcacgactca agaaccagga aatgccttga attcatttat
     3961 ttcaaccaca ccgatcacgc cgttaggcca aaaagatgtc aatatgtcta tcgtcgtgca
     4021 tcatcaaccg cattttacgt ctcagaaacc aaatgaaaca gttatttggg gttatttcct
     4081 ttatccaaga cgacgtggcg aatttgtgga taaacagtat attaaaatga atggaaaaga
     4141 aatgctagaa gaactgattg gacaactttc aaaagtagat cctggaccag tgaatattcg
     4201 tgaaaaagaa acagaaattt tcgacagcgt aataaataat atccctgtct atatgcctta
     4261 tgcttccgcg ttgttcaata atcgtgccaa aagcgatcga ccaaaagtca ttcctaaaca
     4321 ttcgaccaat ctggccttta caggagaatt tgtcgagcag ccttatcaga tgatctttac
     4381 tgaacaaagt gctgtacgtt ctggagaaat cgctgctttc cattttgcgg gtatccctat
     4441 gtcacgtctt gtcaaaacac cgcgttacga caaagatatt cctactctag ctcgtgccgc
     4501 aaaaaaaatg tttgaataat caagaggttg tgaaaaagct gccctgcatc aagtaataag
     4561 aacaaccagg ctaaaacagc ttcttatgtt tttgatttgg ttggacttat taccgcagat
     4621 gcaagctttt gaacaccgtt taccaagagg ttgtgaaaaa gctgccctgc atcaagtaat
     4681 aagagcagcc aagctaaaac agcttcttat gtttttgact tggttggact tattaccgca
     4741 gatgcaagct tttgaacacc gtttaccaag aggttatgaa aaagtcgttt agtttcgagt
     4801 atgaagaaaa attttgtgaa aatggttcct cacgtttttg cataaatttg acttaatacc
     4861 gagaaactga tcccttctgt cataacctct ttttatttat gtctgtttct ttttgaaaac
     4921 aaccattttt tcaacgttta ctctcttgcc aatccaacat catctaacag atacaactat
     4981 ttcaatgatt accattacac taaaaaatcg gaaactcacc tctccgaaca caaatacata
     5041 agactataga atgtttcatt taagaaaaaa agcgtttcat tatcaaaaag gtaagatctc
     5101 ctttaaaatg aaattatctg tagtactaat aatgcagaca attttatgaa tctgaaagga
     5161 gcatctccaa tgaaagcagt cacttggcaa ggaaaacaga aaatggaaat cagaaacgta
     5221 gatgatccaa aaatattaga accaacagat gcaatcatcc gtatcacagc aacagccatt
     5281 tgcggctccg atctgcatct ttaccatcat ggtgaagcag ttttggaaaa agggtatgtt
     5341 gtaggacacg aaccaatggg aatcgttgaa gaagtgggat ctcaagttca aacattgaaa
     5401 aaagggcaac gtgtggttat cccttttaat attagttgtg gtcattgtca tttctgcctg
     5461 aatcacatgg aaagccaatg tgatgaatca aatccagatc agttatatgg cgggcttttc
     5521 ggatttggca agttgaacgg taatcattgg ggtggtcaag cggaatatct aagagttcct
     5581 tatgcagact ctacttcatt tgttgtacca gataatgatc ttcctgatga aaaagtttta
     5641 tttctatcag atattttgcc cacagcttat tggagcgttg aaaacgcagg agtaaaaaaa
     5701 ggcgatacag ttgtgattct aggttctggt ccagtcggtc tattcgctca aaaattcgct
     5761 gttatggctg gagcagagcg cgtaatcgca gtagatccag tacagcaccg tctagataaa
     5821 gcggctaatt acaatggggt tgaaacttat ttcttagaag acacggcaca agctggaaca
     5881 gaattgtatg aactggctaa aggtggcgca gatgtcatta tcgattgtgt aggaatggac
     5941 ggattagagc ctgtcaaaga aaaaggggag cgtcaataat tttgtgtaaa taactcgtcc
     6001 ttctgcaaaa taatgggtta ctcagtaaac attgaagcta atgtatccgt cacttgttgg
     6061 aagcctttat ggcttctatt taagaatttt tgattgtatg tatcaaagat ggatactagg
     6121 aaacgctcta gtgattcttc attttgaaac tgctcttttc tgcggctgta tttcttgatt
     6181 tgtttattga aagattcgat taaattagtt gagtaaatgc ttcggcggat accaggcgga
     6241 aactcataaa aagtcaataa atcttgattt tttatcagtg actgcgtcac tttaggataa
     6301 gttttctgcc acttctcaat catactccct aaaaaggtat ttgcctcttc ctttgagcta
     6361 gcttgataaa cagccttaaa atcatcacag atttcttttc gatctttgac acgtacttta
     6421 tgagcaatat tacgggaaac gtgaacacag caatgttggt atttagcttt aggataaatc
     6481 tggtgaatcg tatctttcat gccttttaag ccatcggtga taaacagtaa gacatcatga
     6541 acccctctgg agtaaatgtc ctgtaacaac tcattccaaa cgtaggtcga ttcagtcgga
     6601 gcaatcgcat aacttagtac ttctttagtg ccgtcttctc gtataccaat ggcaatatag
     6661 attgcttctt tggagacggt ttgccgtttt aaaggaatat aagtagcatc catgaaaata
     6721 gcgacatact tatcatttag agttctaact ttaaatgcat ttacttcttc agtcagcact
     6781 ttagtcattt tggacatggt ttggggcgtg tagtgatacc catacattt
//
LOCUS       DAZABD010000104         6826 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000104 DAZABD010000000
VERSION     DAZABD010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6826
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.108"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            28..195
                     /locus_tag="ACHALG_002655"
     CDS             28..195
                     /locus_tag="ACHALG_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HFN2587975.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(255..608)
                     /locus_tag="ACHALG_002656"
     CDS             complement(255..608)
                     /locus_tag="ACHALG_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN2587976.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            1113..1412
                     /locus_tag="ACHALG_002657"
                     /pseudo
     CDS             1113..1412
                     /locus_tag="ACHALG_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
     gene            1403..1744
                     /gene="mef(H)"
                     /locus_tag="ACHALG_002658"
                     /gene_synonym="mefH"
                     /pseudo
     CDS             1403..1744
                     /gene="mef(H)"
                     /locus_tag="ACHALG_002658"
                     /gene_synonym="mefH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_070509550.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide efflux MFS transporter Mef(H)"
     gene            1971..2390
                     /locus_tag="ACHALG_002659"
     CDS             1971..2390
                     /locus_tag="ACHALG_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFN2587977.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDRKISELYHVSRSAICSRRTRGLRKLKMLLSEGK"
     gene            2395..2634
                     /locus_tag="ACHALG_002660"
     CDS             2395..2634
                     /locus_tag="ACHALG_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN2587978.1"
                     /translation="MSKDYGYPSFELICKASSGDEMAVKEILKFYDGYISKLCLRPFY
                     HSESGKIIMQVDEELKGEIHTDMMKAILKFEIGVK"
     gene            3575..4615
                     /locus_tag="ACHALG_002661"
     CDS             3575..4615
                     /locus_tag="ACHALG_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFN2587979.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
     gene            5439..5771
                     /gene="traE"
                     /locus_tag="ACHALG_002662"
     CDS             5439..5771
                     /gene="traE"
                     /locus_tag="ACHALG_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFN2587980.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            6390..6731
                     /locus_tag="ACHALG_002663"
     CDS             6390..6731
                     /locus_tag="ACHALG_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFN2587981.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT         2397 a          928 c         1262 g         2239 t
ORIGIN      
        1 atagttatta taaactacaa gtggatattg tgtcctgtat gtggaaataa aacacgatta
       61 aagataaggg aagatactga attaaaaaaa ttccccctct attgtccgaa atgcagacaa
      121 gaaaatttaa ttgaaataaa gcagttcaaa gtaactgtga ttacagagcc agacgcaaag
      181 acgcagagcc gataaaatga gattaataca atctcatttt atcggctctt tccgttatgt
      241 atggattctt ttaattagtc ttcgatgttt cttgcttcgt tgataccgct ggctaaagat
      301 tccattaagg atagttcttt gtctgtaaag ctatccatgt atttctctat ctgtaatcgt
      361 cgggtgcttt ttaccaagtt attagcaggt aagaaaaatt catcaacgga aacatgaagt
      421 aacgatacaa ggtcataaag aacttgtatg ctggggtgtt gccctttatt ttcaatatta
      481 gttaagtacc gtgggtcaat ttcaatcaat gctcccactt gttcacgagt taaacctcgt
      541 ttcaatcgag cttctttaat ggctaaacca aaggctctaa aatcatattt atcttctttt
      601 ttacgcatag tagaccacct ctatacattt tattgttcct actgaattaa aaacaggtat
      661 agaaaaacgt gttatatggt ttataggttt atatttaata aaaagcacta ctaaacgcca
      721 ataaaaaaaa ccgttatatg gtagtgctat ttacgctgtt aaaatattgt atattacttc
      781 caaatggcgg tttgttggag gtcaacgtcg ccatgaagta catcatatac aataaatttc
      841 cttacattgg gttcttgtca aaaaaagtcg tctatctgca atagataagt acgtccacca
      901 atgtggtttt ataaatcata tagatagaat aacagaagca tgtaaacaga gaaataaatc
      961 tgtttatatg cttttttggc tattcagaac ttttttacaa agtttattta tcagtaatgc
     1021 aacaaatccc cctttcacat tgggactaag agtgaaagga gataaacgag caaggctcac
     1081 ttcctttcct agacagaaag ggggtgagaa acatgaaacc atcttctttt cagaccacaa
     1141 tagaaaatca gtttgactat atctgtaaac gtgctatgga agacgagcga aagaattata
     1201 tgctttatct ttcaaggatt gcaaagcgtg aggtgtcctt ttcggatgtt ggcgattatc
     1261 ttgttagcca gtttgcgaca acagataact attcaactga ctttcagatt tttacactca
     1321 atgggttatc agtaggcgtt gaaaatgatt tgttgagtga agcattacgt gagttgccag
     1381 acaagaaacg tgaaattcta ctgctgtttt acttatggga atttcgctat ttttagaatt
     1441 gcctgcaata gtttcctact gggtagttac agtgatgtgc tttgtggcta tgggtgtatc
     1501 tactatgttt gttgttcaaa tttacactct tgttcagaca caaacaccac cgcaattagt
     1561 aggaaagata atggctacgc tggtagcagt tgctatgtgc ggtcaaccta ttggacaaat
     1621 ggtttacggt attttattcg atgtatttat acaagaaaca tgggttgtta tgtttgtagc
     1681 ttctattgct tcaatcatga tttcaatata ttcaaaaaga atttttcaaa agttagagat
     1741 ataatacaag gggttaaata gtatgttagg ctaaaaacaa tatataaaag aaaatttgaa
     1801 atctgctaaa aggatagtgt tataaaagaa agagtaatat tgcagtattt aaggctcgca
     1861 caaatatatg ttttgtgtgg gcttttttct taccattttt ctgtatttct aaagggtata
     1921 aacggtattc cgtttatata tttcagcatt agcagaaagg gggtgagatt atgaggacat
     1981 cttctttcga gcatatcgtc agattacagt tcaattcttt aatgctgatt gtcattaaga
     2041 acaaagtgaa aagtcgaaac agacagcttg caagacattc taaacgtgaa gtattgtttt
     2101 gtgaaatagc cgaaacgaaa caagttgaac gtggaacgat tgatacctgt ttttatgatt
     2161 gtgtatcatt taaagtcttg cactttacaa tttatgtatc tgatgaaaca cttggtacag
     2221 ttttacatag actgtcagaa aaacagcgta gtgcgatttt attacgttat tttcaaggaa
     2281 tgaacgacag aaaaatttca gaactgtacc atgtatcacg ttctgctatt tgcagtagaa
     2341 gaactagagg actaaggaag ttaaaaatgc ttttgagcga agggaagtaa agaaatgagt
     2401 aaagattatg gctatccctc ttttgaattg atttgcaaag cgtcaagcgg agatgaaatg
     2461 gcagttaaag agattttgaa gttttatgac ggttatattt ctaaattatg cttgcgtcct
     2521 ttttaccact ctgaaagtgg taaaatcatt atgcaggttg atgaagaact aaaaggagaa
     2581 attcatacag acatgatgaa agcaatattg aaatttgaaa tcggagtgaa gtaatcaact
     2641 gtataaaaaa atcgggagat acacagtttc gtgctatctc ccttagtagt tttacggtca
     2701 tatcaaggct cattcaatcg tggtaaattc gtggtaagat gagtgttttt ctatacttca
     2761 aaatgcttga atgtatagtg tttatgcgga taattgaaaa ttagatataa aatttaagat
     2821 taattttttt atgacttgta caaatttatc tgatattctc ttattaagaa ctacctaatt
     2881 tgggtagttc ttatttttta cattttaagt agtatttaat tgcaatttgg tagtattttt
     2941 ttattatctt attcttagaa cggtagtact taatataaat ataggtagta tctaatttaa
     3001 gtattaatgg tagtatctaa attaacaggt agtatgtaac tactttaaag taattttttt
     3061 cagattaaca cagtagtatc ttattaaata agttaatttt aatggtagta cataattatt
     3121 atatatatct gacattggta gtacttattg tattttttag tagtatataa tctattgttt
     3181 tctagcctaa agccttgtct tataagaata atataatata tactactgaa ataaggtagt
     3241 atatattact agtaatggta gtatgtttta tatgcataag gtagttctta atttattttt
     3301 tgttttgaca ctggtagtac ttaattatct ttaattttaa aactaaattt tattcatacg
     3361 tttttctggt agtatttaat tataaataat atttatttaa agtagttctt aatgacttta
     3421 aaggtagtac ataattcatt ttatttctga ttttaggtag tatttaatta aaaaacagtt
     3481 ttaatgcgtt tgcatttttt atttatcttt gctattttcg aaaagagtag gtaattttac
     3541 ataaataaaa caacgtgaaa gtagaggatc tacaatgact gattttaaat tttttaaggc
     3601 ggatagagtt tacaacgaat tattttatca atttccaaaa gtctttattg tttctgacga
     3661 atacaaaaaa atgaaagatt caactaagat tgcctatatg cttttaaaag caagattaga
     3721 gatcgcaatc agcaaacggc aaatcgatga agaaggtaat gtttatttta cttatacgac
     3781 aaatgaacta tgtagagtat taaactgcca aaaacaaaaa gcgatagcaa tcaaaaaaga
     3841 gttggaatcc tttggtttat tattacaaaa gcagatggga tttaacaaac agttagggaa
     3901 aaataatcct aatagactat atctagcaga attaaaagtc tcagaaaatg atatctactt
     3961 actcgaaaaa tttgatagag agaataggga aaacgttgat aaatcagagg gtatgaaaat
     4021 catacccacc ctcgacgaaa aatcagacgc tgaatccctt ggggctcaag agggtatgaa
     4081 aatcataccg tgccaaaacg ttgataaatc agagggtatg aaaatcatac cagaacttaa
     4141 taataatata ttagacacta atagacacaa tatagacact gaaaaagacc gcctacaaga
     4201 tcaattgttg ttagacaatt ttgagacaat tatgacaaac gacagcattg ctacgtttgt
     4261 ccctgaacga gtattaaatt tgataaaaac attttcttca agttacagtg aagctcaaaa
     4321 aaccgtccag actattcata atgcaaagaa aaaagctgaa atagaaagtg atatttcgat
     4381 agtttttgaa gaactcgata gttattatgt caatgcagaa caagaattat acacgacact
     4441 gttaaaagcc tatcaaaaat taaaaaccga aaaagtcgaa aatatccaga acctgatttt
     4501 tgtctatgta aaaaattggt ttatcgaaaa accaatagct gctaaagtat caagtgaaaa
     4561 acgtttgaat tatgcaagct ccccaagcac tattacgaaa gactggttag agtgaaactt
     4621 tactgacaaa gtgattgttg cttcatggag taacattaaa attaaaaatt ttttattatt
     4681 ctaattatat tacgaatatt acaaaataat agctatttta atagaattta taagttaaaa
     4741 tattatgata aaaatcacaa acgtagcctt ttacgtcata agtgttaaaa gaaataatta
     4801 atttcaaata tagaaaaggg aacgtatgtt tgcatactcc ttttttttga gttataataa
     4861 tacgcgaaga gagataattt cattatttaa acaagctaaa aatcttgata ttacttcttt
     4921 tcaggcaata cgaatattat tcgcttacat tttttgtata aatttaattg tctgctatgc
     4981 ttgtgatgaa ttgattttcg ttttgcactt ttggatgata aatttggaca attgaaaata
     5041 caaaaaaaag aaaggcggta tgaactcgga aggctggtaa ccttcgtcag cgttcataca
     5101 cggcattaag atttggccct taatgcgtta acctttcagt gttacggtat tacccgcaat
     5161 ggatttagta taacagatat tcaaaagtga taccactttt tttggctgtg ttaaggttaa
     5221 aaatacgtga tattacgatg aatctatgcc taagcataat gttataaaaa tgtccgtgtg
     5281 gctctttgcc gtatgacgaa cgctgaaatt agagggaacg catgtttgga tgcgttccct
     5341 cttttttgtt tgtcatgaac gaaaatcaat tctctatttt tgaaaaataa caagaaaaac
     5401 ttgttaaaaa tatttaaaga aattgggtgg tttattttat gtcatataca ggaattttga
     5461 gtctcaaaga tatttgtcac tacggtaaac ggtgtaccgc tacagagaaa atcacgaaga
     5521 aactatcaac tggccaaagt aaaaccgttg tgcagtgtaa gaaatatata atccagaaag
     5581 acaaagtctc agaggaaatg atttattaca ttgggaaaca aaagcaaata attttaaaag
     5641 atcctatccc cttaaaagag ctatatccaa caatcaaaca tgtttatgac caaaacggtg
     5701 tactgattgg tcgaagaaaa aatggagtct tgcgttgtac cgcaaaaggg atggggcgtt
     5761 tgatcggctg aattgatcat tctcataact tgtattttat cgtttttcgc ttgcttgaaa
     5821 atattgtcga gacttctaaa ggaacttaga gtcactagaa aaataaggag tagacttgat
     5881 ctatcaacaa ttcccttcgg ggagtgacgg ggtaggtttt aacgattggt aacgtaaaaa
     5941 attggtcaat cgagtaaatg cgcttgtttg gatagtggtg aaatgaattt gtgtaaataa
     6001 taagcgcaga gtaaacaaga gggaatttag aagttagaca agttagaata gcgtctagat
     6061 taatcatgag cagctactaa gggatctaga ctttgacgca aactcatgga ataaggagta
     6121 ggtcttctcg taatggggag gacgcacaag aaattgtgtc gtgtgctgca gtacatgtag
     6181 atacttgcga ggacgttgat tattggatca atcgagcatt taccgctgtc tactagggtt
     6241 tctcgtttcg gacgccataa acgagtcgaa attttttatg tatttttaag taaacgaaaa
     6301 acgataaaat atgaacaagt cctgaataaa gtgaaaaaat taatataaag catagagaaa
     6361 atgaaattta cgtaatggag gaatgacaaa tgaaaaatta tgatccgaat atacgattgg
     6421 gcacacatac tattaaggtt tctttccaac gttgggatta caaaggattt gtgacgttta
     6481 gaaggggcgg aaattgtaaa ggattagatg ttttagcatt agacgaggat gatttatacg
     6541 atcaaacatt gactgataat ccaattggat tcggcttgct acctgaagat gatgaaggta
     6601 atgaatggtt taaaatgacg cttatgaatg ataatggaga cgaattatct gttgaagata
     6661 cttggtcata tttgagtgat tatattgtca gctttgaaat cattgaattt gtagcggata
     6721 aagaagaata aaaactggag gaataaattt tacagataaa ggcttagaag gagtcacgac
     6781 gattaataaa aaggtagtat taccttaggc attgagtaag gaattt
//
LOCUS       DAZABD010000105         6643 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000105 DAZABD010000000
VERSION     DAZABD010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.109"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..859)
                     /locus_tag="ACHALG_002664"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="ACHALG_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa10 family transposase"
     gene            1102..1557
                     /gene="tnpA"
                     /locus_tag="ACHALG_002665"
     CDS             1102..1557
                     /gene="tnpA"
                     /locus_tag="ACHALG_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFN2587982.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1822..1920
                     /locus_tag="ACHALG_002666"
                     /pseudo
     CDS             1822..1920
                     /locus_tag="ACHALG_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2143..2373)
                     /locus_tag="ACHALG_002667"
     CDS             complement(2143..2373)
                     /locus_tag="ACHALG_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HFN2587983.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(2603..3421)
                     /locus_tag="ACHALG_002668"
     CDS             complement(2603..3421)
                     /locus_tag="ACHALG_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFN2587984.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3582..4271
                     /locus_tag="ACHALG_002669"
     CDS             3582..4271
                     /locus_tag="ACHALG_002669"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFN2587985.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4285..5787
                     /locus_tag="ACHALG_002670"
     CDS             4285..5787
                     /locus_tag="ACHALG_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFN2587986.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            5800..6276
                     /locus_tag="ACHALG_002671"
     CDS             5800..6276
                     /locus_tag="ACHALG_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFN2587987.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6373..6483)
                     /locus_tag="ACHALG_002672"
                     /pseudo
     CDS             complement(6373..6483)
                     /locus_tag="ACHALG_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2174 a         1032 c         1203 g         2234 t
ORIGIN      
        1 acttgtcctc gataagaatt gtagcgccgt gatttacgtg tgtatgtcgt tacttttatt
       61 cctagcttct tgatcaggcg ctgaactttc tttttattga tagagaaacc gcggtttctt
      121 agttcattgg tcatccgtaa acagccataa tccttgtgct tctcccgaac tttaagcatt
      181 tccgcttcaa tctcttgatc tggattttta cggtccaatc gtttttgcca gtacataaag
      241 attgccttag ggaactttaa agtttctaga agctcaacta atttaaacgg tcctcggagg
      301 cttgcgatga ttcgtgcgat tgcttcattc gtcgttgttg ggcttcctgt tttcgcagct
      361 tcctcaattc ttttaaaaag gcattttcaa tttgaaggga acggactcgt ttctccaact
      421 cctcgatgcg ttcacgttct ttagacgttg ctttagttgt agattttttc ttatttttgg
      481 gttcattttt cttagacaag ataggtggac atcctttcgt ttttgagagt ccatcgatcc
      541 caccctcacg gaattgacgc atccaactag cgatcaaatt agggttgttc atgttaagtg
      601 tgttggcaac ttcacgatag gacagctcac ttgttagata taactctatc gcatccagct
      661 taaattgaac agaatatttt ttcttttgtc gctttcttac tagaccctct tcaccaaatt
      721 cttgataggc attgatccag cgcttcactt gtgatggagc cttaatggaa tatttctttg
      781 ccagataatc acttccgcct ttaccatcca agtaatcatg aacgatcttt aatttaagtt
      841 caaaagtata cttagccata aaaaataccg acctccaaaa gttagatttt ttggtctaac
      901 ttttgggggt cggtacaaaa cttaaaagtt ttagaccctt ttattttctt aatgacttca
      961 gtcagttttg tgcgtaaagc gtgcgaaaca agaaaaccgc gcgtttctac gcgtggaaga
     1021 agtatcttta gcgataaaaa ctcctttcga tatactagtg agtggctacc aaccgctcat
     1081 cagatcgaaa ggagtttttt catgtcaaat gacgataaaa gtttagcata tactcgttgg
     1141 aattgtaagt atcatattat ttttacacca aaatatcaac gaaaagtaat ctatgggcaa
     1201 ttacgaaaag atcttggaaa aatacttagg aagttgtgcg aaatgaaaga tgtggaaatc
     1261 atagaagcac atgcaatgct agatcatatt cacatgcttg ttcgagtacc accaaaaata
     1321 tgtatatcca gttttatggg gtatctgaaa ggtcgtagtg cggtactaat ccacgagcag
     1381 cacgcaaatc tcaaatataa atacggaaac aaatcctttt ggtcgaagag atactatgtg
     1441 agtacagtcg gattgaatca aaagacaaat gaaaaataca tccgtgaaca agaagctgat
     1501 gatcgcattc gatatattat aagtaaaaga gagtacgtag aaccatttaa aaaataaaga
     1561 gaagtaagtg agtgaagatg agcagttggc ggtcttttaa aaatcccctt ttaaggggca
     1621 gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt
     1681 tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat
     1741 tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat
     1801 tcaaacatta gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga
     1861 acacctttgg aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga
     1921 caattaagag tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat
     1981 gaactaataa ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc
     2041 ataaactttt aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca
     2101 taacattgaa gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt
     2161 cttataaaca agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt
     2221 tatagaaaac attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc
     2281 agtaggttcc tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt
     2341 atttaagaga ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta
     2401 cacgtttttc tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc
     2461 aagaaccgta ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa
     2521 taagctatca agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta
     2581 ggtttagcta agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt
     2641 tttctcttaa ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat
     2701 cacaaatata aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa
     2761 tggctgtttg taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta
     2821 tagcaataat ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg
     2881 ttttcccaga taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt
     2941 gggcttgaaa atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat
     3001 tttctaaatc aagactagta tttccactag atcccactcc aaatatatat atgttttttg
     3061 attgagtgat cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg
     3121 aatagagagc ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat
     3181 gttctttttt ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag
     3241 aaaaaccaat tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct
     3301 taatatcact catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga
     3361 cttttttttc agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca
     3421 tacaaactcc tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact
     3481 cctaaatact ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag
     3541 ttattatcca aaaataataa aaaattggag cgtttaggat aatggataaa aagaggtttt
     3601 tagatcttat ttcaaaaggt ttgattgttt catgtcaagc attacctggt gaaccatttt
     3661 acaaagagga agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag
     3721 ttggtatacg tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac
     3781 caatgattgg aattattaaa agagattatc caccagaatc tccattcatt actgccacta
     3841 tgagagaagt agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc
     3901 ttagaaatcg acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat
     3961 atcctcatca gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata
     4021 aagcaggaat agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac
     4081 aagatgggcc agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg
     4141 ctgagggaag gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag
     4201 gtattgttgt gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag
     4261 cattaagtta gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc
     4321 atgttaccaa ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta
     4381 acaaatgaga gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt
     4441 ttaagtatta tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca
     4501 attggtattg ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga
     4561 gtagcttatc tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa
     4621 gatgcaacac tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt
     4681 ttacacaatc gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca
     4741 agatttattc cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta
     4801 atttggccac caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt
     4861 agtttaggaa cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac
     4921 cacaccattt accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga
     4981 tcaacaattg ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact
     5041 ggtctgttta cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga
     5101 ttaccagcag catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat
     5161 ggtgggcttt attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt
     5221 gaatatatgt ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga
     5281 ttatcttttt attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt
     5341 ttgatcgact ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa
     5401 ttaattcctt ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc
     5461 aaaaaattta aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa
     5521 gtaataaaca aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt
     5581 ggaaaagaaa atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg
     5641 aatggggaga ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa
     5701 gtgaagggtg gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc
     5761 aatcaaatat taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag
     5821 aaaaaacaag aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta
     5881 aatgatcctg tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt
     5941 tcaaaaattt atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct
     6001 attaatataa aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt
     6061 gaattacaag gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa
     6121 acctcgttgg ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt
     6181 atagttgttt ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt
     6241 gctcatggga attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa
     6301 aataagctaa ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt
     6361 ctgttcattt ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt
     6421 aaagttataa aagcttcgaa atccattggc atttcgttgg aataccttga tatgattatt
     6481 caaatataaa atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg
     6541 tttactcaac ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga
     6601 accttagcac tatataccaa atatagtaaa atgctgaaag atg
//
LOCUS       DAZABD010000106         6568 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000106 DAZABD010000000
VERSION     DAZABD010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6568
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.110"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            91..972
                     /locus_tag="ACHALG_002673"
                     /pseudo
     CDS             91..972
                     /locus_tag="ACHALG_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            985..2595
                     /locus_tag="ACHALG_002674"
     CDS             985..2595
                     /locus_tag="ACHALG_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN2587988.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRMGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(2672..3331)
                     /locus_tag="ACHALG_002675"
     CDS             complement(2672..3331)
                     /locus_tag="ACHALG_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFN2587989.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(3614..4279)
                     /locus_tag="ACHALG_002676"
     CDS             complement(3614..4279)
                     /locus_tag="ACHALG_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HFN2587990.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(4426..5031)
                     /gene="dhaL"
                     /locus_tag="ACHALG_002677"
     CDS             complement(4426..5031)
                     /gene="dhaL"
                     /locus_tag="ACHALG_002677"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFN2587991.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(5059..6069)
                     /gene="dhaK"
                     /locus_tag="ACHALG_002678"
     CDS             complement(5059..6069)
                     /gene="dhaK"
                     /locus_tag="ACHALG_002678"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFN2587992.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(6044..6439)
                     /gene="dhaM"
                     /locus_tag="ACHALG_002679"
     CDS             complement(6044..6439)
                     /gene="dhaM"
                     /locus_tag="ACHALG_002679"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFN2587993.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
BASE COUNT         1724 a         1391 c         1233 g         2220 t
ORIGIN      
        1 aaaaaagttt tataaaaagg tataaaacct ttttattaaa ctatttaatc ttgtttagta
       61 tatattttta gaaaaaggag tggggttttt atggcagaaa tcgtcaaagt cacacatttg
      121 aaaaagagct ttggtcgttt cgaagcttta aaagatgtct ctttcaccgt acattcaggt
      181 gaagtcgttg gatttatcgg accaaatggt tctggaaaat ccacgacgat tcggacactt
      241 ttagggatta taaaaaaaga cggcggaacg gcaacgattt ttgggcaaga tgtatgggaa
      301 gatagtttat ctatccatga acgtctttct tatgttccag gcgatatctc tttgtaggga
      361 aatttgactg gaggagcgat catcgatctc ttcatgaagc ttcatggaaa agggagtgct
      421 caaaaaaaag atgaactgat tcaacgtttc tctcttgatc ctaagaaaaa agcaaaaaac
      481 tattcaaaag gaaatcgcca aaaagtgggc ttgattgctg ctttggctgt tgattcggat
      541 ctctatttat ttgatgagcc tacttctggt ttagatcctt tgatggaaac gattttccaa
      601 gaagaagttg aaaaattgaa agaacagggc aaatccatcc tactttcttc tcatattctc
      661 agtgaagtcg agcgactagc ggataaagtc gtgatcatac gcgaaggaac agtcgtagaa
      721 gaaggaacac tcgatgaatt gcgtcatttg actcgttcga ccatcactat agaaacagaa
      781 gcagagaata gtcacttagc acaattgaac ggtgtccatg actttgtgca aaaaggtcac
      841 ctcgctcaat ttgctgctga taacgaagct ttcgatgtga ttttagctga agcgtccaaa
      901 ttaggtgtga aaaaatttga atctattcca ccgactcttg aggatttatt catgagacat
      961 tatgaaggat gaggaggaag cgaaatgact gataaatttt cacgctggtc cacccttttt
     1021 tctcaaaacc ttagccgtga ctggaaaaag atcctgatat ggctagtcgg tgtcactgct
     1081 ttttctggtg gttttgtccc tgcgtttgaa gaattgactt ccggacaagg attagctgga
     1141 ttatacgaaa ccttgcaaaa cccggcaatg attgcaatgg tcggaccaac tccagcttcg
     1201 aatgctgctg agtatacttt aggagcgatg tatgcaaatg aaatgttgct tttttgcagt
     1261 ttgtttgctg ctgtgatctc tgttttacac gtggtgagcc aaacgagaaa aaaagaagaa
     1321 cttggtctga ttgaacttat tcgttccttt cgagtcggac gtcaagcaaa ctctttagct
     1381 gtcatcgctg aaagtattct gatcaatttg cttttagcaa tatcgatcag ctgtacgatg
     1441 atttttttcc aagcagattc gatcactgca gaaggtgctt ggctttttgg gctatctgta
     1501 ggtagtatgg ggattttggg gagtgccgtt gcactgtttg ctgcacaact gatgccaagt
     1561 tcagccagtt cgaatggtct gtctttagcg tttttaggtg gactttacct tctacgagca
     1621 ataacagatg tagctgattc ctccctttcg atgtggaatc ctttaggctg gtcttatttg
     1681 acctatccat ttacaactaa caactggatt cctttgcttt atttgttttt gttcagtcta
     1741 atcttgataa tcagttcatt tgttttagag gaacatcgag atttgaacac cggctatatc
     1801 cccgaacgtg aaggaaaacc gtatgctaaa cgttctcttt tatccttgcg tggtttatta
     1861 ttctatctaa ataaagggat gacaatcagt tggcttatca cttttggttt gatgggggcg
     1921 gcatacggct ctatctacgg gaatatgcaa cagtttttag aaagtaacga cttaatgaaa
     1981 cagatgttta tccaatctgg cgtctcaatt gaaaaaaact ttacaagcac gatcatggtc
     2041 gtcttgattt gtctagctgc tattttacca attgctagca tcaatcgttt atttacagaa
     2101 gaaacccacg gacgtatgaa tcagctgttc gcaacaaaaa tttctaggaa ccaactttat
     2161 tggaccaata tgttactagc tattctattt gctatattgg caatcctgat tagttctggt
     2221 gcactagggc tttctgcttt ggcttcgatg aaggatgcga aagacatgga tctattgatg
     2281 tttcttaaag ccgggttcaa tttcctgcct gccgttttag tgtttatcgg aattagtggc
     2341 tttctattcg gatggcttcc tcgaatgggt aaacttgctt atatctactt agcttactcc
     2401 tttgcactca attactttgg cggaattttg gatttaccag aatggttttc caaaacagct
     2461 gtccctagct gggttccacg gatgccaaac gattcttttg atctttccgt gttccttacg
     2521 ttatcatgta tcagccttgc actgttcgtc cttggttcta ttggttatag gcgaagagac
     2581 ttactagaag gataataaaa aagcatgtac aatttcatgc tagcctccaa atctgggtca
     2641 aaaatccaac atttggaggt cattttttta tttacaagtc ttctaactgc tctttatcaa
     2701 tgatccagac ttctcgattc tttcttttta cataaccatt ctcctctaat aatttaaatt
     2761 ttctagataa agtttcgggg gtcgtaccga taaatgcagc aagttctttc attttcattg
     2821 gcaggaaaaa gtgatccttc cctgtcgctt tagctaagtc aagcagatag acagcgactc
     2881 gttcttctac tttttccatc atcaaaaaag ttgtctgctg ctgtactttt gttaatttct
     2941 caacgttgat tgctaaaagt ttcagactaa gggcgggata atgcagtaag agttccgaga
     3001 aatctttttg tctcaatatg caaatttccg ttttttctag tgcttcgcca aataaagact
     3061 catttggaac gcctagcaac tggctctctc cctcatatcc gcccggctct attactcgta
     3121 aaagctgttc ctttccgcta gcagataatt gatacacttt catactgccg gaggccacga
     3181 tcactaattg aggttctcca ttcggcgcaa gaatctgttc tcctttttcc acatagtgat
     3241 gtttgacaag tgcgtgtact ttttcttgat cttgctgtac taagtgattg aacaaaggaa
     3301 caaatgaaac acataaatga tcatgactca taatctctcc tccttctaaa cagctatttc
     3361 taataaagaa acataacttc ccttattcca actgataatt ttcttttatc atagcataaa
     3421 cgtcaagcga accagatacc agcaaataga gtggatttct ttttgtccct tgctttattt
     3481 tccttctttt gagaccttaa gtaattgatt cctatcaatt ttttaaatat ttactttttg
     3541 aataatcaaa aacaaacctt cactcgcaag ccgagagtaa ggtttgtttt tcgcattgct
     3601 tatttcgtgt tgtctattga aatttttccg cacctagctt ttgatatagt ggtgctagtt
     3661 cttgtaaggt ttcagcggtt atttgctcaa cgtgttcgat ttcttgcgga tagaagaata
     3721 gtttacccat ttgaaaggtt cggtctctca tcgctttttt caactcttct tgtttctttg
     3781 tatcggacaa atagtcagac aaagctaagt ggtcgtcaag ttcatgttct ggttgcggcc
     3841 atatgtaata atccttgata tcgactgttt tgctccactc tttcagtatc gcaagcaact
     3901 ggttatacgc tgcaatgcta ccatatcgtt cttcgctttt ataatgttgg tacatcaaat
     3961 agatttggta ttgtttttga ttcaacaaaa ctgctagaca ggcattctcg tcttgacgag
     4021 attcgcttcg gtacgcgctc caaaaatgac tgcgtaaatt ccagccattc gtccagcttt
     4081 cgattttagg tttgctcatg ccgtacatat caggcaaaag tgcagctgtc tggagctgaa
     4141 tctctttcca tttttgccag tgttctttat attctttctt gatttgttca atcttatctg
     4201 ttggatggct ctctttgagc tgtttgaatg cgtagattgg tcgatcaaac aactgataat
     4261 ctgattttcc aagttccatt gtttcgcctc tttccgttat cttttgtatt ctacctgaat
     4321 tttgcgatat ggacaaatag aggttgtgaa aaagctgttc tgcatcaagt cataagaaca
     4381 gccaaacaaa aaactgcgat gagcatcacc gcagttttat tgaacttaag gttgattgat
     4441 cacatctaaa agcgttgaaa ataaaatggc actagaagca gctcctgggt cgatatgtcc
     4501 gattgcacgc tctcctagat aggaagctcg tcctttttta gctaccaaga ctttggtgtg
     4561 ttcttttgcc tggtcaatga tttcttttgt taaattccct gccttcaatg cctctgtgac
     4621 tggcagccaa acatctaaca tcgttttatc gtcagcttct gcctttcctc ggctttgaat
     4681 acccgctgtt ccctgctcga tcacttcgcc taatttttct tgagaatcaa tagaatcgac
     4741 ctcttttgtc gctttcatca tgttcatgaa tgcagatccg tataacggac cagaagcccc
     4801 accgactttc gatatcatcg ccatagagaa tactttgaat gtatcgctga ttgtttcagg
     4861 atgatcggct tgaaaagctt cttcataagc tgaaacccct cttcccatgt tatttccatg
     4921 gtctccatcg ccaatcgggg tatctagatc actgagatat tgtttgttct gattgattgc
     4981 ctctgcaaat tgcgtgagcc attcttggat ttcttgaacg gttagtttca tattatcacc
     5041 cttttatctt gagatttttt accatgaaat cgtctgtaca ttgctttcta aagaatcttt
     5101 ccattgtgtc tccgtcaagt cgatcagtgt tagagaaaga ccttgcatat ctagtgaggt
     5161 cataaaatta ccgacttttc ggaaaactac attgacgcct ctttcttcca acagagctaa
     5221 aacatcattc atgaacacga actgttccat caaaggcgtc ccgcccattc cgttgactag
     5281 tacacctgct tcttttggta aattcgagta atcatctaaa atcttagtaa caagttcctt
     5341 tgccagtact ttagaaggct gcatcttttc tcgacgatag cctggctcac cgtggatacc
     5401 aacaccatac tcgatctcat cttctcctag ttcgaatcct ggtttcccta cttctggtac
     5461 agttgctgct cgaagcgcta gaccgatcgt cttagttgcc tgtacgacct tttctcccag
     5521 ttctttcaac tcagctaggg aagcaccgtt tcttgctgca tcgcccacga ttttgtgtac
     5581 aaggacagta cctgcaactc cgcgttttcc agcagtatac gtactatttt caacggcgat
     5641 atcatcatct acgatcacac tttctacttc aatatcgtcc atagcagcta attcctttgc
     5701 catatcgaag ttcaatgcat cccctgtata attcttaacg atcagcaaaa ctccttttcc
     5761 actatccgct tccttaatag ccgtctgaat ctgatctgga gttggagaag taaacacatc
     5821 accaagcacc gctgcactta acatcccttc ccctacaaac ccggcatggg atggttcatg
     5881 tccgcttcct ccaccagaaa ccaaaccaac ttgttccgct ttctgattct tggctacaac
     5941 acgggaagat tctacacgat gaacaagttc tggatagctt tttactaatc cttccagcat
     6001 ttcttcgaca atattgcttg gatcattcat gatttttttc aatttattcc catcctttct
     6061 ttccggcatt ttgtgcttct gttaagattt gctgagtatc atctgttata ccggcagtga
     6121 tggctgctgc aaaagctcct tccactaaag gcgcatccac cagatgataa cgttgctgct
     6181 gctcttcttc caacatgtct tttgccagtt ccgagttcaa taccgcgcta ccgatatccg
     6241 caaaaacaaa ataactagct gcatcgcttg attcattgac tgcttctacg attttcatcg
     6301 ggtctgatcc gatctcgcca tttattccgc cgagagaaaa aatttgcacc tcttcggatt
     6361 tttgcatctg ctcaatcatt tctttaatac catctgttac tttttgacta tgagaaataa
     6421 gcaaaatact ttttttcaca agaatcttcc tttcttttac atagttgcta acgattggtt
     6481 tcgctttcat taatgaacaa ttcaagtata taataaattt ctaattttgg gaaaatatgt
     6541 gtagtgcttc ttgaagtttg ttatactt
//
LOCUS       DAZABD010000107         6403 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000107 DAZABD010000000
VERSION     DAZABD010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6403
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.112"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(197..370)
                     /locus_tag="ACHALG_002680"
     CDS             complement(197..370)
                     /locus_tag="ACHALG_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587994.1"
                     /translation="MSMLVRKLPLSVREFLFYLVCLIASGFKLIEHIFIFAYVLAMIA
                     FLSGGWLIIYLLS"
     gene            complement(373..771)
                     /locus_tag="ACHALG_002681"
     CDS             complement(373..771)
                     /locus_tag="ACHALG_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587995.1"
                     /translation="MRLRRNKNYTTIILEDFTDQELLIVGKTKADFIEKGNASIVKAS
                     KVAVEKYFDSLEESAKEIFNGEKAGSYELDGIKFSLIPTTENIIPKKLYYIFSSEKHT
                     SMKPCIFDWQWLIDAVLDVKCSKYNITLGC"
     gene            987..2042
                     /locus_tag="ACHALG_002682"
     CDS             987..2042
                     /locus_tag="ACHALG_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587996.1"
                     /translation="MKEPLLFSVDLGNKSVKEMSTHVSPLSLPSRILNKNIINSGRFS
                     NIGSSLKRSLNVSEYSLVNTTESYYFGKDIHELGKDDYVIESLGFGISRYKQETYQDI
                     LSFSLANLARLEDQSDDVLTVNLVLGLPSSDYTETIVKYVMQQATRQHSVEVDGKAYN
                     INVDTVWVLPQPIGTLYNLLLADDGSVQDESLISQKIGFVDIGGGTKLFDVVQNFEID
                     ENNREQKDTGIFTLYNRIVNMLDLPISPNAFQLEKIIREGKEKGVYLFKPSGNESFDI
                     SEVVEREIKTYTNMVVAEVLATFKNFTTLDKVVFTGGGASIIDKQLVKDKIPHTLFVE
                     DSEFSNVKGFYKFGLTQ"
     gene            2062..2415
                     /locus_tag="ACHALG_002683"
     CDS             2062..2415
                     /locus_tag="ACHALG_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587997.1"
                     /translation="MNKGKSERYVVIINREKVSELPLAEFLESEYETLGVKYSQTIKK
                     ALNLYRRVKNNEVTIIEAGNDRNDSKVAEVKNKPTDHNPIQQAHPSTVTSDHSAKKEK
                     PMLKGFNAPSNKDLL"
     gene            complement(2597..2884)
                     /locus_tag="ACHALG_002684"
     CDS             complement(2597..2884)
                     /locus_tag="ACHALG_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2587998.1"
                     /translation="MKENRFKKFAEAEVEEVPEVVEAVEEPKQTPKETTSSLVAKLKS
                     DSSEKKKNYQFTLQPSQRKKLSKISEKLGIRSDSALLGEMIDNLYDEVFGE"
     gene            complement(2897..3715)
                     /locus_tag="ACHALG_002685"
     CDS             complement(2897..3715)
                     /locus_tag="ACHALG_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFN2587999.1"
                     /translation="MEKKLSIITGNIEKGGTGKTTTIYNLGGYAVENLGMKVLYIDED
                     KSQNLSKRFPDAFSKAKRENTINHLYLHGSAEPLAINENMDILVAGEDLKQVEMQMRD
                     MPNNRTLLFRWIVVNYEQLKEKYDLILIDTHNDAGLLAQNAWAVSDLVVGISDPSKDG
                     FEALLKLGKDIDALAKQLIDIKTGETLMETKYATIGSRIKHNTNSSREFLELVPTLSN
                     YLGYVQEKELLNATSLEGKNIAEFAKDKNIYRKNKDFFESTFTLYNKIIDTAQE"
     gene            3941..4162
                     /gene="relB"
                     /locus_tag="ACHALG_002686"
     CDS             3941..4162
                     /gene="relB"
                     /locus_tag="ACHALG_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFN2588000.1"
                     /translation="MPVISIRVSKEEKDFLEKVAEFNGDNISEFMRSNSLQTAETLVD
                     FATYKQLMKEHEENDQSISHEDMLKELGL"
     gene            4164..4430
                     /locus_tag="ACHALG_002687"
     CDS             4164..4430
                     /locus_tag="ACHALG_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFN2588001.1"
                     /translation="MTYQVRYEREAQKALKKMDKFQAKVILNWIEKNLVGTDDPRKHG
                     KGLTANKSGYWRYRVGAYRIIADISEHEVTILILSIGHRSEVYK"
     gene            4597..5082
                     /locus_tag="ACHALG_002688"
     CDS             4597..5082
                     /locus_tag="ACHALG_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2588002.1"
                     /translation="MGWYGDYSSANEVIEEKKGYAKNGLEIVGTKVTKTYGIILFKDA
                     EGTYDIDSFIFRDEMYKPIYFLNDSPAYFKKIPKKWLKFFVGDQEKMVKLQLDRIKEE
                     SNKPKLDDLLVAGKTYSIWDGSYVAVYQYKLKRSFIFRLQDGSLTRFTRLSAGDVKEV
                     T"
     gene            5291..5473
                     /locus_tag="ACHALG_002689"
     CDS             5291..5473
                     /locus_tag="ACHALG_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFN2588003.1"
                     /translation="MINLFLLVTASTLFFTGISLIISYLIYKKAENHTSKAIGLAVSS
                     FLTLLTLFVTLSFSFV"
     gene            5576..5860
                     /locus_tag="ACHALG_002690"
     CDS             5576..5860
                     /locus_tag="ACHALG_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2588004.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGIVKQLIKDREFKKFNSRQAYN
                     RQLKKAILKHKDSFIGNLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            5876..6112
                     /locus_tag="ACHALG_002691"
     CDS             5876..6112
                     /locus_tag="ACHALG_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2588005.1"
                     /translation="MEGKTFAEIWTEFIEVPTRIDPITSEKVLDADFYNLKAGTSLEC
                     VWMYLEEHFKGVTLGHVLYHNGAEWDAIGNIHFS"
     gene            6134..6388
                     /locus_tag="ACHALG_002692"
     CDS             6134..6388
                     /locus_tag="ACHALG_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2588006.1"
                     /translation="MNYTDEEQIALEYVFTNEEGEEIEKVLIISSKDLEKALAYEPYE
                     STVTVIEFLDTYTSNDTWEIMNICNANNIQFAEKTNKSFY"
BASE COUNT         2206 a         1094 c         1023 g         2080 t
ORIGIN      
        1 aaaaaaagta tcaacataaa catatacata tacacgttta tgttgataca cttaatttgt
       61 cctttaaaaa gcacttattt taatattatg ttattttata atcttattac aagctgattg
      121 ttagacgttg tcctttcatt acatttatta acggttattt tatcgtttaa tttattaatt
      181 atttttgatc taaaacttag cttaacaaat aaataattaa ccaaccgcca gataaaaatg
      241 cgatcattgc taatacataa gcaaagataa aaatatgttc gatcagctta aaccctgatg
      301 cgattaagca gaccaagtaa aatagaaatt ctcttacaga taatggtaat tttctaacta
      361 gcatactcat aattaacacc ccaaagtaat attgtatttt gaacatttta catccaatac
      421 agcatcaatc aaccattgcc aatcaaaaat acaaggcttc attgaagtat gtttttcaga
      481 tgaaaagata taatataatt tcttcggaat aatattttca gttgttggga tcaagctaaa
      541 tttaatacca tctaactcat agctaccagc tttctctcca ttaaaaattt ccttagcact
      601 ttcttctaaa gaatcaaaat atttttcgac agcaaccttt gaagctttta ctattgaagc
      661 atttcccttt tcgataaaat ctgcttttgt tttaccgaca ataagtaatt cttgatcagt
      721 aaaatcttct aaaataatag tagtgtaatt cttatttctt ctcaatcgca tcaggatcac
      781 tccttaatat aatttatgct tttataatat cataataaga taaatttgca agtgtttatt
      841 tacacctttt tatttacact ttattgtaat tgattatact tttttgattg catttttttc
      901 ttataaacag ttgcaactat tagtaaatag gtgtaaacta ttttcgtacc gaaataccat
      961 aaacacaaac agaaagaggt cataccatga aagaaccatt attgttttcc gttgacttag
     1021 ggaataaatc agtaaaagaa atgagtacac acgtttctcc actctcgctt ccctctcgta
     1081 ttttgaacaa aaatatcatt aattcaggac gtttttctaa tataggttcc tcattaaaac
     1141 gatcactaaa cgtttctgaa tatagcctag taaacactac tgaaagctat tattttggga
     1201 aggatataca cgagctagga aaagatgact acgtgataga atcactaggt tttgggatca
     1261 gcagatacaa gcaggaaacc taccaagata tcctatcttt ctctttagct aacctagcac
     1321 gtttagaaga tcaatctgat gacgttttaa ccgttaatct agttttaggt ctgccttcat
     1381 ccgactatac cgaaacaatc gtcaaatacg tgatgcaaca agccacacga caacattcag
     1441 ttgaagttga cggtaaagct tataatataa acgtagatac cgtttgggta ctgccacagc
     1501 cgattggaac actatataac ctcctccttg ctgatgatgg tagtgtacaa gatgaatcat
     1561 tgatcagtca gaagatcggt tttgttgata ttggtggtgg cacgaaactt tttgatgttg
     1621 tccaaaattt tgaaattgac gaaaacaacc gtgaacaaaa ggataccggt atttttactt
     1681 tatataatcg tatcgtaaat atgcttgacc ttccgatcag tcctaatgct ttccaacttg
     1741 aaaagattat ccgagaaggc aaagaaaaag gcgtatatct atttaaacca agcggaaatg
     1801 aaagttttga tatttcagaa gtagttgaaa gagaaataaa aacatacacc aatatggttg
     1861 ttgcagaagt tttagctaca ttcaaaaact tcacaacctt agataaagtt gtctttactg
     1921 gtggtggggc ttctattatc gacaaacaac ttgtaaaaga taaaattccg cataccttgt
     1981 ttgttgaaga cagtgaattt tctaatgtaa aaggctttta caagtttggt ttaactcaat
     2041 aaaacagcga aggagatcaa catgaataag ggaaaatcag agcgttatgt agttataatc
     2101 aatagagaaa aggttagcga gttaccttta gcagagtttc ttgaatctga atatgaaaca
     2161 ctaggcgtta aatactctca gacgatcaaa aaagctttga atctatatcg gcgtgtaaag
     2221 aacaatgaag ttacaattat tgaagcaggc aacgatagaa acgatagcaa agtagctgaa
     2281 gtaaagaata aaccaactga tcataaccct attcaacaag cgcatccttc aacggttaca
     2341 tccgatcatt cagcaaagaa agaaaagcca atgctaaaag ggttcaatgc accaagtaac
     2401 aaagacttac tttaaaaata aaaaggcgag gttacaatac ctagcctttt tattatgtac
     2461 aaatacaaac ataaacatat acgtatacat gtatataaac atgtatacgt atatatccat
     2521 aaacacgtat acgaaattta gttaagctat acctaaccgc ttattccaat aaaaaaagtg
     2581 atcagcttta aactgatcac tcaccaaaaa cttcatcata caagttatct atcatttcac
     2641 caagtaaagc tgaatctgat cgaattccta acttttcgct gattttagat agtttctttc
     2701 tttgtgatgg ttgaagcgtg aattgataat ttttcttctt ttcagaacta tcacttttta
     2761 gtttagctac caaagaactt gttgtttctt taggtgtttg tttaggttct tcaactgctt
     2821 caaccacttc aggaacttct tctacttcag cttcagcgaa ttttttaaat ctattttctt
     2881 tcacttcaat cactccttat tcttgtgccg tatcaattat tttgttgtat agcgtaaacg
     2941 tgctttcaaa gaaatcctta ttcttacgat agatattctt atccttagcg aactcagcaa
     3001 tattcttacc ttctaagcta gttgcattaa gcaattcctt ctcttgtaca tatccaagat
     3061 aattagataa cgttggtacc aattctagga actcacgact tgaattagta ttatgtttga
     3121 tcctgctacc gatcgtagcg tatttagttt ccatcagtgt ttcaccagtc ttaatatcaa
     3181 tcaactgttt tgctagtgca tcaatatctt taccaagctt aagcaaagct tcaaaaccat
     3241 ctttagatgg atcagaaatg cctacgacaa gatcagatac agcccaagca ttttgagcta
     3301 gtagacctgc atcattatga gtatcaatca gaattagatc atacttctct ttaagctgtt
     3361 catagttaac aacgatccat ctaaacagta gggttctgtt gttaggcata tcacgcattt
     3421 gcatctcaac ttgttttaaa tcttcacctg ctactaaaat atccatgttc tcatttatag
     3481 ctaaaggttc agctgatcca tgaagatata aatgattgat tgtattctct cttttcgctt
     3541 tactgaaagc atcaggaaat cgttttgata ggttttgcga cttatcttca tcaatataca
     3601 gcaccttcat acctaaattt tcgacagcat atccacctag attgtagatt gttgttgttt
     3661 tacctgttcc acctttttca atgtttccag taatgattga caacttcttt tccatgatta
     3721 aacctccctt attaatcttt tgtaactcca ttatacacta taaacacgtt tatgtatata
     3781 tttatataca taaacgtgtt tatatttata tttgctttta tattctacaa cataaacata
     3841 tatatgtata tgtttatgtt tatgtatata ttcaaatata taactattgc atttgtcata
     3901 caatcgtagt acaatgtaat tgaaaaggag ttgataaact atgccagtaa taagtattcg
     3961 tgtatctaaa gaagaaaaag attttcttga aaaagttgct gaattcaacg gagataacat
     4021 ttctgaattt atgcgtagta attccctgca aacagcagaa acgttagtag actttgccac
     4081 ctacaagcaa ctaatgaaag agcatgagga aaacgatcaa agtatttccc acgaagatat
     4141 gctaaaagag ttaggcttat aagatgactt accaagtaag atatgaacgt gaagctcaaa
     4201 aagcgctaaa aaaaatggat aagtttcagg caaaggttat cctaaactgg atcgagaaaa
     4261 atcttgtagg cactgatgat ccaagaaagc atggtaaagg tctaacagct aataaatcag
     4321 gctactggcg atatagggta ggtgcatacc gtataatagc tgatattagc gagcatgaag
     4381 tgacaattct tatattgtct attggtcaca gaagtgaagt ttataagtaa cagacaagct
     4441 gataaggctt gtctgttttt ttgttaaccc aacaaaatca ctgtttacga tcccacttta
     4501 cctaaaaaag aacaatatcc tcttgatatt atacacacca gtgtgtataa tataagtgta
     4561 aacaagctaa ttaactttca aaaggagcga ttttttatgg gatggtatgg tgattattca
     4621 tcagcaaatg aagtgatcga agaaaagaaa gggtacgcca aaaatggttt ggaaatcgta
     4681 ggtaccaaag taacgaagac ctatggaatt atccttttca aagatgcaga aggaacctac
     4741 gatattgaca gcttcatctt tcgagatgaa atgtacaaac caatctactt cttaaatgac
     4801 agtcctgctt actttaagaa aattccgaaa aaatggttga aattctttgt cggtgatcaa
     4861 gaaaaaatgg ttaagctaca actagataga attaaagaag aaagcaataa accaaaactt
     4921 gatgacttat tagttgcagg taaaacttac tcaatctggg atggatcgta tgtcgctgtc
     4981 tatcagtata aactcaaacg tagctttatt tttcgactac aagatggatc actcactcgg
     5041 ttcactcgac tatcagcagg agatgttaag gaagtaacct aaaacttaaa tacataaaca
     5101 taaacgtgta tacgtttatg tttatgtata tgtatatgaa tataaacgta ttaggtacga
     5161 acacttttaa attcttttct gggctttgtt tttagggtag ggctaaaaaa aacatcacca
     5221 tctgatccaa aacagctcag aaacgcattt aaacgtatct gataccaata ttacagaagg
     5281 agtgtatcgt atgattaacc tatttctttt agtgacagct agtacattat tctttactgg
     5341 aatatcactg ataatttcct atttgatcta taagaaagcc gaaaatcaca caagcaaagc
     5401 aatcggacta gccgtttcat cctttttaac tttgctgact ttatttgtta ctttatcgtt
     5461 ttcttttgtt tagaacagct gatccattag cacctttttc tctttacatt atacacactg
     5521 ttgtgtataa tgtatttgta aacaagctaa ttaactttta ggaaggagtg acaagatgct
     5581 aacagctact ttacagcaac ctactcacgt tgctattgca gaaagttata caattaatgc
     5641 agggatcgtt aaacagctga tcaaggatcg agaattcaaa aagtttaaca gtcggcaagc
     5701 gtataacaga cagctaaaga aagctattct aaagcacaaa gattccttca tcgggaactt
     5761 ggaaagtgta gatgttaaaa ctgtttcaac ggcattttca gttgttgaag tgactattga
     5821 cggtcgcttg atttctctat ttgcttttgg aattaattga tagggagtgt gaactttgga
     5881 aggtaagact tttgcagaaa tttggactga atttattgag gtaccaactc gcattgatcc
     5941 gataacttct gaaaaggtat tggatgcaga cttctacaat ctcaaagcag gtactagcct
     6001 tgaatgcgtt tggatgtatt tagaagagca tttcaaaggc gtaacgctcg gtcacgtctt
     6061 ataccacaat ggtgccgagt gggatgcaat tggaaatatc catttttctt aatataagaa
     6121 aggagaacac aatatgaatt ataccgatga agaacaaatc gctttggaat acgttttcac
     6181 aaatgaagaa ggggaggaaa ttgaaaaagt cttgatcatt tcatcaaaag acttggagaa
     6241 ggctttggct tatgaaccat acgaatcaac cgtaactgtc attgaattct tagataccta
     6301 tacttctaat gatacttggg aaattatgaa tatttgcaac gctaataaca ttcaatttgc
     6361 tgaaaaaacc aataaatcat tttattagtt ttgcaataaa aca
//
LOCUS       DAZABD010000108         5912 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000108 DAZABD010000000
VERSION     DAZABD010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5912
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.113"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(99..1229)
                     /locus_tag="ACHALG_002693"
     CDS             complement(99..1229)
                     /locus_tag="ACHALG_002693"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFN2588007.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1289..1516)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1878..3080)
                     /locus_tag="ACHALG_002694"
     CDS             complement(1878..3080)
                     /locus_tag="ACHALG_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN2588008.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="ACHALG_002695"
     CDS             complement(3140..3343)
                     /locus_tag="ACHALG_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFN2588009.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4552)
                     /locus_tag="ACHALG_002696"
     CDS             complement(3485..4552)
                     /locus_tag="ACHALG_002696"
                     /inference="COORDINATES: protein motif:HMM:NF014537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFN2588010.1"
                     /translation="MLDSLQEEVVPVNRVLIDYLTIHFFSNQHLKLIEEIIEMPIERF
                     EELDYAPKGYIGQYVWNQVITIRYSIDDAVKGTVMEFSGQGCKHLARWLKTRKTNWQQ
                     FFRKVLDYQGNFTRIDFTLDDFVGSLSIPELKHKVILGHVWTTFQVSESHGGTDIINN
                     ESNGETLYLGSKKSQCRFCFYQKDHEQRKRRGIPLEEAKVKNRFELRYRKEKAQSLAK
                     IISKTHDLTKLFFELLNGAICFYDRDPNDLGAKVDAKWAAFISNYGAITISLDTIPQS
                     FEKTMSWLIHGVSPSLVFIAQVDQTFHSSLLPLIIEQGEMNPRQIKLLQNMKADPEYY
                     QEEVEYYERKLQSISNEKTSY"
     gene            complement(4610..4717)
                     /locus_tag="ACHALG_002697"
     CDS             complement(4610..4717)
                     /locus_tag="ACHALG_002697"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN2588011.1"
                     /translation="MNQVNQHDLGEAIRVSREERGWTQRYLAEKVGISR"
     gene            4880..5692
                     /locus_tag="ACHALG_002698"
     CDS             4880..5692
                     /locus_tag="ACHALG_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN2588012.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1748 a         1219 c          949 g         1996 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgggctct ataatttatg
     5881 ggactgatga atcagaattg attcttcagc cc
//
LOCUS       DAZABD010000109         5834 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000109 DAZABD010000000
VERSION     DAZABD010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5834
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.114"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(14..1096)
                     /locus_tag="ACHALG_002699"
     CDS             complement(14..1096)
                     /locus_tag="ACHALG_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HFN2588013.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            complement(1237..2505)
                     /gene="dltD"
                     /locus_tag="ACHALG_002700"
     CDS             complement(1237..2505)
                     /gene="dltD"
                     /locus_tag="ACHALG_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HFN2588014.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            complement(2508..2741)
                     /gene="dltC"
                     /locus_tag="ACHALG_002701"
     CDS             complement(2508..2741)
                     /gene="dltC"
                     /locus_tag="ACHALG_002701"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HFN2588015.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            complement(2786..3991)
                     /gene="dltB"
                     /locus_tag="ACHALG_002702"
     CDS             complement(2786..3991)
                     /gene="dltB"
                     /locus_tag="ACHALG_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HFN2588016.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            complement(3988..5502)
                     /gene="dltA"
                     /locus_tag="ACHALG_002703"
     CDS             complement(3988..5502)
                     /gene="dltA"
                     /locus_tag="ACHALG_002703"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047473 - D-alanine [D-alanyl carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HFN2588017.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            complement(5513..5662)
                     /locus_tag="ACHALG_002704"
     CDS             complement(5513..5662)
                     /locus_tag="ACHALG_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HFN2588018.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
BASE COUNT         1774 a         1204 c          959 g         1897 t
ORIGIN      
        1 attctattat tcattattca taactccgaa ttttttcaac tgtttcacta tcaagccgtt
       61 tcactaattc tgtgactagt ttcactgtat tcaaataatc atcttcatga atcactgatg
      121 tatgggaatg cagataacga acaggaacgg taatggctac agacggaaca ccatctcttg
      181 tcaaatgcat ttgtcctgca tcagttcctc cgccagaaat cactgtatat tggaatggaa
      241 tatccatttc ctctgctaca tcaataacaa aattcaataa ttttttatgc gggatcatag
      301 atgcatcaaa aatcaaaatc tgtgggcctt ttcctaaaat cgaatctgct tcttttggtg
      361 tcattcctgg agtgtctcct gctgtaccag tatctaaagc aaaagcaata tcgggattta
      421 caaggtgtgt acttgttcga gcacctcgca agcctacttc ttcttgcaca tcgcttcctg
      481 cgaataaaac atttgggtgt gcttctttag atagattctc taatactcgc agagaaacag
      541 ctgtcccgat acgattatcc catgcttttg ccagtaaata tttactgcca ttcattcgtt
      601 tgtattcaat atatggagtg accatatctc caggacgtat gccccactct ttggcttctt
      661 cttggcttga tgcgccaata tcaatgaaca tatcctttat ttcataaggt ttatttctta
      721 cttgaggtgt taaaacatgt ggtggtttgg aaccaatcac accgtggacg attttccctt
      781 cacgtgtttt gatttcaact tgctgagcaa gcatgacttg cccccaccaa cctccaagag
      841 tttggaattc caagaatcct ttttcggtta tttttgttac cataaaacca acttcgtcca
      901 tatgtccaga aataaagacc ttggggcctc cgccagcacc atgtttggca atcacactgc
      961 ctaaaccgtc ataaaaaata tcatcagcga atggtttggc atattctctg aatacttctc
     1021 ttacctcttc ttcatttcca ggtactcctt ttgcagaagt taaatcaata agtagctgtt
     1081 cttcttttgt atccaaatgt tttcccccat ttctatattc atattgtgat tataccattg
     1141 cttatgattt ttgttcaggc aaacttggta tttttatctt ttattcagaa tattcaaaaa
     1201 taaagaaaac aagcgagcaa cactcgcctg ttttctttat tttaattgta gtttatctgg
     1261 tatttgatgt tgccaagatt tgctaaaaaa agcatcgtca agatgatact tactagcagt
     1321 aatatgattt tcttctaaaa acggtctgat ctgttgatct gcagccaacc agcctttcca
     1381 gcctagatgg atcgtgtctt ccataaaata gttggtaccc gcttgattga caaaatcagc
     1441 tattcgattg aatccttggc tatttaattg aaatttgatc tttttggcaa atccttgtaa
     1501 catttcttga gatagtccgg tataatcgct ccatttctcg ttaaccggtg ggatgatgaa
     1561 caatacttcg ttatggtttt ttgctaattg atccaagacc agctgaaaat cagaaaattc
     1621 tggcgaaaaa cgataatccc aatttttttg tgactgtttg agctctggct cgtgggctcg
     1681 gatacgatgc gtataaaaat cgtttttcaa cccgaatgga ttattcgtcg tttctcgttc
     1741 tccaatttga tttgctaaaa tggataattc tgtttcttgg tattgttttg gtaatcgttt
     1801 tgactcatga tcgatttttt gctgtcgatc tttcaaccca atattgctga atacttcatc
     1861 ctctctcttc agcatgttcc attggctgcg cagctgattc aaagattcag gtaaaggtag
     1921 cttccctttt ttgatcgttt gcagaatcgc cgtcaacgta tcattctctt ttacttttga
     1981 gaaagttaat aatcttctag ctaaatagcg atcagcaggc gttactttct tcattgaaaa
     2041 caaccagtcg tatgtttgaa gttcagaata atacgtatta aagtaatcag tttttacacc
     2101 atttttaacg aaccactgag gagaaataat aaagacgact ttcttgtttt tcatagcatc
     2161 tcctgcagaa cgcatcatca tatattggct taacgattgt gtcccgggtg ctcccagcaa
     2221 aaatggacga tagtttcgct ggtatttttc tgccagaaca gacggatgaa atggactgat
     2281 tctgcttaat tcacttgagc caaaaaaagg aatatattgt ttttcagata atgcataatt
     2341 ttttatcgca tctcctttca acacgttcac tgacatggaa gaagaggcac gtctaatcgt
     2401 ttctggatta gagatatcta cttgatagga ggaggtaaaa aacacaaaca gtaagccaac
     2461 agctaaaaca accgggccga aaatagcaaa aagtttcttt ttcatactca acccttcaat
     2521 gcagttgctt gttgaatgat tttattcggt gtgctccaat cctctcgctc gaattcagat
     2581 acagggactt ggatatccag ctctccgtct agctcgacta ataattgaac agatgctaaa
     2641 gaatcaagca acccttcatc aaatagatca atatcacgat tttttcttac ttcatctgtt
     2701 cctgttattt cttctaagat tgataaaatt tgttcttcca ttttcctttt ctcctttgtt
     2761 atataaatta atatgtcact attcgttagc taaaaaaaag tttatctaaa aatcctgaaa
     2821 aaatcaggaa gctaaaacat actgcttgaa atgtcaaaaa tacggctaag ccatgcgtaa
     2881 atttgttaga aggcaactgc tctttatgct tcttcttaaa tcggatccat gcatctgtca
     2941 gacagatcag acaagcatga tacagtccat atacgatgta gtaccacgtc aatccatgcc
     3001 atatacccat tagaagaaac aaagcaaaat aaccgatatt tgacattacg atcttgcttt
     3061 taaaaaattt tttcttcatc atgaagaaca tcaatcgcat atatacataa tcacggaacc
     3121 aaaaggacag cgtcatatgc caacgattcc agaattcttt gatattccat gataaaaaag
     3181 gtttattgaa attgattggt gtctgatatc ccatcatgta gcttgtaccg actgcaaata
     3241 agctatagcc agcaaaatca aaaaacaaat acatactgta cacgtacata taacctacta
     3301 atgcccatga aataccacca tgctgtaagg caatgcgtcc cactacagga agcagtactt
     3361 gaccaaaata ataaccgata atgaatttat ataaaaaacc gacaaaaata ttttggacgc
     3421 cttttcccaa aagatcaaca tatttatcac gaacaggcgg atggagctga tctttttcaa
     3481 atctcctata gcgatcgatt ggtccagaag agattgttgg aaagaacagc agaaactgga
     3541 tataacgaaa tggcttatat gttttgatca tgccatctct tatttccatg atcatttgta
     3601 cagatttaaa ggttaaataa gagattccta aaaaaccaag taacgatgct tttccatgtt
     3661 caaaaaaagg agtcactttc gttaaaaaca gtggaattat cgctaaaata acggcggcaa
     3721 aaaaccatcc tgtctgattt ttcctttgcc gataaacaaa atagccccat acaagtatcg
     3781 tctgccacaa aacataccca atcagcgccg ctccctgttg ccaataatta cctccaaagc
     3841 tgacaaataa aaagaaaagt gtcactgctg attgataaac tggtaatctt ttttcccata
     3901 tcaataaaga tagaacgatc ggtacaagcg caatggctaa aagaaaaaaa taaaacggct
     3961 ctgcataagg gatcatatga ggtaacatca ttcaggattc acctcagctg ttaatttttt
     4021 tcgatcgatt tttccattaa ctgtctgagg cagcagatcg acataatgga acttctgagg
     4081 aatcatataa tccattacaa cttctagtaa ttgttctttg atagatttag tcaactgaaa
     4141 atccggctgt ttcgttttgt tttctaatac gacaaaagca accagctgct gtacttttgt
     4201 tccttggtat tttggtacga cgacagcttg tttgattcgg ttgtcttgga gcagataatg
     4261 ttcgatatcc ccgagttcaa tccggtatcc atgtaatttg acctgctgat ccatacgtcc
     4321 ttcataaaat aaaagcccat attcatctag tctacctgca tcccctgtat ggtaacttgc
     4381 taagtcattt tgacaaaaga acgcagcagc tgtcttttcg ggattgttca ggtaaccttt
     4441 tgatacgctt ggtcctgcaa taatgatttc acccgtttct ccttttgaaa caggtttacc
     4501 ttcttggaca atagaaactt tcgtgtcttt ttttacgtag ccaatcggta gtcggtcata
     4561 actatctagt acatgctggt caatctgaat acttgaaata gctacagttg cttctgttgg
     4621 accgtaagta ttatatatcc gtgcatttgg gaatcgatca agtagacttt ctgcagtttt
     4681 tttcgtcaat tcttctccgc aaaataaaaa catgtccaat tccggcacat tttctccatt
     4741 gaatgtaggt tccatcaagc aaatatccat aaatgatgga gtagaaaccc acacattcaa
     4801 cttcaaggct ggcaatgtcg cgaataattg acgaaagtct tgaacaactg ctttatttaa
     4861 cggcttcagc attccgccag aaactaatgc aggataaata gaaaataccg ataaatcaaa
     4921 agaaaatggc gcttgggcta agaaatgcat accaggcttg atcccaaagt cttccatcgt
     4981 ccattgaaca aagctgacta gattatcatg actgatttgt acgccttttg gttctccagt
     5041 agtaccagaa gtaaaaataa tataaaaatt ttctgtacct tttacgctat ttctgggata
     5101 aaatcctact ggtatcttct tgatttcttg aaattctttt tttgttatga caggaaccga
     5161 aggaatatct tctatccagt ccccgatact gatgaccata gatggtttag ccacccgaaa
     5221 gatcgatagg atacgctcct tgggtgtatg ctcttcaatc ggcaaatagg catggccaga
     5281 tttgacaacc cctagaaatg agacaatcat ttcaaactcc aaattgccaa aaacgacaat
     5341 tggtccatcg ccttctacct ttttttctaa ataataagca agtgcgtctg atgcttgttt
     5401 caattcacca aaagtatgcg tctcatccgc ctgataagca actcgatcag gttcatttat
     5461 tccccaatca tcgattgctt caataatcgt tttgatttcc atactcttcc tcctaaaatt
     5521 cgttatagat aaaagttcct ccttgaatat ttttatagtg atacagataa atcaggaaca
     5581 gcagtactgc actataaaaa attgttcgaa tgatgaattt tccccaataa ttcaattttg
     5641 gattagaacg ccatttgttc attggattcc ctcctcatat aacctcacac aaagtgtaac
     5701 taattttgac aaaaagaaac atgtgtctag cttacagaaa ccgttttttt actaacagtt
     5761 ttgtcacata gcttttataa aaatagcaca tcaaatcact gcttttttct ttataatttc
     5821 tcgtactttt aaaa
//
LOCUS       DAZABD010000110         5786 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000110 DAZABD010000000
VERSION     DAZABD010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5786
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.115"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..480
                     /locus_tag="ACHALG_002705"
     CDS             <1..480
                     /locus_tag="ACHALG_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFN2588019.1"
                     /translation="SIYPATITHSHKLMFEDRTIPLESYPTEQIIAEKLSATFDFLTD
                     NSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTRRGKTDPLKIYYEKDMDVLKNSHEL
                     KDTWKKYQKSNPYAASITYDETMDSISNLMEKVIHVEEKEQKLAYQRHQQMNREMER"
     gene            483..967
                     /gene="ssb"
                     /locus_tag="ACHALG_002706"
                     /pseudo
     CDS             483..967
                     /gene="ssb"
                     /locus_tag="ACHALG_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            989..1246
                     /locus_tag="ACHALG_002707"
     CDS             989..1246
                     /locus_tag="ACHALG_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2588020.1"
                     /translation="MAKKISLTMDLLIPVIAFIDGISLGVTENPLVNRIAKGSLFLVF
                     VYLVCSYLLKRAGKDNSFWRLTKVTVAATAVILTAILPLYM"
     gene            complement(1405..1539)
                     /locus_tag="ACHALG_002708"
     CDS             complement(1405..1539)
                     /locus_tag="ACHALG_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HFN2588021.1"
                     /translation="MLKSIKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK
                     "
     gene            1682..1942
                     /locus_tag="ACHALG_002709"
     CDS             1682..1942
                     /locus_tag="ACHALG_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2588022.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIESMQIRNPDHFTRSMGI"
     gene            2391..2597
                     /locus_tag="ACHALG_002710"
     CDS             2391..2597
                     /locus_tag="ACHALG_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HFN2588023.1"
                     /translation="MRKKFDYLGVPFSELFPNYHAPHTVECDCGERAKCIKSYCLYQC
                     PTCGKKYTLSYGDYILIEEKGKKR"
     gene            2597..2848
                     /locus_tag="ACHALG_002711"
     CDS             2597..2848
                     /locus_tag="ACHALG_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2588024.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGCELHYQENLETYENEYWLTKLEN"
     gene            2864..3301
                     /locus_tag="ACHALG_002712"
     CDS             2864..3301
                     /locus_tag="ACHALG_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2588025.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYNETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCFEIGVKPSTIKPSEYQALE
                     LTYAKFIETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            complement(3412..3639)
                     /locus_tag="ACHALG_002713"
     CDS             complement(3412..3639)
                     /locus_tag="ACHALG_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2588026.1"
                     /translation="MKQNTSITKNTARFNCTAALSRISISPTNSTNNKGIIFFKWITF
                     FLLSYRALPVYFAPPKRRGRLVRNKNHSLSY"
     gene            3737..3907
                     /locus_tag="ACHALG_002714"
     CDS             3737..3907
                     /locus_tag="ACHALG_002714"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2588027.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAKKKQDLRYQH"
     gene            3982..4377
                     /locus_tag="ACHALG_002715"
     CDS             3982..4377
                     /locus_tag="ACHALG_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2588028.1"
                     /translation="MKKKQTKLSLADILTQLTATEDGVVEFERSEISVVDDRYFKMPY
                     FFDQAKVICLCGYDGVRDYFGIRITEEKVVWVNNHTELGALAFEGTVLDNISIVFEEE
                     SFTLECDKLTRYIDPKFYEDKNLAWELAL"
     gene            4419..4625
                     /locus_tag="ACHALG_002716"
     CDS             4419..4625
                     /locus_tag="ACHALG_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2588029.1"
                     /translation="MKYNLLDHKRMWVEKLEEDLEQLELLGHNKTSTVYKEGLKRTKK
                     AQKELATLESRVQKKEVDLTHTYL"
     gene            4635..4868
                     /locus_tag="ACHALG_002717"
     CDS             4635..4868
                     /locus_tag="ACHALG_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HFN2588030.1"
                     /translation="MADKRKEKIQKLLALAADVNDEESVSALAKAQQLMMDYDLTEDE
                     IFNYHEQQRIEAVETHVIYRGRPQKWLYRLFRN"
     gene            5190..5393
                     /locus_tag="ACHALG_002718"
     CDS             5190..5393
                     /locus_tag="ACHALG_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucocin A/sakacin P family class II
                     bacteriocin"
                     /protein_id="HFN2588031.1"
                     /translation="MILGIVLLSVSTLGITVDAATYYGNGVYCNTQKCWVDWSRARSE
                     IVDRGVKAYVNGFTKVLGGVGGR"
     gene            5383..5574
                     /locus_tag="ACHALG_002719"
                     /pseudo
     CDS             5383..5574
                     /locus_tag="ACHALG_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177651.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5645..>5786
                     /locus_tag="ACHALG_002720"
     CDS             5645..>5786
                     /locus_tag="ACHALG_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFN2588032.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKW
                     VKQ"
BASE COUNT         2087 a          909 c         1175 g         1615 t
ORIGIN      
        1 tcgatctatc ctgcaacgat cactcacagt cataaattga tgtttgagga tcgaacgatt
       61 cctttggaat cctatcctac agaacaaatt attgctgaaa aattaagtgc aacctttgat
      121 tttttaacag ataattcgag aggaaaagat ttttatgatc tctatactat tcctaaaatg
      181 gagaagatcg atgagaagac cttgaaagat agtgtgagaa atacatttac acgtcgagga
      241 aaaactgatc cgttaaagat atactatgaa aaggatatgg atgtactgaa aaatagtcac
      301 gagctaaaag atacctggaa gaaatatcaa aaaagcaatc cttatgctgc atcgatcacg
      361 tacgatgaga caatggattc gattagtaat ttgatggaaa aagtgattca cgtcgaagaa
      421 aaagagcaaa aactagctta tcaaagacac caacagatga atagagaaat ggagagataa
      481 agatgattaa taatgtgaca ttagtaggac gcttaaccaa agatcctgat ttacgttata
      541 ccgcaagtgg tactgcagta gcgactttta ccctagccgt caaccgaaat ttcacgaatc
      601 aaaacggcaa tcgagaagcc gattttatta actgcgtgat ttggcgcaaa ccagcagaaa
      661 caatggcgac attagctaaa aaaggtagct tgattggtgt agttgggcgg attcaaacgc
      721 ggacttacga caaccagcaa ggacaacgtg tatatgtgac tgaagttgta gcagataact
      781 ttcaactatt agaaaataaa gcagcgacag agagccgtgc gcacgctgat caatcgtcca
      841 ccagtccaag caccgccacc tttgaacaac ggatacagcg acgcctaaca acaatggatt
      901 gaacgcttca cagaatccat ttggtggaca atcaatcgat attagcgatg atgatctacc
      961 gttttagttt gagaaagaag gagtaagcat ggcaaaaaaa attagtctga cgatggatct
     1021 attgataccc gtaattgcat ttatagacgg gatttctttg ggcgtcactg aaaatccttt
     1081 ggtaaataga attgcgaaag gatcactatt tcttgtattc gtgtatcttg tctgttctta
     1141 tcttttaaaa agagcaggaa aggataattc cttttggcga ttgactaaag tcacagtagc
     1201 agcgacagct gttatactta cggctattct accgctttat atgtagagtt aacgggaaat
     1261 cgttgaactt cttgcgaaat attgctataa tgaacatgaa aaagaggagt tgcgctaaca
     1321 acccctcatt aacagcaccg attaaagaac ggtggcagaa tattagttat ttggaaataa
     1381 ccgctgacct gctaaagtag acggttattt ctttttgtca tttttaagca tatttacaat
     1441 caaactaatc aaagcaatgg taaacatacc aaaaccaaga atggtttgaa ttgtctcata
     1501 tgcggacaaa aggcttactc cttttataga ttttagcact tacattcata agcaccacca
     1561 cctttccaga agtcgccacc gtcttcaaac tttactgtct tgactattat agcatatcta
     1621 aaatcgaata aaaattctca attttaactg tttttgctat aattttagcg aggtgatcgt
     1681 gatgaacaaa tatgttggtt tgcttgataa aatccgagta ataaaaacac aaccattatt
     1741 ggttcgcttt actttacaaa caattcatga atcgattaat tgtgtagttg cagatattga
     1801 aattatcgat aaattgttaa ttatggatga tggaaaatac aatattgccg tcacaggtca
     1861 tttcaataaa cggaatcaat tagtcattga atcgatgcaa attagaaacc ctgatcattt
     1921 tacgcgatca atgggcatat aaataaaaga agagctgcaa tcctaatgta ggactgcagc
     1981 tctttttgtt ttacatagaa acaggaagaa agggaaaggc ctgtttagga aggcacaagt
     2041 gccttctcaa ttgaaaaacc tttctctcac tccaaccgcc accgccgttc gagaagaaag
     2101 gaagtgaacg ttgcacgttc actaagataa ggggtcttgc tttgtaagac aaaataggaa
     2161 aagcttcctc tcaccaaaag gttcgttcct gttcgctatc tctttattga tttgccagac
     2221 cggcaacaag ttgcctttta tcaccaaaaa gcgacaagga caagggtaaa tggcacgctg
     2281 cgcgcccttg tccttgtcgc ttttcgttga ttcattctgg ccaacaaatc aataaagaaa
     2341 gaactaacga aaggaagtaa aacctttcct aagaggaggc agaatcaatc atgcgaaaga
     2401 aatttgatta tttgggtgtt cccttcagcg aattattccc aaactatcat gcaccacata
     2461 ctgtcgaatg cgattgtgga gaaagagcga aatgtatcaa aagctattgt ttgtatcaat
     2521 gtccaacatg cgggaaaaaa tataccttgt cttatggcga ttatattttg attgaggaga
     2581 aaggaaagaa acgctgatga aaacacaaca caaattattg actgtaaaag aagtcgttca
     2641 aattttggtg gacaaagaag tcatttctga aaaagtagca gaagctttac ttattccgcg
     2701 gtactggacg atagaaaccc ctttagtaga ggatgcaccg actatgctat ttgaagacct
     2761 agtgaagatt gcaggatgtg aactgcatta tcaagaaaac ttggaaactt atgaaaacga
     2821 atattggtta accaaattag agaactaaaa aggagaaaaa aatatggctc aaaattacta
     2881 tgacgaattt gtcaaattac ctttggataa aatggcacaa aaaatggagg atatgacgtt
     2941 cctatacaac gaaacacgtg ttccaaaaaa acattataaa gaaaaattgt cggtagctgt
     3001 ggaagaaatg attgaatcag gtgtcgaaat gaatcttatt gcgacgtact atcgaacact
     3061 ggaagaactg aaaaagcaaa atgcaaaatg gtttttccaa gccttactat gctttgaaat
     3121 aggtgtcaaa ccaagcacta ttaaaccttc tgaataccag gctttagaac taacgtatgc
     3181 gaaatttatc gaaacgaaaa aagccaagac tgtttcaagt gaatggttag attattttga
     3241 aaatatcaat aagtatggcg catattacac catgaaaaag gaagacaacg aaaatgaata
     3301 aggtgattta tcaactaaca acggattcca tatcaacgat ttgaaagaaa aaaaggtcaa
     3361 taatcattta taccgaaaag tgagaatgtg tggaataagg acaaaaaaag cctagtaact
     3421 aagggaatgg ttcttatttc gcaccaaccg cccccgccgc ttaggtggtg cgaaataaac
     3481 gggcaaagcc cgataactta agagaaagaa ggtgatccat ttgaaaaaga ttattccctt
     3541 attgttagta ctgtttgttg gcgaaatact tatgcgagat agtgcagctg tacagttaaa
     3601 cctcgctgta tttttggtaa tactggtgtt ttgtttgatc ttttggcggt ttgtattagt
     3661 cctcgtgagt ctactattca agatgattgc agccatattt ttcatggggt tatttcgatg
     3721 aaaggagaga gtaccaatgc caattgcaat aggaaataaa cggttacccg tgacattaga
     3781 tgaaaagaga caaaaagaat tgcagcaact aaagcagaag tacggcaaaa gtgaatccag
     3841 gattatgtgt attgcgttag atttattgat tgccaagaaa aagcaggatt tgaggtacca
     3901 gcactaagaa agtgacgtca ctttttatac taaaacctaa aagtgatatc acttttagac
     3961 aatggaaaag gtggaaataa gatgaagaaa aaacaaacta aactatcgtt agcagatatt
     4021 ttaacacagt taaccgcaac tgaagatggt gttgtagaat ttgaacgatc tgaaatttcg
     4081 gtagtagatg atcgttactt taaaatgcct tacttttttg accaagcaaa ggtcatttgc
     4141 ctttgtggct acgatggggt acgtgattac tttggaatta gaattactga agaaaaagtc
     4201 gtttgggtaa acaatcatac agaattaggt gcgctagcct ttgaaggaac tgtattggac
     4261 aatatctcta ttgtattcga ggaagaaagc tttacgcttg aatgcgataa gttaacccga
     4321 tatatcgatc caaaattcta tgaggataaa aacctggcat gggaactggc tttatagaca
     4381 gagtgctagt gcaagtaaag gaagaaagga agatcaccat gaaatacaat ttattagacc
     4441 ataagcgaat gtgggtagag aaattagagg aagacttaga acagttggaa ttgttaggtc
     4501 ataataagac ttcaacggtg tataaagaag gactaaaacg aacgaaaaaa gcacaaaaag
     4561 aactagcaac actggaaagt agggttcaga aaaaagaagt agacttaaca cacacttacc
     4621 tttaggagta gaaaatggca gacaagagaa aagaaaagat tcaaaaatta ctggcgcttg
     4681 cggcggatgt aaatgatgag gaatcagtga gcgcattagc caaagcccag caattgatga
     4741 tggactatga tcttactgaa gatgagattt tcaattatca tgagcagcaa cggatagaag
     4801 cagtagaaac gcacgtgatc tatcgtggac gtccgcaaaa atggctgtat cgtttattta
     4861 gaaattaaga taaaaaaggc tagttaacag gtttgttgac tggccttttt tgatatgttt
     4921 gtacatgttt tattgtgttt taatttttct cttttttata gcaagctcct tttataataa
     4981 atattccgaa aagtgaagac cattttttat ttattaagac tattattatt agtgagataa
     5041 aatttgattg atcttacaga atattggtga cgattatgac actaaagata ctatttatga
     5101 aaattttgca aaaatgttct gaaaattaca tataattgtt ttataaaaaa gggaggcaat
     5161 tatatgaaga aaaaatttgt tagtattttt tgattttagg aattgtttta ttgagtgtat
     5221 ctactttagg aattacagta gatgctgcaa cttattatgg aaatggtgtt tattgtaata
     5281 ctcaaaaatg ttgggtagat tggagtagag ctcgctctga gattgttgat agaggagtaa
     5341 aagcttacgt taatggtttt acaaaagtgc taggtggagt cggtggaaga taaaaaagaa
     5401 aaactttttg acttaattgc gaatgcatat aattcaaagt catataataa tgtagaagta
     5461 aaagaaggat ttcttcaaga tgctaagaaa atacgagagg ataaaccata tcctgaaatt
     5521 attatatcag tgtacaaaca tttatctaaa ttaaaagctg aggaacgcat ggataggcta
     5581 caattaacta gacagaaaaa ttaaggtgtg tagactagaa gaaaacatac caggaggaat
     5641 ttttatgtct aagagaacac gaagaacttt ttcacaagaa ttcaagcaac aaatcgtcaa
     5701 tctttactta gctggaaagc cacgtgtaga aatcattcga gaatatgaac taacggcttc
     5761 agcatttgac aaatgggtaa agcaat
//
LOCUS       DAZABD010000111         5691 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000111 DAZABD010000000
VERSION     DAZABD010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5691
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.116"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(490..1299)
                     /locus_tag="ACHALG_002721"
     CDS             complement(490..1299)
                     /locus_tag="ACHALG_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFN2588033.1"
                     /translation="MVKESVIGKKELRSIFWRSFFMQSSWSFDKMMAYGQMNGIERSL
                     RKIYPNDDDYYTALQRHTETFNITPHIAPFVMSLSVTMEEENAKNPEFDTDAINKIKT
                     GLMGPLSGIGDSFFWGTFRVIAAGIGISFAQQGNILGMILYFTLYTAIHFIFKWLATK
                     YGYVLGTKFIDDTEDNHMIDKLSHGASILGLTVIGAMIGTMVTLNTSLKFTVGGAETS
                     LQSIFDQIFPGLLPLGITFLCVWLYSKGHKTITIILGIFVLCFLGTLGGIF"
     gene            complement(1299..2045)
                     /locus_tag="ACHALG_002722"
     CDS             complement(1299..2045)
                     /locus_tag="ACHALG_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFN2588034.1"
                     /translation="MIQAILIALVAFLASIDEYTFGASMMGRPLFTAPFVGLILGDLE
                     SGIIIGATLESMFMGSIMVGSATPPEVYASSVLGTAIAIQSGQGVGTAVALTLPLSIF
                     LQMWRNFAYAMPITYFNNKMDEALVNHDFKKAQKYHYVAQPLIVGVPSAILVFVATYF
                     GVSTINAMIKLIPQVVLDSFDVAAGVLSCVGLALLIKMMANKAMMPYLFLGFVGVMYL
                     KMDVVGVAVVGLCLALLAVQKLTFEEEEDL"
     gene            complement(2063..2530)
                     /locus_tag="ACHALG_002723"
     CDS             complement(2063..2530)
                     /locus_tag="ACHALG_002723"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFN2588035.1"
                     /translation="MIKLLRVDERFVHGQVAFAWTNNLGANCIFVASNEIATNNLRKK
                     TLKMAVPTGTKFVVKDMTEAISVLNGEKIKKYQVFLIVGTVEDAYEITQHVPEIKEVN
                     LGNLKKNENRKMITSSVYLNEQELDLVKKMTENGITVQCQAVPTDKKINPLHD"
     gene            complement(2530..2940)
                     /locus_tag="ACHALG_002724"
     CDS             complement(2530..2940)
                     /locus_tag="ACHALG_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFN2588036.1"
                     /translation="MRKIVIATHSMMAKGLKDTLEFIIGPQKSLLAITAYIDSDYSIN
                     QEIEQLFSDLAPDDELIVTVDLLGGSVSNAFSEQIGKDNFYLISGVNLPLLLELCLSG
                     EQDTKKLIQKAMNSGNEGIVLVNELLEKTEEDDF"
     gene            complement(2951..3964)
                     /locus_tag="ACHALG_002725"
     CDS             complement(2951..3964)
                     /locus_tag="ACHALG_002725"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFN2588037.1"
                     /translation="MLGFSEERFLSVLNGAVKNIETINQVADAISEKGFKNLYLVGSG
                     GTYATISPLAYLLSTHSELDYYHEIAAEVVCSQPKKINKDTVLITSSLTGTTQETVDV
                     VKYANSQGATTIAFVGNAATPLGEIVDYAIENDADNDTLVEELQLQFFALGARLMKNN
                     GEFPEYDEFIQTLQKMPEVLLSVRQQNDQKALEFAEKHKNTEFHMFVGAGNTYGAAYE
                     YAMCVLEEMQWIATKSIHAAEFFHGTIEMTDKSMSFVLLKGEDQTRPLVDRVERFVNK
                     YSDVVTVFDTADFELAGVPSAMRQYVAPMVMATALERVSAHFEKVRDHSLDIRRYYRT
                     VEY"
     gene            4181..4915
                     /locus_tag="ACHALG_002726"
     CDS             4181..4915
                     /locus_tag="ACHALG_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFN2588038.1"
                     /translation="MNTEELTNKLIQDIEQEKYKYAQAIPSERDFVQIYEMNRSAVKK
                     AIQQLVNKEYLLKIHGKGTFVKKNIHEHLQIKFHGLSELLEREGIQPSSLILASEIKP
                     AGHIVGKALQLEPTEEVFHLVRLRSGDGEPISIEDTYIPLNLIPDLQAFDFQIFSFYS
                     TLAAYHYQIENIYQTISSSKVSNKEARLLKTSLNRPVISLAITAVTTNEQAVEYTEVV
                     VLPEFAAIYTKNVHQGKEMKIYAQID"
     gene            4899..5609
                     /locus_tag="ACHALG_002727"
     CDS             4899..5609
                     /locus_tag="ACHALG_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFN2588039.1"
                     /translation="MPKLINLLAYDLIDKIIAYIHEHHLTPGTKLPSERDLSEMFNVN
                     RTTLRGALQKLVDNGTLRAQTNSGYYLTAPKFEKNAHHFFTPIEDSLLSKQRLQLKAT
                     ALSPKKAAALCPELNDFKQQQPQAYLEYIDAEAISLMLLIPMETLKKSNFSDNPFQDF
                     KSLAYTRSQEISLITDNDVAADLLGVDKEEVLLFVREEIRAGKELAAIALGYTISRRC
                     NIQVNVATTLQSGSVAKF"
BASE COUNT         1833 a         1195 c          943 g         1720 t
ORIGIN      
        1 taaagcgaca aaaaccacga gcaggtccat tcatcctcaa acctcggtga tcttcgcggt
       61 ttaacaccat cagaagaaat gcacaaaaat aataaggaga gtctgagaca taaataaatt
      121 cccagacttt tagtgagtct aaatccgaat aaacggtggg tcgaaagcaa ctctagctgc
      181 gcgtcgatcg cagcaatctc taggtgctat gttcggataa atggtggtca aaagtcagat
      241 tttcggccat ttcgtaacag ccaaccagac aaaaagcgtc tgttttgtct tctactccaa
      301 ttgctcaaat cttagcgact tttggcttgg tcttactcta aacagtttgt aagcctaaac
      361 tttatttgct gtttgtttct aagatcaaag caaaactact ttttagttgg ttacttttac
      421 aagttctttt tactgttgaa taaatgtaaa tcttaagaac aaatatttac ttctataatt
      481 ccaagtttct taaaaaattc cgccaagtgt acctaaaaag cataaaacaa aaattcctag
      541 aataattgtg atcgttttat gccctttgga gtaaagccag acgcataaaa aggtaattcc
      601 taatggcagc aagcccggaa aaatttgatc aaaaatactt tgtaatgaag tttcagctcc
      661 accaaccgtg aattttagtg acgtatttaa agtaaccatc gtgcctatca tcgcgccaat
      721 taccgttaaa cctaaaatcg aagcaccatg agataatttg tcaatcatat ggttgtcttc
      781 tgtatcatca ataaatttag ttcctaaaac atagccatat tttgtcgcca accatttaaa
      841 aataaaatga atggcagtat acaaagtaaa ataaaggatc atgcctaaaa tattcccttg
      901 ttgagcaaaa gaaataccta tgccagccgc aataacccga aaagttcccc agaaaaatga
      961 atcgccaata cctgataaag gtcccattaa tcctgttttg attttattaa ttgcgtctgt
     1021 atcaaattca ggatttttag cattttcttc ttccatggtg acggacaagc tcataacaaa
     1081 aggcgcaata tgcggtgtaa tgttaaatgt ttccgtatgg cgctgcaacg ctgtataata
     1141 atcatcatcg ttcggatata ttttacgtaa ggaacgttca ataccattca tttgtccata
     1201 tgccatcatt ttatcgaagg accaagagga ttgcataaaa aaggagcgcc aaaatatact
     1261 tcttaattct tttttcccaa taacactttc ttttaccatt ataaatcttc ctcctcttca
     1321 aaagttagct tttgaacagc cagtaacgct aagcaaagtc cgacgacagc cacaccgaca
     1381 acatccattt tcagatacat tacgccgaca aatcctaaga aaagataagg catcatcgct
     1441 ttattcgcca tcattttaat taataacgct agcccaacac aagaaagcac accggccgca
     1501 acatcaaagc tgtccaatac aacctgagga atcaacttga tcatcgcatt gattgtagac
     1561 acaccaaaat aagtagctac aaaaactaaa atggccgatg gaaccccaac gattaaaggt
     1621 tgagccacat aatgatattt ttgtgctttt ttgaaatcat gattgactaa tgcctcatcc
     1681 attttgttgt taaaatacgt aattggcatt gcataggcga agttacgcca catctgaaga
     1741 aagatggaca aaggtaaagt caacgcaacg gcagttccta ctccttgacc cgattgaatc
     1801 gcaattgctg tacctaaaac actggaagca taaacttcag ggggcgtcgc actccctacc
     1861 ataattgagc ccatgaacat ggattccaag gtggcaccaa taataattcc tgactctaaa
     1921 tctcccaaaa tcaaaccgac aaaaggcgct gtaaatagcg gtctacccat catcgatgca
     1981 ccaaatgtgt attcatcaat agacgccaaa aaagctacca gtgcgattaa tattgcttgt
     2041 atcatattct tcccctcctc ttttagtcat gcaaaggatt gatttttttg tccgttggta
     2101 cagcctgaca ttgaactgtt atcccgtttt cagtcatttt tttcaccaag tccagttctt
     2161 gttcattcaa gtaaacggaa ctagtaatca ttttccgatt ctcgtttttc tttaaatttc
     2221 ctaaattcac ttctttaatc tcaggtacgt gctgagtaat ttcgtaggca tcctccaccg
     2281 tcccgacaat taaaaatact tggtatttct tgattttttc tccatttaaa acagagatgg
     2341 cctctgtcat atccttcacc acaaattttg tccctgtagg aacagccatt tttagtgttt
     2401 ttttgcgcag attattggta gcgatttcat tacttgcaac gaaaatacaa tttgcaccta
     2461 agttatttgt ccaggcaaaa gcaacttgcc catgaacaaa acgttcatct actcgtaata
     2521 atttaatcat taaaaatcat cctcttctgt tttctctagc aactcgttca ccaaaactat
     2581 cccttcattc ccgctattca tagctttttg gattagtttt ttcgtgtcct gttcacctga
     2641 taagcaaagc tctaaaagca gcggcagatt tacaccgctg attaaataaa aattgtcttt
     2701 accgatttgt tcggagaaag cgttagacac gcttcctccc agtaaatcta ccgtcacaat
     2761 taattcgtca tcaggcgcta agtcggaaaa tagctgctct atttcctggt taatggagta
     2821 gtcactatct atatacgcag ttattgctaa aagtgatttt tgaggaccaa taataaattc
     2881 taaagtatcc tttaagcctt tagccatcat ggaatgcgtc gcaattacaa ttttcctcat
     2941 agctcttacc ttaatattcc acagtccgat aataacgacg aatatctagt gaatgatccc
     3001 gcaccttttc aaaatgagca ctcactcgtt ctaaggccgt tgccataacc attggcgcta
     3061 catactgacg catagcgctt ggtacacctg ctaattcgaa atccgcagtg tcaaaaaccg
     3121 tcacaacatc gctatattta tttacaaaac gctctacgcg atcgactagc ggacgcgttt
     3181 gatcttcacc ttttaataac acaaaactca tagatttatc agtcatttca atagtcccat
     3241 ggaaaaattc ggctgcatgg atcgacttgg tggcaatcca ttgcatttct tccagtacgc
     3301 acatagcata ttcataggca gccccataag tattcccggc tccaacaaac atatgaaatt
     3361 ccgtattttt atgtttttct gcaaattcaa gtgctttctg atcattttgt tgccgaacac
     3421 ttagtaatac ttccggcatt ttttgtaatg tttgaataaa ttcatcatat tcaggaaact
     3481 caccgttatt tttcatcagg cgtgcaccca aagcaaaaaa ctgtaactga agttcttcta
     3541 ctaatgtatc gttgtcagca tcattttcaa tcgcataatc cacgatttcc ccgagtggtg
     3601 tagcagcatt tcctacaaac gcaatcgtcg ttgcaccttg actattggca tacttaacaa
     3661 catcaacggt ttcttgagtc gtaccggtta atgaagatgt gattaacact gtatctttat
     3721 taatcttttt cggctgtgaa caaaccacct ctgcggctat ttcatgataa taatcaagct
     3781 ctgaatgagt gcttaacaga taagctagcg ggctgattgt ggcatatgtt ccaccagagc
     3841 ctaccaaata aagattttta aatcccttct cactgattgc atctgccacc tgattgattg
     3901 tctcgatatt ttttactgca ccatttaata ctgatagaaa acgttcttcg ctaaaaccta
     3961 acatagttaa aaacctccaa gtcaataatt tgaaaaatat atttgcaaat gccggcacat
     4021 taacaaacca cttaaccttt tttatatacc acctgttaac gtttttaatg taccacttat
     4081 agaaaacgct gtcaatagtt tttaaccact tttattttta tttgtaccaa aatccttttt
     4141 tcatgtataa taaacaaaaa tagactctgg agtgtaacgt atgaatactg aagaactaac
     4201 gaacaaatta atacaagaca ttgaacaaga aaaatataaa tacgcgcaag ctattccttc
     4261 agaacgtgat tttgttcaaa tttatgaaat gaatcgttca gccgtaaaaa aagccattca
     4321 acaattagta aataaagagt acctactaaa aatacatggc aaaggaactt ttgttaaaaa
     4381 aaatatccat gaacatttgc aaatcaaatt tcatggtttg tctgaattgt tggaacggga
     4441 agggattcag cctagcagct taattttagc ttcagaaata aaaccggctg gacacattgt
     4501 cgggaaagct ttacagctgg aaccaactga agaagtattt catttggttc gtttaagaag
     4561 cggcgacggt gagccaattt ctattgaaga cacttatatt ccattaaatc tcatacctga
     4621 tcttcaagct tttgatttcc aaattttttc attttattcc acattggctg cttatcatta
     4681 tcaaattgaa aatatctatc agacaatttc cagctcaaaa gtgagcaata aagaagcccg
     4741 tttgttaaaa accagcttaa atcgtccggt aatcagcctt gctatcactg cggttacgac
     4801 aaatgaacaa gcggttgaat acactgaagt agtggttttg cctgaattcg ccgccatcta
     4861 tactaaaaat gttcatcaag gaaaggagat gaaaatctat gcccaaattg attaatctgt
     4921 tggcttatga cctgattgac aaaattattg cttatattca tgaacatcat ttaacccccg
     4981 gaacaaaact gccttcggaa cgggacctat cagaaatgtt taatgttaat cgaactacct
     5041 taagaggcgc gttacaaaaa ctggtggaca acggcacctt acgggcgcaa accaatagcg
     5101 gctattattt aactgcgcca aaattcgaaa aaaatgccca tcatttcttt acaccgatag
     5161 aagattctct gcttagtaag cagcggctac agctaaaggc aacggctctt tcaccgaaaa
     5221 aagcagcagc tctttgtccg gaactgaatg atttcaaaca gcagcaacca caagcttatt
     5281 tagaatatat tgatgcagaa gcaattagct tgatgttatt aatccctatg gaaacattaa
     5341 agaaaagtaa tttctcggac aatcctttcc aagattttaa aagtttggca tatacgcgtt
     5401 ctcaagaaat ttctttaata actgacaatg acgttgcggc ggacttgctg ggagttgaca
     5461 aagaagaggt tctgttattt gtacgagagg aaataagagc cggtaaggag ttggcggcga
     5521 ttgctttagg ttatactatt agtcgccgct gcaacataca ggttaatgtt gctacgacct
     5581 tgcaatccgg ttctgttgca aagttttaaa tctactatca gataaggtag aataatagaa
     5641 aaagatagca ggaggaatga cgatgagtca ttttaaagga aagcaatttc a
//
LOCUS       DAZABD010000112         5176 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000112 DAZABD010000000
VERSION     DAZABD010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5176
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.117"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(136..1098)
                     /locus_tag="ACHALG_002728"
     CDS             complement(136..1098)
                     /locus_tag="ACHALG_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN2588040.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1100..2539)
                     /locus_tag="ACHALG_002729"
     CDS             complement(1100..2539)
                     /locus_tag="ACHALG_002729"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004564 - beta-fructofuranosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFN2588041.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2724..4670
                     /locus_tag="ACHALG_002730"
     CDS             2724..4670
                     /locus_tag="ACHALG_002730"
                     /EC_number="2.7.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
                     /protein_id="HFN2588042.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKGTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            4934..>5176
                     /locus_tag="ACHALG_002731"
     CDS             4934..>5176
                     /locus_tag="ACHALG_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFN2588043.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPK"
BASE COUNT         1666 a          878 c          855 g         1777 t
ORIGIN      
        1 tttctccact ccaaaaaagt ctaacttttg ggggtcacta caagttgaca actccttttt
       61 gtttacaaat caattggata tctacaaatt gtattttcta acgaatttta tagacattaa
      121 aaaattaatt taacgttaaa tacttcctcc agaaagtatt gagattggta aaatataatc
      181 cttatttgtc ttttcgcctt taattttttt cagaagaata tcaacaataa gacttgcaat
      241 ttcatcaatt ggttgtctta ttgttgtcaa ttgtgggaaa aatttctcaa taaaagaggt
      301 accatcgtat ccaataattt ttaaatcttc tgggatatta agtttaagtt gatgagcaat
      361 tttcatagtg agaatggctg ttaaatcatc agaaacaaaa ataccatctg gtttgtgatt
      421 tataatagtt gactttattt ccatttcacg acggatagta gacagattat taggaatttt
      481 gaaaattttt ccatttggat tttgaaaaga aaaacctagt gcacgtaatt ctgtgggaga
      541 atccgtgtta tcatttcctg taatcataat ggtattttga caaccatttt tttgtaaagt
      601 cttagctgca agttttcccc cttcaaagtt atctgaagaa acaatggaaa cattaggagc
      661 taaatttcta tcaaaagcaa caataggtgc tctcacacgc tcataatcag caattccaag
      721 attatggctt gatgagatta tgccatcaac ttggttggct tcaagcattt caatgtattc
      781 tctctcttta gcaggatcat tttgactatt gcaaataatc gttttatatc catgtttaaa
      841 taactctatt tcgagatact caatgagttc agagtaaaag atattactaa tattaggaaa
      901 aataagacca ataagctgag gtgactttcc ttgaagtcca cgtgctaaat tatttggttt
      961 ataaccaaga gttttcatag cttcatgaac tttttgtttt gttttttctg ataaatatcc
     1021 cttattatta atgacgcgtg aaacggttgt tgggctaact ccagctaatt cagcgacatc
     1081 agttaatttt gctaccattt taatatttta attcaaagta ttttccagta ggttcgcctg
     1141 atttgatttg aataccattt tggtgatttt cagggaatac acgactagta aacacttttt
     1201 ctcctttatt aataaaaatc tcaatgattg agttatctac aaaaatgtta gcagttgttt
     1261 ctttagtatc aattgagcat tcacgaatcg taccaaagtc agtggcatat tgaatgcctg
     1321 ctttgctgcg atctaaaata atttttcctt ctttggtatc tacaatcaag ctaagtccat
     1381 ttcctttact atcagagaaa agaagtaact ccgatttctg atttggagga aaagttagtt
     1441 caagctcata agtattattt gtttctgctt ttgctgtaaa ttgttcagaa ctagcacgta
     1501 atgatgtaat agcttctact ggatattggt aaagtttacc atcttttatt gaaagttctt
     1561 ttacaagact cattgctccc tgataatcat acttatcagt tggataatcg atatcaggta
     1621 gtccaatcca actgacaatc agagcacgac catcaggagt attgaatcct tgagtcgcat
     1681 acgcttcaaa tccataatca agattttgaa tttctgaagc gcccacaagt tttacttttt
     1741 ctatgtcaag cgattgacag atcttaaaag tattcggata gatattccca taatttagct
     1801 cagatttatc caacccttga ggacaataaa tgagaacagg ttttttgtct acaaatacta
     1861 gatttggaca ctcaatcatg tattctgaac cagtgccgcc aaaatcaagg ttgccaactt
     1921 cctcccatgt ttccacgtta ttgtcatccg ctttgtaaag tttgataaag cctgattttt
     1981 ctaaattttg tgctccaaca atcgcataca attgtccttc ataattaaac atttgtggat
     2041 cacgaaaatg atcagttaca tcagcaggtt tatgaataag aacattctca cctttcgtaa
     2101 tgttgtagtc tttatccatc catgctccga tttgaagtgt atcacgttcc caattttcat
     2161 ttcgaacgtt tcctgtatag agtaagaata gattcccctc tatttcatat gcagaaccgg
     2221 aataagcgcc gtggctatct cgttttgtgt ctggaagaag ttctgctcct gttacatgaa
     2281 aatgaactaa atcatctgat tcattgtgta cccattcttt caacccatga gcagctccaa
     2341 atggccaatt ttgataaaaa agcgtatatt ttccgttaaa aaaagaaaaa ccattaggat
     2401 cgtttaacaa tcctgtttta ggttcgatat gatagctagc acgccaaggt gatcgtttta
     2461 cattatcctt aattttatcc atctcatctt tagtccaatc agaataagca cgatatcgta
     2521 tttctcgagg taagttcata ttttactcct tcaatttatc tttttacaac tagtatggta
     2581 aacgttttcc gttgaaaaat caatcatttc atttaaaaaa ataaactata tgacaaacgt
     2641 ttgacatatt tttaagatat gctaaaataa atttgcattt cagtgaaccg ttttcacgta
     2701 aaaataaaaa aggagtgatt cgaatgaatt acagtaaagt agcttccgac gtaatacaag
     2761 cggtcggaaa agataacctt attgcagctg cacactgcgc tacacgtctt cgtctcgtac
     2821 taaaagatga cacgaaagtg aatcaaaaag ctttagacga aaatccagat gtgaaaggca
     2881 catttaaaat tgatggacaa tatcaagtga ttatcggtgc aggtgatgtt aatttcgttt
     2941 atgacgaact tatcaaaaaa actggacttt cggaaatttc aacagatgat ttaaaacaaa
     3001 ttgctagtaa agataaaaaa ttcaacccca ttatggcatt aattaaatta ttatcagata
     3061 tttttgttcc aattatacct gcccttgttg caggtggttt attaatggca ttacaaaact
     3121 ttttaacagc tgctggactt tttggaccaa aatcaattga agaaatgtat cctgcgattg
     3181 aaggtatttc ctcaatgatc caactgatgt ccgctgcacc attcatgttc ttaccaattt
     3241 tagtaggtat ttcagctgct aaacgttttg gagcaaacca atttcttggt gctgcaattg
     3301 gtatgatcat gacaacacca gatcttggtg gctcaactga atattggaat atttttggtt
     3361 atcatgtttc acaaacaaac tatgcttatc aagttattcc agttttagca gctgtttgga
     3421 tattatctat attagaaaaa ttctttcata aaaaattgcc atcatctatt gactttacat
     3481 tcacgccatt attatcagta atgattactg gatttttgac tttcacagtt attggccctg
     3541 taatgttatt gttgtctaat ggaattacag acattattgt ttggctatat aacactactg
     3601 gatttattgg aatgggtatt tttggaggaa catattcact tatagtcatg actggtttac
     3661 atcaatcatt tccagctatt gaaactcaat tactttcagc gtggacaaat ggtatcggac
     3721 atggagactt tatctttgta gttgcatcta tggcaaacgt tgctcaaggt gctgcaacat
     3781 ttgccatttg gttcttaact aaaaattcta agacaaaaag tcttgcttcc tctgcgggtg
     3841 tctcagcact tcttggaatt acagagcctg ccttgttcgg agttaactta aaataccgct
     3901 ttccattctt ttgtgcttta attggttcag gaattgctac agcagtagca ggattactta
     3961 aagtagttgc tgtttcactt ggttcagcag gtttcttggg atttctttca attaatgcaa
     4021 catctattcc attttacgta ttgtgtgaat tgattagctt tgtagttgct tttgcaatta
     4081 cttacttcta cggtaaaaca aaagctgctg gtatctttaa agcagaagaa attgctgaac
     4141 aaacaccagt taattcttca aaagtagcaa caacagatcc aatatctact gatgttgaat
     4201 caacacaaac aaaagtttca tcagaaacaa ttgttagtcc catttctggt aaagtgatta
     4261 gtctttcttc agtaaatgat ccagtctttt catcagaagc tatgggaaaa gggattgcca
     4321 ttaaaccaaa tggaaacacg gtccattctc cagtagatgg tactattcaa ctcacgtttg
     4381 aaactggaca tgcttatggt attaaatcaa ataatggtgc tgaagttcta attcatattg
     4441 gtattgatac tgtatcaatg ggaggaaaag gatttacaca aaaagttact actaatcaaa
     4501 aagttagaaa aggcgatatt cttggagcat ttgaccgttc aatgattgag aaagaaggtc
     4561 ttgatgatac aacaatgatt attgtgacca atacttcgga ttattcagaa gtaaatatag
     4621 tagctgaaaa tacagtcact gagggtactg atttacttga aatcaaataa atatatttcc
     4681 ctgagtaaag atggaatttt ctttccaaaa tcaaataata tagattaatc aaatatttga
     4741 gaataacatt ttatttaatc ttttcatata tcaattgtat gaaaaattat tagaatttta
     4801 aaaaatgata aaaattcaaa aagaaaaaat agtagttatt tacttgtcat gttactaaat
     4861 aaaaaaataa tttaaatatg atatatagtc atttatttag tgatttcatt ctaaaataaa
     4921 gggagaaatg tctatgctat acggtgcaat tgaagcaggc ggcacaaaat ttgtgtgtgc
     4981 cattggcgat gaagaaatga cgatcaagga aagagtgagc tttccgacaa cgacacctga
     5041 ggaaacaatg cctttagtca ttgacttctt caaacaatac caagcagatt tagcagggat
     5101 tgggattggc tcatttgggc ctatcgatat ccatcgcgat tccgccactt atgggtacat
     5161 tacctcgacc ccgaaa
//
LOCUS       DAZABD010000113         5104 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000113 DAZABD010000000
VERSION     DAZABD010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5104
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.118"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            53..664
                     /locus_tag="ACHALG_002732"
     CDS             53..664
                     /locus_tag="ACHALG_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFN2588044.1"
                     /translation="MYFILPTLYSLLCVMAPCIIYMVFFCKKNTLKHNILILIFMLYC
                     WKVFELTGTGGLTDIFYRPDTGRKFSLVQGSVNLVPFSGLNISFILNIVMCVPLGFLL
                     PIIWKSYRKIVPVFLAGALFSLIIEVSQIITTRATDIDDLIANTFGAVIGFILWAVLS
                     RVVSLKDKSSTVDYLEPYIYILIGFTGKFLLYFPFWFAFEVLS"
     gene            737..1666
                     /locus_tag="ACHALG_002733"
     CDS             737..1666
                     /locus_tag="ACHALG_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HFN2588045.1"
                     /translation="MKRFLKWGLVLLASLIVILMCTAVVISYVNRPYARAVYDRIKRI
                     LDKDFYDHLYLGSSFEIINLDTMDIHSYMKKDQEELPASLSKLFTIDYALSVLSLDEQ
                     ITISPTALDMQKADSSTAHLQEGDILSVYDIICAMVLPSGNDAAYSLAIYTGGKLDPD
                     AKNDEERYQSFMKNLDRYLSEQGYKHTHFTDAAGYDSNATTSAEDLTKVAAKLLKNDK
                     FAEIVAKETYRAKSIKGNVYNWNTTNQFMQQDNRYFTEGVKGVKTGSFDGRYNLCVYY
                     TSSSDNHYLLVSFGSESDSRRYVDVQNLLHNLP"
     gene            1803..2561
                     /locus_tag="ACHALG_002734"
                     /pseudo
     CDS             1803..2561
                     /locus_tag="ACHALG_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033645.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
     gene            complement(2609..3811)
                     /locus_tag="ACHALG_002735"
     CDS             complement(2609..3811)
                     /locus_tag="ACHALG_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFN2588046.1"
                     /translation="MHRKEDFHMNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKT
                     ELTAFLDYEKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYR
                     RTNDTLEETVIHLFRKGITMSEIADLIEKMYGHHYTPQTMSNMTKSFTEEVTSFKERE
                     LHDRYAAIYMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEI
                     LIDLQERGVKNVLLFITDGLKGMAGAVQRFYPKARFQHCCVHVSRNISHKVRVDDRKE
                     VCDDFKMVYQASSKKAALEARGAFAEKWKTSYPKMVESILSNDYLLTFYDFPLAIRKT
                     IYSTNLIESFNKQIKKYSHRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTD
                     TLASMFTE"
     gene            3891..4415
                     /locus_tag="ACHALG_002736"
                     /pseudo
     CDS             3891..4415
                     /locus_tag="ACHALG_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(4622..>5104)
                     /locus_tag="ACHALG_002737"
     CDS             complement(4622..>5104)
                     /locus_tag="ACHALG_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFN2588047.1"
                     /translation="KEAIDQIKTAVLYPHNGLPILLMGVSGSGKTFLAEKIYEYAKEQ
                     QVIESNAPFIAYNCAQYFNFSINKIVENLTILDAMKVLRLVQKAVKELEKLMGIEFSN
                     NQLFLLYMHCCSMIERILRKESVDEQADIKEYIQKEGHNYEISKYPAYKTKFESTVIK
                     "
BASE COUNT         1605 a          868 c          990 g         1641 t
ORIGIN      
        1 gaagtttata tgaagttata aaatttaatt tatattggaa ggaggaaaat aaatgtattt
       61 tatactacct acactttata gccttttatg tgtaatggca ccatgtatta tttatatggt
      121 atttttttgc aaaaaaaata ccctgaaaca caatatcctg attttgattt ttatgctgta
      181 ctgttggaaa gtttttgaat tgactggtac aggtggacta acagatattt tttatagacc
      241 tgatacaggt aggaaatttt ctctagtaca gggcagtgtt aatcttgtac cattcagtgg
      301 actaaacatt agctttatat taaatatagt aatgtgtgtg cccttaggat tcttactacc
      361 aatcatatgg aaaagttata ggaaaatagt tcctgttttc ttggctgggg ctctattttc
      421 actaattatt gaagtctcac aaattataac tacaagagca acagatattg atgatttaat
      481 agcaaatacc tttggtgcgg ttataggctt tatactgtgg gctgttttaa gtagagtggt
      541 gtccttgaag gacaagtcat ctacagtaga ttatttagaa ccttatattt atatattgat
      601 tggttttacc ggaaaattct tactgtattt tcctttctgg tttgcttttg aagtattatc
      661 ctgataattc catagaaaat aatgagtttg agttgagaaa gaatattctt gtacacaact
      721 ctggaaagga ggaagtatga aaagattctt aaaatggggg cttgttcttt tagcgtcttt
      781 gattgtgatt ctaatgtgta cagcggttgt tatttcttat gtgaatcggc cgtatgcaag
      841 agctgtttat gataggatta aacgcattct tgacaaagat ttttatgacc atctatatct
      901 aggttcttca tttgaaataa taaaccttga tacaatggat attcatagct atatgaaaaa
      961 agaccaagaa gaattacccg cctcattgag caaattattt acgattgatt atgcgcttag
     1021 tgtcctaagt ttagatgaac agattacaat tagtcctact gcacttgaca tgcagaaagc
     1081 tgactcatct actgcacatc ttcaagaagg agatatttta tctgtttatg acattatctg
     1141 tgctatggtc ttaccctcag gaaatgatgc agcttatagt ttggcaattt atacaggtgg
     1201 aaaacttgat cctgatgcaa agaatgatga agaacgctat caaagcttca tgaaaaactt
     1261 agatcgttat ctaagtgagc aaggctataa acatactcat tttactgacg ctgctggcta
     1321 tgactccaat gctacaacat cagcggaaga tttgacaaaa gtagctgcaa agctactaaa
     1381 gaatgataaa tttgcagaaa tagttgccaa ggaaacttat agagcaaaat caattaaggg
     1441 aaatgtttat aactggaata ctacgaatca atttatgcaa caagataata gatactttac
     1501 tgaaggtgta aaaggtgtaa aaactggatc gtttgatggg agatacaatc tatgtgtgta
     1561 ttatacatct agttctgata accactattt acttgtaagt tttggttcag agagtgatag
     1621 taggcgttat gtagatgttc aaaatctatt gcataatctg ccttgaaatt tcgtgccttt
     1681 aagcgcgtat ctaaagtttc tttgaaaagt ttgagaatga agaaagtgag cagtcgaaaa
     1741 acggttgctc acttttttgc cctaaaatcg aaaaaatata aaaaagtcct ttacaacacg
     1801 tctcaggaaa aactgctaag tcaattctaa tatcttgtgg agcaagactt gcaccttttg
     1861 atataagaga gcttagagaa ctaatgagcg aagatgaact agaactcgat aaaattggag
     1921 atagaaaaac tgccttgttt gtaataatat cagatacaga tgatacattt aattttgtag
     1981 tttcaataat gtattctcaa ttatttaatc tactatgtga taaggcagat gatgtgtatg
     2041 gtggaagact tccagttcat gttaggtgtc tacttgatga gtttgcaaat atcggtttga
     2101 ttcctaaatt tgaaaaacta atagcgacaa ttcgttcaag agaaatatcg gcaagtataa
     2161 tactgcaagc acaatctcaa ttaaaagcaa tttataaaga ccatgcagat acaatagttg
     2221 gtaactgtga ctctacactt tttctaggag gaaaagaaaa aacaacagta aaagaattat
     2281 cagaaacctt aggaaaagaa accatagacc tatataacac atcagaaaca agatcaaatc
     2341 aaaaatcttt tggtctaaat tatcaaaaga ctggcaagga acttatgagt caagatgaaa
     2401 taactgtaat ggatggtggt aagtgtattt accagctaag aggagcaaga ccttttctat
     2461 ctgataaatt tgatattaca aagcataaga attacaaatt gttggaagat tatgataaga
     2521 aaaacttgtt tgatgtagaa gagtatttaa aacaaagata gcgtaaaata ttttgtgtaa
     2581 ataactcgtc cttctgcaaa ataatgggtt actcagtaaa cattgaagct aatgtatccg
     2641 tcacttgttg gaagccttta tggcttctat ttaagaattt ttgattgtat gtatcaaaga
     2701 tggatactag gaaacgctct agtgattctt cattttgaaa ctgctctttt ctgtggctgt
     2761 atttcttgat ttgcttatta aaggattcaa tcaagttcgt agagtaaata gtcttgcgta
     2821 tggctaatgg aaaatcatag aaagtgagca ggtaatcgtt cgaaaggatc gattccacca
     2881 tttttggata actggttttc catttttccg caaaagcacc acgtgcttcc aatgccgcct
     2941 ttttagatga cgcttgatac accattttaa aatcatcaca gacttcctta cgatcatcga
     3001 cacgcacttt gtgactgata ttacgggaaa cgtgcacaca gcaatgttga aaacgggctt
     3061 tggggtagaa ccgctgcacg gcccctgcca tcccctttaa gccatccgtg atgaaaagga
     3121 ggacgttttt cacgccacgt tcctggaggt ctattaaaat ctcttcccaa atcgtgatag
     3181 attcagttgg tgcaatcgca tagctcaaca cttcctttga accgtccggg cgaatgccaa
     3241 ctgcgatatg aatggcttcc ttggcgacgg ttttccgctt taaagggata taggttgcgt
     3301 ccatataaat agcggcataa cggtcatgaa gctcacgttc cttaaatgaa gtgacttctt
     3361 ccgtaaatga tttagtcata ttggacatgg tttggggcgt gtagtgatgc ccatacattt
     3421 tctcaatcaa gtctgcgatt tccgacatgg taataccttt tcggaagagg tgaatgacgg
     3481 tctcctctaa ggtatcattc gtccgtctat aagcaggaac agtctgttgc ttgaactcgc
     3541 cgttgcggtc gcgcggaatc tggagatgaa gttccccgta ctcggtcttg accgtacggt
     3601 cataggagcc gttacgagaa ttacccgtgt taaaaccaat gcgatcgtac ttttcgtaat
     3661 cgaggaaagc cgttaactcc gttttgagaa gcgtattgac agctttttcc aaatggacac
     3721 ggaataattc attcaaatcg cctttattcg ctagagtctt tagaatttct gtagtaaaat
     3781 cgttcatatg gaagtcctct tttctgtgaa tgtgttgtgg taactctatt ctactgaagg
     3841 gacttccttt tttctattta cacaaaatat tttacactct cggattgaaa gaacgcggtc
     3901 tttcaggagt aaagatggtc atctcggatg cgcatatagg gctcacaaaa gctgtgaaag
     3961 aataccttct ttgtgcgagc tagcagagat gccaagcaca tctccttcgc aacatttccg
     4021 acaaattctc caagaaaatc tcaagcgata tcaaagaaga ctttaaagcc atcttcaaaa
     4081 cggcaagcat cgagctggct ggagaacgta aacaacttat tatggataaa tatggggaag
     4141 acaagaaagt cgcaactgcc tgtaaaatat tggatgaggg cttcgaagat gccatgcaag
     4201 taatggtttt accagaaaat atccgtcaac gattgagaac aacgaatgtt cttgaaagac
     4261 taaatgaaga agtacgccgt cgtgaacgtg ttgtccgaat tttcccaaat tgtgattctg
     4321 ttattcgtat catcggtgct ttattgatgg aaaaggatga cgaatggact tctgcaccta
     4381 gaaaatatct tgaatttgat cgtaacgaga tttagcacat taagccgcca atagtataat
     4441 atactggtga gaagtatcaa gagacgcttc gctttttgct cttgacactt cttaccagca
     4501 tattggaccg tagctaggct caacaaggca aaacatcgta ctttgagatt tcctttttac
     4561 acaagatttt ggacttgaca cagcccctat ggttcttacc ttgtttatgc ttatttaaat
     4621 cttactttat tacagtactt tcaaacttcg ttttgtaagc cgggtatttt gatatttcat
     4681 aattatgtcc ctctttttga atatattctt tgatatctgc ttgttcatct acactttctt
     4741 tgcgtaaaat acgttcgatc attgagcagc agtgcatgta aagcaagaac aattgattgt
     4801 ttgaaaattc gatccccatt agtttttcga gttctttgac ggccttttgt accaatctca
     4861 aaaccttcat tgcatcaagg atcgttaaat tttctacgat cttgttaatc gagaaattaa
     4921 agtattgagc acaattatag gcgataaatg gagcatttga ttctatcacc tgttgttcct
     4981 tggcgtattc ataaattttt tctgccaaaa aggtttttcc tgaaccgctt acacccatca
     5041 ataaaatcgg taatccgtta tgaggataca agacagccgt cttgatttga tcgatcgctt
     5101 cctt
//
LOCUS       DAZABD010000114         5052 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000114 DAZABD010000000
VERSION     DAZABD010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5052
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.119"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..340)
                     /locus_tag="ACHALG_002738"
     CDS             complement(<1..340)
                     /locus_tag="ACHALG_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFN2588048.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGIT"
     gene            436..780
                     /locus_tag="ACHALG_002739"
                     /pseudo
     CDS             436..780
                     /locus_tag="ACHALG_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
     gene            811..1131
                     /locus_tag="ACHALG_002740"
     CDS             811..1131
                     /locus_tag="ACHALG_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HFN2588049.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(1160..1624)
                     /locus_tag="ACHALG_002741"
     CDS             complement(1160..1624)
                     /locus_tag="ACHALG_002741"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN2588050.1"
                     /translation="MVTYNWIKLREHPELVKQSAKWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(1753..3002)
                     /locus_tag="ACHALG_002742"
                     /pseudo
     CDS             complement(1753..3002)
                     /locus_tag="ACHALG_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
     gene            complement(3031..3348)
                     /locus_tag="ACHALG_002743"
     CDS             complement(3031..3348)
                     /locus_tag="ACHALG_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFN2588051.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(3374..3694)
                     /locus_tag="ACHALG_002744"
     CDS             complement(3374..3694)
                     /locus_tag="ACHALG_002744"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFN2588052.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDIINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(4095..>5052)
                     /locus_tag="ACHALG_002745"
     CDS             complement(4095..>5052)
                     /locus_tag="ACHALG_002745"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFN2588053.1"
                     /translation="DRYGDRVKNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNY
                     HKVLATAKAVEIYRSGNYGGRIGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDP
                     MVKGYYTEEFLELCQKQSIYFDPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNE
                     ETPFHPERYYEVFDLPGKKMNNSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGR
                     EHEERYMDEKGIVQDDYRIEFIGQHLRYLIKATEEGANCEGYMLWAFTDCVSPMNAFK
                     NRYGLVRIELNDERSRTLKKSASWYQTLIDKRELDLPDEIYK"
BASE COUNT         1536 a         1059 c          863 g         1594 t
ORIGIN      
        1 tggtaatacc ttttcggaag aggtgaatga cggtctcctc taaggtatca ttcgtccgtc
       61 tataagcagg aacagtctgt tgcttgaact cgccgttgcg gtcgcgcgga atctggagat
      121 gaagttcccc gtactcggtc ttgaccgtac ggtcatagga gccgttacga gaattacccg
      181 tgttaaaacc aatgcgatcg tacttttcgt aatcgaggaa agccgttaac tccgttttga
      241 gaagcgtatt gacagctttt tccaaatgga cacggaataa ttcattcaaa tcgcctttgt
      301 tcgctagagt ctttagaatt tctgtagtaa aatcgttcat atggaagtcc tcttttctgt
      361 gaatgtgttg tggtaactct attctacaga agggacttcc ttttttctat ttacacaaaa
      421 tattttacac tctcagaaaa agcgaaaaat ttagtaagct tacaaaacgg cacaatctct
      481 ccattacaga tggcatcgca agctgtcaaa aaatttggaa cagtccaagt aactggtgtt
      541 tatatgactc cagcttcttc ttacccgctg caagaatttt ttatgcggga tatcgctgtc
      601 aagcatggac aggcaccaat tatccatcta atgccaaaaa tttacgaaat gattgcagaa
      661 ggactattcg atcctacaca aatcattact cattcgctgc cactatcaga agcagcacaa
      721 gcatatgaga tctttgacaa aaaagaagat aaaaatataa aagttgtttt gaaaccttga
      781 attcagtaaa aaatacaggt gattttcttt atgcccaaaa tcaaacgaaa tacccatgag
      841 atcgaaatag gaaaagaaga agatatcgaa atacgaggac gtcgcttccg cttctatttc
      901 caaaatcgtt acacattgat ctcactggct gtcgatttat taaccgggat tttctatatt
      961 tttggaagcc ttgcttcctt aactaaaatc cccgatcgat atggcaccta tttttatttg
     1021 gctggtgcta ttttcttgac gattcgtcct attctcagaa tcgtaaggaa catttttatt
     1081 tacgacgaaa aaaaagcaat gaagagaaca agcaaaaaaa aagaggcatg agatttgaaa
     1141 aaatctcatg cctctttttt caccctatcg tatcactttg atacagacga gcggtcgtcc
     1201 cactttcttc cttgaccatt gtcaaaaatg tccactcgta tcgttcataa aacgtggtat
     1261 gatctgtaat taagtaaagt ttttcgtacc caagttgatg catttctttt ctgatttccg
     1321 ctaatatccg cttggctatc ccctgcttac gcttttccgg ttcaacaaac agggcacaaa
     1381 gatttggtgt caaatcaggg cggttatgga agtcgttttc aataacgccc gctcctccga
     1441 cgatggtttc tttttcatct aagaccacat accattgagg aatactttcc ttattagcga
     1501 ttccagtacg aatactttcc tcatacacaa cagcaggaac tccccatttt gatgaaaacc
     1561 acttcgcact ttgttttact aattctgggt gttctcttaa tttgatccag ttatatgtaa
     1621 ccaatgttta aacctccgac taaaataaaa cgtttacaaa aattatacaa gtctttatga
     1681 aaattagcaa tgacaacaat agtgatgtaa aaaaaacgga aaacaatcac ttgttctccg
     1741 ttttttgatt aatcaagcat ctaaagcttc ttttcttctt tcttggatcg tagctacctt
     1801 gacgaacggt agatagatca aaatagaaag aaccaaattg acagcggcta gtacaccacc
     1861 tgcaatactt tttgttgcca atacaccgcc aataattggt ggagttaccc atggaggcat
     1921 aacggtagct gctggcacta aacctgtaga agtcgcaaag taagcaacac taactaagac
     1981 cattggtgtc aaaataaatg gaatgaacat gatcgggttc aacacgatag gtaacccaaa
     2041 catcgttggt tcattaatat tgaagacacc tggcgcaatc gacaagtttg ttacaaccat
     2101 atatggtttg tttttacgtc ctactaaata aatagctaac agtaaaccta aagttgcacc
     2161 tgttcctcct aagttgacaa agctgtcaaa gaaaggtttg ttcacgataa acggtgcgct
     2221 atgtccagct gcgattgcat taacgtttgc ttcaatggct ggcgcattga ttgtttgcat
     2281 caaaggatcg accatattcg ctccgtgtaa accaaagaac cataagaatg gtgtgataaa
     2341 tgcaagcagt aaggcagatg gataagagtt tgcaagtccc ataaatggtt cttggaccac
     2401 tgtatagaaa gaacctacga tatcatcaac accgaatgca gcaaaaattg cagcaactaa
     2461 agcaaacgca gagatagtga tcattgctgg tagtaatgaa gcaaatgctt ttgcaactgc
     2521 cggtggcaca ccatcaggca ttttgatgat caatttttca tttccgctta aacgtacaaa
     2581 gatttctgtt gaaataatcc caacaataat agcaataaat aaaccgtttg agctcatacc
     2641 taatgctccg cctaaagcga agaaagagcc taaagaaatc acacctgcgg aaacaccgtc
     2701 ttttcggtag ccgttagcta aatgataagc aagaaagcaa tcaagacaga aagaatggca
     2761 aatgtaccgt tccaagcagc agccccgaat cctttccatg attcaccacc aaaaattgag
     2821 ttcatcaatt cttggaaccc gggaatcggt aagttattga tcattactgc taatgctcct
     2881 aagatcatta acggcatact caccataaat gagtcccgaa tcgcaactaa atgccgttgt
     2941 gcaccaattt ttgatgctac aggtataaaa tgtttctcca taaatgcaac aaatttatcc
     3001 ataaatcttc cctcttttct ttactgtaga ttacgcgttc aatcgacgat acagatcaat
     3061 gatctctttt gctaaatctg taaaagctat ggatgtcatc agatgatctt gactgtggac
     3121 tgtcaaaagc gtgacttcaa cgtgatttcc ttgtgcttct tgtgtcagca tctctgtttg
     3181 agagtgatgt gcttggacta aagattcctc tgcatctttg attttttcat ctgctaaagt
     3241 gaaatcacct tttttcgcag cagcaatcgc ttccattgca tcgctttttg catttccgcc
     3301 aaacatgatc agacccatga tggcttctag atttttttga tcttccattt attgtatcct
     3361 ccgataattt ttattatttg tttattagtt tttctgcttg gtcgagtact ttttcaccat
     3421 tcatcatgcc atagtcagcc atattgatta tatctaaagg aatgccttta ggtgctagct
     3481 tttcagcaaa ttgattttgc ataaagcgta cttgcggtcc taaaagtaaa acgtctacat
     3541 ttttagattc tagttgatta tccgcatctg aagcagatac agcaaagatg tctgcttcca
     3601 ttccttttgc ttctgctgcc ttttgcattt ttgtgactag gagacttgta ctcattccag
     3661 ctgaacatac aagcatgatt gttttctttt ccattttaaa cacgttcctt tctatactta
     3721 cacttgtata taagcaagta gcgtgccaac tttgaaagcg ctttaaaaag ttgatatcat
     3781 cacatttaag gtctatacca agttgcatac acactaaaaa agaaagtgca cactaaacac
     3841 acaaattaat ttttattacc ttttttcttt taacgtgcac aaaaaaagtt gtgacacaaa
     3901 acttgtcaca actttttttc gaataaacgg tattcaaaag cttgcatctg cggtaataag
     3961 tccaaccaag ttaaaaacat aagatgttgt ttttactttg ctattcttat tacttgatgc
     4021 aggacagctt tttcacaacc tcttttattt caaataacca gtaaatacaa tgaacatcta
     4081 caaaaaactt ctatttattt atagatttcg tcaggcaaat ccaattctct cttatcaatc
     4141 aaagtctgat accacgaagc agattttttc aatgttctgc ttcgttcatc gttcaattcg
     4201 attcgaacta atccatatcg attcttaaat gcattcattg gtgacacaca gtcagtgaat
     4261 gcccataaca tatagccttc gcaatttgcg ccttcttctg ttgctttgat caaataacgc
     4321 aaatgctgtc cgataaattc gatacggtaa tcatcttgca cgattccttt ctcatccata
     4381 taccgttctt catgttctct tcccataccg ttttctgtta tcagccaagg gatattatga
     4441 taattttctt ttagatacat ggctatatca tacatgattt tcggatagat ttcccatccc
     4501 ctggaattat tcattttctt cccaggtaaa tcaaagactt cataatatcg ttctggatgg
     4561 aaaggtgtct cttcattcca ttgataacga ggagcgcgaa cgcgtttagg ataatattga
     4621 ttgattccaa ggaaatctac tgtatgatgc tgaatcgttt ctaaatcctc tgttgcagga
     4681 tcgaaataaa tcgattgttt ttgacacaac tccaggaact cttctgtgta gtaccctttt
     4741 accatcggat caaaaaatac tcgattataa aacaaatcat acatataagc agctttttgg
     4801 tcttctggag aattagatcg agcatagacc atttcaggat ttaaaataca gccaatcctt
     4861 ccaccataat ttccgctgcg gtaaatctct actgctttag ccgttgctaa aactttatga
     4921 taattccaca tcatccactt ttttgtgttt tgttcatgcg gccagcgaac agcatccaaa
     4981 tagcaacgcg tttggggaac gattggttcg ttgaatgtaa accagttttt taccctatca
     5041 ccgtaacgat ca
//
LOCUS       DAZABD010000115         5047 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000115 DAZABD010000000
VERSION     DAZABD010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5047
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.120"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(80..1888)
                     /gene="pepF"
                     /locus_tag="ACHALG_002746"
     CDS             complement(80..1888)
                     /gene="pepF"
                     /locus_tag="ACHALG_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HFN2588054.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(2064..3062)
                     /locus_tag="ACHALG_002747"
     CDS             complement(2064..3062)
                     /locus_tag="ACHALG_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA family protein"
                     /protein_id="HFN2588055.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(3288..3941)
                     /locus_tag="ACHALG_002748"
     CDS             complement(3288..3941)
                     /locus_tag="ACHALG_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HFN2588056.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            complement(4273..4671)
                     /gene="spxA"
                     /locus_tag="ACHALG_002749"
     CDS             complement(4273..4671)
                     /gene="spxA"
                     /locus_tag="ACHALG_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SpxA"
                     /protein_id="HFN2588057.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT         1497 a         1038 c          767 g         1745 t
ORIGIN      
        1 aaaaaatacg tcgactccct tacaagagag ccgacgtatt tttttcgttt ctattcgaga
       61 aaatcaaaaa tagaaagcct tataaacgat cgatcaattc ttctaattca tttagacgtt
      121 gttcaaacat cgacattgca tcttcaatat atgcagcctg tgtcatatca actcctgctt
      181 ttttcattac ttccacagga taatcgctgt tacctgcttt taggtaagcc aagtagttct
      241 ctagtgcctc tggttcctgg tttagtattt tcttcgccag tgctgaagca gcagaaaagc
      301 cagtcgagta ttggaaaaca tagtaattat aataaaaatg cggtatccgt gaccattcga
      361 atttaatttc tgggtcttct tcgactgctg gaccataata ttttgcattc aatttaccat
      421 aactatcact taggtattca ctagtcaatg gcacaccttt ttcatcttcc gtatgcatga
      481 aatgctcgaa ttcagcgaat tgtgtctggc ggaaaactgt tcctttaaac ccatccaagt
      541 aatggttgag tacataagcc cgtacacgag ggtctttttc tgtttccaat aaatactccg
      601 ttaggatatt ttcattcgtt gtcgaagcaa tttctgctaa aaagatggag tagtcgccgt
      661 acacataagg ctggttcgaa cgagtgaaat aactatgaac actatgtccc atttcgtgga
      721 caagcgtaaa tagctgatcc agcgtatcat gccaattcaa taaaatatat ggatttgtgt
      781 catagcttcc cgaagaataa gcaccgcttc gtttcccttt attttcgaca acatcgatcc
      841 aacgttcaga gaatgctttt tctacgatgg ccatgtattc ttcacccatt ggtttcagtg
      901 cttctaaagc tttttctttt gcttcttcgt acgtaaaggt aattggagct tcccctaaga
      961 ccggtgtata aagatcatac atgtgcagtt tttccacttc taataaacgt ttccgtaatt
     1021 ccatgtatcg atgtaacaaa ggcaaatgct tgtttaccac gtctaccaaa gtatcgtata
     1081 cactttcagg aatatgatta ttgctcaaag acgcttctct ggcagagctg taattacgga
     1141 ctttcgcttt aaaattatgt ccttttatat gtgtgcctaa agtagaagca aatgtatttc
     1201 taaattgttc gtaaacactg tacaacccct taaatgctgc ttcgcgcacc cttcgatccg
     1261 tgctttctag caactgacca tacacgccat gagataattg gactatttca ccattttctc
     1321 cttcaatcgt tggaaaaact agatctgcat tattcaaaac agcaaatgta tcacttgaag
     1381 catcaaagat ttcacctgct ccagcaagaa gagattcctg ctcagcagat aagacatggg
     1441 ctcgattatc tactatttgc tggatataat ggcgatagac ttccaatttt ggttcttctt
     1501 tgaaatattg ccaaatctgg tcatctgaca attgcaatat ttctggttca aaccaggaaa
     1561 cggcttcact aactttcgaa aacagactgc ttgctcttgc ataaagcgct tggtaatctg
     1621 tatttccagt gtcttgatcg tttttaaggt gcgcatatac ataaatgact tcagtttggc
     1681 gataaaccct caatacgaat tcaatcgcat ttaaaaattg agaagcgcct tgatcaagtg
     1741 ttcctttgtg tttttcagat tgttttaact cttctgataa ttccaaatat ttctcatcaa
     1801 actcttggtc gcttgaaaag atcttggtca agtcccaagt taaattttca ggcaattctt
     1861 ctcgttttgg caactgcttt acttccatga aatccactcc ttttcttccc ttagctctat
     1921 ctatcaaaat agatcatagt ctaacatcca catgtcacag catctaactt ttctactcat
     1981 tcatgtgtct gcttccatca attttagcat aactaaatct tgttttacat agaaaaccga
     2041 ttcctagcct atttttatac ttactatttt ttcaaagaag acaagtgtat gcattcgtta
     2101 aaaaactgct gatagaccat ctcttcatca atcataggaa aaagccattc ccttttatgt
     2161 tcttttattt ggcaaagcca tgagttgaaa tcaggaactt tctggttttc caaggcttct
     2221 tcgtacaaca ttcgataaag aaaaacctgt tcttgtaaat aaaagaaaaa ccgacttttt
     2281 tgatagatcc acgatgatag atgcaaaaga tgtttatgac gcaagtagca ttgttcttgt
     2341 atggatactg tattcttttg cttgtgaata agttggcgtg tcagccattt cttcccatct
     2401 tcactcactt tttctatttt tttcatttgg ttcattttaa tttcgttact agtgaatatt
     2461 tctttaaggt ccctactaga aaaagtccag cagctactgc aaaaggaaat atgccctgat
     2521 acagtctctg taatatgatg atacaaataa agcttttgtt tcttgagatc agctttccag
     2581 caatgcactc ctctttttct attatagtag cagcaacttt tttctatcaa tgagaattgc
     2641 gattgtccaa gaaaagaatt accaagtatc caccatactt ggtaactgtt attcaaataa
     2701 ttttgggttc tttcaataaa tctctggtga gacagtctag aacattggat ttctattgct
     2761 agattcgcca acaaaatatc cggcctttgc cgcaacctcg gcaaataggc ttccaaaaat
     2821 actggttcta ctgactgtcc cagccaatcc atgaagcatt cctttaactg taagtgctct
     2881 tttgattctc tttcactaaa gaccgaacag ttatctgatt tttgatgggc aaaatgagca
     2941 cattttttct ttcccttttt caaaatcact ctttttcgac agccacgaca ataatatttc
     3001 tttccttttt ctgcatgttt agcaaaacag cacttattat attcattcat tgcaagcagc
     3061 atttttctcc ctctgaactt atttacgtct aagaatttat ttacacgaaa aaagagggcg
     3121 tgaaaaagct attctgcatc aagtaataag aacagctaaa caaaaatagt tcccaatatt
     3181 tttcgtttgg ttgggcttgt taccgcagat gcaagctttt gaacacattt atacgaaaaa
     3241 aagactgcga cacaattttt gtcacagtct cttactataa tgctgattta tacaaaataa
     3301 aatttcgttc gctcgatagc attacgcgcc atcagacatt gtccatattc attcaatgtt
     3361 tctggtgtac gatcgctctt ttcagcaaac tccaaaattc gagcaatctg attctctaca
     3421 tccgcttggt tgagattttc catccagaag tgaacgacta ggtaatagcg ctcttcgaat
     3481 aaatacaaat cagtccaagc agactctagt tcgacatctg ttgcaagttg gatcacttgt
     3541 tcaaaatctt caaaagcaaa agttgcttgt ctttcctgtt cttctagggc gcttccagat
     3601 aattcttcct ccggatcgct gccaataatc tgctgacgaa gaaagtcact cacatcttct
     3661 gaatcaacag tagaatcttc tgattgagaa atttcttcat ccggaagatt tttactgata
     3721 aacagttcta atccgtctcc ttttggcaaa acttggaaag tgacagcttc gcttcctcgg
     3781 aactcctctt cgatatcaac ttcttccaaa atactataaa agaaattttc gatttctttg
     3841 tgattgccaa gtaaatccaa aaatgaaacg ccccgttcct ctaaatcttc atgaccaatt
     3901 agtacacgga tcgtattttc attaatatgt tccatttcca ttttgctaca cctcactttg
     3961 ccacaatgtt taatatagat tcattgtagc gaaatcagct taaatgtaaa gaaaaaaaat
     4021 ctcgctgtta agcgaattta ataaaaccag cggatttttc aacctttcaa gctatcttat
     4081 ttaactataa catttttggc agacgaatac tatatagccg acttttcagc taattttccc
     4141 tctctatttt tcgtgcaatc atcatcttct actacctttt tatacatcta atcaagaata
     4201 ctttttgtaa aaacaaaaaa acttggaaaa actatctttg taatagtctt ttccaagttt
     4261 cgtattatcc ttctataatc cagccatcaa ttgtgcttga cgtaattcta attgtcgtac
     4321 atcgcgcggt aggaaccgac gaatttcatc ttcattaaaa ccaacttgca gacgtttatc
     4381 atcaatcatg attggacggc gaagcaatcc aggattgttt tgcacaagtt ccaataattc
     4441 ttgtaacggc agatcatcta aatccatgtt cagtttttgg aatactttgg aacgtgtcga
     4501 gatgatttct tctgttccgt cttctgtcat ttgtaaaatc gcttttaatt cagaactgtt
     4561 caatggttct gaaaaaatat tacgttcaac aaatggaatc tgatgttctt gcaaccacgc
     4621 acgagccttt ctgcaggaag tacaacttgg ggaagtatat aatgttaaca acgtgcacca
     4681 ctccttttcg tgataagaat cacttttttg taacagaaga tcataataat ctcccatctg
     4741 tttctattat acataatcaa cttttaaata gctacctttt tttcataatt tttttaaaaa
     4801 aatgaaagtt atctaaggtt aatatacagt aaaaccagca ttctcgcttt ttttatcatt
     4861 taaaaattag agttttcaaa tctaattcaa attttctaaa catatgaaac agcgtataaa
     4921 actgtactat tattttatct caaaagctaa atgaaaccga tttcttaaga ttttttatgt
     4981 tttttatttc tttaatagta atgttttctg tcattttaaa taatactgtt ctttattata
     5041 acgctta
//
LOCUS       DAZABD010000116         5038 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000116 DAZABD010000000
VERSION     DAZABD010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5038
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.121"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..355)
                     /locus_tag="ACHALG_002750"
                     /pseudo
     CDS             complement(1..355)
                     /locus_tag="ACHALG_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            425..586
                     /locus_tag="ACHALG_002751"
     CDS             425..586
                     /locus_tag="ACHALG_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFN2588058.1"
                     /translation="MQDKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPV
                     DAISYGVRK"
     gene            605..1882
                     /locus_tag="ACHALG_002752"
     CDS             605..1882
                     /locus_tag="ACHALG_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN2588059.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            1892..2548
                     /locus_tag="ACHALG_002753"
     CDS             1892..2548
                     /locus_tag="ACHALG_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN2588060.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            2548..3924
                     /locus_tag="ACHALG_002754"
     CDS             2548..3924
                     /locus_tag="ACHALG_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN2588061.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            4138..4467
                     /locus_tag="ACHALG_002755"
     CDS             4138..4467
                     /locus_tag="ACHALG_002755"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFN2588062.1"
                     /translation="MQDVLKDVFGPLFEKMLQGELNNHLGYDAHSKEPKKHDNRRNGY
                     GNKTLKTSFGKVNIDVPRDREASFEPELIPNRKRDVSDIADCRQKLFQTRNSSLEQLF
                     IYFVSNF"
     gene            complement(4390..5038)
                     /locus_tag="ACHALG_002756"
                     /pseudo
     CDS             complement(4390..5038)
                     /locus_tag="ACHALG_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element ISEfa7 family transposase"
BASE COUNT         1875 a          642 c          891 g         1630 t
ORIGIN      
        1 tgacgagaaa tatttagaat tttgcacatc gctgatatgg aatatttatg cttgttggca
       61 tcaattactt gtcttttcgt ccgaatatca gcgccgcttg ctttaaaata tcattctcca
      121 tttcgagttg cttatttttc tttcttagtg cgatcaattc tgcttgttct ggtgttaagt
      181 tgtctctttc tttgaatgag cccgttgatt gtgcttgctt catccattta tcgaaagaag
      241 aaggcgtaag ctcatactct cgaataattt ctgcgcgagg cttaccagcg agatagagat
      301 cgacgatttg ttgtttgaat tcttttgaat aggttcttcg ttgacgtctt gacatgaaaa
      361 atcctccagt gtgtttttta ttattctaca caccttattt tttctgtcta gtctagtgta
      421 gccgattcag gataaggata agtgtattaa ttgtggaaaa tgtaaaaaag tttgccaaat
      481 gaataaagat tcagttgaga attgcaatca tctggagtgt atcagatgtg gacgatgtaa
      541 aaatgcttgt ccagtggatg caattagtta tggagtaaga aaatagagag gagtagttag
      601 gaaagtgata aaaaatgcta tagcatatat tacaagaaag aaaaatagaa ctttaataat
      661 ttttatcata ttaactatag ttctttcttg cttgtattct tgtttaacaa taatgaaatc
      721 aagcaatgaa atagaaaagg ctttatatga aagttctaat tcttcaatat caataacaaa
      781 aaaagatggt aaatatttta atgttaatca atttaaagat attgaaaaat taaaagaaat
      841 tgaagaaaaa ataattcaat atgatggatt agcaaaatta aaagatgcta aagtagttag
      901 cggagaacaa agaataaata gagaagattt atctgacgaa tttaagaatg ttgtttcact
      961 cgaagctaca aataatacta aaagaaatat tttatttagt agtggagtat ttacaattaa
     1021 agaaggtaaa aatatagaag aaaatgataa gaattcaatt attgttcatg aagaatttgc
     1081 taaacaaaac aatctaaaat taggtgatga agttgatctt gaattactag atattgaaaa
     1141 aagtggaaaa ataaaaagtc ataaatttaa aattataggg atcttttctg gtaaaaaaca
     1201 ggaaacatat acaggattat cttctgattt tagtgaaaat atggtgtttg tagattattc
     1261 aacaagccaa gagatattaa ataattcaga aaataatgaa attgcaaata aaattttaat
     1321 gtattctggt agtgcagaat ctacagactt agccttaaac aaattgaaag agcttaaaat
     1381 tgatgagtca aagtattttg ttgaaaaaga ttctaatgcg tttgaagagt ctttagagtc
     1441 agtgagtgga ataaaatata taataaaagt aatgacttat tctattatgt taggtgggat
     1501 ggttgttctt tcattaatct tgattctatg gttaagggaa agaatttatg aaataggtat
     1561 attcttatct attggaacat ctaagataca aattataatg caatttatat tcgagttaat
     1621 attcatatcg ataccaagta taatatcttc cttattttta ggaaatgtat tactaaaagt
     1681 aattgtagat ggattcatta actcagagga ttcaatgatt tccggtggaa gtttaataaa
     1741 taatagtagt tttatgtcaa atataacaac acttggacaa agttatttaa tattaataag
     1801 tattattgtt ttatcagttg tatttgcttc ttcattaatg ttaatcaaga agcctaaaga
     1861 aatcttatca aaaataagtt aggagaaaat aatggatata ttagaaataa agaatgtagc
     1921 atatagttat gcaaattcta aagaaaaagt tttgtcagga gtaaatcaaa aatttgaact
     1981 tgggaagttt tatgcgatag taggaaagtc aggaacagga aaatctacac ttctttcctt
     2041 acttgctgga cttgataaac ctcaaacagg aaaaatattg tttaagaatg aagatataca
     2101 aaataaagga tatagtaatc atagaaaaaa taatatatct ttagtatttc aaaactataa
     2161 tttaatagat tatttatcac caattgaaaa tattagatta gtaaataaat cagcagatga
     2221 aagtatcttg tttgaactag gtttagataa aaaacaaata aaaagaaatg ttatgaaatt
     2281 atctggtgga cagcaacaaa gagtagctat tgctagagca ttggtatcag atgctccaat
     2341 aatactagct gatgagccta ctggtaatct agacagtgtt actgctggag aaataattaa
     2401 tatattaaag acattagcta aagatagaaa taaatgtgta atagttgtaa cacatagtaa
     2461 ggaagtagca gattctgcgg atatcatttt agaactaagt ggtaaaaagt tgaaaaaagt
     2521 aaataaaatg aatttggagg ttgaataatg ataaaaaatg catttgctta tgtaactaga
     2581 aaaagtttaa aatcattaat tatcatatta gttattttat ctatgtcaac tttaagtctc
     2641 attagtttat ctattaaaga tgctacggac agagcttcaa aagaaacatt tgctaatata
     2701 acaaatagtt tttctatgga gataaataga caagtaaatc caggaacacc tagaggtggg
     2761 ggaaatgtaa aaggtgaaga tattaaaaag atatctcaaa cagatagtat agactcctat
     2821 gtaaaaagaa taaacagtgt tgcagattta gttgattatg atattattga aactcaagag
     2881 actcttgcga atcaatcacc tgaaagagca aagaatttta aaagaacagt tatgttaact
     2941 ggggttaacg actcaacaaa agaaacaaaa tttgtatcag aagcatataa attagtagaa
     3001 ggaaagcatt tagaaaatga agataaaaat aaaatcttaa tgcataaaga tttagctgaa
     3061 aaaaataacc ttaaagttgg agataagata aaaataaaat ctaatttatt tgatgctgat
     3121 aatgagaaag gtgcagatga aacagtagaa gtagaaatta aaggtctatt cgatgggcat
     3181 aatagcggtg tagtaagtgc agcacaagaa ctatacgaaa atacattaat cactgatatt
     3241 catacagctg ctaaagttta tggtaatacc gaagatacag ctgtatacca agatgcaaca
     3301 ttctttgtaa aaggtgataa gaatcttgat agtgtaataa aagatcttgg gaaattagat
     3361 ataaattgga gagaatataa tttgattaaa agttcatcaa attaccctgc attacaacaa
     3421 tctatatcag gtatttactc aatttcaaat aaattatttt ttggctcatt aatatttgca
     3481 ggagttgtag tttcattatt attattctta tggatgaatg ctagaaagaa agaaatagca
     3541 gtattactat cattaggtat atcaaaatta gaaatttttg gtcaattcct aattgagatg
     3601 gtatttatat caatccccgc attcgttggt tcctatttct tagctcaata tacagctgac
     3661 aagcttggga ataatatatt gaataaggtt actggtgata tagctaaaca aatagctaaa
     3721 caatctgcat ctagccaatt aggtggtggg gcagaagctg aaggatttaa caagacatta
     3781 tcaagtttag atatcaatgt attacctaaa ttcatgattt atgtagttat atttatgagt
     3841 ttagtacttc ttgtatcatt gattatttct tcattcaata tattaagaag gaatccaaaa
     3901 gaattattaa ttgacaataa ttaaaatttt cggtgctttt tagcaccaaa aatgagagtg
     3961 taatataaac cgtgtaagta accatcaatc ctaggcgggt ggttgcttca cggtttttat
     4021 ttttctaaaa taagatcaag aggtgattac acgatggcta gaaagaaaag aaatcctgat
     4081 gctgagaagt tagctgaatc cattttgaat acttatcaac ccgaatctgt agaggatatg
     4141 caagatgttt taaaagatgt gtttggtccc ttatttgaaa aaatgcttca aggagaattg
     4201 aataaccatt tagggtatga cgcccattct aaagagccaa agaaacatga caatcgacga
     4261 aacggttatg gcaataagac gcttaaaacg agctttggta aagtaaacat tgatgttccc
     4321 agagaccggg aggcttcttt tgaacctgag ttgattccta atagaaaacg agatgtctcc
     4381 gacatcgcag actgtagaca aaagctgttc caaactcgta actcgagctt ggaacagctt
     4441 tttatctact tcgtttcgaa tttttgaaca aaaaaataaa ggctccttgc ctttccatag
     4501 ccaggtggct aatttcttta aattcattgc agcaaacact agcatcgtat gcgtcgcgac
     4561 tttttccaag cctcgatact tggtccaacg catcccatgc ttttcttttg catccgcaaa
     4621 tattcgttca atcgtttgtt tccgtttttt gtaaattgat ttattcagat ctgttaggcg
     4681 aagatgttcc acttcatcca tcagatctcc ccaaacatgt ctcgtgatga ctcgttgatg
     4741 atttttactt ttggtacatt ggtttagcag aggacagttc acacattctt ttggattgga
     4801 tttgtattct cggtaccttt ctcgagtaac cgttgaaaaa gcgagcattt gatcttgcgg
     4861 acaaatataa caatcaaaat actcgtcata gagaaactca ttttttgata atagcccctt
     4921 tttccctttg ggccttgaat aaggcaaaca aggtcgaagg ttcaaatgag tcaaaaagtg
     4981 aacaaactta ggtgtcttat acccagcatc agcgacaaca caattgattt caggaaaa
//
LOCUS       DAZABD010000117         4974 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000117 DAZABD010000000
VERSION     DAZABD010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4974
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.122"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..241)
                     /locus_tag="ACHALG_002757"
     CDS             complement(<1..241)
                     /locus_tag="ACHALG_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFN2588063.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEIIFPAEEKEYI"
     gene            513..857
                     /locus_tag="ACHALG_002758"
     CDS             513..857
                     /locus_tag="ACHALG_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFN2588064.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            858..1277
                     /locus_tag="ACHALG_002759"
     CDS             858..1277
                     /locus_tag="ACHALG_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFN2588065.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVIFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFGHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1369..1719)
                     /locus_tag="ACHALG_002760"
     CDS             complement(1369..1719)
                     /locus_tag="ACHALG_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2588066.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1685..3016)
                     /locus_tag="ACHALG_002761"
     CDS             complement(1685..3016)
                     /locus_tag="ACHALG_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HFN2588067.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            3272..3838
                     /locus_tag="ACHALG_002762"
     CDS             3272..3838
                     /locus_tag="ACHALG_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN2588068.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3983..4070
                     /locus_tag="ACHALG_002763"
     tRNA            3983..4070
                     /locus_tag="ACHALG_002763"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4015..4017,aa:Ser,seq:cga)
     gene            4404..>4974
                     /locus_tag="ACHALG_002764"
     CDS             4404..>4974
                     /locus_tag="ACHALG_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HFN2588069.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAARVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNG"
BASE COUNT         1773 a          839 c          954 g         1408 t
ORIGIN      
        1 caatgtattc tttttcctca gcaggaaaaa tgatttcggt tctcccacaa cgtcgcttat
       61 tttctttgta ttgttcaaag tattcaagtg ctgttccacc acatttgaga aagttataga
      121 cattgtaaat tgtttggcgt ccacgtttaa gctgcttcgc aacgatagca accggagttt
      181 cttgatgaaa atatgcttct atcattacaa gctcgtttgg tgtaagatgg gtataagtca
      241 tttatgttca ctctccttgt atgctttagc gggtattaca atttgagtgt aacataaatg
      301 gcttttttat ttgtctcgct taattataca aacggcgtaa aataaaaaaa gcgttagcaa
      361 ctatgcttaa aaagcatatc gactgaataa actaagcatt ggacaggctg aaagactaca
      421 tttaaaatga aatgcgaaga aacaatagaa aataaagttg ccttagaacc atagttgaat
      481 cacctaacag tcgtgaaggg agaataagag aaatggcaga aaaacaaacc gcaggacgag
      541 atcaattagg agattttgca ccaaaatttg cagaattgaa tgatgatgtt ttatttggac
      601 aagtatggtc cagagaagaa ttatctgctc atcagcgtag tatcatcacc atatctgcat
      661 taatttcagg aggaaatttt gaacaattgc cagctcatct gaagattggc aagcaaaatg
      721 gcattactag tgaagaaata gcagaaatca ttacccattt agctttttat gtaggatggc
      781 caaaagcttg gtcagctttt agtttagcaa aagaaatcta tttagaagaa aatagcgaag
      841 ataagaaaga ggcgtaaatg acgacaaaac acgaagaaat acacaatggc gtgatttttc
      901 cagcaggtga aaaaaacgaa gcttacgctc aagtatttac cgggcaaagc tatctgaata
      961 tgttagtgaa tgatcccgaa gtttctgttg gtgtaggtaa tgtaattttt gaaccaggtt
     1021 gtcgcaatca ctggcatgtc cataaagacg gctatcaaat cttacttgtg accggaggag
     1081 aaggctggta tcaggaagaa ggaaagccag caagacgatt aatggctgga gatgtcgtcg
     1141 taacgcacaa agggattaaa cattggcatg gtgcgacaaa agacagttgg tttggacatt
     1201 tggcgatcac tgctggtaat tcagagtttt tagaacctgt aacagatgaa gtgtatgaag
     1261 gattggaaaa tcaataatgt caagcttgaa cagctcctga aagtagattt taatcgaact
     1321 ttcaggagct gcttttttga ttcagtcttt tataaataga aaatagggtt aataatagga
     1381 atgagaagca cgtcgaaaga tcttaatatt gataatcggc tgggcataca tgatttccac
     1441 gtcatcaatc aaactaccta acagatgata atcttcccga tcatgtcggg gattacctaa
     1501 tagttcttga tagtagattt ccatactagg gtcccgagga ttgttcagca aatcagcaag
     1561 tacatctaaa tcttctggct gagtttctgc cgtaccctgt atctctatga aaagcccttc
     1621 ctcagcaaat tctactccaa tacgtatttc agttatttca tgggaatata aataagtaag
     1681 aagctcatcg atgattcttt ttgataattc tttttccaag tgtctttttt tcctttctga
     1741 cagcggtaaa gttatctagt atcggtacaa ggatacctgc aacgaaacca gttgaaaaac
     1801 cactgttata tagatttagt cctccgtgca aagtgctgac gttatggaca agagcaatat
     1861 gtagtacacc agctattaca ccgtaaaaac tgccgtaaaa accgcttatg ggcgcgagac
     1921 ctgtcccaaa cacagctgca acaagcatgc cagtactggt gatctcatga aaagttccaa
     1981 acaaagaagc aataaatatt ccgactagta tcggaaaact gttttttaaa tgacaaccaa
     2041 aagcactgaa acccaccgca gataaaatag caccgatgac aggtccattt aactgaccgc
     2101 caaccagcaa aacataagaa gacagcatca gaccaactaa gcctatgttg atcaaagtag
     2161 caccaatacc agaattagca atgaaatcag ttgtcaattt tccagatgaa tcgaaaattt
     2221 ctcgaatccc caaaagccga aaggaattat aatagaaacc aattccgact gtcagcgaaa
     2281 aaaagccaaa aataatccaa atgagaggaa agtgatattc attgaaaacc aatgtatttt
     2341 cgacgattga atagctgaat aacctcaaaa cagcagtaaa agtcatcgca atcaacccag
     2401 aggtgaatcc aatattataa agtgtgaatc cacgatggaa aactaaagtt tgagcagcta
     2461 agggcggaag aagtagacca attgctatgc cagctaaatt ccccaataaa atacctatca
     2521 gtaaaggaaa acgcatacca aaagtgatat aactaacgac cggtgataat gcgctaccaa
     2581 acagtccgat catgatgtac tgagaaaaag gtttattgac aaatacagcg tatagataca
     2641 cacctaaaat gattgagata gaattataaa ggtttttgcc aaaaaaagag aaaccggtaa
     2701 ctgttagaat cgaagcaaat aagggaccgt ttaaaatcac cttgtgccga taagctaaaa
     2761 gcacagaaag taaggtcaaa atccctacat ttatgaatgc gcttccgaaa ccgcctaaag
     2821 caaaataatc tgtcaaaaga ttgcttggcg aggtaagaat agttagcaat ccactccaat
     2881 tatcagtagg agtattagaa agcaagccaa taaaaagaaa aaaaacacct gtaaaaccaa
     2941 gataaaaata ctttcttttc tgtgaaacga ttcgtttttg aggatgaata taaataacgt
     3001 cagtttcgtt ttccattaca caccttcttt ttcattttct ttaactataa cttgaaatga
     3061 aagatttgga caactatcat ttaggtcaaa actaaaaatg taagggaacc cattgaattt
     3121 aaccaaattt ttttaatttt gcaaaaaaaa gttaaagaag actgaaaaaa aatttaaaat
     3181 ataacgtttt gagtaaaatg tttgcaaaaa atgctaaaaa tagggtacaa taaaacaaca
     3241 tatactgcag taaagaagtg ggggagtttt aatgagaaga aaagtctata ccaaggatca
     3301 aattctaaaa gctgcttatg atgttgttgc aaaggaaggg tttaaaggat ttactgcgag
     3361 aaatattgct aaaaagatgg ggatttcgac acagccgatt tatctggaat tcaaaaacat
     3421 ggaagattta aaaaatacgt tgctggattc tatctttgaa caactaaaag aagatgtttt
     3481 ccctattgaa cgtactggtg ataaaatcat tgatctcggt ttgaactata tctactttgc
     3541 aaaaaagcat catagtcttt acatggcatt gtacattgat gaacatgggg acggaaagaa
     3601 aatgcatgat ttttcataca attatttccg tgaaatagtt aaagaagatg aaagatacaa
     3661 tcaattatcg gatgaatatg tcgatgctct gcatactggt acatgggtag cagtgactgg
     3721 cttagcgtca ttgatgtcat caaacatcat gcatccaaca gaagaacaaa tcattgcact
     3781 gatcaaacgg acaattgact cgattttagc attagataat cctgaaataa tcagatagtg
     3841 ttctatcagt atgaatttga tcggtaagtc cgttggctgc aaactgtcaa tggacttttt
     3901 tgctaaaaaa agcgatttct gttatttatc tattagaaat aggcttgaca tttcgtgaaa
     3961 aaagtttaaa aatagtactt acggaaggtt ggcagagtgg taatgcaccg gactcgaaat
     4021 ccggcgaacc ggctaatacc ggcgcgcagg ttcaaatcct gtaccttcct tatatcaacg
     4081 attagaggtt gtgaagaagc tgtcctgcat caagtaataa gaacggccaa acaaaaatag
     4141 cttttcctat tttttcgttt gattgggctt attaccgcag atgcaagctt ttgaacaccg
     4201 tttgttcgga ataaggggct gtgacaagac ttttgtcaca gccccttatt gttgtacttt
     4261 taacctacaa tattttttga ttccccctga gtactatttt ttataaatgc tagtaaatca
     4321 atcttctttg gccttttttt gataaaaaaa atactagatg taagataaaa gtgtaaaaac
     4381 gatttgtttt aggaggggaa acgatgtgta ccgcaatcac ttatgcaacg aaagaccatt
     4441 attttggaag gaattttgat tatgagatgt cttataatga agtggtcact attactccaa
     4501 gaaattatcg tttcgatttt agaaaggtaa aaaatttaga taaacattat gcaatgatag
     4561 gaatagcagc tagggtatcc aactatccac tctattatga ggctacaaat gaaaaagggt
     4621 tgagtatggc aggactgaat tttcctggca atgctgatta taaagaatta caagaaggaa
     4681 aagataacgt tgcaccattt gagtttattc catggatact agggcaatgc tcaaccatag
     4741 acgaagctaa agaattattg gctactatca atctagtcaa tatcgatttt agtgaaaaac
     4801 tacccttgtc acctttacac tggttattag ctgataaaga aaaatcgata gtaattgaaa
     4861 gtatgaaaga tggacttcat ttatatgata atcctgtcgg tgtgttgaca aataatccgc
     4921 catttgatta tcaattattt aatctgaaca attatcgctc gctatcaaat ggaa
//
LOCUS       DAZABD010000118         4964 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000118 DAZABD010000000
VERSION     DAZABD010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4964
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.123"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>4964
                     /locus_tag="ACHALG_002765"
     CDS             <1..>4964
                     /locus_tag="ACHALG_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033926 -
                     endo-alpha-N-acetylgalactosaminidase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="endo-alpha-N-acetylgalactosaminidase family
                     protein"
                     /protein_id="HFN2588070.1"
                     /translation="ENTNETSAVLDNENNKEWVSVSQVPGTVVVNEVEGIRYNTLSST
                     LENDNGNKMALFEKDGLHVDDAGNATVSLSFVEQSKTGEGRFGVLLKQSDNQNYIMVG
                     YDRAGWFWEYKSAVDSAWMTTKRSVKAPTKGSKNELMISLKHDGQLNASVNGENLFDT
                     YNVPQNVIDALKDNKTIALKLGTFGNEITKIDIKTDNQSNVATSNTVKDKEVGITVDD
                     SNIIYDTIKTDQIEAVIDVSFPRVKEYHVDNHILPGQVQPLNIVKINGIDVTPITSFE
                     KINDSAALYKMKLKNLEHFINAEIEVKLQVVDNELHFDVVKFVNYNKIVAGETIDDTR
                     KLIETIEFPDNYLVSVSSKDDKAAFDGARMSTNTHKNGDIHINVTNPMEDIPTTGFMY
                     GFVSNNKLAAGVWSNSQYSYGGGDNDYTRLTIGKQTINGDNYLGILSSPFIYQKHYKG
                     QVYDERTIELPSAKVVITNDLNEDEVVDWQDGAIAYRSIMNSAKGSEHVPDLVAYRIA
                     MNFGSQAQNPFLTTLDGIKKISLHTDGLGQSILLKGYGSEGHDSGHLNYADIGKKIGG
                     SEDFKKLIELSQKYGARLGIHVNASETYPESKYFNPERLKKNSDGTYSYGWNWLDQGI
                     NIDAAYDLGHGRFKRFQDLKEVLGEGLDFIYVDVWGNGQSGDNNAWATHQLAKEINDN
                     GWRVAFEWGYAGEYDSTFQHWAADLTYGGYSLKGINSNIVRFIRNHEKDSWIGDYISY
                     GGAANNPLLGGYDMKDFEGWQGRSDYAGYIKTLFKSNLPTKFIQHFQVTNWKNGTPVF
                     MKDNGESYEWIPEMEIKLKDQENRRLVISRKSNNVNDAGYRERVMTLDGRKIYDGSSY
                     LIPWDWASDGSKLSKNDQKLYYYNTTTGSTTWELPSDWLVNKVYLYKLTDLGKTDETI
                     LDVVNGRITINVNAETPYVIYRTPHNNEEVIWSEGMHIVDQGFNSGTLAHWALTGEEN
                     TAVEIVRSQGDNPMLRIMDNSSKVSLTQKLSGLKPNTSYAVYVGVDNRSDSSAEISID
                     TGEKIISNSTGRSIALNFVKAYAHNNLKNNATIDNLSFFQNMYIYFTTGENVDNVILT
                     LSRDAGEGATYFDDIRIFENNSIMFNGTHDSSKGIFEQDFENVPQGIYPFVIGGVEGV
                     EDNRTHLSEKNSPYTQRGWNDKKISDVIDGNWSLKTNGLTGRNKLLYQTVPHNFRFEP
                     GKVYRVSFDYEAGSDDTYAFVVGNGEYQNNPSELKMYSLKNTWTDSEKAKKVSYYVTG
                     SESGNTWIGIFSITNSGDTHGVEGNAANFEGYLDFVMDNLVIEEIKLTPITKLELAIE
                     EFLPFDDSLFTKETSDRYKEALGKLVTADPYELTLSDIDKLIEDIRNKKEELILKKLY
                     IDKGDIHSMEANEQPGESIELAFDGDTNTIWHSKYNETAINVPAKIDLKVPTSIKGFV
                     YVPRLSGKNGRVKSGILEITDVLGKIFTFDFSEWKNDSNEKSLMFDQPIMAKSFNFIA
                     KESYGDTPNKFVSASELKFLLPVEEIVEPDRTLFMDTLSLVGKKLGKDSKQIDSIIHE
                     FNLYDKHNLITEKVLIDLVNKWNLLKPLDYNDDNYEPKPQKNEISLESNDKVMVKKQK
                     KDNLEKDSDKKSTSKSNDSNDNNVVGIIRQDDPLININNKSRYN"
BASE COUNT         1836 a          582 c          995 g         1551 t
ORIGIN      
        1 cagaaaatac taatgaaact agtgctgttt tagataatga aaataataag gaatgggttt
       61 ctgtttcgca agttcctggc acagtagtcg ttaatgaagt ggaaggaatt cgctacaata
      121 cattatcttc tactcttgaa aacgataatg gaaataaaat ggctttgttt gaaaaagatg
      181 ggttacatgt agacgatgcg ggaaatgcta cagtaagttt gtcttttgta gagcaatcta
      241 aaacaggaga aggacgattt ggagttctat taaaacaaag tgataatcaa aattatataa
      301 tggttggata tgatagggca ggatggtttt gggaatataa atcagcagtt gattctgctt
      361 ggatgacaac taaacgttct gtaaaagcgc caacgaaggg atcaaaaaac gaattaatga
      421 ttagtcttaa acacgatggt cagttaaatg caagtgttaa tggcgaaaat ctttttgata
      481 cttacaatgt accccaaaat gtgatagatg ctttaaaaga taataaaaca attgctttaa
      541 agttgggcac atttggaaat gaaataacaa aaatagatat aaaaacagat aatcaatcta
      601 atgttgctac ttcaaataca gttaaggata aggaagttgg aataactgta gatgattcta
      661 atatcatcta tgatacgata aaaacagatc aaatagaagc agttattgat gtttcatttc
      721 ctagagtaaa agagtatcat gttgataatc atattcttcc gggacaagta caacctctta
      781 atattgtcaa aatcaacgga attgatgtga cacctataac ttcttttgaa aaaatcaatg
      841 attctgcagc attgtataag atgaaattaa aaaatttgga acattttatc aatgcagaaa
      901 tagaagttaa acttcaagtt gtagataatg aactgcattt tgatgttgtt aaatttgtca
      961 attataataa aattgttgcg ggggaaacaa tagatgatac acgaaaatta attgaaacaa
     1021 ttgaatttcc tgataattat ctggtttctg tttctagtaa agatgataaa gctgcttttg
     1081 atggtgcaag aatgtctacc aatactcata aaaatggaga tattcatatt aatgttacaa
     1141 atccaatgga ggatattccg acaacaggat ttatgtatgg atttgtatcg aataataaat
     1201 tagcagcagg tgtttggtcc aactctcagt atagctatgg tggaggagat aatgattata
     1261 ctcgtttaac aattggtaaa caaacaatta atggtgataa ttatcttggt atattaagtt
     1321 ccccgtttat ttatcaaaaa cattataaag gccaagttta tgatgagcgt acaattgagt
     1381 tacctagcgc taaagttgtc attacaaatg atttaaatga ggatgaagta gtagattggc
     1441 aagatggtgc tattgcctac agatcaatta tgaattctgc aaaaggttct gagcatgttc
     1501 cagatttagt tgcttatcgt attgctatga actttggatc acaagctcaa aatccttttt
     1561 tgactactct tgatggtatt aagaaaatta gtttgcatac tgatggtctt ggacagtcaa
     1621 ttttattaaa aggatatgga agtgaagggc atgattcagg acacttgaat tatgctgata
     1681 tcggaaaaaa aataggaggt tcagaagatt ttaaaaaact tatagagtta tctcaaaaat
     1741 atggagcacg cttaggaatt catgttaatg catctgaaac atacccagaa tctaagtatt
     1801 ttaatccaga gcgtttgaaa aaaaatagtg atggaacata tagttatggt tggaattggt
     1861 tagatcaagg aattaacatt gatgctgcgt atgatttagg tcatggtaga tttaagagat
     1921 ttcaagattt aaaagaggtt ctcggagaag gacttgattt tatatacgta gatgtatggg
     1981 gaaacggtca atcaggtgat aataatgcgt gggctactca tcaattagca aaagaaataa
     2041 atgacaatgg ttggagagta gcttttgagt ggggctatgc cggagaatat gattctacat
     2101 ttcagcattg ggcagcggat ttaacttatg gtggctactc attaaaagga attaattcta
     2161 atattgtacg tttcattcgt aatcatgaaa aagattcatg gattggagat tatatttctt
     2221 atggtggagc agcaaataat ccacttcttg gcggatacga tatgaaagat tttgaaggat
     2281 ggcaagggcg tagcgattat gcagggtata ttaaaacgtt atttaagagt aatcttccaa
     2341 caaaatttat tcaacatttt caagtgacga attggaaaaa tggaacacct gtatttatga
     2401 aagataatgg tgaaagttac gaatggattc ctgaaatgga aataaagcta aaagatcaag
     2461 aaaatagaag gctagttatt tctagaaagt ctaataatgt aaatgatgca ggttatagag
     2521 aacgtgtgat gacattagat gggcgtaaaa tttatgatgg ttcttcatat cttattccat
     2581 gggattgggc aagtgatggt agtaaattat ctaagaatga tcaaaaactt tattattata
     2641 ataccacaac aggtagcact acatgggaat tgccaagtga ttggttggta aataaagttt
     2701 atctgtataa attaacagat cttggtaaaa ccgatgaaac aattttagat gttgttaatg
     2761 gtaggattac gattaatgta aatgcagaga ccccatatgt gatttatcgc acaccacata
     2821 ataatgaaga ggtaatttgg tcggaaggaa tgcatatagt tgatcaggga tttaatagtg
     2881 gtacgttggc acattgggca ttaactggtg aagaaaatac ggcggttgag attgttcgtt
     2941 cgcaaggtga taatccaatg ttaagaatta tggataatag ttcaaaagta agtttgactc
     3001 aaaaattaag tggattgaag ccaaatacta gttatgcagt ttatgttgga gtagataacc
     3061 gtagtgattc aagtgcagaa atatctattg atacaggaga gaagataatt agtaatagca
     3121 caggtcgctc cattgctttg aattttgtta aagcttacgc tcataataat ttaaaaaata
     3181 atgcaacaat tgacaatctt agttttttcc aaaatatgta tatttatttt actacagggg
     3241 aaaatgtaga taatgtaata ttaacacttt caagagatgc aggagaaggt gcaacttatt
     3301 ttgatgatat tcgaattttt gaaaataatt ctataatgtt taatggaaca cacgatagta
     3361 gtaaaggaat atttgaacag gattttgaaa atgttccaca aggaatttat ccatttgtaa
     3421 taggtggagt cgaaggggta gaggataatc gaacacatct ttcggaaaag aattctcctt
     3481 atacccaacg aggatggaat gacaaaaaaa tatccgatgt tattgatggt aactggtcac
     3541 ttaagacaaa tgggttaact ggtcgtaata aacttctcta tcaaactgtc ccacataatt
     3601 tccggtttga acctgggaaa gtttatcgag taagttttga ttatgaagct ggttctgatg
     3661 atacgtatgc gtttgttgtt ggcaatggag aatatcagaa taatccttct gagttaaaga
     3721 tgtattctct taaaaatact tggacggaca gtgaaaaagc aaaaaaagta agttattatg
     3781 taacaggtag tgaaagtggg aatacatgga ttggtatatt ttctataaca aattctggtg
     3841 atacacacgg agttgaagga aatgcagcta attttgaggg atatcttgat tttgtaatgg
     3901 ataatcttgt tatagaagaa atcaaattga cacctattac caaattagaa ttagctattg
     3961 aagaattttt accatttgat gattctttat ttacaaaaga aacttcggat aggtataaag
     4021 aagctttagg taaacttgta actgctgatc catatgagct gactttatct gacattgata
     4081 agctaataga ggatataaga aataaaaaag aagaattaat tcttaaaaag ctttatatag
     4141 ataaaggtga tattcatagt atggaagcaa atgaacagcc tggagagtcg atcgagctag
     4201 cttttgatgg tgatactaat acaatttggc atagtaaata taatgagaca gcaattaatg
     4261 tgcctgcaaa aattgatttg aaagttccaa cgagtattaa agggtttgta tatgttcctc
     4321 gtttatcagg gaaaaatggg cgtgttaaat caggtatatt agaaataaca gatgtattgg
     4381 ggaaaatctt tacgtttgat ttttctgaat ggaaaaatga tagtaatgaa aaaagtttaa
     4441 tgtttgatca accaataatg gctaaatcat ttaattttat agcaaaagag tcttatggtg
     4501 ataccccaaa taaatttgtt tctgcctcag aattgaaatt tttattaccg gtggaagaaa
     4561 ttgttgaacc cgatagaacg ctttttatgg atactctatc attggttgga aaaaaattgg
     4621 gaaaagatag taaacaaatt gattctatca ttcatgagtt taatctttat gacaaacaca
     4681 atttgataac tgaaaaagta ttaatagatt tagtaaacaa atggaatttg cttaaaccat
     4741 tagattataa tgatgataat tatgaaccta aacctcaaaa aaatgaaatt tcattggaaa
     4801 gtaatgataa agtgatggtt aagaaacaaa agaaagataa tcttgaaaag gatagtgata
     4861 agaaaagtac cagtaaatct aatgattcta atgataataa tgtagtagga atcataagac
     4921 aagatgatcc attaataaac ataaacaata aaagcagata taat
//
LOCUS       DAZABD010000119         4867 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000119 DAZABD010000000
VERSION     DAZABD010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4867
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.124"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            500..1660
                     /locus_tag="ACHALG_002766"
     CDS             500..1660
                     /locus_tag="ACHALG_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFN2588071.1"
                     /translation="MKITEKNVNPKFETTESNLKKFDLTVTLSNQLVDSIVEMDAMPQ
                     KIYLYAIGHLDPKNPPFNNTVKVSKKALFALMSSSSSSSKHSRLKQVIEKLHQQSVIK
                     IQRTKPNGKTEYKVISPLEITEFNDFSDAVSIKFSSSIMPLLTNLENHFTQYTFDQVV
                     NLTSKYAISFFQLFTKDFNLYEANRDNLTWSTKSLDRLKNPVFSIADLRKKFSIEKKY
                     KAFRDFERRVIKEPIAQLNKYTDFKIDYELIKKGHTYTNIKFLLTRTMSVPVPYKQEQ
                     RDPAYLKGAEDKTEKMNQSYLKAMNSKYTKLLIKNFLISSADLCDLKLMSRLQNKVYP
                     LYDRLSKDRGFESVVNHLSYVATHKEDYTIKNTVKYLEKSITSYLPYVNLYD"
     gene            1695..2462
                     /locus_tag="ACHALG_002767"
     CDS             1695..2462
                     /locus_tag="ACHALG_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFN2588072.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPTKLKKT
                     GVVDLPVKAIINYMDVDQNLETELKTIVNTLQRQAVYEISNLDKKYKTYTTVSAIDRI
                     HWDLEEHSVSFSFSEDVIPLIVSIKKKYPFFVFEDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LNIDFAKVRKGRNYNFAVQFNISSEFDEKTYREKKLG"
     gene            2654..3052
                     /locus_tag="ACHALG_002768"
     CDS             2654..3052
                     /locus_tag="ACHALG_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFN2588073.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
     gene            complement(3359..3733)
                     /locus_tag="ACHALG_002769"
     CDS             complement(3359..3733)
                     /locus_tag="ACHALG_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2588074.1"
                     /translation="MKQTQTETVNDTLADQLAGYYKLFEMGKAQRLIHYLWKYKILFL
                     FWESTLTFSVLFYKSDFYNQHLVLGSFLLIYTILMLNLYTYITLEEHLVISDAGIERN
                     IKRVSFKITLNKPTDKKLSEAF"
     gene            complement(3755..4105)
                     /locus_tag="ACHALG_002770"
     CDS             complement(3755..4105)
                     /locus_tag="ACHALG_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2588075.1"
                     /translation="MKATHLNYFSRTLINNLLKDTTGISVSTKHFANDLIIFEDNNLR
                     IEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKKAKLLFEFQT
                     DYHNELLAQSEILD"
     gene            complement(4122..4517)
                     /locus_tag="ACHALG_002771"
     CDS             complement(4122..4517)
                     /locus_tag="ACHALG_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFN2588076.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEELLEK"
     gene            complement(4584..4769)
                     /locus_tag="ACHALG_002772"
     CDS             complement(4584..4769)
                     /locus_tag="ACHALG_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFN2588077.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
BASE COUNT         1681 a          719 c          829 g         1638 t
ORIGIN      
        1 tataatatat attttaatct ttaaaacttt agcaacctcc aaaaaggctt tatatcaatc
       61 ttaaatagat tttagggtgg acataaagcg atgcgaacat ggacaaaaag cgatgtgaat
      121 gtggacaaaa agcgatgcaa acgtggacaa aaagcgatgt ggtattccga atatatccaa
      181 aaaaagaaaa agtatgtgaa tttatttcta aaaactagaa aaatatttgt tttttaattt
      241 tggtaatttt tataatttcg tattttattg aaaaggattt ttattttgaa aatgttctaa
      301 aatagcgatt tctgctgtat tttcaatgaa atttgattgg aaaaaataaa atgtgtttta
      361 ctgacattga gttatgttat aaatttacga tacatttctt attgtaacgt atcgaaaaca
      421 ggtctatact tattcaatgg aatcgtacca gttccaagtt tttaagctcg taccaagctt
      481 agaaagaaga ggcaatcgta tgaaaataac agaaaagaat gtaaacccga aatttgaaac
      541 taccgaaagc aatctaaaaa agtttgattt aactgttact ttatcgaatc aacttgtaga
      601 tagcattgtt gaaatggatg ctatgccaca aaaaatttat ctttacgcta taggacatct
      661 tgaccctaaa aatccgcctt ttaataatac cgtcaaagta tctaaaaagg ctttatttgc
      721 gttgatgtct tctagttcat cttcttcaaa acattcaaga ttaaaacaag ttattgaaaa
      781 attgcatcag caatctgtca ttaaaattca aagaactaaa cctaacggta agactgaata
      841 caaagtcatc tcaccgctag aaattactga attcaatgat ttttctgatg cagtatctat
      901 taaatttagt agttcaatta tgcctttatt aacgaactta gaaaatcatt ttacgcaata
      961 tacatttgat caagtagtta acttgacaag caagtatgcg atcagttttt ttcaattatt
     1021 cactaaagat tttaatctgt atgaagcaaa tagagataac ttaacatgga gtacaaaatc
     1081 acttgataga ttaaaaaatc ctgttttctc cattgcagat ttacgaaaga aatttagtat
     1141 tgagaaaaaa tataaagctt ttcgagactt tgaaagacgt gtaattaaag aaccgattgc
     1201 ccaactgaat aaatacactg attttaaaat tgactatgag cttatcaaga agggtcatac
     1261 ttatacaaac ataaagttct tgcttacacg taccatgtca gttccggtac cttataagca
     1321 agaacaaaga gaccctgctt atttaaaagg cgcagaagat aaaacagaga aaatgaatca
     1381 atcgtacttg aaagctatga atagcaaata cactaaatta ctaatcaaaa actttttgat
     1441 cagttctgcc gacttatgcg atcttaaact tatgagccga cttcagaata aagtttatcc
     1501 gctttatgat cgtttatcca aagatagagg atttgaatca gttgtcaatc acctatctta
     1561 cgttgctact cataaggagg attacacaat aaaaaatact gttaaatatc tcgaaaaatc
     1621 aattacgagt tatttgcctt atgtgaacct ttatgattaa caactgaaaa aatgcgtgaa
     1681 ggaaggaaaa acttttgaaa aaagatgaac tgacaaagta caatgaggag ttcttagcaa
     1741 caattgacat gaaaatggat ttaaagtcgt atgaacttta tcaattgctg ttcagttata
     1801 tctcacccac aaagctaaag aaaacaggcg tagtagattt acctgttaaa gcgattatta
     1861 attacatgga tgttgatcaa aatcttgaaa cggaactaaa aaccatagtt aacactttgc
     1921 aaagacaagc agtttatgaa atttcgaatc tagacaaaaa atataagacg tatactactg
     1981 tatctgctat tgatcggatt cattgggatt tagaagagca tagtgtatct ttcagttttt
     2041 cagaagatgt aattccctta attgtctcaa tcaaaaagaa gtatccgttc tttgtttttg
     2101 aagacttagc atcttttcaa aaaaaatata gcatcgtgtt atacaaatat ttggcgatgt
     2161 attataaaaa atatcttatc gccaaaaata aaggagattt tgagaaagct gaaatatata
     2221 agaatcccct gattgaaata gttaaaattc gtgagattac gaaaacagaa aatgagtata
     2281 aaacaatatc gaattttgat agacgtgttt taagggaatc agttgaggaa attaatgaaa
     2341 aatcaatgct gaatattgat tttgctaaag ttagaaaagg cagaaattac aattttgctg
     2401 tccagtttaa tatttctagt gagtttgatg aaaaaactta tagagaaaaa aaattaggtt
     2461 aatgcaaaat atatcttact cgtttgaaga tgcttcagac gtatgctaag atatatagtg
     2521 ccttgggagc aaggtattaa tctcctttag aggttaatta gcttgtgatc gcctatcaat
     2581 ttttttggta ggcgattctt ttttttgaaa caaaaaagca aaaattattt tttaattaga
     2641 gaggtgcaat gcgatgaaat actttttttg tgcagtattc gaatggaaag gaaatgaata
     2701 tgcagttaaa ttcccagatt ttccagactt atatacacat ggtgaaactt tagaagatgc
     2761 ttttgagatg gcgcaagaag tattggaagg atacttggtg cttttagagt tgcaagaaaa
     2821 agaaattcca tcgcctacgc ctgttcaaga gttaaatgca agaatcaagc gatctgagaa
     2881 tgttaattta tttgtcgtaa aagctaatac gaaagttgct aaagctcgaa acaagcaaaa
     2941 ttatgatcga aaatcagtga ctgtggaaca ctatttaaat gttttagcgg ctgaagagaa
     3001 tattaatctg tcgaaagctt tgaatgattt gttgagagaa aagttgattt gatgtctctc
     3061 agatgatcat attaagcaaa aacagcttgt tttaggacgt atggatttaa gaaagacaaa
     3121 ttatcaaaga atatattaaa agctctcaga agccttctga gagcttttaa attgctatgt
     3181 attgtactgc atctgctcaa attgattttc ttttcatgct taccatgtat ctaattttcc
     3241 atcaagtaat ttttagatat gtattgagta ataaatagca aatttgcttg atataagctc
     3301 gaattaggtg ctttgtaatg tgttaggata aaattataca agaaaatgtc aaaagctctc
     3361 agaacgcttc tgagagcttt ttatcggtag gcttgtttaa cgtgatcttg aaggataccc
     3421 ttttgatgtt tctctcgatc cctgcatcgc taattactaa gtgttcttca agtgtgatgt
     3481 aggtatataa attaagcatc aaaatggtgt agataagcaa aaaactccct aaaactaagt
     3541 gttgattata aaaatctgac ttataaaaaa gaactgagaa tgttagtgtt gattcccaaa
     3601 aaagaaacaa aattttgtat ttccaaagat agtggatcaa gcgctgtgct ttacccatct
     3661 caaataattt ataataacct gctaattgat cagcaagcgt atcgttaacg gtttctgttt
     3721 gtgtttgttt catctttctc ttctcctgtt tcaattaatc taaaatttca gattgagcaa
     3781 gcaactcatt gtggtagtcg gtttgaaatt caaataacaa ttttgccttc ttaacgaggt
     3841 ttaaagcgtt cgttagtgat tcacttaatg cttcaacatc tttaactgat tttgcatgtt
     3901 tcattttttc agtgtgcgta tagcagaatt caggtattga gttaggggtt gcctgttcaa
     3961 taaacatctc aattcgcaag ttattatcct caaaaataat tagatcattg gcaaagtgtt
     4021 tggtactaac gctaatgcca gttgtatctt ttaatagatt attgattaac gttcgagaga
     4081 aatagtttaa gtgtgtagct ttcataggtt cggtctcctt tttacttttc aagcaactct
     4141 tcttctagtg ctttttgaag tagttgagag aagtttatgt ttttctcctt ccctaaaaga
     4201 actaaccatt ccggaacact aacatttttt ctaattgttt ttgtatttaa gcttcttcgg
     4261 aaaagtttaa tgtttagttg gatgattact accacttggt tggctttcaa agttaattct
     4321 tccactgctg atggtttagg aaattctgca tctggtacac tatatagcgc taatcctaaa
     4381 gcatccttag cgtttaaata tgcttcttca agactatttc caaaggtaga tatttccggt
     4441 aagtcaggga aaacgatgtt ataagcttct ccttctttct cgaagattgc cggataaaaa
     4501 atattttttg tcttcatttc ctcgctcctt ttctagtgta agagaggata gtatgcctaa
     4561 taccaaatag gaaggcaagg ctgttaaagc cctgcttgct tccagatatt agcttctgtt
     4621 cctagtggta catcaccatt gtggatcgga acaacgaccg ttcttttagt cttggaatta
     4681 taaaattttg catgactgcc agtttgtcca gtctttttga agccgttaga tttgagaatt
     4741 tttacaattt ctcttgctgt cttaggcata gctaccctcc ctttacaaaa tctattatac
     4801 acattgttgt gtatggaatc aatagaaaaa gaggatgttt taacccgtgg tgctagttaa
     4861 ttttttc
//
LOCUS       DAZABD010000120         4808 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000120 DAZABD010000000
VERSION     DAZABD010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4808
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.125"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            90..1511
                     /gene="gndA"
                     /locus_tag="ACHALG_002773"
     CDS             90..1511
                     /gene="gndA"
                     /locus_tag="ACHALG_002773"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFN2588078.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
     gene            1704..2390
                     /locus_tag="ACHALG_002774"
     CDS             1704..2390
                     /locus_tag="ACHALG_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFN2588079.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            2387..3892
                     /locus_tag="ACHALG_002775"
     CDS             2387..3892
                     /locus_tag="ACHALG_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFN2588080.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            4579..>4808
                     /locus_tag="ACHALG_002776"
     rRNA            4579..>4808
                     /locus_tag="ACHALG_002776"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         1669 a          806 c         1006 g         1327 t
ORIGIN      
        1 aaatatgttg ttctttagtt tgcaatgtag ccgtgttaag ctataatgta agagattagg
       61 gagaaatccc gtatgtagga ggagttcata tgtcaaaaca acaaatcggc gttgtcggta
      121 tggccgttat gggtaaaaat ctagccttga acatagaaag ccgcggctat tcagtggctt
      181 tattcaatcg tacaggggct aaaacaaccg ctgtagtaga agaacaccca gataaaaatt
      241 tcaaagcaac atatacaatc gaagaatttg tagaatctat cgagaaacca cgtcggatct
      301 tgctaatggt caaagcagga ccggcaacag atgcaacaat ccaagagtta cttcctcacc
      361 ttgataaagg cgatattttg attgacggag gaaatacatt ctttaaagat acgatgcgtc
      421 gtaatgaaga gttagccaac tctggcatca actttatcgg tacaggcgtt tccggtggtg
      481 aagaaggagc tttaaaagga ccttcaatca tgcctggtgg acaaaaagaa gcctatgcgc
      541 tagttgcacc aatcttagag caaatttctg caaaagcaga agacggagca ccatgtgtca
      601 catatattgg cccaaatgga gccggacatt atgtgaaaat ggtacacaac ggtattgagt
      661 atggagatat gcaactgatt gctgaatctt atgacttaat gaagaatatt ttaaaccttt
      721 cagtagaaga aatggctgat atctttaaag aatggaatca aggcgaatta gacagctatt
      781 tgatagaaat cactgctgac attctgacac gtaaagacga tgaaggaaca ggtaaaccag
      841 tagtagatgt gatccttgat gcagctggga ataaaggaac aggtaaatgg acaagccaaa
      901 gcgcgcttga cttaggtgtg ccacttccat tgatcactga atctgtattt gcccgctata
      961 tttctgctta taaagaagaa cgcgtacaag caagcaagat tttgtctcgt acgaacgatt
     1021 ttgagttcac gggagacaaa aaagaattgg tcgaaaaaat ccgagaagcg ttgtatttca
     1081 gtaaaatcat gagttatgcc caaggatttg ctcaattacg tgtcgcttca aaagaatttg
     1141 attgggactt gccatttggt gaaatcgcaa aaatctggcg cgctggatgt atcatccgag
     1201 ctcgattctt gcaaaaaatt acggatgcct atgacaaaaa tcctgagatc gaaaatttat
     1261 tgctagatga ttacttcgtt gaaatcacga aaaaatatca gcaatcagtc agagacgtcg
     1321 ttgccttggc tgtccaagct ggcgtacctg taccgacttt ctcatcagcc atcgcttact
     1381 tcgattcata cagagcagaa cgtctgcctg caaatataat ccaagcccaa cgcgattact
     1441 ttggcgcaca tacctatgaa cgtgtcgaca aagaaggaat cttccattac tcatggtacc
     1501 atgaagaata atagtctttc ggctgttagg aaatgagaaa ataaaaagag gacgaaagag
     1561 ctgaagtcga ttgttatgag tagacttcag ctctttttta aaattgctat ccatgataaa
     1621 atgactatag atttttaagc atattatcgg tataatgata gagtttttaa aaagccagta
     1681 gaatagaaag gataaaataa taaatgagca atattctaat cattgaagat gaaaagaact
     1741 tggcacgatt tgtcgagcta gagttgaagc atgaagggta taacgcagaa gtccactaca
     1801 acggccgaac tggtttagat gcggcattaa ataatgagtg ggatgccata ttgttggatc
     1861 tgatgctccc agaattgaac ggtttagaag tctgcagacg tgtacgccaa gtcaaaaata
     1921 caccaatcat tatgatgact gcaagagatt ctgtgatcga tcgtgtatct ggacttgacc
     1981 acggagcaga tgactacatc gtcaaaccat ttgctatcga agaattatta gcacgtcttc
     2041 gtgcattgtt gcgtcgaatc gatattgaag gcgataaaaa tgtggctaaa caaactacta
     2101 ttacttatcg cgatttgact atcgaaaaag aaaaccgtgt cgtacgtcgc ggaaatgaag
     2161 taattgaact aacaaaaaga gaatacgagt tgctgcttac tttaatggaa aacgtgaacg
     2221 tggtattagc acgtgacgtt ttactaaata aagtgtgggg atacgaaaca gaagttgaaa
     2281 caaacgtagt ggacgtatat attcgttatc ttcgcaacaa aattgacgtt cccggggagg
     2341 aaagctatat ccaaactgta cgcggaacag gttatgtgat gcgctcgtga taaagccgaa
     2401 aaaatcggaa aaaagagaac tgaaagggcc ctccttgaca atcaagtggg cctttgcgag
     2461 ttctttcttc atattcgttg tgtttacaat tttcgctgtc attacctata aatcatctat
     2521 caatctgatt gtggcaaaag aaagaaacaa tgtagagcgt acgatttcag aagtagcttc
     2581 tcgtttggct aactcggatg aagaactgac attagtaaat acgtatcgta atctgacgaa
     2641 agcgacaaat gacggagatg aggtcgtaga tcatacttca ctggtcgaag gaaacttaat
     2701 gaagatcgat tcctttatag ctgagctagg gcagccatca atggatcttt atgtttatga
     2761 tttggacgag aacttgattt ttaaaaccca tgaaaacagt cgaaacctga ttcagactac
     2821 acgtaaagtt ccaacgatcg ttacactaga aggaaaaact ggctttttat ctatccagcc
     2881 tatatattct aaaggtacaa gagaaaagat cggctatgcg caaagcttct atgaattatc
     2941 ttctttctat gatattcgaa ataagctatt attgactctt attgttttag aagttgtttc
     3001 tttgatctta agtagtattt taggtttctt tttatcttct tatttcttaa aaccactcaa
     3061 ggtactcaga gatacaatgg atactatacg taaagatccg caatcagata tccacatgcc
     3121 tgaaatcgac acaaatgatg aattggcaga tttagctgaa atattcaatg agatgcttga
     3181 ccgtatgcgt ttgtatattg aacaacaaga gcaattcgtg gaagacgtat ctcatgagtt
     3241 aaggacacct gttgcaatta ttgaagggca tcttagtctg ctaaatcgtt gggggaaaga
     3301 tgatcctgag atattagaag aatcattaag cgccagcatg caagaaatta gccgtatgaa
     3361 aagcttagtt caagagatgc tagacttatc aagagcagaa caagtagatg ttcattatgc
     3421 gaatgaaaca actaaaggaa aagaagtcac ctatcaagtt tataataatt tcaagatatt
     3481 atatccagac tatgtgatca ctttagatga tgatttgaca aaagaagtga ccctgcaaat
     3541 ttatcgtaat cactttgaac aattaatcat tatcatattg gataacgcag taaagtattc
     3601 aactacacga aaagaagtcc atatctctat ttcttccact ctgaatgaat ttgagatcgc
     3661 gattcaggac tttggcgaag gaatacccaa agaagacctt aataagatct tcaatcgttt
     3721 ttatcgagtg gataaagcaa gagctagaac gaagggtgga aatggtctgg ggttgtctat
     3781 cgctaaacaa ctggttgaaa actataaagg acgtatctta gcagaaagtg tagtagggca
     3841 agggacaatt tttagaatct ttattcctat cgcaaaggca gaagacaagt aaagcatgtg
     3901 ctttacttgt ttttttatat atccaacaaa gaataaggta caaaatacaa gcaagttaga
     3961 aattcaggga tgtcattata aaaaaaacta gcgaacaatt aaatagaata acatccgtta
     4021 attaaacgta agtagctttt agatgaacga aaaaacaatg aaatgctgta atattcgtgt
     4081 ttaaaaataa taaataaaaa tagggaattt tctattgcaa ttttataaag agattgctat
     4141 attattattt gttctaaatg attcctttaa ggtgtcacta aaaattataa aaaatagggt
     4201 tgacgcactt ttgatgtcat gttataataa cgaagtcgtt tggatgaggt agctaataaa
     4261 gcttgatttt atcgaaaaaa acttcgaaaa agttgttgac aaataacaat aaagatgata
     4321 tgataatcaa gtcgctgcta atgagtgaca aagacgttta gaaaaaataa acaaagaagt
     4381 tgttgacatt ctcttctgaa tctgataaga ttaaagagtt gttgaaacaa aaagattaga
     4441 cctttgaaaa ctgaacaaag taagacaaac caaatgtgta gggcgtcttg attcaattca
     4501 agacaacaaa catttttaac aagcaagcaa tatgctagca aacaaattga gcttaacaat
     4561 cgcaagattg ttcgaacttt ttatgagagt ttgatcctgg ctcaggacga acgctggcgg
     4621 cgtgcctaat acatgcaagt cgaacgcttc tttttccacc ggagcttgct ccaccggaaa
     4681 aagaggagtg gcgaacgggt gagtaacacg tgggtaacct gcccatcaga aggggataac
     4741 acttggaaac aggtgctaat accgtataac aatcaaaacc gcatggtttt gatttgaaag
     4801 gcgctttc
//
LOCUS       DAZABD010000121         4703 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000121 DAZABD010000000
VERSION     DAZABD010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4703
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.126"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            106..357
                     /locus_tag="ACHALG_002777"
                     /pseudo
     CDS             106..357
                     /locus_tag="ACHALG_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            450..1244
                     /locus_tag="ACHALG_002778"
                     /note="aph(3')-IIIa"
     CDS             450..1244
                     /locus_tag="ACHALG_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HFN2588081.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            1654..1737
                     /locus_tag="ACHALG_002779"
                     /pseudo
     CDS             1654..1737
                     /locus_tag="ACHALG_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502600.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MLS leader peptide"
     gene            1786..1869
                     /locus_tag="ACHALG_002780"
     CDS             1786..1869
                     /locus_tag="ACHALG_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HFN2588082.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            1994..2731
                     /gene="erm(B)"
                     /locus_tag="ACHALG_002781"
     CDS             1994..2731
                     /gene="erm(B)"
                     /locus_tag="ACHALG_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFN2588083.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(2994..3857)
                     /locus_tag="ACHALG_002782"
     CDS             complement(2994..3857)
                     /locus_tag="ACHALG_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFN2588084.1"
                     /translation="MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAI
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLEKELNRKVSGKEIQPTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTP
                     PIPKTPKLPGI"
     gene            complement(3859..4131)
                     /locus_tag="ACHALG_002783"
     CDS             complement(3859..4131)
                     /locus_tag="ACHALG_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFN2588085.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            complement(4148..4363)
                     /locus_tag="ACHALG_002784"
     CDS             complement(4148..4363)
                     /locus_tag="ACHALG_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFN2588086.1"
                     /translation="MIVGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(4455..>4703)
                     /locus_tag="ACHALG_002785"
     CDS             complement(4455..>4703)
                     /locus_tag="ACHALG_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
                     /protein_id="HFN2588087.1"
                     /translation="FVPYLVDTDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKVSTW
                     SKNGITEHKGYDKKVLSMYKNVFFEMLERIIQLENEKE"
BASE COUNT         1443 a          793 c          864 g         1603 t
ORIGIN      
        1 ttcttgggag ttttgagact actatatgcg tttttttgtt gatttaccaa catgtttaga
       61 cattttcctc taggaaaaaa ttaactagca ccacgggtta tcttttatat agaagatatc
      121 gccgtatgta aggatttcag ggggcaaggc ataggcagcg cgcttatcaa tatatctata
      181 gaatgggcaa agcataaaaa cttgcatgga ctaatgcttg aaacccagga caataacctt
      241 atagcttgta aattctatca taattgtggt ttcaaaatcg gctccgtcga tactatgtta
      301 tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaag gttttagaat
      361 gcaaggaaca gtgaattgga gttcgtcttg ttataattag cttcttgggg tatctttaaa
      421 tactgtagaa aagaggaagg aaataataaa tggctaaaat gagaatatca ccggaattga
      481 aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg
      541 tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata
      601 aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc
      661 tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga
      721 gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga
      781 ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc
      841 cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc
      901 tggccgatgt ggattgcgaa aactgggaag aagacactcc atttaaagat ccgcgcgagc
      961 tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc
     1021 tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga
     1081 gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg atcagggagg
     1141 atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt
     1201 gggagaaaat aaaatattat attttactgg atgaattgtt ttagtaccta gatttagatg
     1261 tctaaaaagc tttaactaca agctttttag acatctaatc ttttctgaag tacatccgca
     1321 actgtccata ctctgatgtt ttatatcttt tctaaaagtt cgctagatag gggtcccgag
     1381 cgcctacgag gaatttgtat cgataagaaa tagatttaaa aatttcgctg ttattttgta
     1441 catttaactt gacggtgaca tctctctatt gtgagttatt agtggtacag ttttcaaccg
     1501 ttttaattat aaaaaagtgg tgcattttta aattggcaca aacaggtaac ggttattgca
     1561 ggtgtatttc ttatctatgg gtttaacatg gattttatca ttaaaatcat gagtattgtc
     1621 cgagagtgat tggtcttgcg tatggttaac cctaaagtta tggaaataag acttagaagc
     1681 aaacttaaga gtgtgttgat agtgcattat cttaaaattt tgtataatag gaattgaagt
     1741 taaattagat gctaaaaatt tgtaattaag aaggagggat tcgtcatgtt ggtattccaa
     1801 atgcgttatg tagataaaac atctactgtt ttgaaacaga ctaaaaacag tgattacgca
     1861 gataaataaa tacgttagat taattcctac cagtgactaa tcttatgact ttttaaacag
     1921 ataactaaaa ttacaaacaa atcgtttaac ttctgtattt gtttatagat gtaatcactt
     1981 caggagtgat aacatgaaca aaaatataaa atattctcaa aactttttaa cgagtgaaaa
     2041 agtactcaac caaataataa aacaattgaa tttaaaagaa accgataccg tttacgaaat
     2101 tggaacaggt aaagggcatt taacgacgaa actggctaaa ataagtaaac aggtaacgtc
     2161 tattgaatta gacagtcatc tattcaactt atcgtcagaa aaattaaaac tgaatactcg
     2221 tgtcacttta attcaccaag atattctaca gtttcaattc cctaacaaac agaggtataa
     2281 aattgttggg aatattcctt accatttaag cacacaaatt attaaaaaag tggtttttga
     2341 aagccgtgcg tctgacatct atctgattgt tgaagaagga ttctacaagc gtaccttgga
     2401 tattcaccga acactagggt tgctcttgca cactcaagtc tcgattcagc aattgcttaa
     2461 gctgccagcg gaatgctttc atcctaaacc aaaagtaaac agtgtcttaa taaaacttac
     2521 ccgccatacc acagatgttc cagataaata ttggaagcta tatacgtact ttgtttcaaa
     2581 atgggtcaat cgagaatatc gtcaactgtt tactaaaaat cagtttcatc aagcaatgaa
     2641 acacgccaaa gtaaacaatt taagtaccgt tacttatgag caagtattgt ctatttttaa
     2701 tagttatcta ttatttaacg ggaggaaata attctatgag tcgcttttgt aaatttggaa
     2761 agttacacgt tactaaaggg aatgtagata aattattagg tatagggcac ctctaataac
     2821 taccaattaa ttcacctcaa aatgaaatta gaaaaaaaca gcgaaatttt taaatctatt
     2881 tcttatcgat acaaattcct cgtaggcgct cgggacccca acatgaggaa atgtttttat
     2941 aaaaaaagag tagttaccgt tttttggtaa ctactctttt ccttttttaa ggtttaaata
     3001 cctggaagtt taggtgtttt gggtattggt ggtgtaggtg gctgcaagct ttccaatttt
     3061 tgttgaattg ctttaaattc aggtgtctct tggtgtttat ttagaaccat tttttgctct
     3121 attcgttcta aagtcggttg aatttctttc cctgatactt tacgattcaa ttctttttct
     3181 aaggtttctt ttggactaat agaaggtgtt tccaaacttg aatagagttt tactccttct
     3241 ctgttatata gccttatatc gctaaataag cccgttttat gaagggtttc taaattggtc
     3301 ggtaagtttt tgacaacaat atcatgcgct tgttttggtg ttgccctggc tgtcattgga
     3361 tcatctgcat acatggtttc atatcgttca attgttccta aatatgagtt gattttaggt
     3421 actgccatga catacatttt tgtttcataa cctttggctt gaagcattgt tgcggtttga
     3481 ataggaacgt ctgttgttcg tcctgtacct tcgatcacca aattataccc ttgatcgctc
     3541 aaacggctta tgatcgcttc tgtcatgcga ttagaataag gggtaacgtg ttttactacg
     3601 tctttttcat aaagtttcac tagttcatca aaattagggt gctgttgttt aaaggtatca
     3661 ttatcaatga caataacatt cccttgtgtt tcttcaaaaa ttgctgatcg caaactggtt
     3721 ttccctgacc ctggttgccc accaagtaaa aaagcggttg gcgattcaac cgcttttttt
     3781 ccttgaatca attcttctaa attatcattt aagcgattct caaattgttt gtcagtaaaa
     3841 ttgactatat ttgccatatt aagccacttt ctctttattc aaaacttgtt ttgagtaacg
     3901 attcattgac cgccaatact catgaacggc ttgtaattct tctaaagaat aatcaaaaag
     3961 atttacacgt tttgcaagct ttaattggtt taataaattg acttccaata attgagagtc
     4021 atttttattt aattcatggt tcaaaacata gtttaatact cgattataaa cgcttgccga
     4081 taattcgtta atgatctcta tttcaaatgt tttttcatac gtaactgcca tttcattttc
     4141 actcctttta aagtttgtca ggtaaatatt tccgtatata ttcttctaag gcttgatcca
     4201 taacttcttt tacgtttccg ccattctttg ctgtttcgat ttttatgata tggtgcaagt
     4261 cagcacgaac acgaaccgtc ttatctccca ttatatcttt ttttgcactg attggtgtat
     4321 catttcgttt tgctttttgt gcgcctaaat ttcccacaat cactcacttc tttctatttc
     4381 ttcttactct tattttatca tcaacaatca caaatcacaa gtgatttgtg attaatcact
     4441 tgtgatttgt gattctattc tttttcgttt tctaattgaa taattcgctc aagcatttca
     4501 aaaaatacgt tcttatacat ggataaaact tttttgtcat agcctttatg ctctgtaatg
     4561 ccgtttttag accaggtact tactttatta cttcgcttga taatattttg gaaaaccaag
     4621 ttatcttctt tatgttgctt atacagttct tccaggtttg attttatcgt tgcgctgtcc
     4681 gtatcaacta aataaggaac aaa
//
LOCUS       DAZABD010000122         4467 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000122 DAZABD010000000
VERSION     DAZABD010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4467
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.127"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..790
                     /locus_tag="ACHALG_002786"
     CDS             <1..790
                     /locus_tag="ACHALG_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFN2588088.1"
                     /translation="CGYSKTEPRHSFGPAIREQYLIHIVLEGEGYYSINDQKYFLKKG
                     QGFVIPPGVSTFYQADEKNPWSYVWLGLSGNLVEYYLKEIGVGKNHLTFKVENLNSFK
                     GLIFETLSYDQDNLVSELSRQKQVYKFLELLLNSASYLNEGIITKKMNPFVRQTIEII
                     RENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISIKEYISEIKISIANDLLSSTEYS
                     IQQISDKIGFSTVQSFSKAFKKANGVSPGVYRKRRLGLGIINGE"
     gene            887..3136
                     /locus_tag="ACHALG_002787"
     CDS             887..3136
                     /locus_tag="ACHALG_002787"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFN2588089.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKISEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
     gene            3139..4314
                     /locus_tag="ACHALG_002788"
     CDS             3139..4314
                     /locus_tag="ACHALG_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFN2588090.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTRVTRGAS"
     gene            complement(4317..>4467)
                     /locus_tag="ACHALG_002789"
     CDS             complement(4317..>4467)
                     /locus_tag="ACHALG_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HFN2588091.1"
                     /translation="LEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQRRFEGTLR
                     YSLGY"
BASE COUNT         1556 a          645 c          816 g         1450 t
ORIGIN      
        1 ttgtgggtat tcaaaaacag aacctagaca tagttttgga ccagctattc gagaacaata
       61 cttaatacac atagtgctag aaggtgaagg ctattattca attaatgatc aaaaatattt
      121 tctgaagaaa ggccaaggat ttgttatccc accaggagta tctacttttt atcaagcaga
      181 tgaaaaaaat ccgtggtcat atgtatggtt gggtttaagt ggcaatcttg tagaatacta
      241 tctaaaagaa attggtgttg gaaagaatca tcttaccttt aaagtcgaaa atttaaattc
      301 atttaaagga ttaatttttg aaactttatc gtatgatcag gacaatctag tcagtgaatt
      361 gtccagacaa aaacaagttt ataaattttt agaactcctc ttaaattcag ctagttatct
      421 gaacgaaggg ataattacta aaaagatgaa tccttttgtt cgtcaaacaa ttgaaataat
      481 acgtgagaat gcgtatcaaa aattatctgt taaagatatt gctgtcagtc tatcgataca
      541 cgaaaattat ttatcacgat tgtttaagaa tgatatggga atatcaataa aagagtatat
      601 tagtgaaatc aaaatttcta ttgcaaacga tctgttatca tcaactgaat attcaatcca
      661 gcaaatcagt gacaagatag gattctcaac agtacaatct ttttctaaag cctttaagaa
      721 agcaaatggt gtttcaccgg gagtatatcg aaaacgtaga ttaggtctag gaatcattaa
      781 tggtgaataa actaaacatt tgattttgat aaatattcgt tactttaaac gcttactatt
      841 tatgtgagcg ttttttataa tgcaataaag aaacggagga gagcttatgt ataaggataa
      901 tgaaatgaat attatttatc gatcagaaaa ccaacaattt catttgttta atcaggagat
      961 aagttacatt tttaagatat cggaagatgg taaattattg catttatatt atggtgagaa
     1021 cttaccagat aatgactata gtcatctaat tgagatgcat catcggccta tgacaaccta
     1081 tagaaatgaa aacgatttgc tatattcgtt agagcactta aaacaagaat ttcctgaata
     1141 tggtacaaca gattttagac atcctgctat ttcattacta cagaaaaatg gctctcgctt
     1201 atcagatttt gtctatcaag ggtacgaagt ggaagaagga aaacctaaac tagaaggttt
     1261 acctgctacc tatgttgata ataatgatga aagcaaaaca ttgaaagttt atattttaga
     1321 ttcagttaca aatgttcaag tagaattatt atatacaatt tatagagatt atcctgttat
     1381 tactcgtagc gcgcgagtta tgaataaagg aaaagatatt caaataatcg agaatattag
     1441 cagtttatca ttggatttac ccgatgcaaa ttatgaatgg caacaattat ctggtgcttg
     1501 gggtagagaa agaactatta agtcgcgtac tttacaacaa ggaattcaat caatcgaatc
     1561 tactcgaggt atttcaagcc atcagcataa cccatttgtt actttaaaaa gaaaaaatac
     1621 tgatgaaaat caaggagaag ctattggtgc agcactagtt tactcaggaa acttcttaat
     1681 tcaagcagaa gtagatacat gggatgttac tagactacaa gttggcatca atccatttgg
     1741 atttaattgg aaactcaatc caggagaaat atttacagca ccagaagcga ttctggttta
     1801 ttcaagtaag ggtttaaatg ccatgagtca aacttttcat caactattta gaaaacgctt
     1861 agctcgtggt aagtggcgag aaaaagaccg tcctgtctta attaataatt gggaagcaac
     1921 atattttgat tttgacgaag aaaagttggt aaatattgct aaaaaagcat ttgatgttgg
     1981 tattgaatta tttgtattag atgatggctg gtttggagaa agagaaaacg attgtgcagg
     2041 attaggagat tggcatgtaa atcctaaaag actacctaga ggaatcggca gcctttcaga
     2101 aaaaattcgt agttttggta tgaaatttgg tttatggttt gaacctgaaa tggtgaataa
     2161 ggatagtgag ttatatcgca cacatccgaa ttggattatt tctacaccta atcgaaaacc
     2221 taatcatggt aggaaccaat atgtattaaa ttttgggtta gatgaggttg ttgaaaatat
     2281 tttcgatcaa atgtgtaaaa tcattgatga atcaaactta gattacatta agtgggatat
     2341 gaatcgtcca ttgactgatg tatttgacag tcacttgtca gcagatcaac aaggagaagt
     2401 attccacaga tatgtactag gcgtatatcg cttatatgaa aaacttataa caaaatatcc
     2461 taacatacta tttgagtctt gttcatcagg tggtgggcgc tttgatcctg gaatgcttca
     2521 ctatgcacct caagcttggg ctagtgatga ttcagatgca attgaaaggt taaaaattca
     2581 atatggaact agtatgctgt atcctctttc gtctattggt gctcatgtat caattgttcc
     2641 aaatcatcaa acaaaccgta taacacctat aaaaactaga ggaaatgtag ctttttttgg
     2701 tgcatttgga tatgagcttg acttaaatga attaagtgaa ggcgatttga ggattgtaaa
     2761 agaacaaatt gccttttata aaaaatatag aaccataatt cataatggta ctttttatag
     2821 actattatca ccattcgatg atgataatca aaccgcgtgg atggttgtta gtcatgataa
     2881 gaaaacggct attgttgctc attataaaac attaaatgag gtaaatgctc cttaccgccg
     2941 actgaaatta aaagggttag ataatgacgg actttataat gtaggacaaa atagtttgcg
     3001 atctggaatg gaattgatga gagcaggact agtctgttca gatgcatcta gtggacaaat
     3061 aaatgatcag aatcgacgcc cagaaacatt tgattttgat tccaagattt gggttataac
     3121 acaggaggaa aagtagagat gaataaccaa aaaagcttac ttcaaaaagg agcaatctta
     3181 agtatttcac taatgttaac aagctctcaa gcaattaatg gtgttattcc acaaatgaaa
     3241 cagtctttgg ggatatcaca aagtcaaagt gaacttttag gaactgcacc aagtataact
     3301 gttattttgt ttatcttatt atctagttac tttgccaaaa gaataggaat gaaaaagaca
     3361 ataattttag gtttattatt agctggtata ggaggaatta ttccagtatt tacagctacc
     3421 ttccaaaccg ttcttattag tcgagtaata ttaggtgccg gtttaggagt atataattca
     3481 ttggctgtga catatatcag ctctttatac tctggcaata ctagagcaac gctattaggt
     3541 attagaaatt caatggaagc tttaggtcag acaatcctta tttttcttgc tggagtttta
     3601 gtgaacatta attggacggc ttcgttttta gtctatgcaa ttgcgtttcc tgtagctctc
     3661 ttattcgcgc taaaagttcc tgaaattaga gatgaaaata gtgacatacc tgaaaatcat
     3721 gtaaaagaaa agatgaatcc aatggttttt gccctagttc tatttgcaat cttactcgta
     3781 atgaatagta ttgctatttc tgtacgattt gcatcaattg caactgaaat taaaggggta
     3841 aactttaatg ctagtaacta tttagctcta atgcctatct taggaatact agcaggattt
     3901 atgtttggac caataaataa gtggatagga aaaggaaccc tatacttggg aattatattc
     3961 tttataattt caaatttgtt aattgctgtg tcaaacggta atatgacgtt cctgctaacg
     4021 ggtttatttt tatcaagtat aacaggatca tggtgcttcc catttatctt tagtaactta
     4081 gataaagtca ctacaaagaa tacaattaat tttgctactt cattgatttt tattggctgt
     4141 aatattggta attttatagc tcctattgct atgcaattag cacaatttct aacaagaagt
     4201 acaaatttga ctgctccatt ttttgtattc gcaggaatat ttattgtgat cctatttgta
     4261 acattttttg cgacaaataa aaaaaatact agagtaaccc gtggtgctag ttaattttaa
     4321 tatcctaaag aatatctcaa agttccttca aaacgtcgtt gtgctcgact taatagaaca
     4381 ctgtaagcca gtaatatgct ttcaaatcta ctctctagcc ctttaacaga acgtgagttg
     4441 aaattttgac aacctaattt ttctaag
//
LOCUS       DAZABD010000123         4425 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000123 DAZABD010000000
VERSION     DAZABD010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.129"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            690..1658
                     /locus_tag="ACHALG_002790"
     CDS             690..1658
                     /locus_tag="ACHALG_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFN2588092.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            1726..2484
                     /locus_tag="ACHALG_002791"
     CDS             1726..2484
                     /locus_tag="ACHALG_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFN2588093.1"
                     /translation="MYPGGNSLNFAVFAKKLGHESSFMGVFGSDEEAAHVLSAIKEIG
                     LDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREYPIQLTEEDREYLNQFELIH
                     MGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAIDKVDFGFFSIEDFSDEEVKQ
                     FLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEEPVDTMAAGASFLTAFLLYF
                     LEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2552..3262
                     /locus_tag="ACHALG_002792"
     CDS             2552..3262
                     /locus_tag="ACHALG_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFN2588094.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            3298..>4425
                     /locus_tag="ACHALG_002793"
     CDS             3298..>4425
                     /locus_tag="ACHALG_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFN2588095.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT         1489 a          662 c          921 g         1353 t
ORIGIN      
        1 atacaaataa ttcaccaaaa aggtgagtaa tgccattttt gaaaattgag cttgttttat
       61 gtctacatct gtatccgtat ataatccaaa ggattataaa ctcagaagag tctttatttt
      121 ttctgaatat aactactgta tcaaaaacaa tcgattatga gacaaaacaa aatgacctct
      181 tttatttaag aaaggaggtc attttttgta tcataaattg tattaaaatc aatgtatcac
      241 tttttaatca gttttaagac cataaattgc gtactaaaag aaattctgca attcaaaata
      301 aagcatttta agccattttc aaatttaagt gcacccatct acccgtcaaa tcactttatt
      361 ttccactcca gtagtttcct aattcaactg tgtgcaaaaa cgatttcttc taaacaaatc
      421 aagacaaact tataaacctt catcccagca ccataattat ttaaatcata ggcatttttc
      481 gaaattagta tatgtactca agtttcactg cagattattt atatttttta gagaaataaa
      541 tttttcctat tgacaaacac aaagaaagcg gatacaatgc aattatgtca catgacgtta
      601 tatgacgtta tgtgatatct ttacatttaa tcagttactt ttattttgga taaaattaac
      661 atttttaaaa ttttaaggag gaaatgttga tggatgtagc aaaaacggtt aacgcaattg
      721 tagaaaaaaa tgatggagag atttctggaa tttatttggt ggcttgtggc ggttcgctgg
      781 tagatatgta tgtctcagat tattttctta aagccgaagc atcgaagtgt attacaggct
      841 tatatacagc gaacgaattt aaccatgtaa ccccaaagca attagatgat aaatctgttg
      901 tcattctatg ttctcatgga ggaaatacag cagaaacggt aaaagcagga gaagtcgctc
      961 aagctagagg agctgtgact gttggtctga ctcataataa aactgcggat ttattaaagg
     1021 tttctgatca tagtttcctt tacgaatggg gagatgatgc aaaaatagtc aataatccta
     1081 tggccatcat tcttgattta actgtatctt tggtcaatgt taccgaaaca tttgaacatt
     1141 acggtaaatt cagagaaggc atgaaggtta tcgataccgt aattgaaaat ggcattaagc
     1201 aggtgcagga tcgtattaaa gtgtttgctg ataagtacaa agatgaaaca atgttttata
     1261 tcttaggtag tggaccatca tatggacatg cttatggttt ttcaatttgc tctttgatgg
     1321 aaatgcaatg gcttgattca acttctgttc attctggaga attcttccat ggaccttttg
     1381 aagtaacaga caaagatacg aattttattc tcttgatgag cattggacgc actcgtgctt
     1441 tagatgaacg ggcaaaacga ttccttgata aatatgcaga aagagtagaa atcgttgatg
     1501 caaaggaact aggattggat atgatatcag atgaagtttc tgaattcttc aatcccattt
     1561 tattctacag tattctttca gaatatcgtt cagctttagc agatgtacgc aatcatgatt
     1621 tagatgttcg tcgctatatg gggaaagtgg agtactagga ggattaaaat gaaggtatta
     1681 ggcttaggtg ataacgtcgt cgataaatat gaaaatctgg gaattatgta tcctggagga
     1741 aattcattga attttgcagt ttttgcaaaa aaactgggac atgaaagtag ctttatggga
     1801 gtttttggct cagatgagga ggcagctcat gttttatcag caataaaaga aataggactg
     1861 gacaacagtc actctcgctt tgaaaaaggc gagaatggct gtgccagagt gaagattgat
     1921 gagggcgacc ggattttcct tggcagcaat tcgggaggtg ttactcgtga atacccgatt
     1981 cagcttactg aagaagacag agaatattta aaccagtttg aattgattca tatgggatta
     2041 tatagccatg tcaatcatct attagaagag ttacaaaatg tttctggaaa gatttcttat
     2101 gatttttctg acgactttac ggaagaagag gttcagcggg caattgataa agttgatttt
     2161 ggcttctttt ctatagaaga cttttccgat gaagaagtaa agcagttttt gatgaagcat
     2221 ttttgtgagc gaaatgatgt attgattgca acccgaggag aaaaaacggc tattggttat
     2281 gatggagcaa agttttatga agtgaacccc gtgctagaag aaccagttga tacaatggca
     2341 gcgggagctt ctttcttaac agctttccta ctttattttc tcgaagggga cgatatagtc
     2401 accgcgatga aaaaggggaa tgaatttgct gggaagtctt gtctggttga cggttcgttc
     2461 ggctttggta ttcattacga ttgagcttgt tttaaattcc tgcatttgat aacctaaaga
     2521 aagaaatccc ggtaaagtgg aggttaaaaa aatgctggat acaaatgcac aattaccatt
     2581 gtacttacaa ttaaaagaaa tattaaaaaa taaaatcaat acaggggagt atcccgagaa
     2641 tacgaaaatt cccgctgaat cagagctgat agaaagttat gaggtcagcc gaatcacagt
     2701 gagaaaagca gtagaagaac tggtagaaga agggtaccta cttaaaaaac aagggaaagg
     2761 aacttttgtc aaccggcata aagtttttcg taaaattgaa tatattatgg gtttcagtga
     2821 gagttgtaaa tctaatggtt ttacaccgac tagcattgtt ttggaacgca agatcattcc
     2881 agcaactgaa gggatggcaa aaaaactcga tatcgctcaa ggtgatgatg ttttatatat
     2941 ccagagaaag cgaatggcgg ataacatccc cattatgtta gaaaacaatt atttttctta
     3001 tgaaagatat agtttcttaa tggaggaaga gctgactgat tctttgtatg aagtacttaa
     3061 agtccaaggt accttagcca tcaatcctgg ggaaactgca cttgaaattg tcacagctga
     3121 tgaccaaatt gccaaaataa tggaagtagc tgttgggacg ccctttttct ttatggatac
     3181 tttaatcaac gatcaaaaca acataccaat tcatgtaggt aatcaatatt acctgggaga
     3241 agtatatcgt ttttacttat gatttcctgt ttggatttca aggagtagat tttatttatg
     3301 aagcaaatat tgcggtcaaa aatgattttt gatggagagc agccaccttt caaaggttat
     3361 attgtaatca atgaagggat cattgaaaag gtggaaaaca actggaatta cagtgatcta
     3421 ttaaatgaaa acacggaatt aattgcttat gatgatcaat tcgttatgcc agcaattcat
     3481 gataatcatg tatttttcag tggttatttg gctatgaatg ctggcataga tttaagcgat
     3541 acatcttcag ctaacgaggc tgtagagcgt attaaagctg taatgaacca taaaaaagca
     3601 gatgaaccaa tttatgctca tggctgggat gctgccctgt ggaaaactga cccatcagaa
     3661 gagctattga atgaaataaa ccactttggt ccaatcacag cgatcgacaa aaatcgcagc
     3721 cattgttgga tgaatgctat tgctaagcag acttatggtt ttacagaaaa agaaaccagt
     3781 gctgaaagtc gagtattatt aattaaggaa atgctagcaa acaaaaagct tttagcagag
     3841 gtttacgctg aatttgaagc attactgctt tcaaaaggtg ttatatcaat gaaagaaatc
     3901 gtttttgatg acgcagaatt tctttctaat atacctcaga ggaaaatact tagtaaccta
     3961 tatgtgcagg cagtccaaga acctctacag catgaacttc tatatgagta taaggaaaaa
     4021 gttttccctg aaaaggtccg ctttggtgga gtgaagatca tggtagatgg tgttgttgcg
     4081 gatagaagtg gagatattta cggtgtttat cagtcaggag tagcttcacc cgagattgac
     4141 tatgaggcaa ttaaagcaga agttgctgca ttcaatgaag tagagattcc ttgctgtttg
     4201 acaacggaag gagataaagc gggttataaa gctgcgtcaa ttcttgtcga atttggaaag
     4261 cggttacctg aaggcgtttt taattcgatt agtgatttgg aaatggttac tcaaaagaca
     4321 gcagaaaaaa tgaacgaagg acaagtagta gcagaggtat atccacaaat tctcggcttg
     4381 aatcaaacgt tagaagaggc ttacatgtca actgttataa aaaat
//
LOCUS       DAZABD010000124         4349 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000124 DAZABD010000000
VERSION     DAZABD010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.130"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..150
                     /locus_tag="ACHALG_002794"
     CDS             <1..150
                     /locus_tag="ACHALG_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN2588096.1"
                     /translation="KNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDI
                     GAANK"
     gene            233..1351
                     /locus_tag="ACHALG_002795"
     CDS             233..1351
                     /locus_tag="ACHALG_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFN2588097.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            1372..3243
                     /locus_tag="ACHALG_002796"
     CDS             1372..3243
                     /locus_tag="ACHALG_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HFN2588098.1"
                     /translation="MKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQIDYIHQGVG
                     YGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYWGEIHERDQR
                     MVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQFFKIIVGTII
                     QKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYGLIYSVLEPE
                     KPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAMLWADLLPFKL
                     GEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLWLNKIFLLLA
                     LPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPNFHTLTNEKY
                     LKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKTYGQFIVRNN
                     VRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLYETGFAIECS
                     PEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLMTWENTFIPG
                     LEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKNNKKRLKLC"
     gene            3237..>4349
                     /locus_tag="ACHALG_002797"
     CDS             3237..>4349
                     /locus_tag="ACHALG_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFN2588099.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQS"
BASE COUNT         1512 a          646 c          934 g         1257 t
ORIGIN      
        1 aaaaatatga cggtttctag tacaggtgag ccaattaatt atggcgcaat tatgctaatt
       61 ttagcaactt cagtgatccc aatggtcttg atttatgtgt gggcgcagtc taaatttaag
      121 gagaatgaca ttggagctgc aaataagtaa aaaaaataac gaatgttaac catctagttc
      181 gttctaggag caactccaca ttcataaaaa ttaaaagaat ggcgggatat ccatgaatga
      241 acagacgaag gaatggtcaa gaaaaatttt gaatgtagct tctgaaaaga tagatgcgga
      301 attagacaga atggggaata aaattccata tatttcttac aacggaaaat atacagacgt
      361 aataaaggac agaggaatcg actggtggac caatggtttt tggagtggta ttttatggca
      421 gctctttcac aagacaaaac gagaaaagtt caaagaggca gcaatacaac aagaacaatt
      481 attagaatct gccctgtacc atttttcaga cgttcatcat gatgtaggtt ttttattctt
      541 accatcggct attgcacatt atcaacagtc tcaaaacaaa catagtcgac aactagggtt
      601 acatgcagct acggtacttg ctggacgcta taatatttca gggaactatt tagtagcatg
      661 gaatgaccat aaacctggtt ggatgattat tgatagtatg atgaatttac cattactgta
      721 ttgggcttcg agggaaactg gtgatccgag gttctctaaa attgctcggc agcatgcaga
      781 tacaacagct aagtatctag tgaggaaaga tggatcaacc gggcatatcg cagaatttga
      841 tgcagaatca gggaaatata ttcggttact tggcgggcaa ggatacaatg agaattcttg
      901 ttggagcaga ggaaatgcat gggctttata tggattcgcg ttaagttatc gttacacaaa
      961 aaatccgtac tatctagcta tagcagaaaa gatagcagta aattttattc agaaaatcga
     1021 aaaaactggt tatattcctc tcattgattt tgctgtaaaa gattcgaata ttgataccag
     1081 tgctggagta tgcgctgcat gcggtcttct ggaattagca ggatatgttt cctctgtacg
     1141 atcaaaaatg tatcatgaag ctgctatcaa tatactccaa aagacagaaa agcactatgc
     1201 aaattggaat atggatgaag atgggatcat tggaggcgga gcagaagcat accatagaga
     1261 actgacttat aatgtgccac tgatctattc tgattatttt tttattgaag gcttacttcg
     1321 attagaacag tcggaattag ctatttggta gtaaaaagag gagaaaatac aatgaaacat
     1381 ccagtaccac tattaaggac cagacaggat tactttgact tatttaaaaa gataaatcaa
     1441 ccactccagc cgttttatcg agcaagtggc gcacaaattg attatataca tcaaggggta
     1501 ggatatggaa ataaaattgc aggaatggaa ggattctctc gggttttatg gggagcgggg
     1561 cccgcaattg accaattaga tgaacgatgg ttgaaattaa ttctatcagg aataaaagct
     1621 ggaacagatc cttcccatcc cgattattgg ggagaaatac atgaaagaga ccagcgtatg
     1681 gtagaaatgc ccgcaatgtt attagcgtta tatcatcatg aacaattatt atggaaaaaa
     1741 ttagatacag gagaaaaaag ccagattatt cattggttct cgcaaatttt tgaatatgaa
     1801 tgtgcagatg gtaattggca attttttaag ataattgtcg gaacaattat ccaaaaattt
     1861 ggttatccag ttaaagaatc agcactgaaa gaagcatttg ataatataga aaattgttat
     1921 cttcaaaatg gctggtatcg agacagttca agaggtcgcc aagattatta taatcctttt
     1981 gcatttcatt attatggttt gatttatagc gtactagaac cagaaaagcc cattagccag
     2041 atatataaga accgagctat tgatttttct agagactata ttcatttttt tgcggaagat
     2101 ggtgcaaatg tgccttttgg tcgaagcatg atttatcgtt ttgcagtttc aagcttttgg
     2161 tcagctatgt tgtgggccga tcttttgcct tttaaattag gtgaaatcaa ggggttagtc
     2221 aatcggaata taagatggtg gatgcaaaaa gaaatatttg atgaaaaagg tatcctgaat
     2281 attggttata cctaccctca attgacattg actgaaccct ataatagtac gttatctcca
     2341 ttatggctga ataagatttt tttattgttg gcgctacctg aagaccatga gtattggacg
     2401 gctaaagaaa tgcctatgcc tattcgtgaa ggaactaaac ttctttcaga agccaatttg
     2461 ctcatacaac atgataatgg acatacattt tttttgaatg cgggacaatt agggccaaat
     2521 ttccatacat tgaccaatga aaaatattta aaatttgctt attctagtca atttggtttt
     2581 tcgataccgc gagcgaatca attgaaagca gaaatggcaa tggattcaat gctagggatt
     2641 cagcgagcgg acacatatat cacgaccagt tttaagaatg agagcatcaa aacctatgga
     2701 cagttcatcg ttcgaaataa tgtacgagac attgtatgta agaaagaggt acttgcctct
     2761 acatgggaac ctactttaca ggcaaaaatc agaacttggc ttattccttt tgaaggctgg
     2821 cagattcgcg tacacaaagt agaattagat acagaatatg ttctatatga gacagggttt
     2881 gctattgaat gttctcctga gaaagaagga ataatcatag aagaagacaa ggaaaactat
     2941 agggtgagtg aagctggctt ttcaggtatt atctgtttaa gtgacgatac tattaaacga
     3001 aaatccacag ctattatggg tttgcccaat acaaacttga tgacatggga aaacactttt
     3061 attcctggat tagagggtac atttggaaaa ggaacccatt ggctcggaac aggagtggtt
     3121 gctcatcaag atcgggatta tagtttagaa gtatggaaca atcgtcccaa aattgatttt
     3181 gatgggacaa ccgggctaac tatccaaaat aaaaacaata aaaaaagact aaaattatgc
     3241 taaaggctgg tggggaaatg cttgataaag tgatgtttga agagataata catccagaaa
     3301 aatggataaa tgaatgttat aaagaattaa tggaaaaaga atggaataat gagatcgagc
     3361 gttgtcgcag acacacggag aggttaatca aacgtatcag taacagtcag tatcaattcc
     3421 caagcgagtg tcctataaaa ggacgttatc cattaagaga caatattggt tggacagaag
     3481 gattttggac aggaattaat tggttgttat ttgagaagac gggtgatcct ttatttcaac
     3541 aaacagctga aaaagcagat atagatttta tccacaggat tgactgtcaa attatcacta
     3601 atcatcacga tcttggtttt ttgtattctt tatcaaccgt agcagattat aaaataacgg
     3661 gaaatcgaaa aatgaaggaa gcttcaattg atgctgcaga aacattactt cagcgttttt
     3721 gggagaaggc aggagttata caagcttggg gagatcctag tgatccttca caacaaggta
     3781 gaatgatcat ggattgttgc atgaatcttc cattattgta ttgggcgagt gaagaaactg
     3841 gaaatcaaaa atataaaaaa gctgctcagg ctcatattca aaatgcagca gattatttag
     3901 taagagaaga tggttcaact tatcatacat tttatatgga tgtcaaagat ggaaaaccaa
     3961 gatatggaag tacgcatcaa ggttatagtg ataattcctg ttgggctaga ggccaagcat
     4021 gggggattta cggttttgca ttaagttatc gttatttgaa agaacaaaaa tattttgatt
     4081 taagcaaaat attagccaat tattttatga atcggttgcc agaagatacc atttgttatt
     4141 gggatttgat ctttgatgat tctagcagag aagaaaaaga tacatctagt atggtgatca
     4201 tggtttgtgg attattagaa ttaatagaat caggtctatt agacaaaaca gaaaagaact
     4261 tataccagtc tgctgtttgg aaaattatgc gacaattgaa agaaagatat gctacaacgg
     4321 aagaagaaga tggcttttta acacagtct
//
LOCUS       DAZABD010000125         4310 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000125 DAZABD010000000
VERSION     DAZABD010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4310
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.131"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(65..1285)
                     /locus_tag="ACHALG_002798"
     CDS             complement(65..1285)
                     /locus_tag="ACHALG_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFN2588100.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1305..1916)
                     /locus_tag="ACHALG_002799"
     CDS             complement(1305..1916)
                     /locus_tag="ACHALG_002799"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFN2588101.1"
                     /translation="MKMKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELP
                     LFNKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVD
                     KPVMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLID
                     EEQVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1959..2891)
                     /locus_tag="ACHALG_002800"
     CDS             complement(1959..2891)
                     /locus_tag="ACHALG_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFN2588102.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3312..4172)
                     /locus_tag="ACHALG_002801"
     CDS             complement(3312..4172)
                     /locus_tag="ACHALG_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN2588103.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1230 a          866 c          707 g         1507 t
ORIGIN      
        1 agcttaaatc atagataccg taagggactt tattctttat ttaaaacttt gcaacagaac
       61 ctttttattt tacagaagca ctcgaaactg catctactgc agtttcatcg tgtgtgctgg
      121 cgctggttac tgcatctgtt tttccaccat gaacagatgc acttgaaacc gcatcttcat
      181 ttccaacaag ttgttgaccc gtttgttgta aataccactc aatgattggt ttgaaatcaa
      241 gaatgtattc attgattcca gcatactctc cattttcatc atgcattgct tggtaattat
      301 gcacaatata tttttctggc acttgttttg gtacatgaac acgtaccgca tctgttgctc
      361 cgcttcgcag ttgttgaatt acccactcta catttttata cgatttttct gggtgacagt
      421 tggctaatgg attccctacc tgaccgggca cacgagcagc taacatctct ttcgcttctt
      481 ggcgcaaatt ataatataaa aattggttat tattatctgc aaaagttaat tccatcggca
      541 tcgaatctaa aaagtaattg atttgatcca ctgtcaataa cccacgattt aatttgacgt
      601 aagtatttcc agaaacggca ccgactgctt cagaagcttt ttccaaccaa tgatcatctg
      661 acatatcaac atcgggtatt gtcgtagcaa ttgttcctgt cgcatgcaaa tcttctggtg
      721 gaagcttttt aggccctgct aaggtagaca cgactttaat gaattttaca aaacttgcta
      781 atgtactgcc taaaaagtca acagttcgtg gatcaatcaa gcgatcttct tggtcaaatg
      841 cttcttttgc ttttcccaat aaaaattcat ttccgggcat cactaatgca ttcacgcctg
      901 gtgcgtccaa aatttggcgc aagtgtaatt gcgcgcgtga agttccttga tcgtagtaag
      961 aagcacccac aaccattaca ggtttatttt caagtggatg gattttaaat gatagccatt
     1021 caagtgcact tttcaatcct gctggaaccg tatgattgtg ttctggcgta gcaataatta
     1081 ccccatcagc cattaagatt ttgcgattta gctgttgaat agcaggacta tttgtttgat
     1141 catcatcttg attgaacaga ggtaaatctt tgatttcaat gatttctaaa ttaaatagtt
     1201 catgatattg tttttggata aatgttaata atttacggtt atatgaaagt tcagctgctg
     1261 atccgacgat acctattaat ttcatctttg tttgctcccc tcgattaaat cgtttcccat
     1321 gaaaagcttt cggcttcacg tttatttgct tggtgcgcat gttgcaattg actagtgatt
     1381 gtgacaaaga gtaagaaatc tttgaatagt tcacgtaatt ttgcgacttg ttcttcatca
     1441 atgagtcgtc cttgctcatc aaaggcttgg agtgaatgac ccaataaaaa ttctgagctt
     1501 ggcataattc gtgcctttaa ctctggtgaa tccaatattt ggcgcaaatg tgcttgtgcg
     1561 cgtgaagaac ctaacgtgcc ataagacgct ccggtaatca tcactggttt atccacaaac
     1621 ggaaaaatac catatgaaag ccaatttaat gcgttaatca atgaagcagg aaccgcatgg
     1681 tcatactctg gtgtgctgat gattacgcca tctgcttgtt caatttttcc agcaatttct
     1741 ttaacaacat ttggtagttc tgtgttttca ggtttgttaa atagtggcag ctccttgatt
     1801 tcgacaattt cgatttctgc ctcatttaca aaataatttt tcataaactg taataattca
     1861 cggtttgttg accgcgttga atttgttcct actaatccaa taaatttcat cttcattctc
     1921 tttcctttct aacatcgatt tctgactgtt cgcacgtact aaattttttc aaatcctttt
     1981 gtatacaaga attgattctc ttttgttaca actgctccct cgatacccgg ctgttcttca
     2041 agcgctttca acacttctaa agaagatgct ccaaataaac gtgtagtcca gatttctcca
     2101 tctacagaag aatcagacaa aatcgtcagg cttgctacgt catttttaac tggaaagcca
     2161 gttttcggat caatgatgtg atgatacact gacgaaccat tttgtagata acgttcgtag
     2221 attccagagg taactaaaga ttggttgttt agtttaacca ccgcaagcga ctcttcacgt
     2281 gaacctttag gatttcgcaa accaatacgc caagcaccat ccgaacgtct tggtgattca
     2341 ccaaaaacaa cgatatttcc tcctaagtta actaaagcaa acgctgcatt tcgctctcgg
     2401 caatgtgtaa ttaatttatc tgcaatgtaa ccttttgcta acgcccccaa gtcaatcatc
     2461 attcctggtt tggttaaaaa aacggttttc attttttcat ctaattgaat ttggtgtgga
     2521 tccgttagca gaagtaactc agttatctct gcttttgttg gaaccaatgc atcttcaaat
     2581 ccaatgtgcc atttttgtac aaccggacca atagcaatat tcagataact atttcttgca
     2641 caactatgtg cttttcctaa tttgatgagc tcaaaaagtt caggatgtac aatcacagct
     2701 tgtcgtccag cagcttgatt gatggccatt aattcagaag ttgcatcatg tgcgctgaag
     2761 cgatgttcat aaacgtgcaa ccattcgcct aactctgcta gtaatcgact aggctcttca
     2821 tgcccaactt gtaatgtaat tgttgtcccc atcatttgcc agattttcgt ttcttcgatc
     2881 acctgattca ttttgtacct tctttacttg aactacggat tacatgagta attagttttt
     2941 atttttgtta ctgttttcac aaccagatga tacgcctgct agatttattt gtcaaccgat
     3001 tttatatatt tttttatata ataaggtaat tttaatataa aattttatat tgtaaaaaaa
     3061 caaataaggg aaacaaacaa cacatttttg tgtgttgctt atttccctct aactatcgtt
     3121 ttctatttga tggttaacct acttaaaata aatactgcaa cgacaaacgg agatcaagca
     3181 tgctttgtaa cgtattttac gataattacg actttatttt attactctat cttgctgcca
     3241 tacccgtcta tccctaatca tctttggtga attttttcaa agttagcata acaaaaataa
     3301 gtattttttc cttagttaat caatagtatc atttttttca tatgatttaa ttcatcaatt
     3361 aattttttgt ttttattaat tttagcaaaa gctagtgcat cttgaatcaa ttcgagcact
     3421 tctgccttcg acttattttt agcctgtgcg ttcttcgcta attgggcagc agctcgtgct
     3481 aagtaataag tcaaatggtt atccgaacaa atatcgattg cgtaatttag tagtgcatca
     3541 cttgtttcca aatcaccgtc ttgcgcaaaa aaaacaccac aatgaaagac gatatttaac
     3601 acccgccaga tatcttctgt tgtacgaatc ggatacgtat aaattttttc aaacactcga
     3661 ttgaaataaa cttctgcttt ttccaactct ttttctctag catagaccat tccaatacct
     3721 gtatatgcca ataaacgaaa caacgtgttg gctgtttctg ccatcaacaa ctgatcaaaa
     3781 tggaacaaca catccgtaat tggtgcattc gtaaaaatca tttcaaatcc ttttagatag
     3841 tagtaacgtg caatcagctc agactcgtca attaccttgg gatcaatggc tgctaaaatc
     3901 tgtgtcaatt gttcatattc actcgtaatc agtgaaaact ctgctttttc tagctgttca
     3961 tcaagttgtg tattgatcac gttcatcgaa gaaaaaagtt caactaacgg aagattgatt
     4021 cgattacata atttgattaa aatttttaac gaagggactt gaccattgtt ctcaaattta
     4081 cttaatgttg cttgtgtaca aattccttca gacacttctt tttgagataa gttggcagcc
     4141 tttcttgccg taataaattt ttcaatgttc attattttct ctccaatcag acctcacacg
     4201 aaaatcattt ctgtctcgat tttagcatgt tttctttggg aatgctttag gttctgttgc
     4261 aaagttttaa ataaagaata aagtccctta cggtatctat gatttaagct
//
LOCUS       DAZABD010000126         4211 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000126 DAZABD010000000
VERSION     DAZABD010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4211
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.132"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(573..950)
                     /locus_tag="ACHALG_002802"
     CDS             complement(573..950)
                     /locus_tag="ACHALG_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFN2588104.1"
                     /translation="MKKSYPVIFSAEENGYSVVFPDFAGATQGDNFEEAMSNAKEFLD
                     GILAYYIDEDISVPSPTDIHQLNNLGNNKIVSLIQSDPSPFIQKKTVRKNVTVPEWLV
                     ARGEKDKINFSKVLTDALLKIYE"
     gene            complement(996..1184)
                     /locus_tag="ACHALG_002803"
     CDS             complement(996..1184)
                     /locus_tag="ACHALG_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFN2588105.1"
                     /translation="MPLTGKKMLKLAKKNGWKVVRITGSHHIVAKEGFPPVPIPVHSN
                     QDLKKGTEQKLLKDLGLK"
     gene            1376..1897
                     /locus_tag="ACHALG_002804"
     CDS             1376..1897
                     /locus_tag="ACHALG_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN2588106.1"
                     /translation="MNKKQDQEYYIEKLEKENLELKERIRYYETKFCKRGDCMKPNPV
                     ETGKRIKSIRSNFGMTMEQFADLIGNAQKGNINNWERGFSLPNKERLIKIAELSNKTI
                     DWIKWGTLEEYITSYLIGIGYELYIKDFPEIPHKVFKDIQEKYSDTFSLDKDYELLDS
                     IIKNIFTKYYSNG"
     gene            2092..2988
                     /locus_tag="ACHALG_002805"
     CDS             2092..2988
                     /locus_tag="ACHALG_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN2588107.1"
                     /translation="MEINLFEAGNRIKQIREKHGYTMADLGKLVDANSPSTINNWEKG
                     NNLPNRKRLEKIALLGGTSVEWIKYGDFSDYVYRLTQNIEEKILKEYGETTLNNYREA
                     LLDELTIEKITYEEDTKILAAAEKINTQNIFQEVKYMYSPDEDYRNIEVKPDLGLTDD
                     NKNIKMIGQRIRKIRKKENITLEEFGKLFSPTADKAVISNWENGKNLPNSERIKKIAE
                     IGGVSELYLMTGVDSSVAESMYFLSEVALDALERLSIEEVNRIIESFAGYLNVISKID
                     DPDKREISLNAICQLSLLDKMG"
     gene            3037..3411
                     /locus_tag="ACHALG_002806"
                     /pseudo
     CDS             3037..3411
                     /locus_tag="ACHALG_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353455.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            3691..>4211
                     /locus_tag="ACHALG_002807"
     CDS             3691..>4211
                     /locus_tag="ACHALG_002807"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN2588108.1"
                     /translation="MTQTDFAELMGLSRSYIGDLENNRSNPSIKTLEAIARALDVPIV
                     ALINDSSTDNIVEPYESLTAENILLKQENEELKYEVESLRKRLYEIQQVVNQGAEKMI
                     PNLKDVSKRIKYIRKKLGMTMEQFGSHLSNSPKSTIATWESGRNIPSQKKLQLIAIVG
                     ETTTDWIKWGTLE"
BASE COUNT         1576 a          612 c          770 g         1253 t
ORIGIN      
        1 taaaagtgag tagaataaga aatcgttgat ttggcaacga aatagtgagg ggacactcac
       61 tcactgaagg gttagctctg tcaagttgtg taaccagacg gaaggacgaa tattgttgag
      121 gttttagaac catgtaaaac aacacagttc tcaaatgtta cggataaacc gtttgcggga
      181 aatcaggttt taaaaccatg taaaacaaca cagttctcaa ataaggcttt attgaattga
      241 tcagtagttt ctgttttaga gttatataaa acgacatggc tcttaaatag gagctaacga
      301 agaaaaagaa ggtactccgt tttggagtta agtagaaata aaaaaataga atcgttgatt
      361 tggcaacgag ttgaaaaata cgcaaaacta tggtcaccaa gggataaccc tgaaactgat
      421 gatcgctata tcttactagt agttaaacag tctaaatagt actcatttat tgtattgatt
      481 ctaagggaac ctgcaataaa tgagtactat tttactttta attccgctat ctatgtcatt
      541 aggcaactaa attgttatta atatatttac gtttactcgt atatttttag taacgcatct
      601 gtcaaaactt tagaaaaatt tattttatct ttttcccctc tagctactaa ccattctggg
      661 actgtgacgt ttttgcgtac ggtctttttt tggataaatg gagaaggatc actttgaata
      721 agagaaacaa ttttgttatt gcccaaatta tttagttgat gaatatctgt tggagaagga
      781 actgaaatat cttcatcaat atagtaagct agaatcccat caagaaattc tttagcattg
      841 gacatagctt cttcaaaatt atctccttgt gttgctccag caaaatcagg aaaaactact
      901 gagtagccat tttcttcagc agaaaaaatt acagggtaac tttttttcat ttccaccact
      961 cctttcgagt taatccaatg aactaaagga ctgggctatt tcagccctaa gtcctttagt
     1021 aacttctgct ctgtcccttt ttttaaatcc tgattactat gaacaggaat aggaacgggt
     1081 gggaatcctt ctttggctac aatatgatgg ctgccagtta tcctcaccac tttccaacca
     1141 tttttcttgg ctagtttgag cattttttta ccggtaagtg gcattcgcga ctacctcctt
     1201 acaaatatta ttatacgcat tatgcgtata aaggtcaaga gtttttacat taaaagtaga
     1261 atgtaagtgt cacaataggg tataccctat tgtatcatat ggtaaagtac agtataataa
     1321 ggtttggagg tgtaacacta gggtgtaaca caatgtgaaa tttggaggtc attttttgaa
     1381 caagaaacag gatcaagaat attatataga aaaattggag aaagagaact tagaactcaa
     1441 agagcgaata agatactatg aaaccaaatt ctgtaaaaga ggtgattgta tgaaaccaaa
     1501 tcctgttgaa acaggtaaaa gaattaaatc aattcgttct aattttggaa tgactatgga
     1561 acaatttgca gatttaattg gaaatgctca aaaaggaaat attaataatt gggaacgagg
     1621 attctccctt cccaacaaag aaagattaat taaaattgct gaattaagta acaaaactat
     1681 cgactggatt aaatggggaa ctttagaaga atatataact tcatacctta tcggtatagg
     1741 atatgagcta tatattaaag actttccaga gatacctcac aaagtcttta aagacattca
     1801 agaaaaatat agtgatacgt tttcgttaga taaagactat gaattattgg attctataat
     1861 aaaaaatata tttacaaaat attactcaaa cggttgaatt gatagatgga ctcaaagaag
     1921 aaaattcaaa gttaaaagag aagataaaat actatgaatg tttatttgat ggtatagaga
     1981 gtctgctgaa ctcacgaaaa aatccctagt ttttatgaat gaccgttttg taaatctgat
     2041 tgaaatagat ttatatgatt tcggtatatg atatacatta ggaggtgtcg aatggaaata
     2101 aatctttttg aggctggtaa tcgaataaag caaatacgag aaaaacatgg atatactatg
     2161 gcagacttag gtaaactggt tgatgccaat agtcctagca caattaacaa ttgggaaaag
     2221 ggaaataact tacctaatag aaagcgtctt gaaaaaattg cactattagg tggtacttct
     2281 gttgaatgga taaagtatgg agatttttcc gattatgttt atcgcttgac acaaaatata
     2341 gaggaaaaaa ttcttaaaga gtacggagag actacactga acaactaccg tgaagctctt
     2401 ctagatgaac ttacgattga aaaaatcaca tacgaagaag atacaaagat attagcagct
     2461 gccgaaaaaa tcaatacaca aaatattttt caggaggtga agtacatgta ttctcctgat
     2521 gaagattatc gaaatataga agtcaaacca gatttaggac tgactgatga caacaaaaat
     2581 attaaaatga ttggacaacg tataaggaaa ataagaaaaa aagagaatat tacattagaa
     2641 gaattcggca aactattctc tcctacagct gataaagctg ttataagcaa ctgggaaaat
     2701 ggtaaaaacc ttcctaactc agaaagaatt aaaaagattg cagaaattgg cggagtttct
     2761 gaactttatc ttatgacggg tgtcgattca agcgtagcag aatcaatgta ttttctatct
     2821 gaagtagcat tggatgcttt ggaacgtctg tcaattgagg aagtcaatag aataattgaa
     2881 agcttcgccg gttatcttaa tgtgatttca aaaattgatg atccagataa gcgagaaata
     2941 tctctcaatg ctatttgtca actatcactt ctcgataaaa tgggttgatt cacaaaaaat
     3001 attgaaagat tactttactc acgaggaggc ttagctatga aacctaatct atcgttaact
     3061 agtgaacgta ttagaaaaat tatcgaatcc aaaggtctga cattagaacg attcggtgat
     3121 ttaattggcg acacaccaat aagttctgtt aataattggc ttagaggcgt gtctttaccc
     3181 aataaaaata ggcttttaac aattgcaaat attggaaaca ctagtgtaga ctggattaaa
     3241 tggggaactt tggaagaata catagcttca taccttatag attcaggtta tgagctatat
     3301 attaaagact ttccagagat accttacaaa gtctttaaag acattcaaga aaaatatagt
     3361 gatacgtttt cactagataa agactatgag ttattgaatc ctataataaa atttactata
     3421 gggggcgtta ctttgaatag aataaaattt tatattgatt ccagtaaaaa gaccattaaa
     3481 caaattagta cagaaactgg tattaactat acaactctta cgaactatta ccgtggtgag
     3541 cgaaatccta gaaatactga aacatggaat atccttgctg attatttcca tgttcctgta
     3601 agttatttga tgggattaga cgaaacaaat gttgaactca aaaaccttaa tattggaaac
     3661 aacatcaaaa atattagaaa aagtaaaaat atgacacaaa ctgattttgc tgagttaatg
     3721 ggtctatcac gatcatatat aggggattta gaaaacaaca ggagtaatcc cagtataaaa
     3781 acactggaag ctattgcaag agcattggac gttccgatag tagcattaat caacgattct
     3841 tctactgata atatagtaga gccgtatgaa agtttgacag ccgaaaatat ccttcttaaa
     3901 caagaaaatg aggaattaaa atatgaggta gaaagcttac gaaaaagatt gtatgaaatc
     3961 caacaagtag taaatcaagg agctgaaaaa atgattccca atctaaaaga cgtaagtaaa
     4021 agaatcaaat atatcagaaa aaaattaggt atgactatgg agcaatttgg ttctcattta
     4081 agcaactcac ctaaaagtac aattgctact tgggaaagtg gtagaaatat accttctcag
     4141 aaaaagttac agctgattgc tatagtagga gaaaccacca ctgactggat taaatgggga
     4201 actttagaag a
//
LOCUS       DAZABD010000127         4155 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000127 DAZABD010000000
VERSION     DAZABD010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4155
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.133"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            177..773
                     /locus_tag="ACHALG_002808"
     CDS             177..773
                     /locus_tag="ACHALG_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFN2588109.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            897..2696
                     /locus_tag="ACHALG_002809"
     CDS             897..2696
                     /locus_tag="ACHALG_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HFN2588110.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(2974..3186)
                     /locus_tag="ACHALG_002810"
     CDS             complement(2974..3186)
                     /locus_tag="ACHALG_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2588111.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            3648..3797
                     /locus_tag="ACHALG_002811"
     CDS             3648..3797
                     /locus_tag="ACHALG_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2588112.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            complement(4050..>4155)
                     /locus_tag="ACHALG_002812"
     CDS             complement(4050..>4155)
                     /locus_tag="ACHALG_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFN2588113.1"
                     /translation="ASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
BASE COUNT         1229 a          707 c          728 g         1491 t
ORIGIN      
        1 taaaaaaaac aagttattaa ataagtcttt agttggatgt ttttttgaga tcaatctttt
       61 atacttatga tgaacttaca aaattgtttg ttttctgtca tatcaaaaaa tggcgcagtt
      121 agttgctttt ctagtaaagt atagttaaca aagaacctct aaggagttga tgaataatga
      181 atattgctgc ctctcgaaaa ctgataaatt tcatatccat tatcggatta ggcctatcaa
      241 tcgcgctgac gatttatttt attaatttag gtgttttcaa agatttgaat gcgttaagag
      301 gattagtagg tgattccatc attttaggac cgattatttt tgtcttcatc caaatcctgc
      361 aagtagttat tccaatcatt cctggaggga tcagtactgc agctggtgtc ctgatatttg
      421 gcccttatgc tggatttatc tataactata ttggaatctg cattggttcg attatcattt
      481 ttctgctagg gcgtcgttat ggaaagccat tcatactttc catgattagc gataagacat
      541 ataacaaata tattggctgg ctggataatc aaaaccgttt tgagaagctg tttgctctag
      601 ctatttttct tccagttgct cctgacgatg cgttatgttt gatggctgga ttgacaaaca
      661 tgtcagtgaa acgatataca ctaatcattt tgattgctaa accactgtcg attttcttat
      721 atagtatggc cttgatttat ggaggtcaat atctaagtgg tcttcttgga taatatttat
      781 tttgtaaaat tagtcattga ttgacaatgt taaatctggg aaagggtctg caaaacacac
      841 tgcacccttt cctttttcat tagatttgcg ctacaatgtg aagagcaggt gataggatga
      901 attatttgaa acgtagctta ggtaatgctt gggacttttt aaaaggaaca caagcttatt
      961 tccgtgatgt cttgctgatg catggcttta ttttatttat ttgtttaccc ttattgagca
     1021 gtacgacccg ttttatttta caccgagggg caattgacta tttgtcttat gacaatattc
     1081 cttcaatttt ttctcaacat cctggggttc tactctcgtt aatcgtagtt ttgatgacga
     1141 ttttactgtt agtttatttc gaatttactt ttcttttgat gagtgttttt tttattaaaa
     1201 agaaagaacc aatctctttg aagcaactct tacacttgac gatacttcag ctgaagaaag
     1261 tgagacccat tacttttttg tttttccttg cttatttttt attgattcta ccgatcagcg
     1321 gattaagttt taattctgat ctactgtcaa aaataaaaat ccccgctttc attatggatt
     1381 ttatttttgc taatcgttgg atcatcgtca gttccttttt actcgtttat attttccttg
     1441 gctatattgg gattcgactg atttttgctt taccggaaat gattctacga gatcgtcctt
     1501 ttcgagcagc cattcgggaa agctggtcat tgaccaaatc gcgtcttttg gccatcacgg
     1561 gccaatttat tgtgattggc ggtacgattt tactcctttc ttctttagga tatatcgttg
     1621 ttattttggc acaaagtatg gttgaacaat ttttcccaga ctatgcattg atcagcgctg
     1681 tgtttgctat gacgctttta caagggattt tattattcaa tatcgtgatg tctaccgtag
     1741 ggatttttta tatcattgtt gatttcatgg atgacgaagg cttcttgcct gagatcccta
     1801 agtggtttat cccacaagcc cccaacttgc gtttctctgc gctgaaaaac actggtttga
     1861 ctctttttgc agtttttttc ggtattggcg tctgtcttta caatatggat tatctgactt
     1921 cagctgttca gacaaaacca gtgactgtct ctcacagagg tgtgagtacc caaaatgggg
     1981 cgcaaaatac tttagaagca cttgaaaaaa caagcagaga ctatcatcct gattatgtag
     2041 agatggatgt tcaggaaaca aaagatggtc atttcgtagt gatgcatgat gcaaatcttc
     2101 gtcacttaac aggggtaaat ggaacaccac aggatcttac attgaaagaa ttgacaaatc
     2161 ttacagttac tgaaaacgga gtgcaagccc ctgtttgttc ttttgattct tatttggaaa
     2221 aagcacataa gctaaaccaa aaattactta ttgagatcaa gacctctaaa aaagattcgg
     2281 atgaccttgt cgaacgattt gtccataaat accgcaaagt tatcttagag aaccatcata
     2341 ttttgcaaag tttgacgtac caaaccgttt cagatttaaa agaagcagaa ccaaactttt
     2401 atgtgggcta catcttacct ttcaatattg ttgggccccc tgtgacgcct gctgattttc
     2461 tgacaatgga atatagtacg atcaaccgta acttcattaa ttctgcccac caagacggaa
     2521 aagaagtata tgtatggaca atcaacgatt cagatgctat gagtcgcatg atgttttatg
     2581 gtgtggatgg tatcataacc gatgagatgg gactactaaa tgaaagtgtg aaacaaacag
     2641 atgaagaaat cacttattca gataaactgc taaattttgt tttgggagtc ggctaatcaa
     2701 aaatcgttaa tgaaaaaagt ctttatacaa caaaaaacgc ataacctgcc accgcaggta
     2761 tatgcgtttt ttgtttgacg ctcataatgt gtgcttaact ttctcttgat tccttcatcg
     2821 cacgaacaat agctaaaata gcaggaattg cagagatcac tgcaagaata attcccattt
     2881 tccctgaact ttttcttttt ggcatatctt ctcgctccct tctgtcttta tttatattat
     2941 aagggagttc ttgcgaattc tcaaaaatac tgtttaagaa accaattcag caggtgcgct
     3001 aatatcacag aattttacga gcatgcgata cttgctttca ttgataagtt gatagtctcg
     3061 ttggtcataa ctttcaacat acacgacagc cgtgttgata tatgtacgtt caatacagcc
     3121 gattagttca tgagatactc cgatcggttt gcatatataa gatcttccta cttctgcttt
     3181 taccatttca ttccctcttt tctttatttt tattttgttt tttctttatt ttataataac
     3241 aaattacctt gtatttgaaa acgtttttat ccattttttt attttttatt ttgagtatct
     3301 ttctgctttt tagataacta ttttttaaca cgatcttaga cgttgtgaca aaaacgtttg
     3361 tttatattcc tatgttgttc gtgcgttttt ttctttatat tgctaagtta tttataaaga
     3421 ggtgagcata tgatttatat tcaagcaata gtatttgtaa ccttgatgat tgggctactt
     3481 atcggattaa aagaaaatag ctaaaccatt taagaaaaaa taaataagga aaatgataaa
     3541 tgctttttaa ttgcatcaaa aaacgtacga tgctaaactt ggtataagtt cgaaagttta
     3601 aaggaaactt tcaagaacta aattacatta caaaggagat gaattttatg acgtttacaa
     3661 aaacaaatat gcaataccgc gtagcttttg atacaacttc taaccgtttt atggcgattg
     3721 atgcacaaaa cgaaaataac gttgcgtttg gtattacaat cgaacaagca gtccgagcat
     3781 taaacgaatc tagataagga gtgaaccatt ccaaatggcg actgatgaga tctcggaaaa
     3841 tcaataaaag acgtttgtaa cggtaatccg cgacaaacgt ctttttgtat tcattacata
     3901 tatttggaaa ttgtctctct ttatctctag acctaaacaa aaacacaagt tctaatatca
     3961 accatctcgt tttttatgac aattttgtta gctgtttcga tgataatatt ccgttattat
     4021 ctgtataata tttaatataa tcttaattgt caaattaagt tagacagatc atcaatactt
     4081 acgtaactca aaaatacttc caaaggtgtt cgataatcca gtgattttct aggaatatta
     4141 tttcgtttag atgcg
//
LOCUS       DAZABD010000128         4092 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000128 DAZABD010000000
VERSION     DAZABD010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4092
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.134"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..153)
                     /locus_tag="ACHALG_002813"
     CDS             complement(<1..153)
                     /locus_tag="ACHALG_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFN2588114.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWV"
     gene            complement(475..696)
                     /locus_tag="ACHALG_002814"
     CDS             complement(475..696)
                     /locus_tag="ACHALG_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2588115.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(794..1039)
                     /locus_tag="ACHALG_002815"
     CDS             complement(794..1039)
                     /locus_tag="ACHALG_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2588116.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1279..1893
                     /locus_tag="ACHALG_002816"
                     /pseudo
     CDS             1279..1893
                     /locus_tag="ACHALG_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2263..3264)
                     /locus_tag="ACHALG_002817"
     CDS             complement(2263..3264)
                     /locus_tag="ACHALG_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HFN2588117.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFDPIEFDNDERL
                     LLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRSYLSDRVFDVLEKLSKSGILKEEYD
                     SSFDEYPSRYILISEDYKKFKRYFLKNEKKISVIMEKYKAPIQADPFSNLDESDLPF"
     gene            complement(3423..3689)
                     /locus_tag="ACHALG_002818"
     CDS             complement(3423..3689)
                     /locus_tag="ACHALG_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN2588118.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(3679..4035)
                     /locus_tag="ACHALG_002819"
     CDS             complement(3679..4035)
                     /locus_tag="ACHALG_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFN2588119.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT         1295 a          744 c          542 g         1511 t
ORIGIN      
        1 tacccaacga taaatcgtag tatgacaaac atttattcca cgatcatata acaattcctg
       61 aacttcacga tagcttagat tgtaacgcag gtagtaacca acagcgacaa taatgacgtc
      121 ttttttgaat tgtttgcctt taaaatgatt cattactctg tcctctctgt cttttttctc
      181 aattttacac taaaatagat tttttggaaa actttgcaac agaaccgttt ttactatata
      241 cttcttccca gaacttgtcc gtctccttca aattatctct acctctcgag ttcattgggt
      301 ggtttgccca tttattaaac atggaatcac ctttctcata ctcatatatt tatgtcctat
      361 ttcaattata tttatatatg cttaaataaa tttgtatcct gaaatttatt gtaatatgaa
      421 gctgtatttt acatatagta accaaagagt taataaaata aaaagagcga accattatag
      481 atcagctctt ttttcattgc tgtttttaat cttatctaga atcacttcaa atgtttcaaa
      541 ggtatttaca tctaactgac ataaccaaag caagtacttc ttttctttgt aagtccaatc
      601 aatattacgg taagaggaca ttagattatg gtaccttgct ctttgttcaa ctgaagccaa
      661 ttcccagcgt ttttcaaata ttttttcttc ttccattatt gatccctcgc aagacgctac
      721 ctcaaactta aataatgggc agcgaaagtc taattcctta gatctaagta tagcaaacaa
      781 atatgaaaaa aacttagaag gtagtaaaag ttcttccgtc ttttgctttg cttctattct
      841 taatgtgttt cgttatcggt gccctacaaa gattttcatt aattacacac tcatttattg
      901 ttgtgtattt tctgacattt ctctcttgat caattatttt aattccatgg gcatcctgat
      961 agttcatagg agaggagcgg ctatcagaaa tagttgcacc tatctcttga acttctttta
     1021 caacttctgg aaaatccatc catcctgctt cagctgtcaa aagtaataat ctataatatt
     1081 tatctttcct tttcaatcta agaaatcgtc accccattct ttttcttcta ttataaacta
     1141 tttaactttt agaattcaaa aaccgtttgt ctatttagag tttaccctaa atagacaaaa
     1201 atataacatt gaaaaattta gagttctaac gtatgattag ggtgtattat aaacgttata
     1261 gaaaagtagg gaataaattt gaaaattgct tatattagag tttcatccat cgatcaaaat
     1321 gaacaacgtc aaattgaaga aatgaaaaag tttggcgctg aaaaaatttt tatcgaaaaa
     1381 caatctggag cgacgattag tcatcggccg attttccagg aagctttaga ttttgtcaga
     1441 gagcaagata tttttattgt agaagccatt gatcgacttg gacgcaatta tgacgagatt
     1501 atcgattcag tgaattatct aaagaagaag aatgtgcgac ttattatcac cagtttaccg
     1561 atcatggctg aagcaattgg aaatcctctc ctagataaat tcatcaaaga cttaatcatt
     1621 taaatctcag ccatgattgc tgaacaagaa cgtactgaat cgaaacgacg ccaggcacaa
     1681 ggaattaaaa tcgctaaagc aaacggcgtc tataaaggtc gtcctaagtt atacagtgca
     1741 gacaccaaag atcctcaacg tcgccttgtc tataggagta tcgttcagga tcttgagaat
     1801 ggggttgcta tctcaaagat tgcaacagac tacaatgtga ctaggcaaac aatttaccga
     1861 ataaaaaaag aaatcgacca actaattgta tgatttcttt tttgaatcac gtaattatta
     1921 attagctttg tcatcaatac agacaatcat tttagattca tctagctaag ttgattacat
     1981 ttatgttaaa aagaatttag ttaacataaa tataattgat gcataaatcg tccttattta
     2041 aaggcattta acattgacaa cttagtataa tgtatcttat gtaaactaat aagatttgaa
     2101 ttttatttta tacacactcg ataatagatc aattttatgg tcccccgcat ttacgattgc
     2161 acaagagaaa cataaagtcg acctattagc atttcttttc aattcaaaca ttaaaaaagt
     2221 ttatacctct gctactaaaa aatgaaatta tctatttaaa atttaaaacg gtaaatctga
     2281 ttcatccaaa ttggaaaatg gatcagcctg tattggagct ttgtatttct ccataataac
     2341 agatatcttt ttttcatttt ttaaaaaata ccttttaaat ttcttatagt cctctgaaat
     2401 caaaatatag cgtgatggat actcatcaaa agaagaatcg tattcttcct ttaaaattcc
     2461 cgatttactt aatttttcta gtacatcaaa tactctatca ctcaagtaac tccttaaatt
     2521 gttatcactt tcccattgaa ctacttttct ataagttgaa ttatcagttg aaaagataga
     2581 attttcatcc ataaacgcat atattagcag caatctttca tcattgtcaa attctattgg
     2641 atcaaaaata tctaagttgt catcaaaatc tttatcagtt ccggtaactt ttttgttaat
     2701 tactgaaggt gtcttgtaag aaatagaatc taaatcaaag cgttttgatt ctaaattatt
     2761 ttgtgctttc tcaattaaat aattcacttt ttttgtgaga aaccttctga tattgtcttt
     2821 gctatatgtt ccataaaaac ccttatcttc taaagagtcc ctgtacttct taacttctat
     2881 atactcttca gccaaaactt gtggaactgg ttcatctaag aaaaaaaccc ctacctcgcc
     2941 tttgctattt ttataaaagt aatcaatttc tgctatagtt cccgatctat aattatcaac
     3001 tggagaaccc aacttatggc caaaaatcgc taataatata tcactatttt ctacaatttg
     3061 tttattaata acactttgcc cagagctatt atttatataa gtaggaatcg cattcgtttc
     3121 ccatcgaatt ggcatcaaaa ttacttttgt attatctgta ttttctatat tccatctata
     3181 tatggtttct tctacttcat tcctctttgc tgctatatca cctggtgatg ctataaatac
     3241 ttttattaga tttatggttt tcatttattg tacctttctc tttatgtctc tttaaagtaa
     3301 tttttgtata aaatttattg acttttacac tataggacaa cttttatcta tttagtacaa
     3361 aaaggcccac taaactaatt gattagttag gagcctatta caggtatatt ttcaactcgc
     3421 ttttaagcaa attctactac cagctttttg cctaatgccc tagcgatatc atcaagttta
     3481 tcgaaagata cgttatcccc tctctcaatt cgagcaatag tagattgagt tgtatgtgct
     3541 ttttgtgcaa gctccgcttg agttaggcct gcttcctccc gtgcatgata aagagcaacc
     3601 gcagtttcga gttttcttcc ttcacggtca tatgcttctg caaaatcttt gtctttttgc
     3661 ttttcttcaa tcaatttttt aatcatcatt tgtgaaccac tcctctctaa gttcttttgc
     3721 atgtttaagt tcacttattg gtgttttctc cgttttttta gtaaatccat gggtaatgac
     3781 ataccttcca ttgaccacat ggaaatagat agctctttgg atgtttgatc ctaccttact
     3841 tcttaattcg tacaaattag tgtctaattt tttcacccat ttcaaccgtt gagctactag
     3901 catcccattc ttttctgttt cttctattgt acgtaaaagc ttcgcactat ctttttttgg
     3961 taatgtttcc aaccattcta aaaattcatc atgaccatta ggtcttttaa atgtttcaaa
     4021 agatattttt tccataccca attatatcat atattgattt tatattatag tatataacat
     4081 aaaaattcga at
//
LOCUS       DAZABD010000129         4026 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000129 DAZABD010000000
VERSION     DAZABD010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4026
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.135"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(19..540)
                     /locus_tag="ACHALG_002820"
     CDS             complement(19..540)
                     /locus_tag="ACHALG_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFN2588120.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            789..2411
                     /locus_tag="ACHALG_002821"
     CDS             789..2411
                     /locus_tag="ACHALG_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFN2588121.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            complement(2540..3403)
                     /locus_tag="ACHALG_002822"
     CDS             complement(2540..3403)
                     /locus_tag="ACHALG_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFN2588122.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
BASE COUNT         1306 a          766 c          730 g         1224 t
ORIGIN      
        1 ttatttgttt gattttactt atctaaatgc atagacgata tgtgtgatac caaataacat
       61 aaaatagaat ccaactaaaa agcttaatgt caacgcagaa gaaattggat taaacaataa
      121 gaaaattcct aataaaattc ctaaaatatt gacgatgatc gtaaaccaat aatatccttc
      181 acttactcct cttgccaagt cagcagtaaa taacgctaaa actgaatcag caataaacca
      241 tactgcaaaa acaaaaggca atgctgcgac tcctgcacca atattgaata agaaaaagac
      301 gccaactaga atatcgatga tcccgattat taagggcatc tttcctttat atcctgttag
      361 ctcttttaat ctgttccgta caaacagttc aaatatccct ttaataaagg caaaaatcgc
      421 aaagactact acaattgcaa ctaaatttcc gacagggtct tgaaatgata acaatgagac
      481 aagtacaaat aaaattccca aaatcaatga gccccagtcg atggttcttt tttcatccat
      541 tttcaatcct tctttcattt cttttacagt tttatagtac tccctcttat aaataacgtc
      601 aaaaattatg ctgttgcaaa ctaataaatc ctatagtagc tattttcgct ccgcttttat
      661 ttttattttt ttcattctgt ttcacgtgaa acacaaagaa tgttcgattg ccttgaatct
      721 tttacctttc ccctatataa ttctataaga cttgtcaaaa ctaataatga taaaagtgag
      781 gaaaaaaatt gattactgta aatgatgtga gtttgcactt ttcagaccgc aaactatttg
      841 atgatgtgaa tattaaattt acccccggaa actgctatgg cttgattggt gcaaatggtg
      901 caggtaaatc aacgttcctt aaagtgttat caggggatct gcagccttcc acaggttccg
      961 ttactatggg accagatgaa cggatggccg tattgaaaca gaaccattac gactatgaag
     1021 actatactgt acttgaaaca gtcatcatgg gacacaagcg tctgtatgaa gtgctgaaag
     1081 aaaaagatgc gatttacatg aaagaggatt tcactgacga agatggtatt cgagcagctg
     1141 aattagaagg agaatttgct gagcttaatg gatgggaagc tgaaccagaa gcagctgttt
     1201 tactgcaagg tctgaacatc cctgaagaat tgcaccatca gcaaatgagt gaattaactg
     1261 gtggacaaaa ggtaaaagtg ttattagcac aaacactatt tggtaaacct gatgttcttt
     1321 tgctagacga accaacaaac ggtctggata tccaatcgat caattggctg gaagaatttt
     1381 tgatcaattt tgaaaatact gtgatcgttg tatcccatga ccgtcacttt ttgaacaaag
     1441 tatgtacaca tatggcggat ctagattttg gtaaaatcaa actttatgta ggaaattatg
     1501 atttttggtt ggaatctagt caactagcgg ctaaacttca agcaaacgca aatgccaaaa
     1561 aagaagaaca aatcaaagaa ctccaagaat ttattgcccg ctttagtgcg aatgcatcaa
     1621 aatcaaaaca agcaacttca cgtaaaaaaa tgcttgaaaa aattacttta gacgatatcc
     1681 aaccttcttc tagacgttat ccattcgttg gattcaaacc tgaacgtgag attggaaacg
     1741 acttattgca ggtagaaaat gtaagtgtaa cgattgatgg aaagaaaatt ttagatgata
     1801 tatcttttaa tttgagcaaa gaagacaagg tcgctttcgt cgcagaaaat gatataacaa
     1861 caacaacgct attcaaagtt atcatgggtg aaattacgcc agatactgga acagtacgtt
     1921 ggggtgtgac aacaagtaat tcttatttac caaaggatac aacgaaagaa ttcgatactg
     1981 atctaacgat tttagactgg ttgcgtcaat atgcaagtaa agaagaagat gataacacct
     2041 tcttaagaag cttcttagga agaatgttgt tttctggtga agaagtattg aaaccagtga
     2101 atgttttatc tggtggagag aaagtccgtt gcatgctttc aaaaatgatg ctttcgaaag
     2161 ctaacgtact tgtattagat gatccaacga accatttaga cttagaatca atcacagcat
     2221 tgaatgacgg cttgatggca tttactggct caatcttatt tgcgtcacat gaccatcaat
     2281 ttatccaaac attggcaaac cgcatcattg ccgtttcgga taaaggtgtg atcgatcgag
     2341 ctgatacgac atatgatgaa tttttggaaa atgcagatat tcaaaaacaa ttgaacgagt
     2401 tatgggcata atggcctatt tagcttaaaa aataaaaaaa cagcgacctc taagagatag
     2461 gatgctcatc tatcttttaa gagtcgctgt ttttgttaca atcaatttct gggctttttt
     2521 acagaatgag ttactgcttt tatcgactga ttcccatcga aaaaatcgcg atacagccat
     2581 agcctgtgtg actggcgatc gttggtccta agggataaag cttcacatct ttcatcttta
     2641 tctgctcttc caaaagccgt tttgccttct ccgcgctctc tagatcccca gcgtaagcca
     2701 cataaacgat ttgatcaagt ggtgcaacga ttgtttttac cgtctcattg acgattgtct
     2761 ggatcgactt gtttcttcct cgaactttgt caacattcgt cagccttcct tctttatcca
     2821 ccgtaataat aggctttaca ctcaatagac tgccaagagt agcacttgct ttggaaatcc
     2881 taccgcctcg ttctagatat ttcagatcgt ctactgttac ccatgaatgg actttcaaat
     2941 aattactttc cagccactct agggtttctt cgatgctttt accttctttt ttcaatctta
     3001 cagcttccaa tactaaaagc ccttccccaa tacttgcagc ttttgtatca aaaacataga
     3061 tctcagggtc ttcatattct tccttcagca attcaaccgc ttgaagcgca ctactgtaag
     3121 agccgcttaa tccactggaa aaagccacat ataaaacagg aatctgtttt tccgcataag
     3181 aacggaaaaa ttctaaatat cggccaacat tgatttgtga tgtcgtaggc atcgcaccat
     3241 ttttcagctg attcatgtag cggtcataat tgaatgtctt tccgaggtca tctactaact
     3301 cttgatcatt caattggaga ttcattgata aaaaatcaac tttttctttt gctaatacat
     3361 ggtatggcag atcacaacaa gaatctgtta caatttgata cacatttttc aaccccttct
     3421 cgttacattt tacccttatc gtctattata gcaaatctct tgcgctagac acactttaac
     3481 cggtttattt tcgcaagttc taatcaaaat aagaggttgt gacagaagtg gtcagcttca
     3541 agaaataaga aggaatttac gaaaattgct tctcaaattt ttgtgaaatt tcggcttatt
     3601 tccgtagaag ctacttctgg aacaccgtga atcagaggtt gtgaaatcag cgttttgacc
     3661 tgagtattag agaaaaaaac ggaaaaaata gttcctcata ttttgccgtt ttttgagcta
     3721 ataccgaggg tcagctgatt gaacaccgtt taccaagagg ttgtgagcct gccgtttagc
     3781 tccgagtcac aaggaacaat ttcaaaaaac agcttctcat gtttgttgaa tattgtgact
     3841 tgtgaccgag gagttggcag gagaacaccg tttagtggag aggttgtgaa aaagctgctc
     3901 tgcatcaagc agtaagaaca atcagataaa aatagcactc catatttttc atctgattgg
     3961 gcttagtgcc gtagatgcaa gcttttgaac accgtttgtt cggaaaaagg gactgtgaca
     4021 cggttt
//
LOCUS       DAZABD010000130         4011 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000130 DAZABD010000000
VERSION     DAZABD010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4011
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.136"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            200..661
                     /locus_tag="ACHALG_002823"
     CDS             200..661
                     /locus_tag="ACHALG_002823"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2588123.1"
                     /translation="MKLEEEKDAIYTQMRMILEERRRLSKDYYELKARLFALDNTNQP
                     NSLIRNEKLAKKSSTNISEEALYQQYICERGNKYRQLIPYSTISLTISSILKDAGRPL
                     SNKEIYKLLISDHKLNISYKNLTHNILPKMNVDNSVNVERAYRGYWQYRLH"
     gene            722..1108
                     /locus_tag="ACHALG_002824"
     CDS             722..1108
                     /locus_tag="ACHALG_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFN2588124.1"
                     /translation="MSENDERVNAQLICFFEKVIKNTAYSYFERQKKIRELEQFDQFS
                     QHFFTENNIKIKQPVTILDVTFLIEDSRLAEIIPLLKEREQLFLIEKFVFDKTDKEIG
                     ESFGITRQGITNLKHRLYRKIQKLLI"
     gene            1182..1373
                     /locus_tag="ACHALG_002825"
     CDS             1182..1373
                     /locus_tag="ACHALG_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN2588125.1"
                     /translation="MNTLKDDFILAKAGSEEAIESILKRFSSLMHQQSWRNGRYDQDC
                     YQECMIAVYLAISKFEIKE"
     gene            1921..2880
                     /gene="bioB"
                     /locus_tag="ACHALG_002826"
     CDS             1921..2880
                     /gene="bioB"
                     /locus_tag="ACHALG_002826"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFN2588126.1"
                     /translation="MMNTINTKMTKSDCLEILSSKENIWFYYSQALYTKTKNMKNRIR
                     LNTLLNAKSGLCNEDCGYCAQSKKSKATIQKYSLLPQDEIIRKALIAKKNNSSVFCIA
                     LSGTRPTQKEVLVLGETIKEIKRIMDIEICLSIGLITDNQIRYLKKCGIDRLNHNLNT
                     SRENYPNITTTHSYDDRLNTLKKIKAFDINICSGFICGMGETDTQLVDLAFELKEQEP
                     FSIPVNFLLPIKGTKLEKYNELTPIKCLKILIMLRLLFPKTEIRISAGREFHLGSLQQ
                     FAILIVDSIFLGNYLTEKGADVTEDYQLLKELELIVEGDYNEY"
     gene            2870..3535
                     /gene="bioD"
                     /locus_tag="ACHALG_002827"
     CDS             2870..3535
                     /gene="bioD"
                     /locus_tag="ACHALG_002827"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFN2588127.1"
                     /translation="MNISMVICGIDTNCGKSVASILIYEYLKLYFSVNPIIVKPVQTG
                     QELDTIFYQQCDIPQKNIFNFYSLDIEASLHTAGNLTNQKIDLQSLLDQSKALLSDNR
                     PKIFELAGGILSPINDKETMLDFVKLLNLPIILVVDNYLGSINHTLLTIHLLESKNIE
                     ILGLIYNNTESRNYSTELFEYITNNTCIPQIGKIPYLKNLKYVLKSEKEKQNLIREWH
                     LNV"
     gene            3528..>4011
                     /locus_tag="ACHALG_002828"
     CDS             3528..>4011
                     /locus_tag="ACHALG_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010709177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HFN2588128.1"
                     /translation="MYRKSQMIARMSLMLALLIITARLVIPLPLFDYLSLQIITVYLI
                     YPTLGMKNGLIVTCSYLILGIIGLPIFASGGGYAYIFKPTFGFLLAFVTLPIIQSFIS
                     NKFNNAIYSSFKKNAIINICCLLYIYLIGIIYKILILSFYTNNVTSLSILISITTLID
                     F"
BASE COUNT         1514 a          550 c          567 g         1380 t
ORIGIN      
        1 tataaaaatc aaaaagagac gaaataatcg ttcattttat gaaaacaaag aaaataaact
       61 tgcttcgtca tatactatta atagtatatc agtttagaaa aaaattcaat atgagcgctc
      121 atttttaatt atgtaataat atactatttt gtgtcctttt aaaaatattt ttagttgtta
      181 atcatgaaag gagaagtaga tgaaactaga agaagaaaaa gatgcaattt atacacaaat
      241 gagaatgata ctcgaagaaa ggaggagact tagtaaagat tattatgagc taaaggccag
      301 actttttgca ttagataata ctaatcaacc taatagttta attagaaatg aaaaattggc
      361 caaaaaatcg tcaacaaata tttctgaaga agcgctgtat cagcaatata tttgcgagcg
      421 aggaaataaa tatagacaat tgattccata ctcaactatc agtttgacaa tttcctcaat
      481 tctaaaagat gctggtcgac cattatcaaa taaagaaata tataaactat taataagcga
      541 tcacaaactt aatatttctt acaaaaactt aactcataat attctaccca aaatgaacgt
      601 tgacaatagt gtcaatgtag aaagagctta tagaggttat tggcagtata gattgcatta
      661 gcttcttttt tcgctttctg tcccttttcc ttttaaatat ttattggaag aagtgagcag
      721 aatgtcagaa aatgatgaac gcgtgaatgc acagcttatt tgtttttttg aaaaggttat
      781 taaaaatact gcgtactctt attttgaaag gcaaaaaaag attcgtgaac tggaacaatt
      841 cgatcaattt tctcaacatt tttttactga aaacaatatc aaaatcaaac aaccagttac
      901 tattttagac gttacttttc taattgaaga ttcaagattg gcagagatta ttcctttatt
      961 aaaagaaaga gaacagctat ttcttattga aaaatttgtt ttcgataaaa cagataagga
     1021 aatcggagaa tcttttggga tcactagaca aggcatcaca aacttaaaac atcgtcttta
     1081 cagaaaaatt caaaagttac ttatttaaaa gccacttaac gattatttgt atacgaccac
     1141 ctcctctaaa taactttaaa tttaggaaag gaggacggtc tatgaatact ttgaaagatg
     1201 acttcatttt ggcaaaagct ggaagtgaag aagctattga gtctattctt aaaagattta
     1261 gttctttgat gcatcaacaa tcatggcgaa atggaagata tgatcaagat tgctatcaag
     1321 aatgtatgat tgcagtatat cttgcaatat caaaatttga aatcaaagaa taaaatcaag
     1381 ggagaggcct tttgcttctc tctttcttat cgattttaaa aaaataatat taaagttttt
     1441 tattaaacta tttacttcct tttacagtta tttgaaaaat gcttcttcct ttctttacta
     1501 gtaaaaaata ttttttcttc cttaaatttc caaaattatt ctaacagcaa atgtacttcg
     1561 ttttaagtat gctttattaa atactatttg ccttttaaaa tagcggataa atatgattaa
     1621 ttagggagaa aaagggatat attcatctcc tagaactctt aactaaatgg ccttttagta
     1681 ggtagcaatt gataaaaact aatatattga atgctagtaa ctcttgataa agataaaact
     1741 aacaaaattt ttttaattaa agaattagca gctttccaaa tgttttcatt taactatgtc
     1801 cctaaactga taagcttatc ctttattaat aaaatttaaa cattatcagt aatttacccc
     1861 ttcattttcg ttaacctgta taatataatt ggttaaccaa ctacaaggag aattactatc
     1921 atgatgaata ctattaatac aaaaatgaca aaatctgact gtttagaaat tttaagttca
     1981 aaagaaaata tttggtttta ctattcgcaa gccctatata ctaaaactaa aaatatgaaa
     2041 aatagaataa ggttaaatac acttctaaat gctaaaagtg gattatgtaa tgaagattgt
     2101 ggttactgcg cccaatctaa aaaaagtaag gcgaccattc aaaaatacag tcttttaccc
     2161 caagatgaga ttatacgtaa agcattgata gcaaaaaaga ataattcaag tgttttttgt
     2221 attgcgttaa gtggaactag acctactcaa aaagaagtat tagttctcgg cgaaacaatt
     2281 aaagaaatta agcgcattat ggatatcgag atttgtttat ctattggatt aatcacagat
     2341 aatcagataa gatacttaaa aaaatgcgga attgatagat taaatcataa tttaaatact
     2401 agtagggaaa actatcctaa tataactact actcattcct atgacgatcg tttaaatacg
     2461 ttaaaaaaaa tcaaagcttt tgacataaat atctgctctg gctttatatg tggaatggga
     2521 gaaactgata cacaattagt cgatttagct tttgaattaa aagaacaaga gcctttttca
     2581 attcctgtaa attttttatt acccattaaa ggaaccaaac ttgaaaaata taatgaacta
     2641 accccaataa aatgtctaaa aattctaata atgcttagac tattgtttcc aaaaacagaa
     2701 attagaataa gtgctggtag agaatttcat ttaggcagtc ttcagcaatt tgctatttta
     2761 attgtagatt ctatattttt aggaaactat ttaacagaaa aaggcgcgga tgtcacagaa
     2821 gattatcaat tattaaaaga gttagaactt atcgtagagg gggattataa tgaatattag
     2881 tatggtgatt tgtggaatag atacaaattg tggtaaatca gtagccagta ttttaattta
     2941 tgaatatctg aaactctatt tttcagttaa tccaattatt gtaaagccag tgcaaacagg
     3001 acaagagctt gatactattt tttatcaaca gtgtgatata cctcaaaaaa acatttttaa
     3061 tttctattca ttagatatcg aagcctcact acatactgca gggaatttaa caaatcaaaa
     3121 aattgactta caatccttgt tagatcaatc aaaagcttta ctaagtgaca atcgacctaa
     3181 aatatttgaa ttagctggtg gaattttatc tcctatcaac gataaagaaa caatgttaga
     3241 ttttgtcaaa ttattaaacc ttccaattat cttagttgta gataattact taggctcaat
     3301 taatcatact ttactaacta tccatctttt agaaagcaag aatatagaaa ttttgggatt
     3361 gatttacaat aatacagaat ctagaaatta ttctactgaa ttgtttgaat atattactaa
     3421 taatacttgt attcctcaga ttggtaaaat tccttatcta aaaaatttaa agtatgtttt
     3481 gaaatcagaa aaagaaaaac aaaatttaat tagggagtgg catttaaatg tatagaaaga
     3541 gtcaaatgat tgcgcgaatg tcattaatgt tggcgctgtt aattattaca gctagacttg
     3601 ttattccttt acctttattt gattatttat ctttacagat tattactgtt tatctcattt
     3661 atcctacatt aggtatgaaa aacggtctga tcgtaacctg tagctactta attctaggaa
     3721 taataggtct tccgatattt gcaagcggtg gtggatatgc atatattttc aaaccaactt
     3781 ttggtttctt gctagccttt gtgactttac ctattataca atcgttcatc tcaaataaat
     3841 ttaataatgc tatctactct tcttttaaaa aaaatgctat tataaatatt tgttgcctac
     3901 tttatattta tttaattgga attatttata aaatattaat cttgtctttc tacactaata
     3961 atgtaaccag tctttctatt ctgatcagta ttactacatt aatagatttt t
//
LOCUS       DAZABD010000131         3721 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000131 DAZABD010000000
VERSION     DAZABD010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3721
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.137"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..883
                     /locus_tag="ACHALG_002829"
     CDS             <1..883
                     /locus_tag="ACHALG_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HFN2588129.1"
                     /translation="ISVPVALFSFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTY
                     NDYFQIGMIGMIVSLVVVLTVANISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVL
                     GVVLLDLQIILGFILAGIWALLFTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMT
                     LAMFNNSAAYASPYFSAIFGDWIPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILA
                     VVLAVNPTMSPAFLFPLFAITTIAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGW
                     IIGAILCLITFLLYGNA"
     gene            931..3060
                     /locus_tag="ACHALG_002830"
     CDS             931..3060
                     /locus_tag="ACHALG_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HFN2588130.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKNLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKAIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPHTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            3035..>3721
                     /locus_tag="ACHALG_002831"
     CDS             3035..>3721
                     /locus_tag="ACHALG_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2588131.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKEIE
                     KRCTEYDLDYK"
BASE COUNT         1175 a          665 c          837 g         1044 t
ORIGIN      
        1 tatttcagta ccggttgcac tgttctcatt tatgggctcg attatggctg gaatctttgc
       61 taatatcgtc aatttcaaac aatatcaaac gatttatgct ggtttcaatc cagcggctga
      121 aagctatact tataatgact atttccaaat tggtatgatt gggatgattg taagcttagt
      181 tgtggtacta acggtcgcta atatatcaat gaacaaaaag aaacgctatg caatggcagc
      241 aaacgtacca gctgaaggtg gcgacgcacc aatgatctca tggttagccg tattattacc
      301 agttttaggg gtcgttcttt tagatcttca aatcatctta ggttttattc tagcaggtat
      361 ctgggcattg ctgtttactg gaaaattgcg cggaggttat aaagaaatct gtcgtcagtt
      421 tgcgaaatta tttacagatg gcgcagtcga tgttgcacca atggttggtt tcttgatgac
      481 cttagcgatg ttcaataatt cagctgcgta tgcttctcct tatttttcag ctatttttgg
      541 agattggata ccgcaatcac cattagtctt ggcaatcgta tttgctatat tgacaccact
      601 aggatttttc cgcggtccaa tgaatctagt cggttcaggc tcagctattt tagctgtcgt
      661 gttagctgtt aatccaacga tgtcgccagc ctttttgttc ccgttatttg caattacaac
      721 gattgctcct cagcatttag atattacaca atcttgggtt gcttggggat taggttatac
      781 aaaagtaact tctcgagaat atatgaagaa atccatccca actggttgga tcatcggtgc
      841 gatcttatgc ctgatcacgt tcttattgta cgggaacgcc tagtaaacaa aacgtcgata
      901 aaaacgatta gataaatgga ggaaaagaaa atgaaacgtt ttgtgcgaat gggaattgat
      961 gttggcggta cacatacgaa agcagtcgca atcgataatg cgacgcacga aatcatcgga
     1021 aaatcttcag tgaaaacaac ccacgatgat gtccgtggag tagctgcagg tgtcgttcag
     1081 tcttttcaaa attgtttgcg ggaaaataat atcagtccag aagatgttgt gtttgttgca
     1141 cattctacca cacaagcgac taatgcactg attgaaggag acgtagcaaa agtcggcgta
     1201 atcgggatgg caaaaggtgg attagaaggt tttttagcaa aacgacaaac tagattgaac
     1261 gatatcgatt tagggaataa gaaaaaaata gagattgtca atgcattctt acctgtcaaa
     1321 aacttaaatg tagaccgtgt ttcagaaacg atttcttctt tagaaagaga aagagcggag
     1381 gtcttggttt cttctatggc atttggagta gataatgggg aaccagaacg agttgtttat
     1441 gaagcagcat ctgtgaaagc gatccctaca acgatggctt ctgacatcac aaaattatat
     1501 ggattgacaa gaagaactcg gacagcagca atcaacgcat ctattttacc aaaaatgcta
     1561 gatacggcaa cctcgacaga agactccgta cgagaagcag gggtcaatgt ttctttgatg
     1621 attatgcgcg gagacggcgg agtaatggaa atcaatgaaa tgaaaaaacg acctgtttta
     1681 accatgcttt ccggtcctgc tgcatcagtc atgggctctt tgatgtactt gagagcgtct
     1741 aatggtgttt attttgaagt aggcggaaca acaaccaata tcggggtcat taaaaatgga
     1801 cgtccagcaa tcgattattc aattgtaggt ggccacccaa catatatctc ttcattagat
     1861 gttcgggttc taggtgtggc aggcggttcg atggtacggg ctaatcagtc aggaatcatc
     1921 gatgtcggtc cacgttctgc acatattgcc ggtctagatt atgcagtatt tacggagaca
     1981 gaaaaaatca aaggtcctaa ggttgaattt ttctctccta aagaaggaga ccctgctgat
     2041 tacgtcaagg tagtgatgga agacggagaa gaagtgacta ttacaaatac atgtgcagcc
     2101 aatgttcttg gattagtgca agaagaacat ttttcttatg gaaatgtccc atctgcacga
     2161 aaagccatac aggcattagc agactattgt catacgacgg ttgaagacat cgcagagcag
     2221 atcatggaaa aatcctatgc taagattgaa ccggtgattt tagaattggc agacaagtat
     2281 cacttggaga aagatcagat ttctcttgta ggtgtagggg gcggcgctgc ctcattgatt
     2341 acttacttta gtaataaaat gggtgtgaaa tattcgattc ctgaaaacgc cgaagtgatt
     2401 tcttctattg gtgtagcact tgcaatggtc cgagatgttg tagaacgtat catcccttcg
     2461 ccaagtaaag aagatatacg atcgttgaaa aatgaagcga tgaataaagc gattgaatca
     2521 ggcgctacac cagaatcgat cgaagtccac gtagaaatcg atccacatac ttcaaaagta
     2581 acggctatcg ctactggttc tacggaagtg aaagcaaccg atctaaccaa ggagatcaca
     2641 acagaagaag cactagagct tgctgcagag gatatgcgat taaataaaaa tgaagtttgc
     2701 ttattggaaa atacaccttt cttttatgta tgcggcgaac agaatcgttc aaaaaatgct
     2761 ggaagtcttc ggatcatcga tcaaaaagga tttatcaagg tccaacgtgg acatgcttca
     2821 tgtatgaaaa caactgcagc caattatatg acagctgtcg aacaactttg ggaagatatg
     2881 gcagtttatc aaactgaatt gattgcccga ccagaatttt atctctgctt aggagcaaga
     2941 gtctctgatt ttacagcgac ggatctggaa caactacagc ttttgatgga tctggaagta
     3001 tctactatgg aaccggagga agaagtgatc gtcgttgccg gaaatatcaa acaaacataa
     3061 gttagaagag ttactaaacg aattacaaca gatccccgaa gaaatttggg gattctacca
     3121 gtttcggcga gatttatttt ggaaaaagat tcctttgtcc aagcagaaaa ttcttataca
     3181 acaatcaatc gattgtggta tcgaaacggc atgtagcatc aaaaaaaagt acccttttgc
     3241 cgatgtagga gaaatctgtg aacaaatggc tgttccaatc gtttcctgtg aatcagagca
     3301 aataaacgaa cgaatcactt ttgcaactta tgcagaagat gaagggatcc gattgatgac
     3361 ggagccgtta gaaaaattaa aatgttcagg actagcttcg atctctaaag aaacagcgca
     3421 agctttgatt attggacatg aactatttca ccacatcgaa gcttctgtta agggaattta
     3481 tacgcaaagc gaaaaaattg tgctgtggcg gttgcctttt tatacacatc aatcaaacat
     3541 tcgtgcattg agcgagatag cagcgatgag tttttcaaaa gagatgaatc aaagcgattt
     3601 ttcgccttat gtattagagg cagtcttact ttggccatat aatgaaattc gtagccaagg
     3661 gatcttagaa gaaataaaag aaatagaaaa aagatgtacg gagtatgatt tagattacaa
     3721 a
//
LOCUS       DAZABD010000132         3709 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000132 DAZABD010000000
VERSION     DAZABD010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3709
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.138"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            960..1928
                     /locus_tag="ACHALG_002832"
     CDS             960..1928
                     /locus_tag="ACHALG_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFN2588132.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKSDIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELL
                     QQLEKLEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
     gene            1945..2469
                     /locus_tag="ACHALG_002833"
     CDS             1945..2469
                     /locus_tag="ACHALG_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFN2588133.1"
                     /translation="MTEKTIKELAEELGVSKTAINKKVSDENRKLWFSKIGNKFVINE
                     TGQKAIKSMFFNENENSNQQLVGEKTKTENHKNANQVPELEVLSLLEQQLNQKDMQLQ
                     EKDEQIKQMQKLLDHQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIE
                     KQSKAEKKWWQFWQ"
     gene            2845..3126
                     /locus_tag="ACHALG_002834"
     CDS             2845..3126
                     /locus_tag="ACHALG_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000353845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2588134.1"
                     /translation="MDILVRNINPKIIARIDKRCQELTTKTGKKWSRNKYLKVLIESD
                     FDRQLMNYKKDQFDLLLEKFIAIQTENTNILNDYVHTNNQLIETLIGGI"
     gene            3128..3544
                     /locus_tag="ACHALG_002835"
     CDS             3128..3544
                     /locus_tag="ACHALG_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2588135.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
BASE COUNT         1390 a          655 c          619 g         1045 t
ORIGIN      
        1 cactttagtg agttttgtag aaagtacctt taaaggtact ttcacattac gaacgatacg
       61 tccgtaggac gtactgaaag ggtgggattt atgagcgaac taatgcaaaa tttggctagc
      121 aaaaaaagtt aaaaaatata tgctaataat cgagcgaaat gaacactttt tcaagtgagc
      181 gaatccgcca tgacaaagct gaagcagttt atttcagctt tgtcatggcg tgtgcctaga
      241 cttttaaaaa agtcggcaca cgagcaaata tctttatcaa gctctaattt gcgttttaag
      301 acgtttgaaa cgtctaagta tataaatata taagagtagg tttaagagtg cttaaatcga
      361 attttagagg gcattcagac aactttaggg ttgttagggc ttgccctgac cgcctgtaag
      421 gcgctttaag aacaagataa gggatttata ctaagactgt cttaaaacgt ctcaaacgaa
      481 ggctcatagc ttctcaaaga gttccttatg aacattttta gcaaaggagc cggaagcgac
      541 tgcgcagtat aatgtgagag cagagcgatc acaccttttt aagcgactgt agggagtaag
      601 attcccagtt gagaaaaatg cgcgaatctg tttggagaaa aaagaagtgg ttttttaggc
      661 gggaaatttt gtccccctct tttataatct ctttttaaac ctctttttaa acctctttac
      721 cgtcggctat atccttactc tcccaaggga atgacgcttt tatctacatc gttttgtctg
      781 tttatctaca tcgttttgtc tgtttatcta catcgttttg tctgtttatc tacatcgttt
      841 tgtctgtttg tacattgaat tagtacatcg acgatgttaa tataagatgt agataaacag
      901 gcattttgta tttcataacg tgtatctctt gtgcgagtaa gaaaggagct attttaaata
      961 tgtctaatga aatcgtaaag tatcatcatg aattaaatac aattccgttg cgaaaattta
     1021 caccagtcga aatgaatttg ttcttttcta tcgtatcaag aatgcgtgat gtaggtgata
     1081 aaaaagtaca atttacattc gagcaattaa aagatttaag caattataaa gcaactgcaa
     1141 atgttcgatt catagatgat ttagaaacaa cgtatgacaa attaatggat ttacgttttg
     1201 gtagaagaag tgctgacggt ttgcaacgtg aacgttttgt tttatttaat caatttaaaa
     1261 tcgacggaaa atctgatatt ccttttgctg aaatacaagt tcatgaaaaa gccttaccct
     1321 tacttaataa tcttgaagaa tgggtgcgct attctctcca acaattcaat gaattagaaa
     1381 gcagttattc aaaaacaatg tttcgattac taaaacaata cagaaccaaa ggtttcgctt
     1441 atttttcaaa agaagacttt catgaattac ttgatattcc taaaagttac aaccaaggta
     1501 cgattgatca acgtgtgttc aatccaatac gacaagaact aaccgcaatc tttaaaggtt
     1561 taacaatcaa aaagaaatac ggaaaaggac ggggaaagcc tgtcattggc tatcaattta
     1621 catttaaacc tgagataaaa aaatcagacg actttaacaa acatgcccca acaaaagaag
     1681 aacaaaacaa agcattagaa ctagctaacc aacaagttca atctgaaaaa gcacctactt
     1741 cattacaaga aaaaatcaaa gaacaaaagc gaaaattgga acttttacaa cagttagaaa
     1801 agttagaaag agaacaaaat acccaaaata aagaaaataa tgcacaaaaa tcagagataa
     1861 attccgatat tccttcagaa ctaaaaaaag acttattaga caatcttcaa aacttattca
     1921 aagattgagg ggtttttaga ttttatgaca gaaaaaacaa tcaaagaact tgctgaagaa
     1981 ttaggagtta gtaaaacagc aattaacaaa aaagtttctg acgaaaatcg aaaactttgg
     2041 ttttcgaaaa tcggaaacaa gtttgtgatt aatgaaaccg gacaaaaggc tataaaatca
     2101 atgtttttta acgaaaacga aaactcaaat caacaactag ttggcgaaaa aacgaaaacc
     2161 gaaaaccaca aaaacgccaa ccaagttccc gaattagaag tcctttctct cctagaacaa
     2221 cagttaaatc aaaaagatat gcaactacaa gaaaaagacg agcaaataaa acaaatgcaa
     2281 aaattactcg accaccaaca acaactcacc ctgcaagcca accaacaaat cgagaaactg
     2341 caggaacaac tacagttgac ttatacggaa gaatcttcgg aaaataaatc aacgactctt
     2401 tctgaagaaa cagaatcgat agaaaaacaa tcaaaggcgg agaaaaaatg gtggcaattc
     2461 tggcaataaa accagcaatt taaattaata aaaattgctg gttttattct tctttttgct
     2521 tttgcgagca gagcaacatg gacccatgtt gcgatatttt ggtggcgccc caatccccgt
     2581 tttttcgttg aatcttactc ggactgcact cgttttacag ctgatttttc tgaattttga
     2641 aagagcataa ggaaacgtgg tatcactttc ttacgaaagg tgctagatag gctgtgccta
     2701 tcacgtttct cagaaaaaaa tttgctatca cttttttgcc attgtggcta ggctatgtat
     2761 agtgttttgg cgaaaaatag ctatacaaat actatatttg tatggtatac ataaattata
     2821 cgctgatacg aaaggaaaat ttccatggac attttagttc gaaatatcaa tcccaaaatt
     2881 attgcaagaa tagataaacg ttgtcaggaa ctcacaacca aaacgggcaa aaaatggagt
     2941 cgaaataaat atctaaaagt tctaatcgaa agtgattttg accgtcaact tatgaattac
     3001 aaaaaagatc aattcgattt attactcgaa aaatttattg ccattcaaac agaaaatacg
     3061 aacatactga atgactatgt tcacacaaac aaccaactca ttgaaacact tatcggagga
     3121 atataacatg agaaaaactt ctgttcaaaa acacgtaacc ctttatccca gtcacgttga
     3181 cgttttagat tccatcatcg aaaaaaatcc agacgtcaac aattattcag aagccattcg
     3241 ttttttatgt atgagttatg aacctgatac caatacaacc acaaccagta accaagcaaa
     3301 actcaatgcc atgggaaaag aaatttctat catgacagaa atcatggcag aatttgctga
     3361 tttatatcta aaagatactg gtgttcttat cggtacaaat tctaaagtgt atcaagaagt
     3421 gaaagaaaaa attgaaaatc aactcaatcc ttcccgacga aatcaaccca aaaatcgtca
     3481 aaaagaaacc aatcaaacct ttacttctgg aaattttgac ccaacaaact ttctaaaaag
     3541 ataaaaaaag aaaccgatcc ctaaaaaaat tcctcgattt ctttttaggg atcagtcttc
     3601 cagcaagatt tgcaagttgg taccaacttg cgaaaaaaaa gaaaaatccg cttatgcaac
     3661 ttcttctttt tgtattttgg ggcgctaccc caatccccaa gtatcggtt
//
LOCUS       DAZABD010000133         3680 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000133 DAZABD010000000
VERSION     DAZABD010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3680
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.139"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..276
                     /locus_tag="ACHALG_002836"
     CDS             <1..276
                     /locus_tag="ACHALG_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HFN2588136.1"
                     /translation="ELIKLANKGQDYEHLADEIDELRDKRQLLLVEDASLSGENERIN
                     ELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEEHFVISFKSGIEMEI"
     gene            419..640
                     /locus_tag="ACHALG_002837"
     CDS             419..640
                     /locus_tag="ACHALG_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN2588137.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGNEIKIGN"
     gene            656..1498
                     /locus_tag="ACHALG_002838"
     CDS             656..1498
                     /locus_tag="ACHALG_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2588138.1"
                     /translation="MKIITINDVEYAVFAANEGTSKPQPHIIETKSGTIPEGKQLSLL
                     KEYLKQNDISPIKGATTYWCIDKVLKLDSSKEKTISETIHKQKYLSLTEENIEKQHKF
                     VGASSNYGKEGLIIHDVLNAFPLHNDLNTIAMKIAVIDVTNSTHLSQYKSRLSLYDLA
                     KVILEIPNFDDRLAKGDPQLINIIARNIGAVNMFSFASKYCTYHNVEVCGRDDYSIFD
                     GIVKNTLPHYIQGLTTNKIDTWRRSFDYEAFNECVGKLLDENNIHIPFRRRKLDHFLW
                     YANR"
     gene            1561..3363
                     /locus_tag="ACHALG_002839"
     CDS             1561..3363
                     /locus_tag="ACHALG_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HFN2588139.1"
                     /translation="MGLFDFLKKKQGKVSKHVDYQLASSLFRTGKVFAEKLEVDISNV
                     DEFRKTFIAFDVETTGLNSSSDRIVEIGAVLFKEGVPIQSFGTLVNPKVRIAASASAI
                     NNITNEMLNSAPNEQEVYPQLVEFLGDALDGKITMCAHNASFDFGFLSNTFSRLGYDA
                     KIKYVDTLSLSRKHIKGIVNHKQETVAEYFGLINDTAHRAESDADICGKIFWGVLDII
                     EESLEEQKKQIEKMTPDIKELEACAFIQDIIEKKGCDTSWIRYRKNSNNYVDVTCLYT
                     FLKFKFAKKGNYIIIRKDATNGIDLPIEPCTVSEGGTTNVRVYFRSPFDLTPLSNYIF
                     SAYSDCYNSMNEYMSVSNYTKKEAEQSLSMLKSISKSAMKELLFDAKNRGYDETEVNL
                     NIEKVISREDVIINGLHNRVSLNKIRNIGNWEKGFDAGYPYWERGETARKNGKVDEAI
                     ALFDKARYNGYDAPALYDSYAKAYRKIKDYDNEILILDEGIMRKTRHDVGTLEARRDK
                     AIKLLFAKQEAERIAKEKSDFLKANKKEDAISEPSKKRGRQIIQMTDDGTIIKEFDTI
                     ASAVKEVGVNSKSIRDAANGVQKHAGGYYWKYKD"
     gene            complement(3422..>3680)
                     /locus_tag="ACHALG_002840"
     CDS             complement(3422..>3680)
                     /locus_tag="ACHALG_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HFN2588140.1"
                     /translation="GITISIPPRKNMDKSEKLDHSLLGKQRKTVGTVFSSLEKLGCQN
                     FNSRSVKGLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT         1311 a          522 c          750 g         1097 t
ORIGIN      
        1 gaactcatca agctggctaa taagggtcaa gattatgaac atctagcaga tgagattgat
       61 gagctgagag acaagcgaca gctcctttta gtagaagatg cctccctcag tggcgagaac
      121 gagcgaatca atgagctgat tgaatttatc cgcaagaaca aattccgtac cttagagtac
      181 gatgataagc ttgtaaggaa gataatccag agcgttacag tctatgaaga acacttcgtc
      241 atatccttca aatctggcat cgaaatggaa atatgaaaac cagaagtaaa tagcccatga
      301 ttctacagta gagtcgtggg ttttcttttg cttgctgatt ataataatat tttaataact
      361 attgttatta taaaccaaat gatttataat attcttagcg taaacttgga ggtgctgtat
      421 gactacggca gaaatgatta aagaactgtg tgagcaaatg aatataagtg tttccgaact
      481 tgctagacgt attggacaga ctccacagaa tttcaataag aaattacaac gagaaacggt
      541 aaccttagat gagttgaaag cgattgctga tgtgctaggt gtcaagtttg tgcaggcttt
      601 tatattgcct gatggcaatg aaataaagat aggtaattag gaggtgtaat ttgtattgaa
      661 aatcataaca ataaatgatg ttgagtatgc tgtatttgct gctaatgaag gtacttccaa
      721 accacaacct catatcatag aaacgaaatc tggtactatt ccagaaggaa aacaactgag
      781 tttattaaaa gaatatctta agcagaatga tatttcccca ataaaaggag caacaacata
      841 ttggtgcatt gacaaggttc ttaagcttga ttcaagtaaa gaaaaaacta taagtgaaac
      901 aatccataaa caaaaatact tatctttaac tgaagaaaat attgagaaac agcataagtt
      961 tgtaggtgca agctctaatt atgggaaaga aggtctaata atacatgatg ttttgaatgc
     1021 atttcctctt cataatgatt taaatactat tgcgatgaag attgcggtaa ttgatgtaac
     1081 aaattcaaca catctttctc aatataagag caggttgtct ctatatgatt tggctaaagt
     1141 aattctggag attccaaatt ttgatgatcg gttggcaaaa ggagatcctc agttgataaa
     1201 tattatcgca aggaacattg gtgcagttaa tatgttctca tttgcatcca agtactgtac
     1261 atatcataat gtggaagtgt gtgggagaga tgattactcc atctttgatg gtatcgttaa
     1321 aaatactctt ccacactata tccaaggact aactacgaat aagattgata cgtggcgaag
     1381 aagttttgat tacgaggcat ttaacgaatg tgttggaaaa ctacttgatg aaaataatat
     1441 acatattcct tttagaagaa gaaagttaga tcacttcttg tggtatgcga ataggtagaa
     1501 tataagaaaa aggcatcgta aaagtgattc tggataaaat taatggggga ggaaataata
     1561 gtgggattat ttgattttct aaagaaaaaa caaggaaagg ttagtaaaca cgttgattat
     1621 caactggctt ctagtctatt tcgtactggt aaagtttttg ctgaaaaatt ggaagtggac
     1681 atatcaaatg tagatgagtt tcgtaaaact tttatcgctt ttgatgtgga aacaactggc
     1741 ctaaattctt cttcagatag aattgtagag attggtgcgg tgctatttaa agaaggagtt
     1801 ccaattcaat cattcggaac tcttgtcaat ccaaaagtta gaattgcagc ctcagcttcg
     1861 gcaataaata atataactaa tgaaatgcta aactctgctc caaatgagca ggaagtttac
     1921 ccccagttgg tagaattttt aggagatgca ctagatggaa aaatcactat gtgtgcacac
     1981 aatgcaagtt tcgattttgg atttttaagt aacacatttt ctagacttgg gtatgatgct
     2041 aaaattaagt atgttgatac tctgagtctc tctagaaaac atataaaagg cattgtaaat
     2101 cataagcaag agactgtagc ggaatatttc ggattaataa atgacactgc ccatagagcg
     2161 gaatcagatg cagatatatg cggaaagatt ttttggggtg ttttagatat tattgaggag
     2221 tcgttggaag aacaaaaaaa gcaaatagaa aagatgactc cagacataaa agaacttgaa
     2281 gcttgtgctt ttattcaaga tattattgag aaaaaaggat gcgacaccag ttggatcagg
     2341 tatcgaaaaa acagtaataa ttatgtcgat gtaacttgtc tatatacatt cttaaagttt
     2401 aagtttgcta agaagggaaa ttacatcatt attagaaaag atgctacaaa tggaattgat
     2461 ttacctatag aaccttgtac tgtaagtgaa ggcggtacaa caaatgtgcg agtctatttt
     2521 cgtagcccct ttgacttaac gcctctttca aattatattt tcagtgctta tagcgattgt
     2581 tataattcta tgaatgaata tatgagtgtt agtaattaca ctaaaaaaga agcagagcaa
     2641 agccttagta tgctgaaatc tatttcaaaa tcagcgatga aagagttatt attcgatgca
     2701 aaaaatcgtg gatatgatga gactgaagtt aacctaaata ttgaaaaagt gatatctaga
     2761 gaagatgtaa ttattaacgg gttgcataat cgagtttcat taaacaagat acgaaatata
     2821 gggaattggg agaaaggatt tgatgcaggt tatccatatt gggaacgtgg agaaactgct
     2881 aggaaaaatg gcaaagtaga tgaagctatt gcactttttg ataaagctcg atataacggc
     2941 tatgatgctc ctgcgctata tgattcttat gctaaagctt atcgaaaaat taaggattac
     3001 gataatgaaa ttctcattct tgatgaagga attatgcgta aaacacggca cgatgttggt
     3061 actttagagg ctcgtaggga taaggcaatt aagctattat ttgctaaaca agaggcagag
     3121 cggatagcta aggaaaaatc tgattttcta aaagcgaaca aaaaagagga tgcgattagt
     3181 gagcctagca agaagcgtgg aagacagatt attcaaatga ctgatgatgg aacaataata
     3241 aaagaattcg atactatagc ttctgctgta aaagaagttg gcgttaactc aaaaagtatt
     3301 agggatgctg caaatggagt tcaaaaacat gctggaggat actactggaa atacaaagat
     3361 tagcagataa tctaagataa cctaggatct tcttaataga tacccgtggt gctagttaat
     3421 tttaatatcc taaagaatat ctcaaagttc cttcaaaacg tcgttgtgct cgacttaata
     3481 gaacactgta agccagtaat atgctttcaa atctactctc tagcccttta acagaacgtg
     3541 agttgaaatt ttgacaacct aatttttcta aggaagaaaa aacagtccca actgtttttc
     3601 tttgttttcc aagtaagctg tggtctagtt tttctgattt atccatgttt ttacgtggcg
     3661 gaatggatat agtaatgccc
//
LOCUS       DAZABD010000134         3542 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000134 DAZABD010000000
VERSION     DAZABD010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3542
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.140"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..342
                     /locus_tag="ACHALG_002841"
     CDS             <1..342
                     /locus_tag="ACHALG_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HFN2588141.1"
                     /translation="SWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGLHDQ
                     LEEKNEENMFYKIVQSFENETAKKLLIQFYEEQATDRLQEYVEKLIKNEEISEMIRKR
                     LLLEAEKAVKE"
     gene            339..2658
                     /locus_tag="ACHALG_002842"
                     /pseudo
     CDS             339..2658
                     /locus_tag="ACHALG_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
     gene            2719..3261
                     /locus_tag="ACHALG_002843"
     CDS             2719..3261
                     /locus_tag="ACHALG_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2588142.1"
                     /translation="MEDILIATFITALGAFLTIFVVTIKGFKDTDSLQKDHQSILDGQ
                     KTHTESIIVHLSEGFTYLKDAMPTFTVKLTEIFHSVEQISSELKEQRQQKDRQLGSLN
                     FRQETIQESLENVNRLNDEMQRVHLENEELRAQNYDLIQKKRNLEQKIVELKEENRQL
                     NYQLRRGKNRSRDLGNELEL"
     gene            3339..>3542
                     /locus_tag="ACHALG_002844"
     CDS             3339..>3542
                     /locus_tag="ACHALG_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2588143.1"
                     /translation="MDKYIHMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKIVNNLV
                     HLPLFFALLNYGLWLAIALVIVWR"
BASE COUNT         1299 a          514 c          708 g         1021 t
ORIGIN      
        1 tcgtgggata tcaatgatat agacatacat tgtacacatg cggacttgga tcttaaggct
       61 ttacaaattg attatgatta tttcctacca gatgaatatg ctagaaatct ctatcatgga
      121 ctgcatgatc aattagaaga aaaaaacgaa gaaaatatgt tttataaaat cgttcaatct
      181 tttgagaatg agacggctaa aaaattgtta attcaatttt acgaagaaca agcaacggat
      241 cgtcttcaag aatatgttga aaagttgatc aaaaatgaag aaatttctga aatgattagg
      301 aaacggctgt tattggaagc tgaaaaggcg gtgaaagaat gatcattgga ggaattttct
      361 ctggcatagc cgactggtgg tcaggtttat tcggagaagg ggatgcagat cccaaaaccg
      421 taacgaaagt cttacaagat tatggtaatt atctgcagta tcaagatgta ttcagttgga
      481 tttgtaatac ttttttatgg ggattgatca aagggttata taatctcaat actttattag
      541 aaaaaacaat ttatcaaagc tttgatttaa aagacttatt gaacgccgct ggtgttaatg
      601 aactatttca aagtttgatt agtaaggtat tggctctttt ttgtgtcgtc acattaattt
      661 atttaggctt gaaatttttt atgagtaaac acccaccgaa agtcaaaaat atcttggtta
      721 atttatttat ggccatgatc ttgatttttg gtggtagttc tttaatcgat gaagggttaa
      781 atatcagtaa aaacttttat ggagacgtca ctgcagcaaa taaagcagat aaaacgggtt
      841 cacctgcttt tcaaattatt caaaataatg tgattgatgt cagtacatta ctagcgactg
      901 acccgaataa agtagaatcc ttaccaacag ataaaagaaa tgctttaaca gcgaaaaatt
      961 tcaaaacagc gaatatcaat gggattatta cacctgatca agctaagaaa atgtctgaat
     1021 caagtgatat tagtacagta caaaaagatc ggttaaaggc attacagtat cgactagagt
     1081 tagacgataa tgggaaagaa atccctgttc aaattagtga tgaaggttta gcaaaatatg
     1141 tgtatacgtc aggctatcgt cgatacatta gtagtccagg gattattttg gctggggaat
     1201 cttctttgtc ggtagcctat ttatttattt tgtttacaat cgttacttgc attattgagc
     1261 tagtatttaa aaaattctat ttagtaattg ccgcgtcaac ggactttgag acaggacaac
     1321 ggatgaaaag tgcgatagaa gatatttctc aatccttctt gttattggcg tttacgattt
     1381 tagaattacg aatctataca ttaatgcttt caggtatcgg tgatttgcat gcggtaggaa
     1441 agatcaatgg attcttgtat gttgttgctt taattgtgct aactattgcg ctattcaaag
     1501 gctctcaatc ggttacgaag attttcggtg ttgatacgag tctgaaaaac ggcagcaatt
     1561 ctcttcttag catgtttgcc ttaggaaata ttgcgaaaaa tgtgggtagc ggagctaaaa
     1621 accttgcaag tgctggtaag agtggtttaa gccatttaaa tgatattcgt aagaatggat
     1681 ttggccatcg aaaagatatt acccatgata acggggaaga acaatcagac gataaagcta
     1741 caactgtacc taatagtaag aagacagttt ccgaaatgtt cgggaaagcg aaaaatggtg
     1801 tttctaaagc cgcagaaggt cttggttacg tcaatgaacg tgggttaaaa ggaacagttg
     1861 aagatcttac agaaaaagcc aaagacacct tagaagagaa attagacggc acgagagcta
     1921 aagaatttgc tgaagctgtg aagaatccat ccgaagcact agaaaaacaa aaagaaaaag
     1981 cttcagcagt caaagaagga ctaaaagatg cgtatggtga aggacaagtc aatgcagcag
     2041 tgaaggcaaa tacaacaagt gaaaaagaaa atcaagaagg tgcagcgatt ccttcagcca
     2101 aagatcacca agatgagtta cattctaacg aaaattcgat cggagaaaaa gattcagaag
     2161 aaggacaaca tggtcagtta ccaaaaaccg atccagaaca atatcgttat aaatctaaca
     2221 ttaattctga aaacgaaaaa cgcatttcag atttaccaaa acgaaatcaa gaagtacaaa
     2281 cggatgagga ttccatcgat ctgccaaatg ataatggcat cgaagttgga actgaaaaat
     2341 caacgaaaat tggtttgcct aagacacaaa atttaccaat gaaagggtac tgttcaaatg
     2401 aaatcaagtc gtttgcctga aaatggaaat ctgacaactg tttcttacaa tttagctgaa
     2461 agtggtgaaa cagagaatgc gtccgttcaa aatattgtga caaaagaagc tgcacctaag
     2521 ccaatcaata aagttcataa tggatacaga ccagcaaacg tagcatttac tgcaccgaca
     2581 aaaataaatg tgacaagaga aactaaaaac aggaacaatg aattgaaaga acaactattg
     2641 aaggatatca aaccataaaa ggcctattta agggcctttt tctttataaa acaagtttaa
     2701 aatggaaagg agaaaattgt ggaggatatt ttaatagcta cgtttataac tgcactggga
     2761 gcatttttaa cgatatttgt ggtgactatt aaaggattta aagatacgga tagtttacaa
     2821 aaagatcatc agtctatttt agatggtcaa aaaacacata ccgaaagcat tattgtacac
     2881 ctttcagaag gattcactta tttgaaagat gctatgccca cattcacagt aaaattaacg
     2941 gaaatatttc attctgttga gcaaatatcg tcagaattga aagagcaaag acaacaaaaa
     3001 gatcgtcaac ttggctcact taattttagg caagaaacga tacaagaatc attggagaat
     3061 gtaaatcgat taaacgatga aatgcaacga gttcatttgg aaaatgaaga attacgcgca
     3121 caaaattatg atttaattca aaagaaaaga aatctggaac aaaaaattgt tgaattaaaa
     3181 gaagaaaatc gtcaattgaa ttatcaatta cgacgaggaa aaaatcgttc tcgtgattta
     3241 ggaaatgaat tagagttgta atatttagaa aaacgctgtg agaaatcaca gtgtttttta
     3301 tttctaaaaa tacaaaatat aaggggtatg gttatattat ggataaatac attcatatga
     3361 aaaatcctcc aagatttagt ggggaatgga agtttttcgc ctttttcttt ttagatctat
     3421 taacgcttgg tggctgcttt ttttgggcga aaatagtcaa taatcttgtc cacttaccac
     3481 tcttttttgc actcttgaat tatggattat ggcttgcgat agcgttagtg attgtttggc
     3541 ga
//
LOCUS       DAZABD010000135         3410 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000135 DAZABD010000000
VERSION     DAZABD010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3410
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.141"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(627..1241)
                     /locus_tag="ACHALG_002845"
     CDS             complement(627..1241)
                     /locus_tag="ACHALG_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFN2588144.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1847..2224)
                     /locus_tag="ACHALG_002846"
     CDS             complement(1847..2224)
                     /locus_tag="ACHALG_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2588145.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2524..3405)
                     /gene="galU"
                     /locus_tag="ACHALG_002847"
     CDS             complement(2524..3405)
                     /gene="galU"
                     /locus_tag="ACHALG_002847"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFN2588146.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT         1037 a          686 c          509 g         1178 t
ORIGIN      
        1 attatttttt tctctttcca tttatattaa gtattttatt tctctgtttt tccttaaaat
       61 tcttttttta tttttagatt agagaaaatc tcgtcctgtt tttttcattt tacaatcatt
      121 gaaaaattga atttcattgc aaaaaaaata ataaacactt ataaatatcc attatctatc
      181 cttcaatttt tgtgtatttt atttctatta gaaaaaggta tcctgttgga caattttgtt
      241 atatcaggta aacttcattg caataatgat aacaaatgta ctataattac tttgtaaaga
      301 gttttgggga agactctttc atcaacgtgt attaagagac aagaaagaat ccatatggat
      361 tctttcttgt ctctttttcc actacttttt atttttctga cactacatat tcttataact
      421 catttctcat aactgtgaaa caacttataa aaataacaaa ctttaaacaa aacaagctta
      481 aaatcaaaga tgatccatct taaaataact aaaacaaaaa aagtagtcgt tgttaatcct
      541 cattaataat cgttttcttt ttttcaaacg ctccattttt atgtggaaca ttattttttt
      601 aaggaacttt gtcaacatgt ggataactat tttccacttg tgtcctggac tagctgtttt
      661 gctgtaaaat acaaaacttg ctgcagcaaa aagatcgttg caagagaggc tgttccgcag
      721 atcgctggat ctagatttgg caaagtcaag actgcatcat gtaaattggc gtggttgtcc
      781 aaaaccaaat cagcaaattc aaataatcgc aaaccactgc tatgttgcga tttgatactt
      841 cgtgacaaat caaatccagt aacaatgatt aatggatgac cattttcttt gatccactga
      901 gctagttcga tgattgccgg gtttcttcct tcattcgaaa gaagaagaaa aatgtcttct
      961 gatttgacag aaagctgatc aataattgcc tgtcctgcac cttctagcgt ttcatagaca
     1021 cctccggctg gattttcaat caatatactt ggtacaagat ccggggtttg atcccaaaat
     1081 tcaaatgcta caccacttag tgtgcggcta gcatatatct gcaatctacc accttgatta
     1141 atttgtttcg cgatccaagt gccagcttga agcatcagcg gctcttctat ccttaatttc
     1201 tcttccaatt ggtgataata gtcaaaataa cataaactca tttatctctc ccgcctagct
     1261 atacttgaca tgaatagaaa cagctgccta atctaaggaa gtacataaaa aaacagtagt
     1321 tagacagtaa cacatgtggt ctttgctgtt tcaaacacaa aaaaggcatt gaacgaatac
     1381 gcccaatacc ttgacatagc aaataaaaca acgtgcagac catggaaaat atctacatag
     1441 gggtagaatt ttcaccctag attaggtaca ataaattagg tcgatgcact gcattgacta
     1501 tggcaaaatg atcggtcagg gtgtatgtgt gtgactggtc attccctgat tcttagcgcc
     1561 aactaagggt cagggagcct ttttttattc agctgtttct atctgaattt attgtaagta
     1621 atctcttatc caagtgcaag caagaaaata caaaaaaaca ttggtatgat ttatatatat
     1681 ataaataaca taaaaaaagg aatggcttat cccgatacaa caagcattcc tttaaaaaag
     1741 attcttttat ataagtgaaa aaagcttttt tccaccaatt aaataaatct ctcatctcaa
     1801 tagtttatcc attattttat ctttttttgc gtatacctac taaatattac cgtcttcttc
     1861 tcgttcttct actttgttcg agagaatctg tactagaaaa agtagttgta tgtctacctt
     1921 ttctgcctat ccgagacttt cttttcatat atgggatata aatgattaga tacagtacag
     1981 caaaaaataa agcaaatacg caagcttccc aactacctaa cgccaattca accatcaagg
     2041 tagcaacccc aacggatgct gcataccaga taaaaatcag aaacgtttct ttttttctga
     2101 ccatgcgaaa catgataaat agactaataa gcaatagtgg caacacaaat atccaattgc
     2161 ttccttccat cagccacttg agtatatcaa gcagcgactg tgcaacagcg actaattttc
     2221 ccatagtgaa cacacacctt tttttataat taaaaaattc gcctttttat tctactatga
     2281 atcttctggc ttggctttca tttctcttat gtaatcattt tcttttatac aacttaaaaa
     2341 aaaattaaac cattagcata atattttctg aatctattca cgaaagtctt tttctatctt
     2401 tcgttttcca cgactcaact gatctagagt caaaaaacta aaacgacagt tgcgcaaatg
     2461 acgcacaagc tgccgcttta ataacaaata gcctttcatt ctcagcaggc cacttatttc
     2521 cagttaactt tctgttctta gctttttact caactggata atatatgcac ttaatgaagt
     2581 cccaatttcc gaatgttgaa gtccatattc aatatttgtc ttcatatatc ctaacttatc
     2641 gcctacgtcg tatctttttc ctttaaactc tctcgcatag accgcctctg tctggttaag
     2701 agtctgaagt gcatccgtta gctgtatctc gtttcctgct cctggttctt gagtttctaa
     2761 tatctcaaat attttaggag tcagtagata acgtccgata atggctaaat tacttggtgc
     2821 ttttcccatt tcaggttttt ctacaaaccc ttttactctg caacgaccct tttcgctatc
     2881 tgttttagga tcaataattc cgtatttaga tgtttcattt tctggtacac gcatgacggc
     2941 aacagcggaa gcttgtgttt cttcataatc catcatcaat tgttgagtca atgggatatc
     3001 atctttcatc aaatcatccc ccagcatgac gacaaacggt tcatttccta taaatgcttt
     3061 tgcttgaagc acggcatccc ccagtcctct tggatgagat tgacggatga agtgaagatt
     3121 gacttgagtc gtttcttcaa ctaaagcgag taattctgtt ttttcctttt cccgcaaatt
     3181 gttttccaat tcgatatttg catcaaagtg atcttcaatg ggtctttttt cttttcccgt
     3241 tacgatcaag atgtcttcga ttcccgaatc caatgcttct tccacaataa actggatcgt
     3301 tggtttatcc acgattggaa gcatttcttt tgccatggct tttgttgccg gcaaaaaacg
     3361 tgttcctagc ccagcggcag gaatgactgc ttttttgacc ttcacttgct
//
LOCUS       DAZABD010000136         3390 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000136 DAZABD010000000
VERSION     DAZABD010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.142"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            647..964
                     /locus_tag="ACHALG_002848"
     CDS             647..964
                     /locus_tag="ACHALG_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFN2588147.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNSSIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(1055..1420)
                     /locus_tag="ACHALG_002849"
     CDS             complement(1055..1420)
                     /locus_tag="ACHALG_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2588148.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            1628..2110
                     /locus_tag="ACHALG_002850"
     CDS             1628..2110
                     /locus_tag="ACHALG_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2588149.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            2307..3197
                     /locus_tag="ACHALG_002851"
     CDS             2307..3197
                     /locus_tag="ACHALG_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HFN2588150.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
BASE COUNT         1172 a          519 c          619 g         1080 t
ORIGIN      
        1 ttattgcttt tgcaaaattt atttacctgt tttctggatc aacgatagtg tacattatgc
       61 tatccttgtt tggattttag gacgtttaaa gaggcacttt tttacataac ttaatccata
      121 gaccttttaa gcatgctgtg agataataat aaagaagagt ttaaagcgca ccccaaacca
      181 cttccccata agtgtattac gctttaaact cttttatatt tgaattcact aagaagcata
      241 ccatatattt gaatttacta agaagcatac catatttttg aaaaaaagtg agaaaaaagg
      301 cttataatta ggatttagag taattaatta gatgtttatt ttataggact gatactatag
      361 attacaaata gtatcgtatt tcgcaatctt aaaattatct tcttactgtg aataagataa
      421 taaaatttgt atttgtcctt tttaggtaac ttattttaat ctatatacct ttctggatca
      481 acgaaagtat actttatata gtcataacgc cgatggtaac tatgcactgt aacgataatg
      541 ctgctaaaat atctttttac gaaaaacatc acaaggaaaa acttaactat ggtattatct
      601 aaacataatt ttttcggtat ttaatattta attaggaggt aatattttgc ttttttatag
      661 taattttatc ttaatagtag cgatattatt acttctgaat atatggattt ttgataggag
      721 tagaaattca agtataggtt ttagaactaa acgaagtttg tcttctaaaa aaaattgggt
      781 atactcacaa actatttttt atggaggaat tgttttaatt agtttacttt cttcaacact
      841 ttattctttg aatattatag atgtttccac ctcaaattca atatctatta ttggaatcat
      901 tattgctgca attattacac aactgttttt agtgtttgga gagaagaaaa gaagtaaaaa
      961 atagaaaaac tagtgatagt tttggtgata taagaaaaac atatatgtat aaaaagaaga
     1021 aagtagcttc tctttgtaca tatatggttt gtttttatgg atgccgagct tttttataag
     1081 ataaggctac cgcccataac gacccaagga tttgtggaac taagtaaata cctgcaagaa
     1141 gcaagatcag ctgggtcggt tttaaaggat gatagaagta acgccattca gtaaaattgg
     1201 cgcaaaataa ataggaagtg acagtagata taatattggc aatgaataaa gatataatac
     1261 gcctgttcct ttgaaaataa tatccagcaa aaagagataa aaatatcaat gtgatgaacc
     1321 cgcttacgga atgataacga aaatcaagca gtaaggcgaa atgcacataa ggaacaaaag
     1381 ataaaagcaa cggaaggttt ttccagcaag tttttatcat ctttcatcac cacctaacaa
     1441 gtgttattat tttgttagtt ttattgtaaa ttgaatcctg caataatcaa ttactttttc
     1501 attatttcta gattaaatga ataaactctc atgccattat ctctaaaaaa aaagtttttc
     1561 cccctaattt ttttagatag aaaattgtat agtatgatga gagaaaaagt agtttggggg
     1621 aacgttaatg agaagaaaag cagtaatcga tcggctttat ttattttttt ggttttttcc
     1681 tatcgttttg ctacctttta catgggtaaa tgctgttgta caaggtgcgg agatattagg
     1741 cctgatccaa ggaagcggat ttgtggtttc taggaatttt ccagctttga ttgttttcta
     1801 tttagtgata agtgtaggtc tcttattacc aaaatctctg atcaatcact acaattggca
     1861 gctgctttac ttttcttgct tattagtatt tacatctatg ttttctttgc tgcctcgctt
     1921 gattatcggc cctaatggtt tgagcgagag tggattgaag gatttagctt attcctccat
     1981 gtatttcttg acgatcaaac catggttcta tttagtaatt gcctcattta ttcttgcaac
     2041 atttttttac ttctggacag aaatcaggat aaaaagtgaa cgatctgtat ctataaaaaa
     2101 taactattga tggtggatca tcagtaaaaa aacatatgag ctgcgtaaaa tgcatgtttc
     2161 tcatatgctt tttttattat tatgtaaaga atgcgaaaaa cgtttacatg atttttacat
     2221 aacttaacta tatttttaca gcaaccatac gagggattta cagtcgactg ataaacttta
     2281 gttgtaaata agaaaggagc aataaaatga aaaaagtatt gtttactcta ggtatgatgg
     2341 gaattctttt agccggatgc ggctcaggat caggaaatac agatggctct gccacacaaa
     2401 ataattcaag tgactcaaat gaacaagtaa aaatcgttgc ggtaggttcg acagcattac
     2461 agccgttagt cgatgcagca caagaaagct ttgtacaaga aaatccaaat tatcaaatct
     2521 ctgtacaagg aggaggaagc ggaactggtt tgagccaagt cgaagctgga gcagttacta
     2581 tcggaaactc tgatgtattt gcagaagaaa gagacggagt tgatgcttca aaattagtgg
     2641 atcataaagt agcagtggtc gggatggcac caatcgtcaa taaagataca gatgtcaaag
     2701 atatcaccaa acaggaattg attgatatct ttacaggaaa aatcactaac tggaaggaag
     2761 ttggcgggaa agatcaaaaa atcaacgtcg tgaaccgtgc aaacggcagc ggtacccgcg
     2821 cgacattcga aaaatgggga ctggatggtg ctacccctgt gcagtcccaa gaacaagatt
     2881 catcaggaac agttcggcag ctggtaagtc aaacaccagg agcaatcagc tatttagctt
     2941 tctcatatct cgatgattcc acacaagcac taagtatcga cggtgtagaa ccaaaagaag
     3001 agaatgtagc agataacagc tggggaatct ggtcatatga acatatgtac acgaacggaa
     3061 aaccttcccc tgaagtccaa aaattcttag actacatgat gacagaagaa atccaagaag
     3121 gacctgtcaa agaattgggc tatttaccaa tcacaatgat ggaagtagaa cgtgatcacg
     3181 aaggaaatat caaataacta gcagataaac atgccacggt agtcgttact attaaggaaa
     3241 aatcaactat gtttccccac atatacattt ttcctttaat tgtgaaaaat agttcctcat
     3301 attttgccgt tttttgagct aataccgagg gtcagctgat tgaacaccgt ttattcgaaa
     3361 aaaagagcta cgacatagaa ctgtcgtaac
//
LOCUS       DAZABD010000137         3358 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000137 DAZABD010000000
VERSION     DAZABD010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3358
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.143"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2187..>3358
                     /locus_tag="ACHALG_002852"
     CDS             2187..>3358
                     /locus_tag="ACHALG_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFN2588151.1"
                     /translation="MTADPESLIITQKDGNVINLYYKKLSSDAEFYEPEVEKEEVNYG
                     GTIDLTDNVTNLEELPEGTKVNDVTPEGVIDTTQPGEYEGTIEVTYPDGTKDTVKVPV
                     EVVDNRNDAEKYTPEVEKEEVNYGGTIDLTDNVTNLEELPEGTKVNDVTPEGVIDTTQ
                     PGEYEGTIEVTYPDGTKDTVKIPVEVVDNRSDAEKYTPEVEKEEVNYGGTIDLTDNVT
                     NLEELPEGTKVNDVTPEGVIDTTQPGEYEGTIEVTYPDGTKDTVKIPVEVVDNRSDAE
                     KYTPEVEKEEVNYGGTIDLTDNVTNLEELPEGTKVNDVTPEGVIDTTQPGEYEGTIEV
                     TYPDGTKDTVKVPVEVVDNRNDAEKYTPEVEKEEVNYGGTIDLTDNVTNLEELPEGT"
BASE COUNT         1278 a          473 c          719 g          888 t
ORIGIN      
        1 tgtaataaat aaggaggaaa attatgttta atcaaaagaa agagaagcag ttcaaatatg
       61 caatcaaaaa gaaaaaaggt ggcgggggag cagcttcctt tgtcataggt gcagtatttc
      121 ttggaatagg gttaatttct tatcctacat tatcttttgc tagtgaaaca acagagataa
      181 atcaatcaag tgaaatccaa tctagtttga ctaattctgt tctaaaagaa agtaattcag
      241 aagaaaaaca aaatattgat atgtcggaag atagttctat aaataacgat ctagaactag
      301 attcgaaagc aaccgtttct ggatcagaaa attcatttga tgaaactact aacaatctag
      361 aaactacaaa tttatctgaa gatagtattg aagttcagtc agagagtatt attcaaaatg
      421 aagactcgac tgataatgat gctgttcaag aattagaaat gaatcctcat gaagataacg
      481 gtgatgatat ttcagttgag aatcagatga cagaagctac agaagaaaat ataaaaagta
      541 atactcaaga agtagataaa gatgcgacag ttttatctga tacaccaaga gatttagtta
      601 atttagagga cactagtaca gatatagaaa atgttactcc acaaaaaata ggtaaaaaag
      661 acagtacgtc tttatcagag gaaaatcttt cgtcaccagc atcaagtatt ttacgcagaa
      721 gaactaggag tttggatgtt caaactggag aaactttttt agaagattgg gcactaccag
      781 acaattctaa catttctgga agtttgatta ctcagactgg aattacttat gtgggtgtaa
      841 aaactgatga tcccactcaa ttacatttga aaatggctta cagagctgca gcaacaccag
      901 ctgcaattga ttggatttat attcgtgtgg atccagagct tacgaaatat attgaagaaa
      961 ttaacataaa aaatactgta aacatcggcg cgcaagttgg ttatcaacga tattttgaaa
     1021 aagtagatgc taatacattg agtgatgtaa atactttcgg aaccccgacc ggtgtaagtg
     1081 atgcttatgc acaagtagat caaacaactg gcggaatcta tcgtgtgtta ttagataaat
     1141 tttttacacg tattccaggt gtaaaaccta aggagcgagg tgcgttatct ggaaactggg
     1201 gtgtatttcc atcagttcct ctcgaggctg agagatatga agcaaatatc tatataaaat
     1261 tatcaaaaga tgtcgcagag ttacagcaag agcttggaag ggataagttt ggtgttcaac
     1321 ttcgtttaca aggtcaatca agttctggtg ttgcaactat tgctaaagaa agtttaaata
     1381 aaaatactat tattgatcta tcaaaacagg agtcaattga aaataattca aattggttaa
     1441 actcaccatc tgtgcataca attactacta cttatgaagg aaagcaaatg gggagcgatg
     1501 gtcagttcag taatactctc gaaggaactg ataaaagtct gattagggtt cagaatacaa
     1561 tcatgaataa tcttttatat ggaacggcac ttgatttagg tcgacctgtt tcatttaata
     1621 ttgagattga tccacaaata ttaaatttag tttcagatga tgattatatt acgatttggc
     1681 aagtatacag agaaaatagt atcaaccatc atgacggtgg atcaaatgca cctgtcatgt
     1741 tgaaaaagaa tatgtttgta gataataaaa ttaaaattga agtcaacaaa gataaagcgg
     1801 gtcagtatga aaacggagtt tactatgtga atgcggttga aagttttatg aatactgtga
     1861 gttcctctcc atttgttggt gcaaacttta ttgatattcc tgttatgcct aaacctttaa
     1921 ttgaagatga aggtcaattg gtatcagcag aaattaaaac atggtttacg gataaaaaaa
     1981 atgatcaaga gagggtagta agaaactcac ttgcttcaag ttatattaat ttattcccta
     2041 ctactattaa tttctatgat tattcaacca aagatacaga acaattattg aagttacgtg
     2101 aatcaaccta tttagtttct acgtttggtc aagaaattaa tggaatggat atgggggaaa
     2161 catacgaaat tagtaattat gagtttgtga ctgccgatcc tgaaagttta ataattacac
     2221 aaaaagatgg aaatgtcatt aatttatatt ataagaaact ttctagtgat gctgaatttt
     2281 atgaaccaga agtagaaaaa gaagaagtca actatggagg aacgattgat ctgacggata
     2341 atgtaactaa tctggaagag ttgccggaag gcacgaaagt gaacgatgtt acaccagaag
     2401 gtgtcattga tacaacccag ccaggtgagt atgaaggaac gatagaagtt acgtatccag
     2461 atggaacaaa agatacagtg aaagttccag tggaagtagt ggacaatcga aatgatgctg
     2521 aaaaatatac tccagaagta gaaaaagaag aagtcaacta cggaggaacg attgatctga
     2581 cagataatgt aactaatctg gaagagttgc cagaaggcac aaaagtgaac gatgttacac
     2641 cagaaggtgt cattgataca actcaaccag gtgagtatga aggaacgata gaagttacgt
     2701 acccagatgg aacaaaagac acagtaaaaa ttccagtgga ggtagtggac aatcgaagtg
     2761 atgctgaaaa atatactcca gaagtagaaa aagaagaagt caactatgga ggaacgattg
     2821 atctgacgga taatgtaact aatctggaag agttgccaga aggcacaaaa gtgaacgatg
     2881 ttacaccaga aggtgtcatt gatacgactc aaccaggtga gtatgaagga acgatagaag
     2941 ttacgtaccc agatggaaca aaagacacag taaaaattcc agtggaggta gtggacaatc
     3001 gaagtgatgc tgaaaaatat actccagaag tagaaaaaga agaagtcaac tatggaggaa
     3061 cgattgatct gacggataat gtaactaact tggaagagtt gccggaaggc acgaaagtga
     3121 acgatgttac accagaaggt gtcattgata caacccagcc aggtgagtat gaaggaacga
     3181 tagaagttac gtatccagat ggaacaaaag atacagtgaa agttccagtg gaggtagtgg
     3241 acaatcgaaa tgatgctgaa aaatatactc cagaagtaga aaaagaagaa gtcaactatg
     3301 gaggaacgat tgatctgaca gataatgtaa ctaatctgga agagttgccg gaaggcac
//
LOCUS       DAZABD010000138         3348 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000138 DAZABD010000000
VERSION     DAZABD010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3348
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.144"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..205
                     /locus_tag="ACHALG_002853"
     CDS             <1..205
                     /locus_tag="ACHALG_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2588152.1"
                     /translation="FLLLSFSIILSYLIVDLKLCNRTQGMALYAREGFKYFAPNGPGW
                     TMNIGIYYTYNPVAGTYYVVQNI"
     gene            330..2681
                     /locus_tag="ACHALG_002854"
     CDS             330..2681
                     /locus_tag="ACHALG_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HFN2588153.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            2761..3156
                     /locus_tag="ACHALG_002855"
     CDS             2761..3156
                     /locus_tag="ACHALG_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2588154.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
BASE COUNT         1167 a          422 c          663 g         1096 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc caaggaatgg cattgtatgc tcgtgaagga ttcaaatact ttgcaccaaa
      121 cgggcctggt tggacaatga atataggtat ttattacact tataatcctg ttgcaggtac
      181 gtattatgtt gttcaaaata tttaataaaa cagcacaaag agatttcttt gtgctgtttt
      241 attattttta gttgttgaca aacactaaaa tataaaatat aatcaacaca taaacgaacg
      301 tatgttcggc aagaaagcag gggattttta tggatgagat ggttttaagt actcagaaat
      361 ggctaaacaa gaaatacagt aatgttacag ggtttgataa ggttccagaa aatgggcgaa
      421 cagggtggcc tacaatctat ggacttattg aaggactaca agtagaactt ggtattacta
      481 atctagtagc taattttgga cctaccactg aaaaaatgta cgataatcag gttactccta
      541 aatggggcaa aaatctacct aaaaatattg tatttcttat tcaaggtgcc ttttggtgta
      601 aggggattaa tccaggtggc ttcgatgggg tatatactcc atatttagat actgctgtaa
      661 aagagttgca gactgatgca gggtttaatg gaagcgctgt aactggagtt ttggatgcga
      721 aatgggccaa agcgttattt gatatgagtg cttttgttct tgttcccggc ggagatagta
      781 aaattagaaa aatgcagcaa tggttaaatg taaattattt agaatacacg ggaataatgc
      841 cttgtgatgg tatttatcaa agaaattcaa atcaagcatt aatttttgca ttacaagctg
      901 aactaggata ttctcccagt gaagcaacag ggtcttatgg taatgggaca actagtaaaa
      961 cttatcctgt gtctgaaggt aactcgggaa actatgtaag aataattcaa tatggattat
     1021 atgttaatgg ttattttgaa gacggtacat tcgatggtat tttcactaaa tatatgggtt
     1081 tagaagtact ggcattccga aaatttatgg tattgcctga atatactgaa atagcagatg
     1141 ttgttgtaat aaaaggacta ttgtctagcg ctggagatat tagaagatct gcagatggcg
     1201 cagatacttc tacgcaatta actagatctc aaatccaaac attggttgat aatgatataa
     1261 aaatagttgg tagatatctt actggtactg ttggaattgg aaaagatgaa cgaaataagt
     1321 atctaactac agaggaattg aataatattt tttctaaaaa tttatctgtg tttcctatct
     1381 atcaagatgg aggtgcagca ttagcttatt ttacttatga tcgtggctta tcagatggta
     1441 agaaggctat agatgcagca aaaaatctaa atataccatt aacaacagtt atttattttg
     1501 ctgttgatct agacatgtta ggggaagaaa ttcttgcata tgctggagaa tattttaagg
     1561 gggtatctgc tgtaatgtca tatagcgggt atcaaacggg tgtttacgga acaagaaatg
     1621 tttgttctca aataatcaat aacgggtatg cttcgtttgc atttgtttca gatatgtcga
     1681 ctggttatag tgggaattta ggttttccta tgcctagaca atggtctttt gatcaatttg
     1741 ttgaatttac cataggaagt ggcaacggag ctgttggtat cgatttaatc gctacatcgg
     1801 gtagagatag cggatttaat gagttatcaa atgatactaa taatgataat tacattgcca
     1861 aatacaatca gaaggttata aatcagatgg tacgtgcgta tcaatatcta tcacaagctg
     1921 gtcttgataa tccttggaac cctcatttga cattttatag atatgtaaat tattctggtt
     1981 tgtcatggga catcatttct tctcctgtaa cagagcatga tagaaaaatt tatgatgaat
     2041 ataggaataa attaactaat agcgagggcc tgtataatta ttttatagat cctaatactg
     2101 gttcaataat aggtttgcct cacttaattg tgacgttaca atcacagatg tttctagccg
     2161 acacaatcaa tgatagtgtt tctgactttg caggttggtt aggagattta atgacttgct
     2221 ggggtgaagt taagaaatta ggaatacaaa tggagcaagg tgtatttgca ttagtaggta
     2281 cggctgcagg tggaacattt agtctagaag atttatacca agatgttgac gcggtaaatg
     2341 tttatacctt agcaaaaaga aattctgatt caacagcttt atcggcttta aaaacgtact
     2401 attgtgataa gggtgctatt gatagattta atcaatttgt taagaacaga tttggttctt
     2461 cagataaaat cgcttcagta acaaatagtt tattagaaaa tgacgaaaca gttgatatct
     2521 taggtgtagg gataatttca tttttcattg ataaaatagt taataattca cttgggacag
     2581 attttactta tcttgatttt attggagatg aggggaaagt tgcaaatgga ttttctgaaa
     2641 aaattattca atttcaagaa aatagaagta aacttaatta agaatcgttt actatttaaa
     2701 cagtgtcctt ttccttttta aatatatacg ataaaataag tatatgaatt gtaaaaggac
     2761 atgatgatta tggaagaaat agaatggaat aatctgactc aaaataataa aaataattct
     2821 ggatgtggaa ttatctttat gtttgttata gttttgggaa tagtagtact tttaataact
     2881 gtcaagatag tagattactt tgcgaatact cctgaagaac aaatacagag aaaacttcct
     2941 gagtttgaaa gagtaccacc ttatgatcca agagataatt catttcaagt aaaaaaatta
     3001 aaagagtctc caaatgaaat gtggtttgaa gttacagttg aatcaagcga cttcaaagaa
     3061 aaaaagaaag aatacaatta tattgtaaag gcaaaagtat caaaaagatt tatttttaac
     3121 cctaaattag aaatatatag tgttgtaata gaataatagt gttaaaaaaa ttgtaaaagt
     3181 taaagaagtc aaattctgat gtgaaaaata aaatatacca tggaagacat aaaagaaaaa
     3241 ctggataact agtttattta atggcagacc gttaaggcct gccattttta tgtattttag
     3301 cggaataaat ataatcatgc gggagtgtaa acttactgtt tcaaaaag
//
LOCUS       DAZABD010000139         3321 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000139 DAZABD010000000
VERSION     DAZABD010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.145"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            74..403
                     /locus_tag="ACHALG_002856"
     CDS             74..403
                     /locus_tag="ACHALG_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFN2588155.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            393..680
                     /locus_tag="ACHALG_002857"
     CDS             393..680
                     /locus_tag="ACHALG_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFN2588156.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
     gene            964..1821
                     /locus_tag="ACHALG_002858"
     CDS             964..1821
                     /locus_tag="ACHALG_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFN2588157.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            1822..2370
                     /locus_tag="ACHALG_002859"
     CDS             1822..2370
                     /locus_tag="ACHALG_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2588158.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            2630..2925
                     /locus_tag="ACHALG_002860"
                     /pseudo
     CDS             2630..2925
                     /locus_tag="ACHALG_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3000..>3321
                     /locus_tag="ACHALG_002861"
     CDS             3000..>3321
                     /locus_tag="ACHALG_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element ISS1N family transposase"
                     /protein_id="HFN2588159.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWVQEYSKVLYYLWKKKNRQSFYSWKMDETYIKIKGRWHYLYRAIDADGLTLDI
                     WLRKK"
BASE COUNT         1246 a          432 c          669 g          974 t
ORIGIN      
        1 cttgtaaata cattatctcg tggtataatg tatttactaa gtaattacat actgttagaa
       61 atgagggatt taaatgatta caaaaacaag aaaacaaggc aattcaatca tgttgacagt
      121 acctaaagat tttaatgttc ctaatggagt cgaagttgaa gcaaagttgg tagagaatgg
      181 tattctttat gaatttgttg aaccacaaaa agaattttat gattttagcg aagatatttt
      241 gtctgatata attgcagaag gctacgataa agatgagatt cttgtagaat ttaagaatag
      301 aaaaaacaaa atgcattctt cctttagaga tattgctgaa gatacactta ctaattctaa
      361 agtaatgact aaagaggagt tggcaacaga aattggctta tgaaatcgtt ccttcaaaac
      421 atgtcattaa gtatttaaaa aagctaaaag agaagccatt gaaagaaaaa tttttgaatt
      481 taatttatga tgaaattgcg attgatccat ataagggtga acaaaaaact ggggatttat
      541 caggtatttg gtctagtggg ttcaaatatg cgggcacaac atatagagta gcttacgaga
      601 ttatagaaaa caaggttatt ccagtattgt tatgtggaac gcatgaaaac ttttatgaac
      661 agttgaaaaa attaatctag tgatttgttg tcgttcttaa gaaggacggc ttctttttat
      721 cgaaaatctg attcgtctac atgactaaag tcgcgagtat tctctgcttc tcgataaatt
      781 aaataacgtc attactttga ggaaagctat aaatcaatat atttcagctt ttccttattt
      841 atgtactacc ttttacttat taattataag gtagtacata tatatctttt tttggtagta
      901 actaaaaata aatatgttat aatagtatca aatgaatatt gctaaaagaa aagaggtagg
      961 aacgtggtag cgaagaaata tgtaatcggt aattttaaag gtggcgtagg taaaagtacg
     1021 tgcgcgcaaa tgttagggtt tgaatcagca gttaatgaag gaaaaagaac cttgataatt
     1081 gacttggata tgcaaggaaa tacttctgat gtgatgaact taacgcatat gaacttcact
     1141 aacgaagaag gaggcggaga aggtgaaccg attgtttacg aaaatactgt gactgacgtg
     1201 ttgataaaca atcttccacc taaagaggct gtctataaag taatcaataa cctctatata
     1261 cttccagcag acatgtcctt tgaaatgtat gatgattgga tcaaagacca atataatgat
     1321 tcattatccc aattcaaata tatgaaagaa aagctagaac cattatttga tgagtttgac
     1381 gtcatctatt tagatgtccc accttctatt tctgtatata gtaaatccgc gatgtacatt
     1441 gcggactggg caatcgtcgt cttacagacc caagtaaaat cgatgagaaa tgcgatgcaa
     1501 tatcttgagt atatggactt ttttgtgaaa gaatttgata caaatttata tgttgctggt
     1561 gttattcctt tcatgttgga aagcggtgac gcagtagatc aggaaatgta taaacaagcc
     1621 caagagattt atggggaaca tttgattaaa aatgttgtac tcaaaaatgc gcgcttaaaa
     1681 cgatacgacg gatcaggtat tacgatggag aagacaaaga aaggtctctt aaaacaatgg
     1741 gatcgcaaaa gccacgaatt atttatcagt attttgaaag aattggaaga acacgagaat
     1801 tggtatgcgg aggtggagta attggctgac atcaaaggac ttgatatttt tgggaacaac
     1861 aaaggactcg ccaaaataaa aaaagaagca gaagcaaaga aaaagcagca agcaattaca
     1921 ccatctgaaa ataagcaatt agaagtgcac caagttcaag aaataaaaac ttcctccaat
     1981 gttgaaagac agagtagagg aagaacaaaa aaagttggcg caccaatcag aaagtttgat
     2041 aaggtttatt ccaccaagca tcctttaaaa ctgtcgactt tgttaaatgc gacttccaga
     2101 gttttagtag aaaagtatga gactaatatg acaagagatg aattgcttag aaaagcttta
     2161 gatgagtata ttaaacagaa tcttacgcaa gaagataagc aagatttgtt taatgacgtg
     2221 gtgaaagaac tagacttgtt cagagaaaaa tatccaattg ttccagaatt agatgatcgt
     2281 ggagaaataa taagaactac cagtcagata aaacaagata ccgaagactc actgcgcaaa
     2341 gggtggggaa tgaataataa agttagataa taatcaaaga agaactaagt aactaccttt
     2401 aaacggtagt tacttagttc ttctttgatt tgcatgaaat agaggggatt tatttttctg
     2461 gttacgtgat taaaaaatcc aaaaaatgag cataggaacg gttatgattt tagggtgtgt
     2521 tttaatgaga tattgtgatt tcattaacaa gtgtatgata atctgttgtt gagttagtaa
     2581 ataagtaata gagctaactc taaaataaaa ctattaggag gagatgtagg tgaaaaagtt
     2641 taaaaagaaa atatatttag gattggttgt tggggtgagt atgtttgggt tcatggctca
     2701 tactaaacaa gtaactgcag atactaacca aaaagaaagg attatgagcc aattagatga
     2761 ctctcaaata agtatgtacc caatgagcta attgtcagtt taaaggctac acaaagaatt
     2821 tcaacagaga aaaataaacc gcaaaattta ttgggtgtaa aagttatggt ggaaaaagat
     2881 ttgactctta ggacaaatga aagcgattgt aataagcgag atcaagggtt ctgttgcaaa
     2941 gttttccaaa aaatctattt tagtgtaaaa ttgagaaaaa agacagagag gacagagtaa
     3001 tgaatcattt taaaggcaaa caattcaaaa aagacgtcat tattgtcgct gttggttact
     3061 acctgcgtta caatctaagc tatcgtgaag ttcaggaatt gttatatgat cgtggaataa
     3121 atgtttgtca tactacgatt tatcgttggg tacaagagta cagcaaagtc ctctattatc
     3181 tttggaagaa gaaaaataga caatccttct attcatggaa aatggacgaa acctatatca
     3241 aaattaaggg acgttggcat tatctttatc gtgcaattga tgcggacggc ttaaccttag
     3301 atatctggtt acgaaagaaa c
//
LOCUS       DAZABD010000140         3291 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000140 DAZABD010000000
VERSION     DAZABD010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.146"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(553..2166)
                     /locus_tag="ACHALG_002862"
     CDS             complement(553..2166)
                     /locus_tag="ACHALG_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFN2588160.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
     gene            complement(2307..3215)
                     /locus_tag="ACHALG_002863"
     CDS             complement(2307..3215)
                     /locus_tag="ACHALG_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN2588161.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
BASE COUNT         1069 a          563 c          576 g         1083 t
ORIGIN      
        1 gttaaataaa taggaacaaa aaaattaaat tatttaatac acacattagg gatcattcat
       61 tgaaggatta gaagacaacg aggatggcga aaaatatgat tacaatagaa aagtcgttct
      121 aaaccaatca gttcctagta tcacacaacg caagccagtc gatttggtga gattagagga
      181 gtgaaagcaa actttattat ataagaaaaa atattcgaaa gggatgctag tttaggtact
      241 agtgctgata gattaatatg atgcctcgta atattaggcg tagttactct agagacatca
      301 ctatggacta tgatttgtct cagagtcaca acatgttttt ttatgaaaag aaacagctaa
      361 aataattaaa ttgcgcaatg tttaaacttt cttaaactat aacctaataa atgtgaagaa
      421 ataatgcaca atttcttcac atttatcttc tattctatat acataccaac caacaaccct
      481 atatactttt tcatttttac tcctcaagta aaaataactc ctagaccgcg gctacccacc
      541 gcggtcgttt ttttattctt caatcaaaat cgctttgata cccaaacctg ccaagattcg
      601 ggcagaagta taagtattca atcctcttct gaaagtaatg taaacttttt tatcttttgg
      661 gatttcattg attcgatctc gtaactcatt cattggaata ttttttgttg ccgagatact
      721 gccagaaggt gctttattag gttcacgtat atctaaaaag aacgcttctt tcaaatcgtt
      781 ttcgggtata tcacttgctt tgatcgtttc tacgatattc gtcatttgat tgattgcgac
      841 ataaccagct atattcaacg ggtcacgggt agtagaatac ggtggagagt aaggcaactc
      901 caaatcaggc aaatcaaaga ctgtcaagtt tccggtgatt gctacagaaa gttctcccat
      961 ccgtttatcg acaccttttt ctccaaatgc ttgtccacct aaaatacgac cgctatctgc
     1021 atcaaaaatc agctttagat ttaatcttgt agcttctgga tagaaatagg catggtcaaa
     1081 gggagtgata aaaacagttt tataatttgt tatgcctgat gcttttaatt catgttctgt
     1141 cattcccgca taacttgctg tatgatcaaa gatttttgcg acacctgcac caatatatcc
     1201 gcgatagcgc ataggcgtac catttaaaat atctgccagc atatgtcctt gacgattggc
     1261 cgcactggaa agcatacttt ggatcggctg accagtcact acactagtcg tttcgataat
     1321 atcgccaatc gcatagatgt ccggcagatt ggtttgtaac tggtcatcaa caatgatctg
     1381 acctgtatct gataattgta tgccggttgc tttcaccact tcattcttcg gagaaacacc
     1441 aacagcaaaa attagcatat cagcagaaag gacggagcca tctgataata ctatttctcg
     1501 acctttatca cggatctcag taactcttgt ttctaaatga acggctaatc cttctttcag
     1561 caatttgtca taaactaaat tagcaatttc tggatcataa gggaaagcga cttgtggcaa
     1621 ttgatcgatt aaagtcacgt ctaagccgcg atgcttgaaa ttctcagcta attccagacc
     1681 cataacacct gcaccaagaa tgacgacatg ttgtggattt ttttcttcaa ggaacgattt
     1741 gattcgatcc gcatccgtca cactacggag gacaaagccg ttttctgctt caaaaactcc
     1801 gggaatatcc ggataatctg gtctagcacc agaagagatg atcaacttat cgtaatctgt
     1861 ttttatttct tcatttgtgg ataggtcttt aactgttaat cgcttagtag aaggatcgat
     1921 tgctgttact tcgtgtttag taaagacatc gatgttgttt tttgttttca atatttctgg
     1981 tgtacgttcg atcagcgagt cacggtcagt gatcacacct cctaaataat aaggcaaagc
     2041 acaacttgca taagaaatat tttctccgcg ttcatagata atgatttcgt gcgcttcatt
     2101 caatcgacgg aaacgagtgg caaatgaagg accgccagct acaccgccaa tgacaacaac
     2161 tttcataaaa acatctcctt ttctacgttg atcgtactat gttatggtaa aaaacagcaa
     2221 ataattgtgc cggaaaatta ttgtttattt tgaatagaca gaaaatatag agaaataatt
     2281 tgtggataaa acctaaaaat ttttttctat tttaaggata gataatactt tgaaggggat
     2341 aaccctgtat attttttaaa agctgaagaa aaatgatgga tcgaatgaaa tcctaatata
     2401 tctgcaattt ctgtataggt taattcattt tctagcacga gacgttttgc tttttccatt
     2461 tttagttctt taaaatagct aataacactt tttccattca cttttctaaa atgttttttt
     2521 aagctggctg gactgataaa aaaaatttgg gctaattgtt caagcaaaaa attttctgaa
     2581 agatgttctt ctaaatatcg tatgattgga tcaactaatt catctgaata aagaatatgc
     2641 ttttttttag gaatgacttt ctcatttgaa ggtattgtca ataaagcaag ttcctgttga
     2701 taatgccttg gtaaggaaga caattgaatt ttttgcagac aataagtaac taataaggaa
     2761 agcaaatgaa tgctcatctg ttggcatagt atgaaaaaag aattgatttc ctgatcatag
     2821 gaaatagctg gcttactatg ttttagataa tcaattttga actgttcatt tttcagaaaa
     2881 gtaatgatag atgttatttc ttgatctata gaaaaagatt gtcccgtaat atgtgctaat
     2941 aattgctcat caaaataaaa ctggataatg ataaaagttt gtgtttcctt ttgagtagaa
     3001 aagatttgga cttcttgctg tttatcaatg aataaaaggg aaggattatc tagttctata
     3061 gatttatgtg caaattcagc atataaatta gcgctttcat ttattagtag tcgaaaatag
     3121 ggagcagcag ataaatgaag atgggtatct atggaatagg ttagtgtttt tttgacaaga
     3181 ttggaggatt ggttgtttgt taacaaaaga accactttcc tttctctttt tatataaaaa
     3241 gctttctatt tataattaat gactattata acaaaaatga aaagccaaaa c
//
LOCUS       DAZABD010000141         3283 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000141 DAZABD010000000
VERSION     DAZABD010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3283
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.147"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            3..587
                     /locus_tag="ACHALG_002864"
     CDS             3..587
                     /locus_tag="ACHALG_002864"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFN2588162.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            complement(664..1839)
                     /locus_tag="ACHALG_002865"
     CDS             complement(664..1839)
                     /locus_tag="ACHALG_002865"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFN2588163.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            1921..2301
                     /locus_tag="ACHALG_002866"
     CDS             1921..2301
                     /locus_tag="ACHALG_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvfD/Ygs/GSP13 family RNA-binding
                     post-transcriptional regulator"
                     /protein_id="HFN2588164.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            2375..2737
                     /locus_tag="ACHALG_002867"
     CDS             2375..2737
                     /locus_tag="ACHALG_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2588165.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
BASE COUNT         1115 a          538 c          622 g         1008 t
ORIGIN      
        1 atatggcatt cccacaacta gatttaaaaa gtgaaaaagg accaaaagct gttatcaaaa
       61 caaaccgtgg tgacattact gttcaattat tccctgaact agcaccgaag acggttcaaa
      121 atttcattga actttccaaa aaaggttatt atgatggcgt gatttttcac cgcgttattc
      181 ctgatttcat gatccaaggc ggcgatccaa ctggaacagg aatgggtggc gaaagtattt
      241 atggcgaaag ttttgaagat gaattcagtc gtgaactgtt caaccttcgc ggagctttat
      301 ctatggcaaa ttcaggtcca aacacaaacg gaagccaatt ctttattgta aataacacaa
      361 atgtgccagc taatatgctt ggacaactag aaggtgctgg attcccatca gaaatcattg
      421 aagcgtataa aggtggcgga actccttggc tagatttccg acatacagtc tttggacatg
      481 tactagaagg gatggacaca gtagatgaaa tcgcaaatgt acaacgtggt ccacaagacc
      541 gacctgtaca tgatgtagtg attgaaacaa ttgaaatttc agaatagttt ggtatggtac
      601 ggagtaatta tgaaaaagga cgaaacctag taaggtctcg tccttttatt ttgaataaat
      661 gatttaatcg attgtctcta aagcatactt gattcgatta agtccttcca gtaaagtttc
      721 ttctggacaa gcaatattca agcgcatatg cccatgaccg gaaggaccaa acgtgatgcc
      781 aggatttaat acgacttttc ctttccgaac aagagtgttc tccaattctt ggtcgtctga
      841 aatataggcg gtaaaatcta accacattaa gtaagttcct tcagggttca ttattctcac
      901 tttcggaaga tactgttcaa aaaactgatt aactgtttga acattttttt ctagataagg
      961 aatcagttgg cttaaccatt ctccacccgt ctcataagcc gcttgggttc caatcagtcc
     1021 aaaagtgttg atttcgtgct gttggttcat cactaaatgt ttttcaaaat cagctttcat
     1081 ctctggattt ttgataaata ccattgaatt tttgattgca gctagattga aagtcttggt
     1141 tgcggaagta aaagcaacta gtagatcatc taaattagga tcaatcgtta gcatggaagt
     1201 aaatgtatga tcgaataaag ttaaatcttg atggatctcg tcactaaaca gaatcacttg
     1261 atgttttaaa cacaattcgc ttaactgttt caattcttct ttgctccaaa cacgccctcc
     1321 aggattgtga ggattgcata aaatcattgc ttttacattg ttttcttcga tttttttctc
     1381 catgtcagac aagtccatga tgaaatgatt atttttttct agcaaagagc ttcgaacgat
     1441 gtgacgttga tttgtttcta taatagagga aaatggagga taaactgggt catgaatcaa
     1501 aatagaatcg cctggttttg taaaagtttg tactgctgca gctaaactgg ttaacacacc
     1561 acttgtaaaa acgatatttt ctttcgtcaa ctccacagca tggtgttggc gttcccaatg
     1621 aatgatagcc tgatatagtt tatcagacag agtggcataa ccaaagattc catgtttgat
     1681 gtattcacta aacgctttag ctaccccacc gggagacaag aagtccatat ctgctaccca
     1741 caaaggaagt aagtcatcca tttgatatgt ctcagcaatt gcgtcccatt ttacactttc
     1801 tgtatctttt cttgaaatct gtttatcaaa ttcgatcatc ttaagacctc cagtcatttt
     1861 cgtcgcttct ttttttgagc tatttacagt ataatgataa atgcgaagag agggaagtag
     1921 atgacttata aaattggaca aataattgaa ggaaaagtga cagggataca gccttatggc
     1981 gcattcgttt ctcttgataa cgagacgcaa ggacttattc atgtttcaga agtacaatcc
     2041 ggttatacga aaaatatcca ttcattgtta aaagtcggac agccagtaac agttcagatc
     2101 atcgacattg atgaatattc caaaaaaatc agtttatctt taagaacgtt agaaaaaaca
     2161 aatccagctg ttccatatcg cagaaaaagg tatttcacga ataaaaatcg taagatcgga
     2221 tttaccacta ttgcggagca gttacctatt tggatagaag aagcattatc tgaactagaa
     2281 cggaaagaaa ataagaaata aaaagtgtat tttttctgaa aatgtgctag aatttttatg
     2341 taagccatag aacaagttga tcgaggtgga aattttggca agcaaaagag ttgctggtac
     2401 aattatgttg catttggagg atggtactaa aaaatttttg gtccattcaa ttgatgacaa
     2461 gttggaattt gcgttagcgg aactttctga aggaaagact ggtttagcga atatacttaa
     2521 tttcttaaaa gaaatagttc atatcgatgt tagcaagatt agtttgatgg aattgacaaa
     2581 tacacatatt aaagaggaaa atgttccgtt gtttgtcttt gaaactgaac aaaagaatca
     2641 aagagaagaa ttaggcgaag gatatatttg ggaagaacca ggtcaaatgc gctctgttct
     2701 tggaacttac gaagtagagg gagtgccttt cttctaaaat acagattaag cgaacaaaga
     2761 agtttgaatt tttttcaaac ttcttttttt attgtttggt ccaaaaaata caatgaaatg
     2821 ttctgctaaa gatagttttt tagtgcattt tattttcaaa atatgttgaa aacggttgta
     2881 taacgaataa taataattga tcgaaaaatc atttcacaaa gtaatgctta atttatagtt
     2941 gaccaaggaa gaaatacatg ataaatttaa cagcgttgat tctgaagtgg atgttatatc
     3001 acatttctaa aaaatttaaa aaatagttat tgacatgtta ctaacgacat gataagataa
     3061 ctgagtcgtt aaaaaagaac cacttgcgaa gcttgattct atcgaaaaaa acttcaaaaa
     3121 agttcttgac aaataacaaa cgataagtta tgataaatga gttgctgaaa gatttcgaaa
     3181 aacgaaatcg aaaaaaactt ttaaaaaaga gttgacaatc gcttgtctct ctgatatgat
     3241 ataaaagttg ctacggcagc caaaaacgaa tcgggaaaca ctc
//
LOCUS       DAZABD010000142         3233 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000142 DAZABD010000000
VERSION     DAZABD010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.148"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..411
                     /locus_tag="ACHALG_002868"
     CDS             <1..411
                     /locus_tag="ACHALG_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2588166.1"
                     /translation="GLYYSPVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAP
                     QEYLSRAYNDAPKYCANIVDTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLT
                     ELKGYHVTTGEPVCQPRRPNSRIDKVRYMFNKIT"
     gene            518..571
                     /locus_tag="ACHALG_002869"
                     /pseudo
     CDS             518..571
                     /locus_tag="ACHALG_002869"
                     /inference="COORDINATES: protein motif:HMM:NF019662.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance efflux system leader
                     peptide"
     gene            605..1981
                     /gene="tet(L)"
                     /locus_tag="ACHALG_002870"
     CDS             605..1981
                     /gene="tet(L)"
                     /locus_tag="ACHALG_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001574277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HFN2588167.1"
                     /translation="MNTSYSQSNLRHNQILIWLCILSFFSVLNEMVLNVSLPDIANDF
                     NKPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSF
                     FSLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGG
                     MIAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFT
                     TSYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFV
                     SMVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLS
                     VSFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFT
                     SFLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNV
                     YKHSQRDF"
     gene            2545..>3233
                     /gene="mobV"
                     /locus_tag="ACHALG_002871"
     CDS             2545..>3233
                     /gene="mobV"
                     /locus_tag="ACHALG_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="HFN2588168.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYS
                     DKVKSDLEVPA"
BASE COUNT         1031 a          490 c          654 g         1058 t
ORIGIN      
        1 ggcttatact atagccctgt tagtacccca gcagattttc ggatgcttgc tcctattgta
       61 ttggaacaag tcttaaaaaa agctggaaca gaattgttag agccatatct tagttttaaa
      121 atttatgcgc cacaggaata tctttcacga gcatacaacg atgctcctaa atattgtgcg
      181 aacatcgtag acactcaatt gaaaaataat gaggtcattc ttagtggaga aatccctgct
      241 cggtgtattc aagaatatcg tagtgattta actttcttta caaatggacg tagtgtttgt
      301 ttaacagagt taaaagggta ccatgttact accggtgaac ctgtttgcca gccccgtcgt
      361 ccaaatagtc ggatagataa agtacgatat atgttcaata aaataactta gtgtatttta
      421 tgttgttata taaatatggt ttcttgttaa ataagatgaa atatttttta ataaagattt
      481 gaattaaagt gtaaaggagg aattaattat gaagtgaaat gaatgtaaca gggttcaatt
      541 aaaagaggga agcgtatcat taaccctata aactacgtct gccctcatta ttggagggtg
      601 aaatgtgaat acatcctatt cacaatcgaa tttacgacac aaccaaattt taatttggct
      661 ttgcatttta tcttttttta gcgtattaaa tgaaatggtt ttgaacgtct cattacctga
      721 tattgcaaat gattttaata aaccacctgc gagtacaaac tgggtgaaca cagcctttat
      781 gttaaccttt tccattggaa cagctgtata tggaaagcta tctgatcaat taggcatcaa
      841 aaggttactc ctatttggaa ttataataaa ttgtttcggg tcggtaattg ggtttgttgg
      901 ccattctttc ttttccttac ttattatggc tcgttttatt caaggggctg gtgcagctgc
      961 atttccagca ctcgtaatgg ttgtagttgc gcgctatatt ccaaaggaaa ataggggtaa
     1021 agcatttggt cttattggat cgatagtagc catgggagaa ggagtcggtc cagcgattgg
     1081 tggaatgata gcccattata ttcattggtc ctatcttcta ctcattccta tgataacaat
     1141 tatcactgtt ccgtttctta tgaaattatt aaagaaagaa gtaaggataa aaggtcattt
     1201 tgatatcaaa ggaattatac taatgtctgt aggcattgta ttttttatgt tgtttacaac
     1261 atcatatagc atttcttttc ttatcgttag cgtgctgtca ttcctgatat ttgtaaaaca
     1321 tatcaggaaa gtaacagatc cttttgttga tcccggatta gggaaaaata taccttttat
     1381 gattggagtt ctttgtgggg gaattatatt tggaacagta gcagggtttg tctctatggt
     1441 tccttatatg atgaaagatg ttcaccagct aagtactgcc gaaatcggaa gtgtaattat
     1501 tttccctgga acaatgagtg tcattatttt cggctacatt ggtgggatac ttgttgatag
     1561 aagaggtcct ttatacgtgt taaacatcgg agttacattt ctttctgtta gctttttaac
     1621 tgcttccttt cttttagaaa caacatcatg gttcatgaca attataatcg tatttgtttt
     1681 aggtgggctt tcgttcacca aaacagttat atcaacaatt gtttcaagta gcttgaaaca
     1741 gcaggaagct ggtgctggaa tgagtttgct taactttacc agctttttat cagagggaac
     1801 aggtattgca attgtaggtg gtttattatc cataccctta cttgatcaaa ggttgttacc
     1861 tatggaagtt gatcagtcaa cttatctgta tagtaatttg ttattacttt tttcaggaat
     1921 cattgtcatt agttggctgg ttaccttgaa tgtatataaa cattctcaaa gggatttcta
     1981 aatcgttaag ggatcaactt tgggagagag ttcaaaattg atcctttttt tataacagga
     2041 attcaaatct ttttgttcca ttaaagggcg cgattgctga ataaaagata cgagagacct
     2101 ctcttgtatc ttttttattt tgagtggttt tgtccgttac actagaaaac cgaaagacaa
     2161 taaaaatttt attcttgctg agtctggctt tcggtaagct agacaaaacg gacaaaataa
     2221 aaattggcaa gggtttaaag gtggagattt tttgagtgat cttctcaaaa aatactacct
     2281 gtcccttgct gatttttaaa cgagcacgag agcaaaaccc ccctttgctg aggtggcaga
     2341 gggcaggttt ttttgtttct tttttctcgt aaaaaaaaga aaggtcttaa aggttttatg
     2401 gttttggtcg gcactgccga cagcctcgca gagcacacac tttatgaata taaagtatag
     2461 tgtgttatac tttacttgga agtggttgcc ggaaagagcg aaaatgcctc acatttgtgc
     2521 cacctaaaaa ggagcgattt acatatgagt tatgcagttt gtagaatgca aaaagtgaaa
     2581 tcagctggac taaaaggcat gcaatttcat aatcaaagag agcgaaaaag tagaacgaat
     2641 gatgatattg accatgagcg aacacgtgaa aattatgatt tgaaaaatga taaaaatatt
     2701 gattacaacg aacgtgtcaa agaaattatt gaatcacaaa aaacaggtac aagaaaaacg
     2761 aggaaagatg ctgttcttgt aaatgagttg ctagtaacat ctgaccgaga tttttttgag
     2821 caactggatc caggagaaca aaaacgattt tttgaggaaa gttataaatt attttccgaa
     2881 cgatatggca agcaaaatat tgcttatgca acagttcata atgatgagca aacccctcac
     2941 atgcatttag gtgttgtgcc tatgcgtgat ggaaaactgc aaggaaaaaa tgtgtttaat
     3001 cgtcaagaac tgttatggct acaagataaa ttccccgagc atatgaaaaa acagggtttt
     3061 gagttgaagc gtggtgaacg tggctctgac cgtaaacata ttgagacagc taaatttaaa
     3121 aaacaaactt tggaaaaaga gattgatttt ctagaaaaaa atttagcagt taaaaaagat
     3181 gaatggactg cttatagcga taaagttaaa tcagatttag aagtaccagc gaa
//
LOCUS       DAZABD010000143         3160 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000143 DAZABD010000000
VERSION     DAZABD010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3160
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.150"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..358)
                     /locus_tag="ACHALG_002872"
     CDS             complement(<1..358)
                     /locus_tag="ACHALG_002872"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFN2588169.1"
                     /translation="MTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKEGISVSL
                     KHVQKLMKQLNLRSIVVKKYRPQRSNKPIILKENLLNQDFSTETICEKWAADITYIPT
                     KKNGWCYLSSIMDLHTK"
     gene            complement(469..723)
                     /locus_tag="ACHALG_002873"
     CDS             complement(469..723)
                     /locus_tag="ACHALG_002873"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFN2588170.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            complement(803..1156)
                     /locus_tag="ACHALG_002874"
                     /pseudo
     CDS             complement(803..1156)
                     /locus_tag="ACHALG_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1269..1526)
                     /locus_tag="ACHALG_002875"
     CDS             complement(1269..1526)
                     /locus_tag="ACHALG_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2588171.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(2123..2401)
                     /locus_tag="ACHALG_002876"
     CDS             complement(2123..2401)
                     /locus_tag="ACHALG_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2588172.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(2435..2686)
                     /locus_tag="ACHALG_002877"
     CDS             complement(2435..2686)
                     /locus_tag="ACHALG_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2588173.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT          980 a          615 c          433 g         1132 t
ORIGIN      
        1 ttttcgtgtg taaatccatg attgaagata agtaacacca accatttttc ttagtaggaa
       61 tgtacgtaat gtcagctgcc catttctcac agatcgtttc agtagaaaag tcctgattta
      121 agagattctc ttttaaaatg atcggtttat tagacctttg aggtctatat tttttaacta
      181 caatcgacct taaatttaat tgtttcatta gcttttggac atgtttaaga gatactgaaa
      241 tcccttcttt taataaaact tgatggatct ttgtggcacc atagcgttgt ttattcgtaa
      301 aaaaaattgt tgaaatcttc tttttcaact tattatttct gatttctgta gctgtcaacg
      361 gtttattctt ataaaaataa tagacacttc tagggatttc taataaccga cacaacaaag
      421 aaacagtgta atccttacac catttttcaa taaattgaac aagcgaattt aggttttccg
      481 agagaatatg gctgcggctt tttttaagat ttcaagttcc tcatttaaac gagcattttc
      541 ttttctcagt tcagctactt cttttccagt cagtcctgtg gactgatcag gtaaatataa
      601 attcttccat ttgtaaatcg ttgcttcaga taagccatat tctttcgcta accctcgaac
      661 agaacgtcca gtttgattca gttcaacaat catttgttta aaattttctt catatcgggt
      721 cataaaaatt catcctctcg tagataagtt taatttattc ttgtctctac gaaaagctgt
      781 ctaagatttg atactaacac caatcgtccc caaaaggtag atcttctcta ccactagcta
      841 atctacttct ccatcttcgt tctcttttca tagaacctgc gctgaactca cacccgttaa
      901 actcagctat gacagtatct tcaattatgt taatctgtcc atctagttcg tattcaatcc
      961 taaatggaaa ttctccatag gtgatttccg gttcgggtat tttaggtgag attaatgcaa
     1021 agaaaggaaa tattaagctt ggcaacatca aaaaaactcc tcctatgatc acaattcccg
     1081 atgtaatcca aaatataatt tgtacaattc tcattgatcc attgtattcc attttattcc
     1141 ccctattact attcatatcc gcctatctat atcgatcttc caatgcagag ataattgctt
     1201 ccataccttg attttttact gagttaccta tgcgagatat cattctccac taaggtgcat
     1261 catattgtct atctaacatg attaattctc caataggtgt atactcctcc aaaaacaaca
     1321 tttaatgcat ctccctcgac aacataagct accatgtttg ctgtatatgt gtttgagtct
     1381 aattcataag cagataaatt tactgtcgct ggattaaata taatcgcatt cgtatttata
     1441 gctactgaat ttgctgcttc agcaccacct tttgaatgtc cgatcattgt gatttcaata
     1501 gtaggattta cgtggacagc cctcaccgtt taccctgacc attatagaaa tcgcttactt
     1561 ttttgaacta cgcaacaaag gatacaatgc tcctaacttc actgtctcca tttttgtcca
     1621 ctaaggaata taaaaaatag gcattaattc atttaattcg gtcacaacac gttacctttt
     1681 aaatcattat actgtatctc atacattatt atcaataaac tattataaaa atgcaaaaaa
     1741 tgctttatca atctattttg taattggtca taatatcatt gaaatcatac cctgtatcga
     1801 tttttcctat gaatctcgtc tatttgtgat tgacgaaaaa gaggcaagac agacactgga
     1861 atggtaattt tacccactat tttgctccaa ctatttgtaa tcgtacccac ttttgaaata
     1921 ccgagatgat cattttaatt gatatgctga ttcccaagca aaaccccaag aaattaaatg
     1981 ctctatcgca tctagcttct tgggttttgc ttatataaat tccaacattt aaatttttta
     2041 tgctgttcgg tctcagaaat gccgtttttg cttatatcac aatatcaaga tgtcaaatta
     2101 ttcgtgttgc cgatgacttt gattaatttt tttcaaattg tttattaaga ctaatgctgt
     2161 ctttttgcag ataccaatgt tcgaactttt taacattatc atatgatcct gcaagtttta
     2221 acttcttacc attagatgtt atcaaagtta taattaacct tgcctttttc ccattaggat
     2281 cttttatttt atgaattttc tgcaggtaat ctctcctaat ggcagctatt tttacctcat
     2341 cagtgctgat aaaccattga tcatccacat acatactgcc tgttatgttg tgtttttcca
     2401 tttcttccga gaacgaaaag tttaaggctc gttcttactt ccaaagttga atgagtccat
     2461 ggattggact tagtgtgaga attgagaaca acaataagaa tccatatcct cccgcaaaca
     2521 tccctaaggc tatactgtct ctccatccat aagtaggatt agaagagaaa agagtcaccg
     2581 tttgtaataa tatgaaaagg aataatccag cacaaacacc aaagataaca actccaaaca
     2641 ttgaattttt atacaatgac catttattta taggcttttc tttcatttta cttcccccta
     2701 ctgttctgtt cacacggaat gaattcaaag ttcataagcc ctcctctttg aacatatgaa
     2761 tacgacttgc taactatact ttaaaaaagc ttgttttaag cggttatagg cgagttgtaa
     2821 tctctattca tctcattagc aaataacttg ccgaaaagtc tactgaaatt aaagcagata
     2881 gtggaagaat tatttagcca ggatagacac tatttgtgaa acacatgtct ttacttttga
     2941 aattctatga tgaaaagcct tggaatacag tagctcttaa ttggtaatac tttttccatt
     3001 ataaataacc tcttttcatt ttatatttat cccctctaaa aataatatct aatctaatat
     3061 tttaaactta tatttccctc gctgtgctaa tagagcatta ctaaataaat agaaactaga
     3121 aaaaagcatt acgcctgcca gaataaacca attatttgta
//
LOCUS       DAZABD010000144         3093 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000144 DAZABD010000000
VERSION     DAZABD010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3093
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.151"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..835)
                     /locus_tag="ACHALG_002878"
                     /pseudo
     CDS             complement(1..835)
                     /locus_tag="ACHALG_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
     gene            940..2279
                     /locus_tag="ACHALG_002879"
     CDS             join(940..1381,1381..2279)
                     /locus_tag="ACHALG_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HFN2588174.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYFKKVRNLDSRTRF
                     EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPDKYSKIKQEITAIVK
                     ESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIAKN
                     VLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIISYSISTSPTYKLIEE
                     MLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENF
                     FGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
     gene            complement(2315..>3093)
                     /locus_tag="ACHALG_002880"
     CDS             complement(2315..>3093)
                     /locus_tag="ACHALG_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN2588175.1"
                     /translation="MLDYYYFEKNLSYNYVIHLKTFTGMIFKFANRRYGLKYNPVNGV
                     SLARKPKTNEEIKKEKDGYLSKEEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGEL
                     ISLTESDYDGNKITVSGTYDYELKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKLI
                     KNNKDKYIFISKNGNPISIQAYNQSLRAVSKELNIDKKVSSHMLRHSHISLLTELGIP
                     LKAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
BASE COUNT         1044 a          473 c          526 g         1050 t
ORIGIN      
        1 cggtctgtat gaaaaagctt gacgtctgtt aaagaatacg gtatccgtcc aaaggcttct
       61 tttaccaatg aggcatcttt cttctcacca caggaataac caataatttc tcgattaaac
      121 aaatcaagta ttaagcagat ataatgccac tttttcctca cgcgaacata agtaagatcc
      181 gtaacgatgg cttccaatgg ctgttcttgt gtaaaggtcc gatctaatac attcgctgtt
      241 ttcgcttcat tacaagctgt tcgttgccct ttaaaatgag cgatcgtata ggtagatgtc
      301 aatccgcggc gtttcatgat tcgaccgatt cggcgccgac tgagttgaag cccacgcttt
      361 gctaaacact tcttcaattt tcgggtaccg taagcctttc ggtttcgaat aaactcttcc
      421 tggacgatct cttctaagtc ggactcattt tcgatcggtt tcgcttgata atagtaggtc
      481 tgacgagaaa tatttagaat tttgcacatc gctgatatgg aatatttatg cttgttggca
      541 tcaattactt gtcttttcgt ccgaatatca gcgccgcttg ctttaaaata tcattctcca
      601 tttcgagttg cttatttttc tttcttagtg cgatcaattc tgcttgttct ggtgttaagt
      661 tgtctctttc tttgaatgag cccgttgatt gtgcttgctt catccattta tcgaaagaag
      721 aaggcgtaag ctcatactct cgaataattc ctgcgcgagg cttaccagcg agatagagat
      781 cgacgatttg ttgtttgaat tcttttgaat aggttcttcg ttgacgtctt ggcatgaaaa
      841 atcctccagt gtgtttttta ttattctaca caccttattt tttctgtcta gtctagtgta
      901 gccgattcac tagaaatcta attaaaaggg gtgctttttt tgcggaaata tacatttgtt
      961 tttaagaaaa aagtagtttc agactattta aataacgaag gcggctacaa atatcttgca
     1021 cataaatatc aaataaatcg tacactggtt agacattggg taaggattta taactatcat
     1081 ggttgggaag gcttggttgg aggtggcaaa agctacacta caaaatttaa acttgatgtt
     1141 atagaatata tggaaacaaa tggtctttct atccaagaaa ctgctaaaaa atttaatatc
     1201 ggttcaaata gaactctaag taaatggata gagcaatatg aagaaggcgg tgcttcttca
     1261 cttgagagcc aaaaaagggg cagaaaaatt agtatgaatt ccaagctaaa cattcctaaa
     1321 aaacttaaag atgagtctct tgaagaagaa gttattcgtt taagagcaga gaacgcatat
     1381 ttaaaaaagt tagaaacctt gattcaagaa caagatttga gcaagaagaa atcaaggtta
     1441 aaacaatcct tcagctcaaa aaagaattta aattgaatct attactttca attgcacaat
     1501 tagcgaagtc aacttattat tactgggtaa aaaaattaga taagccagat aaatatagca
     1561 agataaagca agaaattaca gcgattgtaa aagaatcaag aaactcttat ggttatcgta
     1621 gagtcacttt agcgttaaag atgaaaggat atacaatcaa tcataaaaca gttagaaaat
     1681 taatgtccca aatgggactt acctgtcaaa tccgaataaa gcgatataaa tcttataaag
     1741 gaacagtagg aaaaattgcc aagaatgtgt taaaacgaaa tttttcagta gatacaccca
     1801 ataaaaaatg ggtgacagac gtcactgaat tcaagataaa aggaagaaaa atctatctat
     1861 ctcctatact tgatttattt aacggagaaa taattagtta tagtatatca actagtccaa
     1921 catataaact gattgaagaa atgcttcaac aagcaattaa aaaaaaggga actgagggtt
     1981 cactgattct acattcagat caaggatggc agtatcagat gccacaatat caaaaaaaat
     2041 taaaagagaa taacattatt caaagcatgt ctagaaaagg aaattgctta gataattctg
     2101 taatagaaaa tttttttggt gtgcttaaat cagagttttt ttatcgagaa aaattccgat
     2161 caattgagat atttcaaagt aaattaaatg agtatattag gtggtataat aacaaaagga
     2221 taaaactaaa gttaaatgga ctatctccag tagaatatcg gaaacagtcc attaaatagt
     2281 tcaacttttg gggttcagtt caaaaagggg caatttaaat ttctattttt cctagttttt
     2341 ctactagttg attttgcata tttttagttg tatgagtata aatctttaat gttgtttttg
     2401 aatcttcatg tcctacacga tccataatgg cttttaatgg gattcctaat tctgtaagta
     2461 gagaaatgtg ggaatgcctt aacatgtgag aagaaacttt tttatctatg tttaattctt
     2521 tggatactgc tcttaaagat tgattatatg cttgaataga tatcgggtta ccatttttag
     2581 agatgaaaat gtatttatct ttattattct taattaattt gttttcttca attaattgat
     2641 ctatgatttc tttagcatta cttgataatt caatggttct ataagaacct gtatttttag
     2701 ttgttgtttt aatttttaac tcgtaatcat aagtaccaga aaccgtaatt ttattaccat
     2761 cataatcaga ttcagtcaaa gatatcaatt caccataacg tagtccagtt aaaaacaaaa
     2821 atttagtaat taaagaataa cgtgactgtt gatgtgaact tttttgttta ctagcaatca
     2881 tttgaacttc ttcttttgat aaataaccat ctttttcttt ttttatttct tcgtttgtct
     2941 taggttttct tgctaaagaa accccattta cgggattata ttttaatcca tatcgacggt
     3001 tggcgaattt aaaaatcatt cctgtaaaag tttttaaatg tattacataa ttatatgata
     3061 aatttttttc aaaatagtag taatctagca tat
//
LOCUS       DAZABD010000145         3079 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000145 DAZABD010000000
VERSION     DAZABD010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3079
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.152"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..698
                     /locus_tag="ACHALG_002881"
     CDS             <1..698
                     /locus_tag="ACHALG_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS1182-like element ISEfa7 family transposase"
                     /protein_id="HFN2588176.1"
                     /translation="DSQVAFQLVKQSKRLFPEINCVVADAGYKTPKFVHFLTHLNLRP
                     CLPYSRPKGKKGLLSKNEFLYDEYFDCYICPQDQMLAFSTVTREGYREYKSNPKECVN
                     CPLLNQCTISKNHQRVITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKE
                     KHGMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQ
                     ARVTSLEQLLSTV"
     gene            973..2868
                     /gene="asnB"
                     /locus_tag="ACHALG_002882"
     CDS             973..2868
                     /gene="asnB"
                     /locus_tag="ACHALG_002882"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HFN2588177.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT         1059 a          520 c          615 g          885 t
ORIGIN      
        1 atgacagtca agtcgccttt cagctcgtca aacaatcgaa gagacttttt cctgaaatca
       61 attgtgttgt cgctgatgct gggtataaga cacctaagtt tgttcacttt ttgactcatt
      121 tgaaccttcg accttgtttg ccttattcaa ggcccaaagg gaaaaagggg ctattatcaa
      181 aaaatgagtt tctctatgac gagtattttg attgttatat ttgtccgcaa gatcaaatgc
      241 tcgctttttc aacggttact cgagaagggt accgagaata caaatccaat ccaaaagaat
      301 gtgtgaactg tcctctgcta aaccaatgta ccataagtaa aaatcatcaa cgagtcatca
      361 cgagacatgt ttggggagat ctgatggatg aagtggaaca tcttcgccta acagatctga
      421 ataaatcaat ttacaaaaaa cggaaacaaa cgattgaacg aatatttgcg gatgcaaaag
      481 aaaagcatgg gatgcgttgg accaagtatc gaggcttgga aaaagtcgcg acgcatacga
      541 tgctagtgtt tgctgcaatg aatttaaaga aattagccac ctggctatgg aaaggcaagg
      601 agcctttatt tttttgttca aaaattcgaa acgaagtaga taaaaagctg ttccaagctc
      661 gagttacgag tttggaacag cttttgtcta cagtctgaga cacgatcttt aggtcgtgtc
      721 tttttttgct ggtttttttg gaaatagcat ccactaatat ttttttaaca cgaagagaat
      781 aaggtagcct caaaaaagaa aacatggtaa attagatatt gggactttat tttctgtaaa
      841 aacagaaact ttcatctttt gttttcacgt gacgaaattg taagagagaa aatattcgtc
      901 cgctatttat tctatgataa gatgcatatg aatgaaataa acagtaattt tatattagga
      961 tgtgaaagtt aaatgtgcgg tattgtaggt tttgttaacg acaaggacaa caaaaaaaca
     1021 attattaata caatgatgga tcgaatcgtt caccgtggac ctaatagttc aggagaatat
     1081 atcgataaac atgttgcttt aggattcaga agattaagta ttattgactt agagggcggt
     1141 actcagccta tttacaatga agatcggaca aagatcatta tttttaatgg cgaaatctat
     1201 aattaccagc ctttgagaga agaattgatt gcagctggcc acgtttttca aacacatgca
     1261 gatacagaag tccttttgca tggttacgaa gaatggggaa cagaattatt gcaaaagatc
     1321 cgcggaatgt ttgcttttgc tatttgggat aatgaaaaga atgagttatt cggtgcaaga
     1381 gaccactttg ggatcaaacc atattattat gcagaaatga acggaacatt tatgtttggt
     1441 tcagaaatca aaagttttct gcctcatcct gattttaaca aagaattgaa caaagaagcg
     1501 ttgaaacctt atatgacttt ccaatattct ccattaaacg gcgaaacatt cttcaaagat
     1561 gtctatcgtt tgcctgaagg ccattattat acgtataaag atggaaaaat agacattcag
     1621 caatattggg atgcagattt tgaaacaaaa gaaacgcatt cccgacaaga atggatcgaa
     1681 aaaatcgacg aaaccgttca agcttctatt gaagcacata cggtcagtga tgtagaagtt
     1741 ggttctttct tatccagcgg ggttgattcc agttatgtaa cctctgtatt gaaaccagat
     1801 cattctttct caatcgggtt tgatgataaa acctataatg aagcgattga agccagaaaa
     1861 ctaaccgaac tattggactt agataataca gcagcagtga tcgacggaga catgtcgttc
     1921 aaagcattcc ctttgatcca gtatcactta gatgaacctg attcaaatcc ttcttgcgta
     1981 cctttgtact ttttagcaaa tcttgcttca caaagtgtgc gggtagtcca atctggagaa
     2041 ggggcggatg aactgtttgc gggataccaa acgtatggat tccatacgaa ttcaaaattc
     2101 atccgtgtga ttgctcaagg attgaaaaaa ttaccaaaag gaacacgcta taatttagga
     2161 cgtaaaatcg gaaaaatgaa aaacttccat ggacggatcc atttatatga atcacttgct
     2221 cctgcaaaag aatattttat cggtcatgct cgagtgttcg aagaatcaga agcatcagaa
     2281 gtgttaacac caaaatatca aacagcacca tctgtggatg aaatcatgac tgttcattac
     2341 gagaaaacag aaggaatcaa agatgaagtc aataagatgc aatatgtcga tcttcatcaa
     2401 tggatgccaa aagatatctt gttaaaggca gataaacttt ctatggcaag ttctttggaa
     2461 gtccgagtac ctttattgga tatcgaagtc atgaaattgg ctcaacaaat accaagtaag
     2521 tttttattga atcaaaacaa cacaaaagat attttcagac aagctgcgaa caaacatctt
     2581 ccggaagaat ggtcgaatcg tgtgaaatta ggattccctg tgccaatcaa agcttggttg
     2641 aaagaagaac acggctacga acaagtcaaa gcattattcg aagcagattt tgcaaaagaa
     2701 ttttttgatc aagaaaaaat catgaagttg ctagatgacc atcacgaagg acgtaaagaa
     2761 gagcaaagaa aaatctggac aatcttcagt ttcttgacat ggtataaagt ctactttgta
     2821 gatgaaacga ttccgcaagc agaagcaatc gactatgtga ctgtttgatg aaaatcaaat
     2881 atcaataaaa aagaggtttt ctcctaccgt ttaacactga gtgataagaa acaatttcaa
     2941 aaaacagctc ttcccgtttt atagatatta tgacttatgg ttcggagttg gcagaggaac
     3001 accattgttt cgaacataag ggggttgtga cacgattttc gtcacaaccc ctttacatat
     3061 gttcaataat cgatttctt
//
LOCUS       DAZABD010000146         3050 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000146 DAZABD010000000
VERSION     DAZABD010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3050
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.153"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..677)
                     /locus_tag="ACHALG_002883"
     CDS             complement(<1..677)
                     /locus_tag="ACHALG_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN2588178.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVP"
     gene            complement(670..1587)
                     /locus_tag="ACHALG_002884"
     CDS             complement(670..1587)
                     /locus_tag="ACHALG_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN2588179.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(1814..2938)
                     /locus_tag="ACHALG_002885"
     CDS             complement(1814..2938)
                     /locus_tag="ACHALG_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFN2588180.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
BASE COUNT          968 a          574 c          494 g         1014 t
ORIGIN      
        1 aagggaaccg tatatttatc tttatctgaa agcatgacta atggaaatag atattgattc
       61 caacttccca taaagataag taaaccacta gtaataatcg taggtgataa aatcggtaca
      121 gcgattttcg taaaaatcac ccactcattg gcaccatcga ttctggcagc ctccatcagt
      181 gctgtaggaa attgactgct atattgtcgc atcataaaca ctgaagttgt actggcaatc
      241 gaagggatga tcacacctgc ataagtattc gataatccta aacgactcat aatgtaaaat
      301 aaaggaatca atgtggtaaa aaaaggcacc atcctagata gcataactgt attgaacaac
      361 agtttattcc ccataaaacg atactttgct aatgcataac ccgccattgt agaactggct
      421 gtagctaaaa ctgtactgat tacagcaata acaatcgaat tccaaataat caagggataa
      481 ttatatctcg ccatgatggc ttgccagttt tttaatagca tatccccaaa cttgaaattg
      541 attccgccac ttaaaatttc ttgattactt tgcgttgaag ctacaatcat atataaatat
      601 ggcatcaaaa atattaaggc accaaacaca ataatgacgt acgtaaaaat tctgctttgt
      661 atcttttgtt tagtcatttt tatcacctcc gaatttaaat tgaatagccg ataatataac
      721 agatacaaac accatcgtcc aggcaatagc agaggctttt ccaaaatcaa ccatttcaaa
      781 acttgtttta tataaattga ctcctacgac caaagctgaa ttttctggtc cattggaagt
      841 cccaaagaat atatttggga tattgaaatt acccaagcca ttgatagtgg ctaaaactgt
      901 ggccattaaa atgattggct ttagcatggg gatcgttaat aaaaagaatt gccgaaattt
      961 cgatgctcca tctatttcag cactttcgta tatctcttta gaaatccctt gaagaccagc
     1021 taaaaataaa atagtgttat atcctacact aagccaaatc atcgtgatga tcattgatat
     1081 tcgtatccaa aaaggatttg ttaaccactg tacaccgtta attcctagcg cattcaaaaa
     1141 tccattgaat aacccagtcg gattgaaaaa agtcatgaat acggaagcta cagccaccgt
     1201 tgatgtaatg gttggaatat aatagacagt tctaaaaaat ccttttcctt ttaactgtgc
     1261 tgaattcaat aaaacagcaa agtaaatagc taatagcatc gttattggaa ttgatcctgc
     1321 catgataatc aaaacattcg tgtaagattt aataaacaat ggatctcgga agatagctag
     1381 ataattgtca aatccaacaa attcaaatga gtagccaatt ttcataaaac taaaagcaaa
     1441 cgagataatg atgggaatca aaatcgtcac aataaagaaa ataacaaaag gtgtgaccaa
     1501 tccataagca ctttttttat gagacgtatc tgttgctttt ttcttgctgt tctttattgt
     1561 cttgcttggt aacgaagatt gcatcatcgt taagtcctcc taatattttt taatcaccag
     1621 ttaattttac gccgtactta tgagcaaaat tttctgcttc ttttagatat gaattttcaa
     1681 aatcacctgt ttgccaatag ttatatgcag cggtagtcaa ataagacgta gcatctgaat
     1741 aagtggctgg taaaacagta tttgatgtat tttcattgat tttattcaat agttcatagt
     1801 atttttgtcc tccttagtag tcaaactttt tctcagctgc ttctgtttga taggccaatg
     1861 agttagatgt agccacgccc agctccagtg cgttggctaa gcattcttca tctgtcagca
     1921 taaatgtaag aaattgttgt gccacttgtg gatttttggc atctttaggg acataaaaac
     1981 ttgatcctcc tgatactgca ccttttcctg gattttcttt tgaaaaagga atcagtggag
     2041 ccatgcgcca tttgtcagat gcatcaggaa aattcaaaga catattcgta gcataccagc
     2101 cgccagcaac aaacattgca gattcttgaa atgctttctc agcatcttgt gcaccgtaga
     2161 aatgtacaat atctgagtcg atcatttctt tgattaactt tgcagcatgt ttcgcttctt
     2221 tagtattcag acggatattt ccttcttcat caagcaaaga ttgattctgc tgggctagca
     2281 tgttgataaa caattcttgg tccccagatt ctggcaaggc aattccaaaa ctaccagttt
     2341 tttcttttaa tgcttttgcc acctggatat attcttccca agaactgatt tcttcaaaat
     2401 ctacacctat ttcagcaaat ttttttggtt gataataaca aagtgctggg gtaaaatcac
     2461 tagcccaagg aataatcgtt tcattactcg tttgttgacg caaaatattc aatttttgat
     2521 cataaaactg atcgccatat ttgtcataaa atccatattc atcagcacta taaaaagcat
     2581 cttgaaattt ttccagcaca cctgctgctg aagtgtctgc catggagata atatccggca
     2641 tatcttcgtg tgttaccaat tttggggtga tcatttgtac tgttgctgtt cccgaagcta
     2701 cattcacaat ttttaagtca atatttgctc cagttttgcg gttatagatt tttgcagcat
     2761 tttcaacata gtttgtcagt ttgccgtttg cgacatatac ttctaatgta gtggcttttt
     2821 ttatcattgg atctgcgatg tttgctgtta cttcttcgtt agtttttcca catgcactta
     2881 agctgaataa tagggtaatg tttagcaaag ctatcaccaa tttgtatctt tttttcatgt
     2941 ctatcccttc tttcttttca agttcttata taaaacgctt tcacttatgt atattaacgg
     3001 aattagtaac acaaatattt atataaaaaa atattttact tgtcgttttg
//
LOCUS       DAZABD010000147         2662 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000147 DAZABD010000000
VERSION     DAZABD010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2662
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.155"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..792
                     /locus_tag="ACHALG_002886"
     CDS             <1..792
                     /locus_tag="ACHALG_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFN2588181.1"
                     /translation="IPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFDGFSP
                     FPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYFGSIL
                     GINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISFVLSM
                     IGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILLQLSF
                     IGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            862..1602
                     /locus_tag="ACHALG_002887"
     CDS             862..1602
                     /locus_tag="ACHALG_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFN2588182.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            1613..2617
                     /locus_tag="ACHALG_002888"
     CDS             1613..2617
                     /locus_tag="ACHALG_002888"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HFN2588183.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
BASE COUNT          838 a          438 c          544 g          842 t
ORIGIN      
        1 atacctattt ctgaatatcg gaaaaaagca cgagtttctt tggaaaatca atggggaatc
       61 aatacttggc ttgttttctt atctataata tttacgagcg tcatccagaa cttttttgac
      121 ggtttcagtc cttttccgga agaaagcatg cagcagcact tattgagttt tctagtcgat
      181 aatttacttt tattcgcatt caactatgca ttgttttaca ttgcgctagt agttgtacgg
      241 ggtggcagac caaaaagtaa ccttttattt tcagttttcc aaagaaagta ttttggttcg
      301 atacttggca tcaatttgat caattcagtc atcaactggc tactcaacag tttaatcttt
      361 ctgcccatgt ttttaatcag cggagtgagt acttatatgc agctgctttt cagttcaaat
      421 gcggtaccta cgggtgtcat gccagaagat tttcttgata tcagctttgt tttatcgatg
      481 atagggatga tttttgtttc agcactcatc atgagtattg ttggcggttt attccaattt
      541 gctgcatgga caaagttcga ctatcctgat ttgtcggtta ttcaatgttt gaaatatggc
      601 tggtttttat tgaaagatag aattggtacg tatattttgt tgcagctgtc ttttatcggc
      661 tggtatttgc taggagcaat cgctttgttc atcggtcttt tgtgggtcat tgtttatgtg
      721 aatgtgtcag ttgcagaatt ttatgaacaa gcacggattg aaaaaggagg tccagacgcc
      781 tattttactt agcatgtaag cagtttattc atcaaaaaaa taacttttgt ggtaggataa
      841 atctttgagg gggaatgtac gatgaaatta actgttttag gatgcttagg agcgtatcct
      901 tataaaaatc aagggacaac aggttatttg cttcaatctg acgggttcaa tttgttgatc
      961 gatgcaggta gtgctacgct gatcaaatta caagaacacc ttgatccact tgatctagat
     1021 gctgtgatcc ttagccatta tcatcatgat catattgctg accttggtgt attacagtat
     1081 tactggcaac ttcatcctaa gagaaatccg gagacattgc tttcaattta tggacacaca
     1141 aaagattcca ttcactttga agaattgacg ttggaaaatg tctcaaaagg acatccttat
     1201 tttgaagctg aagaattaaa aattggtcct tttctgatta cttttatgga aacaatccat
     1261 ccagtagtat gttacgccat gcgtatcgta gaagaaacaa cggggaaagt attcgtattt
     1321 acaggtgatt caggttattt ggaaagtttc gctgattttg cgaaagatgc tgatttgttt
     1381 ctagcagata cctatctttt tgaaggaaat gaacatcatc atgcccattt cacttcaaaa
     1441 gaagctggag aacttgcgaa atcagctaac gtaaagaaac ttgtacttac acacctacct
     1501 cagtttggtg atttagaaca attgaaaaat gaagcgcaaa aggcagcagg caaagggatt
     1561 gatatagaac ttgctgcagt tgacaaagtg tttaacattt aggaggaaga acatgatttt
     1621 tgtaccaaat gaaaaccacg acccaagaat caatttagca attgaacaat atttgctgca
     1681 agaaatgcct ttagatgaac ctattttgct cttttatatc aatgaacctt caatcatcat
     1741 cggcagaaac caaaatacgc ttgaagaaat caatcgagat tatgtggaag aaaatggcat
     1801 tcatgttgtc cgtcgcttaa gtggaggcgg agctgtctac catgattttg gaaacctgaa
     1861 ctttagtttt atcatgcctg atgatgggga ttcattccgt gactttgcga aagtaacaca
     1921 gccaatcatc caagcacttc atgaattggg tgttgctggg gcggaattga aaggccgaaa
     1981 tgatctagtc attgatggca tgaaattttc tggcaacgcg atgtatgcta ccaacggtcg
     2041 gatgtttgct catggaacaa ttatgttcga ctctgatatc aatgaagtag tcaatgcatt
     2101 aaaagtaaaa aaagacaaaa tcgaatcaaa aggaatcaaa tcgatccgtt ctcgtgtaac
     2161 gaatatcaaa ccatttcttc cagaagaaaa gcaaaaaatg acaacagaag aatttcgtca
     2221 agaaatcttg ttgaaaattt tcggcgtaga gtcagtagaa gaagtaaaaa cgtacgaatt
     2281 gactgatgaa gattggaaaa aaatcaatcg aatctctgag gaatattatc gaaattggga
     2341 ttggaattac gggaagtctc cagatttcaa tttctctcgc caaaaacgtt tttctatcgg
     2401 ttcgattgaa gtccatttga atgtctcaga aggaatgatc aaagaagcaa aaatctttgg
     2461 cgatttcttc ggactaggag atatctctga cgtggaaaaa aaattggttg gacagaaata
     2521 tgataaagca tctctatcaa aaatagtcag ccaaattgat gtgaagaaat attttggtgc
     2581 gatagaacca gaagaattgc ttaccttact ttattgaaaa taaaaaatag cggaatccac
     2641 atatatggat tccgctattt tt
//
LOCUS       DAZABD010000148         2420 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000148 DAZABD010000000
VERSION     DAZABD010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.157"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..287
                     /locus_tag="ACHALG_002889"
     CDS             <1..287
                     /locus_tag="ACHALG_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFN2588184.1"
                     /translation="TQLWFKFDPSNRFIKDFYKVWNSEVFFLAIEDSLLINLYYSNKN
                     YFKIPAAKTRMKKDVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(800..873)
                     /locus_tag="ACHALG_002890"
     tRNA            complement(800..873)
                     /locus_tag="ACHALG_002890"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(837..839),aa:Ile,seq:cat)
     gene            complement(1062..2081)
                     /locus_tag="ACHALG_002891"
     CDS             complement(1062..2081)
                     /locus_tag="ACHALG_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFN2588185.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RSDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            complement(2092..2298)
                     /locus_tag="ACHALG_002892"
     CDS             complement(2092..2298)
                     /locus_tag="ACHALG_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFN2588186.1"
                     /translation="MNNRTFDILKWIAIVVIPALATFVGLVGKGVNWRYTDITVMIIT
                     GFGAFLGSILGVSNRTYKKISSDK"
BASE COUNT          739 a          466 c          404 g          811 t
ORIGIN      
        1 agacacaatt atggtttaaa tttgaccctt cgaatagatt tatcaaagat ttttataaag
       61 tatggaattc agaagttttc tttttagcaa tcgaagatag cttattaatc aatctctact
      121 attctaataa gaactatttc aaaattcctg ctgcgaaaac tagaatgaag aaggatgtat
      181 actttttgtt tgatatcgtg actgacgtgc cggacgcacg tagcgatcat cggcgttatg
      241 actatataaa gtatactttc gttgatccag aaagatacaa agattaaagt aggctaccta
      301 aaaaggtagc ccggaacgaa ttttatcacc atacttgtga aaggagatat tttttaagtt
      361 agtattaaga ttgtgtaata tgatgatatc tatattttat agtatcagtg ctataaaatc
      421 aaaaataagt cactaattaa ctaccactcc aattgtaagc cttttttctc actttttttc
      481 aaaaatatgg tatgcctttt aatagcttca aatataaaag agtttaaagc gtaacacact
      541 tatggggaag tggcttgggg tgcgctttaa actcttcttt attatcatct cacaatttaa
      601 cccaaatgtc tttctattta aaaatcaaag taaaactttt caaatataca gaaatataac
      661 tatgtgaaac atcctttcat taatccataa aaggatacat aaaaaagcca ctcatttgag
      721 tggcaatgaa gaaaagcttt agcttgtata atactcttca aaaaattcta acacagaacg
      781 attcaaatgg ctacgttaat gtaccctgta ggactcgaac ctacgaccgg acggttatga
      841 gccgtctgct ctgaccaact gagctaaggg tactggttgt tgccacataa agccataaac
      901 aatcaaccag tagaatgtgt ggcaacaaac ctgttatcgc atagcttgga gtgtgactat
      961 tatgggtgat agtgaagata tgcgataaca tcactatttt atcgaatgat ttttatagtt
     1021 gtcaatatag ttatgtaatg ctcctcaacg aggagctatt tttatcgttt aggaatattt
     1081 aaataccaac gtttgtcatg gaaatcttgc gcaccgcctt tagtgtttcc ctctggatca
     1141 ttcgttgccc gcatcatgac atagactttc ttattaggga agttacgcat gttaaaagat
     1201 acatgatagc caacatttcc agaagtatta taagcttgat ttacatctga tctataaatt
     1261 ccatcagctc ttactcgagc taattctttc ccagtattgt aatccataat gaagatatac
     1321 tcgtatttat agttagcaat gtgccatcca gctacatgca aatttgcatt ttcgatttct
     1381 ccaaactgat caatgtgggc gtaattcgtt ccatctgtca gtgtaggatt agctgcacca
     1441 gctcgtgttg gatcaatgac aggcttgttt tcagaagttg ttggattttc atcggtaaat
     1501 ccatgagcta aatcatatgc taatttttct ttacttacgc ccatttcaga aagataaccg
     1561 taaggatctg tatgatcgcc ccagatgttt tgtgttaccc ataaatgcga tttgattccc
     1621 ggttggttat aaggagtgtc caacgttaat ggaataccat atttcattgc tgaatctcta
     1681 gccaattcaa cgtatgcctt gtagtttttc tcaaacgttg ctttatcatg tgtgtgttgt
     1741 aactcaatct gcacaggact gttggcatta gcatacgaac cagcaccgta ctgtacataa
     1801 ccaggttgac cgacttgata aacaattccg ccgtctccca caatataagc agtataagcg
     1861 ctagtccatg aacgttgcat atactgcgct tcattgcgtc ctgttgctgt ttcattagcc
     1921 gtttcatgca gtaaaatata ctgattattt gctacttgtg agctaccttc atttgcgccc
     1981 aaattaaatt cattgttgat agtataggca aatccgttaa ttggcaataa aaaaagagcc
     2041 gttaacaggc tcatcgcagt aatagtaatt ttctttttca tatctatcct cctatttatc
     2101 tgatgaaatt ttcttatacg ttcgatttga tacacctaaa atactcccta gaaatgctcc
     2161 aaaacctgta ataatcatca ccgttatatc tgtgtaccgc cagttaacac cttttccgac
     2221 aagaccgaca aaagtcgcta aagcggggat aactactata gcgatccatt tcaaaatatc
     2281 aaaggttcta ttgttcatac tatccacctc ccttcattgg ttgggctact ttctttttca
     2341 atcgcttgtt ttcttctatc aatatttctc tttctgaaag caaggcatca atttgcccgt
     2401 ccagttgttc aattttatct
//
LOCUS       DAZABD010000149         2406 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000149 DAZABD010000000
VERSION     DAZABD010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.158"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..103)
                     /locus_tag="ACHALG_002893"
     CDS             complement(<1..103)
                     /locus_tag="ACHALG_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFN2588187.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQEL"
     gene            complement(135..458)
                     /locus_tag="ACHALG_002894"
     CDS             complement(135..458)
                     /locus_tag="ACHALG_002894"
                     /inference="COORDINATES: protein motif:HMM:NF015824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFN2588188.1"
                     /translation="MDSFQRLSLVNQFELLAKFEDQNRSYYERKIEILREGYEYHYDD
                     EIWSDLSEPFPKEDSRFVLDVLNMYRDINFSKSKLNLDENQITNSYYTHFGSVAKFSK
                     KSILV"
     gene            920..2302
                     /locus_tag="ACHALG_002895"
                     /pseudo
     CDS             920..2302
                     /locus_tag="ACHALG_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          875 a          412 c          400 g          719 t
ORIGIN      
        1 acaattcctg aacttcacga tagcttagat tgtaacgcag gtagtaacca acagcgacaa
       61 taatgacgtc ttttttgaat tgtttgcctt taaaatgatt cattactctg tcctctctgt
      121 cttttttctc aattttacac taaaatagat tttttggaaa actttgcaac agaaccgaaa
      181 tgagtatagt atgaattagt aatttggttt tcatctaagt ttagctttga tttactaaaa
      241 ttgatatctc gatacatgtt caatacatct aatacaaatc gagaatcttc tttcggaaat
      301 ggctcagaaa gatcactcca aatttcatca tcataatgat actcgtaacc ttctctcaat
      361 atttcgattt ttctttcata ataagatctg ttttggtcct caaatttagc caataactca
      421 aattgattta ctagtgacaa tctctgaaaa ctgtccatta aatgtttctt cctatcttgt
      481 tcattaacaa aaaattataa gcccttacaa cgaatattat atattaaatt taattgtagg
      541 tgaacacgaa ttttataaat atttatggtg ttaatgacaa atgatatccg actcctcttc
      601 atacgttctg gactatttgc taatagatta aaatttcagc atctatttct gaccataaat
      661 aaatgagctg ccgaaaagct gccaaatcag acttgaaaat aaaggatcaa aaacttttaa
      721 gtttctgacc ccgtaactca cacttcaaaa tcttacttaa aaaggagtct gaaagaaaaa
      781 aagagtattc aaaatttttt tctaactagc tacatcacat ctatgcattg acactcaaag
      841 aaccagaata agtcatcaga aagcaagggg gtgcggacat aatcctggac gaaaaaataa
      901 acttaggagg aatgtccgtg tgttttccca caaagagcgt gtaagggcaa ttcagttatt
      961 tttaaaatac gattgctctt atgctgcaac cattcgagaa ttagggtatc cgtcgattgg
     1021 tgcactacgg aaatggtata aagagtattt ggaatccgga gagctccatc aggagcaccg
     1081 aaaaaaatca aagtattctg aagaacaaaa acgaatagcg gtgaatcatt attttgaata
     1141 tggtcaatgt tatgcacgaa ccattcgcat gcttggttat cctaatagag agtccttgcg
     1201 gcagcggtgt gaagaacttg caccaggagc tagaaaactc cgaaaaagtg cggtaaagtt
     1261 aacacaagat caaaaagaag ctgtgttgaa aaaattctat aaaccgcaaa ctaataggaa
     1321 aaatttagcc gaatctggag gaattagtag agttactcta tatcaatgga aaaacacggt
     1381 tcttggaaag gattttccat tgtgtatgac tttaaaaaat caggaaaaac aaaaagagct
     1441 acttttaaag gaagtagaag acttacaaaa acaagtacac caattacagc tcgaaaaagc
     1501 attactcgaa ggagcggctg aactgctaaa aaaagaaaaa ggcgtcaatc ttctttgtct
     1561 atcaaatcaa gaaaaaacga tattgattga tgccttacga aatcagttca ccttgaaaga
     1621 gcttcttcaa cagctccagt ttcctaaaag cagctatttt tatcaaaaac aagcacttga
     1681 gaaaccagat aagtattaca aagaacgtca aattaattat cacgattttc aatcataact
     1741 tctgtgcgta tggttaccgt agaattcacc aagctttaaa aaatatgggt aaaaagcttt
     1801 ctgaaaaagt tgtacgtaga ttgatgactg aggaaaatct atttgttaaa ttttctagac
     1861 gaaaaaagta ttcttcgtat gctggggaaa tttctcctgc acaccccaat ttattaaatc
     1921 ggaattttaa agcaaagaag ccaaatgaaa aatggttaac agatattact gaatttagaa
     1981 ttctcgcagg gaaaatctac ttatcaccac ttgtcgattg ttacgatggt gctattgtta
     2041 gttggactat tggaacaaag ccaacttgct tcactctatg tctaaaaaga gttattcacc
     2101 agataattca gcgtgcgaag gtttctttgg acgattaaaa aacgagatgt tttatcaacg
     2161 tgactggaag aatacaacaa tcaatcagtt tataaacaaa ttggatgatt atattcattg
     2221 gtataataat caacgaatta agttatcttt aggaaggcta agccctcttc aatatcggaa
     2281 gaagcaaaga tatatttgtt aaaaaggtcc aaaaaaaata tccgcacccc cttttttttg
     2341 taaaaaacta ttgcacaata caagtagcct acggctatcg aaatttccat aactttcgag
     2401 caagaa
//
LOCUS       DAZABD010000150         2401 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000150 DAZABD010000000
VERSION     DAZABD010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.159"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            41..823
                     /locus_tag="ACHALG_002896"
     CDS             41..823
                     /locus_tag="ACHALG_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN2588189.1"
                     /translation="MKPNPSEAGKRIKQLRLSCGFTMEELGRKIDNSPRATISNWERG
                     TNLPNPQKLKLLSTITNSTIDWIKWGTLEEYITSYLIDIGYELYIKDFPEIPHKVFKD
                     IQERYSNTFSLNKDYELLNPIIKNIFTKYYSKDFEDYRDIEVKPDPKKVGRKIRSIRK
                     KLGLTMQEFGYEVSNSPRSTVSTWEHGGNLPNKAKLKKIADIANCSVEDLLFDEGFIS
                     EKSQVKLINELKEENSKLKEKIKYYECLFDGIESLLNSRKNS"
     gene            863..1474
                     /locus_tag="ACHALG_002897"
     CDS             863..1474
                     /locus_tag="ACHALG_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFN2588190.1"
                     /translation="MLVGYARVSSDDQNLERQLEEFRKIGVEKVFAEKKSGRNTVERP
                     EFNKMLSFVREGDTLVFESLERLGRNSDEILETVSFIDKNGMGLMVLNLPILDTKFGD
                     PNLEKLVRSLVINIFSWTAQNEREEIKRKQKQGIAIAKKKGVYKGKPYEYSSTAKNPQ
                     KRFIYNEIVRMLGEGVPIKRIAEKTGTNRNLVYRIKKRISESD"
     gene            1918..2067
                     /locus_tag="ACHALG_002898"
     CDS             1918..2067
                     /locus_tag="ACHALG_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2588191.1"
                     /translation="MKLEIKEVVCDWGIYVDGETYPFMIFNSKANAQEIMRIMELDNK
                     HERFD"
BASE COUNT          956 a          291 c          461 g          693 t
ORIGIN      
        1 gaatttgaag taacccaaat tccataagag aggtgattgt atgaagccaa atccttcaga
       61 agcaggaaaa cgaatcaaac aattacgcct ttcatgtggt tttactatgg aagaacttgg
      121 aaggaaaata gataattcac cacgtgcgac catatccaac tgggaacgtg ggaccaattt
      181 accaaatcca caaaagctga aattattatc aactatcact aattcaacaa ttgactggat
      241 taaatgggga actttagaag aatatataac ttcatacctt atcgatatag ggtatgagct
      301 atatattaag gactttccag aaatacctca caaagtcttt aaagacattc aagaaagata
      361 tagtaatacg ttttcgctaa ataaagacta tgagttattg aatcctataa taaaaaatat
      421 atttacaaaa tattactcaa aagattttga agattatcga gacatagaag ttaaaccaga
      481 ccctaaaaag gtagggagaa aaattcgttc tattagaaaa aaattaggac ttactatgca
      541 ggaatttggg tacgaggttt ctaattctcc tagaagcact gtgtcaacat gggaacatgg
      601 tggtaactta ccgaacaaag ccaaactaaa aaaaattgca gacattgcga attgtagtgt
      661 agaagatttg ctttttgatg aaggctttat ttctgaaaaa tcccaagtta aattgataaa
      721 tgaactcaaa gaagaaaatt caaagctaaa agaaaaaata aaatactatg aatgtttatt
      781 tgatggtata gagagtctgc tgaactcacg aaaaaattcc tagttaatta aactagagta
      841 aaattatttg taggtgttaa atatgttagt tggctatgca cgagtcagtt cagatgatca
      901 aaatttggaa agacagcttg aagaatttag aaaaattggt gtcgaaaaag tatttgctga
      961 gaaaaaatct ggacgtaaca cagtagaacg gccagagttc aataaaatgt tgtcatttgt
     1021 cagagaaggg gatacgcttg tttttgagtc gcttgaacgc ttaggaagaa attctgatga
     1081 aatactagaa acagtatctt ttatagataa aaatggtatg ggcttaatgg ttttgaattt
     1141 accaatttta gatacaaaat ttggtgatcc aaacttagaa aaacttgtta gaagcttagt
     1201 aattaacatt ttttcttgga cggctcaaaa tgagagggag gaaattaaga gaaaacaaaa
     1261 acaaggaata gcaattgcga agaagaaagg agtctataaa ggaaaaccct acgaatattc
     1321 ttctactgca aagaatccac aaaagaggtt tatctataat gaaatagtaa gaatgcttgg
     1381 agaaggtgtt ccgattaagc gaattgcaga aaaaacagga acaaatagaa atttagtcta
     1441 tagaataaag aaaagaataa gtgaatctga ttgattttaa gttctttgag aaatcaatgt
     1501 tacgagagaa ggattctaag cctgtttcaa acttttaagg tgtacaatta tacaaagaag
     1561 cttctaaaca aaagaattaa ttttgtggca gtcaagtaac gacagaaata gaaaagaaag
     1621 aggctatgcg agagtgaatc aacaagttaa ttgggttaat gattttgaaa aagaatttcg
     1681 actgaaattt tttggaaatc ctacagcaga aagtacagaa gaaaaaaatt atgactggtc
     1741 agaattttag gttgcatatt cgattcaata agtgttgaag ataatagttc tcttacgcca
     1801 cagtatacaa cgttagaata tgacgaaaac agattcaata catttccttg caataccatt
     1861 caagaatcaa ttgataagca tgagtgataa ttgattatct acaggaaggt gttttttatg
     1921 aaactagaga tcaaagaagt tgtctgcgat tggggaattt atgtagacgg agaaacatat
     1981 ccatttatga tttttaatag taaagcaaat gcacaagaga taatgcgtat catggagttg
     2041 gataataagc acgaaagatt tgattaagta aaaaaaatgg agaatgaatg tgaagcagtg
     2101 gaataccaaa aaacatgcgc caataataag tatattttag gtgcaatggg attaggtaaa
     2161 tcagcttcta ttaaacaggt ctacgaagaa tcacaggaga attgcgtata tgaaaattcg
     2221 aattaaagag tgagaatatg tgggagctaa tttatttagt tttatactta ataggaaaat
     2281 caactgaatt tgtatataac aataaggtaa tatttatatt gttcttaata gtttgtatta
     2341 tcgtatttga tgtctatgat ttactaattc ataagaatga aaatagtaat attggtagaa
     2401 t
//
LOCUS       DAZABD010000151         2351 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000151 DAZABD010000000
VERSION     DAZABD010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.160"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..544
                     /locus_tag="ACHALG_002899"
     CDS             <1..544
                     /locus_tag="ACHALG_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFN2588192.1"
                     /translation="ISKENLLNQDFSTETICEKWAADITYIPTKKNGWCYLSSIMDLH
                     TKRIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREVEQWLKTNK
                     IRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYIEGFYNRNRIH
                     SSIHYLTPQEFEELAKEKMA"
     gene            complement(1017..1589)
                     /locus_tag="ACHALG_002900"
     CDS             complement(1017..1589)
                     /locus_tag="ACHALG_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFN2588193.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1605..2210)
                     /gene="fic"
                     /locus_tag="ACHALG_002901"
     CDS             complement(1605..2210)
                     /gene="fic"
                     /locus_tag="ACHALG_002901"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HFN2588194.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          740 a          469 c          359 g          783 t
ORIGIN      
        1 catttcaaaa gagaatctct taaatcagga cttttctact gaaacgatct gtgagaaatg
       61 ggcagctgac attacgtaca ttcctactaa gaaaaatggt tggtgttact tatcttcaat
      121 catggattta cacacgaaaa gaattattag ttatacattt tcaaaacgaa tgactgtgga
      181 ttgtgtcatt caaacattga ataaagcaaa aatacattat cacattccag aaggaatgat
      241 tctacacact gacttgggca gccaatacac agcaagagaa gtggaacaat ggcttaaaac
      301 caacaaaata aggcattctt atagtagaaa aggaacacct tatgataatg ctggaatcga
      361 atctttccat gcctcattga aaaaagaaga agtctacacg actagctact cagattttga
      421 agaagcaaat cgagcgctat ttagctacat tgaaggattt tataaccgaa atcgaatcca
      481 tagctcgatt cactatctaa ccccacagga atttgaagaa ttagcaaaag aaaaaatggc
      541 ttaaccgtct ctactaaaat gtgtccaaga tattgactca aatccagtaa tatcacaata
      601 ggaatgctgt ttattatatt agcagcagtg ctgattttta tgatagttat ttggttctta
      661 aatgaaagat ctaaagacaa tcaacaccgt agggatatgg agataaaatc aatagagtca
      721 atagattatt tatcgataat aacactaaaa aagccacgtt cctaaacgtg acttacgctg
      781 aacgatatgt gttgtttggt agacgaacaa atcgttcatt ataaatatat gatggtatcc
      841 taatatagta agcgtggcta gcataccttt aattacttta ccttttacag ttaccataca
      901 cattttttga gcagaaatca agtaaataaa aaaatattat atttcacaac atgctaatag
      961 actcatatct agaacgcatt aagtatttac ctgtctggtt cgctgagact tcttttttaa
     1021 ctttcttcaa ttttcttctt tattctaaaa agcgtactgc gactgaaacc ggttatagct
     1081 tcaacttcct tatacgattt acctgaagta agcagttcat atgcgtgcct cttcttcggt
     1141 gtgattgttg ctttcggacg tccttctctg aaattaggat tattctttct tgcaaacatt
     1201 tttccttctt gggtacgttc cacaatcata tcacgttcca tttctgctac actcaacaac
     1261 gtccgtgtca ccattcgccc catggacgta ttttcaatcg ttcctagatt caaaaccttg
     1321 atcgttacgt tatcatcgag taaaggttca atgatctcta atgcttctct cgtgttacga
     1381 gcaaatcgat caagcttcgt aacaactatt tcatctccag gcttcagacc agccaacagc
     1441 tgattgaatt ttggccgatc ccgttttgta cccgtaaatt tttctgaaaa aatattcttc
     1501 ttctctacgc cgttctcaag caactgttga atttgttctt ttgtactttg agaagcggtt
     1561 gagacacgtg catatccata tttcgtcatt tggtccacct acttttacaa atcatctatc
     1621 gtatactcag aatacccttc atagaaatag ctgacatcga ttcctttcat atataactcg
     1681 cgatcgtcaa ttttatcagt cagagcattg gaaattagat agcgtatttc caaatcgttt
     1741 atgggacttc gctccattgc agaaagatag tctgctttat cgattaagtt ccaatctaca
     1801 actttttgca attcttcctt taaaatgaga tccagccaaa ttctagtact tcggccattt
     1861 ccttctcgaa atgggtgagc gatattcatc tccacatatt tcttgataat ctcttcaaaa
     1921 gaagtttgtg gcatctgatc aatatgctgt aacgaatgtt ctaaatacat aactggtgca
     1981 aatcgaaaat ttccttttgc aatattgacc gatcggattt ttccggcaaa atcgtaaata
     2041 tccgaaaata gaaattcatg aatttcagat aaacctttaa atgtaccaac ttcgatttca
     2101 tttatttttc cacaatcgta caattctttc gctttttgtt tactgagttt ttcttcaacc
     2161 ttagccaact caacttgatt ggttaatccc aatttattct ctaaaatcat gcgaaatctc
     2221 cttaacttta tgacataagt ttatgaaaca ctctcaaccc cattatatcg cgattttgtt
     2281 tatgtgtcaa aaactattaa gtttctgacc cccgatagat ttttcaagtg tctctatttt
     2341 gcacagtcta g
//
LOCUS       DAZABD010000152         2304 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000152 DAZABD010000000
VERSION     DAZABD010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2304
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.161"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1168)
                     /locus_tag="ACHALG_002902"
     CDS             complement(<1..1168)
                     /locus_tag="ACHALG_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFN2588195.1"
                     /translation="MFKLEKKELEQLGAVITTREICQQPELWEELITFYKKIETELEE
                     FLSKVRSQSKGQRTRVIFTGAGTSQYVGDTLVPYLRKYGDVKNFTFESIGTTDIVAKP
                     LNYLSKEEPTLLVSFARSGNSPESLAAVEIANQVVDTIHHLSITCAEDGKLAKISQKE
                     DDCYLILMPARSNDAGFAMTGSFSCMTLAALLVFDQTDFNQKQKFVCSLIELGKEVVS
                     REEEIKRIVDIDFDRVVYLGSGSLSGLTREAQLKILELTAGEITTIFDSSMGFRHGPK
                     SFVNDKTVVFGFINNDEYTRDYDLDVLEEVRSDKIAAGVYAITQVGKRNFSGNSFEFV
                     KETQLLPDGYLSLAFIMVAQTFALLTSVKVSNKPDTPSPTGTVNRVVKGVTIHEF"
     gene            complement(1181..1909)
                     /locus_tag="ACHALG_002903"
     CDS             complement(1181..1909)
                     /locus_tag="ACHALG_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFN2588196.1"
                     /translation="MEKAFKNKPLYCQLSDLLKERIEVSMIPHDKVPSERELTAQYGV
                     SRTTVRLALKELENIGYIYRRHGKGTFVSGLRKDADLGGLYSFTQQMKNLGRNPETRV
                     LSFDKIKADKFVSHHLNILLGETVFKVTRLRLANCEPLMIEDTYLPAKFFLKLNKQLL
                     QKKPLYDLFVEDFNQTIRLADEELYASLASKDDAKQLIIPEGAPVLHLARQTYNMKNE
                     IIEFTLSVARADQFHYQIRHIRNS"
BASE COUNT          777 a          446 c          287 g          794 t
ORIGIN      
        1 taaattcatg aatagttaca ccttttacaa cacgattaac cgtcccagtg ggagaaggag
       61 tgtctggttt atttgacact ttaaccgatg ttaataaagc aaaagtctgg gcaaccatga
      121 taaatgctaa agataaatat ccatcaggta ataattgtgt ttctttcaca aattcaaaac
      181 tattaccaga gaaattcctt ttgccaactt gagtaattgc ataaaccccc gcagcaattt
      241 tatcagaacg aacttcttct aaaacatcta gatcataatc tctcgtgtac tcatcgttgt
      301 taataaaacc aaatactacc gttttatcat taacaaaaga tttaggcccg tgacgaaatc
      361 ccatagaaga atcaaatatt gttgtgattt caccagcagt cagttccaat atctttagct
      421 gtgcttctct agttaaacct gacaaactac ccgagcctag ataaactact cgatcaaaat
      481 cgatatcaac tattcttttt atctcctctt ctcgagaaac aacttctttt cctaactcta
      541 tcaaactaca aacaaatttt tgtttttgat taaagtctgt ttgatcaaaa actaatagtg
      601 ccgccaaagt catgcaagaa aaactaccag tcattgcaaa tccagcatcg tttgatctag
      661 ctggcataag gattaaataa caatcatctt ctttttggct aattttagct agttttccat
      721 cttcagcaca agtaattgat aagtgatgga tagtatcaac cacttgattg gcaatttcta
      781 ctgcagctaa gctttcagga ctattcccgc tacgagcaaa tgaaaccaat aacgtaggtt
      841 cctctttact taaataattc aatggttttg caacaatatc agtagtacct atactttcaa
      901 aagtaaagtt ttttacatcc ccatatttac gcaaataagg aactaacgta tctccaacat
      961 attgtgaagt tccagcaccg gtaaagatga ctcttgttct ttgccctttt gattgactcc
     1021 taacctttga taaaaactcc tccagttctg tttctatctt tttataaaaa gtaattaact
     1081 cttcccataa ttctggttgt tggcaaatct cacgagttgt aatcactgca cctaattgtt
     1141 ctaattcttt tttttctaac ttaaacacat ctaaaatctc ctaactattt ctaatatgac
     1201 gaatctggta atgaaactga tctgcccgcg ctacacttaa tgtaaattca ataatctcat
     1261 ttttcatatt ataggtttgt ctagctaaat gaagaactgg tgctccttcg ggaataataa
     1321 gctgttttgc atcatctttt gaagccaaac tagcatataa ttcttcatca gctaaacgaa
     1381 tcgtctgatt aaaatcctct acaaataaat cataaagcgg ctttttttgt aaaagttgct
     1441 tattcagttt taaaaaaaac ttagctggta aataagtatc ttcaatcatt aatggttcac
     1501 aattagctag acgcaatcga gttaccttaa aaactgtttc tccaagcaaa atatttaaat
     1561 gatgagaaac aaatttatca gctttaatct tgtcaaatga caaaactcta gtctcgggat
     1621 tcctacctaa atttttcatt tgttgagtaa aactatataa tccacctaaa tctgcatctt
     1681 ttctcaaacc tgaaacaaaa gttccttttc catgacgtct ataaatgtaa ccaatattct
     1741 ctaattcttt taaagccaat ctaactgtag ttcgactaac accatattgt gcagttagtt
     1801 cgcgttcaga cgggacctta tcatgaggaa tcatactaac ttctattctt tctttcaata
     1861 agtcgctaag ttggcaatat aaaggtttat ttttaaaagc cttttccatt agatatctcc
     1921 tatctttcca tccggttata accacaaccc aatgttaaat tggctttttc ccctttgtca
     1981 ataagaattt tatattaaat aaattggggc aacaaacatt tatacaccag tattttcttt
     2041 tatctattta tttcttttta agtaataata actagttttt atttaacttt gataagatta
     2101 ctaaaaaaat taataccact ttgtaacaat tccaaataat attatattca atattcaaaa
     2161 caagctttaa actgatataa acttaaaaaa gcagtaacgt agtggaaaaa ctttcactaa
     2221 atgttttaaa caatctatac aatactttga aattactaga aactagcaac attaagctta
     2281 ttcttataaa gagtatgtcc tttt
//
LOCUS       DAZABD010000153         2270 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000153 DAZABD010000000
VERSION     DAZABD010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.162"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            191..445
                     /locus_tag="ACHALG_002904"
     CDS             191..445
                     /locus_tag="ACHALG_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFN2588197.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            446..763
                     /locus_tag="ACHALG_002905"
     CDS             446..763
                     /locus_tag="ACHALG_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFN2588198.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            1322..2257
                     /locus_tag="ACHALG_002906"
     CDS             1322..2257
                     /locus_tag="ACHALG_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2588199.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNMNITSNKTSRVLLALMG
                     YGILYRDDQNGQILINRAGRSLLMKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRF
                     EVLKIQKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTD
                     VVMITDIYVEDEDIIKYMKPVIKLVNKNKGTRGAS"
BASE COUNT          823 a          307 c          429 g          711 t
ORIGIN      
        1 gatgttaacg gaagtgtatg gtgagtagtt cactcgtatt ttgatatcgt ttgtatatgt
       61 tttcagcgtt ctaagtggct tattttgtcc aataataata tggagtggaa tcccttaaga
      121 aacaccgttg gcaattgttg ttataacatg ttataattta ttcatcaaaa actaaaagga
      181 gtgtgataac atgggaaaag aactaaaggt tagaaagatc ggcaatagcg tgggagtgat
      241 ccttccaagc tctcttggat taaaaagcgg agacattatc caagccaaac aagaaggcaa
      301 cttattcata ttagatacta ctcaaatagc aaaagaacac gatcgcaaat tgattgaaga
      361 aagtttccaa gactttgaga aaggtttaac agtttcagaa attgaaatgg taaaagcttt
      421 cggaaaatac ggctggagtg aatagatgaa agagtacacg ctagtttatt ctaaaagttt
      481 tcagaccagc cttgaaacga taatagaaga atgggaaaat gagcttgctc tttctccagc
      541 cagcataaaa agatttgtag gtgcgatcca gaaggcttta gaactaacca aaaattttcc
      601 aactatgcat gaggaagttt ccgaaatcta tggaatggat atcccaacat atcggatcgt
      661 gatcggcaaa agctatgcct tattttatcg gatcgaccat gaaaaaaaag agatacttgt
      721 aggacgtatt ttccaatcta aacaaatgaa actaggcttt taacaataat taggaaaaag
      781 tcgctcatcc tctacaggtg ggtgattaat gccgctcgat atggttgtta accgtcggtt
      841 actcgaagat tgatatataa gacttagtac ctttttattt tgctccatgt tatggccgaa
      901 ggtctaaaga tacaatcatt agtctagaga aaaattaaga accgtaggaa ctacggggat
      961 cgcttggtga ataagagaca ctgctattag tgaaaaaata agatatcaag tacgctctat
     1021 tcccaaaaat ttcgtgactt tactcatgag aggttcaagt agtatatttt taatcaaaat
     1081 tgacgtaact tttaataaat actacctaaa taaggtagta tttatttttg tgttttaaaa
     1141 gaggaagtcc aatatatcta tgggaaactt cttaaatatt atgttggtac atgaaaatat
     1201 acatccctgc tagactattt acaacatttt actttgtacg tattcttgtt ttaaaaggaa
     1261 tgggataaaa tataggtaca ataactattt gatgctaaat ataattaatg gaggagagat
     1321 agtgaacata cttaatgaaa aaagatatgt atggtttgct ggtaaaaaat atattactac
     1381 tttcaaaaga ttatccgagt taatgcagca gactgttaaa agtgctatgc ctgaaggata
     1441 caagtttaat tttgttcaat cttcagtatc tactgaaagt atctattttt caattctttc
     1501 tgagagtagg ctttttcatt ttagaataag ttctcatgta aagaaaactg atgttgatat
     1561 atatacgttc cgtacaaata attataaaaa tttaaatgat ttgagtagaa gtatcagtga
     1621 atatctcaag gggaatagct atctcaagat tcgattttca catatattaa ttttactacc
     1681 atatttgaat gagaatttca ccctaattca agtagataag tctttcattg cctcaggtaa
     1741 tcaaggaaat atgaatatta caagtaacaa aacgtcacgt gtgctattgg cgttgatggg
     1801 ttacggaatt ctttatagag atgaccaaaa tggccagatt ctaataaatc gggctgggcg
     1861 ttcattatta atgaaatata gaaaatttat accggaagat tttcaaaact tacagttaga
     1921 aaacatgcgt ttagaagatt tatttagatt agcagaagat gataagtata gatttgaagt
     1981 tttgaaaatt caaaagaatg aactattgaa taagttatat cctgagaatg cgataaaagc
     2041 acattttggt gatgctaatc atatgtatag ttggttcatt cctaaaagat ttagagggaa
     2101 aatcaatgct ggagatcaag tgaaagtact caatcgaaat agtacagatg tcgtaatgat
     2161 aacagatatc tatgtagaag atgaagatat aatcaaatat atgaagcctg tgataaaact
     2221 tgttaataaa aataaaggaa cccgtggtgc tagttaattt tttcctagag
//
LOCUS       DAZABD010000154         2230 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000154 DAZABD010000000
VERSION     DAZABD010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.163"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..376
                     /locus_tag="ACHALG_002907"
     CDS             <1..376
                     /locus_tag="ACHALG_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFN2588200.1"
                     /translation="NVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFRR
                     FPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTDD
                     WQKVMAAEVIATYLDGDLVYKK"
     gene            424..1824
                     /locus_tag="ACHALG_002908"
     CDS             424..1824
                     /locus_tag="ACHALG_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFN2588201.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            1857..2024
                     /locus_tag="ACHALG_002909"
     CDS             1857..2024
                     /locus_tag="ACHALG_002909"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2588202.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAFTGSVAKF"
     gene            2078..>2230
                     /locus_tag="ACHALG_002910"
     CDS             2078..>2230
                     /locus_tag="ACHALG_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFN2588203.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVREILYDRGINVSH
                     TTIYRWV"
BASE COUNT          626 a          371 c          483 g          750 t
ORIGIN      
        1 aaatgtttcc acagaggact tctttaatta ttgtcgatta gaggaaagtg gtgtaacagt
       61 cactagcggt acagatttac cgctgtttat tactgatatt cctgaatcga ttataagagc
      121 agtattccgt cgttttcctg aaggagaaga agtctgggag aaagaaacaa gcttcacgac
      181 aaccgagctg ctgaaaagtt ttacaagcaa cagcttcaaa gtaaatggtt tatcggagta
      241 cggagaaata aaagtaggga aaagcgccag cttagctgtt ttcagcgagg acctctgtac
      301 tgatgactgg caaaaagtga tggctgcgga agttatcgct acctatcttg atggtgactt
      361 agtttacaag aaataatgct tttcgatata acgttataaa acgaattaaa gagaggttaa
      421 aaaatggaaa caatgaaaag aaaattcacc ttcccacata gttatacatt gatttttatc
      481 ttaattattt tggctgcaat tttaacttgg ataattccta gtggtcagtt tgagacagtt
      541 gttgaaaacg tcgcaggtat ggaaaaaaca acggttgttc cgggaaccta tcaccaaatc
      601 gataaggcag attatggaca aggtttttcc tctatactaa tggcaccagc tgaagggatt
      661 atttcagctg ttgaggttgt tgcttttgtt ctcatcgttg gtggtgcctt cgggattatc
      721 ctaaaaacgg gagccatcga tcgagggctg tttaccttag caaaagttct gggagacaag
      781 gggatcttag ttattccgat gtcgatgatt ctattcagct taggaggatc aacctttggc
      841 atgagcgagg aagtcattcc cttatttgcg gtgtttattt ctctgatgtt ttctcttggc
      901 tatgattcca tgactgcgat cttgattcta tttttaagta cacaagcggg atatgttggg
      961 tcgacgatta atcccttcaa cgttttaatc gcacagggag tagctggcgt ttacggaaat
     1021 cccctgcttt ggtttcggat gctatcctgg attgtcatta ctgggatttc aattgttttc
     1081 accatgcttt atgctagcag agtcaaaaag aatccactgt cctctattgt ttatgagagt
     1141 gatttaaagg ttaagcataa gtttcaaaat tttgacactg aaaaagagtt ttcaacacaa
     1201 gataaactaa ttatcgccgg ttttatcgta ggtttaggaa tcatgatttg gggtatcatt
     1261 tccaagggat ggtatatggt tgaaatttct gcactattct taggattagg tctttttgct
     1321 gggggcatcg ctagattttc tcaaaaggaa attgctgaag cttttgtcga aggctgttca
     1381 gatttcgcct atgctgctgt aatcattggg ctagctagag gagtcttggt aatcttggaa
     1441 ggtggaatga ttattgacac tattctaaac agcttggcta accttttagg tggtcttcca
     1501 aaattcctgt tcactacgat tctactcatt tctcaagtaa ttgtttcctt ttttgttcca
     1561 tcctcctcag gagctgcggc attaacgatg ccggttatgg caccattggg tgatctaatg
     1621 aatgtaagtc gtgatgttgt tgttctaagt aaccagtttg gaaatggttt aatgaatatt
     1681 atctccccaa ctagtggtgt attactggca ggattagcca tcgcagagat taatttctct
     1741 aaatggttta aggttggctt caaaatattt gccatcttaa cccttgtttc tgctgttttg
     1801 ttatacattg ctacactctt ttagtcttaa tcatccatta ataggaggca atagttatga
     1861 ttaaaataaa tcaattagca ggaatgaata tccactattt attttattct ctcaaagaat
     1921 ttttcgctta tcaacagaaa attggtattt cttctgttga actttgggga ggtgctccgc
     1981 attttttcat agatgctttt actggttctg ttgcaaagtt ttaaatctac tatcaaataa
     2041 ggtagaataa tagaaaaaga tagcaggagg aatgacgatg aatcatttta aaggaaagca
     2101 atttcagcag gatgtgatta ttgtagccgt gggctactat cttcgttata accttagcta
     2161 tcgtgaagtt cgagaaatct tatatgatcg tggcattaac gtttctcata cgacgattta
     2221 tcgttgggtg
//
LOCUS       DAZABD010000155         2164 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000155 DAZABD010000000
VERSION     DAZABD010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.164"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            214..1188
                     /gene="bsh"
                     /locus_tag="ACHALG_002911"
     CDS             214..1188
                     /gene="bsh"
                     /locus_tag="ACHALG_002911"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFN2588204.1"
                     /translation="MCTSITYVTRDHYFGRNFDYELSYNEVVTITPRNYKLVFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKKIQKEKDNVSPFEFI
                     PWILGQCSTVDEAKNLLTSINLANINYSEELPLSPLHWLLADKEKSIVIESTKDGLHI
                     YNNPVGVLTNNPSFDYQLFNLNNYRVLSSEIPKNNFSDNIDLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKMNSVSGDSESESISQFFHILGSVYQQKGLCDVGEGKYEYTIYSSC
                     CNVDKGIYYYRTYENSQITAVNMNKEDLNSRSLIIYPIVENQQIKYIN"
     gene            1481..1849
                     /locus_tag="ACHALG_002912"
     CDS             1481..1849
                     /locus_tag="ACHALG_002912"
                     /inference="COORDINATES: protein motif:HMM:NF026041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFN2588205.1"
                     /translation="MNHCTRKLLGLTDEHLFFEEEWLETVEEEGVRTNLIHANLSYIP
                     SHCRKCGIKNEGQIIKNGSHKTKVQLLPYRATKTELRLVRTRFYCKECQSTFNAQTNL
                     VDENCYLSKKLKKLSVIHKF"
BASE COUNT          799 a          315 c          344 g          706 t
ORIGIN      
        1 gcagcaacga ttttatctaa aactttttct tctttatttt ctaaattcga catttatctt
       61 ctctcccttt tttatgataa tttagtaata aaactttaat atgatttata caaagaaagc
      121 gctcttttta tacggggccc ttattagttg atatgtgaga acgttaggag tactatgaat
      181 ttgaacaaac taatatttta ggaggaatat tgtatgtgta cgtctattac ttatgtaaca
      241 agagatcatt attttggaag aaattttgat tacgaactat cttacaatga agtagtcacc
      301 attactccaa gaaattataa gttagttttt cgaaaggtca atgatttgga tacacattat
      361 gcaatgattg gtattgccgc tggtatcgct gactaccctc tttattatga tgcaacaaat
      421 gaaaagggct tgagtatggc tggactaaat ttttctgggt atgcagatta taaaaaaata
      481 caaaaagaaa aagataatgt ctccccgttc gaattcattc catggatttt aggccaatgt
      541 tcaactgtag atgaagctaa aaatttatta acttctatca atttggccaa tataaattat
      601 agtgaagaac ttccattatc tcctttgcat tggttattag cggataaaga aaaatcgatt
      661 gtaattgaaa gtacaaaaga tggtcttcat atatacaata atcccgtagg cgttcttaca
      721 aataaccctt catttgacta tcaactattt aatttaaaca attatcgtgt attatcgagt
      781 gaaattccta aaaataattt ttcagataat attgatctga atgcttatag ccgcggtatg
      841 ggtgggatag gattgcccgg agatttatcc tcagtctctc gttttgttaa agcaactttt
      901 acaaaaatga actcagtatc cggggattca gaatcagaaa gcattagtca gtttttccat
      961 atcttaggtt cagtatatca acagaaaggt ttatgtgatg ttggtgaagg taaatatgaa
     1021 tacacaattt attcttcttg ttgcaatgtt gataaaggaa tttattatta tcgcacatat
     1081 gaaaatagtc aaattactgc agtgaatatg aataaagaag acttaaacag tcgcagttta
     1141 attatctatc cgatagtaga aaatcaacaa attaaatata taaattagta gtgaattgtg
     1201 atttataaat agtatttaaa tttaacaaaa aaggaaatat acaaaaactt ccttgaatct
     1261 acctcaataa gatgaaatga tgagacaaaa taaaaagcat cccttgaata tagtgaaccc
     1321 aaaaaggtag aattttatga gttatacaat atttagtgtt agtggaaaat ctatggattt
     1381 tctattgaca cttttttgta aaaaaagata gaaaaagaga tagcctcact ggtagaattt
     1441 agccgtctaa aacaaaacca aaccaagaaa ggatatctcc atgaatcatt gtactagaaa
     1501 actgttaggt ttaacagatg aacatttatt cttcgaagaa gaatggttag agactgtaga
     1561 agaagaggga gttcgcacca atcttatcca tgccaatctt agctatatac caagccattg
     1621 ccgtaaatgt ggcatcaaaa acgaaggaca aatcatcaag aatggctcac ataaaacaaa
     1681 agtccaactt ttgccttatc gagcaacaaa aacagaatta cgtttggtac gtacgcgctt
     1741 ttactgtaaa gaatgtcagt cgacattcaa cgctcagacc aatttggtgg atgaaaactg
     1801 ctatctttca aagaaattaa aaaaactttc agtaatacat aagttctaga ttttttaatc
     1861 attcactcta tccatgaaac ctttaatcgt tttatattac aaaatgttag aaaattcaag
     1921 catttttatt cttgaaaacc aataaatgga tcacacataa tatttacata aagtaaaaag
     1981 gagaagtaat acatatgtat cccaccaaat atccaattta tccgactttt cgatctcaaa
     2041 ataaaaaatt cattttcatt ctaaaaatag aattgataaa tcagttgaaa aaaggatttt
     2101 aatttttata attaagttag ggatatccca gtgtcacttt gggggtaatt taggggaaga
     2161 agtt
//
LOCUS       DAZABD010000156         2061 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000156 DAZABD010000000
VERSION     DAZABD010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.166"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..729
                     /locus_tag="ACHALG_002913"
     CDS             <1..729
                     /locus_tag="ACHALG_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFN2588206.1"
                     /translation="SILLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEPKSIPTTLNV
                     ALIGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGMNKINAAYSY
                     GGVDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSLDLDGVYINK
                     GEQVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFKLPKAVGKLL
                     GSIQTNLPESVLLDCGMDFLKDIC"
     gene            787..>2061
                     /locus_tag="ACHALG_002914"
     CDS             787..>2061
                     /locus_tag="ACHALG_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NTF2-like N-terminal transpeptidase
                     domain-containing protein"
                     /protein_id="HFN2588207.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGWKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFK"
BASE COUNT          740 a          363 c          451 g          507 t
ORIGIN      
        1 tctattttgt tactgcttac attagtagtt ggctattttt cgattgagtt tatccatgga
       61 ttttcgtctg caaaacagag ttcaactgtg aaaaaggtag agccgaaaag tatccctacc
      121 acactaaatg tggctttgat tggttcagat gctcgttcga aagacgaaaa cggtcgctcg
      181 gattcacttc tggttgcaca atacaaccag aaaacacaac aagcaaaact gatctctatc
      241 atgagagatt catatgtcga tattccaggt tacggaatga ataagataaa tgcagcttac
      301 tcttatgggg gagttgattt attaaatcaa acattaaagg aaaatttcaa atttgaagct
      361 ccctattatg caagtatcac atttcaagat tttatcgatt gcgttaatga actgtttcct
      421 ggtggagtca agatcgatgc agaaaaatct ctagatttag atggcgtata tataaacaaa
      481 ggagagcaag tgatggatgg taatacttta ctccagtatg ctcgatttcg ggaagacaaa
      541 gaaggagact tcgggaggat cagaaggcag cagcaagtga tcaaagctat ctctcagcag
      601 ctgaaagacg taacttcaat attcaagcta cccaaagcgg ttgggaaatt acttggtagt
      661 atccaaacga accttccaga aagtgtgctt ctggattgtg gtatggattt cctcaaagac
      721 atatgttaga ataaacaggt ataaatagtg aaataaagga atgacaagca agagaaggag
      781 gaaaaaatga aaagaagtga caagcacggc aaaaatcgaa caggcgctta tattgccggc
      841 gcagtgatct taatagcagc tgcgggtggc ggttattttt actaccagca ctatcaagaa
      901 acccaagcag tagaagctgg agaaaagaca gttgagcaat ttgtccaagc tttgaacaag
      961 ggagattata acaaagctgc agaaatgaca tcaaaaaagg cggcaaataa aagtgcatta
     1021 tctgaaaaag agatcttaga taaataccag aatatatacg gtgctgctga tgtcaaagga
     1081 cttcagatat cgaatctaaa agtagataaa aaagacgatt ctacttacag tttttcatat
     1141 aaagcaaaga tgaatacctc attaggtgaa ttgaaagatc tttcttataa aggaacatta
     1201 gacagaaatg atggtcaaac cacgatcaac tggcaaccca acttggtttt tccagaaatg
     1261 gaaggaaatg acaaagtaag cctgaccaca caagaagcag caagagggaa tattatagat
     1321 cgaaatggtg aaccattagc aacaacaggc aaactaaaac aattaggagt cgttccaagt
     1381 aaacttggag atggaggcga aaaaacagcc aatatcaaag cgattgcttc ctccttcgac
     1441 ttaacagaag atgctatcaa tcaggcgatt tcacaaagct gggtacaacc cgattacttt
     1501 gtcccattga aaatcattga tggagcaacg ccagaacttc cagctggagc taccattcaa
     1561 gaagtagacg gcagatatta tcctttgggt gaagcagctg ctcagctgat tggttacgtg
     1621 ggagatatca cagcagaaga tattgataag aatccagaat taagcagtaa tggaaaaatc
     1681 ggtcgctcag gtttggagat ggcttttgat aaggatcttc gtgggacaac aggttggaaa
     1741 ttaagcatta cagatgcaga cggtgtagag aaaaaagttc tgatcgaaca tgaagttcaa
     1801 aatggcaaag atatcaaatt gacaatcgat gcgaaggcac aaaaaacagc ttttgacagt
     1861 ctaggaggaa aagctggatc aactgttgcg acaacgccaa aaaccggtga tcttcttgcg
     1921 cttgctagct ctccaagcta tgatccaaac aaaatgacaa acgggatctc acaagaagat
     1981 tacaaagctt atgaagaaaa tcctgaacat ccattcatca gccgatttgc gacaggttat
     2041 gctcctggat ctacgtttaa a
//
LOCUS       DAZABD010000157         1994 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000157 DAZABD010000000
VERSION     DAZABD010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.167"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..424)
                     /locus_tag="ACHALG_002915"
     CDS             complement(<1..424)
                     /locus_tag="ACHALG_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFN2588208.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQI"
     gene            complement(533..1564)
                     /locus_tag="ACHALG_002916"
     CDS             complement(533..1564)
                     /locus_tag="ACHALG_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN2588209.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT          615 a          328 c          345 g          706 t
ORIGIN      
        1 gaatctgttt tgattttgtt ccatttgaaa tttctctcac tcgctttttt gtctcatcat
       61 caaataaaat gaaagggtct tctaaacgat cgataaatac ttccattgtt gttgttggta
      121 gatgataaga agtcatgaca ggcactgcac caaggtaaga cacagaaaca gctagaaggt
      181 atgtgtcaaa agcggaactc ttataaataa taactttgtc tccggatttc actccaagat
      241 gagccaattg ataagcccgt ttcaacacac tttcgcagca ttttttgtaa gttgtttcaa
      301 gtcctaattc tgggaaagct gaaagtggct catcaaagat aatcggggtc tcggaaaagt
      361 tagctgcagc ttcttgaaaa ttcgtaaata agttcaaagg ggaatacgtt atggtctcca
      421 tcattcacac tccattatct tttgttaagt aatatacaaa ttcaagataa tcgtaacata
      481 atcctttata taataacata gtttattgtg aaaaaaagat catacatttg ttttatatgg
      541 aagaaacccg aacgaatgcc gctaattcgt gataagaaaa atcaaccatc ttttcccaga
      601 tatcttgaaa agaatgggat gagaggtatc gattaccaca taaataattt accgcgtcag
      661 catcatctaa atatagttct gcataataac gatatgtttt tctgcatagt tctggcgata
      721 cgtttttata tactgtaatt ttgctcaaaa tttggcgaat agtccgagga gaaatttgct
      781 gatcatgtga ggaacgaaaa acaaaagaat tttcgtccgt attttgtgct gttaacttaa
      841 aatagttctc cagaaatggc atcgcttctt ttggtattgg tactttattc agtctgtttt
      901 cttttctatt cagattaaga atctgcttgg tcaaagatac atctttacgt ttaagttcta
      961 ctacttcatg gatctttaat ccgcaataca gaagaatggc agtaattgca aggttcctcg
     1021 gtctattctt ttcttcaaag ctagcttctc tgcttgtttg aaagccttca atcgggataa
     1081 acaaatgctc aaaatctttg aaaagttctg catcgatata tcgcggagct gctttggttg
     1141 tttccggtaa acttgtaccc aaaagagaaa tctcttcttc aatcagtcct ttttgataca
     1201 aaaatcgtaa aaaatgattt aatgaatatt gcttccttga aatcccgtaa tagctatttt
     1261 caaacaattg ctccgtttct tggttcgctg tagtttggaa cgaacgagta tgtcgataga
     1321 ttttagctaa gaaattctga taatcttgtt tttctaaatg gataatcagc tgatcatcca
     1381 cttcttcaga aaatactttt ctcaaataat cagtaaacag atgaaaatct tttgcgtatt
     1441 catagcgact gcctaatgaa agggaagaac gattttcttc taaaaactgt tgaaagacgg
     1501 aagggagcaa gtgtgtacgt tgctttaact tttcttgcag ttgttttcta gttttgctgt
     1561 ccattacaaa ttccctccaa tagtgttcat tttctatatt ttaccgcatt tcatcaaatg
     1621 aagatagaca gtttgtcttg atcttaggaa gcaaattttt aactaagtac tagctaaaag
     1681 atatgaagta agaagataca tttttagtta ttttattagt gcagtcattc ttttgatctt
     1741 ttaattgatt tatcctcttt ttagctatgt ggttacttca tgagcaatga aatggatttg
     1801 gttcgttcta atggcaggag gctctttctt ttatttccga aaaattagaa aacatttttt
     1861 cagaatcgat tggctctcca tttattcaat aatggtttgt ctaacgatat tgttgggata
     1921 attcttaaag aagtattaga aatagttggt acttcattac tttacatgaa gatttattct
     1981 atggtaggag caat
//
LOCUS       DAZABD010000158         1845 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000158 DAZABD010000000
VERSION     DAZABD010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1845
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.168"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            282..407
                     /locus_tag="ACHALG_002917"
     CDS             282..407
                     /locus_tag="ACHALG_002917"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2588210.1"
                     /translation="MHLHRISALAVQIGRYHHSETRAVMVTRCCLLALVIAAANA"
     gene            589..1751
                     /locus_tag="ACHALG_002918"
     CDS             join(589..865,865..1751)
                     /locus_tag="ACHALG_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFN2588211.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKW
                     VKQSKTSGSFKEKDNPTPEQKELLELRKRNQQLEMENDILKQAALIFGPKRQVIDANK
                     HLYPISAMCRILGLSRQSYYYQSKPKKDESELEEAVAEEFIRSRKAHGSRKIKKALSK
                     RGIQISRRKISRIMKNRGLKSSYTVAYFKVHHSTCNEAKTTNVLNRKFLRDNPLEAIV
                     TDLTYVRVGKKWNYVCFILDLFNREILGYSCGEHKDAVLVKKAFSRIKQPLTEVEIFH
                     TDRGKEFDNQAIDELLTTFDINQSLSHKGCPFDNAVAESTYKSLKVEFVYQYTFETLQ
                     QLDLELFDYVNWWNHLRLHGTLGYETPVGYRNQRLAQRILDNELGCANASEAV"
BASE COUNT          626 a          356 c          351 g          512 t
ORIGIN      
        1 attagtagat taatacactt gttttttaga attgtaaata actcataaaa aatattttga
       61 ctttcaaaat cacttttgct accagaagtt gaggcgaagt gttttaagca tagttgtacc
      121 ctagtttgac cgttaaaaat aaataacgat cgctagagtg caactatgtt tgtttgccct
      181 accgtcagcg tcggcttggc ctctcacatc cattaccgtc ggaattatct ccgttacata
      241 cccttacaaa atacgccctg taagcattgc atgaaaccac cgtgcatttg caccgtattt
      301 ccgccctagc cgttcaaatc ggtcgctacc accattcaga aacacgtgcg gtaatggtta
      361 cacgttgctg tctcctagca ttagttattg cagcggctaa tgcttaacct ttctacttta
      421 tcgataattt aatatcaggc agcttggctc ctacctctga tactattcgt atgtcttaca
      481 aacagaatcg taccaagctt tatattagtt ttatggataa gctacaatta actagacaga
      541 aaaattaagg tgtgtagact agaagaaaac ataccaggag gaatttttat gtctaagaga
      601 acacgaagaa ctttttcaca agaattcaag caacaaatcg tcaatcttta cttagctgga
      661 aagccacgtg tagaaatcat tcgagaatat gaactaacgg cttcagcatt tgacaaatgg
      721 gtaaagcaat ctaaaacgag tggttcattc aaagaaaaag ataatcctac gcctgaacaa
      781 aaagaattgt tagaactacg taaaagaaac cagcaattag aaatggaaaa tgatatttta
      841 aagcaagcag cgctgatatt cggacgaaga gacaagtaat cgatgcgaat aagcatcttt
      901 accctatatc agcgatgtgc agaatattag gtctatcacg tcagtcctat tattatcaat
      961 caaaaccaaa gaaagacgaa tcagaacttg aagaagcagt cgctgaagaa tttatccgca
     1021 gccgaaaggc ccacggctca agaaaaataa aaaaagcctt atcaaaacga ggcattcaga
     1081 tcagccgacg aaaaattagt agaatcatga aaaatagagg attaaaatcg agctatactg
     1141 ttgcttattt taaagtacat cattctactt gcaatgaagc caaaacgaca aacgtattga
     1201 atcgtaaatt cttaagagac aacccattag aagcgatcgt aacagacttg acttatgtac
     1261 gagtcgggaa aaaatggaat tatgtctgtt tcattttgga tttgttcaat cgagaaattc
     1321 tcggctattc ttgtggagaa cataaagatg ccgttctagt aaaaaaagca tttagccgta
     1381 tcaaacaacc tctgacagag gttgagattt ttcatactga tcgtggaaaa gagtttgata
     1441 accaagctat tgatgaatta ttaacaactt ttgacatcaa tcaatcattg agtcataaag
     1501 gctgtccttt tgataatgcc gtagctgaat caacttataa gtcgctgaaa gtagaatttg
     1561 tctatcaata cacatttgaa accttacaac aattggattt ggagttattt gactatgtca
     1621 attggtggaa ccaccttcgg ttgcacggta cacttggcta cgagacaccg gttggttacc
     1681 gtaaccagag attggcgcag cgaatccttg ataatgagct cggatgtgct aacgctagcg
     1741 aggcagtcta actttaactg ttagctcctg ccgaagatcg tcacatccga ggaggctcat
     1801 tgtcaaggac aatcggaata gcatacggaa agaagttctg cacct
//
LOCUS       DAZABD010000159         1770 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000159 DAZABD010000000
VERSION     DAZABD010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1770
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.169"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1114..>1770
                     /locus_tag="ACHALG_002919"
     CDS             1114..>1770
                     /locus_tag="ACHALG_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HFN2588212.1"
                     /translation="MNDSIKKMLRIIEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWKAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     V"
BASE COUNT          629 a          288 c          280 g          573 t
ORIGIN      
        1 aaaaatcgat caaattgttg aaaatgaatc tatagaagat tattatatcg aactatcaca
       61 caatgattcc gattctttaa atcactacaa actaataaaa tattcctaac cacagcaact
      121 attttgatta acctttttct gaggggacat ttcatctaaa gtgaaactat cacttcaagt
      181 gttgactaat taagtattta attcactatg atagcgtcaa aaatttattg gacaacagct
      241 tcctccaaaa tttaatttta ttagatgttg actcgttaag gagtctttac tgagtaaagc
      301 tttggctatg cgcttattta gtctaataac tattccattt tccaataaag aaaccgttaa
      361 ttgaatgatt tttaggatta ataaatctcc atagcgtttt aaaactttca attgttctat
      421 atttttaatt atagttaaag tactttatac atacaaaaat taaactaaat gaaaggaatc
      481 ctgtaatgaa gctaatccca tacaatcaat catttcaatc taatattgag aactaccatt
      541 taaaagattt gtcatatacc gcacttccca aagaaattct caagagttca ccagaaactt
      601 ttttcctgta ctttgttttg agaaagatca attagtctgt ttctttgttt tagatagcgg
      661 gaaagataag tttaaataca caaaagatgc taatagctta ttacttaggg cattttcaac
      721 caattatttc caaacaaaaa aaggatttac cacttctgca ttaaaaaaat taccaacatt
      781 tactaaaaga acttttctgg caataaatag tatctattta ggtgtaaata taaaaaataa
      841 attagccatt catctatatg aaaaagtagg ctgtgacata agtctataaa aaacaccaga
      901 aatggacggt tttccgtcat ttctggtgtt ttttgggtaa aataaaagag tacggtagca
      961 gccgtactct aaatcatggc tctataattt atgggactga tgaatcagaa ttgattcttc
     1021 agccccattt tcattttagg tattaaaaaa catatcgttc tctagtatga ttaaatcacc
     1081 acaaaataaa caactgagag gacgatatgc tatatgaatg attctatcaa aaaaatgctg
     1141 agaataatag agaaagattt gatgattaca gaggtctctt acgagaccct tcagaagaaa
     1201 aagacgttgg tcgtcgatgc tgttctctcg cctactcctc gtgcttgtag aagttgtggt
     1261 tctactgtgg tagatggaaa cgggaaagca attatagtga aaaatgggaa gaaggaaacg
     1321 attgtccgtt ttgaacaata caatcatatg cctttggtta tgcgcctaaa aaagcagcgc
     1381 tatacctgta aaaactgccg aacccattgg aaggctcaaa gttattttgt ccaacccaga
     1441 cattcaatcg caaatcatgt tagatataaa attgcttctt tactgactga aaaagtatct
     1501 ttatctttta ttgcgaaaag ctgtcaggta tctttgacca ccgttattcg tacattgaaa
     1561 gagtttaaaa gctatttacc aaagcaatct aagaagattc tccccagagt attgatggtt
     1621 gatgaatttc gttcgcatgc ttctatagaa gataaaatga gctttatttg tgcagatggc
     1681 gaaacaggaa aattaataga tgttttgcct acgcgtaaat tacctcgatt aacaagctat
     1741 ttcttaggtt gtaccaatcc agaagaagta
//
LOCUS       DAZABD010000160         1762 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000160 DAZABD010000000
VERSION     DAZABD010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.170"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(149..964)
                     /gene="thiD"
                     /locus_tag="ACHALG_002920"
     CDS             complement(149..964)
                     /gene="thiD"
                     /locus_tag="ACHALG_002920"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFN2588213.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKDAQQETVNDYVLLESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(1166..>1762)
                     /locus_tag="ACHALG_002921"
     CDS             complement(1166..>1762)
                     /locus_tag="ACHALG_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFN2588214.1"
                     /translation="LHLPMQGKRKPNGYKEKRGKQAFKRNISERKKDYVVFEEEFGHL
                     EGDTIVGIHHKSAVITLVERLSKAIIALKPEGRKAVDIENSINEWLQSVPKNLFKSIT
                     FDCGKEFSNWKSISNTNDIDIYFADPGTPSQRGLNEHSNGLLRKDGLPKEMEFNQVNQ
                     GFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA"
BASE COUNT          460 a          371 c          299 g          632 t
ORIGIN      
        1 cttgtcacag tcccttcttc cgaacaaacg gtgttctcct gccaactcct cggtcacaag
       61 tcacaatatt caacaaacat gagaagctgt tttttgaact tgttccttgt gactcggagc
      121 tggacggcag gctcacgacc tctttctttt actctaattc accagctgcc caatgattga
      181 tgggtccata tttgtgccct acatttattt cttcacgaat tgcacggttt acatagtctt
      241 ttgccaatcg gatcgcttgt tcaactgttt ttcctttcgc taactcagct gcaatacatg
      301 cagatagcga atcgccagtt ccgttgatcc ggtcggtagg ataaaatggt gcatttaacc
      361 aaaacgattt tcctgattcg agcaatacgt agtcattgac tgtttcttgc tgcgcatctt
      421 tagcatgatg tcctttgatc ataacgtttt ttgcacccat atcttgcagt tcttttgctg
      481 cttgcacaaa atcttcttca gctgctaatg tccgtccagt cagcttttct gcttcataaa
      541 aattaggtgt cagtacttcg gccaaaggga caagcttttc tttcaagctt tgcaaagcag
      601 attcttctag taaaagattt ccatgtttcg ttacgattac gggatccacg actaatggtc
      661 caaaatcata tttttggtaa ttttttacaa ctgtctcaat caactttgaa tctgctagca
      721 tacctgtttt agctgcacgt atttgaaagt ccttcgcaat cagttcaaat tctttgtcga
      781 taaaatctgt tggcaatgtc acacttgctc cgattccgta agaattacct gcaacacatg
      841 cggtcatgac agaaacaccg taaacatttc ttgtaaagaa tgtatgcata tctgcttgca
      901 tccctgcgct tccatcgcta tctgatcctg cgatcgtcat tgcttgtaca tactgatctt
      961 ccatcaaatc gattcctcca tttttataaa aagatttttc ataatgctgt tactgtttct
     1021 atcatatgtt tttcaaagta tccctcagta aatataccac taccttttct tggtaatcaa
     1081 atttatttga aaaaacattt gtaataaatc agaaaatagt tgtcatttgt ttgtattcgc
     1141 tattctaata atattatttg tcaaattaag cgagacaaaa ctttccattt acgtaactca
     1201 aaaaaacttc taatggtgtt tggtaattta gtgattttct agggatatgg tttcttttgg
     1261 acgcaacgga tgagatgaat ccttgattga cttggttgaa ttccatttct tttggtagtc
     1321 catcttttcg gaggagtccg tttgaatgtt catttaatcc ccgttgggaa ggcgttcctg
     1381 ggtctgcgaa ataaatatca atatcattcg tattactaat acttttccaa ttagagaatt
     1441 cttttccaca atcaaaggta attgatttga aaagattttt gggtacggat tgaagccatt
     1501 cattaatcga attttcaata tcaactgcct tacggccttc tggtttcaag gcgataatgg
     1561 cttttgaaag tcgctcaacg agtgtgatga cggcactttt atggtggatg ccgacaatcg
     1621 tatcaccctc taaatgtcca aactcttctt cgaaaacgac ataatctttt ttacgttcag
     1681 aaatatttct tttgaatgcc tgttttccac gtttttcctt ataaccattt ggttttcgtt
     1741 ttccttgcat cggtaaatgt aa
//
LOCUS       DAZABD010000161         1756 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000161 DAZABD010000000
VERSION     DAZABD010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1756
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.171"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            133..750
                     /locus_tag="ACHALG_002922"
     CDS             133..750
                     /locus_tag="ACHALG_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFN2588215.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            791..1090
                     /locus_tag="ACHALG_002923"
     CDS             791..1090
                     /locus_tag="ACHALG_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HFN2588216.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            1124..1291
                     /locus_tag="ACHALG_002924"
     CDS             1124..1291
                     /locus_tag="ACHALG_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFN2588217.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(1336..>1756)
                     /locus_tag="ACHALG_002925"
     CDS             complement(1336..>1756)
                     /locus_tag="ACHALG_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFN2588218.1"
                     /translation="SLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGNELLD
                     TAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQKRLV
                     FKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT          574 a          289 c          346 g          547 t
ORIGIN      
        1 tatacttttt gacatgcctt gattttcttt gtttatgtat ggcataataa ccacataata
       61 caatttgaaa cgtttctgat ttaatctgat tgttttgaaa caattttagt gaaaaaaggg
      121 tagataaaaa cgatgagaaa atttttttat atccgggtca gtagttatga tcaatcgatc
      181 gaacgacaac tgatttccgc gaaggaatta ggaatccctg aagaatatat atttgtagaa
      241 aaagccagcg gcaaagattt taaacgccct gaatatcaat taatgaaacg gatgtttcga
      301 aaaggagatt tactctatct acaatcatta gatagactag gcagaaataa acaaatgatt
      361 ttagaagaat ggcgagaatt aactaaggaa aagcagattg atatttctgt gttagacatg
      421 cctctgttag atactgctag ttacaagcaa ctagatgggt tagagacgct tatctctgac
      481 ttaattctgc aattactcag ctatatggcc gaagacgagc ggaaacgaat taatagccgg
      541 caaagagaag gcgtagaagc tgcgaaacaa aagggtgtaa aattcggcag aaagaaaatt
      601 gctttacctc ctgaatttcc tgcgatctat tctgactgga aagcaggaaa gataactgca
      661 gtctatgcta tggatcaatt gggcatgaaa cgaaatacct tttatcgacg ggtgaaagaa
      721 tatgaacata ccttgcacca cttttcttga tagatataac agctattaga cgggaaggga
      781 gagtttgaaa atggacttaa cgaatcaaag ggtcgttttt actggaacgc tgcagcaaat
      841 gacacgaacc caagccatcg gtttggttca ctctttaaac ggccattgtc aatcaagtgt
      901 tacttcaaaa acaaatttcc tggtttgtgg tcagtattct aagtcgttgt ttgatgattc
      961 tctctataca aaaaaggaac aaaccgcgcg ggacttgatt gcgttaggtc atgagattac
     1021 cattttatca gaagtcgaat tttatgcgtt aattagtcaa cagttgaaag aatggcaacg
     1081 gtatttataa tcgttagtaa aatttacaag gaggaaaatt tttatgaagc gaaaatctta
     1141 tgatcgttgg ggcattgaaa taactagttt taatcgaaat attgcttttc acgtggagtg
     1201 tgactgtggg gaattagcaa aaagtgttta tcaaaaaaat tacttttact gtcctaaatg
     1261 ccatcgccga tacggggtac atggaacata gaaacgaaat agtgcggtag aaaagcgaat
     1321 gaatgtagta tatttctaca aatcattcgc tttctttatt ttataaatag ttggtcgaga
     1381 gatcccgttt tcttttgcaa tctctgcaac tggaatgcct tgctttaaca tgtccaccac
     1441 ggttttaaaa actaagcgtt tttgcggatc tgttgagtta atagaatact cgattggtcg
     1501 tcccttatat cggccggctt tttgagccaa taatactcct tgccgctgac gttcacgaat
     1561 tttctcacgc tcgttttgtg caatataact tagcagtgat aaaaacatat cgaacatagc
     1621 ggtatccagg agttcatttc cagtattaaa atctaaaaac tgtgcatcta atatttttaa
     1681 cgctactttt ttctgtttca taaaagcaac tgtatttttt atatcttcat aatctcttcc
     1741 taaacgatct agtgat
//
LOCUS       DAZABD010000162         1750 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000162 DAZABD010000000
VERSION     DAZABD010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1750
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.172"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            383..1633
                     /locus_tag="ACHALG_002926"
     CDS             383..1633
                     /locus_tag="ACHALG_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFN2588219.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     ETILEFNDSYHFDQIVIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFS
                     RMFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQH
                     FLENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGK
                     GRFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQ
                     VIVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQ
                     SQNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARK
                     FTRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          577 a          335 c          309 g          529 t
ORIGIN      
        1 ttgtctaata gtttttattc aaaatgtttc tagattataa aatttattga ataacacatt
       61 cctaacggaa tttgtttcga tattgtgtga tttctcagag tacaatggat gtatggaaag
      121 gatccaacgg gaatttgcta tttcctcctg tagcgtttgt ctaccctgtt tagtctgtta
      181 cccgaaccac tgttatcact accaactcat tagctgtttc gataatggac tagactttca
      241 aatagtagtt tggaaggcag cttctagctc ttttatcacc gtattctatt aggcgaggtt
      301 gtggctatac agtgagtttc tgggaaatcc cacagtatcg aagcattttc caaaatttat
      361 atcgaaagga ggtccttctt ccatgaaatt atttgtcggt attgacgtta gctctgaaaa
      421 actagatgtt tgttttttaa cagacgacaa tcaattgtct atcttatctg aaatctctgt
      481 tgccaatgac atcgaaggtg cttcattcat acgagaaaca attcttgaat tcaatgacag
      541 ctaccacttt gatcaaattg tgattggcat ggagtcaacg tccatgtaca gcttccatcc
      601 ttccatgttt tttcatgaag atgagaaact aaagaagctc aacacacttg taacgattga
      661 aaatcctttt cgggtgaaac aatttagtcg catgtttgat gagaataaaa ctgatcgaaa
      721 cgatgcatta agaatcgctg atttcttgcg tattcaacga ttcacaactt cccctatcaa
      781 agaagagaaa tacatggcct tacagcgttt aacacgtact cgctatcaac ttattaagca
      841 attgattcgt acaaagcaac attttcttga aaatctaacg tacaaatgta atacgcttgc
      901 tcgagaaatg cgagacgaat cgacgagtct ttttagtgca accctcattt cactcatgac
      961 ggaagatttc acattggatg aacttgctga gcttccttta gaagctttct gtgatttact
     1021 tcaagaaaaa ggaaaaggcc gtttcaagca accagaaaaa atcgctaaag caattcaacg
     1081 tgcaattacc atgagttacc gcctaggtgc tcttgcacaa gaatcaatta atgttgtttt
     1141 aagtgttctc gtacgagaaa tacgagcact tgaaaaaaat atcaaagaat tagataaagc
     1201 catcgaacaa gtaattgttg tcattccaga atatcaatgc ttaaccagta ttccaggcgt
     1261 tggtaaagtc tatgctgccg gtttaatcgc tgaaattggt caaatcgaaa gatttgaaga
     1321 tcagacaaaa ttggcaaaat acgctggatt aagttggaaa gtgaaccaat ctggaaacta
     1381 tcagtcgcaa aatacacctc tgacaaaaca aggaaatcga tattttagat actacttagt
     1441 tgaagctgcc aactctgtaa aaaattatct tcctgaatac aaagcttttt atcaaagtaa
     1501 atataaggag gttccaaagc atcaacacaa acgtgcactc gtcctaaccg caagaaaatt
     1561 tacgcgtctg gtggatacgc tactacgtaa acaccaactc tatacgccac caaggagtgt
     1621 gatagagaaa taacaatccg ttattgacgc aaatccttga atcaacccga aaaaaatttg
     1681 attttgatcg ggtctagttt cgtgcgcttc aaaacatatt actcaataaa gaaaatctta
     1741 aatttcatct
//
LOCUS       DAZABD010000163         1667 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000163 DAZABD010000000
VERSION     DAZABD010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.173"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            109..1328
                     /locus_tag="ACHALG_002927"
                     /pseudo
     CDS             109..1328
                     /locus_tag="ACHALG_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          551 a          305 c          317 g          494 t
ORIGIN      
        1 ttaaggggct ggcgagtggt gagcccttct agggctgtaa aaaaacatat cgttctctag
       61 tatgattaaa tcacgacaaa ataaacaact gagaggacga tatgctatat gaatgattct
      121 atcaaaaaaa tgctgagaat aacagagaaa gatttgatga ttacagaggt ctcttacgag
      181 acccttcaga agaaaaagac gttggtcgtc gatgctgttc tctcgcctgc tcctcgtgct
      241 tgtagaagtt gtggttctac tgtggtagat ggaaacggga aagcaattat agtgaaaaat
      301 ggaaaaaagg aaacgattgt ccgttttgaa caatacaatc atataccttt ggttatgcgc
      361 ctaaaaaagc agcgctatac ctgcaaaaac tgtcgaacct tttggactgc tcaaagttat
      421 tttgtccaac ctagacattc aatcgcagat catgttagat ataaaattgc tcctttactg
      481 actaaaaaag tatcttttat tgcgaaaagc tgtcaggtat ctttgaccac cgttattcgt
      541 acattaaaag agtttaaaag ctattgacca aagcaatcta agaagattct cccaagagta
      601 ttgatggttg atgaatttcg ttcgcatgct tccatagaag ataaaatgag ctttatttgc
      661 gcagatggcg aaacaggaaa attaatagat gttttgccca cgcgtaaatt acctcgatta
      721 acaagctatt tcttagggtg taccaatcca gaagaagtag aattcttggt gacagacatg
      781 aacgccgcct acttctagct caccaaacat gttctgccaa aagcgaaaat agtgattgat
      841 cggtttcata ttgtcaaaca caggcagaaa aactgaaaaa gaactggcgc tttttgttaa
      901 aaaatcgtgc aaacatcaac cattatgaat acaaaacatg gaaaagtttc cgagcaccaa
      961 aatacccatt tcttactgaa gcaatgatga ttgatcgatt gcttgagttt tctacgcccc
     1021 taaaggaggc gtatcccttt tttcatgaat tagttgaaga ctttcgagac aaagatccta
     1081 acttattttt ctccttattg acagaacttc ctgaaacgtt agatgacagc tttcgggaaa
     1141 agcttcaaaa ccttcttacc catgaataag gcatcaccaa tgcaatgatc tatccttaat
     1201 ctaatggaaa aatagaagcg aggaataacc acataaagac aatgaaacga atatcctacg
     1261 gatttaaatc attcgagaac atgagaatta gaatcttttt gatcaatcaa ttaatcaaag
     1321 tgagataaca aaaaatctga gccagaatga ggttcattcg gacccagatt tgattttgca
     1381 caactcatca gtccttattg acaaagagcc aaaaaatgat catcgggctt ttttacgctt
     1441 ataatcttag ttcttgtaga atttcaggtg ctttatcagt ggtcccgttt tcacaggtga
     1501 ttacttaccc aagagaataa gggaaaactg tatccagttt tcaaatggga ccttgcagcg
     1561 caagaagggt gtggggcttt ttaatgcaaa gggattttgc aacttttgcc catgttatat
     1621 ttgatttcta tcgctttttt cttttttgtc tgtttatttc taacttc
//
LOCUS       DAZABD010000164         1654 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000164 DAZABD010000000
VERSION     DAZABD010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.174"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(342..1241)
                     /locus_tag="ACHALG_002928"
     CDS             complement(342..1241)
                     /locus_tag="ACHALG_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002889672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFN2588220.1"
                     /translation="MTIELKNEYLTVQFKTLGGQLTSIKDKDGIEYLWQADPNYWNGQ
                     APILFPICGSLRNDWAIYRPQERPFFTGLIRRHGFVRKEEFILEEVNDNSVTFSIKPN
                     AEMLDNYLYQFELRVVYTLNGKSIRTEFQVTNLEMEKTMPYFIGAHPAFNSPLVEGEN
                     YEDYYIEFSEVESCSIPKSFPETGLLDLQDRTPFLENQKILDLDYSLFSHDAITLDQL
                     KSRSVTLRSRKSGKGLRVDFDDFPNLILWSTTNKSPFIALEPWSGLSTSLEEGNFLED
                     KRQVTKISPQETSRKIYDITILN"
     gene            complement(1262..1534)
                     /locus_tag="ACHALG_002929"
     CDS             complement(1262..1534)
                     /locus_tag="ACHALG_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HFN2588221.1"
                     /translation="MDTVRDFILYLKLCNRTEKVYIVSFEDCPMIPELEATPLLKCGK
                     WYVSTGKEWICHSDLELSAFEWEFLQSLDVEIRETIHFEVNYLPFQ"
     gene            complement(1538..>1654)
                     /locus_tag="ACHALG_002930"
     CDS             complement(1538..>1654)
                     /locus_tag="ACHALG_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFN2588222.1"
                     /translation="TIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLLGIPA"
BASE COUNT          552 a          316 c          254 g          532 t
ORIGIN      
        1 acgctatatt taagatttca aacaactata ttaataggaa gaaaatttca tttttcctct
       61 tagttctata aaaagtgtgg ctaatttgag gcaaaatatt aaattaaatt tgccacaaat
      121 taatggctcc actattattt taaatcaaga agtagtaaat aatcttattt tttactacca
      181 gactgtcttt agatattagc ttttcgtcac ccactacagt tgacatagac ccacaaaaaa
      241 cttaaaaacc ttgcgatgta agactggcac ggagaagacc aatctatcat cacaaggtaa
      301 gagtattcaa atcttattga agactcaaat aataacaaat tttaattaag gatagtaata
      361 tcatagattt ttctagaagt ttcttgaggt gaaattttcg ttacctgtct tttatcttct
      421 aagaaattac cttcttcaag tgaagtggag agaccactcc aaggttccaa agcaatgaat
      481 ggacttttat tagttgtaga ccacagaatc aagttcggaa aatcatcaaa atctactcga
      541 agacctttac cagacttcct tgaccgaagc gttacgctac gagacttgag ctggtcaagt
      601 gtaatggcat catgggaaaa aagtgagtag tccaaatcga gaattttttg attttccaaa
      661 aaaggtgtcc gatcttgcaa atctaataat cctgtctcag gaaaactctt tggtatcgag
      721 caagactcaa cctcactaaa ttcaatataa taatcctcat agttttcacc ttctactaaa
      781 ggactattga atgctggatg ggctccaata aagtaaggca ttgttttttc catttccaag
      841 ttcgttacct gaaattcggt tctaattgat ttaccattca aggtataaac aactctcagt
      901 tcaaactgat ataggtaatt atcaagcatc tcagcatttg gtttaatact aaaggtcacg
      961 ctattatcat tgacttcttc cagaataaat tcttcttttc gaacaaaacc atgtctacga
     1021 ataagacctg taaaaaaagg tctttcttga ggtctataaa tagcccaatc atttcgtaaa
     1081 ctaccacaga taggaaatag aataggcgcc tgtccattcc aataatttgg atcagcttgc
     1141 cagagatact ctatcccatc cttgtcttta atcgaagtca gttgaccacc aagtgtttta
     1201 aactgaacag taagatactc gttttttaat tcgatagtca caactaattc ctttcttttt
     1261 attattggaa aggcaaataa tttacctcga agtgaatagt ctcacgtatt tctacatcga
     1321 gtgattgtaa aaattcccat tcaaatgctg ataactctaa atcagaatgg caaatccact
     1381 cttttccagt tgaaacatac catttaccac attttagcaa cggggtagct tcaagctcag
     1441 gaatcatagg acaatcttcg aaactaacaa tatatacctt ttcggttctg ttgcaaagtt
     1501 ttaaataaag aataaaatcc cttacggtat ctatgattta agctgggatt cccaataata
     1561 ccttgatttc agtacagacc gaaaacccga agagagtgcc ttcttttcgg gttttcttat
     1621 ataatcctcg gatggcttcc atgcctttaa tcgt
//
LOCUS       DAZABD010000165         1629 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000165 DAZABD010000000
VERSION     DAZABD010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.175"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..355)
                     /locus_tag="ACHALG_002931"
                     /pseudo
     CDS             complement(1..355)
                     /locus_tag="ACHALG_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            583..1125
                     /locus_tag="ACHALG_002932"
     CDS             583..1125
                     /locus_tag="ACHALG_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2588223.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            1281..>1629
                     /locus_tag="ACHALG_002933"
     CDS             1281..>1629
                     /locus_tag="ACHALG_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HFN2588224.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMP"
BASE COUNT          587 a          217 c          309 g          516 t
ORIGIN      
        1 tgacgagaaa tatttagaat tttgcacatc gctgatatgg aatatttatg cttgttggca
       61 tcaattactt gtcttttcgt ccgaatatca gcgccgcttg ctttaaaata tcattctcca
      121 tttcgagttg cttatttttc tttcttagtg cgatcaattc tgcttgttct ggtgttaagt
      181 tgtctctttc tttgaatgag cccgttgatt gtgcttgctt catccattta tcgaaagaag
      241 aaggcgtaag ctcatactct cgaataattc ctgcgcaagg cttaccagcg agatagagat
      301 cgacgatttg ttgtttgaat tcttttgaat aggttcttcg ttgacgtctt ggcatgaaaa
      361 atcctccagt gtgtttttta ttattctaca caccttattt tttctgtcta gtctagtgta
      421 gccgattcaa gttcttcgtg ttctcttaga cataaaaatt cctcctggta tgttttcttc
      481 tagtctacac accttaattt ttctgtctag ttaattgtag cctatccagt ttggaaaaat
      541 aaaaaattat atttcatcaa cgtttcataa aaggtgtgta gaatgttatt gaaagtagct
      601 ataaaatgct attgtgtaaa aaataaagga ggaataattt atgaaaaaat agtaccaaga
      661 ggagcatgcc tattacttgc attatgtttg agcttaagcg cagttgggtg tcaaaaaagt
      721 aatgaaaaca ataaaataaa agaaggacaa tccataagtt ctgaagagga aggcatgtca
      781 attaatgcaa atggcaaaaa tgaaactttt aagccatcag attatacatt ggaggcaaag
      841 aaagaatatg tttatgaata tttagggctt aaatttaaac tctcagacaa atttagaaat
      901 tatatagctg ataaaaagat agcgatgtta gatgatcaaa gtcctataga taaagaacta
      961 aagtatgcta ttttgacatt tgagaaaatg acagaagaac aaaagaatgc agttatagaa
     1021 aagatgggcg atggatataa aaattggcaa aatgagcttg aaagaattgg gactattggt
     1081 atatttgaaa aaaatacatc agaagaaaaa aatctaaaat tataaaatgt gatactcata
     1141 ctaaaatagg agtttcaagt gatggaaaat acgattgcta tttaagcaca aatagcggag
     1201 ctgaaagtaa tcttcttgat gaattaaaaa gaactgagat tcaaattata gataaaaaag
     1261 aacgtcctaa aaatggtttt gtgctatcag aaaaaactga tttagaaaat acagaagcat
     1321 ttaacaaaga atcggttaaa gatttacgta aactaccaac aaaggacata aatggcaagg
     1381 attttacaag taaagatttt gagaagtatg atcttactat ggtaaatgta tttgcaactt
     1441 ggtgtacagc ttgtgtaaag gaaattcctg atttggttga ggttcagaat gaaatgaaaa
     1501 gcaagggtgt aaatatcgtt ggagttgtta cagatcctgt tgatgataac ggtgaaaaca
     1561 aggaagcaat tgaaaagtca aagttaatac atgaaaagac aaaggcttca tatcctttct
     1621 taatgccag
//
LOCUS       DAZABD010000166         1552 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000166 DAZABD010000000
VERSION     DAZABD010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.176"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            175..579
                     /locus_tag="ACHALG_002934"
     CDS             175..579
                     /locus_tag="ACHALG_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2588225.1"
                     /translation="MIILCFENKGYFFEISDIAGFVEVERKKQCSSATLNTYDYVLIN
                     IVKQLSEQQKKLIYLINSKNFNTTLSNLIEMDAKISDYLFFLINCRFENDKEVIECVE
                     SVEKDGLRDYYCGLNFSDNINNYKLIVHTTKY"
     gene            1143..1379
                     /locus_tag="ACHALG_002935"
     CDS             1143..1379
                     /locus_tag="ACHALG_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2588226.1"
                     /translation="MDKGVADIAKIKQVLKQESIKSLVEGTGLSKSTISSLKSGTRKV
                     EKLNLFAAIKLTEYSDQVFKPIIEIWGKELKKQL"
BASE COUNT          602 a          161 c          243 g          546 t
ORIGIN      
        1 aaaaacgaac actcatgttg gcgatttaga tgatataatt tatgtgacac aaagaaaact
       61 tagaaatatg tttcaagaga aataattact gtttgtctaa tttcatcatg tgtatgaaag
      121 aaaccaataa atttgtcttt tttcaaagaa aacttatttt aataatttga gaggatgatt
      181 atattgtgtt ttgaaaataa agggtacttt tttgaaatct cagacattgc tggatttgtt
      241 gaagttgaaa gaaagaaaca gtgtagctca gctacattaa atacttatga ctatgtttta
      301 attaatattg taaaacaatt gagtgagcag caaaaaaaat tgatttactt aataaactca
      361 aagaatttca atactacact aagcaattta attgaaatgg atgccaaaat ttctgattat
      421 ctttttttct tgattaattg tagatttgaa aatgataaag aagtaataga gtgtgtagaa
      481 agtgtagaaa aagatggtct tagagactac tattgtgggt taaatttcag tgataacata
      541 aataactata aattgatagt tcacacaact aaatattaat ttttttagaa cgatgttaaa
      601 agatggatga tataggggat tagttatttt aactagtatt tatttaggag aaattttaga
      661 aagaaaagat cgattattta tcctgagtta gaagtagtaa cctttcggtt taattttaat
      721 gataaagtga tatttactca aaatttaaaa acaaataatg aaaatgaaaa gctttatgaa
      781 ccaacaaata ttgataccca agatgaaaaa actaataata caacaaaagt ttatgacggt
      841 tggtattatt taggctcttt gtcaaatagg actgatagag tgatataaga acatcggctc
      901 tataattatg agtattatgc tggattagac gtccatcaaa ataatttttt gttttgaaag
      961 gttcctttat tcagataaat aggatgttgg agtatatcta tgttgcttat gtaatacaat
     1021 ttatttttat tgtatatttg ttcccttttc gtacttttaa acttttacta ccagagcata
     1081 ctaaaatatg ctatattttt actacagtag ttataaattt gagataaaaa aggagtcttt
     1141 ttttggataa aggagtcgct gatattgcaa aaattaagca agtgttgaaa caagaatcta
     1201 taaaaagctt agtagaagga actggtttaa gtaagagtac tatttcaagt ttaaaatcag
     1261 gtactcgaaa agtagaaaaa ttaaaccttt ttgcagctat taaactaacc gaatattcag
     1321 atcaagtttt taaacctatt attgaaatct ggggaaaaga actaaaaaaa caactatagt
     1381 tatcgaataa aactaataga tttaaaaata gaagaactag atatttttca gatgaatgct
     1441 tgtttagctc tacaatgggt agaactgaaa gataatattt ctcagttcta ccaattataa
     1501 agttataatt ttattcaatc tcaatgagtc ttttttttac caatattttc ag
//
LOCUS       DAZABD010000167         1548 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000167 DAZABD010000000
VERSION     DAZABD010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1548
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.177"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(633..773)
                     /locus_tag="ACHALG_002936"
     CDS             complement(633..773)
                     /locus_tag="ACHALG_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002381035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HFN2588227.1"
                     /translation="MYVSIKSRERRRLLSVSEALGLMINFGTLIIALIALVVTLIKQN
                     KK"
     gene            1046..1498
                     /gene="tnpA"
                     /locus_tag="ACHALG_002937"
                     /pseudo
     CDS             1046..1498
                     /gene="tnpA"
                     /locus_tag="ACHALG_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334252.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT          524 a          253 c          291 g          480 t
ORIGIN      
        1 aatcatttgt ttaaaatttt cttcatatcg ggtcataaaa attcatcctc tcgtagataa
       61 gtttaattta ttcttgtctc tacgaaaagc tgtctaagat ttgatactaa caccagttgc
      121 aacaggaaag ggtagattaa atatagatga ttaaaaattt gaaatatata tctataggta
      181 caattatcat caatattatt ctcaaccttt tatttttcac taggacaact attgatgtca
      241 ccatactatt atttagtttt cctataggaa ttgtgggaat tatagctagt tgttatctag
      301 aaaagaaaca tatgagggta gtttttgttt tagttaattt cttattggct ataagctttt
      361 tgtatccgtt tttattatgg aaattagcaa gtaagccgtg agagaataga cgaaaaaatt
      421 ttgttgcccg ccacactcca accgctacca ccgtttaggc gggcaacaaa aaagctggca
      481 aagccagctt tgcagttcca tcgaacatga aagtatgcta taataaatac gaaaagagga
      541 actgtccggc agtccctctt tggtagcacc gttcaagacg gtggccaagt taactattta
      601 taaaaaatag ccgtactccg ctaaagtgac ggctattttt tgttttgttt gattagcgtt
      661 accactaggg cgattaatgc gatgatcaat gttccgaaat tgatcattag ccctaacgct
      721 tctgaaacag acaaaaggcg tctcctttct ctagatttga tacttacata cataagcacc
      781 acctcctgaa ataggattgc caccgtcata aacctttact accaaatcta ttataccata
      841 aaaaaaccgt tagaaacgtt gatttttcaa tgtttctagc ggcttttttt gtttattgac
      901 tttagtcagt ttcgttcgcg tagcgtacga aacaaaaaaa ccgcgcgttt ctacgcgtgg
      961 acgaaatagc tttagcgata aaaaactcct ttcgatatac taatagtggc taccaaccgc
     1021 atatcagatc gaaaggagtt tttttatgtt taactacgat aaaagtttag cacataccag
     1081 atggaattgt aaggttcaca tagtcttcac cccgaagtat caacgaaaag tgatttatgg
     1141 aaagttaaga gtaagtattg gtcagatagt aagaaaattg tgtgagatga aagatattga
     1201 aattgttgaa gcgcatgcaa tgcctgacca tattcaatta ttagtaggaa taccgccaaa
     1261 gattcgtatt tttagtttta tgggctactt aaaaggacga agtgcaacct taatccatga
     1321 aaaacatgcg aatttgaaat ataagtatgg gaataaaagc ttttggtcca aaggatacta
     1381 cgtgagtacg gttggggtaa atcaaaaaac aattgaaaaa tatatttgag agcaagaagc
     1441 agatgatcga ataagagata gtataagtaa acaagagtat atagatccat ttaaatagtt
     1501 aaagagagat agggctgtga gcggttggtg gtcatttaaa aagcccct
//
LOCUS       DAZABD010000168         1531 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000168 DAZABD010000000
VERSION     DAZABD010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.178"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1342
                     /locus_tag="ACHALG_002938"
     rRNA            <1..1342
                     /locus_tag="ACHALG_002938"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            1437..>1531
                     /gene="rrf"
                     /locus_tag="ACHALG_002939"
     rRNA            1437..>1531
                     /gene="rrf"
                     /locus_tag="ACHALG_002939"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          428 a          329 c          449 g          325 t
ORIGIN      
        1 gaaataatag taccgaagtt cctgatgtca cactgccgag aaaagcttct agtgagaaaa
       61 cagcggcccg taccgcaaac cgacacaggt agtcgaggag agaatcctaa ggtgagcgag
      121 agaactctcg ttaaggaact cggcaaaatg accccgtaac ttcgggagaa ggggtgctga
      181 tcatacgatc agccgcagtg aataggccca agcgactgtt tatcaaaaac acaggtctct
      241 gcaaaatcgt aagatgaagt ataggggctg acgcctgccc ggtgctggaa ggttaagagg
      301 agtgcttagc gcaagcgaag gtacgaattg aagccccagt aaacggcggc cgtaactata
      361 acggtcctaa ggtagcgaaa ttccttgtcg ggtaagttcc gacccgcacg aaaggcgtaa
      421 cgatttgggc actgtctcaa cgagagactc ggtgaaattt tagtacctgt gaagatgcag
      481 gttacccgcg acaggacgga aagaccccat ggagctttac tgtagtttga tattgagtgt
      541 ctgtaccgca tgtacaggat aggtaggagc cgtagaaatc ggaacgctag tttcgatgga
      601 ggcgctggtg ggatactacc cctgcgttat ggccactcta acccgcacca ctaatcgtgg
      661 tgggagacag tgtcagatgg gcagtttgac tggggcggtc gcctcctaaa aggtaacgga
      721 ggcgcccaaa ggttccctca gaatggttgg aaatcattcg aagagtgtaa aggcagaagg
      781 gagcttgact gcgagaccaa caagtcgagc agggacgaaa gtcgggctta gtgatccggt
      841 ggttccgcat ggaagggcca tcgctcaacg gataaaagct accctgggga taacaggctt
      901 atctccccca agagtccaca tcgacgggga ggtttggcac ctcgatgtcg gctcgtcgca
      961 tcctggggct gtagtcggtc ccaagggttg ggctgttcgc ccattaaagc ggcacgcgag
     1021 ctgggttcag aacgtcgtga gacagttcgg tccctatccg tcgcgggcgt tggaaatttg
     1081 agaggagctg tccttagtac gagaggaccg ggatggactt accgctggtg taccagttgt
     1141 tctgccaagg gcattgctgg gtagctatgt agggaaggga taaacgctga aagcatctaa
     1201 gtgtgaagcc cacctcaaga tgagatttcc catttcttta agaaagtaag atccctgaga
     1261 gatgatcagg tagataggtc aggagtggaa gtacagtgat gtatggagcg gactgatact
     1321 aatcgatcga ggacttaacc aataggttaa aagaaaagaa ctcggaaaaa aacttatgtg
     1381 acttcaaatc cagttttgag tgaacaaaat ttactcaata aaagataaca ccacagtgtg
     1441 gtggcgatag cgagaaggat acacctgtaa ccatgccgaa cacagaagtt aagcttctta
     1501 gcgccgattg tagtgagggg ttgccccttg t
//
LOCUS       DAZABD010000169         1462 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000169 DAZABD010000000
VERSION     DAZABD010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.180"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1359
                     /locus_tag="ACHALG_002940"
     CDS             <1..1359
                     /locus_tag="ACHALG_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFN2588228.1"
                     /translation="LFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLPITEKE
                     HLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEKEGLSS
                     FKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRDNITNW
                     HIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKDSQHPI
                     VYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFFEAIPK
                     GKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWMATIDP
                     VAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHSTSLEG
                     ARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDVQQHFS
                     RN"
BASE COUNT          482 a          266 c          308 g          406 t
ORIGIN      
        1 ctttttagtg atgaagaaat ttctgtaagt gttccagaag cagctgccac cattattcca
       61 gtaaaaggac atcaacgagt tgtaggaacg aaagcagaca aaatcaaaca cttacctata
      121 acagagaaag aacatctcct tcctttggaa gaacaatttt gtgaacattg cggttcacag
      181 atgaaagata tcggacggac aaaagtacga gaagaaattc gtttccatca ggctatgttg
      241 gattgtctca cccattatca acatacctat tgttgtaaaa gctgcgaaaa agaaggactt
      301 tcttctttta aaaaagcgat cgttcctaaa cctttgattt ctaatagctt aggttccaat
      361 agtttagtgg cggaaacgat ccggatgaaa tttggtcaga aagttcctgc ctatcggcaa
      421 gagaactatt ggaaacaaac acatgggttg gatatttcga gagataatat caccaattgg
      481 catattaaag cagtgcaaaa cgcgttagac cctttaggag aacgattaag agtctatctt
      541 aatcaagaag agattctaca cggggatgaa acaagttatc gtgtgattga aagtgccaaa
      601 acagatacct attactggca gttttgtaca ggaaaagaca gtcagcatcc catcgtttat
      661 tatcaccacg acgaaagtcg tgctggagat gttccgaaaa cttttctcaa ggagtttaca
      721 gggtatttac actgtgacgg ctatagcggg tataatgccg tggaaagtgt acgacttgtc
      781 tattgtttcg cgcatgttcg gcggaaattt ttcgaagcaa ttccaaaagg aaagaaaaac
      841 acggatattc ccgcagctca agcagtcaaa caattggata aatggtttgt cctagaaaag
      901 aaatggaaag acttttctcc tgagaagcga ttaagctgtc ggcaacaaga actgcgtcca
      961 ttatttattg ctttttatga gtggatggca acgatagatc ctgttgcaaa atccaagttg
     1021 gatgcagccg ttcaatatgc gtgtaaacta cgaagtggtt ttgaacctat ttttgaagat
     1081 ggtcgtttag aactcacaaa taatcgtgcg gagcgaaata tcaaagagct ggtgatagga
     1141 cggaaaaatt ggttgcattc cacaagtctt gaaggagcac gaacttcagg aatcattctg
     1201 agtgtatata aaacagcaga acttaacgga ttgaatcctg taaagtatct agagtttcta
     1261 tttgataaga taccaaatct tcccgtactt tcagcagaaa ctttagatca actgttgcct
     1321 tggaacaagg atgtacaaca acatttttct cgtaactaaa aacgccaaac attcccgatt
     1381 ctattctata ggtcgggtta tttgacgtac aggtactgtt ctatggggcg cttacttttc
     1441 ttcttcaaga ccaaataaaa aa
//
LOCUS       DAZABD010000170         1455 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000170 DAZABD010000000
VERSION     DAZABD010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.181"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1040
                     /locus_tag="ACHALG_002941"
     CDS             <1..1040
                     /locus_tag="ACHALG_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HFN2588229.1"
                     /translation="EINQKYDETLKNVYSSLIVSNIEETSSSEIKNKLSLLKLKEKEY
                     IIDDAVSVPLILNKREKNNLKKKMLIPFDSEVKGLLNKLTISNIDSTREKIIKIFDKI
                     NNLFKNDNAELRKGLLEFKLQELELHYSYLVKITEEKEQQKAIKEQMIEEEKVRREID
                     REKKRIDKEQRQFNSEISKLIAYMQKANADVEKELYANKIQELEEKLKELEVIRENVL
                     QRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEPMDRIKELSSASVPFEFDVHAMIF
                     SEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLDEIEKVVLENYNGTVTFTKLAKAE
                     QYRRSLELSKD"
     gene            1112..>1455
                     /locus_tag="ACHALG_002942"
     CDS             1112..>1455
                     /locus_tag="ACHALG_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2588230.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQ"
BASE COUNT          640 a          169 c          227 g          419 t
ORIGIN      
        1 atgaaataaa tcaaaagtat gatgaaacat taaaaaatgt ctattcttca cttatcgttt
       61 caaatataga agaaacttca tcttctgaaa taaaaaataa gctttctttg ttaaaattaa
      121 aagaaaagga atatattatt gacgatgctg tttctgtccc tcttatttta aataaacgag
      181 agaaaaataa tttaaagaaa aaaatgctca ttccatttga ttcagaagtg aaaggattac
      241 tcaataaact aactatttct aatatagact cgactaggga aaaaatcata aaaatatttg
      301 ataaaattaa taatttattc aagaatgaca atgctgaatt aaggaagggt cttttagagt
      361 ttaaacttca agaactagaa cttcattact cttatttagt gaaaattacc gaagaaaaag
      421 aacaacaaaa agcaataaaa gaacagatga tagaagagga aaaagttcgt agagaaattg
      481 atcgtgaaaa gaaacgtatt gataaagaac aacgtcaatt caattctgaa atttccaaat
      541 taatcgcata tatgcaaaaa gctaatgcag atgtggaaaa agaattatat gcaaataaaa
      601 ttcaagaact ggaagaaaaa ttaaaagaat tagaagttat acgtgaaaat gtattacaaa
      661 gagaattaaa tactagagcc ggttatgtgt atgtaattag taatattggg tcttttggtg
      721 aagacatata taaaattggt atgactagaa gattagaacc tatggataga ataaaagaat
      781 tgagtagtgc atcggttcca tttgaatttg acgttcatgc gatgattttt tcagaggacg
      841 ctccgtcttt ggaaactaaa ttgcataatc attttagaaa acaagaagtt aataaaatta
      901 atcaaagaaa agaatttttc aaagtttcac tagacgaaat agagaaagtt gttttagaaa
      961 attataatgg aacagtaaca tttacaaaat tagctaaagc tgaacaatat agacgatctt
     1021 tagaattgtc aaaagattaa atatataaaa agccttcggg ctttttcttt taacaattaa
     1081 taggaacgta tattcgaaag gaatagaaat aatgtggtta gaaaaaataa atgacaaaaa
     1141 atttaagtac accgaacgtt atacggatcc cttaactgaa aaaaagagaa aagtgtccgt
     1201 tactttatct agcaattcac gccaagcttg gaatcaagct aacttacttt tgaatgaaaa
     1261 aatcgcagaa aaaataaaac gaaatgaaga attaccacta acttttggag aattaaaaac
     1321 taaatgggat gaaaaataca agccaactgt aaaagaaagt tcctatcgaa caactcaggt
     1381 ttatctatca ttaatttcaa aatatataaa agatgatgta cttgtaaaaa acgttaatag
     1441 taatcttata caaga
//
LOCUS       DAZABD010000171         1440 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000171 DAZABD010000000
VERSION     DAZABD010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.182"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            41..451
                     /locus_tag="ACHALG_002943"
     CDS             41..451
                     /locus_tag="ACHALG_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFN2588231.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            465..821
                     /locus_tag="ACHALG_002944"
     CDS             465..821
                     /locus_tag="ACHALG_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFN2588232.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          526 a          215 c          273 g          426 t
ORIGIN      
        1 ttactgtaga attggattat aattaaaaag gagtgagaaa atgataaaag tatatacttc
       61 gcctagctgt acttcctgta gaaaagcaag agcatggttg caagcgaatc aacttgaatt
      121 tgaagagaag aatatttttg ccgaaccgtt aacagaaagt gagatcaaaa agatcttggc
      181 agcgactgaa ggaggagttc aagatattat ttccactcgg tcgaaaattt acgaaaagct
      241 gggcatagat tttaacgaat taactttaaa gcaaatgatc gtcttattca aagaatatcc
      301 ttcattattg agacgaccta ttttgattga tgaaaaaaga atactaatcg gatttaatga
      361 agatgagatc cggtcatttg tccctcgtga aattcgatgt gttgccagaa atctgatcta
      421 tttacaaaac aataacatct taaattcata gggagggagc cttcgtggaa gtgaaagtct
      481 atggatcagc gcactgtgta acaagtctga aggcgaaaca atggttagaa caccatcaat
      541 tgccttatca atttgttgac ttagaagaac gcgatctaag tacagaagaa gcagaagaat
      601 tatgcagttt tgaagaagtg caagcagaac agctatttgc gacttggtct gaggcttttc
      661 acaacattgc gattgacctt tcctgtgtag gaaaaaaaca actgattttt ttatgcacac
      721 aacataccaa tctcctcaga cgtcctttga ttattatcga tgaagttcta tttattggat
      781 acaatgagca gttgatggaa cagcttatct taaataaata gactttaact gttttaatca
      841 agtcactgag catttcatac agttgtaaca gtcgaacact tagcatcaag cgtgttatgt
      901 gttctttttt tgactttatt tttctactat agagacatgc tcaatttata gagacatgct
      961 caatttatgt ggtcaaaatt caaatctagg tggggatata gagttaaaaa tatattgaca
     1021 tgtgtaaatt tacatgatat attcatttct gttgttttaa gtagcaaagc aataaaatag
     1081 tattttaaaa aacatataaa atagttgttg acatgttgga aatgacatga taagataact
     1141 gagtcgttga aaaagaacca cttgcgaagc ttgattctat cgaaaaaaac ttcaaaaaag
     1201 ttcttgacaa ataacaaacg ataagttatg ataaatgagt tgctgaaaga tttcgaaaaa
     1261 cgaaatcgaa aaaaactttt aaaaaagagt tgacaatcgc ttgtctctct gatatgatat
     1321 aaaagttgct acggcagcca aaaacgaatc gggaaacact ctcgatgaag tagacctttg
     1381 aaaactgaac aaagtaagac aaaccaaatg tgtagggcgt cttgattcaa ttcaagacaa
//
LOCUS       DAZABD010000172         1424 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000172 DAZABD010000000
VERSION     DAZABD010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.183"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(150..1337)
                     /locus_tag="ACHALG_002945"
     CDS             complement(150..1337)
                     /locus_tag="ACHALG_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HFN2588233.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          414 a          309 c          249 g          452 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttcact ttta
//
LOCUS       DAZABD010000173         1409 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000173 DAZABD010000000
VERSION     DAZABD010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1409
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.185"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..1163)
                     /locus_tag="ACHALG_002946"
                     /pseudo
     CDS             complement(1..1163)
                     /locus_tag="ACHALG_002946"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
BASE COUNT          435 a          311 c          176 g          487 t
ORIGIN      
        1 atcagtagat agatcaggat ctagtgacaa cgctaatgca gcactaccag tgacatgagg
       61 tgttgccatc gaggtacctg acatatacgt gtatttactt cctagaaatg tactaaaaac
      121 acgaaaccct ctagcgaata aatcaacacc tgttttacta taatttgaat cagaacttcg
      181 ttcataacca ctagaatttc ctacagtaat aatattaggt atatccaaag aagctgggta
      241 aaaaggttta tcagtcatat ctatcccatt atttccagct gctgctacga ataatccagg
      301 atagttttta attgcttctt ctgtactgct ataaacaaca ctacctatac taaaattgat
      361 aattggaaac cctttttggg atgcataatt aatagcttca gttaagattg ccaaactggt
      421 acctccacta ctaccaaata cacgtatagg tactatcgta atatcccaat ttattccagc
      481 aacacctaat tcgttgtttg tttgagcacc aataatcccg gcaatatgtg tccatgtccc
      541 atagtatcca taggatctgt atcatcgtca aaaaaatcgt atctcaaact ttcatctaca
      601 tttccagaaa gatcttcatg ttgataatca attcctgaat caatgaccgc taccttcaca
      661 tccttagaac ccttggtttc ttcccaagcg gatggcatgc taatctctct taaattagct
      721 tgtttactcc aattaggatc gttaagaata attgatcgat ttcctaaaga atcagttgta
      781 tttggctcct caacatcatt caacggctct agttcaacca catagtttgg ctctgcataa
      841 ttgaccatat cagaagcatt aaatgttctt actgcctctt ccacactaat ttcttcgttc
      901 aatttcacta ataatacttg atctcgctta ttataatcgc tttcatttgt cctaagagtc
      961 aaatcttttt ccaccataac ttttacaccc aataaatttt gcggtttatt tttctctgtt
     1021 gaaattcttt gtgtagcctt taaactgaca attagctcat tgggtacata ctctatttga
     1081 gagtcatcta attggctcat aatcctttct ttttggttag tatctgcagt tacttgttta
     1141 gtatgagcca tgaacccaaa catactcacc ccaacaacca atcctaaata tattttcttt
     1201 ttaaactttt tcacctacat ctcctcctaa tagttttatt ttagagttag ctctattact
     1261 tatttactaa ctcaacaaca gattatcata cacttgttaa ttaaatcaca atatctcatt
     1321 aaaacacacc ctaaaatcat aaccgttcct atgctcattt tttggatttt ttaatcacgt
     1381 aaccagaaaa ataaatcccc tctatttca
//
LOCUS       DAZABD010000174         1389 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000174 DAZABD010000000
VERSION     DAZABD010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1389
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.186"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..170
                     /locus_tag="ACHALG_002947"
     CDS             <1..170
                     /locus_tag="ACHALG_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFN2588234.1"
                     /translation="YHRTLTTYLNTLLSNSFIINQIVEPQPPENMMDIPGMADEMRRP
                     MMLIVSAKKKM"
     gene            203..1111
                     /locus_tag="ACHALG_002948"
     CDS             203..1111
                     /locus_tag="ACHALG_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HFN2588235.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            1108..1335
                     /locus_tag="ACHALG_002949"
                     /pseudo
     CDS             1108..1335
                     /locus_tag="ACHALG_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          496 a          200 c          305 g          388 t
ORIGIN      
        1 aatatcatag aacactgacc acatatctaa atacactgct ttcaaatagt tttataataa
       61 atcagattgt ggagccacag ccgccagaga acatgatgga tattccgggg atggcggatg
      121 aaatgcgacg cccaatgatg ctgattgtat cggcaaaaaa gaagatgtaa taatatagaa
      181 aaaataaacg aggagtatgt aaatgagatc agaaaaagaa atgatggatt tagtactttc
      241 tttagcagaa caggatgaac gtattcgaat tgtgaccctt gaggggtcac gcgcaaatat
      301 taatatacct aaagatgaat ttcaggatta tgatattaca tattttgtaa gtgatataga
      361 accgtttata tctaatgatg actggcttaa tcaatttggg aatataataa tgatgcaaaa
      421 gccggaggat atggaattat tcccacctga agaaaaggga ttttcctatc ttatgctatt
      481 tgatgattac aataaaattg atcttacctt attgcccttg gaagagttag ataattacct
      541 aaagggcgat aaattaataa aggttctaat tgataaagat tgtagaatta aaagggacat
      601 agttccgact gatatagatt atcatgtaag aaagccaagc gcaagggagt atgatgattg
      661 ctgcaatgaa ttttggaatg taacacctta tgttattaaa ggattgtgcc gtaaggaaat
      721 tttatttgct attgatcatt ttaatcagat tgttcgccat gagctgctga gaatgatatc
      781 atggaaggtc ggcatcgaaa caggctttaa attaagtgta ggcaagaact ataagtttat
      841 tgaaaggtat atatccgagg atttgtggga gaaacttttg tccacctacc ggatggattc
      901 ctatgaaaac atatgggaag cattatttct atgccatcaa ttgttcaggg cggtatccgg
      961 tgaggtggcg gaaaggcttc attatgccta tccggagtat gataggaata taacaaaata
     1021 taccagggac atgtataaaa aatacactgg taaaaccggc tgcctggata gcacatatgc
     1081 cgctgatata gaagagaggc gggaacagtg attacagaaa tgaaagcagg gcacctgaaa
     1141 gatatcgata aacccagcga accatttgag gtgataggta agattatacc gaggtatgaa
     1201 aacgagaatt ggacctttac agaattactc tatgaagcgc catatttaaa aagctaccaa
     1261 gacgaagagg atgaagagga tgaggaggca gattgccttg aatatattga caatactgat
     1321 aagataatat atcttttaac ccgtggtgct agttaatttt aatatcctaa agaatatctc
     1381 aaagttcct
//
LOCUS       DAZABD010000175         1380 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000175 DAZABD010000000
VERSION     DAZABD010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1380
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.187"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(75..1169)
                     /locus_tag="ACHALG_002950"
     CDS             complement(75..1169)
                     /locus_tag="ACHALG_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HFN2588236.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            complement(1157..>1380)
                     /locus_tag="ACHALG_002951"
     CDS             complement(1157..>1380)
                     /locus_tag="ACHALG_002951"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2588237.1"
                     /translation="NNFIFLHNCSFPIYYFVIFNDNKIEGKYLLKLLEFIIYFKRKRK
                     GVDHLLCVCYNVSGYKNEKMQEEMVIWEN"
BASE COUNT          422 a          278 c          211 g          469 t
ORIGIN      
        1 ctataaaaaa acaaaataaa aaagcagaaa taactggcta tttctagcgg attatttctg
       61 caataactaa ttatctattt tttattttca attaatttaa agcttgacca tggtttcaaa
      121 aagtcaagga ggaataattc gtcatcggag attcttccta caacattgac acgtccgtcg
      181 tttttcatat ccttcaaggc gatttgtgtt tctcctttgt attgaccgta cccttcattg
      241 tcaatcagta catccccatg atgcatatct cgcgtattat gaggcgggaa atctttatct
      301 ttgtagtata cacgagtcat cgttgaacga aggatatatt ctgagcgatc ccctcgataa
      361 ctatgaaggt tatcaaacaa gcagattcgt tcgttttctg taataccttc ttccgtatct
      421 actttcaatg ttggataatc cgcgttaaat gcttctgcca tttctttcaa ttcttcttca
      481 cttgcgtacg catttccaat agaaatatca tcaatcaatc ctgtaaggat taaatgcttc
      541 acttgtgtcg caatttcaag atctcggtgg tcttccagcg tgcacaagcc atcttgcgta
      601 ggccaagggc caaactcagc agattgcgaa ttgacgaatg ccatcgtatt caaattatat
      661 tttctgaatt tttctgaaca aaaaacaaag tgttcgtatc ctaaaccaga gtatcgatga
      721 ggataaaaat tatgagaacc taataaatta tccgtgtttg gcgaataact catgatgttg
      781 tcaacgtaat tcgtgcccga actcatattg atctcgattt tgataccaaa aggattgcgc
      841 gtcatttttg cttcttctgc tcctgtgaat ccgatatcta agcggacccc gtaggcaccc
      901 atcttatgga aaaaagataa gtcatcgtaa gaaatctcca actgttcaaa taacgctgga
      961 ttgatatcaa ccattacttc catgcctagg ctattcgcgt aatcaactac ttctttaaat
     1021 tctgaaagaa ctttttcttt atcatcattg atctgcagca aactggtaaa aacccgttta
     1081 tacccatact tatgagctaa atccaagtac gctttgtcct tttcaaatgt tgaccgttct
     1141 ggataaatcg agattcctaa ttttcccata ttaccatttc ctcctgcatc ttttcgtttt
     1201 tgtatccact tacattatag caaacacaaa gtaaatggtc tacacctttt ctttttcttt
     1261 taaaataaat gataaattcc aataatttaa gtaggtattt tccctctatt ttgttatcat
     1321 taaagataac aaaatagtaa attggaaagg aacagttatg aagaaaaata aagttatttt
//
LOCUS       DAZABD010000176         1364 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000176 DAZABD010000000
VERSION     DAZABD010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.188"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(140..1288)
                     /gene="tnpB"
                     /locus_tag="ACHALG_002952"
     CDS             complement(140..1288)
                     /gene="tnpB"
                     /locus_tag="ACHALG_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element RNA-guided
                     endonuclease TnpB"
                     /protein_id="HFN2588238.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
BASE COUNT          393 a          242 c          216 g          513 t
ORIGIN      
        1 taaacctgac ggttcttaac gcttgaagtg ggagcttctt gggaatagag catacttgat
       61 agcgtatttc tttactaaca gcggtgtctc ttatttacca agctatcccc gtagttccta
      121 cggttcttga tgattggatc taagccaatc ccatagaatg tagtcttaga ccttcggtca
      181 gaatattgat actagcattg atatcacgat catgatgagt atgacaaaca ggacaagtcc
      241 attctcgaat ttcaagagat ttcttgccat ctttatgtcc gcactctgaa caaatctgac
      301 tagacggaaa ccatttatct actttgatga tttttcgtcc ataccagtca gccttgtatt
      361 gtaatttagt cacaaacttt gaccaagaaa catcagaaat acttttagct aatttatgat
      421 ttcgcaacat accttttgtg tttaagtctt caatacagat aatatcgtga tttttgatca
      481 tttctgtact caacttattc agaaaatcag tacgttgatt cattactttt tcatgcaatc
      541 tagctacttt gcgtttttgt ttttgataat ttttagcttc aaatagattg gtacctttat
      601 ttttagccaa taaagcacgc ctagacaatt tgcgttgttc acgctttagt ttcttttcca
      661 ttttggacgt gaatttatta ttatcaattt tttgtccatc agaaagaatc gcaaagtcca
      721 taatacctaa gtcaatacca attgtggaat tagttttagg taattcgata atttcttctt
      781 tacacaacaa agaaatatag tatttaccgc tagaatgacg tgatattgta gcagatttga
      841 taatcccttt tggttgtcta tgaagcttaa ttcttactaa tgacttcaat ttaggaactt
      901 tgatgaattt actatcaatc aaagcaactg taccattttg attattcgtt gtataactct
      961 gaacaggatt tttcttactt ttgaaacgtg gaaatccaac ggatttatcc cgaaaaaaat
     1021 tcttgtatgc tttatctaaa ttaagttggg cattggctaa agcaaggcta tcaatttctt
     1081 tcaaaaacgg aaattctttc ttgtatttag ctggtgtcgg aaaagtcatt tttttagaag
     1141 aatcattttt aacttcttca tacgcctttt ttcggtcatc aagcattaga ttgtagacct
     1201 tacggacaca accaaaagat ttggcaaaga agatttcttg ttcctctgtt ggatagattc
     1261 tgaatttgta tgcttttagt tgttccataa agttcaccta ctttctattt atgattgttt
     1321 ccttgatttt ggatatactt cttaatcaca tcaattggtg cgcc
//
LOCUS       DAZABD010000177         1228 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000177 DAZABD010000000
VERSION     DAZABD010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1228
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.190"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..>1228
                     /locus_tag="ACHALG_002953"
     CDS             82..>1228
                     /locus_tag="ACHALG_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HFN2588239.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGIT"
BASE COUNT          408 a          240 c          237 g          343 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcacca
//
LOCUS       DAZABD010000178         1203 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000178 DAZABD010000000
VERSION     DAZABD010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1203
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.191"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            22..>1203
                     /locus_tag="ACHALG_002954"
     CDS             22..>1203
                     /locus_tag="ACHALG_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFN2588240.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEESTLRSWKTDKSD
                     AHRLAQTHLKSQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFSNRLTPYALTLIRLFPHPDFVLASTRTKIKNKLINETRKKISANRAEQKADQII
                     HYAQCAYPAVEKDSIQCQKTIYYAELLQDLLEQKEALATQMIKKAEGSPCFLLYQTFP
                     GIGALTAALLLGELGDITRFKTHKQLNAFVGIDIRRYQSGKYTGQDRINKRGNPKARK
                     IIFFTIRNMIRQQRAAPNHIVDYYYKLKKQPIPKKEKVATVACMNKLLKCMHAMVRAH
                     TE"
BASE COUNT          404 a          268 c          223 g          308 t
ORIGIN      
        1 acttaaaaag gaggaaaaca aatgccttac attcttgcat tagatgtttc aatgggacac
       61 agctacagtg tcctatacaa agacgacacc tgcttatttg aagacgagat tgaacacact
      121 caaagaggat ttcatgcatt actcgaagag atccagcact tgcctgaacg tccattgatt
      181 gtttttgaag caacggggat ttattccaga ccagtcgaaa agttctttca agatcatcac
      241 ttttcttact gtttgttaaa cccgttagaa gcgaaaaaac agatggaaga aagcacctta
      301 cgcagttgga aaacagataa atcggatgcc catcggctcg cacagactca tcttaaaagt
      361 caacgtcagc caaaagaagt acaaagcaat ttttacttag agatgcgcga tttagcccgt
      421 ttttatcaag aaattgagaa gaaaatcact cgtctgcgca tggatttaca caactgtctt
      481 cagttgactt ttccagagct tgaacagttc ttttcaaaca ggctgacccc ttacgcattg
      541 acattgatcc gtctttttcc ccatccagat tttgtattgg catctactcg gacaaaaata
      601 aaaaataaac tgatcaacga aacacggaaa aaaatctccg cgaatcgtgc cgagcaaaag
      661 gcagatcaaa tcatccacta tgcacagtgt gcctatccag ctgttgaaaa agacagtatc
      721 caatgtcaaa aaacaatcta ttacgccgaa cttctccaag atttactcga acaaaaagaa
      781 gcactggcga cacaaatgat caaaaaagcc gaaggctctc cttgcttttt gttgtaccaa
      841 acgttccctg ggattggcgc actcacagcc gctttattgc tcggtgaatt aggagatatc
      901 acacgattta aaacccataa acaattgaat gcttttgtcg gaatcgacat ccgacggtat
      961 caatcaggta agtatactgg ccaagaccgc atcaataagc gtggtaatcc caaagcacgg
     1021 aaaattattt tcttcaccat tcgaaatatg attcgccagc aacgcgcagc tcctaatcat
     1081 attgtcgatt attactacaa attaaaaaag caacctatcc ctaagaaaga gaaggttgcc
     1141 acagttgctt gtatgaacaa gcttctgaaa tgtatgcacg ctatggttag ggcacataca
     1201 gag
//
LOCUS       DAZABD010000179         1193 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000179 DAZABD010000000
VERSION     DAZABD010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.192"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            143..400
                     /locus_tag="ACHALG_002955"
     CDS             143..400
                     /locus_tag="ACHALG_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2588241.1"
                     /translation="MSNLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
     gene            complement(632..1063)
                     /locus_tag="ACHALG_002956"
     CDS             complement(632..1063)
                     /locus_tag="ACHALG_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HFN2588242.1"
                     /translation="MIGEKMMDQKAFIESIDSTIDRLKKEIRFYNRVIGIGNIIKIVL
                     SASIPILIDQASEHRSLLLIVSIASAIITIIQSGMSAFNYQDKVQTSTELLMKIEKEK
                     LLYITKTSPYNKTDEENFHLIVTTLQTELNDIISDFNQVNN"
BASE COUNT          455 a          151 c          148 g          439 t
ORIGIN      
        1 ttttctgtcc acgttcattt tactccaaag ttttcctcat ttcaaaagaa aatcacagaa
       61 tacacgaaga agcgtttcta tttgtataaa ctatatcaaa gttctatact taaattatca
      121 aataaaaagg acgtgaagat aaatgtcaaa tttagaaaac aaagaagaaa aagttgtaaa
      181 caaaattgtt tctgttgtca ataaactgga taaagagtta gacgaacttg atacgttatc
      241 tgaaaatcca gaaaaaaaac ataacctaaa aaaatggtta gttgaacgaa aggctattca
      301 tgaaattaaa aaagttcttc atgaagctga taaatatgaa aaatatgatg aaaaagaact
      361 tgataaagaa tttaaagaaa tcaatgactt attactataa ttgatttata cgactcattt
      421 ttgagtcgtc agactgcaaa caacactcta aatttaggag tttgtctaca gtctgaataa
      481 attttaaatt tattgtccat aatgttatta tgaatttata gcattaagaa aagagataag
      541 actttacatt tttttaggaa tgtaaagtct tatctctttt cttaattgta caattagtat
      601 tatctgcact actttctaat taaataaatt tttagttatt aacttgatta aaatcagaga
      661 taatatcatt taattcagtt tgaagggttg taacgattaa atggaaattt tcttcatctg
      721 ttttattata tggggatgtt ttcgttatat ataacaactt ttctttttca attttcatta
      781 ataactctgt cgaagtctgt actttatctt gataattaaa tgcagacata ccactttgta
      841 taattgtaat tattgctgaa gcaattgaaa caatcaatag taaagacctg tgttcggaag
      901 cctgatcaat taatattggg atactagctg ataagactat ttttataata tttccgatac
      961 caattactct attataaaat cgaatttctt tttttaatct atcgattgta gaatcaatag
     1021 attcgataaa cgctttttga tccatcattt tctctccaat cattttatta aaatcattaa
     1081 caaaaatatt ataataggat ttaagtaagt tctaactaat cacatataaa aataaaaaaa
     1141 catttgtatt ttatcaaagt tttattatac ttgtcaattt gtgtacatcc taa
//
LOCUS       DAZABD010000180         1089 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000180 DAZABD010000000
VERSION     DAZABD010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.193"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..978
                     /gene="bsh"
                     /locus_tag="ACHALG_002957"
     CDS             4..978
                     /gene="bsh"
                     /locus_tag="ACHALG_002957"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFN2588243.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGETKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          382 a          135 c          187 g          385 t
ORIGIN      
        1 actatgtgta cgtctattac ttatgtaaca agtgatcatt attttggaag gaattttgat
       61 tatgaaatat cttacaatga agtagttact gttactccaa gaaattataa gttgaatttt
      121 cgaaaggtaa atgatttgga tactcattat gcaatgattg gtattgccgc tggtatagct
      181 gactaccctc tttattacga tgcgacaaat gaaaaaggat tgagtatggc tgggctaaat
      241 ttttctgggt atgctgatta taaagaaata caagaaggga aagacaatgt atctcctttt
      301 gaatttattc cttggatttt aggacaatgc tcaacagtag gagaaactaa aaaattgtta
      361 aaaaatatca atttagcaaa tataaattat agtgatgaac ttcctttatc ccctttacat
      421 tggctattag ctgataaaga aaaatcaatt gtcattgaaa gtatgaaaga tggacttcat
      481 atatatgata accctgtggg cgttcttacc aataatcctt catttgacta tcaattattt
      541 aatttaaaca attatcgtgt cttatcgagt gaaactccta aaaataattt ttcaaatcaa
      601 ataagtttga atgcctatag ccgcggtatg ggagggatag gcttgcctgg agatttatcc
      661 tcagtatctc gttttgttaa agcgactttt acgaagctga attctgtatc tggagattca
      721 gagtcagaaa gtattagtca atttttccat atcttaggtt cagtagaaca acaaaaaggt
      781 ttgtgtgatg ttggtgatgg aaaatatgaa tatacaattt attcttcttg ttgcaatgtt
      841 gacaaaggaa tctattatta tcgaacatat gaagacagtc aaattactgc aattgatatg
      901 aataaagaag acttagatag tcataagtta attagttatc caattataga aaaacaacaa
      961 attaaatata taaattagtt aatgtgttgt gattgattat ttaatataga tataatacaa
     1021 aaaggcaaag atattttcaa aacatttttt cttgatgtga tatccttgtc ttttttgtaa
     1081 tgtttttaa
//
LOCUS       DAZABD010000181         1044 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000181 DAZABD010000000
VERSION     DAZABD010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.194"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..464)
                     /locus_tag="ACHALG_002958"
     CDS             complement(<1..464)
                     /locus_tag="ACHALG_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFN2588244.1"
                     /translation="MKKWLFGFLGVTLIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVM"
     gene            complement(524..733)
                     /locus_tag="ACHALG_002959"
     CDS             complement(524..733)
                     /locus_tag="ACHALG_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2588245.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVAQYINDMQLT
                     GKEKACLLGSMKNFEQLRRTYRIHF"
     gene            complement(800..1039)
                     /locus_tag="ACHALG_002960"
     CDS             complement(800..1039)
                     /locus_tag="ACHALG_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2588246.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKEVFKALSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
BASE COUNT          284 a          234 c          135 g          391 t
ORIGIN      
        1 tccatcacct cccctaatga caacttttga atatcagaaa ataaaacacc tggcatttcc
       61 atgttatgac ctgctaggac atagtttcct ttgcccattt cggcatcttc ataaaaataa
      121 gtagccgctc caagactcaa agtgtattga tttgcccctt taaaaattgg caatgctaaa
      181 ccaagtgcag gaatctgaat ttgtgcaaat acttgtttat tgacgcccat ttcgattgct
      241 tctttttgta tggacaacat ttgccccaaa gacatatcat tattgacgtt cgttgcatcc
      301 tccttataac tctttgcatg tgcattgatt tgatctacgg gcatcttttt ttctatattt
      361 tgcttaaaca cctctccttc atgtttttgg tagctaacat agccaaacac actgcaaaag
      421 acgattaaag tgactcctaa aaaaccaaat aaccattttt tcatccatct gcctcctttc
      481 agacaaaaca aaaaaacttt ttctcatttc catagaaaaa gttttaaaaa tgaattctat
      541 aagttcgtct caactgttca aagtttttca ttgaacctaa caaacaagct ttctcttttc
      601 ctgttaactg catatcattg atatattgcg cgacagataa tcttttaagg tgtttttgct
      661 tcatcgtttt tagtaacttt tcttccattt ctttcgacac tttttttctg ttttcgattt
      721 cttcataatt catgagtttt cctaccttcc ttgttttttg caaaaataaa aaagcctatg
      781 atcacatacg ctctacactt cagtcttggg ttatcttttt ctttcgttta attactggcc
      841 gtgctggttt caaatccttg attccattat gggttctcca ataatcgaat tcttttcgca
      901 atctttcttt ttccatatct gttcctttag ataaggcttt gaatacttct ttaggtgaat
      961 aaccacgagc taacagctgt acataccttc cttttgtcaa agtatccata tccatcgcct
     1021 ctaaaactgg tcgcgccatc tact
//
LOCUS       DAZABD010000182          955 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000182 DAZABD010000000
VERSION     DAZABD010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..955
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.195"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>955
                     /locus_tag="ACHALG_002961"
     rRNA            <1..>955
                     /locus_tag="ACHALG_002961"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          285 a          192 c          276 g          202 t
ORIGIN      
        1 caaaccagca agcttgcttg ttggggttgt aggactccaa tatggtagtt ctttcagata
       61 gtcgaatgac ttggaaaagt cagtcaaaga gggtaaaaac cccgtagacg aaatgtggaa
      121 gacacctagg aggatcctga gtacggcgga acacgagaaa ttccgtcgga atccgggagg
      181 accatctccc aaggctaaat actccctagt gaccgatagt gaaccagtac cgtgagggaa
      241 aggtgaaaag caccccggaa ggggagtgaa atagaacctg aaaccgtgtg cctacaacaa
      301 gtcaaagccc gttaatgggt gatggcgtgc cttttgtaga atgaaccggc gagttacgat
      361 tgcatgcgag gttaagttga agagacggag ccgcagcgaa agcgagtctg aatagggcgt
      421 ttgagtatgt agtcgtagac ccgaaaccat gtgatctacc catgtccagg ttgaaggtgc
      481 ggtaaaacgc actggaggac cgaacccacg tacgttgaaa agtgcgggga tgaggtgtgg
      541 gtagcggaga aattccaaac gaacttggag atagctggtt ctctccgaaa tagctttagg
      601 gctagcctcg gaattgagaa tgatggaggt agagcactgt ttggactagg ggcccatctc
      661 gggttaccga attcagataa actccgaatg ccattcattc atatccggga gtcagactgt
      721 gagtgataag atccatagtc gaaagggaaa cagcccagac caccagctaa ggtcccaaaa
      781 tatatgttaa gtggaaaagg atgtggggtt gcacagacaa ctaggatgtt ggcttagaag
      841 cagccaccat ttaaagagtg cgtaatagct cactagtcga gtgaccctgc gccgaaaatg
      901 taccggggct aaacatatta ccgaagctgt ggagtacacc tttaggtgta ttggt
//
LOCUS       DAZABD010000183          953 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000183 DAZABD010000000
VERSION     DAZABD010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..953
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.196"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>953)
                     /locus_tag="ACHALG_002962"
     CDS             complement(<1..>953)
                     /locus_tag="ACHALG_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1476 family transposase"
                     /protein_id="HFN2588247.1"
                     /translation="VKEKLTILELKRTRFLCHDCGRTFSAKTDLVDEHHQLTKELKQA
                     ILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVLCMDEFKSMKSV
                     SGSMSFICVDGTTNQLFTILEDRRLYKLTQYFLRFPRKARLKVKYLVMDMNASYCQLL
                     KTVFPNAEIITDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYRRLKRYWKLLLK
                     DSTTLEPLKRHYHRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLRYYFVKRDTEGF
                     QETLKTISSTLPQSFKKKFTIFHRYQSGISNAFKVSHSNGVT"
BASE COUNT          266 a          179 c          210 g          298 t
ORIGIN      
        1 gtgacaccgt ttgagtggga cactttaaag gcatttgaaa tgcccgattg gtagcgatgg
       61 aagatcgtga atttcttttt aaacgactga ggtaacgtag atgaaattgt tttaagggtt
      121 tcttgaaagc cttccgtatc tcgtttaacg aaataatagc gtaataattg acaaaaatga
      181 tacgctgttc gcaattcttc gttgtagctt aacagttcat cgacgatttc tgtttgacta
      241 attggtcgtt taaacagtcg atgataatgc cgtttgagtg gctccaatgt cgtggaatct
      301 ttgagtaaga gtttccaata tcgcttgagc cgacggtact tcttctgatt ttcagagtgt
      361 gaggttcgaa aacgattcat gatctggaca cgtaattgat tgaaactgcg attgatgtgt
      421 tggacaatat gaaagcggtc agttatgatc tccgcatttg gaaagaccgt tttaagcaac
      481 tgacaatagc tggcattcat gtccatcact aggtacttga cctttaaacg ggcctttcta
      541 ggaaagcgca ggaagtattg agttagtttg taaagtctgc gatcttcaag gatcgtaaat
      601 agctgattgg ttgtcccatc aacacaaata aaactcatgg aaccagaaac agacttcatt
      661 gatttgaact catccataca caacaccgtc ggaaggaagt tcatacgtgg ctggtaatgc
      721 ttcgttgctt cacgcaagat ccgtgtcacc gtcccatccg agacgaagta cttcttagcg
      781 attagtttac gtgattgatt ttcgtacagt tccatgagaa tcgcctgctt cagttccttt
      841 gttagttgat gatgttcatc aactaagtcc gttttcgcag aaaaggtccg tccacaatca
      901 tggcagagaa aacgggtacg ttttaattct aaaatagtta acttttcttt gac
//
LOCUS       DAZABD010000184          907 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000184 DAZABD010000000
VERSION     DAZABD010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..907
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.197"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            167..364
                     /locus_tag="ACHALG_002963"
     CDS             167..364
                     /locus_tag="ACHALG_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFN2588248.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            366..677
                     /locus_tag="ACHALG_002964"
     CDS             366..677
                     /locus_tag="ACHALG_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFN2588249.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPSPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            781..>907
                     /locus_tag="ACHALG_002965"
     CDS             781..>907
                     /locus_tag="ACHALG_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HFN2588250.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFS"
BASE COUNT          363 a           87 c          160 g          297 t
ORIGIN      
        1 tgcattctca aaaaatatta aagaatatac atagtttata tacttaaaag tgattaaatt
       61 cttattcagg aattattttg ctacgtttca agaaggaatt gttttttgaa atttaatgaa
      121 tgttagaata atagtctaaa aacattaatt taggggtgat tagattatga aacatttaaa
      181 aattttgtct attaaagaga cacaacttat ctatgggggt accactcata gtggaaaata
      241 ttatggaaat ggagtgtatt gcactaaaaa taaatgtacg gtcgattggg ccaaggcaac
      301 tacttgtatt gcaggaatgt ctataggtgg ttttttaggt ggagcaattc cagggaagtg
      361 ctaaaatgaa aaaaaatgct aagcaaattg ttcatgaatt atataatgat atatctataa
      421 gtaaagatcc taaatattct gatattcttg aggttttaca aaaggtatat ttaaaattag
      481 aaaaacaaaa atatgaatta gatcccagtc ctttaataaa tagattggtg aattatctat
      541 attttactgc ttatactaat aaaataagat tcactgaata tcaagaggaa ttaataagaa
      601 atttgagtga aattggaaga actgctggaa taaatggttt atatcgagca gattatggag
      661 ataaatctca attttaatga tacttataat attaaatatg tctcaaaatt ttttgattag
      721 aaaaataggt atattgaatc agtgacataa aagtatttat agtttgtagg gggaatcgta
      781 atggaagaaa aaaatagact aaatgctaaa caatgttcag atcaagagct taaaaaaatt
      841 aaaggaggtg ctggaacaaa acctcaagga aaaccggcat caaatttagt ggaatgtgtt
      901 ttttcat
//
LOCUS       DAZABD010000185          900 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000185 DAZABD010000000
VERSION     DAZABD010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..900
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.198"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            28..>900
                     /locus_tag="ACHALG_002966"
     CDS             28..>900
                     /locus_tag="ACHALG_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFN2588251.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDEFFIQSVASKRNN"
BASE COUNT          312 a          155 c          169 g          264 t
ORIGIN      
        1 ccaaagaatt ataaggagtg agtacaaatg acctataccc atcttacaac ggatgaactt
       61 gtaataatag agtcttattt caaaatgaat caatctgttg ctaaaactgc ccattgcttg
      121 aatcgttcaa gacaaacgat ccataaagta tatctgttct tcaagcaagg aaaatcagcc
      181 ttagaatatt atcaacagta taagaaaaac aaatcaaact gtggtagacg tccgcttgtt
      241 ttacccgagg aacaatcaga atatattcaa agaaaggttg ttcaaggatg gacacccgat
      301 gtgattgttg gtcgtgcagc gtttcctatt agttgttctg ctcgtaccat ttatcgtatg
      361 ttcaaaaagg ggctatttga ttcttctgac ttaccgatga aaggcaagcg taaaccgaat
      421 ggacatcaag aaagacgtgg aaaacaaact ttccgccgct ctattcatga acgtgaaaag
      481 gattatagcc aattctcaaa tgagtttggt caccttgaag gtgacactat cgtaggtctg
      541 aaacataaaa gtgctgtaat taccttagtt gaacgattat caaaagttat catcacattg
      601 aaaccgtgtg gtagacaagc gattgatatt gaaaaaaaat taaatcaatg gtttgaatct
      661 gtaccgaaaa acctattcaa atccatcact tttgattgtg gaaaggaatt ttcaaattgg
      721 aaacagatca gtaatgtcaa tgatattgcc atttatttcg ctgatccagg aacgccgtct
      781 caaagaggcc taaacgagaa ttctaacgga ttgttacgta gagatggttt attgaaatct
      841 atggatttca attcagtaga tgaatttttt attcaatctg tcgcatctaa acgaaataat
//
LOCUS       DAZABD010000186          888 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000186 DAZABD010000000
VERSION     DAZABD010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..888
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.199"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..254)
                     /locus_tag="ACHALG_002967"
     CDS             complement(<1..254)
                     /locus_tag="ACHALG_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFN2588252.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSART"
     gene            complement(254..517)
                     /locus_tag="ACHALG_002968"
     CDS             complement(254..517)
                     /locus_tag="ACHALG_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFN2588253.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            751..>888
                     /locus_tag="ACHALG_002969"
     CDS             751..>888
                     /locus_tag="ACHALG_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HFN2588254.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LK"
BASE COUNT          285 a          176 c          119 g          308 t
ORIGIN      
        1 gtccttgctg aataaatttc aaccacgtgt tcatcttcat ctaccttata aacaacccgg
       61 tgttgtctat tcaatcttct agaataccta ccttcatgtg taggttttaa cttctcaaat
      121 gattgggtag ggagataggg atcttccttt aaagtctgaa taaccttctc aaattgaggt
      181 ttcaaattag actgtttgat tttctttaaa tcagctttag ccgagttttt aatcatcaca
      241 gaatagttat tcattataag ttatcccaat caatttcatc aacattagta aatccactat
      301 catctttttc tcgcttacga acaacaccca aagtgcctat ttgctcaagt aatagcgttt
      361 ctttgattgc tttccaatca tccaaaccaa taagcaccgc attattttca ctgcgctcac
      421 taatgatttc aatttccgta tgattctcat ttacatcttt caacaactta taaaaatttt
      481 tccgtgctga gctaggattt aagactgttt ttgccatatc tactccccct ctaatttata
      541 tctccattct aacgtactta attaagtacg taaattcgat tgttttgttt aaattggagt
      601 accaaaacag cattgtaaag tgttatccgt ttcctttata cccgtggtgc tagttaattt
      661 tttcctagag gaaaatgtct aaacatgttg gtaaatcaac aaaaaaacgc atagagtagt
      721 ctcaaaactc ccaagaaatg aggactacat atgcgttcat caaaatatac tgaacattat
      781 acagatactt ttataacatt caaggaaatt atgagaactg tttctaacat ataccacaat
      841 tgtgtaccag ataagattaa aaaccgaaga aatactgatc aactgaag
//
LOCUS       DAZABD010000187          883 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000187 DAZABD010000000
VERSION     DAZABD010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..883
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.200"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(248..601)
                     /gene="tnpB"
                     /locus_tag="ACHALG_002970"
     CDS             complement(248..601)
                     /gene="tnpB"
                     /locus_tag="ACHALG_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFN2588255.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(591..785)
                     /locus_tag="ACHALG_002971"
     CDS             complement(591..785)
                     /locus_tag="ACHALG_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2588256.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
BASE COUNT          253 a          174 c          158 g          298 t
ORIGIN      
        1 cgtccaaata atttgttggt taaataattg accttttcat tcaatagttg gatttcagtt
       61 gtaagattct gaatggtttg ttgttgtgct tgattggttt tatgggcttc ttgaagcaac
      121 tgaagcaacg tatctgtagt ttccaaaatt tttcaccacc ttgaatcgaa ctgattttgt
      181 tacagaatac cacaagaaac gcacaagaaa taggggttca acacattttt tgtgttaacc
      241 ccccagctta aaaaactcct ttttttcctg gcaaaatagc tcgaggttga tcgatactta
      301 atccttctaa caaccatcga acctgttggt tcgttaaggt tcgaacttcg tctttcgtcc
      361 gaggccattt taacttgccg ttttctatcc gcttataaca gagaagaaaa ccatcaccgt
      421 cccaatagag aagtttgaaa cggtcttgac ggttcccgca aaataaaaaa acggcatctt
      481 cgtaaagctc gagttgatac tcttgttgaa taagactggc aagaccatca attccttttc
      541 gcaaatctgt ttttccgcac acaatataaa tatgtttggt ttgaagtaaa tcataaagca
      601 ttggacaaca gttctttcaa aatagcttga agaatataat tgttaatacc gttatagatc
      661 aaaacttcag aaccattctc tcttttgatg cgcatcgcta acttggcagc cgcgggttta
      721 cctttttctg gtgcactctt ttcgtttcct aattggacag gaacaatctc atttaattta
      781 tacatagaag acctccagac ttcttgatag ctcaagtata tcttgaggtg catttgttag
      841 taaggtactg ttctatgggg cgcttacgtt aaaaatgttt tgc
//
LOCUS       DAZABD010000188          877 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000188 DAZABD010000000
VERSION     DAZABD010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..877
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.201"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..624
                     /locus_tag="ACHALG_002972"
     CDS             85..624
                     /locus_tag="ACHALG_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2588257.1"
                     /translation="MWSLVGIVLIIFTVTAATEANYSHKIQSYKGRLVMTKKDVTRYT
                     SSRTGGLYDYAPLDALKYKDHLKKHEVKVGDRWENASHKAYNSKIVYTVEDADEQNIT
                     LPVFNYWGTVVKVNGKKSTIENDNGLVSIKGTGKRTIVEVSSTYPTTMILSLLISSIT
                     FIFLFTRYLIRCCGEMKID"
BASE COUNT          335 a          106 c          153 g          283 t
ORIGIN      
        1 agaaccttgg gttttcaagt gctttttggt tcattaatct taaccatagt gtttttaaag
       61 tggaaaacaa gcaacaaaaa aagtatgtgg agccttgtag gaatagttct tattattttt
      121 actgtgactg cagcaacaga agcaaattat tcgcataaaa ttcagtcgta taaaggtcgt
      181 ttagttatga ccaaaaaaga tgttacacgt tatacatcct caagaactgg aggcctatat
      241 gactatgcac cattagatgc attgaaatat aaagatcatt taaagaaaca tgaagtaaaa
      301 gttggtgatc ggtgggagaa tgcatctcat aaagcatata attccaaaat agtctataca
      361 gtagaagatg ccgatgagca aaatatcaca ttacctgtat tcaattattg gggaacagtt
      421 gtgaaagtaa atgggaaaaa gagcactata gaaaatgaca atggtttagt gtctattaaa
      481 ggtacgggga aaagaactat agttgaggtt tcatctacgt atccaactac gatgattcta
      541 tctttattaa ttagttcaat aacttttata ttcttattta cacgttattt aataagatgc
      601 tgcggtgaaa tgaaaataga ttagatagat attataaata tgttgattct attttcatag
      661 caatggagaa aatacattag caatatataa aagaaggtat caagagtcac agtgaattta
      721 ttccgaaaag tgaaaagagc aaatataaaa tttaatgaaa atatagaact aaataggaaa
      781 acgtagacgt aattttctac gttttccttt tcatatatat taaatattac ggaaaattat
      841 ataaactaat ttagtgtttt gtttgtaatt cctaatt
//
LOCUS       DAZABD010000189          845 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000189 DAZABD010000000
VERSION     DAZABD010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..845
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.202"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..275)
                     /locus_tag="ACHALG_002973"
     CDS             complement(<1..275)
                     /locus_tag="ACHALG_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008177227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HFN2588258.1"
                     /translation="MMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFEFIYPIV
                     EATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRW"
     gene            complement(440..>845)
                     /locus_tag="ACHALG_002974"
     CDS             complement(440..>845)
                     /locus_tag="ACHALG_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HFN2588259.1"
                     /translation="TPENHFSNQISLNVYSRGMGGLGLPGDLSSVSRFVKATFTKMNA
                     ASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSCCNVDKGIYYYRTYEDS
                     QITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
BASE COUNT          273 a          164 c          141 g          267 t
ORIGIN      
        1 aaccagcggt aagcgacatt ggtatcgact tccttaatgg tttgtcgcat ggaacggata
       61 ccgaataaat actgaataaa caccaatttg atcagaatca ctggatcgat acttgggcga
      121 cctaaatcgg agtaagtggc ttcgacgatt ggataaataa attcaaaatc aagcgcggca
      181 tcaatctttc gaacgagatg atcttgagga actagatcat caatcgtaag catcgcaaat
      241 tgatttctac cattcgtatt gtttttagac atcatagcta tcactccttg cattcgtttt
      301 cataaatcta ttataaaaga aaagactgta gatatcatcc ataaaatgga ctttgtctac
      361 agtctgagac acgacctaaa gatcgtgtct aattatgact gtatcagaaa atatgatacc
      421 aaacggatct attcattaat taattgacat agtttatctg ttgctctttt atgactggat
      481 aactgaccaa ttcatgacga tccaagtctt ctttattcat atttacagca gtaatttgac
      541 tgtcttcata cgtacgataa taataaatcc ctttatcaac gttacagcaa gaagaataaa
      601 tcgtatattc gtattttcct tctccagtat cgcatactcc tttttgttgc tcgactgagc
      661 ctagtatatg aaaaaattga ctgatacttt cagattctga atcacctgat gcagcattca
      721 ttttagtaaa tgtggctttt acaaatcgag aaacagaaga gagatctcca ggtaagccca
      781 atcctcccat cccacgacta taaacattca agctgatctg gtttgaaaaa tgattttctg
      841 gtgtt
//
LOCUS       DAZABD010000190          823 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000190 DAZABD010000000
VERSION     DAZABD010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..823
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.203"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..698)
                     /locus_tag="ACHALG_002975"
                     /pseudo
     CDS             complement(1..698)
                     /locus_tag="ACHALG_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
BASE COUNT          273 a          132 c           83 g          335 t
ORIGIN      
        1 atatcagtta aatcattata aatagtttca cgcaacaata tatactcttc taatacattt
       61 tgtttattat caatagtaca ttcactaata tctgtataat ctaaaccgtg catttgtctt
      121 aaaaaactgg caatatctcg ttttaacaaa ttttgttctt cttctgacat agtagaataa
      181 atttctggtg ttaaaaaagt tcctttaatt tctttataac ctagtataga taattcatca
      241 ctaatatacg aatattcaat attaggaatt tttacattag tttctaaatt tgtatttaaa
      301 aaattatata ttgctttttc ttttgcataa ccttttttct tattagtact aaattttgtt
      361 ttaaaaatgt attcattatt aactaaatat gccacactat cataaccact accgattatt
      421 tcaatactat ctactttgaa attatcaaag taatgctcaa ttaaatattt cattgcctta
      481 acatttgtgg cattatcatc atatctatat tccattaaat aacaatcttc ttttttgccc
      541 tcgtgtaatt catgttctgg caaatcttca ataattctaa aaccagattt ttggtatgcc
      601 cttattgctc ttggattatt tttatgaggg tctaaaataa ctgcattagc atttctttct
      661 tttttcaaaa attcaaaaat caatttaata tatcttgtac ggttctgttg caaagtttta
      721 aataaagaat aaaatccctt acggtatcta tgatttaagc tgggattccc aataatacct
      781 tgatttcagt acagaccgaa aacccgaaga gagtgccttc ttt
//
LOCUS       DAZABD010000191          787 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000191 DAZABD010000000
VERSION     DAZABD010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..787
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.204"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            106..381
                     /locus_tag="ACHALG_002976"
     CDS             106..381
                     /locus_tag="ACHALG_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2588260.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            complement(410..>787)
                     /locus_tag="ACHALG_002977"
     CDS             complement(410..>787)
                     /locus_tag="ACHALG_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFN2588261.1"
                     /translation="GRKALDIETALNQWFSRFPKNFFKSITFDCGKEFSNWKAISNQH
                     DIDIYFADPGTPSQRPLNENSNGILRRNGLPKSMDFRKVNQTFISSVSNQRNHIPRKS
                     LNYRTPIEIFLSYVQEAFYSSLI"
BASE COUNT          291 a          122 c          127 g          247 t
ORIGIN      
        1 aaataaccac tatttgattt atatatattt cagtatcctt cttgttttca tataaccaaa
       61 ggcgctatga tttccatatg aaaaaaataa agtcaggtga aaaaaatgaa acttgaggat
      121 aaaaaagtca ttttgaagaa aatcaaacga ctaccagacc aaatagatgg ctatacgatc
      181 tgttctttca aaaaagacag gagtataaaa gcttatacgc aaaacacaga aaaaggaata
      241 atctatttcc tacatgaaga aggttacgat acccaaacct ttatgtttac tgaaaaaaaa
      301 gaatttcata aacagatgaa aaaactgatt gaaaaagaat ttccaagaag tcataaactc
      361 tatgtgaatc agcaatttta acttctaact gttctttatt tatgatttgt caaattaagc
      421 tagaataaaa tgcttcttgt acatagctca aaaatatttc gattggtgtt ctgtaattca
      481 atgattttct tggaatatga ttacgttgat tgctgacact ggaaataaat gtctgattca
      541 cttttctaaa atccattgat ttcggcagtc cattacgacg cagaatcccg ttagaattct
      601 cgtttaatgg gcgttgagaa ggtgttccag ggtccgcaaa atatatatca atatcatgtt
      661 ggttactaat ggctttccag ttagaaaatt cttttccaca gtcaaacgta atagatttaa
      721 agaagttttt agggaagcga gaaaaccatt gattaagggc agtttcaata tctaatgcct
      781 tacggcc
//
LOCUS       DAZABD010000192          766 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000192 DAZABD010000000
VERSION     DAZABD010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..766
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.205"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..638
                     /locus_tag="ACHALG_002978"
     CDS             <1..638
                     /locus_tag="ACHALG_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFN2588262.1"
                     /translation="VSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFGEDLD
                     LPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVYPKLI
                     ADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEIKEKQ
                     DVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
BASE COUNT          264 a          134 c          144 g          224 t
ORIGIN      
        1 atgtatcaca agtagactta aaaactgctt tgatctattc cgataatata tatgcggcac
       61 aagaaacgtt gaaaatgggt gagaaaaaat ttcgtacagg cttagataaa ttcatttttg
      121 gtgaagacct tgatttgcca attagtatga atccagcaca aatttctaat gaagatagct
      181 ttaactcaga tatcttgcta gctgatactg gatatggaca gggcgaactt ctaattactc
      241 ctatccagca agcagcaatg tattctgttt ttaccaacaa tggcacactt gtctatccta
      301 aattgattgc agataaagag acaaaagata agaagaatgt catcagcgaa acagcagtac
      361 aaacgatcgt gccagatctg agagaagttg tgcaagatgt aaatggtaca gcacattctc
      421 tttctgcttt agggattccg ttggcagcga aaactggtac agcggaaatc aaagaaaaac
      481 aggatgtaaa ggggcaagag aacagtttct tgtttgcttt caatcttgat aaccaaggtt
      541 atatgatggt cagcatgttg gaaaataaag aagatgatga ttcagcaacc aaacgagcat
      601 cggaactatt gcaatacctc aaccaaaatt atcaataaaa aattgctttt taagtacagt
      661 aaaaggactg tggcgttatc gtcacagtcc ttttttcaaa taaacggttt tcacgatgtc
      721 tctttcacaa cttctttttg atttactcac acttcataaa cttttt
//
LOCUS       DAZABD010000193          766 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000193 DAZABD010000000
VERSION     DAZABD010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..766
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.206"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            661..>766
                     /locus_tag="ACHALG_002979"
     rRNA            661..>766
                     /locus_tag="ACHALG_002979"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          315 a          124 c          135 g          192 t
ORIGIN      
        1 gaattcctta ggcagctttt gctgtgtaga aaggaataaa taataacgaa agttcaaaag
       61 ctaatgccga ctattctaga cattaaagaa gacgaaaacg aacgataaag aaagaaaagt
      121 tatgaataat cgtttattga aaaaaacttg aaaaaataca aaaaattcgt tgaccaagct
      181 aaatttagtt ggtatattat tacttgtcca aaagacagaa gaaaatattt attcaaaaac
      241 ataacaaaaa agttattgac atgttactaa cgacatgata agataactga gtcgttaaaa
      301 aagaaccact tgcgaagctt gattctatcg aaaaaaactt caaaaaagtt cttgacaaat
      361 aacaaacgat aagttatgat aaatgagttg ccgaaagatt tcgaaaaacg aaatcgaaaa
      421 aaacttttaa aaaagagttg acaatcgctt gtctctctga tatgatataa aagttgctac
      481 ggcagccaaa aatgaatcgg gaaacactct cgatgaagta gacctttgaa aactgaacaa
      541 agtaagacaa accaaatgtg tagggcgtct tgattcaatt caagacaaca aacattttta
      601 acaagcaagc aatatgctag caaacaaatt gagcttaaca atcgcaagat tgttcgaact
      661 ttttatgaga gtttgatcct ggctcaggac gaacgctggc ggcgtgccta atacatgcaa
      721 gtcgaacgct tctttttcca ccggagcttg ctccaccgga aaaaga
//
LOCUS       DAZABD010000194          735 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000194 DAZABD010000000
VERSION     DAZABD010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..735
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.207"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(93..668)
                     /locus_tag="ACHALG_002980"
     CDS             complement(93..668)
                     /locus_tag="ACHALG_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
                     /protein_id="HFN2588263.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
BASE COUNT          245 a          155 c          130 g          205 t
ORIGIN      
        1 aatggaaagt tttgtctcgc ttaatttgac aaataataaa atgaaaaaac agagccagat
       61 tagcattatt ttgctaatcc agctctgttt attcagccta tttttctttt tgaatatatc
      121 aagcgttggg gcaacatagt gatgactaat gaaacaacga cgatagacaa caagcgatca
      181 aggtagtctg tacccgcctg taccaagatc acacttgttg tctgacttaa acctaaccca
      241 tgtaatagct gaacgatcat actggatcct gaggaggtga tcccgccaaa taactgaacg
      301 gtaatgatcg aagaaacgat cgttccaggt accgacaaaa tcaaagcgag taataacagt
      361 ttcaagctgt ttttatcttt gccgagatat cttggcaaaa tcaatccagc aagcaagcct
      421 gtgagaattt ggattggcga ataatacaga gaaaaaatat ctgtagtcac tccacttaaa
      481 ataccgctgg acaatcctgt cagcattcca ggtacaggcc caagcaatgc accagcaaaa
      541 atcgttccga tagaatcgag atagattggc aaacgaagcc ataaagcaat cgttcctcca
      601 atataattga tcccaataca caaagccatc actgtaagta cgcgagttgc cattttgttt
      661 ttattcattt tgttagccca tctttaacct ttccaaatct ttcataatcg tttcttcttg
      721 tgcatttaaa agaac
//
LOCUS       DAZABD010000195          671 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000195 DAZABD010000000
VERSION     DAZABD010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..671
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.208"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            36..>671
                     /locus_tag="ACHALG_002981"
     CDS             36..>671
                     /locus_tag="ACHALG_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFN2588264.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFS"
BASE COUNT          197 a          131 c          131 g          212 t
ORIGIN      
        1 attaatttgg ctaccaattc ataggaggat tcaagatgtc tcatactcag cttatcaaag
       61 ataccctaaa tattctagac ctaaatatct atttcgagga aaattgttta actaaagaaa
      121 aatacaaagg gcaaatttgc ttgatttaca aaggaactct tctgtacaag ccagaagagt
      181 gttcccattg tctttgtgtc gtcccctcta ggatcattcg ttgggggacg acaaccgttc
      241 ggttgttact gaatgatgtg tctgagtatc gcacttatct ggagctgaag aaacaacgat
      301 ttaaatgtaa gtcttgccaa agaacattcg tagcggatac ctctgttgct gaaaaacact
      361 gtttcattag tcaaaaagtt cgttggtctg tcattgctcg cttaaaagag aatacttcca
      421 tgacggaaat cgctcgtcag aaaaatattt ccacttcttc ggtctatcgt gtcatgaagc
      481 gattctaccg cccattgaat cctttcaaac agactttgcc aaaggtctta tgctttgatg
      541 aattcaaatc ggttcgaagt gttgccagtg caatgagttt tatcatgatg gacggacaaa
      601 gccatgcatt attggatatt gtcgaaaatc gacgcttgcc ttatttagaa cgctattttt
      661 ctcggttttc a
//
LOCUS       DAZABD010000196          641 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000196 DAZABD010000000
VERSION     DAZABD010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..641
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.210"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..284
                     /locus_tag="ACHALG_002982"
     CDS             <1..284
                     /locus_tag="ACHALG_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFN2588265.1"
                     /translation="LIQDDLHHTHRLWLMNKINLYFGHSYVFAFYDYRTNITEVDLVI
                     SNYYIDTGKTPLLLMKNIPTERNWRLFEKTIYQLKKEKEVVKSAFCPEY"
BASE COUNT          218 a           89 c          119 g          215 t
ORIGIN      
        1 ttttaataca agatgattta caccatacgc atcggttatg gttaatgaat aaaattaatc
       61 tgtattttgg gcattcttat gtttttgctt tttatgatta caggacaaat ataactgaag
      121 tagatttggt tatttctaat tactacatcg acactggaaa aacgccatta ctgttaatga
      181 aaaatatacc taccgaaaga aactggcgtt tgttcgagaa aacgatttat caattgaaaa
      241 aagaaaaaga ggttgtgaaa tcagcgtttt gtcctgagta ttagagaaac aaacggaaaa
      301 atagtttttc atattttgcc gtttttgagc taataccgag ggacagctga ttgaacacca
      361 tttattgaga gattgtgaaa aaactgtcct gcatcaagta ataagaacag ccaagtaaaa
      421 acagcttctc atgtttgttg aatattgtga cttgtgaccg aggagttggc aagagaacac
      481 cgtttattcg gaaaaaggga ctgtgacact acatgtcaca gtcccttttt gattgaatta
      541 tctcatttgt tttctaattt tttctttatt tgagaaaata gttgaactgg ataaaaagta
      601 ctggacgtgt caaaattatt ttggatcaaa ttagatctat t
//
LOCUS       DAZABD010000197          612 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000197 DAZABD010000000
VERSION     DAZABD010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..612
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.211"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(208..399)
                     /locus_tag="ACHALG_002983"
     CDS             complement(208..399)
                     /locus_tag="ACHALG_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2588266.1"
                     /translation="MKQKFFIAIIATKKFHRGKRFGQKWKFPPKVTAPQLKSIKELEF
                     ENALVICMTCTDEVFRKHF"
BASE COUNT          218 a          107 c           75 g          212 t
ORIGIN      
        1 aattcgcttg tggcgaaacc cgacctggag tttgtctctg tattttgaac cctaaaaatt
       61 aatcaaacag actgatattc tcataatcag cgcgaaataa gccattaagt acagttttcc
      121 tacctatttt tgataacccg ctggttagtt aatattcctt ttccgagaga gataaatgat
      181 tggttaaatc tatatacatc tcaaccttta aaaatgtttt cgaaatactt catcagtaca
      241 tgtcatacaa attaccagtg cattctcaaa ttctagttct ttaattgatt tcagttgagg
      301 agcagttact tttggcggaa atttccattt ttgtccaaac ctcttccccc gatgaaattt
      361 ctttgttgca attattgcaa tgaaaaattt ttgtttcaaa aatatacata attacttcct
      421 ccaataaaaa ctatctacct ctcaaattat ttttctctaa taagaaaaac agaaaaggta
      481 ataagataca acagtgatga ttcctaataa agaaaagact acaataatat ttttctaact
      541 tatcgccttc aaagaaatag tttcttattt ttcactaaaa taaaaaattt attgcaaaaa
      601 aaatagaagt ga
//
LOCUS       DAZABD010000198          578 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000198 DAZABD010000000
VERSION     DAZABD010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.212"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>578)
                     /locus_tag="ACHALG_002984"
     CDS             complement(<1..>578)
                     /locus_tag="ACHALG_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFN2588267.1"
                     /translation="IYFVFIFLLVKWIAPFLLSFAEKVYANSSIIIVSLVICLGMSYV
                     ADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFVSVGLEVDFSDFS
                     EQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRGEMALIILQIGQQ
                     SQLIEPQYYSPLVIVILLSTLISPLVLKYFTK"
BASE COUNT          229 a          112 c           89 g          148 t
ORIGIN      
        1 ttttttgtaa aatatttgag aacaagtgga gaaataagtg tacttaataa aatcacgata
       61 actaaaggtg aatagtactg tggttcgatc aattgacttt gctgtccaat ttgtaaaatg
      121 atcaaagcca tttctccacg cgaaatcata ccagcaccga ccatcaatga actatgttga
      181 gaaaaaccgg caatctgagc accaaaataa cctccaagca atttggtcaa aatcgctaca
      241 agcgtcaata tagaaataaa gagtagttgt tcactaaagt cagaaaaatc aacttctaat
      301 cctacactga caaaaaatac tggtataaat actgcatatc ctaaagcctc tacattgtgg
      361 tatacttctt ctttaacctt tgtctgactg acagctatcc cagcaaaaaa agcaccaatg
      421 acagagctaa gcccaacaag atccgctacg taagacattc ctaaacaaat aactagcgat
      481 acgatgataa tagaagaatt tgcgtacact ttttctgcaa atgaaagtag gaaaggcgca
      541 atccatttga ctaataagaa aataaaaaca aaataaat
//
LOCUS       DAZABD010000199          572 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000199 DAZABD010000000
VERSION     DAZABD010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.213"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>572
                     /locus_tag="ACHALG_002985"
     CDS             <1..>572
                     /locus_tag="ACHALG_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFN2588268.1"
                     /translation="APYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLK
                     GNSHSQRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNL
                     RQGYQLYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYS
                     YSNGPLECLNNHIKVLKRNAYGFRNFYNFK"
BASE COUNT          191 a          119 c          109 g          153 t
ORIGIN      
        1 atgcgcctta tgtttcgttg gtgaaaaagc ttttcccgaa tgctcagttg atcatcgatc
       61 gtttccacat cgtccaacat attggcagaa ctttcttgaa tcatcgagtg aaagaaacca
      121 cggtacgact taaaggaaat tcccactctc aacgagggtt agggaaaaaa ttgaagcgct
      181 attggaaact cctacaaaaa gaagaagcaa agttaaacta tgaaaaacgg gtttggcgtg
      241 ccagttttaa agattatttg actgaaagtg agattgccga tcgactgtta gctgcttgtc
      301 ccaacttacg ccaaggctat cagttatacc aagacattct ttatgcggta aagaaaagag
      361 atcagtcact atttgaggat tgtttaacta gagacattag cggtctgcca gaaacctata
      421 caaccacttt acgaacgttt aaaaagtttc tgccacagat ccaaaatgcg cttcactaca
      481 gctattcaaa cggaccattg gaatgcttaa acaaccacat caaagtatta aaacgcaatg
      541 cgtacggctt tcgcaacttt tacaatttca aa
//
LOCUS       DAZABD010000200          567 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000200 DAZABD010000000
VERSION     DAZABD010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..567
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.214"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            71..>567
                     /locus_tag="ACHALG_002986"
     CDS             71..>567
                     /locus_tag="ACHALG_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HFN2588269.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDAT"
BASE COUNT          211 a          104 c           93 g          159 t
ORIGIN      
        1 tacaaaaaag gaagtccctt ctgtagaata aagttaacga caaccaattc acagaaaaga
       61 ggacttccct atgaatgatt ttactacaga aattgtgcaa actctagtca ctaaaggcga
      121 tttaaatgaa ttattccgtt cgcacttaga aaaagcgata aacacactcc tacggactga
      181 attaacggct tttttagatt acgaaaaata tgatcgcact ggttttaatt caggtaattc
      241 gagaaacggt tcttactttc gatcaatcaa aaccgaatat ggtgaattaa cattggaaat
      301 acctagagat cgtaatggtg agtttaaaca acaaacttta ccagcctaca aaagaacaaa
      361 cgatacattg gaaaccacta ttatccattt attcgaaaaa ggtgttacga tgtctgaaat
      421 tgctgatttg atcgaaaaaa tgtacggtca tcactatact ccacaaacca tgtccaacat
      481 gactaaagtt ctgactgaag aagtaaatgc ctttaaatcc agagccttaa atgataagta
      541 tgtcgctatt tttatggacg ctactta
//
LOCUS       DAZABD010000201          543 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000201 DAZABD010000000
VERSION     DAZABD010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..543
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.215"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            22..492
                     /locus_tag="ACHALG_002987"
     CDS             22..492
                     /locus_tag="ACHALG_002987"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HFN2588270.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKITAAIESVL"
BASE COUNT          199 a           99 c           80 g          165 t
ORIGIN      
        1 aaatagaaag gagaaaactt tatgtctatt tataattttg aaactacttt agaagacggc
       61 actgtctatt cattgaaaaa atatgcaggc aaaccgataa tcattgttaa cactgcaaca
      121 aaatgcggtc tagcacctca atttaaacaa ttagaagaac tttaccaagc ttatcagaca
      181 caaggtctgg ttatcttagg ctttccttcc aatcaattta aacaagagct agattcagcg
      241 actgaagcag cagaagcttg taaaaccact tatggcgtta ccttccctat gcatcaattg
      301 attgacgtaa atggagaaaa tacagatcct ctttttactt atcttaaaaa tgaagctcct
      361 ggtactgtca ctaaagcaat aaaatggaat tttactaaat ttttgattga tagaaatggc
      421 aaagtaatcg aacgatttgc accacaaact tctccattga aaataaccgc tgcaattgaa
      481 tccgttcttt aacttaataa aaataaaaaa agatgaacta tttctagttc atctttttgc
      541 ctt
//
LOCUS       DAZABD010000202          528 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000202 DAZABD010000000
VERSION     DAZABD010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.216"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(5..90)
                     /locus_tag="ACHALG_002988"
     tRNA            complement(5..90)
                     /locus_tag="ACHALG_002988"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(54..56),aa:Leu,seq:taa)
     gene            complement(107..178)
                     /locus_tag="ACHALG_002989"
     tRNA            complement(107..178)
                     /locus_tag="ACHALG_002989"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(144..146),aa:Gly,seq:gcc)
     gene            complement(190..262)
                     /locus_tag="ACHALG_002990"
     tRNA            complement(190..262)
                     /locus_tag="ACHALG_002990"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(227..229),aa:Thr,seq:tgt)
     gene            complement(280..361)
                     /locus_tag="ACHALG_002991"
     tRNA            complement(280..361)
                     /locus_tag="ACHALG_002991"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(325..327),aa:Leu,seq:tag)
     gene            complement(369..441)
                     /locus_tag="ACHALG_002992"
     tRNA            complement(369..441)
                     /locus_tag="ACHALG_002992"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(406..408),aa:Lys,seq:ttt)
     gene            complement(445..517)
                     /locus_tag="ACHALG_002993"
     tRNA            complement(445..517)
                     /locus_tag="ACHALG_002993"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(482..484),aa:Val,seq:tac)
BASE COUNT          135 a          152 c          128 g          113 t
ORIGIN      
        1 gaaatgccga ggaccggaat cgaaccggta cggtgatcac tcaccgcagg attttaagtc
       61 ctgtgcgtct gccagttccg ccaccccggc gcgattgctt tggcaaagcg gaaaacgggg
      121 ttcgaacccg cgacccccac cttggcaagg tggtgctcta ccactgagct atttccgcgt
      181 gtttaaagat gccggctaaa ggacttgaac cctcgaccct ctgattacaa atcagatgct
      241 ctaccaactg agctaagccg gcatgtaaaa cttaaattat gcgggtgaag ggacttgaac
      301 ccccacgccg taaggcgcta gatcctaaat ctagtgcgtc tgccaattcc gccacacccg
      361 caaaaatatg agccgtacag ggctcgaacc tgtgaccctc tgattaaaag tcagatgctc
      421 taccaactga gctaacggct catatggagg ttaacgggat cgaaccgctg accctctgct
      481 tgtaaggcag atgctctccc agctgagcta aacctccatg gataactt
//
LOCUS       DAZABD010000203          499 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000203 DAZABD010000000
VERSION     DAZABD010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..499
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.217"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          166 a           96 c           58 g          179 t
ORIGIN      
        1 ataagaatgc taaatcactc tacaataacg gcgaaagcac aaaaaataaa aaaattatta
       61 cgttattaaa aaaacagatg aaagatcagg ttttattaga tctcattctt tcatcacttg
      121 aagggattta atgaactact ttttcctctt tgtcgttttt atatattcgt attccttcaa
      181 acagtaggat agcctctttc aatgcttgat atttgaactg tccgttactt aaaaatttag
      241 atgtgccgcc ctcttcctag aacatattct tttttacttt ttcattaatt gacagaccaa
      301 tcgaacgaaa ctgtaaattt cgataggata atgattggtc aaactcattc attttcaaga
      361 catctcgatt catattcaag ataggaatct ttttatcaac atcactttat tcgtattttc
      421 ctctaccata aaagcttcac gctccacaaa attgttttat acactactat tcagcccatt
      481 caacgaccaa tgaattttt
//
LOCUS       DAZABD010000204          468 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000204 DAZABD010000000
VERSION     DAZABD010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.denovo.219"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>468
                     /locus_tag="ACHALG_002994"
     CDS             <1..>468
                     /locus_tag="ACHALG_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HFN2588271.1"
                     /translation="CVIWGIINGYTSQRATYRAVCSVLFPNGDFPSRSRFTRLSSNLA
                     YTIKIIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYF
                     YGLKIHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHK"
BASE COUNT          155 a           86 c           83 g          144 t
ORIGIN      
        1 cttgtgttat atggggcata attaatggct atactagcca aagagccacg tatagagcgg
       61 tttgttccgt cttatttcct aatggtgact ttcctagtag gagtcgattc actcgcttaa
      121 gttcaaactt agcttacact atcaagatta ttcgatactt tttcatcaag aagctcacca
      181 aaggtgaact agtcggaatt atagatagtt ttcctagtcc attatgtaaa ccagttagaa
      241 atagacaagc aaaactattg aatcaaatag ccaaagttgg ctataactca acaaaaaaat
      301 cttattttta tggtcttaaa attcatatga tcgttactaa gactggcttt ccgattactt
      361 actcaatcac gaatccaggt gtccatgacg tgaaagtgtt agagacttta tcggaagaag
      421 cgaatcttcc caatatccta ggggacaaag gatatattag ccataaaa
//
LOCUS       DAZABD010000205        12801 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.guided.074, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000205 DAZABD010000000
VERSION     DAZABD010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12801
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.guided.074"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(101..544)
                     /locus_tag="ACHALG_002995"
     CDS             complement(101..544)
                     /locus_tag="ACHALG_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFN2588272.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINAMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILTSKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(739..2079)
                     /locus_tag="ACHALG_002996"
     CDS             complement(739..2079)
                     /locus_tag="ACHALG_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFN2588273.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGIILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHAADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFAAYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLIIFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLTLRKGTLW
                     DKGISFFALAVLWLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2361..3257
                     /locus_tag="ACHALG_002997"
     CDS             2361..3257
                     /locus_tag="ACHALG_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFN2588274.1"
                     /translation="MNKNDVLLIAKNKSLYFFPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMDFSSSEPKTDLFFGATNNGRKERAEKYKQEFTKR
                     GVTTNYFILPLRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYQFYHSENIFLLQERDLDELPEFLHTPFQPIPSELLEFFDAENWAKR
                     FFTTDSAIYEQYEYDLSSVKNK"
     gene            complement(3400..4341)
                     /locus_tag="ACHALG_002998"
     CDS             complement(3400..4341)
                     /locus_tag="ACHALG_002998"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFN2588275.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVVLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNKHVDLIVALTKANQLDDMFRSIQSIITENTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEAKLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAEGM
                     VRTIVSEFASIAAKEGITLDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAVWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4467..5588
                     /locus_tag="ACHALG_002999"
     CDS             4467..5588
                     /locus_tag="ACHALG_002999"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFN2588276.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALGGTWRTIDGMDLLFRQRKISYLLTNWQGKNTAVIESAQVLGLDLLDKP
                     TDQTIRLASSYALGELIKDTLLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTRKLHLDRRNVLVSKVKVIIAADVTNPYTGQQGAAWQFGRQKGGTDTTLAFLD
                     KQAEKIAQQIKDVWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFELVSQITGLEKQI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDRELEGLFDFLPIV
                     LSIQLGPIDLEEAMEHRQTLEKTEILGQTLSRFFNILPK"
     gene            5846..7348
                     /gene="eat(A)"
                     /locus_tag="ACHALG_003000"
     CDS             5846..7348
                     /gene="eat(A)"
                     /locus_tag="ACHALG_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFN2588277.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQAFVYFPQQTKDKERLTYYVLNEIMDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEIAKNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKKVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDALTMNSQVSHHKRLLSVENLQLGYENLLFEPVHF
                     TIEPHQRMAISGPNGAGKSSIIHYLLGTFNGKVVGEKSQPKHLTISYVRQNYEDNRGT
                     LAEFAKKNRVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYI
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLEKI"
     gene            7415..7942
                     /locus_tag="ACHALG_003001"
     CDS             7415..7942
                     /locus_tag="ACHALG_003001"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFN2588278.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQALAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVVDRLNLPMTADERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECMEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELISYE"
     gene            8154..9215
                     /locus_tag="ACHALG_003002"
     CDS             8154..9215
                     /locus_tag="ACHALG_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFN2588279.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQNRLIELGSEAKKNKLELMVDISGEALKRAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPNDIDELKEAAADFSQIEAWHNYYPRLETGLDKEWYQEK
                     NQWLKTQGFLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSEHVQEQFASFQKEQTVLLHTEPVDEVFYEYILGKHTNRQDDARDVIRSADARF
                     REIPPIPVRNTAKRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            9218..10114
                     /gene="murQ"
                     /locus_tag="ACHALG_003003"
     CDS             9218..10114
                     /gene="murQ"
                     /locus_tag="ACHALG_003003"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFN2588280.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELPSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISEHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEAAQAKLAEANGFVKGTL"
     gene            10128..12109
                     /locus_tag="ACHALG_003004"
                     /pseudo
     CDS             10128..12109
                     /locus_tag="ACHALG_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
     gene            complement(12179..12625)
                     /gene="nrdI"
                     /locus_tag="ACHALG_003005"
     CDS             complement(12179..12625)
                     /gene="nrdI"
                     /locus_tag="ACHALG_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFN2588281.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEYEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQRFGFPFLADYELRGTPSDVTRVYQILAEKNK"
BASE COUNT         4220 a         2332 c         2659 g         3590 t
ORIGIN      
        1 cacaacctct gattcacggt gttccagaag tagcttcttc ttatttcttg aagttgatca
       61 cttctggaac atcctctttt tttatttgga tggataatgg tcattccatt tctttgatca
      121 tttttttcat tttgtcgatt tcttcttctt gtgcctgaat gatttcttcc gctaattttt
      181 ttacttcagg atcttttaaa tcagctcgtt tacttgtcaa gatagcaata gagtgatgag
      241 ggatcatcgc cttcatccac gccgtatcgt tgacagttgt ttggcttcgt accataaata
      301 aggaaccggc aaaaacaaga agactagcgg ctaaaatcat agcatttatc tttttgtttt
      361 catacatttt ccacatgaac aacaacatga tgactgccat gactgcaccc atcatcaacg
      421 ccatgaatac acgtgtgcga ctgaagaaga tatgatcaag cgcataaaca ttcaaataca
      481 tcataccaaa catgactacc gtcgaaacac caatcatcaa caaaaacttc caatactttt
      541 tcatttaaaa caaactcctt tttcttcatt cctttcttct atcatgtcat gagcgtggag
      601 aacaatcaat taatctgcac agatggtttt tgaacaccgt ttattcggaa aaaagaactg
      661 caacaatttt tgtcgcagct cctttttttc aatagctcca gatattcaat ataaatagaa
      721 taaaattctc tattggtttc attcattcgt caagacgaaa gcatcattct ttttttgata
      781 actgaaataa atgaacaggt aaagcaaacc ggtcaataca gttaccccaa tggagttcgt
      841 gaataggata ccaaggaaaa tcaaccataa gactgctaat gcaaaaaagc tgattccttt
      901 gtcccataac gttcctttcc ttagtgtcag tacaaaggac gcagcactga taatgatcca
      961 gatcaataat actgtatagg aaagagaacc tgccagatag ccaaataatt tgtcaccgac
     1021 aaaataagaa agagccacac ctagataaag aacactggta caaaacagca cagcacgttg
     1081 cggtacttga tgcttgttta aaacagctag ttttctagag gaacctttcc gatctttcaa
     1141 gcggaagaac aaaattctcg aagaagcata tacacctgaa ttgatagacg agaacagagc
     1201 aagaacaatc acgaaattga cgatatctgc cgcaaatgga atattcattt tttcaaatac
     1261 catcacaaaa ggactaacgt tcgtgcctgc taaagtattc caaggaaaaa taatcaaaag
     1321 caaaaataaa ggaataatgt aaaaggaaat gattcttccc atgacgcctt tgattgcttt
     1381 aggaatcgca cgtttaggat catctgcttc gcttactgtg atagcaatca actcagaacc
     1441 gccgtaagaa tagataacta caagcaaaga actgatgatt cccttcatac cgtggggagt
     1501 aaaaccacca tgatcagtta attgttgaag ggtagggaaa acaccagtac ccaacatttg
     1561 ctgaccgaca agataagcag caaaaatgat caataggata atgacactga ttttagcaaa
     1621 ggccagccaa tattctgtct ctgcgaataa tcgtaccgaa taaagattga tgatagtagt
     1681 taaaacagcc aacataaaaa caaataccca agctgggatg tgggggaacc acaactgtaa
     1741 aaaggaagca gctgcaacag cttcagcaat gatattgata atccacatgg accagtagac
     1801 ccaatcggta aaatcggcag catgagcacc tagatatgga cgaatcaatc cagataatcc
     1861 gtggtgcgta tcaccaccac ttaaaacaat ttttcccaga cctttcatca cgatatataa
     1921 aattatgccg ccaatcaaaa aagcgatcaa tacagaaggg ccggcgattc caatcgcttc
     1981 gccacttcct ttaaatagac ctgcaccaat cgctccacct aaagctagca tggtgatatg
     2041 tcgggagcta agttgttttt tcaatttttt atcttccata aataacctct ttcttttaaa
     2101 ttcacaatca aaatgcatag taagaataaa tagtaagaat aaatagtaat aataataatg
     2161 actaatgata acaaattttc agataattac aactattcag aaaacttttg ggcatatttt
     2221 aatggaagaa tgatttattt catcgtattt tcatagaaat gttaaatttt tataaaaata
     2281 ataggaagcg ttttactggc tttttactat ttaaacaggc tatcatagga aaaagtgaca
     2341 gagaaagtga ggcggaacag atgaacaaaa acgacgtatt gctaattgcg aaaaataaat
     2401 cactttattt ttttccttcc atccgctctt tagatgtttg gtccggaaaa gatttactac
     2461 agtcggaatt cattaaatca gctcgacaac agttggcaaa ttatcagctg atcgtttttt
     2521 tagattatgg atttgatatg gagatggctc gacgcgtgcg tccatttacc aatgcaaaaa
     2581 tcgtgttatt tttttggaac cactttaaag cagaacacga ggcgatgctg aagcaggcac
     2641 aagatgaacc ggcaatcgat gagatttatc attttgattt tttagaagcg aaagaacttg
     2701 gactaaaaca taatagttcc ttttattcga aacatatgga tttttcttct agcgaaccaa
     2761 agacggatct attttttggt gcaacaaata acggcagaaa agaacgggca gaaaaatata
     2821 agcaggaatt tacaaaaaga ggtgttacga ccaattactt tattcttccg cttcgaggaa
     2881 atgaacaacc tggatacctt acttatgaag aatatctgaa aaaaaccggg gaatcgaggg
     2941 ggattctaga actgttgcgg gaagggcagc aaggagtgac tttgcgaacg tttgaatcta
     3001 tgtattttca aaagaaacta gtgacagata atcaagccat agctcattat cagttttatc
     3061 attctgaaaa tatttttttg cttcaagaac gtgatttaga tgaattaccg gaatttttgc
     3121 atacgccatt tcaaccaatc ccatcagaac tgttggaatt ttttgatgca gaaaattggg
     3181 caaaacgttt tttcactact gactccgcta tttatgaaca atatgaatac gatctttcct
     3241 ctgttaaaaa taaataaaag tgccaaccaa tcatagatga aaataaacaa gcgcaattct
     3301 tatggcaaca tgagaattgt gtttttcttt ttgaactttc atgggggtaa aaaaatgaga
     3361 accagctgct gatccagctg attctcatca gagagccttt tattctgcgc caaggattcc
     3421 ttcttttgca tggactaatt gggtcaaaaa ggcacaatat ggtgtagcaa tatcatattt
     3481 ttgtcctttt cgccaaacgg caccattgat gtagtcgatt tcagtcaaac gatggttttt
     3541 gatcaggtct tggtacatag atgggtaatg caacccgatg ttgtcaggat cataagtaga
     3601 agcaatatgt tggtacactt cttcttgatc taatgtgatt ccttcttttg ctgcgataga
     3661 agcaaactca gatacaatcg ttcgaaccat gccctctgcc gctttttgcg cacctaattc
     3721 agcaatatta caatccaata tcgtgcataa tccatttaat gttccgttta cacaagcttt
     3781 tcgccaaatg gaataacgaa cattatgact atagattgga ttcaattttg cttcttgaaa
     3841 gacttctaca actttttttg taaatgcttc gccttctggt tccaagttct ctagttcgat
     3901 ttctccatca cctaataatg tgacttttcc gggaccagct agaccagctg tccacatcgt
     3961 gatcccaaat aagatatttt tctttggtac atatttttcc agtacatctt catgacctaa
     4021 tccattcagt aaacacaaaa cataagtatt ctcagtaata atggattgga tcgaacgaaa
     4081 catatcatct aattgattcg cttttgtcaa ggctacgatc aaatccacgt gtttgttgct
     4141 ttcgatgatt tcttctggtg aatagattgg cagttttgca actacttctt tgccattaaa
     4201 gtcagcaatc aatccatttg tacgaatggc ttcaatgtga gcaggccagc gatcgatcaa
     4261 gacgacctca tttccgcttt gatgcaacat taatccgatt cggctgccca ttgctccggc
     4321 tccagctatt gcgattttca tgttttttct ctcctttatt caatgagttc ccttttagta
     4381 tatagaaaaa cagaagaaaa tagcattgtt ttgtttgaaa aaagtgaaaa aaagtgtctt
     4441 acttaagaag aaagtgaggg atatagatgg atattttggc ggcaatcgat tcatttaaag
     4501 gaagtgcttc ttcatttgaa ttaaatcagg cagttctaaa tggttgggaa gaccgtaaag
     4561 gaagaaaaat caatgtgccg attgcagatg gcggagaaaa tactgcagaa gcaatctatt
     4621 acgcgctcgg gggaacctgg cgtacgattg atgggatgga tctcttattt cgtcagcgaa
     4681 aaatcagtta tttattgacg aactggcaag gaaaaaatac cgcagtcatt gaatctgcac
     4741 aagtattagg attagattta cttgataaac caacggatca aaccatccga cttgcttcta
     4801 gttatgcctt gggagagctt atcaaagata cactgcttca agaagtggaa caaatcattg
     4861 tcactttagg aggaagcgga tgtagcgatg gtggtctagg attactccaa tctttgggtg
     4921 cttctttaga agggtacaca gaaggaaatc ctttgttatc tactagaaaa cttcatttgg
     4981 atcgaaggaa cgttcttgtt tctaaagtaa aagtgatcat cgctgcagac gtgaccaacc
     5041 catatacagg tcaacaagga gctgcttggc aatttggaag acaaaaagga ggaacggata
     5101 cgacgttagc tttcttggat aaacaagctg aaaagattgc tcagcagata aaagatgttt
     5161 gggaggtgga tttagatgac atcccaggaa gcggagcagc aggcggtatt ggcggagccc
     5221 tttttttgtt aggagcgaag atggtctctg gttttgagtt ggtcagccaa atcactggat
     5281 tagaaaaaca gatcgaacaa gcggatgtca tcattacagg agaagggcgg attgatcagc
     5341 aaacaatcca cggaaaagtt ccatatggcg tagcagtgct tgcaaaaaaa tatcgaaaac
     5401 caataatcgc tatttgcgga agtagagacc gagagctgga aggactcttt gattttctcc
     5461 ccattgtctt aagcattcaa ttaggaccaa tcgatttaga ggaagcaatg gaacacaggc
     5521 aaacattaga aaaaacagaa atcttaggac agacactcag cagatttttc aacattctcc
     5581 cgaagtagtt gacaaaaaca atttctttat gctatcgtaa tttcaaatat aggacaaagt
     5641 gagggatccg tgcattgaaa aattaagtcg attgatgaaa gctgcattca gactgtacct
     5701 tgtggagaca agggagcagt gtgcagattt tcgggattgg cttggttctt tcgatgtatg
     5761 ggtctttttg tgtgccccaa cccttgcatc ctctttagcc agttctgaaa ataggactgg
     5821 tcttttttta cgaggtgatg gaataatgtc taaaatcgaa ataaaaaatc tgacattcgg
     5881 ctatgacagc caaggcacat tattatttga acaagccaat ctaaactttg acacacaatg
     5941 gaaactagga cttatcggac gaaacggtcg aggaaagaca actttactga atatcctgca
     6001 aaacaaactc ccttatcaag ggcaagtgat ccatcagcaa gcgtttgtct atttcccgca
     6061 gcaaacaaaa gataaagagc gtttgaccta ttatgtgtta aatgagatta tggattttga
     6121 gatatgggaa attgaaagag agctccaact gatgcaaaca gatcctgaaa tcttatggag
     6181 agaattcagc acactatcag gaggagagaa gacaaaagtc ctactggcac tcttatttgt
     6241 ggatgacact catttcccgt taatcgatga accaacgaat catttggata tctctggcag
     6301 aaaacaagta gcggcttact tgaaaaagaa aaaacaagga ttcatcgtgg tgagtcatga
     6361 tcggggattt atcgatgaag tagtggacca tgttttagca atcgaaaaga gccaactgga
     6421 actttatcaa gggaatttct ctatctatga agaacagaaa aaacttcgtg atgaatttga
     6481 aatagctaaa aatgaaaaat tgaaaaaaga agtcagcagg ctaaagaaaa cagcagctga
     6541 aaaagcagaa tggtctcgtt cccgagaagg ggacaaaaca aagaaaaaag tcggattcat
     6601 cgatacggaa tctaggcgag tgaataaagg agcagtaggc gctgatgctg cacggacgat
     6661 gaaacgatct aaagcaatcg tgaatcggat ggaaacccag atcagtgaga aagaaaaatt
     6721 attaaaagat atcgagtata tcgatgcttt gacgatgaat agccaagtgt ctcatcataa
     6781 acggctgtta agcgtagaaa atcttcaatt agggtatgaa aatctgttat tcgagccagt
     6841 tcattttacg atcgaacctc atcagcggat ggcaatttca ggtcctaacg gcgcgggaaa
     6901 gtcatccatt atccattatc ttctggggac attcaacggc aaggttgtag gagaaaaatc
     6961 ccagcccaaa catttgacca ttagttacgt acgccaaaat tatgaagaca atcgagggac
     7021 attggcggaa ttcgcgaaga aaaaccgagt agactatcaa gcatttttga ataatctccg
     7081 aaagcttggg atggaaagag atgtttttca taataaaatc gaacagatga gtatgggaca
     7141 acggaaaaaa gtggaattgg ctaaatcttt atcccaacca gctgagctct atatatggga
     7201 tgaaccattg aattatttgg atgtcttcaa tcaagaacaa ttagaacaac tgattttgaa
     7261 cgtgaaacct gctatgttac tagtggaaca tgatcaaacc tttctagata aagtatctac
     7321 tgagattatt tctcttgaga aaatctaaac cttgtaatct caaagacaac aaggtatggt
     7381 taaggagaac tttgtcaacg aaggagagaa tcacatgctt tatgtaacga gacacggaga
     7441 aacaacttgg aacgcacaag gattagtttg cgggcgagca gatgtgccgc tgactgaaaa
     7501 aggacagatg caggcacagg cactagcaga aaaggtagta gatttgcctg ttccaatcac
     7561 gaaaatcatc cattcccctc ttcaacgagc aagagataca gcacaagcag tagttgatcg
     7621 tctcaatttg ccgatgaccg ctgatgagcg attggtagaa atggattttg gggattatga
     7681 cggactgcca agcaaagatg aaaactttca aaaagcgcgt ttagcgtttg ctgtacgttt
     7741 cccaaatgga gagtcggtct tagatgtgta cgcacggatc gttcctttat tgaaagaatg
     7801 catggaagac gaagaaaatg tctacttgtt agtctgtcat aatgccttga taagagtgat
     7861 caatgcttat tttcatccaa tgcctaatga agggttcttt acattcatgg tagacaatac
     7921 agaattaatt tcttatgagt aattagactt ttgtgttatt taaataaaat gtttcaaggc
     7981 actgcttttg cttagcagtg ccttttttgt ggctgtgtca attcttttgg cattataaat
     8041 ccaattttta gaaataaaat ttaattattt taacaaaagt attgaaattt tgtttcttta
     8101 gatatatact ctagtcatag aaatgaagac ggatacagaa aaagagggat aatatgtttg
     8161 gcttttcaat atttatgaat gattctttgt cagaagagaa gaaacagtat atccgccaaa
     8221 tggcatccaa tggctttgtc ggtattttca cttccatgca tataccggaa gatgatgttt
     8281 cggcttatca aaatcgattg atcgaactag gaagcgaagc gaaaaagaat aaactagagt
     8341 tgatggtcga tatttcagga gaggcattga aacgagctgg cttttcatta gaagatctaa
     8401 cacccttgaa agcaattggt gtaacagggt taagaatgga ttatcatatt tctaatcagc
     8461 agatcgccga ctggtcacat caattgaaaa tcagtttgaa tgccagcacg atcacgccta
     8521 atgatatcga tgagttgaaa gaagcagcgg ctgatttctc acagattgaa gcttggcata
     8581 attattatcc acgtcttgaa acagggttgg acaaagaatg gtatcaggaa aaaaatcaat
     8641 ggctcaaaac tcaaggattc cttgtacaag gctttgtacc aggagataca gagttgagag
     8701 gtccactgta tcaaggatta ccaactttgg aagaacatcg agggatccat cctctggcag
     8761 gtgctcttga tctattagca agtaacacgg atattgttta tatcggagat gcaggtctgt
     8821 cagaacacgt tcaggagcaa tttgcttctt tccaaaaaga acagacagtc ttgctccata
     8881 cggaacctgt agatgaagtt ttttacgaat atatattagg aaaacatacg aatcggcaag
     8941 atgatgcaag agatgtcata cgaagcgcag atgcgcgttt tcgagagatt ccgccaatcc
     9001 cagtaagaaa tacagctaag cgtatgaaag gatctattac tttagataac gaaaagtatt
     9061 tgcgctatat gggtgagatc caattaacta aatatgattt gccagcggat gaaaaagtga
     9121 atgtagtagc caaagtcatc aaagaagaac taccgctgat caaccaaatc aaagctggta
     9181 tgaactatca atttataaga aaagaaggaa gataaaaatg agcaaaatca atttagagaa
     9241 cctaacaacg gaacgcagaa accaagaaac ttttggatta gatgaaatga gtatttctca
     9301 agcagtccaa ctcatgaaca aagaagatca gaaagtggcc gctgctgttg aaaaagaact
     9361 gccaagtatc gagccagtga tcgccaacac gattgaatcc tttaaaaaag gtggtcgctt
     9421 gatttatatg ggagctggaa cgagtggtag acttggtgta ttagatgctg ctgaatgtgt
     9481 accgacattc ggtgtagaac cggaaatggt cataggccta attgctggtg gagaagaagc
     9541 aatgactgta gccgtcgaag gagccgaaga ttcttatgaa ttagggcaag aagatctaat
     9601 caagctgaat ttaacaaaaa atgatatagt tattggtatc gcagctagcg gtcgtacacc
     9661 atatgtgatt ggtggattag attacgcaaa atcgattggt gctgttacag gaacgatttc
     9721 ttgcaataaa caagcagaaa tcagcgagca tgcggatttc ccaatcgaag tcgatgcagg
     9781 accagaattt ttgacaggct ctactcgatt gaagtcagga acagcacaaa aactgatttt
     9841 gaatatgatt tctactatca gtatgatcgg tatcggtaaa gtattcaata acttgatggt
     9901 cgacgtcaaa ccaacaaatg aaaaactaat tgaacgctca aaacgaatca tcatgcaagc
     9961 gacagaagct gactacgaaa cagctgaaaa gtactttgag gaagcagatc aaaatgtcaa
    10021 actggctatc gtcatgattt tgacaaatag tgataaagag gcagctcaag caaaattagc
    10081 tgaagcaaac ggttttgtaa aaggaacctt atgaggggga aaagaccatg gcaaaaaacg
    10141 aattgacttt ggaagaactg gcaaaacaaa tttatcaagg agcaggcggt atgggcaatg
    10201 ttgaaagcat tgtccactgc atgactcgtg tacgtatgaa ggtcatcaat gatgatcttg
    10261 taaatgtctc ggaattaaaa gcgattccag gagtacttgg cgtagtagag gatgaacagc
    10321 tccaagtcat catcggacct gggaaagtga acaaagtagc gcaggaaatg gttgacttag
    10381 caggcgtgaa attaggagaa aaacttcctt ctgctagtgc aactacagca agttcttctg
    10441 catctggaaa agaccgtgta aacgaacggg cacaagaaat gaaagcggca caaaaatcga
    10501 aacaaaaacc ctctaaaatc aaatcggtat taaaagacat ctctaatatc tttgtaccaa
    10561 tgatccctgc atttgtcggc actgggatcg tggcagggat tgccgcagtt ttagcaaact
    10621 tagtcacagc tggaacacta gacgcaggaa catggcaaca atatatcgat gtgatgaaca
    10681 tcttgaaaaa cgggatgttt agctatctag ttatctatac aggtatcaat gctgcacaag
    10741 tttttggtgc aacgccaacg cttggtggag ttatcggggc tgttgtgatg ctgacaggga
    10801 tgaatccaga agcaccactg aaaaacttgt ttaccggtga agctcttgca actggacaag
    10861 gcgggatctt aggtgttatc ttcgcagttt ggttattgtc aatcgttgag aaaaaactgc
    10921 ataaagtagt acctgatgcg gtagatatca tcgttacgcc aactctttcc ctgattgcca
    10981 tcggtctgat cgaaattttc ttcatcatgc ctttagcagg gttcatttct gacggcatgg
    11041 tcggtgggat caactgggta cttggtgttg gaggagcatt tgccggcttc gttctaggta
    11101 cattattctt gccaatggtc atgttcggac tacatcagat cttgacgcca atccatgtac
    11161 aaatgattga tgaaactggt cgtaccttat tgcttccaat ccttgcgatg gccggagcag
    11221 gacaagtggg tgcagcattg gcattatgga tccgctgcaa aaaagacaaa gaattaacag
    11281 aaatgatcaa aggggcttta ccagtcgaat cttaggaatc ggcgaaccat tgatttacgg
    11341 agtaacatta cctctaggac gtccatttat tactgcttgt atcggtggag gtatcggtgg
    11401 ggcagtgatc ggaatgatcg gaaatgtcgg agcaatcgct attggacctt caggagccgc
    11461 tttgattcca ttgatctctg atggtaaatg gttaggctat atccttggtt tattggctgc
    11521 ttacgctggt ggcttcgttg ctacattctt cttcggtatt cctaaagacc gtttagaaaa
    11581 agaagaacta gcgaccgaaa cagccacagt aaatgaagta cctgaaaaag cagtacctca
    11641 agcttcagct gaaagtgtga ctatcttatc atcagttgct gacggaacag ttgtttcttt
    11701 agataaagca agtgacccag tttttgcaca aaaaatgatg ggtgaaggtt actttgtaga
    11761 accagaaaac ggacatatct attccccggt tactggagta ataagttctg ttttcccaac
    11821 aaaacatgct atcggcatta cgacaccaaa tggcttagaa atcttgcttc acatgggcat
    11881 caacacagtt gacttaggcg gtacgccatt cgatgtgaaa gtaacagaag gacagcaagt
    11941 gacacctgat acgcttgtag cagaagctga cttagaagca atcaaagcag cagacaaaga
    12001 aacctccatg atcgtcttgg taaccaatat ggatcgtgta aaaaactttg tcttggaaaa
    12061 aacaggtaaa gcgaaagcaa aagcacctgt gatggatgtc aaagcttaat aagaatagaa
    12121 acaatagaga acataaaaag aagaagccga aatttatttt tcagcttctt cttttgtgtt
    12181 atttattttt ttctgccaaa atttgataaa cgcgtgtcac atcagatggt gttccccgta
    12241 attcataatc tgccaagaaa ggaaagccga atctttgtgc gtattgtttg gctgtcagac
    12301 aatattgatt gttgaagttt ttattaccgc taccgactac accaaggcaa agtttatgat
    12361 tgtcttcgta ttccagatat tcgcgcatcg tttcggtcaa gatttctgtg tcaccgttgt
    12421 ctaacccatt cccgccgtct aaataagtag gaacgaaagt aaaaaatggt tctgtttctt
    12481 tcgtaaaatc actattttca tggatttctt ttaaagtaac ttcaggattg acaggatctt
    12541 ctgcatgcat tttttcagca tattctgcta gatgtttggc aaaagcacgc gtatttcctg
    12601 aaatcgatat atataaaatg ttcatggatc atcccccaat tctttccctt tagtatagca
    12661 tctttttaag tttttttggt ggtgtaattt ggaaagggac taggcgcttt gcttgtataa
    12721 tgagaaaaaa gtcggtagaa ttatatttct attaaagttt ttattaatta agtaaaaata
    12781 aaaaaggttt gttatacttt t
//
LOCUS       DAZABD010000206         9872 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.guided.089, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000206 DAZABD010000000
VERSION     DAZABD010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9872
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.guided.089"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..872)
                     /locus_tag="ACHALG_003006"
     CDS             complement(<1..872)
                     /locus_tag="ACHALG_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFN2588282.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            1000..1569
                     /locus_tag="ACHALG_003007"
     CDS             1000..1569
                     /locus_tag="ACHALG_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFN2588283.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            1636..2373
                     /locus_tag="ACHALG_003008"
     CDS             1636..2373
                     /locus_tag="ACHALG_003008"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFN2588284.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            2604..3872
                     /locus_tag="ACHALG_003009"
     CDS             2604..3872
                     /locus_tag="ACHALG_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFN2588285.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            4359..4823
                     /locus_tag="ACHALG_003010"
     CDS             4359..4823
                     /locus_tag="ACHALG_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFN2588286.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVCVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            4807..5436
                     /locus_tag="ACHALG_003011"
     CDS             4807..5436
                     /locus_tag="ACHALG_003011"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFN2588287.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            5790..7268
                     /gene="msr(C)"
                     /locus_tag="ACHALG_003012"
     CDS             5790..7268
                     /gene="msr(C)"
                     /locus_tag="ACHALG_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFN2588288.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            7315..7761
                     /locus_tag="ACHALG_003013"
     CDS             7315..7761
                     /locus_tag="ACHALG_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFN2588289.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(7960..8475)
                     /locus_tag="ACHALG_003014"
     CDS             complement(7960..8475)
                     /locus_tag="ACHALG_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFN2588290.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            8592..9793
                     /locus_tag="ACHALG_003015"
                     /pseudo
     CDS             8592..9793
                     /locus_tag="ACHALG_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT         3134 a         1743 c         2058 g         2937 t
ORIGIN      
        1 taactgtatt tctccgttag gacatctaaa aatttacgag cagcaggaga aagaggctgg
       61 tcttttttcc aaatgagatt cagtgaagct tgtaatggcg gatcgagtgg gatgaatatt
      121 ttttccgtcc cataagtatt gatgatccca tctagagcta atgcaccaaa agtcccgtta
      181 tcgaccatca aagaagcatt gtacaataag ttatatgttc cgacgatcga taactgatct
      241 atgctgaaac ctagccattc agctagttga ctgtccactg acgtttgccg ggaaatgatc
      301 aaaggaacag ttttcagttc ctgcggatgg atgacggttt gctttgctaa aggatgattt
      361 cgatgtatca aaagtcccca acgatcttgg gctggcagtc gaaatgactg gtatttttgt
      421 ttgtttgtcg gttcgattac gagtccaaaa tcaagtaatc cgctatccag tttgtctatg
      481 atatcatctg cattcccgct atataaatga aactggatat caggatgcgc cttgatcaac
      541 gaatgaaggc tatcagaaat gaaagagatt gctttagttt cgcctccgcc aatgtagatt
      601 tctccagaga tgattgcgtc atttgttaaa ttgttcgccg ttttttctac caacgacaag
      661 atttcttttc cttttgaata aagatatcga ccagaatccg tcaactgtat ctctcgggag
      721 ccccgagtga aaagtgtcac accaagttct tcttctaaat ctttcagttg ttttgagaga
      781 gtaggttgcg tgatatgcag agattttgct gctcggctga tatttttttc tctagctact
      841 gctaaaaaat acgtcagtat acgaatatcc attttttccc tcctgttttt aaaaaagcat
      901 accactaagt atagttaaaa ggaatagtta agtatgtttt ataagtatta gacatgataa
      961 gccgtccgtt ataaagtaag gataaaagga gggagcaaaa tgacaagcct catcgaaaaa
     1021 caaatcgtca ataaagagat tcgagtaacg gatccgctct ttgaagtgat ccatcagatc
     1081 caagctgaaa acgaagagcc tttagcagcg ctgaatactg ggtaccatga accagcagat
     1141 atccgcaaac ggctagaaaa tatcattagt gacaaagtag atgatacggt gactgttctg
     1201 ctgccttttt atacagattt cggcaaacat attagtatag ggaaaaatgt ctttatcaat
     1261 cgtcaagtga tgttcgttga cttaggtggc atctgtttag aagattcagt attgattggt
     1321 cctcgtgtca acctcatcac ggtgaaccat ctaaccgatc caaaagaacg aagaggcttg
     1381 tcagtaaagc caatccacat caagaaaaat gcttggatcg gagcaggagc aactattctg
     1441 ccaggcgtaa cgattggcga gaatgccatt gttgcagcga atgcaactgt tacgaaagat
     1501 gtcccagaca ataccatcgt tgcaggaatc cctgccaaaa tcgtcaaacc tgtcgaacgg
     1561 attatttgaa cagtaagatg aaataagaga tagtgagtat aagcaagaga tttttcaaaa
     1621 caggaggaga atagaatgaa tgatttatca gaaaaagtga ttgtcatcat gggcgcttca
     1681 agcggaatcg gtgaagcaac aacgaagcta ttagcagaaa aaggggctaa actagttata
     1741 gctgcacgcc gagaagatcg cctaaaagca ataaaagaat ctttaccaga agctgagcta
     1801 tatattcaaa cagcagatgt aagagatttt gcacaagtcc aagcagttat cgatttagcg
     1861 atggaaaagt tcggtcgaat cgatgtattg tacaataatg caggaatcat gccaaccgct
     1921 ccattagttg aaggtcaccg tgatgaatgg caaaatatgc ttgatatcaa catcatgggt
     1981 gtgttaaatg gaatttccgc cgtacttcca atcatggaaa aacaaaaatc tgggcatatc
     2041 atttcaactg attctgtggc gggacatgtt gtttatcctg attccgccgt ttattgcgga
     2101 acaaaatttg ctgtccgtgc aatcatggaa ggattacgtc aagagcaacg gcaaaataac
     2161 atcaaatcta cgatcatctc tccaggagct gttcaaactg aattatatca aaccatcagc
     2221 gacaaaaagg ctgctcttga acttcatgaa gcgcaaaaag aatggggatt gacctccgaa
     2281 gatatagctt ctgctgtagc atttgcgata gaaacgccag accggatgtc tgtcagtaac
     2341 atgattatcc gtccgacgac acaggaagta taatccataa cggataatat ccaaagagaa
     2401 ttacttgtta aatagttatt caaacgacca tcaatttaag gcgtgaatgg agaagaatat
     2461 tcttcattta cgtcttttta tgtagaaaaa gcaaatttat caaaaagaaa caaatgttat
     2521 ttaaatatgg acattgactt gcttttactt ttttagttac actgttattt aggaaaagtt
     2581 tcataattca aaggagaaaa aagatgaaag caaagaattt attatttagc gttattcttt
     2641 taggaaatct agcagttgcc aacacaacag tgctagctga agaatcgaca gaaactgtcg
     2701 caactcagac atcgagttct tcagaaacag gaattgaaaa cgatacagag agtactttgc
     2761 agtcagctac tgtctcagaa aatacaattt caaccgaaga accaacaact gaaacaacag
     2821 aacaaacaca aaccgaagac agtacatcag cagaagctca gaacaatcca gagaatgcag
     2881 aagaaatcac gaaccaattg acagatgggc aaacattgac acatacccat caagcagatt
     2941 tgtctgaatt cgaaaactat actggctatg ctttttatgc tcgctcatct gctaattctc
     3001 aacaagcgtt cattgatagt attgcctcaa ctgcacaaag tttggcaagt gccaatgact
     3061 tatatgcatc agtcatgatt gctcaagcca tcgttgaaag tggctggggg aacagtactt
     3121 tggcttctgc ccctaattac aatctatttg ggattaaagg aagctataat ggccaatctg
     3181 ttacgatgcc tacttcggaa tatgtgaatg ggcaatggat tacggtgaat gccgctttta
     3241 gaaaatatcc ttcgtacaaa gaatcattgc aagataacgt aacggtattg aagacgactt
     3301 cttttcagcc aggcgtttac tactattccg gtgcatggaa aagcaatacg aactcttaca
     3361 aagatgctac ggcttggcta acaggtcgtt atgccactgc gcctaattat ggttctacat
     3421 tgaacaatgt aatcgaaacc tataatctga cacagtatga tacagcacca acttcaactt
     3481 ctccgatgta tcgtctgtat aaccgtcata caggagaaca tttatataca ttgaatgccg
     3541 gagaaaaaaa ttatctgcca actgtaggct gggaatatga agggattgct tggcaagcac
     3601 caaacagcgg acaacctgtc taccgtttat acaatcctta cagtggagac catcattaca
     3661 caatggcaca atctgagatc aactctttaa caaaaatcgg ttggcgttat gaaggtctaa
     3721 gtttttattc tggtggttcc aagccaattt atcgactatt taatccaaat gaaaaaactg
     3781 gtacccatca ctataccttg agtgctaagg aaagagattg gctaacaccg atgggctgga
     3841 gatatgaagg aataggattt tatggttatt aaaaagaggt agtgaaaaag ctgccctgca
     3901 tcaagtagta agaacagcca aacaaaaaca gattttcatg ttttttgttt ggttgaactt
     3961 attaccgcag atgcaagttt ttgaacaccg tttactcaaa aaaggttgtg acaacatctt
     4021 gtcataactt ttttattact gtttaagcat actgatccaa tttctaatat tcgacgggat
     4081 tcttgcaaaa ggcgatttcg gaaatttttg gcggtgtaaa attttctact gcagaagagc
     4141 tatcttctat agtttgataa gaatcaataa tcttggtttt agcgttgtgc tttctttatc
     4201 attttttccg ttcattctac aaaaaatttc ttttctctat acttttctat tgagagcgtt
     4261 ttttttatta cacagctgat tctttgactt ttttcttttt ttattctatt attgacaagt
     4321 caatcgttga cgagtcaatt attagaaagg aggaaattat gtctttagaa gagattagtc
     4381 accttttata tcaaatcaaa ttagccgatc aaaaaatcgt ccagatcttt gaagaaaata
     4441 tcggtataag tcttactaga tacgagatca tgatgagact gaaagaagaa aatccattgc
     4501 tacaaagcag tttgcaagaa aagttgcaga ttgatcaagg agcagttact cgtcatctga
     4561 aggtattgga agcaaatggc tatgtttcgc gtcagagaaa tcctcagaac aaccgggaag
     4621 tgtgtgtagc gctaacagaa aaagcccaga agaaaatcga gcattgtgaa atggaccata
     4681 aagatacagg ttccgtgcta tccccggctt tcagcgatgt agaaatgaaa caatttctaa
     4741 cacttttaga taaattgaat acccaacttt gcaatataac aacagaaacg aaagaggtta
     4801 ataataatgg aacaaaaaaa tgatttttca acaattatga ctggtagaag atccgttcgc
     4861 gtatatgatg aaacatacaa aatcccacac gaagaaatgc tggaaatgat caaagaagcg
     4921 acgactgcac cttcttctgt caatatgcaa ccatggcgct ttgtggtcgc tgaaagcgag
     4981 gaagcaaaag aaatcttgcg tcctttgatc cgtttcaata cgagacaaaa tgatacttct
     5041 tctgcaatgg tcatgatttt tggcgatatg caatgttacg aatatggcga agaaatctat
     5101 aaccaagctt acgaatcagg gaagatgcca aaagaagtca gagatcaaca attagctgcg
     5161 atcattcctt attacaagag tttttcaaga gaagaaatga acgatgtagt caaagtcgat
     5221 tctagcttag cggctatgca attcatgtta gttgcgcgtg atcatggcta tgaaacaaat
     5281 ccaattggtg gattcgaagc agatcagttg gcagaggcat ttggtttgga taaagaccgt
     5341 tatgttccag ttattatttt atcaatagga aaagcggtag aagaaggtta cgagtctgtt
     5401 cgtttagcac cagaaaagat cactacgttc gaataaaagg ttttcagtta ctattcagta
     5461 gctattgctt tttttgaatc gtgtgtggta acctaataaa aaaggaggtc ggaaaaatga
     5521 ctgcatcgat gaaattacgt ttcgaacttt tgaataacaa ctaaataaat tcaaaagaca
     5581 tgtataaaca acaatttcat ggatggagta tgtccatttt ttcgaacgac agtagaatag
     5641 gtgaaagcta tgacagattt ggtgatttag tcatagttat ttcgttcaga gtaggaaaag
     5701 atgtgatgca gtttttttgc aaacagattt ttctgctctt ttttagtatc gataattgtt
     5761 gttttacatg aaataggagt gtaaaacata tggaaaattt agcagtaaat ataacaaatc
     5821 tacaagtcag ctttggcaac cagctagaac tatctattga ttctcttcgt gtctatcagc
     5881 aagatcgtat agggatcata ggagaaaatg gtgtcggtaa atcgactttg ctcaaactaa
     5941 tagccggtga actttttccc gatcatggga aaatccaaac agagatcacc ttcaactacc
     6001 tgcctcaatt aacctatctc gctgaagcaa aggacctaaa tttggaatta gccagtcatt
     6061 tccagttaag actggaagaa acttcagagc ggaaatggag cggaggggaa gaacgaaaga
     6121 tcgagttgat acgtcttctt tcttcttatg aacaagggat gcttctagac gagccaacaa
     6181 cccatctaga tagaaaaagt attgatcgac tgattgaaga gcttcgttat tattatggta
     6241 cgctggtttt tgttagtcat gatcgctatt ttctagatga gctagcatcg aaaatctggg
     6301 aagtaaaaga cggagaaatc cgagagtttt cggggaatta tagtgcctat ctcactcaaa
     6361 aggaattgga gaaaaagact cagctacgag aagcagagtc gatcatgaaa gagaaaaaac
     6421 gattggaaaa atcgatccaa gaaaagaaaa aacaagcgga aaagttggaa aaagtgtcca
     6481 gtaaaaagaa aaagcaacaa atcagaccgg atcggttgtc ttcctctaaa caaaaagaca
     6541 gtgtacaaaa agccatccaa aagaatgcga aaacattaga gagaagactc caaaaaatag
     6601 gagaaacaac caagccgcag cagatgaaac aaatccgttt tccagtacca aaatctcttg
     6661 aactccacag ccgctatccc atcatgggac aaaatgtcca attggaaaga agtgggagaa
     6721 cattactggt aaatggtgat tttcagtttt ctttaggtaa aaaaatcgcg attgtcggtg
     6781 aaaatggttc aggtaagaca accttattag aacatatccg caaacaagga gaaggaatcc
     6841 ttctctctcc gaaagtaagc tttcaagtat atcagcaaaa gggttatcaa atgacatctg
     6901 aagaatccat cattcgtttt gtcatgagac aaacagagtt ttcagaatcg cttgtccgta
     6961 gtttgctgaa tcacttaggg tttgctcagg aaactttgac gaaaccgtta tgtacattaa
     7021 gtgggggaga agcgacccgt ctgacgattg ctttgctttt tactaagcca agtaatgtgt
     7081 tgctgttaga tgaaccgact aattttattg atatggcaac gatcgaagct ttagagaagc
     7141 taatgcaaat atatccggga acgattttgt ttacttctca tgattcttac tttgtcgagc
     7201 gtacggctga tgaagtttat gaaataaaag ggcagaaaat aaaaaaagta cttacgagaa
     7261 atttttaaat gtgctacgat gattttcgtg acaaatttaa aggagtggta cagattgcct
     7321 acaaataaaa aagaaggaat aatttttaca acaatgatgt gttttttgat ggtcttgggg
     7381 atgagcgcgt ataatttatg gctgcatcaa gatcttcatt tatcaagttt gttgattggt
     7441 ctagtgcctg gattcgtggt ggctttcatt ttggatgtct tcatcatcgg tgtgctggcg
     7501 aaaaaaattg cctttaaact accagtaaat ccagaaagca aggtccaaat gatcttaact
     7561 atttcatgct tgatggtttt aggaatggtg acatgtatgt ctttattcgg tgtgatcatg
     7621 gaacaaggaa tacctgatca attattctct gcttatatga atgcttggaa aatgaatgtg
     7681 attgccgctt taccgttgca gttgatcatc gttggcccgc tttcaagaag tgtgctaaaa
     7741 gcaattcaaa gtaatgaatg atggaacact gttatttgaa aaaaggcctt ggcatgcttt
     7801 ttgtcatagt ctcttgaatg cagttctaga aaaatagtga tccgaacgat aggttttggc
     7861 ctgtcgttcg gatcgctttt tcttttccaa ctgtattgct cttttattga agataatttg
     7921 ctcttcaata gatatttcta gaaaggttct gtttatccct tactcaaaaa actgctgcat
     7981 ttgttctctt acttgctgac accattggag ttcctgctga caaagcataa ttctgcgctg
     8041 gatcacaaca taacgtccaa attcttgttt cttgtctagc gagaggtcat tcaatttttt
     8101 ttgatttttt tgcatgattt tgttcagctg ttgttctcta gtgaatagca gtccagtagc
     8161 tgttgtttta tcgagcgtcg aaaaagcata gatttttgct agaaattcat ctttttgcgt
     8221 aggagcggga gtttcctctt tgatccattc ctctacaaga gcacgtcctt catcagtcaa
     8281 gtggtagatc tttttttgag agtgctcgtc tttgtccaaa acggtcacat aaccctgctg
     8341 gtctaacttg gcaagcgttg tatagatctg actgtgatgg gcttcccaaa aaattgtgat
     8401 cagctgcttc aattcatacc cagaacgtgg tttggataaa agcacgcata ataatatata
     8461 ggctaaagta ttcataaaaa tcctacctct cgtcttattt tctttattat atcacagtaa
     8521 agtttccacg ttttacatgt gcatatttac atgtaaaata aatcatgtat gatgctaata
     8581 gagagaaagg gatggaaata ttggagaatc aaatagatat acaagaacaa acaaatgaag
     8641 aaacaaaggg gaaaagttca gcgctagttg tagcagctac ctgtatgatc gcttttatgg
     8701 gagtgggtct agtagaccca atcttaaaaa cgattgctgc acagttaaac gctacgccag
     8761 ctgaaacgac cctgttattt actagttaca tgttagtcac tggtgtggtg atgctattta
     8821 caggtttctt atctagtcgg atcggtttga aaaaacattg atgacaggat tgtttatcat
     8881 cgttgtattc gctggtcttg gcggattatc tgggaatatc ggaatgctga ttgggttacg
     8941 tgccggctgg ggtgtcggga atgctttatt tgtctctact gctttagcaa cgatcgtcag
     9001 tattttagtt ggtaacacag aaaaagcaat catgatgtat gaagcagcac ttggctgcgg
     9061 aatggctgtt ggccctctag tcggtggggt cttgggcagt ggttcatggc gttatccatt
     9121 ttttggggta gcatttttga tgttttgcgg ttttatagcc ctagcggtac ttttacctga
     9181 aactgagaaa ccaaagagaa aagtaggcct ttgggaaggt gtcaaagcgt taagcaatca
     9241 taaacttcgt tcagttggac taatcgcatt gttatataat ttcggattct ttactttact
     9301 tgcgtatgcg ccatttttat tgattggcta ttctgaatta gaagtcggtt ttgtattttt
     9361 tggctggggt gtgcttctgg ctattgcttc gattttcgtt gcaccggctc ttgagcggaa
     9421 gtttacgact tatcggacaa ttttagcagc atttgtctta ttcattattt gtttgctgtt
     9481 attaggagct ggaacttcta tgccagtcct tgtaccgatc agcattgtgt tagcaggatt
     9541 tttccaagga attatcaata cactgttgac gacgattgct atggaaatac caggtctgca
     9601 acgaaacgtt gcttcgtcca gctatagttt tgtccgcttt ttcggtggag ctttagcacc
     9661 atttattgca ggtgtgattg gtgaacatat tggtgaaagc tatgcgtttt attttgcagg
     9721 tgccattgtt cttatcagtg tatgttgtat catttatcac cgtaattatt ttgctacaaa
     9781 tgaagaagaa taagaaatca gaggttgtga aaaagctgtc ctgcatcaag tcataagaac
     9841 agccaaacaa aaatagcttc tcatattttt cg
//
LOCUS       DAZABD010000207         8060 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.guided.097, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000207 DAZABD010000000
VERSION     DAZABD010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8060
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.guided.097"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            128..1564
                     /locus_tag="ACHALG_003016"
     CDS             128..1564
                     /locus_tag="ACHALG_003016"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFN2588291.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            1753..4200
                     /locus_tag="ACHALG_003017"
     CDS             1753..4200
                     /locus_tag="ACHALG_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFN2588292.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            4430..5497
                     /gene="rlmN"
                     /locus_tag="ACHALG_003018"
     CDS             4430..5497
                     /gene="rlmN"
                     /locus_tag="ACHALG_003018"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFN2588293.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            5487..5693
                     /locus_tag="ACHALG_003019"
     CDS             5487..5693
                     /locus_tag="ACHALG_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2588294.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(5690..6793)
                     /locus_tag="ACHALG_003020"
     CDS             complement(5690..6793)
                     /locus_tag="ACHALG_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFN2588295.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            7346..7894
                     /gene="aac(6')"
                     /locus_tag="ACHALG_003021"
     CDS             7346..7894
                     /gene="aac(6')"
                     /locus_tag="ACHALG_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFN2588296.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         2694 a         1375 c         1751 g         2240 t
ORIGIN      
        1 tttcttacta ctcgaaacta agcgactttt tcacaacctc ctttacatat ttttattttg
       61 ttgtggaagg ggtttcgtgt tattctaaaa gaggaacgat tgaaggttat aggaaaggaa
      121 gagatgaatg gaacacaaag aacttacctc atttccaaaa gattttctgt ggggctctgc
      181 ttctgcagcg tatcaagtag aaggagcttg gcaagaagat gggaaaggag aatcagtctg
      241 ggatcgattt gtacggatac ctggaaagac atttaaagga acgaacggtg atttggcagt
      301 agaccactat catcgttata aagaagatat tgctttaatg aaagaacaag gtctgaaagc
      361 ttaccgcttc tctgtagctt ggacacggat ttttcctaat ggacgaggag aagtcaatca
      421 agctgggctc gctttttatg agagattgat cgacgaattg attgaaaacg atattgaacc
      481 tatgttgact ttatatcatt gggatcttcc acaagcattg caagatgaat ataatggttg
      541 ggaatcacgt cagatcattg aagatttcac tcattatgca gaaacattat ttgaggcatt
      601 ccgcgggaaa gtccattact ggattagttt gaatgaacaa aatatcttca cttcattagg
      661 ttatttgtta gcagcacatc cgccaggtgt gacagacccg aaacgaatgt atgaagttaa
      721 ccatattgct aatttagcaa atgcatctgt catcaataaa tttcacgagt tagaaatgcc
      781 tggaaaaatc ggaccaagct ttgcgtatac gccaaattat ccaattgaca gtaatcctga
      841 aaatgttctg gctgctgaaa atgcagaaga cctgatggcc aattattgga tggatgtcta
      901 tatgtgggga aaatatccga ttgctgccat gaggttctta gaagaaaaag gatgggcacc
      961 aacgattgaa gctggcgatg cagaattgct agaatctgct aaaccagatt ttcttgggat
     1021 caattactat caaacggcga cgaatgcatt caatccatta aacggagttg gtgctggaaa
     1081 aatgaatacg acaggaaaaa aaggttcatc agaagaaact ggagttcctg gaatgtataa
     1141 aaaagttgag aatccatttg tcgaacgaac aaattgggac tgggagattg acccagaagg
     1201 attaaggatc ggactgcgtc gtatcacaag ccgttaccgc atgccggtac tgattacgga
     1261 aaatggctta ggagagtatg acaagttgac agaagataaa caaatccatg acgactatcg
     1321 aatcaattac ttacaaagcc acgtcaaagc catcaaagaa gcaatttcgg atggagcaga
     1381 agtcctaggc tattgcacct ggtcttatac agatttgctt agctggctga acggttatca
     1441 aaagcgatat ggatttgtat atgttgatca agacgaaaca caagaaggat ctttggaacg
     1501 atataagaaa gacagttatt actggtacca aaaactgatt gaagaaaatg gagagaacct
     1561 ttagttcaca tacttaaaac aagaaggtac ggctggatat ggccgtgcct tttcttgata
     1621 aaatcattta gaaattaaga aaaactgtaa aataagctga aaaatgaaag aaagcgtcag
     1681 ttctttcaaa caaaacttta gttcccttac ttttttttgt ataatgaaac ttgatgaaat
     1741 gagggataat aaatggcaca aaagaaacgg cctactaaaa aaaggaaaaa gacgaaaaaa
     1801 acgaaaaagc aaaaacagca gcaggaacaa atgctgacga ttggtgttgg gtttgccttg
     1861 attttatttg ctgtcttcgg ctttttaaaa ttaggttttt taggaatatt gattgcaaac
     1921 ggctttcgga tcattgcagg aaatacttac caaatcctct gtcttttatt agccgtatta
     1981 ggattttgga ttgtcattaa gaatacagag ttttcaattg gtaaaaaccg acgctggttt
     2041 ggcggcatac tcttttattt cggtatctta ttattgttac atgcccattt atttggcaaa
     2101 ctccatactg gggaaccaaa tatcatgggg acgacatggg atttgttagc cagtgatata
     2161 aaacaaagtc aagtagacaa taacgttggt ggtgggatga ttggagcaat cctttatcat
     2221 tttacttact tcttgattgc acagcctgga agttatttag cagcagtgct attactggca
     2281 ggaggagcat tcctgttcag taatctggaa ggctatcagc tgttaaatgg tatccaatca
     2341 attggagaac gagtacagga attattggaa ggtgatcctg ccaagcaagc tcgaaagcaa
     2401 gcggcaaagg aagagagaat gaaacagcgc gcagaagcga aagaagcaag gcgcttagct
     2461 gcaaaagaag ctgcagaaaa agaagctgca gaatatgaga agaaaaaagc ggtgagccag
     2521 caaagggaaa atactgctga tgaatggcag gaaccagaaa atacagagcc ggagcagcta
     2581 agttttgttc caatcgacag ttttcaagaa aatatccatc ctgcgaatct agagaaacca
     2641 gtaccagata caccaaaaca gaccaataca gcagaaggtt tttcagatga gttgccagag
     2701 gatgacggta cctcattaga atttgagatt gaagcagaac aggaaaatca agactatgaa
     2761 ttaccgacag ttgatttgct tgattctata ccaactgtgg atcaaagtga tgagtataaa
     2821 aaaattgaaa aaaatatcgg cgtattagaa caaactttcc aaagtttcgg tgtcgatgcc
     2881 aaggtcgtca aagccagctt aggaccatcc gtcacaaaat tcgaagtcca gccggctgta
     2941 ggggtaaaag taagtaaaat tgtgaactta acagatgata tcgcgcttgc tttagcggcg
     3001 aaagatgtcc ggatggaagc accgattcca ggtaaatcac tgattggtat tgaagtacca
     3061 aatggaaaga tcagtatggt ttcctttaga gaaatcatcg aagcacaacc aaaccaccca
     3121 gataaattac tagaagtacc tttaggacgt gatgtttctg gacgggtaca aacggctgat
     3181 ctttcgaaga tgcctcactt actcgtggct ggttcgactg gtagtgggaa gtcagtagca
     3241 attaatggga ttatcacaag tatattaatg cgtgctaagc cccatgaagt aaaactgatg
     3301 atgatcgatc cgaaaatggt tgaattgaat atgtacaacg gcatacctca tctgttgacg
     3361 ccggtcgtga cgaaccctag aaaagcagcg caagccttac aaaaagtagt acaagaaatg
     3421 gaagaacgat atgaaaaatt cgcggcaacc ggagtaagaa atatttctgg ttacaatgaa
     3481 tttgttcaac agaaaaactt ggaaaacgga acgaaacatc caaccttacc attcatcgta
     3541 gtgatcgtag atgaattagc tgatttgatg atggtagcca gcaatgaagt tgaagatgcg
     3601 attattcgtt tagcgcaaat ggctcgggca gcggggattc atatgatcct agctactcaa
     3661 cgtccaagtg tggatgttat cacaggaatc atcaaagcaa atgtaccttc cagaatggca
     3721 tttgcggtat caagcggtac agattctcgg acgatcattg attctaatgg ggcagaaaag
     3781 ctcttagggc gaggagatat gctattcttg ccaatgggtg agaataaacc gatccgtgtg
     3841 caaggagcat tcatttctga ccacgaagta gaacgggtcg ttcaatttgt tacagatcag
     3901 caagaagctc attatgaaga gaaaatgatg ccgacagatg aagttgaaac gacaggtgct
     3961 ccagaacagc cacaagatga gctgtttgaa gaagcaaaag cacttgtcgt tgaaatgcaa
     4021 acggcaagta tttcccttct ccaaagaaga tttcggatcg gttacaaccg agcagcacgt
     4081 ttagtagatg aactggaagc tcatggagtt gttggcccgt cagaaggaag caagcctcgg
     4141 aaagtattca ttgagcagga agaaacatca gaaacgttgc cggacgataa tgacatgtaa
     4201 aacttgtgtg atgaagggtt gaaaaacgaa taaccaataa aaagacaagg catgatgata
     4261 ccaaaagaaa aaatggtttt tttccatctt ttctaatacg gtatgatgtc ttgtcttttt
     4321 agtttttgtg agattaaagt tctattcgat ttatttgtga agtattgcat taaggaaatg
     4381 aatcatgtat agtatgtagc gaaactttga gaaaaagagg gaaaatactt tggaaaaaca
     4441 gtccatttat ggcttgacga atgaagaatt aatcaattgg tttatagaaa acggcgagaa
     4501 aaaattccga gctgcccaag tttgggaatg gttgtatcaa aaacgtgtaa gtaatttcac
     4561 agaaatgacg aatctatcga aacaattgat cgaaaaatta tcggagcatt ttatcatcaa
     4621 tcctctaaaa cagatggtcg tccaagaagc ttcggatggt acagtaaaat atttgtttga
     4681 actccctgat aaaaacatga ttgagactgt actgatgaga caagaatatg gcttgtcagt
     4741 ttgtgtgacg acacaagtag gatgtaatat cggctgtacg ttttgtgcaa gcgggctgct
     4801 caaaaagaac cgtgatctga ctgctggaga aattgttgca cagatcatga tggtccaaca
     4861 ttattttgat gagcgcaatc ttggcgaacg cgtttcacat gtcgttgtaa tggggatcgg
     4921 tgagccattt gataattacg acaatgtaat ggactttttg catatcatca atgatgccaa
     4981 aggattggca attggtgctc ggcacattac agtttccact agtggattag cacacaaaat
     5041 caaggaattc gctaataacg gcttgcaagt caatctggct atttccttgc acgcgccgaa
     5101 taatgaggtg cgaacatcga tcatgcggat caaccgaagt ttcccaattg agaaattgat
     5161 ggaagctgtg gatgagtatt tagagaaaac aaaccgtcga atcacgtttg aatatatcat
     5221 gttgaatcaa gtaaatgata gaccagagca tgcgcaacaa ttagcagatc tattaaaaga
     5281 taagaaaaaa ttagcatatg tcaacttgat tccatataat ccagtcagtg aacatgatca
     5341 atattctcgc agtccaaaag ctgatgtatt gagattttat gatgtattga agaaaaacgg
     5401 cgtaaactgt gtgatacgta aagaacatgg tacagatatt gatgcagctt gcggacaatt
     5461 gcgaagcaag caaatgaaaa aaacagttgc caaataatcg tatcctactc gttttttcaa
     5521 ttaccataaa aggagcagtt ttcttaatta aggaactgtt caacttaaat aagagaaaag
     5581 attctatagc gaaagctata aagctttttt tgacaagaaa aaccctcatc aagcaaagaa
     5641 atgtcctcaa tttgatgaca gcctgcttga tgggggtttt agcaattatt taactgattt
     5701 tcttatatcc ttgctggctt tcttgtgtag catgataagt ttctacgtca agaatggcct
     5761 gtttaaattt ttctcgggtg atttcataag gcacatgttt aatatcattt gttgccatgg
     5821 ctttatctaa gattgcttct aaatcggtcg tgacattgag attcatgtcg gttaacgata
     5881 ctggaagttg gttctttttc ataaacacat agattttatc aaacaaatcg aactgctgat
     5941 ccatcatcaa taaggtcagt acaccataac tgacagatgc tccgtgaagg tatccctcca
     6001 ttttttctag aacagtcgta ccgtaataga atgagtgtgc catagcagaa ttatagttat
     6061 tgttaactaa aacagaaacc aatccggtag taacaatgat atccgaaacg acttgttcaa
     6121 ttgcatatga gggacgatcg tcggcactat ctttcaatgc ttgcgtacca taagtcaaga
     6181 gacgatcaat gcacattttt ccgatttgga ttcctaatct atccgtgtag cctagatcat
     6241 cgccacgagc agaaaaagta gattcgactt ctttggataa agcatcgcca atacctgccc
     6301 ataagtattt atcaggtgct tcggcaataa ttgctgtatc aatgaaggtg tgtacaggtg
     6361 gttttgaagg aaagaagagt ccgtccatta cgccttcttc gttatagatg acacataccg
     6421 cagtgacggg cgaacagttc gaaccaatag tgggaaaggt gaagactggt ttagctagtt
     6481 tttcaccaat gacttttaca gtatcgcaag cacggccacc accgactgca aacaggacgt
     6541 ctgctttttg gacactttca tgctggatca actgttcaat atttccataa gttgaattac
     6601 ccccgtacca gagactggcg acaacctcta tttcctgtgt caaagctact tttattttgt
     6661 tttctgcctt tgaaagtgca gtcttccctc caatcagcac tacttttgtc ccatattctt
     6721 tgacgattcc atttgctttt tcatacgcat ctgttccaat ggtataattt ggtaagaaaa
     6781 cactttgact catattcatt tccttctttc ttcgttgaaa cgggttaaaa ttttaaagag
     6841 tgatcgatct gactgcggct gtagatggag tagaggtgaa cctaagacag gataagtgat
     6901 gaataaggtg gcatgaacac cttgaaatca tcaataagta tcctaaaaac gaacaagtac
     6961 aaaaacattg aaaacgacaa taatgaattt ttgttcctct tagtatttct accgattccc
     7021 tttgcttttt tgtaattatc taaaaattct aaaaattaag attattatat gaaaacaggt
     7081 tttatttgtc aattcataat tggttttttt taaagcaaaa gataatctat atcatgatga
     7141 acaaacagag ttttttcctc caattgatgg ctttcctgat ctttcctatt ttgaataaag
     7201 tgtttctgta taattttttt attttataag taagataaat aatgaaagaa tagagatact
     7261 gattggtagc taaagatttt tgagcgagaa catttcgttt tttcaaaagg actgttagaa
     7321 taagcaagaa caagggggaa gacgtatgat aatcagtgaa tttgaccgta ataatccagt
     7381 attgaaagat cagctttctg atttactgag actgacttgg ccggaagaat atggagacag
     7441 ctcggcagaa gaagtagaag aaatgatgaa tccagaacga atcgcggtag cagcggtaga
     7501 ccaagatgag ttagtaggat ttattggtgc aatccctcaa tacggtatca caggttggga
     7561 attgcatcca ttagttgtag aaagctcccg acgaaagaac caaataggta ctcgattagt
     7621 caattactta gaaaaagaag tagcttccag aggaggaatc acgatttatt taggtacgga
     7681 tgatttagac catggaacaa cgttaagtca aacggacctg tatgagcata catttgataa
     7741 agtggcttcc atccagaacc ttcgtgaaca tccgtatgaa ttctatgaaa aattaggtta
     7801 taaaatcgta ggtgtcttac caaatgcaaa tggctgggac aaaccggata tttggatggc
     7861 aaaaacgatt attcctcgac caaattctca ataataaaaa gaggttgtga cagaagtaat
     7921 cagcttcaag aaataagaag gaatttacga aaattacttc ttgaattttt gtgaaattcc
     7981 aacttatttc cgaagaagct acttttggaa caccgtgtac cagaggttgt gaaaaagtcg
     8041 cttagtttcg agtagtaaga
//
LOCUS       DAZABD010000208         7250 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.guided.104, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000208 DAZABD010000000
VERSION     DAZABD010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7250
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.guided.104"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..572
                     /locus_tag="ACHALG_003022"
     CDS             <1..572
                     /locus_tag="ACHALG_003022"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN2588297.1"
                     /translation="DFILYLKLCNRTYRSYSKEFKQKIVAEYLQTGYGFSYLAAKYNI
                     PSKTTVNKWVIRYTEGKENETYSPKSEVYNMTGVKKSYEEKLKIVEDYIANRLTYLEA
                     AEKNHVSYSNIYSWVNKYKKHGPIGLEDNRGRGKPTEIQTTEERLNAEVETLKARNKW
                     LEMENDALKKRRKIAGNHKSQGLDKKWNI"
     gene            560..1420
                     /locus_tag="ACHALG_003023"
     CDS             560..1420
                     /locus_tag="ACHALG_003023"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFN2588298.1"
                     /translation="MEYLTIEELKHKYPVIHLCDILGIAKSSYYKWLKREPSETELKR
                     LKLMRAIKGIHEAFGGIYGYRRMTIFLNFFRRAKVNHKCVHRLMRIMGITAVIRRKRR
                     NYVPHKAVHVAENILNRDFHAERPMEKLLTDVTEFRLTNGTKRYLSAIYDLGSKKIVA
                     YKTSHRNDNPLVLDTLKQILGDVKPETTLIHSDRGSQYTSHAFNKMIKDHQIIHSMSR
                     VSKCIDNGPMEGFWGTLKVEMFNLDTFDRPAYLDRKIKAYIAFFNNERVTLDMGLAIP
                     TEEKILQMIA"
     gene            complement(1505..4429)
                     /locus_tag="ACHALG_003024"
     CDS             complement(1505..4429)
                     /locus_tag="ACHALG_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HFN2588299.1"
                     /translation="MKIARGRELLTPEQRQAFMQIPEDEWILGTYFTFSKRDLEIVNK
                     RRREENRLGFAVQLAVLRYPGWPYTHIKSIPDSVIQYISKQIGVSPSSLDHYPQRENT
                     LWDHLKEIRSEYDFVTFTLSEYRMTFKYLHQLALENGDAIHLLHECIDFLRKNKIILP
                     AITTLERMVWEARAMAEKKLFNTVSKSLTNEQKEKLEGIITSQHPSESNKTILGWLKE
                     PPGHPSPETFLKIIERLEYIRGMDLETVQISHLHRNRLLQLSRLGSRYEPYAFRDFQE
                     NKRYSILTIYLLQLTQELTDKAFEIHDRQILSLLSKGRKAQEEIQKQNGKKLNEKVIH
                     FTNIGQALIKAREEKLDVFKVLESVIEWNTFVSSVEEAQELARPADYDYLDLLQKRFY
                     SLRKYTPTLLRVLEFHSTKANEPLLQAVEIIRGMNESGKRKVPDDSPVDFISKRWKRH
                     LYEDDGTTINRHYYEMAVLTELREHVRAGDVSIVGSRQYRDFEEYLFSEDTWNQSKGN
                     TRLSVSLSFEDYITERTSSFNERLKWLAANSNKLDGVSLEKGKLSLARLEKDVPEEAK
                     KFSASLYQMLPRIKLTDLLMDVAHITGFHEQFTHASNNRKPDKEETIIIMAALLGMGM
                     NIGLSKMAEATPGLTYKQLANVSQWRMYEDAMNKAQAILVNFHHKLQLPFYWGDGTTS
                     SSDGMRMQLGVSSLHADANPHYGTGKGATIYRFTSDQFSSYYTKIIHTNSRDAIHVLD
                     GLLHHETDLNIEEHYTDTAGYTDQIFGLTHLLGFKFAPRIRDLSDSKLFTIDKASEYP
                     KLEAILRGQINTKVIKENYEDVLRLAHSIREGTVSASLIMGKLGSYSRQNSLATALRE
                     MGRIEKTIFILNYISDESLRRKIQRGLNKGEAMNGLARAIFFGKQGELRERTIQHQLQ
                     RASALNIIINAISIWNTLHLTTAVEYKKRTGSFNEDLLHHMSPLGWEHINLLGEYHFN
                     SE"
     gene            4575..5150
                     /locus_tag="ACHALG_003025"
     CDS             4575..5150
                     /locus_tag="ACHALG_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFN2588300.1"
                     /translation="MRKIGYIRVSSTNQNPSRQFQQLNEIGMDIIYEEKVSGATKDRE
                     QLQKVLDDLQEDDIIYVTDLTRITRSTQDLFELIDNIRDKKASLKSLKDTWLDLSEDN
                     PYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKL
                     YKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            5364..6059
                     /gene="vanR-A"
                     /locus_tag="ACHALG_003026"
     CDS             5364..6059
                     /gene="vanR-A"
                     /locus_tag="ACHALG_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFN2588301.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            6098..7192
                     /gene="vanS"
                     /locus_tag="ACHALG_003027"
     CDS             6098..7192
                     /gene="vanS"
                     /locus_tag="ACHALG_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000791309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS"
                     /protein_id="HFN2588302.1"
                     /translation="MYIVAIVVVAIVFVLYIRSMIRGKLGDWILSILENKYDLNHLDA
                     MKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKYFDEINTGIDVLIQNEDKQIE
                     LSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVMYLAHDIKTPLTSIIGYLSLL
                     DEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRYNLQTITLTKTHIDLYYMLVQ
                     MTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFNNILKNAAAYSEDNSIIDITA
                     GLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSSDTGGAGLGLAIAKEIIVQHG
                     GQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT         2303 a         1387 c         1412 g         2148 t
ORIGIN      
        1 gggattttat tctttattta aaactttgca acagaaccta ccgcagttac tcgaaagaat
       61 tcaagcagaa aattgtagct gaatacctac agacgggtta cggtttttca tacttagccg
      121 ctaaatataa cattccatct aaaacaacgg taaacaaatg ggtaattcga tatactgaag
      181 gaaaagagaa tgagacatat tctccgaaat ctgaggtgta caacatgacg ggtgttaaaa
      241 aatcatatga agaaaaatta aaaatcgtag aagattacat agccaatcgc ctgacttatc
      301 tggaagcagc ggagaaaaac catgtaagtt acagtaacat ttattcctgg gtgaataaat
      361 acaaaaagca tggaccgata ggtctggaag acaaccgggg ccgcgggaag cccacggaaa
      421 ttcaaacaac ggaagaacgt ttaaacgcag aagtagagac actcaaagcc cggaacaaat
      481 ggctagaaat ggagaacgat gcgttaaaaa agcgccgaaa aatcgctggg aatcacaaat
      541 cacaggggtt agacaagaag tggaatattt aactattgaa gaattaaagc ataaatatcc
      601 cgttatccat ctttgtgaca tactgggtat cgccaaatcc agctactata agtggttaaa
      661 acgggaaccc tcagaaacag aattaaaacg cctgaaactg atgcgggcga tcaaaggaat
      721 ccacgaggca ttcggtggga tttacggcta ccgaagaatg accatctttc tcaacttttt
      781 tagaagagcg aaagtgaatc ataagtgtgt acaccgcctc atgagaatca tggggatcac
      841 agccgtcatc cgtcgcaaaa gaaggaacta cgtgccacac aaagccgtac atgtggctga
      901 aaacatctta aaccgtgatt tccacgctga aagacccatg gaaaagttat tgacggatgt
      961 cacggaattc cggttgacca atgggacaaa acgttacctg agcgctattt atgacctcgg
     1021 gtcaaagaaa atcgtggctt ataaaaccag tcaccgcaat gacaacccgt tagtactgga
     1081 tacactaaag cagattttgg gtgatgtaaa gcctgaaacc acactgattc atagcgaccg
     1141 cggttcccag tacacctccc atgcctttaa caagatgatt aaagatcacc agataatcca
     1201 tagtatgtca cgcgtctcga aatgtattga caacggccct atggaaggct tttggggtac
     1261 cctcaaggtg gagatgttta acctggatac gtttgacaga ccagcgtact tagaccggaa
     1321 aatcaaggca tacatcgcgt tcttcaacaa tgaacgcgtt actttggata tgggattagc
     1381 aattcctacg gaagagaaga ttctacaaat gattgcataa acgctacaaa aaaagaccac
     1441 acatttttac atgcatggtc taaaatactt tgttttttta cctgtctact tgacaggggg
     1501 cagttcactc tgagttaaaa tggtattctc ctagtaaatt aatatgttcc caacctaagg
     1561 gcgacatatg gtgtaacaaa tcttcattaa agctacctgt ccgtttttta tattcaactg
     1621 ctgttgttag gtggagagta ttccaaatac ttatagcatt gataattatg tttaaagcac
     1681 tggctctttg caattgatgc tgtatggtgc gttctctaag ctcaccttgt tttccgaaga
     1741 aaatagctct tgccaatcca ttcatggctt ctcctttatt caatcctctt tgtatttttc
     1801 ttcttaatga ttcatccgat atataattca aaataaagat cgttttttct attcggccca
     1861 tctcacgtaa ggctgtagct aagctgtttt gtcttgaata ggaacctagc ttccccataa
     1921 taagggatgc tgaaactgtt ccctccctta tagaatgagc taatcgcaaa acatcctcat
     1981 aattttcttt aatgaccttt gtatttattt gtccacgtaa aatggcttct agttttggat
     2041 actcacttgc tttatctatc gtaaataatt ttgagtccga taaatccctt attcttgggg
     2101 caaatttaaa tcctaataaa tgagtcagtc cgaatatttg gtcagtgtaa ccggcagtgt
     2161 ctgtataatg ttcctctatg tttagatccg tctcatgatg taacaaacca tccaaaacat
     2221 gaatcgcatc tcttgaatta gtatgaataa tctttgtgta gtaagaagag aattgatcac
     2281 ttgtaaatcg gtagatggtg gctccttttc cagttccata atgtggattt gcatctgcat
     2341 gtagtgatga aacacctagc tgcattctca taccatctga cgaagatgtt gtaccgtcgc
     2401 cccaatagaa aggcaattgt aatttatgat gaaagtttac taatatggct tgggctttat
     2461 tcatggcatc ttcatacatg cgccattgag atacattggc tagttgctta tatgtaagtc
     2521 cgggtgtggc ttcggccatc ttgctcaagc caatattcat tcccattcct aaaagggcag
     2581 ccatgataat gattgtttct tccttatctg gttttcgatt attggaagca tgagtgaatt
     2641 gctcatgaaa tcctgttata tgggccacat ccatgagtaa atcagttaat tttattcttg
     2701 gtagcatctg ataaaggctt gcactaaatt tttttgcttc ttctggaaca tctttttcta
     2761 agcgtgcaag tgatagcttt cctttttcaa gagaaacccc atctaactta ttggaattgg
     2821 cagctaacca ctttaacctt tcattaaagc tgctggttct ctccgttata taatcttcga
     2881 atgataaact aactgataat ctcgtattcc ccttcgattg attccatgta tcttccgaaa
     2941 acaaatattc ctcaaaatcc ctatattgtc tgctgccaac aatggaaaca tctcctgccc
     3001 gaacatgctc ccgaagttct gttaaaacag ccatttcata gtaatgacga ttaattgttg
     3061 taccatcatc ctcgtataaa tgtcttttcc atcgttttga aataaaatcc acaggtgagt
     3121 catcaggcac ttttcgcttt ccagattcgt tcattcctcg gataatctca acagcttgta
     3181 aaagtggctc atttgccttt gtagaatgaa attccaatac tcttaatagc gttggcgtat
     3241 attttcttag tgaataaaac cgtttttgca gtaagtctaa ataatcatag tcggcaggac
     3301 gtgcaagttc ctgagcctct tctactgaag agacaaaggt attccattca ataaccgatt
     3361 ctaaaacctt aaaaacgtct aatttttcct ctcttgcttt aattaatgct tgtccgatgt
     3421 tcgtaaagtg tataactttc tcatttagct ttttaccgtt ttgtttctgg atttcctctt
     3481 gagccttacg accttttgat aacaaactaa gtatttgcct atcatgaatt tcaaacgctt
     3541 tatccgttag ctcctgagta agttgtaata aatagatggt taatatcgaa taacgtttat
     3601 tttcttgaaa gtcacggaat gcatacggct cgtatcttga gcctaagcga gacagctgca
     3661 acaggcggtt acggtgcaaa tgactaattt gcactgtttc taaatccatt cctcgtatgt
     3721 attcgagtcg ttctattatt tttagaaaag tttcgggtga aggatgaccc ggtggctctt
     3781 ttaaccaacc caatatcgtt ttattggatt cggatggatg ctgcgaggta ataatccctt
     3841 caagcttttc tttttgctca tttgttagag atttactaac cgtattaaat agcttctttt
     3901 cagccattgc ccttgcttcc cacaccattc tttcaagtgt agtgatagca ggcagtataa
     3961 ttttgttttt tcttagaaaa tctatgcatt catgcagtag atgaatggca tcaccatttt
     4021 ccaaagctaa ttgatgaagg tacttaaatg tcattcgata ttcactcagg gtaaaagtta
     4081 caaagtcgta ttcacttcga atttctttca aatgatccca aagtgtattt tccctttgag
     4141 gataatgatc aagcgaggat ggactaacac caatctgttt cgatatatat tgtatgaccg
     4201 aatctgggat gcttttgata tgagtgtatg gccaaccggg ataccgaaga acagctaatt
     4261 gaacagcaaa tcctaaacgg ttttcttccc tccttcgctt attaactatt tctaaatccc
     4321 gtttggaaaa agtgaagtag gtccccagta tccattcatc ttcagggatt tgcataaaag
     4381 cctgtctctg ttccggtgta agcaattctc tacctctcgc aattttcatt cagtatcatt
     4441 ccatttctgt attttcaatt tattagttca attatatatc aatagagtgt actctattga
     4501 tacaaatgta gtagactgat aaaatcatag ttaagagcgt ctcataagac ttgtctcaaa
     4561 aatgaggtga tattttgcgg aaaatcggtt atattcgtgt cagttcgact aaccagaatc
     4621 cttcaagaca atttcagcag ttgaacgaga tcggaatgga tattatatat gaagagaaag
     4681 tttcaggagc aacaaaggat cgcgagcaac ttcaaaaagt gttagacgat ttacaggaag
     4741 atgacatcat ttatgttaca gacttaactc gaatcactcg tagtacacaa gatctatttg
     4801 aattaatcga taacatacga gataaaaagg caagtttaaa atcactaaaa gatacatggc
     4861 ttgatttatc agaagataat ccatacagcc aattcttaat tactgtaatg gctggtgtta
     4921 accaattaga gcgagatctt attcggatga gacaacgtga agggattgaa ttggctaaga
     4981 aagaaggaaa gtttaaaggt cgattaaaga agtatcataa aaatcacgca ggaatgaatt
     5041 atgcggtaaa gctatataaa gaaggaaata tgactgtaaa tcaaatttgt gaaattacta
     5101 atgtatctag ggcttcatta tacaggaaat tatcagaagt gaataattag ccattctgta
     5161 ttccgctaat gggcaatatt tttaaagaag aaaaggaaac tataaaatat taacagcctc
     5221 ctagcgatgc cgaaaagccc tttgataaaa aaagaatcat catcttaaga aattcttagt
     5281 catttattat gtaaatgctt ataaattcgg ccctataatc tgataaatta ttaagggcaa
     5341 acttatgtga aagggtgata actatgagcg ataaaatact tattgtggat gatgaacatg
     5401 aaattgccga tttggttgaa ttatacttaa aaaacgagaa ttatacggtt ttcaaatact
     5461 ataccgccaa agaagcattg gaatgtatag acaagtctga gattgacctt gccatattgg
     5521 acatcatgct tcccggcaca agcggcctta ctatctgtca aaaaataagg gacaagcaca
     5581 cctatccgat tatcatgctg accgggaaag atacagaggt agataaaatt acagggttaa
     5641 caatcggcgc ggatgattat ataacgaagc cctttcgccc actggagtta attgctcggg
     5701 taaaggccca gttgcgccga tacaaaaaat tcagtggagt aaaggagcag aacgaaaatg
     5761 ttatcgtcca ctccggcctt gtcattaatg ttaacaccca tgagtgttat ctgaacgaga
     5821 agcagttatc ccttactccc accgagtttt caatactgcg aatcctctgt gaaaacaagg
     5881 ggaatgtggt tagctccgag ctgctatttc atgagatatg gggcgacgaa tatttcagca
     5941 agagcaacaa caccatcacc gtgcatatcc ggcatttgcg cgaaaaaatg aacgacacca
     6001 ttgataatcc gaaatatata aaaacggtat ggggggttgg ttataaaatt gaaaaataaa
     6061 aaaaaacgac tattccaaac tagaacgaaa actttacatg tatatcgttg caattgttgt
     6121 ggtagcaatt gtattcgtgt tgtatattcg ttcaatgatc cgagggaaac ttggggattg
     6181 gatcttaagt attttggaaa acaaatatga cttaaatcac ctggacgcga tgaaattata
     6241 tcaatattcc atacggaaca atatagatat ctttatttat gtggcgattg tcattagtat
     6301 tcttattcta tgtcgcgtca tgctttcaaa attcgcaaaa tactttgacg agataaatac
     6361 cggcattgat gtacttattc agaacgaaga taaacaaatt gagctttctg cggaaatgga
     6421 tgttatggaa caaaagctca acacattaaa acggactctg gaaaagcgag agcaggatgc
     6481 aaagctggcc gaacaaagaa aaaatgacgt tgttatgtac ttggcgcacg atattaaaac
     6541 gccccttaca tccattatcg gttatttgag cctgcttgac gaggctccag acatgccggt
     6601 agatcaaaag gcaaagtatg tgcatatcac gttggacaaa gcgtatcgac tcgaacagct
     6661 aatcgacgag ttttttgaga ttacacggta taacctacaa acgataacgc taacaaaaac
     6721 gcacatagac ctatactata tgctggtgca gatgaccgat gaattttatc ctcagctttc
     6781 cgcacatgga aaacaggcgg ttattcacgc ccccgaggat ctgaccgtgt ccggcgaccc
     6841 tgataaactc gcgagagtct ttaacaacat tttgaaaaac gccgctgcat acagtgagga
     6901 taacagcatc attgacatta ccgcgggcct ctccggggat gtggtgtcaa tcgaattcaa
     6961 gaacactgga agcatcccaa aagataagct agctgccata tttgaaaagt tctataggct
     7021 ggacaatgct cgttcttccg atacgggtgg cgcgggactt ggattggcga ttgcaaaaga
     7081 aattattgtt cagcatggag ggcagattta cgcggaaagc aatgataact atacgacgtt
     7141 tagggtagag cttccagcga tgccagactt ggttgataaa aggaggtcct aagagatgta
     7201 tataattttt taggttctgt tgcaaagttt taaataaaga ataaaatccc
//
LOCUS       DAZABD010000209         6475 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.guided.111, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000209 DAZABD010000000
VERSION     DAZABD010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.guided.111"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..932
                     /gene="vanA"
                     /locus_tag="ACHALG_003028"
     CDS             <1..932
                     /gene="vanA"
                     /locus_tag="ACHALG_003028"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase"
                     /protein_id="HFN2588303.1"
                     /translation="KYEPLYIGITKSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGL
                     LVKKNHEYEINHVDVAFSALHGKSGEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLT
                     YIVAKNAGIATPAFWVINKDDRPVAATFTYPVFVKPARSGSSFGVKKVNSADELDYAI
                     ESARQYDSKILIEQAVSGCEVGCAVLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKG
                     SENAVITVPADLSAEERGRIQETAKKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLP
                     GFTSYSRYPRMMAAAGIALPELIDRLIVLALKG"
     gene            938..1546
                     /locus_tag="ACHALG_003029"
     CDS             938..1546
                     /locus_tag="ACHALG_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HFN2588304.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
     gene            1974..2885
                     /gene="vanY"
                     /locus_tag="ACHALG_003030"
     CDS             1974..2885
                     /gene="vanY"
                     /locus_tag="ACHALG_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HFN2588305.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYELWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            3038..3523
                     /gene="vanZ-A"
                     /locus_tag="ACHALG_003031"
     CDS             3038..3523
                     /gene="vanZ-A"
                     /locus_tag="ACHALG_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HFN2588306.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(3810..4139)
                     /locus_tag="ACHALG_003032"
     CDS             complement(3810..4139)
                     /locus_tag="ACHALG_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HFN2588307.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
     gene            4444..4773
                     /locus_tag="ACHALG_003033"
     CDS             4444..4773
                     /locus_tag="ACHALG_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFN2588308.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            4763..5050
                     /locus_tag="ACHALG_003034"
     CDS             4763..5050
                     /locus_tag="ACHALG_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFN2588309.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KTGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPILLCGTHENFYEQLKKIR"
     gene            complement(5329..5892)
                     /locus_tag="ACHALG_003035"
     CDS             complement(5329..5892)
                     /locus_tag="ACHALG_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFN2588310.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAV
                     RPQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTP
                     HGRLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVDRAVKLYKSQ
                     VYSVKEITEMTGISKATLYRYLKDNRE"
BASE COUNT         2174 a         1049 c         1239 g         2013 t
ORIGIN      
        1 aaaaatacga gccgttatac attggaatta cgaaatctgg tgtatggaaa atgtgcgaaa
       61 aaccttgcgc ggaatgggaa aacgacaatt gctattcagc tgtactctcg ccggataaaa
      121 aaatgcacgg attacttgtt aaaaagaacc atgaatatga aatcaaccat gttgatgtag
      181 cattttcagc tttgcatggc aagtcaggtg aagatggatc catacaaggt ctgtttgaat
      241 tgtccggtat cccttttgta ggctgcgata ttcaaagctc agcaatttgt atggacaaat
      301 cgttgacata catcgttgcg aaaaatgctg ggatagctac tcccgccttt tgggttatta
      361 ataaagatga taggccggtg gcagctacgt ttacctatcc tgtttttgtt aagccggcgc
      421 gttcaggctc atccttcggt gtgaaaaaag tcaatagcgc ggacgaattg gactacgcaa
      481 ttgaatcggc aagacaatat gacagcaaaa tcttaattga gcaggctgtt tcgggctgtg
      541 aggtcggttg tgcggtattg ggaaacagtg ccgcgttagc tgttggcgag gtggaccaaa
      601 tcaggctgca gtacggaatc tttcgtattc atcaggaagt cgagccggaa aaaggctctg
      661 aaaacgcagt tataaccgtt cccgcagacc tttcagcaga ggagcgagga cggatacagg
      721 aaacggcaaa aaaaatatat aaagcgctcg gctgtagagg tctagcccgt gtggatatgt
      781 ttttacaaga taacggccgc attgtactga acgaagtcaa tactctgccc ggtttcacgt
      841 catacagtcg ttatccccgt atgatggccg ctgcaggtat tgcacttccc gaactgattg
      901 accgcttgat cgtattagcg ttaaaggggt gataagcatg gaaataggat ttactttttt
      961 agatgaaata gtacacggtg ttcgttggga cgctaaatat gccacttggg ataatttcac
     1021 cggaaaaccg gttgacggtt atgaagtaaa tcgcattgta gggacatacg agttggctga
     1081 atcgcttttg aaggcaaaag aactggctgc tacccaaggg tacggattgc ttctatggga
     1141 cggttaccgt cctaatcgtg ctgtaaactg ttttatgcaa tgggctgcac agccggaaaa
     1201 taacctgaca aaggaaagtt attatcccaa tattgaccga actgagatga tttcaaaagg
     1261 atacgtggct tcaaaatcaa gccatagccg cggcagtgcc attgatctta cgctttatcg
     1321 attagacacg ggtgagcttg taccaatggg gagccgattt gattttatgg atgaacgctc
     1381 tcatcatgcg gcaaatggaa tatcatgcaa tgaagcgcaa aatcgcagac gtttgcgctc
     1441 catcatggaa aacagtgggt ttgaagcata tagcctcgaa tggtggcact atgtattaag
     1501 agacgaacca taccccaata gctattttga tttccccgtt aaataaactt ttaaccgttg
     1561 cacggacaaa ctatataagc taactctttc ggcaggaaac ccgacgtatg taactggttc
     1621 ttagggaatt tatatatagt agatagtatt gaagatgtaa ggcagagcga tattgcggtc
     1681 attatctgcg tgcgctgcgg caagatagcc tgataataag actgatcgca tagaggggtg
     1741 gtatttcaca ccgcccattg tcaacaggca gttcagcctc gttaaattca gcatgggtat
     1801 cacttatgaa aattcatcta cattggtgat aatagtaaat ccagtagggc gaaataattg
     1861 actgtaattt acggggcaaa acggcacaat ctcaaacgag attgtgccgt ttaaggggaa
     1921 gattctagaa atatttcata cttccaacta tatagttaag gaggagactg aaaatgaaga
     1981 agttgttttt tttattgtta ttgttattct taatatactt aggttatgac tacgttaatg
     2041 aagcactgtt ttctcaggaa aaagtcgaat ttcaaaatta tgatcaaaat cccaaagaac
     2101 atttagaaaa tagtgggact tctgaaaata cccaagagaa aacaattaca gaagaacagg
     2161 tttatcaagg aaatctgcta ttaatcaata gtaaatatcc tgttcgccaa gaaagtgtga
     2221 agtcagatat cgtgaattta tctaaacatg acgaattaat aaatggatac gggttgcttg
     2281 atagtaatat ttatatgtca aaagaaatag cacaaaaatt ttcagagatg gtcaatgatg
     2341 ctgtaaaggg tggcgttagt cattttatta ttaatagtgg ctatcgagac tttgatgagc
     2401 aaagtgtgct ttaccaagaa atgggggctg agtatgcctt accagcaggt tatagtgagc
     2461 ataattcagg tttatcacta gatgtaggat caagcttgac gaaaatggaa cgagcccctg
     2521 aaggaaagtg gatagaagaa aatgcttgga aatacgggtt cattttacgt tatccagagg
     2581 acaaaacaga gttaacagga attcaatatg aactatggca tattcgctat gttggtttac
     2641 cacatagtgc gattatgaaa gaaaagaatt tcgttctcga ggaatatatg gattacctaa
     2701 aagaagaaaa aaccatttct gttagtgtaa atggggaaaa atatgagatc ttttattatc
     2761 ctgttactaa aaataccacc attcatgtgc cgactaatct tcgttatgag atatcaggaa
     2821 acaatataga cggtgtaatt gtgacagtgt ttcccggatc aacacatact aattcaagga
     2881 ggtaaggatg gcggaatgaa accaacgaaa ttaatgaaca gcattattgt actagcactt
     2941 ttggggtaac gttagctttt taatttaaaa cccacgttaa ctaggacatt gctatactaa
     3001 tgatacaact taaacaaaag aattagagga aattatattg ggaaaaatat tatctagagg
     3061 attgctagct ttatatttag tgacactaat ctggttagtg ttattcaaat tacaatacaa
     3121 tattttatca gtatttaatt atcatcaaag aagtcttaac ttgactccat ttactgctac
     3181 tgggaatttc agagagatga tagataatgt tataatcttt attccatttg gcttgctttt
     3241 gaatgtcaat tttaaagaaa tcggattttt acctaagttt gcttttgtac tggttttaag
     3301 tcttactttt gaaataattc aatttatctt cgctattgga gcgacagaca taacagatgt
     3361 aattacaaat actgttggag gctttcttgg actgaaatta tatggtttaa gcaataagca
     3421 tatgaatcaa aaaaaattag acagagttat tatttttgta ggtatacttt tgctcgtatt
     3481 attgctcgtt taccgtaccc atttaagaat aaattacgtg taagatgtct aaatcaagca
     3541 atctgatctt tcatacacat aaagatattg aatgaattgg attagatgga aaacgggatg
     3601 tggggaaact cgcccgtagg tgtgaagtga ggggaaaacc ggtgataaag taaaaagctt
     3661 acctaacact atagtaacaa agaaagccca attatcaatt ttagtgctga ggaattggtc
     3721 tctttaataa atttccttaa cgttgtaaat ccgcattttc ctgacggtac ccccaaacta
     3781 agatttcata gatagatttt ctgataactt taaaaaacac gaatagcgga aaggatgtct
     3841 tcattcgcta aagagtaata aactactttt ccatcttgac gtgattttgc cattccatgt
     3901 ttttttaaaa aacgtaaatg gtgtgacgtt gtagcgacac tagcagtaag tatttctgct
     3961 aaatcacaga cgcacatttc cttataggtt tctaaagcat agaaaatttt aattctagaa
     4021 aaatcgctga aacatttccc taacacaagt aagtttgaaa aatctttttg atccaattta
     4081 ttttttattc catttacttt ttctttatgt acgacagtca cttggcaaac ttttttcatt
     4141 catctcacct cacattcaaa cgattatttg aatgaagtat acctatttga tcactctttt
     4201 gtcaatctac cttaattcca atacactatt ataagcaatt tcatgtattg aagtaaactt
     4261 acttgcaagt gcgtttttga ttccaagtta aaaacatatt attattctac ataatttcct
     4321 aatcaaataa aactcctttg aaaattgttc taaaggagtt ttattatttt gtatttctac
     4381 taaaaatagc gtacaatgta attactaagt aattacgaaa tgtaaaataa tggaggaaat
     4441 aatatgatta caaaaacaag aaaacaagga aattctatta tgttaacggt ccctaaagaa
     4501 ttcgatgtgc ccaatggtgt tgaagtagaa gctaagcttg tagagaatgg tatcctttat
     4561 gagtttgtag agcctaaaaa agaatttttt gattttagtg aagatatttt ggctgatatt
     4621 ctttcagaag gttatagcaa acaagagatc ttaaaagaat ttagaaatag aaaaaatgcg
     4681 ttaacttctg catttaaatc tattgctgaa gatactgttg caaattctaa accgatgaca
     4741 aaagaggagt tggcagcaga aattggctta tgagattcta ccttctaaac acgtaataaa
     4801 gtatctaaaa aaattaaaag agaaaacatt aaaagaacaa tttttaacga taatttatga
     4861 tgagatagca gttcgtcctc attcgggaga acaaaaaacg ggtgatttat caggaatttg
     4921 ggctattgga ttcaagtatg caggtacgac atatcgcgtt gcatatgaaa ttaaagataa
     4981 tgcagtgata cctatcttac tatgtggaac gcatgaaaat ttttatgaac aattaaaaaa
     5041 gattaggtaa gaacccaatg tttcaacttg agaggataga cgacacttta aaagataatt
     5101 tttaacagct gagagttgta ataccctata attttaaacc actaacctat atatttatga
     5161 aaaatagaaa aaattaaaac gcattttaag cctctttcaa attaaaagct tcatccttat
     5221 gcacaaagag ggcttaaaat gcgttatttt atattgtttt ttagtaataa taaaaaaatc
     5281 actctaattc tcccataaat attatagatt cctaaagatt aaaatagatt attccctatt
     5341 gtcttttaag tagcggtaga gtgttgcctt gctaatacca gtcatttccg taatttcttt
     5401 tacgctataa acctgacttt tatacaattt tactgctcga tcaacatctt tctggttcac
     5461 tcgtggacgg ccacctttac gacctctcgc acgtgcactt ttcaaacctt ctttggtacg
     5521 ttcaacaatt aaatcacgtt cgaactgact aaatgcttgg aagacagtca tcattaagcg
     5581 tccgtgaggc gtgctggtat cgaagttttc tttcaagctc accagcttaa catggtgttc
     5641 ttcaaaatag gtaacaaggt caatcaaatc ctttgtacta cgacccaatc gagaaaaact
     5701 ttctacaatt accgtatctc ccggacgaat tttatctttt aaacgattta gttggggacg
     5761 aaccgctttg gttcccgtca ttttctcagt taggatttca ttacaatttt gttcattgag
     5821 taagtctaat tgacgattta gttcttgtga acgtgtactc actcgggcat aaccataaat
     5881 gtatccactc atgcttttct tcctttcgta ctttaattct ggcttgattg tatcataaaa
     5941 gggagttata gatacatagt taatgcaaca agttatgata caaaatatga cctcttttat
     6001 taaataaaag aggtcatttc attttgtctc ataatcgatt gtttttgata cgagtgtgta
     6061 tacgaaataa acaaaaaaat cttaagtaaa tttctagata cctaatttaa tcctaactag
     6121 tgtatcctaa agttaataac gagtttatca caatgaaaat tataaaatgc tcccatatta
     6181 gatataacat gcaatatttg aattagaact acaaaattta ttcgttttat aaagtaaata
     6241 atgttgagat actaaggaat agaatattat aaggaattga gctgaatgta ttggataaaa
     6301 aaagaataac tttattagag atggactata aaaatgctcg taattttctt ctgaagtctg
     6361 aaagctattt gaatgctcag ctaccagaat atattagttt tgacgaaact ttaaaatctg
     6421 ctaaaaaagt tttaacttcg ccgtttgtat aattaagcga gacaaataaa aaagc
//
LOCUS       DAZABD010000210         4429 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.guided.128, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000210 DAZABD010000000
VERSION     DAZABD010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.guided.128"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            241..1209
                     /locus_tag="ACHALG_003036"
     CDS             241..1209
                     /locus_tag="ACHALG_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFN2588311.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1202..2233
                     /gene="vanA"
                     /locus_tag="ACHALG_003037"
     CDS             1202..2233
                     /gene="vanA"
                     /locus_tag="ACHALG_003037"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFN2588312.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2239..2847
                     /locus_tag="ACHALG_003038"
     CDS             2239..2847
                     /locus_tag="ACHALG_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HFN2588313.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
     gene            3275..4186
                     /gene="vanY"
                     /locus_tag="ACHALG_003039"
     CDS             3275..4186
                     /gene="vanY"
                     /locus_tag="ACHALG_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HFN2588314.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYELWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
BASE COUNT         1400 a          777 c         1016 g         1236 t
ORIGIN      
        1 cttatttgat agtagattta aaactttgca acagaaccat tttttaggaa aatctcaagg
       61 ttatctttac tttttcttag gaaattaaca atttaatatt aagaaacggc tcgttcttac
      121 acggtagact taataccgta agaacgagcc gttttcgttc ttcagagaaa gatttgacaa
      181 gattaccatt ggcatccccg ttttatttgg tgcctttcac agaaagggtt ggtcttaatt
      241 atgaataaca tcggcattac tgtttatgga tgtgagcagg atgaggcaga tgcattccat
      301 gctctttcgc ctcgctttgg cgttatggca acgataatta acgccaacgt gtcggaatcc
      361 aacgccaaat ccgcgccttt caatcaatgt atcagtgtgg gacataaatc agagatttcc
      421 gcctctattc ttcttgcgct gaagagagcc ggtgtgaaat atatttctac ccgaagcatc
      481 ggctgcaatc atatagatac aactgctgct aagagaatgg gcatcactgt cgacaatgtg
      541 gcgtactcgc cggatagcgt tgccgattat actatgatgc taattcttat ggcagtacgc
      601 aacgtaaaat cgattgtgcg ctctgtggaa aaacatgatt tcaggttgga cagcgaccgt
      661 ggcaaggtac tcagcgacat gacagttggt gtggtgggaa cgggccagat aggcaaagcg
      721 gttattgaac ggctgcgagg atttggatgt aaagtgttgg cttatagtcg cagccgaagt
      781 atagaggtaa actatgtacc gtttgatgag ttgctgcaaa atagcgatat cgttacgctt
      841 catgtgccgc tcaatacgga tacgcactat attatcagcc acgaacaaat acagagaatg
      901 aagcaaggag catttcttat caatactggg cgcggtccac ttgtagatac ctatgagttg
      961 gttaaagcat tagaaaacgg gaaactgggc ggtgccgcat tggatgtatt ggaaggagag
     1021 gaagagtttt tctactctga ttgcacccaa aaaccaattg ataatcaatt tttacttaaa
     1081 cttcaaagaa tgcctaacgt gataatcaca ccgcatacgg cctattatac cgagcaagcg
     1141 ttgcgtgata ccgttgaaaa aaccattaaa aactgtttgg attttgaaag gagacaggag
     1201 catgaataga ataaaagttg caatactgtt tgggggttgc tcagaggagc atgacgtatc
     1261 ggtaaaatct gcaatagaga tagccgctaa cattaataaa gaaaaatacg agccgttata
     1321 cattggaatt acgaaatctg gtgtatggaa aatgtgcgaa aaaccttgcg cggaatggga
     1381 aaacgacaat tgctattcag ctgtactctc gccggataaa aaaatgcacg gattacttgt
     1441 taaaaagaac catgaatatg aaatcaacca tgttgatgta gcattttcag ctttgcatgg
     1501 caagtcaggt gaagatggat ccatacaagg tctgtttgaa ttgtccggta tcccttttgt
     1561 aggctgcgat attcaaagct cagcaatttg tatggacaaa tcgttgacat acatcgttgc
     1621 gaaaaatgct gggatagcta ctcccgcctt ttgggttatt aataaagatg ataggccggt
     1681 ggcagctacg tttacctatc ctgtttttgt taagccggcg cgttcaggct catccttcgg
     1741 tgtgaaaaaa gtcaatagcg cggacgaatt ggactacgca attgaatcgg caagacaata
     1801 tgacagcaaa atcttaattg agcaggctgt ttcgggctgt gaggtcggtt gtgcggtatt
     1861 gggaaacagt gccgcgttag ctgttggcga ggtggaccaa atcaggctgc agtacggaat
     1921 ctttcgtatt catcaggaag tcgagccgga aaaaggctct gaaaacgcag ttataaccgt
     1981 tcccgcagac ctttcagcag aggagcgagg acggatacag gaaacggcaa aaaaaatata
     2041 taaagcgctc ggctgtagag gtctagcccg tgtggatatg tttttacaag ataacggccg
     2101 cattgtactg aacgaagtca atactctgcc cggtttcacg tcatacagtc gttatccccg
     2161 tatgatggcc gctgcaggta ttgcacttcc cgaactgatt gaccgcttga tcgtattagc
     2221 gttaaagggg tgataagcat ggaaatagga tttacttttt tagatgaaat agtacacggt
     2281 gttcgttggg acgctaaata tgccacttgg gataatttca ccggaaaacc ggttgacggt
     2341 tatgaagtaa atcgcattgt agggacatac gagttggctg aatcgctttt gaaggcaaaa
     2401 gaactggctg ctacccaagg gtacggattg cttctatggg acggttaccg tcctaatcgt
     2461 gctgtaaact gttttatgca atgggctgca cagccggaaa ataacctgac aaaggaaagt
     2521 tattatccca atattgaccg aactgagatg atttcaaaag gatacgtggc ttcaaaatca
     2581 agccatagcc gcggcagtgc cattgatctt acgctttatc gattagacac gggtgagctt
     2641 gtaccaatgg ggagccgatt tgattttatg gatgaacgct ctcatcatgc ggcaaatgga
     2701 atatcatgca atgaagcgca aaatcgcaga cgtttgcgct ccatcatgga aaacagtggg
     2761 tttgaagcat atagcctcga atggtggcac tatgtattaa gagacgaacc ataccccaat
     2821 agctattttg atttccccgt taaataaact tttaaccgtt gcacggacaa actatataag
     2881 ctaactcttt cggcaggaaa cccgacgtat gtaactggtt cttagggaat ttatatatag
     2941 tagatagtat tgaagatgta aggcagagcg atattgcggt cattatctgc gtgcgctgcg
     3001 gcaagatagc ctgataataa gactgatcgc atagaggggt ggtatttcac accgcccatt
     3061 gtcaacaggc agttcagcct cgttaaattc agcatgggta tcacttatga aaattcatct
     3121 acattggtga taatagtaaa tccagtaggg cgaaataatt gactgtaatt tacggggcaa
     3181 aacggcacaa tctcaaacga gattgtgccg tttaagggga agattctaga aatatttcat
     3241 acttccaact atatagttaa ggaggagact gaaaatgaag aagttgtttt ttttattgtt
     3301 attgttattc ttaatatact taggttatga ctacgttaat gaagcactgt tttctcagga
     3361 aaaagtcgaa tttcaaaatt atgatcaaaa tcccaaagaa catttagaaa atagtgggac
     3421 ttctgaaaat acccaagaga aaacaattac agaagaacag gtttatcaag gaaatctgct
     3481 attaatcaat agtaaatatc ctgttcgcca agaaagtgtg aagtcagata tcgtgaattt
     3541 atctaaacat gacgaattaa taaatggata cgggttgctt gatagtaata tttatatgtc
     3601 aaaagaaata gcacaaaaat tttcagagat ggtcaatgat gctgtaaagg gtggcgttag
     3661 tcattttatt attaatagtg gctatcgaga ctttgatgag caaagtgtgc tttaccaaga
     3721 aatgggggct gagtatgcct taccagcagg ttatagtgag cataattcag gtttatcact
     3781 agatgtagga tcaagcttga cgaaaatgga acgagcccct gaaggaaagt ggatagaaga
     3841 aaatgcttgg aaatacgggt tcattttacg ttatccagag gacaaaacag agttaacagg
     3901 aattcaatat gaactatggc atattcgcta tgttggttta ccacatagtg cgattatgaa
     3961 agaaaagaat ttcgttctcg aggaatatat ggattaccta aaagaagaaa aaaccatttc
     4021 tgttagtgta aatggggaaa aatatgagat cttttattat cctgttacta aaaataccac
     4081 cattcatgtg ccgactaatc ttcgttatga gatatcagga aacaatatag acggtgtaat
     4141 tgtgacagtg tttcccggat caacacatac taattcaagg aggtaaggat ggcggaatga
     4201 aaccaacgaa attaatgaac agcattattg tactagcact tttggggtaa cgttagcttt
     4261 ttaatttaaa acccacgtta actaggacat tgctatacta atgatacaac ttaaacaaaa
     4321 gaattagagg aaattatatt gggaaaaata ttatctagag gattgctagc tttatattta
     4381 gtgacactaa tctggttagt gttattcaaa ttacaataca atattttat
//
LOCUS       DAZABD010000211         3169 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.guided.149, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000211 DAZABD010000000
VERSION     DAZABD010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3169
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.guided.149"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..973
                     /gene="vanA"
                     /locus_tag="ACHALG_003040"
     CDS             <1..973
                     /gene="vanA"
                     /locus_tag="ACHALG_003040"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase"
                     /protein_id="HFN2588315.1"
                     /translation="VKSAIEIAANINKKKYEPLYIGITKSGVWKMCEKPCAEWENDNC
                     YSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKSGEDGSIQGLFELSGIPFVGC
                     DIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPVAATFTYPVFVKPARSGSSFG
                     VKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCAVLGNSAALAVGEVDQIRLQY
                     GIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETAKKIYKALGCRGLARVDMFLQ
                     DNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELIDRLIVLALKG"
     gene            979..1587
                     /locus_tag="ACHALG_003041"
     CDS             979..1587
                     /locus_tag="ACHALG_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HFN2588316.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
     gene            2015..2926
                     /gene="vanY"
                     /locus_tag="ACHALG_003042"
     CDS             2015..2926
                     /gene="vanY"
                     /locus_tag="ACHALG_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HFN2588317.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYELWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
BASE COUNT         1027 a          539 c          719 g          884 t
ORIGIN      
        1 ggtaaaatct gcaatagaga tagccgctaa cattaataaa aaaaaatacg agccgttata
       61 cattggaatt acgaaatctg gtgtatggaa aatgtgcgaa aaaccttgcg cggaatggga
      121 aaacgacaat tgctattcag ctgtactctc gccggataaa aaaatgcacg gattacttgt
      181 taaaaagaac catgaatatg aaatcaacca tgttgatgta gcattttcag ctttgcatgg
      241 caagtcaggt gaagatggat ccatacaagg tctgtttgaa ttgtccggta tcccttttgt
      301 aggctgcgat attcaaagct cagcaatttg tatggacaaa tcgttgacat acatcgttgc
      361 gaaaaatgct gggatagcta ctcccgcctt ttgggttatt aataaagatg ataggccggt
      421 ggcagctacg tttacctatc ctgtttttgt taagccggcg cgttcaggct catccttcgg
      481 tgtgaaaaaa gtcaatagcg cggacgaatt ggactacgca attgaatcgg caagacaata
      541 tgacagcaaa atcttaattg agcaggctgt ttcgggctgt gaggtcggtt gtgcggtatt
      601 gggaaacagt gccgcgttag ctgttggcga ggtggaccaa atcaggctgc agtacggaat
      661 ctttcgtatt catcaggaag tcgagccgga aaaaggctct gaaaacgcag ttataaccgt
      721 tcccgcagac ctttcagcag aggagcgagg acggatacag gaaacggcaa aaaaaatata
      781 taaagcgctc ggctgtagag gtctagcccg tgtggatatg tttttacaag ataacggccg
      841 cattgtactg aacgaagtca atactctgcc cggtttcacg tcatacagtc gttatccccg
      901 tatgatggcc gctgcaggta ttgcacttcc cgaactgatt gaccgcttga tcgtattagc
      961 gttaaagggg tgataagcat ggaaatagga tttacttttt tagatgaaat agtacacggt
     1021 gttcgttggg acgctaaata tgccacttgg gataatttca ccggaaaacc ggttgacggt
     1081 tatgaagtaa atcgcattgt agggacatac gagttggctg aatcgctttt gaaggcaaaa
     1141 gaactggctg ctacccaagg gtacggattg cttctatggg acggttaccg tcctaatcgt
     1201 gctgtaaact gttttatgca atgggctgca cagccggaaa ataacctgac aaaggaaagt
     1261 tattatccca atattgaccg aactgagatg atttcaaaag gatacgtggc ttcaaaatca
     1321 agccatagcc gcggcagtgc cattgatctt acgctttatc gattagacac gggtgagctt
     1381 gtaccaatgg ggagccgatt tgattttatg gatgaacgct ctcatcatgc ggcaaatgga
     1441 atatcatgca atgaagcgca aaatcgcaga cgtttgcgct ccatcatgga aaacagtggg
     1501 tttgaagcat atagcctcga atggtggcac tatgtattaa gagacgaacc ataccccaat
     1561 agctattttg atttccccgt taaataaact tttaaccgtt gcacggacaa actatataag
     1621 ctaactcttt cggcaggaaa cccgacgtat gtaactggtt cttagggaat ttatatatag
     1681 tagatagtat tgaagatgta aggcagagcg atattgcggt cattatctgc gtgcgctgcg
     1741 gcaagatagc ctgataataa gactgatcgc atagaggggt ggtatttcac accgcccatt
     1801 gtcaacaggc agttcagcct cgttaaattc agcatgggta tcacttatga aaattcatct
     1861 acattggtga taatagtaaa tccagtaggg cgaaataatt gactgtaatt tacggggcaa
     1921 aacggcacaa tctcaaacga gattgtgccg tttaagggga agattctaga aatatttcat
     1981 acttccaact atatagttaa ggaggagact gaaaatgaag aagttgtttt ttttattgtt
     2041 attgttattc ttaatatact taggttatga ctacgttaat gaagcactgt tttctcagga
     2101 aaaagtcgaa tttcaaaatt atgatcaaaa tcccaaagaa catttagaaa atagtgggac
     2161 ttctgaaaat acccaagaga aaacaattac agaagaacag gtttatcaag gaaatctgct
     2221 attaatcaat agtaaatatc ctgttcgcca agaaagtgtg aagtcagata tcgtgaattt
     2281 atctaaacat gacgaattaa taaatggata cgggttgctt gatagtaata tttatatgtc
     2341 aaaagaaata gcacaaaaat tttcagagat ggtcaatgat gctgtaaagg gtggcgttag
     2401 tcattttatt attaatagtg gctatcgaga ctttgatgag caaagtgtgc tttaccaaga
     2461 aatgggggct gagtatgcct taccagcagg ttatagtgag cataattcag gtttatcact
     2521 agatgtagga tcaagcttga cgaaaatgga acgagcccct gaaggaaagt ggatagaaga
     2581 aaatgcttgg aaatacgggt tcattttacg ttatccagag gacaaaacag agttaacagg
     2641 aattcaatat gaactatggc atattcgcta tgttggttta ccacatagtg cgattatgaa
     2701 agaaaagaat ttcgttctcg aggaatatat ggattaccta aaagaagaaa aaaccatttc
     2761 tgttagtgta aatggggaaa aatatgagat cttttattat cctgttacta aaaataccac
     2821 cattcatgtg ccgactaatc ttcgttatga gatatcagga aacaatatag acggtgtaat
     2881 tgtgacagtg tttcccggat caacacatac taattcaagg aggtaaggat ggcggaatga
     2941 aaccaacgaa attaatgaac agcattattg tactagcact tttggggtaa cgttagcttt
     3001 ttaatttaaa acccacgtta actaggacat tgctatacta atgatacaac ttaaacaaaa
     3061 gaattagagg aaattatatt gggaaaaata ttatctagag gattgctagc tttatattta
     3121 gtgacactaa tctggttagt gttattcaaa ttacaataca atattttat
//
LOCUS       DAZABD010000212         2810 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.guided.154, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000212 DAZABD010000000
VERSION     DAZABD010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.guided.154"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            57..110
                     /locus_tag="ACHALG_003043"
                     /pseudo
     CDS             57..110
                     /locus_tag="ACHALG_003043"
                     /inference="COORDINATES: protein motif:HMM:NF019662.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance efflux system leader
                     peptide"
     gene            144..1520
                     /gene="tet(L)"
                     /locus_tag="ACHALG_003044"
     CDS             144..1520
                     /gene="tet(L)"
                     /locus_tag="ACHALG_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001574277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HFN2588318.1"
                     /translation="MNTSYSQSNLRHNQILIWLCILSFFSVLNEMVLNVSLPDIANDF
                     NKPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSF
                     FSLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGG
                     MIAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFT
                     TSYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFV
                     SMVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLS
                     VSFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFT
                     SFLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNV
                     YKHSQRDF"
     gene            2084..>2810
                     /gene="mobV"
                     /locus_tag="ACHALG_003045"
     CDS             2084..>2810
                     /gene="mobV"
                     /locus_tag="ACHALG_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="HFN2588319.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYS
                     DKVKSDLEVPAKRFCCKVLNLLSN"
BASE COUNT          897 a          420 c          571 g          922 t
ORIGIN      
        1 tattttttaa taaagatttg aattaaagtg taaaggagga attaattatg aagtgaaatg
       61 aatgtaacag ggttcaatta aaagagggaa gcgtatcatt aaccctataa actacgtctg
      121 ccctcattat tggagggtga aatgtgaata catcctattc acaatcgaat ttacgacaca
      181 accaaatttt aatttggctt tgcattttat ctttttttag cgtattaaat gaaatggttt
      241 tgaacgtctc attacctgat attgcaaatg attttaataa accacctgcg agtacaaact
      301 gggtgaacac agcctttatg ttaacctttt ccattggaac agctgtatat ggaaagctat
      361 ctgatcaatt aggcatcaaa aggttactcc tatttggaat tataataaat tgtttcgggt
      421 cggtaattgg gtttgttggc cattctttct tttccttact tattatggct cgttttattc
      481 aaggggctgg tgcagctgca tttccagcac tcgtaatggt tgtagttgcg cgctatattc
      541 caaaggaaaa taggggtaaa gcatttggtc ttattggatc gatagtagcc atgggagaag
      601 gagtcggtcc agcgattggt ggaatgatag cccattatat tcattggtcc tatcttctac
      661 tcattcctat gataacaatt atcactgttc cgtttcttat gaaattatta aagaaagaag
      721 taaggataaa aggtcatttt gatatcaaag gaattatact aatgtctgta ggcattgtat
      781 tttttatgtt gtttacaaca tcatatagca tttcttttct tatcgttagc gtgctgtcat
      841 tcctgatatt tgtaaaacat atcaggaaag taacagatcc ttttgttgat cccggattag
      901 ggaaaaatat accttttatg attggagttc tttgtggggg aattatattt ggaacagtag
      961 cagggtttgt ctctatggtt ccttatatga tgaaagatgt tcaccagcta agtactgccg
     1021 aaatcggaag tgtaattatt ttccctggaa caatgagtgt cattattttc ggctacattg
     1081 gtgggatact tgttgataga agaggtcctt tatacgtgtt aaacatcgga gttacatttc
     1141 tttctgttag ctttttaact gcttcctttc ttttagaaac aacatcatgg ttcatgacaa
     1201 ttataatcgt atttgtttta ggtgggcttt cgttcaccaa aacagttata tcaacaattg
     1261 tttcaagtag cttgaaacag caggaagctg gtgctggaat gagtttgctt aactttacca
     1321 gctttttatc agagggaaca ggtattgcaa ttgtaggtgg tttattatcc atacccttac
     1381 ttgatcaaag gttgttacct atggaagttg atcagtcaac ttatctgtat agtaatttgt
     1441 tattactttt ttcaggaatc attgtcatta gttggctggt taccttgaat gtatataaac
     1501 attctcaaag ggatttctaa atcgttaagg gatcaacttt gggagagagt tcaaaattga
     1561 tccttttttt ataacaggaa ttcaaatctt tttgttccat taaagggcgc gattgctgaa
     1621 taaaagatac gagagacctc tcttgtatct tttttatttt gagtggtttt gtccgttaca
     1681 ctagaaaacc gaaagacaat aaaaatttta ttcttgctga gtctggcttt cggtaagcta
     1741 gacaaaacgg acaaaataaa aattggcaag ggtttaaagg tggagatttt ttgagtgatc
     1801 ttctcaaaaa atactacctg tcccttgctg atttttaaac gagcacgaga gcaaaacccc
     1861 cctttgctga ggtggcagag ggcaggtttt tttgtttctt ttttctcgta aaaaaaagaa
     1921 aggtcttaaa ggttttatgg ttttggtcgg cactgccgac agcctcgcag agcacacact
     1981 ttatgaatat aaagtatagt gtgttatact ttacttggaa gtggttgccg gaaagagcga
     2041 aaatgcctca catttgtgcc acctaaaaag gagcgattta catatgagtt atgcagtttg
     2101 tagaatgcaa aaagtgaaat cagctggact aaaaggcatg caatttcata atcaaagaga
     2161 gcgaaaaagt agaacgaatg atgatattga ccatgagcga acacgtgaaa attatgattt
     2221 gaaaaatgat aaaaatattg attacaacga acgtgtcaaa gaaattattg aatcacaaaa
     2281 aacaggtaca agaaaaacga ggaaagatgc tgttcttgta aatgagttgc tagtaacatc
     2341 tgaccgagat ttttttgagc aactggatcc aggagaacaa aaacgatttt ttgaggaaag
     2401 ttataaatta ttttccgaac gatatggcaa gcaaaatatt gcttatgcaa cagttcataa
     2461 tgatgagcaa acccctcaca tgcatttagg tgttgtgcct atgcgtgatg gaaaactgca
     2521 aggaaaaaat gtgtttaatc gtcaagaact gttatggcta caagataaat tccccgagca
     2581 tatgaaaaaa cagggttttg agttgaagcg tggtgaacgt ggctctgacc gtaaacatat
     2641 tgagacagct aaatttaaaa aacaaacttt ggaaaaagag attgattttc tagaaaaaaa
     2701 tttagcagtt aaaaaagatg aatggactgc ttatagcgat aaagttaaat cagatttaga
     2761 agtaccagcg aaacggttct gttgcaaagt tttaaatcta ctatcaaata
//
LOCUS       DAZABD010000213         2566 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.guided.156, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000213 DAZABD010000000
VERSION     DAZABD010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.guided.156"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(39..341)
                     /locus_tag="ACHALG_003046"
                     /pseudo
     CDS             complement(39..341)
                     /locus_tag="ACHALG_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
     gene            434..783
                     /locus_tag="ACHALG_003047"
                     /pseudo
     CDS             434..783
                     /locus_tag="ACHALG_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(1055..1345)
                     /locus_tag="ACHALG_003048"
     CDS             complement(1055..1345)
                     /locus_tag="ACHALG_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2588320.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1553..2245)
                     /locus_tag="ACHALG_003049"
     CDS             complement(1553..2245)
                     /locus_tag="ACHALG_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFN2588321.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          759 a          472 c          357 g          978 t
ORIGIN      
        1 atggattttt tcatttacac aaaatatttt atactctcaa catacttgca aagtaatact
       61 acaattccta acataatcgt agaatttaat aatttccata gcataaagtg tttagaaaag
      121 aacattaaga atagttctat aataattctt gaactccatg tcttataatg ttctatcgaa
      181 gcacccaaat agccaagttc atctaatcta cttgcataaa ctacgtcatc cgctctcata
      241 gtcggcatat acatattaaa gagtaaaaag ataccccaaa tccctattag taacaagatc
      301 agttctttgt tattcgttat ttttctatta tgtttgttca tttttatact ccttcttata
      361 tttcctaata gagtataaca tagctttaag cattacttaa agataactta aaagttatct
      421 tttaaatact taattgttaa ataccttatt tgatgcaaaa gaaatctacg atagtcaaca
      481 aaaaaatttt cattatatcc agaaaaattt ttcttagtta aggacctatg gattgcagta
      541 atgcagaatt cgtcgaataa actgccaaag acatacaatc atattgcttt taaaattgac
      601 gaacaagata tagattcgtt tgtctcaaaa atccaaatgt taggtttaac tgtcgaacca
      661 ggacgttctc gtgtgaaagg cgaggcttct tcaatttact tttacgacta tgataatcac
      721 ctcttcgagt tacataccgg tactctccag gaacgtttaa aatcctacaa ttccactacc
      781 tgagactagt aatgactagt ctctttttat ggcctctgat ccccccattg agcgaatact
      841 aatagggctt cgaattgatc tgttcaatct ttaagcaata tccctcaaat gagtttggaa
      901 agtttagtga ttcatcacaa atacttaaat acatgtagtt atatatagat tgaggttctt
      961 gtgagcgtat gctcgtgtgg gttcaaatcc catcgtctgc atccatcttt ccaaaacaag
     1021 cggctaggac atttttgtct cagccgcttg ttttctagtt tattctttca tcttgtcgga
     1081 ccttttttct tgggtataga cttttctcat attctaccac aattttgggt ttccctaaca
     1141 catgatagga tctcacaaca aattgctttc tattattata ataacctgta acgactaact
     1201 tcatcccttc gtctacttca gctaggaagt tcaaactatg ggaagcaatc aaacaattga
     1261 ccccatttaa actgaaacgg atcaacggtc tggcactaac ttgcaaagta cggatcttat
     1321 ctactgttcc atattttttc tccataacct tcacctcaat accattatac gaacatacat
     1381 tccctttgta aagaagaaac tctgttttat ctttttgact ttttgtctat tacctttctt
     1441 aaaatcatat tggcacttcc tgcgaagatg aaggacatgt actaaaaaaa agaggcagca
     1501 gtatagatta ctgttgcttc tttactaatt taggtttact tgttaagaaa gattatttac
     1561 gcaaatgttc ttcaacaatt tgctgagatt tatcagctgt atcttccact tcatcttttg
     1621 ctttttctaa cttttgaata tattgtttca agcttgagtt ttcagcagtt gcttcgtcta
     1681 atttcttttg tgtatctgtt aactgagttt ctacatttgt tttttcttgt gtcagtgttg
     1741 cgatttgttg attcaaacca tttacagtat tttgtaattc tgaaagtttt gcttgtgctt
     1801 cttgtagtct tgctgtccaa tctgcattca gatgattgat ttcatcattt ttttggttga
     1861 tctcatctag tttgacattg atttcatttt gtttgctttg tagttgtgca tccttgctga
     1921 ctaattgact ttctaagttt tgtttttctg ctgccgattg atttttttgg ttttctaatt
     1981 catttacttt tgtttggtat tcttggacat ctgcctttga atcttgcaat gtctgttctt
     2041 tttgtgctag tcgagcggta agatctgtaa tcgcttgctc tttatctgtc aatgtatgat
     2101 caagctgtgt cagattctca ttgattgtat caatatctgt ctctccaccc cacaaaataa
     2161 tttcatttgc gtaccgcgtg cctgtgtagc caattagaca tagaaatgca acgacaatcc
     2221 caccagtaat aaatttcttt ttcatttgca tagctcctta atccaatact tttacatgct
     2281 aaaagtaagt gatctcaaac acaatgtcaa gcgtttttaa attacatatg taattttaat
     2341 ataaatacta tcaaatactc atttaatcct ccttttttat ctatttaaaa aatctttttt
     2401 atagagaaaa atgcaggtta atactttctt aaataaatat ttatggtgaa caaaaacaaa
     2461 aaagcaagtt ttattaaacg taaccctttt catttcatag attactgtat ttattacaaa
     2521 aaacgcggat ttgtcataaa ttgtcatgct tcttttacct atgaaa
//
LOCUS       DAZABD010000214         2081 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.guided.165, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000214 DAZABD010000000
VERSION     DAZABD010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.guided.165"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            72..1991
                     /gene="tet(M)"
                     /locus_tag="ACHALG_003050"
     CDS             72..1991
                     /gene="tet(M)"
                     /locus_tag="ACHALG_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HFN2588322.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYTSGKLLEALELEQEESIRFHNCSLFPVYH
                     GSAKNNIGIDNLIEVITNKFYSSTHRGQSELCGKVFKIEYSEKRQRLAYIRLYSGVLH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIEIKEPTVIYMERPLKKAEYTIHIEVPPNPFWASIGLSVA
                     QLPLGSGVQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
BASE COUNT          712 a          316 c          431 g          622 t
ORIGIN      
        1 atcccagtga taagagtatt tatcactggg atttttatgc ccttttgggc ttttgaatgg
       61 aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat gcgggaaaaa
      121 ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta ggaagcgtgg
      181 acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga attacaattc
      241 agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata gacacgccag
      301 gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat ggggcaattc
      361 tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt catgcactta
      421 ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat ggaattgatt
      481 tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta atcaaacaga
      541 aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa caatgggata
      601 cggtaataga gggaaacgat gaccttttag agaaatatac gtctgggaaa ttattggaag
      661 cattagaact cgaacaagag gaaagcataa gatttcataa ttgttccctg ttccctgttt
      721 atcacggaag tgcaaaaaac aatataggga ttgataacct tatagaagtg attacgaata
      781 aattttattc atcaacacat cgaggtcagt ctgaactttg cggaaaagtt ttcaaaattg
      841 agtattcgga aaaaagacag cgtcttgcat atatacgtct ttatagtggc gtactgcatt
      901 tgcgagattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa atgtatactt
      961 caataaatgg tgaattatgt aaaatcgata aggcttattc cggggaaatt gttattttgc
     1021 aaaatgagtt tttgaagtta aatagtgttc ttggagatac aaaactattg ccacagagaa
     1081 aaaagattga aaatccgcac cctctactac aaacaactgt tgaaccgagt aaacctgaac
     1141 agagagaaat gttgcttgat gcccttttgg aaatctcaga tagtgatccg cttctacgat
     1201 attacgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa gtacaaatgg
     1261 aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaaata aaagagccta
     1321 cagtcattta tatggaaaga ccgttaaaaa aagcagagta taccattcac atcgaagttc
     1381 caccgaatcc tttctgggct tccattggtc tatctgtagc acagcttcca ttagggagcg
     1441 gagtacagta tgagagctcg gtttctcttg gatacttaaa tcaatcgttt caaaatgcag
     1501 ttatggaggg gatacgctat ggctgtgaac aaggattgta tggttggaat gtgacggact
     1561 gtaaaatctg ttttaagtat ggcttatact atagccctgt tagtacccca gcagattttc
     1621 ggatgcttgc tcctattgta ttggaacaag tcttaaaaaa agctggaaca gaattgttag
     1681 agccatatct tagttttaaa atttatgcgc cacaggaata tctttcacga gcatacaacg
     1741 atgctcctaa atattgtgcg aacatcgtag acactcaatt gaaaaataat gaggtcattc
     1801 ttagtggaga aatccctgct cggtgtattc aagaatatcg tagtgattta actttcttta
     1861 caaatggacg tagtgtttgt ttaacagagt taaaagggta ccatgttact accggtgaac
     1921 ctgtttgcca gccccgtcgt ccaaatagtc ggatagataa agtacgatat atgttcaata
     1981 aaataactta gtgtatttta tgttgttata taaatatggt ttcttgttaa ataagatgaa
     2041 atatttttta ataaagattt gaattaaagt gtaaaggagg a
//
LOCUS       DAZABD010000215         1504 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.guided.179, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000215 DAZABD010000000
VERSION     DAZABD010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.guided.179"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            262..345
                     /locus_tag="ACHALG_003051"
                     /pseudo
     CDS             262..345
                     /locus_tag="ACHALG_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502600.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MLS leader peptide"
     gene            394..477
                     /locus_tag="ACHALG_003052"
     CDS             394..477
                     /locus_tag="ACHALG_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HFN2588323.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            602..1339
                     /gene="erm(B)"
                     /locus_tag="ACHALG_003053"
     CDS             602..1339
                     /gene="erm(B)"
                     /locus_tag="ACHALG_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFN2588324.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          551 a          224 c          245 g          484 t
ORIGIN      
        1 atttgtatcg ataagaaata gatttaaaaa tttcgctgtt attttgtaca tttaacttga
       61 cggtgacatc tctctattgt gagttattag tggtacagtt ttcaaccgtt ttaattataa
      121 aaaagtggtg catttttaaa ttggcacaaa caggtaacgg ttattgcagg tgtatttctt
      181 atctatgggt ttaacatgga ttttatcatt aaaatcatga gtattgtccg agagtgattg
      241 gtcttgcgta tggttaaccc taaagttatg gaaataagac ttagaagcaa acttaagagt
      301 gtgttgatag tgcattatct taaaattttg tataatagga attgaagtta aattagatgc
      361 taaaaatttg taattaagaa ggagggattc gtcatgttgg tattccaaat gcgttatgta
      421 gataaaacat ctactgtttt gaaacagact aaaaacagtg attacgcaga taaataaata
      481 cgttagatta attcctacca gtgactaatc ttatgacttt ttaaacagat aactaaaatt
      541 acaaacaaat cgtttaactt ctgtatttgt ttatagatgt aatcacttca ggagtgataa
      601 catgaacaaa aatataaaat attctcaaaa ctttttaacg agtgaaaaag tactcaacca
      661 aataataaaa caattgaatt taaaagaaac cgataccgtt tacgaaattg gaacaggtaa
      721 agggcattta acgacgaaac tggctaaaat aagtaaacag gtaacgtcta ttgaattaga
      781 cagtcatcta ttcaacttat cgtcagaaaa attaaaactg aatactcgtg tcactttaat
      841 tcaccaagat attctacagt ttcaattccc taacaaacag aggtataaaa ttgttgggaa
      901 tattccttac catttaagca cacaaattat taaaaaagtg gtttttgaaa gccgtgcgtc
      961 tgacatctat ctgattgttg aagaaggatt ctacaagcgt accttggata ttcaccgaac
     1021 actagggttg ctcttgcaca ctcaagtctc gattcagcaa ttgcttaagc tgccagcgga
     1081 atgctttcat cctaaaccaa aagtaaacag tgtcttaata aaacttaccc gccataccac
     1141 agatgttcca gataaatatt ggaagctata tacgtacttt gtttcaaaat gggtcaatcg
     1201 agaatatcgt caactgttta ctaaaaatca gtttcatcaa gcaatgaaac acgccaaagt
     1261 aaacaattta agtaccgtta cttatgagca agtattgtct atttttaata gttatctatt
     1321 atttaacggg aggaaataat tctatgagtc gcttttgtaa atttggaaag ttacacgtta
     1381 ctaaagggaa tgtagataaa ttattaggta tagggcacct ctaataacta ccaattaatt
     1441 cacctcaaaa tgaaattaga aaaaaacagc gaaattttta aatctatttc ttatcgatac
     1501 aaat
//
LOCUS       DAZABD010000216         1417 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.guided.184, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000216 DAZABD010000000
VERSION     DAZABD010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.guided.184"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..211
                     /locus_tag="ACHALG_003054"
     CDS             <1..211
                     /locus_tag="ACHALG_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2588325.1"
                     /translation="GFYSLFKTLQQNPKYHRTLTTYLNTLLSNSFIINQIVEPQPPEN
                     MMDIPGMADEMRRPMMLIVSAKKKM"
     gene            244..1152
                     /locus_tag="ACHALG_003055"
     CDS             244..1152
                     /locus_tag="ACHALG_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HFN2588326.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            1149..1376
                     /locus_tag="ACHALG_003056"
                     /pseudo
     CDS             1149..1376
                     /locus_tag="ACHALG_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          507 a          203 c          309 g          398 t
ORIGIN      
        1 gggattttat tctttattta aaactttgca acagaaccca aaatatcata gaacactgac
       61 cacatatcta aatacactgc tttcaaatag ttttataata aatcagattg tggagccaca
      121 gccgccagag aacatgatgg atattccggg gatggcggat gaaatgcgac gcccaatgat
      181 gctgattgta tcggcaaaaa agaagatgta ataatataga aaaaataaac gaggagtatg
      241 taaatgagat cagaaaaaga aatgatggat ttagtacttt ctttagcaga acaggatgaa
      301 cgtattcgaa ttgtgaccct tgaggggtca cgcgcaaata ttaatatacc taaagatgaa
      361 tttcaggatt atgatattac atattttgta agtgatatag aaccgtttat atctaatgat
      421 gactggctta atcaatttgg gaatataata atgatgcaaa agccggagga tatggaatta
      481 ttcccacctg aagaaaaggg attttcctat cttatgctat ttgatgatta caataaaatt
      541 gatcttacct tattgccctt ggaagagtta gataattacc taaagggcga taaattaata
      601 aaggttctaa ttgataaaga ttgtagaatt aaaagggaca tagttccgac tgatatagat
      661 tatcatgtaa gaaagccaag cgcaagggag tatgatgatt gctgcaatga attttggaat
      721 gtaacacctt atgttattaa aggattgtgc cgtaaggaaa ttttatttgc tattgatcat
      781 tttaatcaga ttgttcgcca tgagctgctg agaatgatat catggaaggt cggcatcgaa
      841 acaggcttta aattaagtgt aggcaagaac tataagttta ttgaaaggta tatatccgag
      901 gatttgtggg agaaactttt gtccacctac cggatggatt cctatgaaaa catatgggaa
      961 gcattatttc tatgccatca attgttcagg gcggtatccg gtgaggtggc ggaaaggctt
     1021 cattatgcct atccggagta tgataggaat ataacaaaat ataccaggga catgtataaa
     1081 aaatacactg gtaaaaccgg ctgcctggat agcacatatg ccgctgatat agaagagagg
     1141 cgggaacagt gattacagaa atgaaagcag ggcacctgaa agatatcgat aaacccagcg
     1201 aaccatttga ggtgataggt aagattatac cgaggtatga aaacgagaat tggaccttta
     1261 cagaattact ctatgaagcg ccatatttaa aaagctacca agacgaagag gatgaagagg
     1321 atgaggaggc agattgcctt gaatatattg acaatactga taagataata tatcttttaa
     1381 cccgtggtgc tagttaattt taatatccta aagaata
//
LOCUS       DAZABD010000217         1349 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2891
            SAMEA115706306-rid22589523.guided.189, whole genome shotgun
            sequence.
ACCESSION   DAZABD010000217 DAZABD010000000
VERSION     DAZABD010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706306
            Sequence Read Archive: ERR13196042
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002289078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002289078.1
            Genome Coverage       :: 115x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 14:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,058
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 47 of 112
            Pseudo Genes (incomplete)         :: 60 of 112
            Pseudo Genes (internal stop)      :: 25 of 112
            Pseudo Genes (multiple problems)  :: 18 of 112
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706306-rid22589523.guided.189"
                     /isolate="VRE2891"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            46..297
                     /locus_tag="ACHALG_003057"
                     /pseudo
     CDS             46..297
                     /locus_tag="ACHALG_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            390..1184
                     /locus_tag="ACHALG_003058"
                     /note="aph(3')-IIIa"
     CDS             390..1184
                     /locus_tag="ACHALG_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HFN2588327.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT          424 a          234 c          322 g          369 t
ORIGIN      
        1 cattttcctc taggaaaaaa ttaactagca ccacgggtta tcttttatat agaagatatc
       61 gccgtatgta aggatttcag ggggcaaggc ataggcagcg cgcttatcaa tatatctata
      121 gaatgggcaa agcataaaaa cttgcatgga ctaatgcttg aaacccagga caataacctt
      181 atagcttgta aattctatca taattgtggt ttcaaaatcg gctccgtcga tactatgtta
      241 tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaag gttttagaat
      301 gcaaggaaca gtgaattgga gttcgtcttg ttataattag cttcttgggg tatctttaaa
      361 tactgtagaa aagaggaagg aaataataaa tggctaaaat gagaatatca ccggaattga
      421 aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg
      481 tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata
      541 aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc
      601 tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga
      661 gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga
      721 ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc
      781 cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc
      841 tggccgatgt ggattgcgaa aactgggaag aagacactcc atttaaagat ccgcgcgagc
      901 tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc
      961 tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga
     1021 gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg atcagggagg
     1081 atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt
     1141 gggagaaaat aaaatattat attttactgg atgaattgtt ttagtaccta gatttagatg
     1201 tctaaaaagc tttaactaca agctttttag acatctaatc ttttctgaag tacatccgca
     1261 actgtccata ctctgatgtt ttatatcttt tctaaaagtt cgctagatag gggtcccgag
     1321 cgcctacgag gaatttgtat cgataagaa
//
